LOCUS       DASWOG010000001         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_55026,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000001 DASWOG010000000
VERSION     DASWOG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55026"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGB93_00005"
     CDS             complement(<1..432)
                     /locus_tag="VGB93_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144544.1"
                     /translation="MVVVLLVAVTAAFFDRINIAVLFTNADFKHAIGVGNNPAMLGLL
                     MTGFVVAYGVSALILSVSSDLFGPRRTLSVIALLLGVIMAIMGGAASYAMMLTGRVLI
                     GLTEGPQFGSTNVAVKQWFPPHERGLASAIWGIGAPLGSMIG"
     gene            680..>1727
                     /locus_tag="VGB93_00010"
     CDS             680..>1727
                     /locus_tag="VGB93_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0144545.1"
                     /translation="MSKGIEQREQSSHVDFTAYLPEHRGLFYGGEWHEPRGGYLETFN
                     PATGESLGQAAEANEADVDAAVEAAHRAFATWRRLAPRERGAILKKIAQVLKTNAEEL
                     ATLDALNCGNPIRELINDVQKGASQIEYFAGLALEVKGEILPTIPDLVNMTVREPYGV
                     CARIVAYNHPLMFAAQRIGAPLVTGNTIILKPPPQAPLSAYRLMELLDGIVPPGVLNM
                     VSGGRTCGAALATHKKTPVVSFVGSVESGKACNSAAAPYLKKVILELGGKNAMIVYPD
                     ADIAKAIDGAVRGMNFSWCGQSCGSTSRLFLHESIHDAVVAGIVEKAKTYKPGNPVDP
                     RTTMGTLISKADLDR"
BASE COUNT          404 a          483 c          520 g          320 t
ORIGIN      
        1 accgatcatc gagccgagcg gcgcgccgat gccccagatc gccgaggcga ggccgcgctc
       61 gtggggagga aaccattgct tgacggccac attcgtggag ccaaattgcg gaccttccgt
      121 gaggccgatc agcacgcgcc cggtaagcat catggcgtaa gatgctgcgc cacccatgat
      181 cgccatgatc acgccgagaa gcagcgcaat aaccgacaag gtccgacgtg gaccgaaaag
      241 atcggaagag acagagagga tgagcgccga aacgccgtag gcgacgacaa agccggtcat
      301 caacaggccg agcatcgccg gattattgcc gacgccgatt gcgtgtttga aatccgcgtt
      361 ggtgaagagg acggcaatat tgatacggtc gaagaatgcg gcggtgaccg cgaccagaag
      421 cacgacgacc ataacccagc gatacgctgc gctttgcacg ccagctgtcg gtaccggcgc
      481 tgccacctga gagggccctg ttactgctga atccgacatc acatattcct cttaggggag
      541 ccaggctccg tcgtagacct tgaacaataa tgtccgattg taatacaata ttcatgcctg
      601 ccaagagcgt ggcatataaa tggctgaatg gaaattgcgt gcgcgccgtg cgggggtagc
      661 aggcaaaagg agacggtgcg tgagcaaagg aatcgaacag cgtgaacaaa gctctcacgt
      721 tgatttcaca gcgtatctac ccgagcatcg cggacttttc tatggcggtg aatggcacga
      781 accgcgtggc ggctatttag aaacgttcaa ccctgcgacc ggcgaaagtc ttgggcaggc
      841 agccgaagcc aacgaagccg atgtcgatgc ggctgtcgag gccgcgcacc gagcctttgc
      901 gacatggcgg cgtctggcgc cgcgtgagcg cggggcgatc ctaaagaaga tcgcgcaggt
      961 tttgaagacc aatgcggaag aactcgccac gcttgatgct ctaaattgcg gcaacccaat
     1021 ccgtgaatta atcaacgacg ttcaaaaagg cgccagccag attgagtatt tcgctggcct
     1081 cgcgctggaa gtgaaaggcg agatccttcc gacaattcca gaccttgtga atatgactgt
     1141 gcgtgagccc tatggcgtct gtgcccgcat cgtcgcgtac aatcatcccc tgatgtttgc
     1201 agctcagagg atcggcgcgc cgctcgtcac gggaaacacc atcatcctga agccgccgcc
     1261 gcaggcacca ttgtctgcct atcgtttgat ggaattgctg gatggaatcg tgccgccggg
     1321 cgtcctgaat atggtgtcgg gtgggcgcac gtgcggcgcg gcgcttgcga cacataagaa
     1381 aacccccgtc gtgtcgtttg tcggcagcgt cgaaagcggc aaggcctgca acagtgccgc
     1441 cgcgccttac ctgaaaaagg tcatcctcga actcggcggc aagaacgcga tgatcgtcta
     1501 tcccgacgcc gatattgcga aagctatcga cggcgcagta cggggaatga atttttcctg
     1561 gtgcggacaa tcctgcggtt cgacgtcgcg cctcttcttg catgaatcga ttcatgacgc
     1621 ggtggttgcg ggcatcgtgg aaaaggcgaa aacctacaag cccggcaatc ctgtcgatcc
     1681 gcgaacgacg atggggacgc ttatttccaa agcggacctc gaccgcg
//
LOCUS       DASWOG010000002         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_311723,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000002 DASWOG010000000
VERSION     DASWOG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311723"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGB93_00015"
     CDS             <1..663
                     /locus_tag="VGB93_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0144546.1"
                     /translation="GFASQEFSTLVDAANQDFFNANQIAAKAANDGQVTSTSARDLAQ
                     AVETIGQIADFIGGVAYQTNLLALNATIEAARCGEAGRGFAVVAGEVKALAQETSRAA
                     ADIATRLGGVRLATEHVVKAVGVTVERIGRIGAITETIREGTALREEAAGKIGEFVSA
                     TAGEAHQLSSTLEAVAQSTGESERIAAEMLQATNSLSEQTERLLIRSRDFCQQIRGRR
                     VA"
     gene            726..1124
                     /locus_tag="VGB93_00020"
     CDS             726..1124
                     /locus_tag="VGB93_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEY0144547.1"
                     /translation="MLRETSDGGRRPARNVLGEPLESCSFNPLTGFFRNGCCDTSRED
                     AGSHTICIVATADFLAFSKAHGNDLSMPAPDYDFPGVQPGDRWCLCAPRWQEAFEAGQ
                     APRVALKATHEGALAYCDLADLKAHAVDLS"
     gene            1367..>1840
                     /locus_tag="VGB93_00025"
     CDS             1367..>1840
                     /locus_tag="VGB93_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY0144548.1"
                     /translation="MPRRTDISTIMIIGAGPIVIGQACEFDYSGTQACKALRDEGYRI
                     VLVNSNPATIMTDPDLADRTYVEPITPEVVAKIIAKERDAVPGGFALLPTMGGQTALN
                     CALSLQKMGVLDKYKVEMIGATAEAIDKAEDRERFREAMSRIGLATPRSHQIKTLI"
BASE COUNT          355 a          577 c          565 g          343 t
ORIGIN      
        1 ggcttcgcgt cgcaggaatt cagcaccctc gtcgatgctg ccaatcagga ttttttcaac
       61 gccaatcaga ttgccgcgaa ggcggcaaac gatggccagg tcacgtccac ctcggcacgc
      121 gatctcgcac aggccgtcga aaccattggc cagatcgccg atttcatcgg cggcgtcgcc
      181 tatcaaacca atctgctcgc actcaacgcc acgatcgagg cggcgcgctg cggcgaggcc
      241 gggcgcggtt ttgccgtcgt cgccggcgag gtcaaagcct tggcgcaaga gacgtcgcgc
      301 gccgcggccg acattgcaac ccggcttggc ggcgtccgct tggcgacgga acatgtcgtc
      361 aaagcggtcg gcgtgacagt tgaacgcatc ggccgcatcg gcgcgatcac cgagacgatt
      421 cgcgaaggta cggcgctgcg tgaagaggcc gccggaaaga tcggcgaatt tgtttcggcc
      481 acggccggcg aagcgcatca actctccagt acgctggagg cggttgccca atcgaccggc
      541 gaaagcgaac ggatcgccgc ggaaatgctg caggcgacca attctttgtc ggaacagacg
      601 gagcggctgc tcatccggtc gcgcgacttc tgccagcaga tccgcgggcg tcgggtggct
      661 taggctttgt tgcgtttgcg caacaaatct gcctttctgc tccacgaacg cgaggcacgg
      721 aatttatgct cagagaaacc tcggacggcg gccgccgacc ggcccgcaat gtccttggcg
      781 agccgctcga aagctgctcg ttcaatccgt tgacggggtt cttccgcaat ggttgctgcg
      841 acacatcgcg ggaggacgcc ggcagccaca cgatctgcat cgtggcaacg gcagattttc
      901 tcgccttctc aaaggcgcac ggcaacgacc tttcgatgcc tgcaccggat tatgactttc
      961 ccggcgtcca gccgggcgac cgatggtgcc tctgcgcgcc gcgctggcag gaagcgttcg
     1021 aggccggtca ggcgccgcgt gtcgcgctga aagcgacgca tgagggcgcc ctcgcttatt
     1081 gcgatctggc ggatctgaag gcccacgcgg tcgatttgtc ctagagcatg tcccgaaaaa
     1141 gttgtgcgac tttttcgatc ggaacatgcc ccaaattatg aattgccgcg atttcttatt
     1201 gaccggatga gtccctccga tccgaaagcg cgctgaaagc cgggggttca cagggcggct
     1261 tttcttcttt agagcaatcg ctctagccca taactcgcaa atttcgccgc cccgcctctg
     1321 cggagcggcc ttccggcgcg cccggatgag gagacgaacc cgacctatgc cccgccggac
     1381 ggatatttcg acaattatga tcattggtgc cggcccgatc gtcatcggcc aggcttgcga
     1441 attcgattat tccggaaccc aggcctgcaa ggccctgcgc gacgagggct atcgcatcgt
     1501 cctcgtcaat tccaatccgg ccacgatcat gactgacccg gatctcgccg accgtaccta
     1561 tgtcgagccg atcacgcccg aggtcgtcgc caagatcatc gccaaggaac gcgatgcggt
     1621 gcccggtggc ttcgccttgc tgccgacgat gggtggccag acggcgctca attgcgcgct
     1681 ctcgctgcag aaaatgggcg tgctcgataa atataaagtc gagatgatcg gcgcgacggc
     1741 ggaagccatc gacaaggcgg aggatcgcga acggttccgc gaggcgatga gccgcatcgg
     1801 ccttgcgacg ccgcgctcgc atcagatcaa gacgctgatc
//
LOCUS       DASWOG010000003         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_293417,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000003 DASWOG010000000
VERSION     DASWOG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293417"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGB93_00030"
     CDS             <1..499
                     /locus_tag="VGB93_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY0144549.1"
                     /translation="SGRGWFQVTAPDGTLLYTRAGDFSTNQNGQIVTQDGYVVSPSMI
                     IPTNALSTTINQTGIVSVMLSGQTQPQQIGQLTLANFANEGGLLPVGNNNFQQTEASG
                     QAVAGIPGDPGFGTINQGFLEASNVDPVKEITNLISAQRAYEMNSKVVQAGDQMEQTL
                     TKNMG"
     gene            611..991
                     /gene="flgA"
                     /locus_tag="VGB93_00035"
     CDS             611..991
                     /gene="flgA"
                     /locus_tag="VGB93_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY0144550.1"
                     /translation="MLPVPSVTIYPGDIIKKDWIVDHDFGAGGPMRGVAIDSRSGLVG
                     KIARHTLLPGLPISTNAVGEPRLIADGQKVRVVFEQDGLIIESYASALQAGGAGDVIS
                     IRNLDSGVTISGIVQTDGTVRVGG"
     gene            991..2088
                     /gene="flgI"
                     /locus_tag="VGB93_00040"
     CDS             991..2088
                     /gene="flgI"
                     /locus_tag="VGB93_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY0144551.1"
                     /translation="MLRICLALVFCLAMFGADAAVRIKDISELRGVRDNQLLGYGLVV
                     GLQNSGDTLLNSTFTQQALESMLDRMGINVRGTAMRTRNVAAVLVTADVPPFVTPGSR
                     IDVTVSSLGDATSLMGGTLVLTSLNGVDGQTYAIAQGQVAVAGYSAEGQAESVTENVP
                     TAGRIANGALIERQVPGELDDISSLTLELNNPDFATAARVADAINAYSQRHYGRQVAR
                     QRDQRSIDFDAPPEVNRARFIAELGELDVEPDMPARVVMDARSGAVVISQDVQISTVA
                     VTYGNITVRVTETPQVSQPAPFSRGRTVVVPNTQINVDQPDATIAIVHGPSLRTLVAG
                     LNQIGLKPSGIIAILQAIKAAGALQADLVVE"
     gene            2223..2798
                     /locus_tag="VGB93_00045"
     CDS             2223..2798
                     /locus_tag="VGB93_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HEY0144552.1"
                     /translation="MQAQTRASGDDSAAPSSADADFPSQPAQDPIDLHVETQKKHAAA
                     VARAKAEARAHALRSLPEVRMFCANNVSTADAARIAWQASKLEEVEARLQEKIAELEA
                     KRAEFQEWLRKRDEAMKMARQSVVEIYSKMDPEAAASELVQMDDAMAAAVLSKLNPRT
                     ASAILDQMDPARAAHLTDEMVGADSGDGKRS"
     gene            2798..3499
                     /gene="flgH"
                     /locus_tag="VGB93_00050"
     CDS             2798..3499
                     /gene="flgH"
                     /locus_tag="VGB93_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY0144553.1"
                     /translation="MRMLLLSAGALLLAGCAADLKEVGREPSMSPVGTGLSADVMPMP
                     TSVFAQNNRSTSGAIWDGSRADIFTDPRASRIGDVITINIAINDSATFGNASGRNMNA
                     EVKGGLNYQVQNSGGQTQWYPQYDFTSSSNAQGQGSIDRSEKIQLSVGAVVTDVLPNG
                     NLIVSGSQEVRVNYELRVLNIAGIGRPRDISKDNTIAYNKIAEARISYGGRGRIMEVQ
                     QPAWGQQIFDKFKPL"
     gene            3550..>3903
                     /locus_tag="VGB93_00055"
     CDS             3550..>3903
                     /locus_tag="VGB93_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144554.1"
                     /translation="MAEDDITDVEAAAVPAPQLFPSLLPWLIAMGGISVLAIIIGAVV
                     GLAMASVARRDAQAAVLAAQPAYSNVSLVDLPPVITNLADPTSAWVRLEAAVVVDKQT
                     TTKPDVLGAEIADDIL"
BASE COUNT          795 a         1124 c         1190 g          794 t
ORIGIN      
        1 cagcggccgc ggatggtttc aggtcacggc gccggatggc acgttgctct atacgcgcgc
       61 tggcgatttt tcgaccaatc agaatggcca gatcgtgacg caggatggct atgtcgtcag
      121 cccgtcaatg atcattccga ctaacgccct tagtacgaca atcaatcaga ccggcatcgt
      181 ctccgtcatg ctctctggcc agacacagcc tcagcagatc ggtcagctga ccttggcaaa
      241 ctttgccaat gagggcggtc ttttgccggt cggcaacaat aactttcagc agacggaagc
      301 gtccggccag gcggttgcgg gtattcccgg cgatccgggg tttggcacga taaaccaggg
      361 cttcctcgag gcttcgaatg tggatccagt caaggagatc acaaatctca tctcggccca
      421 acgcgcctat gagatgaatt cgaaggtcgt gcaggccggg gaccagatgg agcagacgct
      481 caccaagaat atggggtgat gcatgacctc tcaatcgaga cgcctgaacg ggcgggttag
      541 aaacaggcgt cgtctcgctg cgcccgtttt attcctggtg ctttttgtat cgcccgccac
      601 tgcgggaaat gtgttgcccg tgccatcggt gacgatctat ccgggcgata ttatcaagaa
      661 agattggatc gtcgatcatg atttcggtgc cggtggcccg atgcgtgggg tagcaatcga
      721 ttcgcgcagc gggctcgtcg gcaaaatcgc gcgccataca ttgctgccgg ggctaccaat
      781 atcgaccaat gccgttggtg agccgcgact catcgcggac ggtcagaaag tcagggtcgt
      841 ttttgagcaa gacgggctta tcatcgaatc ttatgcgtca gctctccagg caggcggcgc
      901 gggcgatgtt atctcgatac gcaatctcga tagcggcgtg acaatttcgg gaatcgtgca
      961 gaccgatgga accgtgcggg tgggcggata atgctgcgta tctgcctcgc cctggttttt
     1021 tgccttgcaa tgttcggcgc cgatgcggcg gtccgaatca aggatatttc cgaactgcgc
     1081 ggggtccgcg acaatcagct tcttggttac ggcctcgtcg ttggcctgca gaacagcggc
     1141 gacacattgc tgaattcgac atttacacag caggcgctag aatccatgct tgatcgcatg
     1201 ggcatcaatg tgcgcggcac cgcgatgcgt acacgcaatg tcgctgcggt tctggtcacg
     1261 gccgatgttc cgccttttgt cacaccgggt tcgcgcatcg atgtgaccgt gtcatcgctc
     1321 ggcgacgcga catccttgat gggcggtaca cttgtcctga cctcgctgaa cggcgtcgat
     1381 ggtcagactt acgcgatcgc gcaagggcag gtcgccgttg cgggctattc ggcggagggc
     1441 caggcagaga gcgtcaccga gaacgtgccc acagcggggc gaatcgccaa tggcgcgctg
     1501 atagagcgcc aggtgccggg cgagctggac gacatcagct ctttgacact cgagctgaat
     1561 aatcctgatt tcgcgaccgc cgcacgagtc gccgatgcca tcaacgccta ttcgcagcgc
     1621 cattacggcc ggcaggttgc ccgtcagcgc gatcaacgct cgatcgattt cgatgcgccc
     1681 ccggaagtca acagagcacg tttcattgct gaacttggcg agctcgatgt cgagccggat
     1741 atgccggcac gcgtcgtgat ggatgcgcgc agcggcgcgg tcgtcatcag tcaggatgtg
     1801 cagatttcga cggtcgccgt cacctacggt aatattacgg tgcgcgtgac ggagacgccg
     1861 caagtctcac agccggcgcc gttctcgcga ggcaggaccg tcgtcgtccc gaatacgcag
     1921 atcaacgtcg atcagcctga cgcgacaatt gcgatcgtcc acggcccgag tctgcgaact
     1981 ctggtcgctg gcctgaatca gatcggcctc aagccgagcg gcattattgc catcctccag
     2041 gccatcaagg ctgcgggtgc gttgcaggcc gatctcgtcg tcgaataatc actcaaaaca
     2101 gccagcccgg cacaagcttc ttcttgcagt ctcgcgagcg cgacggcgct ttgcggctgg
     2161 cgggcgggaa tatgcgacac gcttcatttg catttttgat cttttgcaca tgcgtaacga
     2221 gcgtgcaggc gcagactcgc gcctccggcg acgattcggc cgcgccgtcc tctgcggacg
     2281 ctgattttcc atcgcaacca gcgcaagacc cgattgatct gcacgtcgag acgcagaaga
     2341 agcatgctgc cgccgttgcg cgcgctaagg ccgaagcgcg agcgcatgcg cttcgcagtt
     2401 tgcccgaagt gcggatgttc tgcgccaaca atgtttccac cgcggatgcg gcgcggatcg
     2461 cctggcaagc gtcgaagctt gaagaagtcg aggcgcgttt gcaggaaaaa atcgccgaac
     2521 tcgaggcaaa gcgtgcggaa tttcaggaat ggctacgcaa gcgcgacgaa gccatgaaga
     2581 tggcgcgcca aagcgtcgtc gagatctatt cgaaaatgga tccggaagct gccgcgagcg
     2641 agcttgtgca gatggatgat gctatggccg cggccgtcct cagcaaattg aatccccgta
     2701 ccgcgagcgc gatcctcgac cagatggacc cggctcgcgc ggcgcatctg acagacgaaa
     2761 tggtaggcgc cgactctggc gacggaaaga gatcctgatg cgtatgctgc ttctctcggc
     2821 tggtgcgctg ctccttgcag gatgtgcggc cgatctcaaa gaggttggcc gcgagccttc
     2881 gatgagtccg gtcggcaccg ggctttcggc ggatgtgatg cctatgccaa cctccgtctt
     2941 cgcgcaaaac aatcgcagca caagtggcgc gatctgggat ggtagccgtg ccgatatttt
     3001 taccgatcct cgggcctcga gaattggcga cgtgatcacc atcaatattg cgatcaatga
     3061 cagtgcgacc tttggcaatg cctccggccg caacatgaac gctgaagtca aaggtggcct
     3121 gaactatcag gtccagaaca gcggcggcca gactcaatgg tatccgcaat acgacttcac
     3181 gtcgtcatcg aacgcgcagg ggcagggctc gatcgatcgt tcggagaaaa tccaattgtc
     3241 ggtcggcgcg gtggtcaccg acgttctgcc gaacggaaat ctgatcgtca gcggctcgca
     3301 agaggtgcgc gtcaattacg agctgcgcgt gctcaatatc gccggcatag ggcggccgcg
     3361 cgatatctcg aaagacaata cgatcgccta caacaagatt gctgaagcgc gcatttccta
     3421 cggcggccgc ggccgcatca tggaagttca gcagccggca tggggccagc agatttttga
     3481 caagttcaaa ccgctttagc gatgaaactt tgcgccacca acgggccgcg gtgctttcgg
     3541 acgtgataaa tggccgagga tgatatcacc gatgtagagg ccgcggcagt tccggcgccg
     3601 cagctttttc cgtctctgct gccctggctt atcgccatgg gcgggataag cgtccttgcg
     3661 atcatcatcg gagccgtcgt aggcctcgca atggcctccg tggcacggcg tgacgcacaa
     3721 gccgcggtcc tcgcggcaca gccggcttat tccaatgtga gtctggtcga tctgccaccg
     3781 gtcatcacca atcttgccga cccgacgagt gcgtgggtga ggcttgaagc ggccgttgtc
     3841 gtggataagc agacaacgac gaagccagac gtgctcggtg cggaaattgc cgatgacatt
     3901 ctc
//
LOCUS       DASWOG010000004         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_293498,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000004 DASWOG010000000
VERSION     DASWOG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293498"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VGB93_00060"
     CDS             complement(<1..158)
                     /locus_tag="VGB93_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0144555.1"
                     /translation="MADLSSLSEAQMRRIEPYFPLSHGIARVDDRRVISGIVFVIRNG
                     LRWRDAPPG"
     gene            279..1508
                     /locus_tag="VGB93_00065"
     CDS             279..1508
                     /locus_tag="VGB93_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0144556.1"
                     /translation="MIRRSWMTGTSIETTLELWSSSLREVKARIKPLFTQERVAASAG
                     LFLDGLLGDERRKTGWMRAEAAGDKGPWRQQAILGRGRWDADDLRDIVRDYVVEHLAD
                     GDAVLVIDETGFLKQGKASCGVARQYTGSAGKITNCQIGVFAAYVSRHGHAFIDRALY
                     LPKVWTDDQARMAAVHVPDKMSFATKPALALRMIERAIAANMPFGWVAADCVYGVGDI
                     EIALRRKAKGYVLGVSAKHRFASWSRKLAVAGTAEEIAGNLDPSAWLRLSAGDGTKGE
                     RLYDWAYCELADLEAAEYNDERTGLWTRGLLIRRTIADGDMAFFTTWCPAGTSIETLV
                     RIEGHRWAIEDSFETAKNELGLDHNETRSWHGWYRHVSLVMLAFAMMAVIRHHANKTS
                     PQKTKITGKGLLKRVVH"
BASE COUNT          380 a          502 c          517 g          378 t
ORIGIN      
        1 ccgggcggcg catcgcgcca gcgcagaccg tttctgatga cgaagacgat gccactgatc
       61 acccgccgat cgtcaaccct cgcaatcccg tgcgacaatg gaaaatacgg ttcgatccgg
      121 cgcatctgcg cctccgacag cgacgacaga tccgccatgg tagtgcctcc cgacaccacc
      181 attgaattaa tccgctaatc ctcatgcaac aatttaatgg gtcctgagcc tagcattaca
      241 gttgcaattt ttttggatct ctgaatccat aggtcaccat gattcggaga tcatggatga
      301 ctggtacatc gatcgagaca acgctggagc tttggtcatc gtcgttgcgt gaagtgaaag
      361 cgcggatcaa gcctttattc acgcaggagc gtgtggcggc ttctgcgggg ctttttcttg
      421 acggcctgct tggcgacgaa cggcgcaaga ctggatggat gcgcgccgag gccgctggcg
      481 ataaaggtcc gtggcgtcaa caggccattc tcggacgcgg ccgatgggac gcggatgacc
      541 ttcgtgacat cgtgcgtgat tatgtcgtcg agcatctcgc tgatggcgat gcggtgctcg
      601 tcatcgatga aactggcttc ctgaagcaag gtaaggcatc ttgtggcgtt gcgcgtcaat
      661 acacgggttc cgcgggtaag atcaccaact gccagatcgg cgttttcgcc gcctatgttt
      721 cccggcatgg ccatgctttc atcgaccgcg ctttgtatct ccccaaagtg tggacggatg
      781 atcaagctcg catggccgcg gtccatgtgc cggataaaat gagtttcgcg acgaagccgg
      841 ccctcgcgtt gcgcatgatc gaacgggcca tagccgcgaa catgccattt ggttgggtgg
      901 cagccgattg cgtctatggt gtcggcgata tcgagatagc tctacggcgc aaggccaagg
      961 gctatgttct cggtgtcagc gccaagcatc gttttgcatc ttggagccgc aagctcgcag
     1021 ttgcaggcac ggctgaggag atcgcaggca atcttgatcc ttcggcttgg ctgcgtttgt
     1081 cagcgggcga cggtaccaag ggcgagcggc tttatgactg ggcgtattgc gaactcgccg
     1141 atcttgaggc tgccgaatac aatgatgaaa gaaccggtct gtggacgcgc ggtctcctga
     1201 tccgtcgcac gatcgcagac ggtgatatgg ccttcttcac cacatggtgc ccggctggaa
     1261 caagcatcga aacattggtc aggatcgaag gacatcgctg ggcgatcgaa gacagtttcg
     1321 aaactgccaa aaatgaactc ggcctcgacc acaacgagac gcgctcctgg cacggttggt
     1381 atcggcacgt ctctttggtg atgctggctt tcgccatgat ggccgtcatc cgccatcatg
     1441 caaacaaaac gtcgccccaa aaaacgaaga tcacgggcaa aggattgctc aagagagtcg
     1501 ttcactgatc cgttggtcag ttcaggaaat ccgccgcatc gccatcaggc ttgcgcaaag
     1561 acggattaag cccgcgcgcg tcatcgcatg gtcgctctgg cgaagagccc atcaggccga
     1621 agcccgacgt gcgcatatca aatctaaatc gcaactgtaa tgctaggtcg gaccgctgcg
     1681 gacgcccata aacgtgcgta gaattgcggc acgtctgccc ggcgtctgcg actagcgagc
     1741 cctccgcaac ttcacgacgc attcgccgcg ccgccga
//
LOCUS       DASWOG010000005         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_91886,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000005 DASWOG010000000
VERSION     DASWOG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91886"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VGB93_00070"
     CDS             <1..117
                     /locus_tag="VGB93_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0144557.1"
                     /translation="MNEASTKISALQVQQQLGVQALSIANSNTQLILKLFGQ"
     gene            239..1885
                     /gene="fliF"
                     /locus_tag="VGB93_00075"
     CDS             239..1885
                     /gene="fliF"
                     /locus_tag="VGB93_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY0144558.1"
                     /translation="MAALEQARRFWENLMKLGTRRLIGLGVTGLMIFLVTGLAGYYLS
                     RPTQELLYSGLDREDVSRIASALKEANIDFDVSSDSTSVYVRYGQTAPARMLLAEKGL
                     PNSPNAGNELFDKLGSLGLTSFMQQVTRVRALEGELARTIQLMSAVKAARVHLVLPDD
                     GSFRRESEPPSASVVVRTDVPDDVAVAKAIRHLVAAAVPGMKPEQVTVLDTDGMLLAS
                     GDDTGDSAPGQMLSLEKTFSKEIQDNIGRTLTPFFGFTNFQISVATRINTDKRQTDET
                     VFDPDSRVERSVRVVRSNETSQNSSNQPATTVTQNLPPEKTDSDNGKQSNEENQKRDQ
                     TTNYEVSSKTIQTISGGFAVEHISVAVLVNRASLLASLGGKPTQEAIDKQLSDIGQIV
                     ASAAGANTSRGDTIKVSAVDFVESGRNLAPVPPPTWQEILLRQTGTAINGGVILLVAF
                     LGLWFGVRPVVKALSPPAGIGGSAGYLGADSLELPEPSLQMPEGMPMFSGMAPEPNLI
                     EDLTDNNKRTPVKRLEQIVEFDEQQAVNILKLWMRQRSPA"
     gene            1882..2508
                     /locus_tag="VGB93_00080"
     CDS             1882..2508
                     /locus_tag="VGB93_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144559.1"
                     /translation="MIARPVAEFLVQMELGASEPAALFIPTDVDLAPVWSEETKDDPQ
                     AQIDTARDEGYAAGVEAAAAEHAGQLARAHEQFAAELATARETWVRGEGDSLREQLTT
                     ALLAMEETFADAVGRVLQPFVVAALRQRMIDKLIDGIRTIAGSADKIVIQISGPADLL
                     EILRQHLQASPAAIEYVTLDSVDVRVVAQQTSIDTQLKAWIDLIATEG"
     gene            2512..>3168
                     /locus_tag="VGB93_00085"
     CDS             2512..>3168
                     /locus_tag="VGB93_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY0144560.1"
                     /translation="MADNEKHEILIIKRSRASAHEPHGGAWKIAFADFMTAMMALFLV
                     LWLVNATNTKTKASLARYFNPVDLVDMSTHKRGLHDPHSGPETDGAADGASFPKSAAK
                     AAKKTGSSSGFEDGAAGPSDSKINQDAVARTVEPPPTHSEEALFRDPYAVLAEIAASG
                     SSGQAASGEQLDQQDNDSSGLTTDTYADPFKPVDKTAETTPVASTPAPQAAAIQAANN
                     A"
BASE COUNT          696 a          901 c          939 g          632 t
ORIGIN      
        1 atgaacgaag cttcgacgaa aatttcggcc ctgcaggttc agcagcagct cggtgttcag
       61 gcgttgtcga ttgccaattc caacacccag cttatcctga agctcttcgg ccaataataa
      121 ttgacgtagg tttccttcgc gaggcggccg cgcttacgag cgcggccgtt ttcgtttgag
      181 caatccccgc gcaagcttct gaccgcacat tgcatgaaag cggcagcgga ggcggggaat
      241 ggcagcgctc gaacaagcga gacgcttctg ggaaaatctt atgaagctgg ggacgcggcg
      301 gcttatcggc cttggcgtaa ctggcctcat gatctttctt gtgacgggcc ttgccggcta
      361 ttatctcagc cggccgacgc aggaactgct ttatagcggc ctggatcggg aagacgtcag
      421 ccgcatcgca tctgcgctca aggaagccaa catcgatttc gatgtgagct ccgacagcac
      481 gtctgtatat gttcgctatg gacaaactgc gccggcgcgc atgttgttgg cagagaaggg
      541 gctgccgaac agccccaacg cgggcaacga gctttttgat aagcttggtt cgctcggtct
      601 cacatctttc atgcagcagg tgacgcgcgt tcgcgcactt gagggcgagt tggcccgaac
      661 gatccagttg atgagtgctg tgaaagcagc gcgcgttcat ctggtgttac cagacgatgg
      721 ttcgttccga cgcgagagcg aaccgccatc ggcctcagtt gtggtgcgca cggatgtgcc
      781 ggatgatgtt gcggtcgcca aagcgattcg ccatcttgtc gctgcggcgg ttcccggcat
      841 gaagccggag caggtgacgg tgcttgacac cgatggaatg ctgctcgctt cgggcgatga
      901 cacgggcgat agcgcgcccg gccagatgtt gagcctggaa aagaccttct ccaaagaaat
      961 acaggataac atcggccgga ctttgacgcc gtttttcggc ttcaccaatt tccagatcag
     1021 cgtcgcgaca cgcatcaata ctgacaagcg gcagacggac gaaacggttt tcgatcccga
     1081 ttctcgtgtc gaaaggtcgg ttcgcgtcgt caggtcgaat gagacttcgc agaattcaag
     1141 caatcagcca gcgacgacgg tcacgcagaa tctgccgccg gagaagacgg attccgataa
     1201 cggtaagcaa tcgaacgaag agaatcagaa acgcgaccag acgaccaatt acgaggtctc
     1261 gtcgaagacc attcagacga tcagtggtgg cttcgcggtg gagcacattt cggtggcggt
     1321 cctggtcaat cgcgccagtc tccttgcttc gcttggtggc aagcccacac aagaggcaat
     1381 cgataagcag ctctctgata tcggacagat tgttgcatcg gctgcgggtg caaacacgtc
     1441 gcgcggcgac acaatcaaag tttcggccgt ggatttcgtc gaatccggcc gcaatcttgc
     1501 ccccgtcccg ccgcctactt ggcaggaaat tctgctgcgc cagacgggga ccgcgatcaa
     1561 tggcggcgtc atattgcttg tggcgttctt gggtctctgg ttcggcgtga ggccggtcgt
     1621 aaaggctttg tcgccgccgg caggcattgg cggatccgct ggttatctcg gcgccgacag
     1681 cctcgaactc cccgagccgt cgctgcaaat gcccgaaggg atgccgatgt tctccggcat
     1741 ggcgccggag cccaatttga tcgaagatct taccgacaat aacaaacgca cgccggtgaa
     1801 acggcttgag cagatcgttg aatttgatga gcaacaagcc gtcaatattc ttaagctctg
     1861 gatgcggcaa cggagtccag catgatcgcc cggccggtcg cggaatttct cgtccaaatg
     1921 gagttgggcg ccagcgagcc ggccgctctg ttcataccga cggacgtcga tttggctccg
     1981 gtatggtcgg aggaaacgaa agacgatcca caagcgcaga tcgataccgc gcgcgatgag
     2041 ggctatgccg ctggcgtcga ggcggcagcg gcagaacatg ctgggcaact cgcacgcgca
     2101 catgaacaat tcgccgccga gcttgcgact gcgcgcgaaa cttgggtgcg tggagagggc
     2161 gacagcctgc gcgagcagct cacgacagcc ttgctcgcga tggaggaaac attcgccgat
     2221 gccgttggcc gcgtcttgca acctttcgtc gttgccgcgc tgcgccagcg gatgatcgat
     2281 aaattaattg acggcattcg caccattgct ggaagcgccg acaagatcgt gattcaaatt
     2341 tccgggccgg ccgatcttct tgaaatttta cgtcaacatc tccaggcctc accagctgcg
     2401 atcgaatatg tgacgttgga tagcgtcgat gtccgtgtcg tcgcccaaca aacatcgatc
     2461 gatacacagc ttaaggcctg gatcgatttg atcgccacgg aaggatagcg cgtggccgac
     2521 aacgaaaagc atgaaattct gatcatcaag cgttcgaggg caagcgcaca cgagccacat
     2581 ggcggcgcct ggaagatcgc ttttgccgac ttcatgacgg cgatgatggc attgttcctc
     2641 gtgctttggc tcgtcaacgc caccaacaca aaaacaaaag catcgctcgc gcgttacttc
     2701 aacccggtcg atctcgtcga tatgtcgact cacaagcgcg ggttacacga tccccacagc
     2761 ggccctgaaa ctgatggagc cgccgatggg gcatcgttcc caaagtcagc ggccaaagcg
     2821 gcgaaaaaga caggcagctc atcgggtttt gaggatggcg ctgccggccc aagcgattcg
     2881 aagatcaatc aggacgccgt tgcgcgtact gtcgagccac cgccgacgca ttccgaagaa
     2941 gcgcttttta gagatcctta tgccgttctc gcggagatcg ccgcgtccgg ttcgagcggc
     3001 caggccgcat ctggggagca gctcgatcag caggacaacg actcgagtgg attgacgacg
     3061 gacacgtatg ccgatccatt caagccggtg gacaagaccg ccgagacgac accggtcgcc
     3121 tccacgcccg caccgcaagc cgcagccata caggctgcga acaatgcg
//
LOCUS       DASWOG010000006         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_220224,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000006 DASWOG010000000
VERSION     DASWOG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220224"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VGB93_00090"
     CDS             complement(<1..1252)
                     /locus_tag="VGB93_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144561.1"
                     /translation="MSAESAAPHAVLPADSVLPGVDPHGDVRPADVATGVIIGRTSEY
                     FDFFVYAIASVLVFPKWVFPYASPLNATLYSFVVFAIGFLARPIGDAIFLSVERAWGR
                     GVKLTAALFLMGASTVSLGFLPGYAQVGWVSALILAILRICQCMAFGGVWDGLPSLLA
                     IYSPEKQRGWYAMIPQLGAPLGLIVASGLFAYFIAYLSPQDFYDWGWRYPFFVAFAIN
                     VVALFARLRIVAAPEYREKFEALELQAAPVIETIEAEGRNILIGVFAPLASFAMFHMV
                     TVFPLSWVMLFTKQTPVTFLIIELIAAVFGVVAIVASGYLANRVGRTRVLIVTAAAIA
                     AFSGFAPQLLNTGVVGETSYMIIGFILLGLSFGQASGTVASNFAPIYRYSASALTSDL
                     AWLFGAGFAPFLALALSARFGVMAS"
     gene            1454..>2131
                     /gene="cyoA"
                     /locus_tag="VGB93_00095"
     CDS             1454..>2131
                     /gene="cyoA"
                     /locus_tag="VGB93_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEY0144562.1"
                     /translation="MRRLRSIVLLSCLALLGGCKAVVLDPSGDVAIQQRDALLDSVWL
                     MLLVIVPVMALTVLFAWHYRKSNTSARYEPDWDHSTQLELVIWGAPLLIIIALGAITW
                     TGTHLLDPYRPIDRIAEGRPLPANAKPLEIAVVALDWKWLFIYPQYRIATLNQVSAPI
                     DRQVEFRITSSGVMNAFYMPAMAGMIYAMPGMETQLHAVMTQTGTYAGLSANYSGDGF
                     AGMHFPYT"
BASE COUNT          487 a          654 c          635 g          355 t
ORIGIN      
        1 ccgaagccat gacgccgaaa cgcgccgaaa gggcgagcgc gagaaacggc gcgaagccgg
       61 cgccgaacag ccaggcgaga tccgacgtca gggccgaggc ggaatagcga tagatcggcg
      121 cgaagttcga cgcgaccgtg cccgaggcct ggccgaacga caaaccaagc aaaatgaagc
      181 cgatgatcat gtagctcgtt tcgccgacaa caccggtatt cagcaattgc ggcgcgaagc
      241 cactgaacgc ggcgatggcg gcggcggtaa caatcaaaac gcgcgtgcgg ccgacgcgat
      301 ttgcgagata gccggatgcc acgatggcga ccacaccgaa taccgccgca atgagctcga
      361 tgatcagaaa tgtcaccggc gtctgcttcg taaagagcat gacccaggac agcggaaaca
      421 ccgtcaccat atgaaacatc gcgaaactcg ccagcggcgc aaatacgccg atgaggatgt
      481 tgcggccctc cgcctcgatc gtctcgatga caggtgcggc ttgcagttcg agggcctcga
      541 acttctcgcg atactcaggc gcggccacaa tccgcagccg cgcaaacagc gccacgacat
      601 tgatcgcgaa agcgacgaag aacggatagc gccagcccca atcgtaaaag tcctgcggcg
      661 acagatacgc gatgaaataa gcgaagaggc cactcgccac gatcagcccg agaggtgcgc
      721 cgagctgcgg aatcatcgcg taccagccgc gttgcttttc tggcgagtag atggcgagca
      781 gcgacggcag accatcccaa acgccgccga acgccatgca ctggcagatg cgcaagatcg
      841 ccagaatgag tgccgaaacc cagccgacct gcgcataacc cggcaaaaag ccgagcgaaa
      901 cggtggaggc gcccatgaga aaaagtgcgg ctgtcagttt cacgccgcgg ccccaggccc
      961 gttcgactga caggaagatc gcgtcgccga tcggccgcgc caggaagcca atggcgaaaa
     1021 ccacgaacga ataaagcgtg gcgttcagcg gcgaggcata gggaaagacc catttcggaa
     1081 agacgaggac cgacgcgatc gcatagacga agaagtcgaa atattccgac gtgcgcccga
     1141 tgatcacacc cgtcgcgaca tcggccggcc tcacatcgcc gtgaggatca accccaggca
     1201 atacggaatc ggccgggaga actgcgtggg gcgctgcgga ttcggcgctc atgggtcgat
     1261 cttactccgt agtggcaagg ccattgcaga cggtcacgca agggcccaca aggatttcgc
     1321 ttcttttctg ctgcaacgcg ctatcggggc attgggcaaa ttgtccaata gccgctcttt
     1381 tgcagtgcag ctagccagtc atgacaataa ccgtccggcc aaagggcgga ccggaccgaa
     1441 ggatgggatt gccgtgagac gtctgcgctc catcgttttg ctttcgtgcc tcgcgctcct
     1501 cggcggctgc aaagcggttg tactggatcc ctccggcgat gtcgctattc agcaacgcga
     1561 tgccctgctc gattccgtct ggctgatgct gctcgtcatc gtcccggtga tggcgctgac
     1621 agtgcttttt gcctggcatt accgcaagtc caatacatcc gcccgctacg agccggactg
     1681 ggatcattcg acgcagctcg aactggtcat ctggggggct cccctgctca ttatcatcgc
     1741 gctgggtgcg ataacctgga cgggtacaca cttgctcgac ccctaccggc cgatcgatcg
     1801 catcgccgag ggcaggccgc tacctgcgaa tgcgaagccg cttgaaatcg ctgtggtggc
     1861 gctggattgg aaatggctct tcatctaccc gcaatatcgc atcgcgacac ttaatcaggt
     1921 tagtgccccc atcgaccggc aggtcgagtt ccgcatcacc tcttccggcg tcatgaacgc
     1981 gttctacatg ccggcgatgg cgggcatgat ctatgccatg ccggggatgg aaacgcagct
     2041 tcacgccgtg atgacccaga ccggcaccta tgcgggcctt tcggcgaatt acagcggtga
     2101 cggcttcgcc ggaatgcatt tcccctacac c
//
LOCUS       DASWOG010000007         9583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_201890,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000007 DASWOG010000000
VERSION     DASWOG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201890"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..578
                     /locus_tag="VGB93_00100"
     CDS             192..578
                     /locus_tag="VGB93_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY0144563.1"
                     /translation="MDFQIAITGFVALYIFMLAAFAGWVIIGHVPAILHTPLMSGSNF
                     VHGIVVVGGLFALLNATNVTQQAIGFFAVLLGAGNAAGGYAVTHRMLAMFQTSGQQTK
                     PAKSSHHAKSSHTKSGHPKTHHAKKA"
     gene            588..2039
                     /locus_tag="VGB93_00105"
     CDS             588..2039
                     /locus_tag="VGB93_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0144564.1"
                     /translation="MLTVIPQLTIGVVDLVAAFLFMFGLKRMSSPVTAPSGIFVAGLG
                     MLVAILASFLYAFDVSAAARPHLLVNIGLAAVALVVGGGIAWWSGRKVAMTAMPQMVA
                     LYNGMGGGAAGAIAAVELFGDKTHGATQLVVTLLGALIGAVSLSGSLIAWAKLDGVIG
                     KPLRVGGQQAINGVVMLATLAVGGYLIFVVLGGAVPLIPLPALIGLFFGLALVFGVLM
                     TLPIGGADMPVVISVYNAFTGLAVGLEGFVLQNPALMIAGMVVGAAGMLLTLLMATAM
                     NRSVANVLFTNFGDVPKHAQGDIKGSLKPVEASDAGIFMRYASKVIIVPGYGLAMAQG
                     QQKLYEFVKLLQAGGVTVKFAIHPVAGRMPGQMDVLLAEAGVPYDLIFQLEDINDDFA
                     SADVALVIGANDVVNPAARTDKSSPIFGMPILNADKAKKVYVVKRGEGKGYAGIQNAL
                     FYGDNCDMVYGDAQAVLVKMIEAVRGLGLLAAA"
     gene            2090..3187
                     /locus_tag="VGB93_00110"
     CDS             2090..3187
                     /locus_tag="VGB93_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY0144565.1"
                     /translation="MTRKMQAAIVEQFGKPLVFQEWDIPTPGPGQILVKTEACGVCHT
                     DLHAASGDWPLKPKLPFIPGHEGIGLVVALGSGVTIVKEGDRVGVPWLYSACGHCEYC
                     LSAWETVCGEAQFGGYTKNGGFAEYILADPNYVAHVPAGLGAQEAAPLICAGITTYKG
                     IKETKARAGEWIAISGAGGLGHLAIQYAKVMGFQVCAVDIDDGKLAHAKKLGADVVVN
                     AKHGDPAAAVKRATDGGAHGVLITAPSLPAFKQGVGMTRKRGTCVLVGLPPGDFPLPL
                     FDVVANCITVRGSFVGNREDMAEALHFGAQGKVKADIELQPLSAINDVFNRLEHGDVP
                     SRVVLDFVGAEALGHQSKAAAGKTRETANIG"
     gene            3472..5319
                     /locus_tag="VGB93_00115"
     CDS             3472..5319
                     /locus_tag="VGB93_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0144566.1"
                     /translation="MSPTCTDPDRPMRIEDYGLVGDCKTAALVGWNGSVDWLCWPRFD
                     GAACFAALLGSAKHGRWSIVPADGSCSTSRSYRGDTMVLETLFETADGAFAIIDFMPV
                     DQPHSSLIRIVEGRRGRVPVRMNLTLRFDYGSSIPWVTRLPESDGIVAIAGPSLAVLR
                     TPVALRGEDLSTSADFSVDVGERVPFVLTYGESHRPPPLPVDVDAALQQTEKFWRDWA
                     ARCSYKGQWRAAVLRSLLTLKAQTFAETGGIIAAPTTSLPEQLGGQRNWDYRFCWLRD
                     ATLTLVALMGAGYYDEAKAWRDWLRRAVAGTPSDVQIMYGVAGERRLFEREIPWLPGY
                     QGAAPVRIGNAASEQLQLDVFGEIMDALHVAREIGFAHLSTDWSLQCCALEHLTAIWR
                     QPDDGIWEMRGGRRHFTHSKIMAWVAFDRSIRDAEKYGFDAPLVHWRAERDEIRRVVC
                     EQGYDRKRGTFTQSFGSPELDASLLLISDVGFLPIDDPRVAGTIAAVERELCVDGFVL
                     RYRTESGADGLPSGEGVFLPCSFWLIDVYQKQGRNAEAHAMLDRLLALRNDLGLLSEE
                     YDVIAARQVGNFPQAFSHLSLVRTVLSLDRRKPIKNRPGIPTLDESVKR"
     gene            6354..6476
                     /locus_tag="VGB93_00120"
                     /pseudo
     CDS             6354..6476
                     /locus_tag="VGB93_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
     gene            complement(6609..7214)
                     /locus_tag="VGB93_00125"
     CDS             complement(6609..7214)
                     /locus_tag="VGB93_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144567.1"
                     /translation="MMALYNYHPSINAQLQTTCESATGAPLHCKTTRIAADAVHLVYD
                     TKAGQSIEASRKQAESLSEGTGVRLHVEELGSLAGTIAAKKREGFDVRIDSEYRPAVR
                     DRLARMAANYAVAVERGAGATSKILTVEPSIKRCQFLDHTGTLREGTLVNISEDDALI
                     RARIIPPERSRIVLRGAIHRAADVIRVFEIGFALKFLTSRP"
     gene            complement(7642..8784)
                     /locus_tag="VGB93_00130"
     CDS             complement(7642..8784)
                     /locus_tag="VGB93_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0144568.1"
                     /translation="MLDMDLAISAYLRAAEAARKRAEEAVLAQEREDVSASVGKAMSS
                     LSDGDLTCRLADDIPGEYQQLRIDFNSSMEKLQQTVLAVSANAQAIRSGTEQIAVAAD
                     DLSHRTERQAASLEETAAALDEITTTVKKTAENANYAHVTVSAAKMEAEKAGVIVGEA
                     MHAMSGIEKSSHQISQIISVIDEIAFQTNLLALNAGVEAARAGDAGRGFAVVAMEVRG
                     LAQRSAEAAKEIKGLISASTAQVDQGVHLVEETGKALQRIVEQVIEINQVVSNIATSA
                     QEQAAGLHQVNIAVNEMDKVTQQNAGMVEESTAAAHLLSEETERLAHMIGRFKTGQEA
                     IHEAESHAGKKTSPLASRPMLKTVSSTGNTAVLRKPSPSKTEWRDF"
     gene            complement(8797..9237)
                     /locus_tag="VGB93_00135"
                     /pseudo
     CDS             complement(8797..9237)
                     /locus_tag="VGB93_00135"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
BASE COUNT         1849 a         2801 c         2856 g         2077 t
ORIGIN      
        1 cgtcctgatg atgaaggaca acatcatcac gatcgactgg ggagatgagg ttctggcgaa
       61 gacagtcctc acccatgccg gtgaactcaa gattggcttg gcgaaacccg acagcgttcc
      121 tggcggcgcc aacaaggatg cagcaaccaa ggcagccgcg aaagtcgcgt gagccgaagc
      181 aggagatcgc gatggacttc cagatagcta ttaccggctt cgtcgcactt tacatcttca
      241 tgctcgccgc ctttgcgggt tgggtgatca tcggccacgt accggcaatc ctgcatacgc
      301 cgctgatgtc gggctccaac ttcgtccacg gcattgtcgt ggttggcggc ctcttcgctc
      361 tgctcaacgc gaccaatgtc acgcaacagg cgatcggatt cttcgcggtg ctgctcggcg
      421 ccggcaacgc ggcgggcggg tatgcagtta cccatcgcat gctcgcgatg tttcagacga
      481 gcggacaaca gaccaaaccc gccaagtcca gccatcacgc caagtcgagt cacaccaagt
      541 cagggcatcc gaagacgcac cacgcaaaga aagcttgagg aaccgatatg ctgaccgtga
      601 ttccccaact gaccattggc gtcgtcgacc tcgtggcggc ttttcttttt atgttcgggc
      661 tgaagcgcat gtcgtcgccg gtgacggcgc cttcggggat cttcgtcgct ggcctgggca
      721 tgctcgtcgc cattctggcg agctttcttt atgcgtttga cgtcagtgct gcggcgaggc
      781 cgcatttgtt ggtcaatatc gggcttgccg ccgtcgcgct tgtcgtgggt ggtggtattg
      841 cttggtggag cggccggaag gtcgcgatga ccgcaatgcc gcagatggtg gcgctctata
      901 acggcatggg cggtggggcc gccggggcca tcgccgcggt cgaattgttc ggggataaaa
      961 ctcacggcgc aacccaactc gtcgtcacat tgcttggcgc gttgatcggc gctgtatcgc
     1021 tctccggttc gctgatcgcc tgggctaagc tcgatggcgt tatcggcaag ccgctcagag
     1081 tcggcggcca gcaagccatc aacggcgtcg tcatgctggc gacgctcgcg gtcggcggat
     1141 acctcatctt cgtagtcctg ggcggcgcgg ttccgctcat tccactgccg gcgctgatcg
     1201 gactgttctt cgggctggcg ctggtcttcg gcgtcctgat gacgctgccg atcggcggcg
     1261 cggatatgcc ggtggtcatt tctgtttaca acgcctttac cggtcttgcg gtcgggctcg
     1321 aaggtttcgt cctgcagaac ccggccctga tgatcgccgg catggtggtc ggcgccgccg
     1381 gcatgctcct caccttgctc atggcgacgg ccatgaatcg gtcagtcgcc aatgtcctgt
     1441 tcacgaattt cggcgatgtg ccgaagcacg cgcaagggga catcaaaggc agcctgaagc
     1501 cggtcgaagc cagcgatgcc ggcatcttta tgcgctatgc cagcaaagtc atcatcgtgc
     1561 cgggttatgg cctcgcgatg gcgcaaggcc agcagaagct ctacgagttc gtcaagctgc
     1621 tgcaggccgg gggcgtcaca gtcaagttcg cgatccatcc ggtggccggg cgcatgcccg
     1681 gccagatgga cgtcctgctg gctgaagccg gcgtgcccta cgatctcatt tttcagctcg
     1741 aagacatcaa cgatgacttt gccagtgctg acgtcgcgct ggtcatcggt gccaatgacg
     1801 tcgtgaaccc tgcggcgcgc accgacaaat cctcgccgat cttcggcatg ccgatcctca
     1861 acgccgacaa agccaagaag gtctacgtcg tgaaacgcgg tgagggcaaa ggctatgccg
     1921 gcatccagaa cgcgctgttc tacggcgata attgcgacat ggtctacggc gacgcccagg
     1981 ccgtgctggt aaaaatgatc gaggcggtga gagggcttgg gttgctcgcc gcagcttagc
     2041 ccacacccaa gccggcacga ttgatcccaa ctgaaaacag gagtgatcca tgacgcgtaa
     2101 gatgcaagcc gccatcgtcg agcagttcgg aaagcccctc gtatttcagg agtgggatat
     2161 ccccactccg gggccgggtc agatcctcgt caaaaccgaa gcctgcggcg tctgccatac
     2221 cgatctccat gccgccagtg gcgactggcc cttgaagccc aagcttccct tcatccctgg
     2281 tcatgagggc atcggactgg tcgtcgccct cggatcgggc gtaacgatcg tcaaggaggg
     2341 agaccgcgtc ggcgtgcctt ggctctattc ggcgtgtggt cattgcgaat attgcctgtc
     2401 cgcttgggag acggtatgcg gggaagcgca gttcggcggc tatacgaaaa acggtggttt
     2461 cgctgaatac attctcgccg atcccaatta tgtcgcgcat gtgcctgccg gtcttggcgc
     2521 gcaagaagcc gcacccctca tctgcgctgg catcaccacc tacaagggca tcaaggagac
     2581 caaggccagg gcgggcgagt ggatcgccat ctccggcgcc ggcggccttg ggcaccttgc
     2641 catccaatat gccaaggtga tgggattcca ggtctgcgcg gtcgacattg acgacggcaa
     2701 gctcgcccac gccaagaagc tgggtgcgga tgtcgtggtc aatgcgaaac acggtgaccc
     2761 ggccgccgcg gtgaaaaggg caaccgacgg cggcgcgcat ggcgtactga tcacggcccc
     2821 ctcgctccct gcgttcaagc agggcgtcgg catgacgcgc aaacgcggca cctgcgttct
     2881 ggtcggcctg ccgccgggcg attttccgct acctctcttc gatgtggtgg cgaattgcat
     2941 caccgtccgc ggctcattcg tgggcaaccg cgaggacatg gccgaggcgc tccacttcgg
     3001 ggcccaaggc aaggtcaagg ccgacatcga gttgcagccg ctatcggcga taaacgacgt
     3061 cttcaatcgg ctcgagcatg gtgatgtgcc gtcgcgcgtg gtcctcgatt tcgtcggggc
     3121 ggaggcgctt ggtcatcagt cgaaggccgc cgcggggaag acgagggaaa cagcaaacat
     3181 cgggtaagaa agctgaccga tgtgaaatcc accgtcgtta tggcggaggc ggagagcccg
     3241 ataaacgggc tcgagcatga tgcgatgtct cgcatcggct tgaatacttg gagcgggatc
     3301 aatggtgagc catcgcatag cgattccatc ttaacacatc ccactccaga ccccgtcgct
     3361 ccggttcgcc acatagccaa agccgatcgt ccgacaattg ccgccggcac accagccatc
     3421 gatatgatgg aggtgccgca gagcccggtg atgacctgcg atgaatgaga cgtgagcccg
     3481 acctgcaccg atcccgaccg tccgatgcga atcgaggatt acggccttgt cggcgattgc
     3541 aagactgcgg ccctcgtcgg gtggaacggc tcggtcgact ggctctgctg gccaagattc
     3601 gatggcgcgg cctgtttcgc ggcgcttctt ggcagcgcga agcacggacg ctggtcgatt
     3661 gtcccggccg atgggtcgtg tagcacttcg cgttcctatc ggggcgacac aatggtgctg
     3721 gaaactttgt tcgagacggc ggatggcgct ttcgcgatca tcgatttcat gcccgtcgat
     3781 caaccacatt catcgcttat ccgcattgtc gaaggccgtc gcggccgcgt gccagtgcgc
     3841 atgaacctca ctctgagatt cgactacggc tcgtccatcc cctgggtcac gcggcttcct
     3901 gaaagcgatg gaatcgtcgc catcgcaggt ccgagcctag cggtcctgcg aacgcccgtc
     3961 gcgctacgcg gcgaagatct gtcgacttcg gccgatttca gcgtcgatgt tggcgagcgc
     4021 gtgccttttg ttttgactta tggcgaatct catcggccgc ctccgctccc ggtcgatgtc
     4081 gatgcagcgt tgcagcagac ggagaagttc tggcgcgatt gggcggcgcg atgttcctat
     4141 aaggggcagt ggcgggcggc tgttctgcgc tcactgctga cgctgaaggc gcagaccttt
     4201 gccgagaccg gagggatcat cgcggcgccg acgacgtcat tgccggaaca gcttggtggt
     4261 cagcgcaatt gggactatcg cttctgctgg ctccgggacg ccacgttgac attggtcgcg
     4321 ctgatgggcg ccggctacta cgacgaggcg aaggcatggc gcgattggct gcgccgcgcg
     4381 gtggccggca caccgagcga cgtgcagatc atgtatggcg ttgccggcga aaggcgactc
     4441 ttcgaaaggg aaattccctg gcttcccggc tatcagggcg cggcgccggt gcggatcggc
     4501 aacgccgcct ccgagcaact ccagctcgac gtcttcggcg agatcatgga tgcgcttcat
     4561 gtggcgcggg aaatcggctt tgcccatttg tcgactgact ggagcctgca atgctgcgcg
     4621 ctggagcatt tgacggccat ctggcgacag ccggatgacg gtatctggga gatgcggggc
     4681 ggccgcaggc acttcaccca ttcgaagatc atggcttggg tcgcattcga tcggtcgatc
     4741 agggatgccg aaaaatatgg ctttgatgca ccgctcgtac actggcgcgc ggagcgcgac
     4801 gagatccgtc gcgtggtttg cgaacagggc tatgatcgaa agcgcggaac cttcactcag
     4861 agtttcggta gtccggaact cgacgcgagt ctgctgctga tctcggatgt cggtttcctg
     4921 cccatcgatg atccgcgcgt ggccgggacg atcgccgccg ttgaacgcga gttgtgcgtc
     4981 gacggcttcg tgctccgcta tcggacggag agcggcgccg acggcctgcc gtccggcgaa
     5041 ggcgttttcc ttccttgcag tttctggctc atcgacgtct accagaagca aggcaggaat
     5101 gccgaggcgc atgcgatgct cgatcgcctg ctcgctctgc gaaatgatct tgggcttctg
     5161 agcgaagaat acgacgtgat agcagcgcgg caggtgggca actttccaca ggccttctcg
     5221 catctctcgc tggtacgcac ggttctcagc ctggatcggc gaaagccgat caaaaatcgg
     5281 cctgggatac cgacactcga cgaaagtgta aaaagatagg cgcgacactg caccggcttg
     5341 cgaagcgtca cgacgtattg cgcaacttga tccgcgtcct cgcgtggcga atgagtcatt
     5401 agagttgctg aggtgcggcc gaagtaccaa gctcttcggc cggcagaacc ggcgtgtttg
     5461 caatgcaccc accgagtggg tgtcaatttt gctcagacgc acctgatcgg ctcgtgcatg
     5521 ccatgtcaga tgaaaagagc caccttttct ataccttcgc tatcgacgat aatccgagct
     5581 gcccacaatg cgcaatgccc atgcgcatta cggcgaacag gccgagcacc cttctcggcg
     5641 aagcctacga atgtcagaca ttcacttgca aattcgggtt tgaaatcgag cgcgacacgg
     5701 gccgcgcatt cgcgcgcatg accgcaaaag ccacgcataa atccaaagcg ttcaaaccga
     5761 ataagaccag gcggacgcgc agcagttcct gatctgctgc ggaaaatatt tccgaaaagc
     5821 gttcagtttc gcacagtctc caggctatcg gcattggatg gttctgcgcc gcgagtcgaa
     5881 ggctttttcg tatgtcgata aaacttacaa tcgcggtggc tcgacacgtc cccccaaatg
     5941 gtcgtcgaga ggagaggccg cctgattggc ggcctctttt caattgaggc gcctgatgag
     6001 acaattaatg cccatattta aactttacgc ccggcaaccg atcgcgtttg gcgcttacgc
     6061 tcgactgccg tgttcgaaat gtagggatac tatgagcgtc actcgtcgag gcccgagcga
     6121 atacggcgga ctctatgaac ggcaactgct cgcatgccga agctgcggcc acgaaatgtc
     6181 gcgcgtcgta aaccgccgag gagaaccggt gcactgatcg ccccgtgggg tcgcttttct
     6241 attgccgcgt cacgcaacac cttccacgtt gagacgttct tgcgacatat caattacagg
     6301 cgcccatgcg cagttttaag ctggctgcgg tggtccggcg gtggagtatc tcaatgtcgc
     6361 aagaatcgct cgccttgacg gtcgccgtcc tcgtaactga tggttttgaa caagtcgaac
     6421 ttaccgaacc gcgcgccgcg cttgataaag ccggcgcgaa gaccctgatc gtttctccca
     6481 cggatgttgt ttttcgctat ctgttcttga gcctctcgtg atctcaagcg ttcgcacacg
     6541 atggcggtag atcgcgccta cctcgctgat tgatactcgc gcgtgtttgc ctgagcgcgc
     6601 cagttgttct aggggcgcga tgtcaggaat ttcaacgcaa aaccgatttc gaaaacgcgg
     6661 atgacatccg ccgcgcggtg gatcgccccg cgcaagacga tgcggctgcg ttcgggcgga
     6721 atgatcctcg ccctgatcag agcatcgtcc tctgagatat tgacgagcgt gccttcgcgt
     6781 aacgtgccgg tgtgatcgag gaattggcat cttttaatcg acggctcgac cgtcaagatt
     6841 tttgaggtcg cgccggcgcc acgttcgacc gcgactgcat agttcgcggc catgcgtgcc
     6901 agcctgtcgc ggacggccgg ccgatattcg ctatcgatcc gcacgtcaaa gccttcccgc
     6961 ttcttggcgg cgatagtgcc ggcgaggctg cctaattcct ctacgtgtag acgcacgccg
     7021 gtgccttccg acaagctttc agcctgttta cgcgacgctt cgatagattg gccagccttc
     7081 gtatcgtaga cgagatgaac ggcatcggcc gcgatcctcg tcgttttgca gtgtaacggg
     7141 gcgcctgtag cgctctcgca ggtcgtctgc aattgcgcgt tgatcgatgg atgatagttg
     7201 taaagagcca tcatggtagt cagctcttcg gcctgaatcg ccggagagcg ttcgcgactc
     7261 tgcagcactc ttttctcagc cgcgatcaca gaccgcggct cggagtccga tgcccgttcc
     7321 ttccctaatt cccaaattct gtaacgatgc ttgtcgcggt cgttcattgg gtcttcctcg
     7381 cttcgagcgg aggtaccaaa tgtaacagat cagattattt cccaagaaat agtaaagtcg
     7441 acttccctcg aggtcgcaca agcgtccgga tacggagaat ggggcgcata cagtcgtgaa
     7501 ggcgagcgac aaccgtagca ccgcacgaat ttacggctac caatgcaacg aaacagagtg
     7561 ccgtaagaat gtcgcaccgg ccattcgatt tttgtctgat attgaattcg catctctcgt
     7621 ttgggaacat cgagaccggc ctcaaaaatc gcgccactcc gttttagacg gcgatggttt
     7681 gcgaagcacg gccgtattgc cggtgctgct gaccgttttc aacatcggcc gcgaggccag
     7741 cggacttgtc ttttttcccg catgagactc agcttcgtga attgcctcct gacccgtctt
     7801 gaaacggccg atcatatgcg ccagacgctc ggtctcttcc gaaagcaaat gcgctgccgc
     7861 ggtcgactcc tccaccatgc cggcattctg ctgggtgacc ttatccattt cgttaaccgc
     7921 gatattgacc tggtgcagcc ccgccgcctg ttcctgcgca ctcgtcgcga tattgctgac
     7981 gacctggttg atttcgatta cctgctcgac gatccgctga agcgctttgc cggtctcctc
     8041 gaccaaatgc acgccctgat cgacctgggc agttgaggcc gaaatcaatc ctttgatttc
     8101 cttggcggcc tctgcggatc gttgcgctaa cccgcgcact tccatcgcga cgacggcaaa
     8161 accgcggccg gcgtcgcccg cgcgggcggc ctctacgccg gcgttcaagg cgagaaggtt
     8221 cgtttggaag gcaatctcgt cgatgacgct gatgatctga ctgatctggt gggatgattt
     8281 ctcgatgcca gacatggcgt gcatcgcttc gcccactatc acgccggctt tttccgcctc
     8341 catctttgcc gcggagacgg taacatgggc gtagtttgcg ttctcggccg tctttttgac
     8401 cgtcgtggtt atttcgtcga gggcggcagc cgtctcttcc aggctggccg cttgtctctc
     8461 ggtacggtgt gacaggtcgt cggcggcgac agcaatctgc tcggtgcccg agcgaatggc
     8521 ctgcgcattg gcgctgaccg ccaacacggt ttgctgcagc ttctccatcg agctgttgaa
     8581 gtcgatgcgt agttgctggt attcgccggg aatgtcgtcg gccaatcggc aggtcagatc
     8641 gccgtccgag agagaagaca tggctttgcc gacggaagcc gacacatcct cgcgttcttg
     8701 cgcgagcacg gcttcttccg ccctcttacg cgcggcttcg gccgctctga gataagccga
     8761 aatcgcaagg tccatgtcga gcatagttgc cttgacgagt gcgccgagtt ccgccgcgac
     8821 ctgttggacg gggacgcgcc gcgctccgaa accgcgcttt tggaaacgcg cctccataac
     8881 tttcgcaacg agcacctcga tcacgagagc gtagccgccg atgtaccagc gcggctcgag
     8941 gccgatcttg acgtgaatat cgccgacggc cgaggcggcc ttgacatagt cgacatcaaa
     9001 ccggccgcct gatatcgcgt cccagtgagc aagctgtctg cttttagcgc tcacaatctg
     9061 ggcttcgttt gagaagaatt tgaccgtctc ggggaagcgg cgcacttgcg cgtaaaatgt
     9121 atcgagcgcg ccaggcaatt cctgggtgat gatggttttg accgcctgaa tattcttctg
     9181 ggcgtcggcc ccaaggttca tgaatttgag ttggtcggct agcacgtcca ctgacatcgc
     9241 ttggctccaa aaagggcaat ccaaccgccg cggagtctta gaatctactg tgtcgtttcg
     9301 cgtaacgcgt atgcttttta ctaactaaca aagatcggcc gcgtggcctg cgaatcacca
     9361 aataaaataa gacctacttt tggtaagttg atgcgacgga atggtccatg aaaatttcac
     9421 atttatgcca ttttttaaca aaacatcttc aataatttag gtttatatac ttagattggc
     9481 aacatagatt gtgaacatat attgtgcgtg attagatatg tgatcaaata ggaccagcgt
     9541 tgccaattca tattatctat gggcttatcg tagcggcgca ggt
//
LOCUS       DASWOG010000008         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_330235,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000008 DASWOG010000000
VERSION     DASWOG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330235"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..965)
                     /locus_tag="VGB93_00140"
     CDS             complement(258..965)
                     /locus_tag="VGB93_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0144569.1"
                     /translation="MAADLPSSKGPPVYAPPPPVFSWTGVYIGGQVGYEWGRTTFATV
                     NPVTGVIAGGFPAIDEQGVVGGGHVGYNYQLGGLGLGFGGFLVGIEGDVNGASYTGSE
                     LLGVTTLRERTPVDASIRGRVGITFDRFLVYGTDGVAFGSIRESLTTPIFSGSATTGR
                     VGWTVGGGLEYAIDNNWSVRAEYRYTDYGRFNESFATVRLPVTLRYHPTDNRVQVGFS
                     YKFDEAPPPAPPVVAKY"
     gene            complement(1090..1464)
                     /locus_tag="VGB93_00145"
     CDS             complement(1090..1464)
                     /locus_tag="VGB93_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0144570.1"
                     /translation="MAGMLYLPRLFVYHVGAEKGSQQSETFKVMERRLEVAIMRPALI
                     IVYVTGITLAVKGALFKAAWLNWKLLLVLAMTGIYFYLAALRFRFAADRNAHSAKFYR
                     VLNEVPTLLLIGIVVLAVVKPG"
     gene            complement(1522..2571)
                     /gene="hemE"
                     /locus_tag="VGB93_00150"
     CDS             complement(1522..2571)
                     /gene="hemE"
                     /locus_tag="VGB93_00150"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0144571.1"
                     /translation="MAASTLAADKPLLRVLNGEVLPSTPVWLMRQAGRYLTEYKQTRL
                     EARSFLDLCYNPKLAAEVTLQPIRRFHFDAAIIFSDILVVPDAFGQKVDFESGEGPRL
                     QPITSADDLKALRETIDLDHLSPVFEAVHLVKQALPERTALIGFCGAPWTVASYMIAG
                     RGTPDQAPARLFAYRNPDIFAALIERLIEGSIAYLSRQIEAGADCIQIFDSWAGALAP
                     AEFERWALDPVRRIIAALRARHPRTKIIAFPRGAGLALAGYAAATGADALGLDTSVDP
                     ATAAAVIPPNVVLQGNLDPLALIAGGKPLSEAVAKIQAGFRDRPHIFNLGHGILPETP
                     LANVEALLALLRPEA"
     gene            2934..3776
                     /locus_tag="VGB93_00155"
     CDS             2934..3776
                     /locus_tag="VGB93_00155"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY0144572.1"
                     /translation="MARNYFHLHLISDATGETLIAVSRAVVAQYDAVESIEHLYPFVR
                     SPMQIEKAIDEIDEAPGVVLFTLIDKDLTRRLVSACHELNVPCHSVLQPIADLFRAYL
                     GSPSAARPGDQHTLNAYYFNRVEALNYTMLHDDGQMTDDYDAADVLLLGVSRTSKTPT
                     SIYLANRGIKTANIPIVPNVPLPKAIFSLQKPLIVGLITSADRLVAVRQQRLDASLTR
                     FDSTYVDPVAVSEEVVQSRRLFSHYGWPVIDVTRRSIEETAAAIISLYKTHRAKLGLT
                     EQPR"
     gene            3773..>3913
                     /locus_tag="VGB93_00160"
     CDS             3773..>3913
                     /locus_tag="VGB93_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0144573.1"
                     /translation="MSGLWLAKTPLVLASESMARQALLKAAGIPFDVIAAEIDERSVE
                     APL"
BASE COUNT          857 a         1195 c         1110 g          751 t
ORIGIN      
        1 cagaggactg cgcagacgct cgacatcagc agggcgcgaa tcatctgtcc caagggcctc
       61 aaaattctaa gattgcaaag gtgatcatta gagcaaatcc ctgaccacac aacgctcgcg
      121 gcgccaccgg gcgcggtttc cgtgtcgctg tggccagcgg gtgacaattc tggcccagtc
      181 tatccggcga cccttaggga ataagaaaac cggcgggttt ttgcccgccg gttcgttaaa
      241 gtcagaatcg caaagtctca gtattttgcc acgacgggcg gagccggcgg cggagcttcg
      301 tcgaacttat agctgaagcc gacctgcacg cgattatccg tcggatgata gcgcagcgtc
      361 actggcaaac gcaccgtcgc aaagctctcg ttaaaccgtc catagtcggt atagcggtac
      421 tcggcgcgca ccgaccaatt gttgtcgatc gcatattcaa ggccaccacc gaccgtccag
      481 ccgacacgac ccgtcgtcgc gctaccgcta aagatcggcg tcgtcagact ctcgcggatc
      541 gagccgaagg cgacaccgtc ggtgccataa accaggaaac gatcaaaggt gatgccgacc
      601 cggccgcgga tggaagcatc aacgggggtg cgctcgcgca gcgtcgttac gccgagaagc
      661 tcgctccccg tgtagctcgc gccattcacg tcgccttcga tgccgacgag gaacccaccg
      721 aagccaagcc caagcccgcc aagctgataa ttgtagccga cgtggccgcc accgacgacc
      781 ccctgttcgt caatggccgg gaatccaccc gcgatgacgc ccgtgaccgg gttgactgta
      841 gcaaacgttg tccgacccca ttcgtagccg acctgcccac cgatatagac gcccgtccac
      901 gaaaagaccg gcggcggagg cgcgtaaacc ggagggccct tgctgcttgg aagatcggcc
      961 gccatggcga cagaccctaa aacgagcgac gaaacacctg ctaaaatcac tgcacgaatc
     1021 aaaggggcac aacgcgtttc gcgcgggcgc ggccaaaatc atccaaagga agcccggtgt
     1081 gagcgaccgc taacccggct tcacaaccgc cagaacgacg atgccgatca agagcaacgt
     1141 cggaacctca ttcaaaaccc gatagaattt tgccgaatgg gcgttgcgat cggcagcgaa
     1201 gcgaaaccgc agcgcggcca gataaaaata gatgccggtc atcgcgagga cgagcagcag
     1261 cttccaattt agccacgcag ctttgaacaa cgcgccctta acggcaagcg taatccccgt
     1321 cacataaacg atgataaggg ccggccgcat gatcgcgacc tcaagccgcc gctccatcac
     1381 cttgaaggtt tccgactgct gcgaaccctt ctcagccccc acgtgataga cgaagagccg
     1441 cggcaaatag agcatgcccg ccatgaacgc gatgatcgcg atgacgtgca gagctttgat
     1501 ccaaggatag atttggtcca ctcacgcctc cggccgcaac agcgccaata gagcctcgac
     1561 attggccaag ggcgtttctg gcagaatgcc gtggccgaga ttgaaaatat gcggccggtc
     1621 gcgaaagccg gcctggattt tggccacagc ctcgctgagc ggcttaccgc cggcgattag
     1681 cgcgagcgga tcgagattac cttgtagcac gacgttcggc ggaatgaccg ccgccgctgt
     1741 agccggatcg accgacgtat cgagacccaa agcgtctgcg cccgttgccg ccgcatagcc
     1801 tgctagtgcg agacccgcgc cgcgcggaaa ggctatgatc ttcgtccgcg gatggcgcgc
     1861 ccgcagcgcc gcgatgatcc gtctgacggg gtccagcgcc caacgctcga attccgccgg
     1921 ggcaagagcc cctgcccaac tgtcgaaaat ctggatgcaa tccgcgcctg cttcgatttg
     1981 gcgcgacaga taggcgatcg agccttcgat aagccgctcg atcaacgccg cgaaaatatc
     2041 cgggttgcga taggcgaaga gccgcgctgg ggcctggtcg ggcgtcccac gcccggcgat
     2101 catgtagctc gcaaccgtcc acggcgcgcc gcagaagccg atgagcgccg tcctctccgg
     2161 caaagcctgt ttgaccagat ggaccgcctc gaagaccggc gacagatgat cgagatcgat
     2221 cgtctcccgc aaagccttca gatcgtcggc actcgtgatt ggttggagcc gcggtccttc
     2281 tccggattcg aaatctacct tttgaccgaa ggcatcggga acgacgagaa tgtcgctgaa
     2341 gataatcgcg gcatcgaagt gaaaccggcg gatcggctgc aaggtcacct cggccgccaa
     2401 ttttggattg tagcagagat cgagaaacga tcgcgcctcg agccgcgttt gtttgtactc
     2461 ggtcagatag cgacctgctt ggcgcattag ccagacgggc gttgacggca aaacctcgcc
     2521 gttgagcacg cgcagcaagg gtttgtcagc cgccaaggtc gaagccgcca atataaaaat
     2581 ccttatctaa agactcttct attatctctc ttattagggg gtccgattag gctcgtttcg
     2641 gatttttcac aatcgaaccg gggcattttg ttttatgctc gattcccaca ggcaggcggc
     2701 tgctgggaaa ggttaacgat tcattaatca cgccaacggc ttgcctgttt caattcggta
     2761 atctttcaac tcttattaaa ttatcgacag aaccagcggg agcccgacga ttatctgcgc
     2821 agcgtcagcg ctgttgacaa agaagccgcc gctgcgggac atgtccccaa gagggcggcg
     2881 gcggcggtga cttatcccca ccggtccaca gggcctcgcg acggatgcgg cttgtggcgc
     2941 gtaactattt ccatttgcat ctgatttccg acgcgaccgg tgaaaccttg atcgccgtga
     3001 gccgggcggt agtcgcgcaa tacgatgccg tggaatcgat cgagcatctc tatccgttcg
     3061 tacgctcgcc gatgcagatc gagaaggcga tcgacgaaat cgatgaggcc ccgggcgttg
     3121 ttcttttcac gttgatcgac aaggacctga ctcgtcgtct cgtctcagct tgtcatgaac
     3181 tgaacgtgcc gtgccattcg gtgctgcagc cgatcgccga tctttttcgg gcctatctcg
     3241 gctcgccgag cgccgcgcgg ccaggcgacc aacacacgct aaatgcctat tatttcaacc
     3301 gcgtcgaagc gttgaattat acgatgctgc atgacgatgg ccagatgact gacgattatg
     3361 acgccgctga cgttctgctg ctcggcgtca gccgaacgtc aaagacgccg acgagcatct
     3421 atctcgccaa tcgcggcatc aagaccgcca atatcccgat cgtgcccaat gtcccattgc
     3481 cgaaggcaat cttcagtctg caaaaaccgt tgatcgtcgg ccttattacc tcggccgacc
     3541 ggcttgtcgc tgttcgtcag caacgtctcg atgcgtcgct gacacggttt gattccacct
     3601 atgtggatcc tgttgccgtg agtgaggaag tcgtgcagtc gcggcgtctc ttttctcact
     3661 atggctggcc ggtcatcgac gttacgcggc gctcgatcga agagaccgct gcagccatca
     3721 tctcccttta taagacgcat cgcgccaaac tcgggctcac ggagcagccg cgatgagcgg
     3781 gctttggttg gcaaaaaccc ccttagtgct tgcctcggag agcatggccc gacaggcgtt
     3841 gttaaaggcc gcggggattc ctttcgatgt catcgccgcc gagatcgacg aacgtagcgt
     3901 cgaggcgccg ttg
//
LOCUS       DASWOG010000009         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_55317,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000009 DASWOG010000000
VERSION     DASWOG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55317"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGB93_00165"
     CDS             complement(<1..836)
                     /locus_tag="VGB93_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0144574.1"
                     /translation="MIDVQQLECFCAVVRSGSFSKAAAAMSIGQPGLSRHVRRLEEQL
                     GSKLLYRNGRGVALTPAGSQFHATVSVLITQLKEAYDTAAQDSDAPAGHVTLGLPTSM
                     AGLVGAPVLRALKARYPHIELSLIDGFSGHIHEWLVSGRVDMAILHDARHAPSIAVEP
                     LSSEDLFLIGPKTPPGALVRGNETIIALRDIATLPLAIPGPDHGLRRQIDRVSAEAGV
                     KLSVESDVDSLTVIKELIKDGGIYTILPIGCVYQETLAGTLRVCRIVDPPIVNTFVLA
                     AA"
     gene            complement(897..1427)
                     /locus_tag="VGB93_00170"
     CDS             complement(897..1427)
                     /locus_tag="VGB93_00170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0144575.1"
                     /translation="MTSQSASSFTAREFRDALGLFPTGVAVVTALSGAGEKLGVTVSS
                     FSSVSLDPPLVSFGLAKSAHSFDDWVATPHFAINVLAEEQEHLSTKFARALSDKWSGL
                     SPRQGAAAEVPLLDEALAWFECAKFAQHDAGDHLIVLGRILSFARTPHEGRRPLVFFR
                     SAYHRLHGSESVKPAE"
     gene            complement(1432..>1728)
                     /locus_tag="VGB93_00175"
     CDS             complement(1432..>1728)
                     /locus_tag="VGB93_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0144576.1"
                     /translation="ELSGAAMITLPSSVKDLESPKTAKDMERFMQSADTGARERVQLM
                     RMAWDFIGSEFGNRSAQYEKFYGGASFLVKQNMFRAYDFSRATELVDTALALPD"
BASE COUNT          382 a          545 c          497 g          304 t
ORIGIN      
        1 tgcgctgctg ccagaacaaa cgtattgacg atcggcggat cgacaatgcg gcagacacgc
       61 agcgtaccgg ccagcgtttc ctgatacacg cagccgatcg gcaggatcgt gtaaatgcca
      121 ccgtccttga tcagctcttt aatgaccgtc agcgaatcga cgtcgctttc cacgctcaat
      181 ttcacgccgg cctccgcact gacgcgatcg atttgccggc gcaggccgtg atctggacca
      241 gggatcgcga gcggcagagt cgcgatatcg cgcagcgcaa ttatggtttc gttgccgcgc
      301 acgagcgcgc ccggcggagt cttcggcccg atgaggaaca ggtcctccga cgagagcggc
      361 tcaaccgcga tcgacggcgc atggcgcgca tcgtgtaaaa tcgccatatc gacgcggccg
      421 gacacaagcc actcgtggat atgcccggag aagccatcga tcaggctaag ctcgatatgg
      481 ggatagcgtg cctttaaggc gcgtaacacc ggagcgccga ccagtccggc catcgaggtc
      541 ggcaggccga gcgtcacatg gccggcaggc gcgtcgctat cttgcgccgc cgtgtcatag
      601 gcctctttaa gctgagtgat gagaaccgac acggtcgcgt gaaattggct cccagccggc
      661 gtcagcgcaa caccgcgacc gttgcgataa agcaatttcg agccgagctg ttcttcgagg
      721 cggcgcacat ggcggctcag acccggctgg cctatcgaca tcgccgcggc cgccttggaa
      781 aaactgccgc tgcggacgac ggcgcaaaag cactcaagct gctgaacgtc tatcattttc
      841 ggcactcaca catattcgca accgacgcga aatacgtgcc aagtcccgga ggatgcctat
      901 tccgctggct tcacagattc ggacccgtgc agccgatgat aagcgctcct gaaaaagacg
      961 agcggccggc gcccttcgtg cggcgtccgc gcgaaagata atatgcgccc gagaacaatc
     1021 agatgatcgc cggcatcgtg ctgcgcaaac ttggcgcatt caaaccacgc caatgcctcg
     1081 tcgagcagcg gcacttcggc cgcagctccc tgccgtggcg aaaggccact ccatttgtcg
     1141 ctcagagcac gcgcgaattt cgtcgataga tgttcctgct cttcggcaag cacattgata
     1201 gcgaaatgcg gcgtagccac ccaatcgtca aatgaatgcg ccgactttgc cagcccgaaa
     1261 gagacaagcg gcggatcgag cgaaacggac gagaacgaac ttaccgtcac gccaagcttt
     1321 tcccccgcgc cgctcaacgc cgtgacaacc gcgacgcccg tcgggaaaag accgagcgca
     1381 tcacggaatt cgcgcgctgt gaaagaggaa gccgactgcg aagtcataac cttagtccgg
     1441 caacgcgagc gcagtatcga cgagctccgt cgcgcgcgag aaatcataag cgcggaacat
     1501 gttctgcttc accaggaacg acgcgccacc gtagaacttc tcatattgtg ccgagcggtt
     1561 gccaaattcc gaaccgatga aatcccacgc catccgcatg agctggacgc gctcacgcgc
     1621 accggtatcg gcgctttgca tgaagcgctc catgtccttt gcggtcttgg ggctttcgag
     1681 atccttgacc gaggacggca atgtgatcat cgccgcgccg ctcaattc
//
LOCUS       DASWOG010000010         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_238607,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000010 DASWOG010000000
VERSION     DASWOG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238607"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VGB93_00180"
     CDS             complement(<1..331)
                     /locus_tag="VGB93_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0144577.1"
                     /translation="MTDSVYLDLGRRSRRLRVDTLVKLRWLAVFGQLAAVLLVRFGLG
                     FPIPLIPLTICLLFIAGSAWLNVVLGFRYSQSDRLEDKPAAAILGYDIIQLGALPYLT
                     GGIENPFA"
     gene            635..>1563
                     /gene="phaZ"
                     /locus_tag="VGB93_00185"
     CDS             635..>1563
                     /gene="phaZ"
                     /locus_tag="VGB93_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY0144578.1"
                     /translation="MNDSYSYEWHELAYLALAPARAASDIARVWFKSPLNPLSHTPVG
                     RNVAASAELFERLTRRYGKPVFGLDETIVDGRTVPIIEKIVWERTFCRLLHFERQWPQ
                     AHAPQAKLLIVAPMSGHYATLLRGTVEAFLPFCDVYITDWVDARMVPIARGGFDLADY
                     IDYLIDIFRFLSAKKDGLPLHTIGVCQPAVPLLAAVAHLEKQHDPDVPASMVLMGGPI
                     DTRRSPTAVNKMAESRGSKWFKDHCVHPVPYPYPGARRLVYPGFLQLSGFMAMNLDRH
                     VNAHIEMFNHLMEGDGDSAEKHREFYDEYLAVM"
BASE COUNT          313 a          478 c          465 g          307 t
ORIGIN      
        1 ctgcaaatgg gttctcaatc ccccccgtga gatagggcaa cgccccaagc tggatgatgt
       61 catagccgag aatggcggcg gctggcttgt cctcaagccg atcgctctga ctgtaacgga
      121 agcccagcac gacgttgagc caggccgagc ccgcaatgaa caggaggcat atcgtcaggg
      181 gaatgagcgg gatcggaaaa ccgagaccga agcggaccaa cagcaccgcc gcgagctggc
      241 cgaagacggc aagccagcga agcttgacga gcgtgtcgac gcgcagccgg cgcgagcgcc
      301 ggccaaggtc aagataaacg ctgtccgtca tacttatccc ttgcccgatg taccggtttt
      361 cgtttcccgg tgaaaccgga cggcggcatt atagcgggct gccgagccaa acgccgagaa
      421 aaatcaaggc ggggcgttgt ccggcgtgca acgcctcggc gaggcctcta cttttcgccc
      481 agctgcaact tcgcgcggtc tgcggcattt agctgtagct ccgctcaatc tccggattgt
      541 attgcggtcg ggcagaaacc ataatagcaa tgctgcgttg caacataaat cgcgcgggtc
      601 atgagccatc gcggtattag gaatgaccag ggcgatgaac gattcctatt cttacgaatg
      661 gcatgagctg gcctatctgg cgctggcgcc ggcccgtgcc gcctcggaca tcgctcgcgt
      721 ctggttcaaa agcccgctca atccgctctc gcatacgccg gttggccgca atgtcgccgc
      781 ctcggccgag cttttcgagc ggctgacacg gcgttatgga aagccggtct tcggtctgga
      841 tgaaacaatc gttgatggcc ggacggtgcc gattatcgag aagatcgtat gggaacggac
      901 attctgccgc cttctccact tcgagcggca atggccgcag gcccacgcgc cgcaagccaa
      961 acttctgatc gtcgcgccga tgtcaggcca ttacgccacg ctgctacgcg gcacggtcga
     1021 ggcgtttctg ccgttctgcg atgtctacat cactgattgg gtcgatgcgc ggatggtgcc
     1081 gatcgcccgc ggcggcttcg atcttgccga ttacatcgat tatctgatcg atattttccg
     1141 cttcctcagc gccaaaaagg acggcctgcc gctgcatacg atcggcgtct gccagccggc
     1201 cgtgccgttg ctggccgctg ttgcgcatct cgaaaaacag cacgatcccg atgtgccggc
     1261 ctcgatggtc ttgatgggtg gcccgatcga cacgcgccgc agcccgaccg ctgtaaacaa
     1321 aatggccgag tcgcgcggca gcaaatggtt caaggaccat tgcgtccatc cggtgcccta
     1381 tccctatccc ggcgcgcgcc ggctcgtcta tccgggcttc ctgcagctct ccggcttcat
     1441 ggcgatgaac ctcgatcgcc acgtcaatgc gcacatcgaa atgttcaacc atttgatgga
     1501 gggcgacggc gattctgccg agaagcaccg cgaattctac gatgaatatc tggcggtgat
     1561 gga
//
LOCUS       DASWOG010000011         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_220299,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000011 DASWOG010000000
VERSION     DASWOG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220299"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="nuoF"
                     /locus_tag="VGB93_00190"
     CDS             <1..952
                     /gene="nuoF"
                     /locus_tag="VGB93_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0144579.1"
                     /translation="CYIYVRGEYRLEFARVQAAIEEAYEANLVGQDNIHGWPFDIYVH
                     HGAGAYICGEESALLESLEGKKGMPRMKPPFPANMGLYGCPTTVNNVESIAAAPAILR
                     RGAAWFSSFGAENNKGTKLFCISGHVNTPCNIEEVMSIPFRELIERHCGGVRGGWDNL
                     LAVIPGGSSVPLVPAHEIIDAPMDFDTLRNLKSGLGTAAVIVIDKATDIVRAITRISY
                     FYKHESCGQCTPCREGTGWMWRVLTRMSEGRAQKREIDLLLEVSTDIEGHTICALGDA
                     AAWPVQGLIRHFRHEIEKKIDDYSANPHSEPLADLGVAAE"
     gene            956..3037
                     /gene="nuoG"
                     /locus_tag="VGB93_00195"
     CDS             956..3037
                     /gene="nuoG"
                     /locus_tag="VGB93_00195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY0144580.1"
                     /translation="MSEPAKLSKLIVDGIEVEVPPEYTLLQACEAAGAEIPRFCFHER
                     LSIAGNCRMCLVEAKGGPPKPVASCAMAVRDLRPGPKGEPPVVFTNSPMVKKARHGVM
                     EFLLINHPLDCPICDQGGECDLQDQAVAFGFSGSRYAENKRAVEDKYIGPLVRTSMNR
                     CIHCTRCVRFTAEVAGTGDMGAIGRGEDMEITTYLETAMGSELQGNIADLCPVGALLP
                     KPYSFKARAWELTKTESVDVMDALGSAIRVDTRGREVMRVLPRLNDAVNEEWISDKTR
                     QIVDGLKTRRLDQPYVRENGKLRPASWMEAFAAVAAKVKAAPAARIGALVGDLAAAEE
                     IFALKLLMQSLGVVSLDCRQDGSKLDPKFGRASYIFNPTIAGIDDADAIMIIGSNPRK
                     EAPVFNARIRKRWLKGNLLLGVIGENADLTYSYNHLGAGPESLARFIDHAPAQKERPL
                     LLVGQGALARPDGAAILAQAAKAAVSLGCIKPGWTGFGVLHTAAARVGALDLGFVPGE
                     GGLDALTMTQPGALDIIFNLGADEIDVAAGAFVIYQGTHGDRGAHRADVVLPGATYTE
                     KPGLYVNTEGRVQLAERANFPPGDAREDWAILRALSAVLGKTLPFDSLHALRQKLFAD
                     YPLFAKIDAIVPGDPADIANIADGGVTPGDAAFVSPVEDFFLTNPIARASAVMAECSA
                     LARGHLKQAAE"
BASE COUNT          567 a          950 c         1014 g          626 t
ORIGIN      
        1 ctgctatatt tatgtccgtg gcgaataccg gcttgaattc gcgcgcgtgc aggcggcgat
       61 cgaggaggcc tatgaggcca atctcgtcgg ccaggacaat atccacggct ggccctttga
      121 tatttacgtc caccatggcg ctggcgctta tatctgcggt gaagaaagcg cattgctcga
      181 atcgctcgaa ggcaaaaagg gaatgccgcg gatgaagccg ccgttcccgg ccaatatggg
      241 cctctacggt tgcccgacga cggtgaacaa tgtcgaatca atcgccgccg caccggcgat
      301 cctgcggcgt ggcgccgcct ggttctccag cttcggtgcc gagaacaaca agggcacgaa
      361 gcttttctgc atctccggtc acgtcaacac gccgtgcaat atcgaagagg tgatgtcgat
      421 cccgttccgc gaattgatcg aacgccattg cggcggcgtc cgcggcggct gggataatct
      481 gttggctgtg attccgggcg gttcttcggt tccgctcgtg ccggcgcacg agattatcga
      541 tgcgccgatg gatttcgata cgttgcgtaa tctgaaatcc ggccttggca ctgcggcggt
      601 catcgtcatc gacaaggcaa cggatattgt gcgcgccatc acccgcatca gctatttcta
      661 caagcatgag agctgcggcc agtgtacgcc ctgccgtgag ggcacgggtt ggatgtggcg
      721 cgtgctgacc cgcatgagcg aaggccgggc gcagaaacgc gagatcgatc tgttgctcga
      781 agtttcaacc gacatcgaag gccatacgat ctgcgcgctc ggcgacgcgg cagcatggcc
      841 ggtgcaaggc ctcatccggc atttccgcca tgagattgag aagaagatcg acgattattc
      901 ggccaatccg cattcggagc ctttggccga cctcggcgtg gcggcggagt aggtaatgag
      961 cgaaccagcc aaactcagca agctcatcgt cgacggcatc gaagtcgaag tgccgccgga
     1021 atatacgctg ctccaggcct gcgaagccgc cggcgcggaa atcccgcgct tctgcttcca
     1081 cgagcggctc tcgattgccg gcaattgccg catgtgtctc gtcgaggcca agggtgggcc
     1141 gccgaagccc gtcgcttcgt gcgcgatggc ggtgcgcgat ctgcgtcccg gcccgaaggg
     1201 cgagccacct gttgtcttca ccaattcgcc gatggtgaaa aaggcacgcc acggcgtcat
     1261 ggaatttctg ctcatcaacc atccgctcga ttgcccgatc tgcgatcagg gcggcgaatg
     1321 cgatttgcag gaccaggccg tcgccttcgg tttttccggc tcgcgctatg ccgagaacaa
     1381 gcgcgcggtc gaggacaaat atatcggccc gctggtgcgg acctcgatga accgttgcat
     1441 ccattgcacg cgctgcgttc gcttcacggc ggaggttgct ggcacgggag acatgggcgc
     1501 gatcggccgc ggcgaggaca tggaaatcac gacctatctc gaaaccgcga tgggttcgga
     1561 attgcagggg aatatcgccg atctctgccc cgtcggcgcg ctgttgccga agccgtattc
     1621 gttcaaggcg cgggcatggg agctgacgaa gaccgaatct gtcgatgtga tggatgcatt
     1681 gggctcggcc atccgcgttg atacgcgcgg gcgcgaagtg atgcgcgtcc tgccgcgtct
     1741 caacgatgcg gtgaacgaag aatggatttc cgacaagacg cgccagattg tcgatggcct
     1801 gaagacgcgc cggcttgatc agccctatgt ccgcgagaac ggcaagctgc gtccggcgag
     1861 ctggatggag gcctttgcag ccgttgcggc gaaggttaaa gcggcaccgg cagcgcgtat
     1921 cggcgcgttg gtcggcgatc tcgccgcggc ggaggaaatc ttcgcgctga agctactgat
     1981 gcaaagcctc ggcgttgtgt cgctcgattg ccgtcaggat ggctcgaagc tcgatccgaa
     2041 gtttggccgc gcgagctaca ttttcaatcc gacaattgcc gggatcgacg acgccgacgc
     2101 gatcatgatc atcggctcga acccgcgcaa ggaagcgccg gttttcaatg cgcgcatccg
     2161 caaacgctgg cttaagggca atctgcttct cggcgtgatc ggcgaaaacg ccgatcttac
     2221 ttattcctat aatcatctcg gcgcggggcc ggagagtctg gcgcgcttta tcgatcatgc
     2281 gccggcacag aaggagcggc cgttgctgct cgtcgggcag ggcgcgctgg cgcggccgga
     2341 tggcgcggcg atccttgcgc aagccgccaa agccgccgtt tcgctcggct gcatcaagcc
     2401 cggctggacc ggcttcggcg ttctgcatac cgctgcggcg cgcgtcggtg cgctcgatct
     2461 cgggttcgtg ccgggcgagg gcggccttga cgcgctgact atgacgcagc ccggcgcgct
     2521 ggacatcatc ttcaatctcg gcgccgacga gatcgacgtc gcggcgggtg ccttcgtcat
     2581 ctatcagggc acgcatggcg accgtggcgc gcaccgcgcc gacgtcgttc tgccgggcgc
     2641 gacctacacc gagaagccgg gcctttacgt caatacggaa ggccgcgtgc agcttgccga
     2701 gagggctaat tttccgcccg gtgatgcgcg cgaggattgg gccatcctgc gcgcgctttc
     2761 ggcggtgctt ggcaagacct taccattcga ctccctccac gcgctgcggc agaagctgtt
     2821 tgcggattat ccgctcttcg ccaagatcga cgcgatcgtt cccggcgatc cggcggacat
     2881 cgccaatatc gccgacggcg gcgtgacgcc gggcgatgcg gccttcgtct cgccggtcga
     2941 ggatttcttc ctgaccaatc caatcgcgcg cgcctcggct gtcatggccg aatgttcggc
     3001 gctggcgcgc ggccatctga aacaagcggc ggagtagcat cgatcatgga cgctctggcg
     3061 cttctattga cccttgtgaa gagcgtcgtg atgctcgtcg ttctgctgat tttcatcgcc
     3121 tacatccttt tagcggatcg caaggtctgg gcggcgg
//
LOCUS       DASWOG010000012         4326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_55372,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000012 DASWOG010000000
VERSION     DASWOG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55372"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1547)
                     /locus_tag="VGB93_00200"
     CDS             complement(258..1547)
                     /locus_tag="VGB93_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144581.1"
                     /translation="MTEFAVRGEGDLNKKSAGPSGLVQRVAHSPLRWVIAVVLLVSVV
                     TGFFDRISVAVLFTDHGFNAAMGTDFHPAALGMLMTAFLLAYALSSIFLSFGGDLFGP
                     RRTLGFASGVWGVLMFIMGACGSYGGMIFYRVLLGLSEGPQFSLISKTVQRWFPRHEQ
                     ARANALWMVGSPIGSAIGFPLTIWLVSNYGWRASFYVLGAFNFLLVMPLILGVVKDNP
                     PVANDAPAEVKRSAVRLADVRLLLADKKVWMLTGFGTGLLTCLWGLNSWLPTYLQRVR
                     HFDLHHMGIYSALPFILMFFAELIGGYIADRTGKYALLSSSGLFAAGVLMYIATQVEQ
                     PQLAAVIIAFSAAAWGFGIPTQYALALRVLPVGTTSTGIGIINGIGNLVGACAPAVIG
                     FIVAGTGSYKAGLLVIVVASVLGSLALLPMLRFKPAT"
     gene            complement(1622..2833)
                     /locus_tag="VGB93_00205"
     CDS             complement(1622..2833)
                     /locus_tag="VGB93_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0144582.1"
                     /translation="MTRQFKLVVVGAGPVGVVAALAAVQAGFDVTIFESALEVDHSPR
                     AATTHPSTLEMIHRLGLLDRFLAEGLVARDFQFWDRESHSLVATFDHDVLRDDTPFPF
                     AVQTEQHKLVHMGLDALRSLGCAIHFGTRVTSCVQNADRVTLDIETNGERATVEADWV
                     IAADGGRSTMRKNLGIEFEGYTWPERFIVLTVLDDMQKHMQCCYRNYLAGEGEWVSIF
                     KVAGDDGQGRWRVVSPTRPDETDEEALSDAAVAWRLERAYPLDRPYQLIHRNLYRVHQ
                     RVAAAFRAGRILLAGDAAHVNNPLGGLGLNFGIHDAMDAVVALAAVAMHGADEALLDN
                     YAQRRRALNIEYVQQQTIANKKRLEPKSPEARRADFDELRATAEDQVRARAFLLRSSL
                     ISSVREARIMA"
     gene            3047..3829
                     /locus_tag="VGB93_00210"
     CDS             3047..3829
                     /locus_tag="VGB93_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144583.1"
                     /translation="MDLQLQGKLALVTGGSAGIGRAVARLLTAEGCRVAIVGRDEARL
                     AALRAEPGMENTLTFRADLSEPEAAARVANDLFTQADVPDILVNSVGAAQGGLFWELD
                     DQVWNDAFNLKFMGTVRMLRAILPHMVARNSGNVVTIVGNNGRQPGPRFLPGSSANAA
                     LLALTKGLADEVAAKGVVINAVNPGPTRTGRWNGLMAKAAADSGRSVAEVEAGHVAAI
                     PRGVLSEPEEIARYVVFLCSPLATAALGTSFTIDGGATRALA"
     gene            3851..>4326
                     /locus_tag="VGB93_00215"
     CDS             3851..>4326
                     /locus_tag="VGB93_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0144584.1"
                     /translation="MSNPRIPYRLGSTQANLPLLHGKRLIVQFIVNIEAWLFDGKMPR
                     TIVTPPQGIEHIPDIPNFCWAEYGMRLGMKRLIDEFSSRGIPVGASINAGVIDIYPEC
                     AEAVRAAGWEFIAHGMYQKAIQGEADESGLICAVLDKIEAFTGKRPRGWLGPGLRQ"
BASE COUNT          939 a         1359 c         1250 g          778 t
ORIGIN      
        1 aacatcgccg caatggccga ccgctgaaga aataggctga tccccgtcat ttcgtccctc
       61 gtgtctaaaa agccgaatgc tggtgtcttc gactgccagt gccactcatc cacgcacgta
      121 cattgtcttg gtgtcagaca accctcacgc ggatcatgcg tgaactttgt caaaaaagcc
      181 cagggtgcgt tggacgcgcc ctgggccatt tcggattcaa gtcctcgaat tccggaaatc
      241 gcaggcgcgc gccgcgttca cgtcgcgggc ttgaaacgca acatcggcaa aagcgccagg
      301 gatccgagca cacttgcgac gacgatcaca agcaggccgg ctttgtagct gcccgtcccc
      361 gcaacgatga agccgatcac tgccggggcg caggcgccga caagattgcc aataccgttg
      421 atgatgccga tacccgtcga cgtcgtgcct accggaagta cacgcagcgc cagtgcatat
      481 tgcgtaggaa tgccgaagcc ccaggccgca gcgctgaatg caataatcac cgccgcgagc
      541 tgcggctgct ccacctgcgt cgcgatatac atcaggacac cagccgcaaa caggccggaa
      601 ctgctcaaga gcgcatattt gcctgtccgg tcggcgatat atccgccaat cagctctgcg
      661 aaaaacatga ggatgaacgg aagcgccgag taaatcccca tatgatgcag atcgaaatga
      721 cggacgcgct gcagataggt cggcagccag ctattcaggc cccagagaca ggtcagcaga
      781 cccgtgccaa agccggtcag catccagacc tttttgtcgg caagcaaaag gcgcacatcg
      841 gcgagacgga cggcgctgcg cttaacttcg gccggcgcat cgttggccac cggcgggtta
      901 tccttcacga cgccaaggat cagcggcatg acgagcaaaa agttgaatgc accaagcaca
      961 tagaacgagg cgcgccagcc gtaattcgag acgagccaga tagtcaaagg aaagccgatt
     1021 gcggacccga tcggacttcc aaccatccag agcgcattgg cccgcgcctg ctcgtgccgc
     1081 ggaaaccagc gttgtacagt cttcgaaatc aaagagaact gcggtccttc gctgaggccg
     1141 agcaacacgc gatagaagat catgccgccg tatgaaccgc aggcacccat gatgaacatc
     1201 agcacgcccc agacgcctga tgcaaagccg agcgtccggc gcggcccgaa aaggtcaccc
     1261 ccgaaactca aaaagatcga agaaagcgcg taggccagca aaaatgctgt catcagcatg
     1321 ccgagagccg cggggtgaaa gtccgtgccc atcgcggcgt tgaagccgtg gtctgtgaac
     1381 aggaccgcga cgctgatccg gtcaaaaaat ccggtgacga ccgaaacgag caggaccacc
     1441 gcgataaccc agcgtaacgg cgaatgagcc accctttgca ccagccccga cggccccgcg
     1501 ctttttttgt tcagatcgcc ctcacctcgc acggcgaact ccgtcataac cgaacccttt
     1561 cacgtcagca cggctaggaa tcggccgttt gtatgaatgt ctgacgatct acaatcaaga
     1621 atcaggccat tatccgtgct tcccggacac tgctgatcaa agatgatcgc aacagaaacg
     1681 cgcgggcgcg cacctggtcc tcggcggtcg cacgcagctc gtcgaaatcc gcccgccgcg
     1741 cctccggcga cttgggctca aggcgctttt tattggcgat tgtctgctgc tgaacatatt
     1801 cgatattcaa cgcgcggcgg cgttgtgcat aattatcgag cagcgcttca tccgcgccat
     1861 gcatggcgac ggcggcgagc gcaaccacag catccatcgc gtcatgaatg ccaaagttga
     1921 gccctaatcc gccgagcgga ttgttgacat gcgccgcgtc gcccgcgagc agaatgcgcc
     1981 cggctcgaaa cgccgctgcg acccgctgat gcacgcgata aagattacga tggatgagct
     2041 gatacggccg gtcaagcgga taggcgcgtt caagccgcca cgcgacagca gcatccgaca
     2101 acgcctcctc gtcggtttcg tccggccgcg ttggggacac gacgcgccag cgcccttgcc
     2161 cgtcgtcgcc cgcgaccttg aagatgctga cccactcgcc ttctcccgcg agataattgc
     2221 gatagcagca ctgcatgtgc ttctgcatat cgtcgagcac ggtgagaacg ataaaacgct
     2281 ccggccaggt ataaccctcg aactcaatgc cgagattttt ccgcatggtg ctgcggccgc
     2341 cgtcggcggc aatcacccaa tcggcttcga ccgtagcgcg ttcgccgtta gtctcaatat
     2401 ccaacgtgac acgatcggca ttttgcacgc acgaggtgac gcgcgtgcca aaatggattg
     2461 cgcagccgag actccttaag gcatcgagcc ccatatgcac gagcttgtgc tgctccgtct
     2521 gcaccgcgaa aggaaacggc gtatcatcgc gtaatacgtc gtgatcgaat gtcgcgacca
     2581 gcgaatggct ctcgcgatcc cagaattgga aatcacgcgc cacgagccct tcggcgagaa
     2641 agcgatccag caggcccagc cgatggatca tttcaagggt ggatggatgc gtcgttgccg
     2701 cgcgcgggct gtgatcgact tcgagcgcgc tttcaaaaat cgtgacgtcg aagcccgcct
     2761 gcacggcggc gagggccgca acgacgccga ctggcccggc gccgacgacg acaagtttga
     2821 attggcgagt caacgccttg ttccgcgtaa aataagcatt tccatctcag cctgactgag
     2881 gcggcgagga ctgtcgatgt ccattttggc ataacagata gagccgtcgc gtgctcgctg
     2941 aggcgcggaa accaatctat cgtccgcgtc gaaaagctcg tcacgctttt catttgcctc
     3001 cggaggccgg tcccagtgcc ggcctccttt tcaacaagga aagaatttgg atctgcagct
     3061 acaaggtaag cttgctctgg tcacgggcgg gagcgcgggg atcggccggg ccgtcgcgcg
     3121 tctcttgacc gccgaggggt gccgcgtcgc catcgttggc cgggacgaag cacgactcgc
     3181 ggccctgcgc gcagagcccg gcatggaaaa cacgcttaca tttcgcgccg atctgtctga
     3241 gcctgaagcg gccgcccgcg tcgcgaacga tcttttcacg caagccgacg ttcccgacat
     3301 cctcgtcaat agcgtcggcg ccgcgcaggg aggccttttc tgggaattag acgatcaggt
     3361 atggaacgat gccttcaacc tgaaattcat gggcaccgtg cgcatgttgc gcgcgattct
     3421 accgcatatg gttgcgcgca actcaggcaa tgtcgtcacg atcgtcggca acaatggccg
     3481 tcagccgggg ccacgttttc tgcccggctc ctcggccaat gcggcgctcc tcgcgctcac
     3541 caaaggcctc gccgacgagg tcgcggcgaa gggcgtcgtt atcaacgccg taaatccggg
     3601 ccccacacgc accggccgct ggaatggctt gatggccaaa gccgcggcag acagcggccg
     3661 cagcgttgcg gaggttgaag ccggtcatgt cgcggccatc ccgcgcggcg tcctgagcga
     3721 gccggaagaa attgcgcgct acgtcgtctt tctctgttcg ccccttgcta cagccgcact
     3781 cggcacgtcg ttcacgatcg acggcggcgc gacgcgcgcc ttggcgtgac gcaaacggtc
     3841 aaggagcctt atgtcaaacc cgcgtatccc ctaccgcctc ggctcgaccc aagcgaatct
     3901 gccgcttctg cacggcaagc ggttgatcgt ccaattcatc gtcaacatcg aagcctggct
     3961 cttcgatggg aaaatgccgc ggacgatcgt aacgccgccg caagggatcg aacatatccc
     4021 cgacattccg aatttctgct gggcggaata tggaatgcgc ctcggcatga agcgcctgat
     4081 cgacgaattt tccagccgtg ggattcccgt cggcgcgagc attaatgccg gcgtgatcga
     4141 catttacccc gagtgcgccg aggccgttcg cgcggctggg tgggaattca tcgcccacgg
     4201 catgtaccag aaggcgatcc agggtgaggc cgatgagtct gggctcattt gcgctgttct
     4261 cgacaagatc gaagctttca cgggtaaacg gccgcgtggc tggctcggcc ccggcctacg
     4321 ccaaag
//
LOCUS       DASWOG010000013         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_183663,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000013 DASWOG010000000
VERSION     DASWOG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183663"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VGB93_00220"
     CDS             complement(<1..608)
                     /locus_tag="VGB93_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0144585.1"
                     /translation="MNNLRLITGTSLALLLAGCAVGPNFVPPNPGLPATSFFAGATHK
                     PVVSTRVALQPVDPNWWALFHDRELTAIETRVAATNLDVRTATLRLVESRYQRGVTAS
                     SLFPTLDDNGSYKRYQISNSVLEGVAGNQLPPSALQPFDLWQQGFDASWELDIWGHVR
                     RQVEAADADIDAAADQRRGTLVSALAEVARDYMQLRGIQRQIR"
     gene            complement(720..2378)
                     /locus_tag="VGB93_00225"
     CDS             complement(720..2378)
                     /locus_tag="VGB93_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0144586.1"
                     /translation="MSAEQTLPFQGAGDAAPPTPAAQPKPVGAGARDPFDLGWAPKHN
                     PWAVAVVVTLAAFMEILDTTIVNVALPHIAGSLSSSNDEATWALTSYLVANGIVLTIS
                     GWLGSLLGRKRYFLICLAMFTVCSFLCGIATSLPQLILFRLLQGFFGGGLQPNQQSII
                     LDYFPPAKRGAAFGLTAIATIVGPVIGPTLGGIITDSASWRWIFFVNVPVGIAAVILV
                     YILVEDPPWVKRQPRPIDGIGLGLIALGLGCLEVMMDRGEDADWFGSSFIVIMAMLSV
                     TGLVGAAVWLSVAKKPIISLAIFKDKNFTGGCVMIGCVGAILYASSVIIPQYAQQVLG
                     YTATWAGMILSPGGLIIIILIPLVGRLMNVMQTRYIIAIGFFLLGLSFIYSSRLVLDI
                     TYLQLVMIRSSQTVGLAFLFVPISTITYSTLPRELNGDATSLFSMFRNVAGSIGIAGS
                     TAMIQQRSQVHQTYLAQWTTPFWQPFNNLVAKYQAALQALGNSASAAHQMALGHVYQD
                     FLKQVSVMAYSDVFKLCSIVAFALVPLCFTLSPIKGGRGGGGGH"
     gene            complement(2375..3529)
                     /locus_tag="VGB93_00230"
     CDS             complement(2375..3529)
                     /locus_tag="VGB93_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0144587.1"
                     /translation="MAKSRSDTDLPIAEDGSTQGAPAKAAPAPEPNAPPPAAKKKRRP
                     IVWIVLLALFGGLAFTGYEYWLANRNIEDTDDAFVDGNAVTISPQVSGNVVSLDITDN
                     QFVKKNQPLIHIDNRQYVIDVENAKANLATAEGQYAGFQYAAEVAKKNFPAQLDAAKA
                     QLQSAQAQVIRAQADYDRQKSLPTRATTQQEVDAATAALRQAEAQVAQAQAQVEQDSP
                     VHQRIGQAEASVSQFHGQIEQAKARLDQALLNLSWTIVQAPQDGWITKRNVNVGTYVT
                     PGQQIFSLVSPQVWITANFKESQLAHMRAGQPVDITVDAYPDLKLKGHVDSLQLGTGS
                     RFTAFPPENATGNFVKIVQRVPVKIVIDSGLDPKLPLSLGLSVEPRVKVQ"
     gene            3742..4356
                     /locus_tag="VGB93_00235"
     CDS             3742..4356
                     /locus_tag="VGB93_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0144588.1"
                     /translation="MAERVFLELGFNDTTMQIVASRAGASKETLYRHFGSKEGLFSEV
                     VRARAERVYGQVESEIGQRNDPRDVLKKLGYNLLGKLLSEDPLCLFRIVISEVQRTPE
                     LGRIFFEQGPNQLLKDLASYLEYATKQGMLKCQEPALAAKLFLGSVVANRHIIRLVVP
                     DYEDLTDEIIHHHVEAAVDMFLAAYGPRPDMRAPPTAALEIGHV"
BASE COUNT          930 a         1270 c         1419 g          865 t
ORIGIN      
        1 cggatctgcc gttggatgcc gcgcaattgc atatagtcgc gcgccacttc ggcgagcgcc
       61 gaaacgaggg tgccacgccg ctggtccgcc gcggcatcaa tgtcggcatc ggcggcttcg
      121 acctggcgcc gtacatgccc ccagatatcg agctcccacg aggcgtcgaa accctgttgc
      181 catagatcga agggctggag cgcactcggc ggtagctgat tacccgcgac gccctcaagg
      241 acgctgttac tgatctgata gcgcttgtaa gagccattat cgtcgagcgt gggaaacagg
      301 ctggaagccg tgacgccgcg ctgatagcgg ctttcgacaa gccgcagggt tgccgtccgc
      361 acatcgaggt tggtggcggc gacgcgggtc tcgattgcgg tcagttcgcg atcgtggaag
      421 agcgcccacc aattcggatc gacaggctga agcgcgacgc gcgtagagac aaccggcttg
      481 tgcgtcgcac ccgcaaaaaa cgaggtcgcc ggaaggccgg gatttggcgg gacgaaattc
      541 ggtcccacgg cacagccagc gagcaacaat gccagcgagg tgccggtgat caaccgcaaa
      601 ttattcacgc taagttaccc ccgcgtaatc ctatttaaac gaatagccgc cgcgccaaat
      661 ggcgtttcgc ggcgtttcgc cgactttatt ggtctgtgtg tctccgtcgc ctcagcgcgc
      721 taatggccac ctccgccgcc gcggccgccc ttgatcggcg acagcgtgaa gcagagcggc
      781 accagcgcga aggcgacgat ggagcagagc ttgaacacgt cagaataggc catcaccgaa
      841 acctgcttga ggaagtcctg atagacgtgg ccgagcgcca tctgatgtgc cgccgacgcg
      901 gaattcccga gcgcctggag cgcagcctga tatttggcga cgagattgtt gaacggctgc
      961 cagaacggcg tggtccattg cgccaaatag gtctgatgca cctgcgatcg ctgctggatc
     1021 atcgccgttg aacccgcgat gccgattgag ccagcaacat tgcggaacat cgaaaacagc
     1081 gacgtggcat cgccgttcaa ttcgcgcggc agcgtcgaat aggtgatcgt gctgatcggc
     1141 acgaagagaa atgccagtcc gacggtctgc gaactgcgga tcatgacaag ttgcagatag
     1201 gtgatgtcga gaaccagtct cgacgagtag atgaaggaaa ggccgagcag gaaaaagcca
     1261 atcgcaataa tgtagcgcgt ctgcattaca ttcatcagtc ggccgacaag cgggatgagg
     1321 atgatgatga tcaagccgcc gggcgaaagg atcatgccgg cccaggtcgc tgtatagccc
     1381 aggacctgtt gtgcatattg cggaatgatg acggacgagg cgtagagaat ggcgccgacg
     1441 cagccgatca tcacgcagcc gccggtgaag ttcttgtcct tgaagatggc aagactgatg
     1501 atcggcttct tcgcgaccga cagccacacc gccgcaccga cgagaccggt gacggagagc
     1561 atcgccatga tcacaatgaa gctcgaaccg aaccagtcgg cgtcctcgcc gcggtccatc
     1621 atcacttcga ggcagccgag accgagtgcg atgagaccga ggccgatacc gtcgatcggc
     1681 cgtggctggc gcttcaccca cggcggatcc tcgacaagga tataaacaag gatgaccgcg
     1741 gcgatgccga ccggcacgtt gacgaagaaa atccagcgcc aggacgcgga atcggtgatg
     1801 atgccgccaa gcgtcgggcc gatgactggg ccgacgatgg tcgcaatcgc tgtgaggccg
     1861 aaggcggcgc cgcgtttggc gggcgggaaa taatcgagaa tgatcgattg ctggttcggc
     1921 tgcaggccgc cgccgaagaa gccttgcagt agccggaaca ggatcagttg cggcaggctc
     1981 gtggcgatac cgcacagaaa cgagcagacg gtaaacatcg ctaggcagat gagaaagtag
     2041 cgcttgcggc cgaggaggga gccgagccag ccggaaattg tgagcacgat cccgttggca
     2101 acgagatacg acgtcaacgc ccatgtcgct tcgtcattgc tcgacgacag actgccggcg
     2161 atgtgcggca gcgcgacatt gacgatagtc gtgtctagaa tttccatgaa ggctgcaagc
     2221 gtcacgacga cggcaacggc ccagggattg tgtttcggcg cccagccgag atcgaacggg
     2281 tctcgcgcac cggcgccgac tggtttcggc tgcgcggctg gcgtcggagg tgcggcgtct
     2341 cccgccccct gaaatgggag cgtctgctcc gcgctcattg cacttttacg cgcggctcga
     2401 cagagaggcc gagcgagagc ggcagcttcg ggtcgaggcc ggagtcgatg acaatcttca
     2461 caggcacgcg ctggacgatc ttgacgaaat tgcccgttgc attttccggc ggaaaggcag
     2521 tgaagcgcga tcccgtcccg agttggagcg aatcgacatg acctttgagc ttcagatcgg
     2581 gataggcatc gactgtgatg tccacaggtt gacccgcgcg catatgggcg agctggcttt
     2641 ccttaaagtt cgccgtgatc cacacctgcg gcgagactaa agagaaaatc tgctggccgg
     2701 gcgtgacata ggtgccgaca ttgacgttgc gcttggtaat ccagccgtcc tgcggcgcct
     2761 gcacgatggt ccacgagaga ttgagcagcg cctgatcgag tctggctttc gcctgttcga
     2821 tttgaccgtg gaattgcgag acgcttgctt ccgcctgacc gatgcgttga tgcaccggcg
     2881 aatcctgttc gacctgcgcc tgcgcttgcg cgacctgggc ctcagcctgc cttaaggccg
     2941 ccgtcgccgc atcgacttct tgctgcgtcg tggcgcgggt gggcaacgat ttctgtcggt
     3001 cgtaatccgc ctgtgcccgg atgacttgcg cctgtgcgga ctgcaattgc gctttggcgg
     3061 catcgagctg cgccgggaag ttcttcttcg cgacctcggc agcatattgg aagccggcat
     3121 attgcccttc cgccgtggcg agattcgctt tcgcgttttc gacgtcgatc acatattgcc
     3181 ggttgtcgat atggatcagc ggctgattct ttttgacgaa ttgattgtcg gtgatgtcga
     3241 gcgagacgac attgcccgaa acctgcggcg aaatggtcac cgcgtttccg tcaacgaaag
     3301 cgtcgtccgt atcttcgata ttgcgattgg cgagccaata ttcgtagccg gtaaaggcga
     3361 gtccgccgaa aagcgccaac agcacgatcc agacaatcgg gcggcgcttc tttttcgctg
     3421 ccggtggggg agcattcggt tcgggtgcag gcgcggcttt cgccggcgcg ccttgcgtcg
     3481 agccatcttc agcgatgggg agatcggtgt cggaacgcga tttggccatg agtgtgcatc
     3541 gcaacagttt gcggcggggc aaaccaactc atttgggttt gccgccggcg catccggtga
     3601 aatttgtcaa aatcggactt aaccgaactg atgagttcgt atatggtggc ggggaaatga
     3661 tgtcaaccga aggaccccag agaaatgttg aggacgaggc gaatccctcg cggcgagaag
     3721 cggcgtgacg aaattgcagc agtggccgag cgtgtttttc tggagctcgg ctttaatgac
     3781 accacgatgc agatcgtcgc ctcgcgggcc ggcgcctcga aggagacgct ttatcgacat
     3841 ttcggcagca aagagggtct tttctcggaa gttgtacgcg cccgcgcgga acgcgtctac
     3901 ggccaggtcg aaagcgagat cggtcagcgc aacgatccgc gggatgtttt gaagaagctc
     3961 ggctataatc ttctcggcaa gctcctgagc gaggatccgt tgtgcctgtt caggatcgtc
     4021 atttcggaag tgcagcgcac gccggagctt ggccgcatct tctttgagca aggccccaat
     4081 caattgttga aggacctcgc gagctatctc gaatatgcga cgaaacaagg catgctgaaa
     4141 tgccaggagc cggctctcgc cgccaagctg tttcttggct cggttgtcgc caatcggcat
     4201 atcatccggc ttgtggtccc cgattacgag gacctgacgg atgagatcat ccatcatcac
     4261 gttgaggcgg ccgtcgatat gttcctcgcg gcctacggcc cgcgtcctga tatgcgcgcg
     4321 ccaccgacag cggctctgga aatcggccat gtctgaggag atgccgcgcg aggcattgac
     4381 acaactcctg cgctggtatg ccgacatggg cgtcgacctc gcggtcgacg acatgccgca
     4441 tgatcgtttc gcggaatcgc aaagcccgga aaaggctgcg ccgc
//
LOCUS       DASWOG010000014         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_110377,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000014 DASWOG010000000
VERSION     DASWOG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110377"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGB93_00240"
     CDS             <1..397
                     /locus_tag="VGB93_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEY0144589.1"
                     /translation="GIEQYYSPEEVYEHPANPFVFRFLGNYNLFHGRQASHALGQGDA
                     GGENVTFVRPHDIEISRESDASAIAAKVTHIGFAGAAVNVELIRLDDQRSIDAELTTQ
                     TYRDLALKVEDQVFVRLRNAHSFADDYSI"
     gene            606..797
                     /gene="secE"
                     /locus_tag="VGB93_00245"
     CDS             606..797
                     /gene="secE"
                     /locus_tag="VGB93_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY0144590.1"
                     /translation="MADPLQFIQEVRQEATKVTWPTRRETLITTGLVITMVILASLFF
                     LAVDEILQLVVRFVLNVGR"
     gene            822..1352
                     /gene="nusG"
                     /locus_tag="VGB93_00250"
     CDS             822..1352
                     /gene="nusG"
                     /locus_tag="VGB93_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0144591.1"
                     /translation="MNMRWYIVHAYSNFEKKVAESIREQAAQRNLSEKFEEILVPTEH
                     VVEVRKGRKVNSERKFFPGYVLVKCDLTDDIYHLIKNTPKVTGFLGADKKPMPISDAE
                     ADRIKGQVADGVDRPKSTISYEIGETVRVADGPFASFNGTVEEVDEARSRVKVAVSIF
                     GRATPVELEYSQVEKV"
     gene            1496..1924
                     /gene="rplK"
                     /locus_tag="VGB93_00255"
     CDS             1496..1924
                     /gene="rplK"
                     /locus_tag="VGB93_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0144592.1"
                     /translation="MAKKIAGYIKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTAQMEKGTPIPVVITAYQDRSFTFEMKLPPVTFFLKKAAGITSGSKTTGRGFVGRVT
                     KAQINEIAEKKLPDLNCATVEAAAKMIEGSARSIGLEVVG"
     gene            1929..2624
                     /gene="rplA"
                     /locus_tag="VGB93_00260"
     CDS             1929..2624
                     /gene="rplA"
                     /locus_tag="VGB93_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0144593.1"
                     /translation="MTHIAKRVVKSREGIERTKLYPISEAVKLVKERASAKFDETVEI
                     AMNLGVDPKHADQMVRGVVNLPNGTGRTLRVAVFARGAKADEAKAAGADIVGAEELVA
                     TVQGGTIDFDRCIATPDMMPLVGRLGKVLGPRGLMPNPKVGTVTMDVTAAVKASKGGA
                     VEFRVEKAGIIQGSVGKASFDETKLVENIAAFVDAVAKAKPQGAKGTYIQRVAISSTM
                     GPGVKVDPATVHV"
BASE COUNT          540 a          782 c          862 g          531 t
ORIGIN      
        1 tggcatcgag caatattatt cgcccgagga agtctacgag catccggcga acccgttcgt
       61 cttccgcttc ctcggcaatt acaatctgtt ccacggcaga caggccagcc acgccttggg
      121 gcagggggac gccgggggcg agaacgtcac tttcgtgcgg ccgcacgaca tcgaaatctc
      181 ccgcgagagt gacgctagcg ccatcgcggc caaggtgacc catatcggct ttgcaggggc
      241 cgccgtgaat gtcgagctca tcaggctcga tgatcaacgg tcgatcgatg cggagctgac
      301 aacgcagacc tatcgcgatc tggcgctgaa ggtcgaagac caggtcttcg tccgcctgcg
      361 caatgctcat tccttcgccg acgattactc gatctgaggc ccgggcggag gtcagttgag
      421 acttgctgag aattcgcttg gcgcggccaa tcgcctgcac tatattgtcg aggtgtggcg
      481 cgtggccggt tggctccgcg ccctgatttt tttagccgag gccacgcggg cgatatcggc
      541 ggccgcagcc gccggagttt gccgcggcca ggcgaaaaat tcctgcaaat tgggtgttag
      601 cgactatggc cgatccgctg caattcattc aggaggtccg tcaggaggct accaaggtca
      661 cttggccgac ccgccgcgag acgttgatca cgaccggtct ggtcatcacc atggtcattc
      721 tcgcgagcct gttttttctc gccgtcgatg aaattttaca attggttgtc cgcttcgtcc
      781 tgaacgtcgg acgttgattc gaggggtttt tcggtttcgt catgaacatg cgctggtaca
      841 tcgtccacgc ctattccaat ttcgagaaga aggtcgccga atcgatccgc gagcaggcgg
      901 cgcagcgcaa tctgtccgag aagttcgagg aaatccttgt gccgaccgaa catgttgtcg
      961 aggtgcgcaa gggccgcaag gtcaattccg agcggaagtt cttccccggc tatgtgctcg
     1021 tcaaatgtga cctgacggat gacatctatc acctgatcaa gaacacgccg aaggtgacgg
     1081 gcttccttgg ggcggacaag aagccgatgc cgatctcaga cgccgaggcg gaccggatca
     1141 agggccaggt ggccgatggt gtcgatcggc cgaagtcgac gatctcctac gagatcggcg
     1201 agacggtgcg ggttgccgac ggcccgttcg cgtccttcaa cggcacggtc gaggaagtcg
     1261 acgaggcccg ctcgcgtgtc aaggtggcgg tctcgatctt cggccgcgcc acgccggtcg
     1321 aactcgaata ttcgcaggtc gagaaggttt aagaattttc ggtgcttgca ctgaaatggg
     1381 cgatgcccta aaggctcgcc ttccgctgtg ggagaaaggt ccggtttgcc gccgggtcgc
     1441 cttttgtacc acgatccgca actccgatct gaggatcggc ttgtagggga ttgaaatggc
     1501 aaagaaaatt gccggttata ttaaattgca ggtgccggcc ggcgccgcca atccgtcgcc
     1561 gcctatcggc ccggcgctcg gtcagcgcgg cctcaacatc atggaattct gcaaggcctt
     1621 caacgcgaag accgcgcaga tggaaaaggg gacgccgatc cccgtcgtga tcaccgctta
     1681 tcaggaccgc tccttcacct tcgagatgaa gctgccgccg gtgacctttt tcctgaaaaa
     1741 ggccgcaggc atcacctccg gttcgaagac cacgggccgt ggctttgtcg gccgcgtgac
     1801 caaggcgcag atcaacgaga tcgccgagaa gaagctgccg gatctgaatt gcgcgacggt
     1861 cgaggccgcc gccaagatga tcgagggctc cgcccgctcg atcggcctcg aagtggtggg
     1921 gtgaagctat gacccatatt gcaaagcgcg ttgttaaatc ccgcgaaggc atcgagcgca
     1981 ccaagcttta tccgatcagc gaggccgtaa agctcgtcaa ggaacgggcg agcgccaagt
     2041 ttgacgagac ggtcgagatc gccatgaacc tcggcgtcga tcccaagcat gcggatcaga
     2101 tggtccgtgg cgtggtcaat ctgccgaacg gcacgggccg cacgctgcgc gtcgccgtct
     2161 tcgctcgcgg cgccaaggct gatgaggcta aggcggccgg cgcggacatc gttggcgccg
     2221 aggaactggt ggcaaccgtc cagggtggca cgatcgattt cgatcgctgc atcgcgaccc
     2281 cggatatgat gcccctcgtc ggccgtctcg gtaaggtgct gggcccgcgc ggcctgatgc
     2341 cgaacccgaa ggtcggcacg gtgaccatgg acgtcacggc tgcggtcaag gcatcgaagg
     2401 gcggtgcggt cgaatttcgt gtggaaaagg ccggcatcat tcagggcagc gtcggcaagg
     2461 cgtcgttcga cgagacgaag ctcgtggaga atatcgcggc cttcgtcgat gcggtcgcca
     2521 aggccaagcc gcagggcgcg aaaggcacct atattcaacg ggttgctatc tcgtccacga
     2581 tgggcccggg ggttaaggtc gatccagcca ccgtgcatgt gtaagagcgc cggggactgg
     2641 ccatcgggct gcttgacaag ctgaaaaaaa gctgaatccg ccccttggca tatcggggtc
     2701 ttgcgtttaa ataga
//
LOCUS       DASWOG010000015         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_128730,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000015 DASWOG010000000
VERSION     DASWOG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128730"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGB93_00265"
     CDS             <1..471
                     /locus_tag="VGB93_00265"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0144594.1"
                     /translation="REGFIDRQDWRRDAVAMTATHDLPTVAGWWRGIDIKFRAEQGLL
                     ADESEEEAARSAARKDLWRAFTAANIASGTPPPLDTPGAVVDNAIAFVAGSSSPLVLL
                     PLEDLLGLEAQPNVPGTIDEHPNWRRRYPVAAAEIFDAPEVAARAKVLSERDEA"
     gene            468..3071
                     /gene="treY"
                     /locus_tag="VGB93_00270"
     CDS             468..3071
                     /gene="treY"
                     /locus_tag="VGB93_00270"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY0144595.1"
                     /translation="MTVPRATMRIQFHRDFTFNDAIEIIPYMDRLNVSHLYASPILKA
                     RAGSMHGYDVVDPTQINPELGGESAFRALVGVLRAKGLGLIVDIVPNHMAVGGDDNAW
                     WLDVLQHGQQSSYAKFFDIDWNAPGLSGKILVPFLGGPYADIVASGDLKLVHNDNAGR
                     YELSYFQHRFPIRPRDHDEIAKYSAAHFDGRTEPGRQHLHQLLEKQNYHLAWWKIAND
                     TINWRRFFDINELAALRPEDDEVFEATHQKIFQLYANGLIDGVRIDHIDGLSDPGVYC
                     RKLRSRLDQLTSKRPGLRTRPYIVVEKILGAGESLPIEWRTDGTTGYDFMDQVARVLH
                     DWHGAMPLQQLWHGISHRPEMFLDEEEVARRAILARSFAAQLEGAVDSLCAISGSDLI
                     SHDVSRPAIRRVLVEILVHMRVYRTYARVGEALSADNVHLDAALAGAARSCLPSDRDM
                     LRRVAAWLDGGSNTAGDVGRRAEAIRKFQQLTAPLAAKAVEDTAFYRYGALLSRLDVG
                     FDAASFGGDIAAFHAEMETRLKTFPHAMLATATHDHKRGEDVRARLAVLSEMPAVWEA
                     SLCSWLDQAAPFFSTIEGKTAPSLADATLLFQMIVGAWPLDLSHEDAQGCAAFAERLV
                     AWQMKALREAKLETDWSVPNASYESAAKDFLTNLFREGWPERIYAFVDRIAPAGALNG
                     LTQTLLKLTVPGVPDLYQGTEFWDFSLVDPDNRRPVDFSARARSLNKDVTWSALVQNW
                     RDGRIKQTMIARTLEARRRWPELFGAGAYLPIETHGPAAVHLIAFARIHEGRVALIVC
                     PRLSSGRLDDGIVPVPRRWEGTELYLPQGFIFPPLRDAFGEAIVPGAAQVLEVASLLR
                     HTPVALLFSAE"
BASE COUNT          618 a          871 c         1000 g          689 t
ORIGIN      
        1 agggagggct ttatcgatcg gcaagattgg cgacgtgacg ccgttgcgat gacggcgacg
       61 cacgatcttc cgacagtcgc gggttggtgg cgtggcatcg acatcaagtt ccgcgccgag
      121 caggggcttc tcgcagacga aagcgaagag gaagccgcgc gttcggcggc gcggaaggat
      181 ttgtggcgtg ccttcaccgc cgcgaatatc gcgtcgggaa cgccgccgcc gctggacacg
      241 ccgggtgccg tcgttgacaa cgcgattgct tttgtcgccg ggtcttcctc gccactggtt
      301 ctgttgcctt tggaggatct gcttgggctc gaagcgcagc caaacgtgcc gggtacaata
      361 gacgagcatc caaactggcg ccggcgctat cccgtcgcgg cggccgaaat tttcgacgcg
      421 ccggaggttg ccgcgcgggc gaaagtgctc agcgaaaggg atgaagcgtg acagtccctc
      481 gcgcgacgat gcggatacag tttcatcgcg actttacctt taacgacgcg atcgaaatca
      541 tcccgtatat ggatcggctg aatgtgagtc atctgtatgc atcgccaatc ctgaaagcac
      601 gcgccggctc gatgcatggc tacgatgttg tcgatccgac gcagatcaat ccggaattgg
      661 gcggtgagtc ggccttccgg gcgttggtgg gcgtccttcg agcaaagggc ctcggactaa
      721 ttgtcgatat cgtgccgaac catatggccg tgggcggcga tgataatgcc tggtggctcg
      781 acgtgctcca gcacgggcag cagagctcct atgcaaagtt tttcgacatt gactggaatg
      841 cgcccggcct gtctgggaaa atattggtgc cgttcctcgg cggcccttac gccgacatcg
      901 tggccagcgg cgatctcaaa cttgttcata acgacaatgc gggtcgatat gaactttcgt
      961 acttccagca tagatttccg atccggccgc gagaccatga cgagattgcc aaatattcgg
     1021 ctgcgcattt tgacggtcgt acagagccgg ggcgacagca cctgcaccaa ttgcttgaaa
     1081 agcaaaatta tcacctcgcc tggtggaaga tcgccaacga cacgatcaac tggcgccgct
     1141 tcttcgatat caacgagctc gcggcgctgc gtccggaaga cgatgaagtc ttcgaggcga
     1201 cgcatcaaaa aatctttcaa ctctatgcga acggtttgat tgacggcgtg cggatcgatc
     1261 acatcgatgg tctttccgat cctggcgtgt attgccgcaa attgcggtcg cggctcgatc
     1321 agctaacgtc gaagcgtccc gggcttcgca cgcgccctta catcgtggtc gaaaaaatcc
     1381 ttggcgccgg tgagtcgctg ccgattgagt ggcgcaccga tggaacgacc ggctatgatt
     1441 ttatggatca ggtcgcgcgc gtccttcacg attggcacgg ggcaatgccg cttcaacagc
     1501 tttggcatgg gataagccac cggcccgaga tgtttttaga cgaagaggag gttgcgcggc
     1561 gcgctattct cgcacgcagc tttgcggcac agcttgaggg cgcagtggat tcgctgtgcg
     1621 cgatttccgg ttcggacctg atctctcacg atgtttcacg gccggcgatt cgccgtgtgc
     1681 tggtggagat tctggtacat atgcgtgtct ataggaccta tgcgcgcgtg ggggaggcat
     1741 tgagcgcaga caatgtccat ctggatgcgg ctttggcggg cgcggcgcgg tcctgcctcc
     1801 cgtcggacag ggacatgctg agacgtgttg cggcgtggct cgacggtggc tcaaatacgg
     1861 ccggcgacgt aggacgccgg gctgaggcga tacgaaaatt tcaacaactg accgcgccgc
     1921 tggcggcaaa agcggtcgaa gatacggcgt tttaccgata cggcgcgctg ctctcgcgtc
     1981 tcgacgttgg tttcgacgca gcttcgtttg gcggggatat cgcggcgttc catgccgaga
     2041 tggaaacacg cctcaagact tttccgcatg ccatgttggc gacggccacc cacgatcata
     2101 aacgcggcga ggacgtgcgg gcccggttgg cggtgctcag cgaaatgccg gccgtgtggg
     2161 aggcgagtct gtgtagctgg ctcgaccaag ccgcgccatt tttctcgacg atcgaaggga
     2221 aaacggcgcc ttcactcgct gacgcgacgc ttttgtttca gatgattgtc ggcgcatggc
     2281 ctttggattt gagccatgaa gacgcgcaag gctgtgccgc tttcgccgag cggcttgtgg
     2341 cctggcagat gaaagccttg cgtgaggcta aactcgaaac cgattggtcg gtgccaaatg
     2401 catcctacga aagtgcggcc aaggattttc tcacgaacct ctttcgggaa ggctggccag
     2461 aaagaattta tgctttcgtc gatcggattg cgcccgctgg ggcgttgaac ggtcttacgc
     2521 agacattatt gaaactcacg gtgcccggcg tcccagattt atatcaaggc acggagttct
     2581 gggattttag cctcgtcgat ccggataacc ggcgtcccgt tgatttcagc gcacgagcgc
     2641 ggagcctgaa taaggacgtc acatggtcag cgctcgtgca aaattggcgc gacggccgga
     2701 tcaagcagac gatgatcgcg cgtactctcg aagcccggcg acggtggccg gagctttttg
     2761 gagcgggcgc gtatctgcca attgagacac acgggccagc agcagtgcat ctcatcgcct
     2821 ttgcgcgaat tcatgagggg cgcgtcgcgc ttatcgtttg tccccggctc tcaagcggac
     2881 gcttggacga tggtatcgtt ccggtaccac ggcgctggga ggggacagaa ctttatctgc
     2941 cgcaaggatt tatatttccg ccattgcgcg atgcgttcgg cgaagcgatc gttcctggtg
     3001 ccgcacaagt cttggaagtt gcgtctctgc tgagacatac cccggtggcc ctgctttttt
     3061 ccgcggaatg agccgcgaca ggagcgcgcg atttgctgcc atgcgaaaac aacgatttcc
     3121 ctcgcgatct ttgcgggact tcgattatgt cgcgccctca tgcgagagat ggggaagg
//
LOCUS       DASWOG010000016         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_92091,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000016 DASWOG010000000
VERSION     DASWOG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92091"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1115)
                     /locus_tag="VGB93_00275"
     CDS             complement(162..1115)
                     /locus_tag="VGB93_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0144596.1"
                     /translation="MAARPYWRGYLKLSLVSCPIVLYPATSTAERVAFRQINKNTGNR
                     LRQQLIDEVTREPVETEDKGRGYEYAKNSYLQVDDAELDELQIESSKTIDIDTFVPRA
                     QIDERYFNSSYYIVPDGKVGQEAFSVIREAMRDKKMAALARVVLAKREHVMMLQPWDK
                     GMMGTTLRYPYEVRKADELFSEIEDFKVAPDMMKLADHIVESKTANFDPEQFHDRYED
                     AVVELIRSKRAGLPTAPQRVSENAPNVISLMDALKKSLAEGTRTPAAAKPAPAKGGAK
                     KPAKRAAGQTEMIMPIEGKKPKVAAPTAKPGPRSTTRKKTG"
     gene            1393..1602
                     /locus_tag="VGB93_00280"
     CDS             1393..1602
                     /locus_tag="VGB93_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144597.1"
                     /translation="MVGKRVQFDDETWAAINLLRQERRRSFQQLANEAFADLLAKYHR
                     PVDLKSQLRESLGQNPASRGKQAKG"
     gene            complement(1599..1790)
                     /locus_tag="VGB93_00285"
     CDS             complement(1599..1790)
                     /locus_tag="VGB93_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144598.1"
                     /translation="MPELKDVNDGKEWSETDIEDLRRALEHDASIEDAGRFLHRATAL
                     DEIAHKAIELGLKTAAGPD"
BASE COUNT          341 a          553 c          494 g          441 t
ORIGIN      
        1 ccgcgggcgt ggtccgtgcc actgcctcaa tcgaaactgc gttgccaatg cgaagcgtaa
       61 agatgctttt catctccacc ggatgcggcg cgcttcccga gccgagttca gcgatttcct
      121 tggaatgatt tttacccata agattcctgc gcgctcagag ctcagcccgt cttcttccgg
      181 gtcgttgatc ggggccccgg cttagcagtg ggtgccgcga ccttcggctt cttgccctcg
      241 atcggcataa tcatctcggt ctgacctgcg gcccgctttg ccggcttctt ggcgcccccc
      301 ttcgccggtg ctggtttcgc cgcggccggc gtccgcgttc cctcggccaa gctcttcttc
      361 aacgcgtcca tgaggctgat gacgttaggc gcattttccg aaacgcgctg cggcgcggtc
      421 ggcaggccgg cacgcttcga ccggatcaat tcgaccacgg catcttcata ccgatcgtgg
      481 aattgctccg gatcgaaatt ggcggttttg ctctcgacga tgtggtccgc gagtttcatc
      541 atgtcgggcg caaccttgaa atcctcgatc tcgctgaaaa gctcgtcggc cttccgaacc
      601 tcgtagggat agcgaagcgt tgtgcccatc atgcccttgt cccacggctg gagcatcatg
      661 acgtgctcgc gcttggcgag aaccacgcgc gcgagggccg ccattttctt gtccctcatc
      721 gcctcgcgaa taacgctgaa ggcttcctgt ccgaccttgc cgtctggcac gatgtaatag
      781 ctgctgttga aatagcgctc gtcgatctgc gcccgcggca cgaaggtatc gatgtcgatc
      841 gtcttactgc tctcgatctg aagttcatcg agttcggcgt catcgacttg aagatagctg
      901 ttcttcgcgt actcgtagcc cctgcccttg tcttccgtct cgaccggctc gcgcgtcact
      961 tcgtcgatga gctgctggcg caaacgattg cccgtatttt tattgatctg gcgaaaggcg
     1021 acgcgttcag ccgttgatgt cgcaggataa agaacgatcg gacacgagac gagggagagc
     1081 ttgaggtatc ctctccaata tgggcgagcg gccataaaaa cctcctacgc aaacgcaatg
     1141 aacaaatctg agccgtcggg cgcacgagtc aacccgatta aaattggaag atattttgcg
     1201 tcagcttaac ttagagcgga gctttaagtt cgcttcggcg ttgagttttt ccaaacgagg
     1261 gatcgcaatg cttaaattca tcaaagccgt tgtccgccac agcttgggcg taacttgttc
     1321 ttggctgggc aacaacaccg cgcactttta tggcgtgctc gtttcctccc cgaacgaccg
     1381 cacctagccg cgatggtcgg caagcgtgtc caattcgacg acgagacctg ggcggccatt
     1441 aatctgttgc gtcaagaacg gcgccgcagc tttcagcagc tcgccaacga ggcgtttgcc
     1501 gatttgctgg ctaaatatca tcggcccgtc gatctgaaat cgcagctacg ggaaagtctg
     1561 ggccagaacc cggcgtctcg gggcaagcag gccaaaggct aatcgggtcc ggccgccgtc
     1621 ttcagcccca attcgatcgc cttatgcgca atctcgtcga gcgccgttgc cctgtgtaga
     1681 aagcggccgg cgtcctcaat cgatgcatcg tgttcaagcg cacgccgcaa atcttcgata
     1741 tcagtttccg accattcttt gccatcgttc acgtctttta attcgggcat tcgtcgggct
     1801 caatctcgct gcaaatatct tatcgctcc
//
LOCUS       DASWOG010000017         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_348678,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000017 DASWOG010000000
VERSION     DASWOG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348678"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VGB93_00290"
     CDS             complement(<1..286)
                     /locus_tag="VGB93_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEY0144599.1"
                     /translation="MAETNNNENGNVPLSQLDRLVARARAVLFWEQLWRALVPPLFLA
                     GFFVAVSFAGLWLEIGALWRELGVGLFGIAFIAVLVPFFGVRLPARKAALA"
     gene            522..794
                     /locus_tag="VGB93_00295"
     CDS             522..794
                     /locus_tag="VGB93_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0144600.1"
                     /translation="MANADKLTTRISTKGQIILPSTIRQRRELTAGTRLVVEETAEGV
                     LLKPAPAFPETQPKDVFGSLPYKGKPKTLGEMDLGVLAEAKRHAGN"
     gene            781..1170
                     /locus_tag="VGB93_00300"
     CDS             781..1170
                     /locus_tag="VGB93_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007699842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0144601.1"
                     /translation="MLAIDANLIVRYMTDDHPKQSPRARALIDGHAVFVTITVILEVE
                     WVLRSAYGYRSVEVVRALRAFAGLPTVTMEDSSTVAAALDLSEKGMDFADALHLEKSA
                     HCQGFITFDRKFIKTAKTAGCDNVREA"
     gene            complement(1218..2486)
                     /gene="lysA"
                     /locus_tag="VGB93_00305"
     CDS             complement(1218..2486)
                     /gene="lysA"
                     /locus_tag="VGB93_00305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY0144602.1"
                     /translation="MHHFALKDGVLHAEGVNIDRLAAEVATPFYCYSTATLTRHYEVF
                     KRSFGELPVLVCYAVKANSNQAVLKTLGQLGAGMDVVSEGELRRARAAGIPAAKITFS
                     GVGKTAVEISTALDEDIMCFNVESEPELAAISQIASARGRTARIALRVNPDVDARTHA
                     KISTGKAENKFGIPLARAREVYAHAAALPGIEVTGIDIHIGSQITDLEPFDNAFALVA
                     AFLNVLAADGHKIEHIDLGGGLGIPYRPDDKGDAFHPDLYAKIVRRHFGGLGKQLIFE
                     PGRLIAGNAGILVTRVLYVKKAEAKTFVIVDAGMNDLIRPTLYDAHHEIIPVQPRPGA
                     PEILADVVGPVCETGDFLALNRTMPGPQPGDLLAVLSAGAYGAVQAGTYNSRLLVTEV
                     LVNGADYALVRPRETYEQLIGLDKLPPWLR"
     gene            complement(2494..2751)
                     /locus_tag="VGB93_00310"
     CDS             complement(2494..2751)
                     /locus_tag="VGB93_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY0144603.1"
                     /translation="MNPSSCRRCGVFGLVFLALALAGCGRRGDLEPPPNTPASQVVPM
                     QTKNIDPMAGDPAQPGRKPTPANSGTQAVSGNTFLLDPLVK"
     gene            complement(2825..>3493)
                     /locus_tag="VGB93_00315"
     CDS             complement(2825..>3493)
                     /locus_tag="VGB93_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY0144604.1"
                     /translation="AAALCAVHLSRFAEEIVLWTTPQFGFLRLSDRFSTGSSIMPQKR
                     NPDAAELVRGKSGRVIGALSGLLVVLKGLPLAYSKDMQEDKEGTFDALQTLSLCLAAM
                     TGMVGDFAPDLARMKAAAGAGYATATDLADYLVRELGLPFREAHHVTGHLVALAAERG
                     IGLEALSLADMQKVEPRLGEDVFAVLGVENSVASRTSYGGTAPANVAKQAQAWLDRLK
                     KPGN"
BASE COUNT          725 a         1079 c         1066 g          623 t
ORIGIN      
        1 gggcgagcgc cgccttgcgc gccggaaggc gcacgccgaa gaacggcaca aggacggcga
       61 tgaaggcaat gccaaagagg ccaacgccta gttcacgcca aagcgcaccg atctctagcc
      121 acagaccggc gaaagagacc gcaacgaaaa agccggccag gaacagcggc ggcacaagag
      181 cgcgccaaag ctgctcccag aaaaggacag cgcgagcgcg ggcgaccagc cggtcgagct
      241 ggctcaatgg cacattccca ttctcgttat tgttggtctc ggccaatgac ggctccggat
      301 gtgcccccgc gcaggggtag atcgcaattg ggtccgcgaa agggcgatta tccggtctta
      361 tggcctaaat gccggaagac ttccacgcca agcaagctgt cggccagata gtgccggcct
      421 gctctgttcg ccataccggc cgcccccctc ttaagaggcc ttgaatgcct gataccttaa
      481 cgctatctta cgttcgtctt actttaatgt ggatcagccg gatggctaac gccgataagc
      541 tcacgactag gatttctaca aaggggcaga tcatcctgcc gagcaccatt cggcagcgcc
      601 gggaattgac tgcgggaacg cggctcgtcg tcgaggagac ggcggaaggc gtgctgctga
      661 agccagcgcc ggccttccct gaaacgcagc ccaaggacgt gttcggctca ctcccctaca
      721 aaggcaagcc gaagacactg ggggaaatgg atttgggcgt gctcgctgag gccaagcggc
      781 atgctggcaa ttgatgccaa tttgatcgtc cgttacatga cagacgatca tccgaaacag
      841 tcgccacgcg ctcgcgccct gatcgacgga cacgcggttt tcgtcactat aaccgtgata
      901 cttgaagtcg aatgggtcct gcgtagtgcc tacggatatc gctcggtgga agtggtccgc
      961 gcgctaagag cattcgcggg ccttcccacc gtgacgatgg aagacagttc gaccgtggcg
     1021 gccgcgctcg atctttccga gaagggaatg gatttcgctg atgcgttgca tttggaaaag
     1081 tcagcgcatt gccaaggatt catcaccttc gatcgcaagt tcatcaagac ggccaagacg
     1141 gcaggttgtg ataatgttcg tgaagcctga cacggacaag acatcaatcg cacgacactc
     1201 ccgctctgga gtgcgaatta tcggagccaa ggcggcaatt tgtcgaggcc gatcaattgc
     1261 tcataggtct cgcgtgggcg caccagggcg taatcggcgc cgttgacgag cacttccgtg
     1321 accagaagcc gcgaattata ggtgccggcc tgaaccgcgc cgtaggcgcc tgccgacaac
     1381 acggcgagaa gatcgccggg ctgcggtccc ggcattgtgc ggttgagcgc gaggaaatcg
     1441 ccggtctcgc agactggccc gacgacatcg gcgaggattt ccggcgcgcc gggccgaggc
     1501 tgcaccggaa tgatctcatg atgcgcgtca taaagcgtcg gccggatcaa atcgttcatg
     1561 ccggcgtcga cgatgacgaa ggtcttggcc tcggcctttt tcacgtagag aacgcgggtg
     1621 acgagaatgc cggcattgcc ggcgatgagg cggcccggct cgaaaatcaa ttgcttgccg
     1681 aggccgccga aatgacgccg cacgatcttg gcgtaaagat cggggtgaaa ggcatcgccc
     1741 ttatcgtccg gccggtaggg tatgccgagg ccgccgccga gatcgatatg ctcgatctta
     1801 tggccatcgg ccgccagcac gttcaaaaac gccgccacca aagcgaaggc attgtcgaac
     1861 ggctcgaggt ccgtgatctg cgaaccgata tgaatatcga tccccgtgac ttcgatcccc
     1921 ggcagcgccg cggcgtgagc atagacctcg cgcgcccgcg cgagcggaat accaaacttg
     1981 ttctccgcct tgccggtcga aatcttcgca tgggtgcgcg catcgacatc cggattgacg
     2041 cgcagtgcga tgcgcgccgt gcggccccgc gccgaagcaa tttgcgagat cgccgcaagc
     2101 tcgggttccg actcgacgtt gaagcacatg atgtcttcgt cgagcgcggt gcttatctcg
     2161 acggcggtct tgccgacgcc ggaaaaggtg atcttcgccg ccgggatgcc cgcggcgcga
     2221 gcccggcgca gttcaccttc cgagacgaca tccatgccgg ctcccaattg gccgagcgtc
     2281 ttcagcacgg cctggttgga attggccttc accgcatagc agacgagaac tggtaattcg
     2341 ccgaacgagc gtttgaagac ctcgtaatgg cgggtgagcg tcgctgtcga atagcaatag
     2401 aatggcgttg cgacctctgc tgccagacgg tcgatattga ccccttcggc gtggagaacg
     2461 ccgtccttca gcgcgaaatg gtgcattcgc cggctatttc actaagggat cgaggagaaa
     2521 ggtattgccg ctcacggcct gcgtcccgct gttcgcgggc gtcggctttc tgccgggctg
     2581 cgccggatcg cccgccatgg gatcgatatt cttggtctgc atcggcacga cctggctcgc
     2641 tggcgtattt ggcggcggct caagatcacc gcgcctgccg cagccggcga gcgccagtgc
     2701 gagaaagacg aggccgaaga cgccgcatcg acggcaggaa gaaggattca cacaacagct
     2761 ccgctaagcc tcggccttat aaaccaagcc tttggcaaaa cgaagccgcc ggcctgcacc
     2821 gcccttaatt ccccggcttt ttcaggcgat cgagccaagc ttgtgcctgc tttgccacgt
     2881 tcgcgggcgc cgtgccgccg tagctcgtgc ggctcgcgac cgaattctcc acgccaagca
     2941 ccgcaaacac atcctcgccg agccgcggtt cgaccttttg catatcggca aggctcaagg
     3001 cttcaaggcc aatgccacgt tcggccgcca aagccaccaa atggccggtg acatgatgcg
     3061 cctcgcgaaa cggcagtccg agttcgcgga cgagataatc ggcaaggtcc gtcgccgtcg
     3121 catagcccgc acccgccgcc gctttcatcc gcgcgagatc cggcgcgaaa tcgccgacca
     3181 tgcctgtcat cgccgcaagg cagagcgaca aggtctggag cgcgtcgaac gtgccttcct
     3241 tgtcctcctg catatccttg gaataggcga gcggcaggcc tttgagcacc accagcaggc
     3301 cgctgagcgc gccgatcacc cgcccgctct tgccgcgcac gagttcggcg gcatccggat
     3361 tgcgcttctg cggcatgatc gacgagccgg tcgagaacct gtcggagagc cgcaggaagc
     3421 cgaattgcgg cgtcgtccag agcacaatct cttcagcaaa acgcgaaaga tgcacagcgc
     3481 aaagcgccgc ggc
//
LOCUS       DASWOG010000018         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147094,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000018 DASWOG010000000
VERSION     DASWOG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147094"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="bamD"
                     /locus_tag="VGB93_00320"
     CDS             complement(<1..556)
                     /gene="bamD"
                     /locus_tag="VGB93_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0144605.1"
                     /translation="MPVALVPQRPVRRHGLRIGLQALGILMLTLPIAACSNSLDSLGS
                     FNPMNMFSGEKYETKVVPDTPAETLYNQGLVDINSHDYDKATKKYSALEKQYGFSQWE
                     RKALLMETFTQYQNGNYDDAIGTAQRYIGRYISAPDLDYVYYLEAMSYYNQIPDVSRD
                     QDRAKKAAIVFQQLIDKFPKSQYAD"
     gene            complement(933..2603)
                     /gene="ftsZ"
                     /locus_tag="VGB93_00325"
     CDS             complement(933..2603)
                     /gene="ftsZ"
                     /locus_tag="VGB93_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0144606.1"
                     /translation="MSIDLKAPELRELKPRIMVCGIGGAGGNAVNNMIVSGLIGVDFI
                     VANTDAQALMSSRAERIMQMGMQVTEGLGAGSQPEVGRAAAEEVIEEIRDHLAGAHMC
                     FVTAGMGGGTGTGAAPVIARVARELGILTVGVVTKPFQFEGVRRMRVADAGILELQKS
                     VDTLIVIPNQNLFRIANEKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVR
                     AVMREMGKAMMGTGEATGEKRAILAAEAAIANPLLDEVSMKGARGLLISITGGNDLTL
                     YEVDEAASRIRQEVDEDANIILGATFDQNLDGMVRVSVVATGIDSEDMRAFNPIETRI
                     AEVTERPRIRTEVRTSEILAERRAAAEAPAPRFEEPVTYAEPAPLAQPATTRGVHLQP
                     TRAPAPQYVEPEHAQLPEPEPFDAGPFIPPAAESPVVRAQRMPQIEDLPMPAQKQIRA
                     QHAEPIDTPHRKTLLERLAAFGVSRHDEPPRQVNVPVREPVARIAAPAPRHAQPGNVH
                     AEYGKRPAQSTPRQVQSASDAHQGRAPAPRSIEDDQLEIPAFLRRQSN"
BASE COUNT          529 a          807 c          833 g          566 t
ORIGIN      
        1 cgtcggcata ttgcgatttc ggaaatttgt cgatgagctg ctggaagacg atcgccgcct
       61 tcttggcgcg atcctgatcg cggctcacgt ccggaatctg attataatag gacatggcct
      121 cgagataata aacgtagtcg agatcgggag ccgaaatata acggccgatg taacgctgcg
      181 ccgtaccgat cgcatcgtcg taattgccgt tttggtattg cgtgaacgtc tccatgagga
      241 gcgctttgcg ttcccattgc gagaacccgt attgcttctc gagcgccgaa tatttcttag
      301 tcgccttgtc gtaatcgtgc gaattgatgt cgacgagccc ctgattatag agcgtctcag
      361 ccggcgtatc agggacgacc ttggtctcat atttttcgcc gctgaacatg ttcatcgggt
      421 tgaacgaacc gagcgagtcg agcgagttcg aacaggccgc gatcggcaac gtgagcatca
      481 aaatgccgag ggcttgcagg ccaatgcgca ggccatgacg gcgaacgggg cgttgcggaa
      541 ccaaggctac aggcatgttg gacaactcga ccatggctcg acgaaggacg gggccaggtg
      601 tgcgacagta tccgcttaac cccagccatc agctaactcc tcggctggat aagaggcatt
      661 tctgtggcaa atgcaaccca ctcaggtctc tcagtgtcct gggcgcggcg gcaaatcatg
      721 gctcaaacgt ccaccgccga tggctcgcag gaatttcgtg gccgatctgc cacgccgctt
      781 acatttatgc tgtcgatgca cctgcgaaat gtgtgcggtg cgtcggatga aacgggaaga
      841 gaagagcggc gtgcgcaaat gcgaactcat atgcgcaact agccgggcgc gatgcccggc
      901 taggaagatt atagaacaga cacttccgtg gatcagttcg attggcggcg caggaacgcc
      961 gggatctcca attgatcgtc ttcgatcgag cgcggtgccg gcgcacggcc ttgatgggca
     1021 tcgctcgcgg actgaacttg ccgcggcgtg gactgcgcgg gccgcttgcc atattcggca
     1081 tgaacgttac ccggttgagc gtggcgcgga gcgggcgccg cgatccgcgc caccggctcg
     1141 cgcactggaa cgttcacctg acgcggcggt tcatcgtgcc ggctgacgcc aaaagctgcc
     1201 aagcgttcca gcagggtttt gcgatgcggc gtatcgatcg gctcggcgtg ctgggcgcga
     1261 atctgctttt gtgccggcat gggcagatcc tcgatctgcg gcatgcgttg ggcacggacg
     1321 accggcgatt ctgctgccgg gggaatgaac gggccggcat cgaagggttc cggctccggc
     1381 aattgcgcat gctcgggctc gacatattgc ggcgcaggcg cgcgcgtggg ctgcagatgc
     1441 acgccgcggg tcgtcgccgg ctgggctagg ggcgccggct ctgcataagt cacgggctcc
     1501 tcgaaacgcg gcgcaggcgc ttctgccgca gcacgacgct ccgccagaat ttcactcgtg
     1561 cgaacttcgg tgcgaatccg cggccgttct gtcacttccg caatccgcgt ttcaatgggg
     1621 ttgaacgccc tcatgtcttc gctgtcgatg cccgttgcga cgaccgaaac ccggaccata
     1681 ccgtcgagat tctggtcgaa ggtcgcgccg agaataatgt tggcgtcttc gtcgacttcc
     1741 tggcggatgc ggctcgcggc ctcatcgact tcgtaaagcg tgagatcatt gccgccggtg
     1801 atcgagatca gcaggccgcg cgcgcccttc atcgagactt cgtcgaggag ggggttggcg
     1861 atagcagctt cggccgcgag aatcgcgcgc ttctcgcccg ttgcctcgcc ggtgcccatc
     1921 atggccttgc ccatttcgcg catcacggcg cgaacgtcgg cgaaatcgag attgatcagg
     1981 ccttctttca ccatcaggtc ggtgatgcaa gcgacgcccg aataaagaac ctgatcggcc
     2041 atcgcgaagg catcggcgaa cgtggtcttt tcattggcga tgcggaacag attctgattg
     2101 gggataacaa tcagcgtatc gaccgacttc tgcaattcga gaataccggc atcggcgacg
     2161 cgcatgcgcc gcacgccttc gaactggaag ggcttggtga cgacacccac cgtcagaatg
     2221 ccaagttcgc gcgccacgcg tgcgatcacc ggggcggccc ccgtaccagt gccgccgccc
     2281 atgccggctg tgacgaagca catatgtgca ccagcgaggt gatcgcggat ttcttcgatg
     2341 acctcttcag ccgcggcgcg gccgacttct ggctgggagc cagcgccgag accttccgtg
     2401 acctgcatgc ccatctgcat gatacgctcg gcgcgagatg acatcaaagc ttgggcgtcg
     2461 gtattcgcga cgatgaagtc gacgccgatg aggcccgaaa cgatcatgtt attgacggca
     2521 ttgccccccg cgccgccgat gccgcacacc atgatgcggg gcttcagttc tctcagctct
     2581 ggagccttaa gatcgatcga cattttttgg cctcatgacg ttggagcgct ttaagagcgc
     2641 ccgcttgtta tcccattacg ccgaagaacg aatcagccgc cgcgcttaat cgttcttctt
     2701 cataccaact aaaaactttc tctcaaccat tgccc
//
LOCUS       DASWOG010000019         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_55468,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000019 DASWOG010000000
VERSION     DASWOG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55468"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VGB93_00330"
     CDS             <1..681
                     /locus_tag="VGB93_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0144607.1"
                     /translation="MSIGGPQPLDKAHLEKFRSLVERNDPVLVSEHLAWSTHETTFFN
                     DLLPLPYTEATLARVSDHIDEVQEAIRRPILLENPSTYVSFHESTMSETDFIRAIAQR
                     TGCGLLLDVNNVFVSATNHGFSALEYLSDFPLARVGEIHLAGHAEQSDDEGDLLLIDS
                     HDGPVADAVWRMFDIVIGRCGPVPTLVEWDSNIPDWPALRAEAIAAQAILDRYKTIVL
                     PDGRHAAA"
     gene            668..1474
                     /locus_tag="VGB93_00335"
     CDS             668..1474
                     /locus_tag="VGB93_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY0144608.1"
                     /translation="MPPLEDACRNIESGPSFAAEFSPALINPERETPDAVTGPNGKLA
                     IKRFNVYRNNVTVSLIDALASIYPAVQRITGVDFFRAMARFHIRATPPHSPLLFEYGR
                     DFPAFIERYQYAQSLPYLGDVARVERAWLDAYHAADASPLAPEALAAVSPERLADVVF
                     IPHPATRIVRSQFSAVTIFAANRTDGPIGLIESAVPEDALITRPDLEVVVRHLPPGGA
                     IFLTHLVAGETLGAAVAATLEIEPSFDIPASISGMIEAGAFTTIDFGDEQ"
     gene            1477..1992
                     /locus_tag="VGB93_00340"
     CDS             1477..1992
                     /locus_tag="VGB93_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0144609.1"
                     /translation="MTNNRGAVRERIRPVSILVEKADALVRVIAQPSFVQLVTRIALA
                     VPFWRSGILKWSGFLQLNDTAVYLFSDEFKLHLPGGPYSFPMPADFAFLSGCGEIIFP
                     VLLVLGFATRFAALGLLVMTLIVELTVPDGWPVHITWAAMALSIMAWGPGRLSVDAGL
                     KYVFGTRETSK"
     gene            complement(2022..2513)
                     /locus_tag="VGB93_00345"
     CDS             complement(2022..2513)
                     /locus_tag="VGB93_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0144610.1"
                     /translation="MKITLACSALLTMFATTLTVPAAVFAEDSPNVSIGTLFIEGAFT
                     RPTKAGAKDAEGYLSIINEGSVPDRLVSVDSDIANKAELRASGADGQTLSDGIEIPAG
                     ATVNLAPGGERVTFLNIHHPLKDGDIIHATLGFENAGQIDIGFRAIPSLSSLMSSGDS
                     KKK"
     gene            complement(2621..>3190)
                     /locus_tag="VGB93_00350"
     CDS             complement(2621..>3190)
                     /locus_tag="VGB93_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEY0144611.1"
                     /translation="VVIDTPASDSYLMRLAHSLADTLVSPVNDSFIDVDVFSRVHHDR
                     SQRGQVAHYADLVLEARRKRKLVDQGIIDWIIVRNRMSSTQSNNAKQISASLSRLAPE
                     LQFRPADGLHDRVIFRELFPIGLTTLDPIEEAMRGSSLTNSQLAARREVETLIATLQL
                     PERSRGMAHLQARHDWFERISRYYRDLKG"
BASE COUNT          600 a          948 c          940 g          702 t
ORIGIN      
        1 atgtcgatcg gtgggccgca gccgctcgat aaagctcatc ttgaaaagtt ccggtctctg
       61 gttgaacgta atgatcccgt actcgtttca gaacatcttg cttggtcgac gcatgagacg
      121 acctttttca atgatcttct gccgttgcct tacaccgaag caacactcgc gcgcgtcagt
      181 gatcatattg acgaggtgca agaggcgatc cgccgtccca tcctgcttga aaacccgtca
      241 acctacgtct cgtttcacga gtcgacgatg agcgaaaccg acttcattcg cgccatcgct
      301 cagcgtaccg gatgcgggct gttgctcgac gtcaacaatg tcttcgtgtc ggcgaccaat
      361 cacggcttct cggcgcttga atatctctcc gactttccgc tcgcccgtgt cggcgagatc
      421 catttggcag gtcacgccga acagagcgat gatgaaggcg accttctgct catcgacagc
      481 cacgatggcc ccgtcgccga cgcagtgtgg agaatgttcg atatcgtgat cggccggtgc
      541 ggacctgttc cgacactcgt cgaatgggac agcaatatcc ctgactggcc ggcgctcagg
      601 gccgaagcca ttgccgccca agcgatcctc gaccgctaca agactattgt tctgccggat
      661 ggccgccatg ccgccgcttg aagatgcgtg ccgtaacatt gaaagcggcc cgtcgttcgc
      721 cgcggaattt tcgccggcgc tgatcaatcc cgaacgggaa acgcctgatg ccgtcaccgg
      781 gccgaatggc aagctcgcaa tcaagcgctt caacgtctat cgcaacaatg tcaccgtcag
      841 tctcattgac gcgctggcgt cgatctatcc tgccgtccag cgcatcaccg gcgtggattt
      901 cttccgcgcc atggcacgct ttcatatccg cgccacgccc ccgcattcgc cgttgctgtt
      961 cgaatacggg cgcgattttc cggccttcat cgaacgctac caatatgcgc aatcgctgcc
     1021 gtatctcggg gacgtggcgc gggtcgaacg ggcctggctc gacgcttatc atgcggcgga
     1081 cgcttcaccg ctcgcgcccg aagctttggc cgcagtctcc cccgagcgat tggccgacgt
     1141 cgtcttcatc cctcatcccg cgacgcggat cgtgcgctcc caattttccg cggtgacgat
     1201 tttcgccgcg aatcggacag atggaccaat cggcctgatc gaatccgccg tgccggaaga
     1261 tgcactgatc acgaggccgg atctcgaggt cgttgtccgg catctgcctc cgggtggcgc
     1321 cattttcctg acgcatcttg ttgccggcga aacgcttggc gctgcggtgg cggcgactct
     1381 tgagatcgag ccgtcgttcg acattccggc aagcatcagc gggatgatcg aggccggcgc
     1441 cttcacgaca atcgattttg gagatgagca atgacgatga cgaataatcg cggcgcagtc
     1501 cgcgaaagaa ttcggccggt ttccattctc gtcgaaaaag cagacgcgct ggtgcgcgta
     1561 attgcccagc cgtcctttgt gcaactcgtg acgcgtatcg cgcttgccgt gccgttctgg
     1621 cgttccggca ttctgaaatg gagcggcttt ttgcaattga acgacaccgc tgtttatctc
     1681 ttcagcgatg agttcaaatt gcatctaccc ggtgggcctt attcgtttcc gatgccggct
     1741 gatttcgcct ttctgtctgg gtgcggcgaa atcattttcc ctgttttgct cgtgctcggc
     1801 ttcgcaacac gcttcgcggc gctcggtttg ctcgtcatga ccttgattgt cgaactcacc
     1861 gtccccgatg gatggcctgt tcacatcacc tgggcagcga tggcgctgag catcatggcc
     1921 tggggaccgg gcagactctc cgtcgatgcg ggtctcaagt acgttttcgg aacccgtgag
     1981 acttcgaagt agcagagcgt tcccttccgg ccgtcagcag cctatttctt tttggaatcg
     2041 ccgctcgaca tcaacgaact caggcttggt atggcgcgaa agccgatgtc gatctggccg
     2101 gcgttctcga agccgagggt cgcgtggatg atgtcgccat ccttcaacgg gtgatgaatg
     2161 ttcaaaaacg tcacgcgttc gccgccggga gcaaggttca cggtcgcgcc ggccggaatt
     2221 tcgatgccat ccgacaaggt ctgaccgtcc gcgcctgagg cgcgcagctc ggccttattg
     2281 gcaatgtcgg aatcgacgga tacgagccga tccggcacgc tgccttcgtt gataatcgaa
     2341 agatagccct cggcgtcttt cgcgccggcc ttggtcggcc gcgtgaatgc gccttcgatg
     2401 aacaaagtgc cgatgctgac gttgggcgaa tcttcggcga agacggcggc ggggacagtt
     2461 agggtcgtcg cgaacatcgt aagaagggcc gagcaggcga gggtgatctt caagttttgc
     2521 tccgttattc tgggcgtcgg ccctgccgcg acggccggcc ctattctcac cttcgcagga
     2581 acgaaggctt tgcgttttta tcgtgacaag ccgggcgacc ttagcccttg agatcgcgat
     2641 aatagcggga aatccgctca aaccaatcat gccgggcctg aagatgggcc atgccgcggc
     2701 tgcgctccgg taattgcagg gtcgcgatca gcgtttcgac ctcgcgccgg gcggcaagtt
     2761 gcgaatttgt caggctggag ccgcgcatcg cctcctcgat cggatcgagc gtcgtgaggc
     2821 cgatggggaa aagttcgcga aaaatcacgc ggtcgtgaag cccgtcagcg ggtcgaaatt
     2881 gcaattcagg cgcgaggcgc gacaggctcg cggaaatctg cttggcattg ttcgattggg
     2941 tcgaggacat gcgattgcgc acgatgatcc agtcgataat gccctgatcg acgagtttgc
     3001 gcttgcgccg cgcttcgagc accaggtcgg cataatgggc gacctgacca cgttgtgagc
     3061 gatcatgatg cacgcgcgag aaaacatcga cgtcgatgaa ggaatcgttg accggcgaaa
     3121 cgagcgtatc ggccagcgaa tgggcgagcc gcatgagata ggaatcgctt gccggcgtgt
     3181 cgatcacgac
//
LOCUS       DASWOG010000020         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_293793,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000020 DASWOG010000000
VERSION     DASWOG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293793"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..481
                     /locus_tag="VGB93_00355"
     CDS             110..481
                     /locus_tag="VGB93_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144612.1"
                     /translation="MDVAAELRMMNVADYVSFIHLEQFNNIRDIVNSSMELYFKHGTL
                     SYGCDADYELEWDQPPSIVLDFEFRHQEITVRFNLTLQAAHASVKISEISFATGSHGE
                     AADTRRLIAAIADAKLASRNC"
     gene            553..936
                     /gene="flgB"
                     /locus_tag="VGB93_00360"
     CDS             553..936
                     /gene="flgB"
                     /locus_tag="VGB93_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY0144613.1"
                     /translation="MEPVYLFDLASAQQHWLAVRQATVAENVANANTPGYVGKDVAPF
                     VDVLQQAQLQLNATQPGHISSDPLSTDDSAKDGSPWEVVPSGNSVSMEHEMLKANSVG
                     RDYSMNAAIVKAFSQMFAISVKASS"
     gene            933..1355
                     /gene="flgC"
                     /locus_tag="VGB93_00365"
     CDS             933..1355
                     /gene="flgC"
                     /locus_tag="VGB93_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY0144614.1"
                     /translation="MIDPIEAAIDVATSGLEAHSARMRVISENLANSESTGLTSGANP
                     YTRKTVTFTDEMDQATGVDMVKVGSIGYDRSPYRVEYDPGNPAADASGFIKLPNVNLI
                     TEMADMREANRSYSANIQVVKQARELYTMTVDLMRNSQ"
     gene            1352..1678
                     /locus_tag="VGB93_00370"
     CDS             1352..1678
                     /locus_tag="VGB93_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY0144615.1"
                     /translation="MIPAISLLGTVAADAIGSISSIAPAITGGGAAVGAPPADFSSML
                     AQVTGDAIDKLKSGEATAISGIQGKASIQDVTKSVMDAQQSLQTVAAVRDKVVSAYQE
                     VSRMAI"
     gene            1689..>2006
                     /locus_tag="VGB93_00375"
     CDS             1689..>2006
                     /locus_tag="VGB93_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY0144616.1"
                     /translation="MSSTVLAIAATGMAAQSLNVEVIANNIANMNTTGFRGSRAEFTD
                     LIYQPERLPGVSSRGQNSAVPEGAMVGLGVRTAAVRALQIQGTLNSTGNPLDVAVSGR
                     GWFQ"
BASE COUNT          428 a          563 c          591 g          424 t
ORIGIN      
        1 cagagacacg cgcgccgccg ggcgccgata cattgaaagt acgctaatgc tagcagattc
       61 gcgcggtgat caatcactct tgcgggctaa agccatcgca gaggggatca tggacgtggc
      121 ggccgaactt cggatgatga acgttgccga ttatgtttcc ttcatccatc tggaacagtt
      181 caacaatatc cgcgatatcg tgaactcctc gatggagctt tatttcaagc atgggacgtt
      241 atcctacggc tgtgacgccg attacgagct cgagtgggat cagcctccgt cgattgttct
      301 cgatttcgag tttcgccatc aggaaatcac cgtccgcttc aatctgacct tgcaggcagc
      361 ccacgcgagc gtcaaaattt cggagatcag cttcgcgaca ggctcgcacg gcgaagccgc
      421 cgatacgcgc cggctcattg ccgccatcgc cgatgcgaag ctcgcatcga ggaattgctg
      481 aaacgcaagc gcgccagcct ggggcaagat ttagcccgta aatttcctat ggcttgagag
      541 gcggatacga ccttggaacc cgtttacctt tttgatctcg cgtcggcgca acagcattgg
      601 cttgcggtgc gccaggcgac ggtcgccgaa aacgtcgcga atgccaatac gccgggctat
      661 gtcggcaagg acgtcgcacc gttcgtggat gtgcttcaac aggcgcagct gcagctcaat
      721 gcgacccagc ccggccatat atcgtccgat ccgctcagca ccgacgattc tgccaaggat
      781 ggttcgccgt gggaagtggt gccgtcgggc aattcggtga gcatggagca tgaaatgctc
      841 aaggccaatt cggtcggccg cgattattcg atgaatgcgg ccattgtgaa ggccttttcg
      901 cagatgttcg cgatcagtgt gaaggcttcg tcatgatcga tccaattgaa gcggcaatag
      961 acgtcgcaac ctcggggctc gaagcccatt ccgcgcgtat gcgcgtgatc tccgagaacc
     1021 ttgccaattc ggaatctaca ggtttgacgt cgggtgccaa tccctacacg cgcaagacgg
     1081 tcactttcac ggatgagatg gatcaggcaa cgggcgtcga catggtgaag gtcggttcca
     1141 ttggctatga ccgttcgccg tatcgtgtcg agtatgatcc tggcaatccg gccgctgacg
     1201 cgagcggctt catcaagttg ccgaacgtca atctcattac cgagatggcg gatatgcgtg
     1261 aggcgaaccg ttcctattcc gccaatatcc aggtggttaa gcaggcccgc gaactttaca
     1321 cgatgacggt cgatctcatg aggaattcgc aatgatcccg gcaatctcgc ttcttggtac
     1381 cgtcgcagcc gacgcgatcg gcagtatttc atctatcgcc ccggcgatca ccggcggggg
     1441 ggcggctgtg ggcgcgccgc cggccgattt cagctcgatg ctcgcacagg tcacgggcga
     1501 cgccatcgac aagctgaaga gtggcgaagc gacggccatt tccggcattc agggcaaggc
     1561 ctccatacag gacgtgacca aatcagtgat ggacgcgcag caatcgctgc aaactgtggc
     1621 tgcggtccgc gataaagtcg tctcggccta tcaagaagta agccgtatgg cgatctgagg
     1681 gacataaaat gtcatcaacg gttctggcga tcgcggcaac gggcatggcg gcccaaagtc
     1741 tcaatgtcga agtcatcgcc aataatattg ctaatatgaa cacgaccggt ttccgcggtt
     1801 cacgcgcgga atttaccgat ctcatctacc agcctgagcg gctccccggt gtgtcgagtc
     1861 gagggcaaaa cagcgcagtg cccgagggcg ctatggtcgg tctcggtgtc cggactgcgg
     1921 ccgtgcgtgc cctgcagatt caaggcacgc tcaatagtac gggcaatccg cttgatgtcg
     1981 cggttagtgg tcgcggctgg tttcag
//
LOCUS       DASWOG010000021         9579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147121,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000021 DASWOG010000000
VERSION     DASWOG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147121"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..992
                     /locus_tag="VGB93_00380"
     CDS             312..992
                     /locus_tag="VGB93_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEY0144617.1"
                     /translation="MISHKYRCIFIHIQRTAGSSIEEMIATEDWWHREASTKHLLASQ
                     AKRLYADYWDDYFKFSFVRHPVTRIMSCLKFADHFGLSLSKGNRINFDGYRGVFGDRI
                     VVEFDHRFYRREEIIQPQHCEGSVYGNILDEELDFIGKFENLAEDTKFISDRLGIPYM
                     KLPHLERSRTSSIRPNNHVLADIETLYARDFERFDYSLLDTQLLRERQSFLDRFSNRA
                     STLALSRA"
     gene            1245..1868
                     /locus_tag="VGB93_00385"
     CDS             1245..1868
                     /locus_tag="VGB93_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0144618.1"
                     /translation="MRRYHWLGKYDRDTINSIIDAGIICQVGYVFNGEPYVTPTSHWR
                     IDDYVYWHGSSASRMLKTQQTGIPVCFTVTHLDGIIFARAAMNHNVQFRSVMAFGNAE
                     LVDEDKKREVLAVFTNKLAPGLWDYARPPSEQEWLATKVIRMRLEDVSAKINDVFAGD
                     EEEDLASDRWSGAVPLYVGQGEIIPDPRLAPGIEMPDFAQLFRYTRK"
     gene            1936..3426
                     /locus_tag="VGB93_00390"
     CDS             1936..3426
                     /locus_tag="VGB93_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0144619.1"
                     /translation="MLIELHLSRAAGPTLQAQIADQLRRQILSGRLQSDYELPPSREL
                     ARQYGISRNTIIRAYEHLISEGYLATVKGVRTVVAESIPEACLSVGKEVAPPNNQYFA
                     PRAPVVFAGENLAVPAAARRTSKLIDFWPGRANVAHFPAATWRKLADDVLSAQSRELV
                     EYGDPAGLPALREAIAQHVANSRGVRCSAEAVIITAGTQEAINLVARMLVAGNTGVVI
                     EDPGYGSAAHTFRSYGAHMHTIPVDRDGLVTDLLPKEGISFAYVTPSHQFPTGATMSA
                     PRRRALLDWAYRTGAYIVEDDYDSDYSFDGPPALSLAGADDGQCVIYVGTFSKSLGAG
                     VRTGYLIVPPQLIDVARRAKAMNNYGHPWLEQAILQGFISTGAYQRHLRTIRKASSDT
                     IGFLISCLQASFGQLDIWGVNGGMHVMWLLPDWMPPAEDFKGRLEPQGVHVHTLVSGG
                     AYCQTGLYCDRAILLGYAALTQRQIGRAVEIMQRVIPSGYASWRQA"
     gene            3590..4597
                     /locus_tag="VGB93_00395"
     CDS             3590..4597
                     /locus_tag="VGB93_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0144620.1"
                     /translation="MLRLNDKDWFQRLGQVAKSIGSREFHHELVDLFGASIRHDSCWI
                     IRFSSTATPEVLFTNNVADDVVHTYSKSYSELDPFSDYWRNFERAGVTMLSQARAQSK
                     RTTAYSQVFLPQAHISDEMSIFLPTVGRDCFGLFLERENGQFSKADVERARLVFPALE
                     GCHRAHIAWVFNNLRDVEGMELNGFANKPILIQDRFGAEVYSNDAWNEARVGVALDEL
                     VDAFLVQDGGHARSFDDFVLKYEPLDRDFPLAPGGRMFVLETNAAAAQSGQAFADARA
                     LFTPREYDVFSLMMEGRTTGQIAQALEISKGVIKNYKLRIYRKAEVTSERALVRRFAL
                     V"
     gene            complement(4589..5725)
                     /locus_tag="VGB93_00400"
     CDS             complement(4589..5725)
                     /locus_tag="VGB93_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY0144621.1"
                     /translation="MNTHVYSRQSVLNDRHRALGTQFEASWNDMPIPQLYATDPYFET
                     EAVRYRAGLFDVTSLRLIDATGKDALMALNELLTSNITKLAPGKSAISNIVDENGSLI
                     DDVLVYCIAPDRYRISHGGGALEDVLPSVTQGRDVQFRKDNDTHILSLQGPKALDILA
                     PHTPLKLADLGYFEHAPTTLFGHKVTLARGGYSGERGYEVFCRAADAVFLWDSILEAG
                     KPFGAIPVSWACLDIVRVESALLFFPFDMPEGDTTPWEVGVDWTVDLDKPSFRGKEAL
                     IRRRGEKRIAQVGIEIDHDKAIEPGAKLFKGGEVVGIVNSTTYSEHLMRSIALVHVAP
                     AFADYGMEMQVRSEAGTFPARVVKVPFYDPLRLRTHPVAERQSD"
     gene            5944..6528
                     /locus_tag="VGB93_00405"
     CDS             5944..6528
                     /locus_tag="VGB93_00405"
                     /inference="COORDINATES: protein motif:HMM:NF041259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylamine monooxygenase subunit DmmA family
                     protein"
                     /protein_id="HEY0144622.1"
                     /translation="MPVPTISSRPRYQPFELIPVAGSHLVVAETRQAPRADIPKSWAD
                     APIEFWNVAAESAIFDAAIAQDGILQRGFRSASHLLDRLSYRLEHEHVGLHLYIIGTE
                     PFIWDVVKLARSFGMDSDEYHVTHEGSEKRRVYCVHCRTMTQDVTTNIATCTGCGAHL
                     QVRDHFSRRLAAFIGVQIDAEVPGDIPPIEEAFP"
     gene            6525..7475
                     /locus_tag="VGB93_00410"
     CDS             6525..7475
                     /locus_tag="VGB93_00410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY0144623.1"
                     /translation="MKAAKLNLRVRASETIAPSLRSFTLEAADEGELPARGPGAHLRL
                     MLKDGSQAWRNAYSIAAASTDRREYRIIVRRVAHSRGGSAFLHEAVRPGQLIEADFPG
                     NLFPIVQIARKHLLVSGGIGITPFLSYLGHLKALGTPFELHHFCRAEEVAIFENLLAE
                     YDSSRIFIHPEATQFDLVETLRTQPLGTHLYVCGPAELMDLVIAIARAHGWPASKYHS
                     ESFGGVHAGGAPFIAVLQRAGIEVRVGQDQTLLEALEAAGFEPSCLCRGGACGECRTE
                     VIEGTPEHRDHYLDADERATNRAMMICVSRAVGDRIVLDL"
     gene            7489..8544
                     /locus_tag="VGB93_00415"
     CDS             7489..8544
                     /locus_tag="VGB93_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HEY0144624.1"
                     /translation="MTIAFKVAETFRDDFSYRNSEAAILRFPFPFPEDKYMYSVNIEP
                     HVAGGSSPAYDHAFDVDEHYIAECRERALVLAQDPGRCKVLPHMMTAQWDTLELIMES
                     LARDYPEHFSLAKNGTVWTWKNRPLGITQTFTFGDASTLPSEPFEYITRQAQGDFTLQ
                     DQRDNNLFVDGGMVTAQADWSLEFDVGMAFHEWHAPVPLAHQIGVFDRALKFLLRLQQ
                     GQPVRRLNWTMTVNPRLDTSPENYPLWAPDRLTVTPENIAEKVNVRVELQTLFRLPRS
                     NAILFGIRCYLASIEELGRVVKWRKRLHRVLRDLPPEIETYKGLTRYRQMAVDYLAPF
                     DDGAPLSKGSQADQNAL"
     gene            8989..>9579
                     /locus_tag="VGB93_00420"
     CDS             8989..>9579
                     /locus_tag="VGB93_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0144625.1"
                     /translation="MAELRRNNLTLVEAVGQSVCSLSPTFTPALGVAVVGGMAGANSW
                     LVYLLATISVTIVGINIGKLAKRIPAAGSFFLYVSRTLGPSWGLLSGWAMLVAYLFTA
                     VALTVATSIFFKDFFGAIGFKTLPPDWLIYMAVSALIFTLAYRDVKLSTRFGLTLEAL
                     SVAAIIVVCFLIWKSFGFKIDAPQVHLQGASFGTIAP"
BASE COUNT         1897 a         2824 c         2747 g         2111 t
ORIGIN      
        1 cagggcgagc aagcaagtgg cgctcagcgt cgcccgcgac ctgaaacaat cgctttaagc
       61 ccatctcttg ataaacagat tgcgcgatca catcgcgcaa tctgctaaat cacgggagcg
      121 tgaaatcgtg atgcggatgg ccaatttccc gcgtcgcaat aatgatttgc gccgcacgtt
      181 gcttgaactt ccagctttac aacttttggt gaatcacagg gatgtcaatg gattgatttt
      241 tcgacgcgtt taattgtggt ggttgcggtt ttctgtcgcg ttttgcgatt tgaaggggac
      301 actccgctgc gatgatcagc cataaatatc gatgcatttt cattcatatc cagcgaaccg
      361 ctggatcaag cattgaggaa atgatcgcta cggaagattg gtggcaccgc gaagcatcga
      421 ccaaacatct gctcgcgtct caggctaagc ggctctacgc cgactattgg gacgattatt
      481 tcaagttttc cttcgtgcgc cacccggtga cacgcatcat gtcctgtttg aagttcgctg
      541 accatttcgg gctctcgctc tccaaaggta atcgtatcaa tttcgacggt tatcggggag
      601 tttttggtga cagaatcgtc gtcgagttcg accaccgctt ttatcgtcgc gaagaaatta
      661 tccagccgca gcattgcgaa ggctcggttt acggaaatat cctggacgag gaattggatt
      721 tcatcggcaa gtttgaaaac ttggccgagg acaccaaatt cattagcgac cggctgggaa
      781 ttccctacat gaagctgccg catctggagc gtagccggac gtcgtcgata aggccaaaca
      841 accacgttct tgcggacatt gaaacgcttt acgcgcggga tttcgaacgt ttcgattata
      901 gcttgctgga tacgcaacta ctgcgcgaac ggcagagttt tctggataga ttttccaacc
      961 gcgccagcac actggctctt agccgcgcgt gacgcctcgc aaaatttagc cccgggttcc
     1021 gggcggcagg tcatcatgaa tctcatgcgc tgcacgaggc cttgcatcct ggacccatga
     1081 atttctcgca gcgcggttct tccaactcat ctgatatcgc gacagcatga acttcagtga
     1141 agttcacgaa gtgtcgcccg gcgccgaggc tccggcggcg agcaagcgat tagaggtcgg
     1201 caataatggg cgaacccgct cttccatttt cggatcggac gcggttgcgc cggtatcatt
     1261 ggctcggcaa atacgaccgg gacacgatca atagcatcat cgatgccggc atcatttgtc
     1321 aggtgggcta cgtttttaac ggtgaaccct atgtgacgcc aacaagccat tggcgcatcg
     1381 acgattatgt ctattggcac ggctcgtcgg cgagtcggat gctcaagacg caacagaccg
     1441 gcatcccggt ctgcttcacc gttacccatc tggacgggat catttttgcg cgcgcggcaa
     1501 tgaaccataa tgtgcaattc cgctccgtca tggcctttgg gaatgccgaa ctggttgacg
     1561 aggataaaaa gcgcgaggtg ctcgccgtct tcaccaataa gctcgcgccg gggttgtggg
     1621 actatgcgcg gccgccgagc gagcaggaat ggctggcgac gaaagtcatc cgcatgcggc
     1681 tcgaagacgt atcggcaaag atcaacgatg tcttcgccgg cgacgaggag gaggacctgg
     1741 cctcggatcg ctggtccggc gcggtgccgc tttacgtcgg acagggcgaa atcattcccg
     1801 atccgcggct tgcacccgga atagaaatgc ccgatttcgc gcagctcttc cgctatacgc
     1861 gaaaatagtc tcgaaccgga cgggcacgat ccttgcgtca aaggtcccta cagcctttgg
     1921 tatggagcct tgtcgatgct catcgagctt catttgtcgc gggcggccgg accgacgctg
     1981 caggcacaga tcgccgatca actgcgtcgg caaatcctca gcggccgtct gcagtcagat
     2041 tacgagcttc caccgtcgcg tgagcttgcg cgccagtatg gcatttcgcg taacactatc
     2101 atccgtgcct acgaacacct catcagcgaa ggctaccttg cgaccgttaa gggcgtgcgg
     2161 acggtcgtag ctgagtcgat tcctgaggcg tgcctgtcag tcgggaaaga agtagcgccg
     2221 ccgaataacc agtatttcgc cccgcgcgcg cccgtggtct ttgccggcga gaacctcgcc
     2281 gttccggctg cggcgcgccg cacgtcaaaa cttatcgatt tctggccggg ccgcgcgaat
     2341 gttgcacact tcccggcggc cacatggcgg aagctcgctg atgatgtgct cagcgcgcaa
     2401 agccgggagc ttgttgaata tggcgatccg gccgggctgc cagcacttcg cgaagcgatt
     2461 gcgcagcatg tcgcgaattc acgcggggtc cgatgctccg cggaagctgt gatcatcacc
     2521 gctgggacac aggaggccat caatctcgtc gcgcggatgc tggtcgcagg gaacacaggc
     2581 gttgtgattg aggatcccgg ttatggcagc gcggcgcata cgttcagaag ttatggcgcg
     2641 catatgcata cgattcctgt cgatcgcgac gggcttgtga ccgacctctt gccgaaagag
     2701 ggcatcagct tcgcctacgt cacgccgtcg catcaatttc caaccggcgc gacaatgtcg
     2761 gcgccgcgac ggcgcgcgct tctcgattgg gcataccgga ccggcgccta tatcgtggaa
     2821 gacgattacg acagcgatta tagcttcgac ggcccgccgg cgctctcttt ggcgggtgcc
     2881 gacgatgggc aatgcgttat ctacgtcggc actttttcaa agtcgctcgg ggcgggcgtc
     2941 cgaaccggct atctcatcgt tccgccgcag ctcatcgatg tcgcgcgccg ggcgaaagcg
     3001 atgaataatt acggccatcc ctggcttgag caagccatcc tgcaaggctt catcagcaca
     3061 ggcgcttacc agcgccacct tcgcacgatt cgcaaagcca gctcggacac gattggcttc
     3121 ctgatctcgt gtttgcaggc gtctttcgga cagctcgaca tctggggcgt caacggcgga
     3181 atgcacgtca tgtggttgct gccggattgg atgcctcccg cggaggattt caaggggcgc
     3241 cttgagcctc agggcgtcca cgtccacacg ctcgtctccg gcggcgccta ttgccaaacc
     3301 ggtctctatt gtgaccgcgc cattctgctc gggtatgcgg cgctcaccca gcggcagatt
     3361 ggccgcgccg tcgagatcat gcagcgggtg attccgtccg gctatgcctc gtggcgccaa
     3421 gcatagcatc tggcattaaa tttgcttccg gtcagcaatg cttgaatcgc gccggtcgcg
     3481 gcgcgatttt cccttcggcc gcgtccacct agctgggcag cctgatctcc agaagcggtc
     3541 aacgcccatg gcacacaata tcgcggtgga aacccctagc gagccgtctg tgcttcgctt
     3601 gaatgacaaa gactggtttc agcgtctcgg ccaggtcgca aagtcaatcg gctcgcgcga
     3661 gtttcatcat gaactggtcg acctgttcgg cgccagcatc cgacatgatt cctgctggat
     3721 catccggttt tcgagtacgg cgacgcccga ggttcttttc accaacaatg ttgccgacga
     3781 cgtcgtccac acctacagca aaagctattc ggaactcgat ccgttttcgg attattggcg
     3841 gaatttcgag cgcgccggcg tgaccatgct gtcgcaggcc agggcgcaat cgaaacgcac
     3901 aacggcctat agtcaggtgt ttcttccgca ggcccatatc tccgacgaga tgagcatctt
     3961 cctgccgacc gtcgggcgcg attgctttgg tctgttcctc gagcgcgaaa atggccaatt
     4021 ctctaaggcg gatgtcgaac gcgcccgtct tgtttttccc gccttggagg ggtgtcaccg
     4081 ggcgcatatc gcgtgggtct tcaacaacct ccgcgacgtc gaaggcatgg agcttaacgg
     4141 ctttgccaac aagccgatct tgattcagga tcgttttggc gccgaagttt attccaatga
     4201 tgcctggaac gaggcgcgcg tgggcgtggc gctcgacgag ctggttgacg cgtttctcgt
     4261 ccaagatggc ggccatgcgc gctcgttcga cgatttcgtc ctgaaatacg agcctctcga
     4321 tcgagatttc ccgttggcgc cgggcggccg gatgttcgtc ctggagacga atgccgcggc
     4381 ggcgcaaagc gggcaggcct tcgcggatgc gcgggcgtta tttacgccac gcgaatatga
     4441 cgtcttttcg ctgatgatgg agggacgcac gaccggccag atcgcgcagg cgttagaaat
     4501 cagcaaaggc gtcatcaaga actataagct gcgaatttat cgcaaggccg aggtgacttc
     4561 cgagcgggcg ctggtacgca ggttcgcctt agtctgattg gcgctctgcc acaggatgcg
     4621 tgcgcagacg cagcggatca taaaacggca ctttgacgac gcgcgccggg aaggttcctg
     4681 cctcgcttcg cacctgcatt tccattccgt aatccgcaaa tgccggcgcc acatgaacga
     4741 gggcgatcga acgcatcaga tgttcgctgt aggtcgtgct gttgacgatc ccgacgactt
     4801 ccccaccctt gaataatttc gcccctggct cgattgcctt gtcatgatcg atctcaatgc
     4861 ccacctgcgc gatacgcttc tcgccgcgcc gccggatcag cgcctccttg ccccggaacg
     4921 aaggtttgtc cagatcgacc gtccagtcga cacctacctc ccatggagtc gtatcgccct
     4981 ctggcatgtc gaagggaaag aacaaaagcg ccgattcgac gcgaacgata tcgaggcagg
     5041 cccacgacac gggaatggcc ccgaaaggtt ttccggcttc gaggattgaa tcccaaagaa
     5101 aaacagcatc ggcggcgcgg cagaagactt catagcctcg ctcgccggaa tagccgccac
     5161 gcgccagtgt caccttgtgg ccgaagagag tcgtcggcgc gtgttcgaaa tagccgagat
     5221 cggcaagttt taacggcgta tggggcgcca gaatatcaag cgccttcggc ccctgcagcg
     5281 acaagatatg cgtatcgtta tccttgcgga attgaacgtc gcgcccttgt gtgacactcg
     5341 gcagcacgtc ttccagcgcg ccgccgccat gtgagattcg gtagcggtcc ggggcgatgc
     5401 aatagacaag cacatcgtcg atcagcgatc cgttttcatc aacgatattg ctgatcgctg
     5461 attttccggg tgctagcttg gtgatgttcg aggtcagcaa ttcgttcagc gccatgagcg
     5521 cgtccttgcc cgtggcatcg ataagccgca gtgatgtcac atcgaatagg ccggcacgat
     5581 agcgcacggc ttccgtttcg aaatacgggt ccgtcgcgta aagctgcgga ataggcatat
     5641 cgttccacga cgcctcgaac tgcgtcccca gagcgcgatg cctgtcattc aagaccgact
     5701 gacgcgaata gacatgcgta ttcatggcta aatccccgct acgcaccgta gaaatacggt
     5761 gatgccccgg ccgacgccag gcaagtgact tcgggtgcat cgttttcgtg gagaatgtcg
     5821 ctgtcaatga tgccgtttgg catcatggac ttcggccggc tcagaatcta ccgaagatga
     5881 cggtgagcct gtgcgcggcg ccggtgccgg ttcgccatga ttattgtggc gcaagatgtt
     5941 gaaatgccag tcccaacgat ctccagccgg ccacgctatc agccgttcga attgattccc
     6001 gtcgcgggca gccatctcgt cgtggcggag acgcgacagg cgccacgcgc cgacattcca
     6061 aaatcctggg ccgatgcgcc gatcgagttt tggaacgtcg ccgccgagag cgcaatcttc
     6121 gacgccgcaa tcgctcagga tggtatcctt cagcgcggat ttcgctccgc gtcgcatttg
     6181 ctcgatcgcc tgtcgtaccg ccttgagcac gagcatgttg ggctgcatct ttacattatc
     6241 gggacggagc cgtttatctg ggatgtcgtg aagctggcgc gtagtttcgg catggatagc
     6301 gacgaatatc atgtcaccca cgaaggctcc gaaaaacggc gcgtgtattg cgtccattgc
     6361 cgcaccatga cgcaagatgt gacgacaaat atcgcgacct gcacaggctg cggcgcccat
     6421 ttgcaggtgc gcgatcattt ttcgagacgc ctcgcggctt tcatcggcgt gcagatcgat
     6481 gccgaagttc caggtgacat tccgccgatt gaggaggctt tcccgtgaaa gccgcgaagc
     6541 taaacctacg cgtgcgtgct tccgagacga tcgcaccttc attgaggagt ttcaccctcg
     6601 aagcggcaga cgagggtgaa cttcctgcga ggggccccgg cgcgcacctt cggctgatgt
     6661 tgaaagacgg ctcgcaggcc tggcgcaatg cttattcgat cgccgctgca tcgacggatc
     6721 gccgcgaata tcgcattatc gtgcgccgcg tcgcccattc acgcggcggt tcggcgtttc
     6781 tacatgaagc cgtgcggcca ggccagctga tcgaagcgga ttttcccggc aatctcttcc
     6841 ccatcgtgca gatcgcgcgc aaacatctgc tggtcagtgg tggcatcggg atcacgccgt
     6901 ttctctcgta ccttggccat ctcaaggccc tcggcacgcc gtttgaactg caccatttct
     6961 gtcgcgccga ggaagtcgcg attttcgaga atctgctcgc cgaatacgat tcatcgcgca
     7021 tcttcattca tccagaggcg acgcaattcg acctcgtcga gacgttgcgg acgcaacctt
     7081 tggggacgca tctttacgtc tgcgggccgg cggaactcat ggatctggtt atcgcgatcg
     7141 cccgagcgca tggctggccg gcctcgaaat atcattccga atcgttcggc ggcgtgcatg
     7201 cgggcggcgc gcccttcatc gccgtcctcc aacgcgctgg catcgaggtc cgcgtcggcc
     7261 aagatcaaac gctgcttgaa gcgctcgaag cggcgggttt tgaaccatcg tgcctctgtc
     7321 ggggcggcgc ctgcggcgaa tgccgaaccg aagtgatcga gggcactccg gaacatcgtg
     7381 atcattacct tgatgccgac gaacgcgcca ccaaccgcgc aatgatgatc tgcgtctcac
     7441 gcgcggtggg cgatcgcatc gttctcgatc tttgaacagg agcctgcgat gacgattgcc
     7501 ttcaaggtgg ccgagacgtt tcgcgacgac ttcagctatc gcaacagcga agcggccatt
     7561 ctacgtttcc cgtttccgtt cccggaagac aaatacatgt attccgtcaa catcgaaccg
     7621 catgtcgcgg gcggctcttc gccagcctac gaccatgctt ttgatgttga tgagcattac
     7681 atcgcagagt gccgcgaacg cgcgctcgtc ctcgcgcaag atcctggccg gtgcaaagtt
     7741 ctgccgcaca tgatgaccgc gcaatgggat acgctcgaac tcatcatgga gagcctcgcc
     7801 cgcgactacc ccgagcattt ctccctcgcc aagaacggta ccgtctggac ctggaagaac
     7861 cgcccactcg gaatcacgca gaccttcacc ttcggagacg cttcgacgct cccctctgag
     7921 cccttcgaat atattacgcg ccaggcgcag ggtgatttca cactgcagga tcagcgcgac
     7981 aataatcttt tcgtcgatgg cggcatggtg acggcgcagg cggattggtc gctggaattc
     8041 gacgtcggca tggcgttcca cgaatggcac gcgccggtgc cgctggcgca tcagatcggt
     8101 gtcttcgacc gtgcgctgaa atttttgctg cgtctgcagc aggggcagcc ggtgcggcgg
     8161 ctgaattgga cgatgacggt caacccgcgc ctcgacacga gcccagagaa ctacccgctc
     8221 tgggcccccg atcgcctgac ggtgacgcct gagaacatcg ccgaaaaggt taatgtgcgc
     8281 gtcgaattgc agacgctgtt ccggctgccg cgctcgaatg cgattctttt cggcattcgc
     8341 tgctatctgg cgagtatcga agagcttgga cgtgtcgtga aatggcgtaa acgcttgcat
     8401 cgcgtgctgc gcgatctgcc gccggaaatc gaaacctata agggattgac gcgctaccgg
     8461 cagatggcgg tcgattatct cgcaccattt gatgacgggg cgccgctttc gaagggaagt
     8521 caggccgacc agaacgcgct ttgacagcgc cgagtcgctt tcctgcaccg ggccatcttc
     8581 ggtgatatgt gcgcaagatg ggactcatcg agcagtttcg actggacggc ttcaaggctg
     8641 tcaccaaggt ttccccgtcg ccgcatttgg cggcgcgcga tgcctgcgca atcgctgccc
     8701 cggccgacca ttgtcttccc acttttagcg ctgactgccg cgtagttccg caaacgtggc
     8761 ttaattttta agcactttat tttcctgcaa agaaattatg tcttttagcg gcataagtaa
     8821 aatcaataac ttaactaaat acgcgtgttg gcacatatcc tgctgcactg cattatctcg
     8881 atcgcggagc ggacggggtg cgcgcgcaac acggatccga tttccttcta cgcaacgcac
     8941 cgtttgctgc gcacgatcga tcagaccttg aagtacggga agtgcgaaat ggctgaatta
     9001 cgtcgaaaca acctgactct cgtagaggcg gtaggccaat cggtctgcag cctctctccg
     9061 accttcacac ccgcgcttgg cgttgccgtg gttggcggca tggccggcgc aaactcgtgg
     9121 ctggtttacc tgctcgccac gatcagcgtt actatcgtcg gtattaacat cggcaagctt
     9181 gccaaacgca tccccgcggc aggctcgttc ttcctctacg tgtcgcgtac gcttggaccg
     9241 tcttggggct tgctttccgg atgggcgatg ctcgttgcct atctcttcac cgcagtcgcc
     9301 ttgacggttg cgacatcgat cttcttcaaa gatttctttg gcgcgatcgg tttcaaaacg
     9361 ctgccgccgg actggttgat ctatatggcc gtctcggcgc tgatcttcac tctcgcttac
     9421 cgagacgtga agctctcgac gcgattcggc ctcacgcttg aagccctgtc cgtcgcggcg
     9481 atcatcgtcg tctgcttcct catctggaag tcgtttggct tcaaaatcga cgcgccgcag
     9541 gtgcatctgc aaggtgcttc cttcggcacg atcgcgccc
//
LOCUS       DASWOG010000022         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_220501,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000022 DASWOG010000000
VERSION     DASWOG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220501"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VGB93_00425"
     CDS             complement(<1..279)
                     /locus_tag="VGB93_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144626.1"
                     /translation="MLRDAADLRRFMLSRSETTISERSGKPPETDREEVPWHSGASRS
                     LFRGHKNAEAASIFSRQAYESTDKISFRSRVRMAAKALQAQLLDIFGRA"
     gene            complement(323..1015)
                     /locus_tag="VGB93_00430"
     CDS             complement(323..1015)
                     /locus_tag="VGB93_00430"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HEY0144627.1"
                     /translation="MPGKHALLLGLLALLPLSFIAHSSRADTTAPADSSAAPTTEERD
                     FDVTRDGNKIGVETVLIEKTGDTTVVKFKTQISVVVMFIQAYHFAHSAVETWTGGKFV
                     SYKAVTDDNGKHFNVSAVAKDDAVLLDVNGAHSKLPGNMVFASLWSRDFVDQTTLLHP
                     DSAAQLQVKVKDLGEEQIEFHGDQIKAEHYEISGDLDRDIWFEGDKIIRIKLLGSDHS
                     TIMSELMPTKSN"
     gene            complement(1255..2766)
                     /locus_tag="VGB93_00435"
     CDS             complement(1255..2766)
                     /locus_tag="VGB93_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144628.1"
                     /translation="MAEYYPLLAKAIAGLPNSTPETRRAVYERARKALLGQLQNLQPP
                     VPPADLARETESLDAAVARLEAELSGVAPPTPQPPAPQPPVQPSATQPPVTPARPAPR
                     PFTPPPRPASATRPQTPAATSQPPATPVGSRRANGAPALDEPAGEANGRSQPKIEAAP
                     PPTPSFIAPSEPPPLRQPNREARMDVPPGREPGKELRPEPPRMRLSESARPIAPQQDL
                     GDAPVGRRLWIVIGVVVVLVALVAVAAWKLRDRPDQLTAFNRPQNQTADTNSTKITQR
                     AGDADSQPATSAPAASEAQPAPAPANPAPAPAEAVAPKPADSNTALPVAYRAALLVEA
                     PEEPNKVKTYVGTVVWKLENVSNGPGQPLSTAVHADIDVPDDKLKVTVDIQKNTDPSL
                     SASHTITIVFTVGAGSTTGGVKEISAPQLRSEDSPSGEALKGVVVPIMDNSFLVGLTR
                     GDAEAGNIDLLKKLEWIDIPIMLNNGRIAKLTFEKNASGSRAINDAFASWQGQ"
     gene            complement(2874..4172)
                     /locus_tag="VGB93_00440"
     CDS             complement(2874..4172)
                     /locus_tag="VGB93_00440"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0144629.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSLEADVVVRFQGGHNAGHTLV
                     IGNQTYKLALLPSGIVRPGKLSVIGNGVVLDPHHLVAEIAKLESQGVEVSPINLKIAE
                     NVTLILALHRDLDAFRESAITEGVKIGTTKRGIGPAYEDKAGRRALRLMDLTDPDSLD
                     EKIARLLAHHAPLRAGLGMPPVSAADIRREVLSVAPKILPFMAATWDLLETYRRDGRR
                     ILFEGAQGAMLDVDHGTYPFVTSSNTVAANAATGSGLGAKAIGYVLGIAKAYTTRVGG
                     GPFPTELHDEIGELIGDRGHEFGTNTGRRRRCGWFDAVLVRQAVKTSGIDGIALTKLD
                     ILDGLDEIKVCVGYKLDGAKFDHMPAAQAMQQRLEPIYESIPGWKETTAGARSWAQLP
                     AQAIKYVRRIEELIEAPVALLSTSPEREDTILVHNPFQDR"
     gene            4435..>5788
                     /locus_tag="VGB93_00445"
     CDS             4435..>5788
                     /locus_tag="VGB93_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0144630.1"
                     /translation="MGVAALGVVFGDIGTSPLYTLKTVLHVTGGSSQHAVLGVLSLLI
                     WTLIIVTTIKYVSFAMRIDNDGEGGILALMSLLGIKRQHRPYIIVIGLFGAALIYGDG
                     AITPAISVLSALEGLQIAAPHLHNFILPLTVVILIGLFAAQPLGTAKIGRAFGPVMLF
                     WFISIGLLGLAGLVRHPAVLEALSPTYAMSFLFHGGWTSFLVLGAVFLCVTGAEALYA
                     DMGHFGAGPIRLSWYAVVFPSLILNYAGQAALVASGAPTQDNIFYQLCPPQLLMPFII
                     LATLATIIASQSIISGAFSMTKQAINLGWLPRLKITQTSKEGVGQIYVGTVNWILMAV
                     TIGLALAFKKSDNLASAYGIAVSATMLLTTMLLFIAMREIWGWSLAAAGAVAGCFFIV
                     DLSFFLANMTKLAEGGYVPLALAALVYGVMYIWHRGIQAVGWKIAAITTPLKDFCANL
                     Q"
BASE COUNT         1067 a         1747 c         1756 g         1218 t
ORIGIN      
        1 ggcgcgaccg aaaatgtcga gaagttgagc ttgtaaggcc ttggcggcca tccttaccct
       61 gctacggaag ctaattttgt cagtcgattc ataagcctga cggctaaaaa tcgaagcggc
      121 ttccgcgttc ttatggccgc gaaataaaga acgagatgcg ccggaatgcc acgggacttc
      181 ttcccgatcg gtttcgggag gctttcccga tctctcggaa attgtcgttt cgctgcggga
      241 cagcatgaat cgacgcaaat ctgctgcgtc gcgaagcaac caattcaatt atgcgggatg
      301 aagcacggtg tttttcgggt tgtcagttcg acttcgtcgg catcaattcc gacatgattg
      361 ttgaatgatc cgatcctaat aattttatgc ggatgatctt gtcgccctca aaccagatgt
      421 cgcgatcgag gtcgcccgaa atttcataat gctcggcttt gatttgatcg ccgtggaact
      481 cgatttgctc ctcgccgaga tccttgacct tgacttgcaa ttgggccgcg gaatcgggat
      541 gcagcaaggt agtctgatcg acgaaatccc tgctccacag gctcgcaaaa accatattgc
      601 ccggcagttt gctgtgggcg ccgttcacgt cgagtaaaac cgcgtcatcc ttggcaacgg
      661 cgctgacatt gaaatgtttg ccgttgtcat ccgtaaccgc tttataggaa acaaattttc
      721 cgccagtcca agtttccacc gccgaatgag caaagtgata ggcctgaatg aacatgacga
      781 cgacagagat ttgggtcttg aatttgacga ccgtggtgtc accggtcttc tcgatcaaga
      841 cggtttcgac gccaatttta ttgccatccc gagtcacatc gaagtcccgc tcttccgtcg
      901 tcggcgccgc cgaggaatcg gcgggggccg tcgtatccgc gcgactcgaa tgggcgataa
      961 aactcagcgg taggagagcg agcagaccga gaagaagagc gtgctttccg ggcatagatc
     1021 tctctttcgc aatggacgcc atctgattgc cggcaaagtg gttgacgata ctcggcgcgc
     1081 ggaactcgaa tcggctcccg acacgactta gcttggactc tatttcaatg gcaatttgct
     1141 gaatttcggt caatcacgcg ttgtttcaga aaatttctgc attgctctca tgccaggatt
     1201 atggcggccg gccaaattcg tccccaacct cgcgggtcgc ggacggattt tattctactg
     1261 gccctgccat gacgcaaagg cgtcattgat cgcacgtgag ccagaggcat tcttctcgaa
     1321 cgtcagcttg gcgatccggc cattgttcag catgatcgga atgtcgatcc attccagttt
     1381 tttcagaaga tcgatatttc cggcctcggc atcgccgcgg gtcaagccga caaggaagga
     1441 attatccata atcggcacga ccacaccctt caatgcctcg ccgcttggac tgtcctcgct
     1501 gcgcaattgt ggcgcgctga tttccttgac gccgccagtc gtgctgcccg ccccgaccgt
     1561 aaaaacgatc gtgattgtgt gcgaggcaga caggctcgga tcagtattct tctgaatgtc
     1621 gactgtgacc ttcaatttgt cgtcggggac gtcgatgtcg gcgtggacgg ccgtcgacaa
     1681 tggctggccc ggcccattgc tgacgttttc gagcttccag acgacagtcc cgacataggt
     1741 cttcacctta ttcggctctt ccggcgcttc gacgagcagc gcggcacgat aagctaccgg
     1801 cagtgcagtg tttgaatcgg caggtttcgg cgccaccgct tctgccggcg cgggggccgg
     1861 gtttgcgggt gcaggcgcgg gctgagcttc ggacgctgcg ggcgcggatg tcgccggctg
     1921 cgagtccgcg tcaccggcgc gctgggtaat cttggtgctg ttcgtgtccg cagtttgatt
     1981 ttgcggccgg ttgaaggccg tgagctgatc cggccggtcg cgcaacttcc aggccgcgac
     2041 cgccacgagg gcgacgagca cgaccacgac accgatcacg atccaaagcc gccgaccgac
     2101 aggcgcatcg cccaaatcct gctgcggcgc gatcggccgc gccgattcgg aaagccgcat
     2161 ccttggcggc tcgggccgta gctccttgcc aggttcgcgc cccggcggca catccatccg
     2221 ggcctcgcgg ttcggctggc gcaaaggtgg cggctcggac ggcgcaataa acgagggcgt
     2281 cggcggcggg gccgcttcga tttttggctg tgaccggccg tttgcttcgc cggcaggttc
     2341 gtccaacgcc ggagcgccat tcgcgcggcg tgatccaacg ggggttgcag gcggctgcga
     2401 tgtagcggct ggcgtttgcg ggcgcgtcgc cgacgcgggc cttggcgggg gtgtaaacgg
     2461 acgcggcgcg ggacgagccg gggtgacggg tggctgcgtg gcagacggtt ggaccggagg
     2521 ctgaggcgcc ggaggctggg gcgtgggcgg cgcgacgccg ctcagctccg cttcgagccg
     2581 cgcaacggca gcgtcgagcg actcggtttc ccgcgcaaga tcggcgggag gcaccggtgg
     2641 ctgcagattc tggagctgcc ccaggagcgc cttgcgggcg cgctcgtaga cagcccggcg
     2701 cgtttcgggc gtggaattcg gcaggccggc gatggccttg gccagcaagg ggtaatattc
     2761 agccattggc gttcagatcg tctcgctgtg tacggcaaac cgaggcgtaa gcatcatctg
     2821 tggcgcaaat agcaccggcg ggcgcttcaa acacaattcc ctaggcgcaa acactagcgg
     2881 tcctgaaagg gattatgaac cagaattgtg tcttcgcgct ctggacttgt cgaaagcagc
     2941 gccaccggtg cctcgatcag ttcttcgatc cggcggacgt atttgatggc ctgcgcgggc
     3001 aattgtgccc aggagcgcgc gccggccgtc gtttccttcc accccggaat tgattcgtag
     3061 atcggctcga gccgctgctg catcgcctgc gccgccggca tgtgatcgaa tttagcgccg
     3121 tcgagcttat agcccacgca aaccttgatc tcatcgaggc cgtcgagaat gtcgagcttg
     3181 gtcagcgcga tgccgtcgat gccagaggtc ttcaccgcct ggcgcaccag caccgcatcg
     3241 aaccagccgc aacgtcgccg tcgccccgta ttggtgccaa actcatggcc gcgatcgccg
     3301 atcaattcgc cgatctcatc atggagttcg gtcgggaatg gaccgccgcc gacgcgcgtc
     3361 gtgtaagcct tcgcgatgcc caggacatag ccgatcgcct tggccccgag tcccgaacct
     3421 gtcgccgcat tggccgcgac cgtgttcgac gacgtgacga atggataggt gccatgatcg
     3481 acgtcgagca tcgcgccttg cgcgccttca aaaaggatgc gccgaccgtc gcggcgatag
     3541 gtttcgagca gatcccaggt cgcggccatg aaggggagaa tttttggcgc gacggaaagc
     3601 acctccctgc gaatatcggc ggcggagacc ggcggcatgc cgagaccggc gcgaagcggc
     3661 gcatgatgcg cgagcagccg cgcgatcttc tcgtcaagcg aatccgggtc ggtaaggtcc
     3721 atgaggcgca acgcccggcg tccggcctta tcctcatagg caggaccgat gccgcgcttg
     3781 gtcgtgccga ttttcacgcc ttcggtgatt gccgattcgc ggaaggcatc gagatcgcga
     3841 tgcagcgcca ggatcagcgt gacgttttcg gcgatcttca gattgatggg cgaaacttcg
     3901 acgccctgcg attcaagctt ggcgatctcg gcgacgagat gatgcggatc gagcacgacg
     3961 ccgttgccga tgaccgagag tttgccgggg cgcacgatac cagatggcaa aagggcgagt
     4021 ttataagtct ggttgccgat gacgagcgta tgacccgcgt tgtggccacc ctgaaagcgc
     4081 acgacgacat ccgcttcgag cgaaagccaa tcgacgatct tgcccttgcc ctcgtcgccc
     4141 cactgggcac cgaccaccac aacattcgcc attgcgcgcg cggatctccc taaaatctca
     4201 aacaaaaacc ccggcgcgac ctgcaatggc agcaccgggg ctcattgcgc cgaccagata
     4261 cacgagcgcg cctgtcgagt aaaggcggcg gcaggccggt cgcccaagcg cgctgccgca
     4321 ccgcccgtta tgaaaaagcc atgtgtttcc gtgatagcct gatgaaccac cggaaccgcg
     4381 atcatgaatc cagaccagcg tcccgacccg caggccggta aaggccttgc cggcatgggt
     4441 gttgccgcgc tcggcgtcgt cttcggcgac atcggcacga gcccgctcta cacgctgaag
     4501 accgtcctcc atgttaccgg cggctccagc cagcacgccg tgcttggcgt gctgtcgctg
     4561 ctcatctgga cgctgatcat cgtcacgacg atcaaatatg tctccttcgc catgcgcatc
     4621 gataacgacg gtgagggggg gatattggcg ctgatgtcgc tgctgggcat caaacggcag
     4681 catcgtcctt acattatcgt catcggcctg ttcggcgcgg cgctgatcta tggcgacggc
     4741 gcgattacgc cggcgatctc ggttctctct gcgctcgaag gcctgcaaat cgcggcgccg
     4801 catctgcata atttcatcct gccgctgaca gtcgtaattc ttatcgggct gtttgccgcg
     4861 cagccgctcg gcacggcaaa gatcggccgg gctttcggac cggtgatgct gttctggttc
     4921 atctccatcg gcctgttggg gcttgccggc ctggtgcgcc atcccgccgt gctcgaggcc
     4981 ttaagcccaa cctatgcgat gagctttctg tttcatggcg gctggacatc gtttctggtg
     5041 ctcggcgcgg tttttctctg cgtcaccggc gccgaagccc tctatgcaga catgggccat
     5101 ttcggcgccg ggccgatccg cctgtcgtgg tacgctgttg tcttcccgag tctcatcctg
     5161 aattacgccg ggcaggcggc gcttgtggct tcgggcgccc cgacgcagga caatattttc
     5221 taccagctct gcccgccgca attgctgatg ccgttcatca tcctcgccac gctggcaacg
     5281 atcatcgcca gccaatcgat catctccggc gctttttcga tgaccaaaca ggcgatcaac
     5341 ctcggctggt tgccgcggct caagatcact caaacctcaa aggagggggt cggccagatt
     5401 tatgtcggca cggtgaattg gattctgatg gcggtgacga tcggcctcgc gctcgccttc
     5461 aagaaatccg acaatctggc ctcggcctat ggcatcgccg tttcggcgac gatgctgctg
     5521 acgactatgc tgctcttcat cgccatgcgc gaaatctggg gctggagcct cgccgccgcc
     5581 ggtgctgtcg caggctgctt cttcatcgtc gatctgagct tcttcctcgc caatatgacg
     5641 aagctcgccg aaggcggcta tgtcccgctc gccctcgcgg cgctcgtcta tggcgtgatg
     5701 tatatctggc accggggcat acaggcggtc ggctggaaga tcgcggccat aacaacgcca
     5761 ctcaaagatt tttgcgccaa tctgcaaa
//
LOCUS       DASWOG010000023         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_238809,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000023 DASWOG010000000
VERSION     DASWOG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238809"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1893)
                     /locus_tag="VGB93_00450"
     CDS             complement(106..1893)
                     /locus_tag="VGB93_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0144631.1"
                     /translation="MTESPEHYKSVLLFLGTAAIVAPVFRRLKISPVLGFLAAGFCLG
                     PYGLGALVTRGTLPGWVSHVTFDNLDEIAPVAEFGVVFLLFMIGLELSFERLLHLRRL
                     VFGFGAAQVFVSALALGGIALALHHSLGSAVILGLALAMSSTAIVLPVLADRKRLGTS
                     AGRTIFSVLLFQDLMVAPLLFLVTILGAHHDKFDAVLLYTVLPACIGIAVVALAGRLV
                     LRPLFHFVVAAGSPEFFVAACLLVVVGTAVISAVSGLSMGLGALIAGLLLAETEYRRQ
                     IEVTIEPFQGLLLGLFFLSVGASLDLSLIFSAPVRTLGLAGGFIAVKAVTVYFAGRLF
                     RLSGTVASETALMLAPGGEFALVVIASALASGALGAQIGADALVAVTLSMFVIPPMAG
                     LAALLPRPKPEADAELEKSPAPLEEGAIGKAIIVGYGRVGQVVGEMLRAHAIDFLAVE
                     TSAGLVKQFRSLGTDIYWGNASQREFLVRCGIAKARALIVTIGAVEGSEDIVAAARHL
                     NKDLTIVARARDASHARELYELGVTDAVPETVEASLQLSEAALVDMGVPMGLVIASIH
                     EKRDEYRKALQLAGATKMREFGARRHRSR"
     gene            complement(2089..2556)
                     /locus_tag="VGB93_00455"
     CDS             complement(2089..2556)
                     /locus_tag="VGB93_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY0144632.1"
                     /translation="MRIPIAPFIGVLLAFVAGYVDTATFLRVSELFSAHITGNFVVFA
                     IALVHGVKGVDWLKLIALPVFFLGVPLATFVYDRSRQKIRTGRASESARRVGVGITEL
                     LPHSAAYDAGLAMLLVLAMAIQNAAHQSSGTKPWADIRRDNDQRRQTFCRFMA"
BASE COUNT          632 a          841 c          800 g          391 t
ORIGIN      
        1 gatgacgtca taacgcgcgg ctagttcgtg cagaccagtg acgaaacctg gcgggctttc
       61 agaaggcatt acgaaacgtc tcacatccag tgggcaaaca aaacatcatc gactgcgatg
      121 gcgccgggct ccgaactcgc gcattttcgt ggcgccggca agttgcagcg ccttacgata
      181 ttcgtcacgc ttttcgtgga tcgaggcgat cacgagcccc atcggaacac ccatgtcgac
      241 cagagcggcc tccgacaatt gcaagctcgc ctcgaccgtc tcgggcaccg cgtcggtgac
      301 gccaagctcg taaagctcgc gcgcatggct cgcatcgcgg gcgcgggcga cgatggtgag
      361 gtccttgttg aggtgccggg cggcggcaac gatgtcttcc gatccctcca ccgcgccgat
      421 cgtcacaatc agtgcgcgcg ccttggcgat accgcagcga accagaaact cgcgctgcga
      481 cgcgttaccc caatagatat cggtgcccag cgagcggaat tgcttcacca gacccgcgct
      541 ggtctcgacg gcaagaaaat cgatcgcatg cgcccgcaac atctcgccca ccacctggcc
      601 aacgcggcca tagccaacga tgatggcctt gcctatcgcg ccttcttcca gcggcgctgg
      661 cgatttttcg agttcggcat cggcctcggg ctttggccgc ggcagcaagg ccgccaagcc
      721 ggccatcggc ggaatgacga acatcgacaa agtgacagcg accagggcgt ccgcaccgat
      781 ctgcgcgcca agcgccccgc tcgccaaagc gctggcaatc acgaccaaag caaattcacc
      841 gccaggcgcc agcatcaatg ccgtttctga agcgaccgtc ccgctgaggc ggaacagccg
      901 tccggcaaaa tagaccgtga ccgctttgac cgcgatgaaa ccgcccgcaa gtccgagcgt
      961 ccggaccgga gcggaaaaga tcagcgacaa atcgaggctg gcgccgaccg agagaaaaaa
     1021 caggccaagc aacaggccct gaaacggctc gatcgtcact tcgatctgac gccggtattc
     1081 cgtctcggcg agaagcaggc ccgcgatcaa cgcgccgagt cccatcgaaa gtccgctcac
     1141 ggccgaaatc accgccgtgc cgacgacaac caggagacag gcggctacaa aaaattccgg
     1201 cgagccggcg gcgactacga aatgaaacag tggccgcagg acgaggcgcc ccgcgagcgc
     1261 gaccacggcg atgccaatgc aggccggaag caccgtgtag agcagaaccg catcgaactt
     1321 gtcgtgatgg gcaccaagga tcgtcaccag gaacaacaaa ggcgcgacca tcagatcctg
     1381 aaacagcagg acggagaaaa tcgtccgccc tgccgatgtg cccagccgtt tgcgatcggc
     1441 aagcaccggc aggacaatcg ccgtcgatga cattgcgagc gcaaggccca ggatcacggc
     1501 cgaccccagc gaatgatgca gagccaatgc gatgccgccg agcgccagtg cggagacgaa
     1561 aacctgcgcg gcgccaaatc cgaaaaccag ccggcgtaga tgcagcaacc gctcgaaact
     1621 cagttctaac ccgatcatga acaagagaaa gacgacgcca aattctgcaa ccggcgcgat
     1681 ctcatcgaga ttgtcaaacg tcacatgcga gacccagccc ggcagcgtgc cgcgtgtgac
     1741 gagtgcgccc aagccgtaag ggcctaggca aaagccggcc gcgagaaaac cgaggacggg
     1801 gctgatcttg aggcgccgga agaccggcgc gacgatcgcg gccgtaccga gaaacagcag
     1861 aacgctttta taatgttcgg gcgactccgt cattgagtcc tgtttaatcc ctatggctca
     1921 tgaaacaatg cgacaaacgc aacgacgagc cctggcagca agatagccgc caacccaaaa
     1981 ctattcgccg caaaggcgga tacggcgcag ccgagcgcga agcaaacaag gcgcgcgcca
     2041 ataaccttgg ctttgagcgg tggcgccgcg gacggtggcg gactcgtttt acgccataaa
     2101 gcgacaaaaa gtctggcggc gttggtcgtt atcacggcgg atgtcggccc aaggcttggt
     2161 tccacttgat tgatgagcgg cattctgtat ggccatcgcc agcacgagca gcatggcgag
     2221 cccggcatcg taggcggcgg aatgcggcaa gagctcagtg atgccgaccc ccacccgccg
     2281 cgccgattcg ctggcgcgcc ccgtccggat tttttgccgc gatcggtcgt agacgaatgt
     2341 cgccaagggc acgccgagga agaacactgg cagcgcgatg agcttcagcc aatcgacccc
     2401 cttcacgcca tgcaccagcg caatggcgaa aacgacaaaa ttaccggtga tgtgggccga
     2461 aaaaagctcg gagacccgca ggaatgtcgc ggtgtcgaca tagccggcga cgaaggcgag
     2521 cagaacgcca atgaacgggg caatcgggat tcgcataggc atcgcactcc gggtcacgat
     2581 gcgggatggc ccagcgggcc ggacgcgtca aggttaccgg ggagacgccc tttcttttcg
     2641 ggacgttagc gaccatcttt gcgg
//
LOCUS       DASWOG010000024         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_257165,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000024 DASWOG010000000
VERSION     DASWOG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257165"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGB93_00460"
     CDS             complement(<1..359)
                     /locus_tag="VGB93_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0144633.1"
                     /translation="MASTLGHADRRAPFRSYCMGLILPGERKSVEPIAARVEPRRVQG
                     AHQSLHHFVANADWSQEDLLRRVRTFVLPAIERSGRISAWIVDDTGFPKKGVHSVGVA
                     RQYCGQLGKQDNCQIAVS"
     gene            569..1406
                     /locus_tag="VGB93_00465"
                     /pseudo
     CDS             569..1406
                     /locus_tag="VGB93_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_167331742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          329 a          470 c          460 g          302 t
ORIGIN      
        1 gagaccgcga tctgacaatt gtcctgcttg ccaagctgac cgcaatattg ccgagcgacg
       61 ccgacagaat gaacgccctt cttgggaaat cccgtgtcgt cgacaatcca ggcgcttatc
      121 ctgccgctcc tttcgatggc aggaagaaca aaggttcgaa cgcgtcgcag cagatcctcc
      181 tgcgaccagt cggcgttggc gacaaaatgg tgcagcgact gatgtgcgcc ttggacccgg
      241 cgcggctcga cgcgggccgc tatcggctcg acgctttttc gctcgccggg aagaatgagc
      301 cccatacagt aggagcgaaa cggcgcacgc cgatccgcgt gccccagggt cgacgccatg
      361 tgctccacat aagacgcaaa tcgagcctcg cttccctcgc tatccgcatt tcgatccacc
      421 ggcccctcca aatcaggcgt gaatcgaaat catcccccaa atagtttatg acacagtaag
      481 actaagtgtc tgtccgggaa gatattgaat cgtctgagtc atttgtgatt caagggaggc
      541 ggcctgattc tttggggagc cgccgccgat gtggacgaag gaaaaccgcg ctcgctacga
      601 ccgcagccgc ctgcgctatc cgtccgacct gaccgacgag gagtgggcgt tgatcgagcc
      661 gcatattcca ccggccaggc gcggcggcaa caagcgaacg gtgaacttgc gcgaggtcgt
      721 caacggcttg atgtacatgc ctcaagcgcc ggttgccagt ggcgggcgat cccgaaggac
      781 ctgccgccgc gctcgacggt gtatgattat tttgatctgt ggacctggga cggcacgctc
      841 gatcggatcc atgccgctct ctatgtcgaa tgccgcgaaa gagcagagcg cgaggcgtct
      901 ccgacggcgg ccatcatcga cagccagagc gtgaaaagcg ctgaaaaagg ggggctcacg
      961 acccgcatgg ctacgatgcg ggcaagaaga tcaaaggcaa gaagcggcat attctcgtcg
     1021 acacgcaggg cctcctgatg cacgccatcg tgcatggcgc cgatattcag gaccgcgacg
     1081 gcggcgtgct cctaatggcg acattgttcg gcctctatcc cttccttctg aagctctacg
     1141 ccgatggcgg atatcaaggg gcgcagttcc agaatgcgct cagggccatt ctcaagcgcg
     1201 tgaacgtcga gatcgttaaa cgctccgatc aagcccagcg cttcgtcgtc ttgcccaaac
     1261 gatggatcgt cgagcgaacc ttcgcctggc tcggacgatg ccgaaggctc gccaaggatt
     1321 gggagtgcct caatcgcaag gcgcttgcct tcctcaagct cgcttctatc cgcctcatga
     1381 cccgaaaatt atgtaattca agatgatgtt tccggataga ctctgagagt ctgtccggac
     1441 acattatgat ggattgaacc gctccggtcc gaggtgcgcg cccgcgcggt gcgaatggtc
     1501 ttggatcacg aaggcgagca tgcctcgcgc ttggcggcgg tctcgtcgat cgccgcccag
     1561 a
//
LOCUS       DASWOG010000025         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147171,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000025 DASWOG010000000
VERSION     DASWOG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147171"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /gene="malQ"
                     /locus_tag="VGB93_00470"
     CDS             complement(<1..1475)
                     /gene="malQ"
                     /locus_tag="VGB93_00470"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0144634.1"
                     /translation="MSNETLHQLARRAGLLVDWIDASDKPQRVSDESLRRILDALDFP
                     CATETEAARSLQESSANTARPGLVTGEVNALVLLPGVRENRARLMLENGTQEEARLDK
                     SASGGASLRVSRPGYHKLEFSNRIIKLAVAPKRCLTLEDVGHGAKLWGLAVQLYGLRR
                     PGDAGIGDTSALTALGKSAAARGADAIAISPTHALFAGRPDKFSPYAPSNRLLLNPLL
                     AGVDALLDGRSLDVDATFDNSTSLIDWPAATARKTALLRVPFESFFQRDDDDELVRDF
                     RMFRQTSGDVLESHARFEAIHAHIIAHDPSNSGWRTWPQEFSSPNKPAVAAFAASHTR
                     EVTFHAFLQWLADRAFGKAQTDMREAGMRIGLIADLAVGMESGGSYVWAHQHETLSPL
                     TVGAPPDLFNQQGQNWGLTTFSPRALIEHDFEPFLATLRSAMRHAGGIRIDHILGLKR
                     LWVIPEGVPSSEGAYLNYPFDDLLRLIRLESMRQRAIVIGE"
     gene            complement(1472..3241)
                     /gene="treZ"
                     /locus_tag="VGB93_00475"
     CDS             complement(1472..3241)
                     /gene="treZ"
                     /locus_tag="VGB93_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY0144635.1"
                     /translation="MTQSFSRDLPFGARLLPSDRTRFRLFAPAQQNVQIEIEGQDTGS
                     MRRSDEGWFEAEAPCGAGAHYLYVLDDGSRVPDPASRGQVPDVHGWSVVVDPAAYLWQ
                     IPEWRGRAWQETIIYELHCGLYGGFRGVAAALPRLADLGVTAVELMPINDFSGTRNWG
                     YDGVLPFAPDGSYGSADDLKALVDAAHAHGLMIFLDVVYNHFGPDGNYLSRYAPTTFR
                     DDFHTPWGAGIDFQRPEVRRLFTENALYWLLEYRFDGLRLDAVHAISEPGWLDEMAAE
                     IRASVEDGRKVHLILEHEGNVASHLRGDFDAQWNDDLHNAMHVLLTGETEGYYQDYAE
                     DTVLKLARGLAEGFIYQGEPSPHHGGAARGTPSADLPPTSFVFFLQNHDQIGNRAFGE
                     RLTVLCPPEALEAAIALQILTPQIPLLFMGEEDGSQTPFFFFSDYEGELADAVREGRR
                     KEFARFKAFTDPAMREKIPDPNAASTFEQSRPQPNSEHGAARFAFYRRLLTLRQSLIV
                     PRLAGARAVRANALGKAAIRARWRLGDGSNLTLLTNLDAEAVSAAAPPGAVIFESRPC
                     VSHAVAARTLPGRTTIAYLEAQG"
     gene            complement(3238..>3975)
                     /locus_tag="VGB93_00480"
     CDS             complement(3238..>3975)
                     /locus_tag="VGB93_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY0144636.1"
                     /translation="DGFTLMDTVSYAERHNETNGEDNRDGSSENYSANWGTEGPTDDQ
                     NINETRARLCRAMLATVLFSHGTPMLLGGDEFGRTQQGNNNAYCQDDEISWTNWKLAE
                     APDGQSMIDFVSRLLALRREHPVLRCRHFLHGKEELAKGIRDISWFNYHGDPIPGDLW
                     NNPDKRAIVLRRAEKTPDGRVVALTILLNPIAEKREFQLPSPYVPSSVLVNSGDPALA
                     PYNLNEDKITLMPQSVVLLRGYHEGLP"
BASE COUNT          859 a         1210 c         1134 g          772 t
ORIGIN      
        1 tcctcgccaa tgacgatcgc gcgttggcgc atcgattcca gacggataag gcgcagcaga
       61 tcgtcaaacg gataattgag ataggcccct tcgctggagg gaacaccttc agggatgacc
      121 cataatcgct ttaggccgag aatatgatcg atgcggatcc cgcccgcatg gcgcatcgcc
      181 gagcgcaacg tcgcgagaaa tggctcgaaa tcatgttcta tcaaggcgcg cggcgaaaat
      241 gtcgtcagtc cccaattctg gccctgctga ttgaagagat ccggcggtgc gccgacggtc
      301 agcggcgata aggtttcgtg ctgatgcgcc cagacatagc tgccgccgct ctccatgccg
      361 acggcgaggt ccgcgatgag gccgatccgc atgccggcct cgcgcatgtc cgtctgcgct
      421 ttgccgaacg cccggtctgc gagccactgg agaaacgcgt gaaaggtgac ttcacgcgtg
      481 tggctggcgg cgaaagcggc tacggcaggc ttattaggtg acgagaattc ctgcggccag
      541 gtacgccatc cactattgct gggatcgtga gcaataatat gcgcgtggat tgcttcgaaa
      601 cgcgcatggc tttcgagaac gtcaccagac gtttggcgaa acatccgaaa atcgcgaacc
      661 aattcgtcgt catcatccct ttgaaaaaag ctttcaaatg gcacgcgcag aagcgccgtt
      721 ttacgcgcag tcgcggccgg ccaatcgatc aacgacgtgg aattatcgaa tgtggcatcc
      781 acatcgagag agcgtccgtc aagcagcgca tccacgcccg caagcaacgg atttagcaaa
      841 agccggttcg acggcgcata ggggctgaat ttgtctggac gccccgcgaa tagtgcatgc
      901 gtcggactga tcgcaatcgc atccgcgcca cgcgccgcgg cggactttcc aagcgccgtg
      961 agcgcactcg tatcaccgat cccggcgtcg ccgggtcggc gaagaccgta aagctgtaca
     1021 gccaggcccc agagcttcgc gccgtgcccg acatcttcca gcgtcaagca acgcttgggc
     1081 gccacggcta gtttgattat acgattgctg aactccaatt tgtgatagcc gggccgcgac
     1141 acccgcagcg acgcgccgcc gctcgcgctc ttgtccagcc gggcttcctc ttgcgttccg
     1201 ttttccagca taagccgcgc ccggttttcg cgaacgcccg gaagcaagac caaagcatta
     1261 acttcaccag tcaccaagcc cggacgtgca gtattggcgg acgattcctg caggcttcgc
     1321 gcggcttctg tttcggtcgc gcacgggaaa tcaagcgcat cgagaattct tcgcagcgac
     1381 tcgtccgaaa cgcgctgcgg cttgtcgctg gcgtcgatcc aatccacgag aagaccggcg
     1441 cgtcgtgcaa gctggtggag cgtttcgttg ctcacccttg cgcctccaga taagcgattg
     1501 tcgtccgccc cggcaaagtc cgggccgcaa cggcatgcga tacgcatggg cggctttcaa
     1561 agatcaccgc acccggcggc gctgcagccg agacagcctc cgcgtccaaa ttcgtgagaa
     1621 gtgtcagatt ggagccatcg ccgagccgcc aacgcgcgcg gatggcggct ttgccgaggg
     1681 cgttggcgcg aacagcgcgc gcgcccgcaa gccgcggcac aatcaaggat tggcgtaggg
     1741 tcagcagccg tcggtaaaac gcaaaacggg ccgcgccgtg ttcggaatta ggttgcggcc
     1801 tggattgctc gaacgtcgat gccgcgttcg gatcgggaat tttttcgcgc atcgccggat
     1861 cggtgaacgc cttgaaacgc gcgaactctt ttcgccgacc ctcgcgcacc gcgtccgcga
     1921 gttcgccctc gtaatcgctg aaaaagaaaa atggcgtttg gctgccatcc tcttccccca
     1981 tgaagagaag cgggatttgc ggtgtaagga tttgcaaagc gatcgccgct tccaacgctt
     2041 ccggcgggca aagcaccgtc aggcgctcac caaaagcgcg gttgccgatc tgatcatggt
     2101 tttgcagaaa aaatacgaaa gatgtcggcg gcagatccgc actcggcgtg ccgcgcgcgg
     2161 cgccgccgtg gtggggcgac ggttcaccct gataaatgaa accttcggcc aggccccttg
     2221 ccaatttgag cactgtatcc tcggcatagt cctggtaata gccctccgtc tcgccggtca
     2281 acagcacatg catggcgttg tgcaaatcgt cgttccattg cgcatcgaaa tcaccgcgca
     2341 aatggctggc gacattcccc tcgtgttcga ggatgagatg caccttgcgt ccgtcttcca
     2401 cgctggcccg aatttccgcc gccatctcgt cgagccaacc cggctcgctg atcgcgtgga
     2461 cggcatcgag acgcagaccg tcgaaacgat attccaacag ccagtaaagt gcgttctcgg
     2521 taaagagacg ccgtacttcc gggcgctgga aatcgatgcc tgccccccac ggcgtatgaa
     2581 agtcatcacg aaatgtcgtc ggcgcataac ggctaagata atttccatcc gggccgaaat
     2641 gattatagac aacgtcgaga aaaatcatca gcccatgcgc gtgcgcggca tcgactaggg
     2701 ccttgagatc gtccgccgaa ccgtaagaac catctggcgc aaatgggagc acaccgtcat
     2761 aaccccaatt gcgcgtgccg gaaaaatcgt tgatcggcat caactcgacg gccgtcacac
     2821 cgagatcggc caggcgcggc agcgccgccg caacaccgcg aaagcccccg taaagcccgc
     2881 aatgaagctc atagatgata gtttcttgcc aagccctgcc gcgccattcc ggtatttgcc
     2941 aaagataagc tgcgggatcg acaacaacgc tccacccatg cacatcgggc acctgtccgc
     3001 gcgaagcggg gtccggaacg cggctgccat cgtccaagac ataaagataa tgcgcgccgg
     3061 cgccgcacgg tgcctccgcc tcgaaccaac cctcgtcaga ccggcgcata gatccggtat
     3121 cctgaccctc aatttcgatt tggacgttct gctgcgccgg cgcaaatagc cgaaaacgcg
     3181 ttcggtccga aggcaaaagg cgcgcgccaa atggcaaatc gcgcgagaag ctctgcgtca
     3241 tggcagacct tcatgatagc cacgcagaag cacgacgctc tgtggcatca acgtgatttt
     3301 gtcttcgttg aggttatacg gggctaacgc cggatcgccg gaattgacga ggacgcttga
     3361 aggtacatac ggcgatggaa gctgaaattc gcgtttttcg gcgatcggat tgagcagaat
     3421 ggtcaaggcg acgacgcggc catccggcgt cttctccgcg cggcgcaaaa cgatcgcgcg
     3481 tttgtcagga ttattccaaa ggtcaccggg gatgggatct ccgtgataat tgaaccatga
     3541 aatatcccga atgccctttg caagttcttc cttgccatga agaaaatggc ggcatcgcaa
     3601 gacaggatgc tcgcgtcgca gtgctagcaa tcgcgaaaca aaatcgatca tcgattgacc
     3661 gtctggggcc tcggccagct tccaattcgt ccacgaaatt tcgtcgtcct gacaataggc
     3721 gttgttattg ccttgctgcg tccgtccaaa ttcatccccc ccgagaagca tcggcgtccc
     3781 gtgcgaaaat aataccgtcg ccaacatggc gcggcacaga cgtgcacgag tctcgttgat
     3841 attttgatcg tcggtcggcc cttcggtgcc ccaattcgcc gaatagttct cggaacttcc
     3901 gtcgcgatta tcttcgccat tggtctcgtt gtggcgctcc gcgtagctga cggtgtccat
     3961 cagcgtgaag ccatc
//
LOCUS       DASWOG010000026         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147173,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000026 DASWOG010000000
VERSION     DASWOG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147173"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..248)
                     /locus_tag="VGB93_00485"
     CDS             complement(3..248)
                     /locus_tag="VGB93_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEY0144637.1"
                     /translation="MPVSIELAIPMFITIWFVVLFAILPIGIHSQRESGTYVEGTDPG
                     APVNPQLLKKALLTTLASLIVFGVLMIVLRLCGWGVA"
     gene            complement(327..725)
                     /locus_tag="VGB93_00490"
     CDS             complement(327..725)
                     /locus_tag="VGB93_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0144638.1"
                     /translation="MTSELRQPPAARQWPRELRLGEAGKVLKVSFESGRGFDLSAEYL
                     RVMSPSAEVQGHLPDERKTLGGKRNVAVTEIVPIGSYAVRLSFDDSHSTGIYTWDYLY
                     ELGARRTEKWAQYLAELAAKDLSRDRPGEK"
     gene            785..1843
                     /gene="moaA"
                     /locus_tag="VGB93_00495"
     CDS             785..1843
                     /gene="moaA"
                     /locus_tag="VGB93_00495"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0144639.1"
                     /translation="MNQSCTDLKPAPLTQLLQTQARPLVDPFGRAISYLRVSVTDRCD
                     FRCVYCMSEDMSFLPKGDLLTLEELDRLCSVFAARGTRKLRLTGGEPLVRRNIMQLFH
                     GLGRHLDSGALDELTLTTNGSRLAFFAPELAACGVRRINVSLDSRDPETFKAITRRGD
                     LAQVLRGIDAAQSAGIDVKINMVALKGVNEGEIVPMISWAHARGMDLTLIEVMPMGAI
                     EPDRLDQYLPLSDVRAEIAKHFTLRDLAYRTGGPARYVEVAETGGRLGFITPLTHNFC
                     ESCNRVRLTCTGTLYMCLGQNDAADLRAPLRASASNDALNAAIEEAITRKPKGHDFMI
                     DRTHTAPAVPRHMSVTGG"
     gene            complement(1847..>2329)
                     /locus_tag="VGB93_00500"
     CDS             complement(1847..>2329)
                     /locus_tag="VGB93_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY0144640.1"
                     /translation="LRDVAWGELDCLVVDMPPGTGDAQLTLAQNVALAGAVIVSTPQD
                     LALFDARRGIAMFNKVNVPVLGIVENMSYFLCPHCGERSDIFAHGGARHEAEKLGVPF
                     LGEVPLALEIRAHSDDGAPIVVAAPESAYAAAYRDIAARVQVGLVGAQVRQPPKIVIE
                     "
BASE COUNT          479 a          735 c          655 g          460 t
ORIGIN      
        1 gcttatgcca cgccccaacc gcaaagccgc agcacgatca tcaggactcc aaagacgatc
       61 agcgaggcca atgtcgtcaa aagcgccttt tttaacaatt gcggattgac cggcgcgccg
      121 ggatcggtgc cttcgacata ggtgccggac tcccgctgcg aatgaattcc gatgggcaga
      181 atcgcaaaca gcacgacaaa ccagatcgtg atgaacatcg gtatagcaag ctcaatagaa
      241 accggcatct cgaaccttct gcaatggggc attcgcgtca gtataagcta tatcgctcaa
      301 tgtatagctt gcgtattcca aagatattat ttctcgccgg gacggtcgcg gctgaggtcc
      361 ttggcggcga gttcagcaag atattgcgcc catttttcgg tccgcctggc gccgagctcg
      421 tagaggtaat cccaggtgta gatgccagtg ctgtgcgaat catcaaagct gagcctgacc
      481 gcatagctgc caatcggcac gatctccgtg acggcgacat tccgcttgcc gcccagggtc
      541 ttacgttcgt ccggcagatg tccctgcacc tcggcgctgg ggctcatgac gcgcagatat
      601 tccgccgaca gatcgaaacc gcggccgctc tcgaacgaaa ctttgagtac cttcccggct
      661 tcccccaggc gaagctcacg cggccattgc cgtgccgctg gaggttggcg aagctctgac
      721 gtcattgcac caaactttct ttggtcctat tcaagctttt actttcgtgg actatatcaa
      781 acgcatgaac cagtcctgca ctgatttaaa accggcgccc ctgacgcagt tgcttcaaac
      841 gcaagcgcgg ccgctggtcg atccgttcgg tcgagcgatc tcctatctgc gcgtttcggt
      901 gacagatcgg tgcgacttcc gttgcgtcta ttgcatgtcg gaagatatga gctttctgcc
      961 gaaaggcgat ttgctgacgc ttgaggaact ggaccggctc tgcagcgtct tcgccgcccg
     1021 tggcacgcgc aagctgcggc tgaccggtgg cgaaccgctc gtgcgccgca atatcatgca
     1081 actttttcat ggcctcggtc gccatctcga ttcgggcgcg ctcgatgaat tgacgctgac
     1141 gaccaatggc tcgcgcctcg cctttttcgc gccggagctt gcggcctgcg gcgtgcgacg
     1201 catcaatgtc tcgctcgaca gccgcgatcc cgagacattc aaggcgatca cccgccgcgg
     1261 cgatcttgcg caagtgctgc gcggcatcga tgcggcacag agcgccggca tcgacgtcaa
     1321 gatcaacatg gttgcgctca agggcgtcaa cgaaggagag atcgttccga tgatctcctg
     1381 ggcgcatgcg cggggcatgg atctcaccct catcgaagtg atgccgatgg gcgctatcga
     1441 accggatcgg ctcgatcaat atctgccttt gagcgatgtg cgcgccgaga tcgccaagca
     1501 tttcaccttg cgtgatcttg cctatcgcac cggcgggccg gcccgctatg tcgaagtggc
     1561 cgaaaccggc ggccggctcg gtttcatcac gccgttgaca cataatttct gcgagagctg
     1621 caaccgtgtc cgcctcacct gcaccggcac gctttatatg tgccttggcc agaacgacgc
     1681 ggcggatttg cgcgcgccgc tgcgcgcatc ggcgagcaac gatgcattga acgccgcaat
     1741 tgaagaagcc attacccgca agccaaaggg tcatgatttc atgatcgacc ggacccatac
     1801 cgccccggct gtgccgcggc acatgagcgt gacgggcggc taagctctat tcgatcacga
     1861 tcttcggcgg ctggcgcact tgcgcgccga caagccctac ctgcacccgc gcggcaatgt
     1921 cacgataggc cgccgcataa gcactctccg gcgccgcgac gacgatcggc gcgccatcat
     1981 cggaatgggc gcgaatttcg agcgccagcg gcacttcgcc aagaaacggc acgcccagct
     2041 tttccgcctc atgccgcgcg ccaccatgcg cgaaaatatc cgaacgctcg ccacaatgcg
     2101 gacagagaaa ataactcata ttctcgacaa tgccgagcac cggcacattg accttgttga
     2161 acatggcgat gccgcgccgc gcatcgaaca acgccagatc ctgcggggtc gagacgataa
     2221 cggcaccggc cagcgcgacg ttctgcgcca gcgtcaattg cgcatcgccg gtgcctggcg
     2281 gcatatcgac gacgaggcaa tccaattcgc cccaggcaac atcgcgcag
//
LOCUS       DASWOG010000027         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_330491,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000027 DASWOG010000000
VERSION     DASWOG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330491"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGB93_00505"
     CDS             complement(<1..327)
                     /locus_tag="VGB93_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEY0144641.1"
                     /translation="MPEFDAPDLRSAHVIVVANEKGGTGKSTLSIHISVALLKAGFRV
                     STIDLDTRQRTLTRFFENRASWAANAPWDVELPRHHALDRGNSENVRDNETWEFGAFA
                     AAISEVE"
     gene            complement(548..1810)
                     /locus_tag="VGB93_00510"
     CDS             complement(548..1810)
                     /locus_tag="VGB93_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0144642.1"
                     /translation="MRVSRRCGGCLISLPDNTGSPHVLIAGAGVGGLTAAIALARRGW
                     RITLIERGDGNENIGAGLQLSPNASGILRDLGVLPGLAAAGLAPDAIHIRRARDGATL
                     ARLPLADAERRWGAPYLNVHRADLVRILRETALADPNVSFHPQTILAGFEQTANGVRA
                     VGLRGPIRIGFEADCLIGADGVRSFVRARSAALRGKLDDMPTQTRYAAWRTLIAAAGV
                     AEDMRLPASNLWLGQGAHVVHYPLRAGKMINIVAVVDTREMLDEKADLWSQKGDPAWI
                     AARLAHWAPPIRDLAAKAAEWRVWPLIERETPAAWSHGRVALLGDAAHAMLPFLAQGA
                     AQAIEDAAALAAALDPGGDIIGALAAYSAKRAPRAARVQSESRRQARIYHLGLPASLA
                     RDMALRSLGSRLSRRYDWLYGAKNPAIS"
     gene            complement(1777..2028)
                     /locus_tag="VGB93_00515"
     CDS             complement(1777..2028)
                     /locus_tag="VGB93_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY0144643.1"
                     /translation="MADHGTPHFHNQPGVARVRIGAKKFMCMGALPPFDHPHIFIDMG
                     DADDAICPYCSTHYVHDASLAPGACEPAECAFPDAAAAA"
     gene            2136..2903
                     /locus_tag="VGB93_00520"
     CDS             2136..2903
                     /locus_tag="VGB93_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0144644.1"
                     /translation="MQEFSSHGVRIAYLDALPAEVEKGRPVLLIHGFASNHAVNWVFP
                     QWLKTLNAAGRRAIAFDNRGHGRSEKLYRPEDYELSLMAADARNLLDHLGVESADVMG
                     YSLGARIATAFALAHPERANAFVFGGLGGKLLETPGLGDVIARALEAPSLADVEEGLG
                     KTFRTFAEATKSDLRALAACARGARRQFTEADLAAVAAPVLVSVGSKDDVAGDPKTLA
                     SHLPSSEALVIPDRDHNRSVGDATFKRAALDFLERWA"
     gene            2992..3828
                     /gene="cysE"
                     /locus_tag="VGB93_00525"
     CDS             2992..3828
                     /gene="cysE"
                     /locus_tag="VGB93_00525"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY0144645.1"
                     /translation="MSSAKSSLASASRERTDSIFVRIRREAEELLHRDLSIAPWVTTI
                     ILEQPSFEAAVIYRVASRLGNDTIAVEQIRQTYSDIAARDPAIGVAFRADLLAFLDRD
                     PCCERLIEPLLYFKGFHALATHRLAHALWTEGRYDFALYLQSRSSEVFQTDIHPAAKI
                     GKGIFLDHATGLVVGSTAVVEDNVSLLQDVTLGGTGKEAGDRHPKIRHGVLIGAGAKV
                     LGNIEVGHCARIATGSVVLRPVPPKTTVAGVPAQIVGDAGCPEPALSMDQILAAKAAA
                     RG"
     gene            3919..4314
                     /locus_tag="VGB93_00530"
     CDS             3919..4314
                     /locus_tag="VGB93_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEY0144646.1"
                     /translation="MDKVELQKLQGFLRQAFGNQSLKVVPAKRNSDDAEVHLGERTVA
                     SIAVDDEDGDRSFALEMKIPVERPVLQDYLRRLFENDKLRIVPRPRKNDSVELNSGDD
                     YLGVISQDDPKGSSFTLQVAILDFDLEDF"
     gene            4358..4903
                     /locus_tag="VGB93_00535"
     CDS             4358..4903
                     /locus_tag="VGB93_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0144647.1"
                     /translation="MPLHALDGVFPQLAKDAWAAPDAQIIGDVRIGAEASIWFHAILR
                     GDNEKITIGARTNIEDGCILHTDIGYPLEIGEGCTIGHRVILHGCLIGANSLIGMGAT
                     LLNGVNVGRNCIVGANSLLTEGKAYPDNSLIIGAPARVVRELRDSEIAQLRESAAVYV
                     ANARRYKRGLALAGDGVRAKA"
BASE COUNT          933 a         1511 c         1581 g          964 t
ORIGIN      
        1 ttcgacttcc gaaatcgccg cggcgaacgc gccgaattcc caagtctcgt tatcgcggac
       61 attttcggaa ttgccgcggt cgagggcgtg gtggcgcggc agttcgacat cccacggcgc
      121 attggcagcc caggaggcac gattttcaaa aaaacgggtg agggtgcgtt gacgcgtgtc
      181 gagatcaatc gtcgacacgc gaaatccggc tttcaacaag gccacgctga tgtggatcga
      241 caatgtcgat ttgcccgtgc cgcctttttc attggcgacg acgatgacgt gcgcgctgcg
      301 caagtcgggc gcgtcgaact caggcatcgc ggctcgcgca ttcatgtctc aagtctcaat
      361 ccgcgtgaat gcaccagcat gttcaaagtt caccgcgatt ggtgcggtcg ccgattggaa
      421 gcgtggccga gaatgccacg ggcataccaa ttgttcccgt tcgtcccgga aaaccgttag
      481 gccttccgct tagcttgggc gatgcaagct ctacgctcac ggcgaccgag tcaagcgcac
      541 aactagctca cgaaatagcg ggatttttcg cgccataaag ccaatcataa cggcggctta
      601 ggcgcgatcc gaggctccgc agcgccatat cccgcgcaag cgaggccggc aggccgagat
      661 ggtagatgcg tgcctgacgg cgtgactcgc tctgcacccg ggcggcgcgg ggcgcgcgtt
      721 tggcactata ggcggcgagg gcgccgataa tatcgccacc gggatcaagg gcggccgcca
      781 gggccgcggc atcctcgatg gcctgggcgg cgccttgcgc gagaaatggc agcatcgcgt
      841 gcgccgcgtc gccgagcaaa gcaacgcgac cgtggctcca agccgctggc gtctcgcgct
      901 cgatcagcgg ccagacgcgc cattctgcgg cttttgcggc gagatcgcga atgggcggcg
      961 cccaatgcgc caaccgcgcc gcgatccagg cgggatcgcc cttttgcgac cagagatcgg
     1021 ctttctcgtc gagcatctcg cgggtatcga cgaccgcgac gatattgatc atttttccgg
     1081 cgcgcagggg gtagtgcaca acgtgcgcgc cctgaccgag ccagagattg ctggctggta
     1141 ggcgcatatc ttccgcgacg ccagcggcgg caatgagggt gcgccacgcg gcataacgcg
     1201 tctgcgtcgg catgtcgtcg agtttgcccc ggagcgcggc gctgcgcgca cggacgaagg
     1261 agcgcacgcc gtcggcgccg atcaggcaat cggcctcgaa gccgatacgt atgggtccgc
     1321 gcagaccgac ggcgcgcacg ccatttgccg tctgctcgaa gccggctagg atggtctgtg
     1381 gatgaaagct gacgttcggg tcagcaagcg ccgtctcgcg cagaatccgg acgagatcgg
     1441 cgcgatgtac gttgaggtag ggcgctcccc agcgccgttc ggcatctgca agcggcaaac
     1501 gcgccagcgt cgcgccgtcg cgagcgcgac gaatgtgaat cgcgtctggc gcgaggcccg
     1561 cggcggcgag ccccggcaat acgccaagat cgcgcagaat gccgctcgca ttcggcgaaa
     1621 gctgcaagcc ggctccgata ttttcgttgc cgtcgccccg ttcaatgagc gtgatccgcc
     1681 agccgcgccg cgcaagcgcg attgcggccg ttaggccgcc gactccggcc ccggcaatta
     1741 aaacgtgcgg cgatccggta ttgtcaggca acgaaatcag gcagccgccg cagcgtcggg
     1801 aaacgcgcat tccgccggtt cgcaggcgcc aggcgccagg ctagcgtcgt gcacgtaatg
     1861 cgtcgagcaa taggggcaga tggcatcgtc cgcgtcgccc atgtcgatga aaatatgcgg
     1921 gtgatcaaac ggcggcagag cgcccatgca catgaatttc ttggcgccaa tccgaacacg
     1981 cgcgacgccg ggctgattgt ggaaatgcgg ggtgccgtgg tctgccatcg gaaaatccca
     2041 ttcaccgggc tggtgggttc cgctttattc gaatagagcg gcaaggtcta caggacgcgg
     2101 cggacgatcc gcccccattc atgattttgg ctcagatgca ggaattttcg tcgcacggcg
     2161 tgcggatcgc ctatctggac gcgctgccgg cggaagtgga gaagggccgt cccgtcctcc
     2221 tgatccacgg ttttgcgtcg aaccacgccg tcaactgggt gtttccgcaa tggctgaaga
     2281 ccctgaacgc tgccgggcgt cgcgccatcg ccttcgacaa tcgcggccac ggccgcagcg
     2341 aaaagctcta ccggcctgaa gattatgagc tttcgctgat ggcggcggat gcgcgcaatt
     2401 tactcgatca cctcggcgtc gagtcggccg atgtgatggg ctattcgctc ggtgcccgta
     2461 tcgccacggc cttcgcgctc gcccatccgg aacgcgcgaa cgcctttgtt tttggtggcc
     2521 tcggcggaaa actcttggaa acgccagggc ttggcgacgt catcgcccgg gctctcgaag
     2581 ccccgtcgct ggccgatgtc gaagaaggac tcggcaaaac cttccggacc ttcgctgagg
     2641 caacaaaaag cgacctgcgg gcgctcgccg cctgcgcccg tggcgcccgg cgtcaattta
     2701 ccgaggcaga ccttgccgcg gtcgctgcgc ctgtcctggt ctccgtcggc agcaaggatg
     2761 acgtggctgg cgacccgaag acgctagcct cacatctgcc atccagcgag gcgctcgtca
     2821 ttcccgaccg tgatcataat cgcagcgtcg gcgacgccac gttcaaaagg gcagcgctgg
     2881 actttctcga acgctgggcc tagccgaggg ccggcggcgg ttgtgcgccg cgacgttgct
     2941 gcaatcgcct acaaaaacat gctagaagag ataaattttt cggaggttga ggtgagttcg
     3001 gcaaaatcgt ctttggccag cgccagtcgc gagcgcacgg attcgatctt tgtgcgcatt
     3061 cgccgcgaag ccgaagagct tctccaccgc gacctgagca ttgcgccctg ggtgacaaca
     3121 atcatcctcg aacagccgag ttttgaggcg gcggtcatct atcgcgtcgc gagccggctc
     3181 ggcaacgaca cgatcgcggt ggaacaaatc cgccagacct attcggatat cgccgcccgc
     3241 gatccggcga tcggcgtcgc tttccgcgcc gaccttcttg cgtttctcga ccgcgatccg
     3301 tgctgcgagc ggctgatcga accgctactt tatttcaaag gatttcatgc gctggcaacc
     3361 caccggcttg cgcatgcgct ctggacggaa gggcgttacg atttcgcgct ttatttgcag
     3421 agccgatcgt ccgaagtctt tcaaaccgat atccatcccg cagccaagat cggcaaaggc
     3481 atctttctcg atcatgcgac cgggctcgtg gttggctcga ccgcggttgt tgaagacaat
     3541 gtctcactgc tgcaggatgt cacgctcggt ggaaccggca aggaagcggg cgaccgtcat
     3601 ccgaagattc gtcacggtgt cctcattggc gccggcgcca aggttctcgg taatatcgag
     3661 gtgggccatt gcgcgcggat tgcgacgggc tccgttgtcc tgcgtcccgt gccacccaag
     3721 acgacagttg ccggcgtccc ggcgcaaatc gttggcgatg ccggctgccc ggagccggcg
     3781 ctttcgatgg atcagatcct tgcggcaaag gcggccgccc gcggctgagc cggggttgcg
     3841 atcaatttcg atttctggca attggcacag gggtcgcgtc gcggccttgt gggaagagcg
     3901 gcttgggaga gctggcgttt ggacaaggtc gaactgcaga aattgcaggg ctttttgcga
     3961 caagcctttg gcaatcagag cctcaaagtc gttcctgcca agcgcaattc cgacgatgcc
     4021 gaagtgcatc tcggtgagcg taccgttgcg tcgattgccg tcgatgacga ggacggggat
     4081 cgttccttcg cgctggagat gaaaatccct gtcgaacggc cggttttgca agattatttg
     4141 cggcgtctgt tcgagaacga caagctgcgc atcgtgccgc ggccgcgcaa aaacgattcg
     4201 gtcgaactta attccggcga cgattatctt ggcgtcatct cgcaagacga tccgaagggc
     4261 agcagcttta cgctgcaggt cgccatcctg gatttcgatc tcgaagattt ttgatccgct
     4321 acgtcgtcgt catcgcggct gaatgagtga ggaagctatg cctctccatg cgttggacgg
     4381 tgtgtttccg caattggcga aagacgcttg ggccgccccg gacgcgcaaa tcatcggcga
     4441 tgtcagaatt ggcgccgagg cttcgatctg gttccacgcc atcctgcgcg gcgacaatga
     4501 gaagatcacg atcggcgcgc gcaccaacat tgaggatggc tgcatccttc acacggatat
     4561 cggctatccg ctcgaaatcg gcgagggctg caccataggc caccgcgtca ttctgcacgg
     4621 ctgtctgatc ggcgccaatt cgctgatcgg catgggcgcg acgcttttga atggggttaa
     4681 tgtcggccgg aattgtatcg tgggtgccaa ttcactcctg accgaaggaa aagcttatcc
     4741 cgataattcg ctgattatcg gcgcgccggc gcgggtcgtt cgcgaactaa gggactcgga
     4801 gatcgcgcaa ttgcgcgaga gcgcggcggt ctatgtcgca aacgcacgcc gctacaagcg
     4861 cggtttagcg ctcgcgggcg atggtgttcg cgctaaagct taatgttttc gtcgaatctt
     4921 tcgggcggga tcacctgatc gaagcggacg gccagcccac cttcgaaggc gcggacaatc
     4981 tcgccgttc
//
LOCUS       DASWOG010000028         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_92347,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000028 DASWOG010000000
VERSION     DASWOG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92347"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1017
                     /locus_tag="VGB93_00540"
     CDS             172..1017
                     /locus_tag="VGB93_00540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0144648.1"
                     /translation="MQGKTVVITGATSGIGLVAAERLAGMGARLVLIARDQYRGDVAM
                     TKLRAANPNAAHAIHYADLSTIAEMKRVAATIAAAEAKIDVLINNAGALFNPRRETAD
                     GLEMTFATNHMAYFLVTHGLLDRLKATPGSRIVSTASDAHKGQTLDFDDLQARRSYSA
                     FKVYGRSKLANILFTRELAKKLAGTGVTANCLHPGFVATRFGDNSQGLLKVAIGLAKS
                     LIAISPEKGAQTIVYLASSPEVAQASGGYYYKNKLATPTAQAQDDASAKRLWQDTEKL
                     AGVSW"
     gene            complement(1022..1357)
                     /locus_tag="VGB93_00545"
     CDS             complement(1022..1357)
                     /locus_tag="VGB93_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0144649.1"
                     /translation="MFSPIHPGEQLREEFMKRRGISCERLAEEIGVETDMVTLIVTER
                     EAVSSEMALRLSRYFGTTARFWLDLQRDYDLDRTTHALGDRLDFEVKPTLDGINFGVI
                     ERRARPRLG"
BASE COUNT          306 a          509 c          495 g          297 t
ORIGIN      
        1 cgcggctacg attggataca atccgcgttc ctcaggtaaa tgcgcccgtg gctggcggct
       61 gtcaagggag tcgcccgacg atccccgaga tcgccagggc gggcttgtgg tcgctttccc
      121 gggccctaag tctttgttgc accgcgttct gcggctgggg ggaaagcgat catgcagggc
      181 aagaccgtcg tcatcaccgg cgcgacttca ggaatcgggt tggtggcggc cgagcggctg
      241 gccggcatgg gcgcacggct ggtgttgatc gcgcgcgacc aataccgtgg cgacgtggcg
      301 atgacgaaac tccgtgccgc caatcccaac gcggcccatg cgatccacta cgccgacctg
      361 tccacgatcg ccgagatgaa gcgcgtggcg gcaaccatcg ccgccgccga ggccaagatc
      421 gatgtgctga tcaacaatgc cggcgcgctc ttcaatcccc gccgcgagac ggccgatggg
      481 ttggaaatga ctttcgccac gaatcatatg gcgtatttcc tggtgaccca cgggctgctc
      541 gaccgcctga aggcgacacc cgggtcacgt atcgtcagca cggcatcgga cgcccataag
      601 ggacagacac tcgatttcga cgacctgcaa gcgcggcgca gctacagcgc gttcaaagtc
      661 tatggccgct cgaagctggc caatatcctc ttcacgcggg aactcgcgaa gaagcttgcc
      721 ggcaccggcg tgaccgccaa ttgcttgcac ccgggtttcg tcgcgacgcg tttcggtgac
      781 aacagccagg gccttctcaa agtcgcgatc gggctggcca aaagcctgat tgccatctcg
      841 ccggaaaagg gggcacagac gatcgtctat cttgcctcat cgcccgaggt cgcgcaggcc
      901 agtggcggtt actactacaa gaacaagctc gccaccccga ccgcgcaggc gcaagacgat
      961 gcttccgcca agcggctgtg gcaggacact gagaaattgg ccggggtcag ctggtagcgc
     1021 gtcagcccag gcgggggcgt gcgcggcgct caatcacgcc gaagttgatg ccgtccaatg
     1081 tcggcttcac ttcgaagtcg agccggtcgc ccaacgcatg agtcgtgcgg tccaggtcat
     1141 agtcgcgctg caggtcgagc cagaagcgcg ccgtggtgcc gaaatagcgc gacaggcgca
     1201 gcgccatctc gctcgaaacc gcttcccgct ccgtaacgat caaggtcacc atatccgtct
     1261 ccaccccaat ctcttcggcc agccgttcgc acgaaatgcc gcgccgcttc atgaattctt
     1321 cgcggagctg ttcgccgggg tggatgggag aaaacatggg tgaggtccct ttactgatac
     1381 ggtgcttact cgatcgcgac ggtacatttc cctcggcatc gtccggcttc ggtcctcgcg
     1441 tggcccgtgc cggaatttgg ggcgatcttt ctcacgccga tgccatcccg agcaagcgaa
     1501 ggttcggttg caagatcgcc aggaagtctc ggaatgctcc cctgaatcaa ctcgttgtgt
     1561 agcgaagagt cacaatgccg ttgcgcgccc ttctagcgat cgaatgt
//
LOCUS       DASWOG010000029         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_275569,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000029 DASWOG010000000
VERSION     DASWOG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275569"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGB93_00550"
     CDS             <1..333
                     /locus_tag="VGB93_00550"
                     /inference="COORDINATES: protein motif:HMM:NF020350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY0144650.1"
                     /translation="GTIRLRAIKPEYVSNDSQRKEAVVMIKHPNNTGMQMDQETHLYI
                     PARYIETLRVTQGDDLIFAVTGGISISEDPNFRFDYVSNGASGMSVTAVDTKGEKFAK
                     AGALEPAM"
     gene            complement(349..732)
                     /locus_tag="VGB93_00555"
     CDS             complement(349..732)
                     /locus_tag="VGB93_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144651.1"
                     /translation="MSIKCAGRPDIQCVSLADVTDRVCEALDDVGESVHRLQHLIAPL
                     VLEAAARNPAHLRELQDFDHISQKLKNLADFLGALALILPEDWQVDPTIATKVLTLAD
                     LSTHLSFAKKDEDAPHEPGDFEMFS"
     gene            complement(744..1307)
                     /locus_tag="VGB93_00560"
     CDS             complement(744..1307)
                     /locus_tag="VGB93_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590475.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEY0144652.1"
                     /translation="MTLHNAAHRAEHTEERQQKVHIIQGQYYVSDQPNVVLLTLLGSC
                     VAACIHDPVARVGGMNHFLLPGADRDTNTRDAERYGVHLMELLVNGLLQRGARKDRLQ
                     AKLFGGARTLEKLSDIGRQNSIFAERFLKTEGIAYLGGSCGGESGRRLQFWPVSGRAR
                     QAMIAGQEVPIIVPPPAPRVDGDVEFF"
     gene            complement(1304..1693)
                     /locus_tag="VGB93_00565"
     CDS             complement(1304..1693)
                     /locus_tag="VGB93_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0144653.1"
                     /translation="MPFASAIRAMIVDDQLTSRALIRDGLQQLGIQDIEQAGDGEQGL
                     KALLQKPAHLVISDLNMPKLDGLQFLQAVRAHPTTQKAAFIMLTGRGDRALIERAIKL
                     GVNNYLVKPFTIPVLKGAIEAVFGKLK"
     gene            complement(1672..>1999)
                     /locus_tag="VGB93_00570"
     CDS             complement(1672..>1999)
                     /locus_tag="VGB93_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEY0144654.1"
                     /translation="NAGPVNGHRPSVDVMFNSVCIAAKSKAVGVILTGMGRDGADGLL
                     AMRRAGAMTIGQDRATSLVYGMPRVAFEVDAVEKQLPLEKIGNEILRLTNRDEEGVKA
                     CRSRQP"
BASE COUNT          422 a          586 c          577 g          414 t
ORIGIN      
        1 ggcacgatcc gtctgcgtgc catcaaaccg gaatatgtga gcaacgattc acagcgtaag
       61 gaagctgtgg tgatgatcaa gcaccccaac aacaccggga tgcagatgga tcaggagact
      121 cacctctata ttccggcgcg ctatatcgaa accttgcgcg tgacccaggg cgacgatctg
      181 atcttcgccg tgacgggtgg catctcgatt tcggaagacc cgaatttccg ctttgactat
      241 gtctcgaacg gcgcgagcgg catgtcggtc acggccgtcg atacgaaggg cgagaaattc
      301 gccaaggccg gcgcgctcga gcccgcgatg taaaacgttg cgctgcggct aactgaacat
      361 ctcaaaatcg ccgggctcgt gcggcgcgtc ttcgtccttt ttggcgaagc tcagatgcgt
      421 ggagagatcg gcaagcgtca agactttcgt ggcgatggtc ggatcgacct gccagtcttc
      481 cggtaaaatg agcgccagcg cgccaaggaa atcggccagg tttttgagtt tctggctgat
      541 atgatcgaag tcttgtaatt cgcgtaaatg cgcgggattg cgtgcagccg cttccaagac
      601 cagcggcgcg atcagatgct gaagacggtg tacggattca ccgacatcgt cgagagcttc
      661 gcacacgcgg tccgttacat cggcgagcga gacgcattga atgtccggcc gacccgcgca
      721 tttaatagac atcgcatcga tcctcaaaaa aattcaacat cgccatcgac acgcggcgcc
      781 ggcggcggca caatgatcgg cacttcctga ccggcgatca tggcctgtcg cgcccggccg
      841 gacaccggcc agaattgcag ccgccgtccg ctctcgccgc cacaactgcc gccgaggtaa
      901 gctattccct cggtcttgag aaatcgttcg gcgaaaatcg aattttgacg gccaatgtcg
      961 gataatttct caagcgtccg cgcgccgccg aaaagttttg cctgaagccg atccttgcgg
     1021 gcgccgcgct gcaataatcc gttaaccaaa agctccatca gatgtacgcc gtagcgttca
     1081 gcgtcgcgcg tgttggtatc tcggtccgcg cccggcaaca gaaaatgatt catgccgccg
     1141 acccgcgcga ccgggtcatg aatgcaggcg gcgacacaag agccaagcaa cgtcaggagc
     1201 acaacattcg gctggtcgga gacgtaatac tgaccttgaa tgatgtgtac tttttgctgg
     1261 cgctcttcgg tgtgttcagc tctgtgtgcg gcattatgga gcgtcatttg agctttccga
     1321 agacggcttc gatcgcgccc ttcaggaccg gaatggtgaa cggcttgacc agataattat
     1381 tgaccccgag tttgatcgca cgctcgatca aagcgcggtc gccgcggccc gtcagcatga
     1441 tgaaggcggc cttttgcgtc gtcggatggg cgcgcaccgc ttgcaaaaac tgcaagccgt
     1501 cgagctttgg catgttgaga tcggagatga cgagatgcgc cggcttttgc agcagcgctt
     1561 tcagtccctg ctcgccatca ccagcttgct caatatcctg gatcccgagc tgctgcaggc
     1621 catcgcgaat cagagcgcga ctggtgagtt ggtcgtcgac gatcatcgcc cttatggctg
     1681 acgcgaacgg catgctttta ctccttcctc gtcccgattc gtgagacgca gaatttcatt
     1741 gccgatcttt tcgagcggca attgcttttc cacagcatcg acctcgaagg cgacgcgggg
     1801 cattccgtaa acaaggctcg tcgcgcgatc ctgcccgatc gtcatggcgc ccgcgcggcg
     1861 catagcgaga agcccatcgg cgccgtcgcg gcccatgccg gtgagaatca caccaacagc
     1921 cttcgatttg gcagcgatac acaccgagtt gaacatgaca tcgacagacg gccgatggcc
     1981 gttgaccggt ccggcgttg
//
LOCUS       DASWOG010000030         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_183961,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000030 DASWOG010000000
VERSION     DASWOG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183961"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGB93_00575"
     CDS             complement(<1..550)
                     /locus_tag="VGB93_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0144655.1"
                     /translation="MSVQLSSKPLSLNYQAIAEDFAELAVAAGAAIMLFYNNDSNARQ
                     KGDTSPVCDADLAGEAVILAGLAARMPELPVVSEEAAAAGAQSTDDDTFILVDPVDGT
                     REFLAGKGEFTVNIGLIVRGEPRVGVVYAPALEQMWLAGDHAVTFPVAPGAKLPPPTE
                     RTRFTVRKQDREKRVARTSWSHT"
     gene            886..1557
                     /locus_tag="VGB93_00580"
     CDS             886..1557
                     /locus_tag="VGB93_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEY0144656.1"
                     /translation="MTEFALGSLDLAALLCSRVCHDVISPVGAIVNGLEMLDEEKDPE
                     MRGFALDLVKKSAHTASARLQFCRLAFGAAGSAGAAIDTGDAEKVTRGLLEDDRTKLS
                     WNLPRILMAKDKVKLILNLCLIAAAAIPRGGVITVNVDTSEPTPHIDVEANGTNARLA
                     SHVPQLLACAPENNQVDAHGIQAFYTGLIARAAQLSLNVTAEPEKVRIEAHPATTDAL
                     ADTLA"
     gene            1759..>2418
                     /locus_tag="VGB93_00585"
     CDS             1759..>2418
                     /locus_tag="VGB93_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0144657.1"
                     /translation="MDDLLSDFLIETSEQIDAAGEQLVLFERDPSDREAIIRIFRLFH
                     TIKGTCGFLSLSRLELLTHVTESLISKLRDGAPATSETVSLILTAVDRVKTILANLTD
                     VGKEPDGDDRDLIAALESDASATSRAPENAPLTAQPKTDAGANKNPSPRTMPSSRADS
                     IRVSVGALERIMILVSELVLTRNQLLEITRHQEDAIIKTQLQRLSGLKSDLQDAVMSA
                     RM"
BASE COUNT          510 a          726 c          678 g          504 t
ORIGIN      
        1 ccgtatgaga ccagcttgtc cgggcgacgc gtttctcgcg gtcctgtttg cgaactgtga
       61 atctggtccg ctctgtcggt ggcggcaatt tagcgccagg cgcgaccgga aacgtgacgg
      121 catgatcgcc cgcgagccac atctgttcga gagccggcgc atagacgacg ccgacccttg
      181 gttctccacg cacgatgagc ccgatattga ccgtgaattc gcccttcccg gccaggaatt
      241 cacgcgtgcc gtcgacaggg tcgaccagga tgaacgtgtc atcgtcggtc gattgggcgc
      301 ccgccgccgc ggcctcttcc gagactaccg gcaactcggg catccgcgcc gcgagccctg
      361 cgagaatgac cgcctcgcct gcaagatcgg cgtcgcaaac cgggctcgtg tcgcctttct
      421 gccgcgcatt actgtcattg ttatagaaca gcatgatcgc agcgccagcg gcaaccgcga
      481 gctcggcgaa atcctcggcg atcgcctgat agttcaggct caacggcttt gagcttaatt
      541 ggactgacat tggatcctgc agaccccgcg actcacacgc cgcttatgag gtgaagatat
      601 tggcttcccg gcggctaatg aaaggcctat cggcgcaatg gttgcaaacg tccttgccgg
      661 gtgtaacaaa aagccgtcgc tgcgcaagtc gattcgccgc gggtttctgt cgcaatgtcg
      721 agatttatct tgcacttgcg aagaatctac gccgctatac cgcgctgcaa cagaacagat
      781 tcgatcaggc ggtggccatt ctaaagttta gcccgccggc tcctaaacgg ggtctctcgc
      841 agagcttgga ctgaaggtcc ctcgctgtac tccaaaggac ggaacatgac agaattcgcc
      901 ctcggctctc tcgatcttgc ggctctctta tgctcgcgcg tgtgccatga cgtcatcagc
      961 ccggtcgggg ccatcgtcaa cggactcgaa atgctcgatg aggaaaagga tcccgagatg
     1021 cgcggcttcg cgctcgatct cgtgaagaag agcgcccaca cggcttccgc gcggctgcaa
     1081 ttctgccggc tggcttttgg tgcagcgggt tcagcggggg ctgccatcga taccggcgat
     1141 gccgaaaagg tcacgcgcgg tcttctggaa gatgatcgca ccaaattgag ttggaacttg
     1201 ccgcgcattc tgatggcgaa ggacaaggtg aaattgatct tgaatctttg cctgatcgcc
     1261 gcagcggcaa tcccgcgcgg aggcgtgatc acggtgaatg tcgatacgag cgaacccacg
     1321 ccgcatatcg atgtcgaagc caatggaacc aatgcccgcc ttgcgagcca tgttccgcag
     1381 ttgctcgctt gcgcgccgga aaataaccag gtcgacgcgc atggcattca ggcgttctat
     1441 acggggctga tcgcacgcgc ggcgcaatta tcgctaaacg taacggccga acccgaaaaa
     1501 gttcgcatcg aggctcaccc agccacaacc gatgctttag cagatacatt agcataacta
     1561 tcccgcgcat aagccgtcgt gggctgtttc cttcgtacgg atagaaacgg catcgaaccg
     1621 gctaatattt gcgcgaaatt attgcgtctt ttattcgtaa gcggctttga atggccgcgt
     1681 tttttcaacg ctctttaacc cattcagcgc aataattctt cgccggggct tgtccttggc
     1741 tggagacttg gcgcagctat ggatgacctg cttagcgatt tccttatcga aaccagcgag
     1801 caaatcgacg ccgccggcga acagcttgtt ctgtttgagc gcgatccctc cgatcgcgaa
     1861 gccatcatcc gcatcttccg tctgttccac acgatcaaag gaacttgcgg ttttctcagc
     1921 ctctcgcggc tcgaactgct cacacacgtt acagaaagtc tgatcagtaa attgcgcgac
     1981 ggcgcgccgg cgacgtcaga gaccgtctcg ctgattttga ctgcggtcga tcgtgtgaaa
     2041 accatcctcg ccaacctcac ggacgtcggc aaagagcctg acggcgacga tcgggactta
     2101 atcgcggcgt tggaatccga cgcgagcgcg acgtctcgcg cacctgaaaa tgcaccgctc
     2161 acagcccagc ccaagacgga cgcgggcgcc aacaaaaatc cgtcgccgcg aaccatgcca
     2221 tcgagccgcg ccgatagcat ccgcgtgtcg gtcggcgctt tggagaggat catgatcctc
     2281 gtctccgaac tggtgttgac acgcaaccag cttctggaga tcacccggca tcaggaagat
     2341 gcaatcatca agacgcagct gcagcgtctc tctggtctta aaagcgatct gcaagatgcg
     2401 gtcatgagcg cacgcatg
//
LOCUS       DASWOG010000031         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_129034,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000031 DASWOG010000000
VERSION     DASWOG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129034"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VGB93_00590"
     CDS             <1..742
                     /locus_tag="VGB93_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0144658.1"
                     /translation="LAPVGEFALVVIASSFASRALGAQIGADALVAVTLSMFVIPPMA
                     GLAALLPRPKPEADADLEKLPAPLEEGASAKAIIVGYGRVGQVVGDMLRAHAIDFLAI
                     ETNAGLVKQFRSRGTDIYWGNATQPEFLARCGIANARALIVTIGAVEGSEGIVAAARQ
                     LNKDLTIVARARDASHARELYELGVTDAVPETVEASLQLSEAALVDMGVPMGLVIASI
                     HDKRDEYRKALQPAGATKMREFRARRHR"
     gene            778..1680
                     /locus_tag="VGB93_00595"
     CDS             778..1680
                     /locus_tag="VGB93_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEY0144659.1"
                     /translation="MPSESPPPHFVTGLRELAANYDVILCDVWGVIHNGVVQYEKATD
                     ALRHFRERGGSVVLITNAPRARARVESFLDNLHVPREAYDGIVTSGDVTISLIRERAG
                     LPLAYIGPPEDMSLFDEAQSALSDRLRFVGLDAAAYAVCIGLEHAEQETPEDYDARLR
                     MLRAHNAEFICANPDIVVEMGDKLVYCAGALAESYEALGGRVVQAGKPHAEIYRRALA
                     LAAGVRGQPIDPARVLAIGDSAHTDIKGGEAQGFDTLFITSGIHRAELHAQTGAGPLD
                     AAALHQFLDHLGFAPTGTAAELLW"
     gene            complement(1686..2081)
                     /locus_tag="VGB93_00600"
     CDS             complement(1686..2081)
                     /locus_tag="VGB93_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0144660.1"
                     /translation="MIVEPSLPILIVDDYKTMVRIIRNLLGQIGFTDIDEASSGAEAL
                     EKMHDKSYGLIISDWNMEPMTGYQLLQKVRENKDVAEVPFIMVTAESKTDNVIAARKA
                     GVSHYIVKPFNATTLKAKIDSVFTMAAVA"
BASE COUNT          386 a          627 c          684 g          471 t
ORIGIN      
        1 gctggcgccc gttggtgaat tcgctttggt cgtgattgcg agctcttttg cgagccgggc
       61 ccttggcgcg cagatcggcg cggacgccct ggtcgccgtc actttgtcga tgttcgtcat
      121 tccgccgatg gccggtttgg cagcgctgct gccgaggccg aagcccgaag ccgatgccga
      181 cctcgaaaaa ttgccggcgc cgctggaaga gggcgcgtcg gccaaggcga tcatcgttgg
      241 gtatggccgt gtcggccagg tggtcggcga catgcttcgc gcgcatgcga tcgattttct
      301 tgccattgag accaacgcgg gtctggtgaa gcaattccgc tcgcggggca ccgacattta
      361 ttggggcaat gccacgcagc ccgagtttct ggctcgctgc ggtatcgcca atgcacgggc
      421 gctgattgtg acaattggcg cggtggaggg atcggaaggc atcgttgccg ccgcccggca
      481 gctcaacaag gacctaacca ttgtcgcccg cgcccgcgat gcgagtcatg cgcgcgagct
      541 ttatgagctc ggcgttaccg atgcggtgcc cgagacggtc gaagcgagtt tacaattgtc
      601 agaggccgcg ctggtcgaca tgggcgttcc gatggggctc gtgatcgcct cgatccatga
      661 caagcgcgat gagtatcgca aggcgctgca gccggccggc gcgacgaaaa tgcgcgagtt
      721 ccgtgcgcgg cgccatcgat gatgtttcat ttgcaaccgg atgagagacg tttcgcaatg
      781 ccttctgaaa gcccgccgcc gcatttcgtt actggtctgc gcgaacttgc cgcgaattac
      841 gacgtcatcc tctgcgatgt ctggggcgta attcacaacg gcgtcgtcca atacgagaag
      901 gcgacggatg ccttgcggca ttttcgcgaa aggggcggct ctgtcgttct catcaccaac
      961 gccccgcgtg cacgggcgcg ggtcgagtct ttcctcgaca atttgcatgt gccgcgcgag
     1021 gcctacgacg gcatcgtgac gtctggcgat gtcacgattt cgctgattcg cgaacgcgca
     1081 ggtctgccgc tggcttacat cgggccgccg gaggatatgt cgctctttga cgaggcccaa
     1141 tccgcgctaa gcgatcggct gcgtttcgtc ggcctcgacg ccgcggcgta tgccgtctgc
     1201 atcggcctcg aacatgccga acaggaaacg ccggaagatt atgacgcgcg attgcgaatg
     1261 ttgcgtgcgc ataacgccga gttcatctgt gccaatcccg atatcgtcgt cgagatgggc
     1321 gacaagctcg tctattgcgc gggcgccttg gcggagagtt atgaggcatt aggcgggcgt
     1381 gtcgtccagg ctggcaagcc gcatgcggag atttatcggc gtgccctcgc gcttgctgcc
     1441 ggcgtgcgcg ggcagccgat cgatccagca cgcgtgctgg ccatcggcga ttcggcgcac
     1501 accgacatca agggcggcga agcgcagggc ttcgacacgc tttttatcac gagcggcatc
     1561 caccgcgccg aattgcacgc ccaaacagga gccggcccgc tggacgcggc ggctttgcat
     1621 caatttctag atcatctcgg ctttgcaccc acgggcaccg ctgcggaatt gctctggtga
     1681 gcagttcagg cgacggcagc catcgtgaaa accgagtcga ttttggcttt gagtgtcgtg
     1741 gcgttgaacg gcttgacgat gtagtggctt acccccgctt tgcgcgcagc gattacgttg
     1801 tcggtttttg actccgccgt caccatgatg aatggcactt cggcgacgtc tttgttttcg
     1861 cggacctttt gcagaagctg gtagcctgtc attggctcca tattccagtc cgaaatgatg
     1921 agtccatagg acttatcatg cattttttcg agtgcttcgg cgccgctcga tgcttcatcg
     1981 atatcggtga agccgatctg gccgagcagg ttacggatga tccgaaccat cgtcttgtag
     2041 tcatccacga tgaggatcgg aagcgacggt tctacaatca ttaacggcct ccggccggat
     2101 aaaaacggtc ggttaataaa aagcggtgct gcgccgccaa atcagcttgg tcgatgccgc
     2161 cgcgatta
//
LOCUS       DASWOG010000032         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_312365,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000032 DASWOG010000000
VERSION     DASWOG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312365"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VGB93_00605"
     CDS             <1..1022
                     /locus_tag="VGB93_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0144661.1"
                     /translation="GFYKHGQRFPIAFDIGAIDVPWTLDEPMGSNDIMFMERSSSQVI
                     ATKFGGGDNRTAIGFRSNDDRYWVGGYLTGPTTGSLHTAVAQVGAPLGETGLGPQFAG
                     LFRATYQLYQDKATNASLHIGFNFADTFDPRAGSNAEGFSLSDQPELRVDPTTILGTG
                     TVNAHGGQVFGGEAAAAYGNAFIQGEYYHYVIDTKNSATYAGTNGPAVGFNGGYVQGS
                     YTFGGRRYYKPTTGAYSGVIPDRPLVFGTDGWGALEIAGRFSIVDLNSSTHVPVLGSA
                     ATTQILGGQQTSFGAGANWYPNNNIKFMLDYEHVIENNPTIFAGPNTKGGTIDWIAAR
                     SQVVF"
     gene            complement(1093..1320)
                     /locus_tag="VGB93_00610"
     CDS             complement(1093..1320)
                     /locus_tag="VGB93_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144662.1"
                     /translation="MKMSLLMSLIAVALSSGVAFADGEYHHRHHHVAYSRSENVSGQY
                     YDLYHAPLNATPAYNDPYGVHSVLPSPVSDQ"
     gene            complement(1547..2707)
                     /locus_tag="VGB93_00615"
     CDS             complement(1547..2707)
                     /locus_tag="VGB93_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0144663.1"
                     /translation="MAASEAEDAEAFHVWVFSDAHVATDKAVSDAIRNGMEFIPPAGY
                     PESLANALRQSEFGSELGGPPFRWDIALDLGDNAGLWDLPDDRQGAEVVRQFGALKQH
                     RREQIYPVAGNHDASPGDAESSRGKPANWWFRKWVDPLSENPDFSGVEAARRPYPIEG
                     TWERYGFRVGNLRFLMMSDRNDLPYPVGRRETGGGSPAGAVTSDTFAWWQREVEAAGD
                     DIVISCHHHMLRETTVASGDYEGVSRYPDGRWRHGRYHGVDGVPEGASYLYFIDDEPK
                     AQKFEAYLAAHPGAVDLWLGGHTHTFPEDVVNGRSHVETKWSTHFVNCAQLSKYHSFV
                     TCPPMSRHFIFTPGSRQLRARCYLHDDSFAAQGWYVPGERVLELSAPFLPHP"
     gene            complement(2734..>3432)
                     /locus_tag="VGB93_00620"
     CDS             complement(2734..>3432)
                     /locus_tag="VGB93_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0144664.1"
                     /translation="NPLLFPSFTETVSALLQSAGSEDLLSSAASSLLELFKGYTLALS
                     IALVLVSLAVTLPFMRDVLQTLTAIFNPLPAIALLPLAMLWFGLGEASLLFVLVHAVV
                     WPFSLAALAGFEQVPEAQRLVGRNYGLRGPRYILYILLPAALPSILSGLRVAWAFAWR
                     TLIASELVFGVSSGTGGLGWFIYRNRNDLLTDRVFAGLATVILIGLFVEFAIFHLIER
                     RTVERWGMQRPAAR"
BASE COUNT          773 a          993 c         1015 g          651 t
ORIGIN      
        1 acggcttcta taagcatggg cagcgattcc cgatcgcctt cgatattggc gccatcgacg
       61 tgccgtggac cctcgacgag ccaatgggtt cgaacgatat tatgttcatg gagcgctcca
      121 gctcacaggt tatcgccacg aagttcggcg gcggtgataa tcgcacggcg atcggcttcc
      181 gctccaacga cgaccgttat tgggttggcg gctatctgac cggcccgacg accggttcgc
      241 tgcatacagc tgttgcgcag gtgggcgcgc cgctgggtga aacgggtctc ggaccgcaat
      301 tcgctggcct tttccgcgcg acttaccaac tttatcagga caaggcgacg aatgcttctt
      361 tacacatcgg cttcaacttc gccgatacat tcgatccgcg ggccggctcc aatgccgagg
      421 gcttctcgct gtccgatcag cccgaactgc gtgtcgaccc gacaaccatt ctgggcaccg
      481 gtacggtcaa cgcgcatggc ggtcaggtct tcggtggcga agctgccgca gcctacggca
      541 atgccttcat ccagggcgaa tattatcact acgtgatcga tacgaagaat tcggcgactt
      601 acgcggggac caatggtccg gcggtcggtt tcaacggcgg ctatgtccag ggcagctata
      661 cgttcggcgg ccggcgttat tacaagccga cgactggcgc ttattcggga gtgatcccgg
      721 atcgcccgct ggtgtttggc acggacgggt ggggtgcgct tgaaatcgcg gggcgcttca
      781 gcatcgtcga tctgaattct agcacgcacg ttccggttct cggcagcgcc gctaccaccc
      841 aaatcctcgg cggccagcag acgtcgttcg gcgccggtgc gaactggtat ccgaacaaca
      901 acatcaagtt catgctcgat tatgagcacg tgatcgaaaa caacccgaca atcttcgctg
      961 gcccgaacac aaagggcggc acgatcgact ggatcgcggc ccgttcgcag gtcgtgttct
     1021 aaacaaaagc tacaaacatc cacgaagccc ggcaggtcac gcttgccggg cttttttatt
     1081 taggctggca gtctactgat cggagaccgg cgacgggaga acgctatgga ctccataggg
     1141 gtcgttgtag gccggcgtcg cgttcagcgg agcgtggtag aggtcgtagt actgaccgga
     1201 aacgttttcg ctgcggctgt aagcaacgtg atggtgacga tgatgatatt cgccatccgc
     1261 aaaagccacg cccgacgaaa gtgcaacagc gatgagtgac ataaggaggg acatcttcat
     1321 aacgtttgcc ttctatctcg gtgcagcgcg caatgacgac cgcgcgccct gacctcaata
     1381 ttgctcggca aagcaattat tccatgccct atgttttaaa ttcggtttta tgtaatctca
     1441 tttactttca aatagaactc taaaacttat tttatactta cgctcgaagc tgaataccca
     1501 gagcgaaaaa cctgagcggc gcagagaaga tgcggatcgg cgcctttcac gggtgtggaa
     1561 gaaacggcgc gcttagctcc agcacgcgct cgccgggtac ataccacccc tgcgcagcaa
     1621 aactgtcgtc gtgcaagtaa cagcgcgctc gtagctgacg cgagccgggc gtgaagatga
     1681 aatgccggct catcggcgga caggtgacaa aggaatgata ttttgagagc tgcgcgcaat
     1741 tgacgaagtg cgtgctccat ttcgtctcga catgactgcg gccgttgacg acatcttcag
     1801 gaaacgtgtg ggtgtggccg ccaagccaga gatcgacagc gccaggatgc gcggcgagat
     1861 aggcttcaaa cttctgcgcc ttcggttcgt cgtcgatgaa ataaagataa gaggcgcctt
     1921 ccggcacgcc atcgacgcca tgatagcgcc catgccgcca gcgcccgtcc ggatagcgcg
     1981 aaacgccttc gtagtcgcca gaggcaaccg tcgtttcccg cagcatgtga tgatgacagg
     2041 agatgacaat atcgtcgccc gcggcttcga cttcgcgctg ccaccaagca aaagtgtctg
     2101 acgtcacggc gcccgcgggt gagccgccgc ccgtctcgcg gcgaccgacg ggataaggca
     2161 gatcgttgcg gtcgctcatc atgagaaaac gcaaattgcc gactcgaaaa ccgtaccgct
     2221 cccaggtgcc ttcgatggga taggggcgcc gcgcggcttc aacgcctgaa aagtccggat
     2281 tttcgctgag cggatcgacc cacttccgaa accaccaatt cgcgggcttg ccgcggctcg
     2341 attctgcatc gcctggcgag gcgtcgtgat tgccagccac gggatagatc tgctcacgcc
     2401 gatgctgttt cagcgcgccg aactggcgaa cgacttcggc gccttgtcga tcgtccggca
     2461 aatcccacaa tccggcattg tcgccgagat cgagcgcaat atcccagcgg aacggcggac
     2521 cgcccaattc gctgccgaac tcggactgac gcaaggcgtt ggcgagactt tcgggataac
     2581 ccgcgggcgg tatgaattcc atgccgttac gaattgcgtc cgacaccgcc ttgtcggttg
     2641 caacatgcgc gtctgaaaaa acccaaacgt gaaacgcttc cgcgtcttcg gcttccgatg
     2701 cggccaaagc aaaatctcca aattaggtga ggtttaacgt gccgcggggc gctgcatccc
     2761 ccagcgctcg acggtccggc gttcgatcag atggaagatc gcgaattcga cgaagagacc
     2821 gatgagaata acggtcgcga gcccggcaaa cacgcggtcg gtcaagaggt cgttgcgatt
     2881 gcggtaaatg aaccagccga gaccgccggt gccagagctg acgccaaaga caagctcgga
     2941 cgcgatcagc gtccgccagg caaaagccca agcgaccctg aggcctgaaa ggatcgaagg
     3001 cagcgcggca ggcaaaagga tgtagagaat ataacgcggg ccgcgcagcc cataattccg
     3061 tccgacgagc cgttgcgcct ccggtacttg ctcgaagccg gcgagcgccg ccaatgaaaa
     3121 cggccagaca acagcgtgga cgagtacaaa gaggaggctc gcttcgccga gcccgaacca
     3181 cagcattgca agcggcagaa gcgcgatggc gggcagggga ttgaaaatgg cagtcagtgt
     3241 ttgcagcaca tcgcgcatga atggcagcgt aactgccagc gagacgagga cgagcgcgat
     3301 cgacaatgcc agtgtgtagc ctttgaataa ttccagcaac gagctcgccg cagaactcaa
     3361 aaggtcttcc gaccccgccg attgaaggag ggcgctcacc gtttcggtga agctcggaaa
     3421 gagcagagga tt
//
LOCUS       DASWOG010000033         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_129059,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000033 DASWOG010000000
VERSION     DASWOG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129059"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGB93_00625"
     CDS             <1..879
                     /locus_tag="VGB93_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144665.1"
                     /translation="SKIISQDRGTPIEKIEKTVALGRLIGLLADHGAYDAAISAAQAI
                     PAINRNQYDLAVLRAAVDHKDAENVRRLPPLIIKAFDEQVAGGLPRQMELENLVLMLA
                     NGGYRDEARAIFNQLSAPSQGTISSPARWVQPSMLAELRAAIGELSGALQAANGAGPM
                     VTAPNARQILMLTAMQLAGHSHPTRDQVFAAVQRAKAALPSQVAGPKAWAFSAVASEL
                     AMTGDVANALEFEAKIEVEPRAVLSPARDAALSRIADAQMRTRDLRGAFVTAMRMSQG
                     PRWMKLLNLAGEPPRH"
     gene            complement(1510..>1775)
                     /locus_tag="VGB93_00630"
     CDS             complement(1510..>1775)
                     /locus_tag="VGB93_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144666.1"
                     /translation="LQEDLVRMIEMLQTAGKLLILPDVNKDELAAMLAAAVRGVNQTR
                     PHLKDVEFQPRYYAIIKAILRGSAKMPAKDRRRLEGVSSDIQS"
BASE COUNT          390 a          486 c          516 g          383 t
ORIGIN      
        1 tcgaaaatca tcagtcagga ccgaggcaca ccgatcgaga aaattgaaaa gaccgttgct
       61 ctcggccggc ttatcggcct tcttgctgac catggcgcgt atgatgctgc gatctccgcc
      121 gcacaggcga ttccggccat aaatcgcaat caatatgacc tggcggtatt aagagccgcg
      181 gtcgatcata aggatgctga aaatgtgagg agattgccgc ccctcattat caaggcgttc
      241 gatgaacagg tagcgggagg gttgccgcgg caaatggaat tggaaaattt ggttctcatg
      301 cttgccaatg gcggctatcg cgacgaagcg cgagcgattt tcaaccaatt gtcggcgccc
      361 tcgcagggta caatttcctc gccggccagg tgggtgcagc cttcgatgct agctgaactc
      421 agggctgcga taggcgagct ttccggtgcg ctgcaggccg caaacggggc tggtcccatg
      481 gtgaccgcgc caaacgcgag gcagatcctg atgttgaccg ccatgcaatt agccggtcat
      541 tcacatccga cgcgcgacca agtgtttgcc gcagtacagc gtgcgaaagc agcgctaccg
      601 tcccaagttg ctggtccgaa ggcctgggca ttctccgcgg tggcaagcga gcttgcgatg
      661 accggcgatg tagcaaacgc cttagagttc gaggcgaaga tcgaggtcga accgcgcgca
      721 gtgctgtcgc cggcaagaga cgcggcgtta tcccggattg ccgatgcgca gatgaggaca
      781 cgggatttgc gcggcgcttt tgtcaccgcc atgcgaatgt cccaaggccc gcgatggatg
      841 aaactcctga accttgcagg tgaacctcca cgtcactagc agccagtgtg atggatgcca
      901 aagtttgtcc gctgcgaacg aaagtgtgac cgtaaagcct acgaaaatat gtccgcacac
      961 ggaaaacgcc tcgaagtgac agttcgaggc gtttgtattt ttgaaacaaa aagaaagaga
     1021 tgaactttga aagaattgct ggccttcgcc cgattaacaa agccgctgca ttagctagcc
     1081 gtctcccgaa gccagcagtt tccgtcggct catgtcggat tctcttcggc agcctgagcc
     1141 ttcacgaacg gcacgagcaa gaacaaaagg ccgcacatca ggacgcgcgc tcgaagaaac
     1201 caactcatcg cactcactct cgcgagggcg cgctgtgccg cttacgcgcg gaagcgatgc
     1261 ccttgtcgtg cgaagagagt tggctctcga gtcactcggt tatatctcgt caatgggcgc
     1321 gtctattgtt cagggcgtgc tcaatgagcg gatgatgcgt tcgctagatt tgcgcgaacg
     1381 ttgttcagtc ttttttaaag ctgacaaagt gtaagcggca tgtccgttgg acgtgatgca
     1441 ttctgccggc tggatgagcg ggtggacggc aagtcgcgtc gcgcggcagc tcatcctcgt
     1501 catgcgtgct cagctttgga tatcggagct gacgccttca agtcgccggc gatccttcgc
     1561 aggcattttg gccgatccgc gcaatatggc tttgatgatc gcataatatc gcggttgaaa
     1621 ctcaacatcc ttcaagtgcg gacgcgtttg attcacgccg cggacggcgg ccgccaacat
     1681 cgccgccaat tcgtccttgt tcacatcggg caggattagc aatttgcctg ccgtctgtag
     1741 catctcgatc attctgacga ggtcttcctg cagcc
//
LOCUS       DASWOG010000034         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_202344,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000034 DASWOG010000000
VERSION     DASWOG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202344"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGB93_00635"
     CDS             <1..803
                     /locus_tag="VGB93_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018899586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144667.1"
                     /translation="RAANPLIEIRTFGNRNFSVGCALSFVVGMGLYGSIYLMPVFLAY
                     VRGHNALQIGEIMLVTGVAQIACAPIVAMLVRRFDVRFLSAFGFLVLTLGAALSANQT
                     QSTDFAGMFAGQLVRGCAFMFCVLPPTELALGNLTPAEIPDASGLFNLMRNLGGAVGI
                     AIIDSIIFTRSPEHAQKLFDGLVSGDTGTLSALGVTHSAVMQAATDPGKRAAMAELLK
                     KVAFADSINDAWFALAILTLAVTAALILARPSSGTHARTISRSFASTD"
     gene            complement(761..4222)
                     /gene="pyc"
                     /locus_tag="VGB93_00640"
     CDS             complement(761..4222)
                     /gene="pyc"
                     /locus_tag="VGB93_00640"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEY0144668.1"
                     /translation="MSVKRILVANRGEIAIRVFRAATEMGIATVAIYTDEDKLSLHRF
                     KADEAYQVGRGRGGELRLGPLESYLSIEEVLRVAVTAKVDAIHPGYGFLSESPEFAEA
                     CAAKGIVFIGPSPETMRTLGNKVAARNLAVSVGVPVMPATAPLPDDDAEIRRLAAEVG
                     YPLMLKASWGGGGRGMRPIESEDGLIDAVKSAKREAKSAFGKDEVYLEKLVRHARHVE
                     VQLLGDKHGTRVHLFERDCSIQRRHQKVVERAPAPYLGGATRAALCQAALKIGEATNY
                     IGAGTVEFLLDSDTGKFYFIEVNPRIQVEHTVTEQVTGLDIVKAQIKILEGGRLGYID
                     ETGIPPQDEIRLEGHALQCRITTENPDNNFIPDYGRITAYRGAFGFGIRVDGGTAYSG
                     AVVTRYYDALLEKVTAWAPSEAEVIQRMERALREYRIRGVATNLAFLEAVLNHPKFRD
                     GTYTTRFIDDTPELFATKKRRDRATKLLTYIGDVTVNGHPDTRNRPKPPAYARQADVP
                     QFPPRLAAEGSRDAFLRLGPENFAKWMKAQPQVLITDTTMRDAHQSPLATRMRTYDIA
                     AVAGAYAAGLPEVFSLECWGGATFDVAMRFLSEDPWERLQKIQQAAPSILTQMLLRGA
                     NGVGYTNYPDNVVRYFVRQAARAGMDLFRIFDCLNWVENMRVAIDAVAAEGKLVEGAI
                     CYTGDILDPDRAKYSLAYYVKLAKELEAAGCHILGIKDMAGLLKPAAARVLISTLKNE
                     TDLPLHLHTHDTSGISGATVLAAVITGVDAVDAAMDALSGATSQPCLGSLVEALRHTE
                     RDTGLDPAAIRRISFYWENVRRYYAAFESDQHHGASEVYLHEMPGGQFTNLKEQARAL
                     GLDARWHEVAETYRVVNDMFGDIVKVTPSSKVVGDMALMMVSQNLTRADVEAPHREIA
                     FPASVVDMLHGDLGQPPGGWPAALQKKALNDEPPITVRPGSLLKDIDFAAERANAEKA
                     CGRQIDDQEFASYVMYPKVFVEFAASQRKYGPVAVLPTLTFFYGMKLEDELSIEIEDG
                     KSLVVRLVALGETDDEGQVQVFFELNGQPRTIKVPNRSAVATRPARRKAEDGNDNQVA
                     APMPGLISMVSVKAGQAIKTGDVLLTIEAMKMETVLHAAKDATVKEILIAAGQSVDAK
                     DLLIVLA"
     gene            complement(4372..4626)
                     /locus_tag="VGB93_00645"
     CDS             complement(4372..4626)
                     /locus_tag="VGB93_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144669.1"
                     /translation="MTDREEANANSTRAPARGEANEKEDLPYRVTLWDEENIPVVKVL
                     ARATSIVLARAIYVAALTENPGRRITLNRGLETISDSLEL"
BASE COUNT          888 a         1477 c         1393 g          970 t
ORIGIN      
        1 tacgcgcggc aaatcctctg atcgaaatca gaaccttcgg taatcgcaat ttctcggtgg
       61 gctgcgcgct cagcttcgtt gtcggtatgg ggctctacgg atcgatctac ctcatgcccg
      121 tgtttcttgc ctatgtgcgc ggacataacg cgctgcagat cggcgagatc atgcttgtca
      181 ccggcgtcgc gcagatcgcc tgcgcgccga ttgtcgcaat gctcgtgcgg cgcttcgatg
      241 tgcgatttct tagtgctttt ggctttctcg tgctgacgct tggcgcagcc ttaagcgcca
      301 accagacgca gagcacggat ttcgccggaa tgtttgccgg gcagctcgtt cgtggctgcg
      361 ccttcatgtt ctgcgttctg ccacccacgg aactggcgct cggcaatctt actcccgcag
      421 aaattccgga cgccagcgga ttgttcaatc tcatgcgcaa ccttggtggc gcggtcggta
      481 tcgcaatcat cgattcgatc atattcaccc gttcgcccga acacgcgcag aagctcttcg
      541 acggccttgt atcgggcgat acgggcacac tctcggccct tggggtgacg catagcgcag
      601 tgatgcaggc cgccactgat cccggcaagc gcgcggcgat ggccgaactt ttgaaaaaag
      661 tcgccttcgc cgattcaatc aacgacgcct ggttcgcgct cgcgatcctg acgctggccg
      721 tcaccgccgc gctgatcctc gcgcgcccat caagcgggac tcatgcaaga acgataagca
      781 gatccttcgc atcgacggat tgaccggcgg cgatgaggat ttctttcacg gtcgcgtctt
      841 tggcggcatg aagcaccgtc tccatcttca tcgcctcgat ggtgagcagg acgtcgccgg
      901 ttttgatagc ctgcccggct ttgacggaaa ccatcgagat gaggcccggc atcggcgcgg
      961 cgacctgatt gtcgttgccg tcctccgcct tgcggcgtgc cgggcgtgtc gccaccgcgc
     1021 tgcgatttgg aaccttgatc gtgcgcggct gaccgttcaa ctcgaagaag acctgcacct
     1081 gcccttcatc gtcagtttcg ccaagcgcaa cgagacgcac aaccagactc ttgccgtctt
     1141 cgatttcgat cgaaagttcg tcttcgagct tcatcccgta gaagaaggtc agcgtcggca
     1201 agacggcgac cgggccgtat ttgcgctgcg aggcagcgaa ttcgacgaag acctttggat
     1261 acatcacgta agaggcgaac tcctgatcgt cgatctgccg tccgcaggcc ttctcggcgt
     1321 ttgcccgctc ggccgcgaag tcgatgtcct taagcagtga gcccgggcgt acagtaatcg
     1381 gcggttcgtc attcagcgct ttcttctgca aggcggcagg ccagccgccg ggcggctggc
     1441 cgagatcgcc atgcagcatg tcaacgaccg aagccggaaa tgcgatctcg cgatgcggcg
     1501 cttcgacatc ggcgcgggtc aggttctggc tcaccatcat cagggccatg tcaccgacga
     1561 ccttcgacga cggcgtcacc ttcacgatgt cgccgaacat gtcgttgacg acgcgataag
     1621 tctccgccac ttcatgccag cgtgcgtcga gtcccagcgc gcgtgcctgc tccttgagat
     1681 tggtgaattg cccacccggc atttcgtgca gatagacttc ggacgcaccg tgatgctggt
     1741 cgctctcgaa ggcggcgtaa tagcggcgca cgttctccca atagaaactg atccggcgaa
     1801 tcgccgcggg atcgagtccc gtgtcgcgct cggtgtgccg caatgcctcg acgagcgagc
     1861 cgagacaggg ctgtgacgtc gcgcctgaaa gcgcatccat tgccgcatcg accgcatcga
     1921 cgccagtgat gaccgcggcg agcacggtcg cgcccgatat gccggacgtg tcatgcgtgt
     1981 ggagatgcag cggcaggtca gtctcattct tgagcgtcga gatcagcacg cgcgccgcag
     2041 ccggcttgag cagaccggcc atgtccttga tgccaagaat atggcagccg gcggcttcga
     2101 gctccttcgc cagcttgacg taataggcca gcgaatattt cgcccggtcg ggatcgagaa
     2161 tatcgccggt atagcagatc gcgccctcga caagcttgcc ttctgccgcc acggcgtcga
     2221 tcgcgacgcg catattctcg acccagttga ggcaatcgaa aatgcggaaa agatccatcc
     2281 cggcgcgcgc cgcctgccgc acgaaatagc gcacgacatt atccggataa ttggtgtagc
     2341 cgacgccatt ggcaccacgc agcagcatct gcgtcagaat gctgggcgcg gcctgctgaa
     2401 tcttttgcag ccgctcccaa gggtcttcac ttaaaaagcg catggcgaca tcgaaggtcg
     2461 caccacccca gcattcgagc gagaaaacct ccggcagacc cgcggcgtaa gctccggcga
     2521 cggcggcaat gtcgtaggtg cgcatgcgcg tcgcaagcgg cgactggtgc gcgtcgcgca
     2581 tggtcgtgtc ggtgatcaga acttgcggct gcgccttcat ccatttggca aaattttctg
     2641 gccccagccg cagaaaagcg tcgcggcttc cctcggccgc gagcctcggc ggaaattgcg
     2701 gaacatcggc ttggcgcgca tacgccggcg gcttcggccg gttccttgta tcgggatggc
     2761 cgttgacggt gacatcaccg atataagtga gcagcttggt cgcgcggtcg cggcgtttct
     2821 tcgtcgcaaa aagctctggc gtgtcgtcga tgaaacgcgt cgtataggtg ccgtcgcgaa
     2881 acttcggatg attgagcacc gcttcgagaa aagcgagatt ggtggcaacg ccgcggatgc
     2941 gatattcgcg cagcgcccgt tccatccgct ggatgacttc cgcttcgctc ggcgcccagg
     3001 ccgtcacttt ctcgagtagc gcatcgtagt agcgcgttac aactgcaccg gaataggcgg
     3061 tgccaccatc aacccggatt ccgaatccaa aagcgccgcg ataggcggtg atgcggccat
     3121 aatctgggat gaaattattg tcgggatttt cggtcgtgat ccggcactgc aaagcgtgcc
     3181 cttcaagccg gatctcgtcc tgcggcggaa taccggtctc atcgatatac ccaagacggc
     3241 cgccttcgag gattttgatc tgcgctttga cgatatcgag ccctgtcact tgttccgtga
     3301 cggtatgttc gacctgaatg cgcggattga cctcaatgaa atagaatttt cccgtatcgg
     3361 aatcgagcag gaattccacc gtgcccgcgc cgatataatt tgtggcctcg cctatcttga
     3421 gcgccgcctg gcatagagcc gcacgcgttg cgccaccgag atagggcgcc ggcgcgcgct
     3481 cgacgacctt ctgatggcgg cgctggatcg agcaatcgcg ctcgaagaga tggacccgcg
     3541 tgccgtgctt gtcgccaaga agctgcactt cgacatgccg ggcatggcgc accagtttct
     3601 cgaggtaaac ctcatccttg ccgaaggcag atttcgcctc gcgcttggcg cttttcaccg
     3661 cgtcgataag gccgtcttcg ctttcgatgg gccgcatgcc gcgcccgccg ccgccccacg
     3721 acgccttcaa catgagcggg tagccgacct ccgcggcgag ccggcgaatt tcagcatcat
     3781 cgtcgggcag cggcgccgtc gccggcatca cgggcacacc gacggagacc gcaagattgc
     3841 gcgccgcgac cttgttgccg agcgtccgca tcgtctccgg cgacggcccg atgaaaacaa
     3901 tgcccttcgc cgcacaggct tcggcaaact ccggactttc cgaaaggaag ccatagcccg
     3961 gatggatcgc atcgaccttc gccgtcactg cgactctcag cacttcctca atcgaaagat
     4021 aggattccag cggtccaagg cgaagctcgc caccgcgtcc acggccgacc tggtaggctt
     4081 cgtcggcctt gaatcgatgc agggaaagct tgtcttcatc ggtatagatc gcgaccgttg
     4141 caattcccat ttcggtcgcg gcacggaaaa cccgaatggc gatctcgccg cgattggcga
     4201 ccaaaatccg cttgacgctc aaggctcagg ctcccgggag agtaattttt caacgagacc
     4261 ggagggcgcg ttgccgatcg gaagctgggc ctccgggccg gacctgaact ttacgctgag
     4321 cggccgcttc ctcaaactcc cccaaatcgc cgcacgcaag ggggcggtgc ctcacagctc
     4381 cagcgaatcg ctgatggtct ccagtccacg attcaaggtg atgcggcggc cgggattttc
     4441 cgtgagggcg gcgacataga ttgcccgcgc cagcacgatg cttgtcgcac gcgcgagcac
     4501 tttcacgacg gggatgtttt cttcatccca aagcgtgacg cgatagggta gatcttcttt
     4561 ttcgttcgct tcgccacgcg ccggcgccct ggtcgaattg gcattagctt cttcgcgatc
     4621 ggtcatttgc gtaacccgtc cccccgagcg gagaagcgaa caatcataag ctcacttaat
     4681 gcgccggccc gttccagctg gataatatcc tatgcttgtg gcgcaggg
//
LOCUS       DASWOG010000035         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_330706,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000035 DASWOG010000000
VERSION     DASWOG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330706"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB93_00650"
     CDS             <1..186
                     /locus_tag="VGB93_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0144670.1"
                     /translation="IGNLMGAISGPNFRVEGWFALLMYRSLYKMHEYALHGGRRVILS
                     ILSRGLSHRAEPEVKLH"
     gene            complement(218..1702)
                     /locus_tag="VGB93_00655"
     CDS             complement(218..1702)
                     /locus_tag="VGB93_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0144671.1"
                     /translation="MRSRVIHLINPRTDSLTTRPIYFNRALYSPLAGLLAVAACIPRD
                     QYEVILTDENIEEIDFDIEADLVGISAMTSYVNRGYEIADRFRAKGVPVVMGGVHPSF
                     MPKEALQHCDAVVIGEVEQIIGKVLDDLKDNAMRGTYRAAGLHPMVGVPMPRYDLLKK
                     NRYVNRTFVQTSRGCHQGCTFCAEPLMNGLKFRYRPVDEVIREMENCGERNISINDAD
                     FFGTPERPKELMRAMKGRGLQWQAGVTSKLAQDDEMLELAAESGCTLLSIGFESISRS
                     TLTSVHKHVNRPDQFAALVEKVHSYGIMVFGLFMFGFDGDDLDVFDETVKFNIDADYD
                     ACAYSVLTPYPGTLTWYELRKANRIVSYDWTMYDQGHVTYLPAQMESDELRVGHARAY
                     ENFYNLSSMAKRFPWAGKRKRAQWSVYNAFMRKAAKTDHIDSIAPPTADPTVAPMPPL
                     LPIKKEWRAAVLEAAGAPDPETAHLAPAPMSCDVGEDRPAAAAE"
BASE COUNT          407 a          599 c          655 g          443 t
ORIGIN      
        1 ataggcaatt tgatgggcgc gattagcggg ccgaacttcc gcgtggaagg atggtttgcg
       61 ctgctgatgt accgctcgct ctacaagatg cacgaatatg cgttgcatgg cggccggcgc
      121 gtcattctca gcatcctttc gcgcggtctc tcgcaccgcg ccgagcctga ggtcaagctg
      181 cattaaaaaa ggcgccgggt gatccggcgc ctttcaatta ttcggccgcg gcggcgggcc
      241 tgtcctcgcc gacatcgcag ctcattggcg ccggcgcgag atgcgcggtt tccggatccg
      301 gcgccccggc cgcttcgagc acggcggcgc gccattcctt cttgatcggt agaagcggcg
      361 gcatcggcgc gacagtcgga tcggcggtgg gcggggcgat cgaatcgata tgatcggtct
      421 tggcggcctt gcgcatgaag gcgttataga cgctccattg cgcccgcttg cgtttgcccg
      481 cccacgggaa tcgcttcgcc atcgacgaca aattgtagaa gttttcgtag gcacgcgcat
      541 ggccgacgcg caattcatca gactccatct gtgccggcag ataggtgacg tggccctgat
      601 cgtacatcgt ccagtcataa gagacgatgc gattggcctt gcgtagttcg taccaggtca
      661 acgtgccggg atagggcgtc agcaccgaat aggcgcaggc atcgtaatcg gcgtcgatgt
      721 tgaacttcac ggtctcgtcg aagacatcga gatcgtcgcc gtcgaagccg aacatgaata
      781 gaccgaagac catgatgccg taggagtgca ccttctcgac cagtgcagcg aactggtcgg
      841 gccgattgac gtgcttgtgg acgctggtca acgtcgagcg tgaaatcgat tcgaaaccaa
      901 tgctgaggag cgtacagccg ctctccgcgg cgagttccag catctcatcg tcttgcgcga
      961 gtttggacgt gacaccggcc tgccattgga ggccacggcc tttcatggcg cgcatcagct
     1021 ccttcggccg ttccggcgtg ccgaagaagt cagcgtcgtt gatcgagatg ttgcgctcgc
     1081 cgcagttttc catctcgcgg atgacctcgt cgaccggccg gtaacggaat ttcagcccgt
     1141 tcatcagcgg ctcggcgcag aaggtgcagc cctgatggca gccgcgcgat gtctgcacga
     1201 aggtgcggtt gacgtagcgg ttcttcttca gaaggtcgta gcgcggcatg ggcacaccga
     1261 ccatcgggtg caagccggcg gcgcgatagg tgccgcgcat ggcgttgtcc ttcagatcgt
     1321 cgagcacttt gccgatgatc tgttcgacct cgccgatcac gacggcgtcg caatgctgta
     1381 gcgcctcttt cggcatgaag ctcggatgga cgccgcccat gaccaccggc acgcccttgg
     1441 cgcggaaccg gtcggcgatt tcatagccac ggttaacgta gctcgtcatc gccgaaatgc
     1501 cgacgaggtc ggcttcgata tcgaagtcga tttcttcgat attctcgtcc gtcaggatca
     1561 cctcatattg atcgcgcggg atacaggccg cgacggcaag caggccggcg agcggtgaat
     1621 agagggcgcg gttgaaatag atcgggcggg tcgtgaggga atctgttctc ggattgatga
     1681 ggtgaatgac gcgtgagcgc aaaaggccct ccccggctcg actgaatgac agcacaggct
     1741 aacacgaagt tggtgttttg cctgtcaaat ttgccaaggt caatgtgacg ccccggcagt
     1801 aggcttgcgc gcatttgcaa cccagcttgg gcgcggcggg ctgcttaaaa catttatttg
     1861 ccgcctcagc cctgcctttc cccatccggg ccgacttcag aggccgccaa gggtttgcga
     1921 agaggggtgg ataatggata caatttatag ttgcgttaca tatatgaaag aaaaacggtt
     1981 ccgaattttc tcggcaatcg acaaatcagc ggccgtcgcc agcacgatct tacggccgcg
     2041 cgcggcttca ttttctgcca gcgtgacaag tgattggcga gccggaaagg tcgcgacatc
     2101 ttca
//
LOCUS       DASWOG010000036         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_184115,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000036 DASWOG010000000
VERSION     DASWOG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184115"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="purF"
                     /locus_tag="VGB93_00660"
     CDS             complement(<1..1237)
                     /gene="purF"
                     /locus_tag="VGB93_00660"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0144672.1"
                     /translation="MDVATEVPAEAIEAGSDYFPPLDLEADRLREECGVFGIFGHPSA
                     AAITALGLHALQHRGQEAAGIVSFDGHRFNSERRLGLVGEHFSKASTIERLPGRRAIG
                     HVRYSTTGETILRNVQPLFSEISSGGFAVAHNGNLTNGLTLRRNLVNDGAIFQSTSDT
                     EAILHLVARSRRPRLIERFIEALRQIEGAYSLVCLSNKKLIGACDPLGIRPLVLGELD
                     GCYILASETCALDTIGARFIRDIENGEIVVISESGIESLHPFPKRPARPCIFEYIYFA
                     RPDSVVRGRSVYDVRKAMGKQLARETAIDADVVGPVPESGVPAALGFSQESGIPFELG
                     IIRNHYIGRTFIQPTQSIRELGVRLKHSANRAAVAGKRIILIDDSIVRGTTSVKIVQM
                     MREAGAREVHFRISSPPITH"
     gene            complement(1454..>1684)
                     /locus_tag="VGB93_00665"
     CDS             complement(1454..>1684)
                     /locus_tag="VGB93_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase"
                     /protein_id="HEY0144673.1"
                     /translation="TGEELMAMLPDDPEGLLNKLKKAKPASEEPAPDSDTSPAPGATL
                     TPGSPSQPPHVAAPATPSPGTSAPAPAPQNKD"
BASE COUNT          328 a          483 c          549 g          324 t
ORIGIN      
        1 gatgcgtgat ggggggcgag gagatgcgga agtgaacttc gcgcgcgccc gcctcgcgca
       61 tcatctggac gatcttcacc gaggtcgtgc cacgcacgat cgaatcatca atgaggatga
      121 tccgcttgcc ggcgactgcg gcgcggttgg cgctgtgttt gagacgtacg ccgagttcgc
      181 ggatggattg ggtcggctga atgaaggtgc ggccgatgta atgattacga ataataccga
      241 gttcgaacgg gatgccggat tcttgcgaga agccgagtgc cgcggggacc ccggattccg
      301 gcaccggccc aacgacatcc gcgtctatcg ccgtttctcg cgcaagctgt ttgcccatcg
      361 ccttgcgcac atcataaacc gagcggccgc ggacgaccga gtcggggcgg gcgaaataga
      421 tatattcaaa aatacacggc cgcgccggcc gtttggggaa gggatggagg ctttcgatcc
      481 cactctccga gatcacgacg atttcgccgt tctcgatgtc gcggatgaaa cgcgccccga
      541 tcgtatcgag cgcgcaggtt tcagacgcga ggatataaca gccgtcgagt tcgcccagaa
      601 ccagcgggcg gatgccgagc ggatcacaag caccgatcaa cttcttgttg gacagacaaa
      661 ccagcgaata ggcgccttcg atctggcgca gcgcctcgat gaaccgttcg atcaggcgcg
      721 gacggcggct gcgcgcgacg agatgcagaa tcgcctcggt gtcggaggtc gattggaaaa
      781 tcgcgccatc gttgacgaga ttgcggcgca gtgtcaggcc gttggtcaga ttgccgttat
      841 gggcgacggc gaagccgcca gagctgatct cggaaaacaa cggctggaca ttgcgcagga
      901 tggtttcgcc ggtggtcgaa tagcggacgt ggccgatcgc gcggcggccg ggcaggcgtt
      961 cgatagtgga cgccttggag aaatgttcgc cgacaaggcc gagccggcgc tccgaattga
     1021 atctgtggcc gtcgaagctg acgatgcccg ccgcttcctg tccgcgatgt tgcagggcat
     1081 ggaggcctag cgctgtgatc gccgccgccg aggggtgccc gaagatgccg aaaacgccgc
     1141 attcttcgcg cagccggtcg gcttcaaggt cgagaggagg aaagtaatcg gacccggcct
     1201 cgattgcctc ggccggaact tcagtcgcca catccatgga cctgtctcgc ggttcgcttg
     1261 ggcagcgtga cgtcgtcgtc gacgattcac cgaattgtca cggcaatcac atagtatgta
     1321 gcgccgcgat taccaatgat tttaagcgcc cggtgccggg ttaaacccaa atatcgtcaa
     1381 tggtttcaca ccgcaacgag caccccttaa gtcgaaattt ccttacgaaa caagctctaa
     1441 cgcgtcctta cgctcaatcc ttattctgcg gcgccggtgc gggcgcagat gtaccgggcg
     1501 aaggtgtcgc aggcgcggcg acatgcggcg gctgggaggg cgaaccgggc gtcagcgtgg
     1561 cgcccggcgc aggcgacgta tcgctgtcgg gtgccggctc ttcgctcgca ggtttggctt
     1621 tcttcaattt gttcaggagg ccttctggat cgtccggcag catcgccatc agctcttcgc
     1681 cggt
//
LOCUS       DASWOG010000037         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_312564,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000037 DASWOG010000000
VERSION     DASWOG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312564"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1614..1688)
                     /locus_tag="VGB93_00670"
     tRNA            complement(1614..1688)
                     /locus_tag="VGB93_00670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1654..1656),aa:Thr,seq:ggt)
     gene            complement(1776..2663)
                     /locus_tag="VGB93_00675"
     CDS             complement(1776..2663)
                     /locus_tag="VGB93_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0144674.1"
                     /translation="MDKSKPSDDQSRWSAIKPVWLDLVQDVILSVSRNRLIANSGSVA
                     FFGLMAIFPAIATIVSLYGMFADPHTISNHLNLLTDVFPQSVIGLIRYQVMRVASRGN
                     GVQSLTFFVSFFVALWSANSGMSALFDALNVVYGETEKRSLPRFYGTTLTTTFASVIF
                     VVVALAGVVVLPVVWNFVGFHSSTDRLLDWLRWPVLFLIDTIGLDIVYRVGPSRPGAK
                     WRWLTWGSVFATFAWLGASILFSWYVAAFDSYDRVYGSLGAVIGFMTWIWVSVLIVLT
                     GAALNVELDRRRAAAKGAP"
     gene            2883..3440
                     /locus_tag="VGB93_00680"
     CDS             2883..3440
                     /locus_tag="VGB93_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144675.1"
                     /translation="MTDPIFKDIDAPHYAANESADEIARSAEAIVNPASGLANDYLNL
                     FNEIVMLIEQLPVMPELIDDILKWRPVTYQSYFAKSILPGRTSALEAYENLDLAFRRD
                     FETVVADLDRRAVGSVVAIRRQYKKQVNITSPMMAEICERAGAALREVLLTASDFVNN
                     GHRAERESEQERADRLIYGHLRRVG"
     gene            3544..4482
                     /locus_tag="VGB93_00685"
     CDS             3544..4482
                     /locus_tag="VGB93_00685"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0144676.1"
                     /translation="MLGADPAALAMAVDILRAGGLVAFPTETVFGLGADATSDAAVAK
                     IYAAKGRPAFNPLIAHVATRAAAEQQGVFSPQAIKLADAFWPGPLTLVLPLAEGSSVC
                     DLARAGLGSVALRVPSHPVAQRLLAVLGQPLAAPSANPSGRVSAVTAAHVREDFDGKI
                     DAIIEGSVNVGVESTIVACLADDPVMLRPGGIPRGEIEAVLGVKLRDAVPGAAINAPG
                     MVAAHYAPRARLRLDAESLEPDEAGLDFGGRFAPGSKVLDLSPRGDLTEAAAHLFAYL
                     RRLDERHPKIAVARIPSEGLGEAINDRLRRAAVGAR"
     gene            complement(4479..>4711)
                     /locus_tag="VGB93_00690"
     CDS             complement(4479..>4711)
                     /locus_tag="VGB93_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0144677.1"
                     /translation="PVGTDKAAFLARWGEVNEIVHGIVVKYHGSISAEHGIGRLKRDL
                     LPGVKDPAALDLMRTLKKTLDPKGILNPGKVL"
BASE COUNT          997 a         1358 c         1399 g          957 t
ORIGIN      
        1 acaattactt tccgtatggc ttcctggttt atatgcttct cggcgtcgcc tggttcttta
       61 tcctgaaaat gcgtgcgccc aaggtcttgc ttggtatcga gcatgacatg gaatccatgg
      121 ccacggcgga aacatttggc aagatggaag cagtgggtag cggagccgaa taagccgcaa
      181 ggtccaacga cggaaccgct tctcgctgaa acaacgccct ggggacaccc ccggggcgta
      241 tcttcatgat gctttgtctg gtccggcgag ttctgggtta cgccgttgcg cgatgattga
      301 tgggcggggc cgtcgtgcgg ccgtcgcgat cgcccggaaa ccgcttcgcc attcatgccc
      361 gttgcgcgtg cggattcgcg gatgcggata ccgcaaagag cacgctggaa atgcatgggc
      421 cgctgttgct gagccgaaat agggcaagaa aagccctcac aactgacaag gggtgccacc
      481 ccccggcggc cctttggacg cgcgaaacgc ggcagaaaca tctcaaacgt ctaaaacttc
      541 tgagtccaca ccttgcgcaa gaaaaatccg tcacacttgc gtaaatatgc ctgcctaaat
      601 acagtttaag agctatttta atcgagtttt gtctggagcg cgcaatagct tttaagtagc
      661 tactcgctgc tttcgcagct atttttgtca tcatttttag cgccaacgcc acccttgcct
      721 gtcacgcgca atgcacgccg ctgctggacg agaatttcca gaagccggac acggggtgga
      781 atacagtgag cggcaacatc tggtctacaa gtccgaaagg tctggtggcc gttgtcccaa
      841 ccaactcgac tcaatcgaat tggaatacaa acttcacctt ggacggcacc gactggtgtg
      901 tggaagtaag ttttcctgca accatgccaa cgcccgcaaa ctatgacacg gtgggcgcct
      961 gctggcattg agttttggag gaaagaccaa aaccattttt acgtcgctga agtttccccg
     1021 gacggttccg tcgtggttaa gcgagtcatc ggtggcaatt ggtcgacgat ttttaggcaa
     1081 tcgccgtcgc cgtccgtgaa gaagggcgca agtgtgacca acgaagttga aatacaaact
     1141 aatgggccat ccggaatact gtgggtcaac gggacccaac tcacgacctt ccgcggtcag
     1201 ccgccagctg atggagggaa tcccgcgctc atcggcgaat ccgccgacaa aacgccgacg
     1261 acttggactt tccctcgcac ccgagtcttc acagctcctc aatagccgtg ggcactttgt
     1321 cttgcctctc aagaatgctg taacatcgcg acggaacgcc ggtggtgtta caaccgctac
     1381 gatagaccac cggttatcag cggaggattt gccgcggatc gcctctactg ggcaacagga
     1441 acgactgagc ggctgtggtt gctgagctga aagcgatagc cgcagacatc gctccaagcc
     1501 cacgcatggc aatatcgccg ttgagacaaa tctagtctga tttttgagtc gatctcgccc
     1561 taagacggct gcggcgataa cgcaagaagt tgatgtaatt caagtggtta tggtggtgct
     1621 gcaagagagg attgaactct cgacctctcc cttaccaagg gagtgctcta ccactgagct
     1681 actgcagctt attgccgacg gtcaggcgct gacggtcgag gcgggggcta tgtcgcatag
     1741 ccgacgccga taggcaagac ggctcacaag acggctcaag gggcgccctt ggctgccgcc
     1801 cgccggcggt caagttccac attgagggcg gctccggtca ggacgatcaa gaccgagacc
     1861 cagatccagg tcatgaagcc gatgacagcg cccagcgaac catagacgcg gtcgtagctg
     1921 tcgaaggccg cgacatacca ggaaaagaga atcgaggcgc cgagccaggc aaaggtcgca
     1981 aacacgctgc cccatgtcag ccagcgccat ttagcgcccg gccggcttgg gccgacccgg
     2041 tagacgatgt cgagaccaat cgtgtcgatc aagaaaagaa ccggccagcg cagccagtcc
     2101 agcagcctgt ctgttgaaga atggaagccg acgaaattcc agacgaccgg gagaacgacg
     2161 acgccggcga gcgcgaccac gacaaaaatg accgacgcga atgtcgtggt cagggttgtg
     2221 ccgtagaacc tcggcagact gcgcttctcc gtctcgccat agacgacgtt cagtgcatca
     2281 aataatgcgc tcatgccgga attggcgctc cagagcgcga cgaagaagct gacaaagaag
     2341 gtcaggctct gtacgccatt gccgcggctc gcgacgcgca tgacctggta gcgaatgagc
     2401 ccgatgacac tttgcggaaa cacgtccgtc agcagattga gatgattcga gatcgtatgc
     2461 ggatcggcaa acatgccgta gagcgagaca atcgtggcga tcgcgggaaa aatcgccatc
     2521 aggccgaaga aggcgacact gccggaattc gcaatgagcc ggttgcgact gaccgacagg
     2581 atgacatcct gcacaagatc gagccacact ggcttgatgg ctgaccaccg gctttggtcg
     2641 tcacttggtt tggacttatc cattggccgg tcggtatgaa ggggtcgatt aattaacaac
     2701 gatctgcggc gtctgtcgtt cggctagcgc ggagaatcac tcggatttta ggtaacattg
     2761 tataggtcgt cgcgctttgc ccttcggcga gaaaaaactt ttcgtctcaa gacggtaagg
     2821 aaacttcttg ttatccttct accttcaaaa tggaccacac tggtaaacgc cgagccggct
     2881 gcatgaccga ccctatattt aaagatatcg acgcccccca ttacgctgcc aatgagtccg
     2941 cggatgaaat cgcccgcagc gcggaggcga tcgtcaatcc ggcgtcgggc ttggcgaacg
     3001 attatctcaa cctgttcaac gaaatcgtga tgctcattga gcaattgccg gtaatgccgg
     3061 aattgatcga cgatatcctt aaatggcggc cggtgaccta tcagtcctat ttcgccaaat
     3121 cgattttgcc gggccggacg tcggcacttg aagcttatga aaacctcgac cttgcgttcc
     3181 ggcgcgattt cgagacagtc gtcgctgatc tcgaccgccg cgccgttggt tccgtggtcg
     3241 cgatccggcg gcaatataaa aagcaagtca acatcacgtc gccgatgatg gccgaaatct
     3301 gcgagcgtgc cggcgcggct ctgcgcgagg tgctgctcac cgcgagcgat ttcgtcaaca
     3361 acggtcatcg cgccgaacgc gaaagcgagc aggagcgggc cgatcggctg atctacggcc
     3421 atttgcgccg ggtcggctga gctgctcacc cgttcttgac gtaatctggc aaatctctac
     3481 ctctccgccc ggccgcaacc tcgcggcccg atgcaacgag caggcagttt gaacacgacc
     3541 ctcatgcttg gtgccgatcc ggccgcgctc gcaatggcgg tagatattct gcgcgctggc
     3601 ggccttgtcg ctttcccgac ggagacggtc tttggcctcg gcgccgatgc aacctcggat
     3661 gcggcagtcg caaaaattta tgccgccaaa ggccggccgg cttttaatcc gctgattgcc
     3721 cacgttgcaa cgcgcgctgc ggcggagcag cagggggtat tttcgccgca ggcaataaag
     3781 cttgcggacg cgttctggcc ggggccgctc acgcttgttt tgcctctggc ggagggctcc
     3841 agtgtctgcg atctcgcccg ggccgggctt ggcagcgtcg ccttgcgggt tccatcgcat
     3901 cctgttgcgc agcggcttct ggctgttctg gggcagccgc tggccgcgcc ttcggccaat
     3961 ccgtcgggcc gcgtcagtgc ggtgacggcg gcgcatgtac gcgaagactt cgacggcaag
     4021 atcgatgcga tcatcgaggg cagtgtcaat gtcggggttg agtccacgat cgtcgcctgc
     4081 ctggcggacg atcccgtaat gttgcgtcca ggcggcattc cgcgcggtga gatcgaggcc
     4141 gttctcggcg tcaagcttcg ggatgccgta cccggagcgg ccatcaatgc ccccggcatg
     4201 gtcgcggctc attatgcgcc gcgcgcccgg ctacggcttg atgcggaaag cttggaaccg
     4261 gacgaagcgg ggctcgattt cggtggtcgc ttcgcgccag gctccaaagt cctcgatctg
     4321 tcgccgcgcg gcgacctcac agaggccgcg gcgcatctct tcgcttatct gcgcaggctt
     4381 gatgagaggc atccaaagat tgccgttgcg cggataccgt cagaaggcct tggcgaggcg
     4441 atcaacgacc ggttgcggag ggcggcggtc ggcgcgcgct agagcacttt gcccgggtta
     4501 aggatgccct tcgggtcgag cgtcttcttc aaagtccgca tcaggtcgag tgccgccgga
     4561 tctttgacgc caggcagcag atcgcgtttc agccggccga tgccgtgttc ggccgagatc
     4621 gaaccgtggt attttacgac gatcccgtga acgatctcgt tgacctcacc ccatcgcgcg
     4681 agaaacgcgg ctttgtcggt gccgacaggc t
//
LOCUS       DASWOG010000038         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_110905,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000038 DASWOG010000000
VERSION     DASWOG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110905"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..276)
                     /locus_tag="VGB93_00695"
     CDS             complement(13..276)
                     /locus_tag="VGB93_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0144678.1"
                     /translation="MHNSAEIGWIGAIIIGGLAGWFAEMFMGSGTGILLNIILGIVGA
                     AVANFLLGLLGIGLGGWIGYLIAGFIGACILIGLWRLIQGRRV"
     gene            complement(493..627)
                     /locus_tag="VGB93_00700"
                     /pseudo
     CDS             complement(493..627)
                     /locus_tag="VGB93_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            764..946
                     /locus_tag="VGB93_00705"
     CDS             764..946
                     /locus_tag="VGB93_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144679.1"
                     /translation="MPNQLVRATATGLPTEPAFTLTDAGRAYLLLEDIAAASVDLPPD
                     IAAELTALTAGGCDHV"
     gene            966..1469
                     /locus_tag="VGB93_00710"
     CDS             966..1469
                     /locus_tag="VGB93_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144680.1"
                     /translation="MNEDADLQASALLTMLLDRFEMDVDQYGAVELRASLNPTESDWL
                     CAWGAANEDVEDDDAAEEDMPPEESENLESDSRIRFGAGEGTTVALHQPTSFSDGDRV
                     FSKIDRDGRPAKVLGVVASWRYESMTSTIYQTIQPDDGSRPFEVDESHVRSRSGHKRP
                     RAAPIRS"
BASE COUNT          348 a          464 c          464 g          330 t
ORIGIN      
        1 ggttggactg gtttagactc gccgaccttg aatgaggcgc caaaggccaa tgaggatgca
       61 ggcaccgata aagccagcga tcaaatagcc gatccaaccg cccaatccga tacccagtag
      121 gccgagtaga aagttggcca cagcagcacc gacgatgcca agaatgatgt tcagaagtat
      181 gcctgtgccg gatcccataa acatttcggc gaaccagccg gccaaaccgc cgataatgat
      241 cgcaccgatc cagccaatct ccgcactgtt atgcattgct tctctcccac atggatggca
      301 ccgggcatca cgcggggtgc tttgttttgc ccaaagcgct tcaacgcttt ccgcaatttc
      361 acgacgtctt cgccgctttc gctagatgat ggtccgactc ttcaacgcca cgcccgcgcc
      421 gagcgcttca gtgccaactt caagaaactg cacgccggct ttttcaaggg tggtcttgat
      481 tgtcatggcc gttgataggc gcggatccgt gacgccgcgt tctatgtttt taatgctctg
      541 tcgagcgagt ccgctcgcct cggctagctc gtcttgtgac aagtttagta gggcgcgcgc
      601 ggcgcgaatc tggacggggg taatcatagc ttcattggcc gcagagccga cctagttgtc
      661 aacaaatatg atcctgtaga tcataaacgt ggttgacgag cttagctgtt tggcattaaa
      721 actgatccgt tagatcagaa taatggcacg aggggttatt gaaatgccga accaacttgt
      781 tcgggcaacc gctaccggct tgcctactga acccgccttc acccttaccg acgcgggccg
      841 cgcctacctg cttttagaag atatcgccgc tgccagcgtc gacctgccgc ccgatatcgc
      901 cgccgagttg acggcgctca ccgcgggagg ctgcgaccat gtgtgaagcg tgcgctctaa
      961 ggaaaatgaa cgaagacgcg gaccttcagg cgtccgcgct tctcacgatg ctgctcgata
     1021 ggttcgagat ggacgtcgat caatacggcg ccgtggaatt gcgggccagc ctgaacccaa
     1081 ccgaatccga ctggctctgt gcgtggggtg ccgcgaacga agacgtcgaa gacgacgacg
     1141 cggccgaaga ggacatgccg cccgaggaaa gcgagaacct tgaatcggac tcgcggatca
     1201 ggttcggggc aggtgaggga acgacggtgg ccttacatca gccgacctcg ttcagcgatg
     1261 gcgaccgcgt cttttcaaag atcgatcgtg acggacggcc ggccaaagtt ttgggagtcg
     1321 tggcttcgtg gaggtatgag tcaatgacct ctacgatcta ccaaacgatt caacccgacg
     1381 acggctcgcg gcctttcgag gtagacgagt cgcatgtgcg atcgagaagc gggcacaagc
     1441 gcccgcgcgc agcgcctatt cgcagctaac gatgagggca gggcgcttta ccatcgtgcc
     1501 cgacgccgcc aaaaggcccg cacatcgcgg gcctttttca ttgcctagcg ttgccgaggc
     1561 tttcgcttcg ccgtggcgat tcggtggcga ttcgcaaaga attgtc
//
LOCUS       DASWOG010000039         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_969, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000039 DASWOG010000000
VERSION     DASWOG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VGB93_00715"
     CDS             complement(<1..1043)
                     /locus_tag="VGB93_00715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0144681.1"
                     /translation="MTFRELGLSEKVLQAVEASGYTTPTPIQAQAIPEALAGRDILGI
                     AQTGTGKTAAFVLPMLSRLEQGRARARMPRTLILEPTRELAAQVEESFVRSGINHKLN
                     VAILIGGVSFGDQETKIMRGADVLIATPGRLLDFAERGKLLLTGIEILVIDEADRMLD
                     MGFIPDIERICKLVPFTRQTLFFSATMPAEITRLTEAFLHNPIRIEVARASSTSVTIA
                     QTVVASLPGPAKRETLRRLIRSAADNFKNAIIFCNRKRDVAILHKSLEKHGFRAGALH
                     GDMDQRARMASLEAFKNGDVDLIVCSDVAARGLDIPDVSHVFNFDVPVHAEDYVHRIG
                     RTGRAGKLGIAIS"
     gene            complement(1301..2134)
                     /locus_tag="VGB93_00720"
     CDS             complement(1301..2134)
                     /locus_tag="VGB93_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY0144682.1"
                     /translation="MFAALIIVFREVVEAGLVVGVVLAVTQNVPGRLQYVFGGVAAGI
                     GGAGIVALFTGLIAEALAGSGQEVLNASVLGIAVVMLTWHNVWMARHGREMTQELSDF
                     GQQVSSGSKSLFALAIVVAVAVLREGSEILLFLYGVLVSGGTTVLNVFFGGVLGLIGG
                     ALVAYLTFRGLITIPMRHLFRVTTLLISFIAAGMAAQAIGFLDQADIVTTLDRTVWDT
                     SWLLADDSWVGRVAHTLLGYTDQPTELQLIVYVTVLAVIFVLMKVFAPPRRPVAQLAA
                     N"
     gene            2404..3753
                     /gene="glmU"
                     /locus_tag="VGB93_00725"
     CDS             2404..3753
                     /gene="glmU"
                     /locus_tag="VGB93_00725"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY0144683.1"
                     /translation="MSARKRTCLAIVLAAGEGKRMRSARPKVLHELAGRSLLAHVLTT
                     VAAAGAERVALVVAPGHDSVVKEAKSLAPDIEVFVQSPALGTAHAVLAAKAAIAERYD
                     DILVAFADTPLVRPETFARLREGLTEEGRAVAVLGFRASNPRGYGRLIQDNGTLVAIR
                     EDRDLQAGEAEITFCNAGLMAIAGATALSLLESIDCSNAQGEYYLTDIVAAARQRGHG
                     AIAVEAPEDEVLGINDRAQLSVAEQVFQNRLKIRAMQSGVTFIDPSRVTLSYDTQFGR
                     DVLVEPDVFFGREVKVGDGVTIRAFSHLNGADIGPNCIIGPFARLRPGAKLAEDVHVG
                     NFVEVKASTLGAGVKANHLAYIGDATIGAKTNIGAGVITCNYDGFGKFQTDVGEGVFV
                     GVNSALVAPVKIGDGAYIGTGSVITDDVAPDALALARSRQVEKPSWAKDFRAKHRKT"
     gene            3790..5625
                     /gene="glmS"
                     /locus_tag="VGB93_00730"
     CDS             3790..5625
                     /gene="glmS"
                     /locus_tag="VGB93_00730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0144684.1"
                     /translation="MCGIVGILGHGPVAGAVVEALSRLEYRGYDSAGIATLEDGKLTR
                     RRAAGKLKNLEALLAKEPLHGHAGIGHTRWATHGKPTPENAHPHRNGRVAVVHNGIIE
                     NFRALREELSAKGHAFESETDTEVVAHLVGESLAQGLSPVEAVAECLPRLKGAFALAF
                     LFEGEDNLLIGAREGAPLAVGFGTGQREGEVYLGSDAIALAPLTDQLVYLEEGDYTVL
                     TRQGAKFFDAANLPVERKRITTPASAFLFDKGNYRHFMAKEIHEQPEVVGRTLSHFLD
                     LSSGTVSLPFELPVDATTLPRLTIAACGTAYMAGLVASYWFERIARLPVEIEVASEFR
                     YREAPMQKGGLMLLISQSGETADTLASLRYAKSQGQKILSVVNVPTSTMARESDVAAQ
                     TLAGPEIGVASTKAFTCQLALLACLVLAFAKARGTIDAKEEARLVAELTAVPGLIAET
                     LKHEAQVELLARDLSKARDVLYLGRGTSYPLALEGALKLKEISYIHAEGYAAGELKHG
                     PIALVDSDLPVIVLAPRDRVFEKTLSNVQEVAARNGRIILISEAEAAHELGVPADIIA
                     MPKMAPDFSALVYAIPVQLLAYHTAVFMGKDADQPRNLAKSVTVE"
BASE COUNT         1122 a         1671 c         1819 g         1039 t
ORIGIN      
        1 atcgaaatcg cgatgccgag tttaccggcg cggccggtgc ggccgatgcg gtggacgtaa
       61 tcctcggcat ggacgggcac gtcgaaattg aagacgtgac tcacatccgg aatatcgaga
      121 ccgcgcgccg cgacgtcaga gcagacgatc agatcgacat cgccattctt gaaagcttca
      181 agcgaggcca tgcgcgcgcg ctggtccatg tcgccatgca aggcgccggc gcggaagcca
      241 tgcttctcca gcgatttatg caggattgcg acatcacgtt tgcgattgca gaagatgatc
      301 gcgttcttga aattatccgc agcagagcga atgaggcgcc gcaaggtctc gcgcttggcc
      361 ggaccgggaa gggaggcaac gactgtctgc gcgatggtga cggaggtgga agacgcccgg
      421 gcaacttcga tacggatcgg attgtgcagg aaggcttcgg tgaggcgggt gatttcggcc
      481 ggcatcgtcg ccgaaaagaa aagggtctgc cgggtgaagg ggaccagttt gcagatgcgc
      541 tcgatgtcgg gaataaatcc catgtcgagc atccggtcgg cctcgtcgat cacgaggatc
      601 tcgatgccgg tgagtagaag cttgccacgc tcggcaaaat cgagaagccg gcccggcgtc
      661 gcgatcagca cgtcggcgcc gcgcatgatt ttcgtttctt gatcgccgaa ggaaacgccg
      721 ccgataagga tggcgacatt tagcttatgg ttgatgccgg aacgcacgaa actttcttcg
      781 acctgagcgg cgagttcgcg cgtcggttcg aggatgagcg tgcgcggcat tcgcgctcta
      841 gcgcggccct gttcgaggcg cgacagcatc ggcaaaacga aagcagcggt cttgcctgtg
      901 cccgtctggg ctattccgag gatatctcgt ccagcgagcg cttcagggat ggcctgggcc
      961 tgaatgggtg tcggggtggt gtaaccggaa gcttctactg cttgcagaac cttctcgctg
     1021 agacctaatt ctctaaaagt catattaatc ctgaaagtag cgtcgactgc tcagcttgta
     1081 ttgagatgtg agcatcgcgc taacgaccaa ggcgcgttcc ggccctccgg agggaccgag
     1141 cggcaaaatt tggcgcctgg gtaaaatgcg gaaccgctct tgagagcgaa tctgtcggtc
     1201 cttgccgctc tttagccctg atatcggcag gatgcaaacg gattgcaagt cggcataggc
     1261 ccgcaaaagg tcacagacaa caaatccatg aaataaagga ttaattcgcg gccaattggg
     1321 caaccggccg gcgtggcggg gcgaaaacct tcatgagcac gaagatcacc gccagcaccg
     1381 tgacataaac gataagctgc aattcggtgg gctgatcagt atagccgagc aatgtatggg
     1441 cgacgcggcc gacccaggaa tcgtccgcca gcagccacga cgtatcccag accgtgcggt
     1501 cgagcgtggt gacgatatcc gcctggtcga ggaagccgat ggcctgggcc gccatgcccg
     1561 ccgcgatgaa cgagatgagc agcgtcgtca cccggaagag gtgacgcatt ggaatggtga
     1621 tcagaccacg gaaagtgaga taggcgacga gcgcgccgcc gatgagccca agcacgccgc
     1681 cgaaaaacac gttcaacacg gtcgtgccgc ccgagaccag cacgccatac agaaatagca
     1741 agatctccga accctcgcgc aacaccgcaa cggcaacgac gatggcgagg gcgaagagag
     1801 acttcgagcc actgctgacc tgctggccga aatcactcag ctcttgcgtc atctcgcggc
     1861 catgccgcgc catccagaca ttgtgccagg tcagcatgac gaccgcgatg ccgagcaccg
     1921 aggcattgag cacttcctgg ccggagccgg ccagcgcctc ggcgatcagg ccggtgaaca
     1981 gggcgacgat gccggcgccc ccgatgccag cggcaacacc gccgaagaca tactgtaacc
     2041 gccccggcac gttctgtgtc acggccagga cgacgccgac gacgagaccg gcttcaacga
     2101 cttcgcgaaa gacgatgatg agtgcggcga acaaagctga cctttttggg gcgggggcga
     2161 ttgatgtttt actatacggt tggaaagccc cgccgcaagg atgcgcgacg tcatttagaa
     2221 ctcgtcgaaa gcgctccgga atcaaggacg tttacaagaa tccgtgaacc cgcgctgctt
     2281 cggcgattcg gatataacgc ggccaatttg gatttgccgt aaagtctctg tatagcgcgt
     2341 ccgattcgaa agagatgagc tggcacatat gcagaccatg cattgcctgc ggcgggtgag
     2401 ccgatgagcg cgcggaagcg cacctgcctt gcgatcgtcc ttgcggcggg cgaggggaag
     2461 cggatgcgct cggcgcggcc gaaggtgctg cacgagcttg cgggccgctc tttgctcgcg
     2521 catgtgctca cgactgtcgc cgcggccggc gcggagcgtg ttgcgctcgt tgtcgcgccg
     2581 gggcacgatt ccgtcgtcaa agaggccaaa agtctcgcgc cggatattga ggttttcgtt
     2641 cagtcgccgg cgctcggcac cgcgcacgcc gtgcttgcgg cgaaggccgc cattgccgaa
     2701 cgctatgatg atattctcgt tgccttcgcc gacacgccgc tggtgcggcc ggaaactttt
     2761 gcgcgtctgc gcgaaggtct caccgaagag ggtcgcgctg ttgcggtcct cggcttccgg
     2821 gcgagcaacc cgcgcggtta tggccgcctg attcaagaca acgggacgct cgtcgcgatc
     2881 cgcgaagacc gcgacctgca ggcgggggaa gccgagatta ccttctgcaa tgccgggctg
     2941 atggcgattg ccggcgcgac ggcgctgtcg ctgctcgaat cgatcgattg cagcaatgcg
     3001 cagggcgaat attatctgac cgacatcgtt gcggcggcgc gccagcgtgg gcatggcgct
     3061 atcgccgtcg aagcgccgga agacgaggtt ctggggatca acgaccgtgc gcagctctcc
     3121 gtggccgagc aggttttcca gaaccgactc aagatcaggg cgatgcaaag cggtgtaact
     3181 ttcatcgatc cgtcgcgggt cacgctctct tatgacacgc agttcggccg ggacgtgctg
     3241 gtcgaaccgg acgtattctt cgggcgagag gtcaaggttg gtgacggggt cacgatccgc
     3301 gccttctcgc atctcaatgg cgccgacatc gggccgaact gcatcattgg gccgttcgcg
     3361 cgattgcggc ccggcgcaaa acttgccgag gatgtgcatg tcggcaattt cgtcgaggta
     3421 aaagcctcga ctcttggcgc tggggtcaag gccaatcacc tcgcctatat cggcgatgcg
     3481 acaatcggcg cgaagacgaa catcggcgcc ggcgtcatca cctgcaatta cgatggcttc
     3541 ggcaaattcc agacggacgt cggcgagggc gtcttcgtcg gcgtcaattc ggcgttggtg
     3601 gcgccggtga aaatcggcga tggcgcctat attgggacgg gatcggtcat cacggacgat
     3661 gtcgcgccgg atgcgctggc gctggcccgg tctcgccagg tcgagaaacc atcttgggcg
     3721 aaagattttc gcgccaagca ccggaagact taacctccct agactcgggc gagccggaaa
     3781 gtttgaagta tgtgtggaat tgtcggtatt ctcgggcacg gccctgtcgc cggagccgtc
     3841 gtcgaggcct tgagccggct cgaatatcgt ggctatgatt ccgccgggat cgccaccctt
     3901 gaggacggca agctgacgcg ccgccgcgca gcgggcaagc tcaagaatct cgaggcgctg
     3961 ctcgccaagg agccgctgca cggccatgcc ggcattggcc atacccgctg ggcgacgcat
     4021 ggcaagccga cgccggaaaa tgcccacccg catcgcaacg gcagggtcgc cgtcgtccac
     4081 aatggcatca tcgagaattt tcgggctctg cgcgaggaat tgagcgccaa gggtcatgcc
     4141 ttcgaatccg aaacggatac cgaagtcgtg gcgcatcttg tcggtgaatc tctggcgcaa
     4201 ggcctttcgc cggttgaggc tgtagccgag tgcctgccgc ggctgaaagg cgcgttcgcc
     4261 ctcgccttcc tgttcgaggg cgaggacaat cttttgatcg gcgcgcgcga gggtgcgccg
     4321 cttgccgtcg gtttcggcac gggccaacgc gagggcgagg tctatctcgg ctcggacgcg
     4381 atcgcgctgg cgccgctgac cgatcagctc gtctatctcg aagaaggcga ctacacggtg
     4441 ctcacgcgtc agggcgccaa gttcttcgat gcggccaatc tgcccgtcga gcgcaagcgg
     4501 atcaccacgc cggcgtctgc ctttctgttc gacaagggca attaccggca cttcatggcc
     4561 aaggaaatcc atgagcagcc ggaagtcgtc ggccgcacct tgtcgcattt cctcgatctt
     4621 tcatccggca cggtgtcgct gccattcgaa ctgccggtcg atgcgacgac gctgccgcgg
     4681 ctcacaatcg ctgcctgcgg cacggcctat atggccgggc tcgtcgcgtc ctattggttc
     4741 gagcgcattg ccagactgcc ggtcgaaatc gaggtcgctt cggaattccg ttatcgcgag
     4801 gcgccgatgc agaagggcgg cctgatgctg ctcatctcgc aatcgggcga gactgcggac
     4861 acgctagcga gcctgcgtta tgccaagtcg cagggccaga aaatcctcag cgtcgtcaat
     4921 gtgccgacct cgaccatggc gcgcgaaagc gacgtcgcgg cgcagacact tgccgggccg
     4981 gagatcggcg tcgcatcgac gaaggccttc acctgccaat tggcgctgct ggcgtgcctc
     5041 gtgctcgcct tcgccaaggc gcgcggcacg atcgacgcca aggaagaagc gcggctcgtc
     5101 gccgaactca ctgccgtgcc gggccttatc gccgaaacct tgaaacacga ggcgcaagtc
     5161 gagctgttgg cacgcgatct cagcaaggcg cgcgacgtgc tttatctcgg ccgcggcacg
     5221 tcctacccgc tggcgctgga gggcgcgctg aagctcaagg aaatctccta cattcacgcg
     5281 gagggctatg cggcgggcga gttgaagcat gggccgattg cgctcgttga ttcggaccta
     5341 ccggtgatcg tgctcgcgcc gcgcgatcgc gtgttcgaga agacactctc gaatgttcag
     5401 gaggttgcgg cgcgcaacgg acgcatcatt ctcatttcag aggcggaggc agcgcatgaa
     5461 ctcggcgtgc cggcggatat aatcgcgatg ccgaaaatgg cgcctgattt ctccgcactc
     5521 gtctacgcta tcccggtgca attgctcgcc tatcacacgg cggttttcat gggcaaagac
     5581 gccgatcagc cgcgcaatct ggcgaagagc gtgacggtgg aatagggcgc cgctcctccg
     5641 aaccggcttc t
//
LOCUS       DASWOG010000040         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_184193,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000040 DASWOG010000000
VERSION     DASWOG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184193"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGB93_00735"
     CDS             <1..958
                     /locus_tag="VGB93_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144685.1"
                     /translation="ESLRFITSFADIFVTLGIALFCWAAADITSHYVGDTAKWGVVAV
                     LAWGLAEFFARRRRMALPSIVLLLLFAIAIFKFVLGLVTPDDAGSYLVRRFEHFEGWA
                     NIDARSALIAGLATAAFAALHYWRFRVPITIAAGAAALCIAVVASAHWIDPSLELNIY
                     NGLLLLCGIGVFVLAMYFDMSDPTRQTRRTDIAFWLHLLAAPLIVHPLIAAFRQGAVL
                     DTQTSLIVLGIFLVFGIISLVIDRRALLVSGLAYAGIALSALLRETGFAGIAGLTSAT
                     LLALGLFILVLSAGWQPLRNAILRLLPSALTQRLSHPRTRLT"
     gene            955..2256
                     /gene="hslU"
                     /locus_tag="VGB93_00740"
     CDS             955..2256
                     /gene="hslU"
                     /locus_tag="VGB93_00740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY0144686.1"
                     /translation="MTDFSPREIVSELDRFIVGQKQAKRAVAIALRNRWRRLHLEGTM
                     RDEVMPKNILMIGPTGCGKTEISRRLAKLANAPFLKVEATKFTEVGYVGRDVEQIIRD
                     LLEIAIAQVKQGKRKSLQARAHLAAEERLLDALVGAHASPATRDSFRKKLRDGELGEK
                     EVEIELSQPSSNMPMFELPNVPGASFGAISIGDIFGKSKPSKPRRLLVNDAFEPLINE
                     ESDKMMDQDQIVQEAIREVENNGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIE
                     GTTVSTKHGAVKTDHVLFIASGAFHISKPSDLLPELQGRLPIRVELSPLDEEDFRRIL
                     VETEVSLIKQYEALLKTEGVELIFTPDGVNAIARIAVQVNSSVENIGARRLQTVMERV
                     LDEVSYSAPDRSGERIEINGEFVEKNIGDLARNSDLSRFIL"
     gene            2451..3539
                     /locus_tag="VGB93_00745"
     CDS             2451..3539
                     /locus_tag="VGB93_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144687.1"
                     /translation="MPATEITAASSTKTQMDSGANFFSRSRWQLVVFFNVLIVIAAFF
                     VLHAPFAYPLDDTYIHMAIARTLVQSHVWGVQSFEPAAASSSPLFTIILACFYRLVPN
                     DRLFYYVPLALNAAAMVAVIALWARMLRGQKYEFFALILLSIAVPAFVIPIIGMEHSL
                     QILIATCLAWRGAIALAQRDKSFATTAVIFVLSGLAVAARYEALALIGALLLLTMILR
                     NFSIAAALVFGALAAILGFGLIWVSQGGWLIPNSLLLKPLGDDGSLPVRVLSNLEHDT
                     TNIPGATALAVALCASAMAYWKYRKDIACRGRDRPGNRLALDLRGIRCAHDGRTASYR
                     NCRLSVSLRGLAALSRCAFCRTVTRVFV"
     gene            3562..4194
                     /locus_tag="VGB93_00750"
     CDS             3562..4194
                     /locus_tag="VGB93_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144688.1"
                     /translation="MVGLCLVTLPRAALALIDSVLAGDDRKLEHFATARFLSQNYRGK
                     TVVLNDIGIAAWEATDTRIADIFGLANNAVTAIRMKTHKFTSAQTEIWAKSNGADIAI
                     LEVCWQPLKGSIPKDWKFVALWRIPRNAVYRNFDIGFFAADPGAAARLESQIKAFAVD
                     KRVEVYYTDDRSSPIYGRKSADFHGTECNIHNSHGIYRRGIEIFDRPGAT"
     gene            4279..5313
                     /gene="trpS"
                     /locus_tag="VGB93_00755"
     CDS             4279..5313
                     /gene="trpS"
                     /locus_tag="VGB93_00755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0144689.1"
                     /translation="MAEFHPRVFSGVQPTGNLHLGNYLGAITKFVALQKTHQCLYCVV
                     DLHAITVPQDPAVLKASIRKVTAAYLAAGIDAKQQIVFNQSQVSAHAELAWILNCVAR
                     LGWLNRMTQFKEKAGKDRENVSVGLYDYPVLMAADILAYQATHVPVGEDQKQHLELAR
                     DIAQKFNLDFAESIAAHGHGDAFFPLPEPLIQGPATRVMSLRDGTKKMSKSDPSDYSR
                     INLDDDADLIAQKVRKAKTDPEALPSEVGGLVGRPELDNLVGIFAAVNDETKAQVLQT
                     FGGANFSTFKSALVDLLVAKLAPIGAEMSRLAKDETFIDGVLADGSARARAIAAPVVA
                     AVKGIVGFVR"
     gene            5391..6434
                     /locus_tag="VGB93_00760"
     CDS             5391..6434
                     /locus_tag="VGB93_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0144690.1"
                     /translation="MAENSRAISEPVLISFGQQGESFDAGHRLADGVPVGLRLTQNRA
                     RFPAFLSGSLALHLAVLAALIWTEHAPRFKPDVQQEIPVELVTDPAKDASSATKGKAK
                     PSGGPAEFKPGTAGKPVAPKPAEAKKPVAPPKPEAPKPEAAKPAAPKPAAPPPKPPEP
                     APAKPPAPKPPAPEPLAPTPKPPELKPSPPPEAQKQPPPAPQPVAPKPPQPSVLAAKP
                     PPPPIGPVGTPEGQPQGLMQDNSRAVAVPQPSDDGDDIVSYQTLVFGMLELKKEFPED
                     AKRRGVFGTAQIYFELNDDGSVKSEKLLKSSGDATLDVESLALVQRAAPFPKPPTGAQ
                     KVFAADITFPPPK"
     gene            complement(6466..>6843)
                     /locus_tag="VGB93_00765"
     CDS             complement(6466..>6843)
                     /locus_tag="VGB93_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0144691.1"
                     /translation="LIWDHLPPDLREEIDKKGRCFLPSSYSDWTYPITHELIEEGRKH
                     LILGGTIRTHCPVHILQGMKDVDVPWTHASLLVEHLAGDPVVLTLVKDADHRFSRPSD
                     LTLLAQAIDSVAGIPPQGELKLE"
BASE COUNT         1293 a         2066 c         2019 g         1465 t
ORIGIN      
        1 cgagagcctg cgtttcatca caagcttcgc cgatatattc gtgacgcttg gcattgcact
       61 tttctgctgg gcggcggccg acatcacctc gcattatgtg ggcgacacgg cgaaatgggg
      121 cgtcgtcgcc gtgctggcgt ggggcctcgc tgaattcttt gcccggcggc gtcgcatggc
      181 gttgccgagc atcgttctgc tgctgctctt cgccattgcc attttcaagt tcgttctggg
      241 gcttgtgaca ccggacgatg cgggctccta tctcgtccgc cgcttcgagc atttcgaagg
      301 gtgggcgaat atcgatgcgc gctcggcgct gatcgccgga ttggcaacgg cggcgttcgc
      361 agcgcttcat tattggcggt tccgcgtccc aatcacgatt gctgccggtg ccgcggcgct
      421 ctgcatcgcc gtcgtcgcct cggcccattg gatcgatccg tcgctcgaat tgaatatcta
      481 taatggtctg ctgctgctct gcgggatcgg cgttttcgtg ctcgccatgt acttcgatat
      541 gtccgaccca acccggcaga cgcggcggac cgacatcgca ttttggctgc atctcctcgc
      601 cgcgccgctg atcgttcatc cgctcatcgc ggcgttccgg cagggtgccg ttctggacac
      661 gcagacatcg ctcatcgttc ttggcatttt ccttgtcttc ggcattatct cgcttgtgat
      721 cgaccggcgc gccttgctcg tctccggcct cgcctatgcg ggcatcgcgc tcagcgcgct
      781 gcttcgcgaa accgggtttg ccggcatcgc cggattgaca agcgcgacgc tgctcgctct
      841 cggcctgttc atcctggtgc tcagcgctgg ctggcagccg ctgcgcaatg cgatcctgcg
      901 gcttctgcct tctgccctga cccagcgtct ctctcatccg cgcacacggc tcacatgacc
      961 gacttttccc cccgcgaaat cgtctccgaa ctcgaccgtt tcatcgtcgg ccagaaacag
     1021 gccaagcgtg ccgtcgcaat tgctctgcgc aatcgctggc ggcggctgca cctcgaaggc
     1081 acgatgcgcg acgaggtgat gccgaagaac attctgatga tcggcccgac cggctgcggc
     1141 aagacggaaa tctcgcgccg tttggccaag ctcgccaatg cgcccttcct taaggtcgag
     1201 gcgacgaaat tcaccgaagt cggctatgtc ggccgcgatg tcgagcagat catccgcgat
     1261 ttgctcgaaa tcgccatcgc gcaggtgaaa cagggcaagc gcaagtcgct gcaggcgcgg
     1321 gcccatcttg cggcggaaga gcggttgctc gatgcgctcg tcggcgcgca tgcctcgccc
     1381 gcaacacgcg attccttccg caagaaattg cgtgacggtg aattgggcga gaaggaagtc
     1441 gagatcgagc tgtcgcagcc atcgtccaat atgccgatgt tcgagctgcc gaatgtgccg
     1501 ggcgcctcgt tcggcgcgat ttcgatcggc gacattttcg gcaagagcaa gccgtcgaag
     1561 ccgcgccgcc tcctggtcaa cgacgccttc gagccgctca tcaacgaaga gagcgacaag
     1621 atgatggatc aggatcagat cgttcaggag gcgatccgcg aggtcgagaa caacggcatc
     1681 gtcttcctcg acgagatcga caagatctgc gcgcgggaag ggcgcggcgg cgccgatgtt
     1741 tcgcgcgagg gcgtgcagcg cgatctgctg ccgttgatcg aaggcacgac cgtttcgacc
     1801 aagcacggcg cggtcaagac cgatcatgtg ctcttcatcg cctcgggcgc gtttcacatc
     1861 tccaaaccgt cggacttgct gccggaattg cagggccgtc tgccgatccg cgtcgaactc
     1921 tcgccgctcg acgaggaaga tttccgccgt attctggtcg aaaccgaggt cagcctcatc
     1981 aagcaatatg aggctctgct gaagacggag ggcgtggaac tcatcttcac gccggacggc
     2041 gtcaatgcga tcgcgaggat cgcggtgcag gtcaattcga gcgtcgagaa tatcggcgcg
     2101 cggcggctgc aaacggtgat ggagcgcgtg ctcgacgaag tgagttattc ggcgcccgac
     2161 cgctcgggcg agcggatcga gatcaacggt gaattcgtcg agaagaatat cggcgatctc
     2221 gctcgcaaca gcgatctgag ccggtttatt ttgtaggcgc cgcatgttgc cacgcatgcg
     2281 ccttggccaa ttcataatgg ccgaataaaa gttgcctctt cgatttacta ttaatgaacg
     2341 ccttcccccg cgcggcttgt tcgcccttcg aacaatctgt accgttccgc agtgtcttca
     2401 acatcgaatg aattgaggtc acgatttatt cgagagggat gttgacgacg ttgcccgcaa
     2461 ctgagatcac agcggcctca tcgacaaaga cgcagatgga ttctggcgcg aatttctttt
     2521 cgcgcagcag atggcaatta gtagtcttct tcaacgttct gatcgtcata gctgcgtttt
     2581 tcgttctgca cgcgcccttc gcttatcctc ttgacgatac atatatccac atggcgatcg
     2641 cgcggacatt ggtgcaaagc catgtctggg gcgtccagtc attcgagccc gcggccgcat
     2701 cctcctcgcc gctcttcacc atcattctgg cttgcttcta ccgtctcgtg ccgaatgacc
     2761 ggctgtttta ttatgtgccc cttgcactca atgctgctgc gatggtggcc gtcatcgcgc
     2821 tttgggcgag aatgctccgc ggccagaaat atgagttctt cgcgctaatc ttgctgtcga
     2881 ttgcagtgcc tgctttcgtg atcccaatca tcgggatgga gcattcgctg caaatactga
     2941 ttgcaacttg tctggcatgg cggggagcga ttgcgcttgc gcagcgggac aagagcttcg
     3001 ccactacggc ggtgatcttc gttctttccg ggctggccgt cgcggcgcgt tacgaggcat
     3061 tggctttgat cggcgcattg ctattgctca ccatgatttt gcgtaatttt tcaatcgctg
     3121 cggcgctagt atttggcgcg ttagcagcga ttcttggatt tggtctgata tgggtgagcc
     3181 agggtggctg gctcattccg aattcgcttt tgctcaagcc gctgggggac gacgggagct
     3241 tgcccgtcag agtcctttcg aaccttgagc acgatacgac gaacataccc ggagcgacgg
     3301 cgctcgccgt cgcgctctgc gcttcggcaa tggcctattg gaaatatcgc aaggacattg
     3361 cgtgtcgcgg gcgtgacagg ccgggaaatc gattggcgct tgatcttcgc ggcatccgct
     3421 gtgctcacga cggccgcaca gcttcttatc ggaattgtcg gctttctgtt tcgttacgag
     3481 gcttggctgc tttgtctcga tgcgctttct gtcgtactgt tactcgggtc ttcgtttagc
     3541 gcgcgttttc aggcaatcgt tttggtgggt ctgtgcctcg tcacgcttcc acgcgcggcg
     3601 ctggctctta tagacagtgt tcttgctggt gatgaccgga agctggagca cttcgcgacg
     3661 gcgcgctttc tgtcccaaaa ctaccggggc aagactgtgg ttttgaatga catcggcatt
     3721 gctgcatggg aggcgaccga tacgcgcatc gccgatatct tcgggctcgc gaataatgcc
     3781 gtgactgcga tacgcatgaa gactcacaaa ttcacttcgg cgcagacaga aatctgggcg
     3841 aaatcgaatg gcgcggatat cgctatcctt gaagtctgtt ggcaaccgct aaagggctct
     3901 attcccaaag actggaaatt tgtcgcattg tggcgcatcc cgcgaaatgc cgtctatcgc
     3961 aatttcgata tcggcttctt cgcggccgat ccgggcgccg cagcgaggct tgaatcgcaa
     4021 atcaaagcat tcgcggtcga taaacgcgtc gaagtttact acaccgacga cagatcatca
     4081 ccgatttatg gcaggaagtc ggccgacttc cacggcactg aatgcaacat ccataactcg
     4141 cacgggattt atcggcgcgg gatcgaaatt tttgatcgtc caggcgcaac ttagcccgct
     4201 tgccagggcg gggtgggaag ctgcataaga cccgccacct tttcttatcg cattctggaa
     4261 cctggagcct cctcggccat ggccgaattt catccccgcg ttttctccgg cgtgcagccg
     4321 accggcaatt tgcatctcgg caattatctc ggcgcgatca ccaaattcgt cgccttgcag
     4381 aagacgcatc aatgcctcta ttgcgtcgtc gatctgcacg cgatcaccgt gccgcaggac
     4441 ccggctgtgc tcaaagccag catccgtaag gtcacggccg cttatctcgc cgcgggcatc
     4501 gacgccaagc agcagatcgt cttcaaccag agccaggtgt ccgcccatgc ggaactcgcc
     4561 tggattttga attgcgtggc acggctcggc tggctcaacc gcatgacgca gttcaaggag
     4621 aaggcgggta aggatcgcga gaatgtctcg gtcgggctct atgattatcc cgtgctgatg
     4681 gcggccgata ttctcgccta tcaggcgacg catgtgccgg tgggcgagga tcagaaacag
     4741 catctcgaac tggcgcgcga catcgcgcag aaattcaatc tcgattttgc cgagtcgatt
     4801 gccgcgcatg ggcatgggga cgcctttttc ccgctcccgg agccgctgat ccagggcccg
     4861 gcaacgcggg tgatgagcct gcgcgatggc accaagaaaa tgtcgaagtc cgatccgtct
     4921 gattattcgc gcatcaatct tgatgacgac gccgatctga tcgcgcagaa agtccgcaag
     4981 gcgaagaccg accccgaggc gctgccgagc gaagtcggcg gcctcgtcgg acgcccggaa
     5041 ctcgacaatc tcgtcggtat tttcgccgcg gtgaacgacg agaccaaggc gcaggtgttg
     5101 cagacctttg gcggggcgaa tttttcgacg ttcaaatcgg ctttggtcga tctgctcgtc
     5161 gccaagctcg ccccgatcgg cgcggagatg tcgcgcctcg ccaaggatga gacgttcatt
     5221 gatggcgttc ttgccgacgg ctcggcgcgg gcgcgggcga ttgcggcgcc ggtcgtcgcg
     5281 gcggtcaagg gcatcgtcgg cttcgtgcgc taaggcgtac gataagggat tttgtccggc
     5341 tacgctcttg ggctaggctt ggctgggtga tgtgatccga gagttggggc ttggccgaga
     5401 attcgcgcgc catatccgag cccgtgctga tctcctttgg acagcaaggt gaaagtttcg
     5461 atgcaggaca ccggctggcc gatggcgtgc cagttgggct gcgtctcacc caaaaccgcg
     5521 cgcgctttcc ggcttttctg tcgggcagtc ttgccctgca tctcgccgtg ctcgcggcgc
     5581 tgatctggac cgaacacgcc ccgcggttca agccagacgt tcaacaagag atcccggtcg
     5641 aactggttac cgacccagcg aaggacgcgt ccagcgcgac gaagggcaaa gccaagccgt
     5701 ctggcggccc cgccgagttc aagccgggaa cggcaggcaa gcccgtcgcg ccgaaaccgg
     5761 cagaagcgaa gaaaccggtc gctccaccaa aaccggaggc tccaaaaccc gaggccgcaa
     5821 aacctgcggc ccccaaaccg gccgcgccac cacccaaacc tccggagccg gcgcccgcca
     5881 aaccgccggc gcccaaacct cctgctcccg agcctctcgc gccgactccc aagccgccgg
     5941 agcttaagcc gtcgccgcct cccgaggcgc aaaagcaacc gccaccggcg ccacaaccag
     6001 ttgcgccgaa gccgccacag ccatctgtgc tcgctgctaa accgccgccc ccaccaatag
     6061 gtccggttgg tacgcctgaa gggcagccgc aaggcctaat gcaggataat tcccgcgccg
     6121 tcgccgtgcc gcagccgtcc gacgatggcg acgatattgt gagctatcaa acgctcgtct
     6181 tcgggatgct ggagttgaag aaggaatttc ccgaggatgc gaaacggcgc ggcgtctttg
     6241 gcacagcgca gatctatttc gaactgaacg acgacggcag cgtgaagagc gagaagttgc
     6301 tgaaatcgag tggcgatgca acgctcgacg tcgaaagcct cgcgctggtt cagcgcgccg
     6361 cgccgttccc caagccgcca actggcgcgc aaaaagtctt tgccgcggac atcacctttc
     6421 cgccgccaaa atagattgca cgcctccgcg catgacttgc ctcgactatt ctagcttcaa
     6481 ttcgccttgc gggggaatcc cggcgacgga gtcgatcgct tgcgcgagca gcgtcaggtc
     6541 ggaggggcgc gagaaacgat ggtcggcatc cttgaccaaa gtcagaacaa ccggatcgcc
     6601 ggcgaggtgt tcgacgagaa gcgacgcatg cgtccacggg acatcgacat ccttcatccc
     6661 ttgcagaata tgcacggggc aatgagtgcg aattgtcccg ccaaggatga gatgcttgcg
     6721 gccttcctcg atcaattcgt gcgtaatcgg ataagtccaa tccgaataag acgaaggcag
     6781 gaagcaacgt cctttcttgt cgatctcctc gcgcaagtcc ggcgggagat gatcccagat
     6841 caa
//
LOCUS       DASWOG010000041         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_74243,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000041 DASWOG010000000
VERSION     DASWOG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74243"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VGB93_00770"
     CDS             complement(<1..132)
                     /locus_tag="VGB93_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0144692.1"
                     /translation="MGGAAEFSGPAKENKMGFKVAVVGATGNLGREMLDILAERRFPA
                     "
     gene            331..1158
                     /locus_tag="VGB93_00775"
     CDS             331..1158
                     /locus_tag="VGB93_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144693.1"
                     /translation="MRATFDDIRPTRLIEQPRRRLRRKRGVSPGLAAVVAILFAAALV
                     LLSGRDPLPSAPGPIAAAPPPDWNEIGDPLQLFDLSAPELVRLPHIYVARRHRLGGRQ
                     DVLTFGQLDGRSPFFRLMLYRVGQEKPEDASLFVDLVRLAATGDVSVIRSSNPAILPT
                     RFGPLETADVDLIASADPVPCLGFRGAGLDGNFRLSGFACGSRTQPISRPALGCLIDR
                     LELDGGGDDSALADYFAETELRRDPSCTGTALGPNRISADWIDENDAPPPLRLRKMR"
     gene            1263..1652
                     /locus_tag="VGB93_00780"
     CDS             1263..1652
                     /locus_tag="VGB93_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144694.1"
                     /translation="MGMRPNLVSILALTASGAVALAGTSGAFASPKHHHHYVHYVHHY
                     APAHVYSSDEPPLTVNKRSWLDPGNVVSQGSEENYVTAGTGFNQTPDEAYFPSRFEED
                     NLPRPLYPTGRTKPLVDFWTPSYPPYP"
BASE COUNT          323 a          553 c          537 g          353 t
ORIGIN      
        1 tgcgggaaac cgcctctcgg cgagtatatc gagcatctct cgcccaagat tgccggtggc
       61 gccgacgacg gcgactttga aacccatttt attctccttc gcggggcccg aaaattccgc
      121 agcgccgccc ataccaaaaa tcgggcgaga aatcacgccc gcatgcatcg atgtttggag
      181 catgttctca tcggaaaagt cgcacaactt ttccggaaca tgctcgcgta aagcctctgt
      241 tgaccctatc ggcaaaaggc tgcgcggcgg taaccggcgg caagtgctgc gatgcgagaa
      301 tcctcctgcc tcatcgaagg cggggaattc atgcgcgcca cattcgacga tataagaccg
      361 acccggctga tcgaacagcc acggcgtcgt ctgcggcgca agcggggcgt gtcgccgggg
      421 ctggccgcgg tcgtggcgat cctctttgcg gcagcattgg ttctgctttc cggccgcgat
      481 ccgctgccca gcgcccccgg cccaattgct gccgcgccgc cgccggactg gaacgagatc
      541 ggcgatccgc tgcaactttt cgatctctcg gctccggagc ttgtccgcct gccgcacatc
      601 tatgtcgcac ggcggcatcg cctcggtggt cggcaggatg ttctgacttt cggccagctt
      661 gatggccgca gcccattctt ccgcctcatg ctttatcgcg tcggccagga aaagccggag
      721 gatgcgtcgc ttttcgtcga tctggtccgg ctcgcggcaa ctggcgatgt gtccgtcatt
      781 cgaagcagca atccggcgat tttaccgacg cgcttcggcc cgcttgagac ggcggacgtc
      841 gatctcatcg cgagcgcaga tccggtgccc tgtctcggtt tccgcggcgc cggattggac
      901 ggcaatttcc ggctgagcgg ctttgcctgc gggagccgga cgcaaccgat ctcccggccg
      961 gcgcttggct gcctcatcga cagactggag ctggacggcg gcggggatga ttcggccttg
     1021 gccgactatt tcgccgaaac cgaattgcgg cgcgacccga gttgcacggg aacggcgctc
     1081 ggccccaacc gcatttcggc agactggatc gatgaaaatg atgcaccgcc gccactccgc
     1141 ttgcgaaaaa tgcgctgaac agccttttga acgggcgcca cgcgctttgg cattcgcgcc
     1201 gaaccaaaac agggctaagt aattgtcgag atagcgtcgc aatgtgcggc aggcggaagg
     1261 gaatgggtat gcgtccgaac ctggtgtcta tactggctct gaccgcctcc ggcgctgttg
     1321 cactggccgg gacgtcgggg gcgttcgcgt cgccgaaaca ccaccatcat tacgtgcatt
     1381 atgtgcatca ttacgcgccg gcgcatgtct attcgagcga cgagccgccg ctgaccgtca
     1441 acaagcgcag ctggcttgat ccgggcaatg tcgtctcgca ggggtctgag gaaaattacg
     1501 ttacggccgg cacgggcttc aaccagacgc cggacgaagc ctatttcccg tcgcgtttcg
     1561 aggaagacaa tcttccacga ccgctttatc cgaccggccg gacgaagcca ttggtggact
     1621 tctggacgcc atcctacccg ccttatccat aagcatttgg aattacgcgt cgaaatcgaa
     1681 atgcgccagg atggggacgt ggtcggatgg ccgttcccag gcgcggcttt cgcgcaagac
     1741 tttcatgccg cataggttcg gggcca
//
LOCUS       DASWOG010000042         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_111004,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000042 DASWOG010000000
VERSION     DASWOG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111004"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VGB93_00785"
     CDS             complement(<1..890)
                     /locus_tag="VGB93_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144695.1"
                     /translation="MYYTDDLSVFIFVVTLAGTFAASFLGRAHARKLNSNALADQKLS
                     KWLVGLSAGATGNSAFIVTGVVGLGYTLGAPALLLPSGWLFGDIAFWTFFPDKINRLG
                     RETRAATLTDVIASGLPATARPHIKLLAGTLILLCLTGYISAQWIAGQKFLEGAFGFS
                     HVLSLVLFAALIVLYTGIGGFRGSVYADTLQALIRIFGTSLALIAVAFVATRHPQAFW
                     QNTAQAGPTFFQLMPHCLVAALVFMLGFAAPAFGFGLGQPQMTSRYLAGRTPGETRGA
                     WWIFIGFIQFTWISMTIFGIA"
     gene            954..1418
                     /locus_tag="VGB93_00790"
     CDS             954..1418
                     /locus_tag="VGB93_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0144696.1"
                     /translation="MSFGFWHSRQDEEPIKVPAIGLEVRVCLPHSASGGELAIIETQN
                     EPGFGPPLHRHSETEVFRVLEGRYLFEVDGRRFHAEAGDVVSVPGRAIHTFVNVADTS
                     SRQLVLFLPGLDAAAFFGELAIATKGGVNKAALDAFGAKWGIEFLGPPLKKK"
BASE COUNT          379 a          454 c          521 g          269 t
ORIGIN      
        1 gcgatgccaa aaattgtcat ggaaatccag gtgaactgga tgaagccaat gaatatccac
       61 cacgcacccc gagtctcgcc cggcgtgcgg ccggcgagat aacgcgaggt catctgcggc
      121 tggccgaggc cgaagccgaa agccggtgcg gcgaagccga gcataaagac aagcgcggcc
      181 acgagacaat gcggcataag ttggaaaaac gtcgggccag cctgcgctgt gttctgccag
      241 aaggcttgag gatgccgcgt tgccacgaag gcgacggcga tgagcgcgag gctggtgccg
      301 aaaatgcgga tcagggcttg caatgtgtcg gcatagaccg agccgcgaaa gccgccgatc
      361 ccggtgtaga gaacgatcaa agccgcgaac aaaacgagcg acagaacatg cgaaaagccg
      421 aaggcgcctt cgagaaattt ttggccggcg atccattgcg ccgaaatata gccggtaagg
      481 caaagaagga tcagcgtgcc agcaagcagt ttgatgtggg gccgcgcggt agcgggcagg
      541 ccgctggcga tcacgtcggt gagcgtggca gcgcgcgtct cgcggccaag gcgattgatc
      601 ttgtcgggga aaaaagtcca gaacgcgatg tcaccgaaga gccagccaga cggcaaaagc
      661 aaagctggcg cgccgagcgt atagcctaaa cccacgacgc cggtaacgat gaaagcgctg
      721 ttgccggttg cgccggcgct gaggccgaca agccatttgc ttagcttctg atcggcgagg
      781 gcgttcgagt tgagtttgcg cgcatgggcg cgcccaagaa aagaggcggc gaaagtcccg
      841 gcgagcgtga caacgaagat gaagaccgat agatcgtcgg tgtaatacat ataaggttcc
      901 gcgcttgcag aaccataaag cgtacatcga cagactgaaa gacggggcga cccatgagtt
      961 tcggcttctg gcattcgcgg caggacgaag agccgatcaa agtcccggcc atcggcctcg
     1021 aagtgcgagt gtgtttgccg cattcagcgt ctggcggcga attggcgatc atcgaaacgc
     1081 aaaatgagcc tggcttcggg cctccgctgc atcgccacag cgaaaccgaa gtctttcggg
     1141 ttttggaagg gcgctattta ttcgaggtcg acggacggcg ttttcatgcg gaagccggag
     1201 acgttgtcag cgtgccgggc cgggcaattc acacgttcgt caatgtcgcc gatacgtcga
     1261 gccgccagct ggttttgttt ttgccgggcc ttgatgccgc ggcattcttc ggcgaactgg
     1321 caatcgcgac gaagggcggt gtgaacaagg cggcgcttga tgctttcggc gcaaagtggg
     1381 gcatcgaatt cctcgggccg ccgttgaaaa agaaatagct cgataaagaa atgccggcag
     1441 gaatcccgcc ggcatgaatc ttcaagcaaa taaagcgcgt tgattaccga cgatgaccac
     1501 cgccgcggtg accgccaccg ccgcgatgtc cgccgccatg cccaccgccg cgatgtccgc
     1561 caccacgatg accaccaccg tggtgtccgc caccccggtg tccgccgccg tgccagccac
     1621 cat
//
LOCUS       DASWOG010000043         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_294339,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000043 DASWOG010000000
VERSION     DASWOG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294339"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VGB93_00795"
     CDS             <1..770
                     /locus_tag="VGB93_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0144697.1"
                     /translation="TAGGIASLGPYGQFLLSFETLGVAINAGYRKFSSLLQAATEMDL
                     IVRNGWAHWSYRITAPIVVGRQRNEILAIGYMLNLLRHFLGSRWSPDAILVPGQLQGR
                     GEIETIFSGDVLRGERATVVFRAELLDAPNPSRYRHPEMFEEVPCGDAFVAIAEHMIR
                     LAMLEKAPGIDQVAERLKLSRRTLQRRLAAHGVTFERLAEGVVFAQARAKLDAKQQAI
                     TDIALELGYSDPAHFSRAFRRWTGETPRHWRSRQLQS"
     gene            complement(767..1609)
                     /locus_tag="VGB93_00800"
     CDS             complement(767..1609)
                     /locus_tag="VGB93_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0144698.1"
                     /translation="MKLIRRPFALISLLLAAAGLAACENGNSLGPAGAGVAEVTQETP
                     QQSQANISSLSDVIQRNPSSAEAYNTRGVAYARIAQYDAAIADFSQAIKLNPNDAAAL
                     TNRALAYRQTNHDDLALADFNQAIIANPNHAPAYIGRANLLRVNDKLDQARADLDNAI
                     RLNPENAQAYHARGLIFQRQGNYQAAITDFDNAIDRDPFVGAPYEARGESLLALGKAD
                     KAIEDFNAALNVNNKDANAWAGLGLAYEKLGNRNKAAESYDRALAFDPNNKVARDGEA
                     RLRA"
     gene            complement(1794..2033)
                     /gene="rpsU"
                     /locus_tag="VGB93_00805"
     CDS             complement(1794..2033)
                     /gene="rpsU"
                     /locus_tag="VGB93_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0144699.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     REKAEAVRRARKLARKKLQREGLLPMKPKPVVGAR"
BASE COUNT          380 a          615 c          655 g          456 t
ORIGIN      
        1 cgaccgcggg cggcattgct agtctcgggc cttacggcca attcctcttg tcgttcgaaa
       61 cgctcggtgt agccatcaat gccggctatc ggaaattttc cagcctgctt caagccgcga
      121 ctgagatgga tctcatcgtg cgtaatggct gggcgcattg gagctaccgc atcactgcgc
      181 cgatcgttgt cgggcggcag agaaacgaga ttcttgccat cggctatatg ctcaatctgc
      241 ttcgtcattt tctcggatcg cgctggtcgc cggacgcgat cctcgttccg ggccagttgc
      301 aagggcgtgg cgagatcgag acgattttta gcggcgacgt cttgcgcggc gagcgcgcga
      361 cggttgtctt ccgcgctgaa cttcttgatg cgcccaatcc ctcacggtac cggcaccctg
      421 agatgttcga ggaagtgccg tgcggtgatg cgtttgtcgc aatcgctgaa catatgatcc
      481 ggctcgcgat gctggagaag gcaccgggca tagatcaggt ggctgaacgg ctgaaactct
      541 cgcggcgcac gcttcagcgc cggcttgcgg cgcatggcgt gacattcgag cgattggcag
      601 agggcgttgt cttcgcgcag gcgcgcgcca agcttgatgc caagcagcag gccatcaccg
      661 acatcgcatt ggaattgggc tattccgatc cggcgcattt cagccgcgcg tttcgccgct
      721 ggaccggtga aacgccgagg cattggcgat cgcgtcagtt acagagctaa gcccgcaggc
      781 gcgcttcgcc gtcgcgcgcg actttgttat tgggatcgaa cgcgagcgcg cgatcgtagg
      841 attcagccgc tttattgcga ttgccgagct tctcataggc gaggccgaga ccggcccagg
      901 cattggcgtc cttgttgttg acgttcagtg ccgcgttgaa gtcttcgatc gccttgtccg
      961 ccttgccgag cgcgagcaag ctttcgccgc gcgcttcata aggcgcgccg acgaaaggat
     1021 cacgatcgat cgcgttgtcg aaatcggtga tcgccgcctg ataattgccc tggcgttgga
     1081 agatcagacc gcgcgcatga taggcctgtg cattctccgg attaagccgg atggcgttgt
     1141 cgagatcggc gcgcgcctga tcgagcttat cgttcacgcg cagcagattg gcgcggccga
     1201 tataggcagg ggcgtggttg ggattggcga tgatcgcctg attgaagtcg gcgagcgcga
     1261 gatcgtcgtg attggtctgg cgataggcga gcgcgcggtt ggtcagcgcg gcggcgtcgt
     1321 tgggattgag cttgatcgcc tggctgaagt cagcgatcgc ggcatcatat tgcgcgatgc
     1381 gcgcataggc gacgccacgc gtattgtaag cttcggcact actgggattg cgctggataa
     1441 cgtcggacag cgagctgata ttggcctgag actgttgcgg cgtctcctgc gtcacctcgg
     1501 cgacgcccgc gcccgctgga ccgagcgaat tcccgttctc gcacgccgcg agaccggccg
     1561 ccgcgagcaa aagcgaaatg agcgcgaaag gacgtcgaat caatttcatc gaaattcaag
     1621 tcccggatca actcgttctt gctttgcgcg tgaatcgcca gaaacggtac gatcagacag
     1681 gccgcggggc ctttaggccc cggcaatccg gcaagattgc gaccaatccc gccggctcat
     1741 cggataatag gctttgatat cgaaaacgat gcgccgtcag tgcgcaatcg aacttagcgc
     1801 gcgccgacga ccggcttcgg cttcatcggc aacaggcctt cacgctgcag cttcttccgc
     1861 gccaatttgc gcgcccggcg caccgcttcg gccttttcac gcgcccgttt ctcggagggc
     1921 ttctcgtaat ggccgcgaag cttcatttcg cggaaaatcc cttcgcgctg catcttcttc
     1981 ttcagcgcct taagggcctg gtcaacattg ttgtcacgaa ccagaacttg cacgcggcga
     2041 tccttgtctt gcgccgacgg ctcatcgccc cggcaggatg aaagtctaga ctttcgggaa
     2101 tagaat
//
LOCUS       DASWOG010000044         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_239265,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000044 DASWOG010000000
VERSION     DASWOG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239265"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VGB93_00810"
     CDS             complement(<1..965)
                     /locus_tag="VGB93_00810"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEY0144700.1"
                     /translation="MLLWLLGLAVIAVLIWSGVWLFAAQQTGTALQAWVAREKAFGRV
                     WSCPNPKIGGYPAAISINCASPRFDGLIFGHTYGGTLQGMHVTASVSHPRDIDIAADS
                     PFVATSKDQKVNFQLTWGDLDVRLSGLPQDLWQAVVSGEDIAVHGSAGAVGFTGAVHE
                     TAVTAAQRVTQDGPAIDFDLTLGAATLPLIEQVLGPGAPTDIAAQGMVTKTSFETRLT
                     PAQNLDLWRTAGGHVDLTSFTLTRGDTKFAAKGTFALSAAHRVNGHFETTSSGLEPVL
                     QHYGIDPSLLSFGALLGNLLSGHSNDAQAQGPTKLHLPIELDDGRL"
     gene            1141..1674
                     /locus_tag="VGB93_00815"
     CDS             1141..1674
                     /locus_tag="VGB93_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY0144701.1"
                     /translation="MMEDFWVFGYGSLMWRPGFAFSEREVARVRGYHRALCVYSHVHR
                     GTPERPGLVLGLDRGGSCRGIVFRVMAEHRDETIAYLRAREQVTSVYKEAYLNAQLDD
                     GRCVMALSYIVDRSHVQYAGDLAREDVARFVAQGQGVSGANPDYVRATQAHLAELGIA
                     DPILDWLAAQFADEAAR"
     gene            complement(1671..2207)
                     /locus_tag="VGB93_00820"
     CDS             complement(1671..2207)
                     /locus_tag="VGB93_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144702.1"
                     /translation="MTMTQRPQTGLTLKSLSGRGDSARAMSLLFLLRRWREKNATMVV
                     RPPADDASNASLASLAGGDLATRFHSWRGRSGRRYICSVFRVEHHTEDAGLPDFAAAV
                     VIAVKTDVVGNRQVVGFCQCEHGFGPVARAAFVTTAIAAGAQQWHVHLLTADPQKRRA
                     AIEDIEALCHPSRELLPA"
     gene            complement(2301..3068)
                     /locus_tag="VGB93_00825"
     CDS             complement(2301..3068)
                     /locus_tag="VGB93_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0144703.1"
                     /translation="MRLLRSTLFNVCFYVLSIILMLAALPALARGGDAVESHARRWAR
                     STIVLLDHICGVNVEFRGMENLPAGPVIVAAKHQSTLDVFVLLLAVTRFTFIYKRELD
                     FLPLFGAYLRGCGQISIDRSKRVGVLSQIVTAAKAKFAENRRLIIFPEGTRRPVGAPP
                     VYKGGVARIVEATGVVCVPVALNSGLFWPRHRFSRPPGGMVVEFLPPVPPGFDKDNFM
                     RILQTQIETATDRLVAEAVDRDAGLAPSAPEAGLSRS"
     gene            complement(3081..>4238)
                     /locus_tag="VGB93_00830"
     CDS             complement(3081..>4238)
                     /locus_tag="VGB93_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0144704.1"
                     /translation="EMLAAGLNSICGGRDHIAIDVEREITLWLGEAFDFPRDSSGVFV
                     TGSSMANMIATLVARTDVLGEQVRTDGLRATEQLVGYASTEAHACIERAFEMSGVGSA
                     FLRRIPVNEKHEMDGVALRAAIAEDRGAGLLPFIVVGSAGTVNTGAIDDLTRLADICA
                     EEDVWFHVDGAFGALGVLSPELKPLLNGLERADSVALDFHKWLQVPYDAGFFICRHPD
                     LHRRTFMSTAAYLSRAPRGLAAADIWPCDLGPDLSRGFRALKTWFTFQSFGTEQLGAI
                     LAQTCRVARHLEVLIQRSTIFELSAVVTLNIVCFSVKGDTTGGINREIVMDLHESGVA
                     APSTTKIGGRPVIRAAIVNHRTQMRDMETFVAAAEACAARVMAERAAPVAP"
BASE COUNT          857 a         1312 c         1333 g          736 t
ORIGIN      
        1 agacgtccgt catcgagttc aatgggcaga tgcagcttcg tcggcccttg agcctgcgcg
       61 tcgttcgaat ggccgctgag caaattacca agcagcgcgc cgaaactgag cagactcgga
      121 tcaatgccat aatgctgcaa caccggctca agcccgctgc ttgtcgtctc gaagtggcca
      181 ttcacccgat gcgcggcgct gagcgcgaac gtgcccttcg ccgcaaattt ggtatcgccg
      241 cgcgtaagcg tgaaagacgt aagatcgaca tgaccgccgg cggtccgcca gagatcaagg
      301 ttctgcgccg gtgtcaggcg cgtctcaaaa cttgtcttgg tgaccatgcc ttgcgctgca
      361 atgtcggtgg gggcgccggg accgagaacc tgttcgatga gcggcagcgt cgccgcgccg
      421 agggtcaaat cgaaatcgat tgcggggcca tcctgcgtaa cgcgctgcgc cgccgtcacc
      481 gcagtttcat gcacggcgcc cgtaaacccg acagcgccgg cgctgccatg caccgctata
      541 tcctcgccgc tgacgaccgc ctgccaaaga tcttgcggca acccgctgag gcgaacgtcg
      601 agatcgcccc acgtcagttg gaaattgacc ttctgatctt tggaggtcgc gacgaagggc
      661 gagtcggccg caatgtcgat gtcgcgcgga tgcgaaacgc tcgccgtcac gtgcatccct
      721 tgcaatgttc cgccgtaggt gtggccgaag atcagaccgt cgaagcgcgg cgacgcacaa
      781 ttgatggaaa tcgccgcggg atagccgccg atcttgggat tgggacatga ccagacgcga
      841 ccgaacgcct tttcccgcgc gacccaagcc tggagcgccg tcccggtctg ctgcgcggcg
      901 aacagccaga cgcccgacca gatcagcacg gcgatgacgg ccaatcccag gagccaaagc
      961 agcaagcttg gccgcttgcg ctgcaagcgc ggagtcccaa cgctcggtcc gttctccaat
     1021 aatcttctcc actcgggttc cgggatgcgc tgtgtaaccc aaaacagaaa tccggaggaa
     1081 ttttgttggg gccgcccgcg ctggcgttac agcgcagcgt atatggctta agtttcacct
     1141 atgatggaag atttctgggt gttcggctac ggctcgctga tgtggcggcc gggctttgcc
     1201 ttctcggagc gcgaggtcgc acgggtacgc ggctatcatc gcgcgctttg cgtctattcg
     1261 catgtccatc gcggcacgcc ggagcggccg gggcttgtgc ttggccttga tcgtggcggc
     1321 tcatgccgtg gcatcgtctt tcgggtgatg gcagagcatc gcgatgagac gatcgcctat
     1381 ctgcgcgccc gcgaacaggt cacgtccgtc tataaggaag cttatctcaa cgcgcagctc
     1441 gatgatggcc ggtgcgtgat ggctttgtct tacattgttg accggagcca cgttcaatat
     1501 gccggcgacc tcgcgcgcga ggatgtggcg cggtttgtgg cgcaaggcca gggcgtttcc
     1561 ggtgccaatc cagattacgt cagggcgacg caggcgcacc tcgccgaact cggcatcgcc
     1621 gatccgatcc tcgattggct tgctgcccaa tttgcggatg aagcagcgcg ctaggctggc
     1681 aagagctcgc gcgaagggtg gcacagcgcc tcgatatcct cgatcgcggc gcggcgtttt
     1741 tgcggatcgg cagtgagaag atgcacatgc cattgctggg caccggcggc gattgcagta
     1801 gtcacgaagg cggcgcgcgc gaccggaccg aagccatgtt cgcattggca gaacccgaca
     1861 acctgacggt tgccgacaac atccgtcttt accgcgatca cgaccgcggc ggcgaaatcc
     1921 ggcaggccgg catcctcagt atgatgctcg acgcggaaca ccgaacagat gtaacgccgt
     1981 ccggaacggc cgcgccatga atgaaatcgt gtcgccaagt cgccaccggc gaggcttgcg
     2041 agcgaagcgt tgctcgcgtc gtcggccggc gggcgcacta ccatggtggc gttcttctcg
     2101 cgccagcgcc gcagcaaaaa gagcaacgac atggcgcggg cggaatcgcc gcggccgctc
     2161 aaactcttca acgtcaagcc ggtctgcggc cgttgcgtca tcgtcataat caaacccctg
     2221 tacgctacag agtgattcgc taatgagaac aaagttggaa caaaagggca ggcgggtcaa
     2281 gtctgacggc cgaggctaag tcagctccgg gaaagcccag cttccggtgc ggaaggcgcg
     2341 aggcccgcgt ctctatcgac agcttctgcg accagccgat cggtcgcggt ctcgatctgc
     2401 gtctgcaaaa tccgcatgaa attgtctttg tcgaaccccg gcggaaccgg cggcaaaaat
     2461 tccaccacca tcccgcctgg cgggcgactg aaacgatggc gcggccagaa aagcccggaa
     2521 ttgagcgcca ccgggacgca gaccacgccc gtcgcttcga cgatccgcgc gacgccgccc
     2581 ttataaaccg ggggcgcccc gacaggacgg cgcgtccctt ccggaaagat gatgaggcgg
     2641 cggttttcgg cgaatttggc tttcgccgcg gtgacgatct gcgagagaac gccgacacgc
     2701 ttgctgcggt cgattgaaat ctgtccgcaa ccgcgcagat aggcgccaaa gagcggaaga
     2761 aagtcgagtt cgcgtttata gatgaaggtg aagcgcgtca cggcgaggag cagcacgaac
     2821 acatcgagcg tcgattgatg cttggcggcg acgatcaccg ggccagccgg caaattttcc
     2881 atcccgcgaa attcgacgtt gacgccacaa atatgatcga gcagcacgat tgtcgatcgc
     2941 gcccagcggc gcgcatggct ctcgacggcg tcaccgccgc gtgccaaggc tggcagtgcc
     3001 gcgagcatca aaatgatgct cagtacataa aagcagacgt tgaagagagt cgatcgcaat
     3061 aatcgcatgg tcgctcgctt ttaaggcgca accggcgccg cgcgctcggc catgacgcgc
     3121 gccgcacagg cttcagccgc cgcgacgaac gtctccatat cgcgcatttg cgtccggtga
     3181 ttgacgatcg cggcgcggat caccggccgg ccgccgattt ttgtggtcga aggtgcggcg
     3241 acgccggatt catgcaggtc catgacgatc tcgcggttga tcccgcccgt cgtgtcaccc
     3301 ttcacgctga agcagacgat gttgagcgtc acgacggcgc tgagttcgaa gatcgtcgag
     3361 cgttggatca gcacttcgag atggcgcgcg acccggcagg tttgcgccag aatcgcgcca
     3421 agctgctcgg tgccgaagga ctggaacgta aaccacgtct tcagcgcacg aaagccgcgc
     3481 gacagatcgg gcccgagatc gcacggccag atatcggccg ccgcgaggcc gcgcggcgcg
     3541 cgcgacagat aggccgcggt gctcatgaac gtccggcgat gcagatcggg gtgacggcag
     3601 atgaagaagc cggcgtcgta gggcacctgc agccatttgt ggaagtcgag cgcgacggaa
     3661 tcggcgcgtt caagtccatt cagcaaaggc ttcagttcag gcgacaacac gcccagcgcg
     3721 ccaaacgcgc catcgacatg aaaccagacg tcttcctcgg cgcagatatc ggctagccta
     3781 gtcagatcgt cgatggcgcc cgtattcacc gtgccggcgg agccgacgac aataaacggc
     3841 agcaagcccg cgccgcgatc ctccgcgatg gcagcacgca atgccacgcc atccatttcg
     3901 tgtttttcat tgaccggaat gcgccgcagg aaggccgagc cgacaccgga catttcaaag
     3961 gcgcgctcga tacaggcgtg cgcctcggtc gaagcgtaac cgacaagctg ctcggtcgcg
     4021 cgcaacccgt cggtgcggac ctgctcgccg agaacatcgg tgcgcgcgac gagcgtggcg
     4081 atcatattcg ccatcgacga accggtgacg aaaacgccgg aggagtcgcg cggaaagtcg
     4141 aacgcctcgc caagccagag ggtgatctcg cgttccacat cgatcgcgat atggtcgcgg
     4201 ccgccgcaaa tggaattaag ccccgcggcg agcatttc
//
LOCUS       DASWOG010000045         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_37661,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000045 DASWOG010000000
VERSION     DASWOG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37661"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGB93_00835"
     CDS             complement(<1..520)
                     /locus_tag="VGB93_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0144705.1"
                     /translation="MSLLISTLGRYLTWRFFKTIAAVFLAIVGTIYVFDFVELLRRAN
                     SIQGVTSGYIAYLTLLRTPAVAEQILPFCVLFGTMAAFIDLTRKLELIVARSAGVSVW
                     QFLLPPVAIALLIGIFSVIAFNPLSALMKQRADVIEAQLFGRAGTVQGDGTLWIRQSG
                     IDGEAILRAKSAL"
     gene            1109..2707
                     /locus_tag="VGB93_00840"
     CDS             1109..2707
                     /locus_tag="VGB93_00840"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0144706.1"
                     /translation="MSHAAKIEFVPLTSVSAAGAAKANGKAKGVATLVIFAGEDLNLG
                     KPTRALLGGDGEALVKKAGGAAKFKGKNASALDILAPAGLAADRLLVIGVGASEETKK
                     AKGKEATKAKPRDFLGLGGYVLGKIGDAPNATIMFDLPHNPQDAAVAAAEFAEGAALR
                     NYKFDLYKTKKKDDESTEPVALAIAVEDPAAAKREAKGRTAVAEGVIIARSLVNEPAN
                     VLYPEEFAKRAKELEKLGVEVDILDKAAMTKIGMGALLGVGQGSGRESRVVIMRWRGD
                     KGKEGKSKPIAFVGKGVTFDTGGISIKGAAGMEDMKGDMAGAACVVGLMHALASRKAK
                     VDAIGAIGIVENMPGPDAQRPGDIVKSLSGQTIEVINTDAEGRLVLADVLWYVQDKYK
                     PLFMVDLATLTGAVMVALGNEYAGLFSNDDTLSERLTEAGKETGEKLWRLPLGPSYDK
                     LIDSKFADMKNTGGRHGGSITAAQFIQRFVNGTPWAHLDIAGTGMNAPANDINQSWGS
                     GFGVRLLERLVSDYYEGRAHSQKD"
     gene            2707..3165
                     /locus_tag="VGB93_00845"
     CDS             2707..3165
                     /locus_tag="VGB93_00845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY0144707.1"
                     /translation="MEVWFYHLQSQRLEQTLPALLERALANGWRTVVQASSEERLDII
                     DEVLWTYADASFLAHGRARDGDADMQPVYLTCAAENPNGARLRLFIDGADAAAHLAAD
                     AAYERAILLFDGNDSDQVGAARAQWKILKEQGVPITYWQQNERGGWEKKA"
BASE COUNT          703 a          938 c         1034 g          587 t
ORIGIN      
        1 caagcgcgct tttggcgcgc aaaatcgcct cgccatcgat gccgctctgg cgaatccaga
       61 gcgtgccgtc gccctgaacc gtaccggcgc ggccaaaaag ctgggcttcg atgacgtcgg
      121 cgcgttgttt catgagcgcc gacagcggat tgaaggcgat gaccgagaaa atcccgatga
      181 gaagggcgat ggcgacgggc ggcagcagga attgccacac cgaaacaccg gcggagcggg
      241 ccacaatcag ttccagctta cgggtgagat cgatgaaggc cgccatcgtc ccgaacaaaa
      301 cgcagaacgg caaaatttgc tcggcgaccg ctggggtgcg gagcagggta agataggcga
      361 tgtagcccga ggtcacgccc tgtatcgaat tggcacggcg cagcagttcg acgaagtcga
      421 aaacatagat cgtcccgacg atcgccagga agacagccgc aattgtcttg aaaaaccgcc
      481 aggtgaggta acgcccgagc gtcgagatga ggaggctcat gactcggtcc ggcgtgtcga
      541 tgcgagggcg gagaataagt gtcgcgaccg cgccggcgca ggaatacgcc cagcagatgt
      601 ttttgcagag ctggtcaatc tggcgacgaa caacatacgc gggcggttcc gatgaagaaa
      661 aaacgttgtc atccaggcag atccggggct gctaaagtga catacgtcag acacgcggcc
      721 gggtggccat acctttgcgt gaacgtctct ccgtcgggct attggaatct gtttcgcgca
      781 agccgaccct cccggcccat cggaatcgcg aataggtccc tgaaatccta ttccaatttg
      841 gctaactagt cggcttcatt ggtgcaagtc gctcggggct ttttcagggc ggtccgcggt
      901 cgatggcgag cgcccgttgc gccgcggcca cggccatccg taccgtactt tccgcgaaaa
      961 aactggcgag gaaacgccaa ccggtgctag ggcttgcggt catagcaagg cttcatcacg
     1021 ccgcccggct cgggaaaagc gactaagcgt ctgttttatc gcgcgatctt ccaaagccgc
     1081 caaaagatac caaggggata acgagcccat gtcgcacgcc gccaaaatcg aatttgttcc
     1141 gctcacatcc gtgtcagccg caggcgcggc gaaagcgaac ggaaaagcca aaggcgtggc
     1201 gacgctggtc atcttcgctg gcgaagattt gaacttgggt aagcccaccc gcgctctcct
     1261 cggtggcgat ggtgaggcgc tggtcaaaaa ggctgggggc gccgcgaaat tcaagggcaa
     1321 gaacgcctcg gcgctcgata ttctcgcgcc cgcaggcctg gcggctgacc ggttgctggt
     1381 catcggcgtc ggcgcaagcg aagaaaccaa gaaagccaag ggcaaggaag cgaccaaggc
     1441 caagccgcgg gattttctcg gcctcggcgg atatgtcctt ggcaagatcg gcgatgcgcc
     1501 gaatgcgaca atcatgttcg atcttcccca caatccgcag gatgccgcgg tcgcggcagc
     1561 cgaattcgcc gaaggcgccg cgctgcgcaa ttacaaattc gatctctaca agaccaagaa
     1621 aaaggatgac gaatccacag agcccgtcgc gctcgcgatt gccgttgaag atcccgccgc
     1681 ggccaaacgt gaggccaagg ggcgtaccgc ggttgcggaa ggcgtgatca tcgcccgcag
     1741 tctcgtcaac gagccggcca acgtgcttta tcccgaggaa ttcgccaagc gcgcgaagga
     1801 actcgaaaag ctaggcgtcg aagtcgacat tctcgataaa gccgcgatga ccaaaatcgg
     1861 catgggcgcg ctgctgggag tcgggcaggg ctcgggacgt gaaagccgtg tcgtcatcat
     1921 gcgctggcgc ggcgacaagg gcaaagaggg caagtcgaag cctatcgctt tcgttggcaa
     1981 gggcgtgacc ttcgatactg gcggcatttc catcaagggt gccgccggca tggaggacat
     2041 gaagggcgac atggccggcg ccgcctgcgt cgtcggcctc atgcacgcgc ttgcttcgcg
     2101 caaggcgaag gttgatgcga tcggcgccat cggcatcgtc gagaacatgc cggggcctga
     2161 tgcgcagcgc cccggcgaca tcgtcaaatc gctatccggc cagacgatcg aagtgatcaa
     2221 caccgatgcc gaaggccgcc tcgtgctcgc cgacgttctt tggtacgtgc aggacaagta
     2281 caagccgctc ttcatggtcg atctcgccac gctgaccggc gcggtgatgg tcgcgctcgg
     2341 caatgaatac gcgggtcttt tctcgaacga tgacacgttg agcgagcgat tgactgaagc
     2401 ggggaaggaa accggcgaga agctttggcg tctgccgctt gggccttcct acgacaaact
     2461 catcgattcc aaattcgccg atatgaaaaa taccggcggt cgccatggcg gatcgatcac
     2521 cgcggcgcaa tttatccagc gtttcgtcaa cggcacgccc tgggcgcatc tcgacattgc
     2581 cggcaccggc atgaatgcac cggcgaacga catcaaccag agctggggtt cggggttcgg
     2641 cgtgcggctg ctcgaacgtc tcgtgagcga ctattacgag ggccgcgccc attcgcagaa
     2701 ggattgatgg aagtctggtt ctaccatctg caaagtcagc ggcttgaaca gacgttgccg
     2761 gccttgctcg aacgtgcgct cgccaacggc tggcgcacgg tggttcaggc atcgagcgag
     2821 gaacggctcg acatcattga tgaggttctc tggacctatg ccgatgcgag ttttctggca
     2881 cacggccgcg cgcgcgacgg cgatgccgac atgcaacccg tctatctgac ctgtgctgct
     2941 gaaaatccga acggggcgcg tttgcggctg ttcatcgatg gcgccgatgc cgccgcgcat
     3001 cttgccgccg atgccgctta cgagcgtgcg atccttttgt tcgatggcaa cgattcagac
     3061 caggttggcg ctgcccgcgc acaatggaag attctgaaag agcagggtgt tccgatcacc
     3121 tattggcagc agaatgaacg cggcggctgg gagaagaagg cgtagcgcct cgattggcac
     3181 taggggggat gatgagcgtg cgagcctatc ttctgttggc gtttcttgca ctttcatgcg
     3241 cgccggttcg agcggacgac tg
//
LOCUS       DASWOG010000046         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_275966,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000046 DASWOG010000000
VERSION     DASWOG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275966"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1081
                     /gene="gap"
                     /locus_tag="VGB93_00850"
     CDS             74..1081
                     /gene="gap"
                     /locus_tag="VGB93_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024279732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0144708.1"
                     /translation="MAVNVAINGFGRIGRNVLRAIAESGRRDINVVAINDLGPVETNA
                     HLLRFDSVHGRFPGEVKVDGDTIDIGRGPIKVTAVRDPAELPHSALGVDIVLECSGVF
                     TSRAKASAHLRAGAKRVLISAPADEADLTVVYGVNHDKLLPEHRVVSNASCTTNCLAP
                     VAKVLNDAVGIEHGFMTTIHSYTGDQPTLDTLHKDLYRSRAASLSMIPTTTGAAKAVG
                     LVLPELKGKLDGSSIRVPTPNVSMIDFKFVAKRTTTPDEITDAIKRAAEQQLKGILSF
                     TDSPNVSIDFNHDPHSSVFHVDQTRVIDGTFVRVVSWYDNEWGFSNRMADTAVAMGKL
                     G"
     gene            1078..2301
                     /locus_tag="VGB93_00855"
     CDS             1078..2301
                     /locus_tag="VGB93_00855"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0144709.1"
                     /translation="MTRTAPLSPFPLLDDAELAGKRVLIRVDLNVPMDGDKVADATRI
                     DRILPNLQEISAKGAKVIILSHLGRPKGREEKYSLRPVIAELSTRLGKPVAFAADCVG
                     PEAEKAVEALKNGEYLLLENTRFHAGEEKNDEAFLDRLAALGDVYVNDAFSCAHRAHA
                     STEGLARRLPSFAGRSMQVELEILTNLLSHPVRPVMAIVGGAKVSTKLELLGNLMCRV
                     DILVIGGGMANTFLASQGKNVGKSICETELANVARKIMADAAAENCEIVLPTDVVVAA
                     KFAAGAPSRVVPVDEVGDEDMILDIGPTSAARVETLIEKARTLVWNGPFGAFEISPFD
                     AGTNAIAKVAARFTKSGQLESIAGGGDTIAALNKSDAAKDFTYISTAGGAFLEWLEGK
                     ELPGVEALRVNSRRR"
     gene            2344..3366
                     /locus_tag="VGB93_00860"
     CDS             2344..3366
                     /locus_tag="VGB93_00860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY0144710.1"
                     /translation="MNEQQLAQTAQALVAPGRGILAADESSSTIAKRFSAIGVESTAD
                     SRRDYRELLFRSTQAMQKYISGVILYDETIRQKALDGTPLVTLIERVGSLPGIKVDAG
                     AKPLPGFPSETITEGLDGLAGRLKDYYDLGARFAKWRAVIDIADGIPTRGAILANAEA
                     LARYAALCQEANIVPIVEPEVLMDGNHSLQRCYEVTETVLRAVFAALYEQRIFLEGMI
                     LKPNMVVPGMKSGHRASPDEVAAATIRLFKNSVPVAVPGIAFLSGGQSDFDATANLDA
                     LVRAAPPWPMTFSYGRALQAAPQKAWSGKPANVGAAQTAFTHRAYMNSLAALGQWSES
                     AEKAAA"
     gene            3501..4121
                     /locus_tag="VGB93_00865"
     CDS             3501..4121
                     /locus_tag="VGB93_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0144711.1"
                     /translation="MSEDAPRLYLITPPLVDAVAFSVTFEAALAAADVACVLLRFDPR
                     AHAGAVAKKLLALAQPRGVACLVGDPQLAGAIEADGVHVSAPGDALDAALKAMKPNHI
                     VGVGGLVSRDDAMAAGESGADYLMFGGPDTPQAFGEIRERIAWWAEIFNLPCVAYAHQ
                     IAEVAGFVEMAADFIALETAVWDDPRGPGAAVADVNRLLARGRTGA"
     gene            4118..5104
                     /locus_tag="VGB93_00870"
     CDS             4118..5104
                     /locus_tag="VGB93_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0144712.1"
                     /translation="MNRLTLSVCCMLLAFSAEPVYAAQPAAAPAALPAIAKPAPALPQ
                     PNPNANLNLDLAYGAYQRGYYITALGEARKRLAANPNDGPAMTLIGQLFDQGLGVVTN
                     AREAAAWYKAAAEHGDREGIFLYGLAKLTGNGVAQDRDGAAALFTKAAAMDDPGALYN
                     LGVLALETPKRAPDFPKAADYFRRAAVLGSPDAAYSLALFYREGKGAPQDDVMAAEWM
                     KQAADEGQVSAEVEYAIMLFNGVGVAKDEAAAAKLFLKAAARNNVVAQDRAARLLALG
                     RGIQKDVVEAMKWHLLASAAGEKDAWLDGILNSLTPEQKIEVEVAVKRALAR"
BASE COUNT          979 a         1534 c         1627 g         1026 t
ORIGIN      
        1 gcggcgctca tctgcataac aagatggcgc caacctaggc ctgcgcttta tgcgccaaaa
       61 ggagcggacg tttatggcgg tgaatgttgc aatcaacggt ttcggacgca tcggccgcaa
      121 tgttctgcgg gccattgccg agtccggacg ccgggatatt aacgtcgtcg ccatcaatga
      181 tctgggtcct gtcgaaacca atgcccacct tttgcgtttc gactctgtgc atgggcgttt
      241 cccgggcgag gtgaaagtcg atggcgacac catcgacatt ggccgcggtc cgatcaaggt
      301 gacggccgtg cgcgacccgg ctgaactccc gcatagcgcg ctcggcgtcg acatcgtgct
      361 cgaatgttcc ggcgtgttca cgtcaagggc gaaagcctca gcgcacttaa gggcgggtgc
      421 gaagcgcgtt ctcatttcgg cgccagccga cgaagcggat ttgacagtcg tgtatggcgt
      481 aaatcacgat aagcttttgc ccgagcatcg cgtcgtttcg aacgcctcct gcaccacaaa
      541 ctgtttggcg ccggtcgcca aggtgctcaa cgacgccgtt ggcatcgagc acggcttcat
      601 gacgacgatc cattcctaca ccggcgatca gccgacgctc gacacgctgc acaaggatct
      661 ctaccggtcg cgtgcggcat ctctttcgat gattccgacg acgaccggcg cagcgaaagc
      721 cgtcggcctc gtcctgcctg agctcaaggg caagctcgat ggctcgtcaa ttcgcgtacc
      781 gacgccgaat gtgtcgatga tcgatttcaa attcgtcgct aagcgcacga cgacgcccga
      841 cgaaatcacc gatgcgatca agcgtgcggc ggaacagcag ctcaagggca ttctcagctt
      901 cacggacagc ccaaacgttt cgatcgattt caatcacgat ccgcactcat ccgttttcca
      961 tgtcgaccag acccgggtca tcgacggcac gtttgtacgt gtcgtctcct ggtatgataa
     1021 cgaatggggt ttttccaacc ggatggcgga tacggcagtg gcgatgggga aactgggatg
     1081 acacggactg caccactttc accttttccg cttttggacg atgccgagct ggccggaaaa
     1141 agggtcctga tccgcgttga cctcaacgtg ccgatggatg gcgacaaggt cgcagacgcg
     1201 acccgcatcg atcgcattct gcccaatctg caggaaatct cggcgaaagg ggcgaaggtc
     1261 atcatccttt cgcatctcgg tcgcccaaaa ggccgggaag aaaaatattc gctgcgcccg
     1321 gttatcgcag aactgtcgac gaggctcggc aagccggtcg cctttgccgc ggattgtgtg
     1381 ggtcctgaag ccgagaaggc cgtcgaagct ctgaagaacg gcgagtatct cttgctcgaa
     1441 aacacgcgct ttcatgccgg cgaggaaaaa aacgacgaag ccttcctcga caggctcgcg
     1501 gcgctcggtg atgtttacgt caacgacgcg ttctcctgcg cgcaccgcgc ccatgcctcc
     1561 acggaaggtt tggcgcgccg gctgccgtcc tttgcgggac ggtcgatgca ggtcgaactt
     1621 gaaatcctca ccaaccttct ttcgcatccg gtgcgccccg tcatggcgat tgtcggcggc
     1681 gcaaaagtat cgaccaagct cgaattgctc ggcaatctga tgtgccgcgt cgatattctc
     1741 gtcatcggcg gcggcatggc caacactttc cttgcctcgc aaggcaagaa cgtcggcaag
     1801 tcaatctgcg agacagagct cgccaatgtc gcgcgcaaaa tcatggcaga cgcggcggcg
     1861 gagaattgcg agatcgtgct gccgaccgat gtggttgttg cagcaaaatt tgcggccggg
     1921 gcgccatcgc gcgtcgttcc ggtcgatgag gtcggcgacg aggacatgat tctcgatatc
     1981 gggccgacgt ctgcggcgcg tgtcgagacg ctgatcgaga aagcccgcac gctcgtctgg
     2041 aatggtcctt ttggggcttt cgagatttcg ccgttcgatg ccggcacgaa tgccatcgcg
     2101 aaggttgcgg cgcgtttcac caagtccggc cagctcgaaa gcatcgccgg gggtggcgat
     2161 acgatcgcgg cgctgaacaa gtcggacgcg gccaaggatt tcacctatat ttcgacggcg
     2221 gggggtgcct tcctcgaatg gttggagggc aaggagcttc cgggcgtcga ggcattgcgg
     2281 gttaattcgc ggcggcgtta aatcggccgc cgaatttttg tcacgaaccg atagggaaga
     2341 acggtgaacg agcagcaact cgcgcagacg gcgcaggctt tggttgcccc ggggcggggt
     2401 attctggcgg cggatgagag ctcttcgacg attgccaaaa ggttttccgc tatcggcgtc
     2461 gaatcgactg ccgactcgcg acgcgattat cgcgaattgc tgttccgttc gacccaggcg
     2521 atgcagaaat atatttccgg tgtcattctt tatgacgaga cgatccggca aaaagcgttg
     2581 gatggcacgc cgcttgtcac tttgatcgaa agggtcggct cgctcccagg catcaaggtc
     2641 gatgccggtg cgaaaccgct gccgggcttc cccagcgaaa cgatcacgga ggggctcgat
     2701 gggctcgctg gccggttgaa ggactattac gatctgggcg cgcgcttcgc gaaatggcgc
     2761 gcggtgatcg acatcgccga cggcattccg actcgcggcg cgatcctcgc caatgccgag
     2821 gcgctggcgc gctatgcagc gctctgccag gaggccaata tcgtgccgat cgtcgagccg
     2881 gaagtactga tggacggcaa tcattcgttg cagcgctgct atgaggtcac agagaccgtt
     2941 ctgcgtgcgg tcttcgccgc gctttatgag caacgcatct tcctcgaagg catgatcctc
     3001 aagccgaaca tggtcgtgcc ggggatgaaa tccggtcatc gcgcaagccc ggacgaggtt
     3061 gccgcggcga cgatccggct gttcaagaac agcgtcccgg ttgccgtgcc gggcattgcg
     3121 ttcctttcgg gcggccagtc ggattttgac gccaccgcga atctcgatgc gctcgtgcgc
     3181 gccgcgccgc cgtggccgat gaccttttcc tatggccgcg ccttacaggc tgcgccgcag
     3241 aaggcctggt cgggcaagcc ggccaatgtc ggggcggcgc agacggcttt cacccaccgg
     3301 gcctatatga acagcctcgc cgcgcttggc caatggtcgg aatcagccga gaaagcagcg
     3361 gcctgagcat cggaccgaaa agtgcgaagc ggttttcgga taaatccgat gcgacctgag
     3421 gctaccgcgc tttcgcccta tttcttggcg aggagttttg ccgcgcctcc attcggtgac
     3481 gcattgaagc aagattcctg atgtccgagg acgcaccgcg cctttacctg attacgccgc
     3541 cgctcgttga tgcggtggct ttttccgtga cctttgaggc ggcgctcgca gcggctgacg
     3601 tcgcctgcgt tttgctgcgt ttcgacccgc gggcgcatgc cggcgccgtc gcaaaaaaac
     3661 ttttggcgct ggcgcagccg cgcggggtcg cctgccttgt cggggatccg caattggcag
     3721 gggcgatcga agctgacggc gtgcatgttt cggcgcccgg cgacgcactc gatgccgcgc
     3781 tcaaggcgat gaagcccaat catatcgtcg gcgtcggcgg ccttgtcagc cgcgacgacg
     3841 caatggcggc gggcgaatcg ggtgccgatt atctgatgtt cggcggcccc gatacgccgc
     3901 aagcctttgg cgaaatccgt gagcgcatcg cctggtgggc ggagatattc aatctgcctt
     3961 gcgtcgccta tgcccaccag atcgccgagg tcgcgggctt cgtcgagatg gcggccgact
     4021 tcatcgcgct tgaaacagcg gtgtgggacg atccgcgtgg tccgggcgcg gcggtcgcgg
     4081 acgtaaatcg tctgctggcg cgcggacgca ctggcgcatg aacaggctga cgctttcggt
     4141 ctgctgcatg ttgctcgcct tttcggcgga gccagtttac gcggcgcaac ccgccgcagc
     4201 gcctgccgcg ctgcccgcaa tcgcgaaacc ggcaccggcc ttgcctcaac cgaatcccaa
     4261 tgccaacctg aatctcgatc tcgcctacgg tgcctatcag cgtggctatt acattacggc
     4321 gttgggcgaa gcgcgcaaac ggctggccgc caatccaaac gacggccccg cgatgacctt
     4381 gatcggtcag ctttttgacc aaggtctcgg cgtggtgacg aatgcgcgtg aggcggccgc
     4441 ctggtacaag gcggctgcgg aacacggcga ccgggaagga atctttcttt acgggctcgc
     4501 caagctgacc ggcaacggcg tcgcacagga ccgcgacggc gcggcagctc tcttcaccaa
     4561 agctgcggca atggacgatc cgggcgcgct gtataatctc ggcgtgctcg cgctcgaaac
     4621 gccgaaaaga gccccagatt ttcccaaggc ggcggattat ttccgccgcg ctgcggtgct
     4681 tggctcgcct gacgccgctt attcgctggc cctcttctat cgcgagggta agggcgcgcc
     4741 gcaagacgat gtcatggcgg cagaatggat gaagcaggct gccgatgaag ggcaagtttc
     4801 agccgaagtc gaatatgcga tcatgttgtt taatggagtt ggcgtcgcca aagacgaggc
     4861 ggccgcggcg aagcttttcc tcaaagcggc ggcgcggaat aatgtcgtcg cgcaggatcg
     4921 agcggcgcgg ctgcttgcgc ttggccgcgg catccaaaaa gatgtggtcg aggcaatgaa
     4981 atggcatctc ttagcgagcg ccgcgggcga aaaggatgcg tggctcgacg gcatcctcaa
     5041 ttcgctgacc ccggaacaga agatcgaggt tgaagtcgcc gtcaagcgcg ctctggcgcg
     5101 ttagcaccta tatggtgtca agtttcggca cgtcctcgcc caacgcggtt gcaagatcga
     5161 tctggt
//
LOCUS       DASWOG010000047         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_56039,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000047 DASWOG010000000
VERSION     DASWOG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56039"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="modB"
                     /locus_tag="VGB93_00875"
     CDS             <1..626
                     /gene="modB"
                     /locus_tag="VGB93_00875"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY0144713.1"
                     /translation="LGSLPFGILVAYGLARWHFPGKTLVTALVYLPLVMPPVVTGYLL
                     LIGFGRRGIFGQALADLGIVLSFRWTGAALACAIMGFPLMVRAIRLSFESIDPNLEKA
                     ASTLGANPVFRFLLVSLPLAVPGIVAGATLSFARALGEFGATITFVSNIPGETQTIPT
                     LIYTYTQEPGADEKALHLTLVSLLIAIVAIIASEIIQARGEKRLAGR"
     gene            623..1705
                     /gene="modC"
                     /locus_tag="VGB93_00880"
     CDS             623..1705
                     /gene="modC"
                     /locus_tag="VGB93_00880"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY0144714.1"
                     /translation="MIDVDVARRVGSFNLKVSFRNDNGITALFGRSGAGKSLTIGLIA
                     GLDRPDRGHVVLDGRALVDTDKGVFLPAYRRHIGMVFQDSHLFPHLGVRQNLLFGRWF
                     APRAARAISFDAVVEALGIGQLLGRTPAKLSGGERQRVAIGRALLSGPQLLLMDEPLA
                     ALDRARRLEILPLIERLRDDFKIPIVYVSHDVEEVARLASRVVVLDQGHVVAIGDPAE
                     VLPTALGDDARADRVSILAMHVGARDEAYGLTELIHPAGTVWLAGPHGTEGTRLRVVI
                     KATDVTLSTAAPQGLSVQGALKGRLGEIEALGPLAVAHIALDGDGTLLGMTTRRALDK
                     LGLGAGEAVFALVKTAALDERLIGGA"
     gene            1761..2519
                     /locus_tag="VGB93_00885"
     CDS             1761..2519
                     /locus_tag="VGB93_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY0144715.1"
                     /translation="MTEPVTAALLVIGDEILSGRTKDKNIGFTADFLAERGIDLREVR
                     VVPDVMDEIVAAVNALRHRYTYVFTTGGIGPTHDDITADAVGAAFGVPVVEDPRAIEL
                     LLQRIKAVDLNEARRRMARIPHGADLVENSISKAPGFKIGNVIVMAGVPAIMQAMLAA
                     VAPSLEAGPRTHAKSIAIGDIPEGAYAADLGHVAAQHPDLSIGSYPSIVDGRFHNEVV
                     VRGKDPAQIAAAVTAIEQIIADLRATGRDKYTIT"
     gene            2516..3010
                     /gene="gpt"
                     /locus_tag="VGB93_00890"
     CDS             2516..3010
                     /gene="gpt"
                     /locus_tag="VGB93_00890"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEY0144716.1"
                     /translation="MSDAPQRSFQVSWDQFHRDARALAWRLAATGPYTAVVGIARGGL
                     VPAAIVARELDLRVVESISVVSYDHTIQQGPPKIIKAIAPEIAAGQGAGVLVVDDLVD
                     TGATARVVRELLPKAHIATIYAKPLGRPYVDSFMTEVSQDTWIYFPWDLGLAFQAPIA
                     GGKG"
BASE COUNT          570 a         1063 c         1084 g          706 t
ORIGIN      
        1 ttctcggtag cctgccgttc ggcattctcg tcgcctatgg gctggcgcgc tggcattttc
       61 ccggcaagac gctcgtcacc gcgcttgtct acctcccgct cgtcatgccg cctgtcgtca
      121 cgggttatct gctgctgatc ggtttcggtc gccgcggcat tttcggccag gcgctggccg
      181 atctcggcat cgtgctgtcg ttccgctgga cgggtgcggc cctggcatgt gcgatcatgg
      241 gctttccgct catggtgcgg gcgatccggc tttcattcga gtcgatcgat ccgaatctgg
      301 agaaggcagc gagcacgctg ggtgccaatc cggtctttcg ctttttgctc gtcagcctgc
      361 ctttagctgt tccgggaatt gtcgcgggcg cgactttgtc gttcgcccgc gcgcttggcg
      421 aattcggcgc gacgatcacc ttcgtctcga acatacccgg cgagacgcag acgattccca
      481 cactgatcta cacgtatacg caagagccgg gcgctgacga gaaggcgctg catctgacgc
      541 ttgtctcact gctgatcgca attgtcgcga taattgcctc ggagatcatt caggcgcggg
      601 gcgagaaaag gcttgccggc cgatgatcga tgtcgatgtc gcgcgccgtg tcggcagttt
      661 caatctgaag gtctcgttcc gtaacgataa tggaatcacg gcgctgttcg gccgctccgg
      721 cgctggcaag tcgctgacga tcgggctcat tgcaggcctt gatcgaccag atcgcgggca
      781 tgtggtcctc gacggccgcg cgctggtcga tacggacaag ggagtttttc tgcccgccta
      841 tcgccgccat atcggcatgg tgtttcagga ttcgcatctt ttcccccatt tgggcgtccg
      901 ccaaaatttg ctctttgggc gctggtttgc gccgcgtgcg gcccgggcca tcagcttcga
      961 tgcggtcgtc gaggctttgg gaatcggtca gcttctcggc cgaacgccgg ccaaactttc
     1021 cggcggcgag cgccagcgcg tcgccattgg ccgtgcgctt ctgtcgggtc cacagcttct
     1081 gttgatggat gagccgcttg ccgcactcga cagagcccgg cggctcgaaa tcctgccgtt
     1141 gatcgaacgg ttgcgcgacg actttaaaat ccccatcgtc tatgtttcgc acgacgtgga
     1201 agaagtcgca cggctggcga gccgggtggt cgttctggat caggggcatg tcgtcgcgat
     1261 cggcgaccca gccgaggtct tgccgacagc gctcggcgat gatgcgcgtg ccgaccgcgt
     1321 cagcattctc gccatgcatg tcggcgcgcg cgacgaggcc tatgggttga cggagctcat
     1381 ccatcctgct ggcaccgttt ggctcgccgg tccgcatggc actgagggca ccaggctccg
     1441 cgtcgtgatc aaggcaacgg atgtcacact ctcgacggcc gcgcctcagg gcctcagtgt
     1501 ccagggcgcg ctcaaaggcc ggctcgggga gatcgaggcg ctgggtccgc ttgccgttgc
     1561 ccatatcgcg ctcgatggtg acggcacact gctcgggatg acgacccgac gcgcgctcga
     1621 taagctcggc ctcggtgccg gggaggcggt tttcgcattg gtgaagaccg ccgctctcga
     1681 cgagaggctc atcggcgggg catgatgcgg ccaaggccaa ccttggcctg agccatacgc
     1741 taagatgcgg agttttatat atgacggagc cggttaccgc ggcgctcctc gtcatcggcg
     1801 acgaaatcct gagcggccgc accaaggaca agaacatcgg cttcaccgcc gattttctgg
     1861 ccgagcgcgg catcgatcta cgcgaagtgc gggtcgtccc cgacgtgatg gacgagatcg
     1921 tcgccgcggt caacgcgctg cggcatcgct atacctatgt cttcaccacg ggcggcatcg
     1981 ggccgacgca tgatgatatc accgccgatg cggttggtgc cgccttcggc gtgccggtgg
     2041 tcgaagaccc acgcgcgata gaattacttt tgcagcgaat caaggccgtc gatctcaacg
     2101 aggcgcggcg gcgcatggcg cgcattccgc atggcgccga tctggtcgag aattccatct
     2161 ccaaggcgcc gggcttcaag atcggcaacg tgatcgtcat ggccggcgtg ccggcgatca
     2221 tgcaggcgat gctcgccgcc gtggcgccga gccttgaagc cgggccgcgc acgcacgcga
     2281 aaagcattgc cattggtgat attccggaag gcgcgtatgc cgctgacctg ggccacgtgg
     2341 ccgcgcaaca tccggacctc tcgatcggct cctatccgtc gatcgtcgat ggccgctttc
     2401 acaacgaggt cgtcgtgcgc ggcaaggacc cggcacagat cgctgccgcc gtcacggcga
     2461 tcgagcagat catcgccgat ctgcgcgcca ccggcaggga taaatatacg atcacatgag
     2521 cgacgcgccg caacgatcct tccaggtctc ctgggaccaa ttccaccgcg acgcccgtgc
     2581 gctggcctgg cgccttgcgg cgacggggcc ctataccgcc gtggtcggca ttgcccgcgg
     2641 cggtctggtg cccgccgcca tcgtcgcgcg cgaactcgat ctgcgggtgg tcgagtcgat
     2701 ttcggtcgtc agctacgatc acaccatcca gcaggggccg ccgaagatca tcaaggcgat
     2761 tgcgcccgag atcgcggccg ggcagggcgc gggcgtcctc gtggttgacg atctggtcga
     2821 tactggcgcg acggcgcggg tggtgcgtga acttctgcct aaggcgcata tcgcgacgat
     2881 ctacgccaag ccgctcggcc ggccttacgt cgatagtttc atgaccgagg tttcgcagga
     2941 tacgtggatc tattttcctt gggacctcgg cctcgccttc caggcgccga tcgccggcgg
     3001 caagggatag ttcaagcgat agatttgcgt cgagcgccgc ggcgctttat ggcgaggtta
     3061 cgcgggcgtg gcgaaattgg tagacgcaaa ggacttaaaa atctttttgt cccctcggac
     3121 gcttctttta aaccccctgg catcctcttg aaagtagggt gaaaagaggt ccaaggacct
     3181 tgcgaacccc tttcacatcc ccatttaaaa tctatactgc atctatacgg atgcgggggt
     3241 gggagcggag gactttcagg aacatgcgtg ttcgattgac cgaagcgctg gtcgctaaaa
     3301 ccaccggaaa tggcaagctc accttcatcc gcgacgaccg cttgatcggc ttcgcgttac
     3361 aggtcactcc cgccgggacc aagagctttg ttgccgaggt cttcacccag ggtcggtccc
     3421 ggc
//
LOCUS       DASWOG010000048         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_202636,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000048 DASWOG010000000
VERSION     DASWOG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202636"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGB93_00895"
     CDS             <1..703
                     /locus_tag="VGB93_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEY0144717.1"
                     /translation="DLRAEVAALTGAVSGVLRIACLPTFGRRYVMPALEALMANHPEL
                     RVDLDLDERIADAVAGRVDVVLRVGHLEDSSLIATKLASHERRLVASPHYLEQFGHPA
                     SLSALGNHRLLDKIHGADLLGWSNLFECKAGHLAGGQDVFRSDDFETLRNAALYGFGI
                     ALLPSWVVGQDIASGTLILLLPDLVDARGDAGGIYLLRALADAPAKVTAFTTSLRTVI
                     GSPPIWDRGCDMRVA"
     gene            complement(700..1950)
                     /locus_tag="VGB93_00900"
     CDS             complement(700..1950)
                     /locus_tag="VGB93_00900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY0144718.1"
                     /translation="MEKMFRRFIAALIKTGSLEVETASGARYQFGDGSGKNVRLRFAD
                     KAAQTAVLLNPTLRFGELYMDGRVTVDEGTIYDALAAICQNIDHLERIWWLQALTTAR
                     GRLQHIFKANSSVRARRNVAHHYDLDRRLYKLFLDDDMQYSCAYFENGAATLDDAQLA
                     KKRHIAAKLMIEKGDRVLDIGCGWGGLALYLARICGAKVTGITLSREQLAFARERASE
                     ADQSNAIEFRLQDYREIDERFDKIVSVGMFEHVGPEFYATYFQQVAKLLQPGGMALIH
                     TIGSSRVQYEPDPWINKYIFPGGYIPSLATIMPAIERAGLYVTDIEILRLHYAETLRA
                     WRARFLAHRADAAALYDERFCRMWEYYLAASEAAFRYTGEVVFQIQLAKKVDAVPLTR
                     DYMLRAEAELRRRDSAGSGLRIAG"
     gene            2167..2739
                     /locus_tag="VGB93_00905"
     CDS             2167..2739
                     /locus_tag="VGB93_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144719.1"
                     /translation="MQVPHVLRASAFIAAVTFAVPALAFGGGHGGGGGHGGGFGGGGF
                     HGGGGGFHGGGFGGGGFHGGGWHGGGGGWHGGGWRGGYGGWGYPGYGYGYGYPYGYDG
                     GYSDWGYGYGDPGYDYGYDNGYGYGPGVYGQGDGQNPGAYTAPPQANGQLGQYCRTQV
                     KTCQLYHASNVGAGCSCRTGSSHAYGQVTP"
     gene            complement(2779..>3078)
                     /locus_tag="VGB93_00910"
     CDS             complement(2779..>3078)
                     /locus_tag="VGB93_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 5-epimerase"
                     /protein_id="HEY0144720.1"
                     /translation="APGSTHGAIAGDSLSPVAPWRVVNIGAGQPVELEDFVASVEHAL
                     GQTAKCKLLPMQIGDVPATFANTDLLKALTGYRPSTPIDVGVPRFVDWYRTHYRA"
BASE COUNT          560 a          880 c          970 g          668 t
ORIGIN      
        1 agatctccgc gccgaggttg cggccctcac cggcgcggtc agcggcgtgc tgcggatcgc
       61 ttgcctcccg acgttcggtc ggcgctatgt gatgccagcc cttgaagcgc tgatggctaa
      121 ccatccggaa ttgcgtgtcg acctcgacct cgatgaacgt atcgccgatg ctgttgccgg
      181 acgggtggat gtggtgcttc gcgtcggcca tctagaggac agttctctga ttgcgaccaa
      241 gctcgcctcg catgaacggc ggctcgttgc gagcccgcac tatctagagc agtttggtca
      301 tccggcttcg ctttccgcgc tggggaatca ccgcttattg gacaagatac acggcgctga
      361 tctgcttggc tggtcgaacc tatttgaatg caaagccgga catctcgccg gaggccaaga
      421 tgttttccgc agcgacgact ttgagacgct acgaaatgca gcgctctacg gctttggcat
      481 cgcgctgctg ccgagctggg ttgtcggcca ggatatagcg tctggcacgc taatattgct
      541 tttgccggac ctcgtcgatg caaggggcga cgctggcgga atctatctcc tcagagcact
      601 tgcggatgcg cccgcaaagg tgaccgcgtt tacaacgtcg ttgcggaccg tgatcggatc
      661 gcctccaatt tgggatcgtg gctgcgacat gcgcgtcgcc tagccagcga tccgaaggcc
      721 agagccggcg ctatcgcgac gccgcaattc cgcttccgca cgcaacatat aatctcgcgt
      781 cagcggaacg gcgtcgacct ttttggcgag ctggatttga aacacgactt cgccggtgta
      841 gcggaaggca gcttccgatg cggcgagata atattcccac atccgacaga aacgctcgtc
      901 gtagagcgct gcggcgtcgg cgcgatgcgc aaggaagcgt gcgcgccacg ccctgagcgt
      961 ctcggcatag tgcagccgca gaatttcgat atcggtcacg tagaggccgg cgcgttcgat
     1021 cgccggcatg atcgtcgcga gcgaggggat atagccgccg gggaagatgt atttgttgat
     1081 ccacggatcc ggctcgtact ggacccgcga cgaaccgatt gtgtggatca gcgccatgcc
     1141 accgggctga agcagcttgg cgacttgttg gaaataggtg gcataaaatt ccgggccgac
     1201 atgctcgaac atgccaaccg aaacgatctt gtcgaaacgc tcgtcgatct cgcgatagtc
     1261 ttgcaggcgg aattcgatgg cattcgattg atccgcttcg ctggcgcgtt cgcgtgcgaa
     1321 ggcgagttgc tcgcgcgata aggtgatgcc cgtgactttg gcgccgcaga tacgggcgag
     1381 ataaagcgcg aggccgcccc acccgcagcc gatatcaagc acgcggtcgc ctttttcgat
     1441 catcagtttc gcagcgatat gacgcttctt cgccagctgc gcgtcgtcga gagttgcggc
     1501 gccgttctcg aaataggcgc aggaatattg catgtcatcg tcgagaaaga gtttgtagag
     1561 ccgccgatct aggtcgtagt gatgcgcgac gttgcggcgc gcgcgcactg aactgttggc
     1621 tttgaaaata tgctgcaaac ggcctcgtgc ggtcgtcagc gcttgcagcc accagatccg
     1681 ttcgagatgg tcgatgttct ggcaaatggc ggcgagcgcg tcgtaaatcg tgccctcgtc
     1741 gaccgtgacc cggccatcca tatagagttc gccgaaccgg agcgtgggat tgagcaagac
     1801 tgccgtttgc gcggctttat cggcgaagcg cagacgtaca tttttgccgc tgccgtcgcc
     1861 gaactggtat ctcgctcctg acgcagtttc gacttcgagc gaccccgtct tgataagggc
     1921 ggcgataaac cgccggaaca tcttctccat ctcacacctc gtcgccgagg ctcattgcgc
     1981 agcaccgacc cgttcgacgg ctccaccgtg tgcaaacgca cagcccggat atctgaccct
     2041 gtgttcgcct cgcgacggcg cctaacgcct cttttaactc ccgacttcac gcctatgttt
     2101 gacgttcatc aatgtatgga tgcaaccgaa gccgcatctg aacatttaag aactggagtt
     2161 tcaaagatgc aagtgcctca tgttttgcgt gcttcggctt tcatcgcagc ggtgacattc
     2221 gccgttcctg cgcttgcatt tggcggcggc catggcggtg gtggtggtca tggtggtggc
     2281 ttcggcggtg gcggattcca cggtggtggc ggcggctttc atggcggtgg ctttggcggc
     2341 ggtggttttc acggtggcgg ctggcacggc ggcggtggtg gctggcacgg tggtggttgg
     2401 cgcggcggct acggtggatg gggttatcct ggctatggtt acggctacgg atatccctac
     2461 ggttatgacg gcggttattc agattggggt tacggttatg gcgatcctgg ttacgattac
     2521 ggttatgata atgggtatgg ctatggtccg ggcgtctatg gacaaggcga tggtcaaaat
     2581 cccggtgctt acaccgcgcc gccgcaggca aacggacaac tcggccaata ttgccgaacc
     2641 caggtgaaaa cctgccagct ctatcacgct tcgaatgtcg gcgcgggttg ctcgtgccgg
     2701 accggttcgt cgcacgccta cgggcaggtt acgccttaga ggtgctcctg catcgaccga
     2761 agggcgtctg gccttctgct aggcgcgata atgcgtgcga taccaatcga cgaagcgcgg
     2821 cacgccgaca tcgatcggcg ttgaggggcg atagcccgtc aacgccttga ggagatcggt
     2881 gttggcgaag gtcgcgggga catctccgat ctgcatcggc agcagtttgc acttggccgt
     2941 ttgcccgagc gcatgttcga cggacgcgac gaaatcctcg agttctaccg gctggccagc
     3001 accgatattg acgacgcgcc acggcgcaac cggcgacagg ctatcgccgg caattgcccc
     3061 gtgcgtgctt cccggcgc
//
LOCUS       DASWOG010000049         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_166031,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000049 DASWOG010000000
VERSION     DASWOG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166031"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGB93_00915"
     CDS             <1..487
                     /locus_tag="VGB93_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetaldoxime dehydratase family protein"
                     /protein_id="HEY0144721.1"
                     /translation="MDPVGELTLKQGTPAKGGRVIVTGHQNLTLIRSGEDWSRTEDEE
                     RRTWFDDIQPVLLEGMNYLRDDGLEVGCYCNRYCYHMEEDGKLQEKGFGVSLWRSLSN
                     LESWAESHPTHLQIFVTFNRLAKKFKELVLYHEVSVFDAGAQYFEYINCHPKTGVMRA
                     L"
     gene            complement(566..1477)
                     /locus_tag="VGB93_00920"
     CDS             complement(566..1477)
                     /locus_tag="VGB93_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0144722.1"
                     /translation="MNEHVRETRAQPLRDPTNKYPKPPYKKQTQPWPGLACKMDPPPD
                     HGETSYRGTGRLLGRKALITGGDSGMGRAAAIAYAREGADVAINYFPTEEEDAQEVMR
                     LIKEAGRTGVALPGDLRDEAFCGKLVEDAIGVLGGLDIIVSNAGRQQTHDSILDISSE
                     DFDATMKTNIYAPFWIIKAALALLRPGAVIIATTSEQAYDPSPDLYDYAQTKAATMNF
                     VKSLAKQLGPKGIRVNGVAPGPIWTPLQVSGGATMEKLEQFGGHAPLGRAGQPAELAS
                     IYVQLAAEDASYATGQVYGAAGGGGQP"
     gene            complement(1584..1763)
                     /locus_tag="VGB93_00925"
     CDS             complement(1584..1763)
                     /locus_tag="VGB93_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144723.1"
                     /translation="MNVARLLRRTARRLASWKKADRASTRADLGDLEEQIRIMPDAEF
                     AEYLRLHTKSHRWKV"
     gene            complement(2044..2538)
                     /gene="fae"
                     /locus_tag="VGB93_00930"
     CDS             complement(2044..2538)
                     /gene="fae"
                     /locus_tag="VGB93_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEY0144724.1"
                     /translation="MGDVKIDRVLVGEALVGDGNEVAHIDLIMGPRGSAAETAFCTTL
                     TNQKVGDNALLALVRPNLMTKPATVMFNKVDIKNGDQAGKMFGPAQTGVGKAVIDCVK
                     SGVIPKDEAANIFICVGVFIHWEAKDDAKIQSWNYAATKLAIERAVKGEPSIDDVISQ
                     ENVT"
     regulatory      2657..2885
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3064..4140
                     /locus_tag="VGB93_00935"
     CDS             3064..4140
                     /locus_tag="VGB93_00935"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0144725.1"
                     /translation="MLEQTSAAKLTPPLDLRKIGAQVDHWYPLAWSREVKTGRPFAGS
                     FAGEPIVLIRPKEGALFALEDRCAHRQVPLSRGVVNGNVVRCGYHGWSYNSEGHCVDV
                     PYLGKGKLPNGVRAYPCHEVDGVIFIWPGSATPDAPPTKIGAGGDKSYKTRLFGEVVA
                     CHFTFMHENLMDMNHQFLHRSTTGKVYPRYLDRRIGDDWMEVDYSFMRPDEKPPLGEA
                     VIVGAMRGQSDKAQDLMTIRTEYPHQTLKFWTSEGDPVLDVWLGYTPVDKEQRSNRTF
                     ISLSVRRPKIPFLLDAVWPIMAMFTNRVFREDREVVEMEQAAHDSQGSDWNQEVFPPI
                     QDLRALLAANGQPIAPPATPAETN"
     gene            4360..5214
                     /locus_tag="VGB93_00940"
     CDS             4360..5214
                     /locus_tag="VGB93_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144726.1"
                     /translation="MGVVTGASGGIGRWIALGLARAGCHVVLICRDSERGNAVAAWIA
                     SQVSGSSTEVLLADLSSLREAHRLGLEITAVHPRVNVLVNNAGMFAARRQVTAEGHDA
                     VLAVNHLAPFVLTDALEGSLRAGAPSRIVNVGSSTSDRAKIDPEDLELTRHWGMVRAY
                     SQSKLAMMMATFARAEHLRGSGVVANVVHPGAVASGLIKERGPIGAAWRRMAPFLRTE
                     EQGADTPLHVALAPDWAGITGAYAKDRAAVRPNLRALDAGLVKKVDSVTRVLVEGTIG
                     ESVDLNRP"
     gene            complement(5211..>5941)
                     /locus_tag="VGB93_00945"
     CDS             complement(5211..>5941)
                     /locus_tag="VGB93_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144727.1"
                     /translation="LDTPADQFQRVTDVTYLGTVNGTRVALRRMLPRDRGSIINVCSA
                     VAYHGMPLLSSYSGAKHAVRGFGQSICAELSEEGSRVRLTTIFPPAVNTPFFEHAISH
                     MGRPGRPIAPVYQPETVAEAIYLAAMTKRREMPVSFTTLAFSIGVRLVPGLVHRAIRK
                     LGYSGQLADRTMSPASQDTTLFAASDRASPARGAFSAGARSGSVHIRLLYALAALARR
                     SRVATHATPSPVEDPAPAIVEEPV"
BASE COUNT         1183 a         1719 c         1789 g         1250 t
ORIGIN      
        1 gatggatccg gtgggcgaac ttactctcaa acaaggcacg ccggccaaag gcggccgtgt
       61 gattgtgacc ggtcaccaga atttgaccct catccggtca ggcgaagact ggagccgaac
      121 cgaagatgag gaacgccgga cctggtttga cgatattcaa ccggtgctcc tcgagggaat
      181 gaactatttg cgcgacgacg gtctcgaggt cggctgctat tgcaaccgct attgctatca
      241 catggaagaa gacggcaaac ttcaggaaaa aggtttcggt gtgagcctct ggcgctcatt
      301 gtcgaatctt gaatcctggg cagaatcgca ccccacgcat ctgcagattt ttgtcacgtt
      361 caaccggctc gccaagaagt tcaaagagtt ggtgctttat cacgaagtgt ccgtcttcga
      421 tgcgggcgct cagtatttcg aatacatcaa ctgtcatcca aaaacgggcg tgatgcgcgc
      481 gctctgaatt tagagcactt ctatgatcga tgggtgtctt cccgaaacac ccatcgatct
      541 aaactagcaa tatctcaatt gtcgcctacg gctggccgcc gccgcccgcc gctccataca
      601 cttggccggt ggcatagctc gcatcttccg ctgcaagctg cacatagatc gacgccaatt
      661 cggctggctg gccggcgcgg ccgagcggcg cgtgaccgcc gaattgttcg agcttctcca
      721 tcgtcgcgcc acctgaaacc tgcaacgggg tccatatggg accgggagca acgccattga
      781 cgcggatgcc tttcggccca agctgctttg ccagcgattt cacgaagttc atcgtcgctg
      841 ctttggtctg cgcataatca taaagatcag gcgaagggtc gtaagcctgc tcagacgtgg
      901 tggcgatgat cacagcgccg ggacgcaaaa gggccaaagc cgccttgatg atccagaacg
      961 gcgcgtaaat gttggtcttc atcgtcgcat cgaaatcctc cgatgaaata tcgaggatcg
     1021 agtcatgcgt ctgctgacgt ccggcattgc tgacgataat gtccaatccg ccgagtacgc
     1081 cgatcgcatc ttcgacaagt ttgccgcaga aggcttcgtc gcgcagatcg ccaggcagcg
     1141 cgacgcccgt gcgtcccgct tccttgatga ggcgcataac ctcctgtgcg tcctcttctt
     1201 cggtcggaaa ataattgatc gccacatctg cgccttcgcg cgcataggcg atcgcggcgg
     1261 cacggcccat gccggaatcg ccgccggtga taagcgcctt gcggccgagc aggcggccgg
     1321 tgccgcgata gcttgtctca ccgtggtctg ggggcggatc catcttgcag gctaagcccg
     1381 gccaaggctg cgtctgcttc ttatagggcg gcttcggata tttgttcgtc gggtcgcgca
     1441 gaggctgcgc gcgggtttct ctcacatgtt cattcatggt tgctctccgg gttcggccgg
     1501 agaacgcgga ggcgcgtcgc aggttgccaa ccgcgcgagg cttacgccat cacacagatt
     1561 cgatcggtca aggccgaggc tgcctagacc ttccatctgt gcgacttcgt atgcaggcgc
     1621 agatattcag caaattctgc gtccggcatg atccggattt gctcttcgag atcacccaaa
     1681 tcggcgcggg tgctcgcgcg atcggccttt ttccaagaag caagccgccg cgcagtgcgg
     1741 cgcaacaaac gggcgacgtt catgtccaat cctccgcctt ctcgaagaat agggcagtgg
     1801 gtcatttcgc caattgtcca aaaggctaaa gaccgcgccg actgcccgag gttctcccgc
     1861 gtatacggct ccccactttg ggattgccgc atgcggatgg aaaacggtca aggacgaaaa
     1921 aggctgcgcc agcgcattat tttaaagact ggtcgcagca attggcggcc cggaagcttc
     1981 cgatccgaga atcgcagcat gccgaccgct ttatggccct caattatggg aggccgatgg
     2041 gtgctaggtc acattttctt gggaaatgac gtcatctatt gacggctcgc ctttgacagc
     2101 gcgttcgatc gcgagcttgg tcgccgcgta attccagctc tgaattttgg catcgtcctt
     2161 tgcctcccag tgaatgaaga cgccaacgca aataaaaata ttggcggctt catctttggg
     2221 aatgacgccc gatttcacgc aatcgatgac ggcttttccg acgccggttt gcgcgggacc
     2281 aaacattttt ccggcttgat ctccattctt gatatcgacc ttgttgaaca tcacggtcgc
     2341 cggcttcgtc atgaggtttg ggcggacaag tgccagaaga gcattgtcgc cgactttctg
     2401 gttggtgagg gtcgtgcaga aagccgtttc ggcggcactt cctctcggtc ccatgatcag
     2461 atcaatatgt gcaacttcgt tgccgtcacc cacgagcgct tcgccaacca gcacgcgatc
     2521 aattttcacg tcgcccattt aatcctcctt tggataaatg tttctttgct tgcgcgaaca
     2581 gctatatcac gctccgcgct atttcgccgc gcgaacatcg gatccagagt gaccttgact
     2641 cacccatcca ttcgcccata ttcgaaattg tcgtggtctt cggcgaactc aattcgcgcg
     2701 aagctaagag ggaagctggg accgatcgga cgctgttcgt tcgagattaa agccggcgct
     2761 gcccccgcaa ctgtaagcgg cgagttcgag tccatgcatg tcactgggaa gatatgatcc
     2821 tgggaagacg gactcaaaac aagaacccgc aagccaggag acctgcctcg gcaatatatc
     2881 gtcctcgggc ggggtgtctc ggtggtgcgc ttcgacctcc acggcgcgca gcgaagttgc
     2941 agacgagtca catgtgcatc gccgctcgcg cttgaggggt gatgcatgtt tttgactctt
     3001 ccacatggcg gccacggcat tccgcagggc aacctatctc atttctcgcc gcggagcctt
     3061 tgaatgcttg aacaaacgtc tgcggctaag ctgacacccc cgcttgatct ccgcaagatc
     3121 ggcgcgcagg tcgaccattg gtatccgctt gcctggtccc gcgaggtgaa aactgggcgc
     3181 ccgtttgccg ggagtttcgc tggcgagccg attgtgctca tccgccccaa ggagggcgct
     3241 cttttcgcgc tggaagaccg ctgcgcgcac cggcaagtgc cgttgtcgag gggcgtcgta
     3301 aacggcaacg tcgtgcgatg cggctatcac ggctggagct acaattcgga aggccactgc
     3361 gtcgacgttc cctatctggg aaaaggcaaa ttgccgaatg gcgtgcgagc ctatccctgc
     3421 cacgaggtcg atggggtcat cttcatctgg ccgggctcag caacgccgga tgcaccgccc
     3481 acgaagattg gcgcgggcgg cgataaatct tacaaaaccc ggctttttgg tgaagtcgtc
     3541 gcgtgccatt ttacgttcat gcatgagaac ctcatggaca tgaaccatca gttcctgcat
     3601 cggagcacga cgggcaaggt ttatccgcgt tatctcgatc gccgcattgg cgatgattgg
     3661 atggaagtcg attatagttt catgcgcccg gacgagaaac cgccgctcgg cgaggccgtc
     3721 atcgtcggcg cgatgcgcgg acagtccgac aaggcgcagg atcttatgac gattcggacc
     3781 gaatatccgc atcagacgct caagttctgg acgtctgagg gggacccggt tctcgatgtc
     3841 tggctcggtt acacgcctgt cgacaaagag caaaggtcca acaggacgtt catttcattg
     3901 tcggtgcgcc gtccgaaaat accgttcctt ctcgatgcgg tctggccgat tatggccatg
     3961 ttcaccaatc gcgtcttcag agaggatcgc gaggtcgtgg agatggaaca agcggcgcat
     4021 gacagccagg gctctgactg gaatcaggaa gttttcccgc cgatccagga tttgcgggct
     4081 ctgcttgccg ccaacggaca acccattgcg cctccggcaa caccggctga aacaaattga
     4141 gcagcttgcg gctatgcgta ctcgaagcca tcgccgcgcg tccgaagccg ctggcgaaaa
     4201 atgggcagcc gctacgtttc cgttccggca ccttccatgg acttcgtgaa ttttattgga
     4261 cgtgactccg cgatcgccag cgctaaaagc ccaaaggatc ctatggaagc ggtcgatatg
     4321 gcgcgaggag cgtaacgatt gagtgagaca ggtcggcccg tgggtgtcgt caccggtgct
     4381 tctggcggga tcgggcgatg gattgcattg gggctggcgc gagccggttg tcatgttgtc
     4441 ctgatttgcc gggactcgga acgcggcaac gctgtcgccg cctggatcgc ttcgcaggtg
     4501 tcgggttcca gcacggaagt gcttctggcc gatctgagtt cgctgcgtga agcgcatcgt
     4561 ctgggtttgg agatcactgc ggtacatccg cgggtcaatg tactcgtcaa taacgccggg
     4621 atgttcgccg cgcggcggca ggtgacggcg gaaggccacg atgccgtgct cgcggtgaac
     4681 catctcgcgc ccttcgtttt gactgatgcc ctggagggct cgctacgggc aggcgcgcct
     4741 tcacgcatcg tcaatgtcgg ctcgtccact tcggaccgcg cgaagatcga tcccgaggat
     4801 ctggaactca cgcggcactg gggaatggta cgggcctata gccagtcaaa gctcgcgatg
     4861 atgatggcga ccttcgcgcg tgcggagcat ttgcgcggca gcggggtggt cgccaacgtc
     4921 gtgcatccgg gcgcggtcgc ctccggactg atcaaagaac gggggccgat aggcgccgct
     4981 tggcggcgga tggcgccctt tttgcggacc gaagaacagg gtgcagacac gccgctgcat
     5041 gtggcgttgg caccggactg ggcgggaatc accggtgctt atgcgaaaga ccgcgccgct
     5101 gttcgtccca acctgcgcgc tctggatgca gggctggtga aaaaggtcga ttccgtcact
     5161 cgggttttgg tggagggaac gatcggtgag tccgtcgatt tgaaccggcc ctaaaccggc
     5221 tcctcaacaa tggcaggtgc cggatcttct accggagagg gcgtggcatg cgttgcgacg
     5281 cggcttctcc tggccaacgc ggcgagggca tacaagagac gaatgtgtac actccctgac
     5341 cgggctcctg cgctaaaggc accgcgcgcc ggagaagcgc gatccgaggc tgcgaacagc
     5401 gtggtgtcct gcgatgccgg cgacatcgtc cggtcggcga gctggccgct atagccaagc
     5461 ttccggattg cgcgatgcac gaggccggga acgagacgca cgccgatcga aaacgccagc
     5521 gtcgtgaaac tcaccggcat ctctcgccgc ttcgtcatcg ccgccagata gatcgcctcg
     5581 gcgacggtct cgggttgata gaccggcgcg attggacgtc cgggccgccc catatgagat
     5641 atcgcatgtt caaaaaaggg agtgttgacc gctggcggaa agatcgtcgt cagccgcacg
     5701 cggctgcctt cctcggacag ctcggcgcag atcgattggc caaaaccgcg cacggcatgc
     5761 tttgcccccg aataggacga tagcaacggc ataccgtggt aagcgacggc ggagcacaca
     5821 ttgatgatgc tgccgcgatc gcgcggcagc atccggcgca gtgctacgcg cgtcccgttg
     5881 accgtgccga gataagtgac atcggttaca cgctggaatt gatcggcggg cgtatcgagg
     5941 a
//
LOCUS       DASWOG010000050         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_129428,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000050 DASWOG010000000
VERSION     DASWOG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129428"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..732
                     /gene="aroQ"
                     /locus_tag="VGB93_00950"
     CDS             286..732
                     /gene="aroQ"
                     /locus_tag="VGB93_00950"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0144728.1"
                     /translation="MKAIHVLNGPNLNLLGSREPDVYGHETLADIEARLAAAAARENV
                     TLSFRQSNHEGDLVTWIQDAGRTGEPVILNAGAYSHTSIAIFDAIKGSNAHVIEVHLS
                     NIHAREHFRHHSRLSAAARGVIVGFGSRSYDLALQALLFAPTTGES"
     gene            736..1218
                     /gene="accB"
                     /locus_tag="VGB93_00955"
     CDS             736..1218
                     /gene="accB"
                     /locus_tag="VGB93_00955"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0144729.1"
                     /translation="MTEKKPGIPAPPTPIDPQLVETLGEIANRLDLSEIEVAQGELKI
                     KVKRQSANAAPVAVAVPALSTLQAHALAPAANEAALAIAANAVKSPMVGTAYLRPSPE
                     SAPFITIGTQVKAGEKLLLIEAMKTFNEIVAPQAGTVTSILVEDGQPVEFGQPLVVVE
                     "
     gene            1240..2592
                     /gene="accC"
                     /locus_tag="VGB93_00960"
     CDS             1240..2592
                     /gene="accC"
                     /locus_tag="VGB93_00960"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0144730.1"
                     /translation="MFDKILIANRGEIALRILRAAKELGIATVAVHSTADAEAMHVKL
                     ADESVCIGPPPARESYLNIPALLSACEITGAEAVHPGYGFLAENARFAEILEEHQIDF
                     IGPSAEHIRLMGDKIEAKRTAKKLGIPCVPGSDGAVEADEALRIAKEIGYPVIVKASA
                     GGGGRGMKVARSAKELSNALATARSEAKAAFGDDAVYLEKFLTNPRHIEVQILGDGQG
                     HAIHLGERDCSLQRRHQKVFEESPSPALNLAQRLEIGEICANAMREMNYKSAGTIEFL
                     YDDGKFYFIEMNTRIQVEHPVTEMVTGLDLVNEQIKIAAGSPLSISQSDVTMLGHAIE
                     CRINAEDPKTFRPSPGKIAYYHPPGGLGVRVDSAAYAGYTIPPYYDSLIGKLIVHGRT
                     RDEAMLRLRRALDEFIVDGIETTIPLFRALVRNADVQNGEYNIHWLEHFLEKGGMDKA
                     "
     gene            complement(2599..>5224)
                     /locus_tag="VGB93_00965"
     CDS             complement(2599..>5224)
                     /locus_tag="VGB93_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0144731.1"
                     /translation="GVAGAEVFGGEEKSTQILVHPDRLIRYGLGMEDVLAAARNATGV
                     GGAGFISTPNQRITLQTDTSGVDSEDIARTIVPLRNGGRVTLGDVADVIEAPEPSIGA
                     AAIMAQPGVVMNIDEQYGANTLEVTDAIEAALADLNPSLKAQGVTLHTGLFRPANFII
                     AATNNLRSSLLIGGVLVIAVLILFLFDLRAAAICCTAIPISLLAAVVILQRSGITLNT
                     MTLGGLAIALGEVVDDAVIGIENITRRLRENELLPQPRPAGRVVLEATFEVRSAVVYA
                     TFAVLLVFLPVVTLSGVAGRLFAPLGLTYIYAVVASLIVALTVTPALSLLLLPHHAKA
                     HVPPVVRWTRGGYERLLRVIVGAPRVAIFFAAVLTIGGLALLPLFGETFLPDLQEGHF
                     IVHMTSMPGTSTAEGLRMGARIADAMMKLPMVKTVAQRVGRVEQAAAGDTHGTHQSEF
                     EVELNPISGSAAMNAKQALLNHLLDFPGVNISANTFLTERVNETFSGYSTPVAVNVYG
                     SDLGAINDTAERVVTILRRVQGAASVELESPLGLPQVSIRLRSADLRRFDIAPANVLD
                     VVRTAYQGDVVGQTYEADRVFNVIVRLAERRGGDVTGIGDLTLRAPDGTYVRLAQVAD
                     IEPTSGLYLIEHQGGRRLQSITLDVQGRDPASFVKDARAALRKLKLPPDTYIVFTGAA
                     EGQAQAQQDLMLKSIFAGVGIVLLLSVVTRNWRNLLVTLANLPFALVGGVVAVYFSGA
                     VLSLGSLVGFVTLFGITLRNSMMMISHYEHLVSVDARTWDKDTAVHGAGDRLAAVLMT
                     SLVTGLGLLPLAIGMNTPGREIEGPMALVILGGLFTSTALNLFILPPLSLRFGRFVGT
                     PDDELADAPQAKDAVTAL"
BASE COUNT         1123 a         1600 c         1585 g          916 t
ORIGIN      
        1 ccggcggcca aggcgggcct ggaggaaggg ttcaaactcg gccatgtgct gcagattgag
       61 ggcacgccgt cctatatcgt cggcgatacc gtcgtcgatg gcgctgtcgg ttatgccgag
      121 ttgcaaacca aagtcgacaa catcaggaag tgcggcaagg ccatttgttc ctaacctgtt
      181 cgcggacagc ccctttgaag gctgcccgcg tgcatctcgg gctttctttg ctgcgggaag
      241 ccggttaagg aagcttcgcg ctggccccct caataaagcc caatgatcaa ggcgattcac
      301 gtcctcaacg gacccaacct caatttgctc ggaagccgtg agccggatgt ctacggccac
      361 gagacgctcg ccgatatcga ggcacgcctt gccgcagcgg cggcccgtga aaacgtgacg
      421 ctgagcttcc gtcaatccaa tcacgagggt gatctcgtca cctggattca agacgcgggg
      481 cgcaccggcg agccagtgat cctcaacgcg ggtgcctaca gccacacgtc gattgccatt
      541 ttcgatgcca ttaagggttc aaacgcccat gtcatcgagg tgcatctgtc gaatatccat
      601 gcgcgcgaac attttcgcca tcattcccgc ctatccgcgg ccgcccgtgg cgtgatcgtg
      661 ggatttgggt cgcggtctta tgaccttgcc ttgcaagccc ttttgttcgc cccaacgacc
      721 ggcgagagtt aaaagatgac agagaagaaa ccgggcatac cggccccgcc caccccaatc
      781 gatccgcaac tcgtcgagac tctcggcgaa atagccaatc ggctcgacct cagcgagatc
      841 gaggtcgccc aaggcgaact gaaaatcaag gtcaaacgcc aaagcgcaaa cgccgcgccc
      901 gtcgcggttg ctgtgccggc gctctcgacc cttcaggccc acgcccttgc accggccgcg
      961 aacgaggctg cgctcgcgat cgcagccaac gcggtcaaat ccccgatggt cggcaccgcc
     1021 tatctgcgtc cgtcgccgga atcggcgccc ttcatcacca tcggcacgca ggtcaaggcc
     1081 ggcgagaagc tgctgttgat cgaagcgatg aagactttca acgagatcgt cgcgccgcag
     1141 gctgggacgg tgacgtcgat ccttgtggag gacggacagc ccgtcgagtt tggccagcct
     1201 ctcgtcgtcg ttgaatgaga ggcgggctcg tgaacgcgca tgtttgacaa gattctgatt
     1261 gccaatcgcg gcgagatcgc actgcgcatt cttcgcgcag cgaaagaact cggcattgca
     1321 accgtcgccg tgcattcgac cgccgatgcg gaagcgatgc acgtcaagct cgcggatgaa
     1381 tcggtctgta tcgggccgcc gccggcgcgc gaatcctatc tcaatatccc cgcgctgctt
     1441 tcggcgtgcg aaatcaccgg cgccgaggcg gtgcatccgg gctatggctt cctcgccgaa
     1501 aatgcccgct ttgctgaaat cctcgaggaa catcagatcg atttcatcgg cccgagcgcc
     1561 gaacacatca gactgatggg cgacaagatc gaggccaaac gcacggcgaa gaaactcggc
     1621 atcccttgcg tgcccggctc cgatggcgcc gttgaagcgg acgaggcttt gcggatcgcc
     1681 aaggagatcg gctatccggt catcgttaag gcgtccgccg gcggtggcgg gcgcggcatg
     1741 aaggtcgcgc gttcggcgaa agaactttcc aatgcgctcg cgactgcgcg cagcgaagcc
     1801 aaggcggctt tcggcgacga cgcggtctat ctcgaaaaat tcctcaccaa tccgcgccat
     1861 atcgaagtgc agattctggg cgatggtcag ggccatgcca tccatctcgg cgaacgcgat
     1921 tgttcgctgc aacggcgcca tcagaaggtc ttcgaggaaa gtccctcccc cgctctcaat
     1981 ctggcgcagc ggcttgagat cggcgagatc tgcgccaatg ccatgcgcga gatgaattac
     2041 aaaagcgccg gcacgatcga attcctctac gacgacggca agttctactt catcgaaatg
     2101 aatacccgca tccaggtcga gcatccggta acggaaatgg tcacaggcct cgatctcgtg
     2161 aacgagcaga tcaagatcgc ggccggttcg ccgctgagca tctcgcaaag cgatgtgacg
     2221 atgctgggcc atgcgattga atgccggatc aacgcggaag atccgaagac cttccggccc
     2281 tcgccaggca agatcgccta ttatcacccg ccgggcggcc tcggcgtgcg cgtcgatagc
     2341 gccgcctatg cgggctatac gatcccgccc tattacgatt cactcatcgg caagttgatc
     2401 gtacatggcc gcacgcgtga cgaggcgatg ttgcggctgc ggcgggcgct cgatgaattc
     2461 atcgtcgatg gcattgagac gacaattccg ctgttccgcg ctttggtgcg caatgcggat
     2521 gtacaaaacg gcgaatacaa catccactgg ctcgagcatt ttctcgaaaa aggtgggatg
     2581 gacaaagcct gaggcgacct agagcgctgt gacggcatcc ttggcttgcg gcgcgtcagc
     2641 cagctcgtcg tcgggcgtgc cgacgaagcg gccaaaacgc agcgatagcg gcggcagaat
     2701 gaacagattg agcgccgtcg aggtgaagag gccgccgaga atgacaagcg ccatcgggcc
     2761 ctcgatttcg cggcccggcg tattcatgcc gatcgccaat ggcagaaggc caagcccggt
     2821 cacgagcgat gtcatcaaca ctgccgccag ccggtcgccg gcgccgtgaa ccgcagtgtc
     2881 tttatcccat gttctcgcgt cgaccgaaac cagatgctcg taatgagaaa tcatcatcat
     2941 cgaattgcgt agcgtgatgc cgaacaaagt gacgaatccg acaagtgagc cgagcgagag
     3001 caccgcgccg gaaaaataga cggcgacgac gccgcccacg agcgcaaaag gcaggttcgc
     3061 gagcgtcacg agcagattgc gccaattgcg cgtgacgacg gacaacaaaa gaacgatccc
     3121 gacgccggca aagatcgatt tcaacataag atcttgctgc gcctgcgcct gaccctccgc
     3181 cgcacctgtg aagacgatgt aggtatcggg cggcaatttg agcttgcgga gcgcggcgcg
     3241 ggcgtccttc acaaatgagg ccgggtccct gccctggacg tcgagcgtga tggattgcag
     3301 ccgccgtccg ccctgatgtt cgatcaggta aagaccagat gtcggctcga tgtcggcaac
     3361 ttgcgcaagt cgcacatagg tgccgtcggg cgcgcgaagc gtgaggtcgc caattcccgt
     3421 cacatcgccg ccgcgacgct ccgcgagccg gacgatgaca ttgaacacgc ggtcagcctc
     3481 gtaagtctga ccgacaacat caccttgata cgcggtgcgc acgacatcaa ggacattggc
     3541 gggcgcgatg tcgaagcggc gcagatcggc cgaacgaagc ctgatcgaca cctgcggcag
     3601 gcccagtggc gattccagtt cgaccgaggc cgcaccctgg actctgcgaa ggatcgttac
     3661 gacgcgctcg gcggtatcat tgatcgcgcc aagatcgctg ccgtacacgt tgaccgcaac
     3721 cggcgtcgaa tagccggaaa acgtttcatt gacgcgctcg gtcagaaacg tattcgccga
     3781 aatattgacg cccggaaaat ccaacaggtg attgaggagt gcctgtttgg cattcattgc
     3841 ggcactgccg gagatcggat taagctcgac ctcgaattcg ctctgatgcg tgccatgcgt
     3901 atcgcctgcc gccgcttgct cgacgcgccc aacgcgctgt gcgacagttt tcaccatcgg
     3961 caatttcatc atggcgtccg caatgcgcgc gcccatccgc aagccctcgg cggtcgaggt
     4021 gcctggcatt gaggtcatgt ggacgatgaa atggccttcc tgcaaatccg gcaggaaggt
     4081 ttcgccgaaa agcggcagca acgcgaggcc gccgatagtc agaacagcgg caaagaaaat
     4141 cgcgacgcgc ggcgcgccga cgatgacgcg cagaagccgc tcgtagccgc cgcgggtcca
     4201 tctgacgacc ggaggcacat gcgccttggc gtggtggggc agcagcaaca aagagagcgc
     4261 cggggtcacg gtgagcgcca cgatcagcga agcgacaacc gcataaatat aagtaagccc
     4321 aagcggggcg aagagccgcc cggcgacgcc tgacagggtc acgaccggca gaaagacgag
     4381 gagcacagcg aaggtcgcat agacaacggc gctgcgcact tcaaatgtgg cctccaagac
     4441 cacgcgcccg gccggacgcg gctgcggcaa taactcattc tcgcgcagtc tccgcgtgat
     4501 gttctcgatg ccgatcaccg catcgtccac cacctcgccg agcgcgatcg ccagaccgcc
     4561 gagcgtcatc gtattcaacg taatgcctga tcgctgcaag ataacgacgg ctgccagcag
     4621 agaaatcggg atggcggtgc agcagatggc ggcagcgcgt agatcgaaaa gaaagaggat
     4681 gagaacggcg atcaccagca caccgccgat caacagcgac gagcgaagat tgttggtcgc
     4741 ggcgatgatg aagttcgccg gccggaatag acccgtatgt agcgtgacgc cttgggcctt
     4801 cagactcggg ttgagatcgg cgagcgcagc ctcgatggcg tcggtgactt cgagtgtgtt
     4861 tgcaccatat tgctcgtcga tgttcatcac gacgccgggc tgcgccatga tcgccgccgc
     4921 gccaatggaa ggttcgggcg cttcgatcac gtcagccacg tcgccgagtg taacgcggcc
     4981 gccattgcgc agcggcacga tcgtgcgggc gatatcttcg gagtcgacgc cagatgtatc
     5041 cgtttgcaaa gtgatgcgct ggttcggagt gctgatgaag cccgcgccgc cgacgccggt
     5101 cgcgttgcgc gccgccgcga gcacatcctc catcccgagg ccataacgga tcagacgatc
     5161 tggatggaca aggatctgtg tcgacttttc ctcgccaccg aagacttcgg cgccagcaac
     5221 gccg
//
LOCUS       DASWOG010000051         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_330975,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000051 DASWOG010000000
VERSION     DASWOG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330975"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGB93_00970"
     CDS             <1..334
                     /locus_tag="VGB93_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0144732.1"
                     /translation="SSGLARFSLPGYAAYSSMKGAIEVFTRYLAKELGPRGIAANVVA
                     PGAIETDFGGGAVRDNADLNKFVASVTAMGRVGIPDDIGPMIANLLADENRWLTGQRI
                     EVSGGMFL"
     gene            complement(291..794)
                     /locus_tag="VGB93_00975"
     CDS             complement(291..794)
                     /locus_tag="VGB93_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0144733.1"
                     /translation="MVHGQEAIMLISEFVRESGLSKDTVRFYVRQGLLTPDTNGKGGR
                     NPYQVFTPEHVRVAALIRLAQSLGMSLKEIAAIGEEYARDGGISPARSIEIMNTQLSR
                     LEQKAAEIDSMARYMRAKLAWLKSGAQGPEPDIGNYIAHVVPVACAENSARSTGTSRP
                     KLRSAAR"
     gene            840..>1504
                     /locus_tag="VGB93_00980"
     CDS             840..>1504
                     /locus_tag="VGB93_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY0144734.1"
                     /translation="MNKTALVTGASAGIGEATVRRFLADGYIVYAAARRLDRMAPLAT
                     ARARLLALDLTDDASIVAAVDKIRNETGRLDVLINNAGYGSYGAVEDVPMDEARRQFE
                     VNVFGAARLCQLALPMMRAQKSGKIVNVTSVGGKFSMPFGAWYHATKFALEALSDSLR
                     LETKPFGIDVIVIEPGAIRTEWSGIARDSLITRSGETSYGPYARRLAKLFYGDATARR
                     ASPP"
BASE COUNT          281 a          463 c          461 g          299 t
ORIGIN      
        1 ttcgagcggc ctcgcccgct tctccctgcc cggctatgca gcctattctt cgatgaaggg
       61 cgcgatcgag gtgttcacgc gttatctcgc caaggaactc gggccgcgcg gcattgccgc
      121 caatgtcgtt gcgccgggcg ccatcgaaac cgatttcggc ggcggcgcgg tgcgcgacaa
      181 tgcggacctc aataagttcg ttgcctcggt cacggcaatg ggccgcgtag gcataccgga
      241 cgacatcggg ccgatgatcg ccaatctgct cgccgacgag aatcgctggc tcaccgggca
      301 gcggatcgaa gtttcgggcg ggatgttcct gtagaccgcg cgctattttc ggcgcaggcg
      361 accggcacga catgggcgat gtagttgccg atatcaggtt caggtccctg cgcgccactc
      421 ttgagccatg cgagtttggc gcgcatgtac cgtgccatcg aatcgatctc ggccgccttt
      481 tgctcaaggc gactgagctg cgtattcatg atttcgatgg accgcgctgg gctgatgccg
      541 ccgtcgcgcg cgtattcctc gccaatcgcg gcgatttctt tcagtgacat gccaagcgat
      601 tgtgccagac ggatgagcgc agcaacgcgc acatgctcgg gcgtgaacac ctgataagga
      661 ttgcggccac ccttgccgtt cgtatccggc gtcagcagcc cctgccgcac gtaaaaacgc
      721 accgtatcct tgctgagacc cgactcgcgc acgaactccg aaatcagcat tatcgcctct
      781 tgaccatgga ccatactcca cccttataga cgaggcttcc tcaaatgtgg agcaggttca
      841 tgaacaagac ggcattggtg accggcgcgt cggccggaat cggcgaagcg accgtcagac
      901 ggttcttggc ggacggatac attgtctatg cggccgcgcg tcggctcgat cgcatggcgc
      961 ctctcgcgac ggcgcgcgcg cggctactcg cgctcgattt gaccgatgac gcttccatcg
     1021 tcgccgcagt tgataaaatc cgcaacgaaa ctggccggct cgacgtgctg atcaacaatg
     1081 ccggctatgg cagctacggc gcggtggaag acgtgccgat ggacgaagcg cggcggcaat
     1141 tcgaggtcaa tgttttcggc gccgcgcggc tgtgtcagct tgcgctgccg atgatgcgag
     1201 cgcaaaagag cggcaagatc gtcaatgtaa cgtctgttgg cggcaaattc agcatgccgt
     1261 tcggcgcttg gtatcatgcg acgaaattcg cgctcgaggc gttgagcgac agcctgcgcc
     1321 tggagacgaa gccgttcggc atcgatgtga ttgtcatcga acccggcgcg atccggaccg
     1381 aatggagcgg cattgcccgc gacagtctca tcacccgctc cggtgaaacg tcttatggtc
     1441 cctacgcgcg acgcctcgcc aaacttttct atggtgatgc aacggcgcgg agggcctcac
     1501 cacc
//
LOCUS       DASWOG010000052         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147672,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000052 DASWOG010000000
VERSION     DASWOG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147672"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGB93_00985"
     CDS             complement(<1..364)
                     /locus_tag="VGB93_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144735.1"
                     /translation="MFKRKPRYRNTRLKAGRDKTILRCHLVSPATVPTNKSHSQFLII
                     IGHHMVSTYFRGHLMHQSTQPQKVQRNSRLRFTNEAKYEAERSLAHLTLIDSDRLVNL
                     LIEYYEKTDAETRTLVPLR"
     gene            251..697
                     /locus_tag="VGB93_00990"
     CDS             251..697
                     /locus_tag="VGB93_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0144736.1"
                     /translation="MRFVGRNGRRRYEVASKDRLVAACLEPGVSVSRLALEHGVNANL
                     LRKWIKKQTAPRSLPRSSPSAFIPVQLEGASDRALSRRSRVARMDLPAACDEVRGSDP
                     KGATAFCSPAKVSASLPNGVKLALECGDVDALTAIIGALGHVQTGR"
     gene            776..1030
                     /gene="tnpB"
                     /locus_tag="VGB93_00995"
     CDS             776..1030
                     /gene="tnpB"
                     /locus_tag="VGB93_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0144737.1"
                     /translation="MALDPFAPAVFAFCNRRCDRMKLLFFDRSGFVLVLKRLTEDKFR
                     WPRRQEAVVTLTTEQLHWILDGIDIDAMVRHPVRQYQVAG"
     gene            1084..1521
                     /locus_tag="VGB93_01000"
                     /pseudo
     CDS             1084..1521
                     /locus_tag="VGB93_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            1642..1995
                     /locus_tag="VGB93_01005"
     CDS             1642..1995
                     /locus_tag="VGB93_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0144738.1"
                     /translation="MIEAVAERLEGAPVRLRRRWSDELKDQAIADSLVPGANVSEIAR
                     RIGIGPSQLFDWRRKALRKGSVSLTAPQLDASPEPATSAPAFIEIVVGDIVIRADAMA
                     DEAHLRRVVRAVRSA"
     gene            1992..2336
                     /gene="tnpB"
                     /locus_tag="VGB93_01010"
     CDS             1992..2336
                     /gene="tnpB"
                     /locus_tag="VGB93_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0144739.1"
                     /translation="MIPSGVKVFLASHPIDFRKGMDGLLSLVRDAGSDPFDGALYVFR
                     AKRADRIKIVFWDGSGVVLYAKRLETAQFCWPKIGHHRVQLNQAQLMALVDGMDWKRV
                     HAVTVKPPEFVG"
     gene            2410..>2691
                     /locus_tag="VGB93_01015"
     CDS             2410..>2691
                     /locus_tag="VGB93_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0144740.1"
                     /translation="MPAPVFDLPDNVDALKAMVLAMAEKTDALKGEVAELQALNASAE
                     ERIARLTSILKTLERARFGRRSEKLGSNALDDEQSAFVFDEVQTGLGAIQ"
BASE COUNT          513 a          710 c          866 g          602 t
ORIGIN      
        1 tgcggagcgg aacgagggtg cgagtctccg cgtctgtctt ttcgtagtac tcaatcagca
       61 aatttactaa tcgatcgcta tcaatcaaag ttaggtgcgc gagcgatctc tcggcctcgt
      121 atttagcttc gttggtaaag cgtaagcgcg aatttctctg caccttttgc ggttgagtgc
      181 tctgatgcat gaggtgtcca cggaaatagg tggacaccat gtgatggccg atgatgatca
      241 gaaattgcga gtgagatttg ttggtcggaa cggtcgccgg cgatacgagg tggcatcgaa
      301 ggatcgtctt gtcgcggcct gccttgagcc tggtgtttcg gtatcgcggc ttgcgcttga
      361 acatggggtc aatgcgaacc ttcttcggaa gtggatcaag aagcagacgg cgccccgatc
      421 tctcccgcgg tcttcaccat cggcgttcat cccggttcag cttgaggggg cttccgatcg
      481 agccctgtcg cggcggagcc gcgtggcgag gatggatttg ccggcggctt gcgatgaagt
      541 gcgtgggtcg gaccccaaag gggctacagc tttttgttct cctgccaaag tgagcgcgtc
      601 gctgccgaac ggcgtgaagc tcgcgctgga atgcggcgat gtggatgcat tgacggcgat
      661 catcggagcc ctgggccatg ttcagactgg gcggtgacct gcaggtcttc tgcaccgtga
      721 gccgatcgac ttccgggctg gcatcaacag ccttgcggtt ctggtccagg agacgatggc
      781 gctcgatccg ttcgctccgg cggtttttgc gttctgcaat cgccgttgcg accggatgaa
      841 gttgcttttc ttcgaccggt ccggctttgt gctggtcctg aagcggctga ccgaggataa
      901 gttccgatgg ccgcgccgcc aagaggcggt cgtcacgctt acgaccgagc aattgcactg
      961 gatccttgac ggcattgata tcgatgcgat ggtccgccat ccggttcggc aatatcaggt
     1021 tgccggctga agaccgcaga atctgctgtt gacgtagcgg catgctttcg attcaagaat
     1081 ctgatgaatc gaaccggcgc gccgagtgtt gaagtgctgt tggcgcgcat tgctgggctg
     1141 caggcggaga accgtcagct cacagagcgc gtcgtcaagc ttgaggaaga actggcgctg
     1201 gcccgcctgc atcgttttgc gccacgcagc gaaaagcacg tcgatcgtct cttcaatgaa
     1261 gccgaacagg tcgcggacga ggatgacgcc ggcagcgaaa cgaacaatat cgtcgaactt
     1321 ccagacacgg gtttgccatc cgtcgaaagc acggctggaa agaagcgcgg ccgcagacca
     1381 ctgccggcaa acctgccgcg cgagcgcgtc gagtatgatc ttcccgacga tcagaaggcg
     1441 tgtccttgct gctgtggcca gatgcatcgc atgggtgaag ccgttaccga gcaacttcat
     1501 atcgaggtga aggcgaaggt ccgtaagcgg caaggtgatc ccatctggca tgggattttg
     1561 ggagcagtta ttgatgctgg atcacaagca ttgatgtgag cttctatgtc tgcgcctagt
     1621 tctagtgata gaagcttcca tatgatcgag gcggttgcgg aacgtcttga gggcgctccg
     1681 gttcgtctgc gccggcgatg gtcagatgag ctcaaagatc aagcgatagc cgactctctg
     1741 gtgcctggcg cgaacgtgtc ggagatcgcg cgtcgcatcg gtattggccc ttcgcagctc
     1801 ttcgattggc gtcggaaggc gctgcgtaag ggatcggtca gcctgacggc gccgcagctg
     1861 gatgcctcgc ctgaacccgc gacgtccgcg ccggcattta tcgaaatcgt tgtcggcgat
     1921 attgtcatcc gtgcggacgc catggctgat gaggcacatt tgcggcgcgt ggtgcgcgcg
     1981 gttcgctcgg catgatcccg tccggcgtca aagtttttct ggccagtcat ccgatcgatt
     2041 ttcgaaaagg catggacggt ttgttgtcgc tggtgcggga cgccggcagt gatccgttcg
     2101 acggcgctct ttatgttttt cgtgccaagc gggccgacag aatcaagatc gtcttttggg
     2161 acgggtccgg ggttgttctt tatgcgaagc gcctggagac agcgcagttc tgctggccga
     2221 agatcgggca tcatcgggtt caactcaatc aggcgcagct gatggcgctt gtcgacggca
     2281 tggattggaa gcgggttcat gcggtgacag tgaagccgcc tgagtttgtt gggtaaatcg
     2341 ctgcggcaga gtgaatcacg cggccgaaaa tgcgcgccac cggggtgaaa atatgctctg
     2401 atctgcgtca tgccggcgcc ggttttcgat ctccccgaca atgttgatgc gctcaaagcc
     2461 atggtgctgg ccatggctga gaaaacggat gctctcaagg gtgaagttgc cgaacttcaa
     2521 gccctgaatg cgagcgccga agaacggatc gcgcgcctga cctcgatcct caagacgctg
     2581 gagcgggcaa ggttcgggcg ccgctccgaa aaactcggct ccaatgcgct tgacgatgaa
     2641 cagagtgcct tcgtcttcga cgaggttcaa accgggctcg gggccatcca g
//
LOCUS       DASWOG010000053         6685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147677,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000053 DASWOG010000000
VERSION     DASWOG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147677"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1260)
                     /gene="pqqE"
                     /locus_tag="VGB93_01020"
     CDS             complement(124..1260)
                     /gene="pqqE"
                     /locus_tag="VGB93_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY0144741.1"
                     /translation="MNAPQKVSEAIAAPVGILAELTHRCPLSCPYCSNPIELDTRSGE
                     LDTETWTRVFSEAAAVGVLHVHLSGGEPASRPDLGELVKHCHDVGLYTNLITSGVGIS
                     EKRMRELADAGLDHVQLSIQDAEAESADRIGGYKGGFARKMDVAKWVNEGGIPLTVNA
                     VIHRANIARAGRMVEFAVELGARRVEIAHTQYYAWAYLNRQALMPSREQVEKAVTEVE
                     ALRKFYAGKIVIDHVAPDYYAKYPKACMGGWAKRTLNITPHGKVLPCHAAETIPGLEF
                     WNVRDHSIADIWFNAPAFNAYRGDDWMVEPCRSCPRKHQDYGGCRCQAMALTGDARNT
                     DPICHLSPNHDKVAAIDATFEEVDGNVPAGDYVYRTLKTQAPVA"
     gene            complement(1257..1544)
                     /gene="pqqD"
                     /locus_tag="VGB93_01025"
     CDS             complement(1257..1544)
                     /gene="pqqD"
                     /locus_tag="VGB93_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY0144742.1"
                     /translation="MNDAAAISADAKPHLPRGMHLKHDATRDEWLLMAPERILKLNGV
                     AVEILKRCDGNATLGEIVDQLAAAFAADRTRIDNDVRALLTELAAKRMVDL"
     gene            complement(1541..2299)
                     /gene="pqqC"
                     /locus_tag="VGB93_01030"
     CDS             complement(1541..2299)
                     /gene="pqqC"
                     /locus_tag="VGB93_01030"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY0144743.1"
                     /translation="MSLRLLSRDELEAALRAVGAERYHNKHRFHARQYSGKCTYEEVQ
                     AWALNRYYYQAMIPIKDAIVLTRLTDAEDRRQWRKRIEDHDGMQPGDGGIERWLKLTD
                     SLGFPRDYVESTAGILPATRFAVEAYATFVRERSPVEAVASSLTELFSPNIIGERMSG
                     MLANYDYVSKDTLAYFSYRPTQAKRDADWALDFVKTHAKTPEQQQAVIKALEFKCDVL
                     WTQLDALWTGYVENYRSPGVWEPGTVVTPRSRAA"
     gene            complement(2296..3195)
                     /gene="pqqB"
                     /locus_tag="VGB93_01035"
     CDS             complement(2296..3195)
                     /gene="pqqB"
                     /locus_tag="VGB93_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY0144744.1"
                     /translation="MQIIVLGSAAGGGFPQWNCLCPVCQLAWKGDKRVKPRTQSSLAV
                     SADGEHWLLLNASPDLRQQIIATPQLHPRGAKRQSPISAVFVTNGDVDHLTGLLTLRE
                     QQAFTVYGSKTTLVEVIGGNIFGVLNRELVKFNEVDLNETIDTGFGLRITPFAVPGKV
                     PLYLEGGSVEIGAEGETTIGLEITGGGKTFYYIPGCAEVNAKLQDKLRNADLLLYDGT
                     TFTDDEMVKLGLSNKTAWRMGHTAMTGEKGSLVRLSDLNIHRKIFVHINNTNPVLIED
                     SPERGKAEAAGWEISYDGMEIAL"
     gene            complement(3505..4848)
                     /locus_tag="VGB93_01040"
     CDS             complement(3505..4848)
                     /locus_tag="VGB93_01040"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY0144745.1"
                     /translation="MSAPSFERDAGQSDPILERLLDLHPKKIDLWLGRIERLLKDLGS
                     PQDRLPPVIHVAGTNGKGSSIAFMRAILEAAGKTVHVYTSPHLVRFHERIRLGDQAGG
                     RLVEDSELAETLELCEKVNDGAPITFFEVTTAAAFVLFNRHPADYLLLEVGLGGRVDA
                     TNVIARPAATLITPVSMDHPEFLGNTVAEIAGEKAGILKRGIPAVLGFQQPEALAVLG
                     AQAAKVGAPLQIAGQDFHVREEHGRLIYEDEAGLIDLPLPKLAGRHQHQNAAGAIATL
                     RLVEPALSVHALEVGLATAEWPARLQRLSKGNLVALAPAGSEVWLDGAHNEDGARVLS
                     QAMADREEKAPRPLVVVTGTLATKDTAAFLRAFKGLAQEVLAVPVHGEHAARTPADIA
                     ALANAAGLPAVACESIESALRFLTARKWAVPPRVLIAGSLYLAGEVLRLNGTPPR"
     gene            complement(4845..5711)
                     /gene="accD"
                     /locus_tag="VGB93_01045"
     CDS             complement(4845..5711)
                     /gene="accD"
                     /locus_tag="VGB93_01045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0144746.1"
                     /translation="MNWISNVVPPKIRSFLRRETPENLWVKCPDSGELIFHKDLESNL
                     YVVPGSGYHMKMPVKARLDTLFDNGAYETIPTPDVPHDPLKFRDVKRYVDRLKENRLK
                     TGAPDSVKICTGPVESIDVTVGVQDFEFLGGSLGMAAGEAIVTGMLHAVANHTPFIMF
                     TASGGARMQEGMFSLMQMPRTTVAVQKLRAAKLPYIVVLTNPTTGGVTASYAMLGDVH
                     IAEPGAVIGFAGARVIEQTIRERLPEGFQRAEYLQTHGMVDMVVRRHEIRATLARLCA
                     VLTHRPALQDAA"
     gene            complement(5786..6619)
                     /gene="trpA"
                     /locus_tag="VGB93_01050"
     CDS             complement(5786..6619)
                     /gene="trpA"
                     /locus_tag="VGB93_01050"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0144747.1"
                     /translation="MTRIDTRFAETAREGRAALVTFVMGGDPDLEASLALLKALPEAG
                     ADLIELGMPFTDPMADGPAIQAAGLRALAAKTTLAKVLALVADFREADAATPLILMGY
                     YNPIYVYGVSRFLVDAKAAGVDGLIVVDLPPEEDSELCLPALEAGIDFIRLATPTTDA
                     QRLPAVLTHTAGFVYYVSITGITGAATPDYGKVGAAVARLKQHTALPVAVGFGVKNAE
                     SAAAIAKVADGVVVGSALVDALRKSLDEEGRATSGSITAVTDLVRDLAAGIRKAAREP
                     V"
BASE COUNT         1333 a         2060 c         2004 g         1288 t
ORIGIN      
        1 atgccggacg tggcaccggt gattacaaca gttttacctt gcatgatcgc acctcggtaa
       61 agcgcgcttt caagcgaagt gagcgccggt tcgcttaaag aacgcgcaaa gtaaacccgg
      121 cggttacgcc accggggcct gcgtcttcag ggtgcgatag acatagtctc ccgccggcac
      181 atttccatcg acttcctcga aagtcgcgtc gatcgcggca actttgtcgt gattgggcga
      241 caaatgacag atcggatcgg tattgcgtgc atcgccggtc agcgccatcg cctggcagcg
      301 gcacccgccg taatcctgat gcttgcgcgg gcacgagcgg cagggctcga ccatccagtc
      361 atcaccgcga taagcgttga acgcgggcgc gttgaaccag atatcggcga tcgaatgatc
      421 gcgcacattc cagaattcga ggccgggaat ggtttcggcg gcgtggcagg gtaaaacctt
      481 gccatgcggc gtgatgttca gcgtgcgctt cgcccagcca cccatgcagg ctttgggata
      541 tttggcataa tagtcgggcg cgacatgatc gatgacgatc ttgcccgcgt agaatttgcg
      601 cagcgcctcg acctcggtca ccgccttttc gacctgctcc cgcgagggca tcagcgcctg
      661 ccggttgaga taggcccagg cataatattg cgtatgcgcg atctcgacac gccgagcacc
      721 aagctcgacc gcgaattcca ccatccggcc ggcgcgggcg atattggcgc gatggatcac
      781 ggcattgacg gtcagcggaa tgccgccctc attgacccat ttggcgacat ccatcttgcg
      841 ggcgaagccg cccttgtagc cgccgatccg gtcggccgat tcggcctcgg catcctgaat
      901 cgaaagctgg acatgatcga gccccgcatc ggccagctca cgcatccgtt tttccgagat
      961 accaacgccc gaagtgatga gattggtata aaggccgaca tcgtggcagt gtttgacgag
     1021 ttcgccgaga tcaggacgcg acgccggctc gccaccggac agatgcacat gcagaacacc
     1081 gacagccgcc gcctcggaga agacgcgcgt ccaggtttcc gtatcgagtt cgcccgaacg
     1141 cgtatcgagt tcgatcggat tcgagcaata cggacatgag agcgggcagc ggtgcgtcag
     1201 ctcggcgaga atgccgaccg gcgccgcaat cgcctccgag actttctgtg gtgcattcat
     1261 agatccacca tccgtttggc ggcgagttcc gtgagcagag cccgcacatc attgtcgatc
     1321 cgggtgcgat ccgcggcaaa cgcggcggcg agctgatcga cgatctcacc taatgtggcg
     1381 ttgccatcgc aacgcttcaa gatttcgacg gcaacgccat taagctttaa gatgcgctcc
     1441 ggtgccataa gcagccattc gtcgcgtgtt gcatcatgtt tcagatgcat accacgcggc
     1501 aaatgcggct tcgcatcagc gctgatcgct gcggcgtcgt tcacgccgcc ctgctgcgcg
     1561 gcgtcaccac cgtacccggc tcccaaacgc ccggcgaacg atagttctcg acatagcctg
     1621 tccacaaagc gtcgagctgt gtccagagca catcgcactt gaactcgagc gccttgatca
     1681 ccgcctgctg ctgctccggc gtcttggcat gtgtcttgac gaaatcgagc gcccaatcgg
     1741 cgtcgcgctt cgcctgtgtc gggcgatagg agaaataggc caaggtatcc ttgctgacgt
     1801 aatcgtaatt cgccagcatg cccgacatac gttcgccgat gatattcggc gagaataatt
     1861 cagtgagcga cgaggcaacg gcctcgaccg ggctccgctc gcggacaaag gtggcatagg
     1921 cttcaacggc gaaccgcgtc gccggcagaa tgcccgcagt cgattcaacg taatcgcgcg
     1981 gaaaaccgag gctgtcggtg agcttcagcc agcgctcgat gccgccatcg ccgggctgca
     2041 ttccgtcatg atcttcgatg cgcttgcgcc attggcggcg atcctcggca tcggtcaggc
     2101 gcgtcagcac gatcgcatcc ttgatcggga tcatcgcctg ataataatag cgattgagcg
     2161 cccaggcctg aacttcttcg taagtgcatt tgccgctgta ctggcgcgcg tgaaaccgat
     2221 gcttgttatg atagcgctcg gcgccgactg cacgcagcgc ggcttccaat tcgtcccggc
     2281 tcaaaagtct caggctcata gtgcgatctc catcccgtca taagatattt cccaccccgc
     2341 cgcctcggcc ttaccgcgct cgggcgaatc ctcaatgagg acgggattgg tattattgat
     2401 atgcacgaag atcttgcggt gtatattgag gtcgctcaac cgcacgagag aacctttttc
     2461 gcccgtcatc gccgtatggc ccatgcgcca tgcagtcttg ttggaaagac caagcttgac
     2521 catttcatca tcggtgaacg ttgtgccgtc gtagagcaga agatcggcat tgcgcaattt
     2581 gtcttgcaac ttcgcattca cttcggcgca gccgggaatg taataaaatg tcttaccgcc
     2641 gccggtgatt tcgagcccaa ttgtcgtctc gccctcggcg ccaatctcaa ccgatccacc
     2701 ttcaagatag agcggcacct tgcctggcac agcgaacggc gtaatacgca ggccgaaccc
     2761 ggtatcgatt gtctcgttga gatcaacctc gttgaatttc accagttcgc gattcaacac
     2821 gccgaaaata ttgccgccga tgacttcgac caatgtcgtc ttgctgccgt agaccgtgaa
     2881 ggcctgctgt tcacgcaggg tcaaaagccc ggtcagatga tcgacatcgc cattggtgac
     2941 gaagaccgcg gagatcggcg attggcgttt ggcgccgcgc gggtgcaatt gcggtgttgc
     3001 gatgatctgc tggcgaagat cgggcgaggc attaagtaac agccaatgtt cgccgtccgc
     3061 actcaccgcg agacttgatt gcgtccgcgg tttgacgcgt ttgtcgcctt tccaggcgag
     3121 ttggcagacg gggcaaaggc aattccattg cgggaagcct cccccggccg ccgagccgag
     3181 gacgatgatc tgcaagatag cgccctcagc tttatcgttg ggaaaccagc actggtttcg
     3241 gccgattaga tctcagccga ctcgtagctg gtgacttcca taccgacgca aacttcgacg
     3301 gcaaccggag cagtccaagc catgatttcc tccattagct tttccgtttt ggacaggtat
     3361 tcccatccaa gccggatgtg acacaaaata atgcgccagg ccacgggaag ttagggcggc
     3421 aatctctttc ttacagatgt attacaatca agcctttacg cggctttttc cggccagata
     3481 tgcaagcttt tgtgacgctg aaacctatcg cggcggcgtc ccgttgagcc gcaaaacttc
     3541 gcccgcaaga tagagcgaac cggcaatcag cacgcgcggc ggcaccgccc atttgcgcgc
     3601 tgttaaaaac cgcaacgccg actcgatgct ctcgcaggcg accgccggca aaccggctgc
     3661 attggccagc gccgcgatat cggccggcgt ccgcgccgca tgttcgccgt gaaccggcac
     3721 ggcgagcacc tcctgcgcca gccctttgaa ggcgcgcaga aaggccgcgg tgtccttcgt
     3781 cgccagtgta ccggtcacga cgacgagcgg gcgcggcgcc ttttcctcgc gatcggccat
     3841 ggcctgggag agaacacgcg cgccatcctc gttatgggca ccgtcgagcc agacttccga
     3901 gcccgccggc gccagagcga cgagattgcc cttgctcagg cgttggaggc gcgctggcca
     3961 ttccgccgtc gccagcccga cttcgagcgc gtgcaccgaa agcgcaggtt cgacaagccg
     4021 aagggtcgcg atcgcgcccg ccgcattctg atgttgatgc cggccagcca atttgggcag
     4081 cggcagatcg atcaggccag cttcgtcttc gtaaatgagg cgtccgtgtt cctcgcgtac
     4141 gtgaaaatcc tgcccggcaa tttgcagggg cgcgccgact ttcgctgcct gcgctcccag
     4201 caccgcgagc gcttccggct gctggaaacc aaggacggcg ggtatgccgc gcttcaggat
     4261 cccggctttc tcgccggcga tttcggcgac cgtattaccc aggaattcgg ggtgatccat
     4321 cgaaaccggc gtgatgagtg tcgcggccgg acgcgcaatg acattggtcg cgtcgacgcg
     4381 gccgccgaga cccacttcaa gcagtagata atccgccggg tgacgattga agaggacaaa
     4441 ggccgcggcg gtcgtcactt cgaagaaggt gatcggcgcg ccgtcattca ccttttcgca
     4501 gagctccaac gtctcagcga gttcgctatc ttcgacaagc cggccgccag cctgatcgcc
     4561 aagcctgatg cgttcgtgaa accgcacgag atgcggcgag gtatagacgt gcacggtctt
     4621 gcccgcagct tcgagaatcg cgcgcatgaa ggcaatgctc gaacccttgc cattggtacc
     4681 ggcgacgtgg atcaccggcg gcaggcgatc ttgcggcgag cccaagtcct tcaacagccg
     4741 ctctatgcgg ccgagccaca gatcgatttt cttcggatgc agatcgagca gacgttcgag
     4801 aatcggatcc gattgcccgg cgtcccgctc aaacgacggc gcgctcatgc ggcatcctgc
     4861 aaggcaggac gatgcgtgag caccgcgcaa agccgcgcca gcgtagcgcg tatctcgtgc
     4921 cgacggacga ccatatcgac catgccatgc gtctgcagat attcggcgcg ctggaatcct
     4981 tccggcagac gctcgcggat cgtctgttcg atcacccgcg cgccggcaaa gccgatgacg
     5041 gcgccgggtt cggcgatatg gacatcgccc agcatggcat aagacgccgt gacgccgccg
     5101 gtcgtcggat tggtcaacac gacaatatag gggagcttcg ctgcgcgcag cttctgcacg
     5161 gcgaccgtcg tgcgcggcat ctgcatgagc gagaacatgc cctcctgcat ccgcgcgccg
     5221 ccggaggctg tgaacatgat gaagggcgta tggttcgcaa ccgcatgcaa catgccggtg
     5281 acgatggctt cgcccgccgc catgccaagc gaaccgccaa gaaattcgaa atcctgcacg
     5341 ccaacggtga cgtcgatgct ctcgaccggg ccggtgcaga ttttcactga atcgggtgcg
     5401 ccggttttca gccggttttc cttcagccgg tcgacatagc gtttcacgtc gcgaaatttc
     5461 agtggatcat gcggcacgtc cggcgtcggt atcgtctcgt aggcgccatt gtcgaagagc
     5521 gtatcgagcc gcgccttgac cggcatcttc atgtggtagc cggagccggg aacgacatag
     5581 agattggact caaggtcctt atgaaaaatc agttcgccgc tgtccgggca tttgacccac
     5641 aggttttccg gcgtctcgcg gcgcagaaac gagcggattt tcggcgggac gacgttggaa
     5701 atccagttca tgccagtcct ttacaggggc ggcgcgcggc gccgagatca ggcagtttat
     5761 tcgtttgggg cgccgaggca aagccttaga cgggctcgcg cgcggccttg cggatgccgg
     5821 cggcaaggtc acggacgaga tcggtcacgg cggtaatgct cccggaagtc gcgcggccct
     5881 cttcgtctag agatttgcgc aatgcatcaa caagcgccga gccgacgacc acgccgtcgg
     5941 caaccttggc gatggcggca gcgctctcgg cattcttgac gccgaaaccg accgcaaccg
     6001 gcaatgccgt atgctgcttg agccgcgcga cagcggcacc gaccttgcca taatcgggcg
     6061 tcgcggcgcc ggtgatgccg gtgatcgaga cgtagtagac aaagcccgcc gtatgggtga
     6121 gcaccgccgg cagccgttgc gcgtcggtcg tcggcgtcgc gaggcggata aagtctatgc
     6181 ccgcttcgag cgccggcagg cagagttcgc tgtcttcctc cggcggcaga tcgaccacga
     6241 tcaagccatc gacacctgcc gccttcgcgt caacgaggaa acgcgagacg ccgtaaacat
     6301 agatcgggtt gtaatagccc atcagaatga gcggtgtcgc cgcatcggcc tcgcggaagt
     6361 ccgcgacgag cgcgagtact ttggcgagcg tcgtcttcgc cgccaaagcc cgtaggccgg
     6421 ccgcctggat ggcggggccg tcggccatgg gatcggtgaa gggcattccc aattcgatca
     6481 gatcggcacc ggcctcaggc agagccttca gcagagcgag cgaggcttcg agatcgggat
     6541 caccacccat gacgaaggtc acaagcgcgg cgcggccctc gcgggcagtc tcagcaaaac
     6601 gggtgtcgat acgggtcatc gcggcggtgg ctaacacgaa gccggaaaca aggaaactcc
     6661 tgcgaaagtc tcgacaaggt cacgc
//
LOCUS       DASWOG010000054         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_331040,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000054 DASWOG010000000
VERSION     DASWOG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331040"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1904)
                     /gene="ilvD"
                     /locus_tag="VGB93_01055"
     CDS             complement(60..1904)
                     /gene="ilvD"
                     /locus_tag="VGB93_01055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0144748.1"
                     /translation="MPAYRSRTTTHGRNMAGARGLWRATGMKDEDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVWPDHTV
                     KMLDLVDAMVAAADDRVSDEDVKTIERSACPTGGSCSGMFTANSMNCLTEALGLSLPG
                     NGSTLATHADRKRLFVEAGHLAVDLARRYYEQEDDKVLPRSIANFSAFENAMTLDIAM
                     GGSTNTVLHLLAAAHEGEVNFTMADIDRLSRRVPVLCKVAPAVPNVHMEDVHHAGGVF
                     GILGELDRAGLINTDTPTIHAESMGAALERWDVKRTQSRTVHDFFTAAPGGVPTQTAF
                     SQERRFDAVDLDREKGVIRSASHAFSKDGGLAVLYGNLAEDGCIVKTAGVDESILVFA
                     GPARIFESQDASVKSILGNEIVAGDIVLIRYEGPRGGPGMQEMLYPTSYLKSKGLGKS
                     CALVTDGRFSGGSSGLSIGHVSPEAAEGGLIALVKEGDRIEIDIPNRRIHLAIPDSEI
                     AERRAAMEGLELEAWKPAEPRSRKVSTALKAYASMTTSAAYGAVRRIR"
     gene            complement(2133..>2799)
                     /locus_tag="VGB93_01060"
     CDS             complement(2133..>2799)
                     /locus_tag="VGB93_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0144749.1"
                     /translation="DKCDAAAELNLAPLYAKGVGVKQDLTAAATYYRKAADRGLDSEQ
                     VALAQLYEKGRGLKQDYAEAADWYRKAAGQGSVFAQSMLGDFYVEGVGVKQDYAQAAD
                     WYRKAATQGDLFAETGLALIYAKGQGVKQDYAEASKWYAKAAARGDAFAQSNLGFLYA
                     NGQGVKQDYVQAYKWFDLAANHGPADNRATAASNRDIMADKLTAAQIKQAQTLSAGWK
                     PTL"
BASE COUNT          511 a          858 c          888 g          542 t
ORIGIN      
        1 actataggca gcgtggccgt cgcagtctgg tatccgtgtc gtgcgataag gtcacaagct
       61 cagcggatgc gacgcacggc accataggcg gcgctggtcg tcatggaggc ataggctttg
      121 agagccgtgc tgactttgcg gctccgcggc tcggcgggtt tccaggcctc caattccaga
      181 ccttccatcg cggcacggcg ctcggcgatc tcgctgtcgg gaatggcgag atgtatgcgc
      241 cgattgggaa tgtcgatctc gatccggtcg ccttccttga ccaaagcgat gaggccgcct
      301 tcggcagctt ccggcgagac gtggccgatg gagagcccgg acgatccacc agagaaccgc
      361 ccgtcagtga cgagggcgca ggatttcccc agtcctttcg acttcaggta gctcgttggg
      421 tagagcattt cctgcatgcc cggaccgccg cgcggacctt cgtaacggat gagcacgatg
      481 tcgccggcga cgatttcatt gccgaggatg ctcttcaccg acgcatcctg actttcgaag
      541 atccgagccg ggccggcaaa gacgaggatg gactcgtcga cgcccgcggt cttcacgatg
      601 cagccgtcct cggcgaggtt gccatagagc actgcgaggc cgccgtcctt tgaaaaggcg
      661 tgagacgcgg agcggatcac gcctttctcg cggtcgaggt cgaccgcatc gaaccggcgc
      721 tcctggctaa aagcggtctg cgtcggcacg ccgccggggg cggccgtgaa gaagtcatgg
      781 acggtacggc tctgcgtgcg tttgacgtcc cagcgctcaa gcgccgcgcc catcgattcg
      841 gcgtgaattg tcggcgtgtc ggtattgata aggccggcgc gatcgagttc gcccaagatg
      901 ccgaagacac cgcctgcatg atgcacgtcc tccatgtgga cgttgggaac ggcgggcgcg
      961 actttgcaga ggactgggac gcggcgcgac aggcgatcga tgtccgccat ggtgaagttg
     1021 acttcgccct cgtgcgcggc ggcgaggagg tgcaacaccg tgttggtcga gccacccatc
     1081 gcgatgtcga gcgtcatcgc attctcgaag gccgagaagt ttgcgatgga acgggggagg
     1141 actttgtcgt cctcttgctc ataatagcgc cgcgcgagat cgactgcgag atggccggcc
     1201 tcaacgaaga ggcgcttgcg gtcggcatgg gtggcgagcg tcgagccgtt gccgggcagc
     1261 gagagaccga gcgcttcggt caggcagttc atcgaattgg ccgtgaacat gcccgagcat
     1321 gaaccgccgg tggggcaggc agagcgctcg atcgtcttca catcctcgtc cgacacgcga
     1381 tcatcggcag cagcgaccat cgcgtccacg agatcgagca ttttcaccgt gtgatcaggc
     1441 catacgactt tgccggcttc catcggacca ccggacacga agacagccgg aatgttgagg
     1501 cgaagcgctg ccatcagcat accaggcgtg atcttgtcgc aattggaaat gcagaccatt
     1561 gcgtcggcgc aatgtgcgtt gaccatatat tccacggaat cggcgatgag ttcgcgcgac
     1621 ggaagcgagt agagcatgcc atcgtggccc atcgcgatgc cgtcatcgac agcgatcgtg
     1681 ttaaactcct tggcgacgcc tccggccttc tcgatctccc gcgccacgag ctggccgagg
     1741 tccttcagat ggacatgccc aggcacgaat tgtgtgaagg aattgaccac ggcgataatc
     1801 ggcttgccga aatcctcgtc cttcatacct gtcgcgcgcc agaggccacg tgcgccggcc
     1861 atgttacggc cgtgtgttgt cgttcgggag cgataggcag gcacgaatga tcctcgcagg
     1921 ttttcgcgtc tccgcgcggt atcgccggac ttttcagccc ctcgcgacga ggtccgattg
     1981 ggaagatgag ccctgaatta gcaggctgat gccaaccctg caaataggag ccggcagaac
     2041 cggcggcaag gacagacgac ccaaggccgg gtctttgagg ccggctcctg gcggagccgt
     2101 atcaggattg cctactcacg ctccaggccg cattaaagcg tcggcttcca tcctgccgac
     2161 agcgtctgcg cctgcttgat ctgcgcggcg gtcagcttat cagccatgat gtcgcgattg
     2221 ctggcggccg tcgcgcgatt gtccgccggg ccgtgattgg cggcgagatc gaaccatttg
     2281 taggcctgaa cgtaatcctg cttcacgcct tgtccgttgg cgtacaggaa gccgagattg
     2341 ctctgcgcga acgcgtcgcc gcgcgccgca gctttggcat accatttgga ggcctcggca
     2401 taatcctgct tcacgccctg gcccttggca tagatgaggg cgagcccggt ttcggcgaag
     2461 agatcgccct gcgtcgccgc cttgcgatac caatcggcgg cttgcgcata atcctgcttc
     2521 acgccaacgc cctcgacgta aaaatcgccg agcatgcttt gggcgaagac actgccttgc
     2581 ccggccgctt tgcgatacca gtcggccgcc tcggcataat cctgcttgag gccacggccc
     2641 ttctcgtaaa gttgtgcgag cgccacttgc tccgagtcga gaccccgatc ggcggccttg
     2701 cggtaataag tggccgcggc ggtcaaatcc tgcttcacgc caacaccctt ggcataaagc
     2761 ggcgcgagat tcagctcggc ggccgcgtcg cacttgtcc
//
LOCUS       DASWOG010000055         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_111190,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000055 DASWOG010000000
VERSION     DASWOG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111190"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGB93_01065"
     CDS             <1..389
                     /locus_tag="VGB93_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY0144750.1"
                     /translation="EPGDKLVCLKNDRKKGLLNGSLWNVQTAGTVRRKKIRMTVVPEE
                     DNAHKPLRIGVLPEFFTSDEEIPYALRRDSDEFDYGYALTVHKAQGSQWDNVVLFDES
                     GAFRDHRARWLYTAVTRAAETLTVVL"
     gene            414..737
                     /locus_tag="VGB93_01070"
     CDS             414..737
                     /locus_tag="VGB93_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144751.1"
                     /translation="MNDYRKIELQRLKAVVCRLMHELARQTGSDTFPLPDEDFYWKIP
                     FEEQIIENGQTKPSDFHVGRLRDDWELTQGIADYPIDQILSPYSLTEVAPLLEYMGNS
                     VTKSS"
     gene            799..1644
                     /locus_tag="VGB93_01075"
     CDS             799..1644
                     /locus_tag="VGB93_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0144752.1"
                     /translation="MKSILIPVEAHAGMSAVLETARLLARKFDSYIEGVALGPDLTQI
                     AAADFSVDGIVFDDTVRRDIFDEAHAMFETFMRESGIPLQGVPGGENAPAPSYGWARN
                     GLVSDNGVGEYGRVFDIIAVGRPGPGAQQPRKSTLESALIESGRPLLIAPPRAPSRLG
                     ETIAIAWNRSPETARTVAFALPLLKQANDVPVFTIPGYSLSGPDHVQLTESLQRHGIP
                     ARAIDVHETAKTPGLAVLVTARAAGADLLIKGGYTQSRLRQFIFGRATSQILAEANMP
                     IFMAH"
     gene            1727..2878
                     /locus_tag="VGB93_01080"
     CDS             1727..2878
                     /locus_tag="VGB93_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0144753.1"
                     /translation="MLAISKIGRRGAGLALMTLLVACAKENTFVPPPPPKVGVAYPLQ
                     QSVEPYLEATGNMTAINTVNLVARVQGFVQSIEYKDGSTVKKGTPLFVIEPVPYKLKV
                     DQAQAALDGAKAQLLQSSIEFGRQQALVSRQISTQANFDQSRANRDVNQATVEQDQAN
                     LQQAQINSGYTQVSAPFDGTVTQRLVSVGELVGTNSPTQLATIYQLDPIWVNFTISER
                     DVQTIRASMAKHGITTADVIGKVPVEVALQTDRDFPYKGVIDYIAPNVDPSTGTLNVR
                     GVLENKTFRLLPGYFVRVHVPLGKINAVLVPEEAIGADQSGRYVLVVDADNIVQQRKV
                     TIGETFGGLRRIQSGLTTKDKVIVAGLLQAAPGQKVEPQLQTLSADDAK"
     gene            2886..>4570
                     /locus_tag="VGB93_01085"
     CDS             2886..>4570
                     /locus_tag="VGB93_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0144754.1"
                     /translation="MISKFFIERPVLANVIAILTLVIGGVALFTLPIAQYPDITPPTI
                     QVTTTYPGASAKTLVETVAQPIEEQVNGVEGMIYMQSESASDGSYTLTVTFNIGTDIN
                     FAQILVQNRVQIALASLPQSVQIQGVTVQKKSTSVLSFVTLYSPDSRYNSLYLANYAT
                     IHLKDELSRVPGVGNVNVFGAGQYAMRIWLDPNKLYARALTTQDVINAVQQQNEQVTA
                     GQLGMPPVNAGQAFQYTLNVAGRLDDPHQFENIIVKTGANGAVTRIRDLGHVELGAQT
                     YSQAFSLDKQPAAGIAIYQLPGANALNVQQAVEARMKQLAKEFPEGLVYTVPFDTTTF
                     VEASVDEVYKTLFETGLLVLVVILAFLQDWRAMLVPATTVPVTIIGAFAAMAALGFSI
                     NLSTLFAIILAIGIVVDDAIVIVEGAAHHIEAGLSPHDAAIKAMQQLTGPVLGITLVL
                     MSVFLPTAFLPGLTGRMYAQFALVIAATALISAINALTLKPTQAALWLRPAKPPEQRN
                     FFYRGFNKVYARAEAGYMWIIRHLTSHGRVTVIIGLLIIVAAVYGIARIPTGFL"
BASE COUNT          946 a         1432 c         1308 g          884 t
ORIGIN      
        1 ccgaaccggg cgacaaactc gtgtgcctga aaaacgaccg caagaaaggc ctgctcaacg
       61 gctcgctctg gaatgtgcag acggcaggca ccgtccggcg caaaaagata cggatgaccg
      121 tcgtgccgga agaagacaat gcacataagc cgctgcgcat tggcgttctg cccgaattct
      181 ttacgagcga cgaggaaatc ccctacgcgt tgcgccgcga ttccgacgag ttcgattacg
      241 gctacgccct gaccgtgcat aaggcacagg gctcgcaatg ggacaatgtc gtgctcttcg
      301 acgagagcgg tgccttccgc gatcatcgcg cccgctggct ttatacggct gtcacgcgtg
      361 cggcggagac tttaaccgtc gttctctgac tgaatggagg cgtgagggtg gagatgaacg
      421 actaccgcaa gatcgagctg caacgattga aagccgttgt atgccgcctg atgcatgaac
      481 tcgcaaggca aaccggatca gacacattcc cgctgcccga tgaagacttt tattggaaaa
      541 ttccttttga agaacaaata attgagaatg gtcagacaaa gccgtcggat ttccacgttg
      601 gccgcctcag agatgactgg gaattgacgc aagggatcgc cgattaccct attgatcaaa
      661 ttttatcccc atactctctg acagaagttg cgccgctgct cgaatacatg ggcaatagtg
      721 taacgaaatc gtcttgacct ccgcccgttg tgcttcgttg ggattcgggc atcatgggcg
      781 cgcaacgcgg acgaacccat gaaatccatc ctgatcccgg tcgaggccca tgccggcatg
      841 tctgccgtgc tcgaaacggc gcggctgctc gcccgcaaat tcgacagcta tatcgaaggc
      901 gtcgcgctcg gcccggattt gacgcaaatc gccgcggcgg atttctcggt cgacggcatc
      961 gtcttcgacg atacggtccg gcgcgacatt ttcgatgaag cccacgcgat gtttgagacg
     1021 ttcatgcgcg aatcgggcat ccctctgcag ggtgtgcctg gtggcgagaa tgcgcccgca
     1081 ccgtcctatg gctgggcccg caacggcctc gtctccgaca acggcgtcgg cgaatatgga
     1141 cgtgtcttcg acattatcgc ggtcgggcgg ccgggtccgg gtgcgcaaca gccgcgcaaa
     1201 tcgacacttg aatcggcgct gatcgagagc ggtcgcccgc ttctcatcgc cccaccgcgc
     1261 gcgccgagcc gtctcggcga gaccatcgcc atcgcctgga accgcagccc ggaaacggca
     1321 cgcaccgtcg cctttgccct gccgctcttg aaacaggcca atgatgtccc ggtcttcacc
     1381 atcccgggct acagcctgag cgggcccgat catgtgcaat tgaccgaaag tctgcaacgc
     1441 cacggcatcc cggcacgcgc gatcgatgtg catgagaccg cgaagacgcc ggggctggcg
     1501 gttcttgtca cggcccgcgc cgcaggcgcc gatctcctca tcaaaggcgg ctatacgcag
     1561 agccgcctgc ggcaattcat cttcggccgc gccacaagtc agattctcgc cgaggccaat
     1621 atgccaatct tcatggcgca ttaacgcgcg ccctgcgatt gcctcgggcg ctggttcagg
     1681 gctaggttta aaaacacatt gggtcggcgc ggttcgaggg gaacgaatgc tggcgatttc
     1741 caagattggg cggcgcggcg caggccttgc gcttatgacg ctgctcgtgg cgtgcgcgaa
     1801 ggaaaatacc ttcgtgccgc cgccgccacc caaggtcggt gtcgcctatc cgctgcagca
     1861 atccgttgag ccctatcttg aagccacggg aaatatgacg gcgatcaaca ccgtcaatct
     1921 cgttgcgcgc gttcagggct tcgtgcaaag catcgaatac aaagacggct cgacggttaa
     1981 aaaaggcacg ccgctctttg tgatcgagcc ggtgccctac aagctgaagg tggatcaggc
     2041 gcaggccgcg cttgacggcg ccaaagcgca gcttctgcaa tcctcgatag agttcggccg
     2101 tcagcaggcg ctcgtctcac ggcagatctc gacgcaggca aatttcgacc aatcgcgtgc
     2161 gaaccgcgac gtcaaccagg caacggtcga acaggaccaa gccaatcttc agcaggcgca
     2221 gatcaattcc ggctatacgc aggtttcggc gccgttcgac ggcacggtga cccaacgtct
     2281 cgtctcggtc ggcgaactcg tcggcaccaa ttcgccgacc cagctcgcaa cgatctacca
     2341 gctcgacccg atctgggtga atttcaccat cagcgagcgc gacgtgcaga caatccgcgc
     2401 ctcgatggcc aagcacggca tcaccaccgc ggacgtcatt ggcaaggtgc ccgtcgaggt
     2461 cgcgttgcag accgatcgcg actttccgta caaaggcgtg atcgactata tcgcaccgaa
     2521 cgtcgatcca tcgaccggca cgctgaacgt gcgcggcgtt ctcgagaaca agactttccg
     2581 gcttctgccc ggctatttcg tccgcgtgca tgtgccgctt ggcaagatca acgccgtgct
     2641 cgtgcccgag gaagcgatcg gcgccgacca aagcggccgc tatgtcctcg tcgtggacgc
     2701 ggacaacatc gttcagcagc gaaaagtcac aatcggcgag acgttcggag gtctgcgccg
     2761 catccagtcg ggactgacca cgaaggataa agtaatcgtc gccggactgc tacaagccgc
     2821 cccggggcag aaggtcgagc cgcaattgca gacgctttcc gccgacgacg ccaaataggc
     2881 ccgcgatgat ctcgaaattc ttcatcgagc gtccggtcct cgccaatgtc atcgcgatcc
     2941 tgacgctggt gatcggtggg gtcgcgctgt tcacgctgcc catcgcgcaa tatcccgaca
     3001 ttacgccacc cacgatccag gtaacgacaa cctatccggg cgccagcgcc aagacgctgg
     3061 tcgaaaccgt ggcgcagccg atcgaggagc aggtcaacgg cgtcgagggc atgatctaca
     3121 tgcaatcgga gagcgcgtcc gatggcagct atacgctgac ggtgacgttc aatatcggca
     3181 ccgacatcaa tttcgcgcaa atcttggtgc agaaccgcgt ccagatcgcg ctcgcctcgc
     3241 tgccgcaatc cgtccagatc cagggcgtga cggtgcaaaa gaaatcgacc tccgtcctct
     3301 cattcgtcac gctctattcg cccgattcgc gttacaactc gctttacctc gccaattacg
     3361 cgacgatcca tctgaaggac gaactgtcgc gcgtgccggg cgtcggcaat gtcaatgtct
     3421 tcggtgccgg ccaatatgcg atgcgtatct ggctcgaccc caataagctc tacgcccgcg
     3481 cgctgacgac gcaggatgtc atcaatgccg tacagcaaca aaacgagcag gtgacggccg
     3541 gccagctcgg catgccgccg gtcaacgccg gccaggcgtt ccaatatacg ctcaatgttg
     3601 ccgggcgcct cgacgatccg catcaattcg agaacatcat tgtcaagacc ggcgcaaacg
     3661 gcgccgtcac acgtatccgc gacctcggcc acgtcgaact tggcgcgcaa acctacagtc
     3721 aggctttttc gctcgataaa cagccggccg cgggcatcgc catctatcaa ttgccgggcg
     3781 ccaatgcgct caacgtccag caggctgtcg aagccaggat gaagcaattg gcaaaggaat
     3841 ttcccgaggg gctcgtctac acagtgccgt tcgatacgac gaccttcgtc gaggcgtcgg
     3901 tcgatgaagt ctacaagacg ctgttcgaaa cggggcttct cgtcctcgtc gtgatcctcg
     3961 cgttcctgca ggattggcgg gcgatgctgg tgcccgcgac gaccgtgccg gtaacgatta
     4021 tcggcgcatt cgcagccatg gcggcgctgg gcttcagtat caatctctcg acgctattcg
     4081 ccatcatcct cgcaatcggc attgtggtcg acgatgctat cgtcatcgtc gaaggtgccg
     4141 cacatcatat cgaggctggg ctttcaccgc acgacgctgc gatcaaggcg atgcagcaac
     4201 tcaccggtcc ggtcctgggc atcacgctgg tgttgatgtc ggtgtttttg ccaactgcgt
     4261 tcctacctgg gctcaccggc cgcatgtatg cgcaattcgc gctggtgatc gcggccaccg
     4321 ccctaatttc cgcgatcaac gctcttactt tgaaaccaac gcaggcagcc ctctggcttc
     4381 gtccggcgaa accgccggag cagcgcaatt tcttctaccg cgggttcaac aaggtctacg
     4441 cgcgtgccga agccggttat atgtggatca tccggcatct gacctcacat ggccgcgtga
     4501 cggttatcat cgggctattg atcattgtcg cggcagtcta tggcatcgcg cgcattccga
     4561 cgggctttct
//
LOCUS       DASWOG010000056         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_221233,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000056 DASWOG010000000
VERSION     DASWOG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221233"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..877)
                     /locus_tag="VGB93_01090"
     CDS             complement(17..877)
                     /locus_tag="VGB93_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY0144755.1"
                     /translation="MDCSQDDAEMIGAKTAAQVKPAQAATPKAASLQVKAPDIGTRAG
                     RVPRHVGIIMDGNGRWASSRGLPRLEGHRRGVEALRAAVRAAIDFGLDYLTVYSFSSE
                     NWSRPVEEISDLMGLLKLFIRNDLAELGRAGVKIKVIGTRANLNPDILALLAEAETAT
                     AANTGLNLVVAFNYGSRQEIAEAARLIAEKVAAGTLDSASINAEVVAQHLDTAGIPDP
                     DLIIRTSGEQRLSNFLMWQAAYSEFVFLPIHWPDFDRAALAAALDEFAARERRFGGVT
                     LSPASAKSGR"
     gene            complement(983..1543)
                     /gene="frr"
                     /locus_tag="VGB93_01095"
     CDS             complement(983..1543)
                     /gene="frr"
                     /locus_tag="VGB93_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0144756.1"
                     /translation="MSDGFDLSDLKRRMQGSIVSLKHELGGLRTGRASASLIEPIHVD
                     VYGQSMPINQVATISVPESRMLSVQVWDKGVVGAVERAIRDANLGLSPVTEGQTLRIR
                     IPELNEQRRKEMVKVAHRYAEEARVAVRHVRRDGIDHLKRLLKDKLISEDDEKRHATD
                     VQKVTDQHVGEIDVALATKEQEIMQI"
     gene            complement(1731..2453)
                     /gene="pyrH"
                     /locus_tag="VGB93_01100"
     CDS             complement(1731..2453)
                     /gene="pyrH"
                     /locus_tag="VGB93_01100"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0144757.1"
                     /translation="MTDSKPKWRRIVVKLSGEALMGQASHGLDARTVEAIAADLAGAA
                     RLGVETGVVVGGGNFFRGIAGAETGIERARADSIGMLATVMNALAMEHAIEKQGQPAR
                     ALSAVAMPSLCEPYSRQAALHHLGKGRVVVLAGGTGNPFFTTDTGAALRAAELSADVI
                     LKATQVDGVYSADPKLDLNAQRYETLTHDEAIAKNLAIMDTAAFALARETKIPILVFS
                     IKEPGAICAVLKGVGRATLVTP"
     gene            2844..5282
                     /locus_tag="VGB93_01105"
     CDS             2844..5282
                     /locus_tag="VGB93_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0144758.1"
                     /translation="MLLISKRSRVPFASAFLIALSSTVFYAPHAAAQQAASGELPTVD
                     VVATTPLGASTSTLDVPSETQTLTSEEISTVNQTTLQDALARRTPGVSVTDEIGSPLS
                     ESVDFRGETATPVPGTPEGLAVYMNGVRINESYGDVVNWDLIPPSAINTAQIVTGNPV
                     FGLNALAGAVVIQMKNGFNWQGTEVDLQGGMDYTAQGSAQYGVNKGNWAYYVDVDGVR
                     TNGFRYFGQSDAERAYGDIGYRAWNSELHLSMTGGADGLGVAGTTPLELANENPSAVF
                     TTPQTTNTTAEMITLSDETHITPTLTFNGNAYFRSYAQAHHDGNISDFYHCTASSGEA
                     GDHVCNGGDLVPDLADPLAGTGAPDGQPLGEIDSNWTRTLSTGATGQLTDTDKIFGHN
                     NTITAGVSVDNGWTHFTGSSTLGTLPANFVVPFTNESINEPDDDISPADVRTQNTYIG
                     IYVLDNFDITDRLSVHAGARFNDAIISLTDESGENPDLNGSHNFNRINPVVGATFKIT
                     PDISVYASYSEANRAPTPLELGCSSPDHPCQIDNFLVADPPLQQIVARTVEAGFKGSN
                     AINWSWAPGRLDWSIAGYHTENQNDIYSVPSLVTGFGSFTNAGDTLREGVDVGATYTT
                     ARWDVYANYSYIQAKFLTPVLLSSPNNPFADGNGNIQVESGDNLPGIPTNKFKFGIDY
                     EVLPGWKVGGDVVYRSSQYYFGDEINALPKIPGFATLDLRTSYQVNKDVQVFGLINNA
                     LNFRGATYGSLYETDSTQNQVNGAAPGVCDAGLFCSADPRAITIAPPFEAMVGLKMTL
                     NDAPPPSPPLVAKY"
     gene            5356..>5714
                     /locus_tag="VGB93_01110"
     CDS             5356..>5714
                     /locus_tag="VGB93_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144759.1"
                     /translation="MTSRRAPRRLVYLYRDAIAFFFTSDTHFGDARRIRVDKRPFKSI
                     GEHVAALIARWNAVVSPEDDVYHLGDFAVTMDATAVESWLAALNGRKHLIIGNNDSPA
                     VTASKTWASISHYAELV"
BASE COUNT         1161 a         1796 c         1599 g         1158 t
ORIGIN      
        1 gcctctttag aataggtcat cggcccgatt tagcactggc cggtgataag gtcacgccgc
       61 cgaaacggcg ctcgcgggcg gcaaactcgt cgagcgccgc cgccagcgcc gcgcggtcaa
      121 agtccggcca atggatcggc agaaagacga attcgctgta cgccgcctgc cacatcagga
      181 aattcgacaa gcgctgctcg cccgaggtgc ggatgatgag gtcggggtct ggaattcccg
      241 cagtatcgag atgttgcgcg acgacctcag cattgatcga tgcggaatcg agcgtgcccg
      301 cggcaacttt ctcggcgatg agccgcgctg cctcggcgat ttcctggcgg ctgccgtagt
      361 tgaacgcgac gacaagattg agacccgtat tcgccgccgt cgcggtttca gcttctgcaa
      421 gcagagccag gatatccgga ttgagattgg cgcgtgtccc aatcaccttg atcttcacgc
      481 ccgcacggcc aagttcggcg agatcattgc ggatgaagag cttcaaaagc cccataagat
      541 cgctgatctc ttcgacgggc cggctccagt tctccgacga aaagctgtaa accgtcaggt
      601 aatcgaggcc gaaatcgatc gccgcacgca ccgccgcgcg caaagcctcg acaccgcgcc
      661 ggtgtccttc aaggcgtggc aagccacggc tcgacgccca acggccgttg ccgtccatga
      721 tgatgccgac atgccggggg acgcggccag cccgtgttcc gatgtccggc gccttgactt
      781 gcagagacgc cgctttcggg gtcgccgcct gggccggctt gacttgcgcc gcagtcttcg
      841 ccccgatcat ctccgcatca tcctggctgc aatccattgt gttttatcga tatgcgcggg
      901 tatcagaacc gcgccgcatt tccgatctta agggcagaaa tcctttttat gtccaaacgt
      961 gccgcgcccg ccaaatcagc cgctaaatct gcatgatttc ttgttctttt gtcgcaagcg
     1021 cgacatcgat ctcgccgacg tgctgatcgg tgactttctg cacgtccgtg gcatgacgct
     1081 tttcatcgtc ttcgctgatc agcttgtctt tcaaaaggcg cttcagatgg tcgatgccgt
     1141 cacggcggac atggcgcacc gcgacccgcg cctcttccgc gtagcggtgc gcgactttca
     1201 ccatttcctt gcggcgctgc tcattgagtt ccggaatccg gatccgcagc gtttggcctt
     1261 ccgttaccgg cgacaagcca agattcgcat cacgaatggc gcgctcgaca gcgccgacga
     1321 cacccttgtc ccagacctgc accgacagca tccgcgattc cggcacgctg atcgtcgcga
     1381 cctgattgat cggcatcgac tgaccataga catcgacgtg aatgggctcg atgaggcttg
     1441 ccgaagcgcg acccgtccgc aggccgccga gttcatgctt cagcgacacg atcgacccct
     1501 gcatgcggcg cttcaaatca gaaagatcga acccgtcact catcttgaac cttgcctgag
     1561 tgaaatttcg ccgccggccg gcgcgccggc attccggcgc atcctcgcct tcagcacctc
     1621 taatacggcg tctttccggc tagatttggc gatggcggcg gatatgatca cagaatcttt
     1681 cgctttgcta tgccatttca agggctccgc caagcccacg ttcaagcccg ctaaggcgta
     1741 acgagggttg cgcgcccgac gccctttaga acggcgcaaa tggcccccgg ctccttgatc
     1801 gaaaacacca aaatcggaat tttcgtctct cgtgccagcg caaacgcggc ggtgtccatg
     1861 atcgcgagat tcttggcgat ggcctcgtca tgcgtcagcg tttcatagcg ctgcgcgttg
     1921 agatcaagct tcggatcggc ggaatagacg ccgtccacct gcgtcgcctt gagaatcaca
     1981 tctgcggaca gctccgccgc ccgcaatgcc gcgccagtgt cggtcgtaaa gaacggattg
     2041 cccgtgccgc ccgcgagcac gacgacgcgg cccttgccga gatgatgcag cgccgcctgg
     2101 cgcgaatagg gctcgcacag cgagggcatg gcaacagctg acagcgcacg cgccggttgg
     2161 ccctgctttt caatcgcatg ttccatcgca agtgcgttca tgacggtcgc caacatgccg
     2221 atcgaatcgg cgcgggcgcg ctcgatgccg gtttctgcgc cggcgatgcc gcgaaagaaa
     2281 ttgccaccac cgacgacgac gcctgtctcg acgccgagcc gggccgcacc cgcgagatcg
     2341 gcggcgatcg cctcgaccgt tcgcgcatca aggccgtgac tcgcctgccc catcagggcc
     2401 tcaccagaga gtttgacgac gatccgccgc catttcggtt tgctatcggt catggatcgt
     2461 ccatctgctg gttttcgcgg cgtctttcta acgtaatgca gcttcatccc gaagccgaaa
     2521 aaccacggat ttcaccacac tttcaaaatg gacgggaagt tatgcgcaga gaatgcgcgg
     2581 ataccgtaag gcagatgggc aggctaaaga acccttcctg ttttgccaat tttgcttgct
     2641 tttttatccc gataatattg agaacatttc ccgcgatgat cggatttttc cgtgcaattc
     2701 caaagtttta ggaagtctct gttgccaact tgcactagag agactgccga tcatgcccta
     2761 ttgttacaga tatagtgaaa gacgaatcgg gcgaaatcgg caaaagccgg cgaccgaatc
     2821 ttgagcatat ccggggggga ctgatgctgc taatttccaa gcgttctcgc gttccgtttg
     2881 catcggcgtt tttgatcgcc ctgtcttcca cggttttcta tgccccacat gccgccgcac
     2941 agcaggcggc gagtggcgag cttccgacgg tcgatgtcgt tgcaacgacg cccctgggcg
     3001 ccagcacgag cacgctggat gtgccgagcg aaacgcagac gcttacgagc gaagaaatca
     3061 gtacggtgaa tcaaacgacg ctgcaggatg cgttggcacg tcggacacct ggcgtttcgg
     3121 tgaccgacga gatcggtagc ccgctctccg aatcggtcga cttccgcggc gagactgcga
     3181 cccccgtccc gggaacgcct gagggcttgg ccgtctacat gaacggcgtc cgaatcaacg
     3241 aatcctacgg cgacgtcgtc aattgggatc tgatcccgcc atccgcgatc aacacagcac
     3301 agatcgtcac cggcaatccg gtcttcggcc tcaatgcgct cgccggcgcg gtggtcattc
     3361 aaatgaagaa cggcttcaac tggcagggca ccgaagtcga tctccagggt ggcatggatt
     3421 ataccgccca gggttcggcc caatacggcg tcaacaaagg caattgggcc tattacgtcg
     3481 acgtcgatgg cgttcgcacc aatggcttcc gctatttcgg ccaatcggat gccgagcgcg
     3541 cctatggcga catcggctac cgcgcctgga acagcgaact ccatttgagc atgacaggtg
     3601 gggccgatgg cctcggagtc gcaggcacga cgccgctcga actcgccaat gaaaatccgt
     3661 ccgccgtctt cacgacgccg cagacaacca atacgacggc ggagatgatc acgctgtcgg
     3721 atgaaacgca tatcaccccg acgctgacgt tcaacggcaa cgcttatttc cgcagctatg
     3781 cccaggccca tcacgacggc aacatcagcg atttctacca ctgcacagcc tcctccgggg
     3841 aggcaggcga tcacgtctgt aacggtggcg atttggtccc tgaccttgcc gacccgctcg
     3901 cgggaaccgg cgctcctgac ggccagccct tgggtgagat cgacagcaac tggacgcgaa
     3961 cattgagcac cggcgcaacc gggcagctca ccgacaccga caaaatcttc ggtcataaca
     4021 atacgatcac cgcgggcgtc agcgttgaca atggctggac gcatttcacc ggcagcagca
     4081 cgctgggtac attgcctgct aatttcgtcg ttccgttcac gaacgaatcc atcaacgagc
     4141 cggacgacga catttcgccc gcggatgtca gaacgcagaa cacctatatc ggcatctatg
     4201 tgctcgacaa tttcgacatc accgacagat tgagcgtcca tgccggcgcg cggttcaacg
     4261 atgcgatcat ctcattgacc gacgagtcgg gcgagaaccc ggacctgaac ggctcgcata
     4321 atttcaatcg tatcaaccca gtcgtcggtg cgacgtttaa gatcacgccg gatatttcgg
     4381 tctatgcgag ctattcggaa gccaaccgtg ccccgacgcc gctcgaactc ggctgctcca
     4441 gcccggatca cccttgtcag atcgacaatt tcctcgtcgc cgatccgccg ttgcaacaga
     4501 tcgtcgcgcg cactgtcgaa gcgggcttca agggcagcaa tgcaatcaac tggtcatggg
     4561 cgcccggccg gcttgactgg agtatcgccg gctaccacac tgagaatcag aacgatattt
     4621 acagcgttcc gagccttgtc acgggctttg gctctttcac caatgccggc gatacgctgc
     4681 gcgagggcgt cgatgtcggc gccacttata cgacggcccg ttgggacgtc tacgccaact
     4741 attcctacat tcaggcgaag ttcctgacgc cggtgctgtt gtcctcgccg aacaatccct
     4801 tcgccgatgg taacggcaat attcaggtgg aatcgggcga caatctcccg ggtatcccga
     4861 ccaataagtt caagttcggc atcgattacg aggtcctgcc aggttggaag gtcggtggcg
     4921 atgtcgtcta tcgctcaagc cagtattatt ttggcgacga gatcaacgcg ctgccgaaaa
     4981 ttcctggctt cgcaacgctc gatctgcgga cctcgtacca agtcaacaaa gacgtccagg
     5041 tctttggtct gatcaacaac gcgcttaact tccgcggcgc gacctacggg tcgctgtacg
     5101 agaccgattc gacccagaac caggtgaacg gcgctgcccc cggcgtttgc gacgccggtc
     5161 tgttctgctc cgcggacccc cgtgcgatca cgatcgcccc gccgttcgaa gcgatggtcg
     5221 ggctgaagat gacgctgaac gacgcgccgc caccgtcccc gccgctcgtg gccaaatact
     5281 gagccgcgca attggacttt aggactggcg gacaacccgc cagtctttct ttaagacgcc
     5341 cgatgacggg ttaaattgac gagccgccgc gcaccgcggc ggctcgttta cttatatcgg
     5401 gacgcgatcg ctttcttctt caccagcgac acacatttcg gcgacgcccg ccgtattcgc
     5461 gtcgacaagc gcccgttcaa gtcgatcggc gagcatgttg ctgcgctgat tgcgcgctgg
     5521 aatgccgtgg tcagccccga ggatgacgtt tatcatctcg gcgatttcgc ggtgacaatg
     5581 gacgcaacgg cggtcgaatc gtggcttgca gcgctgaacg gccgcaaaca tctcatcatt
     5641 ggcaataacg attcgcccgc ggtgactgcc agcaagacgt gggcgagcat ctcacattac
     5701 gccgaactcg taat
//
LOCUS       DASWOG010000057         9165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_331127,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000057 DASWOG010000000
VERSION     DASWOG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331127"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..619)
                     /locus_tag="VGB93_01115"
     CDS             complement(17..619)
                     /locus_tag="VGB93_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0144760.1"
                     /translation="MDLNSPLFDRVRAKPKAKQPVQAAPKARCDHPGCNEPGEFRAPM
                     GWQRENEYFTFCLEHVRAYNATYNYFQGMSAEAVALYQRDASTGHRPTWVMGNNGAGG
                     PQAHGDPSQYADPLNIFGTRAAPHKPAEPPKPKHGIAARKALDQLGLDETADATTIRS
                     RYKDMVKRLHPDANGGDRSLEDKLREIIRAYNYLKSVKLA"
     gene            709..1008
                     /locus_tag="VGB93_01120"
     CDS             709..1008
                     /locus_tag="VGB93_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0144761.1"
                     /translation="MSNEPNIAERIKQKLQAGLAPVSLAVVDDSHRHAGHAHAIEHAA
                     KTGKVGETHFKVKVVSESFRGKGLVTRHRMVNDLLQDEFADGVHALSIDAKTPGD"
     gene            1177..2685
                     /locus_tag="VGB93_01125"
     CDS             1177..2685
                     /locus_tag="VGB93_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0144762.1"
                     /translation="MRLRFHGLQTHGLIAAAVLMIVAGGALATPSLVMDVATGNVLYE
                     NEATHPWYPASVTKLMTVYVALSAVRDHQISLDTPLVVSRYAASMPPSKMGFRPGTEV
                     TLGNALKMLMVKSPNDIAITIAEGIAGSVPAFAEDMNASAAQLGMRQSHFVNPNGLHN
                     PEHYSSARDLAVLARAIYLTFPQYADLFDIGAMRVDGRIIPNHNDMLGRYPGADGMKT
                     GFTCDSGFNLVASATRGNHHYLAVILGAPTPRERMIRTAVLLDRAFAGIDQPQPMDAD
                     EPTAGAPEDMRDLVCHRRGRAMTAFQAETDRLEAPLLTLGQKSSPLGVFTASALAEET
                     PAALRIAMMPVPVFDPVTLTTGPAAGYSGPIAGPRPPHTPIGTPLLADTAPSTPPAAN
                     GPDEDSPEAGAAHHVGAKVAASDEGDADPPAPPSKHQKITKAKSHKKVVKAAARGYSK
                     HKAVKGKAAKSKSGKLAKAKVKSKAAKPAAKKAKAKKAAGKSHGEHSSHASH"
     gene            2794..3960
                     /locus_tag="VGB93_01130"
     CDS             2794..3960
                     /locus_tag="VGB93_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0144763.1"
                     /translation="MPDPRRPPIFPLTILTGFLGAGKTTLLNHLLRAPELSDSLVLIN
                     EFGEVGLDHLFVEKVDGNVVVMASGCLCCTIRGDLVSTLEDLLRKRDNNRVVPFNRVI
                     IETTGLADPAPVLHTIMYHPYFMLRFRLDGVITLVDALNGAATLDRHPEAVKQAAVAD
                     RLVISKSDLLSEDDVGLAALHSRLKALNPTAIQLDAARGEATPAVLFHIGLYDPDKKT
                     PDVRRWLNAEALDDHAHAHHDDHDHHGHHDHSDHDHAHPHDVNRHDARIRAFALRHPT
                     PVPPAMVDMFLELLRSAHGAHLLRVKGIVALDDDPACPLVIHGVQHIFHPPVRLEAWP
                     DDDHTTRIVFILYDMEPSFIQGMWNAFVGVAADTPDAAALTQNPLRVSSGGLLA"
     gene            complement(3957..6491)
                     /locus_tag="VGB93_01135"
     CDS             complement(3957..6491)
                     /locus_tag="VGB93_01135"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEY0144764.1"
                     /translation="MNFLRAIVRSFAVAALLLCMGIAAQAQTFIAINSANLDASANTL
                     QQVETALQQPGLSKDVLQKLQEQTAPLGPTLQRVLDHLTPHLAAQKAQLDQLGAPPDA
                     KAPPESKEVAAERVKRQKAFDDVDALVKRTNLLIVQVDQAQTTIASRLRARLTEALLQ
                     QEPSIASPSLWADVVREVPRNLRALRHRTGGWLSDSAAKLTGWRASLFCGLLVLIGIL
                     YWPVTQFAQRMFFRKDVADNPTPLQKIVAAWRVLFVIAAIPIATALLIGFLARSFGLV
                     DDQNPLLRPLFFSVVRIAIGAGLAQALLAPKREQWRLIAVDSDAASQVLRTVLIVTVL
                     VSCGRIFNAVGSAIEASWSSDMFARGFFSLLAAIAIAASLWIGQSGDEEESEQVFGPR
                     VDQTANLFSILRTVLWAAILVVFAAVLAGYMSLGRFAIDQIIWVGGLGGLAVMLVMLI
                     DQLVGQGITGTTPFGRRMMLSTGLSREAFAQFAILASGVARVVIFLIAVLAALVPWGV
                     QSADVTGYLYAAFFGFRIGDVTISLSRIVFTLVIFAAGYAATSAVERWLEVSFLPHTR
                     LDLGLRNAIKTSIGYIGRIFALGFALAYLGVDFAKLAIVAGALSVGIGFGLQSIVNNF
                     VSGLILLWERAIRVGDWISVGANEGFVRRINVRSTEIETFDRAAVVIPNSDLVAGVVK
                     NFVRTDKTGRLKLNIPVNAAADPQKAREVLLDVTKSNKNVLKKPTPFVVFAEITGGVF
                     NFELYCYVSDVTSMKSITSELNFEIYRRFKDANLFDAPPPVMNINLPELEKRGFILRE
                     QVDRNPANGKETSTDEGEADTHSREENASKQKKQSLGSDPIRTKKL"
     gene            complement(6566..7609)
                     /locus_tag="VGB93_01140"
     CDS             complement(6566..7609)
                     /locus_tag="VGB93_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEY0144765.1"
                     /translation="MSHATLTSVDDLIAQGFAPPAARESLMRVGDLYAIAINETMAAL
                     IDRNDPADPIARQFIPDAAELITTPEELADPIGDTPMSPIEGVVHRYPDRVLLKLLHV
                     CPVYCRFCFRRESVGPGGATHLSSEALDKAFAYIAAHDEIFEVILTGGDPLILSLRRL
                     EEIFARLRATTHVQVVRIHTRVPVVGPELISDALVDLLRRSGKTIYVALHANHARELT
                     PQAQTACAKLIDAGIPMLSQSVLLRGVNDDVETLAALMRAFVAARIKPYYLHHLDLAR
                     GTSHFRLSVAEGQKLVEELRGKLSGLCQPRYMLDIPGGFGKVTLDGTAPDEDGRYHLR
                     DFRGLTHLYPPAT"
     gene            complement(7606..8658)
                     /gene="epmA"
                     /locus_tag="VGB93_01145"
     CDS             complement(7606..8658)
                     /gene="epmA"
                     /locus_tag="VGB93_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY0144766.1"
                     /translation="MSKPSFWWDPAVHAEKRRFLTARAALAKAARGFFDARGFIEADT
                     AILQISPGNEMHISAFATEMIAPDDARSRLYLHSSPEFALKKLLAAGEEKIFSLGHVF
                     RNRERGPLHPPEFTMLEWYRTNAPYTALFADCRELLAVAATAAQTDTFRFRDATADAF
                     AEPEILTVADAFQRYAGVDLLQTYDAQSCDRKALADRTAALGLRVAVDDTWSDLFSKI
                     LSAKIEPNLGHGRATLLVDYPAPEAALARLKHDTRFAERFELYVCGVELANGFGELTD
                     ANEQRARFIGDMIARERIYSDAYPIDEDFLAAIAQMPPASGIALGFDRLVMLATGARR
                     IEDVLWTPIADSGTAR"
     gene            8852..>9165
                     /locus_tag="VGB93_01150"
     CDS             8852..>9165
                     /locus_tag="VGB93_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0144767.1"
                     /translation="MKVIASSIRKGNILERDDGQLYVVLTAESFHPGKGTPTTQIDMR
                     RLSDGVKTTDRYKTTEQVERAFVEDHDYSYLFQDGEHYTFMNGETYDQVNVSADVIGE
                     QAV"
BASE COUNT         1867 a         2773 c         2816 g         1709 t
ORIGIN      
        1 acggcggacg ctgaaactaa gcgagtttaa ccgatttcag atagttgtag gcgcgaatga
       61 tttcgcgcaa tttgtcttcg agcgaacggt cgccgccgtt cgcatcgggg tgcaggcgct
      121 tcaccatgtc cttgtaacgg ctgcgaattg tcgtcgcatc cgcggtttcg tcgaggccga
      181 gctgatcgag cgctttgcgc gcagcaatgc catgcttggg cttcggcggc tccgccggtt
      241 tatgcggcgc ggcccgcgtg ccaaaaatat tgagcgggtc ggcatattga gaggggtcgc
      301 cgtgcgcttg cgggccaccc gcgccattgt tgcccatgac ccaggtcggc cgatgtccgg
      361 tggaggcgtc acgctggtag agcgccacgg cctcggcgct cataccctga aagtaattgt
      421 aggtcgcgtt ataggcacgc acatgctcaa ggcagaacgt gaaatattcg ttttcgcgct
      481 gccaccccat cggcgcgcga aactcgcccg gctcgttgca gccgggatgg tcgcagcgcg
      541 ccttgggggc ggcctgcacc ggctgcttcg ccttcggctt ggccctgaca cgatcgaaga
      601 gaggtgaatt caaatccatg gcgcgataat acgaatgtct gtgcggtggc cgcaagccgc
      661 cgcgtccggt aaaagacaaa ggcgggctgg cgatgaagga aagtttcgat gagcaacgag
      721 cctaatatag ccgagcgcat caagcaaaag ttacaggccg gtctggcgcc ggtttcgctc
      781 gccgtggttg acgattcgca ccgtcacgcc ggacacgcac atgccatcga acacgcggcc
      841 aagaccggca aagtgggcga gacccacttt aaagtcaaag tggtgtctga aagctttcgc
      901 ggcaagggcc tggtgacgcg ccaccgtatg gtcaacgacc tgctgcaaga cgaattcgcc
      961 gatggcgtgc atgccctgtc gatcgacgcc aagacgcccg gcgattaggg ctttcaaacc
     1021 gaggccatcg tcatgaaatg gccgcatctt ccacgaagta agaacggtac cgaaagtcac
     1081 cggcagcttt gcagattgtt gccaaagcgt tagacttgtg gcgggcgtcg aaaaaaccgg
     1141 caaatcttca aggcgagttt ttcagggcaa gtgccgatgc ggttgcgttt tcatggcttg
     1201 caaactcatg gcttgattgc cgctgcggtc ttgatgatcg tggccggggg cgccctggca
     1261 acgccgtcgc tcgtcatgga tgtcgcgacc ggcaacgtgc tttatgaaaa tgaagcgacg
     1321 cacccctggt atcccgcgtc tgtcacgaag ctcatgacgg tttacgtcgc gctctccgct
     1381 gtgcgcgatc atcagatttc gctcgacact ccgctcgtcg tatcgcgcta cgcagcgtcg
     1441 atgccgccct cgaagatggg cttccggccc ggcaccgaag taacgctcgg caacgcgctc
     1501 aaaatgctga tggtgaaatc gccgaacgat atcgcgatta ccattgccga aggcattgcc
     1561 ggctcggtac cggcctttgc cgaagacatg aacgccagcg ccgcgcaact cggcatgagg
     1621 cagagtcatt tcgtcaatcc gaacggtctg cataatccag aacattattc gtcggcgcgc
     1681 gacctcgctg ttctggcgcg ggcgatctat ctcacgtttc cgcaatatgc cgatctcttc
     1741 gacatcggcg ccatgcgtgt cgacggcagg atcatcccga atcacaacga tatgctcggc
     1801 cgctatcccg gtgccgacgg tatgaagacc ggcttcacct gcgattccgg cttcaacctt
     1861 gtcgccagtg cgacgcgcgg caatcatcat tacctcgccg tcatcctcgg cgcgccgacg
     1921 ccgcgcgaac gcatgatccg caccgccgta ctcctcgacc gcgccttcgc gggaatcgac
     1981 caaccgcagc ctatggatgc cgacgagccc accgccggag cgccggaaga tatgcgggat
     2041 ctcgtctgtc atcgccgcgg ccgtgccatg acggcgttcc aagcggaaac cgaccgcttg
     2101 gaggcgccgc tactgacact cggccagaaa tcgtcgccgc ttggcgtctt cacggcgtcg
     2161 gccctggcgg aggagacgcc ggccgctttg cggattgcga tgatgccggt gccggtcttc
     2221 gatcccgtta cgctcacgac cggtccggcc gccggctaca gcggcccgat tgcgggcccg
     2281 cgtccgccgc acacgccaat cggtacgccg cttctcgccg acaccgcgcc cagcacgccg
     2341 ccggccgcaa acggacccga cgaagattcg ccggaagccg gggccgccca tcatgtcggg
     2401 gcgaaagtcg cagcgagtga cgaaggcgat gcagatccgc cagctccgcc gagcaaacat
     2461 cagaaaatca ccaaggccaa atcgcataag aaggtcgtga aagctgcggc gcgagggtat
     2521 agcaagcaca aagcagtaaa aggcaaagcc gcgaaatcga agtccggcaa acttgccaag
     2581 gcaaaagtaa agagcaaagc ggcgaaaccg gcggccaaaa aggccaaggc aaagaaggcc
     2641 gctggcaaga gccacgggga acattcgtcg cacgcaagcc attgaggctt gcagcgcggt
     2701 ccgattgaat cggagcggcc cccggcttat ctcttgccag cacgatcttt tgcccggacg
     2761 catggttcgc gggatcgcgc tggattttaa gagatgcctg acccgcgccg gccgcccatc
     2821 tttccactga cgattctcac cggctttctc ggcgccggca agacaacgct gctcaatcac
     2881 ttgctgcgcg cgccggaact ttcggacagc ctcgtgctga tcaatgaatt cggcgaggtc
     2941 ggactcgatc atctcttcgt cgagaaggtt gacggcaatg tcgtcgtcat ggcctccggg
     3001 tgtctttgct gcacaatccg cggcgatctc gtctcgacgt tggaagatct gctacgcaag
     3061 cgcgacaaca atcgcgtcgt gccgttcaat cgcgtcatta tcgagacgac cggccttgcc
     3121 gatccggcgc cggtgctgca tacgatcatg taccatccct atttcatgct gcggtttcgc
     3181 ctcgacggcg tcatcaccct tgtcgatgca ttgaacggcg cggcgacgct cgaccggcat
     3241 cctgaagccg tcaaacaggc cgccgttgcc gatcggctcg tgatcagcaa gagcgatctt
     3301 ctgtctgagg acgatgttgg ccttgcggca ttgcatagcc gcctcaaggc gctcaatcca
     3361 accgcgattc agcttgacgc cgcgcgcggc gaagcgacgc cggccgtgct gttccacatc
     3421 ggtctttatg atccagacaa gaaaaccccg gatgtgcgcc gctggctgaa tgccgaagcg
     3481 ctggatgatc acgcgcatgc tcaccatgac gaccatgatc atcacgggca tcatgatcat
     3541 tccgaccacg atcatgcgca cccgcatgac gtcaaccggc acgatgcgcg aatccgcgcc
     3601 ttcgcgctac gccatccgac gccggtcccg ccggccatgg tcgatatgtt tctcgaattg
     3661 ctgcgcagcg cgcatggcgc gcatctcctg cgcgtgaaag gcatcgtggc gctcgatgac
     3721 gatccggcgt gcccgctcgt cattcatggc gtgcagcata ttttccatcc gccagtgcgg
     3781 ctcgaggcgt ggcccgacga tgaccatacg acgcgcatcg tcttcatcct gtacgatatg
     3841 gaaccgagct tcattcaagg catgtggaac gcttttgtcg gcgtcgcggc cgacacaccc
     3901 gatgccgcgg cgctgacgca aaatccgctg agagtttcgt cgggcggcct gctggcttag
     3961 agttttttgg ttctgatggg atcagaacca aggctctgtt ttttttgctt tgacgcgttt
     4021 tcttcacgcg aatgggtatc cgcttcgccc tcgtccgttg atgtctcctt gccatttgcg
     4081 ggattgcgat ccacctgctc gcgcaggatg aagccgcgct tttcgagttc aggcagattg
     4141 atgttcatca ccggcggcgg tgcgtcgaac aggttcgcat ccttgaaccg gcgatagatc
     4201 tcgaagttca attcgctggt gatcgatttc atcgaggtga catcgctgac gtagcaataa
     4261 agctcgaagt tgaagacgcc gccggtaatc tcggcgaaga cgacaaaggg cgtcggtttc
     4321 ttcaggacgt tcttgttgct cttggtcacg tcgagaagaa cttcgcgtgc tttttgcggg
     4381 tcggcggcgg cgttcaccgg gatgttgagc ttgaggcggc ccgtcttgtc ggtacgcaca
     4441 aagtttttga cgacgccggc gacgagatcc gaattgggga tgacgactgc ggcgcggtcg
     4501 aacgtctcga tctcggttga acgtacattg atgcggcgca cgaagccttc gttggcgccg
     4561 acggaaatcc agtcgccgac acggatcgcc cgttcccaaa gcaggatcag gccggaaacg
     4621 aaattgttga cgatcgattg caggccaaag ccgataccaa cggacagcgc gccggcgacg
     4681 atggcgagtt tggcgaagtc gacaccgaga taggcgagcg caaagcccag cgcgaaaatg
     4741 cgcccgatat agccgatgct cgttttgatc gcgttgcgca gcccgaggtc gagccgtgta
     4801 tgtggcagaa agctcacttc gagccagcgc tcgactgcgc tcgtcgcggc ataaccggcg
     4861 gcgaaaatga ccagagtgaa gacgatgcgc gaaagcgaaa tcgtgacatc gccgatccgg
     4921 aaaccgaaga atgcggcgta gagatagcct gtcacatccg ccgattggac gccccacggc
     4981 accagtgccg cgagcacggc gataaggaaa atcaccacgc gagcgacgcc ggaagcaagg
     5041 atcgcgaatt gcgcgaacgc ctcgcggctc aatcctgtcg aaagcatcat ccggcggccg
     5101 aacggcgtcg tgccggtgat gccctggccg acaagctgat cgatcagcat gacgagcatg
     5161 accgccagac cgccgaggcc accgacccag atgatctggt cgattgcgaa acggccgagc
     5221 gacatgtagc cggcgaggac cgcggcaaag acgacaagga tcgcggccca gagcacggtg
     5281 cgcaggatgg aaaacaaatt cgccgtctgg tcgacgcgcg ggccgaaaac ctgctcgctt
     5341 tcctcctcat cgccgctctg gccgatccag agacttgccg caatcgcgat cgccgccagc
     5401 aggctgaaga acccgcgcgc gaacatatcg ctcgaccacg acgcctcgat cgcggagccg
     5461 accgcgttga agatgcggcc gcaggaaacc agcaccgtca cgatcaaaac ggtgcgcaga
     5521 acctgcgatg cggcatcgga atcgaccgcg atcagccgcc attgctcgcg cttgggcgcg
     5581 agcagcgcct gcgccagtcc ggcgccgatg gcgatgcgca cgacagaaaa gaagagcggg
     5641 cgcagcaacg gattctggtc gtcgacgagc ccgaagctgc gcgcgaggaa gccgatgaga
     5701 agcgcggtgg cgatcgggat ggcggcgatg acaaacagaa cgcgccacgc cgcgacgatt
     5761 ttctgcaacg gcgtcggatt gtccgccacg tctttgcgga agaacatgcg ctgcgcgaat
     5821 tgcgtcaccg gccaatacaa aatcccaatg agaacgagca gcccgcagaa tagggatgcg
     5881 cgccatccgg tcagtttggc agcgctgtcg ctgagccagc cgccagtccg gtgacgaagc
     5941 gcgcggagat ttctaggaac ttcccggacc acgtcggccc aaagcgacgg gctcgcgatg
     6001 cttggctcct gctgcaaaag cgcttccgtc agccgggcgc gcagccggga agcaatggtc
     6061 gtctgcgcct ggtcgacctg gacaatcagc agattggtgc gtttgaccaa tgcatcgacg
     6121 tcatcgaagg ccttttgccg cttcacccgc tcggccgcga cctctttgct ttccggcggt
     6181 gccttggcgt cgggcggcgc gccgagttga tcgagctggg ctttctgtgc ggcgagatgc
     6241 ggggtcaaat ggtcgagcac acgctgcagt gtcgggccga gtggcgccgt ctgctcctgc
     6301 agtttttgca aaacgtcttt ggaaagaccc ggctgctgca aagccgtctc gacctgctgc
     6361 aaggtattgg cgctggcgtc gaggttggcg gaattgatcg cgatgaacgt ctgcgcctgt
     6421 gccgcgatgc ccatgcagag caaaagtgcg gcgacagcaa agcttcggac gatagcgcgc
     6481 agaaaattca atggccccag ctcccttcga ttgcgcggcc ccaatatccg cctgtgcttt
     6541 ccgttcgccg ggaaagacgg cggcttcatg tcgctggcgg atagagatgc gtcaggccgc
     6601 gaaaatctcg cagatgatag cgcccgtctt catctggagc ggtgccgtcg agcgtcactt
     6661 tgccgaagcc accgggaata tcgagcatgt agcgcggctg acaaaggccg gagagtttcc
     6721 cgcgcaattc ttcgacgagc ttttggcctt cggcgaccga aaggcgaaaa tgcgaggtgc
     6781 cgcgcgcgag atccagatgg tgaagataat agggtttgat acgcgcggcg acgaaggcgc
     6841 gcatcagcgc cgcgagcgtt tcgacatcat cgttcacgcc gcgcagcaag acgctttggc
     6901 tgagcattgg aatcccggcg tctataagtt tggcgcaagc cgtttgcgcc tgcggcgtca
     6961 gttcccgcgc gtggttggcg tgaagcgcga catagatcgt cttgccggag cgccgcagta
     7021 gatcgacaag cgcatcgcta atcagttccg gcccgacaac cggcacgcgc gtatgaatgc
     7081 gaacaacctg cacatgcgtg gtggcgcgca agcgggcgaa tatttcttcg agacgacgca
     7141 gcgaaagaat tagcggatcg ccaccggtga ggatcacctc gaagatttcg tcatgcgccg
     7201 cgatataggc gaacgccttg tcgagcgcct cggacgacag atgggtcgcg ccgccggggc
     7261 cgacgctctc gcggcggaag cagaagcggc aatagaccgg gcagacatgc agaagcttaa
     7321 gcagcacgcg gtcgggatag cgatgcacga cgccctcgat ggggctcatc ggcgtgtcgc
     7381 caatcgggtc ggccaattcc tccggcgtcg tgatgagttc cgccgcgtcg ggaatgaatt
     7441 gtcgcgcaat gggatcagcg ggatcattgc ggtcgatcag cgccgccatc gtctcgttga
     7501 tcgcgatcgc ataaagatcg ccgaccctca tgagactctc gcgcgctgca ggtggtgcaa
     7561 acccttgggc aatcaggtcg tcaacgctgg tgagtgtcgc gtggctcatc gcgccgtccc
     7621 tgaatcggca attggcgtcc ataaaacatc ttcaatgcgc ctcgcgcccg tggcgagcat
     7681 cacaagccgg tcaaagccca gcgcgatgcc actcgcgggc ggcatttgcg caatcgccgc
     7741 gagaaaatcc tcgtcgatcg gataggcatc gctatagata cgttcacgcg cgatcatatc
     7801 gccgatgaag cgcgcgcgct gttcgttggc atccgtcaat tcgccgaagc cattggcgag
     7861 ttcgacgccg cagacataca gttcgaatcg ttcggcaaag cgtgtgtcgt gcttgagccg
     7921 cgccagcgcg gcttccggcg ccggataatc gacgagcaat gtcgcgcgac catgcccgag
     7981 gttcggttcg atcttggcgg aaagaatttt gctgaacaga tcagaccatg tgtcgtcgac
     8041 cgcaacgcgc aggccgagcg ccgctgtcct gtccgcgagc gccttgcggt cgcagctttg
     8101 cgcatcatag gtctggagga gatcgacgcc cgcatagcgc tgaaaagcgt ccgcgacggt
     8161 caaaatctcc ggttcggcga aagcgtccgc ggtcgcgtcg cgaaagcgaa atgtatccgt
     8221 ctgcgccgcc gttgctgcaa cggcgagcaa ctcgcggcaa tcggcgaaca acgccgtgta
     8281 gggcgcattc gtccgatacc attccagcat ggtgaattcg ggagggtgca gcggcccgcg
     8341 ctcgcggtta cggaagacgt ggccgaggct gaaaatcttc tcctcgccgg cggccagcag
     8401 ctttttcagc gcgaattccg gcgaagagtg caggtagagc cgcgatcttg catcatccgg
     8461 ggcgatcatt tcggtcgcga aagcggaaat atgcatctcg ttgcccggcg atatctggag
     8521 aatggcagtg tcggcctcga tgaagccgcg ggcgtcgaaa aacccgcggg cggccttggc
     8581 cagcgccgcc cgtgcggtca aaaagcgccg cttttcggcg tgcaccgcag ggtcccacca
     8641 gaaagaaggc ttgctcattt cctcatttcg cttgaaaacc cctcgccaag ccgggtagcg
     8701 atggtgcggg cgcttatagc gcagccgtgc gccgcgcggc agtccggccg gaattagcca
     8761 cctccggctg atccatattg aattttaatg cgttttgctc caagcggcgg ctcggatcaa
     8821 agcgccattc gagttaaacg aaggaaacct cgtgaaagtc atcgccagtt ccatccgcaa
     8881 gggcaatatt ctcgagcggg acgacggcca gctttatgtc gtgctgaccg ccgaaagctt
     8941 ccatcccggc aagggcacgc cgacgacgca gatcgacatg cgccggctgt ccgatggcgt
     9001 gaagacgacg gatcgctaca agacgacgga acaggtcgaa cgcgccttcg tcgaggatca
     9061 cgactacagc tacctttttc aggacggcga gcattatacg ttcatgaatg gcgagaccta
     9121 tgatcaggtc aacgtctcgg ccgatgtgat cggcgagcag gcggt
//
LOCUS       DASWOG010000058         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_202866,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000058 DASWOG010000000
VERSION     DASWOG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202866"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGB93_01155"
     CDS             <1..641
                     /locus_tag="VGB93_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0144768.1"
                     /translation="GEALFVDMGSTTTDIIPLKESQVAAQGYSDATRMAYGELVYAGI
                     VRSFLMAGLRLVPFGGRWVPIMNEAFTTTSDVYRILGDLPEDADLMPTADGQPKTIEA
                     SRARLARHIGLDAREVSAQALDQLAAFFGEAQLREIFDGASLVLSDALLNPAAPVVGA
                     GAGRFVVERLAARLGRAYIDIANLLPLAPALRGKAADCLPACAVALLAYAAA"
     gene            complement(626..1189)
                     /locus_tag="VGB93_01160"
     CDS             complement(626..1189)
                     /locus_tag="VGB93_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HEY0144769.1"
                     /translation="MPMIRETVVTTADAQGNVHVAPLGLIADGDHWIIAPFQPSTTLN
                     NLRAVPFAIANYIDDVRIFAGCLTGRPDWPLVATEDFIVPRLLAALTHAELSVVHIEE
                     HEQRPRFHCRVVKQVQHAPFEGMNRAKAAVLELAVLSSRLDFLPREKIEAEMAYLQIA
                     IDKTAGPEEAEAWSWLVEKVQRHYAAA"
     gene            complement(1190..>1919)
                     /locus_tag="VGB93_01165"
     CDS             complement(1190..>1919)
                     /locus_tag="VGB93_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0144770.1"
                     /translation="VKLAEKARNEDIPFIVDPILDPIHFGFGASLARYIEARRLMPDV
                     EMMMGTGNLTELTEADTSGITAMLLGLCSELHIRNVLVVQVSPHTRRTVAEHDAARRL
                     MFAARSDDALPKGYGAGLAQVHDLKPFPTSPPEIAERAADVRDTNYRIEVADDGIHLY
                     NRDGHHVARDAMALFPHLAVKGDDAHAFYLGTELMKAELAFDLGKRYVQDEPLDFGVA
                     TERAEDDLTRLRDMGHTLRAKAET"
BASE COUNT          348 a          585 c          596 g          390 t
ORIGIN      
        1 ttggcgaggc gctctttgtc gatatgggtt cgacgacgac cgacatcatt ccgctgaaag
       61 aaagtcaggt tgcggcgcaa ggctatagtg acgcgacacg catggcatat ggcgaattgg
      121 tctatgcggg tattgtgcgc agcttcctga tggccggcct gcggctcgtg ccgttcggcg
      181 gacgctgggt gccgatcatg aacgaggcat tcaccacgac gagcgatgtc tatcgcattc
      241 tcggcgatct cccggaggat gccgatctca tgccgacggc cgacgggcag ccgaaaacga
      301 tcgaagcctc acgggcgcgt ctcgcgcggc acatcggcct tgacgcacgc gaagttagcg
      361 cacaggcgct cgatcaactc gcggctttct tcggcgaggc gcaattgcgc gaaattttcg
      421 atggcgcgtc tctcgtttta tcggacgcgt tgcttaatcc cgccgctcct gtcgtcggcg
      481 caggggcggg ccgcttcgtt gtcgaacggc tcgcggcgcg tctcgggcgc gcttacattg
      541 atatcgctaa tttgcttccc ctcgcgcccg cattgcgcgg caaggccgcc gattgcctgc
      601 cggcctgtgc ggtcgctctt ctggcctacg cagccgcgta atgtctctgc actttctcca
      661 cgagccagct ccacgcctcg gcttcttcag gccccgccgt cttgtcgata gcgatttgca
      721 gataggccat ctccgcctcg attttctcgc gcggcaaaaa atcgagacgg cttgagagca
      781 ccgcgagctc gagcacggcg gccttggcgc ggttcatgcc ctcgaaaggc gcgtgctgaa
      841 cctgctttac gacgcggcag tgaaagcgcg gccgctgctc gtgctcctcg atatgcacga
      901 ccgaaagttc ggcatgtgtc agcgccgcga ggaggcgagg cacaataaaa tcctccgtcg
      961 caaccaacgg ccaatcgggc cgcccggtca ggcacccggc gaagatgcgg acgtcgtcga
     1021 tataattggc aattgcgaac ggaacggcgc gcaaattgtt cagcgtggtc gaaggctgga
     1081 acggtgcgat gatccaatga tcgccgtcgg caatcagacc gagtggcgcg acatggacgt
     1141 tgccctgcgc atcggccgtc gtcaccacag tttcgcggat catcggcatt caggtttctg
     1201 cctttgcgcg taatgtatgg cccatgtcgc gcagtcgcgt caggtcatct tccgcgcgtt
     1261 ccgtcgcaac gccgaagtcg agcggctcgt cctgcacata acgtttgccg agatcgaagg
     1321 cgagttcggc cttcatcaat tcggtgccga gatagaaggc gtgcgcgtca tcgcctttga
     1381 cagcgagatg cggaaacagc gccatcgcat cgcgcgcgac atgatggcca tcgcggttgt
     1441 aaagatggat gccgtcgtca gcaacttcga tccgataatt cgtgtcgcgt acatccgccg
     1501 cgcgttcggc gatctcaggc ggtgaggtcg gaaatggctt caagtcatgc acctgcgcga
     1561 gcccggcgcc atagcccttg ggcaaagcgt catcgctgcg cgcggcgaac atcagccgcc
     1621 gcgccgcgtc gtgctcggca accgtgcgcc gggtatgcgg gctcacctgc accacgagca
     1681 cattgcgaat atgaagttcg gagcagaggc caagcaacat ggcagtgatg ccgcttgtat
     1741 ccgcctcggt taattccgtc agattgccgg tgcccatcat catttcgacg tcgggcatca
     1801 gccgccgcgc ctcgatatag cgggcgagcg aggcgccgaa gccgaagtgg atcggatcaa
     1861 ggattggatc gacgatgaag gggatgtctt cgttgcgggc tttttccgcc agtttcacg
//
LOCUS       DASWOG010000059         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_294690,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000059 DASWOG010000000
VERSION     DASWOG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294690"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..670
                     /locus_tag="VGB93_01170"
     CDS             392..670
                     /locus_tag="VGB93_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144771.1"
                     /translation="MQTIVRCLIAAAFLGGAMPASAHFHHRPPAPRCWPAGPGLVVKG
                     SNYQQWDGSYASLADLTRSVNGTPCGVDCPAPSRVFFASAPPLYCEPY"
     gene            complement(675..1241)
                     /locus_tag="VGB93_01175"
     CDS             complement(675..1241)
                     /locus_tag="VGB93_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY0144772.1"
                     /translation="MDDGLISTIAQAAAPQSVELQECEAQDPAAQRIYAVRLHPHRSL
                     SERNFWILLCVFSAISFATTIPFVVMGAWPVAGFIGLDVAIFYFAFRANFRAAKAYED
                     VMVTPLDLSLEKVSAKGARAEWHFNPSWVSLHKETHDEYGVQKVALVSRGRSIEVGNF
                     LGPDEKAQVANDLSLALAEARRGPRYSD"
     gene            1240..>1709
                     /locus_tag="VGB93_01180"
     CDS             1240..>1709
                     /locus_tag="VGB93_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0144773.1"
                     /translation="MRALLLASAELYKPQMAQITRTKVTNTKGAGAKASKIKPKPAAK
                     VPAARASTPRGTRWPKPVPPETVIEIFQRFAVANPHPRGELLYKNPFTLLIAVVLSAQ
                     ATDAGVNKATPALFALADTPEKMLALGEDRVRDLVKTIGLYRAKAKNILGLSRAL"
BASE COUNT          366 a          535 c          491 g          317 t
ORIGIN      
        1 taactttagc catgtcgttt gtcctttcga gccgcaagac cggttggatc ccgaagcgat
       61 ccagcaatgg gcggacgggt aaaggccgcc cggtccccga tagaagtcaa tgtggtagcg
      121 ccgcaggagg gcgcaagatg aagtcgtata taagcgggtc atttttcgac gcaaggcagc
      181 gcttgggcct aagtgccaga ccacacaggc aaaatggggc tttgctgacg cgcacaacga
      241 ccgaattcgg cgtaaccgaa accgcggtta cggaagcggc cttcgcgggc attggaaatg
      301 gccaagtgcc ggcttcacat aaatcgctta tataccgacc taagctattc ctgccgcgtt
      361 gctgtgggca ttggttcgcc gggaggtttt gatgcaaacg attgtgcgtt gtcttatcgc
      421 tgcagccttt ctgggcggcg ccatgccggc ctccgcccat tttcaccatc gtccgccagc
      481 gccgcgctgt tggcccgcag gtcctggcct agtggtcaaa ggcagcaatt atcagcaatg
      541 ggacggcagt tacgcgtcgc tcgccgatct cacgcgttca gtcaacggaa cgccctgcgg
      601 ggtcgattgc ccggcgccat cacgggtctt tttcgccagc gcgccgccgc tctattgcga
      661 accctactaa gagatcagtc cgaatagcgc ggaccgcggc gagcctcggc cagcgccagc
      721 gacagatcat tggcgacttg cgccttctca tccgggccga gaaaattgcc gacctcgatg
      781 ctgcgtccgc gcgacacaag cgcgactttc tgcacgccat attcgtcgtg ggtttcctta
      841 tgcagcgaga cccacgacgg attgaagtgc cattcggccc gtgcgccctt ggctgaaacc
      901 ttttccagcg acagatcgag cggcgtgacc atcacatctt cgtaagcctt cgcggcgcgg
      961 aaattggccc ggaacgcgaa atagaagatc gccacatcga gaccaatgaa gccggcaacc
     1021 ggccacgcgc ccatgacgac gaacggaatc gtcgtcgcga aactgatcgc cgagaaaaca
     1081 caaagcagga tccagaaatt ccgctcactc agggagcgat gcggatgcag acggacagcg
     1141 tagatccgct gcgctgcggg gtcttgcgcc tcgcattctt gcaattcgac gctttgcggc
     1201 gccgctgcct gtgcgatggt ggagatcaga ccgtcatcca tgcgggctct tctcctagct
     1261 tccgccgaac tttataaacc gcaaatggca cagatcacca gaacgaaagt caccaacacg
     1321 aaaggtgccg gagccaaagc ctccaaaatc aagccgaaac cggcagcgaa agttccggcg
     1381 gcgcgcgcga gcacgccacg cggcacccgc tggccgaagc cggtgccacc cgagacggtg
     1441 atcgaaatct tccagcgatt cgctgtcgca aatccgcatc cgcgtggcga attgctctac
     1501 aaaaatccgt tcactttgct gatcgccgtc gtgctttcgg cacaggcgac cgacgcgggc
     1561 gtcaacaagg cgacgcccgc cctcttcgcc cttgcggaca cgccggagaa aatgctggcg
     1621 ctgggcgaag acagggtccg cgatctcgtc aagacgatcg gcctctatcg cgccaaagcc
     1681 aagaatatcc ttggactctc gcgcgccct
//
LOCUS       DASWOG010000060         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_258003,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000060 DASWOG010000000
VERSION     DASWOG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258003"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..256
                     /locus_tag="VGB93_01185"
     CDS             92..256
                     /locus_tag="VGB93_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0144774.1"
                     /translation="MNKIFARFMKDESGATAIEYGLIAGLIAVVIIGALTTLGSNIGK
                     KFNAVALNLS"
     gene            483..986
                     /locus_tag="VGB93_01190"
     CDS             483..986
                     /locus_tag="VGB93_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0144775.1"
                     /translation="MIFATLIFFPLLMAYAAFSDLLTMTISNFVSIALIVLFFSLALS
                     LGMPVREIGLHVAAGALMLAVTFIMFTRGWIGGGDAKLAAATALWFGFDHLFDYSLYA
                     AAIGGFLTLAILILRRHPLPRLLVRQAWLARLHEKGGGVPYGIALAIAGLLLYPETSI
                     WAAAITR"
     gene            1122..1916
                     /gene="cpaB"
                     /locus_tag="VGB93_01195"
     CDS             1122..1916
                     /gene="cpaB"
                     /locus_tag="VGB93_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0144776.1"
                     /translation="MKTARLIILGSAALAGVGAFIMILGSGKQPAPVRIAIPAPPPPM
                     DQVLVANRDIPFGNMVDRGDLRWQSWPPNAAPAGAILETRDPKGADEFNGWLASVHIS
                     EGEPVYRNHLVQLGKEGVLATLLPAGMRAVAITIDTGGSSTAGNFIQPGDHVDVIHTF
                     RDDDVARAAGGNGMVSETILHNIRVLAIGEAIQKPGREPVISGPNATLELTPKEVEQI
                     ILAQKTGQLSLSLRSMVDSNASADRDAPDKDVNLTIVRFGIAVPGR"
     gene            1961..3400
                     /locus_tag="VGB93_01200"
     CDS             1961..3400
                     /locus_tag="VGB93_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY0144777.1"
                     /translation="MKRRVSTFVCGLSASLILLAPAQDILAQSRLQVEDGNSTALTRS
                     ISMGVGKSVIVDLPEDASEIFVGDPKVANAIVRSARKLYVIGLDNGQTTVFAMDKAGH
                     QIAALQISIGRDIGELQRILRVAMPTSNIVTQTINDTIVLTGEVDSPEEAQRAEDIAQ
                     GFVKDVNSSGGAPAAGLVVNSLTIRGRDQVMLKVTVAEVQRNIAKQLGLTSSTWGPFT
                     QFNPFGINGNIATSPTGQSSGITLHNPSNTVSATLQAFERYGVTHVLAEPTVSAVSGQ
                     TATFVVGGEFPIPGPPTTCPGTTSTSAVCSGGAIYQNYGISLVFAPIVLSEGRILLHL
                     STEVTDIDNTKTVFIQGVPTPGLLTRKNSTSVELPSGGSIVSAGLLQTESAQVINGFP
                     GLMNLPILGTLFRSRDYQKQETELMIIVTPYLVQATSAASLSRPDDGFADSSDPQAWL
                     LGRVNRLYASPNNPEAIKNFKGRVGFIVN"
     gene            3426..4169
                     /locus_tag="VGB93_01205"
     CDS             3426..4169
                     /locus_tag="VGB93_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEY0144778.1"
                     /translation="MPTLPKKVGSRWTGSAFILPLSALTLLGGCAGNVDRMRTSSIAM
                     DDYRNRHPIVLAEAAQKLDIFPPPELRGLDRRTYAQVAEYGRRYHYNGQGAIQIFVPT
                     GANGGVSPSTIQSIRRALAAGGAYAPLQVTTYPVVNGGLASPLRLSFYGLKAKVADPC
                     GQWPNDLASGGGLQGWDNKPYWNYGCSYQNMMAEQVADPRDLVGPRAEDPADTEMRAR
                     AIDSIRKGTDPSTDWKVKNTAISNVGVDQ"
BASE COUNT          841 a         1313 c         1176 g          905 t
ORIGIN      
        1 taagcggttt tgagcaaaag ctccttattg tcgcttttgt tccgccaccg agccggaatg
       61 attttttgaa ttcgcgaacg aggaaactac catgaataag attttcgccc gctttatgaa
      121 ggatgagtcc ggtgcgacgg ccatcgaata cggcctcatc gctggcttga tcgctgtcgt
      181 gatcatcggc gccctgacca cgttgggcag caacatcggc aagaagttca acgccgtcgc
      241 cctcaatctg agctaagttc aactgtcgcg ctttatctac ggcccgcgct actcacatgg
      301 cgcgggccgt agatgttttg gaccacagtt tcgttttggg acaattgccc ccgctgccag
      361 cggggcgacg ccgtttcgcg gcgcccacac cgtgccggcc taagccgtta agacttggtg
      421 aacgcgccat cgctacgctg cacgacaccg cacatctcct attctaaggc agagcttgga
      481 tcatgatctt cgcgacgctc attttttttc ccctgctcat ggcctatgcg gctttttccg
      541 atctgctgac gatgacgatt tccaatttcg tctcgatcgc tctcatcgtg ctctttttta
      601 gtctggcttt gtcgctgggt atgccggtca gagaaattgg cctccatgtc gccgccggtg
      661 cactgatgct cgccgtgacc ttcattatgt tcacgcgcgg ctggatcggc ggcggtgacg
      721 ccaaactggc cgcagccacg gcgctgtggt tcggcttcga ccacttgttt gactacagcc
      781 tttatgccgc ggccatcggc ggcttcctga ccttggcgat cttaattctg cggcggcatc
      841 cgttgccgcg actcctcgtc cggcaggcct ggctcgcccg gttgcacgag aagggaggtg
      901 gtgtacccta tggcatcgcg ttggcgatcg ccgggctcct gctctatccc gagacgagta
      961 tctgggccgc agctatcacc cgttagccag tctgccccga gccaaactgt cttaggtcgg
     1021 ggcaactcgc cgcaatcgga gagatttggt taaccataat ttgacgtttg cgtggtcgaa
     1081 tccaggacga gtttaagcaa ccgcgtcgaa cgggttccac catgaagacc gcccgcctca
     1141 tcatcctcgg ctccgccgcg ctcgccggcg tgggggcgtt catcatgatt cttggcagcg
     1201 gtaaacaacc cgcgcccgtt agaattgcca ttccggcccc accgcccccc atggatcagg
     1261 tgttggttgc caatcgcgac attccttttg gcaacatggt cgatcgcggg gatttacgct
     1321 ggcaatcctg gccgccgaat gctgcgcctg caggcgcgat tctcgagact cgggatccaa
     1381 aaggcgcgga cgaatttaac ggttggctcg caagcgtcca tatcagcgag ggcgaacccg
     1441 tctatcgcaa ccacctcgtt cagctcggta aggaaggcgt cttggcgacc cttttgccgg
     1501 ctggtatgcg cgctgtggcg attacgatcg acacgggggg cagcagcacg gcgggcaact
     1561 ttatccagcc cggcgaccat gtggatgtga tccatacttt cagggatgac gacgtcgctc
     1621 gcgccgccgg cggcaacggc atggtgagcg agacgatcct ccacaacata cgcgtgctcg
     1681 ccatcggcga ggccattcaa aagcccggca gagagccggt catcagtggc ccgaatgcaa
     1741 ccttggaact tacgcccaag gaggtcgagc aaatcattct tgcccagaaa accgggcaac
     1801 tttctctgag tttgcggagc atggtcgatt caaatgccag cgccgatcgg gacgcgcccg
     1861 ataaggacgt aaatcttacc atcgttcgat tcggtatcgc tgttcccggc cgttgagttg
     1921 aatgcggaca aaaagcacta ggctcttaag gaatttctgc atgaaacgcc gcgtctcgac
     1981 ttttgtctgc ggattgtccg catcgctgat tctgcttgcc ccggcccagg atattctcgc
     2041 gcaatcccgg ctgcaggttg aagacggcaa cagcaccgcc ctcacgcgca gcatctcgat
     2101 gggtgtcggc aaatcagtga ttgtcgatct tccggaagac gcgtccgaaa ttttcgttgg
     2161 tgatccgaag gttgcgaatg caatcgtgcg ctcggcccgc aaactctatg tcatcggtct
     2221 cgataatggc cagacgaccg ttttcgcgat ggataaagcc ggccaccaga tcgccgcact
     2281 gcaaatcagc atcggccgcg acattggtga attgcagcgg attcttagag tggcgatgcc
     2341 cacctcgaat attgttaccc aaaccattaa cgacacaatc gttctcaccg gcgaggtcga
     2401 ttcgcccgaa gaagcgcagc gcgccgaaga catcgcgcaa ggtttcgtca aggacgttaa
     2461 ctcttcgggc ggagcaccgg ccgccggtct tgtcgtgaat tccctcacca tacgcggccg
     2521 cgatcaggtg atgctgaaag tcacggtcgc cgaagtccaa cgcaacatcg ccaaacagct
     2581 cggcctcaca tcgagcactt gggggccttt cacgcaattt aatcccttcg gcatcaacgg
     2641 caacatcgca acgtcgccga ccggacagtc gagcggcatc acgctccaca atccgtcgaa
     2701 cacggtgtca gcgacgttac aggcattcga gcgttatggc gtgactcatg tcctggcgga
     2761 gccgaccgtt tccgctgttt cgggtcagac cgcaaccttc gtcgtcggcg gcgaatttcc
     2821 aatccccggt ccgccgacga cttgcccagg cacaacatcg acgtcggctg tctgctccgg
     2881 cggtgcgatc tatcagaatt acggcatcag tctggtattc gcgccgattg ttctcagcga
     2941 gggtcgcata cttttgcatc tctcgaccga ggtgacggat atcgataaca ccaagactgt
     3001 ctttatccag ggtgtgccga cgccgggcct gctgacccgc aagaattcga ccagcgtcga
     3061 attgccgtcc ggcggctcga tcgtctcggc cggcttgctc caaacagagt cggcgcaggt
     3121 catcaacggc tttcccggcc tgatgaacct gccgatactc ggcacgctct tccgctcccg
     3181 cgattatcaa aagcaagaga ccgagctgat gatcatcgtc acgccttatc tggtgcaagc
     3241 gacgagcgct gcatccttgt cgcgcccaga cgatggtttc gcagattcaa gcgatccgca
     3301 ggcttggctg ctcggccgcg tcaatcgcct ctacgcctca cccaacaatc ccgaggcgat
     3361 caagaatttc aaaggccgag tcggctttat cgtgaactga actgcctggg gaaatggacc
     3421 gaaccatgcc gaccttgccg aaaaaagttg ggagccgctg gacaggctca gccttcatcc
     3481 tcccgctgag cgccttgacg ctgctgggtg gttgcgcggg caatgtggat cgcatgcgaa
     3541 cctcgtccat cgccatggac gactatcgca accgccatcc gatcgtgctt gccgaggccg
     3601 cccaaaagct tgacatcttc ccgccgccgg agctgcgtgg actcgatcgc cgcacatacg
     3661 cgcaagtcgc tgaatatggt cgccgttatc attataacgg ccaaggcgcg atccagattt
     3721 tcgttccgac aggggcaaac ggcggggtaa gcccatcgac gatccaatcg atccgccggg
     3781 ccctcgcggc cgggggcgcc tacgcccctc ttcaggtcac aacttatccg gtcgttaatg
     3841 gcggcctcgc atcgcctttg cgcctcagct tctatgggct caaggcgaaa gtcgccgatc
     3901 cgtgtggcca atggccgaac gacctcgcgt ccggcggtgg cctgcaaggc tgggataaca
     3961 aaccttactg gaactacggc tgctcctatc agaatatgat ggccgaacag gtagccgatc
     4021 cgcgcgatct cgtcggtccg cgcgccgaag atccggctga caccgaaatg cgcgcccgcg
     4081 ccatcgatag catccgcaaa ggcactgatc cgtccacgga ttggaaagtc aagaacaccg
     4141 ccatcagcaa cgttggggtc gaccaatgac cgacggacac gacccttcgc ttgagcaaat
     4201 cgcgcccgtc ccgcgcattt cgatgcaggc gtttt
//
LOCUS       DASWOG010000061         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_74666,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000061 DASWOG010000000
VERSION     DASWOG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74666"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGB93_01210"
     CDS             complement(<1..435)
                     /locus_tag="VGB93_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEY0144779.1"
                     /translation="MMRSHQKFAVSSHAAAARADEAELGALPEWSLGDLYPAMDSPQF
                     VADLSRAEDECKAFAEEYRGKLASLAAQDFADPQSHLLTQAVRRYEALDDLTGKIGSY
                     ASLVYSGDTSDQVRVKFYGDTQEKLNAAWTELLFFQLELNRLD"
     gene            714..968
                     /locus_tag="VGB93_01215"
     CDS             714..968
                     /locus_tag="VGB93_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144780.1"
                     /translation="MNVKATIAASSLALAFFASTGANAAMHHHHHRMSVEMMGHTTAT
                     LNDGTKLDVDIVKMNGHMMVAIPADELPDYLHQQIFHVQN"
     gene            1252..>2693
                     /locus_tag="VGB93_01220"
     CDS             1252..>2693
                     /locus_tag="VGB93_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0144781.1"
                     /translation="MSSLVLIVDDDPVQRRVLETLVRRFGYECETVDSGAAALQRLQS
                     RSPEIDLIILDLVMPDVDGMTVLARMREQKSPVPAIVQTAHGSIDAVISAMRAGAIDF
                     VVKPVGAERLQVSIKNALRVEALEDEVRRLNRHASGTLTFKDLRSKSEDMDRVVRLGE
                     RAAKSTIPVLLEGESGVGKEVIARAIQGGSDRRTKPFVTVNCGALPENLVESILFGHE
                     KGAFTGATDKHLGKFTEANGGTLFLDEVGDLPPDAQVKLLRALQDGEIDPVGAKRPIR
                     VDVRIISATNQDLIDLVKRGIFREDLYYRLNVFPITVPALRVRRDDIADLARRFMARF
                     AAEEGKPLRGIGAEALALLSSYLWPGNVRQLENAMFRAVVLADGDELTVAEFPQIAAQ
                     VAGFDVRIPPAPASLVRPTRPEREVVRVEVRDPNVLPLLDGAGNLRQLDHLEAETIKF
                     ALAYYRGQMSAVARKLGIGRSNLYRKMKEYG"
BASE COUNT          538 a          844 c          794 g          517 t
ORIGIN      
        1 gtcgagccga ttgagttcga gctggaagaa caaaagctcg gtccacgcgg cattcaattt
       61 ttcctgcgta tcgccgtaga atttcacccg cacctggtcg ctggtgtcgc cggaatagac
      121 gaggctcgcg tatgaaccga tcttgccggt gagatcgtca agcgcctcat agcgacgaac
      181 ggcttgcgtc agcagatgcg attgcggatc ggcaaaatct tgcgcggcga gcgacgcgag
      241 cttgccgcga tattcttccg cgaaggcttt gcattcgtct tccgcgcgcg aaaggtctgc
      301 cacgaattgc ggcgagtcca tcgccggata aagatcgcca agcgaccatt ccggcaaagc
      361 acccagttct gcctcatcgg cccgcgccgc ggccgcatgg gaggaaactg caaacttctg
      421 gtgtgagcgc atcatcgatc cgagccggcc tcatggtgaa aaagggcttt tagctcagcc
      481 gcgcgtgcgg tgaaagtgaa acgcagagcg gccgctgcct tacttcgcag cagcaaactg
      541 gcgcgttacg ccgccgcatg gaatttccgt ctcacttttt attgacgcgc cgtagcggca
      601 cggcccgtaa cgctgcgccg acgatttgcg aaacctcgcg tgtacggctg gattctctgg
      661 ccggccgcgg gagccgatcg cgcgcctcgt gcatcatcaa aaggaagaac actatgaacg
      721 tcaaagccac gatcgccgcc agttcgctcg ctctcgcttt tttcgcctcg acgggcgcca
      781 acgccgctat gcatcatcat caccaccgca tgtccgtcga aatgatgggc catacgaccg
      841 cgacgctcaa cgacggcacc aagctcgacg tcgatatcgt gaaaatgaac ggccacatga
      901 tggtcgcgat ccccgccgac gaactgccgg actatctgca ccagcaaatt ttccacgtac
      961 aaaactaaga cattcaataa ttttcatggg gcgcgtcgcg aagagcgccg cgccccattt
     1021 ttattcataa aattttaacg gctgcgctgg gggcgccgcc cgctggcgag tgtcccgcaa
     1081 taacgcgggc attaacatcg catttaccct gatgggcgag catggatttg gatggcggcg
     1141 cagaaccgac actcggcagc gcccccgcca taccgctggt tacacgagca accccgccag
     1201 gggccgcagg tccgttctgc tccgggctgc cgcctgtcga agaaagtgca aatgtcttca
     1261 ctcgtgctga ttgtcgacga cgatccagtg cagcgccgcg ttctcgaaac gcttgtccgc
     1321 cggtttggct atgagtgcga aacggtcgat agcggcgcag ccgcactgca gcgcctgcaa
     1381 tcgcgctccc ctgagatcga tctgattatt ctcgacctcg tgatgccgga tgtcgacggc
     1441 atgacggtac tggcacggat gcgcgagcaa aaaagccccg tccccgccat cgtccagacg
     1501 gcacacggtt cgatcgacgc ggtgatctcg gctatgcgcg ccggggcgat cgatttcgtc
     1561 gtcaagccgg tcggggccga gcggctgcaa gtgtccatca aaaacgctct gcgcgtcgag
     1621 gccttggaag acgaagtgcg ccggctcaac cggcatgcct cgggaacgct gaccttcaag
     1681 gatctgcgca gcaaaagcga ggacatggac cgtgtcgtgc gcctgggcga acgcgccgcg
     1741 aaatcgacga tcccggtcct gctcgaaggc gaatcaggcg tcggcaaaga agtcatcgcg
     1801 cgggcgattc agggcggctc cgaccgtcgc accaagccgt ttgtcactgt caattgcggc
     1861 gccctgccgg agaacctcgt cgaatcgatt ctcttcgggc atgagaaagg cgccttcacg
     1921 ggtgccaccg acaaacatct cggcaaattc accgaggcca atggcggcac gctcttcctc
     1981 gatgaagtcg gcgatctgcc gcccgacgca caggtgaagc ttctgcgcgc cttgcaagat
     2041 ggcgagatcg atcctgtcgg cgcgaagcgg ccgatccgcg tcgatgtccg aatcatctcg
     2101 gcaacgaatc aggacctcat tgatctcgtc aaacgcggca ttttccgcga ggacctttat
     2161 taccgcttga acgtctttcc catcaccgtt ccggcgctcc gcgtgcggcg ggatgatatc
     2221 gccgatcttg cgcggcgctt catggcgcgt tttgcggcgg aagaaggcaa accgctgcgt
     2281 ggcatcggtg cggaagcgct ggcgctgctc agcagttatc tttggcccgg caatgtgcgg
     2341 caattggaaa atgccatgtt ccgggcggtc gttctcgccg acggcgacga actgaccgtc
     2401 gcggaatttc cgcagatcgc ggcgcaggtc gccggcttcg acgttcgcat cccgccggca
     2461 ccggcctcgc ttgtacgtcc cacgcgaccg gaacgcgaag ttgtccgcgt cgaagtgcgc
     2521 gacccgaacg tcctgccctt gctcgatggg gcaggtaacc ttcggcaact cgatcatctc
     2581 gaggccgaaa ccatcaaatt cgcgctcgct tattaccgcg gccagatgtc cgccgtggcg
     2641 cgcaagctcg gcatcggccg ttcgaatctc tatcgcaaga tgaaggaata cgg
//
LOCUS       DASWOG010000062         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_313065,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000062 DASWOG010000000
VERSION     DASWOG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313065"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VGB93_01225"
     CDS             complement(<1..773)
                     /locus_tag="VGB93_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEY0144782.1"
                     /translation="MALILISAALLALSSLARAAVATPNLAVGPQYVTTHVYVAPRDI
                     DRFVESFVATFGGTATKKQLVNVTPTPSTTEWRAVSSPVGALSVFGFTTPYPWPFGAE
                     RNGFLVSDFDAAVKAARADGAGFLTSPFEDPIGRDAVIQWPGGVNMQIYWHTKAPHYP
                     PLAAIPENRVYVAPEQADAFIRAYLAFSQGKVIKDNRKAPGIEIGEPFNIFRRVLIDS
                     GFGKTLIFATDGQMPYPWGRETTGYEVRDLDATILKAEAA"
     gene            complement(1143..2669)
                     /locus_tag="VGB93_01230"
     CDS             complement(1143..2669)
                     /locus_tag="VGB93_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0144783.1"
                     /translation="MSESGGETKGPDLTRGVPLTQIGESGMLLGHVGDEAVLVARSGD
                     ELFAIGTTCTHYQGPLAEGLIVGDTVRCPLHHACFSLRTGEALRAPALDPVGAWRVEQ
                     RDGSVYVTDKIKPAPKPGPSGAELPKKIVIVGGGAAANAAAEMLRREGFAGAITMLSA
                     DDSVPVDRPNLSKDYLAGEAQESWIPLRSADFYKKHGIDLVLKARVTGLDAGAKEVRL
                     ADGTAHSYDSLLLATGATPVHLDIPGADQIYYLRSFDDSRAIIAAAEKAKRAVVIGAS
                     FIGLEVAASLRKRKLEVHVVGPDQHPLEKVVGPEVGDFVRGLHEAQGVQFHMGTTATA
                     IDERGVTLANGVTLPADLIVAGVGVRPAIELADQAGLKIDHGISVDQYLETSVPGIFA
                     AGDLARWPDPLTGQNIRVEHWVVAERQGQVAALNMLGKRQRYDDVPFFWSQHYDVSIR
                     YVGHAEKWDKVMIDGDLATKDCEVTYLRDGRKLAVVTIGRDRQSLIAEAGLEALIAES
                     "
     gene            complement(2690..3583)
                     /locus_tag="VGB93_01235"
     CDS             complement(2690..3583)
                     /locus_tag="VGB93_01235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144784.1"
                     /translation="MKETAMDVGFIGLGGMGSAMASRLIAAGHRVLVWNRSQAAAEKL
                     RAEGAEVVASPGDAFQADAVLTMLASDEVIEELIIAPGLLKKARPGLVHLVTATISVD
                     FAKTLEKLHAEAGVAYIGAPVLGRPDVAAKGELNVLAAGKPDDIARVKPLLDAIGKAT
                     WVVGTEPHLANVAKLAANFTLVSAIETMAEAFAFCERQNVAPEKIAEILTGTLFAAPA
                     YKTYSAAILERKFEAGFKLSLGLKDVRLMLAASEKAGAPLPFASVVRDNFVDAIAHGE
                     GDKDWSAVSRVAFRRANLAND"
BASE COUNT          657 a         1121 c         1166 g          702 t
ORIGIN      
        1 gcggcttccg ccttcaaaat cgtcgcgtcg aggtctctta cctcataacc cgtcgtctcg
       61 cgtccccacg gatagggcat ctgaccatcg gtcgcgaaga tgagcgtctt gccgaagccg
      121 gagtcgatca gcacgcggcg aaaaatattg aacggctcgc cgatctcgat gccgggcgcc
      181 ttacgattat ccttgattac cttgccttgc gaaaacgcaa gataggctcg gatgaaggcg
      241 tccgcttgtt ccggcgcgac ataaacacgg ttctccggga ttgcggcgag cggggggtaa
      301 tgcggcgcct tcgtatgcca atagatctgc atgttgacgc cgcccggcca ttggatcacc
      361 gcatcgcggc ctatcggatc ttcaaacggc gaggtaagga agccggcgcc atcggcgcgg
      421 gcggctttca ccgcggcatc aaaatcggag acgagaaagc cattgcgctc ggcaccgaag
      481 ggccagggat agggcgtcgt gaaaccgaag acggataggg cgccgacggg tgaggaaacc
      541 gctcgccatt ccgttgtgct tggcgtcggc gtgacgttga cgagctgttt cttggtggca
      601 gtgcccccga aagtcgcaac gaagctttcg acgaaacggt cgatgtcgcg cggggccaca
      661 tagacatgcg tcgtaacgta ttgcggcccg acggcgaggt tgggcgtagc cacagccgcg
      721 cgcgcaaggc tgctgagggc gagcagcgcg gcggagatca gaatcaaagc cattctcatc
      781 ttttgcatcg tccttcttcg cgccagtcct tttttgcgcc aagctgcgcg ggctttggct
      841 agcgaagatt tggcagcttg taaccacaaa aaatcttgcc ggtttcataa ggcttgggca
      901 tgatcgtcac gcgtctgtct gttgatcatg ctgtgcggcc cttgcgctga tgtcgcgaag
      961 gtcgccgttg atcacagtcg cgcagaggcg gcgcccgcag ggcgccccgc aataggagtt
     1021 aaggacgatg gcgaagggtc agatccgcgg caataaagaa gccaagaaac cgaagaagga
     1081 aaagcccaag acgatagctg cggcaccgtc ggtcaaggac gtcgtcggca agaaaaagta
     1141 gtttaggact cggcgatcag ggcttccagg ccagcttccg cgatcagcga ctgccgatcg
     1201 cggccaatgg tcactacggc aagtttgcgg ccgtcgcgaa ggtaggtcac ttcgcagtcc
     1261 ttggtcgcca gatcgccgtc aatcatcacc ttgtcccatt tctcggcgtg cccgacgtag
     1321 cgaattgaga cgtcgtagtg ctggctccag aagaagggca cgtcgtcgta gcgctgccgt
     1381 ttgccgagca tgttgagcgc cgcgacttgg ccctgccgct cggcgacgac ccaatgttcg
     1441 acgcggatgt tctggccggt cagcggatct ggccagcgcg ccagatcgcc ggcggcgaaa
     1501 atgcccggca cgctcgtttc gagatattga tcgacgctga tgccgtggtc gatctttagg
     1561 cccgcctgat cggcgagttc gatggcgggc cgcacgccga cgccggcgac gatcaaatcc
     1621 gctggcaggg tcacgccatt agcgagcgtg acgccgcgct cgtcgattgc cgtcgccgtc
     1681 gttcccatat ggaattgcac cccctgcgcc tcgtgcagac cgcggacgaa gtcgccgacc
     1741 tccggcccta cgactttttc gagcggatgc tgatccggcc ccacaacgtg gacctcaagc
     1801 ttgcgtttgc gcagcgaggc cgcgacttcg aggccgatga agcttgcgcc aatgacgacg
     1861 gcgcgtttgg ccttctcggc tgctgcaatg atggcgcggc tgtcgtcgaa gctgcgcaga
     1921 taatagattt gatcggcgcc cgggatatcg agatgcacgg gggtggcgcc agtcgcgagc
     1981 agcagactgt cgtaactatg cgctgtgcca tccgcgagcc gcacttcttt ggcgcccgcg
     2041 tcgaggccag tcacccgcgc tttcagcacc aagtctatgc cgtgtttctt gtaaaaatcg
     2101 gcggagcgca gcgggatcca ggactcttgc gcttcgccag cgaggtaatc ctttgagagg
     2161 ttgggcctgt cgaccggcac cgaatcgtcg gcgctgagca tggtgatcgc gccggcgaaa
     2221 ccctcccgcc gcaacatttc cgcagccgca ttggcggctg cgcccccgcc gacgatcacg
     2281 attttctttg gaagttcggc ccccgacggg ccgggcttgg gcgcaggctt gatcttgtcg
     2341 gttacgtaga cgctaccgtc gcgctgctcg acgcgccagg cgcctaccgg gtcgagcgcc
     2401 ggcgcccgca gagcttcacc ggtgcgcaga ctgaaacagg cgtggtgcaa ggggcagcgc
     2461 acggtgtcgc cgacgatcaa tccttcggcg agcgggcctt gataatgcgt gcaagtcgtg
     2521 ccgatcgcga aaagctcgtc gccactgcgg gcaacgagaa cggcctcgtc gccgacatgg
     2581 ccgagcagca tgccgctttc gccgatctga gtgagcggca cgccgcgtgt gagatcagga
     2641 cctttggtct cgccgccaga ttcgctcatc ccaattctcc tggtttgcgt cagtcgttgg
     2701 cgaggttggc gcggcggaag gccacgcggg aaaccgccga ccaatccttg tcgccctcgc
     2761 catgcgcgat cgcatcgacg aaattatccc gcacgacgct ggcaaagggc agcggcgcgc
     2821 cggctttttc gctcgccgcg agcatcagcc gcacatcctt cagtcccagc gacaatttga
     2881 atcccgcctc gaatttccgc tccaggatgg cggcactgta ggttttgtaa gccggggcgg
     2941 cgaacaaggt tccggtcagg atttcggcga tcttttccgg cgcgacgttc tggcgttcgc
     3001 agaacgcaaa ggcctcggcc atggtttcga ttgccgaaac aagggtgaaa ttggcggcga
     3061 gcttggcgac attggcgaga tgcggctcgg tgccgacgac ccaggttgcc ttgccgattg
     3121 cgtcgagcag gggtttgacg cgggcgatgt cgtccggctt gcccgcggcc agcacgttga
     3181 gttcgccctt cgccgcgacg tcagggcgtc cgagtaccgg cgcgccgata taggcgacgc
     3241 cagcctcggc gtggagcttt tcgagcgtct tggcgaagtc cacggagatc gtggcggtga
     3301 cgagatggac gagaccgggg cgcgctttct tcaggagacc gggcgcgatg atcagttcct
     3361 cgatcacctc atccgaggcg agcatggtca gcacggcatc ggcctgaaag gcatcgccgg
     3421 ggctggcgac cacctcggcg ccttcggcgc gcaatttctc ggccgccgcc tgcgaccggt
     3481 tccacaccag aacccgatgc ccggcggcga tcagccgcga tgccatggcc gagcccattc
     3541 cgcccagtcc gataaacccg acgtccatgg ctgtctcctt tatgctattc gctttgcggt
     3601 atgcggactt gccgtcacat tgtgccggcc gcgcccggtg cttatt
//
LOCUS       DASWOG010000063         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_147907,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000063 DASWOG010000000
VERSION     DASWOG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147907"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..>1566
                     /locus_tag="VGB93_01240"
     CDS             428..>1566
                     /locus_tag="VGB93_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0144785.1"
                     /translation="MVSVTDEQPMMERPRPKLPRRLGLSGKLLLLTIPLVMIAEILIY
                     VPAIANFRMNRLNDRLAAANTAALVLDAAPSGLVPESLSRQILHSIGARAVAIKMGQQ
                     RRLLASTDLPQGIAHDIDMRSLTVWSAIIDSFEIMMETGNQAIRVIGPTPGGGQFIEV
                     VIDEAPLRQAMYRFSSNLLVVSLIIAILTAALVYLALHFLFVRPMRRLTASLVAFHEN
                     PESSARIIVPSHRGDEIGVAERELSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNL
                     LASSQLLSDQLSSVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGRAQEASPDRRMMLI
                     EPVVAEVRESAGLATDASISWISAIERGLTIDADPDQLFRVLINLV"
BASE COUNT          288 a          458 c          485 g          335 t
ORIGIN      
        1 tttgttgccg ttcaaactga caacttgcct gatatagagg taagtccgcc gatgctggcg
       61 ggcccaaatc agggccgttt ccggcccaaa tcgagccgcc ccggtgaacc gggtcatcgc
      121 ggacagttgg cgcggcttat acgggagcga cctgttttcg tcaacgctgc cgcgccctct
      181 aatcagggtc ctttgaaacg gcgcaattgg gacgaataaa tcagaaatcg cggtgtttta
      241 agtgcgagga tgatgacacg ccatccgggt cggctttttg ccgatttggg gtcattttgg
      301 cgaattcgag caattctcgg gttcccgcgt gtcaatcccg gaaaatggca gctatccatt
      361 ataaactgtc atttgaccga gtgcagtgca caacgcattg tcgaaaggca tgattccaaa
      421 ggtagttatg gtgtcagtga ccgacgaaca gccaatgatg gaaaggccgc ggcccaagct
      481 gccgcggcgg cttggcttgt ccggcaagct attgctgctg accattccct tggtgatgat
      541 cgcggaaatc ctgatttacg tacccgcgat cgctaatttc cgcatgaacc ggctcaatga
      601 ccggctggcg gcggccaata cagctgcgtt ggtgctggat gccgcgccct ccggactggt
      661 gccggaatcg ctgtcgcggc agatcctcca cagcatcggc gcgcgggcgg tggcgatcaa
      721 gatgggtcag cagcgccggc ttctggccag taccgatttg ccgcaaggta tcgcgcacga
      781 catcgacatg cgttcgctga cggtgtggtc ggcgatcatc gattcctttg agatcatgat
      841 ggaaaccggc aatcaggcga tccgcgtgat cgggccgacg ccgggcggcg ggcagttcat
      901 cgaggtcgtg atcgacgagg cgccgctgcg ccaggcgatg taccggtttt ccagcaatct
      961 tctggtggtg tcgctgatca tcgcgatcct taccgcggcg ctggtgtatc tggcgctgca
     1021 tttcctgttc gtgcggccga tgcggcggct gaccgccagc ctagtcgctt ttcacgaaaa
     1081 tcccgaaagc tcagcccgga tcatcgtgcc gtcgcatcgc ggcgacgaga tcggcgtcgc
     1141 cgagcgcgaa ttgtccgaca tgcagcgcga cctggtgtcg atgctgcatc aaaagagccg
     1201 gcttgccgcg cttggtcttg cggtatcgaa gatcaaccat gatctgcgca acctgctggc
     1261 ctcctcgcaa ttgctgtcgg accaactctc cagcgtgccc gatccgcggg tgcagcgctt
     1321 tgcgccgaag ctgatgcgct ccctggagcg cgccatcgcg ttctgccagt cgacgctctc
     1381 ttatggccgc gcgcaggaag cttcacccga tcgccggatg atgctgatcg agcctgtggt
     1441 ggccgaagtg cgcgaatcgg caggcctcgc gacggatgcg tcgatcagct ggatcagcgc
     1501 gatcgagcgc gggctgacca tcgacgccga cccggaccag ttgtttcgag tgctgatcaa
     1561 tctggt
//
LOCUS       DASWOG010000064         6369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_349630,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000064 DASWOG010000000
VERSION     DASWOG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349630"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1484
                     /gene="rimO"
                     /locus_tag="VGB93_01245"
     CDS             138..1484
                     /gene="rimO"
                     /locus_tag="VGB93_01245"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0144786.1"
                     /translation="MTREPSAATTAASPPKISFVSLGCPKALVDSERIITRLRAEGYE
                     LTRSHAGADAVIVNTCGFLDSAKAESLAAIGAALEENGKVIVTGCMGAESETIRSAYP
                     NVLAISGPQAYESVVGAVHDAIAPPHDPFLDLVPAQGIKLTPRHYAYLKISEGCNNRC
                     SFCIIPRLRGDLVSRPAADVLREAEKLVAAGVKELLVISQDTGAYGLDLRYAESTLGD
                     RQVRAKFIDLARELGSLSAWVRLHYVYPYPHIDEVMQLMADGKILPYLDIPFQHASPK
                     VLKAMRRPGDQEKTLDRIKSWRTACPELALRSTFIVGFPGEADEDFELLLTGLTEARL
                     DRVGAFKYEPVGGAPANDLGLSPVPADVAEQRYRRLMEHQQAISANLLKRKVGRHIQA
                     IVDKAGPTVAEARSKADAPEIDGKVHIASRRPLRPGDIVQVKIEPADAYDLFGTTA"
     gene            complement(1485..1772)
                     /locus_tag="VGB93_01250"
     CDS             complement(1485..1772)
                     /locus_tag="VGB93_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144787.1"
                     /translation="MMAMKKMRVLSRFAAALLIAVAPMGAAFGQTPSAAKPNTAIPEK
                     QHLGPVNPPHAGVVKPTHDTDPDMTKKPQPQDPAETPVIPPSATPGGSEAK"
     gene            complement(1835..2500)
                     /locus_tag="VGB93_01255"
     CDS             complement(1835..2500)
                     /locus_tag="VGB93_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0144788.1"
                     /translation="MFFGIDFHPLIVRHGYAVVFFVVLLEGAGSPLPGETALVLAAVY
                     AGATGRLDIATVLLVAVAGGILGGTVGYWIGRSVSSEFLRKYGKWIGLTENRLALGRH
                     LFQSHGGKIVFFGRFIAVLRIVAALLAGLNQYDFKRFFLFNAAGVVAWAAIMGLGGYF
                     FGDAMTRVSGPLGAIGLAFAAAVVVGFLIVLRRQEKKFESKLSENAFAKDGEVKLETT
                     EPS"
     gene            2660..2956
                     /locus_tag="VGB93_01260"
     CDS             2660..2956
                     /locus_tag="VGB93_01260"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEY0144789.1"
                     /translation="MQVVSANRLSDGTVVYLNSQGNWGESLASATVFATPADVEAGLR
                     QAQAAIAANEVVDAFAVPVEQGVDGLHAVSLRNAIRELGPTIAFKTSPTAATRS"
     gene            2958..4616
                     /locus_tag="VGB93_01265"
     CDS             2958..4616
                     /locus_tag="VGB93_01265"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY0144790.1"
                     /translation="MYRYDDFDAAFVAERVEQFKDQVARRLSGELTEEQFRPLRLMNG
                     VYLQLHAYMLRVAIPYGTLSPRQLRMLGHIARRYDKGYGHFTTRQNIQYNWPALADCP
                     DILNDLASVEMHAIQTSGNCIRNTTSDQFAGAAADEIEDPRPYCELLRQWSSLNPEFS
                     YLPRKFKIAVSGAPHDRAAIQVHDIGLQVVRDAAGEIGFAVYVGGGLGRSPFIAHKIR
                     DFLPNADLLNYVAAILRVYNLEGRRDNKYKARIKILVHEKGADAMREAVEAEFAISQD
                     PDLVLTTDIIERFKSYFVLPDLPPRPVPAQNFEGRRAQDAEFAHFVAQNVVAHKVPGY
                     GVASISLKPIGGIPGDITAEQMEAVADLAETYAHNEIRVAHEQNLVLAHVALDDLAAI
                     YDRLKDIGLATANIGLVTDIIACPGLDYCALATARSIPIAQRISERFGQNGRAADIGE
                     LHINISGCINACGHHHVANIGILGLEKKGVETYQITLGGSADQNCALGEVLGPGFSSE
                     TIVDAIETIVDTYLKVRKNRDEDFLSAYQRVGVAPFKEAVYEHA"
     gene            4603..5340
                     /locus_tag="VGB93_01270"
     CDS             4603..5340
                     /locus_tag="VGB93_01270"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY0144791.1"
                     /translation="MSTPNGLLFGDQVAASRLSDRFYALGAEKLLRLAIEDLFPGRIA
                     LVSSFGAESAVLLHLISTIDPATPVLFLDTLHLFSETLEYRDALVTRLGLANVIGLKP
                     DERQLAEEDPETFLWSSNPDRCCAIRKVEPLAKALKNYDAWISGRKRFQAATRAALPL
                     FETDGERIKLNPLAAWTPEDIKAYFKKYDLPPHPLVAKNYLSIGCIPCTSPVRPGEDT
                     RAGRWRGRGKTECGVHVPATLEAGADI"
     gene            5343..5852
                     /locus_tag="VGB93_01275"
     CDS             5343..5852
                     /locus_tag="VGB93_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY0144792.1"
                     /translation="MALYRDGKFESDSWHRLEADELPEEGHVLLSLAEWRRFKAEQKN
                     SNIAFGVMLEPGETAEMIAEDLPRLALVAVSFPKFTDGRGYSTARILRDRYKFAGELR
                     AVGDILFDQFQLYGRSGFDSLEIKDAATIKLLESGRKPVMTHFYQPGEGPEIKDSKSP
                     WRRRSTSQA"
     gene            complement(5803..6156)
                     /locus_tag="VGB93_01280"
     CDS             complement(5803..6156)
                     /locus_tag="VGB93_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144793.1"
                     /translation="MSIDEIVHTCSNENVARAAVASIGVSFALRVRAAADLLGVSTGL
                     FAARAVRTFAAAAPIDERCEVVRAMFRADQPILRGLEAILERELDDVSSLDSREWRPV
                     TPAMSSDAARDFCCP"
BASE COUNT         1310 a         1992 c         1793 g         1274 t
ORIGIN      
        1 cgaagtactg agaactgctt taggaaatcc aagaacccgg ccctttgtgg ccgggttttt
       61 tcgttgtgcc gcctcccggc cctgcagttc cgattcacgc ccttgcggcc gcgagcgaga
      121 catggcactg cacggccatg acgcgcgagc catcggcagc gactacggcc gccagccctc
      181 ccaaaatctc cttcgtttct ctcggttgtc ccaaagcttt ggtcgacagc gagcgaatca
      241 tcacgcggct gcgcgccgag ggctatgagc tgacccgtag ccatgccggc gccgacgcgg
      301 tgatcgtgaa cacctgcggt tttcttgata gcgccaaggc cgaatcgctc gcggcgatcg
      361 gcgcggcact tgaagaaaac gggaaagtca tcgtcaccgg ctgtatgggt gccgaatcgg
      421 agacgatccg cagcgcctat cccaatgtcc tcgcgatcag cggcccgcaa gcctacgaaa
      481 gtgtcgtcgg cgccgtccat gatgcgatcg cgccgccgca cgatcccttc ctcgatctcg
      541 ttccagcaca gggcatcaag ctcacgccgc gccattacgc ctatctgaag atttccgagg
      601 gctgcaacaa ccgctgctcc ttctgcatta ttccgcggct gcgcggcgat cttgtctcac
      661 gccccgccgc ggacgtgctc cgcgaagccg aaaaactcgt cgccgccggc gtcaaagagc
      721 tgctggtcat ctcccaggac acgggcgcct atgggcttga cctgcgctat gcggagagca
      781 cgcttggcga tcgacaggtg cgggccaaat tcatcgatct cgcgcgcgaa ctcggcagcc
      841 tcagcgcctg ggtacggctg cattacgtct atccctatcc gcatatcgac gaggtgatgc
      901 agctgatggc ggacggtaag atattgccct acctcgacat tccgttccag cacgcgagcc
      961 cgaaggtttt gaaggcgatg cggcgccctg gcgaccagga aaagacgctc gatcgaatca
     1021 agtcctggcg caccgcctgc cccgaactcg ccttgcgctc gacctttatc gtcggctttc
     1081 ccggcgaggc ggacgaggat ttcgagcttt tgcttactgg gctgaccgag gcgcggctcg
     1141 accgggtcgg cgcgttcaaa tacgagccgg ttggcggcgc gccggccaat gatctcggac
     1201 tatctccggt gcctgcggac gttgctgaac agcgctaccg gcgcttgatg gaacatcaac
     1261 aggcaatcag cgcgaatctg ctgaagcgca aagtcggtcg tcatattcaa gcgatcgtcg
     1321 ataaggccgg cccgacggtt gccgaggccc gcagcaaggc tgacgcgccg gagatcgatg
     1381 gcaaggttca catcgccagc cgccgcccgc tacgcccggg cgatattgtt caggtcaaga
     1441 tcgagccggc cgacgcttac gatctcttcg gcacgacagc gtagctattt cgcctcgctg
     1501 ccgcctggcg tcgccgaggg cggaatcacc ggcgtttccg caggatcctg tggctgtggc
     1561 tttttagtca tatcgggatc agtgtcatgc gtcggcttca ccacgccggc atgcggcgga
     1621 tttactgggc cgagatgctg tttttcggga atcgccgtat tcggcttcgc ggcggagggg
     1681 gtctgcccga aggccgcgcc catcggcgcc accgcaatca gcaaagccgc agcgaatcgc
     1741 gaaagcactc gcatcttttt catggccatc atcggcgcct cctttgggag cgaacgcctt
     1801 tgaggcatgg ctgttccagc gcaaccgagg ctgttcagga gggctcggtt gtttccaatt
     1861 tcacttcgcc gtctttcgcg aaggcatttt cggagagttt cgattcgaat tttttctcct
     1921 gccggcgcag gacaatcaga aacccgacaa cgacagccgc cgcgaacgca aggccaatcg
     1981 cgcccaacgg gccactgacg cgagtcatcg cgtcgccgaa aaaataccca ccaagaccca
     2041 tgatcgccgc ccaggcgacg acgccagcgg cgttgaacag aaaaaaccgc ttgaaatcat
     2101 attggttgag gccagcaagc agcgccgcga cgatacgcag aacggcaata aaccggccga
     2161 agaagacgat cttgccgccg tggctctgaa acaggtgtcg ccccagcgcc aatcgattct
     2221 cggtcaatcc gatccacttt ccgtatttgc gcaaaaattc gctgctgacg ctgcgcccga
     2281 tccaatagcc gaccgtcccg ccaaggatgc cgccggccac agcgaccagc agaaccgttg
     2341 cgatatcgag ccttcccgtg gccccggcat agaccgcggc caaaacgagc gccgtttcac
     2401 ccggaagcgg cgagcccgcc ccttcgagca gaacgacaaa aaagaccacg gcatatccgt
     2461 gccggacgat cagcggatgg aagtcgatcc caaaaaacaa aatgcctccc agggacacga
     2521 attctgcccc agttcatgag tccagcaaga attacgattt cagaatgtta tatagatcaa
     2581 ttgtcacaaa tttctggcga aattcgccag catgacctat atcccactcg cgttcacttt
     2641 tttgggaaaa tgcagcgtga tgcaagtcgt ttcggccaac cggcttagcg atggcaccgt
     2701 cgtctacctc aattctcaag ggaattgggg tgaatcgctg gcatccgcaa ccgtcttcgc
     2761 cacaccggcg gatgtcgagg ctgggcttcg ccaggcgcaa gccgccattg cggccaatga
     2821 ggtcgtcgac gcctttgccg taccggtgga gcaaggcgtg gatgggcttc atgccgtcag
     2881 tttgcgcaat gccatccgcg aactcggccc gacgatcgcg ttcaagacat cgcccacagc
     2941 ggcgacgcgg agctgacatg tatcgctacg acgacttcga cgcagccttc gtggccgagc
     3001 gggttgagca gttcaaggat caggttgcac gccgtctgtc tggcgaactg accgaagagc
     3061 agtttcgccc gttgcggctg atgaacggcg tctatctgca gctgcatgcc tatatgctgc
     3121 gcgtcgcgat tccctacggc accttgtcgc cgcgccagct tcggatgctc ggccatatcg
     3181 cgcgccgcta tgacaagggt tacggccatt tcacgacccg gcaaaatatc cagtacaact
     3241 ggccagcact ggccgattgc cccgatattc tcaatgacct tgccagcgtc gaaatgcacg
     3301 ccattcagac gtcgggcaat tgtatccgca atacgacgtc ggatcaattt gcaggcgccg
     3361 ctgcggacga gatcgaagat ccccggccct attgcgagct tctgcgccaa tggtcatcgc
     3421 tcaatccgga attctcctac ctgccgcgta aattcaagat cgcggtttcg ggcgcgccgc
     3481 acgaccgcgc tgcgattcag gtgcatgaca tcggcttgca ggtcgtccgc gacgccgcgg
     3541 gcgagatcgg attcgcggtt tatgtcggcg ggggccttgg tcgttcgccc ttcattgccc
     3601 acaaaatccg cgacttcctg ccgaacgccg accttctgaa ttacgtcgcc gcgattctgc
     3661 gcgtgtacaa tctcgaaggg cgccgcgaca acaaatacaa ggcacgcatc aaaatcctcg
     3721 tccacgaaaa gggcgccgac gcgatgcgcg aggcagtcga ggcagaattc gcgatcagcc
     3781 aggatcccga tctcgttctc accacggata tcatcgaacg attcaagagc tatttcgtcc
     3841 ttcccgattt gccgccgcgg cctgtgccgg cgcagaattt cgaggggcgc cgtgcgcagg
     3901 acgccgagtt cgcgcatttc gtcgcgcaga atgtcgtcgc acataaggtg ccgggttacg
     3961 gcgtcgcgtc gatctcgctg aaaccgatcg gcggcatccc cggcgacatc accgccgagc
     4021 agatggaggc ggtcgccgat ctcgcagaaa cctatgcgca caacgaaatc cgcgtcgcgc
     4081 atgaacagaa tctcgtcttg gcgcatgtcg cgctcgacga tttggcggct atctatgaca
     4141 gattgaaaga tatcggcctc gcgacggcca acatcggcct tgtcaccgac atcatcgcct
     4201 gccccggcct ggactattgt gcgctcgcaa cagcgcgctc gatcccgatt gcgcagcgca
     4261 tttccgaacg tttcggacag aatggccgcg ccgccgatat cggcgagttg cacatcaata
     4321 tatctggctg catcaacgcc tgcggccacc atcacgtcgc caatatcggc attctcggcc
     4381 ttgagaaaaa aggtgtcgaa acctatcaga tcacgcttgg cggctccgcc gaccaaaact
     4441 gcgcccttgg cgaagttttg ggcccgggct tttcgagcga aacgattgtc gatgcgatcg
     4501 agacgatcgt cgacacctat ctcaaggttc gtaagaatcg cgacgaagat tttctctcag
     4561 cctatcaacg cgtcggcgtc gcgcccttca aggaagcggt ctatgagcac gcctaacggc
     4621 ctgctgtttg gcgatcaggt tgcggcctcg cgcttgtccg atcgctttta tgcgctcggc
     4681 gcggagaagc ttttgcggct tgcgatcgag gacttgtttc ccggccggat cgcgcttgtt
     4741 tcgagcttcg gcgccgaatc tgcggtgctt ctgcacttga tctcgacgat cgatcccgcg
     4801 accccggttc tcttcctgga caccttgcat ctgttttctg aaacgctcga ataccgtgat
     4861 gctctcgtca cacgtcttgg cctggcgaac gtcatcgggc tgaaaccgga cgaaaggcaa
     4921 ctggccgagg aagacccgga gactttcctc tggtccagca accccgatcg ctgctgtgcg
     4981 attcgcaaag tcgaaccctt ggcgaaggcg cttaaaaatt acgacgcgtg gatcagtggc
     5041 cgcaaacgct ttcaggcagc gacgcgcgcc gcattgccat tgtttgagac cgacggcgag
     5101 cgcattaagc tcaatccgct cgcagcctgg acgcccgagg acataaaagc ttatttcaaa
     5161 aaatacgacc tgccgccgca cccgttagtc gcgaaaaatt acctgtcgat cggctgcatt
     5221 ccctgcacct cgccggtccg ccccggcgaa gacacgcgcg ccgggcgctg gcgcggtcgt
     5281 ggcaaaaccg aatgcggcgt gcatgttccc gcaactctcg aagccggtgc ggatatctga
     5341 ttatggcgct ttatcgcgac ggaaaattcg agagtgattc ctggcacagg cttgaggcgg
     5401 acgaactccc cgaagagggt catgtgctgt tgagcctcgc tgaatggcgg cgatttaagg
     5461 cggaacagaa gaacagcaat atcgcctttg gcgtcatgct cgaacccggc gaaacagcag
     5521 agatgattgc cgaggatttg ccgcggctag cgctcgttgc ggtgagtttc cccaaattca
     5581 ctgatgggcg gggttattcg acggcgcgaa tcctccgcga ccgctacaaa ttcgccggcg
     5641 aactccgtgc cgtcggcgat atcctcttcg atcaatttca gctttatggt cgttctggct
     5701 tcgattcact cgaaatcaaa gacgccgcaa cgatcaaatt gctcgaatcc ggccgcaagc
     5761 cggtgatgac gcatttctat cagcccggcg aagggccaga gatcaaggac agcaaaagtc
     5821 cctggcggcg tcgctcgaca tcgcaggcgt gacaggtcgc cactcgcgac tatccagcga
     5881 actgacatca tccaattcgc gttccaggat cgcctcaagt ccgcgcagga tcggctggtc
     5941 agcgcgaaac atcgcccgca cgacttcgca tcgctcgtcg atgggcgccg ctgcggcaaa
     6001 cgtacgtaca gcgcgcgcag cgaataggcc ggtgctgaca cccaaaaggt ccgccgccgc
     6061 tcggactctc aaggcaaagg aaactccgat cgaagccaca gccgctcgcg caacattctc
     6121 gttcgagcat gtgtgaacga tttcatcgat cgacattgaa gcctccttgc ctatgtctct
     6181 tgcaaactcg acgaaacatc cactgtgatg ccgtcctgcg tttgcgacac tttagactct
     6241 ttctgaattt gaaagagtct agttcatcgc tgccgtttcc aagtccgcat cttcgtcatc
     6301 ggcggcgaca aaaaaccaat gacggtcgaa ctcggcaaac tcgtgacggt aacgcgattc
     6361 tttcgtgtt
//
LOCUS       DASWOG010000065         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_313135,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000065 DASWOG010000000
VERSION     DASWOG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313135"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGB93_01285"
     CDS             complement(<1..506)
                     /locus_tag="VGB93_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0144794.1"
                     /translation="MSDPVSAPAASVAASLSPIDLFLHADPIVKGVIILLVLASAWGW
                     AIIVDKLIRLSVLNRRATKLIASVKSGLPVSTIAESFAGASEKDPFVAVYHSMVEENR
                     RSADIIHTEAQTDSLQDRVHRVAQLASLGALEHLQNRLPNLATIGAVAPFVGLFGTVW
                     GIMNSFQG"
     gene            complement(532..1362)
                     /locus_tag="VGB93_01290"
     CDS             complement(532..1362)
                     /locus_tag="VGB93_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0144795.1"
                     /translation="MTSALAPQLNFFPTSGEAAVAEDDGAHSLLRSHSFRPAYIAQGG
                     AILVYALALAGLCYLAKPKPVPQDQPIELTMVQDDAPAQLDTPDTTPTPPATAEQTPT
                     PPDSPPVPDAPPPDQTALATPDTPPPDQNLPPPPVDQQETVAPVAPPPPPVAVKRYHQ
                     PKAVAARTTNSRHFASAPASASGSPSAAPSYYANEVHARIASVASDTVPDDFHGSGRV
                     SYHIVIGPSGELISKSISSSGNALFDRAAAEALGRAAPFPPTGMGRPVSLHGAIAFSQ
                     "
     gene            complement(1544..>1888)
                     /locus_tag="VGB93_01295"
     CDS             complement(1544..>1888)
                     /locus_tag="VGB93_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0144796.1"
                     /translation="CEGVKAVPPQLKEVGRVYCLSPWLLFRRIILPAATPAIVTSLQL
                     SLLLAWICTIGAEYLMGGMAQGIGTFVMMGEDQVRPDIILLGVSVIAASGFAFNKFVR
                     STSRYLLRWRQA"
BASE COUNT          400 a          529 c          595 g          364 t
ORIGIN      
        1 atgccctgga acgagttcat gatgccccac acggttccga agagaccgac aaagggggcg
       61 acggcgccga tcgtcgcgag attgggaagc ctgttctgca aatgctcaag cgcacccaaa
      121 ctggcgagtt gcgcaacccg gtgcacacga tcttgcagac tatccgtttg ggcctcggtg
      181 tggatgatgt ccgccgagcg ccggttctcc tcgaccatcg aatgatagac tgcaacgaaa
      241 ggatcttttt ccgaagcgcc ggcgaaactt tcggcaatcg tcgacactgg aaggccggac
      301 ttgacgctgg cgatgagttt cgtcgcgcgc cggttcagca cgctgagccg aatgagcttg
      361 tcgacgatga tcgcccagcc ccaagccgaa gccaggacca acaggatgat aacgcctttg
      421 acgatcggat cggcgtgaag aaacaaatcg attggcgata ggctggcggc gacggaggcc
      481 gccggcgcgc tgacaggatc tgacatggaa tcttccttcc ttaagggatt gctattggct
      541 gaaggcgatc gccccatgga gggacactgg ccgccccatg ccggtcggcg ggaacggcgc
      601 ggcacgcccc aaagcctcgg ccgcagcgcg gtcgaaaagc gcatttcctg atgacgagat
      661 gcttttcgaa atgagctcgc ccgagggccc gatcacgata tggtaggaaa cgcgcccgct
      721 gccgtgaaaa tcatccggca ccgtgtcgct ggcgacgctg gcgatacgcg catggacctc
      781 gttggcgtaa taggaaggcg cggcgctggg gctaccgctc gcagaggcag gtgctgatgc
      841 aaagtggcgt gagttcgtag tccgcgcggc aacggctttc ggctgatgat agcgtttgac
      901 cgcgacgggc ggtggcggcg gcgcaacggg agcaaccgtc tcctgttgat cgacaggcgg
      961 gggcggcaga ttctgatccg gaggcggcgt atcaggcgtc gccagggccg tctggtcagg
     1021 cggcggcgcg tcggggacag gaggcgaatc cggcggggtc ggcgtctgtt cggctgtcgc
     1081 gggcggagtg ggtgtcgtgt ccggcgtatc gagctgggcc ggcgcgtcat cctgcaccat
     1141 ggtcagttcg atcggctgat cctgcggtac cggcttcggc ttcgccagat agcacaatcc
     1201 cgcaagcgca agcgcataaa cgagaatcgc gccaccctgc gcaatgtaag caggacgaaa
     1261 cgagtgcgat ctcagaagcg aatgcgcgcc atcatcttcc gcaaccgcgg cttcgccact
     1321 ggtgggaaag aagttcaact gcggcgccaa agctgacgtc atggcttgcc tttcacgtat
     1381 cgattttgaa ttcgcgtaga ggatagcgtt taatatccat aaactctacc tattttatat
     1441 ggaattggtc ttcaaatttc cttggctggc tcgactcagc gtttccgtcg tcagtacgga
     1501 atgttgctca acgattcagt tgagaggaac gatctggcga ggtctatgcc tgacgccagc
     1561 gcaaaagata acgtgacgtc gagcgaacaa atttgttgaa tgcaaatccg gatgccgcaa
     1621 tgacgctgac gccgagcaag atgatatctg gcctgacttg atcttctccc atcatgacaa
     1681 acgttccgat cccctgcgcc attccgccca tcagatattc ggcgccaatc gtgcatatcc
     1741 acgcgagcaa aagcgatagc tgcaaggatg tgacgatggc cggcgttgcg gcgggaagga
     1801 tgatcctgcg gaaaaggagc caggggctta gacaatagac ccggcccact tccttcaatt
     1861 gcggcgggac ggctttgacc ccttcgca
//
LOCUS       DASWOG010000066         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_111531,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000066 DASWOG010000000
VERSION     DASWOG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111531"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="serB"
                     /locus_tag="VGB93_01300"
     CDS             complement(<1..868)
                     /gene="serB"
                     /locus_tag="VGB93_01300"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0144797.1"
                     /translation="MLSVATLVSPPALAALDDRTLQNVARSIPGVEQTAWLDAGIAAD
                     IFFDAPASAGLLSALRAGLAETRIDVFVQPVAHRRKKLLLADMDSTMIEQECLDELAD
                     FAGLRDHVAPITARAMRGEIAFEPALRERVALLEGLGIGVIDKIGAHRVTLTPGAKTL
                     VATMRAHGAFSVLVSGGFTAFTEKIAAMIGFDDHVANALGVAGGRLTGRVEEPILGRA
                     AKREILEAFCRKLDISAEETLAVGDGANDLDMLEGAGLGVAFHAKPAVAAAAQARIDH
                     CDLTALLYAQGYR"
     gene            943..1287
                     /locus_tag="VGB93_01305"
     CDS             943..1287
                     /locus_tag="VGB93_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144798.1"
                     /translation="MKRRICIFIFLMLFASATRASSCGAQTDKVQARVDARLDAVAGT
                     DPGARESRAALLHHEPTPASIAAAERGLKGQHGVERALAALKRARKAEARGDIKGCEA
                     ALVEARAAIAPR"
     gene            1740..2951
                     /locus_tag="VGB93_01310"
     CDS             1740..2951
                     /locus_tag="VGB93_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0144799.1"
                     /translation="MSNKPRSPGRHFLQIPGPTPIPDRVLRAIDQPVVDHRGPVFQKL
                     SKRVLEKVKAPFKTKNPVIIFPSSGTGGWEAALVNTLSPGDKVLMFETGQFASLWNTM
                     AKSLGLKPEFIESDWRIGADPAQLKERLKADKNHEIKAVCIVHNETASGTTSPLQEIR
                     AVLDELKHPALYFVDAVSSLASIDLRHDEWGIDVTIAGSQKGLMLPAGLGFNAVSQKA
                     LEASKKSTFPKSYWSWADMLGANASGFFPYTPATNLLYGLDVALDMLEEEGYDRVFAR
                     HKRHAEATRRAVVAWGLEVLCKDPKYYSPVVTSVLLPPGHDADAFRALALDHFDISLG
                     TGLGRVAGKVFRIGHLADTNDLTILGALSGVEMTLELAGIPHKKGGVQAALNYIAEQT
                     RDHSHKLAAAE"
     gene            3109..>4426
                     /locus_tag="VGB93_01315"
     CDS             3109..>4426
                     /locus_tag="VGB93_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0144800.1"
                     /translation="MGAQIANQDDIFAFLASPATYGFDQSQQNSIKRIDTHGAVVFLA
                     GTEAYKVKRAVFFPFMDFSTLEKRRAACENEIRVNRENAPDIYLGTVPIRRTDKGLRL
                     GGEEGEIVEWAVHTRRFDDESTLDKLAARGALDPAVAADLAESISTAHARADRSDLAT
                     TQRFHKWMIDTFDGLADAPIFAPDDVAALRSSFLAAYSATAALLKRREAQGKVRRCHG
                     DVHLRNIVLENGKPILFDAIEFDDLLATIDILYDLAFVLMDFWQRGLHAHANLLFNRY
                     LWRAPELEQELEGLAALPMFLALRAAIRARVAAALSDIAAADHSAEVKSFFAAAVAFL
                     RPESARLVAVGGLSGTGKSTLAAALANAIGRAPGAVQLRSDIERKRLRTAGEYERLSA
                     DAYAPEVTARVYDRLHNLAEIALRSGQSVIVDAVHARKDERGALTTH"
BASE COUNT          826 a         1345 c         1383 g          872 t
ORIGIN      
        1 gccggtagcc ttgtgcgtag agcagggcgg tgaggtcgca atgatcgatg cgggcttgcg
       61 ctgccgccgc gaccgcgggt ttggcatgga aggcgacgcc caatcctgca ccttcgagca
      121 tatcgaggtc gttggcgcca tcgcccaccg cgagcgtttc ttcggctgaa atatcgagtt
      181 tccggcaaaa cgcctccaga atctcgcgct tcgccgcgcg gccaaggatt ggttcttcga
      241 cacggcccgt caatctgccc ccggcaacgc cgagggcatt ggccacatgg tcgtcaaagc
      301 cgatcatcgc ggcgattttt tcggtgaagg cggtgaaccc accggagacg agaacgctaa
      361 aggcgccgtg cgcgcgcatc gtggcgacga gcgttttagc gccgggggtc agcgtaacgc
      421 ggtgggcacc gatcttgtcg atcacgccaa tgccgaggcc ttccaggagc gcgacccgct
      481 cgcgcaacgc cggttcgaaa gcaatttccc cgcgcatggc gcgggcggtg atcggcgcga
      541 cgtggtcccg cagcccagca aaatcggcaa gttcgtcgag gcattcctgt tcgatcatcg
      601 tcgagtccat atcggcgagg aggagttttt tgcgccggtg ggcgacgggc tgcacgaaga
      661 cgtcgatgcg tgtctctgca agtccggccc gcagcgcgct taggagtccc gccgaggcgg
      721 gcgcgtcgaa gaagatgtcg gcagcgatgc ccgcgtcgag ccacgcggtt tgctcgacac
      781 cgggaattga ccgggcaaca ttctgcaaag tccggtcatc caaagccgcc agggcgggag
      841 gggagacgag ggtagcgaca ctcagcatgg ggttctataa attccggcgg cgccagcttt
      901 ttcggcgaaa ttttgctacg ctaagacctt aagggttccg cgatgaaacg gcgaatttgc
      961 atcttcattt tcctgatgct ctttgcatcc gcaacgcgag cgtcctcgtg cggcgcgcag
     1021 accgacaagg ttcaggcgcg ggtcgatgcc cgcctcgacg ccgttgcggg taccgatccg
     1081 ggcgcgcgtg aatcccgggc ggcgctttta catcacgagc cgacgccggc ctctatcgct
     1141 gccgccgagc gtggtttgaa gggacaacac ggtgtcgaac gggcactggc ggcgctgaaa
     1201 cgcgcgcgga aagccgaagc gcggggcgac atcaaaggtt gcgaagcggc gctcgttgaa
     1261 gcccgcgcgg cgatcgcacc gagatagcgc gcgcccaacc acgttttcgg aggctgggta
     1321 aacgtccggg tctgcgcgtc aaatgagcac gggtgaggaa atccgcctca tgacacgcca
     1381 cggcttcaga taaaaatcga gcgccaaagt gcgtttcccg agggtgctgc ggggcgaaat
     1441 tttctattgc agcgcacacc ggacgtgttt gccaaaatac cagatttgtg ttgagccgcg
     1501 ttctttttga tcttattcat gcgccaaccg cgacggcgcc ggtgggatgc gcgggaattt
     1561 ctgcatccgt cggcgccttc tcgagtcctt actcgatgat caaatttttc tcgtcggttg
     1621 cgccggccgg gaaaatgggc gcgaggggtc tgcgcacgcg ggcctgccta aaaataaacc
     1681 acgaagcatt tgtccgttgt ttgggcaaag gcggcatttg agatttggag aactagacaa
     1741 tgagcaacaa gccgcggagc cccggccgcc atttcctgca aattccaggc ccgacgccga
     1801 tcccggatcg cgtgctgcgc gcgatcgatc agccggtcgt cgatcatcgc ggtccggtgt
     1861 tccagaagtt gtcgaagcgg gtgctcgaaa aggtcaaggc tcccttcaag accaagaatc
     1921 cggtcatcat tttcccgtcg tcaggcaccg gcggctggga agctgccctc gtcaataccc
     1981 tgtcgcccgg tgacaaggtg ctcatgttcg aaactggcca gttcgcgtcg ctgtggaaca
     2041 cgatggcgaa gagcctgggc ctcaagccgg agttcatcga atccgattgg cgcatcggtg
     2101 ccgacccggc tcagctcaag gaacgcctca aggccgacaa gaaccacgag atcaaggcgg
     2161 tctgtatcgt ccataacgag acggccagcg gcacgacctc gcctttgcag gaaatccgtg
     2221 cggttctcga cgaattgaag catccggcgc tgtacttcgt tgatgctgtc tcatcgctcg
     2281 cgtcgattga cctgcgtcac gacgaatggg gcatcgacgt caccatcgcc ggctcgcaga
     2341 agggcctgat gctgccggcg ggccttggct tcaacgcagt gagccagaag gcgctcgaag
     2401 cctcgaagaa gtccaccttc ccgaagtcct attggtcctg ggctgacatg ctcggcgcca
     2461 atgccagcgg cttctttcct tatacgccgg cgacgaacct gctttacggc ctcgacgttg
     2521 cgctcgatat gcttgaagag gagggctacg atcgcgtttt cgcccggcac aagcgccatg
     2581 cagaagcgac ccgccgcgcc gtggttgcgt ggggtctcga agtcctctgc aaggatccga
     2641 agtactattc gcctgtcgtg acctcggtcc tgttgccgcc gggccatgac gccgatgcgt
     2701 tccgtgccct cgcgctcgat catttcgaca tttcgcttgg caccggcctt ggccgcgttg
     2761 cgggcaaggt gttccgcatc ggtcatcttg ccgataccaa cgacctgacg atcctcggcg
     2821 ctctcagcgg tgtcgagatg acgcttgagc ttgctggtat tccgcacaag aagggcggcg
     2881 tccaggcggc gctcaactac atcgctgagc agacgcgcga tcattcgcac aagctcgcag
     2941 cggcggaata attttctcgt cttgtcgggc gggtgaaaat ccgtccggac ttttttgaaa
     3001 cattccgggc ggcgcatatg tgccgcccga attttttgga atattgaggt tggcattcgg
     3061 tgccggccgc tcccgtccgg cacttcatgc tttacgtgaa gagcagccat gggcgcgcaa
     3121 attgccaatc aggacgacat attcgcgttt ctcgcgagcc ccgcgaccta cggctttgat
     3181 cagtcacaac aaaattcgat aaagcgcatc gacacgcacg gcgccgtcgt gtttctcgcg
     3241 ggcactgagg cctataaggt caagcgcgcc gtcttctttc ctttcatgga tttctcgacg
     3301 ctggaaaagc gtcgcgcggc ctgcgaaaac gaaattcgcg tcaatcgcga gaatgcgccc
     3361 gacatttatc tcgggaccgt gccgatccgc cgcacagaca aggggctgcg cctcggcggc
     3421 gaagaaggtg aaatcgtcga atgggcggta catacgcgcc gcttcgatga cgagagcacg
     3481 ctcgacaaac ttgctgcgcg cggggcgctc gatccggccg tcgcggccga ccttgccgaa
     3541 agcatttcca ccgcgcacgc acgtgcggac cgcagtgatc tcgcaacgac gcagcgattt
     3601 cataaatgga tgatcgacac gttcgacggt ctcgccgacg cgccgatttt tgcgccggat
     3661 gatgtcgcgg ctctcagatc gagctttctt gcggcctatt ccgccaccgc cgcgttgctc
     3721 aaacggcggg aggcgcaagg gaaggtgcgg cgctgccatg gtgacgttca cctgcgcaat
     3781 atcgttctcg aaaacggcaa gccgatcctg ttcgacgcca tcgaattcga cgacctgctg
     3841 gcgacgatcg atattcttta tgaccttgcc ttcgtactga tggacttctg gcagcgcggc
     3901 ctccacgccc atgccaatct cttgttcaac cgctatttgt ggcgcgcgcc tgaacttgag
     3961 caggagcttg agggactggc cgcgctgccg atgtttctgg cattgcgcgc ggcgatccgc
     4021 gcgcgcgtcg ccgcggctct ctccgacatc gccgccgcgg atcacagcgc tgaggtgaag
     4081 agctttttcg cggcggcggt ggctttcctg cggcctgagt cggcccggct tgtggcggtc
     4141 ggtggtttgt cggggaccgg caagagcaca ctcgccgcgg cgcttgccaa tgctatcggc
     4201 cgggcgccgg gcgcggtgca actacgcagc gacatcgagc gcaagcggct ccgcaccgcc
     4261 ggcgaatatg aaaggctatc tgcggacgcg tatgcgccgg aggtcacggc gcgggtttat
     4321 gaccgtctgc acaatctggc cgagatcgcc ctacgctctg gccaaagcgt cattgtcgat
     4381 gcggtccacg caagaaagga cgagcgcggc gcattgacga cacacg
//
LOCUS       DASWOG010000067         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_258180,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000067 DASWOG010000000
VERSION     DASWOG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258180"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="sthA"
                     /locus_tag="VGB93_01320"
     CDS             complement(<1..1123)
                     /gene="sthA"
                     /locus_tag="VGB93_01320"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY0144801.1"
                     /translation="MYDYDMVVIGSGPSGRRAAVQFAKLGKSVLVVEKGRRVGGVSVH
                     TGTIPSKTLRETVLNLSGWRERGFYGRSYRVKQDIGAVDLIARLQKTLDHEVEVLEHQ
                     FSRNMVKTACGEARFIGPNQIEIAADGGEIRNVSAAHILIACGTRPFRPEYVPFDNKS
                     VFDSDEIIDLPKLPRSLAVIGAGVIGVEYATIFSALDVAVTLIEPRPSFLDFIDKELI
                     EEFMHELRDRNVSMRLGSAVNSIERSEGGSIVTRLADGRNVVSEMLLFAAGRVGATDR
                     LNLEAAGIAVDHRGRIAVDPLTLQTSVPHIYAAGDVIGFPSLASTSMEQGRVAACHAL
                     GMEPMAPPEFFPYGIYSVPEISTTGLTEEEVRTRGIPYEV"
     gene            complement(1116..1328)
                     /locus_tag="VGB93_01325"
     CDS             complement(1116..1328)
                     /locus_tag="VGB93_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144802.1"
                     /translation="MTVSTCAGRVQVDGDSRHSELMTNDIDAKHGLRGDGDREHSRLF
                     GPIAAWKMASGLLSSTSSAFGTAFDV"
     gene            complement(1370..>1575)
                     /locus_tag="VGB93_01330"
     CDS             complement(1370..>1575)
                     /locus_tag="VGB93_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144803.1"
                     /translation="DQIGSALQSKKTLCCLSPEMPEIVIAIVNGRQVPRRAASTSEMR
                     RKWQSTQQIAVAEYAMRFHDPLD"
BASE COUNT          303 a          496 c          461 g          315 t
ORIGIN      
        1 cgacctcata gggaatgccg cgggtccgca cttcttcttc ggtcagaccc gtcgtcgaaa
       61 tttccggcac cgaataaatc ccgtagggaa agaattccgg cggcgccatc ggctccatgc
      121 cgagcgcatg gcaggccgcg acccgcccct gctccatcga ggtagacgcc aggctgggaa
      181 atccgatcac gtcgccggcg gcataaatat gcggcacgct ggtttgcagt gtgagcggat
      241 cgaccgcaat ccggccgcgg tgatcgaccg cgattcctgc cgcttcgaga ttaagccggt
      301 cagtcgcgcc gacgcgtccc gccgcaaaca acagcatctc cgagacgacg ttgcggccat
      361 cggcaagccg tgtgacgatg ctgccgccct cgctgcgctc gatcgaattg acggcagagc
      421 cgagccgcat cgagacattg cggtcgcgca gttcgtgcat gaactcctcg atcaactcct
      481 tgtcgatgaa atcgaggaag ctcgggcgcg gctcgatcag cgtcaccgca acatcgagcg
      541 ccgagaagat ggtggcgtat tcgacgccga tcacgccggc gccgatcacc gcgaggctgc
      601 gcggcagttt tggcagatcg atgatttcgt cgctgtcgaa cacgctttta ttgtcgaacg
      661 ggacatattc cggccggaac ggccgcgtgc cgcaggcgat caggatatgg gctgccgaga
      721 cgttgcgtat ctcgccgccg tcagccgcga tctcgatctg gttcggcccg atgaagcgcg
      781 cttcgccgca ggcggttttc accatgttgc ggctgaactg atgttccagc acctcgactt
      841 catggtcgag cgtcttttgc agccgcgcga tcagatcgac ggcgccgatg tcctgcttga
      901 cgcgatagga tcggccataa aagccgcgct cgcgccagcc cgacagattg agcacggttt
      961 cgcgcagggt tttggagggg atagtcccgg tatggaccga aacgccgccg acccgccggc
     1021 ctttttcgac caccagaacc gacttgccga gtttggcgaa ttgcacagcg gccctgcggc
     1081 cggacgggcc ggagccgatc accaccatgt catagtcata catcgaaggc agtcccgaac
     1141 gcgctggaag tactggaaag aaggcccgag gccattttcc atgcagcaat cgggccaaaa
     1201 agacgtgaat gctcgcgatc accgtcgccg cgcaatccgt gtttggcgtc gatatcgttc
     1261 gtcatcaact cgctgtgccg actgtcgcca tcgacctgca cgcgcccggc gcaagtcgac
     1321 actgtcatcg ccttcgcgac gcggctttct ttcgcttttt tacaacccgt taatccaacg
     1381 gatcatggaa gcgcattgca tattctgcca ccgcgatttg ctgtgtcgat tgccatttgc
     1441 gccgcatttc tgacgtggat gcggcgcgcc tcggcacctg ccgcccgtta acgatagcga
     1501 tcacgatctc aggcatttct ggggaaagac agcacaaggt ctttttactc tgcagtgcag
     1561 aacctatctg gtcct
//
LOCUS       DASWOG010000068         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_184877,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000068 DASWOG010000000
VERSION     DASWOG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184877"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..761)
                     /locus_tag="VGB93_01335"
     CDS             complement(159..761)
                     /locus_tag="VGB93_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0144804.1"
                     /translation="MVIGRPREFDREAALDAAMHLFWRKGFATTSMKDLCDAMGIGSP
                     SLYAAFGCKETLYREAVARYLCTCGSPLWNKLTESPTAHAGVRSFLLAAAENFPESKT
                     TPAGCMAMLGAVSDEWPASIAEDVRNARLESLRMFRSRLEDAVAEGELPASADIDRLS
                     RFFLGIFQGMAIQARDGATRAELKGIAEAAMMAWPTDASD"
     gene            866..1603
                     /locus_tag="VGB93_01340"
     CDS             866..1603
                     /locus_tag="VGB93_01340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY0144805.1"
                     /translation="MTLAGKRALVTGASRGIGAAIAKALAAEGADVAITYEKSADRAA
                     EVVRAIKAQGRRAVAIQADSADAAPVRASVEKTVAELGGLDILVNNAGILRVSELKDI
                     SLDDIDAILDVNVRAPIVASKAAIPHLGKGGRVISIGSFFADRVPSPVLSVYAASKSA
                     LVGFTKGLAREVGPKGITVNLVQPGSIDTDMNPADGPFAETLRQMTAFGRYGTVDDIA
                     DAVAFLASPKAQYITGTTLTVDGGAIA"
     gene            1800..>2086
                     /locus_tag="VGB93_01345"
     CDS             1800..>2086
                     /locus_tag="VGB93_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0144806.1"
                     /translation="MGSSHFFLHDGQAGPQDAGAQSLPQVIVSTPLERQIDKELTFLG
                     QFSAVDRVELRAQVGGILTGIYFKDGAIVRKGDLLFTIDTVPYAIKLAQAQA"
BASE COUNT          436 a          648 c          596 g          406 t
ORIGIN      
        1 atgccagcga tgtggtccac cgtatcctga cggaccttcc cgagaattcg tcgcggtcgt
       61 aagagcagag acccttgtcg gtccgctcct taagcttggc tggcccttgc ccagcgcttg
      121 agcgctctgc gtcgtcggac agaacccgat ctcggattct aatctgaggc gtcggtcggc
      181 caagccatca tcgccgcctc ggctatgcct ttcaattcag cccgggtcgc gccgtcacga
      241 gcctggatgg ccatcccctg gaaaatgcca agaaaaaacc gactcagccg atcaatatcg
      301 gcggacgcag gcaactcgcc ttccgcgacg gcatcctcca gtcgcgaccg aaacatccgt
      361 aaactttcga gccgcgcgtt tctgacgtcc tcggcgatcg aggccggcca ttcgtcgctg
      421 actgcgccta gcatcgccat gcagccggcc ggcgtcgttt tggattcagg aaagttttca
      481 gccgccgcca gcagaaagct tcgcacccca gcgtgggcgg tgggggactc ggtgagcttg
      541 ttccagagcg gcgacccaca ggtgcagaga tatcgcgcaa ctgcttcccg atacagcgtc
      601 tctttgcaac caaaggccgc gtaaagactc ggcgacccga tgcccattgc gtcgcatagg
      661 tccttcatcg aggtcgtcgc aaatcctttg cgccaaaaca ggtgcatggc cgcgtccaac
      721 gccgcctcgc gatcaaactc tcgcggccga cctataacca cgatcgcctc cttatttctg
      781 taacgctgaa tacataattt gattgacacc cttgttcaat gagctagatc gttatatgtc
      841 gatcggcaca gaaaggaaac taaaaatgac gctcgcggga aaacgggccc tggtcacggg
      901 cgcaagcaga gggatcgggg cagcgatcgc caaggcgctg gctgccgaag gcgcggatgt
      961 ggcgatcacg tacgagaagt ccgccgatcg cgccgccgag gtcgtgcgcg ccatcaaagc
     1021 acagggccgg cgcgccgttg cgatccaggc cgacagcgca gatgccgccc ccgtgcgggc
     1081 ctcggtcgag aaaacggtcg ctgagcttgg cggtctcgac atcctggtta acaatgccgg
     1141 catccttcga gtctcagagc taaaggacat ttcgctcgac gacatcgacg cgattctgga
     1201 tgtcaacgtc cgtgcgccga ttgtcgccag caaggcggcc attccccatc tcggcaaagg
     1261 cggtcgtgtc atctccatcg gaagtttctt cgcagaccgc gtgccctccc ctgtgctcag
     1321 cgtttacgcg gcatcgaagt ccgcgctggt cggcttcact aaggggcttg cccgcgaagt
     1381 cggtccaaaa ggcatcacag tcaacctggt gcagccaggc tccatcgaca ccgacatgaa
     1441 cccggctgac ggcccgtttg cggagacgct tcggcaaatg actgcgttcg gccggtatgg
     1501 tacggtcgac gatatcgccg atgccgtggc gttccttgcg agcccaaagg cacaatacat
     1561 caccgggacg acgttgaccg tcgatggcgg cgcgatcgct taaattgcaa agcaccgggc
     1621 gcccccgaag cgcccggccg tggtcctgcg gcgaagaagc cgcgatgaag attgagagaa
     1681 atgccgtgga cgaaattagc agccgagcag gcattcaaaa gtcggccaaa cctacgtcac
     1741 ggcggacacg atatataata tggattgctg cagccgcgac gcttttgctc attttggctg
     1801 tgggatctag ccatttcttc ctgcacgacg gtcaggctgg cccgcaggat gctggcgccc
     1861 aatcactccc acaagtcatc gtcagcacac ccctcgaacg gcaaatcgac aaagagctga
     1921 cgttcctcgg acaattctcg gccgtcgatc gtgtcgaact tcgagcgcag gtcggtggta
     1981 tattgaccgg aatttatttc aaggatggcg ctatagttcg taaaggcgat ctgctcttca
     2041 ccatcgatac ggtgccttat gcaattaagc tcgcgcaggc ccaggc
//
LOCUS       DASWOG010000069         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_331395,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000069 DASWOG010000000
VERSION     DASWOG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331395"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1418)
                     /locus_tag="VGB93_01350"
     CDS             complement(192..1418)
                     /locus_tag="VGB93_01350"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0144807.1"
                     /translation="MNKIKVANPVVELDGDEMTRIIWHMIRDKLIHPYLDIHLDYYDL
                     SMENRDATDDQVTVEAANAIKKHGVGVKCATITPDEARVKEFNLKEMWKSPNGTIRNI
                     LGGVIFREPIICKNVPRLVPGWTAPIVVGRHAFGDQYRATDFKVPGKGRMTIRFEGDD
                     GQVIEKEVFKYPGAGVAMAMYNLDDSIRDFARASLNYGLLRKFPVYLSTKNTILKAYD
                     GRFKDIFQEVYENEFKADYMAARITYEHRLIDDMVASALKWSGGYVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLLTPDGQTVEAEAAHGTVTRHYREYQKGRETSTNSIASIFAW
                     TRALGHRAKLDDNEELARFAATLEKVCIDTVESGFMTKDLALLVGDSQKWLSTNGFLD
                     KISDNLRKVLEPAAAV"
     gene            1622..>2345
                     /locus_tag="VGB93_01355"
     CDS             1622..>2345
                     /locus_tag="VGB93_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0144808.1"
                     /translation="MTGAGTNHTIPSLTGGPAIILVRPQLAVNIGMCARAMANFGLSD
                     LRLVNPREGWPRTGALRKGAYAAAAGAVHLLESARLFPSLEEAVADLNGVFATTARER
                     GQVKPVLAADEAMIEAAARIPSGEKLGILFGPERTGLANEEIALADKIVSFPVNPAYA
                     SLNLAQAVLLIGYEWMKAAHGSAPRFQQAERSPGAPREMVLSFFEYLEGELERVGFFR
                     PLAKRPVMQNNLRNMFHRMGLTE"
BASE COUNT          470 a          689 c          688 g          498 t
ORIGIN      
        1 tttcattcat gaaaggctcg tcttccgacg gtcgataatc gacatcgatc gtttcattcg
       61 gcacgttcaa catctccgca cctcagacga aatcgctggc cttataacga gatgcgcaag
      121 gctagacaat aagttccttg cctccgacag atgttttcaa cgtcgttccg aaataaccgg
      181 agcaaagcgc atcacacagc cgccgccggc tcaagcactt tgcgcagatt atcgctgatc
      241 ttgtcgagga atccgttggt cgagagccat ttctggctgt cgccgacgag caaagcgaga
      301 tccttggtca taaaacccga ttcgaccgtg tcgatgcaga ccttttctaa agtggcggcg
      361 aaacgcgcta gttcttcatt gtcgtcgagc ttggcgcgat ggccgagcgc acgtgtccag
      421 gcaaaaatcg atgcaatcga attggtcgaa gtctcgcggc ccttctgata ttcgcgataa
      481 tgccgcgtga cggtgccatg cgcggcttcg gcttcgacgg tctgcccatc cggcgtcagc
      541 aacacggagg tcatcaggcc gagcgaccca aagccctggg cgaccgtgtc cgattgcacg
      601 tcgccatcgt aattcttgca ggcccaaaca tagccgcccg accatttgag cgccgaggcg
      661 accatgtcat cgatcagccg atgttcgtag gtgattcgcg cggccatata atccgccttg
      721 aactcgttct cgtagacttc ctggaagatg tccttgaagc gcccgtcgta ggctttgaga
      781 atcgtattct tggtcgagag atagacggga aatttgcgca gcaacccata gttcagggag
      841 gcgcgggcga agtcccggat cgaatcgtcg aggttataca tcgccatcgc gacgccggcg
      901 ccgggatatt tgaaaacctc tttctcgatg acctgcccgt catcgccctc gaacctgatt
      961 gtcatccggc cctttccggg cactttgaaa tccgtcgcgc gatattggtc gccgaaggca
     1021 tggcgaccga cgacgattgg cgccgtccag cccggtacga gacgcggcac gttcttgcaa
     1081 atgatcggct cacgaaaaat gacgccgccg aggatgttgc ggatcgtgcc gttgggcgat
     1141 ttccacattt ccttcaaatt gaattctttg acgcgtgcct cgtcgggggt aatcgtcgcg
     1201 catttgacgc caacgccgtg cttcttgatc gcgttggcgg cctcgaccgt gacctgatcg
     1261 tccgtggcgt cgcggttttc catcgacagg tcgtaatagt cgagatggat gtcgagatag
     1321 ggatggatca gcttgtcgcg gatcatgtgc cagatgatcc gggtcatttc gtcaccatcg
     1381 agttcgacga cgggatttgc gaccttgatc ttgttcatgg caaaaccttt ggcattacgg
     1441 agggagcgcg gcccctatag caccggcgag tgggagcgga aagcggtatg gcgatagacc
     1501 ttgcgccgcc ttgcaatggt gacataaaag gcgatcgcgg gacgtttgta gggcattcca
     1561 ataagcgctt ttcgacgatg aaaagtcgcc tccccgccgc aagatcgccc atagagaggc
     1621 gatgactggc gctggcacaa atcataccat tccgagcctc acgggcggcc ccgcgatcat
     1681 tctcgtgcgg ccgcagctcg cggtgaatat cggcatgtgc gcccgcgcga tggccaattt
     1741 cggccttagc gacctgcggc ttgtcaatcc gcgcgagggc tggccccgca ccggcgcgct
     1801 acgtaaaggc gcatacgcgg ccgcggcagg cgctgttcac ctgctcgaat cagccaggct
     1861 ttttccgagc ctcgaagaag ccgtcgccga cctcaacggc gtcttcgcga cgacggcgcg
     1921 cgaacgcggc caggtcaagc cggttctcgc cgcggatgaa gcgatgatcg aagcggcggc
     1981 gcgcattccg tccggcgaga agctcggcat tctcttcggc cccgagcgga cagggcttgc
     2041 caacgaggag atcgcgctgg ccgacaagat cgtcagcttt ccggtcaatc ctgcctatgc
     2101 atcgctcaat ctcgcccagg cggtcctgct gatcggctac gaatggatga aggctgcgca
     2161 cggcagcgcg ccgcggtttc agcaagcaga gcgctcgccg ggggcgccgc gcgaaatggt
     2221 gctctccttc tttgaatatc tggaaggcga actggagcgt gtcggctttt ttcgacctct
     2281 ggcgaaacgg ccggtgatgc agaacaatct gcgcaatatg ttccaccgga tgggcctcac
     2341 ggaac
//
LOCUS       DASWOG010000070        12483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_129943,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000070 DASWOG010000000
VERSION     DASWOG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129943"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /gene="recG"
                     /locus_tag="VGB93_01360"
     CDS             <1..1761
                     /gene="recG"
                     /locus_tag="VGB93_01360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0144809.1"
                     /translation="KSLPVGERRFISGKLELWEGHRQMVHPDRIVDAEGLATMPLVEP
                     VYGLTEGLAPRALAKIIGAALTRLPKLPDWLAHTRAKPPGLPSFSEALEILHHPAEPE
                     AIASIAPARLRLAFDELLAHQLALALTRANLRRAVGRGSRGDGSRTDAIRKNLPFSLT
                     AAQENALAEIRTDLAAPERMLRLLQGDDGSGKTIVALLAMTDVVEAGRQAALMAPTEV
                     LARQHFESLQKYAAAAGLHLALLTGRDTAAVRARTLAALAAGEIDIVIGTHALFQESV
                     GFRDLGLAVVDEQHRFGVHQRLALGAKGTGTDILVMTATPIPRTLVLACFGDMDVSTL
                     RDKPPGRQPIETRALPLERIGDVAMRLQGAVAEGARAYWICPLVAESETLDVAAVEER
                     YAALKDMFGGQIGLVHGKMKGVERDAAMAAFARGDIKVLVATTVVEVGVDVPEASIIV
                     IEHAERFGLAQLHQLRGRVGRGQAKSTCLLLYKSPLSETAKARIEIMRETEDGFRIAE
                     EDLRLRGGGDAIGTQQSGLPGFRLAQVDIHADLLKLAHAEARNILATNPKLDGQQGKA
                     LLVLLYLFEREAAVKLLQAG"
     gene            1767..3041
                     /locus_tag="VGB93_01365"
     CDS             1767..3041
                     /locus_tag="VGB93_01365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0144810.1"
                     /translation="MRDYAEAAGKLIAPFVEADLFAGGILVAQNGVPLLRQGFGIANR
                     EWNVPHSITGKFRLGSLTKQFTATAILQLMERGRLKLDDRIARHFPDTPAASAHISLF
                     HLLTHTSGIPSYTSLPHFFAQDARRDHTPRELIALTENMPLDFPPGSAFKYNNGGYVI
                     LGHVIERLTRQSYEAYVRENILDPLGLQNTGYEHVEAIIPERVPGYRLKNGKIENAGF
                     LAMSVPHAAGAIYSTLDDLLTWQRALVAAKPFSPTSAALMFKDHGHGYGFGWGIQNQF
                     RRRQFVHAGGINGFSVVVSFYPDGDLFIAVLANIQAAPVQKIARDLAALHFDIEETKP
                     SLVLDAALLADYVGTYRLPSRRVLRVTQEGARLFVETGDYARQELVATDDQTFAARLV
                     DWELRFAADGVEQAQSVLFRRDGVEMSGTRED"
     gene            complement(3097..4173)
                     /gene="ispH"
                     /locus_tag="VGB93_01370"
                     /gene_synonym="lytB"
     CDS             complement(3097..4173)
                     /gene="ispH"
                     /locus_tag="VGB93_01370"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0144811.1"
                     /translation="MSETLLSEPEVTSKPKLQILLCAPRGFCAGVVRAIDAVEQALRI
                     YGAPVYVRHEIVHNKYVVEGLKAKGAIFVEELDEIPDTRQPVIFSAHGVPKSIHADAE
                     SRKIFALDATCPLVTKVHREAEAHFRRGHQVILVGHAGHPEVVGTLGQLPKGSIILVQ
                     TPEDVENLSVADEGNLAYVTQTTLSIDDTRQMVDALTRRFPKIIGPYKEDICYATTNR
                     QEAVKRIAPVVDAMIVVGSSNSSNSQRLREVAERSGCKLSRLVLRADDVEWDLFKDVK
                     SVGITAGASAPEVLVEEIMDAFAERFELNVEIVSTADESVFFPLPRELRPSNGAGRRG
                     RVGGEATEDQNDDTIQVEEELREH"
     gene            complement(4319..4777)
                     /locus_tag="VGB93_01375"
     CDS             complement(4319..4777)
                     /locus_tag="VGB93_01375"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0144812.1"
                     /translation="MAQIPDRIDPMALALDEARAAGARGEVPVGAIILRDGEVLARAG
                     NRTLELKDPTAHAEILAIRAACEVLGSERLIGCDLYVTLEPCAMCAAAISFARLRRLY
                     WGAPDPKGGAVEHGPRFFAQPTCHHAPECYGNIRESEAAALLRGFFLEKR"
     gene            4835..6532
                     /locus_tag="VGB93_01380"
     CDS             4835..6532
                     /locus_tag="VGB93_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0144813.1"
                     /translation="MPHDHGRPQRPKRPPVRPQAPDAEKKGERVAKVMARVGLCSRRD
                     AETWIAEGRVSVNGAVLTSPAMNIGPGDKVEVDGKPLAQRQRTRLFMFNKPRGLVTTN
                     KDPEGRQTIFDYLAMHHPDAPRLVTVGRLDINTEGLLLLTNDGGLARVLELPSTGWLR
                     RYRVRANGETDQAQLDTLKDGVTLDGIHYAGIEATLDRVQGANAWMTMSLREGKNREI
                     KRVLEHLRLEVNRLIRLSFGPFQLGDLNDGGLEEVRGRVLRDQLGETLLRESGADLDA
                     PLTAEPEPQTEERRPPRKGPAGAERKMRTHRPLAEPEEDKARHRPLPGPRKHISAMRT
                     QEDASGPRQRIERATTEDRKGRTIAVERHVLAKPPKKKSRFAEKFYSPAKADGAARAE
                     RPEAQGRPQKPGRAERPERGFAKRPERPAGKHPQRSPAQRPERTATERPEWSAAKRAE
                     RPAGKRPERNGAERPERSFAKRPERPAGKRPERSPAQRPERTGAERPEWSAAKRAERP
                     TGKRPERNGAERPERSFAKRPERPAGKRPERPAGKRPARAPQKPGRPDDSRRPPRGR"
     gene            6533..7090
                     /gene="rsmD"
                     /locus_tag="VGB93_01385"
     CDS             6533..7090
                     /gene="rsmD"
                     /locus_tag="VGB93_01385"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0144814.1"
                     /translation="MRIVAGRLRGRTIAGPTDDSIRPTSDRLRETIFNILAHAYDDAV
                     ADAAVIDLFAGSGAMGFEALSRGARRALFVDDAANARALLRANVDTLGLGGETRIFRR
                     DARKLGDAPTGEQFSVAFLDPPYGQHLAEQALMALRDGRWLTSDALVVVEESLESDVE
                     MPSGYRLLETRGYGGTKILFARAAS"
     gene            complement(7096..7413)
                     /locus_tag="VGB93_01390"
     CDS             complement(7096..7413)
                     /locus_tag="VGB93_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0144815.1"
                     /translation="MALEDRALAFAALGDETRLTLMGKLAGGPPQSIARLAEGSQLTR
                     QAITKHLRVLENAGVARSVRVGRENLFELRPEPLKDLQSYLERVSAQWDAALTRLKSF
                     VEE"
     gene            complement(7403..7831)
                     /locus_tag="VGB93_01395"
     CDS             complement(7403..7831)
                     /locus_tag="VGB93_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0144816.1"
                     /translation="MSRVWQALTDYRQFATWFRVDLDGPFVAGKVTRGRNTTCGYENF
                     LMEFTVRKIEPQTFFAYAWHPYAILPDVDYSGETPTLVEFRLAPHAGGTLLSVRESGF
                     DAIPEYRRAEAFRMHESGWAAQLANIEKHVTGHGEATLGA"
     gene            complement(7953..9224)
                     /gene="purD"
                     /locus_tag="VGB93_01400"
     CDS             complement(7953..9224)
                     /gene="purD"
                     /locus_tag="VGB93_01400"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0144817.1"
                     /translation="MNVLLLGSGGREHALALQISKSPLLGRLFAAPGNPGIAGVATCV
                     ALDPTDTTAVIDFCRVMDVGFVVVGPEAPLVAGLVDELAEAGIKAFGPRRAAAQLEGS
                     KGFTKDLCTAFAIPTAAAAQFSDADTAKEFIRSRGAPIVVKADGLASGKGVVVAESIA
                     EAEAAVDQMLGGLFGTAGQTLVIEEFLDGEEISFFALCDGKNARAFASAQDHKRVGEG
                     DTGPNTGGMGAYSPAPVMDDTLSTRVMDEIINPALRGMAQMGTPFVGVLFAGLMLTND
                     GPKLIEFNVRFGDPETQAILPRLQGDLLALMLACAEGRLIRQSFSLSAQHALTVVLAA
                     NGYPGTPKKGTEIRGLDKAAALPGVVITQAGTKREGAHLLADGGRVLNVTGLGATLAE
                     AQARAYAAVDAIDWPGGFCRRDIGWRALTGK"
     gene            complement(9299..11095)
                     /gene="mutL"
                     /locus_tag="VGB93_01405"
     CDS             complement(9299..11095)
                     /gene="mutL"
                     /locus_tag="VGB93_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0144818.1"
                     /translation="MPVRRLDPVLVDRIAAGEVIERPAAAVKELVENALDAGATRIDV
                     AIEAGGRRLIRVIDDGQGMGAADLELAVERHATSKLPDGDLSQIGTLGFRGEALPSIG
                     SVATLDIFSRATDTRYGSHIRVDQGLKHKLAPAAQPQGTRVEVRELFAATPARLKFLK
                     TDRTESLAVADVVQRLAMAHPEVRFALSGPDISGFDYAACTADAAGLLQRLTQILGKD
                     FHANALRLDAEREGVRLWGFAGLPTWHRANSNGQYFFVNDRPVRDKLFAGAARGAYVD
                     YLPAGRYPALALFVTCDPREVDVNVHPAKAEVRFRDPGLIRGLVVGAVKQTLEGARHR
                     ATPSNGSTALDLLARHPNNNGFSGWRPQRPANWTPETSVAYPGVAEDQARFDHITPSA
                     DVRAAVPLHDDDDAPLGAARAQIHGTYIIAQTSDGLVVVDQHAAHERLVYEKMKAARL
                     AGGIERQVLLIPTVVELEEAAVRRLDAAAPMLADFGLVIEAFGPGAILLREQPSALKE
                     LDADRLLRDLADVLAEDESAALPLERRLDHVLATLACHNSVRAGRRLLPEEMNALLRE
                     MERTPGSGQCNHGRPTYVELKLADIERLFGRR"
     gene            complement(11118..12407)
                     /locus_tag="VGB93_01410"
     CDS             complement(11118..12407)
                     /locus_tag="VGB93_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0144819.1"
                     /translation="MTDSLKTELKPTSSHAAKVQQIVSPGGIKAWLVEDYTVPLVSLE
                     LAFLGGASQDPAALPGVATLLAGLLDEGAGSYDSEAFHRILEEQAIEISFSADRDLLV
                     GRMQTLARNKAKAFELLGLAVTRARLDIEPLERVKSQISAGLRRETNDPDHVASRTFR
                     AIAYAGHPYGAPVRGDLATIDSLSRQHIADFRAASFARDNLTVAVVGAIDAPTLGTYL
                     DDVFGSLPQKASLLPVPDVVFAGVGRREIVDIDLPQSTIRFGRQGIPRDDADFMPGIV
                     VNHVLGGGVFSARLFREVREKRGLAYSVYSQLAAQDHSAMLFGGTSTKNERAAESLEV
                     IQGEIRSLSDAGPTDEELDKAKKYLIGSYALRFDSSTKIAAHLVHLQAEGYGVEYLDA
                     RNKLIEAVTMDDAKRVAKRLFGDGSLLVAVAGRPVGL"
BASE COUNT         2395 a         3964 c         3926 g         2198 t
ORIGIN      
        1 aagagcctgc cggtagggga acggcgcttc atttccggca agctcgagct ttgggaggga
       61 caccgccaga tggtgcatcc cgaccgcatc gtcgatgccg aaggcctcgc gacgatgccg
      121 ctggtcgagc cggtctatgg cctgaccgaa ggtctcgccc cccgcgcctt ggcaaagatc
      181 atcggcgccg ctttgacgcg actaccgaaa ctgccggatt ggctcgcaca cacccgcgcc
      241 aaaccgccgg gtcttcccag tttcagcgag gcgctcgaaa tcttgcatca tccggccgag
      301 cccgaggcca tcgcctcgat agcgccggca cggctgcggc tcgcgttcga cgaattgctc
      361 gcgcatcaat tggcgctcgc cctgactcgc gccaatctgc gccgcgcagt cgggcgcggc
      421 agtcgaggcg atggctcgcg caccgacgcg attcgcaaaa acctgccctt ctcgctcaca
      481 gccgcgcagg agaatgccct cgcggaaatc cgcaccgatc tcgcggcgcc ggagcgcatg
      541 ttacggctgc tgcaaggcga tgacggctca ggcaagacga ttgtcgcgct gctagcgatg
      601 acagacgttg tcgaggccgg gcgccaggcg gcgctgatgg cgccaaccga agtgctcgcc
      661 cggcagcatt ttgaatcgct gcaaaaatat gctgccgccg cgggtttgca cctcgctttg
      721 ctcaccggcc gcgatacggc ggcggtccgc gcccgcacac tggcggcttt ggccgcgggc
      781 gagatcgaca tcgtcatcgg gacacatgcg ttgtttcagg aaagcgtcgg gttccgcgat
      841 ctcggcctcg cggtggttga cgagcagcat cgcttcggcg tgcatcaacg gttggcgctt
      901 ggtgccaaag gcaccggcac cgatattctg gtgatgacgg caacaccaat cccgcgcacg
      961 ctcgtgctcg cctgtttcgg cgacatggat gtttcgacgt tgcgcgacaa gccgccgggc
     1021 cgtcagccga tcgaaacgcg ggcgctgccg ctcgagcgca tcggcgatgt cgccatgcgc
     1081 ttgcaaggcg ccgtcgcgga gggtgcacgc gcctattgga tttgcccgct cgtcgcggaa
     1141 agcgagacgc tggacgtcgc tgctgtcgag gaacgctatg ccgcgctcaa ggatatgttc
     1201 ggcggccaga tcggcctcgt gcatggcaaa atgaaaggcg tcgagcgtga tgcggcgatg
     1261 gcggctttcg cgcgcggcga catcaaagtg cttgttgcga caacggtggt cgaggtcggc
     1321 gtcgatgtgc cggaagcctc gatcattgtc atcgaacatg ctgaacgttt tggcctcgcg
     1381 caattgcatc aattgcgcgg gcgggttggt cgtggccaag ctaaatcgac gtgccttttg
     1441 ctctataaat cgcctctcag cgaaaccgcc aaggcgcgca tcgaaatcat gcgcgagacg
     1501 gaagacggtt tccgcatcgc cgaggaagat ttgcgcttgc gcggcggcgg cgatgccatc
     1561 ggcacgcagc aatccggcct gcccggcttc cgactggcgc aagtggacat tcacgcggac
     1621 ctgctgaagc ttgcgcatgc cgaggcgcgc aatatcctag ccacaaatcc gaaactcgac
     1681 ggccaacaag gcaaggcgct ccttgtgctg ctgtacctat tcgagcgtga ggcggcggtg
     1741 aaattattgc aggcgggata gcgaatatgc gtgattacgc cgaagccgcc ggaaagctca
     1801 tcgcgccatt tgtggaggcc gatctttttg ccggcggcat ccttgtcgcg cagaacggcg
     1861 tgccgctgct gcggcagggc tttggcattg ccaatcgcga atggaatgtg ccgcacagca
     1921 tcaccggcaa atttcgcctc ggctcgttga ccaagcaatt caccgcaacc gcgatcctgc
     1981 aattgatgga gcgcggacgt ctcaagcttg acgataggat cgcgcggcat tttcctgata
     2041 cgcccgcggc gtcggcgcat atcagcctgt ttcatctgct gacgcatacc tcgggcattc
     2101 cgagctatac gtcgctgccg catttcttcg cacaggacgc gcggcgcgac cacaccccac
     2161 gtgaactcat cgcgctcacc gaaaatatgc cgctcgattt cccacctggc agcgcgttta
     2221 aatataacaa cggcggctac gtgatcctcg gccacgtcat cgagcgtctg acgaggcaga
     2281 gctacgaagc ttatgtgcgc gaaaatattc ttgacccgct cggcctgcaa aacaccggtt
     2341 acgaacatgt cgaggcgatc attcccgaac gcgtgccggg ctatcgcttg aagaatggca
     2401 agatcgagaa cgccggattt ctcgcgatga gcgtgccgca tgcggctggt gcaatctatt
     2461 cgacgctcga cgatctcctg acatggcaac gggcgctcgt cgcggcgaaa ccgtttagcc
     2521 ctacctcggc cgccttgatg tttaaggatc acgggcacgg ctatggcttt ggctggggga
     2581 ttcaaaacca gttcaggcgg cgacaattcg ttcatgccgg cggtatcaac gggttcagcg
     2641 tcgtcgtgag cttttatccg gacggcgatc tgttcatcgc cgtgctggcc aacattcagg
     2701 ccgcgccggt gcagaagatc gcccgcgatc ttgcggcctt gcatttcgac attgaggaaa
     2761 cgaaaccgtc gctcgtgctc gatgccgcgc tgctcgccga ttatgtcggg acgtatcggc
     2821 ttccatcccg ccgcgtgttg cgagtcacgc aggaaggcgc ccggcttttc gtcgagacgg
     2881 gggattacgc acggcaggaa ttagtcgcga ccgacgacca aactttcgcc gcgcggcttg
     2941 tcgattggga gttgcgcttt gcggccgatg gcgtcgaaca ggcgcaaagc gtcttgtttc
     3001 gccgcgatgg tgtggagatg agcggaacgc gcgaagacta gaggatgatc cggaaaagtg
     3061 gttgccggtc ttccgaaaag atcatgctca aaaaaatcaa tgctcgcgca attcctcttc
     3121 gacctgaatc gtatcgtcgt tctggtcttc agtcgcctcg ccacccacgc ggccgcgacg
     3181 gccagcgccg ttcgacggcc gcagttcgcg cggcagcggg aagaagacgc tttcatccgc
     3241 cgtcgagacg atctcgacat tgagttcgaa gcgttccgcg aaagcatcca tgatctcttc
     3301 gacgagcact tccggcgccg aagcgcccgc cgtaatgccg acggacttca cgtccttgaa
     3361 gagatcccat tcgacatcgt cggcgcgcaa gacgaggcgt gacagcttac agccggagcg
     3421 ctcggcgacc tcgcgcaggc gctgcgaatt cgacgagttc gacgatccca cgacgatcat
     3481 cgcatcgacg acgggcgcaa tgcgcttcac cgcctcctgg cgattggtcg tcgcgtagca
     3541 gatgtcttcc ttgtaggggc cgatgatctt gggaaagcgg cgcgtcagcg catcgaccat
     3601 ctggcgcgtg tcgtcgatcg acagcgtcgt ctgcgtgaca taggcgagat tgccctcatc
     3661 ggcgacgctc aggttttcga catcctcggg tgtctgcacc aggatgatcg agcccttcgg
     3721 caattgaccg agcgtgccga caacttccgg atgaccggcg tggccgacga gaatcacctg
     3781 atggccgcgg cggaagtgcg cctccgcctc gcggtgaacc ttggtcacga gtgggcaggt
     3841 cgcgtcgagc gcgaaaatct tgcgagattc cgcatcggcg tggatcgact tcggcacacc
     3901 atgcgccgag aaaatgaccg gttgccgcgt atcggggatt tcgtcgagtt cctcgacgaa
     3961 gatcgcgccc ttggccttca gcccttcgac gacgtatttg ttatggacga tctcgtggcg
     4021 gacatagact ggcgcgccgt agatgcgcaa agcctgctcg accgcatcga tggcgcggac
     4081 gacgccggcg cagaagccac gcggggcgca gagaagaatc tgcagcttgg gcttgctcgt
     4141 cacctcaggc tcggagagca acgtctctga catcgatgat ttccatcaag tgattgggat
     4201 cgttaatcaa tcctgccacc cgcaaggcgg acggcggccg catcgcagca ttcatcttgg
     4261 ttgtgggctg ggtgtcgcgc cctgtcaaga gataggcgcc gcggcggttt ttcccccctc
     4321 agcgcttttc caggaaaaag ccgcgcagga gggccgccgc ctcgctttcg cgaatgttcc
     4381 catagcattc cggcgcatga tggcatgtcg gctgggcaaa aaaacgcggc ccatgctcca
     4441 ccgccccgcc cttggggtcc ggcgcgcccc aatagaggcg gcgcaggcgc gcgaacgaga
     4501 tcgccgcggc gcacatggcg caaggctcaa gcgtcacata gaggtcgcag ccaatcagcc
     4561 gctcggagcc cagcacctcg caggcggcgc ggatcgccag aatttcagcg tgggcggtcg
     4621 ggtccttcaa ttccagcgtc cggttgccgg cgcgggccag aacctcgcca tcgcgcagga
     4681 tgattgcgcc gactggcact tcgccgcgcg cgccagccgc ccgcgcctca tcgagtgcga
     4741 gggccattgg atcgatgcgg tctggtattt gagccatgaa aaccttttct atccgcttgg
     4801 ctgttccttc cgcttggttc tgctatcaaa cgccatgccc catgaccatg gccgtcccca
     4861 aagaccgaaa cgtccgcccg tccgcccgca agcgcccgat gctgaaaaga agggcgagcg
     4921 cgtcgccaag gtgatggcgc gcgtcgggct ttgctcgcgc cgcgacgccg agacgtggat
     4981 cgccgaaggc cgcgtctccg tcaacggagc cgtgttgact tcgcctgcca tgaacatcgg
     5041 cccgggcgat aaggtcgaag ttgacggcaa gccattggcg caacgccagc ggacgcggct
     5101 tttcatgttc aacaagccgc gcggcctcgt cacgacgaac aaagatccgg aaggccggca
     5161 aacgatcttc gattatctcg ccatgcatca ccccgacgcg ccgcggctcg tcaccgtcgg
     5221 ccggctcgat atcaacaccg aaggcctgct gctgctcacc aacgacggcg gcctggcgcg
     5281 cgttctcgaa ctgccttcga caggctggct gcgccgctat cgtgtccgcg ccaacggcga
     5341 gacggatcag gcgcaactcg acacgctgaa agacggcgtg acgctcgatg gcattcatta
     5401 tgccgggatc gaggccacgc tcgatcgcgt gcagggcgcc aacgcctgga tgaccatgag
     5461 cctgcgcgag ggcaaaaatc gcgagatcaa gcgcgtattg gaacatctcc gccttgaagt
     5521 gaaccggctc atccggctgt ctttcgggcc gttccagctc ggagatctga atgacggcgg
     5581 attggaagag gtccgtggcc gtgtgttgcg cgatcaactc ggcgaaaccc tgctgcgcga
     5641 atcgggcgcc gatctcgacg cgccattgac cgccgaaccc gaaccgcaaa ccgaggaacg
     5701 acggccgccg cgaaaggggc ctgccggcgc agagcgcaaa atgcggacgc accggccctt
     5761 ggcggagccc gaagaggaca aggcgcgcca tcgtcccctc cccggcccgc gcaaacatat
     5821 ctccgcgatg cgcacgcaag aggatgcgag cggtccgcgt cagcggatcg aacgtgcgac
     5881 gacggaagat cgcaagggcc gcacgattgc cgtcgaacgg catgttcttg ccaaaccacc
     5941 gaagaagaag tcgcgcttcg ccgagaagtt ttattcgcca gcaaaggccg acggcgcggc
     6001 gagagccgag cggccagagg cgcagggccg tccgcaaaaa ccgggacgcg ccgagcggcc
     6061 cgaaaggggt ttcgcgaaac gcccggaacg gcctgcggga aagcacccgc agcgcagccc
     6121 ggcgcagcgt cctgagagga ccgccacgga acggccagag tggtctgccg caaaacgcgc
     6181 ggagcgtccc gccgggaagc gcccggaacg aaatggagcg gagcggcccg aaaggagctt
     6241 cgcaaaacgt ccggaacggc ctgcgggaaa gcggccggag cgcagccctg cgcagcgccc
     6301 cgagaggacc ggagcggaac ggccagaatg gtctgccgca aaacgcgcgg agcgtcccac
     6361 cgggaagcgc ccggagcgga atggagcgga gcggcccgaa aggagcttcg caaaacgacc
     6421 ggaacggcct gcgggaaagc gcccggaacg gccggccgga aagcggcctg cgcgcgcgcc
     6481 gcaaaaaccc ggacggccgg atgactcccg ccggccgcca cgcgggcgat agatgcggat
     6541 cgttgccgga cgtttgcgtg gacgcacgat cgcgggcccg acggacgatt cgatccgccc
     6601 gacatcggat cgtctccgcg aaacgatttt caacattctc gcgcacgctt atgacgatgc
     6661 ggtcgcggac gccgcggtca tcgatctctt tgccggctcc ggcgcgatgg gtttcgaagc
     6721 cttgtcgcgc ggcgcgcggc gcgcactctt cgtcgatgat gccgcaaatg cgcgcgcgct
     6781 gcttcgcgcc aacgtcgata cgctcggcct cggcggcgaa acgcgcattt tccgccgcga
     6841 tgcgcgcaag ctcggcgatg cgccaacggg cgagcaattc agcgtcgcct ttctcgatcc
     6901 accctacggg cagcatctgg cggagcaagc gctgatggcg ctacgcgacg gtcgctggct
     6961 cacgtccgac gctttggtcg tcgtcgaaga aagcctcgaa tcggacgttg aaatgccgag
     7021 cggttatcgg ctgctcgaaa cgcgcggcta cggcggcacg aaaattctct tcgcgcgggc
     7081 ggcaagctga caggattatt cctcgacgaa ggacttcagc cgcgtcaaag ccgcgtccca
     7141 ttgcgcggaa acgcgctcca gataggattg caaatccttc agcggttcgg gccgcagctc
     7201 gaacagattt tcgcggccga cgcgcacgct gcgcgcgact ccggcgttct ccagaacgcg
     7261 cagatgtttg gtaattgcct gacgcgttag ttgcgaacct tcggcaagcc gcgcgatcga
     7321 ttgcggcggc ccgccggcaa gtttgcccat caacgtcagg cgtgtttcat cgcctagggc
     7381 cgcaaaggcc agagcgcgat cttcaagcgc caagtgtcgc ttcgccgtga ccggtgacat
     7441 gtttctcgat attggccaat tgcgccgccc agccgctttc gtgcatccgg aaggcttccg
     7501 cccggcgata ttcaggaatg gcatcaaatc ccgattcgcg caccgagaga agcgtcccac
     7561 ccgcgtgggg ggcgagccgg aactcgacca gcgtcggtgt ttcgccgctg taatcgacat
     7621 caggcaggat tgcgtacgga tgccatgcgt aggcgaagaa cgtttgcggc tcgattttgc
     7681 gcaccgtgaa ttccataagg aaattctcat agccgcacgt cgtgttgcgg ccgcgcgtga
     7741 ccttcccagc gacaaacggc ccatcgagat cgacacggaa ccaggttgcg aactggcgat
     7801 aatcggtcag cgcctgccag acgcgcgaca ctggcgcatt ctattcgatg tgcttttcaa
     7861 tgaagtcaga catgggcaac ctcatggttg ccttttaata tgccaaagtg cggcgatacg
     7921 caaccatttt gttgcatatt ttttgaatct gactatttgc ccgtgagcgc ccgccaaccg
     7981 atgtctcggc ggcagaagcc gccgggccag tcgatcgcat cgacggctgc gtaggcccgg
     8041 gcctgcgcct cggcaagcgt cgcgccgaga ccggtgacgt tcagcacccg gccgccatcg
     8101 gccagcaggt gggcgccctc ccgtttcgtc cctgcctgcg tgatgacgac gcccggcagc
     8161 gccgcggctt tgtcgaggcc acggatctcc gtgcccttct tcggcgtgcc gggataaccg
     8221 ttggcggcga ggaccactgt cagcgcatgt tgcgccgaca aggaaaagct ctgccggatg
     8281 agccgcccct cagcgcaggc gagcatcagc gcgagaagat cgccttggag ccgcggcagg
     8341 atcgcctgcg tttcgggatc gccgaagcgg acgttgaact cgatgagctt cggtccgtca
     8401 ttggtcagca tcaggccggc gaaaagcacg ccgacgaagg gcgtccccat ctgcgccatg
     8461 ccgcgcaagg ccgggttgat gatttcgtcc atcacccgcg tcgaaagcgt atcatccatc
     8521 acgggcgccg gcgaataggc gcccatgccg ccggtgttcg gccccgtgtc gccttcaccg
     8581 acgcgcttgt gatcttgcgc cgaggcgaag gcgcgcgcgt tcttgccgtc gcagagcgcg
     8641 aaaaacgaga tttcctcgcc gtcgagaaac tcctcgatga ccaacgtttg tccggccgtg
     8701 ccgaacaggc cgcccagcat ctgatcgacc gccgcttcgg cctcggcgat gctctcggca
     8761 acgacgacgc ctttgcccga tgctaggccg tcggccttga cgacgatcgg agcaccgcgg
     8821 ctgcggataa attctttcgc tgtatcggca tccgaaaatt gggcggcggc ggcggtcgga
     8881 atcgcaaagg ccgtgcaaag atccttggtg aaacccttcg agccctcgag ctgcgcggcg
     8941 gcgcggcgcg gcccgaatgc tttgatgccc gcctccgcca attcatcgac aaggccggcg
     9001 accagcggcg cctcgggacc gacgacgacg aagccgacgt ccatcacccg gcaaaaatcg
     9061 ataacggctg tcgtatccgt ggggtcgagc gcaacgcagg tcgcgacccc cgcaatgccc
     9121 ggattgccgg gcgcggcaaa gagccggccc agaagcgggc ttttcgagat ctgaagggcg
     9181 agcgcatgtt cgcggccgcc ggagccgagg agaaggacgt tcacagccga aataggtttg
     9241 aatgaccgat gtagcgagat atccgtaaac ggatattttt atgcgcgcta ccgcgtcttt
     9301 accgccgccc gaagagccgt tcgatgtcgg cgagcttcag ttcgacataa gtcgggcgcc
     9361 cgtggttgca ttgacctgag cccggcgtgc gctccatttc tcgcaggagg gcattcattt
     9421 cctcgggcag cagccgccgg cccgcgcgca ccgaattgtg gcaagcaagc gtcgccagca
     9481 catgatcgag acggcgctcc agcggcaagg cggcactctc gtcttccgcc aggacgtcgg
     9541 ccagatcgcg caacagcctg tcggcatcaa gctctttgag cgcgcttggc tgttcacgca
     9601 gcagaatggc accggggccg aaggcttcga tcacgaggcc gaaatccgcg agcattggcg
     9661 ccgccgcgtc gaggcggcgg acagcagcct cttccaattc aacgaccgtc ggaatgagca
     9721 agacctgccg ttcaatgcca cccgccagac gcgcggcttt catcttttca tagacaagac
     9781 gttcatgcgc ggcgtgctga tcgacaacga cgagaccgtc gcttgtctgc gcgatgatgt
     9841 aagtgccgtg gatttgcgcc cgcgccgcgc caagcggcgc atcgtcatcg tcgtgcaaag
     9901 gcactgcggc gcgcacatcg gcgctcggcg taatatgatc gaaacgcgcc tgatcttccg
     9961 caacaccagg atacgcaacg gatgtctccg gcgtccagtt cgctggccgc tgcggccgcc
    10021 aaccggaaaa cccattattg tttgggtggc gcgccaaaag atcgagggca gtgctgccgt
    10081 tgcttggtgt cgcgcgatgg cgcgcgccct cgagcgtttg cttcaccgca ccgacgacaa
    10141 gaccgcggat aaggcccgga tcgcgaaacc gcacttcggc cttggccgga tgcacattga
    10201 catcgacctc gcgcggatcg caggtcacaa acaacgccag cgccggatag cgccccgccg
    10261 ggagataatc cacataggcg ccgcgtgccg cgccggcgaa cagcttgtcg cgcacgggcc
    10321 ggtcattaac gaaaaaatat tgcccgttgc tgttggcgcg atgccaggtc ggcaggccgg
    10381 caaagcccca gagccgcacg ccttcgcgct cggcatcgag ccgcagcgca ttggcatgaa
    10441 aatccttgcc gaggatttgt gtgagccgct gcaacagccc cgcagcatcg gccgtgcaag
    10501 ccgcgtaatc aaagccagaa atatctggcc ccgaaagtgc aaagcgcacc tcgggatgcg
    10561 ccatcgcgag acgctggacg acatccgcga cggccagcga ttcggtgcgg tcggtcttga
    10621 gaaatttgag tctggccggc gtcgcggcga acaattcgcg cacttcgacg cgtgtgcctt
    10681 gcggctgcgc ggcgggcgcg agcttgtgtt tcagcccctg atcaacacgg atatggctgc
    10741 cgtagcgagt atccgttgcg cgcgagaaaa tatcgagtgt cgcgactgag ccgatcgacg
    10801 gcagtgcctc gccgcggaag cccaatgtgc cgatctgcga aagatcgcca tccggcaatt
    10861 tcgaggtggc atgccgctcg acggcaagtt cgagatctgc ggcgcccatg ccctggccat
    10921 cgtcgatgac acggatgaga cggcggccgc cggcctcaat cgcaacatcg atgcgggttg
    10981 cgccggcatc gagcgcgttc tcaacaagct ctttcaccgc tgcagccgga cgttcgatca
    11041 cttcgccagc ggcgatgcga tcgacaagga caggatcgag acggcggacg ggcatgtcga
    11101 tccccgaact gaggtcctta caatcctacc ggccgcccgg cgaccgcgac gagcagcgac
    11161 ccgtcgccaa agaggcgctt cgcgacgcgc ttggcgtcgt ccatcgtcac ggcttcgatc
    11221 agcttgttgc gcgcatcgag atattcgacg ccgtagcctt cagcctgcag atgcacgaga
    11281 tgcgccgcga ttttggtcga ggaatcgaac cgcagcgcat aggaaccgat gagatatttc
    11341 ttcgccttgt cgagttcttc gtcggtcggc ccagcgtcgg aaaggctgcg aatttcaccc
    11401 tggatcactt cgagcgattc agcggcgcgc tcgttcttgg ttgatgtgcc gccgaagagc
    11461 atcgcgctat ggtcctgcgc cgcaagctgc gaatagactg aataggcgag gccacgcttt
    11521 tcgcgcactt cgcgaaacag ccgcgccgaa aagacaccgc cgccgagcac gtgattgacc
    11581 acgatgccgg gcataaaatc agcgtcatcg cgcggaatgc cctgccggcc gaagcgaatc
    11641 gttgattgcg gcagatcgat atcgacaatc tcgcgccgac ccacaccggc aaacacgaca
    11701 tcgggcaccg gcagcagtga cgccttctgc ggcagtgagc cgaaaacatc gtcgagatag
    11761 gtgccaagcg tgggggcgtc gatcgcgcca acgaccgcga ccgtcagatt gtcgcgcgcg
    11821 aaactcgcgg cacggaaatc cgcgatgtgc tgccgcgaca gcgaatcgat cgtcgcaaga
    11881 tcgccgcgca ccggcgcgcc ataaggatgg cccgcataag cgatggcacg aaaagtacgg
    11941 ctcgcaacgt gatccggatc gttggtctca cgccggaggc cggccgagat ctggcttttc
    12001 actcgctcca agggttcgat gtcgagccgt gcccgcgtga cggcgaggcc gagcagttca
    12061 aatgccttgg ccttgttacg cgccagggtc tgcatccgac cgacaagaag atcgcggtcg
    12121 gccgagaaac tgatctcgat cgcctgctct tcgaggatgc ggtggaatgc ttccgaatcg
    12181 taagatcccg cgccttcgtc gagcaggccc gcaagcaggg tcgccacgcc cggcagagcc
    12241 gccgggtctt gcgaggcgcc gccgagaaag gcgagttcga gcgacaccag cggcacggtg
    12301 tagtcttcaa cgagccaggc tttgatgccg cccggcgaga cgatctgctg gactttcgcc
    12361 gcgtgtgaac tcgtcggctt caattcggtc ttgagactgt cggtcatttt gcatcccgtt
    12421 gggcgtgatc gccggaaacc cagttccgcg gcggtcgcaa catttgaaaa ttaagccgca
    12481 cgt
//
LOCUS       DASWOG010000071         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_276503,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000071 DASWOG010000000
VERSION     DASWOG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276503"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(474..1325)
                     /gene="nadC"
                     /locus_tag="VGB93_01415"
     CDS             complement(474..1325)
                     /gene="nadC"
                     /locus_tag="VGB93_01415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0144820.1"
                     /translation="MNLAPLSRLVLEPLVRAALIEDIGLAGDITTNAIVPPEAETRAL
                     FVARKPGVVAGLDLAKLSFELVDPSLTFSVAKPDGTHVAVGETIATVSGRAASILIGE
                     RVALNFLGHLSGIATATAKFVKAVEGHKAKIVCTRKTTPGLRAIEKYAVRAGGGQNHR
                     FGLYDEVLIKDNHIAIAGGVTAALRRAKKHVGHMVKIEVEVDSIEQLREALLEKPDVI
                     LLDNMNPAQLAEAVKIVDGRAITEASGGVSLETAAAVAASGVDLIAIGWLTHSAQTLD
                     IGLDFPD"
     gene            complement(1325..2836)
                     /locus_tag="VGB93_01420"
     CDS             complement(1325..2836)
                     /locus_tag="VGB93_01420"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY0144821.1"
                     /translation="MDIRDLEGRVLIVGGGIAGLMTALKLAPEPCVVLSRGPIGEDTS
                     SILAQGGVAACIGFDDSVALQVADTLAAGDGLCDAPVVEKIVAGGPAAIEDLVRLGVP
                     FDRDANGNLRRGLEAAHNRHRIIHAGGDKTGREITLALAAAVHAAPSVTILEGFAARR
                     LNVEDDAVSGLVAEGAGGTVFFRTNRIVIATGGVSGLFAHGTNPVGSWGQGLAIAARA
                     GATLSDMEFVQFHPTALDGTGPQVSLISEAVRGEGAILINENGERFMAGVAGAELAPR
                     DIVARAVWAQLRAGHRTFLDARNVKDLSSHFPGVTQHCRTAGVDPVRDPIPVRPAAHY
                     HMGGIKVNLEGRTNIEGLWAVGEAAATGLHGANRLASNSLLEALVCARFVADSLNAAP
                     RRTLRATPAAQPMRPNDLQAVRAIVSRAAGVLRDGADLRRAASELYPLAATNDAALVA
                     LMIVAAALRREESRGAHYRLDFTQKDAMPKWPREVDSTQAFAAARELSLADVA"
     gene            complement(2847..>3333)
                     /gene="nadA"
                     /locus_tag="VGB93_01425"
     CDS             complement(2847..>3333)
                     /gene="nadA"
                     /locus_tag="VGB93_01425"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0144822.1"
                     /translation="GVPRVIMIPDEYLAKNIAAETGVEIITWKGHCEVHELFGTEEVA
                     QLREDYPGVVILAHPECAPDVVQASDFSGSTAAIANYVEKKRPERVALLTECSMSDNI
                     SVHYPELEFIRPCNLCPHMKRITLSNIRHSLETMQHEVEIDPSTAVRARLSVERMLAV
                     S"
BASE COUNT          648 a         1043 c         1039 g          603 t
ORIGIN      
        1 gacatcgatt gtcggcaggg cgtcagtgac agccgcgcca ctctgagcag cagcggaact
       61 ttgccaggcc acgagaccga acagaagggc aaacgccttt aagccattgc gatccagatg
      121 aagagaatgg ccgcgatttg cgcgcgcagc accggaccgt gcccgcgatg ggcacagcat
      181 taaagatgac atcgaaatat cctcaaacca gctcgcagcc gccgaaacgg ctgcttcaat
      241 acggagctgg gcgttgcgcc ccgatgcgat tggtggctct taagactttc cgatccgaac
      301 attggcgggc gccaacacag atcggtcccg atcgctaaga tggttcttta tttgccatgg
      361 acagcaaccg caaatgactt ataagtcatt gttataacta gaattattca aatagtatta
      421 tacttcaggt atacttgcgt gccgcgcgat tggacggtaa tcgaagcgtt tggtcagtcg
      481 ggaaaatcga gcccgatatc gagcgtctgg gcgctatgcg tgagccagcc gatggcgatg
      541 agatcgacgc cgctcgccgc gacggcggcc gcggtctcca gcgagacacc gcctgacgct
      601 tcagtgatgg cgcggccatc gacgatcttt acggcctccg ccaattgcgc ggggttcatg
      661 ttgtcgagca ggatgacatc cggcttctcg agcagcgcct cgcgcaattg ttcgattgaa
      721 tcgacctcga cctcgatttt gaccatgtgg ccgacatgct tcttggcccg ccgcagcgct
      781 gccgtcacgc cgccggcgat ggcgatgtgg ttgtccttga tcagaacctc gtcgtagagg
      841 ccgaagcgat ggttctggcc gccgccggcg cgcacggcat atttctcgat cgcgcgcagg
      901 cccggcgtcg ttttccgcgt gcagacaatc ttcgccttgt ggccttcgac agctttgacg
      961 aatttagcag ttgccgtggc gatgccgctc agatgaccga gaaaattcag cgccacgcgc
     1021 tcgccgatga gaatagaggc cgccctgcca ctgacggtcg caatcgtctc gccgaccgcg
     1081 acatgcgtac cgtcgggctt cgcgacggaa aaggtcagcg acggatcaac aagttcgaag
     1141 ctgagtttcg ccagatcaag gcccgcgacg acgcccggct tgcgcgcgac gaacagcgcc
     1201 cgcgtttcgg cctcgggcgg cacgatcgca ttggtcgtga tgtcgccggc aaggccgata
     1261 tcctcgatga gcgctgcgcg caccaaaggt tcgagaacaa gccgcgacag gggcgccaga
     1321 ttcatcaggc gacgtcggcg agcgaaagtt cgcgcgcggc ggcgaaggcc tgcgtgctat
     1381 cgacctcgcg cggccatttc ggcatggcgt ccttctgtgt aaaatcgagt cggtaatgcg
     1441 cgccgcggct ttcctcgcgc cgcaaggcgg cggccacgat cattagcgcg acaagcgctg
     1501 catcattggt cgcggcaagc gggtaaagtt cgcttgcggc ccggcgcagg tccgcgccgt
     1561 cgcgcaacac gcctgccgcg cgcgagacaa tggctcggac ggcttgcaga tcgttcgggc
     1621 gcattggctg ggcagcgggc gtagcgcgca gcgtccggcg cggcgcggcg ttgagactat
     1681 cggcaacgaa gcgcgcacag accaaagctt cgagcagcga attgctggct agccggttgg
     1741 cgccatgaag accggtcgcg gcggcctcgc cgaccgccca cagcccttca atattggtgc
     1801 ggccttcgag gttcaccttg atgccgccca tgtgataatg cgccgccgga cgcaccggga
     1861 tcggatcgcg caccggatcg acgccggcgg tgcggcaatg ctgcgtcacg ccggggaagt
     1921 gcgatgataa atccttgaca ttccgggcgt cgagaaaggt gcggtgtccg gcgcgcaatt
     1981 gcgcccagac ggcgcgggcg acgatatcgc ggggcgcgag ttccgccccc gcgacaccgg
     2041 ccatgaagcg ctcgccattt tcgttgatga ggatcgcgcc ctcgccccgc accgcttcgc
     2101 tgatgaggct gacttgcggt cctgtaccat cgagcgcggt cggatggaat tgcacgaatt
     2161 ccatatcgct caatgtagca cctgcgcgcg ccgcaatcgc aagaccctgg ccccaggagc
     2221 cgacggggtt tgtgccgtgc gcgaaaagcc cgctgacgcc gccggtcgcg atgacgatcc
     2281 gattggtgcg aaagaaaacg gtgccacccg caccttcggc cacgagtccc gacaccgcat
     2341 catcttcaac attcaaacgg cgggcggcaa aaccttcgag gatcgtgacg gagggcgccg
     2401 catggacggc agcggccaaa gccagtgtga tctcacggcc ggtcttgtcg ccgccggcgt
     2461 ggatgatgcg atggcggttg tgcgcggcct cgagtccgcg ccgtaaattg ccgtttgcat
     2521 cgcgatcgaa cggcacgcca agacgcacga ggtcttcaat cgcagccggg ccgccagcga
     2581 caatcttctc gacaacaggc gcgtcgcaaa gaccatcgcc tgccgcgagc gtgtcggcga
     2641 cttgcagcgc gacgctgtcg tcgaagccga tgcaggcggc gacgccgcct tgcgccagaa
     2701 tgctcgacgt gtcttcgccg attgggccgc ggctcagcac aacacaaggc tctggcgcaa
     2761 gcttcaaagc cgtcatcagt ccggcgatac cgccgccgac gatcagcacg cgcccttcaa
     2821 gatcgcgaat gtccatcagc tttgccttaa gagaccgcca acatgcgttc gaccgaaagc
     2881 cgcgcccgca cggcggtcga aggatcaatc tccacctcgt gctgcattgt ctccagcgaa
     2941 tgccggatgt tcgacagcgt gatccgcttc atgtgcgggc agagattgca gggccggatg
     3001 aattcgagtt ccggataatg caccgagata ttgtcgctca tcgaacattc ggtcagcagc
     3061 gcgacccgct ccggccgctt cttctcgacg taattggcaa tcgctgcggt ggaaccggaa
     3121 aagtccgaag cctggacgac atcgggggca cattcgggat gcgcgagaat gacgacgccg
     3181 ggataatcct cacgcaattg cgcgacttcc tcggtgccga aaagctcgtg cacttcgcaa
     3241 tggcccttcc aggtgatgat ctcgacgccc gtctcggcgg cgatgttctt cgccagatat
     3301 tcgtccggga tcatgatcac gcgcggcacg ccg
//
LOCUS       DASWOG010000072         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_74952,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000072 DASWOG010000000
VERSION     DASWOG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74952"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1028
                     /locus_tag="VGB93_01430"
     CDS             90..1028
                     /locus_tag="VGB93_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0144823.1"
                     /translation="MDRFEAMSIFLRVVEKGSFSAASRDLRMPLATVSRKVGDLEVHL
                     GSKLLIRSTRKLALTEAGAVYLASARRILEEVSEAEQLAAGEFHMPRGELILTAPVLF
                     GRLHILPVVTDFLAAYPEINVRLTLSDRNLHLIDDHIDMAVRIGRLPDTSMVATRIGT
                     VRPVVCASPGLLARYGTPKSPADLARWPCVNFDFFSPAPVWPFRQQGAKAAMEMPIHP
                     RLSVSTAEAAVAAATEGVGATRVLHYQCAEALNAGALRIMLADFELDPLPIHLLHAGR
                     AALPSKTRVFLEFATARLRQRLKHLPPAATNDAIED"
     gene            complement(1043..1507)
                     /locus_tag="VGB93_01435"
     CDS             complement(1043..1507)
                     /locus_tag="VGB93_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144824.1"
                     /translation="MRPYQFSETRQRLGPRRNSMEEQMDRIVEFKLQILRAIAVLFDA
                     FERPTIGPDEVLGCCLQHNAREFEQLSSRSNETIYWLYKRGIVKGILEDRAAVSYLAH
                     ARLAEWVRAILTKELSKETGESLADLVSGRARYGGPDEMIAVSNLLFHRLSR"
     gene            2196..2432
                     /locus_tag="VGB93_01440"
     CDS             2196..2432
                     /locus_tag="VGB93_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144825.1"
                     /translation="MNWYGNETTNTWDSHITKALTSAYDDALARAEKLGILGEKTGHA
                     ASDIIARHIIEGAKRGKYDPQLLAAGALERLQRG"
     gene            2604..2840
                     /locus_tag="VGB93_01445"
     CDS             2604..2840
                     /locus_tag="VGB93_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144826.1"
                     /translation="MKKLIAAALFASVVVVASPAFAITFSLIQDLDTGNCAAVVTSPN
                     GWPGMKAVSETTYSSQEEADKALDSVKACKSFVR"
     gene            complement(3677..3766)
                     /locus_tag="VGB93_01450"
     tRNA            complement(3677..3766)
                     /locus_tag="VGB93_01450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3730..3732),aa:Ser,seq:cga)
     gene            3908..4129
                     /locus_tag="VGB93_01455"
     CDS             3908..4129
                     /locus_tag="VGB93_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144827.1"
                     /translation="MNTSDPTERPGSDWPKSLALGFVFLWFFVGGIAHFAFTAREMEI
                     VPPPFPAHRALVLMLLFLIYWSTRPPEAA"
     gene            complement(4134..>4395)
                     /locus_tag="VGB93_01460"
     CDS             complement(4134..>4395)
                     /locus_tag="VGB93_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEY0144828.1"
                     /translation="MDRRYLGLIASSFGGGPVGIETLAAALSEPRDAIEDIIEPYLIQ
                     QGFLQRTPRGRVLTPRAFEHLGLDAPARPQQFGLFMQGDEPE"
BASE COUNT          948 a         1239 c         1246 g          962 t
ORIGIN      
        1 cccctcggcc cccacaacca acgctaggat gttccaataa aagcgataat cagctaagat
       61 atagaatgac attctcatga aatgagataa tggaccgctt cgaagccatg tcgatctttc
      121 tgcgcgtggt ggagaaaggc agtttctccg ccgccagccg cgatttgcgc atgccgcttg
      181 cgacggtcag ccggaaggta ggcgaccttg aggtgcatct cggctcgaag ctgctgattc
      241 gctcgactcg aaaactggca ttgaccgaag ccggcgcggt ctatctggct tcggctaggc
      301 gcattctcga agaggtcagt gaagccgagc aattggccgc gggcgaattt catatgccgc
      361 gcggcgaatt gattttgacg gcgccggtcc tattcggccg cctgcacatc ttgccggtcg
      421 tgaccgactt tctcgccgct tatcccgaga tcaacgtgcg gctgacgctc tccgatcgca
      481 atctgcatct gatcgacgat cacatcgata tggcggtgcg gatcggccgg cttcccgaca
      541 ccagcatggt cgcgacgcgc attggcaccg tgcgcccggt cgtctgtgcc agtccgggtc
      601 tgctcgcgcg ctacggcaca cccaaatcgc cggcggatct ggcgcgatgg ccgtgtgtga
      661 atttcgattt tttttcgccg gctccggtct ggccgtttcg acagcaaggc gccaaggccg
      721 ccatggagat gccgattcat ccgcgtctgt cggtttcaac cgcagaagcc gcagtggcgg
      781 cagcaacaga gggcgtgggc gcgacacgcg tgctgcacta tcaatgcgct gaggcgctta
      841 atgcgggcgc gctgcggatc atgctcgcgg attttgaact cgacccacta ccgattcatc
      901 tgcttcacgc cggccgtgcc gcgttgccct cgaagacgcg cgtattcctg gaattcgcga
      961 cggcgcgttt gcggcaaagg ctgaaacatt taccgcccgc agccaccaat gacgcgatcg
     1021 aagactagcg cccgtccgca tctcagcgcg agaggcgatg aaacaataaa ttcgatacgg
     1081 cgatcatttc gtcggggccg ccatatcttg ctctccccga aaccaagtcc gccaagctct
     1141 cccctgtctc cttggataat tccttggtca ggattgcgcg cacccattct gcaagccgcg
     1201 catgtgcgag atagctaacg gctgccctgt cttccaatat ccccttcacg ataccgcgtt
     1261 tgtaaagcca atagatcgtc tcattcgagc gggaagagag ttgttcgaat tcccgcgcgt
     1321 tgtgctgaag acaacagccg agcacctcgt cggggccgat tgtcgggcgt tcaaatgcat
     1381 caaaaagaac agcaatggct cgcaaaatct ggagcttgaa ttccacgatt ctatccattt
     1441 gttcctccat cgaatttcga cgaggaccca ggcgctgacg tgtttcgcta aactgataag
     1501 gccgcataag cggcagcgcc ggatgttaaa atgattacga gaataaaaat tgcgactctc
     1561 agccacgctc aagatgaagc gcctgaagcg gaatgcaaga aagctggcga gaaagttgga
     1621 tagttatcca ccgcggactt tccggatcga tccggcttgc cggttggtgc ttcacaaata
     1681 ttctcgtgca aaggctatga cttcaaggaa gatagaaggc gcacgcgagc acaatgaaga
     1741 gggccgcgcc gaattttaat tcctgccgta attcgaacga tcccgaagcc atggatgtcg
     1801 ccctggttat aacgacggcg agcagcgtcg cggcgatcat ccaccgtgaa atgtcgttcg
     1861 atagaaaatt tcttatcggg tctaaaataa tgtcccaaaa catggcggga cctctcgtca
     1921 ataaacccaa gcctgtcgca agaaaattta gccaaggcaa agccgatggc taaattctta
     1981 gatatcccaa cgaggatgcc attgatgggc aatgaaatgt aaattgctta tctcatacgt
     2041 gacaatcggg cggccagtgg tgcgttgcac catgtcaaat tgaatagatg atttcatgga
     2101 aacagccatt cttgcgtgat ggataagaac ggccgcggac aaattttaaa ggatcatttg
     2161 cgctgcgctg ccgagaactt cgcgaggagc gcgtcatgaa ttggtatggc aacgagacaa
     2221 ccaacacttg ggattcgcac ataaccaaag cgctcacatc agcttacgat gatgccttgg
     2281 cgcgcgccga aaaactcgga attctcggcg agaaaaccgg gcatgctgca tcggatatta
     2341 tcgcgcggca tatcatcgaa ggcgcgaaac gcgggaaata cgatccgcaa ctccttgccg
     2401 ccggcgctct tgaacgtctt caacgaggct gaattcgttt tcccgggata tattctgcga
     2461 gttcctagga actaccatac gccaccgggg cattctttgc cgctttcgga ataaattgct
     2521 gctttattcg gtacgattcc gtacttgctg acgcgggtct tgacgtcctt gggcctgtcg
     2581 catgtttgga atggagtaag ctcatgaaga agttgattgc ggccgcgctg ttcgcatctg
     2641 tcgttgtcgt tgccagcccg gcgttcgcga taacttttag cctgatccag gatctcgata
     2701 ccggaaactg cgctgccgtc gttacgtctc cgaacggctg gccgggaatg aaagccgtca
     2761 gcgaaacgac ctattcctct caggaggagg ccgacaaggc gctcgacagc gtgaaggctt
     2821 gcaaatcttt cgtccgctaa ttcgctcaga agaccgctgc gcttgtcggc ggtctttctt
     2881 ctacgaatga aaaatctccg ggggcggcag agccggtgcc tggcaaatgg tggcgggcta
     2941 tatcgatgag ccttgtcggc gcgatatttt aagcgggcct ataaattctg cggcgacggt
     3001 aaacgcttgg taactcgtgg gaccgatgaa cggtaatgcc ggatgaaacg gccacccaca
     3061 taatcgatcc cagattgggc tgccgctttc gatggcaaat agccgatcat acaacgccct
     3121 ctcgttcgag gttgcagctt aaatggcagc atgatgaagc gaagtccgct cggcacccgc
     3181 cggcgagggc ttttcgcgcg ttagaatttc ttcatcgcac aagattaagc aatagaagcg
     3241 cggttgccat ggtcgcgcat tatagggagt tatttatgaa aacgctcata actgggttgc
     3301 tcgccatcgc gatgctttcc gctagcttgt catcagtatt tgctctgggt ggttgcggtc
     3361 cgaaccgaca tcgcgcttat ggccattgcg tatggggcgg tcaaaatgaa ggttggtgcg
     3421 caagacatac gggccacact gccgttcgca tgcccaacgg aaacatgcgt tgcttcaggt
     3481 aattgccttc ggcggcgtgc gcttttgcgc cgcacataag aacccgccgg gctttcacgg
     3541 ccgggcgggt tcgtgcaagc gccggcaaac tggtctgcgc gcactttcga accattgact
     3601 tcgaggctcg cgagacggcg ggaccgggtt cgaaaccggt ttcagcctaa aatcttcaga
     3661 ttttccctag agagtctggc ggagaggggg ggattcgaac ccccgatagg cttgcaccta
     3721 tgccgcattt cgagtgcggt gcattcaacc gctctgccac ctctccagcg cgggttgcgg
     3781 tttgcaccgc ctagcacgca caccccggcc ttacaagact cggaaaaatt gccgccggag
     3841 agaaccacgg tgccggctga gccgtattcc tatgcggcgg gataggcgtc tcgtcgggag
     3901 gtttcaggtg aacacatccg accctacgga gcggccgggg agcgactggc cgaaaagtct
     3961 cgcgctcggc ttcgttttct tatggttctt tgtcggcggg atcgcgcatt tcgctttcac
     4021 cgcgcgcgag atggaaatcg tcccaccgcc atttccggca catcgcgcct tggtcctgat
     4081 gctgctcttc ctgatctatt ggtcgacacg tccgccggag gcagcttagc gccctattcc
     4141 ggctcgtcgc cctgcatgaa aaggccgaat tgctgcggcc gcgccggggc gtcgaggcca
     4201 agatgctcga aggcgcgggg cgtcagtacc cggccgcgcg gcgtccgttg cagaaagcct
     4261 tgctggatca gatagggctc gatgatatcc tcgatcgcgt cgcgcggctc agagagcgcc
     4321 gcggccagcg tttcgatccc gacagggccg ccaccaaagc tcgatgcgat caggccgaga
     4381 tagcgccggt ccatc
//
LOCUS       DASWOG010000073         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_276568,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000073 DASWOG010000000
VERSION     DASWOG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276568"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGB93_01465"
     CDS             <1..560
                     /locus_tag="VGB93_01465"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0144829.1"
                     /translation="LATLPIIIAGSLLSKLLNTCNSPLRSLAAIGVAYIVMGALLAIA
                     EVYSRHKRTLDHVDLKDAMIVGLAQCGALIPGVSRSGSTFTAALFRDLERTEAARFSF
                     LLGIPAIAAAGIKELYELHKAHLDAHGWSILAFGLVVASISAFIAIWGLLRILERFAS
                     WPFAIYRVLLGIFLLVAVAAHWLPN"
     gene            733..>1537
                     /locus_tag="VGB93_01470"
     CDS             733..>1537
                     /locus_tag="VGB93_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0144830.1"
                     /translation="MTDAAIKKPGSGIAGEFAESLGLITPLERAWHFRELIRAVVRRE
                     LMARFSGSILGWMWAIFGPLLMLSVYTVIFSHAVGIRASTASHGVANYSLNIFVGLIM
                     FNLFSELAYRAPGLLHEHVNFIKKSIFPSETLAWIAAIRALVYAGISFCVLLFFELLI
                     THWIPPTILLLPFVIVPFFLFLIGASWFLMALGSFTRDVMHLMASIVPVFMFATPIFY
                     SFDDVPPVLRVILRVNIVGDYVELMRDIVLLNRIPNPWLFLGTVIVSLLV"
BASE COUNT          231 a          495 c          418 g          393 t
ORIGIN      
        1 tgctcgcgac gctgccgatc atcatcgcgg gctcgttgct ctcaaaactt ctgaatacct
       61 gcaattcgcc gctgcgctcg cttgccgcga tcggcgtcgc ctatatcgtc atgggggcgc
      121 tgcttgccat tgcggaagtc tattcgcggc acaaacgcac gctcgatcat gtcgatctga
      181 aggacgcgat gatcgtcggc cttgcccaat gcggtgcgct catcccaggc gtttcccgct
      241 ccggctcaac cttcaccgca gcgcttttcc gcgacctcga acgcaccgaa gcggcgcgct
      301 tttccttcct gctcgggatc ccggccattg ccgcggcggg gatcaaagaa ctttacgaat
      361 tgcataaggc gcatctcgac gcccacggct ggtcgatcct cgcctttggt ctcgtcgtcg
      421 cgtcaatttc agccttcatc gcgatctggg gcctcctgcg tatcctcgaa cgcttcgcgt
      481 cctggccgtt cgctatctat cgggtcttgc tcggcatttt tctgctcgtc gcagtcgccg
      541 cccattggct gccgaattaa gtggcgggcg ctgccgtagg ccgcaaggtc tggtgccagg
      601 ggcgtcaccc gacaaatttt ttgccgccct ttacgccccc aaacggcatc acggtggcga
      661 cgcgcgccgg tttgactagg ccggcgcaaa ctggtctaca tccgcgtccg tttggggttt
      721 cacagagcca atatgactga cgccgccatc aaaaagccgg gctccggcat cgctggagaa
      781 tttgctgaaa gtctcggcct catcacgccg ctggaacggg cctggcattt ccgcgaattg
      841 atccgcgccg tcgtgcgccg cgagctgatg gcgcgcttca gcggctcgat cctcggctgg
      901 atgtgggcga tcttcggacc gctgctgatg ctgtcggtct acaccgtcat cttctcgcat
      961 gcggtcggca tccgggcttc gaccgcttcg catggcgtcg caaactattc gctgaacatt
     1021 ttcgtcggcc tcatcatgtt caacctgttc agcgaattgg cctaccgcgc gcccggcctg
     1081 ctgcacgagc atgtgaactt catcaagaag tcgattttcc cgagcgagac gctggcctgg
     1141 atcgcggcga tccgcgcgct cgtctatgcc ggaatcagct tttgtgtcct gctgtttttc
     1201 gaacttctca tcacgcactg gataccgccg acgattctgc tgctgccttt cgtcatcgtg
     1261 ccattcttcc ttttcctgat cggcgcgtcg tggtttttga tggcgctcgg ctcgttcacg
     1321 cgcgacgtga tgcatttgat ggcatcgatc gtcccggtgt tcatgttcgc cacaccgatc
     1381 ttttattcgt ttgatgacgt cccgccggtg ctccgcgtca ttctccgcgt taatatcgtc
     1441 ggcgattatg tcgagctgat gcgcgatatt gttctgctca accggatccc aaatccttgg
     1501 ctctttctgg ggactgtcat tgtctcactc ctcgtct
//
LOCUS       DASWOG010000074         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_313426,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000074 DASWOG010000000
VERSION     DASWOG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313426"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGB93_01475"
     CDS             <1..466
                     /locus_tag="VGB93_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY0144831.1"
                     /translation="TAHVETAAQMFAAVETALPADIFIATAAVADWRAEAAPQKIKKS
                     AAPPQFVFTENPDILAIVAQRKSARPRLVVGFAAETENLLVHAREKLQRKRCDLIVAN
                     DVGENGAMGKDENEVTLVSAAETEQWPKMRKDDIAAKLVRHLANMLKEGRDG"
     gene            459..962
                     /gene="dut"
                     /locus_tag="VGB93_01480"
     CDS             459..962
                     /gene="dut"
                     /locus_tag="VGB93_01480"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0144832.1"
                     /translation="MAEEEAPRVAIRLLPHGEGLPLPAYHSAGAAGADLCAAIAEDER
                     IIIEPGARHLVPTGLVLQLPEGYEGQVRPRSGIALHRGVTLLNAPGTIDSDYRGEVSV
                     LLINLGAEPFEIIRGARIAQLVVAPVTRVDFVEAADLTATTRGAGGYGSTGLGSSGKL
                     GAPGGNS"
     gene            959..1402
                     /locus_tag="VGB93_01485"
     CDS             959..1402
                     /locus_tag="VGB93_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0144833.1"
                     /translation="MMLLDRRSVLALAAVVDIAIHARPQPVAAKQLAGRLDLPPRHLE
                     TLLQTLVRADILKGVRGPRGGYELARERRRITAAEIVRAVQSEAADDAKPENAFVENI
                     ISPVVARAGEAFLTALEQVTIDDICGQVAETKTSREQAAKSDFTI"
     gene            1513..2502
                     /gene="cysK"
                     /locus_tag="VGB93_01490"
     CDS             1513..2502
                     /gene="cysK"
                     /locus_tag="VGB93_01490"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0144834.1"
                     /translation="MAQAAVKAAAGAAKTPGRGRIYDSIIETVGDTPLVRLDKIAKEK
                     GIKANLLAKLEFFNPIGSVKDRIGVAMIAAAEAEGKISPERSVIVEPTSGNTGIALAF
                     VAAAKGYRLILVMPESMSIERRKMLALLGAELVLTPAPQGMKGAIAKAQEISNATPGA
                     YTPQQFENPANPEIHRRTTAEEIWNDTHGEVDVFVSAVGTGGTLTGVAEVLKARKPGV
                     RIVAVEPEDSAVLSGGQPGPHKIQGIGAGFVPPILDRKLIDEVVTVGNQTSFDTARLV
                     ARLEGIPVGISSGAAVAAAVEVGARSEFAGKNIVIIIPSFAERYLSTALFDGL"
     gene            complement(2558..2845)
                     /locus_tag="VGB93_01495"
     CDS             complement(2558..2845)
                     /locus_tag="VGB93_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004961451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY0144835.1"
                     /translation="MVAYVVFTREKMRNPEEYERYREKARPAAEGHPLKPLALYGRYE
                     TLEGPAMEGAVVLEFPTIDAAKSYYNSPAYQDAVKHRFLGADYRVFIVEGL"
     gene            2961..3962
                     /locus_tag="VGB93_01500"
     CDS             2961..3962
                     /locus_tag="VGB93_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY0144836.1"
                     /translation="MTEPSLDILGIGNAIVDVIAPCDDSFLAAHSLNKGGMTLIDEAT
                     AEKLYDAMGQTIVVSGGSAANTIIGAAALGAHTGFIGKVKQDSLGQMFAHDIRAAKVA
                     FGTEPATDGAASARCLILVTPDGQRTMNTFLGACQALSEADIDPSQVAAAQITYLEGY
                     LWDPPAAKTAFAKAAEIAHGAGRRVAFSLSDSFCVDRYRDEFLHLIRSGAIDILLANE
                     SELHSLYQTADFKTAVAQLREEDILAAVTRSEKGALVVTREETQSINAFPIETLVDTT
                     GAGDLFAAGFLVGLSREQTLADCARLGALAAAEVIQHYGARPQADLKVLATENGLLD"
     gene            complement(3959..4936)
                     /gene="coaA"
                     /locus_tag="VGB93_01505"
     CDS             complement(3959..4936)
                     /gene="coaA"
                     /locus_tag="VGB93_01505"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEY0144837.1"
                     /translation="MAGRFMDERVTQPPAILSPYHRFTRSEWASLRADTPLTLTVEDL
                     NRLRSLSEPLSLEEVIEIYLPLSRLLALYVAATQGLYKATQRFLGSDGGKVPYIIGVA
                     GSVAAGKSTLARVMQALLLRWPNTPKVQLITTDGFLYPNAVLAAEGLMDKKGFPESYD
                     GMALLNFLSDVKAGTSSVRAPVYSHLTYDIMPDEFVTVDRPDILIVEGLNVLQPGRPP
                     KDGRGTAFVSDYFDFSVYLDADEDVLEQWYVERFQRLRQTAFRDPRSYFRKYADQTDA
                     QAEAMARDIWTRINLRNLHENILPTRARASLVLTKDASHSIQTVELRKL"
     gene            complement(5022..5342)
                     /locus_tag="VGB93_01510"
     CDS             complement(5022..5342)
                     /locus_tag="VGB93_01510"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY0144838.1"
                     /translation="MSQFTLADLAAIIVQRANADAKDSYTKSLLQAGPARAAKKLGEE
                     AVETVIAAIEGEPKALVSESADLLYHLLVVLQTRGVALDDVLAELERRTSQSGHEEKA
                     ARKG"
     gene            complement(5350..6135)
                     /gene="hisF"
                     /locus_tag="VGB93_01515"
     CDS             complement(5350..6135)
                     /gene="hisF"
                     /locus_tag="VGB93_01515"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0144839.1"
                     /translation="MLKARLIPCLDVKDGRVVKGINFVDLRDAGDPVACAIAYDAAGA
                     DELCFLDITASHENRGTLLDVVRRTAEACFMPLTVGGGVRAVEDIRNLLLAGADKVAI
                     NTAAVQNHAFVRAGAEKFGSQCIVVAIDAKQVSPRRWEIFTHGGRKPTGLDAIEYARE
                     VVSLGAGEILLTSMDRDGTKSGFDVALTRAVVDAVNVPVIASGGVGALNHLVEGIREG
                     GASAALAASIFHFGEFSIAEAKSYMAKHGVAVRLDGVDVAAAA"
     gene            complement(6128..6880)
                     /gene="hisA"
                     /locus_tag="VGB93_01520"
     CDS             complement(6128..6880)
                     /gene="hisA"
                     /locus_tag="VGB93_01520"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY0144840.1"
                     /translation="MAAVILFPAIDLKNGECVRLLYGDMDRATVFNKDPAAQAEAFER
                     QGFTYLHVVDLDGAFAGKPMNASAVDSILARVKMPVQLGGGIRDLRTVEGWLGKGIAR
                     VIIGTAAVRDPDFVREAARRFPGQIAVGIDARDGKVAVAGWAEATEMDVIELGRRFED
                     AGVAAIIYTDISRDGALKGLNVEATLALADAISIPVIASGGLASLADVERLLEPDCAK
                     LEGAITGRALYDGRLHPQAALALVRAAEKSLA"
     gene            complement(6868..7518)
                     /gene="hisH"
                     /locus_tag="VGB93_01525"
     CDS             complement(6868..7518)
                     /gene="hisH"
                     /locus_tag="VGB93_01525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0144841.1"
                     /translation="MNVAIIDYGSGNLHSAHKAFERAAREAGLNAEIKVTGDPEDVRR
                     ADHIVLPGVGAFADCRRGLDAVPGMIEALDERVRKDGQPFLGICVGMQLMATRGLEYE
                     TTNGLGWICGDVAAIEPSDANLKIPHMGWNTLAAEKAHPLLANIPTGPDGLHAYFVHS
                     FAYTNVPKDEIVASTDYGGPVVAIVGRDNIVGTQFHPEKSQKLGLALIANFLRWQP"
     gene            complement(7515..>7751)
                     /locus_tag="VGB93_01530"
     CDS             complement(7515..>7751)
                     /locus_tag="VGB93_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEY0144842.1"
                     /translation="LGLEGNSLRRWELARRGFRLTDITAGLRRSDAERSYFGRALRAI
                     PPPVPTPKISDAAPAFAPPPHEVVCLFPHPDDAR"
BASE COUNT         1459 a         2404 c         2439 g         1449 t
ORIGIN      
        1 taccgcgcat gtcgagacgg cagcgcagat gtttgctgct gtcgagacgg cgctgccggc
       61 cgatattttt atcgccactg cggccgtcgc cgattggcgc gcggaagctg ctccacaaaa
      121 gatcaagaaa tccgccgcgc cgccgcaatt cgtgttcacc gaaaatcccg acattctggc
      181 gattgtggcg cagcgcaaat ccgcgcgccc gcgcctcgtt gttggcttcg ccgccgagac
      241 cgagaatctg ctcgtccatg cacgcgagaa attgcagcgc aagcgttgcg atctgatcgt
      301 tgccaacgac gtcggtgaga atggcgcgat gggcaaagac gaaaatgagg tgacgctcgt
      361 ctccgccgct gaaaccgagc aatggccgaa aatgcgcaag gacgacatcg ctgccaaact
      421 cgtccggcat ctggcaaaca tgctgaagga aggccgcgat ggctgaagaa gaagcgcccc
      481 gcgtcgcaat ccgattgttg ccgcacggcg aaggcctgcc gctgccggcc tatcacagcg
      541 ccggtgcggc gggcgccgat ctctgcgcgg cgatcgcaga ggacgagaga atcattatcg
      601 agccgggtgc gcgccatctc gtcccgaccg gtctcgtctt gcaattgccg gagggctacg
      661 aaggccaggt gcgaccgcgc tccggcatcg cgttgcatcg cggcgtcacg ttgctgaacg
      721 cgccgggcac gatcgattcg gattatcgcg gcgaagttag cgtccttctg ataaatcttg
      781 gcgccgagcc cttcgaaatt atacgcgggg cgcgcatcgc ccaacttgtc gtcgcaccgg
      841 tgacgcgcgt ggactttgtc gaagcagcgg atctgaccgc gacgacgcgc ggcgccggcg
      901 gctacggttc gaccgggctt ggttcgtcgg gaaagcttgg cgcgccggga ggcaattcat
      961 gatgcttctg gaccgtcgca gtgtcctggc gcttgccgcg gtcgtcgata tcgcgattca
     1021 tgcgcgcccg cagccggtgg cggcgaagca gcttgccggg cggctcgatc tgccgccgcg
     1081 ccatctcgaa accttgctgc aaactctcgt gcgggccgac atcctcaaag gggtgcgtgg
     1141 cccacgcggc ggctacgaac ttgcgcgcga gcgccgccgt atcacggcgg cggaaattgt
     1201 ccgtgccgtt caaagcgaag ccgccgacga cgcaaaaccg gaaaacgctt ttgtcgaaaa
     1261 tatcatctcg cccgtcgtcg cgcgcgccgg cgaggccttt ttgaccgctc tggaacaggt
     1321 gacgatcgac gacatttgcg ggcaggtggc cgagaccaag acgagtcgcg aacaggcggc
     1381 gaaatcggat tttacaatct aacacagtat ataactttta tatacttccg aggctgcggg
     1441 aataatgata tggctccgcg cagcgtggat ttgcttggcc ttgtgggccg gttggcagac
     1501 aggagaaaca ggatggcgca agccgcagtg aaggcagccg caggcgcggc taagacgccg
     1561 gggcgcgggc gcatttatga ttcgatcatc gagactgtcg gcgatacgcc gctcgtgcgt
     1621 ctcgataaga tcgccaagga gaagggcatc aaggccaacc ttctggcaaa gctcgaattc
     1681 ttcaacccga tcggcagcgt caaggacagg attggcgtcg ccatgatcgc ggctgccgag
     1741 gccgagggca agatttcgcc ggagcgttcc gtcatcgtcg agccaacctc cggcaacacg
     1801 ggcatcgcgc tcgcgttcgt cgccgcggcg aagggctatc ggctcattct cgtcatgccg
     1861 gaatcgatgt ccattgagcg ccgtaagatg ctcgccctgc tcggcgccga actcgtgctg
     1921 acgccggcgc cgcaaggcat gaaaggcgcg atcgccaagg cgcaggaaat ctccaacgca
     1981 acgcctggcg cctacacacc gcagcaattc gagaatcccg ccaaccccga gattcatcgc
     2041 cgcaccacgg ccgaggaaat ctggaacgat acgcatggcg aggttgatgt cttcgtctcc
     2101 gccgtcggca ctggcggcac gctcaccggc gttgccgaag tcctgaaggc ccgcaagccc
     2161 ggggtgcgta tcgtcgcggt cgagcccgag gattccgccg tgctctcggg cggccagcct
     2221 ggcccacaca aaattcaggg cattggcgcc gggtttgttc cgccgatcct cgatcgcaag
     2281 ctgatcgatg aagtcgtgac agtcggcaat cagacatcgt tcgacaccgc gcgcctcgtc
     2341 gctcggctgg agggcattcc ggtcggcatc tcgtccggcg cggcggtggc cgccgccgtc
     2401 gaagttggcg cgcgcagcga attcgccggc aagaacatcg tcatcatcat tccgtccttc
     2461 gccgagcgct atctttcgac ggctttgttt gacgggctgt gatcctgctc tttggcgcga
     2521 ggtccggcgg ggtgtgtcac aatttgaatg gcacgcctca cagcccttcg acgatgaaca
     2581 cccggtaatc agccccgagg aaccgatgct tgaccgcatc ctggtaggcg gggctgttgt
     2641 aatagctctt cgctgcgtcg atggttggaa actccaagac aacagcgccc tccatcgccg
     2701 ggccttccag cgtctcgtat ctaccgtaaa gcgccagcgg cttcagagga tggccttctg
     2761 cggcaggccg ggctttttcc ctgtagcgct cgtattcttc cgggtttcgc attttttcgc
     2821 gtgtgaacac gacgtatgcg accatggtgt aaccctcctc atttcaagaa ttcatagcag
     2881 gatgctaccg ccgggcggcg ccgcttgacc ttggcgcgcg gcgatgcgaa aggctcgcct
     2941 cccgcgtgag cccatcctac atgaccgaac ccagcctcga tattctcggc atcggcaatg
     3001 ccatcgtcga tgtcatcgcg ccctgcgatg attcttttct tgcggcgcac agcctcaaca
     3061 aaggcggcat gacgctgatc gatgaggcaa cggcggaaaa actttacgat gcgatgggac
     3121 agaccatcgt cgtctctggt ggctcggcgg ccaatacgat catcggcgcg gcggcgctcg
     3181 gcgcccacac gggcttcatc ggcaaagtga agcaggattc gctcggccag atgttcgcgc
     3241 acgacatccg cgccgcgaaa gtcgcctttg ggacggagcc tgcaaccgat ggcgccgcca
     3301 gcgctcgctg cctcattctt gtgacgccgg acggccagcg cacgatgaac accttccttg
     3361 gcgcttgcca ggcattgagc gaagccgata tcgatccttc ccaggtcgcg gcggcgcaga
     3421 tcacttatct cgaaggctat ctctgggatc cgccggcggc gaagacggcc tttgcgaaag
     3481 ccgcggagat cgcgcatggc gccgggcgca gagtggcgtt cagcctgtcg gattcgtttt
     3541 gcgtcgatcg ttaccgcgac gaattcctgc atttgatccg ctcgggcgcc atcgacattc
     3601 tcttggccaa cgagagcgag ttgcattcgc tctatcagac agccgacttc aagaccgcgg
     3661 tggcgcaatt gcgcgaggaa gacattctgg cggcggtgac gcgcagcgaa aagggcgctc
     3721 tcgttgtcac gcgcgaggaa acgcaatcga tcaatgcgtt cccgatcgaa acgctcgtcg
     3781 atacgaccgg cgccggcgat cttttcgcgg cgggcttcct tgtcggtctg tcgcgggagc
     3841 agacgctcgc cgattgcgcc cggctcggcg cgctcgcggc ggcggaagtc atccagcatt
     3901 atggcgccag gccacaggcc gatctcaaag tgctcgcgac cgagaacggg ctgctggact
     3961 aaagcttccg caattcgacc gtctggatcg aatggctggc gtccttggtg agcacgaggc
     4021 tggcgcgggc gcgcgtcggc aagatgtttt cgtgcagatt gcgcaggttg atgcgcgtcc
     4081 agatatcgcg cgccatcgcc tccgcctgcg cgtccgtctg atcggcatat ttgcggaaat
     4141 aggatcgcgg atcgcgaaac gcggtctgcc gcagccgttg gaagcgctcg acataccatt
     4201 gctcgagaac gtcttcgtcg gcgtcgaggt agactgagaa gtcgaaataa tccgatacga
     4261 acgccgtgcc gcgtccgtct ttcggcggac gccccggttg caagacgttg agaccttcga
     4321 cgatcaaaat atcgggccgg tcaaccgtca cgaattcatc cggcatgatg tcataggtca
     4381 gatgcgaata gaccggggcg cggacactgc tggtgccggc cttcacgtca gacagaaagt
     4441 tgagcagcgc catcccgtcg taactttcgg gaaagccctt cttgtccatc aggccttccg
     4501 ccgcgaggac ggcgttagga taaagaaagc cgtcggtcgt aatgagctgg accttgggcg
     4561 tgttcggcca gcgcaacaga agcgcctgca tgacgcgcgc gagcgttgat ttgccggcgg
     4621 cgacggagcc tgcgacgccg atgatgtaag gcaccttgcc gccgtcagag ccgagaaagc
     4681 gctgcgtcgc tttataaagc ccctgcgtcg cggcgacata gagcgccagc aggcgggaga
     4741 gcggcaggta gatttcgatg acttcttcga gagacagcgg ctcgctgagc gaacgcagcc
     4801 tgttcagatc ctcgaccgtc agcgtcagtg gcgtatcggc gcgcagcgag gcccactcgc
     4861 ttcgcgtgaa gcggtgatag ggggaaagaa ttgccggcgg ctgcgtgacg cgctcgtcca
     4921 taaatctccc agccatgccg ggtccccgtg cgaggcttcg ggcatattag acgacaaatt
     4981 atttttttaa accggccgaa atggcaattg attgcgctct ttcaaccttt acgggcggct
     5041 ttttcctcgt gccccgattg gctggtgcgg cgttcgagtt ccgccagaac gtcgtcgagc
     5101 gccacgccac gcgtctgcaa gacgacgagc aggtgataga gcagatcggc gctttcgctg
     5161 acgagcgcct tcggctcgcc ctcgatggcg gcaatcacgg tttcaacggc ttcctcacca
     5221 agctttttcg ccgcgcgggc agggcccgcc tgtagcagcg acttggtata ggagtctttt
     5281 gcatccgcat tggcacgctg gacgatgatg gcggcgaggt ctgcgagggt gaactggctc
     5341 attctgtcct cacgctgccg cggcaacgtc aacgccgtcg aggcgaaccg cgacgccgtg
     5401 tttcgccatg tagcttttgg cttccgcgat actgaattcg ccgaaatgga agatcgaggc
     5461 ggcgagcgcg gcgcttgcgc ccccttcgcg aatcccctcg acgagatggt tgagcgcacc
     5521 gacgccgccc gaggcaatga ccggcacatt caccgcatcg accacggcgc gggtcagcgc
     5581 aacatcgaat cccgacttgg tgccgtcgcg atccatcgag gtgagcagga tttcaccggc
     5641 gccgaggctt acaacctcgc gcgcgtattc gattgcatcg agaccggtcg gcttgcggcc
     5701 gccgtgggtg aagatttccc agcgtctcgg cgacacttgt tttgcgtcaa ttgcgacgac
     5761 gatgcactgg ctgccgaatt tctccgcgcc ggcgcgcacg aacgcgtggt tctgcacggc
     5821 ggcggtgttg atcgcgacct tatcggcgcc tgccagaaga agattgcgga tatcctcgac
     5881 cgcgcgcacg ccgccaccga cggtcagcgg catgaaacag gcttccgcgg tgcggcggac
     5941 gacatcgagc agcgtgccgc gattctcgtg gctcgcggtg atatcgagaa agcacagctc
     6001 gtcggcgccc gccgcgtcat aggcaatggc gcaggcgacg ggatcgcccg cgtcgcgcag
     6061 atcaacgaaa ttgatgccct tgacgacacg gccgtccttc acgtcgaggc aggggatgag
     6121 gcgcgcttta agcaaggctt ttctccgcag cgcgaaccag cgccagcgcc gcttgcggat
     6181 gaagccgccc gtcataaagc gcgcggccgg tgatggcgcc ttcgagtttg gcgcaatcag
     6241 gctccaacaa gcgttcgaca tcggccagcg aggcaaggcc gcccgacgcg atgacgggaa
     6301 tcgagattgc gtcggcgagc gccagcgttg cttccacgtt caagcccttc aacgcgccgt
     6361 cgcgcgaaat atctgtatag atgatcgccg caacgccagc atcttcgaag cggcggccga
     6421 gttcgatcac gtccatttcg gtcgcttcgg cccaacccgc gaccgcaact ttgccatcgc
     6481 gcgcatcgat gccgacagcg atctggccgg ggaagcgccg cgccgcttcc cgcacgaaat
     6541 cgggatcgcg caccgcagcc gtgccgatga tgacgcgggc gatgcccttg ccgagccagc
     6601 cctcgactgt gcgtagatcg cgaatgccgc cgccgagctg caccggcatt ttcacgcggg
     6661 cgaggatgga atccacagcg ctcgcattca tcggcttgcc ggcgaaagca ccgtcgagat
     6721 cgacgacatg cagataggtg aagccctgcc gctcgaaggc ctcggcctgc gccgccgggt
     6781 ctttgttgaa caccgtcgcc ctgtccatgt cgccataaag cagacggacg cattcgccgt
     6841 tcttgagatc gattgcggga aacaggatca cggctgccac cggaggaaat tggcgatcaa
     6901 ggcgaggccc agtttctgac ttttctcagg atgaaattgc gtgccaacga tattgtcgcg
     6961 cccgacgatc gcgacgacag ggccgccgta atcggtcgaa gcgacaatct cgtccttcgg
     7021 cacgttcgta taggcaaacg agtggacgaa ataggcgtgc agcccgtcgg gtccggtcgg
     7081 aatattggcg agcagcggat gcgccttttc cgccgcgagt gtgttccagc ccatgtgcgg
     7141 gattttgagg ttcgcatcag acggttcaat cgcggcgaca tcgccgcata tccagccgag
     7201 gccattggtt gtttcgtatt cgagaccccg cgtggccatc aattgcatgc cgacgcaaat
     7261 gccgaggaac ggctgtccat ctttgcgcac acgttcgtcg agcgcctcga tcatgccggg
     7321 gaccgcgtcg agcccacgtc ggcaatcggc gaaagcgccg acgcccggca ggacgatatg
     7381 gtcggcgcgg cgcacatcct caggatcgcc cgtgactttg atctcagcat tcaggcccgc
     7441 ttcgcgcgcg gcgcgttcaa acgccttgtg cgccgagtgc agattgccgg agccataatc
     7501 gatgatcgcg acattcatcg cgcatcatcc ggatgcggaa acaagcaaac gacttcatga
     7561 ggtggcggcg cgaacgccgg tgccgcatcg ctgattttcg gcgttggcac cggtggcgga
     7621 atcgcgcgca aggcgcggcc gaaatagctg cgctcggcat cgctgcgccg aaggccggcg
     7681 gtgatatcgg tgaggcggaa gccgcgccgt gcaagttccc agcgccgcag gctgttgccc
     7741 tcaagtccga g
//
LOCUS       DASWOG010000075         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_349946,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000075 DASWOG010000000
VERSION     DASWOG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349946"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VGB93_01535"
     CDS             <1..1067
                     /locus_tag="VGB93_01535"
                     /inference="COORDINATES: protein motif:HMM:NF005687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0144843.1"
                     /translation="NWRKAGAIIIGRTNVRAFCSRFFTDNGLHGRTLNPWAPGRTPGG
                     SSGGAAAALACGIGPLAHGNDRAGSIRQPAYACGVTGIRPSLGRVPGLQQSEREPTLV
                     SQLCNMQGPLARSIADLRIGLEAMAVADPRGPWWCPVPFDLGECGPVRVALFDDLPGV
                     EIDPAVRGALRKAASYLEDCGYHIEEAAPPRFTEAARLFFTLVKTEERAGSSRLIDEF
                     GDDALRRARASTMATAESLSFEDYIAALARRTTILREWQLFMELYPLILMPVSWKLPA
                     PVDLDQTGDAAVAGMIASLHPLLAISVLGLPGLAVPTGFADGVPVGVQLVAGRFREDV
                     CFAAGEIIEAQCGPTPIWPR"
     gene            complement(1115..>2625)
                     /locus_tag="VGB93_01540"
     CDS             complement(1115..>2625)
                     /locus_tag="VGB93_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0144844.1"
                     /translation="IIVAAVYGIARIPTGFLPLEDQGYFIAAVQLPDGAWLDRTEAAM
                     EDVSDVLGKVDGIEHVVTVSGISVLDNSASLANAGVAYIILKPWNLRGAALGLLPMYK
                     NLNAALSKLQEARIIVVPPPPIQGVGNAGGFTFQLELKDGSFDLAKLQSITNNVVAAA
                     NGQSALQHVSSPFRATVPQLAIDVNRIEAETLHVSVQQVFDTLTSYLGSTYINQFNKF
                     GHTFQVYTQADSRFRVRPIDVENLPLRNQDGKMIPLGTVVHLEPDVGPSLISLYNLYP
                     SSTIIGQAAAGYSSGQSMDLMEQIAKRELPRGTGYDWTAMSYQEKIVGGQMYLAFGLA
                     LLLVYLVLAGQYESWLLPFAVILAVPLSLVGPVIVFSLLRIDNNLYVQIGLVLLIALS
                     AKNAILIVEFARDLHNEGKDIIEAGREAAKARFRPILMTSFAFILGVVPLVFAEGAGA
                     NARKSIGVTVFTGMLASTCLAVLFVPAFYVVIQTIRERHSAKKPPAAAAPVA"
BASE COUNT          502 a          783 c          849 g          491 t
ORIGIN      
        1 ccaactggcg taaggcgggg gccatcatta tcggccgcac caacgttcgg gcgttctgct
       61 cgcgtttctt caccgataac ggactacacg ggcgcacgct gaacccgtgg gcgccgggcc
      121 gcacacccgg tggatcgagc ggcggcgcgg cggcagcatt ggcctgcggc atcggcccgt
      181 tggcgcatgg caacgatcgg gccgggtcca ttcggcaacc ggcctatgcc tgcggggtca
      241 ccggcatacg tccctcgctc ggacgcgtgc cgggtctgca acagagcgaa cgcgagccga
      301 cgcttgtcag ccaactctgc aacatgcagg ggccgctcgc ccgcagcatc gccgatttgc
      361 gcatcggtct ggaggccatg gcggtcgcgg acccgcgcgg cccgtggtgg tgccctgtcc
      421 cgttcgatct cggcgaatgc ggacctgtgc gcgttgccct gtttgatgat ctacccggcg
      481 tcgaaatcga tccggcagtc cgaggggccc tacgcaaagc cgcatcatat ctggaagact
      541 gcggctatca tatcgaggag gctgcaccgc cgcgatttac cgaagcggcg cgtttgttct
      601 tcacgctggt gaagacggag gagcgggccg gctcatcgcg attgatcgac gagttcggcg
      661 atgacgcgct gcggcgcgcc cgtgcctcca caatggcgac cgccgagagc ttgtcgttcg
      721 aggattacat tgccgccttg gcgcgccgca ccacgatctt gcgcgaatgg cagctcttca
      781 tggagctcta tccgctgatc ctgatgccgg tctcgtggaa gctacctgcc ccggtcgacc
      841 tcgaccagac aggcgatgcg gccgtcgccg gaatgattgc atcgctccat ccgttgctcg
      901 ccatatccgt tcttggcctg ccgggcttgg ctgtgccgac gggcttcgcc gatggcgtgc
      961 cggtcggcgt acaactcgtc gccggccgtt ttcgagaaga cgtctgtttc gctgccgggg
     1021 aaatcatcga ggcgcaatgt ggcccgacgc cgatctggcc taggtaacgg cgaccttggc
     1081 ggcggacacg aacctgcaaa gacgggttcg aggcttaggc taccggcgcc gcggctgccg
     1141 gaggcttttt cgccgaatgg cgctcgcgga tcgtttgtat gacgacatag aaggccggca
     1201 cgaacaggac cgcaaggcat gtcgaggcca gcattccggt gaagacggtg acgccgatcg
     1261 atttgcgggc attggcgccg gcaccttcgg cgaagacaag cggcacgaca ccgaggatga
     1321 aagcaaagga cgtcatcagg atgggacgga accgtgcctt cgcggcttcg cgcccggcct
     1381 cgataatgtc tttaccctca ttgtgcaggt cacgggcgaa ttcgacgatc aggatggcgt
     1441 tcttggcaga cagagcgatc agcagcacaa ggccgatctg cacataaagg ttgttgtcga
     1501 tccgcagcag gctgaagacg atgacgggac cgacgagcga gagcggcacg gcgaggatga
     1561 cagcgaaggg caaaagccag ctttcatatt ggccggcgag cacgagatag acgagcagca
     1621 acgccagccc gaaggcgaga tacatctgtc cgccgacgat cttctcctga taggacattg
     1681 ccgtccaatc gtagccggtg ccccgtggca attcgcgttt ggcgatctgc tccatcaggt
     1741 ccatcgattg cccggagctg taacccgcgg cggcctggcc gatgatcgtc gatgacggat
     1801 aaagattgta aaggctaatg agcgaggggc cgacgtccgg ctccagatgc acgacggttc
     1861 cgagcgggat catcttgccg tcctgattgc gcagcggcag attttcgaca tctatcggcc
     1921 gcacgcggaa acgcgagtcc gcctgggtat agacttggaa ggtgtggcca aatttgttga
     1981 actgattgat ataggttgag ccgagataag aggtcagcgt atcgaagact tgttgcacgg
     2041 aaacgtgcaa ggtctcggcc tcgatgcggt tcacgtcgat ggcgagttgc ggcacggtcg
     2101 cgcggaacgg cgacgagacg tgctgcagtg cgctttggcc atttgcggcc gctacaacat
     2161 tgttggtgat actttgcaat ttcgccagat cgaaactgcc atccttcaac tcaagctgga
     2221 aggtgaagcc gccagcgttg ccgacaccct ggatcggcgg cggcgggacg acgatgatcc
     2281 gcgcctcctg aagcttcgac agtgcggcgt tgagattctt gtacatcggc aggaggccga
     2341 gcgccgcgcc gcgtaggttc cacggcttca agatgatata ggcgacgccc gcgttggcga
     2401 ggctggcgct gttgtcgagc accgaaatgc cggacacggt gacgacgtgt tcgatgccat
     2461 cgaccttgcc gagcacgtcg ctcacatcct ccatcgccgc ttcagtacga tcgagccaag
     2521 cgccgtccgg caattgtacg gctgcaataa aatagccctg atcttcgagc ggcagaaagc
     2581 ccgtcggaat gcgcgcgatg ccatagactg ccgcgacaat gatca
//
LOCUS       DASWOG010000076         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_349956,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000076 DASWOG010000000
VERSION     DASWOG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349956"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGB93_01545"
     CDS             complement(<1..579)
                     /locus_tag="VGB93_01545"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0144845.1"
                     /translation="MASEHFDVLIVGAGLSGIGAGYHLQRKCPGKSYAILEGRDCIGG
                     TWDLFRYPGVRSDSDMFTLGYSFKPWTEPKAIADGPRILNYVRETAADNGIDRKIRFN
                     HRVKRASWSSPEARWTVEVERGPVGEGAPQIVRFTCSFLFMCSGYYKYEQGYSPEFPG
                     SEDFAGRIVHSQQWTDDIDYAGKRVVVIGSGAT"
     gene            complement(641..1583)
                     /locus_tag="VGB93_01550"
     CDS             complement(join(641..1246,1248..1583))
                     /locus_tag="VGB93_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0144846.1"
                     /translation="MPRAYSLDLRERVVALVASGEPCRAVAELFDVSVASVVKWSQRA
                     RVTGSAAAKPMGGKRPYLLEGERDWLLARLAEKPDLTLHGLLAELGERGVTVSCDTLW
                     RFLKRQGISFKKTVFATEQDRPDIARRRAWWKRHQLKMDPARLVFIDETWAKTNMART
                     HGWWRRGSPLKAQVPHGHWRTMTFLAALRHDRIAAPCVIDGPINGESFRAYVEQILVP
                     TLRPGDIVVMDNLGSHKGIAIRRAIRAVDAHLVFLPPYSPDLNPIEQVFAKLKLLLRK
                     AEERTIEGVWRRIGSLLQNFTPQECANYLRNAGYASI"
BASE COUNT          322 a          525 c          526 g          329 t
ORIGIN      
        1 cgtcgcgcct gagccgatca ccaccacgcg cttgccggcg tagtcgatat cgtcggtcca
       61 ttgctgcgaa tgcacgatgc ggccggcgaa atcttctgag cccggaaatt ccggcgaata
      121 gccttgttcg tatttgtaat agccggagca catgaacagg aagctgcagg tgaagcgcac
      181 gatctgggga gccccttcgc cgaccggccc gcgctcgact tccacggtcc agcgcgcctc
      241 cggcgacgac cacgacgccc gcttgacccg gtggttgaag cggatcttcc tgtcgatgcc
      301 gttatcggcg gcagtctcgc ggacgtagtt cagaatccgc ggcccgtccg cgatcgcctt
      361 cggctcggtc cacggcttga acgaatagcc gagcgtgaac atgtcgctgt cggagcggac
      421 gccgggatag cggaacaggt cccaggtgcc gccgatgcag tcgcggccct cgagaatggc
      481 gtagctcttg cccggacatt tccgctgcag gtggtaaccc gcgccgatgc cggacaggcc
      541 ggcgccgacg atcagcacat cgaaatgttc cgatgccatg gtttgctccc gaggcaaatg
      601 tccgccgttt ctgattgcgt ccagttagag tctttctgat ttagattgaa gcatagcctg
      661 cgttgcgcag atagttggcg cattcctgcg gcgtgaagtt ctggagcagg ctgccgatgc
      721 gtcgccaaac gccttcgatg gtgcgctcct cggccttgcg gagcaggagt tttagctttg
      781 cgaagacttg ctcgatcgga ttgaggtcgg gactgtaagg cggcaggaag accagatgcg
      841 cgtcgacggc acggatagcc ctgcggatcg cgatcccttt gtgggatccg agattgtcca
      901 taacgacgat atcgccgggt cggagtgtcg gcaccaggat ttgctcgaca taggcgcgga
      961 agctctcgcc attgatcggc ccgtcgatga cgcagggcgc ggcgatgcgg tcatgccgga
     1021 gtgccgcaag gaaggtcatc gtgcgccaat ggccgtgcgg gacctgagcc ttgagtgggg
     1081 acccacgtcg ccaccagcca tgggtacggg ccatgttggt ctttgcccac gtctcatcga
     1141 tgaagacgag acgagcagga tccattttaa gctggtgccg cttccaccag gcgcggcggc
     1201 gggcgatgtc agggcgatcc tgctcggtcg cgaacacggt tttttttgaa gctgatgcct
     1261 tggcgcttga ggaatcgcca gagcgtatcg catgacaccg tgacgccgcg ttcgccaagc
     1321 tccgccaaca gcccatgaag ggttagatca ggcttctcgg ccaaccgggc cagcaaccaa
     1381 tcgcgctcgc cctccagcag ataaggacgc ttgccgccca ttggcttggc agccgcgctg
     1441 cccgtcaccc gcgctcgctg cgaccacttc acgacgcttg caacgctgac gtcaaacagt
     1501 tcagccaccg cgcggcacgg ctcgccgctt gcaaccaacg cgaccacacg ttctcgcaga
     1561 tccagagagt aagctcgcgg cattcatgct ggcctccgcc tccagcatga agcttgaatc
     1621 agagcgccgc tgatttggga atcccccgat tcaaataaaa tcggaaagac tctagaggat
     1681 atcgaagcga tccggtccgc cg
//
LOCUS       DASWOG010000077         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_38333,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000077 DASWOG010000000
VERSION     DASWOG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38333"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGB93_01555"
     CDS             complement(<1..457)
                     /locus_tag="VGB93_01555"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0144847.1"
                     /translation="MLEQLTHLRALAARHAEGRSKETAIPRVAIHKGCGPTQNSSALF
                     EPKLCLVLQGKKQIMIGDQLLYYDSANYFIASVELPATGRIIEASPERPYLGLTLTLD
                     WQNLAALIADTTERREPPGMGFCVSPVTPQLLDAWLRLMSLHETPDDIPV"
     gene            610..1338
                     /locus_tag="VGB93_01560"
     CDS             610..1338
                     /locus_tag="VGB93_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0144848.1"
                     /translation="MTELKTALVTGANKGIGYEVVRRLAKDGFTVWLGARDKPRGEAA
                     AAALTREGLDVRLLQIDVADEASVAKAARDLERQTGRLDVLINNAGIAAGFHTPPSEE
                     TVATIKAVYEVNVFGPVRTTQAFLPLLKAAPAPRIVMVSSGLASLTRLADPASEFYAF
                     NLLSYNSSKTALNAITVAFAKELAAAGFKVNAADPGYTATDLNGNTGYRTVEQAAEVI
                     IRLATLPADGPNAGFFDDQGPEAW"
     gene            1364..2326
                     /locus_tag="VGB93_01565"
     CDS             1364..2326
                     /locus_tag="VGB93_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144849.1"
                     /translation="MSKTFGAKSTTDEVLEGVDLSGKRVLVTGVSAGLGIETARTLAA
                     HGAQVVGAARDLAKAHSATAEVRAQAAHGGGLELIELDLASLASVRAAADALVADGRP
                     FDLVIANAGVMACPKSFTKDGFEMQFGTNHLGHFVFVNRIISLFKPGSRLVNLSSAGH
                     RYADVDLNDPNFEHTPYSEFIAYGRSKTANILFSVEFDRRHKDAGIRATAVHPGGILT
                     ELARHLPPNGLQERLDQINAERPAGSPPFELKTVPQGTATTVWAGVVAKADAVGGRYC
                     EDCHVAEVVEGETLMLGGVRSYALDPEHAKALWAKSEEMVGETF"
     gene            complement(2357..3667)
                     /locus_tag="VGB93_01570"
     CDS             complement(2357..3667)
                     /locus_tag="VGB93_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEY0144850.1"
                     /translation="MHGVHAEISTLNLWIAGAIVIFCILMAAFFNGSETALTAASRAR
                     MYSLQKSGDANAARVNRLLSRRDRLIGAILLGNTLVSIGSSAFLTSVLEVLFGYRGVL
                     YATGLMTFLLLVFAEILPKTLAISYPDRFALIVAPVIGFFTAIFGPILAAIETLLQGL
                     LKLVGIDTRVEHALLSGHEELKSTVDLLHHEGGVQRSERDMFGGLLDLRELTVEDAMV
                     HRTKMYTIDGDLPPRDFLRAVVESPFSRVPVWRSEPDNIIGLLHAKDLLRAIEDSSGD
                     LGDIKIDDVVLDAWYVPNTTTLQDQLQAFLKRKSHFALVVDEYGVVLGLVTLEDILEE
                     IVGDISDEHDPVVLGIRQNADGSITVDGSVPIRDLNRVMGWQLPDEEATTIAGLVIHE
                     ARAIPEMGQIFTFHNFRFEVLRKTRNRLAALRITPISAAAQAQA"
     gene            complement(3684..>5258)
                     /gene="aroB"
                     /locus_tag="VGB93_01575"
     CDS             complement(3684..>5258)
                     /gene="aroB"
                     /locus_tag="VGB93_01575"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0144851.1"
                     /translation="AEVERAADMPIPEIFARYGEPYFRDGERRVMARLLTAGPRVIAT
                     GGGAFMSEETRDRIKENGISIWLKADLDVLWRRVRKRNHRPLLQNGDPEGVLKRLMDE
                     RYPVYAEADLAIVSQDGPHDSVVVDLLVTLHKYLCADGVEDELVSEVQEKPAIFQRVN
                     VELGSRSYDILIGEAILPQIAAYAAGLAPGAAVAIVTDENVARTHLPAVAASLADARI
                     RHESVVVAPGEDSKSYATFEKVCDAIIAGRFERGDLVVALGGGVVGDLAGFAAASVRR
                     GMRFIQVPTSLLAQVDSSVGGKSGINSPHGKNLVGAFHQPRLVLIDTQVLRTLPLREF
                     RAGYAEVIKYGLINDRAFFEWLEDHWQGVFAGGADQVYAIRKSCAAKAAIVAADEHEA
                     GERALLNLGHTFAHAFERLVHYDNRRLVHGEAVAIGLACAYRFSAARGLLSAADAECV
                     TNHLRDVGLPAQIRDIPDWNIGADVIVEAMLQDKKVQRGVLTFILAHGIGEAFIARDI
                     AAADVRAFLAGELHAA"
BASE COUNT         1042 a         1614 c         1655 g          947 t
ORIGIN      
        1 gcacggggat gtcatcgggc gtctcgtgga ggctcatgag gcgcagccac gcatcgagaa
       61 gctgcggcgt caccgggctc acgcaaaacc ccatccccgg cggttcgcgg cgctcggtcg
      121 tatcggcaat cagcgctgcg agattttgcc aatcgagggt cagggtgaga ccgagatagg
      181 gacgctccgg gctcgcctcg atgatccgcc ctgtggccgg caattccacg gacgcgatga
      241 aataattcgc ggaatcgtaa tagagaagct gatccccgat catgatctgc ttcttgccct
      301 gcaggacgag gcagagtttc ggctcgaaaa gcgcggacga attttgcgtc ggcccgcagc
      361 ccttgtggat cgcgacgcgc ggaatggccg tctctttgct ccggccctcg gcatgccgcg
      421 cggccagggc gcgcaggtga gtcagttgtt caagcataag atgaacattg gcgcaccctg
      481 cccgcggggc aaggcatatt cggccccgtg tgccgctttt tgagaggatc aggcaagaga
      541 tcgacgcgat cgcgggcgcg aaacgcgacc cgcgccctca gatatcggtc acacgaaggg
      601 aaatcctgca tgaccgaact aaaaaccgcg ctggtcaccg gcgccaataa gggcattggc
      661 tatgaggtgg tccgccgcct tgccaaagat ggtttcacag tctggctggg cgcccgcgac
      721 aagccgcggg gcgaggctgc ggccgcggca ttgacgcgcg aggggctcga tgtccgtctc
      781 ctgcagattg atgtcgcgga tgaggcaagc gtcgcgaagg cggcgcgcga tcttgagcgc
      841 cagacgggtc ggctcgatgt cttgatcaac aatgccggga tcgcggccgg ttttcacacg
      901 ccgccaagcg aggaaacggt cgcgacgatt aaggcggtct atgaggtcaa tgtctttgga
      961 ccggtgcgga cgacgcaggc gttcctgccc ttgctcaagg cagcgcctgc gccgcggatc
     1021 gtgatggtga gcagcggcct cgcatcgctc acgcgcctag ccgatcccgc gagcgaattc
     1081 tatgcgttca atctgctcag ctacaattcc tcgaaaacgg cgctgaacgc gatcacggtc
     1141 gcattcgcca aggaactcgc tgccgccggc ttcaaggtca atgcggcgga cccgggctac
     1201 accgcgacgg acctcaacgg caataccggc tatcgcaccg tcgaacaggc cgccgaagtc
     1261 attatcagac tcgcgacgct ccccgccgat gggccgaacg cgggattttt cgacgatcag
     1321 ggccctgaag cctggtgaaa ctgaaccttc aatggagaat catatgtcca aaacattcgg
     1381 cgcgaaatcc acgaccgacg aagtgctcga aggtgtcgat ctcagcggta aacgcgtcct
     1441 cgtcaccggc gtttccgccg gtctgggcat cgagacggcg cgcacgctcg cggcgcacgg
     1501 agcgcaggtc gtgggcgcgg cccgcgatct tgccaaggcg catagcgcaa ctgcggaagt
     1561 acgcgctcag gccgcgcacg gcggcggtct cgaactgatc gaactcgatc tcgcctcgct
     1621 cgccagcgtt cgtgccgccg ccgatgcgct cgtggccgat ggccggccgt tcgatctcgt
     1681 catcgccaat gccggtgtta tggcctgccc aaagagcttc acgaaagacg gtttcgagat
     1741 gcagttcggt accaatcatc tgggccattt cgtcttcgtc aatcggatca tctcgttgtt
     1801 caaaccggga tcgcggctcg tcaatttgtc gtcggcggga catcgctatg ccgatgtcga
     1861 tctcaacgat ccgaatttcg agcatacgcc ctatagcgag ttcatcgcct atggccggtc
     1921 gaagacggcg aacattcttt tctcggtcga gttcgaccgg cgtcacaagg atgctggcat
     1981 tcgtgcgacg gcggtgcatc cgggcggcat cttgacggag cttgcccgcc atcttccgcc
     2041 gaacggactt caggaacggc tcgaccagat caatgcggag cgtcccgcgg gctcgccgcc
     2101 gtttgaactg aagaccgttc cgcaaggtac cgcgacgacg gtctgggccg gggtcgtggc
     2161 aaaggcggac gctgtcggcg gacgctattg cgaagattgc catgtggctg aggtggttga
     2221 gggcgagacg ctgatgctcg gcggcgtacg gtcctacgcg ctcgacccgg aacatgcgaa
     2281 agcactctgg gccaagagcg aggaaatggt cggcgaaacc ttctgagccg aaatcggcgg
     2341 cggtcaggct tagcggtcag gcttgcgcct gggccgccgc actgatcggt gtgatccgca
     2401 gcgccgcgag gcggttgcga gtcttgcgca aaacctcgaa gcggaaattg tggaaggtga
     2461 atatctgccc catttccgga atcgcgcggg cttcgtgaat gacgaggccc gcaatcgtcg
     2521 ttgcttcctc atccggtaat tgccagccca tgacgcggtt gagatcgcgg atcggcaccg
     2581 agccatcgac ggtgatcgag ccgtcggcat tctgtcgtat gccgagcacg acgggatcgt
     2641 gctcgtcgga aatgtcgcca acaatctctt cgagaatgtc ttcgagcgtg acgagcccaa
     2701 gcacgacgcc atattcgtca acgacgagcg cgaaatgcga cttgcgcttg aggaacgcct
     2761 gcaattgatc ctgcagggtc gtggtgttcg ggacgtacca ggcgtcgagc acgacatcgt
     2821 cgattttgat gtcgccgaga tcgccgcttg aatcctcaat ggcgcgcaga aggtcctttg
     2881 cgtgcaacag accgatgatg ttatcgggtt cgctgcgcca caccggaacg cgcgagaacg
     2941 gcgattcgac gaccgcgcgg agaaaatcgc gcgggggcag atcgccatcg atcgtgtaca
     3001 tttttgtccg atgcaccatc gcgtcctcga cggtgagttc gcgcaggtcg agcagtccac
     3061 cgaacatatc gcgctcggaa cgctgcacgc cgccctcatg atgcagaaga tcgacggtgc
     3121 tcttaagttc ctcgtggccc gataaaagcg cgtgctcgac ccttgtgtcg atgccgacaa
     3181 gcttgagcag gccctggagg agtgtctcga tcgcagccag aatcggtccg aaaatggcgg
     3241 tgaaaaagcc tatgaccggc gcgacgatca gcgcgaaacg gtcaggatag ctgatcgcaa
     3301 gcgtctttgg caggatttcc gcaaagacga gcagcaggaa cgtcatcagg ccggtggcgt
     3361 aaagaacgcc ccgataaccg aagagcactt cgagcacgct cgtcaggaac gccgaagagc
     3421 caatgctgac cagcgtgttg ccgagcagga tcgcgccgat gagccgatcg cgccggctaa
     3481 gcaggcggtt gacacgcgcg gcattggcgt cgccgctctt ttgaagcgag tacatgcgcg
     3541 cccgcgaggc tgcggtgagc gccgtctccg aaccgttaaa aaaggccgcc atcagaatgc
     3601 agaagatgac gatggcgccg gcgatccaga gattgagcgt cgatatctcg gcgtgaacgc
     3661 cgtgcatgat tgaaagtccc gtcttacgcc gcgtgaagct cgccggcgag aaaagcgcga
     3721 acatccgccg cagcgatatc gcgcgcgatg aacgcttcac cgatgccgtg cgccagaatg
     3781 aatgtcaaaa cgccgcgttg cactttcttg tcctgtaaca tggcctcgac gatcacatct
     3841 gcgccgatat tccagtcagg aatatcgcgg atctgcgccg gcagcccgac gtcgcgcaga
     3901 tgatttgtaa cacactctgc atccgccgca ctcaacaggc cccgcgcggc cgaaaagcga
     3961 taggcgcagg cgagcccgat cgccaccgcc tcgccatgca ccagccggcg gttgtcgtaa
     4021 tgaacgagcc gttcgaaagc gtgcgcaaaa gtgtggccga gattgagcag cgcccgctcg
     4081 cccgcttcat gctcgtcggc agcgacgatc gcggccttgg cggcgcagct cttgcggatc
     4141 gcgtagacct gatccgcgcc tccggcgaat acgccctgcc aatgatcttc cagccattca
     4201 aagaaggcgc ggtcgttgat gaggccgtat ttgatgacct cggcataacc ggcgcggaat
     4261 tcacgcaacg gcaaggtccg caaaacctgt gtatcaatca ggacgaggcg cggctgatgg
     4321 aaggcgccga cgagattttt gccgtgcggc gaattgatac cgctcttgcc gccgacggat
     4381 gaatcgactt gcgccagtag cgaggtcggc acctggatga aacgcatgcc acggcgcacg
     4441 ctcgccgccg cgaacccggc gagatcgccg accacaccgc cgcccagcgc gacgacaaga
     4501 tcgccccgct cgaagcggcc ggcgataatg gcatcacaga ctttctcgaa cgtggcgtag
     4561 gatttcgaat cttcgcccgg cgcgacgacg acgctttcat gccggatcct ggcgtccgcg
     4621 agcgaggcgg caaccgcggg cagatgcgtg cgcgcgacat tctcgtcggt gacgatggcg
     4681 accgcagcgc cgggggcgag cccggccgca taggcggcga tctgcggcag gatcgcctcg
     4741 ccgatcagaa tgtcatagct gcgcgatccg agctcgacat tgacgcgctg gaagatcgcc
     4801 ggcttttctt gcacctcgct caccaattca tcctccacgc catcggcgca tagatatttg
     4861 tgcaatgtga cgagcaaatc cacgaccacg gaatcgtgcg ggccgtcctg ggagacgatc
     4921 gcgagatcgg cctccgcgta gaccggatag cgctcgtcca tcagcctttt cagcacgccc
     4981 tcgggatcgc cgttctgcag tagcgggcgg tggttgcgct tgcgcacgcg ccgccagagc
     5041 acgtcaaggt cggctttgag ccagatcgag atgccgtttt ccttgatcct gtcccgcgtc
     5101 tcctcgctca tgaaagcgcc gccgcccgtc gcgatgacgc gcgggccggc ggttagcagc
     5161 cgcgccataa cacggcgctc gccatcgcga aaatacggct cgccatagcg ggcgaatatt
     5221 tccggaatgg gcatgtccgc agcgcgttcg acttcggc
//
LOCUS       DASWOG010000078         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_38334,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000078 DASWOG010000000
VERSION     DASWOG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38334"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="tyrS"
                     /locus_tag="VGB93_01580"
     CDS             <1..1173
                     /gene="tyrS"
                     /locus_tag="VGB93_01580"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0144852.1"
                     /translation="DEKAKSGDLVAYIGFDCTAASLHVGSLVQIMMLRWLQKTGGKPV
                     ALMGGGTTRVGDPSGKDESRKLLTEETIEANKAGIKQVFAKFLTFGVGKHDAIMADNA
                     DWLLGLNYVNFLRDVGRHFSVNRMLAMDSVKLRLEREHELSFLEFNYMCLQAYDFVEL
                     NRRHNVTLQMGGSDQWGNIITGIDLGRRLGRPQLYALTSPLLATASGAKMGKTAQGAV
                     WLDQNMLSIYDYWQYWRNCEDGDVARFLKLFTGLPLSEIERLAALRDADINEAKKILA
                     TEATAMVHGRTAAEQAAETARQTFEDGLAAASLPSVDVAREELETGLGVLSAFVKAGL
                     VASTGEARRQVKGGGLKINDATVTDERATLNSDDLIDGAIKLSLGKKKHVLLKPGA"
     gene            complement(1190..>2243)
                     /locus_tag="VGB93_01585"
     CDS             complement(1190..>2243)
                     /locus_tag="VGB93_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEY0144853.1"
                     /translation="QVRLSPGDDAHVDLSKSGDTVKVVIRAASFDGRPYLKTLTLSHA
                     DEIVGTEAAPHKTKPRAAAHVLAKTASDLNIDVAMKADKLTGFNDVAMTNVDLHLVKD
                     SQTLKQFSLTGRFGRRATIVGHLDHAGTPSPQLDLTTDNAGGLLAFVDLYKHMKGGSL
                     NVAMRLGKDAMAGSFVVNNFVLADEPALRRLVTENVPRNTEQAGTPQFDPDAVPFRHL
                     QVNFQRAESRLDISDGVIYGSQIGVTANGWLDFAAGRVDLQGTFVPAYDVNNFFTKIP
                     FFGLFAGGTNEGLFAVNYHITGSPRQPTLNINPFSIAPGILRKIFGSIDLSSPDNSAE
                     PSPGATPPMPGEEDGR"
BASE COUNT          464 a          650 c          691 g          438 t
ORIGIN      
        1 gacgaaaagg ccaagagcgg cgatctggtc gcctatatcg gcttcgattg cactgcggcc
       61 tcgctgcatg tcggctcgct cgtccagatc atgatgctgc gctggctgca gaagactggc
      121 ggcaaaccgg ttgcgctgat gggcggcggc accacacgcg tcggagatcc ttcgggtaag
      181 gacgaaagcc gcaagcttct gaccgaagag acgatcgagg ccaataaagc cggcatcaag
      241 caggtctttg ccaaatttct gaccttcggc gtcggaaagc acgacgccat catggccgac
      301 aacgccgact ggctgcttgg cctcaactac gtgaattttc ttcgcgacgt ggggcgccac
      361 ttttccgtca accgcatgct ggcgatggac tccgtcaagc tgcggctcga gcgcgagcac
      421 gaactctcgt tcctcgaatt caattacatg tgcctgcagg cttatgattt cgtcgaactg
      481 aatcgccgcc acaacgtcac gctgcagatg ggcggctccg accaatgggg caatataatc
      541 accggcatcg atcttggccg acggctcggc aggccgcagc tctacgcgct gacctcgccg
      601 ctgctcgcca ctgcctccgg cgccaagatg ggcaagacgg cgcagggggc tgtatggctc
      661 gatcaaaaca tgctgtcgat ctacgattat tggcaatatt ggcgcaattg cgaggacggc
      721 gacgttgcgc gcttcctgaa gctgttcacg ggattgccgc tcagcgaaat tgagcggctc
      781 gccgcgttgc gcgatgccga catcaacgaa gcgaagaaaa ttctcgcgac ggaagcgacg
      841 gcgatggtcc atggccgcac tgcggcagaa caggccgctg aaacggcgcg gcagaccttt
      901 gaagacggcc tcgccgccgc cagtctgccg agtgtcgatg tcgcccgcga agaactcgaa
      961 accgggcttg gcgtgctctc agctttcgtc aaagccgggc ttgtcgcctc gaccggcgag
     1021 gcgcgtcggc aggtcaaagg tggtgggctg aaaatcaacg atgcgaccgt gaccgacgag
     1081 cgtgcgacgc tcaattccga cgacttgatc gacggcgcca tcaaactttc gctcggcaag
     1141 aagaagcatg tgttgttgaa gccgggcgca taaacggcac cgcttcacac tatcggccgt
     1201 cttcctcgcc cggcattggt ggtgtcgctc ccggcgacgg ttcagcggaa ttgtccgggc
     1261 tgctcaaatc gatcgatccg aaaatcttgc gcaggattcc cggcgcaatg gagaacggat
     1321 tgatgttcag tgtcggctga cgtggcgacc cggtgatgtg ataattgacc gcaaagagcc
     1381 cttcgttcgt gcccccggcg aaaagaccga agaacggaat tttcgtgaag aaattgttca
     1441 cgtcataggc tggcacgaaa gtgccttgca gatcgacacg gccagcggcg aaatcgagcc
     1501 agccattcgc agtgacacca atttgcgagc cgtagatgac gccgtcgctg atatcgaggc
     1561 ggctctcggc gcgctggaag ttcacttgta gatggcgaaa tggcacggca tcgggatcga
     1621 attgcggtgt accggcctgt tccgtattgc gcggtacgtt ctcagtcacc agtcgacgca
     1681 aagccggttc gtcggcaagt acgaaattgt tgacgacgaa cgagccggcc atcgcatcct
     1741 tgcccagccg catcgcaacg ttgagcgaac cgcccttcat atgtttgtag agatcgacaa
     1801 acgcgagaag gccgccggcg ttatcggtcg tcagatcgag ctgcggcgac ggcgtaccgg
     1861 cgtggtcgag atggcctacg atcgtcgcgc gccggccaaa acggccggtg agcgagaatt
     1921 gcttgagcgt ctggctgtcc ttgacgagat gcaggtccac attcgtcatc gcgacgtcat
     1981 tgaagccagt gagcttgtcc gctttcatcg cgacatcgat attgagatcg gaggcggtct
     2041 tcgccaacac atgggccgct gcccgcggct tggtcttgtg cggtgcggct tccgtgccga
     2101 cgatttcatc ggcatgcgac aacgtgagcg tcttcaaata cggccggccg tcgaaggatg
     2161 cggcgcggat gacgaccttc acagtgtcgc cggatttcga aagatcgaca tgcgcatcat
     2221 cgccgggtga gaggcgaacc tgt
//
LOCUS       DASWOG010000079         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_166745,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000079 DASWOG010000000
VERSION     DASWOG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166745"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGB93_01590"
     CDS             complement(<1..181)
                     /locus_tag="VGB93_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0144854.1"
                     /translation="MLSDAGKSIAVSSVPALLEHAASTFPDHNALDFFGRKLTYTELA
                     RQVDRAARGFQRLGVA"
     gene            complement(250..714)
                     /gene="ptsN"
                     /locus_tag="VGB93_01595"
     CDS             complement(250..714)
                     /gene="ptsN"
                     /locus_tag="VGB93_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY0144855.1"
                     /translation="MRLTDLIAHDAIIPSLRAGTKKLVLQELSEKAARISGLPPREIF
                     NALLQRERLGSTGTGNGVALPHAKLKKCQRIFGIFARLEKPIDFEAIDGEPVDLVLML
                     VAPEFAGADHLKALARVARVLRDVTIAAKLRAARDPAALYAVLTQEPTSNAA"
     gene            complement(832..1425)
                     /gene="raiA"
                     /locus_tag="VGB93_01600"
     CDS             complement(832..1425)
                     /gene="raiA"
                     /locus_tag="VGB93_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0144856.1"
                     /translation="MTLRVSGKNIDIGASLRTYVDARLDLVVLKYAAEPTGGHVTIER
                     EGSGFRTDCTLHLDTGITLQAEGEASDAYTSFNKAADRIENRLRRQKDRSKVRVANGA
                     GESAFTVPQLEPIGAAGEIDDLDGTNPAVIAESFTNIADMSVSAAVEKLDAGDSPVVF
                     FRHVTDRHANLVYRRADGNIGWIDLGKSDLGKSAKGS"
     gene            complement(1651..>1943)
                     /locus_tag="VGB93_01605"
     CDS             complement(1651..>1943)
                     /locus_tag="VGB93_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEY0144857.1"
                     /translation="SNKYVSTPRGLFELKYFFTASIASHSGGDAHSAEAVRFRIKQMI
                     EHESENDILSDDAIVEQLKKSNVEIARRTVAKYRESLRIPSSVERRRMRSLH"
BASE COUNT          410 a          546 c          578 g          409 t
ORIGIN      
        1 gcgcgacgcc gagacgctgg aaaccgcgcg cggcgcgatc gacttgacgc gcaagttccg
       61 tgtaggtcag cttacggccg aaaaaatcca gcgcgttgtg atcgggaaac gtgctggcgg
      121 cgtgctccag cagggccggg accgatgaaa cagcaatgga ctttccggca tcgctcaaca
      181 tcacggcaat ctagcgcatg ttgaggttgc gtccatccgg ctgtgatgcc ggtcccgaaa
      241 aatccggatt taggcggcgt tggacgtggg ttcctgggtc agcacggcat agagcgcggc
      301 gggatcgcgc gcggcgcgga gtttggccgc gatcgtgacg tcgcgcagga cgcgggcgac
      361 acgcgccagc gccttcagat gatctgcgcc ggcaaattcc ggcgccacaa gcatcaacac
      421 gagatcgact ggttcgccat caatcgcctc aaaatcgatt ggcttttcga gccgtgcgaa
      481 aatcccgaag atgcgctggc atttcttgag tttggcatgg ggcagcgcga cgccattgcc
      541 agtgccggtc gagccgaggc gttcacgttg caggagcgcg ttgaagattt cgcgcggcgg
      601 caggccggag atgcgagccg ccttttcgct caactcctgc agaacgagtt tttttgtccc
      661 ggcgcgtaac gaaggaatga tggcgtcgtg cgcaatcaga tcagtgagcc gcatgggaat
      721 attgacttcc aactttcgga caatcgaaga gagagccata tttgcaccgc gagaatatcg
      781 gcccgaaagc gcctgtgggc tgcccgtggg ctcgatattc gaaggttaga gttagctgcc
      841 ttttgcagat ttgccgagat cagacttgcc gagatcaatc cagccgatgt tgccgtcggc
      901 gcgacgataa acgaggtttg cgtggcggtc ggttacatgg cgaaagaaga cgaccggcga
      961 atcaccagca tcaagtttct ccacggcggc cgacacggac atgtctgcga tgttggtgaa
     1021 ggattcagca atgacggccg ggttggtacc gtccagatca tcaatttcac ccgccgcacc
     1081 gatcggctcc aattgtggca ccgtgaaagc cgactcgccc gcgccattcg cgacgcggac
     1141 tttgctgcga tccttttgcc ggcgcagacg gttctcgatg cgatccgcag ccttgttgaa
     1201 actcgtatag gcgtccgacg cctcgccttc ggcctgtaaa gtaatcccgg tgtcgagatg
     1261 taaagtacaa tcggtgcgaa agcccgagcc ttcacgttcg attgtcacat gaccgcccgt
     1321 cggttccgcc gcatatttca gaacgacaag gtcgagtcgc gcatcgacat aagtgcgaag
     1381 cgaagccccg atatcgatat tttttccaga gacgcgcaat gtcattggtt tacctccaat
     1441 ccactgtcgc tcctacacga tccgcccttc acggcggttg cttcagcgtt aggagcctcg
     1501 cgcggtgaag tcaatgggcg gcgccggcgc gtggcgcaaa gcgggaaaac tccgcccgaa
     1561 cgcaacggca acgctttgca gcaagtgcgc taatcgcgag gcgtttcaag cttttgtgga
     1621 aatgttcaaa gttcacgcga cgctcaaagt ttaatgtagg ctgcgcatac gccgacgctc
     1681 gaccgatgac gggatccgca aactttcacg gtattttgcc actgtgcggc gcgcaatttc
     1741 gacgttcgat tttttcaact gttcgacgat cgcatcgtcc gacagaatat cattttcgga
     1801 ttcgtgctcg atcatctgtt tgatacgaaa ccgcaccgct tccgccgagt gcgcgtcgcc
     1861 gccgctgtgc gatgcgatag aggcggtgaa gaaatatttc agttcaaaga gaccgcgcgg
     1921 cgtcgaaaca tatttgttcg acg
//
LOCUS       DASWOG010000080         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_93600,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000080 DASWOG010000000
VERSION     DASWOG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93600"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..542
                     /gene="rpsK"
                     /locus_tag="VGB93_01610"
     CDS             150..542
                     /gene="rpsK"
                     /locus_tag="VGB93_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY0144858.1"
                     /translation="MAKEATTRVRRRERKNIVSGVAHVNSSFNNTMITISDAQGNTIS
                     WSSAGTMGFKGSRKSTPYAAQMAAEDAARKAAEHGMRTLEVEVSGPGSGRESALRALQ
                     ASGFTVTSIRDVTPIPHNGCRPRKRRRV"
     gene            610..1626
                     /locus_tag="VGB93_01615"
     CDS             610..1626
                     /locus_tag="VGB93_01615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0144859.1"
                     /translation="MIQKNWQELIKPNKLEVSPGDDSKRLATVVAEPLERGFGVTLGN
                     SLRRILLSSLQGAAITSVHIDGVLHEFSSIPGVREDVTDIVLNIKDIAIKMQSEGPKR
                     MVLKKTGPGKVTAGDISTVGDVQVLNPNLVICTLDEGAEIRMEFTVNTGKGYVTADRN
                     RSEDAPIGLIPVDSLYSPVRKVSYRVENTREGQILDYDKLTMQLETDGSVSPEDAVAY
                     AARILQDQLNVFVNFEEPRREEVAPSIPELAFNPALLKKVDELELSVRSANCLKNDNI
                     IYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVTGWPPENIDELAKRF
                     EEHY"
     gene            1707..2123
                     /gene="rplQ"
                     /locus_tag="VGB93_01620"
     CDS             1707..2123
                     /gene="rplQ"
                     /locus_tag="VGB93_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0144860.1"
                     /translation="MYHGRAKRRFNRTAEHRKAMFANLSQALIKHEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRQAIAQIKDVDLVRKLFDVLGPRYKTRNGGYTRVLKAGF
                     RQGDNAALAVIEFVDRDEDAKGKDSGPVREEVAAGA"
     gene            2233..2334
                     /locus_tag="VGB93_01625"
     CDS             2233..2334
                     /locus_tag="VGB93_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HEY0144861.1"
                     /translation="MGQLGADRALGYGLKYATAFGDTHLDTLAAKSG"
     gene            2358..>3034
                     /locus_tag="VGB93_01630"
     CDS             2358..>3034
                     /locus_tag="VGB93_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0144862.1"
                     /translation="MKEISRFGIVVALTLGSALAVAPLAADPAVPTSAGEITLSFAPI
                     VKKVAPAVVNVYASRTDVQPRNPLFDDPVFRHFFGDSQEQRPGGPTAQSLGSGVLIDP
                     SGLVVTNYHVIAGMTDVKVALSDRREMPAKIVLRDQRTDLAVLRLDGKGPFPYMEFDD
                     SDKLDVGDLVLAIGDPFGVGQTVTQGIISALARTEEGISHYGFFIQTDAAINPGNSGG
                     ALVDMRGR"
BASE COUNT          612 a          884 c          933 g          605 t
ORIGIN      
        1 gcgaagccga tcgccggcaa gaagaagtaa tcgcgccgaa cccactcgcg ccctggctgc
       61 ttcatgtgcc ggggtgcatg tgattttgac gtttaaacca agaagcccga gcgcctggaa
      121 ttacgggcgc gctggaagga ccaagagcaa tggcaaaaga agcaaccaca cgcgtccgcc
      181 gccgcgagcg caagaatatc gtctccggcg tggcgcatgt gaattcgtcg ttcaataata
      241 caatgatcac catcagcgac gcgcagggca atacgatttc ctggtcgtcg gccggcacga
      301 tgggatttaa gggttcgcgt aaatcgacgc cttatgctgc gcagatggcg gctgaggatg
      361 cggcgcgcaa agccgccgaa cacggcatgc gcacgcttga agtcgaagtc tcgggcccgg
      421 gttcgggtcg cgagtcagca ctgcgcgcct tgcaggcctc gggcttcacc gtcacatcga
      481 tccgcgacgt gacgccgatc ccgcataacg gctgccgccc gcgcaagcgc cgccgcgtct
      541 aattgtttta actcgcagag gctttcggta ttggccggac gcccctcata cccccatgag
      601 gatgctctcg tgattcagaa gaactggcag gagctcatta agccgaacaa gctcgaggtt
      661 tccccgggcg atgattccaa gcgtctcgca accgtcgttg cggagccgtt ggagcgtggt
      721 ttcggtgtga cgctcggcaa ttcgctgcgg cggattcttt tgtcgtcgct gcaaggcgcg
      781 gcaattacct cggtacatat cgacggcgtg ctgcatgaat tctcgtcgat ccccggtgtt
      841 cgcgaggacg tgaccgatat cgtcctgaac atcaaggaca tcgcgatcaa gatgcagagc
      901 gagggcccga agcgcatggt cttgaaaaag accggcccgg gcaaagtgac cgccggtgac
      961 atttcgaccg ttggcgatgt ccaggtgctg aatcccaacc tcgtgatctg cacgctcgac
     1021 gagggcgcgg aaatccgcat ggaattcact gtcaacaccg gcaaaggcta cgtgacggcg
     1081 gatcgtaacc gcagcgaaga tgcgccgatc ggcttgatcc cggtcgatag cctttattcg
     1141 ccggtgcgca aggtcagcta tcgcgtcgag aacactcgcg agggtcagat cctcgattac
     1201 gacaagctca ccatgcagct cgaaaccgat ggctcggttt cgccggaaga tgccgtggct
     1261 tatgccgcgc gcattctaca ggatcagctc aatgtcttcg tcaatttcga agagccgcgc
     1321 cgcgaggaag tcgcgccgtc gatccccgaa ctcgctttca atccggcgct gctcaagaag
     1381 gtcgacgaac tcgaactgtc ggtgcgttcg gcgaactgcc tgaagaacga caacatcatc
     1441 tacatcggcg atctgatcca gaagacggaa gccgagatgt tgcgcacacc gaacttcggc
     1501 cggaagtcct tgaacgagat taaggaagtg ctggcgcaga tgggcctgca cctcggcatg
     1561 gaagtcactg gctggccgcc ggagaatatc gacgaactcg ccaagcggtt cgaagagcac
     1621 tattgagatc gctccttccc cgaaaacggg gaggggaata acacccggcc gtaccttggc
     1681 acggcggccg cataaggaga ctggccatgt atcacggtcg cgcgaagcgc cgcttcaatc
     1741 gtacagccga gcatcgcaag gcgatgttcg ccaatctttc gcaggcgctc atcaagcatg
     1801 agcagatcgt cacgaccctg ccgaaggcaa aagaccttcg tccggttgtc gagaagctcg
     1861 tcacgctcgg taagcgcggc gacctgcatg cgcgccgtca ggcgattgcg cagatcaagg
     1921 atgtcgatct cgttcgcaaa ctcttcgatg ttctcggtcc gcgctataag acccgcaatg
     1981 gcggctacac gcgcgtgctg aaagccgggt tccgccaggg cgacaatgcg gctcttgccg
     2041 tcatcgagtt cgtggatcgg gacgaggatg cgaagggcaa ggattcaggc ccggttcgcg
     2101 aagaggtcgc ggcgggggcc tgagcgccat gctggtgata tcggtgccat cgtcggcgtg
     2161 ccgaagttga ttttacattt ggaaggggcg gcgctggcac tcagcgccgc ctttttcttt
     2221 tggcgcggga agatgggcca actcggcgcc gatcgggcgc ttggctatgg gcttaaatat
     2281 gccacggcgt tcggcgacac acatttggat acattggccg ctaaatccgg gtgattggtg
     2341 gaattgacga ggaccgcatg aaagagattt cgcgcttcgg gattgttgtg gctttgactc
     2401 tcggctcggc tcttgccgta gcgcctctcg cggccgaccc ggccgtgccg acgtcggccg
     2461 gtgagatcac tttgtctttc gcgccgatcg tcaagaaggt cgcgcccgcc gtcgtcaacg
     2521 tttacgcgtc gcgtaccgac gttcagccgc ggaatccttt gttcgacgat ccggtcttcc
     2581 ggcatttctt tggtgattcg caggaacaaa gacccggagg cccgaccgcg caatcgcttg
     2641 gctccggcgt gctgatcgat ccgagcggcc tcgtcgtcac caattatcac gtgattgcag
     2701 gcatgaccga tgtgaaggtc gcgctctctg accgtcgcga aatgccggcg aagattgtgc
     2761 tgcgcgatca gcgcaccgat ctcgctgtgc tgcggctcga tggcaaagga ccgttcccgt
     2821 atatggagtt cgacgattcc gacaagctcg atgtcggcga tctcgtgctt gccatcggcg
     2881 accccttcgg tgtcgggcag accgtgacgc aaggtatcat ctcggcactg gcgcgtacgg
     2941 aagaaggtat ttcgcactac ggcttcttca tccagacgga tgcagcgatc aatcccggca
     3001 attctggcgg cgcgctggtc gatatgcgtg gtcg
//
LOCUS       DASWOG010000081         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_93612,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000081 DASWOG010000000
VERSION     DASWOG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93612"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VGB93_01635"
     CDS             complement(<1..1286)
                     /locus_tag="VGB93_01635"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001717 - conversion of seryl-tRNAsec to
                     selenocys-tRNAsec [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0144863.1"
                     /translation="MHDIKWIRENPEAFDAGLKRRGLEPQSAALLVLDDKRRAAIAKA
                     QTAQERRNAASKDVGAAMKAGDGTRAAVLKQEVADLKTALTTYENEERSAIATLDAAL
                     AQIPNIPSPDVPDGKDENDNPELRAVGSKPEFGFAPKEHFEIGENLGLMDFDTAAKLS
                     GARFVVLKGALARLERALGQFMLDLHTSVHGYTEINPPLLVRDEAMFGTAQLPKFRDD
                     QFPTFLEPIARLDGMLEYERRRGEERDAIEAEAEVRGEQFVEDFIRGAKGRGLDERYG
                     RNLAMRDARYDLYAEFDLKYWNHQLAKRVLWLIPTAEVTLTNIVRESILDEKELPLRV
                     TACTPSFRAEAGAAGRDTRGMIRQHQFTKVELVSVTTPEEAPSEHERMLASAEEVLKR
                     LGLAYRIVTLCTGDMGFAAQKTYDIEVWLPGQGRYR"
     gene            complement(1302..2087)
                     /gene="tatC"
                     /locus_tag="VGB93_01640"
     CDS             complement(1302..2087)
                     /gene="tatC"
                     /locus_tag="VGB93_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0144864.1"
                     /translation="MTDADIESTRAPLIEHLIELRARLIKALLAFVAMFVVCFFFAKN
                     IYNILLVPYEHAAGPSAKLIFTAPQEYFFTQIQVALFAAAFLACPVIFAQIYAFVAPG
                     LYRQERRAFVPYLFATPVFFLLGALMVYFVVMPNLLGFFLGMQQAAQPGRAQIELLPR
                     VSEYLALIMTLVFAFGVVFQLPVVLTLLGRVGIVNAPFLRRQRRYAAVLVFVAAAILT
                     PPDVFSMMALAVPGLLLYELSIIAVAYLEKQRAEAAAKSTTGT"
     gene            complement(2099..2485)
                     /locus_tag="VGB93_01645"
     CDS             complement(2099..2485)
                     /locus_tag="VGB93_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEY0144865.1"
                     /translation="MFEFDAGKLLIIAVAALIFIGPKDLPRVMRQVGQAVGKLRRMGA
                     DFQAQFMEAMNEAGAEDIKASVNTLGDSAKINLDFDPLNVAKGEATQMVRQIEAPIGE
                     PSVRGPVMMPEAAAEPESVAGRVVSD"
     gene            complement(2516..2746)
                     /locus_tag="VGB93_01650"
     CDS             complement(2516..2746)
                     /locus_tag="VGB93_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0144866.1"
                     /translation="MGSLSIWHLLILGAVVLLLFGGKGKISDIMGDFAKGIKSFKKGL
                     ADEDAPAAATPASLDHRQAVEANPVSQSQKVN"
     gene            complement(2798..>3143)
                     /locus_tag="VGB93_01655"
     CDS             complement(2798..>3143)
                     /locus_tag="VGB93_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY0144867.1"
                     /translation="MFVSQPEAARAAIAVRVAEIGGGINAPFALASLSTMTVLTGSIL
                     IALALTGGGLSLDSAWAAAHVDEDFQARQWGQDREATARRNLRFADMAAAEKFWLLLH
                     GPEKRLEEQYNP"
BASE COUNT          650 a          964 c          946 g          583 t
ORIGIN      
        1 tcgcgataac gcccctgccc cggtagccag acttcgatat cgtaggtctt ctgcgcggca
       61 aaacccatgt cgccggtaca aagtgtgacg atgcgataag ccaggccaag ccgcttcagc
      121 acttcctcgg cagaggccaa catgcgctcg tgctcgctgg gcgcttcttc cggagtggtg
      181 accgagacga gttcgacttt ggtaaattgg tgctggcgaa tcatgccacg cgtatcgcgc
      241 cccgccgcgc ccgcctcggc ccggaaagac ggcgtgcagg cagtaacgcg cagcggcagt
      301 tctttctcgt cgaggatcga ctcgcggaca atattggtca gtgtcacttc cgccgtcggg
      361 ataagccaga gcaccctttt ggcaagttga tgattccaat atttgagatc gaattcggcg
      421 tagagatcat agcgcgcgtc ccgcatcgcg agatttctgc cgtagcgctc gtcgagcccg
      481 cggcccttgg caccacggat gaaatcttcg acgaattgtt cgccacgcac ttccgcctcg
      541 gcctcgatcg cgtctctctc ttcgccgcgg cggcgctcgt attccagcat tccgtcgagc
      601 cgtgcaatcg gctcaaggaa tgtcgggaac tgatcgtcgc gaaacttcgg taattgcgcc
      661 gtaccgaaca tcgcttcgtc gcggacgaga agcggcggat tgatctctgt atacccgtgc
      721 acgctggtgt gcagatcaag catgaactgg ccgagcgctc gctcaagccg cgcgagcgca
      781 cctttcagaa cgacgaaacg cgcgccggac aatttcgccg ctgtgtcgaa atccatcagg
      841 ccgaggtttt cgccgatctc gaaatgctct ttcggcgcga agccgaattc cggcttgctg
      901 ccgacggcgc gaagttccgg gttgtcgttc tcgtccttgc cgtcgggaac atcgggcgag
      961 gggatgttgg gaatttgcgc aagcgcggca tcgagcgttg cgattgcgct gcgctcttcg
     1021 ttctcatagg tggtgagcgc cgttttaagg tcggccactt cctgcttcag aaccgccgcc
     1081 ctggtgccgt cgccggcctt cattgccgcg ccgacatcct tggaggcggc attacggcgc
     1141 tcctgagccg tctgagcctt ggcaatcgcg gcgcggcgct tgtcgtcgag caccagcaag
     1201 gccgccgatt gcggctccaa cccacgccgc ttcaacccgg catcgaaagc ctcgggattc
     1261 tcgcgaatcc atttgatgtc atgcatgggg cgtactctgg atcaggtgcc ggtcgtagat
     1321 ttagccgccg cttcggcgcg ctgcttctcg aggtaggcga ccgcgataat cgaaagctcg
     1381 taaaggagca ggccgggcac ggcaagggcc atcattgaaa agacatctgg tggcgtgagg
     1441 atcgccgcgg cgacgaaaac caacacggcg gcatagcggc gctggcgccg taaaaagggt
     1501 gcgttgacga tacccacacg accgagcagc gtgagcacga cgggcaattg gaaaacgacg
     1561 ccaaaggcga agacgagcgt catgatcagc gccagatatt cgctgacacg cggcaaaagc
     1621 tcgatctgcg cccggcccgg ctgcgccgcc tgctgcatgc caaggaaaaa gcccagcaaa
     1681 ttgggcatga cgacgaaata aaccatcaaa gcgccgagca ggaaaaagac cggagtcgcg
     1741 aaaagatagg gcacgaaggc gcgccgctcc tgccgataaa gccccggcgc cacgaaggcg
     1801 tagatctgag cgaagatcac cgggcacgcc agaaacgccg cggcaaacag ggcgacctga
     1861 atttgggtga agaaatactc ctgcggcgcg gtgaaaatca gcttggcgct cggccccgcg
     1921 gcatgctcgt agggaacgag caggatattg tagatgttct tggcgaagaa gaagcagacg
     1981 acgaacatgg cgacaaaggc gagaagcgcc ttgatgagac gcgcccgcaa ttcgatcaga
     2041 tgctcgatca gcggcgcgcg ggtcgactcg atgtcagcgt cggtcatggg ggattatatc
     2101 aatcgctgac gacgcgcccg gccacagatt caggttcggc ggctgcctcc ggcatcatca
     2161 caggcccgcg cacggaaggc tcaccgatgg gcgcctcgat ctggcgaacc atctgcgttg
     2221 cttcgccctt cgcgacgttc agggggtcaa agtcaagatt aatcttggcg ctatcgccca
     2281 acgtattgac gctcgccttg atgtcctcgg cgccggcctc gttcatcgcc tccatgaatt
     2341 gtgcctggaa gtccgcgccc atgcggcgca atttcccgac agcctgaccg acctgccgca
     2401 tcacgcgcgg cagatccttc ggaccgatga aaatcagcgc cgcaacggcg atgatgagca
     2461 gcttgccggc atcaaattca aacatcggga gatgaacgcc gttggcgccg aaggatcagt
     2521 tgaccttctg cgactgggaa accgggtttg cttcaacggc ctggcggtga tcgagcgacg
     2581 cgggcgtggc tgcagccggt gcgtcctcgt cggcaaggcc cttcttgaag gacttaatgc
     2641 ccttggcgaa atcacccatg atgtctgaaa ttttgccttt gccaccaaac agcagaagca
     2701 ccacagcacc aaggatcaac aaatgccaga tcgacaacga tcccatttcg gactcctttt
     2761 cagtctgccc ggaacctatg ctcccggctc gaggaactca aggattgtac tgctcttcca
     2821 ggcgtttttc cggcccatgg aggagcagcc agaatttttc cgcggcggcc atgtcggcaa
     2881 agcgtaggtt gcgccgggcc gtcgcctcgc gatcctgccc ccattggcgg gcctgaaagt
     2941 cttcatcgac atgcgcggcg gcccaagcgg aatcgaggct cagcccgccg ccggtgagtg
     3001 ccagcgcaat gaggatcgag ccggtcagaa ccgtcatcgt tgacagactc gcgagggcga
     3061 agggcgcatt tatccctccc ccaatctcgg ccacccgaac cgcaatcgcc gcgcgggcag
     3121 cctccggctg ggagacaaac ata
//
LOCUS       DASWOG010000082         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_295230,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000082 DASWOG010000000
VERSION     DASWOG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295230"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB93_01660"
     CDS             <1..552
                     /locus_tag="VGB93_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0144868.1"
                     /translation="GNSVRGHGIVMHAKNVHIRSDDHLTTVVGVDNVIVVTTQDAVLV
                     LDRAHGDKVKQLVDRLKAENRREALEHKRSYRPWGYYQSIDNGPRYQVKRIVVKPGAQ
                     LSLQKHFHRAEHWIVVRGTAEVTRNSEILQVHENESIFLPIGAVHRLANPGKIDLELI
                     EVQTGSYLGEDDIVRLEDVYNRV"
     gene            complement(537..947)
                     /locus_tag="VGB93_01665"
     CDS             complement(537..947)
                     /locus_tag="VGB93_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144869.1"
                     /translation="MRLAPWLAILLIAVLSLTPSEWRPYASHSILTERFVTYFLAALI
                     LAIRFPRPAAGIEIAAWLSIYAGTLEIIQLLIPGHFGRYGDFVMSSLGALCGVAFGLL
                     ATIWWEHWHVQPAEGVSEHFETPGSAEDVAHTRL"
     gene            1167..1859
                     /locus_tag="VGB93_01670"
     CDS             1167..1859
                     /locus_tag="VGB93_01670"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0144870.1"
                     /translation="MSKQPASLRFSSAFLGVCLLASPALAQTYNSPQTQQVASAAPTG
                     RYGGGFFEFVMSGGRAAAYNQPAPAYYASATPAEPQRTMDPMFMRQEVAYAGSERSGT
                     IVIDTAHKFLFLVEKDGKALRYGVGVGREGFAWSGTERISRKAEWPDWTPPVEMLKRR
                     PDLPMHMAGGIGNPLGARALYLGGSLYRIHGTNEPYSIGTSVSSGCIRMMNDDVIDLY
                     GRVGVGTKVIVM"
     gene            complement(1846..3237)
                     /locus_tag="VGB93_01675"
     CDS             complement(1846..3237)
                     /locus_tag="VGB93_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0144871.1"
                     /translation="MARVVIVSNRVPLPSKGAKAGGLTVALKDVFKQGALWLGWSGEI
                     ATETSTSAKILNTGKLDFATIDLSEDDYRQFYVGFANSVLWPLLHFQTGLLEFDREDF
                     AGYLNVNRTYAKALVELLQPDDLIWVHDYHLIPLAEALRALGVKNRIGFFLHVPFVPA
                     SVFAIVPPAEKLLRMMLTYDVVGFQARQHLHDFEDCLLSFLNIARQSGEPIWYEGRPV
                     IVDAIPIGIDSRGFARQAKKAEQSRVALRLFDSMEGHAFMIGVDRLDYSKGLPNRFEA
                     FGYLLDRYPEHRMHVTYLQIAARSREDVAEYQRLKRELDRLSGEINGRYADFDWQPLR
                     YMTRAVARASLAGFHRQARVGLVTPLRDGMNLVAKEYVAAQDPENPGVLVLSQFAGAA
                     EEMTEAIIINPYDPEEITEAMHEALVMPLDERKDRHEKLYEKICQTTAVSYCRDFLNA
                     LAGGTNGHSLTSQ"
     gene            complement(3222..>3903)
                     /locus_tag="VGB93_01680"
     CDS             complement(3222..>3903)
                     /locus_tag="VGB93_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0144872.1"
                     /translation="YQQLRSVGRLAARCALTPDAGPWEYRGLASIHTYSAAMCWAALH
                     RLALIAERVGESAEAQEWEALARKLRAEILDRATMPEGWISGVLDAPFADATCLLLAE
                     VGLLPADDPRVTKTLDVVGKRLVRDGFGMRYDEADDFGHPETAFLVCTFWYVDALALA
                     GRRDEAREIFNNVLAVRNGAGLLSEDVMAGTQTLWGNFPQAYSHVGLFHSAARLSRSW
                     EEALWRAS"
BASE COUNT          829 a         1156 c         1180 g          738 t
ORIGIN      
        1 ggcaattcgg tgcgtggcca tggcatcgtg atgcacgcca agaacgtgca tatccgttcc
       61 gacgatcatc tcacgacggt ggtcggcgtc gataatgtca tcgttgttac gacgcaggac
      121 gccgtgctgg tgctcgaccg cgcgcatggc gacaaggtca agcaactcgt cgataggctc
      181 aaagccgaga atcggcgcga agctttggag cataaacgct cctatcggcc gtggggctat
      241 tatcaatcga tcgacaatgg cccgcgttat caggtcaagc gcatcgtggt gaagcccggc
      301 gcgcaattgt cattgcagaa acattttcac cgcgccgaac attggatcgt cgtgcgcggc
      361 acggccgagg tcacgcgcaa cagcgaaatc cttcaggtgc atgaaaacga gtcgatcttc
      421 ctgccaatcg gggcggtcca ccggctcgcc aatccaggca agatcgatct cgaattgatc
      481 gaagtgcaga ccggctcata tctcggcgaa gacgatatcg tgcggcttga ggacgtctat
      541 aaccgcgtgt gagcgacgtc ctcggcactg ccgggtgtct cgaaatgttc agagacgccc
      601 tctgcgggtt gcacatgcca atgctcccac cagatcgtcg caagcaggcc gaaggcgacg
      661 ccgcatagcg cgccgagcga actcatgacg aaatcgccgt agcgtccgaa atggcccgga
      721 atcagaagct ggatgatttc aagagtacca gcatagatgc tgagccaggc cgcaatttcg
      781 atgcctgccg ctggccgcgg gaagcgaatt gcgaggatca gggccgccag aaaataagtg
      841 acaaaacgct cagtcaggat cgaatggctg gcatacggac gccactcgct gggggtcagc
      901 gagagtacag cgatcaacaa gatcgcgagc cagggggcaa gtcgcatgaa tcgcttcaca
      961 ccaagatctt accacccgag cgcccgcggg ccaatgaaga accgcgaatt tgctgaaaga
     1021 acccgccgcc aatttaacaa tgcttttacc ctgacggccg ggtgttaaca ttatcgattc
     1081 cctaaccacg ccgcgcggat ttgagccgta agtgcgcgtt tcgcattata acaaaggtat
     1141 cgtcagcttt ctggttgtgg atttcgatgt cgaagcagcc tgcgtccttg cggttttcgt
     1201 ccgcctttct tggcgtctgt ttgctggctt cgccagcgct cgcccaaact tacaacagcc
     1261 ctcagacaca acaggtcgca agcgccgcgc cgaccggacg ttatggtggc ggtttcttcg
     1321 aattcgtcat gtcaggcggc cgcgccgctg cctacaatca gccggctcca gcctattacg
     1381 ccagtgcgac acccgccgaa ccgcagcgca caatggaccc gatgttcatg cgtcaggagg
     1441 tggcctatgc cggttccgaa cgttccggaa cgatcgtgat cgacacggca cataagtttc
     1501 tctttctcgt cgagaaggac ggcaaggcgc tgcgctatgg cgtcggtgtc ggccgtgagg
     1561 gcttcgcctg gtcgggcact gagcgcattt cgcgcaaggc cgaatggccg gattggacgc
     1621 cgccggtgga aatgctgaag cggcgcccgg acctgccgat gcatatggcg ggcgggatcg
     1681 gcaatccgct cggcgcgcgc gctctatatc ttggcggctc gctttaccgc atccatggaa
     1741 cgaacgagcc ttactcgatt ggcacgagcg tttcctccgg ctgcattcgc atgatgaacg
     1801 acgatgtgat cgacctttat gggcgcgttg gcgtcggcac caaggtcatt gtgatgtgag
     1861 cgaatggccg ttggtgccgc cggccagcgc attgaggaaa tcacggcaat agctgacggc
     1921 tgttgtctgg cagattttct cgtaaagctt ctcgtgccgg tccttgcgtt catcgagtgg
     1981 catgacgagg gcttcatgca tggcctcggt gatttcttcc gggtcgtaag gattgatgat
     2041 gatcgcctcg gtcatttcct ccgcggcgcc agcaaattgc gaaagcacga gaacgccggg
     2101 attttcggga tcctgcgcgg caacatattc cttggcgacg agattcatgc cgtcgcgcag
     2161 cggcgtgacg aggccgaccc gcgcctggcg gtggaacccg gcgaggctcg cacgtgcaac
     2221 cgcgcgggtc atatagcgca acggctgcca atcgaaatcg gcataacggc cgttgatctc
     2281 accggacagg cggtcgagct cgcgcttcag gcgctgatac tcggcgacgt cttcgcgcga
     2341 acgggcggcg atctgaaggt aggtcacatg catccgatgc tcaggataac gatcgagcag
     2401 ataaccaaaa gcttcaaacc ggttcggcag tcccttggaa taatcaaggc ggtcgacgcc
     2461 gatcatgaaa gcgtggccct ccatactgtc gaagagccga agcgcgacgc ggctttgctc
     2521 cgcctttttt gcctggcgcg cgaagccgcg cgaatcgatc ccgatcggaa tcgcatcgac
     2581 gatgacggga cgcccctcat accagatcgg ttcgccggat tgccgggcga tgttgaggaa
     2641 gctcagcagg caatcctcga agtcgtgcaa atgctggcgc gcctggaagc cgacgacatc
     2701 ataggtcagc atcattcgca aaagcttttc agccggaggt acgatcgcga agacgctggc
     2761 cggcacgaac ggcacatgca ggaagaagcc gatacggttc ttgacgccga gcgcccgcaa
     2821 agcttcggcg agcgggatca gatgataatc gtgtacccag atgagatcgt ccggctgcag
     2881 caattcgacg agggctttgg cataagtccg gttgacgtta aggtagccgg cgaagtcctc
     2941 gcggtcgaat tcgagcaatc cagtctggaa atgcagcaaa ggccagagca ccgaattggc
     3001 gaaaccgacg taaaattgcc gatagtcgtc ctccgacaaa tcaattgtag cgaagtcgag
     3061 cttgccggtg ttgagaattt tcgcggaggt tgaagtttcg gtcgcaatct caccgctcca
     3121 gccaagccaa agcgcgcctt gcttgaaaac atctttcaac gcgaccgtca ggccgccagc
     3181 cttcgcgcct ttcgacggca agggcacgcg atttgagacg attacgacgc gcgccataaa
     3241 gcttcctccc agcttcgcga aagccgcgcc gccgaatgaa agagtcccac atgtgagtag
     3301 gcttgcggga aattccccca gagcgtctgc gttcccgcca tcacgtcctc ggagagaaga
     3361 ccggcgccgt tgcggacggc gagaacgttg ttgaagatct ctcgcgcctc atcgcgccgg
     3421 ccggcgagag ccagcgcatc cacgtaccag aaggtgcaga cgaggaatgc cgtctccgga
     3481 tggccgaagt cgtcagcttc atcgtagcgc atcccgaaac cgtcacgcac aagccgcttc
     3541 ccgacgacgt cgagtgtttt ggtgacccgt gggtcgtccg ccggcaaaag tccgacctcg
     3601 gcgagcagca ggcaggtggc gtcggcgaac ggcgcatcga gcacgccgga aatccagccc
     3661 tccggcatcg tcgcacgatc caaaatctcg gcgcgcagct tgcgcgcgag cgcttcccat
     3721 tcctgcgcct cggcggattc accgacgcgc tcggcgatga gcgcaaggcg gtgcagggcc
     3781 gcccagcaca tcgcagccga ataggtatgg atgctcgcca gtccgcgata ttcccatggc
     3841 ccggcatcgg gcgtgagcgc gcaacgcgcg gcgagccggc cgacgctgcg caattgctga
     3901 taa
//
LOCUS       DASWOG010000083         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_130279,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000083 DASWOG010000000
VERSION     DASWOG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130279"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGB93_01685"
     CDS             complement(<1..229)
                     /locus_tag="VGB93_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY0144873.1"
                     /translation="MAASPALWPYLPYSSWRDTVATLQLWTQIVGKVRLTLTPWVNHS
                     WQVPLYVTARGLATSPIPFNDELLEIEFDFIS"
     gene            complement(437..1276)
                     /locus_tag="VGB93_01690"
     CDS             complement(437..1276)
                     /locus_tag="VGB93_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0144874.1"
                     /translation="MRATRLGLWCLCFAIGIGGAFAQAAGLRVCADPNNLPFSNAKGE
                     GFENKIVDLVAKDMGATVSYTWWAQRRGYVRNTLKAGLCDLWPGVATAVDMLATSRPY
                     YRSGYVFVTRADRHLKIASFDDLQLRGLKIGVQMVGNDAMNTPPAHALARRGIINNVR
                     GYMLYGDYEKPNPPAAIVDAVAKGDIDVAVVWGPLAGYFATKEPKALTLTPVEPAMDG
                     AQWPMVFDISMGMRRHERPFEDKINEILERERPRIDAILAAYHVPRLDKPQQNFSASD
                     TGR"
     gene            complement(1273..1860)
                     /locus_tag="VGB93_01695"
     CDS             complement(1273..1860)
                     /locus_tag="VGB93_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0144875.1"
                     /translation="MSSHFRQLDLRGPASLLGLALALGAAVSANADPAQSAPPAASQP
                     AAQAAPDGGVGDVPVTGLFVNGQKEPPLGELGKQYEGNVQAIAIGATLYDWYNCSGCH
                     FHGAGGIGPAFDDSVWLYGGRIDQIYDSIYQGRPNGMPMWGKLLPSSAIWQLAAYVKS
                     LETEAAKKPIPTKPVPAAPGPASLETDPQEGAPNP"
     gene            complement(1835..>2137)
                     /locus_tag="VGB93_01700"
     CDS             complement(1835..>2137)
                     /locus_tag="VGB93_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0144876.1"
                     /translation="VFYGTMDGWFKAVDAKTGALKWQFKTGSGIIGQPVAYRGPDNRE
                     YVAILSGIGGWSGAIVPGDLDPRDPTGALGFVGAVADLKQRTAAGGTLYVFALPPT"
BASE COUNT          424 a          649 c          637 g          427 t
ORIGIN      
        1 ggctgataaa gtcgaattcg atctcgagga gttcatcgtt gaatggaatc ggcgatgtgg
       61 ctagcccgcg tgcagtgaca tacagcggga cctgccagct gtgattgacc cacggcgtga
      121 gcgtaaggcg aaccttgccc acgatctgcg tccaaagctg cagggttgcg accgtgtcgc
      181 gccaactcga atacggcaga tatggccaaa gggcaggcga ggcagccatt gcattcctcc
      241 acttcaggct tgagtgctgc gagtcccagt tggacggcag ttggacgcct gaaaatccta
      301 gtcgtccgat cctaaccttc tgaattcccg tttcaaaaga cgacttggac ggtttggacg
      361 gctttttgtg tctactgtct acattgcatg cttgcatctt aggctatcga cggcctgaac
      421 ctggggaata tccagttcag cgaccggtgt cgcttgcgga aaagttttgc tggggtttat
      481 ccaggcgcgg cacatggtat gcggcgagga tcgcgtcgat ccgcggccgc tcccgctcca
      541 aaatctcgtt gattttgtct tcgaacggcc gctcatgccg gcgcatgccc atggaaatgt
      601 cgaagaccat cggccattgt gcgccgtcca ttgcgggttc gaccggcgtc aacgtcagcg
      661 ccttcggctc cttcgtcgca aaatagccgg cgagcggccc ccagacgacg gcaacatcaa
      721 tgtccccctt ggcaacggca tcgacgatgg cggcgggtgg attgggcttt tcataatcgc
      781 cgtagagcat gtagccgcgc acattattga tgatgccgcg tcgcgccaat gcgtgtgctg
      841 ggggtgtgtt catcgcgtcg ttgccaacca tttgcacgcc gatcttcaaa ccgcgaagtt
      901 gcagatcatc gaaggaggcg atcttcagat gccgatcagc gcgcgtcacg aaaacatagc
      961 ccgagcgata atagggccgc gacgtcgcga gcatatcgac tgcggtggcg acgccgggcc
     1021 aaagatcgca tagaccagct ttcaacgtgt tgcgcacata gccgcggcgc tgcgcccacc
     1081 acgtgtagga aacagtcgcg cccatgtcct tggcgacgag atcgacaatc ttattctcaa
     1141 agccttcgcc cttggcattc gaaaacggca gattgttggg gtcggcgcaa acgcgcaacc
     1201 ccgccgcctg cgcaaatgcg ccgccgatgc cgatcgcgaa acaaagacac cataagccca
     1261 gacgagttgc tctcatggat tcggcgcgcc ttcctgcgga tccgtctcca gactcgccgg
     1321 gccgggcgca gcaggtaccg gttttgtcgg aattggtttc ttcgccgcct ccgtctccag
     1381 cgatttcaca taggccgcga gctgccagat cgctgaactc ggcagaagct tgccccacat
     1441 cggcatgcca tttgggcgtc cctgatagat cgaatcgtag atctgatcga tacggccgcc
     1501 gtagagccag accgaatcgt cgaaggccgg cccaatgccc ccagcgccgt ggaaatggca
     1561 tccggagcaa ttgtaccaat cgtacagcgt cgcgccgatg gcgatcgctt gcacattgcc
     1621 ctcatattgc ttgccaagtt cgcccagcgg gggttccttc tgcccgttga cgaagaggcc
     1681 ggtgaccggc acgtcgccca cgccgccatc cggcgccgcc tgtgccgctg gttgcgacgc
     1741 agctggcggt gcagattgag ctggatcggc gtttgcagaa acggcagctc caagtgcgag
     1801 cgccagtccg agcagggacg ccggtccgcg tagatcaagt tggcggaagt gcgaagacat
     1861 agagcgtccc tcctgctgcg gttcgctgct tcaaatcggc aacggcaccg acgaagccaa
     1921 gcgcgcccgt cgggtcgcgc ggatcgagat ctccgggaac gatggcaccg gaccagccgc
     1981 cgatgccgga cagaatggcg acgtattcac gattgtccgg accccggtag gcgacgggct
     2041 ggccgatgat tccggaccca gtcttgaact gccatttcag cgcgccagtc tttgcgtcga
     2101 ctgccttgaa ccagccgtcc atcgttccgt agaagac
//
LOCUS       DASWOG010000084         9442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_111977,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000084 DASWOG010000000
VERSION     DASWOG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111977"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..788)
                     /gene="rpe"
                     /locus_tag="VGB93_01705"
     CDS             complement(84..788)
                     /gene="rpe"
                     /locus_tag="VGB93_01705"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0144877.1"
                     /translation="MIDAGPQRPIIVAPSILAADFGHLADEIRAVDAAGADWIHIDVM
                     DGRFVPNITIGPIVVEAVRRNTQKPLNVHLMIVEPERYLAAFAKAGADHLLVHAETSA
                     TTHLHSVLSQIRDLGKKAGVVLNPATPIEMIEYILHLCDIVLVMTVEPGFGGQKFLPE
                     MLPKIHRLRRLCRSRGLDPIIEVDGGENRETAGQAIAAGANAIVAGSAIFGSHDYSAA
                     IAALRAAAPLAMRTIS"
     gene            complement(852..1394)
                     /locus_tag="VGB93_01710"
                     /pseudo
     CDS             complement(852..1394)
                     /locus_tag="VGB93_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
     gene            complement(1391..2230)
                     /locus_tag="VGB93_01715"
     CDS             complement(1391..2230)
                     /locus_tag="VGB93_01715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEY0144878.1"
                     /translation="MIERVPAAPKISLVLADVDGTLVTTDKVLTEAAKAAARDDLTHA
                     GIAFAVTSGRPPRGMAMLIAPLALRTPIAGFNGGVFVNPDLSVIESHTLDLAAAKEAL
                     TLILNQGLDAWVYTAEAWLIRDKAAPHVAREAWTVKFDPEVVVAFTEVHLAHAVKIVG
                     VSDDLDLVADCEKAAQKTLGAKASAARSQPYYLDVTHPQANKGTVVTTLSRLLNIPAA
                     QIATIGDMPNDVLMFRKSGFSIAMGNSSDEVKGQANAVTASNEDEGFAKAVQKFVLRS
                     ADT"
     gene            complement(2227..3102)
                     /locus_tag="VGB93_01720"
     CDS             complement(2227..3102)
                     /locus_tag="VGB93_01720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEY0144879.1"
                     /translation="MRQSSRVPSQKISTLVSAVDGTLVTDDKVLHASARNAVVELHAR
                     GIIFAIVSSRPTRGLRMLLDPLRLTTPVCGFNGGVIATPDLSVITEHLLPPDIARRAV
                     ELLDARNIQAWVFVGEDWLLRDPGGAYIGREEHTVGFKPTIVEDFGPHLDGAAKIVGV
                     SANFALLAQCERDMRIALAEQASVARSQAYYLDVTHPLANKGVALSELVRLLGVPLAE
                     IAVIGDGDNDVAMFEQSGLSIAMGNASAEVQRAADFVTGSNHENGFSAAVERFILGNA
                     RSNAEAVIVRAGGRV"
     gene            complement(3099..4610)
                     /gene="zwf"
                     /locus_tag="VGB93_01725"
     CDS             complement(3099..4610)
                     /gene="zwf"
                     /locus_tag="VGB93_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0144880.1"
                     /translation="MVIFGATGDLTKRLVIPALYNLVCTKVLPEQFALIGVARAEGTA
                     ESWRDELYEMLKSYVDNAATEFDVSHIDEGAWKRLAERMTYVRGDLTKPEMYDRLRGA
                     LDHAEKPHGTQGNAIFYLAVADRLFDAVVEQLGKAKLTDQDGDGKRPFWRRVVIEKPF
                     GHSLDSARDLNVRLQRTLREDQIFRIDHFLGKETVQSIMAFRFGNGLFEPIWNRDRID
                     HVQITAAETVGVEQRGEFYEATGALRDMVPNHLFTLLSMVAMEPPTGFGAAAILSKKA
                     ELLTAIPAVKPERVVRGQYGVGTVLGKPVRAYRQEVNVAPDSNVETYVAMQLEIDNWR
                     WAGVPFYLRTGKHMLQRNTEIAIRFKQAPSAAFQDTPVDSLRPNWLVLRIAPDAGISL
                     QFEVKRPGPVVDLAAVKMAFSYDDWFPKEPNVGYETLIYDIMTGDPTLFMRADMVEQT
                     WRIVQPVLEAWAGDRAADLPVYAAGSEGPVEANTLLARDARSWRSVDAAGKSS"
     gene            complement(4708..5745)
                     /gene="gnd"
                     /locus_tag="VGB93_01730"
     CDS             complement(4708..5745)
                     /gene="gnd"
                     /locus_tag="VGB93_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY0144881.1"
                     /translation="MQIGMIGLGKMGGNMSRRLMKAGHRCVVFARSAKAREALAKEGA
                     TAAASLADMVKTLAPKPRAVWLMLPAGGTTDETVESLSSLLEPGDIIIDGGNSFYKDD
                     IRHAKTLAEKGIRYVDCGTSGGVWGLERGYCMMIGGPKEVVDYLDPIFAALAPGLGDI
                     PRTPDRANGDSRAEHGYIHAGPTGAGHFVKMVHNGIEYGLMQAYAEGFDILRNKDSKD
                     LPEDARFTLNLPDIAEVWRRGSVVSSWLLDLSAAALARDPQLKEFSGYVQDSGEGRWT
                     VEAAIEEAVSANVLSTALFARFRSRQEHTFGEKMLSAMRFGFGGHVEGQEPIDPKPKS
                     TGRSTAQQAAE"
     gene            complement(5778..8606)
                     /locus_tag="VGB93_01735"
     CDS             complement(5778..8606)
                     /locus_tag="VGB93_01735"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY0144882.1"
                     /translation="MNPLQQLETCGQSPWLDYLKRSLIESGELRRLIEHDGLKGVTSN
                     PSIFEKAIGESDEYSEALKQFQAQADHGISEIYEHLAIADIRAAADILLPVYQQTLGR
                     DGNISLECSPYLANDTEATVSEALRLWAAVARPNLMVKVPATSAGVPAIRQLIGRGLN
                     INITLLFSVEVYAQVVEAYMSGLEDFARSGGDVAKIGSVASIFVSRIDAAIDKRLDQL
                     ADPRLADRLRGKAAIANAKLAYRRYQELFSGPRWQHLAAAGAKTQRLLWASTSTKNPD
                     FKDTMYIEALIGSDTVDTIPPATMDAFRDHGQVAADAVEQDVDDARATLAELEQHGIS
                     LKEITDELLADGVQQFADAFDKLLGAVAGRRRVLLEGKHARQKIALGAAEMQGAFDQE
                     MDVWREQGLIRRLWSADKSLWSATDEDKWGGWLCIAQHEVADIDQLENFAQEVKQRGF
                     TDVVLLGMGGSSLGPEVLAETFGQQFGCPCFHMLDATDPAQINAIEQAVDLGKTLFIV
                     SSKSGTTLEPNIFMDYFLERVGAVRGADKAGGHFIAVTDPGSPLERRAQTLGFAWIFH
                     GVLSIGGRYSVLSKFGLVPAAAMGLDVKRFLDAVRPMQRACGPDVPPAENPGVQLGIA
                     LGVAATRFGRDKVTIIASPGIAGLGAWLEQLLAESTGKHGKGLIPLAGEPLTTPSRYG
                     GDRFFAYLELDGQIDPSQSAAVAALERAGHPVARINVKDIWHIGQEFFRWEIAVAVAG
                     AIIGIDPFDQPDVEASKDRTRALTEAYEKSQSLPDEEALFRENGLALYADPRNAAALG
                     RHNTLTGYLKGHFTQVHTGDYVALLAYIERNESHMRTLTSMRTRIRDRTRAATCLGFG
                     PRFQHSTGQAYKGGPNSGVFLQITCDDPVDIDVPGHSYSFGVVKAAQAGGDLAVLVDR
                     GRRALRVHIKNVDAGLAKLAQAMDEALE"
     gene            complement(8612..>9442)
                     /locus_tag="VGB93_01740"
     CDS             complement(8612..>9442)
                     /locus_tag="VGB93_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0144883.1"
                     /translation="AGDLEAGTYGGRNLHFGIREHAMGAILSGLTLSGIRAYGATFLI
                     FSDYMKPSIRLSALMELPVVYIFTHDSIGLGQDGPTHQPVEQLVALRAIPGLVTLRPA
                     DANEVVEAWRVIIALKQPACLVLSRQPLPTFDRARYASAAGVARGAYVMADAQNGKPD
                     VILIGTGSELALCIEAYEALKREDIPARVVSMPSWELFEQQDQVYRDSVLPPGITARV
                     SVEEGAVIGWDRYAGVGGARIGMQTFGASGPIKDLMTKFGFTPEKVLAAAKQQRTLVR
                     "
BASE COUNT         1810 a         2977 c         2797 g         1858 t
ORIGIN      
        1 gaggtcgacg atccaatgcg cgacccgctg cgccaaggcc tccggatcag cgaggatttc
       61 cagtttcgca ccgaatgcat tcgtcatgag atggttctca tcgccagcgg cgcggccgcg
      121 cgaagtgcgg caatcgccgc agaatagtca tgcgatccaa agatcgcgga cccagcaacg
      181 atggcgttcg cgcctgccgc gatcgcctgc cccgcggttt cccgattctc tccgccgtcg
      241 acttcaatga tgggatcgag gccgcgcgag cggcaaaggc gacgcaaacg gtggatcttc
      301 ggcaacatct ccggcaggaa tttctgcccg ccgaagccag gctcgacggt catcacgaga
      361 acgatatcgc aaaggtggag gatatattcg atcatctcaa tcggggtcgc cggattgagc
      421 accacgccag ccttcttgcc gaggtcgcgg atctgcgaaa gaacactatg gagatgggtc
      481 gtcgcagacg tctccgcatg gacgaggaga tgatcggcgc cagccttggc aaaagcagcg
      541 agatagcgct ccggctcgac gatcatcaaa tgcacattga gcggcttttg cgtattgcgt
      601 cgtactgctt caacaacgat cggcccaatt gtgatgttcg gcacgaagcg gccgtccatg
      661 acatcgatat ggatccagtc ggcgcccgcc gcatcgacgg cgcgaatttc gtcggcgaga
      721 tgcccgaaat ccgctgccaa gatcgacggc gcgacgatga tcggtctctg cggtccggcg
      781 tcgatcacag ataccgcacg cgatggcgct tcgcgacgtc ggcgatcttg ccgcttctct
      841 atctgctcca cgatctcgtc gacgatctcg tttggctgcg ggccgacatc gacggaaatc
      901 gcatgttcgt ccgctccggg ttcctccagt gtcgcaaact ggctgtcgag tagagccact
      961 ggcatgaaat gttcgtgccg cgccgtcatc cttcgtcgga tgaggtcgta ggatcctttg
     1021 aggtagatta acacgacccc cggacggttg ccgacgatga tgtcccgata ggagcgcttc
     1081 agcgccgaac aggtaagcac gccggattcg ccggccgcgc gccagtcatc gattttttcg
     1141 gctatcttcc gcagccaggg cagtcggtca gcatcggtca gcggcacgcc gctgtgcatt
     1201 ttctccacgt cctcgactgg atgcagatcg tcaccctcct gcaatcggct gccgagtgcg
     1261 gctgccagca acgccgcgac agtggatttt ccggagcccg agacacccat gacaatgacg
     1321 atcaccggtt caactatttg gtgcgcgatg ggctgcgccc gcaggcgctg gggttcatcg
     1381 tcgtgtcgca tcatgtatcg gccgaacgga gcacgaattt ctgcaccgcc ttggcgaagc
     1441 cttcgtcctc gttgcttgcg gtcactgcgt tcgcctgtcc cttcacctcg tcgctggaat
     1501 tgcccatggc aattgaaaag ccgctcttgc ggaacatgag gacatcattc ggcatatccc
     1561 cgatcgtggc gatctgcgcg gctggaatat tcagcagcct ggacagagtg gtcacaactg
     1621 ttcccttgtt agcttgcggg tgggtcacat cgagataata aggctgcgat cgcgccgcac
     1681 ttgccttggc acctagtgtt ttctgcgctg ccttctcgca gtccgcgacc agatcgagat
     1741 cgtcggacac gccgacgatt tttaccgcat gtgccaaatg cacctccgta aacgccacca
     1801 ccacctcggg gtcgaatttg accgtccacg cctcacgcgc cacatgcggc gcggccttgt
     1861 cgcggatcaa ccatgcctcc gccgtgtaga cccaagcgtc gagtccctga ttgagaatga
     1921 gggttagggc ctctttggcg gccgcgaggt cgagcgtgtg gctttcgatc acggagaggt
     1981 cgggattgac gaagactccg ccgttgaaac cggcgatcgg cgtccgcagc gccaacgggg
     2041 cgatcaacat cgccatgccc cgtggcggtc gcccgctggt gactgcgaag gcaatgccgg
     2101 catgagtcag atcgtcccgg gccgctgctt ttgccgcctc ggtcaacacc ttatccgtgg
     2161 tcaccagcgt gccgtctacg tcggccagga caagcgatat tttcggtgcg gcggggacgc
     2221 gctctatcat acgcggcctc cggctcggac aatcacggcc tcagcatttg agcgagcgtt
     2281 gccgagaatg aaccgttcga cggcggcgga gaagccgttt tcgtgattgc tgccggtcac
     2341 gaaatccgcg gcccgctgca cctcggcact ggcattgccc atggcaatgc tgaggccgct
     2401 ctgctcgaac atcgcgacgt cattgtcgcc gtcgccgatg acggcgatct ctgccagagg
     2461 cacgcccaac agcctcacca gttcagataa tgcgacgcct ttgttggcga gcggatgggt
     2521 gacatcgagg tagtaggctt gcgaacgcgc gaccgacgct tgctccgcga gcgcaatacg
     2581 catgtcgcgc tcacactgtg cgagaagcgc gaaatttgcg ctgacaccga cgatcttggc
     2641 tgcgccgtcg aggtgcggtc cgaaatcttc gacgattgtc ggcttgaaac ccaccgtgtg
     2701 ctcctcgcgc ccgatataag ccccgccggg atcgcgcagc agccagtcct ctccaacgaa
     2761 aacccaggcc tgtatattgc gtgcgtcgag cagttcgacg gcacgccgag caatgtcagg
     2821 cggcaagaga tgttcggtaa tcaccgaaag atctggtgtt gcgatcaccc cgccattgaa
     2881 gccgcacacg ggtgtcgtga gtcgcagagg atcgagcagc atccgcaagc cgcgggttgg
     2941 ccggctgctg acaatagcaa aaatgatccc acgcgcgtgc aactcgacca ccgcattccg
     3001 ggcagacgcg tgcaaaacct tgtcgtcggt caccaacgtg ccgtcaacgg ccgagacaag
     3061 agttgaaatt ttctgtgagg gaactcgaga ggattgcctc atgacgattt gccggcagcg
     3121 tcgacggaac gccaagaccg ggcatcacga gccaaaagcg tattcgcttc gaccggtcct
     3181 tcacttcccg cggcgtagac cggcagatct gccgcgcggt cgcccgccca agcctcgagt
     3241 accggctgca cgatccgcca ggtctgctcc accatgtcgg cgcgcatgaa taatgtcgga
     3301 tcaccggtca tgatgtcata gatgagcgtt tcgtagccga cattgggctc cttcggaaac
     3361 cagtcatcgt agctgaacgc catcttcaca gcggcaagat ccaccactgg cccggggcgc
     3421 ttgacttcga attgcaagga gatccccgcg tccggcgcga tgcgcaacac caaccagttg
     3481 gggcgcaagc tgtcgacggg cgtgtcctgg aacgcggcgg agggcgcctg cttgaaacgg
     3541 atcgcgattt ccgtattccg ttgcaacatg tgcttgccgg tacggaggta aaatggaaca
     3601 ccggcccaac gccaattgtc gatctcaagc tgcatcgcga cataggtctc gacgtttgag
     3661 tccggcgcga cattgacttc ctgccggtag gcccgcaccg gcttacccag cactgtgccg
     3721 acgccatatt ggccgcgcac cacgcgttcc ggcttgacgg cgggtatagc agtcaacaat
     3781 tcggctttct tggaaaggat cgccgcggca ccgaagccgg tcggcggctc catggccacc
     3841 atcgaaagca atgtgaacaa gtgattgggc accatatcgc gtaatgcgcc cgtcgcttcg
     3901 tagaactcgc cgcgctgctc gacgccgacc gtctccgccg ctgtaatctg cacgtgatcg
     3961 atccggtcgc ggttccagat cggttcgaac aacccattgc cgaaccggaa cgccatgatg
     4021 ctctggactg tttcctttcc aagaaagtgg tcgatgcgga agatttggtc ctcccgcagc
     4081 gtgcgttgga ggcggacatt caaatcgcgc gccgagtcca agctatggcc aaatggcttc
     4141 tcgatcacca cgcggcgcca aaacggacgc tttccatccc catcctggtc ggtgagcttc
     4201 gcctttccga gttgctccac gacggcatcg aatagccgat cggcgaccgc aagatagaag
     4261 atggcgttac cttgagtccc gtgaggtttt tccgcatggt cgagagcgcc gcgaagcctg
     4321 tcgtacattt cgggcttcgt caggtcgccg cggacatatg tcatccgttc cgcgagccgc
     4381 ttccaggcgc cctcgtcaat atgggagaca tcaaattccg tggccgcgtt atcgacatag
     4441 cttttcagca tctcgtaaag ctcgtcacgc cagctttccg cagttccctc tgccctggcc
     4501 acgccgatca gcgcgaattg ctcaggcagc acctttgtgc atacgaggtt atagagagcc
     4561 ggaatcacca gacgcttggt caggtcgccg gtggcgccga agatcaccat cgcgcatggg
     4621 tcggctggct tggcctggcg ccgcggcggg ctctggatca cccgtgcggt cttcggcgca
     4681 tggactgtgg gatcgtctaa catgggccta ctcggcggct tgctgtgcag tcgatcggcc
     4741 cgttgacttc ggctttgggt caataggttc ttgcccttca acatgccctc cgaaaccgaa
     4801 gcgcatggcc gaaagcatct tctctccgaa ggtgtgctct tgccgcgagc ggaagcgggc
     4861 aaagagcgcc gtcgaaagca catttgccga taccgcttcc tcgatcgcgg cttcgacggt
     4921 ccagcggcct tcgccggaat cctgaacgta gccggagaat tccttgagct gcggatctct
     4981 ggccaacgct gccgcactga gatcgagcaa ccaagaggag acgacgctgc cacgccgcca
     5041 aacttcagcg atatccggca ggttcagcgt gaaacgtgcg tcctcgggca aatccttcga
     5101 atctttgttg cgcagaatgt cgaagccttc cgcataggcc tgcatcaatc cgtattcgat
     5161 gccgttatgc accatcttga caaagtgccc ggcgccagtc gggccggcgt gaatgtagcc
     5221 gtgctcggcg cgggaatcgc cattcgcacg gtccggtgtg cgcgggatgt cgcccagtcc
     5281 gggcgccaac gcagcgaaga tcggatcgag ataatcgacg acttcttttg ggccgccgat
     5341 catcatgcaa tagccgcgtt cgagtcccca gacgccgccg gacgtgccgc aatcgacata
     5401 gcgaatgccc ttctcggcta atgtcttcgc gtggcgaata tcgtccttgt agaaggaatt
     5461 gccgccgtcg atgatgatgt caccgggctc cagcaggctg ctcagactct cgacggtttc
     5521 atcggtggtt ccgccggcgg gcaacatcaa ccacacagcg cgcggctttg gcgcgagagt
     5581 cttcaccata tcggccagcg acgcagcggc tgtggcgccc tctttcgcca acgcttcgcg
     5641 ggccttggca ctgcgcgcga atacaacgca gcgatgcccc gctttcatca ggcggcggga
     5701 catgttgcca cccattttgc caagtccaat cataccgatc tgcatcgcag cactccttgt
     5761 aaatgacgaa agcgccgcta ttcgagcgct tcatccatgg cctgcgctag ctttgccagc
     5821 cccgcgtcga cattcttgat gtggacacgc aacgcacggc gaccgcggtc cacaagcacg
     5881 gcgagatcgc caccggcctg cgcggccttc accacgccga agctgtagga atggcccggc
     5941 acatcgatgt cgactggatc gtcgcaggtg atctgcagga acacgccgga attcggcccg
     6001 cctttgtagg cttgcccggt cgaatgctgg aaacgtggcc cgaatccgag acaggtggcg
     6061 gcgcgggtgc ggtcacggat gcgcgtgcgc atagaggtca acgtccgcat gtgtgactcg
     6121 ttgcgctcga tgtaagcgag cagcgccaca taatcgccgg tatgcacctg cgtgaaatgt
     6181 cccttcagat agccggtcag cgtattgtgc cggccgagcg cggctgcgtt gcgcggatcg
     6241 gcgtagaggg ccaagccatt ctcgcggaac agcgcttcct cgtccggcag actctgcgat
     6301 ttctcatatg cctcggtgag cgcgcgcgtt ctgtccttgc ttgcctcgac atccggctgg
     6361 tcgaatgggt cgataccgat gatcgcaccg gctacggcta cggcgatctc ccagcggaag
     6421 aactcctggc cgatatgcca gatatccttc acgtttatcc gcgcgacggg atggcctgcc
     6481 cgctccagcg ccgccacggc cgcgctctgc gatggatcga tctgtccatc cagctccaga
     6541 taagcgaaga accgatcgcc gccgtagcga ctcggcgtgg tgagcggttc ccccgcgagt
     6601 ggaatcagac ctttcccatg cttgcccgtg ctctcagcta gaagctgctc aagccaggcg
     6661 ccgaggccgg cgattcctgg cgaggcgatg atcgtcacct tgtcacggcc aaagcgtgtc
     6721 gcagcaacgc ccagggcgat accaagctgc acgcccggat tttccgccgg tggtacatcg
     6781 gggccgcagg cgcgctgcat cggccgcacc gcatcgagga agcgtttgac gtcgagaccc
     6841 atcgccgcgg ccggcaccag gccaaatttc gacaatacgg aatagcgccc accgatcgac
     6901 agcacgccgt gaaatatcca ggcgaagccc aacgtctggg cgcgccgctc cagtggcgag
     6961 ccgggatcag tcaccgcgat aaagtggcca ccagccttat ccgcgccacg caccgcaccc
     7021 acacgctcaa ggaaatagtc catgaagatg ttgggctcca gcgtggtgcc ggattttgag
     7081 gagacgataa acagtgtctt gccaagatct accgcctgtt cgatggcgtt gatctgtgcg
     7141 ggatcggtag cgtccagcat atggaagcac gggcagccga actgctgccc gaaggtctcc
     7201 gccaaaactt caggaccaag gctcgatcct cccatcccca gcagaacgac gtcggtaaag
     7261 ccgcgctgct tgacctcctg tgcaaagttc tcgagctggt caatatcggc cacctcgtgc
     7321 tgcgcaatgc agagccagcc accccatttg tcttcgtccg ttgcggacca gagcgacttg
     7381 tcggcggacc aaagccggcg tatgagcccc tgctcgcgcc agacgtccat ttcctgatca
     7441 aaggcgccct gcatctccgc cgcgcccagc gcaatctttt gtcgggcgtg ctttccctcg
     7501 agcaagacac ggcgacgccc tgcgacggca cccagcagtt tgtcgaatgc atcggcgaat
     7561 tgctgcacgc catccgcgag caattcgtcc gtgatctctt tcagcgagat gccatgctgt
     7621 tccagctcgg cgagcgtcgc gcgagcatcg tctacgtcct gctctacggc gtccgctgcc
     7681 acctgaccat ggtcgcggaa agcatccatg gtcgccggcg ggatcgtgtc cacagtgtcg
     7741 ctgccgatga gcgcctcgat atacatcgtg tccttaaaat ctgggttttt ggtgctggtc
     7801 gaggcccaga gcaggcgctg cgtttttgcg cccgctgcgg ccagatgctg ccagcgtggg
     7861 ccggaaaaca actcttgata acgacggtag gcaagtttcg cattggcgat cgcggccttg
     7921 ccgcgcaacc gatccgccag tcgtggatca gcaagttggt cgagcctctt gtctatcgcc
     7981 gcgtcgatgc ggctgacgaa gatgctggca acgctgccga tcttggcgac atcgccgccg
     8041 gaccgcgcga agtcttcaag cccggacata taggcttcga ccacttgcgc gtagacctcg
     8101 actgagaaga gcaaggtaat gttgatgtta agcccacgac caatcagctg gcggatcgcc
     8161 ggaacaccgg ccgaggtcgc cgggaccttc accatcaaat tgggacgcgc caccgccgcc
     8221 cacagccgca gcgcctcgct cactgtcgcc tcggtatcat ttgccagata gggcgagcat
     8281 tccagactga tattgccgtc ccggcccagc gtctgttggt agaccggaag caggatatcg
     8341 gcagcagcac ggatatcggc gatcgccaga tgctcgtaga tttcggaaat gccgtggtct
     8401 gcctgcgctt gaaactgctt caacgcttct gaatattcgt cgctctcgcc gatcgccttt
     8461 tcgaagattg agggattcga ggtgacgccc ttcagcccgt catgctcgat gagcctgcgc
     8521 aattcgccgc tctcgatcag gctgcgcttt agataatcga gccagggcga ctggccgcat
     8581 gtttcgagct gctgcagcgg attcatgcct tctacctaac cagagtgcgc tgttgtttcg
     8641 ccgcggcgag caccttctcc ggcgtgaagc cgaacttcgt cataagatcc ttgatcggcc
     8701 ccgacgcgcc gaatgtctgc atacctatcc tggcgccgcc gacgccggcg taacgatccc
     8761 agccgatgac cgcaccttcc tccaccgaga cccgtgcggt gatgccgggt ggcagcacgc
     8821 tgtcgcggta gacctgatcc tgttgttcaa acaattccca ggacggcatg ctgacgacgc
     8881 gcgccggaat gtcctcccgc ttgagcgctt cgtaagcctc gatgcagagc gcaagctcgc
     8941 tgccggtgcc gatgagaata acgtccggct taccgttttg ggcgtcggcc atcacatagg
     9001 cgccgcgcgc gacgccagcg gcggaagcgt agcgtgcgcg gtcgaacgtc ggtagcggct
     9061 ggcggctcag aacgaggcag gccggctgct tcagcgcgat gatgacgcgc caggcctcga
     9121 cgacttcatt ggcatcggcc ggccgcagcg tgacaaggcc ggggatcgcg cgtagcgcga
     9181 caagctgctc gaccggttgg tgtgtcggac cgtcctgccc gaggccgatt gaatcatgtg
     9241 tgaagatgta gacgaccgga agctccatca gcgcactcag gcggatggac ggcttcatat
     9301 aatcggagaa aatgaggaaa gtcgccccgt aagcgcgaat tccggacaat gtgagcccac
     9361 tcagaatcgc gcccatggcg tgttcgcgaa tcccgaagtg cagattgcgg ccaccgtatg
     9421 tgccggcctc aagatcgccg gc
//
LOCUS       DASWOG010000085         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_203511,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000085 DASWOG010000000
VERSION     DASWOG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203511"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..994
                     /locus_tag="VGB93_01745"
     CDS             221..994
                     /locus_tag="VGB93_01745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0144884.1"
                     /translation="MPEARKNAPRTSDLAFIEVSHAGETFRIALKRISTARRFTLRVR
                     AATRDVVLTMPPRGSVIGARNFVERHAAWIGVRLQRLPAPMPFGAGEIVPVRGINHRI
                     VERPHQRGTAWIEAAADPQSDKSLPMLCVAGERAHVARRVQDFLMREARRDIETAVAR
                     HAGKLGVSPRRITLRDTTSRWGSCSSTGALNFSWRLIMAPSYVLDYLAAHEVAHLVHM
                     NHSPAFWKILGKLSAHVERAEAWLKINGAGLLRFGPFKA"
     gene            complement(1047..1736)
                     /locus_tag="VGB93_01750"
     CDS             complement(1047..1736)
                     /locus_tag="VGB93_01750"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY0144885.1"
                     /translation="MIDEHPLATRLYGRRKGKKLSALQVNLRETLLPRLALDPAQPII
                     SAPSLFPNAPRDIWLEIGFGGGEHLAAEAAAHPDEGFIGCEFFENGVVKVLTLIAHKD
                     LDNVRLYQGDAGAIIEALPPQSLAGAYLLYPDPWPKRRQRKRRFLSDTMLKRLARIMR
                     PGAEIRFATDIDDNAGWTLARVLESPDFEWGAQSVCDWREPWSGWASTRYEAKALAAE
                     RTPVYLTFVRT"
     gene            complement(1770..>2894)
                     /gene="metK"
                     /locus_tag="VGB93_01755"
     CDS             complement(1770..>2894)
                     /gene="metK"
                     /locus_tag="VGB93_01755"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0144886.1"
                     /translation="ISDEIVDLHFRESAKAGIDPYEVRVACETLATTNRVVIAGETRG
                     PKLSSDVIEHTARKAIKAIGYEQDGFHWEHCKFEVLLHAQSVDIAQGVDAAGNKDEGA
                     GDQGIMFGFACRETPELMPAPLYYSHKILEVLAKARHAGEGDAAKLGPDAKSQVTIQY
                     ADGKPVAATQIVLSTQHLDPNLSSDDVRAIVEPYIRRTLPAQWINEQTIWHVNPTGKF
                     VIGGPDSDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAVRYLAKNIVAA
                     DLADRVTIQLAYAIGYSEPLSIYIDTHGTGRVAEDKLEAVLPQVMRLSPRGIRDHLQL
                     NKPIYARTSAYGHFGRAPEADGGFSWEKTDLADKLRASFS"
BASE COUNT          526 a          854 c          929 g          585 t
ORIGIN      
        1 cgcgctaccg gcagttcatc gcgcccaaaa tccgcgagtt ccatgccgcg gtcgcccccg
       61 tctaagctct tgcaatcacg gtaaattggc ctgtggcggc gtggctgccc tctgtgttgc
      121 gccgcgaaaa gactgtgccc cgagcggcca aggtaaaatg ccgccaatgt gagccgctga
      181 ttcagtagcg actcaggcgg gctgcggcta aattcagtcc atgccggaag ctcgcaagaa
      241 cgcaccgaga acatctgacc tcgcctttat cgaggtttcg cacgccggcg aaacgtttcg
      301 aattgcgttg aagcgcatca gcaccgctcg acgtttcaca ctgcgcgtgc gcgccgcgac
      361 gcgcgatgtg gtgcttacga tgccgccgcg cggttcggtc attggcgcgc gcaatttcgt
      421 cgagcggcac gctgcgtgga tcggtgtgcg cttgcaacga ttgcctgcgc cgatgccgtt
      481 cggcgccggc gagatcgtgc ctgtccgagg aatcaatcat cgcatcgtcg aacgtccgca
      541 tcagcggggc accgcatgga tcgaggcggc ggcagatccg caaagcgaca agagtctgcc
      601 tatgctgtgc gtggcgggcg agcgggcgca tgtcgcgcgg cgcgtgcagg attttctgat
      661 gcgcgaggcc agacgcgaca tcgagactgc cgttgcgcgc cacgcaggta agctcggagt
      721 cagcccgcgc aggatcactc tgcgcgacac gacgagccgc tggggttcgt gctcatcgac
      781 tggtgcgctt aatttctcgt ggcggctgat tatggcgccg agctatgtgc tcgactatct
      841 cgcggcccat gaagtcgcgc atctcgttca catgaaccat tcgccggcgt tctggaaaat
      901 cctcggcaag ctttccgcgc atgtcgagcg cgccgaagcc tggctgaaga tcaatggcgc
      961 gggcctcttg cgcttcgggc ctttcaaagc ctgattttaa cgcgttttct tcacgcgaac
     1021 cggtttccat ttcgctcgaa aacgctctat gtccgcacga acgtgagata gaccggcgtg
     1081 cgctccgctg cgagcgcctt cgcctcgtag cgagtcgagg cccagccgga ccatggttcg
     1141 cgccaatcgc acaccgattg cgcgccccac tcgaaatcag gcgattccaa tacacgcgcc
     1201 agcgtccagc cggcattgtc gtcgatgtcg gtggcgaagc ggatttccgc gccggggcgc
     1261 atgatgcgtg cgagacgttt tagcatcgtg tcggagagaa aacggcgttt gcgttggcgt
     1321 cgcttcggcc aggggtccgg ataaagcaga taggcgccgg cgaggctctg tggcggcaga
     1381 gcttcgatga tggcgccagc gtcgccttga tagagacgga cgttatcgag gtctttgtga
     1441 gcgatcagcg tcaaaacctt gacgacgcca ttttcaaaaa attcgcagcc gatgaatccc
     1501 tcgtcgggat gcgccgcagc ttcagcggcc agatgttcgc cgccgccaaa accaatttcg
     1561 agccagatgt cgcgcggcgc gttcggaaac aaactaggtg cagagatgat cggctgtgcg
     1621 ggatcaagtg cgaggcgcgg caggagagtt tcgcgcagat tgacctgcag ggcgctaagc
     1681 tttttgccct tgcggcgccc gtaaagccgc gtcgcaaggg gatgttcgtc gatcatgtgc
     1741 gatgtcgcgt gtgcttggcg aaacgtcgct tagctgaagc tcgcgcggag cttgtcggcg
     1801 agatccgtct tctcccacga gaaaccgccg tcggcttcgg gtgcccggcc gaaatggcca
     1861 taggccgagg tgcgcgcgta aatcggcttg ttcaattgca gatggtcgcg gatgccgcgg
     1921 ggcgagagac gcatcacctg cggcagcaca gcttcaagct tgtcttccgc gactcggccg
     1981 gtgccatgcg tatcgatgta aatcgacagc ggctcagaat agccgatcgc ataggcaagc
     2041 tgaatcgtga cccggtcggc gagatcggcc gcgacgatgt tcttggcgag atagcgcact
     2101 gcataagctg ccgagcgatc gaccttggtt ggatctttgc cggagaaggc gccgccgccg
     2161 tgcggggccg cgccgccata cgtatcgacg atgatcttgc ggccggtgag gccggtatcg
     2221 ctgtcggggc cgccgatgac gaacttgccg gttgggttga cgtgccagat cgtctgctcg
     2281 ttgatccact gcgccggcag cgtcctgcgg atatagggct cgacgatcgc gcgcacgtcg
     2341 tccgacgaca gattcggatc aagatgctgc gtcgagagca cgatctgcgt tgccgcgaca
     2401 ggtttgccgt cggcatattg gatcgtcacc tggcttttgg cgtcggggcc aagcttggca
     2461 gcgtcgcctt cgcccgcgtg acgggccttg gcgagcactt cgagaatttt gtgcgaataa
     2521 taaagcggcg ctggcatcag ctccggcgtt tcgcggcagg cgaagccgaa catgatgcct
     2581 tgatcgccgg cgccttcatc cttgttgcct gcggcatcga caccttgcgc gatatcgacc
     2641 gattgggcgt gcagcaaaac ttcgaacttg caatgctccc aatggaagcc gtcctgctca
     2701 tagccgatcg ccttgatcgc cttgcgcgcc gtatgttcga tgacatcgga ggagagtttg
     2761 gggccgcgcg tctcgccggc aataacgacg cgattggtcg tcgccaaagt ctcgcaggca
     2821 acgcggactt cgtaaggatc aatgccggct ttggcggatt cacggaagtg aaggtcgacg
     2881 atttcgtcgg agat
//
LOCUS       DASWOG010000086         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_185400,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000086 DASWOG010000000
VERSION     DASWOG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185400"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2426)
                     /locus_tag="VGB93_01760"
     CDS             complement(63..2426)
                     /locus_tag="VGB93_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0144887.1"
                     /translation="MSSLMLCPSWARSGAARANRGHSLHLDRNGLRAFALLFGLAGWQ
                     SSAAAQSGAAVTDALPTIDVNAAPTQATGATTPAPQPALEQPAGETIYTQSRDSYDDK
                     PALTIGDMLSQTPGVTFQTGNGPRDLSISIRGSNDHMAFGIRNIQILEDGFPLVQPDG
                     RARADLIDPHAYGSIDTFQGPASTLYGNYATGGALFLHTRNPADVNGIEIGSDFGNFG
                     TINNYVTIGETGKNYQLFMFGSDVRGETGIAHTQYDTPTENILLRVDASPTDRFIFKL
                     INNNTITDQSVRLSLDQFEINPFQKDCAGLQSAACGSVTLLRNGAAGATVNVSPEQAG
                     LRRDDWRMLEGVRWEHDLDANTLVRTQVTYDRLKIDQPNTAVSEAGNFDSVDLRSDIT
                     NNSAVFSRPLTSFAGIDFNYLDFGDHFHNLLPTGDAAVGPLSQTLFGHEWNASARFQE
                     DLQFAKNWDFVTGLGGEFTHIDGTETNFNTSLPSDAASSIIPADRSFFNLAPEASLIY
                     KPNQEWTLHSRVGTGYGTPQPNALFTTPQGTFGNNTQLKAQSNVGFDLGAQWTPLRSV
                     SIQATGFYEFFRNEQITQSAGAGLQNFTFNAPGSQHRGVELAALWQPLPQQLPEAHLQ
                     LSYLYDNQVYTNFSDTLSNATTTASFNRDGNAIPGVVPHFLDARFLYDEDHGPLKGVG
                     GFVELVYRSSFYLDNANLLQAPGYEIVNAGLHYNPPVEAGPLHKAHIYFEVENLLDKT
                     YVGSASNITDTLGTNGQVGDAATLAAHTGSIFAGNPRGFYGGLKLSF"
     gene            complement(2710..3561)
                     /gene="nadC"
                     /locus_tag="VGB93_01765"
     CDS             complement(2710..3561)
                     /gene="nadC"
                     /locus_tag="VGB93_01765"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0144888.1"
                     /translation="MNLAPLSRLILEPLVRAALVEDLGLAGDITTNAIVPPEVETQAL
                     FVARKPGVVAGLDLAKLSLELVDPSLTFSVAKPDGTHVAAGETIATVSGKAAAILIGE
                     RVALNFLGHLSGIATATAKFVKAVEGHNAKIVCTRKTTPGLRAIEKYAVRAGGGQNHR
                     FGLYDEVLIKDNHIAIAGGVTVALRRAKQHVGHMVKIEIEVDSLEQLREALSEKPDVI
                     LLDNMTPAQLAEAVKIVDGRAITEASGGVSLETAAAVAASGVDLIAIGWLTHSAPTLD
                     IGLDFPD"
BASE COUNT          689 a          999 c         1190 g          763 t
ORIGIN      
        1 ggttatgggt ggttcgcgga aaatagcccg ccacgggtga ggccgtttgt ggcggggctt
       61 tctcaaaatg agagtttcaa gccgccgtaa aagccgcgtg gattgcccgc aaagatcgat
      121 ccggtatgtg cggccagtgt cgcggcatcg ccgacttgac cgttcgttcc caaagtatcg
      181 gtgatatttg aggcggatcc gacataggtc ttgtcgagca ggttctcgac ctcaaaatag
      241 atatgcgcct tatggagcgg gccggcctct acaggcgggt tatagtgaag gccggcgttg
      301 acgatttcgt agcccggcgc ctgaagcagg ttggcgttgt cgaggtagaa cgagctgcgg
      361 tagacgagtt ccacgaagcc gccgacgccc ttcagcgggc cgtggtcttc atcatagagg
      421 aagcgcgcgt cgaggaagtg cggcaccacg ccgggaattg cgttgccgtc acgattgaag
      481 ctcgccgtcg tggttgcatt cgagagcgtg tcgctgaagt ttgtgtagac ctggttgtcg
      541 taaagatagg acaattgcag gtgtgcctcc ggcaattgct ggggcagcgg ttgccagagc
      601 gccgcaagct ctacgccccg atgctgcgag cccggcgcgt tgaacgtgaa attctgcaag
      661 cccgcgccgg cgctctgcgt aatttgctca ttgcgaaaga attcgtagaa cccggtcgcc
      721 tgaatgctca cggagcgaag gggcgtccat tgcgcgccga gatcgaaccc gacgttcgac
      781 tgcgccttca attgcgtgtt gttgccaaac gtgccttgcg gcgtggtgaa caaggcgttc
      841 ggttgcggcg tgccgtagcc ggtgccaacg cggctgtgca acgtccattc ctggttgggc
      901 ttgtagatca gcgaggcttc cggcgcgaga ttgaagaagc tccgatcggc cgggatgatg
      961 cttgaggctg catcgctcgg cagactcgtg ttgaaatttg tttcggtgcc atcgatatgg
     1021 gtgaattcgc cgccaaggcc ggtgacgaaa tcccaattct ttgcgaattg cagatcttcc
     1081 tgaaagcgcg cgctggcgtt ccactcgtgg ccgaacaagg tttgtgacaa cgggccgacc
     1141 gccgcgtccc cggtcggcaa taaattgtgg aaatggtcac cgaagtcgag atagttgaag
     1201 tcgatgccgg cgaaggatgt caatggccgc gagaagaccg cgctattgtt cgtgatgtcc
     1261 gaacgcaaat cgacggaatc gaagttgcca gcctctgaaa ccgccgtatt cggttgatcg
     1321 atcttcagcc ggtcgtaggt cacctgcgtg cgcacgagcg tgttggcatc gagatcgtgt
     1381 tcccagcgta cgccttcgag catgcgccag tcgtcacgcc gcaatccggc ctgctcgggg
     1441 ctgacattga ccgtcgcgcc ggcggcgcca ttacggagca aggtgacgct cccgcaggcg
     1501 gcgctttgca ggccggcgca gtctttctgg aagggattga tctcgaactg gtcgagcgac
     1561 aggcggaccg attgatcggt gatcgtattg ttgttgatga gcttgaagat gaagcgatcg
     1621 gtcggtgatg catcaacccg cagcaggatg ttttccgtcg gcgtgtcata ttgcgtatgc
     1681 gcgatgccag tttcgccccg cacgtcgctg ccgaacatga aaagctggta gtttttgccg
     1741 gtctcgccga tcgtgacgta attgttgatc gttccaaagt tgccgaaatc gctgccgatt
     1801 tcgatgccat taacatcggc tggattgcgt gtgtgcagga agagtgcgcc gcccgtcgcg
     1861 taattgccgt aaagagtcga ggcgggcccc tggaaagtgt cgatagagcc ataggcatgt
     1921 ggatcgatca ggtcggcccg ggcgcgtccg tccggctgaa ccagtgggaa gccgtcttca
     1981 agaatctgga tgttgcggat gccaaaggcc atgtggtcgt tggagccgcg aatggagatc
     2041 gagaggtcgc gtggtccgtt gccggtctgg aacgtcacgc ccggcgtttg cgacagcatg
     2101 tcgccgatgg tgagcgcggg cttgtcgtcg tagctgtcgc gtgactgcgt atagattgtt
     2161 tcgccggccg gctgttcgag agcgggttgg ggtgcgggcg ttgtggcgcc cgtcgcttgc
     2221 gtcggcgctg cattgacatc gattgtcggc agggcgtcgg tgacagccgc gccactctga
     2281 gcagcggcgg aactttgcca gcccgcgagg ccgaacagaa gggcgaacgc ccttaagccg
     2341 ttgcgatcca gatgaagaga atggccgcga tttgcgcgcg cagcaccgga ccgtgcccac
     2401 gatggacaca gcattaaaga tgacatcgaa atttccccaa accagctcgc agccgcggaa
     2461 acggctgctt tgatacggcg ccgagcgtcg cacccccgat gcgattggtg ggtcttaaaa
     2521 ccttccgatc tgaagcattg gcgggtgcca acgcggatcg gtcccgatcg ctaagatgat
     2581 tctttatttg ccgcggatgg caaccgcaaa tgacttataa gtcattgtta tagctagaac
     2641 tattcaaaaa gtatcatact tcaggtatac ttgcgggcgg cgccgttgga cggtaatcgg
     2701 cgcgattggt cagtcgggaa aatcgagccc gatatcgagg gtcggggcgc tatgcgtgag
     2761 ccagccgatg gcgatgagat cgacaccgct cgccgcgacc gccgcggcgg tctctagcga
     2821 gacgcccccg gacgcttcgg tgatggcgcg gccatcaacg atctttacgg cctccgccaa
     2881 ttgcgcgggg gtcatgttgt cgagcaggat gacatccggc ttttcggaga gcgcctcgcg
     2941 taattgttcg agcgaatcga cctcgatctc gattttgacc atatggccga catgctgttt
     3001 cgctcgccgc aacgccaccg tcacgccgcc ggcgatggcg atgtgattgt ccttgatcag
     3061 aacctcatca tagaggccga agcgatggtt ctggccgcca ccagcgcgca ccgcgtattt
     3121 ctcgattgcg cgcaggcccg gcgtggtttt ccgcgtgcag acgatctttg cgttgtggcc
     3181 ctcgacggct ttgacgaatt tggccgtcgc ggtggcgatg ccgctcagat ggccgagaaa
     3241 attcagcgcc acgcgctcgc cgataaggat cgcagccgcc ttgccgctga cggtcgcgat
     3301 cgtctcgcca gccgcgacat gtgtaccgtc gggcttcgcg acggaaaagg tcagcgacgg
     3361 atcaacaagt tcgaggctga gtttcgccag atcgaggccc gcgacgacgc ccggcttgcg
     3421 cgcgacgaat agcgcctgtg tttcgacttc gggcggcacg atcgcgttgg tcgtgatgtc
     3481 gccggcaagg ccgagatcct cgacgagcgc cgcgcgaacc aagggttcga ggatgagccg
     3541 cgacaggggc gccagattca tcaggcgacg tcggcgagag aaagttcacg cgcgacggca
     3601 acggcctgcg cagtgtatat gtcgcgcggc catgttggag c
//
LOCUS       DASWOG010000087         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_222045,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000087 DASWOG010000000
VERSION     DASWOG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222045"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1013)
                     /gene="cysQ"
                     /locus_tag="VGB93_01770"
     CDS             complement(159..1013)
                     /gene="cysQ"
                     /locus_tag="VGB93_01770"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0144889.1"
                     /translation="MSVQLSSKPLSLNYQAIAEDFAELAVAAGAAIMLFYNNDSNARQ
                     KGDTSPVCDADLAGEAVILAGLAARMPELPVVSEEAAAAGAQSTTNDTFILVDPVDGT
                     REFLAGKGEFTVNIGLVVRGEPKVGVVYAPALEQMWLAGDHAVTFPVAPGGKLPPPSE
                     RSRITVRKQDHENRVALTSWSHTDPRTSAFLDHLNIKERRMIGSSLKFCALADGSADI
                     YPRFGATMEWDTAAGDAILRAAGGIVLDREGQPLRYGKTATKFMNGSFVAWADPDTSV
                     VAADPRTA"
     gene            1346..>1885
                     /locus_tag="VGB93_01775"
     CDS             1346..>1885
                     /locus_tag="VGB93_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEY0144890.1"
                     /translation="MTEFGLGSLDLAALLCSRVCHDVISPVGAIVNGLEMLDEEKDPE
                     MRGFALDLVKKSAHTASARLQFCRLAFGAAGSAGAAIDTGDAEKVTRGLLEDDRTKLR
                     WNVPRILMAKDKVKLILNLCLIAAAAIPRGGVITVNVDTSEPTPHIEVEAYGTNARLA
                     SHVPQLLAGTPENNQVDAHG"
BASE COUNT          381 a          587 c          544 g          373 t
ORIGIN      
        1 tttcgctgaa aattcccgcc gcgaagtggg tctcatggtc atctcccgaa tgagccggcg
       61 cgcggcatgg tcaatcccca catcttcccg ccaagcggaa gacgcgccga gggccgcgaa
      121 tcgcgccccg actctcaacg accatagtta atgaaagatt acgccgttct tgggtccgca
      181 gcgacaacgc tcgtatcggg atcggcccag gcgacaaaag agccattcat gaatttggtc
      241 gccgtcttgc cgtagcgcag cggctgtcct tcgcgatcga ggacaatgcc gcccgccgcg
      301 cgcaaaatcg catcgccggc agccgtatcc cactccatcg tagcgccgaa acgcgggtag
      361 atatccgctg agccatccgc cagcgcgcag aacttgagtg aggacccgat catccggcgt
      421 tcttttatgt tcagatggtc caaaaacgcc gaggttcgcg gatccgtatg cgaccagctc
      481 gtcagggcga cgcggttctc gtggtcctgc ttgcgtaccg tgattctcga acgctccgac
      541 ggcggcggca atttcccgcc aggcgcaacc ggaaaagtca cggcatgatc acccgcgagc
      601 cacatctgtt cgagagccgg cgcatagacg acgccaacct tgggctcgcc gcgtacaacg
      661 agtccaatat tgactgtgaa ttcacctttg ccggcgagga attcgcgcgt gccgtcgact
      721 gggtcgacca gaatgaacgt gtcgttggtg gtcgattggg cgcccgccgc cgcggcttct
      781 tccgaaacca ccggcagctc tggcatccgc gcggcgagcc ccgcgagaat gacagcctcg
      841 cctgcaaggt cagcgtcgca gactgggctc gtatcgccct tctgccgcgc attactgtca
      901 ttattgtaga acaacatgat cgccgcgccg gcggcgaccg cgagctcggc gaaatcctcg
      961 gcgatcgcct gatagttcag gcttaacggc tttgagctta attggactga cattggatcc
     1021 cgcagacccc gcgactcact tcgccgctta tgaggtgaag atattggctt cccggcggct
     1081 aatgaaaggc ctatcggcgc aatggttgca aacgtccttg tcgggtgtaa caaaatgccg
     1141 tcgctgcgca agtcgattcg ccgcgggttt ctgtcgcaat gttgagattt atcttgcact
     1201 tgcgaagaac ttacgccgct atgccgcgct gcaacagaac gaattcgatc aagcggcgcc
     1261 catttaatgg cacgtcggct tgcttctgga tgggtctttc gcagagcttg gactgaaagc
     1321 ccacgctaca tccaaaggac ggaacatgac ggaattcggc ctcggctctc tcgatctcgc
     1381 cgccctctta tgctcgcgcg tctgtcatga cgtgatcagc ccggtggggg ctatcgtcaa
     1441 cgggcttgaa atgctcgatg aagagaagga tcccgagatg cgcggcttcg cgctcgatct
     1501 tgtcaagaag agcgcccata ctgcctcggc gcggctgcaa ttctgccggc tggctttcgg
     1561 cgctgcgggt tctgcgggcg ccgcgatcga caccggcgat gccgagaaag tcacgcgcgg
     1621 cctcttggaa gatgaccgca ccaagctgcg ctggaacgtg ccacgcatcc tgatggccaa
     1681 ggacaaggtc aaattgatct tgaatctctg cctgattgcc gcagcggcaa tcccccgcgg
     1741 cggcgtgatc accgtgaatg tcgatacaag cgaacccacg ccgcatatcg aagtcgaagc
     1801 ttacggaacc aatgcgcgcc ttgcgagcca tgttccacag cttctcgccg gcacaccgga
     1861 aaataaccag gtcgacgcgc atggc
//
LOCUS       DASWOG010000088         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_93817,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000088 DASWOG010000000
VERSION     DASWOG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93817"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VGB93_01780"
     CDS             complement(<1..707)
                     /locus_tag="VGB93_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144891.1"
                     /translation="MSNSGFDTVISQRNKWLTLAATVIGSAIVLLDSTVVNIALPAMQ
                     RALHADAAATQWVVNAYLLLLGPLVLIGGAAADLYGRRRMFVLGIAIFAAASIVGGFS
                     PNVTVLIACRAAQGFGGALLSPASLAILQTTFSEHERNRAIGIWAGAGALMMSVGPLL
                     GGWLVDQISWRAIFFINIPLAAIAIGLALRFGEESFSPQAKRLDWAGAISVAIGLAAL
                     TWSLSAVPKSGFHDPSV"
     gene            complement(707..1507)
                     /locus_tag="VGB93_01785"
     CDS             complement(707..1507)
                     /locus_tag="VGB93_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0144892.1"
                     /translation="MSLTVTILGCGSSAGVPRVGQGWGACDPNNPKNRRRRCALFVER
                     SNADRAKTQVLIDMGPDLRAQLLDLGVERLDAILLTHSHADHTHGIDEIRPLVIMHKQ
                     RIDLHMDADTSAVVRRHFGYIFETPAGSYYPPLLNDVRLMPGEAVTIGGPGGDITFLP
                     FRLHHGEIDALGFRIGDFAYCPDLNAIPAESEVYLHSLKIWLVDALRYKPHPSHFSVA
                     EALGWIDKLKPQRAILTNLHTDLDFEQLQAKLPPHVEPAFDGMRFELL"
     gene            complement(1504..2298)
                     /locus_tag="VGB93_01790"
     CDS             complement(1504..2298)
                     /locus_tag="VGB93_01790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0144893.1"
                     /translation="MLIDSHCHLDFPDFAAECDNVVARARDAGVKRMITISTLVSKFA
                     EIRALAETYDDVYCTVGTHPMNASAEQVSVEDLVALAQYEKCVGIGEAGLDYHYDDTP
                     RDLAAQVFRTHIAAARQTELPLVIHARDADTDMAAILEDEMGKGLFKAILHCFTSTRK
                     LAETGLALGLYVSFSGIVTFKNSDELRAIARDVPDDRLLVETDAPFLAPVPYRGKRNE
                     PAYVAETAKIVAEVRGVTPTQLGALTTANVLRLFSKMPALAAASAA"
     gene            complement(2298..3854)
                     /gene="metG"
                     /locus_tag="VGB93_01795"
     CDS             complement(2298..3854)
                     /gene="metG"
                     /locus_tag="VGB93_01795"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0144894.1"
                     /translation="MAERPTFYITTAIPYANGAPHIGHAYERIATDAIQRFKRLDGGD
                     VLFCTGMDEHGQKMQQTAAREGITPLELADRTAAQFAAMGAALNAEADVIVRTTSEAH
                     REAAQEIWRRMTANGDIYLSKYPGWYSVRDEAFFDESELTKGEGGKYFAPSGAPVEWV
                     EEESYYFKLSAYQGRLLAHYRDNPNFIVPETYRNEIVAFVERGLTDLSISRSTFDWGI
                     PVPGDPKHVMYVWVDALTNYITGTGFPWQQDDSRKKFWPADAHVIGKDITRFHAIYWP
                     AFLMSAGIDVPKQIVVHGFLFNRGEKMSKSVGNVISPADLIATYGVDPLRYFFLREVP
                     FGQDGNYSHESIVNRINADLANDFGNLAQRSLSMIGKNFDGRLPVPGALTDADKAILE
                     AADALLNKAREAMKGFALHTILADIWRVVGNANRYFASEEPWTKKKTDPERMGTILYV
                     TAQVLRIVAILAQPFTPQGAERLLDLLAVAPEHRGFAFAHQADKDAPGRTLPEPSPVF
                     PRYVEEPAKP"
     gene            complement(3885..4964)
                     /locus_tag="VGB93_01800"
     CDS             complement(3885..4964)
                     /locus_tag="VGB93_01800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY0144895.1"
                     /translation="MARKPLLEAAPEADRIEATPHPRETLDFFGHGEAEQGLLNAYRA
                     ARLAQAYIIGGQPGIGKATLAWRFARLLLAHPDPTAREVQEADDLSIGADDPVMHKVA
                     ALSHGDVVLLRREWNEKSKRHYTEIRVDDVRRASHLFRSASAAGGYRICIVDAADDLN
                     NSAANALLKLVEEPPPRSLFLIVSHRPGLLLPTLRSRSQMLMLRPLETDEILRVIAAL
                     GEPWTEADAAERRVAAERAHGSVATALSLLADEGIALDNQVRALLGALPALDWRAVHA
                     LADRITGRDQTADCDRVMTAVVDWIDERLRAEAASGPARLAPLAEVWEKAADAAREAE
                     ALNLDKRPVILSIFAELAAARALSA"
     gene            complement(4954..>5186)
                     /locus_tag="VGB93_01805"
     CDS             complement(4954..>5186)
                     /locus_tag="VGB93_01805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589585.1"
                     /note="catalyzes the reversible phosphoryl transfer from
                     adenosine triphosphate (ATP) to thymidine monophosphate
                     (dTMP) to form thymidine diphosphate (dTDP); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEY0144896.1"
                     /translation="RRGAGQASDRFEGEGLAFHEALRKAYLAIAAAEPERCVLVDADH
                     SEEEVAQAIWDTVSYRLFESQPQSEAEALNGA"
BASE COUNT         1056 a         1574 c         1601 g          955 t
ORIGIN      
        1 aatacgcttg gatcgtgaaa tcctgacttc ggaacggcgc tcagagacca tgtcagcgca
       61 gccaaaccga tcgcaaccga gatcgcaccg gcccagtcga gtcgcttcgc ctgagggcta
      121 aagctttcct cgccgaagcg aagcgccaat ccgatcgcaa tggctgccag cggaatattg
      181 atgaaaaaga ttgcccgcca cgatatctga tcgaccagcc agccacccag caatgggccg
      241 accgacatca tcagagcccc cgcgcccgcc catattccaa tggccctgtt ccgctcatgc
      301 tcactgaagg tcgtctgtaa aattgccagg ctagccggcg acaacaaagc accaccaaaa
      361 ccttgcgccg cgcggcaagc gataagcacc gtgacgttcg gcgaaaaccc gccgacgatg
      421 gaagcggcag caaagatcgc gatgccgagg acaaacattc gccgtctgcc ataaagatcg
      481 gccgccgctc caccgatcag cacgagcggc ccgagcagca acaagtaggc gttgacgacc
      541 cattgtgtcg cagccgcatc cgcatgaagc gcgcgctgca tcgccggcag cgcgatgttt
      601 accaccgtgc tatcgaggag aacgatggcc gacccgatca cggtcgccgc caacgtcagc
      661 catttattcc gttgtgagat caccgtgtcg aaaccagaat tcgacatcac aagagttcga
      721 aacgcattcc gtcgaaggcc ggctcgacat gcggcggcag tttagcttgc aattgctcaa
      781 aatcgagatc ggtgtgcagg ttcgtgagga tcgcgcgttg cggcttgagc ttgtcgatcc
      841 aaccaagcgc ttcggccacc gaaaaatggc tcggatgcgg tttgtagcgc aaggcatcga
      901 cgagccaaat cttcaggcta tgtaggtaga cttcgctttc ggccggtatg gcattcaaat
      961 ccgggcaata cgcaaaatcg ccgattcgaa agccaagcgc atcgatctcg ccatgatgca
     1021 gtcggaacgg caaaaatgtg atgtcgccgc ctggaccgcc gatcgtcacg gcttcgccgg
     1081 gcatcagccg cacgtcattc agcaacggcg gataataact accggcgggc gtctcgaaaa
     1141 tatagccgaa atggcggcgc acgacggctg acgtgtcggc atccatgtgg agatcgatgc
     1201 gttgcttgtg catgatgaca agcgggcgga tttcatcgat tccgtgcgta tggtcggcat
     1261 gtgaatgagt aagcagaatg gcgtccagcc gctcgacgcc gaggtcgagc aattgggcgc
     1321 gcaaatccgg ccccatgtcg atcagaacct gcgttttggc gcggtcggcg tttgatcgct
     1381 cgacaaaaag tgcgcaacgg cggcggcggt ttttcggatt gttcggatcg caggcgcccc
     1441 agccctgccc aacccgcgga acgccggcgg acgagccaca gccgagaatc gtaaccgtca
     1501 ggctcatgcc gcagatgcgg cggcgagcgc cggcattttt gaaaacagcc gcaggacatt
     1561 cgcggtggtc agagcaccga gctgcgtcgg tgtaacgcct ctgacttcag cgacgatctt
     1621 tgcggtctcg gcgacatagg ccggttcgtt gcgcttgcca cgataaggga cgggcgcaag
     1681 gaatggtgcg tcggtctcga cgagcaggcg atcgtcaggc acatcgcggg cgatggcgcg
     1741 caattcgtct gaattcttga acgtcacgat gccggaaaag gacacgtaga gcccgagcgc
     1801 gagaccggtt tcggcgagtt tgcgcgtcga ggtgaagcag tgcaggatcg ccttgaaaag
     1861 ccccttcccc atttcgtctt ccaggatcgc ggccatgtca gtgtcggcat cgcgcgcatg
     1921 gatgacgagc ggcagctccg tttgccgcgc cgcggcgata tgcgtgcgaa aaacttgcgc
     1981 ggcgaggtcg cgcggcgtgt cgtcataatg ataatcgagg cccgcttcgc caatgccgac
     2041 gcatttttcg tattgcgcca gcgcgacgag gtcttcgacg gaaacctgtt cggcactggc
     2101 attcatcgga tgcgtgccga cagtgcaata gacatcgtca taggtttccg ccagcgcgcg
     2161 gatctcggcg aatttcgaaa ccagcgtcga aatcgtgatc atgcgcttga cgcccgcgtc
     2221 acgcgcgcgt gcgacgacat tgtcgcactc ggcggcaaag tcgggaaaat cgagatggca
     2281 gtgcgagtcg atcaacatca tggcttggct ggttcttcga cataacgcgg gaagaccggc
     2341 gaaggttcag gcagcgtgcg tcccggcgca tccttgtcgg cttggtgcgc aaaggcgaaa
     2401 ccgcggtgct ccggcgccac ggcgagcaga tcgagcagcc gctccgctcc ctgcggcgtg
     2461 aagggctgcg ccagtatcgc aacgatgcgc agcacttgtg ccgtgacata gaggatcgtg
     2521 cccatccgct ccggatcggt cttcttcttc gtccagggct cttccgaggc aaaatagcga
     2581 ttagcattgc cgacaacacg ccagatgtcg gcaaggatcg tatgcagggc aaagcccttc
     2641 atcgcctcgc gcgccttatt caaaagcgca tcggcggctt cgaggattgc cttgtcggcg
     2701 tcggtcaagg cgcccggcac cggtagcctg ccgtcgaaat tcttgccgat catcgaaagt
     2761 gaacgctgcg ccagattgcc gaaatcgttg gcgagatcag cgttgatgcg attgacgatc
     2821 gactcgtgcg aataatttcc gtcctggccg aacgggactt cgcgcaggaa gaaatagcgc
     2881 aagggatcga cgccataggt cgcgatgaga tcagccgggc tgatgacatt gccgaccgac
     2941 ttcgacattt tctcgccgcg gttgaacaga aagccgtgga cgacgatctg cttcggcacg
     3001 tcgatcccgg cggacatcag gaaagccggc caataaatcg cgtgaaagcg tgtgatgtct
     3061 ttgccgatga catgcgcgtc agccggccag aattttttcc gcgagtcatc ctgctgccac
     3121 ggaaagccag tgccggtgat gtaattggtc agcgcatcga cccagacata catcacgtgc
     3181 ttcggatcgc ctggcacggg aatgccccaa tcgaaggtcg aacggctgat cgagagatcg
     3241 gtcaggccgc gctcgacaaa tgcgacgatt tcgttgcgat aggtttccgg gacgatgaag
     3301 tttggattgt cgcgataatg cgcaagcagc cggccctgat aggcggaaag cttgaagtaa
     3361 tagctctctt cctcgaccca ttcgaccggc gcaccgctcg gcgcaaaata tttgccgccc
     3421 tcccctttgg tgagttcgga ttcgtcgaaa aaggcttcgt cgcggaccga ataccagccg
     3481 ggatattttg acagataaat atcgccattg gccgtcatgc ggcgccagat ttcttgcgcg
     3541 gcctcgcgat gcgcctcgct cgtcgtgcgt acgatgacat cggcctcggc attcagtgcc
     3601 gcgcccatcg cggcaaattg cgccgcggtg cggtcagcga gctccagcgg agtgatgcct
     3661 tcgcgcgccg ccgtctgctg catcttctgc ccgtgctcgt ccataccggt gcagaacaga
     3721 acatcgccgc cgtcgagacg cttaaaacgc tggatggcgt cggttgcgat gcgctcatag
     3781 gcgtggccga tgtgcggcgc gccattggcg taaggaatcg cggtcgtgat gtagaaggtc
     3841 gggcgctcag ccatgctcac tcatcggccg tgcttcgacg gggcttaggc ggaaagcgcc
     3901 ctggccgccg cgagttcggc aaaaatcgac aggatgacgg gccgcttgtc gagattgaga
     3961 gcctcggctt cccgcgccgc gtcggcggct ttttcccaca cctctgccaa gggtgcaagg
     4021 cgagccgggc cagacgcggc ttcggcacgc agtctctcgt cgatccaatc gacgacggcg
     4081 gtcataaccc ggtcgcagtc ggccgtctga tcgcggcccg tgatcctatc ggcaagtgcg
     4141 tgaaccgcgc gccaatcgag cgccggcaac gcgccaagca gcgctcgaac ctgattatcg
     4201 agggcaatgc cttcgtcggc gagaaggctt agcgcggtgg cgacggagcc atgcgcgcgc
     4261 tcggcggcaa cccggcgttc ggctgcatcc gcctcggtcc agggttcacc gagcgccgcg
     4321 atgaccctca aaatctcgtc ggtttcgagc gggcgcagca tcagcatctg cgagcgtgag
     4381 cgcagggtcg gcagcagcag gcccggacga tgcgagacga tcagaaacag cgagcgtggc
     4441 ggcggctcct caacgagctt caggagcgca ttggcagccg aattatttag gtcgtcggcg
     4501 gcgtcgacga tgcagatgcg atagccgccg gccgcggagg cggagcgaaa gagatgcgac
     4561 gcccggcgca catcgtcgac gcggatctcg gtgtaatggc gcttcgattt ttcgttccac
     4621 tcccgccgca gcaggacgac atcgccatgc gaaagcgcag cgaccttatg catgaccgga
     4681 tcatctgcgc caatgctcag atcatcggcc tcctgaacct cgcgggccgt ggggtccgga
     4741 tgggcgagca gtaagcgggc gaagcgccag gcgagcgtcg ccttgccgat gcccggctgg
     4801 ccgccgatga tataggcttg cgccaagcgg gcggcacgat aagcgttgag gagaccctgc
     4861 tccgcctcgc catggccgaa aaaatccaga gtttcgcgcg ggtggggtgt cgcctcgatg
     4921 cgatcggctt ccggcgcggc ttccaacagc ggcttacgcg ccattcagag cctcggcctc
     4981 gctctgcggc tgcgactcga acagcctgta gctgaccgta tcccaaatcg cttgcgccac
     5041 ttcctcttca ctgtggtccg catccaccag gacgcaccgc tcgggttctg cggcggcgat
     5101 cgcaagatag gccttacgca aagcttcatg gaaggcgagc ccctcacctt caaaccggtc
     5161 ggacgcctgc ccggcgccac gccgcg
//
LOCUS       DASWOG010000089         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_38596,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000089 DASWOG010000000
VERSION     DASWOG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38596"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..488
                     /locus_tag="VGB93_01810"
     CDS             69..488
                     /locus_tag="VGB93_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0144897.1"
                     /translation="MEMKQGQTANGATPSDTEGEPYMTIGEMAREFNVSLRTLRFYED
                     RGLLHPLRNGVTRLYPTRDRLHLQLILRGRHLGFTLSEIATLLSDQDLSGEGARLKLA
                     PDQIRAQIRHLERQRETLDAAIAELRLAEIQLTQTPS"
     gene            complement(491..1732)
                     /locus_tag="VGB93_01815"
     CDS             complement(491..1732)
                     /locus_tag="VGB93_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0144898.1"
                     /translation="MAGGTFRSLKVFNFRVWAAGSLVSNIGSWMQRTAQDWLVLTALT
                     HNNASAVGIVVALQFAPQVLLLPVTGYAADHFDKRKLIFATQTAMGLLALALGILVVT
                     GLVRLWQVDIFAFLLGCAAAFDMPARQAFVSELVGEADLGNALALNSTSFNAARMIGP
                     AAAGLLIAAVGTGAVFLINAASFLAVLASLVCLRGDALHSRDRSERRRGGLIEGFRYV
                     WQRGDLKTILLMLLVVGMFGLNFPIFISTMAVSVFHAGAGRFGFLTSMMALGSIAGAL
                     FTARRGNPNMQILLVGALLFGLACGLAALTPTYIVFGLVLVIVGFAVQTFTTSTNSLV
                     QLSIPPQMRGRVLAILLAIALGGTPLGAPIIGWVADRLGPRVALGLGAASGFVAAMIA
                     CAYLVRYSSRPHIRPDKAREA"
     gene            complement(1737..2066)
                     /locus_tag="VGB93_01820"
     CDS             complement(1737..2066)
                     /locus_tag="VGB93_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144899.1"
                     /translation="MQWPHEISYFFGGAFLANAVPHFVSGIMGRPFQSPFAKPPGEGL
                     SSSTVNVLWGFANFAFAYLLLARVGNFDLYSADHVIALGAGVLFISVMCARLFGRFHG
                     GNSPTGA"
BASE COUNT          527 a          660 c          644 g          337 t
ORIGIN      
        1 caattttaag ttgtgcaacg ccgtgattca gtccaccttt gtctgcgctc gacggaatgg
       61 agtgagcgat ggaaatgaag caaggccaaa ccgccaatgg agctacgcca tcggacaccg
      121 aaggcgagcc ctatatgacc atcggcgaga tggcgcgtga gttcaatgtg agcctgcgga
      181 cgctgcgctt ttatgaggat cgcggccttc tacatccgct gcgcaatggc gtgacacgcc
      241 tctatcccac ccgcgaccgc ctgcatctgc agctgattct aaggggccgc catctcggct
      301 tcacattgag cgaaatcgcc acgctgctct ccgatcagga cctctccggc gagggagccc
      361 ggcttaaatt ggcgccggat cagatccggg cgcaaatccg ccatctcgag cgccagcgcg
      421 agaccctcga tgccgcaatt gccgagttgc gtctagccga aattcagctg acccaaacgc
      481 cgagctgatt tcaggcttct ctggccttat cgggccggat gtggggacgc gaagaatatc
      541 tgacgagata ggcgcacgcg atcatcgccg cgacaaaacc tgaagcagca ccaaggccga
      601 gcgccacgcg cggaccaaga cgatccgcga cccacccgat gattggcgct cctaacggcg
      661 ttccacccaa cgcaatggcg agcagaatgg ccaggacacg cccgcgcatc tgcggcggga
      721 tcgaaagctg aacgaggcta ttggtcgagg tcgtgaaggt ctgcacggcg aagcctacaa
      781 tgacaagcac gaggccaaag acaatatatg tcggcgtcag cgcggcgagc ccgcaagcca
      841 gaccgaaaag cagcgcaccg acgagcagaa tttgcatatt cggattgccc cgccgcgccg
      901 tgaatagcgc gccagcgatg gagcctaacg ccatcatcga ggtcagaaat ccgaaccggc
      961 ctgcgcccgc atgaaaaacc gagaccgcca tcgttgagat aaaaatcgga aaattaagcc
     1021 cgaacatgcc gaccaccagg agcatcagca gaatggtctt gagatctcct cgttgccaga
     1081 catagcgaaa cccttcgatc agaccgccgc gtcgccgctc acttcgatca cgtgaatgga
     1141 gtgcgtcgcc gcgcaggcac acgagggaag caagcaccgc aagaaacgac gcagcattga
     1201 tcaggaagac cgcgccggtg ccaacggccg cgataagaag acccgcggcg gccggcccga
     1261 tcatccgcgc ggcgttgaac gacgttgaat tcaacgcaag cgcgttgccg aggtcggcct
     1321 ccccgacaag ttcggaaaca aaggcctggc gtgccggcat atcgaaggcg gccgcgcagc
     1381 caagcagaaa cgcaaaaata tcgacctgcc acaaccggac gagacctgtg acaaccaaaa
     1441 tgccaagcgc caacgcgaga agtcccattg ccgtctgcgt cgcgaaaatg agcttgcgtt
     1501 tgtcaaagtg gtcggccgca taaccggtca cgggcagcag caaaacctgc ggtgcgaact
     1561 ggagcgccac gacgatgccg acggcagatg cattgttatg cgtcagtgcc gtcagcacca
     1621 gccaatcctg ggccgtgcgc tgcatccacg acccgatatt cgagacaagc gagccggcgg
     1681 cccagacacg aaagttgaaa accttcagcg aacggaatgt gcccccggcc atgccgtcag
     1741 gcgcccgtgg gcgaattgcc gccgtggaac cgcccgaaca ggcgcgcgca catcacactg
     1801 ataaacagga cgcccgcacc aagggcgatg acgtgatccg cggaatagag atcgaagttt
     1861 cccacgcggg cgaggagaag gtaggcgaag gcgaagttgg caaatcccca gagcacgttc
     1921 acggtcgagg atgaaagacc ttcgcccggc ggtttggcga atggactttg gaacggcctt
     1981 cccattatgc cgctgacgaa atgcggaaca gcattcgcga gaaaagcgcc gccgaaaaag
     2041 taggatattt cgtgaggcca ttgcatgctc aagcgccctt ttctaggagg ccgatgatct
     2101 cccgtgtcgt gcccgtctcg ccaaggcgcg gaaagatgcg cgtgaggctg ttttcgtggg
     2161 cggcgagg
//
LOCUS       DASWOG010000090         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_276954,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000090 DASWOG010000000
VERSION     DASWOG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276954"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..294)
                     /locus_tag="VGB93_01825"
     CDS             complement(34..294)
                     /locus_tag="VGB93_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEY0144900.1"
                     /translation="MWRVVLRPALLFLLPFMLYAVWLLLRRATPFGRDHWGGGVVSTL
                     TLLGLVIAVIGMFAFGIFADRHLGAYVPAHIENGVVVPGRMQ"
     gene            466..1887
                     /gene="pabB"
                     /locus_tag="VGB93_01830"
     CDS             466..1887
                     /gene="pabB"
                     /locus_tag="VGB93_01830"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY0144901.1"
                     /translation="MTAKLDGLWVYQLAWREPLDVARALAERPGFAFLDSAMFEPKLG
                     RYSFIGLEPFGIFTAVGRRAAWNGVTLEATPQDALRDLLSRFSFTPDPALPPFQGGVM
                     GAVPYEFGWEFDGLRGGRHVLAQDEPVSLGFYDLVLAFDHLLRRAYIFSSGLPETDDL
                     SRSARAAARIDHLLALLEHPGRIANAPPAIHGWASNFTPVEYHAAIEDVQAHIFAGDI
                     YQANIAQRFSAKLPEDFAPFGFYEKLRAANPAPFAAYLNLGETVIASSSPELLLRKRG
                     RGIETRPIKGTINRSPDPAKDARRAQALLKSEKDRAENIMIVDLLRNDLSRVSEPKSV
                     EVPVLCGLESYANVHHLVSVVTGELAQSRDIVDLIAATFPGGSVTGTPKRRAMEIIDA
                     IEKVSRGYYCGSILYIGFDGTIDMNIAIRTAVLKPGRASLHAGGGITSLSDPVAEYAE
                     TEIKVKRLFEAFGAAEELEEAAQ"
     gene            1884..2489
                     /locus_tag="VGB93_01835"
     CDS             1884..2489
                     /locus_tag="VGB93_01835"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0144902.1"
                     /translation="MSNRVLVIDNYDSFVFNVARYFEELGRDVVVRRNDEIDVAEIRR
                     AAPAAIVISPGPGTPRDAGISEAVVEELTGEIPILGVCLGHQAIGEVFGGRIDPAKRP
                     LYGRTSRLTHSGKGLFEGLPQPLVVGRYHSLVVERTPALETNLSIDAVSEEGEIMALS
                     HHTAAVYGVQFHPESILTEFGHALFANFLRLAMERKPRVLV"
     gene            2476..3309
                     /locus_tag="VGB93_01840"
     CDS             2476..3309
                     /locus_tag="VGB93_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0144903.1"
                     /translation="MFWFNGAIQEQPVVSFDLTDRGLNLGDGVFDTSLARNGHIFRRK
                     SHIDRMADAAEILGIPFDEMAAGTALDALAEAIGDGAVRLTLTRGPGPRGLSFPKNPA
                     PLLYGAAAAMHMPLFPVLKLGTTSIRRNETSPASRIKALPYLDSVLALEQASARGADE
                     VLFENTHSRVACLATANIFAIFGRQLVTPPLSDGALAGTTRAFVLSKAHGLHFDVAER
                     SLPLEKFSRADALFATSSLRLIAPCSQLDAITYPSEANEIVMELQASIKEVIASECGR
                     F"
     gene            3407..>3682
                     /locus_tag="VGB93_01845"
     CDS             3407..>3682
                     /locus_tag="VGB93_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0144904.1"
                     /translation="MLRLAILRHAEAVPLTDESDADRELTPAGWEMAERMGRYFRDAG
                     LRPDLALVSPAKRAKETFRGLERGAGVTFTVDFVGKLYSADLRTLEEV"
BASE COUNT          690 a         1113 c         1109 g          770 t
ORIGIN      
        1 gcgcaggcgc gggcgccgcg atctcggtca cgctcattgc atgcggccgg gcacgacgac
       61 gccgttttcg atgtgagccg gcacgtaagc cccgagatgc cggtcggcaa aaattccgaa
      121 ggcgaacatt ccaatcacag cgatgaccag ccccaatagc gtcaaggtcg aaacgacgcc
      181 gccgccccaa tggtcacggc caaaaggcgt cgcccggcgc aagagaagcc aaaccgcata
      241 aagcatgaag ggcaggagga atagaagcgc gggcctcagg acgacgcgcc acattcaaac
      301 ccccctcatg gcaagactag cttggcccta agctagggcg cccggcgccg ctttgcaaag
      361 ccgcgaggcg attgcgtaac cgtctcggaa tccacatatc tgcgtcggtt ttcctgttct
      421 tcgcgaaaat gctaccaaat ggcgccgaat ccggcgggaa atgacgtgac ggcaaagctg
      481 gacggacttt gggtttatca gctcgcatgg cgcgagccgc tggatgtcgc acgcgcgctc
      541 gccgagcggc ctggcttcgc atttctcgac agcgcgatgt tcgagccaaa gcttgggcgt
      601 tattcgttca tcgggttgga gcctttcggc atttttaccg cagtcgggcg tcgcgcggcg
      661 tggaatgggg taaccctcga ggccacgcca caggacgcgc tccgagatct gctcagccgt
      721 ttttcgttta cgccagatcc ggcgctgccg ccgtttcagg gcggggtaat gggtgccgtc
      781 ccttacgaat tcggctggga atttgatggc ctgcgcggcg gccggcacgt tttggctcaa
      841 gacgagcccg tttcgctcgg cttttacgac ctcgtccttg ctttcgatca tctgttgcgc
      901 cgcgcctaca tcttttcctc cggcttgcct gagacggacg acctatcccg ctcggcacgt
      961 gcggcggcac ggatcgatca tttactggct cttctggagc atcccggtcg catcgcgaac
     1021 gcaccgccgg ccattcatgg ctgggcctcg aatttcacgc cggtcgaata tcacgccgcg
     1081 atcgaagatg tgcaggcgca tattttcgcc ggcgatatct atcaggccaa tatagcgcag
     1141 cggttttccg caaagctgcc ggaggatttc gcgccgttcg gattttacga gaagctgcgc
     1201 gcggccaatc ccgcgccttt cgccgcttat ctcaatctag gcgagacggt catcgcgtct
     1261 tcgtcgccgg aattgttgct gcgcaagcgc ggtcgcggca tcgagacgcg gccgatcaaa
     1321 ggaacaatca atcgctcgcc cgatccggca aaagatgcac gccgcgcgca ggcgctgctt
     1381 aaatcggaaa aggatcgcgc cgaaaacatc atgatcgtcg atctgctgcg taacgatctt
     1441 tcgcgcgtca gcgagccaaa gtcggttgag gtgccggtgc tctgcggcct agaatcctac
     1501 gcaaacgtcc atcatctcgt ttctgtggtc acgggcgagc tggcacagag tagggatatc
     1561 gtcgatctga ttgccgcgac cttccccggc ggttcggtta cgggtacgcc caagcggcgc
     1621 gcgatggaaa tcatcgacgc gatcgaaaag gtctcacgcg gctattattg cgggtcgatc
     1681 ctctatatcg gtttcgacgg cacgatagat atgaacattg cgatccgaac cgcggtgctg
     1741 aaacccggcc gcgcgtcgct gcacgcgggc ggcggcatca caagcctgtc cgatccggtc
     1801 gcggaatatg ccgagaccga gattaaggtg aagcgtctgt tcgaggcgtt cggtgcggcc
     1861 gaggaattgg aagaggcggc gcaatgagca atcgcgtcct ggtcatcgac aattacgatt
     1921 ccttcgtctt caacgtcgcc cgctatttcg aagagcttgg acgcgatgtc gtcgtgcgcc
     1981 gcaatgatga aattgatgtc gccgagatcc gccgtgccgc accagccgcc attgtgattt
     2041 caccaggacc gggaacgccg cgcgatgccg gcatttctga ggccgtcgtt gaagaattga
     2101 ctggcgagat tccgatcctc ggcgtttgtc ttggccatca ggcgatcggc gaagtcttcg
     2161 gcggtagaat agatccagcc aagcgcccgc tttatggccg cacatcgcgg ctgacacata
     2221 gcggcaaagg tctttttgaa ggattgccgc agcctttggt cgtagggcgc tatcactcgc
     2281 tggttgttga acgcacgcca gcactcgaaa ccaacctttc gattgatgct gtgtccgagg
     2341 aaggcgagat tatggcgctc tcgcatcaca ctgcggcggt ttatggcgtc cagtttcatc
     2401 cggaatcaat tttgaccgaa tttggccacg ctcttttcgc taatttcttg cgtctcgcaa
     2461 tggaaagaaa gccgcgtgtt ctggtttaat ggtgcgatac aagagcagcc ggtcgtttcc
     2521 ttcgatttga cggatcgcgg actcaacctt ggcgacggcg tgttcgatac atcgctggcg
     2581 cgtaacggcc acatttttcg ccgcaagtcg catatcgatc ggatggcgga cgcggccgag
     2641 attcttggca ttcctttcga cgaaatggcc gccgggacgg ccctcgatgc gctcgccgag
     2701 gctatcggcg acggcgcggt tcggttgaca ctgacgcgcg ggccagggcc acgcgggcta
     2761 tcattcccga aaaatcccgc gccgcttctc tatggcgcgg ccgcagcgat gcacatgccg
     2821 ctgttcccgg ttttgaaact tggtacgaca tcgatccgcc gcaacgagac gtcgccggcc
     2881 tcgcgcatca aagcgttgcc ttatctcgat tccgtgctgg cgctggaaca ggcttccgcg
     2941 cgcggcgccg acgaggtttt gttcgagaat acgcatagcc gcgtggcttg cctcgcaacc
     3001 gcgaatatct ttgccatctt cggccgtcag ctcgtaaccc cgccgctgag cgacggcgcg
     3061 ctcgccggaa ccacacgcgc tttcgtcctt tccaaggcgc acggtttgca cttcgatgtc
     3121 gccgagcgtt cgctgccgtt ggagaaattc tcccgtgccg atgccctgtt tgcgaccagc
     3181 agcctgcggc tgatcgcccc gtgcagccag ctcgatgcca tcacctatcc ctccgaggcg
     3241 aacgagatcg tgatggaatt gcaggcctcg atcaaagagg tgattgcgag cgaatgcgga
     3301 cgcttttaaa ccgggcgtcc cgagcgcggc tttgcaaggc gcccgccacg ggagataata
     3361 gcgccgccgg gttcccggtt cgtccttact cctgcccggt ttcctcatgc tgcgcctagc
     3421 aattttgcgt catgccgaag cagtgccgct cacagacgaa tcggatgcgg atcgggaatt
     3481 gacccccgcg ggctgggaaa tggccgagcg gatgggccgc tatttccgcg atgcgggatt
     3541 gcggccggac cttgctttgg tctcacccgc aaagcgcgcc aaagaaactt tccgcggtct
     3601 cgaacggggc gcgggtgtga cgttcacggt agatttcgtc ggcaagctct acagcgccga
     3661 tctccgcact ttggaggagg tg
//
LOCUS       DASWOG010000091         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_148669,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000091 DASWOG010000000
VERSION     DASWOG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148669"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VGB93_01850"
     CDS             complement(<1..908)
                     /locus_tag="VGB93_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0144905.1"
                     /translation="MKSEKAGDAPMHGLASLPVFFKLAGRRVLVAGGGAGAAWKAELL
                     QATGAHVAVVSGAPGEDMRALAARTSALTLVTREWAESDFVDAALAIGDFATEDEAAC
                     FRDAARRARVPINVVDKPNYCDFQFGTIIDRSPLVVAISTAGAAPIFALALRGRIEAL
                     LPHSLKAWAQAAQTWRPKLGDAESRRRFWEYFVTRALGSRAPEPHDFDELIEAARRET
                     ETQKNAIACLAITSDDPELLTLKALRLLQSGEVVFYDAGITPQIVEMARRESARIRCD
                     AHDDIAAQILAEVRKGKRVVWLAADEL"
     gene            1006..1797
                     /locus_tag="VGB93_01855"
     CDS             1006..1797
                     /locus_tag="VGB93_01855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0144906.1"
                     /translation="MLIDLTGKSAIVTGSTRGIGFAAAKGLALSGARVVVNGRGQKAV
                     DEALSELKKAAPKAELQGVVADLGTVAGCDTLIKAVPSVDILVNNVGIFNPQDFFEIP
                     DSEWQRFFEVNVLSGVRLTRAYLPGMIARNWGRVIFISSESGLNIPVEMIHYGFTKTA
                     QISIARGIAKRAAGTGVTVNSVLPGPTLSEGVVDFLEKMSSDGGSVEETAAAFVKKHR
                     PSSIIQRAASVEEVANLIVYVASPQASATTGAALRVDGGVVDTIA"
     gene            complement(1810..2166)
                     /gene="queD"
                     /locus_tag="VGB93_01860"
     CDS             complement(1810..2166)
                     /gene="queD"
                     /locus_tag="VGB93_01860"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY0144907.1"
                     /translation="MKITQAFSFEAAHFLPKVAPTHRCRNLHGHSYRVELRLEGPVDA
                     ESGFVVDFFAIEKVFAPLHAQLDHHCLNEIPGLENPTAENIAIWIYVQIKPTFPELSA
                     VIVFETKDCCAEYEGH"
     gene            complement(2179..2388)
                     /locus_tag="VGB93_01865"
                     /pseudo
     CDS             complement(2179..2388)
                     /locus_tag="VGB93_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913632.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
BASE COUNT          458 a          702 c          762 g          466 t
ORIGIN      
        1 agttcgtctg cggcgagcca gacgacacgt ttgcctttgc gtacttcggc aagaatttgc
       61 gcggcaatgt cgtcgtgcgc gtcgcagcga attctggcgg attcgcgccg cgccatttcg
      121 acgatttgcg gcgtgatgcc ggcatcgtaa aaaacgactt ctccggattg gagaagcctt
      181 agcgctttca gcgtcagaag ctccggatcg tcggaggtga ttgcgagaca ggcgattgcg
      241 ttcttttgcg tctccgtttc gcgccgggcc gcttcgatca attcgtcaaa atcatgcggc
      301 tcgggcgcgc gcgaacccaa tgcgcgcgtc acgaaatact cccagaagcg gcgccgcgat
      361 tcggcatcgc cgagcttagg ccgccatgtc tgcgcggcct gcgcccaggc tttgaggctg
      421 tgcggtagca gcgcttcgat gcggccgcgt aatgccagcg cgaaaatcgg cgccgcgcca
      481 gccgttgaaa ttgcgacgac gagcggcgat cgatcgatga tcgtgccgaa ttggaaatcg
      541 caatagttcg gcttgtcgac gacattgatg ggcacgcgcg cgcgccgggc cgcgtcacga
      601 aaacacgcag cttcgtcttc tgtcgcgaaa tcgccgatgg ccaatgcggc atccacgaaa
      661 tcgctctcgg cccactcgcg cgttacaagc gttagcgccg aagttcgtgc cgccagagcc
      721 cgcatgtctt cgcccggcgc gccgctgaca acggcaacat gcgcgcccgt ggcttgcaac
      781 agctcggcct tccaggccgc gccagcgccg ccgcctgcga cgagaacgcg gcgcccggca
      841 agcttaaaga aaaccggcag gctggccagt ccgtgcatcg gcgcatctcc ggctttttcg
      901 gacttcacag gcacgcctcg aacaatttgt ggtcatcctc tatagcgcag cgcgaaggga
      961 gggcctaaat agggctcgaa acccaacgtc cgacggagaa gagaattgtt gatcgaccta
     1021 actggcaaga gcgcgattgt gaccggctcg acgcgcggca tcggcttcgc cgccgccaag
     1081 ggcttggccc tctccggggc gcgtgtcgtc gtcaacgggc ggggccagaa ggcggtggac
     1141 gaggctttga gcgaattgaa aaaggccgcg ccgaaggctg agcttcaagg cgttgtggcc
     1201 gatcttggca ccgtcgcggg ctgcgacacg ctcatcaagg ccgtcccgtc ggtggatatt
     1261 ctcgtcaaca atgtgggtat cttcaatcca caggatttct ttgaaattcc tgattccgaa
     1321 tggcagcgct ttttcgaggt gaatgtgctg tcgggcgtgc ggctgacgcg ggcctatctg
     1381 ccgggcatga tcgcgcgcaa ttgggggcgc gtgatcttca tctcctcgga atctggcctg
     1441 aacattcccg tcgagatgat ccattacggc tttaccaaga cggcgcagat ttcgattgcg
     1501 cgcggcatcg ccaagcgggc cgccggcacg ggtgtaacag tgaattccgt gctgccggga
     1561 ccgaccctgt cggaaggcgt cgtcgatttc ctcgaaaaga tgtcgagcga cggcggatcg
     1621 gtcgaagaga cagcggcggc ctttgtcaaa aagcatcggc cgagttcgat catccagcgc
     1681 gcggccagcg tcgaggaagt cgccaatctg atcgtctatg tcgcttcgcc gcaagcctcg
     1741 gcgacgaccg gcgcggcgct ccgcgtcgat ggcggcgtgg tcgatacaat tgcctaaaag
     1801 aatgaggggc tagtgcccct catattccgc gcagcaatcc ttcgtctcaa agacgatgac
     1861 ggcggagagt tcggggaatg tcggcttgat ctgcacgtaa atccagatcg cgatattttc
     1921 cgcggtcgga ttttcgaggc cggggatctc gttcaggcaa tgatgatcga gttgcgcgtg
     1981 cagcggcgcg aagacttttt cgatggcgaa gaaatcgacg acgaagccgc tctcggcatc
     2041 aaccggccct tcgagccgga gttcgacgcg ataggaatgg ccgtgcaggt tgcggcagcg
     2101 atgcgtcggc gcgaccttgg gcaggaaatg cgccgcctcg aatgaaaaag cctgggtgat
     2161 cttcatctgc tcaatttgtt agggcaagcc gatcagcttg tgagtttgca ggctcaggcg
     2221 ccagcgcgga tgcgccaggc aataggccgc cgcctgctgg atgttggcgc cctgcgccgg
     2281 cccgtccatc ggttgcagga agaaatgccg gaaggcaagc cccgcgaagc tttccggatc
     2341 gaggccgtcc tgcgggaaaa ccagcttcaa ttagtcgccg gatttgag
//
LOCUS       DASWOG010000092         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_240388,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000092 DASWOG010000000
VERSION     DASWOG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240388"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGB93_01870"
     CDS             complement(<1..246)
                     /locus_tag="VGB93_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY0144908.1"
                     /translation="MRRVWYQRRQTPAQQQDSTPPKRSLINIIGPGFITGAADDDPSG
                     IATYSQTGAQFGFGQLWTALYQVPLLLAVQECCGRIGA"
     gene            complement(264..1085)
                     /locus_tag="VGB93_01875"
     CDS             complement(264..1085)
                     /locus_tag="VGB93_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0144909.1"
                     /translation="MKISLIQMNSRQDKAANIATACELIEQAVVDERPDWVALPECFD
                     FLGGQRADKQQAAEVLGDGPAYLAMQALARRHGIFIHAGSILEKIPGEDRLHNTTVVF
                     DRAGREIAIYRKIHMFDVVTPDGVQYRESASFKPGTEIVIYQAEGFTFGCAICYDLRF
                     PDLFQALAEKGADVIALPSAFTLLTGKDHWEVLCRARAIETETYFCAPAQTGAHVVGN
                     ETRATYGHSLVVDPWGHVIARASDGVGSVSTRIERPRIDKVRAQIPVAQHKIKLR"
     gene            complement(1146..>2196)
                     /locus_tag="VGB93_01880"
     CDS             complement(1146..>2196)
                     /locus_tag="VGB93_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0144910.1"
                     /translation="RPVDILSRQMSSLSGGDVDIEIGGTARKDEIGVISRSLQVLRDG
                     VKRNNELVDELHARDDREKRLMREAAVRAKVEKFSADLSANVARLTEMTKRMSTASQD
                     MILGARNAAEGSSQAKTASSNAAGDVSSVAVASEQLLASIEEISRQVVQSTTVVKRAV
                     SESIETSSGMARLTEAARRVGDIVSLISRIAAQTNLLALNATIEAARAGEAGRGFAVV
                     AQEVKTLATQTARATQDISGQIADMQAATESSVTAIDTIQTKIGEIEQISAIIASAVQ
                     EQGASTQEIARNVRSAASGTTATSAYVENVAQAAALTSQHAESLVGLAGELDTLAAHV
                     LSDVRAFGDALREAA"
BASE COUNT          408 a          673 c          694 g          421 t
ORIGIN      
        1 cgcgccgatg cggccgcaac attcctgaac cgcgagcagg agcggcactt gatagagcgc
       61 cgtccacaat tggccgaagc cgaattgcgc gccggtctgc gaataggtcg cgataccgga
      121 gggatcgtca tcggccgcgc cggtgatgaa cccggggccg atgatattga tgagcgagcg
      181 ctttggtggt gtggagtctt gctgctgcgc cggggtctgt ctgcgttgat accaaacacg
      241 gcgcatggat gctttcctcg tatctatcgc aatttgattt tatgttgggc gacgggaatt
      301 tgcgcacgca ctttatcgat gcgcgggcgc tctatccgcg tcgagacgct tccgacgcca
      361 tccgaagccc gggcgatcac atgcccccac ggatcgacga caagcgagtg cccataagtc
      421 gcgcgtgtct cgttgcccac gacatgcgcg cccgtctgcg ccggagcgca aaaataagtt
      481 tccgtctcga tggcgcgcgc gcggcataga acctcccaat ggtccttgcc ggtaagcagc
      541 gtgaaagcgg agggcagcgc gatgacatcg gcgccttttt ccgccaaggc ctggaacagg
      601 tccggaaagc gcaagtcgta gcagatggcg cagccgaacg tgaaaccttc ggcctgatag
      661 atcacgatct cagtgcctgg tttgaacgat gcgctttcgc gatattgcac gccgtccggc
      721 gtcacgacgt cgaacatatg gatcttgcga taaatggcga tctcgcggcc agcgcgatcg
      781 aagaccacag tggtgttatg aaggcgatcc tcaccaggaa tcttttcgag gatcgagccg
      841 gcatgaataa agatgccgtg ccggcgcgcc agcgcctgca tggcgagata ggccggccca
      901 tcgccgagaa cttccgcggc ctgctgcttg tcggcgcgct ggccgccgag aaaatcgaaa
      961 cattccggca gggcgaccca atccggccgt tcgtccacga cagcctgttc gatcaactcg
     1021 caggccgtag cgatattcgc cgccttatcc tggcgcgaat tcatctggat cagactgatc
     1081 ttcatatgcc tagttcgcgg gcttcgcgga ccaagtcaaa tgaaaatccg ggggatgaaa
     1141 ccctgtcagg ccgcctcgcg caacgcgtcg ccgaaggcac gcacgtcgga gagaacgtgg
     1201 gccgccagtg tgtcgagttc gcccgcgaga ccgacgagcg attcggcatg ttggctggtg
     1261 agcgccgcgg cttgggcgac attctcgaca taagcagacg tcgcggtcgt accggaggcg
     1321 gcagagcgca cgttgcgggc aatctcctgg gtcgaagcgc cctgctcctg caccgccgac
     1381 gcgatgattg cggagatctg ttcgatctcg ccaatcttgg tctgaatcgt atcgatcgcg
     1441 gtcaccgagc tctcggttgc cgcctgcata tcggcgatct ggccggaaat atcctgcgtc
     1501 gcgcgcgcgg tctgcgtggc gagcgtcttg acctcctgcg cgaccaccgc gaagccgcgg
     1561 ccggcctcgc cggcacgggc ggcttcgatt gtggcgttga gggccagaag attggtctgt
     1621 gccgcgatcc ttgagataag gctgacaata tcgcccacgc gacgggctgc ttcggtcagg
     1681 cgcgccatgc cgctcgacgt ctcgatgctt tccgagacgg cgcgtttgac gacggtggtc
     1741 gattgcacga cctgacggct gatctcttcg atcgaggcca acaattgctc cgaggcgact
     1801 gcgaccgagg agacgtcgcc ggcggcattg gaggaagccg tcttggcctg cgacgagccc
     1861 tcggcggcgt tgcgggcgcc caagatcatg tcctgagagg ctgtcgacat gcgcttggtc
     1921 atttccgtca gccgcgctac attcgctgaa agatcggcgg agaacttctc gactttggcg
     1981 cggacggccg cctcgcgcat gagccgcttc tcacggtcgt cacgggcgtg gagctcatcc
     2041 accaactcgt tgtttctctt cacgccatcg cggagcacct gaagggaccg cgagatgacg
     2101 ccgatctcgt ccttgcgcgc agtgccaccg atctcgatat caacgtcgcc acccgagagc
     2161 gacgacatct ggcgagagag aatgtcaacc ggccgg
//
LOCUS       DASWOG010000093         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_167096,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000093 DASWOG010000000
VERSION     DASWOG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167096"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="rpsN"
                     /locus_tag="VGB93_01885"
     CDS             complement(<1..277)
                     /gene="rpsN"
                     /locus_tag="VGB93_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY0144911.1"
                     /translation="MAKKSSIEKNKHREKLVKQYAARRKRLKAVADDQNLTAEERFAA
                     RLKLAELPRNSSAVRVRNRCEVSGRPRGFIGKMKMSRIAVRDLGSSGQ"
     gene            complement(314..997)
                     /gene="rplE"
                     /locus_tag="VGB93_01890"
     CDS             complement(314..997)
                     /gene="rplE"
                     /locus_tag="VGB93_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0144912.1"
                     /translation="MAEPKNEKGAPKGGAKKDAKPAKGGGETRSARARENASAVPFIA
                     PADYVPRLKKHYNEVVRDELVKQFGYKNALEVPVIDKIVINMGVGEAVNDTKKVTSAA
                     ADLALIAGQKPVITRARKAIATFKVRENMPIGAKVTLRKTRMYEFLDRLVTIALPRVR
                     DFRGLNPKSFDGRGNYALGIKEHLVFPEIDYDKAESMLGLDVIVCTTAKTDEEARALL
                     RAFNFPFRQ"
     gene            complement(990..1307)
                     /gene="rplX"
                     /locus_tag="VGB93_01895"
     CDS             complement(990..1307)
                     /gene="rplX"
                     /locus_tag="VGB93_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0144913.1"
                     /translation="MAAKIKKGDKVVVLTGRDKGRAGEVLQVLPRDERAVVRGVNIVK
                     RHQRQTPSQEGGIISKEATIHLSNLALADPKDGKATRVSFKILDDGRKVRVAKRSGDL
                     IDG"
     gene            complement(1307..1675)
                     /gene="rplN"
                     /locus_tag="VGB93_01900"
     CDS             complement(1307..1675)
                     /gene="rplN"
                     /locus_tag="VGB93_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY0144914.1"
                     /translation="MIQMQTNLDVADNSGARRVMCIKVLGGSKRKYASIGDIIVVSIK
                     EAIPRGRVKKGDVMKAVVVRTAKDIKRQDGSVIRFDSNAAVLINNQSEPVGTRIFGPV
                     PRELRAKNHMKIISLAPEVL"
     gene            complement(1767..2018)
                     /gene="rpsQ"
                     /locus_tag="VGB93_01905"
     CDS             complement(1767..2018)
                     /gene="rpsQ"
                     /locus_tag="VGB93_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY0144915.1"
                     /translation="MPKRVLQGVVVSDKQDKTVVVKVERRFTHPLLKKTVRRSKNFHA
                     HDEANKAKVGDTVLIEETRPISRLKRWVIVEVASATTTN"
     gene            complement(2022..>2140)
                     /gene="rpmC"
                     /locus_tag="VGB93_01910"
     CDS             complement(2022..>2140)
                     /gene="rpmC"
                     /locus_tag="VGB93_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0144916.1"
                     /translation="LRFQRATGQLQDTARVRIVRRDIARAKTIAATQAKKKV"
BASE COUNT          383 a          686 c          590 g          481 t
ORIGIN      
        1 tctggccact ggagccgaga tcacggactg caatacgcga catcttcatc ttgccgatga
       61 agccgcgcgg acggccggag acttcgcaac gattgcgcac gcgcaccgcc gacgaattgc
      121 gcggcaattc ggcaagcttc aaccgcgctg caaagcgctc ctcagcggtg aggttctggt
      181 catctgccac agccttcagc cgcttacggc gcgccgcata ttgcttgacg agcttctcgc
      241 gatgcttgtt cttttcgatc gaacttttct tagccatata cctgcctctt ggctccgcgt
      301 ctgagaccgg gtctcactgc cggaacggga agttgaatgc gcgcaaaagg gcgcgcgcct
      361 cttcatcggt tttcgccgtc gtgcaaacga taacgtcgag accaagcata ctctcagcct
      421 tgtcgtaatc gatttcgggg aacacgaggt gctccttgat gccaagcgca taattaccgc
      481 gcccgtcaaa gctcttcggg ttcaggccgc gaaaatcgcg tacacgcggc aacgcaatcg
      541 tcacgagacg atcgagaaac tcgtacatcc gcgtcttgcg caacgtcacc ttcgcgccga
      601 tcggcatgtt ctcgcgcacc ttgaaggtcg cgatcgcctt gcgggcccgg gtgatgacag
      661 gcttctggcc ggcgatcaga gcaagatcag ccgcggcaga cgtaaccttc ttcgtgtcgt
      721 tgaccgcctc gccgacgccc atattgatca cgatcttgtc gatcaccggt acttcaagcg
      781 cgttcttata gccgaactgc ttgacgagct cgtcgcgcac gacctcgttg taatgcttct
      841 tgaggcgcgg cacgtaatcc gccggcgcaa taaatggcac ggcagaggcg ttctcacgcg
      901 cacgggccga gcgggtttcg ccgccgccct tggccggctt tgcgtctttc ttggcgccgc
      961 ccttcggcgc gcctttttcg tttttgggct cagccatcga tcagatctcc cgaacgtttg
     1021 gcaacgcgga ccttgcggcc gtcgtcgaga attttgaagc tcacccgcgt tgccttgccg
     1081 tccttcggat cggccaacgc gaggttcgag aggtggatcg tcgcctcttt cgaaatgatg
     1141 ccgccttcct ggctcggcgt ctggcgttga tggcgcttga cgatattgac gccacgcaca
     1201 acagcacgct catcacgcgg caatacctgc agcacctccc cggcgcggcc cttatcgcgg
     1261 cccgtgagaa cgacgacttt gtcgcccttc ttaatcttcg cggccatcac agaacctccg
     1321 gcgcgagcga aataatcttc atgtggttct tggcgcgcaa ttcgcgcggc accggtccga
     1381 agatgcgggt gccaaccggc tccgactgat tgttgatcag tacagccgca ttggagtcga
     1441 agcggatgac cgagccgtcc tgccgcttga tgtccttggc ggtccgcaca acgaccgcct
     1501 tcatcacgtc gcccttcttg acgcggccgc gcggaatcgc ctccttgatc gaaacgacga
     1561 taatgtcgcc gatcgaggca tacttccgct tagaaccgcc gagcaccttg atgcacatca
     1621 cgcggcgcgc gccggaattg tcggcgacgt cgagattagt ctgcatctgt atcatggcct
     1681 tgcgcctttc ttatcagaca ggtttccgtt gccggacttc gcctgagttt cctcgttcca
     1741 atgccaaatc cctcgcggga cttggcttaa ttcgttgtgg tcgccgaggc aacctcgaca
     1801 atgacccagc gtttaagccg cgagatcgga cgagtttcct caatcagcac cgtatcgccg
     1861 accttggcct tattggcctc gtcgtgcgcg tgaaagttct tcgaccgccg cacagtcttc
     1921 ttcagcagcg gatgggtgaa acgccgttcg accttcacca cgactgtctt atcctgcttg
     1981 tcgctgacga cgacgccctg gagaacgcgt ttcggcatga cttaaacctt tttcttagct
     2041 tgtgtggcgg cgatcgtctt cgcccgcgcg atatcgcggc ggacgatgcg cacccgcgcc
     2101 gtatcctgaa gctgcccggt cgcccgctga aagcgcagat
//
LOCUS       DASWOG010000094         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_240390,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000094 DASWOG010000000
VERSION     DASWOG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240390"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..591)
                     /locus_tag="VGB93_01915"
     CDS             complement(142..591)
                     /locus_tag="VGB93_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0144917.1"
                     /translation="MSKSLAAQEIANCICLRLRKTARRVTQIYDQMLEPAGLTLTQFS
                     LLAHLYAGEEFSVGDLAEALDTDPTTLTRNLKPLIERQYVNVRQDEDDRRRRAIVLTP
                     AGRDLLPVAYPLWRKAQAHMARLLGESQAAQLNKTLDRSLGRLTRAV"
     gene            741..>1728
                     /locus_tag="VGB93_01920"
     CDS             741..>1728
                     /locus_tag="VGB93_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY0144918.1"
                     /translation="MVTFAEHPTVQTMRARAAAGASPEVPSVIDAAWLRNLCLEMGAD
                     DVRFVAIESPDIVGQKSEIFGAFPATKAMISFVCRMNRENIRTPARSIANVEFHRSGD
                     EINDTARHIVSALQDLGIGARNGAPVGFPMEADRWGADKMWIVSHKPVAVAAGLGRMG
                     IHRNVIHPKFGNFILLGTILIDTEISAYGRPLDYNPCLSCKLCVAACPTGAISPDGAF
                     NFQACYTHNYREFMGGFGDWVETMTDAHSAKDYRRKVSDSETVSMWQSLSFGANYKAA
                     YCLSVCPAGEDVIGPFLADRIGFNKAIVEPLRAKQETVYVAQNSDAETYVPRH"
BASE COUNT          342 a          522 c          498 g          366 t
ORIGIN      
        1 cgctcgggcg caattcgtat cgtaccgaca atcaggagca tgcgggccgc cgtgctgata
       61 agttaacgct gcaacaagcc gcaaaacccg agccgcgtca acgctgtttg cgtccggcgg
      121 cctagcacgc gccgcgaata atcagaccgc gcgagtcaat ctgccgagcg accgatcgag
      181 tgttttattg agctgggcgg cctggctctc gcccagcaga cgcgccatat gtgcctgggc
      241 cttgcgccac aaaggatagg cgacaggcag caggtcccgc cccgccggcg tgagcacaat
      301 tgcgcggcgg cggcgatcgt cttcgtcctg acgaacgttc acatactgac gctcgataag
      361 cggtttgaga ttacgcgtca gcgtcgttgg gtcggtgtcc aaggcctccg ccaaatcgcc
      421 gaccgagaat tcctcccccg cgtagagatg cgcgagcagg ctgaactgcg tgagcgtcag
      481 cccagctggc tccagcattt ggtcatatat ttgcgtgacg cggcgcgccg tcttgcgcaa
      541 gcgcaagcag atgcaattgg cgatttcctg ggcggctagc gatttgctca taatccgttt
      601 tgattttacc gctcttcttg gagcttgaat atatcactgc atatacagcg atgccaatac
      661 tgcgccgtgc acagggttca cgccttgaca cgggaccgat attatctgta tgtacagata
      721 atattatctg tggaggggca atggtaacgt tcgctgaaca tccgaccgtg cagacgatgc
      781 gcgcccgcgc cgcggccggc gcctcgcctg aggtacccag cgttatcgac gccgcatggc
      841 tcagaaacct gtgcctagaa atgggtgccg acgatgtccg ctttgtcgcg atcgagagcc
      901 cggacatcgt cgggcagaaa tcggaaatct ttggcgcttt ccctgcgacg aaagcgatga
      961 tcagttttgt ctgccggatg aaccgcgaga atatccgcac acccgcgcgc tccatcgcca
     1021 atgtcgagtt ccatcgcagc ggcgatgaga tcaatgacac tgcgcgtcat attgtttcgg
     1081 cgcttcagga tttaggtatc ggcgcgcgca acggcgcgcc agtcggcttt ccgatggagg
     1141 ccgaccgctg gggcgcggat aaaatgtgga ttgtctcgca caaacccgtt gcggtcgcgg
     1201 cggggctcgg ccgcatgggc atccaccgca atgtcattca tcccaaattc ggcaatttca
     1261 ttctgctcgg taccattctc atcgacacgg agatttcggc ctacgggcgg ccgctcgatt
     1321 acaatccgtg cctctcgtgc aaactctgtg ttgcggcctg cccgaccggc gccatcagcc
     1381 ccgacggcgc cttcaatttc caggcttgtt acacgcacaa ttatcgcgaa ttcatgggcg
     1441 gcttcggcga ttgggtcgag acgatgaccg atgcgcacag cgccaaagat tatcgccgca
     1501 aggtttcgga ttccgaaacc gtctcgatgt ggcagagtct ctcctttggg gccaattata
     1561 aagccgccta ttgcctctcc gtctgcccgg cgggcgaaga cgtcatcgga cctttccttg
     1621 ctgaccgcat cggcttcaat aaagcgatcg tcgagccgct gcgcgccaag caggagacgg
     1681 tctacgtggc gcaaaattcc gacgccgaga cctatgttcc gcgtcatt
//
LOCUS       DASWOG010000095         5448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_350391,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000095 DASWOG010000000
VERSION     DASWOG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350391"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..657)
                     /locus_tag="VGB93_01925"
     CDS             complement(52..657)
                     /locus_tag="VGB93_01925"
                     /inference="COORDINATES: protein motif:HMM:NF044584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144919.1"
                     /translation="MAEDERKRKASLKTARRAAVKKRAATKKKPASKKPILKKSIAKK
                     AAAKRAKSTAKSPAAKKIPARKSVPIKKARVSKRAFLPPLPPPASARKTPRSPARSPS
                     RAKALGRAKTGSISKTVCEIPACTEGVLRQKLRLAAPYVELWLAQKVSTPEDETTPEA
                     AFVRVWQWLMLAAALEGSHVVITPDLVQKLFAEEMGRAPTA"
     gene            complement(682..2013)
                     /gene="glcF"
                     /locus_tag="VGB93_01930"
     CDS             complement(682..2013)
                     /gene="glcF"
                     /locus_tag="VGB93_01930"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY0144920.1"
                     /translation="MQTIFTDAQLEDSHMAVSEKILRSCVHCGFCTATCPTYVLEGDE
                     LDSPRGRIYLIKEMFEQGRAAPADIVTHVDRCLSCLSCMTTCPSGVHYMHLVDHARAH
                     IEETYKRPMTDRGMRSLLAAVLPYPRRFALALRGAALLRPLTEILKRIAGAPQKTPRL
                     IDRLVAMLALAPGKLPRSAAPPAMSPAQGEKRGRVALLKGCAQQVLNPRINEATVRLL
                     TRNGIEVIQGPDEVCCGALVHHMGKTPEALDFARANVDAWMKVKAAGGLDAILVTASG
                     CGTTIKDYGFMLREDPAYAEKAAEVSRLAKDICEYAAGLDIGAMAQPQELTVAYHSAC
                     SLQHGQKITDQPKRLLKAAGFRLRDVPEGHICCGSAGTYNIMQPYFAEQLRERKIGNI
                     EAVRPDIIAVGNIGCMMQIASGTDIPVVHTVELLDWANGGPMPLQIERTGA"
     gene            complement(2017..3219)
                     /locus_tag="VGB93_01935"
     CDS             complement(2017..3219)
                     /locus_tag="VGB93_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0144921.1"
                     /translation="MNFVAPKTEAETVEAVNAVRSARQKIAIRGGGTRAGLGRPVEAE
                     TQLSTRGLTGITLYEPSELVISARAGTPLSEIETALQEKNQTLPFEPMDHRALYGTSG
                     EPTIGGIAACNISGPRRISAGAARDLLIGLRFVNGRGEIVKTGGRVMKNVTGLDLVKL
                     LCGGHGTLGIITEATFKVLPHPAFAGTLALYGLADGEAVAALCAAQGTPLSISAAAHL
                     PAGLATPEPLTLFRLEGPQASVDERLSALRRTLAPFESGEALAPDYAGALWRAICNVV
                     PLVSPPDAAIWRVSIQPTRAPKFVDLVLRQLPARYYYDWGGGLVWLSIAAAGDAGGAI
                     IRGALKEAGNGQTIGHGILVRAPEDVRSRVNVFEPLSLPLHKLTKDIKSSFDPDGILN
                     PGRMYAGI"
     gene            complement(3261..4697)
                     /locus_tag="VGB93_01940"
     CDS             complement(3261..4697)
                     /locus_tag="VGB93_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0144922.1"
                     /translation="MTGIQFPKLDASILARREAIIEGLKLVVAPESLITTEDERRAFE
                     TDALTAYRRMPLAVVLPRSTEELSADMAYLHQEGVNIVARGAGTSLAGGAIPQEDAVV
                     IGVAKMARILDIDYANRTIRVQSGVTNLSISNAVMAERFFYAPDPSSQLACTIAGNIG
                     MNSGGAHCLKYGVTTNNVLGVKMVLVDGTVVEIGGPYLDSGGLDLLGLVVGSEGQLGI
                     VTEATLRILPMAEDARPVLFGFPTSEAAGGCVAAIIGAGIIPVAIEFMDKLAVEICED
                     FAHAGYPLDVDAMLIIESEGSPAEIDAELAKIVAIAKDYGVSVVKESKSAMETAAIWK
                     GRKSAFGATGRVADYICMDGTIPTGKLPQVLHRLNEIVADLGLRVANVFHAGDGNLHP
                     LVLYNINDPVEQAKAEKAGEEILKLCVEVGGCLTGEHGVGIEKRDLMPYQFSPVDLAQ
                     QMRVRAAFDAGWRLNPAKVFPLEGRAAA"
     gene            complement(4745..5209)
                     /locus_tag="VGB93_01945"
     CDS             complement(4745..5209)
                     /locus_tag="VGB93_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY0144923.1"
                     /translation="MGQEIEHKFRVDVSRWRPQGEGVAIRQGYLSAEKDRVVRIRTAG
                     PKAYLTVKGPGHISRAEFEYEIPFEDAAAMLNDLCLPGAIEKTRYKQDVGGHVWEIDV
                     FHGDNEGLVVAEIELGSEDETFEQPDWVLDDVSRDPRYFNASLSKAPFRTWT"
     gene            5258..>5448
                     /locus_tag="VGB93_01950"
     CDS             5258..>5448
                     /locus_tag="VGB93_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0144924.1"
                     /translation="MEVPQRPRVGLFVTCLVDLFRPSIGWATVKLLEDAGCRVEIPVQ
                     TCCGQPDYNSGDRRLAKDLA"
BASE COUNT         1013 a         1702 c         1693 g         1040 t
ORIGIN      
        1 tagaggttgg acggagtccg atcgcagcta tggcgtcgtc ccgcttgggc gttacgcggt
       61 gggagcgcgt cccatttctt ccgcgaagag cttttggacg agatccggcg tgatcacgac
      121 atggctgcct tcgagcgctg ctgcgagcat gagccattgc caaacgcgga cgaaggcggc
      181 ttccggcgtc gtttcgtcct cgggcgtact caccttttgc gcgagccaca gttcgacgta
      241 aggcgcggca agccgcagct tctgccgcaa aacgccctcg gtgcaggccg gaatttcaca
      301 aacagtcttg ctgatgctgc cggttttagc gcggccaagc gccttggcgc gtgatggcga
      361 ccgcgccggg ctgcgtgggg ttttgcgggc cgaagccggc ggcggcaaag gcggcagaaa
      421 cgcgcgcttg ctcacgcgcg ctttcttgat tggtacggat ttccgcgccg gaattttctt
      481 agccgcgggg gacttggcgg tagattttgc gcgtttggca gcggctttct tcgcgatgga
      541 ttttttcaat atcggcttct tggacgccgg ctttttcttg gtcgcggcgc gctttttcac
      601 cgccgcacgc cgcgcggtct tcagcgacgc ttttcgcttg cgctcatcct ccgccatgcc
      661 cgtctccagc cgagagcgac atcatgcgcc ggtgcgttcg atttgcaaag gcatcggacc
      721 gccgttcgcc caatccagca actcgactgt atggaccaca ggaatatcag taccgctcgc
      781 aatctgcatc atgcagccga tgttgccgac ggcgatgatg tcgggccgca cggcttcgat
      841 attgccgatt ttacgttcgc gaagctgctc ggcaaaatag ggctgcatga tgttataagt
      901 gccggccgaa ccgcagcaga tatggccctc cggcacatcg cgcagacgaa agcccgcagc
      961 cttcagcagc cgcttcggct gatcggtgat tttctgccca tgctgcaacg agcacgcgga
     1021 atgataagca acggtcaact cttgcggctg cgccatcgcg ccgatatcga gcccggcggc
     1081 atattcgcaa atgtccttgg cgagccgcga aacctccgcc gccttttcgg cataggcggg
     1141 atcttcgcgc agcatgaaac cgtaatcctt gatggtcgtg ccgcagcccg acgcagtgac
     1201 gagaatggca tcgaggcccc ctgcggcttt gaccttcatc catgcatcga cattggcgcg
     1261 ggcgaaatcg agcgcctccg gcgtcttgcc catgtgatgc accagcgcgc cgcagcagac
     1321 ttcatccggc ccctggatga cttcgatgcc attgcgtgtg agcaagcgaa ccgtggcttc
     1381 attgatgcgc ggattgagaa cctgctgcgc gcagcctttg agaagtgcga cgcggccgcg
     1441 tttctcaccc tgcgccggag acatcgccgg cggagcggcg gagcgcggga gcttgcctgg
     1501 cgcgagggcc agcatggcga cgagccggtc gatcaggcgc ggagtcttct gcggcgcgcc
     1561 cgcgatgcgc ttcagaattt cggtcaaggg ccggagcagc gcggcgccac gcagtgccaa
     1621 tgcgaaacga cgcggataag gcagcacagc cgcgagcagg ctgcgcatcc cacgatccgt
     1681 catcggtcgt ttgtatgttt cctcgatatg ggcgcgcgca tgatcgacaa gatgcatgta
     1741 atgcacgccc gacgggcatg tcgtcatgca agacaggcag gataagcagc ggtcgacatg
     1801 cgtgacaatg tcggcaggcg cagccctgcc ctgctcgaac atttccttga tgaggtagat
     1861 gcgcccgcgc gggctgtcga gttcgtcgcc ttcgagcaca taggtcggac aggtggcggt
     1921 acagaagccg caatgaacgc acgagcgcag aatcttttcc gagacggcca tatggctgtc
     1981 ttcgagctgc gcgtcggtga aaattgtttg catagatcag atacccgcgt acatgcggcc
     2041 gggattgaga atgccgtccg gatcgaaact ggatttaatg tctttcgtca gtttgtgcag
     2101 cggcagcgaa agcggctcga agacattgac gcggctgcga acatcttccg gcgcgcggac
     2161 caaaatcccg tggccgattg tctggccgtt ccctgcctct ttcagcgcgc cgcgaatgat
     2221 tgctccgccg gcatcgcccg ccgctgcaat cgataaccag acgaggccgc cgccccaatc
     2281 gtaataatag cgcgccggaa gctgccgcaa gacgagatcg acgaatttgg gcgctcgcgt
     2341 cggctgaatg gagacacgcc agatggcagc atccggcggc gacacgagcg gcacgacatt
     2401 gcagatggcg cgccatagcg cgccggcata atcgggcgcc agagcttccc cggattcgaa
     2461 cggcgcgagg gttcgccgca acgccgagag gcgctcatcg acggaggctt gcggcccttc
     2521 gagacgaaac aatgtgagcg gctccggcgt cgcaagcccg gccggcagat gcgcagcggc
     2581 gcttatgctg agcggcgtgc cctgcgcggc gcagagtgcg gcaacggctt cgccatccgc
     2641 cagcccatag agcgctaatg tcccggcgaa cgcgggatgc ggcaatacct tgaaggtcgc
     2701 ttcggtgatg atcccaagcg tgccatggcc gccgcaaaga agtttcacga ggtcgagacc
     2761 ggtgacattc ttcatcactc ggccgccggt cttcacgatc tcgccgcgac cgttgacgaa
     2821 acgcaggccg atcagcaggt cacgcgccgc gccggccgaa atacgccgcg ggccggagat
     2881 gttgcaggcg gcaatgccgc cgatggtcgg ttcgccggat gtgccataga gagcgcgatg
     2941 atccatcggc tcgaacggga gagtctggtt tttctcttgc agcgccgttt cgatttcact
     3001 gagcggcgtg cccgcccgcg ccgagatgac aagctccgat ggctcgtaga gcgtgatgcc
     3061 ggtaaggccg cgcgtcgaaa gctgcgtttc ggcctcgacc ggcctcccaa gccccgcgcg
     3121 cgtgccgccg ccccggatgg caattttctg ccgagccgaa cggaccgcat tgacggcttc
     3181 gacagtctcg gcctcggtct tgggtgcgac gaaattcatg gcggcgtggg ctagcacgga
     3241 taaacgggcc tgtcgcccgt ctaagccgcc gctctcccct cgagcggaaa gaccttggcc
     3301 gggttgagcc gccagccggc atcgaaggcg gcgcgcacgc gcatctgctg cgccagatcg
     3361 accgggctga actgatacgg catcagatcg cgcttttcga tgccgacgcc atgttcgcca
     3421 gtgaggcagc cgccgacttc gacgcagagc ttgaggattt cttcgccggc tttctcggcc
     3481 ttcgcctgct cgaccggatc gttgatgttg tagagcacga gcggatgcag attgccatcg
     3541 ccggcatgga atacattggc gacgcgcagg ccaaggtccg cgacaatctc gttcagccgg
     3601 tgcagcacct gcggcagctt gccagtcgga attgtgccat ccatgcaaat gtaatccgcg
     3661 acgcggccgg tcgcgccgaa ggcagacttg cgccccttcc agatcgcagc cgtctccatc
     3721 gcggatttcg attccttgac gacgctcacg ccataatctt tggcgatggc aacgatcttg
     3781 gcgagttccg catcgatctc ggcaggcgaa ccttccgact cgatgatcag catcgcgtca
     3841 acatcaagcg gatagcccgc atgggcgaaa tcctcacaaa tctcgacggc cagcttgtcc
     3901 ataaattcga tcgcgacggg tatgatcccg gcgccgatga tcgccgcaac gcagccgcca
     3961 gcggcttcgc ttgtcggaaa gccgaacagc acaggtcgtg catcttccgc catcggcagg
     4021 atgcgcaagg tcgcttccgt cacaatgcca agctggcctt ccgagccgac aacaaggcca
     4081 agcagatcga ggccgccgct gtcgagataa gggccgccga tctcgaccac cgtaccgtcg
     4141 acaagcacca ttttgacgcc cagcacgtta ttggtcgtca cgccgtactt gagacaatgc
     4201 gcgccgccgg aattcatgcc gatgttgcca gcaatcgtgc aggcaagctg cgacgaggga
     4261 tcgggcgcgt aaaagaaacg ctcggccatc accgcgttcg agatcgacag attggtcacg
     4321 ccggattgca cacggatcgt ccggttggcg taatctatat cgaggatgcg ggccattttg
     4381 gcgacgccga tgacgacggc atcttcctgc gggatggcgc caccggcaag cgacgtgccg
     4441 gcgccgcgcg cgacgatatt gacgccctcc tgatgcagat aggccatgtc ggcggaaagc
     4501 tcttcggtcg agcgcggcag gacgacagcc agcggcatac gacgataggc ggtcaacgcg
     4561 tcggtctcga acgcgcgccg ctcatcttcc gtcgtgatca gcgactcggg cgcgacgacg
     4621 agcttaaggc cttcgatgat cgcctcgcgc cgggcgagga tcgaggcgtc gagcttggga
     4681 aattggatgc cagtcatgcg tgaacatgcg ccaaagaggc gctcggcggc aagctttttg
     4741 actttcaggt ccacgtcctg aagggcgcct tggacaggct ggcgttaaaa tagcgcggat
     4801 cgcgcgagac atcgtccagc acccagtccg gctgctcgaa tgtctcatct tcgctgccaa
     4861 gctcgatctc ggccacaacg aggccctcgt tgtcgccatg aaagacgtcg atctcccaga
     4921 catggccgcc aacgtcttgc ttatagcgcg tcttctcgat cgcccccggc aggcagaggt
     4981 cgttcagcat cgcggcggca tcttcgaatg gtatttcgta ttcgaattcc gcacggctga
     5041 tatgcccggg gccttttacg gtgagataag ctttcggtcc cgccgtgcgg atgcggacca
     5101 cacggtcctt ctccgcggaa agataaccct gccggatggc gacgccttcg ccctgcggcc
     5161 gccagcgcga aacatcgacg cggaacttgt gctcgatctc ctgacccatc tgcccctcga
     5221 atgttgacgc ggtagctgtc gcgccgatac ttgcggcatg gaagttccgc aaaggccgcg
     5281 tgtcggcctc ttcgtcacct gcctcgtcga tctgtttcgc ccgtcgatcg gctgggcgac
     5341 cgtcaagctg ctcgaagatg ccggctgccg ggtagaaatc ccggtgcaaa catgttgcgg
     5401 acagcccgat tataattccg gcgaccgccg cttggccaaa gatctcgc
//
LOCUS       DASWOG010000096         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_258893,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000096 DASWOG010000000
VERSION     DASWOG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258893"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1033
                     /locus_tag="VGB93_01955"
     CDS             122..1033
                     /locus_tag="VGB93_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0144925.1"
                     /translation="MARASWAGKGVAPVALVLATLCLAGCQTPGVGDITGSIGQDSVP
                     PTSGPELQRYTEDLGQRYDQQPYDKATAMAYARALRASGRYDQAVAVAQRLAAKYPKD
                     MQVLGAYGKALVEDGRLQEAASVLPEAHTPDRPNWSILSAQGTVADQLGDHDQAQDYY
                     RAALKIAPNQPAVLSNLGLSYALSKQLPEAEQTLQLAAAQPGADTRVRQNLALVLALE
                     GKFNAAQGVAQEDLAPVDAAENVASIRQMIAQSDTWNQIQSYDKPKVATASHRIRHYA
                     RLDKTKTQQNAQQNPQDLRPTQIGAAD"
     gene            complement(1102..2073)
                     /locus_tag="VGB93_01960"
     CDS             complement(1102..2073)
                     /locus_tag="VGB93_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0144926.1"
                     /translation="MDDLSQTLLDRQRILAALVAIATIATILTVTIPLFETDRFSRRM
                     KIIASERERIRARERDRLNESKISLRRQPKAYMKNIVDRFNLSSWLGTDKAKKQMIMA
                     GFRGPQAEVAFLFVRLVTPIILFLLTLLYLFFLSNFGWSNIMKIGVAIGALYIGLKAP
                     EIYISNTIAKRQQSMGRAVPNALDLMLICVESGMSIEPAFRKVAQEIGAQSVPLAEEL
                     TLTTAELSYLSDRRQAFENLGARTGLDSLKQISTVLTQAERYGTPLGQALRVVADESR
                     QKRMSAAEKKAAALPPKLTVPMILFFLPVLFAVIITPAIIQVQASMR"
     gene            complement(2101..>2459)
                     /locus_tag="VGB93_01965"
     CDS             complement(2101..>2459)
                     /locus_tag="VGB93_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0144927.1"
                     /translation="ERLATQVPISETSFFAIVINIQQKAGGNLSEALSNLSGVLRDRK
                     MMEGKIKAFSSEAKASAMIIASLPFIVAGLVYITSPAYISLLWEKPSGQLVLAGSAIW
                     MSIGVFIMNKMISFDY"
BASE COUNT          542 a          684 c          730 g          503 t
ORIGIN      
        1 aggcttctta acgctttatt agggttaact ttttatttct aacaggcggt aggaatagaa
       61 aggtctggcg atgcgcaggt ctagcgccaa caagtctcgc gctacaaggc aatctgccag
      121 tttggcgagg gcgtcgtggg ccggtaaagg cgttgcaccg gtcgctctgg tcctggccac
      181 gctttgtctc gcgggctgcc aaacccctgg cgtcggcgat atcaccggct cgatcgggca
      241 agacagcgtg ccgccaacct caggcccgga attgcagcgt tataccgagg acctcggtca
      301 gcgatacgat cagcaaccct acgacaaagc gaccgcaatg gcctatgcac gtgcactgcg
      361 ggcaagcggc cgttacgacc aggcggtcgc cgtcgcacaa aggcttgcag caaagtatcc
      421 aaaagacatg caggtgctgg gcgcctatgg caaggcgtta gttgaggatg ggcgtttgca
      481 ggaggccgcg agcgtcctgc ctgaggcgca caccccagat cggcccaatt ggtctattct
      541 ttcggcacaa ggcaccgtcg cggaccagct cggcgatcac gatcaggcgc aggattatta
      601 tcgcgcggcg ctcaagattg cgccaaacca gccggcagtc ttgtccaacc ttggtctatc
      661 ttacgcttta tccaagcaat tgcctgaagc ggagcagacg ttgcagctcg ccgcggcgca
      721 gcccggggcc gacacgcgcg tccggcaaaa tctcgcgctg gtcctggcgc tcgaaggcaa
      781 attcaacgcg gcgcagggtg ttgctcagga agatctggcg ccggtcgatg ccgccgagaa
      841 tgtcgcgtcg atccggcaaa tgattgccca gtcggatacc tggaatcaga tccaaagcta
      901 tgacaagcca aaagtggcca ctgcgtcgca tcggatccgc cattacgccc ggctcgataa
      961 aacaaagacg caacaaaatg cgcagcagaa tccgcaggat ctgcgcccga cacagatcgg
     1021 cgcggccgat taggccgcga taattgtctc gataattcgt atcttggtcg ccgcgcatcg
     1081 ttcctgatgc cacgggacac ctcagcgcat cgacgcctgg acctggatta tagctggggt
     1141 gatgattacc gcgaacagta ccggcaaaaa gaacaggatc atcggcacag taagttttgg
     1201 cggcaacgcg gcggctttct tttccgctgc gctcatgcgt ttttggcggc tttcatcggc
     1261 gacgacgcgg agggcctgac cgagaggcgt gccgtagcgt tccgcctgcg tcaacacggt
     1321 cgaaatttgc ttcaatgagt cgaggccagt ccgggcgccg agattttcaa aagcctgacg
     1381 ccgatccgat agataagaaa gttcagccgt cgtcagagtc agttcctcgg cgagcggaac
     1441 ggattgagcg ccgatttcct gcgcgacctt gcggaacgcc ggctcgatgg acatgccgga
     1501 ttcaacgcag atgagcatga gatcgagcgc gttcggtacg gccctgccca tcgactgttg
     1561 tcgtttggcg atggtattgc tgatatagat ttccggcgct ttcaggccaa tgtacagggc
     1621 gccgattgca acgccgattt tcataatatt ggaccagccg aaattgctta ggaagaagag
     1681 atagagaagc gtcagcaaga acaggatgat gggtgtgaca aggcggacga agaggaaggc
     1741 aacctcagcc tgcggccccc ggaagccggc catgatcatc tgcttttttg ccttatccgt
     1801 ccccagccat gaggacagat tgaagcgatc gacgatgttt ttcatatagg ccttgggctg
     1861 ccggcgcagg ctgattttcg attcgttcag ccggtcgcgt tcacgggcac ggatacgctc
     1921 gcgttcggag gcgatgatct tcatgcgtcg gctgaaccgg tccgtctcaa agagcggaat
     1981 ggtgaccgtt agaatagtgg cgattgtcgc gattgcgaca agggccgcca agatcctttg
     2041 ccggtcgagc agtgtttgac tcagatcgtc catggccgtc cgcctgtgag aaacccagcg
     2101 tcagtagtcg aagctgatca tcttgttcat gatgaatacg ccgatcgaca tccagatcgc
     2161 gctccccgcg agaacgagct gaccgctcgg cttttcccaa agaagcgaaa tgtaggcagg
     2221 gctcgtgata tagaccaaac cggcgacgat gaacgggagg gacgcaatga tcatggctga
     2281 ggcttttgcc tcggacgaga aagccttgat tttgccttcc atcatcttgc gatcgcgcag
     2341 cacgccggaa aggttcgata aagcttcgga aagatttccg cctgccttct gctgaatgtt
     2401 gatgacgatc gcgaaaaagc tcgtctcaga aatcggtact tgcgtcgcca gccgctcgc
//
LOCUS       DASWOG010000097         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_112286,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000097 DASWOG010000000
VERSION     DASWOG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112286"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGB93_01970"
     CDS             complement(<1..229)
                     /locus_tag="VGB93_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY0144928.1"
                     /translation="MTETSSSRLLAGSPYPLGATWDGLGTNFAVFSAHAEGVDLCLFD
                     PSGRREISRLPLPECTDEVWHGYLPNARAGFI"
     gene            complement(226..2298)
                     /gene="glgB"
                     /locus_tag="VGB93_01975"
     CDS             complement(226..2298)
                     /gene="glgB"
                     /locus_tag="VGB93_01975"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0144929.1"
                     /translation="MLPGAEAVEIIAQTDGRHLASMMRGAVEGLFEARVDSSAPYKFR
                     ISWPGAEQETEDAYTFGLLLGDIDIYLFNEGRHFDFADVFGAQPMVIDGIHGVRFSVW
                     APNAQRVAVIGDFNAWDTRRHLMRARYPSGIWELFIPRVSVGARYKFAIVGPHGEHLE
                     KADPVARWAELPPATASVVAERGEFEFHDEHWMAGRAARHAVDVPLSIYEVHLASWLK
                     ADGPASSGWNLAIERLIPYAVEMGFTHLELLPIAEHPFGGSWGYQPLGLFAPTSRYGT
                     PRDFARFIDAAHTAGIGVIVDWVPAHFPNDAHGLARFDGTALYEHLDPREGVHRDWNT
                     FIYNFGRREVQGFLVANALFWLERFHVDGLRVDAVASMLYRDYSRAAGEWVPNIYGGR
                     ENLEAIGFLKHLNSVVRERCPGAMMIAEESTAFPGVTRSVEEGGLGFTYKWNMGWMHD
                     TLLYMERDPIHRPYHHDEITFGLLYAFSENFILPLSHDEVVHGKKSLISKMPGDRWQR
                     FANLRSYFGFMWAHPGKKLVFMGADVAQEHEWSHDSEVEWSRLNESPNAGVQKLLRDL
                     NQLYKSEPALHQRDVEPSGFQWLIGDDRANSVFAFLRHGRQGTPPVLAVCNMSPVPRP
                     NYRVGVPHSGAWREIFNSDAALYGGSNVGNGGEVIASAQPMHGEAQSLEILLPPLAVV
                     FFRGGCDR"
     gene            complement(2420..5716)
                     /gene="treS"
                     /locus_tag="VGB93_01980"
     CDS             complement(2420..5716)
                     /gene="treS"
                     /locus_tag="VGB93_01980"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY0144930.1"
                     /translation="MNMNVQSAQITGTQPNDPLWYKDAVIYQVHVKSFFDANNDGIGD
                     FAGLIEKLDYIAGLGATAIWLLPFFPSPRLDDGYDISDYRSVHPDYGGMGDVRRFIQA
                     AHVRGIRVIIELVINHTSDQHPWFQRARNAKPGSAARDFYVWSDTDQKYLGTRIIFVD
                     SERSNWTWDPVAGAFYWHRFYSHQPDLNYDNPRVLREILAIMRFWLELGVDAFRLDAI
                     PYLIERDGTSNENLPETHAILRRIRAELDTKFKGKMLLAEANQWPEHAKDYFGEGDEC
                     HMAFHFPLMPRMYMAIGREDRFPITDIMRQTPPIPENCQWAIFLRNHDELTLEMVTDM
                     ERDYLWQTYAADRRARINLGIRRRLTPLMERDRRRIELMNCLLLSMPGTPVIYYGDEI
                     GMGDNIHLGDRNGVRTPMQWSPDRNAGFSRADPEKLVLPTIMDPLYGYEAINVEAQTR
                     DPHSLLNWTRRMLAVRGKHQAFGRGTLRFLFPKNRKILAYLREDDGETLLCVANLAHS
                     PQAVEIDLSEYAGWVPVELNNGSLFPPIGQLTYLLTLPPFGFYWFLLAKEGEWPAWHT
                     PAPEQMPEYQTFVLNDGLVSALTKSHRTLFEREVLPPYLAKRRWFSAKDQAIQQVRIV
                     DLAHIPGAKREIFFCDIEVRTASGTSRWLLPLAIVWEDEPLLALPGQLALARVRENRR
                     VGFLTDAFSVAEFARNALAAIVQGARLPLPTARIQFEGMPGSADALRLAPDADIEWMT
                     VEQSNSSLIVGDAVMLKMFRRISCGQHPEVEMGRYLTELGYTNSPQLLGEVAHVTTDG
                     IRSSLAIAQRYIRNQGDAWSWLTDDMSRTLDNLSASESAGFIDHIADHEKLATTIGRR
                     LAEMHGVLARATENPEFAPAKAADYEVVLWRERVHKELVAALDIVLRHKWEKPEHQAD
                     AQILLEHGDNLLSSLDTLAQGAAGALICRIHGDFHLGQVLVAAGDVYIIDFEGQPSTP
                     IEDRRHKESPLRDVAGLLRSLDYAAATAAQGRGVSASTGNQEARRAFITRFRDATTSA
                     FLSAYRESAERYGEVLNESLLDLFMIEKSAYEIRYEAANRPTWLPIPLAGLAGLTRRL
                     LSRGVLHGER"
     gene            complement(5713..>7683)
                     /locus_tag="VGB93_01985"
     CDS             complement(5713..>7683)
                     /locus_tag="VGB93_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY0144931.1"
                     /translation="EVLEHAIRSPRIVIEAVQPSVDDGAFPARCVTGDAVQVSADIFS
                     DGHPVLAAEVLWQGIDDSNWQRAPLHLGYNDRWSGTFSPNRIGPHRFTVEAWIDEFAG
                     LLHDLEVKHKAGVVAKADVLEAKAFVQSLSGVSAKSRLKLENLAQSLDGASAEQAVAI
                     LTDDTNQAAIRSAQPRRFSLRAQAIPLDVERQKAVFSSWYELFPRSTSRVAGQHGTFD
                     DVIARLPQIRAMGFDVLYLTPIHPIGRTNRKGRNNSLVSTPEDPGSPYAIGAAEGGHD
                     AIHPQLGSFEDFASLVASARDHGLELALDFAVQCAPDHPWLKEHRDWFRWRADGSMRF
                     AENPPKKYEDIINVEFYAEPDAAALWTALRDVVLFWVEHGVKIFRVDNPHTKPLPFWK
                     WMISDIRARYPDVIFLSEAFTRPKMMYRLAKVGFSQSYTYFTWRNTKREITDYITELT
                     APPVADFFRPHFFVNTPDINPLFLQNSGRAGFLIRAVLAATLSGLWGVYSGFELCEAA
                     ALPGREEYLDSEKYELKHRDYDVPGNIKAEITMLNAIRVANPALQSHRGVKFYNAFND
                     EIIVYGRKSPASDEMIFVIVNLDPHNVQEATFEMPLWEWNLGDEQSLRIEDLLHPGQQ
                     MVWTGKLQHIRLDPVVLPYAIWRFAPLDRGRT"
BASE COUNT         1718 a         2244 c         2229 g         1492 t
ORIGIN      
        1 aaatgaagcc tgcccgcgca ttcggcagat aaccgtgcca gacttcgtcc gtacactcgg
       61 gtaacgggag acgggaaatt tcccgccggc cagacggatc gaaaaggcat aggtcaaccc
      121 cttccgcgtg cgcggaaaac accgcaaaat tcgtacccag gccgtcccag gtcgcgccaa
      181 gtggataggg cgaacccgcc agtaaacgcg acgaagatgt ttctgtcatc gatcacaccc
      241 gccccggaaa aaaacgacgg cgagtggcgg cagtaagatt tccaaagact gcgcctcgcc
      301 atgcatcggc tgcgcggacg cgatcacctc gccgccgttt ccgacatttg agccgccata
      361 gagcgcagca tcgctattga agatttctcg ccaggccccc gaatggggaa cgccgacccg
      421 atagttcgga cgcggcaccg gcgacatatt gcagaccgcg agaacgggcg gcgtgccctg
      481 ccgtccatgg cgcagaaatg cgaagaccga attggcgcga tcgtcaccga tcagccattg
      541 aaagccgctt ggctcaacat cgcgctgatg caaggcgggc tcggacttgt aaagctgatt
      601 gagatcacgg aggagcttct gcacgccagc gttagggctc tcgtttaatc tggaccactc
      661 gacttcgcta tcgtgcgacc attcatgctc ctgagcgaca tccgcgccca tgaacacgag
      721 tttcttgccg ggatgcgccc acataaaacc gaaataggag cgaaggttcg caaaccgttg
      781 ccagcgatcg ccaggcattt tgctgatcag cgattttttt ccgtgcacga cttcatcatg
      841 cgacaacggc aagataaaat tttccgagaa agcgtagagg agtccgaagg tgatctcgtc
      901 gtgatgatac ggccgatgga tgggatcgcg ctccatatag aggagtgtgt cgtgcatcca
      961 gcccatattc catttgtagg tgaagccgag ccccccctcc tccacagacc gcgtgacgcc
     1021 cgggaatgcc gtcgattcct cggcgatcat catcgccccg gggcaacgtt cgcgaacgac
     1081 gctgttgaga tgttttaaaa agccgatcgc ttcgagattt tcccgaccgc cgtaaatgtt
     1141 cggcacccat tccccggctg cgcggctgta atcgcggtaa agcatcgaag cgacggcatc
     1201 cacgcgcaac ccgtcaacat gaaatcgttc aagccagaag agcgcattgg cgaccaggaa
     1261 gccctgtact tcgcggcggc caaaattata gataaacgta ttccaatcgc gatgaacgcc
     1321 ttcgcgcgga tcaagatgtt cgtagagtgc cgtcccgtcg aaacgggcca gcccatgggc
     1381 gtcgttcggg aaatgcgccg gcacccaatc gacgatcaca ccaattccgg ctgtatgcgc
     1441 cgcgtcgatg aagcgtgcaa aatcccgcgg cgtgccatag cggctggtgg gcgcgaaaag
     1501 acctagcggc tgatagcccc aggaacctcc aaatgggtgc tccgcgatcg gcaataattc
     1561 gagatgcgtg aatcccattt cgaccgcata cggaatgagc cgctcgatcg cgaggttcca
     1621 gccggacgaa gcgggcccgt cggccttcag ccacgaggca agatggactt cataaatgga
     1681 gagcggcaca tcaaccgcat ggcgcgctgc gcgccccgcc atccaatgtt cgtcgtgaaa
     1741 ctcgaattcg ccgcgttccg caacgacgga ggcagtcgcg ggcgggagct ctgcccatcg
     1801 ggcgaccgga tctgccttct ccaaatgttc gccatgcggg ccgacgatcg cgaatttgta
     1861 acgcgcgccg actgaaacac gtgggataaa caactcccaa atcccagaag gatagcgtgc
     1921 ccgcatcaga tgccggcgtg tatcccaggc attgaagtcg ccaatgacag ccacacgttg
     1981 cgcgttcggc gcccaaaccg aaaagcgcac accatgaatg ccgtcgatca ccatcggctg
     2041 cgcgccaaaa acgtcggcga agtcgaaatg gcgcccttcg ttgaagagat aaatatcgat
     2101 gtcgccgaga agcaatccaa acgtataagc gtcttccgtc tcttgctcag cgcccggcca
     2161 tgaaatccga aatttgtaag gcgcactgct atcgacacgg gcttcaaaca acccttcgac
     2221 cgcgccgcgc atcatggacg cgaggtgccg accgtccgtc tgtgcgataa tctcaacggc
     2281 ttccgcgccc ggcagcatgg cgcggatcac ccggccatca ccgccgtcgt gcgggccgag
     2341 aattgcgaaa ggatccacga atgcgtttcc ggcaagcttt tgcgcttcgc ggcggtccaa
     2401 gtgccaacag aacgtctgat caacgctcgc catgtaaaac cccgcgtgaa aggagccgcc
     2461 gcgtgagacc cgcaagccca gcgagcggga tcggcagcca tgtcggccgg ttcgcggctt
     2521 cgtagcgaat ctcgtaggca gacttttcaa tcatgaacag gtccaacaaa gattcgttca
     2581 gcacctcgcc gtagcgctca gcgctttcgc gataggccga gagaaatgcc gatgtcgttg
     2641 cgtcgcgaaa ccgggtgatg aaggcgcggc gcgcctcttg gttcccagtc gaagcgctta
     2701 cgcccctgcc ctgcgccgcc gtcgccgcgg cataatccag cgagcgcaaa agccccgcga
     2761 catcgcgcag cggactttct ttatgtcggc gatcctcaat cggcgtggac ggctgtcctt
     2821 caaagtcgat gatgtaaacg tcaccggccg ctacaagcac ttgccccaga tgaaaatcgc
     2881 cgtggatccg gcatatcagg gcgcctgcgg cgccctgcgc aagagtatcg aggctcgata
     2941 gcagattgtc accgtgctca agcagaattt gcgcatcggc ctgatgttcc ggcttttccc
     3001 atttgtgccg aaggacgata tcgagtgcgg ctaccagctc cttgtggacc cgctcgcgcc
     3061 acagcacgac ctcataatcc gccgctttcg ccggcgcaaa ctccggattt tcagtcgccc
     3121 tggcgaggac gccgtgcatt tcggcgagcc gccggccgat tgtcgtcgcg agcttttcgt
     3181 gatcggcgat gtggtcgatg aaaccggcgc tttcggaggc ggacaaatta tcgagcgttc
     3241 tcgacatgtc gtccgtcaac cacgaccagg catcgccctg attacgaatg taacgctgcg
     3301 cgatcgcgag ggaggaacgt atcccgtccg tcgtcacatg cgcgacctcg cccaaaagct
     3361 gcggcgaatt cgtgtagccg agctcggtca aataacggcc catttcgact tccgggtgct
     3421 ggccgcagga gatgcgccgg aacatcttga gcatcaccgc atcgcccaca atgagcgaac
     3481 tgttggattg ctcgacggtc atccactcga tgtccgcatc aggcgccaaa cgcagagcgt
     3541 cagcgcttcc gggcatgcct tcgaattgaa tgcgagcggt cggcaacggc agacgcgcgc
     3601 cttgaacgat cgctgcgagc gcattgcgag cgaattcagc gacggagaag gcatcggtca
     3661 gaaaaccgac gcgacgattt tcacgcacgc gcgccagcgc gagctgtccg gggagtgcca
     3721 gcagcggctc atcttcccaa acaatggcaa gcggcaatag ccagcgtgac gtaccgctcg
     3781 cagtccgcac ctcgatatcg caaaagaaaa tctcgcgttt cgcgccggga atatgagcaa
     3841 gatcaacgat tcgcacctgc tggattgcct ggtcctttgc cgagaaccag cggcgcttgg
     3901 cgagataagg cggcaacacc tcgcgctcga aaagcgtgcg atgcgatttg gtcagggcgc
     3961 tcaccagccc gtcgttcaag acgaaagtct gatattcggg catctgttcg ggcgccgggg
     4021 tatgccatgc cggccattcg ccttctttcg ccaacaggaa ccagtaaaac ccgaagggcg
     4081 ggagcgtcag caaataggtg agttggccga taggcgggaa gagtgatccg ttgttgagtt
     4141 cgaccggaac ccaacccgca tattccgaga gatcgatttc aaccgcttgc gggctgtggg
     4201 cgagattggc gacgcagagc agagtttcgc cgtcgtcttc acgaagatag gccaagatct
     4261 tgcgattctt cggaaagagg aaacgcagcg tgccgcggcc gaaagcttga tgcttgccgc
     4321 gcacggccag catccgccgc gtccagttca acagcgaatg cggatcgcgc gtctgcgcct
     4381 cgacgttgat cgcttcatat ccgtagagcg gatccataat cgtcggaagt acgagtttct
     4441 ccgggtcggc acgagagaaa ccggcattgc gatccggcga ccattgcatc ggcgtgcgca
     4501 cgccattgcg atcgccgaga tggatgttat cgcccatacc aatctcgtcg ccgtagtaga
     4561 tgaccggcgt tccaggcata gagagcaaaa gacaattcat cagctcaatg cggcgccggt
     4621 cacgttccat caagggtgta aggcgacggc gtatgccgag attgatacgc gcccggcgat
     4681 cagcagcata agtctgccag agataatcgc gctccatgtc ggtgaccatt tcgagggtca
     4741 gttcgtcgtg gttgcgcaag aagatcgccc attggcaatt ttcgggaatt ggcggcgtct
     4801 ggcgcataat atcagtgatc ggaaaccggt cctcgcggcc gattgccata tacatgcgcg
     4861 gcatgagcgg aaagtggaag gccatatggc attcatcgcc ttcgccgaaa taatccttcg
     4921 catgttcggg ccattgattg gcttccgcca acaacatttt gcctttgaat tttgtatcga
     4981 gttccgcccg gatgcggcgc aatatggcgt gcgtctcggg gaggttttcg ttgctcgtcc
     5041 cgtcgcgctc aatcaaatag ggaatcgcat caagtcggaa cgcgtcgact ccgagttcga
     5101 gccaaaaacg catgatcgca agaatttcgc gaagcacgcg cggattgtcg taattgaggt
     5161 ccggctgatg cgaatagaac cgatgccagt agaatgcccc cgcgaccggg tcccatgtcc
     5221 agttcgagcg ctcgctatcg acgaagatga tgcgcgtgcc aagatatttt tgatcggtat
     5281 ccgaccagac atagaagtcg cgcgccgcgg agcctggttt tgcattgcgg gcacgctgaa
     5341 accagggatg ctgatcggat gtgtgattga tgacaagttc gatgatgacg cggatgccgc
     5401 gcacatgcgc tgcctgaatg aagcgacgca catcgcccat gccaccgtaa tcgggatgaa
     5461 ccgatctata atcactgata tcataaccgt catcgagacg cggcgagggg aaaaatggca
     5521 acagccaaat ggccgtcgcc ccgagaccgg cgatatagtc gagcttctct atcagccccg
     5581 cgaaatcgcc gatcccatcg ttgttggcgt cgaagaacga tttgacgtga acttgataga
     5641 tgacagcatc tttataccaa agcggatcat ttggctgcgt gccggtaatc tgcgccgact
     5701 gtacattcat gttcatgtgc gcccccggtc gagcggcgcg aaacgccata tggcataggg
     5761 caacacaacg gggtcaaggc gtatatgctg cagctttcct gtccaaacca tctgttgacc
     5821 aggatgaagc aagtcttcga tgcggagcga ttgctcgtcg cctagattcc attcccagag
     5881 cggcatctcg aacgtggctt cctgcacatt gtgcggatca agattgacga tcacgaagat
     5941 catttcgtcg ctcgcgggcg acttccggcc gtagacaatg atttcatcgt taaaggcgtt
     6001 gtaaaatttg acgcctcggt gactttgcaa cgccgggttc gcgacgcgga ttgcgttcag
     6061 catggtaatt tcggctttga tatttccggg cacgtcgtag tcgcggtgct tcagttcgta
     6121 tttctcggaa tcgagatatt cctctcgccc gggcaacgcc gccgcctcgc aaagctcgaa
     6181 gccggaatag acgccccata aacccgagag tgtcgccgcg aggacggcgc ggatgagaaa
     6241 accggcgcgg ccagaattct gcagaaagag cggattgata tccggcgtgt tgacgaaaaa
     6301 atgcggccgg aaaaaatccg cgaccggagg cgccgtcagt tcggttatgt agtcagtgat
     6361 ctcgcgcttg gtattgcgcc aggtgaaata tgtataagac tgggaaaagc cgaccttcgc
     6421 cagccgatac atcatcttcg gacgcgtgaa ggcctcggaa agaaatatta cgtccggata
     6481 gcgggcgcgg atatcggaaa tcatccactt ccagaaagga agcggcttgg tgtgtggatt
     6541 gtcgacacgg aagattttaa cgccgtgctc gacccagaaa agcacaacat cgcggagcgc
     6601 tgtccaaagc gccgcggcgt caggctcggc atagaattcg acgttgatga tatcttcgta
     6661 tttcttcggc gggttctcgg cgaaacgcat cgatccgtcg gcgcgccagc gaaaccaatc
     6721 ccgatgctct ttcagccacg gatgatccgg cgcacattga acggcgaaat cgagggcaag
     6781 ttcgagcccg tggtcacgcg ccgatgcgac gagactcgca aaatcctcga aagagccgag
     6841 ctgcgggtgg atggcgtcgt gaccgccttc ggccgcgccg atcgcatagg ggctgcccgg
     6901 atcttctggc gtgctgacca gactgttgtt gcggccctta cggttcgtcc ggccgatagg
     6961 atgtatcggc gttaaataaa gaacgtcgaa acccatggcg cggatttgcg gaagccgcgc
     7021 gatcacatcg tcaaacgtac cgtgctgccc cgcgacacgc gacgtcgagc gcggaaacaa
     7081 ttcataccaa gaggaaaaga cggccttttg ccgttccacg tcgagcggga tcgcttgcgc
     7141 ccgcaaggaa aaccggcgcg gctgggcgga tcggatcgcg gcctgattgg tgtcgtccgt
     7201 taggatggcg acagcctgct ccgccgacgc accgtcgaga ctctgcgcta ggttctcgag
     7261 ttttagtcga gattttgcgc tgacgccgga taaagattgg acgaaggctt tggcttccag
     7321 cacatcggct ttcgcaacga cgccggcttt atgcttgacc tcgaggtcgt gcagtaggcc
     7381 ggcgaattcg tcgatccagg cctcaaccgt gaaccgatgc ggtccgattc gattcggtga
     7441 gaaggttccg ctccatcgat cattataacc aaggtgcagc ggcgcgcgtt gccaattgga
     7501 atcgtcaatg ccttgccaaa gaacttccgc ggcgagcacg ggatgcccat cggagaaaat
     7561 atcagccgag acctgcaccg catccccagt cacgcagcgc gcgggaaaag cgccgtcatc
     7621 gacagagggc tggaccgcct caatcacgat gcgcggtgaa cgaatggcgt gttccagcac
     7681 ttc
//
LOCUS       DASWOG010000098         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_350491,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000098 DASWOG010000000
VERSION     DASWOG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350491"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VGB93_01990"
     CDS             complement(<1..1107)
                     /locus_tag="VGB93_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0144932.1"
                     /translation="MAEFDTDLFVIGGGSGGVRAARIAAGHGARVMIAEEHRMGGTCV
                     IRGCVPKKLLVYASHFHQDIEDAAGFGWTIPSATFDWPTMIANKDREIARLETAYTTN
                     VEKSGARVVKTRAVFEDAHTLRLSTGETIRSKTILIATGGAPNHGQAIPGIEHVISSN
                     EVFHLPTLPRRILIQGGGYIALEFASIFAGFDCDVTLVYRGDNILRGSDEDVRDHVRS
                     EMEKAGITILTGCTVNKIDKHGKEFTSHLSNGSSIASDQVMFAIGRNPNIGGLGLEKA
                     GVAINPNNGGIQVDSFSRTSVPNIYAVGDVTHRINLTPVAIREGHAFADTVFGKRTIE
                     VDHADIPTAVFCQPEVGTVGLTETQARAQFSHVDI"
     gene            complement(1210..1731)
                     /locus_tag="VGB93_01995"
     CDS             complement(1210..1731)
                     /locus_tag="VGB93_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY0144933.1"
                     /translation="MKSLSQIMLAAGLAVGLAFSIAPAGAQTPAPAKPASPAAIAAAK
                     EILDMKKASAMYAQAVPSVVEQTKTQLMQTNLNYQRDLNEVAIIVAQSLAGREKEIGD
                     GMAKVYADAFTEQELKDLVTFYKTPLGQKLLTAEPEAIQSSIAFMRQWGQTFSQTVAA
                     QFRAEMHKRGKDI"
     gene            complement(1754..2452)
                     /gene="rpiA"
                     /locus_tag="VGB93_02000"
     CDS             complement(1754..2452)
                     /gene="rpiA"
                     /locus_tag="VGB93_02000"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY0144934.1"
                     /translation="MNMDELKRQAAARALEYVRDGMKLGLGTGSTAKHFVELLGERVR
                     AGLKVIGVPTSETTRADAVRCGIALTTLDEIDRLDLTVDGADEIDPDLNLIKGGGGAL
                     LREKIVAAESDRMIVIADDSKWVEALGRFPLPIEVIPFGLAATQRAIGHAFAECGVSG
                     PMVVRKRQDGHVFVTDGGHWIVDAHLGRISQAKQLAGLLTAIPGVVEHGLFIGLASTA
                     MLAGSQGIRVVERR"
BASE COUNT          490 a          822 c          762 g          545 t
ORIGIN      
        1 gatatcgacg tgactgaatt gtgcgcgcgc ctgggtttcg gtcagtccca ccgtgccgac
       61 ttccggctgg cagaacaccg cggtcggaat gtcggcatga tcgacctcaa tcgtccgctt
      121 gccgaacacg gtatcggcaa aggcatgccc ctcgcggatc gcgaccggcg tcaaattaat
      181 gcggtgggtg acgtcgccga ccgcgtagat attcggcact gaagtccggg aaaaactgtc
      241 gacctggatt ccaccattgt tcggattgat cgcgacgccg gccttctcaa gcccaaggcc
      301 cccgatgttc ggattcctgc cgatcgcaaa catcacctgg tcggaagcga tgctggatcc
      361 attcgacaga tgcgaggtga attccttgcc atgcttgtcg atcttgttga cggtgcagcc
      421 ggtcaggatg gtaatccccg ccttttccat ctcgctgcgc acatgatcgc gaacatcctc
      481 gtcagagccg cgcagaatat tgtcgccacg atagaccagc gtcacgtcgc agtcgaagcc
      541 ggcaaagatg ctggcgaatt ccagcgcgat ataaccgccg ccctgaatca ggatgcggcg
      601 tggcaacgtc ggcagatgaa acacctcgtt ggaggagatc acatgctcga tgccggggat
      661 cgcttgcccg tggttcggcg cgccgccggt cgcgatcaga atggttttcg aacggatcgt
      721 ctcgccggtc gaaagccgca gcgtatgggc gtcctcgaac accgcgcggg tcttgacgac
      781 gcgcgcgccg gatttctcga cattggtggt gtaggccgtc tctagccggg cgatctccct
      841 gtccttgttg gcgatcatgg tcggccagtc gaaggtcgcc gacgggatgg tccagccgaa
      901 tcccgcggcg tcctcgatgt cctgatggaa gtgggacgca tagaccagaa gcttcttcgg
      961 cacgcagccg cggatcacgc aggtgccgcc catccgatgt tcttcagcga tcatcacccg
     1021 cgcgccgtgg ccggccgcga tccgagcggc gcgaaccccg cccgagccac cgccaatgac
     1081 aaacagatcg gtgtcgaact cagccatacc agcctcgaat gtcagcctcg atcagataga
     1141 agatcggata tccccgatag atatcctttc agatacccat tgccagatgt ccttttcagc
     1201 tatccttgtt cagatgtcct tgccgcgctt gtgcatttcg gcgcggaact gggcggcgac
     1261 ggtctgcgaa aacgtctgcc cccattgcct catgaaagcg atgctggact gaatggcctc
     1321 cggctccgcc gtgagcaact tctggccgag cggcgttttg tagaatgtga cgaggtcctt
     1381 caattcctgc tcggtgaagg cgtcggcgta aaccttcgcc atgccgtccc cgatttcctt
     1441 ctcgcggccg gccaggctct gcgcgacaat gatcgcgacc tcgttgaggt ccctttgata
     1501 attgagattg gtctgcatca actgggtctt ggtctgttcg accacgctgg gcacggcctg
     1561 cgcgtacatc gcgctggctt tcttcatgtc cagaatttcc tttgcggctg cgatcgccgc
     1621 gggcgaagcc ggcttggccg gcgccggcgt ttgcgcgccc gccggggcga tcgaaaacgc
     1681 cagtcccact gccagacccg cggccaacat gatttgcgaa agactcttca ttttattctc
     1741 ctcgatggcg gcgttaccgc cgttctacaa cccgaattcc ctgagagccc gccagcatcg
     1801 cagtgctggc caggccgatg aatagtccgt gttcgacgac gcctggaatg gcggtcagca
     1861 atcccgcaag ttgctttgcc tgagatatgc gccctaaatg ggcatctaca atccagtggc
     1921 caccatcggt gacgaaaacg tggccgtcct gacgctttcg gaccaccatc gggccggaaa
     1981 cgccacattc ggcaaaagcg tgcccgatcg cgcgttgtgt cgccgcgagg ccaaacggga
     2041 tgacctcgat cggcagcggg aaacgaccaa gcgcctcgac ccatttcgaa tcgtccgcaa
     2101 tcacgatcat gcggtccgac tccgctgcta cgatcttttc ccgcagcaac gcgccgccgc
     2161 cgcccttgat cagattgagg tcggggtcga tttcgtcggc gccatcgacg gtcaaatcga
     2221 gccggtcgat ctcgtcgagc gtggtcaggg caatgccgca gcgcaccgcg tcggcgcggg
     2281 tcgtctctga agtgggcacg ccgatcacct tgagaccggc gcggacccgc tcgcccaaca
     2341 gttcgacaaa atgttttgcg gtcgaaccgg tgccgagccc gagcttcatg ccgtcccgca
     2401 catattcgag cgcacgagcc gccgcctgcc gtttcaattc gtccatattc acgggatcaa
     2461 cgatgcctca gaggccagag ccgatcgggc gatcgacggt gtccggcggg ggcatatcta
     2521 gcgtcgtttg accgggagta acagcgcttt agctgctaca attgattact tttacgtctt
     2581 gtccagttct gccttttcca aggctgaaat gatggccgc
//
LOCUS       DASWOG010000099         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_203908,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000099 DASWOG010000000
VERSION     DASWOG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203908"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VGB93_02005"
     CDS             complement(<1..1134)
                     /locus_tag="VGB93_02005"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0144935.1"
                     /translation="MSAEFLSSMRAPRGMAVAPCALAAQSALSVLRDGGNAVEAMVAA
                     AATIAVAYPHMNGIGGDGFWLIAAPGAAPIGIEACGGAAAAASIEFYRAQNLSAIPFR
                     GALAANTIAGTVSGWELALDWSRQTLGGHLPLKRLLADAIAYASDGFPVTRSQAQNTA
                     SKADELADVPGFAGTYFGADAQPPKVGALFKQKRLAQTLERLAHAGLDDFYRGDLARS
                     IAADLQALGSPLSLDDLNGYHARQREPLELKHSLGAVYNMPPPTQGLVSEIIVGLLDR
                     LLHDDMDPLGPDYVHACVESTKLAFGVRDRHITDPDYMDVDPQTFLASDKLDAMVAKV
                     SMSKALPWPRGADPADTVWLGVIDGNGVAVSFIQSVYHEFGSGI"
     gene            1359..2306
                     /locus_tag="VGB93_02010"
     CDS             1359..2306
                     /locus_tag="VGB93_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0144936.1"
                     /translation="MRDEDQPHDPSRRHALECMVWAGTGVLWTVSGGVPKSLGLLGTA
                     EAAEASQSPFTFMQISDSHIGFNKAANPHAIDTLKEAIGKVKALPVKPAFMIHTGDIT
                     HLSLASQFDDAAQIIGETGLETHYVPGEHDLIDDNNGKLYADRYGAGKHGAGWYSFDD
                     HGVHFIGLVNVVNLKAGGLGNLGPDQLAWVADDLKDKSNSTPIVVFAHIPLWTVYADW
                     GWGTDNGAQVLQLLRRFGSVTVLNGHIHQIIQKVEGHITFHTARSTAFLQPAPGKAPA
                     PGPMKVPDDQLRKVLGITRVSVSLGTEPLAITDKTLADN"
     gene            2327..2650
                     /locus_tag="VGB93_02015"
     CDS             2327..2650
                     /locus_tag="VGB93_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEY0144937.1"
                     /translation="MRIKQVLFIIALAVAGYGDASASSNVVTVAISNFTFQPTDLKVK
                     VGTTVNFKNEDDIPHRVAATDGSFYSKALDTDDVAHVTFNKPGVFPYFCTLHPHMQGK
                     IIVTR"
     gene            2679..>3027
                     /locus_tag="VGB93_02020"
     CDS             2679..>3027
                     /locus_tag="VGB93_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0144938.1"
                     /translation="MNLNASEQSAELFRQIVVPHLPDALALARWLTGSVHDAEDVVQE
                     ASIRAFKAIESYEGRGPRPWLLSIVRNTCFTWLAKNRPKEIVLAGTSVEMDETEIGLQ
                     EDPRLDPEADLIRK"
BASE COUNT          645 a          867 c          919 g          596 t
ORIGIN      
        1 gatgccgcta ccgaattcat gatagacact ttggatgaag ctcacggcga cgccattgcc
       61 gtcgatcacg ccgagccaga cagtgtcggc gggatcggcg ccgcgcggcc aaggcaatgc
      121 cttcgacatt gagactttcg ccaccatggc atcgagctta tccgacgcca ggaacgtctg
      181 cggatcgaca tccatataat cggggtcggt gatgtggcgg tcgcggacgc cgaaggcaag
      241 cttcgtcgat tcgacacagg catgcacata gtccggtccg agcggatcca tgtcgtcgtg
      301 cagaagccgg tcgagcaggc caacgatgat ttcggacacg aggccctgcg tcggcggtgg
      361 catattgtag accgcgccga gcgaatgctt cagttcgaga ggctcgcgct gtcgtgcgtg
      421 atagccgttc aaatcgtcga ggctcagcgg acttccgagc gcttgcagat cggcagcgat
      481 ggagcgggca agatcgccgc gatagaaatc gtcaaggccc gcatgcgcca ggcgttcgag
      541 cgtttgcgcc aagcgctttt gcttgaacaa ggcgccgact ttcggcggtt gagcgtccgc
      601 gccgaaataa gttccggcga agccgggcac atcggcgagt tcgtcggctt tgctcgctgt
      661 gttttgcgcc tggctgcgcg taaccggaaa gccgtccgac gcataggcga tggcatcggc
      721 cagaagacgt ttgaggggca ggtgaccgcc gagcgtttgc cgggaccaat cgagggcgag
      781 ttcccagccg ctgacggttc cggcgatcgt gttcgccgcg agcgcgccac gaaacgggat
      841 cgcgctgagg ttctgcgcac gataaaactc gatcgaagcc gcggccgccg ccccgccgca
      901 ggcttcgatg ccgatgggtg cggcgccggg cgccgcgatg agccagaagc cgtcaccgcc
      961 gatgccgttc atatgcggat aggcgaccgc gatcgtggcg gccgccgcga ccatcgcttc
     1021 gaccgcattg ccgccatcgc gcaaaaccga aagcgcgctt tgcgcggcaa gcgcacacgg
     1081 cgccacagcc attccacgcg gcgcacgcat cgaactcaaa aactcagcag acacgatctt
     1141 ctttcctcat ggataattcg gctgcgtctc tcgcacaaac ggcgcgagta agcgagagat
     1201 gtgccagcgg tgagacgcac ggcgcgtggc gcgcgaattc actatttggt gatgtcggcc
     1261 gatcgatttc gcgcagcaag cgcggaaaaa ttcaaatgcg cgggaataaa acgccgaagg
     1321 ccggagtctc atcatcggac agcaaggaga ttgccacgat gcgagacgaa gatcagcctc
     1381 acgacccctc ccgccgccat gcccttgaat gcatggtttg ggccggcacc ggcgttctct
     1441 ggacggtttc aggcggggtg ccgaaatcgc ttgggctact cggcacggcc gaagcggcgg
     1501 aagcgtcgca aagtcccttc actttcatgc agatatccga cagccacatc gggttcaaca
     1561 aggcggccaa tccgcatgcg atcgatactt tgaaagaggc aatcggcaag gtgaaggcgt
     1621 tgccggtcaa gccagcgttt atgatccaca ccggcgacat cacccattta tcgctggcgt
     1681 ctcaattcga tgacgccgcg cagatcatcg gcgaaaccgg tcttgagacg cattacgtgc
     1741 ccggtgaaca tgatctcatc gacgacaaca atggcaagct ctatgcggat cgctacggcg
     1801 cgggcaagca cggtgcgggc tggtattcct tcgatgatca cggcgtccac ttcattggct
     1861 tggtcaacgt cgtcaatctg aaagctggcg gtctcggcaa cctcggaccg gatcagcttg
     1921 cgtgggtcgc cgatgacttg aaagacaagt caaattcgac accaatcgtc gttttcgcgc
     1981 atatcccgct ctggacagtc tatgcggatt ggggatgggg aacggacaac ggcgcgcagg
     2041 ttctgcaatt gcttcgccgc ttcggttcgg tgaccgtcct gaacggccat attcaccaga
     2101 tcatccaaaa agtcgaaggc cacataacct tccatacggc gcgctcgacg gcgtttctgc
     2161 agccggcgcc tggcaaggcc cccgcgcccg gaccaatgaa ggtgccagac gaccaattgc
     2221 gcaaagtgct cggcataacg cgcgtttcag tgagtctcgg aacagagcca ctcgcaatca
     2281 ccgataagac gttggctgac aattgagtgt gcgagaggat tgcgtcatga gaatcaaaca
     2341 ggttttattt atcattgcgt tggctgttgc cggctatggt gacgcttcgg cgtcgtcgaa
     2401 tgtcgtaacc gtggcgatat cgaacttcac gttccagccg actgatctta aggtgaaagt
     2461 tggaaccacg gtcaatttca agaacgaaga cgatattccg catagggtcg cggcgacaga
     2521 cggaagcttt tattccaagg cacttgacac cgatgatgta gcgcacgtca cctttaacaa
     2581 gcccggcgta tttccttatt tctgtacgtt acatccacac atgcaaggca agattatcgt
     2641 tacgcgttga gcgtgtctgc gccaggtttt tttagggcat gaacttgaat gcaagcgaac
     2701 aatctgccga actgtttcgg caaatcgttg tgccgcatct tcccgatgcg ttggcgcttg
     2761 cgcgttggtt gaccggcagc gtgcatgacg ccgaggatgt cgtgcaagag gcctctatcc
     2821 gcgctttcaa ggcgatcgag tcctatgaag ggcgcggtcc gcgcccgtgg ctcctgagca
     2881 tcgtgcgcaa cacctgcttt acgtggctcg cgaagaacag gccgaaggaa atcgttctgg
     2941 ctggcacgtc ggtggagatg gacgagacgg agattggttt gcaggaggat ccccggctcg
     3001 atccagaggc agatctcatc agaaagg
//
LOCUS       DASWOG010000100         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_2496, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000100 DASWOG010000000
VERSION     DASWOG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="fusA"
                     /locus_tag="VGB93_02025"
     CDS             <1..696
                     /gene="fusA"
                     /locus_tag="VGB93_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447767.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0144939.1"
                     /translation="KVDILKRTYKVDANIGAPQVAYRERLTKRVEKDYTHKKQTGGTG
                     QFAKVTIIFEPNEPGAGNVFESKIVGGAVPKEYIPGVEKGINSVMGSGVLAGFPVVDV
                     KATLIDGGFHDVDSSVLAFEIAARAAFREALREGGSVLLEPIMKVEVTTPEDYTGSVM
                     GDLLGRRGQVQGQDMRGNAVVINAMVPLANMFGYVNQLRSFSQGRANYTMQFDHYEQV
                     PAAEAAKVQAKFA"
     gene            727..1917
                     /gene="tuf"
                     /locus_tag="VGB93_02030"
     CDS             727..1917
                     /gene="tuf"
                     /locus_tag="VGB93_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0144940.1"
                     /translation="MAKEKFSRNKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATF
                     TAYDQIDKAPEEKARGITISTAHVEYETKARHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDPELLELVELEVRELLSK
                     YEFPGDDIPITKGSALCALEGRNPEIGHDAVLALMTTVDDYIPQPERPIDQPFLMPVE
                     DVFSISGRGTVVTGRVERGIVKVGEEVEIVGLKPTVKTVVTGVEMFRKLLDQGQAGDN
                     IGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKDEGGRHTPFFTNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNVTMSVELIVPIAMEEKLRFAIREGGRTVGAGVVA
                     SIQE"
BASE COUNT          408 a          566 c          630 g          367 t
ORIGIN      
        1 aaggtcgata ttctgaaacg cacctataag gttgacgcca atatcggcgc gccgcaggtc
       61 gcctatcgcg agcgcctgac caagcgcgtc gagaaagatt atacgcataa gaagcagacc
      121 ggcggcacgg gccagttcgc gaaggtgacg atcatcttcg agccgaacga gccgggcgca
      181 ggcaatgtgt tcgagtcgaa gatcgtcggc ggcgccgtgc cgaaagaata tattcccggc
      241 gtcgaaaagg gcatcaacag cgtcatgggc tccggcgttc tcgctggctt cccggttgtc
      301 gatgtcaagg cgacgttgat cgacggcggc ttccatgacg tcgattcatc tgttctcgcc
      361 ttcgaaatcg ctgcgcgcgc tgcgttccgc gaggcgctgc gcgagggcgg ctcagtgttg
      421 ctcgagccga tcatgaaggt cgaagtgacg acgccggaag attacaccgg ctcggtcatg
      481 ggcgatctgc ttggccggcg cgggcaagtg caaggccagg acatgcgcgg caatgcggtg
      541 gtcatcaatg cgatggtgcc gctcgccaac atgtttggtt atgtcaatca gttgcggtcc
      601 ttcagtcagg gccgtgctaa ctacacgatg cagttcgatc actacgagca agtgcctgcg
      661 gcggaagccg ccaaggtgca ggcgaagttc gcctgaggac tgggaaaggg ttgaaggagc
      721 cgagtcatgg ccaaggaaaa attttcgcgc aacaagccgc attgtaacat cggaacgatc
      781 ggtcacgttg accatggtaa gacgtcgcta accgcggcga tcaccaaggt tttggctgaa
      841 acgggcggtg cgaccttcac ggcctatgac cagatcgaca aggcgccgga agaaaaggcg
      901 cgcggcatca cgatctcgac cgcacacgtc gagtacgaga ccaaggcgcg ccattatgcg
      961 cacgtcgatt gccccggcca cgctgactat gtgaagaaca tgatcaccgg cgccgcgcag
     1021 atggacggcg cgattctcgt cgtgtcggct gctgacggcc cgatgccgca gacccgcgag
     1081 cacattctgc ttgcgcgcca ggtcggcgtg ccggcgctcg tcgtcttcct gaacaaggtc
     1141 gacatggttg acgatccgga attgctcgaa ctcgtcgaac ttgaagtccg cgagcttctg
     1201 tcgaagtacg agttccccgg cgacgatatt ccgatcacca agggctcggc tctctgcgcg
     1261 cttgagggcc gcaaccccga gatcggccat gacgccgttc tggctctgat gacgactgtc
     1321 gacgattaca ttccgcagcc agagcgtccg atcgatcagc cgttcctgat gccggtcgaa
     1381 gacgtgttct cgatctcggg ccgcggcacg gtcgtcacgg gccgtgtcga gcgcggcatc
     1441 gttaaggtcg gtgaggaagt cgagatcgtc ggtttgaagc cgaccgtcaa gacggttgtt
     1501 accggcgtcg aaatgttccg caagctgctc gatcaggggc aggcgggtga caacatcggc
     1561 gcgctgctgc gcggcaccaa gcgtgaagac gtcgagcgcg gccaggtgct gtgcaagccc
     1621 ggctccgtga agccgcacac caagttcaag gctgaggcct acatcctcac caaggacgag
     1681 ggcggtcgtc atacgccgtt cttcaccaac tatcgtccgc agttctattt ccgcacgacc
     1741 gacgtgaccg gtgttgtcac gctgccggaa ggcacggaaa tggtcatgcc gggcgataac
     1801 gtgacgatga gcgttgagct gattgtgccg atcgcgatgg aagaaaaact gcgcttcgcg
     1861 attcgcgaag gtggccgcac cgtcggtgcc ggcgtcgtcg cttcgatcca ggaataaggt
     1921 aactcggccg gacgcgggct taagtccgcg tccgatcgtc agggaaaaaa a
//
LOCUS       DASWOG010000101         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_314177,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000101 DASWOG010000000
VERSION     DASWOG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314177"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VGB93_02035"
     CDS             <1..356
                     /locus_tag="VGB93_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144941.1"
                     /translation="DNAGNETMYSLATLTKAFVKSRRNQLVGYNVLRERVKAFSRHAA
                     VSNPRVATIATQVERLLPTRYLEHRPDKVAEANRIVEDKNLPPYVTEDPFFRTILGYV
                     LCCKASIQLDVTPAI"
     gene            400..1550
                     /locus_tag="VGB93_02040"
     CDS             join(400..649,649..1550)
                     /locus_tag="VGB93_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611490.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0144942.1"
                     /translation="MKRKRFSEEQIIANRREQEGGVATADLCRKHGMSSASLYAWKAK
                     YGGMDVSDAQKLKGLEAENGKLKRLLAEAMIDNAGLKDLPGKKMVTPAQRREAVAHLI
                     AARQMSERRACRVADVDRASVRYRSRRPDDGELRARLRSLAHERRRFGYRRLHVLLRR
                     EGWTVNRKRVQRLYREERLMVRKRGSRKRALGLRVPMPVPDRPNACWSLDFVHDQMTD
                     GRRFRVLVIVDDCTRECLGLVPDTSISGARVARELDRIIAWRGKPEAVLSDNGTELTS
                     NAILDWADERRIAWRYIQPGKPIQNAFAESFNARLRDELLNETLFRSLGHARQMIEAW
                     RHDYNHHRPHSKLGWLTPTGYATRWMQNEELEGRPSGAFNDDRIPDPAG"
     gene            complement(1668..>2032)
                     /locus_tag="VGB93_02045"
     CDS             complement(1668..>2032)
                     /locus_tag="VGB93_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144943.1"
                     /translation="IIAIMRSVEERITSSAPRDFPNRLGTDGRALDLDDELKKQILEL
                     EEQMSAQTEGFSAACIASQQAMEAENEMLFQAHELHLRWLDLRAAPRRALLVGRLKYL
                     KSLQEAFGISPQRDDNSE"
BASE COUNT          442 a          577 c          621 g          392 t
ORIGIN      
        1 tcgataatgc gggcaacgag actatgtact cattggcgac tctcacaaag gccttcgtga
       61 aaagcaggcg caatcagttg gtcggctaca atgttcttcg ggaaagagtc aaagctttta
      121 gtcgacatgc tgcagtcagt aatccgcggg tagcaacgat tgccacgcaa gtcgaacgct
      181 tgctaccaac gcgatatctg gaacatcgtc ccgacaaagt cgccgaggca aatcgaattg
      241 ttgaagacaa gaatcttcca ccttatgtga ccgaagatcc cttttttagg acaattctcg
      301 gttatgtcct gtgttgcaag gccagtatac aacttgatgt gacccccgcg atttagtcca
      361 gaaggcgcta gaatccggcc catggagagg agccggaaga tgaaacggaa gcggttttcg
      421 gaagagcaga tcatcgccaa ccggcgcgag caggaagggg gcgttgcgac ggcggatctc
      481 tgccgcaagc acggcatgtc gagcgccagc ctctacgcct ggaaagccaa gtacggcggc
      541 atggacgtct ccgacgctca gaagctcaag ggcctcgagg cggagaacgg caagctgaag
      601 cggctgttgg ccgaggcgat gatcgacaac gccggcctga aggatctcct ggcaaaaaaa
      661 tggtgacgcc cgcgcagagg cgggaagccg ttgcgcatct gattgcggcg cgtcagatga
      721 gcgagcggcg ggcgtgccgg gtcgccgatg tcgatcgcgc ctcggtgcgc taccgatcgc
      781 ggcggccgga cgatggggag ctgcgcgcgc gtttgcgatc gttggcgcat gagcgccggc
      841 gctttggcta tcggcgtctg cacgtacttc tgcgccgcga gggctggacg gtgaaccgca
      901 agcgggtgca gcggctctac cgggaggaac ggctgatggt gaggaagcgc gggagccgca
      961 agcgtgcttt gggcctcagg gttccgatgc cggtgccgga tcggccgaac gcctgctggt
     1021 cgctcgactt cgtccacgac cagatgacgg atgggcgccg cttccgcgtt ctcgtcatcg
     1081 tcgacgactg cacccgcgaa tgccttggcc tggtgcccga cacgtcgatc tcgggagcgc
     1141 gggtggcgcg ggaactcgac cgcatcatcg cctggcgcgg caagccggag gcggtgcttt
     1201 ccgacaacgg cacggaactg acgtccaacg ccatccttga ctgggccgac gagcggcgca
     1261 tcgcgtggcg ctacattcag cccggcaagc cgatacagaa cgcctttgcg gagagcttca
     1321 acgcccggct cagggacgag ctgctgaacg agacgctgtt ccgctcgctg gggcacgccc
     1381 ggcagatgat cgaagcctgg cggcacgatt acaaccacca tcgcccgcac tcgaagctcg
     1441 gctggctgac gccgaccggc tacgcgacgc gctggatgca gaacgaagag cttgaaggac
     1501 gtccatcggg ggcgttcaat gacgaccgga ttccagaccc cgctggataa aggacggggg
     1561 tcacatcaca ctcaatagca atgaatagaa atcccactat atcggcgcac tagcgtataa
     1621 tcgaaaacca attttggcac attgtggctt tccgccttcc aaccgattca ctccgaatta
     1681 tcgtctcttt ggggagatat cccaaacgcc tcttgcaagg atttcaggta ttttagtcga
     1741 ccgacaagca acgcccgacg cggtgctgct cttaggtcta accatcgcaa atgaagttcg
     1801 tgagcttgga acagcatttc gttttccgcc tccatcgcct gttgagaagc aatgcacgct
     1861 gctgagaagc cttcagtctg tgcactcatc tgctcttcaa gttcgaggat ttgtttttta
     1921 agttcatcgt caagatcgag cgctcgaccg tcagtgccaa gcctattggg aaagtcgcga
     1981 ggcgcggaag aggtgatgcg ctcctcaact gacctcataa ttgcaataat at
//
LOCUS       DASWOG010000102         6637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_222438,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000102 DASWOG010000000
VERSION     DASWOG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222438"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1119..1460
                     /locus_tag="VGB93_02050"
                     /pseudo
     CDS             1119..1460
                     /locus_tag="VGB93_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
     gene            complement(1511..1660)
                     /locus_tag="VGB93_02055"
     CDS             complement(1511..1660)
                     /locus_tag="VGB93_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144944.1"
                     /translation="MVVRVATVAFEGIEARPVDVQVHIANGNVGFTLVGLGDKAVADV
                     SLRQK"
     gene            1849..3114
                     /locus_tag="VGB93_02060"
     CDS             1849..3114
                     /locus_tag="VGB93_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144945.1"
                     /translation="MMVAASRPFADPAAAAAQLLFDLALILLMILTPVAEVFFHGPLY
                     VLLPVGACILIVAGRVAQAREDRPDFRKVLRSPITWAAVFLFFWAGLSIIWTPFPDEA
                     ATRFFKALGTAVVVFLAIISLPSKTRATNLYLLPIGLAIAACAAIIFTFFEPQIFWQG
                     EHPDATLAQRSVMSLMVLLWPALGALALREKWLLAIALAAIVTAAALATFIQVALVGL
                     ALAAVVYAIAVSEAQKVGQILGFGLAALMLLAPVAALVTFAITNLAHMAQSGPGFVFA
                     ELLLRQWPRFITGHGLGFVTSAIDTGVLPPDAPRSILFEIWYELGLLGALAFAVLAVC
                     VFNAAARMPDYIAPALLAGLVAGLVIAVWGAETTQVWWISLNGLDAIALALMCKGLPR
                     ATRPPVTLAEPGSYAPEADADFEDRNFAD"
     gene            complement(3122..3562)
                     /locus_tag="VGB93_02065"
     CDS             complement(3122..3562)
                     /locus_tag="VGB93_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY0144946.1"
                     /translation="MFYESGHWPLSTHPERGTALTERVDATPEALALIAKLKNTHGPL
                     MFHQSGGCCDGSAPMCYPRGDFRVGKSDVKLGSIGDAPFYMSESQFEYWQHTHLTIDV
                     VPGRGSGFSLESPENVRFLTRSRLFTGEEMTQLSPVESGEDTRH"
     gene            complement(3598..5118)
                     /gene="adh"
                     /locus_tag="VGB93_02070"
     CDS             complement(3598..5118)
                     /gene="adh"
                     /locus_tag="VGB93_02070"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY0144947.1"
                     /translation="MLAQSLQELKQQVALRERYDNFIGGKWVPPVKGQYFDNISPITG
                     KPFCQVARSTAEDIELALDAAHAAREAWGNTAPSQRARLLNKIADRLEGKLNLLALAE
                     TIDNGKPIRQTTAADVPLVIDHFRYFAGCILAQEGGLSEIDHTTVAYHFHEPLGVVGQ
                     IIPWNFPLLMAAWKLAPALAAGNCVVMKPAEQTPTSIMVLADIIADILPPGVLNIVNG
                     FGVEAGKPLAQNKRIAKIAFTGETTTGRLIIQYASENIIPVTLELGGKSPNIFFADVM
                     SEDDDFFDKSLEGFAMFALNQGEVCTCPSRALVQESIYDKFMEKAIKRVEKIKTGHPL
                     DPSTMIGAQASNDQLEKILSYIDIGKKEGAKVLTGGERNVLPGELADGYYVKPTVLQG
                     NNKMRVFQEEIFGPVLAVTTFKTEAEALEIANDTIFGLGAGLWTRDINRAYRMGRGIQ
                     AGRVWTNCYHLYPSHAAFGGYKKSGIGRENHKMMLDHYQQTKNLLVSYSPKALGFF"
     gene            5436..5732
                     /locus_tag="VGB93_02075"
     CDS             5436..5732
                     /locus_tag="VGB93_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0144948.1"
                     /translation="MIICSCNAISDQSVRASLTSENSPRTPGAVYRCLGCRPCCGRCF
                     ATVRAIIDETLSQSGSASAEAGAAASAAMALLDTNRDGHAQGACGQDCASCAAA"
     gene            5882..6364
                     /gene="bfr"
                     /locus_tag="VGB93_02080"
     CDS             5882..6364
                     /gene="bfr"
                     /locus_tag="VGB93_02080"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY0144949.1"
                     /translation="MQGDARVIEYLNRGLRSELTAINQYWLHYRVLDNWGYKELAKTW
                     RKESIEEMEHADRLVTRIIFLDGFPNLQVLDPLRIGQTVEEIIAADLATEKDAHRLYT
                     EAADYCFSVRDYVSKELFDHLLKDEEEHIDFLETQQELIRQVGVQLYSQKHIGELHED
                     "
     gene            complement(6374..>6637)
                     /locus_tag="VGB93_02085"
     CDS             complement(6374..>6637)
                     /locus_tag="VGB93_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated RND transporter
                     HpnN"
                     /protein_id="HEY0144950.1"
                     /translation="VAFKIYFVMACRAGTTHLLQSSLTRAVFFSAMTTATAFGSLWLS
                     HHPGTSSMGKLLAMSLVTTLCAAILFQPALMGPPRKKHGEIAN"
BASE COUNT         1259 a         1903 c         1978 g         1497 t
ORIGIN      
        1 tcatgatcct gatggcaatg atcacctggc atttgacaac cccagagcaa agccttgtcg
       61 gtcggctcgc gggcggcctc tttgtctatg cgatgtccat tccggaggcc tatcgaatgg
      121 cgaagctgcg ggtgactgga cgtctgcgga cgtaggcctg gcagcacatt tagcttccag
      181 cctgcaattc atccgtgggt cagtcatcga tggaatttcg aaagtaatcg gcgcctcagg
      241 tatttaaaat aaagggttga tgcctcaatg cgatcgatcc gacgttccca gacagacact
      301 cccgaaattg agagacgcgg gcaacgctgt gattcctccg ctaaaggaga ctgtcagctc
      361 caaatcgaaa gtaactgcac gcagaattgc gaaaggtctc tgtggttagg ggatttagtt
      421 atagccatcg gttgcttcgc agccataaca gcagcaggcc agttgccgct cacgggccct
      481 cgcctcttcg aagcaatttc gtctgcccgc atcgctaccg cggccaagcg ctcactaccc
      541 cagcgagaga ccaaggaaat tcaaagaagc ggcagcaaaa cgccggcgaa agagacgtct
      601 ctttcaaatt tgcatttgcc gacggtcact tcccttaact gagctttccg ctcatactta
      661 aagacaaggc agaatagtcg gcgcaagcgt gggtcatcag agtcaggctg tcgaacaaaa
      721 aagtcgtgaa ttcgtctttg ctcatttcgc cgcgcccatc gcgaattcgc gatgccgaat
      781 tcggcccgag ccgcaaacat gaccgtcgat cggtacgtta taaggcgggt ctgtgcagac
      841 gagatcgacg acgtcgttgc tcagaagggc gtcgtaagcc gcgctattgc acgtcgccgc
      901 ggacgagccg atggcgcccc aaaggctagg cgctccgact tggttacatg gccgccgctc
      961 ggatagtatc ccgcgctgcc gaccgacctg gctcgccacg agatgctgat gacgttcgag
     1021 ccgctatcgg ccgaggtcat ccgggagatc gtggacgaga tttttctgcc gctcgtccgc
     1081 cacgttccaa cgtttgtgta gaaacggcca ttcagcgggg aggggagcga tttatcgtct
     1141 cagggggtgg catcgatttt atgcgtcctt ggacagagaa ggtctacggc ataccccagg
     1201 agcaggtggt tggctcatac ggcttaacca gattcgaaat gggcaagaac gggatacttg
     1261 ttctccggaa gcttccaggg gtcgaattca tcgacgatgg gccaggaaag ccggtcggca
     1321 tcaaccgttt cattggtcgc cgtccggtct tcgtttttgg aaactccgac ggtgatcagc
     1381 agatgctgga atggaccgcg gcgggcgacg gtgcgcgctt cacggggatt atccatcata
     1441 ccgatgcggt gcgcgaattt acggcatagt tcgtgcgttt aaaatcaata cgttacgtgt
     1501 ctgttgagac tcacttttgc cgcaggctaa catcggcgac ggccttgtcg ccgagaccga
     1561 caagcgtgaa gccgacattg ccgttggcga tgtgcacctg cacatcgaca gggcgcgcct
     1621 cgatgccctc gaaagcgact gttgcgaccc ggacaaccat ccccgtcccc tcaatgcgtc
     1681 gctagcggtc acccaaagct aaacatatgt tcgggataac ggcaggaaca attcaagaac
     1741 atgaacgatc gaggcaaagg ccgtgctagc gtcgcattgc cgtgacggga cgtcggcgct
     1801 aatcgacagt cttcattttg ccgaagctgg cacgttgttc aggatgtcat gatggtcgcc
     1861 gcctcgagac cttttgcgga tcccgctgcc gcggcggcgc agcttctctt cgatttggcg
     1921 ctcatcttac tgatgattct gacgccggtc gcggaggttt tcttccatgg gccgctttat
     1981 gtactgttgc cggtcggcgc ctgcatcctg attgttgcag gtcgcgtcgc ccaagcacgc
     2041 gaggatcgcc cggattttcg caaggttctg cgctcgccga tcacatgggc ggccgtattc
     2101 ctttttttct gggcgggcct gtcgatcatc tggaccccgt ttcccgacga ggctgcgacg
     2161 cgtttcttca aagcgctcgg caccgccgta gtggtgttcc tggcgattat ctcactgcca
     2221 tcgaaaacgc gggcgacgaa tctctatttg ttgcccatcg gccttgcgat cgcggcctgt
     2281 gcggcgatca tattcacctt cttcgagccg cagatattct ggcagggcga acatcctgac
     2341 gcgacgctgg cgcagcgcag cgtgatgtcg ctgatggttt tgctgtggcc ggcacttggt
     2401 gcattggcgc tgcgggaaaa atggcttctc gcgattgcgc tggcggcaat cgtcacggcg
     2461 gcggcgctcg cgactttcat ccaggttgcg ctcgtgggtt tagccttggc cgccgtggtt
     2521 tatgcgattg cggtcagcga agcgcaaaaa gtcggacaga tcctcggctt cggacttgcc
     2581 gccttgatgc tgcttgcgcc ggtcgcagcg ctggtgacat ttgccattac gaatctcgct
     2641 catatggccc agagcggtcc cggttttgtc tttgctgagc ttctgttgcg gcaatggccg
     2701 cggttcatca cggggcatgg tctcggtttc gtgacatctg ccatcgacac tggcgtgctc
     2761 ccgccggatg ccccgcgcag cattctcttt gaaatttggt acgaacttgg cttgcttggc
     2821 gcgctcgcgt ttgcggttct cgccgtctgc gttttcaacg ccgccgcccg catgcccgat
     2881 tacatcgcgc ccgccttgct cgccggcctc gtcgccggcc tcgtgatcgc cgtctggggc
     2941 gcggaaacga cgcaggtctg gtggatatcc ctgaatggcc tcgacgctat tgcgctcgca
     3001 ctgatgtgta aggggctgcc gcgcgccacc cggccgccgg tgacgctcgc ggagcccggc
     3061 tcttatgcgc cggaggctga cgcggatttt gaagaccgca attttgccga ttagcgcgtg
     3121 cttagtggcg cgtatcttcg ccgctttcga cgggtgacaa ttgcgtcatc tcttcgcccg
     3181 tgaagaggcg gctgcgggtg agaaagcgaa cgttctcagg cgattccagc gaaaagcccg
     3241 agccccggcc tggcaccaca tcaatcgtca gatgcgtgtg ctgccaatat tcgaattgcg
     3301 attcgctcat gtagaagggc gcatcgccga tgctgccaag tttcacatcg cttttgccga
     3361 cgcgaaaatc gccgcgcgga taacacatcg gcgccgagcc gtcgcagcag ccgcccgatt
     3421 gatggaacat caaagggccg tgggtattct tgagtttggc aatgagggca agagcctcag
     3481 gtgtagcatc gacacgttca gtcaaagctg ttcccctttc cggatgcgtt gagagcggcc
     3541 aatggccgct ctcgtagaac attgagacgc cgaggtccgg cgtcaagccg taaacgatca
     3601 gaagaagccg agcgcctttg ggctgtagct caccaggagg tttttcgtct gctgataatg
     3661 gtcgagcatc atcttgtggt tctcacgccc gatgccggat ttcttgtagc caccgaaggc
     3721 cgcatgcgac ggatagaggt ggtagcaatt ggtccagacg cggccagcct ggataccgcg
     3781 gcccatgcga taggcgcggt tgatgtcgcg cgtccacagg ccggcaccga ggccgaagat
     3841 cgtgtcgttg gcgatttcga gcgcttcggc ctcggtcttg aacgtcgtga cggcgagaac
     3901 cgggccgaag atttcttcct ggaagacacg catcttgttg ttgccttgga ggaccgtcgg
     3961 cttgacgtaa tagccgtccg cgagttcgcc gggcagcacg ttgcgttcgc cgccggtcag
     4021 aaccttcgcg ccttccttct tgccgatgtc gatgtaggag aggattttct ccaattgatc
     4081 gttcgacgcc tgtgcaccga tcatggtcga aggatcgagc gggtggccgg tcttgatctt
     4141 ctcgacgcgc ttaatcgcct tttccatgaa cttatcatag atcgattcct gcacaagcgc
     4201 gcggctgggg caggtacaga cttcgccctg gttcagcgcg aacatagcga aaccttcaag
     4261 cgacttgtcg aagaaatcgt catcttcgga catgacatcg gcaaagaaga tgttcggcga
     4321 tttgccgccg agttccagag tcaccggaat gatgttttcc gacgcatatt gtatgatgag
     4381 ccggcccgtc gtcgtctcgc cggtgaaggc gatcttggcg atgcgtttgt tctgcgccag
     4441 cggcttgcca gcctcgacac cgaaaccgtt gacgatgttg agcacgccgg gcggcaggat
     4501 gtcggcgatg atgtcggcga gcaccatgat gctggtcggg gtctgctcgg ccggtttcat
     4561 cacaacgcaa ttgcctgcgg cgagagccgg ggcaagcttc caggctgcca tcagcagcgg
     4621 aaagttccac gggatgatct gtccaacgac gccgagtggc tcatggaaat gataggcaac
     4681 cgtggtgtgg tcgatttcag agaggccgcc ttcctgcgcg aggatacagc cagcaaaata
     4741 gcggaaatgg tcgataacga gcggcacgtc ggcggcggtc gtctggcgga tcggcttgcc
     4801 gttatcgatc gtttcggcga gcgcgagcag gttgagtttg ccctcaagcc ggtcggcgat
     4861 cttgttcagg aggcgggcgc gctgcgacgg cgcggtattg ccccacgcct cgcgggcggc
     4921 gtgcgcggcg tccagcgcca gttcgatgtc ttcggcggtt gagcgcgcca cttggcagaa
     4981 gggcttgccg gtgatcgggc ttatgttgtc gaagtattgg cccttaaccg ggggcaccca
     5041 cttgccgccg atgaaattgt catagcgttc gcgaagagcg acctgttgct ttagttcctg
     5101 aaggctttgc gcgagcatat ctggatctcc ctatctagcg tcttttgttg gcgtcattat
     5161 cgcgccgtat gccggggtga ggcaataggc ttgcggtctt gggtggagcc ccgagccaag
     5221 ctgcgggggc tgcatttcag tttcgttttt cagcgagacg agttgttttg ctcaggccag
     5281 tttatacttg ttttaaacta gccgccttcg ctctttttaa cgactataaa gttggtctcc
     5341 ttctcttgcg ccgaagccgg ttcgccgcca ttaccttgag aagacacacg ttgtcgcttg
     5401 gaagcccgcc gcgacccttg agctgaatca aaccgatgat catttgttca tgcaatgcga
     5461 tttcggatca aagcgtaagg gcttccctca ccagcgaaaa ctccccacgc acgcccggtg
     5521 cggtctatcg ctgtcttggc tgccgtccgt gttgcggacg ttgctttgcc acggttcgcg
     5581 cgatcattga tgaaacgttg agccagtcgg gctccgcctc cgccgaggct ggggccgcgg
     5641 ccagcgcggc catggcgctt ctcgacacca accgggatgg ccatgcgcaa ggcgcttgcg
     5701 gccaggattg cgcaagctgc gctgcggcct gaattccggc tggcattctc tacagccata
     5761 gccgaatcct ttcctcatct gcccctggtc tccgcttttg tgccatcgct cgggctattg
     5821 cctgtccatg ctggctgccc tagggttccg ccggtcattc ctagaataga gagattctaa
     5881 aatgcagggc gacgcgcgcg tcatcgaata tttgaatcgc ggccttcgga gcgaactcac
     5941 cgcgatcaac caatattggc tgcattaccg cgtgctcgac aattggggct acaaggaact
     6001 cgccaagacg tggcgcaagg aatcgatcga ggagatggaa cacgccgacc gtctcgtgac
     6061 gcgcatcatc tttcttgatg gctttcccaa tctccaggtg ctcgatccgc tgcggatcgg
     6121 ccagacggtc gaggagatca tcgccgccga tcttgccacc gaaaaggacg cgcaccggct
     6181 ctataccgaa gccgccgatt attgcttctc cgttcgcgac tatgtctcga aagagctttt
     6241 cgaccatctc ctcaaggacg aggaggaaca catcgacttc cttgagaccc agcaggaatt
     6301 gatccgccag gtcggcgtcc agctttattc acagaagcat atcggcgaat tgcacgaaga
     6361 ctgaggcttc gtctcagttc gcgatttcgc catgcttctt gcgcggtgga cccatgaggg
     6421 cgggctggaa aagtatcgcc gcgcagagtg ttgtcaccag cgacatcgcc agcagcttgc
     6481 ccatgctcga cgtgcccgga tggtgcgaaa gccacaggct gccgaaggcg gtggccgtcg
     6541 tcatcgcgct gaagaagacc gcgcgtgtca ggctcgattg caacaggtgc gtcgtgccgg
     6601 cacgacaggc catcacgaaa taaattttaa atgccac
//
LOCUS       DASWOG010000103         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_185838,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000103 DASWOG010000000
VERSION     DASWOG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185838"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VGB93_02090"
     CDS             <1..562
                     /locus_tag="VGB93_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0144951.1"
                     /translation="LGTIANIETVAQRLAIHKPAYVVLDPVTRASSGDALATEDLAAA
                     LIDHLAPLTTLVTPNLAEAAKLAQDQVPTSIEQMSAIAAKLHRAGFSAVLVKGGHLAG
                     AESVDLLCDADAERVFTAPRVATKNTHGTGCTLSAAIAASLANDLPLAAAIEAAKAYV
                     SASLAGADSLSVGHGAGPLHHFRDLW"
     gene            684..1115
                     /gene="mscL"
                     /locus_tag="VGB93_02095"
     CDS             684..1115
                     /gene="mscL"
                     /locus_tag="VGB93_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0144952.1"
                     /translation="MWKEFRDFAMRGNVVDLAIGVIIGAAFGKIVDSLVNDMIMPLIG
                     AITGGLDFSNYFLPLSHHVTSNVLVDAKKQGAVLAWGSFATIVINFLIVAFVLFLVVR
                     GINMLRARVVQEKKVEEAAAPPSKEEVLLTEIRDLLAKGVK"
     gene            complement(1169..1798)
                     /locus_tag="VGB93_02100"
     CDS             complement(1169..1798)
                     /locus_tag="VGB93_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0144953.1"
                     /translation="MKSRFPFVLVLAVLAGCGLTSLAINAYADPVSGQTTEQPSSSDA
                     SSSDAPEHFHFSDADRAAFLDARIAALHAGLVLTPAQEKLWPSVESALRETHKVIADQ
                     RAARRNSPRPADPVAWLQRASDNTMARGEALKKLADAAGPLYSSLNDDQKNRLPFLLH
                     AAHFHFFGARFAANDNWHGQHDWRGDRGGDDSQGRDDEADHAPDGDNSQ"
     gene            complement(1899..3698)
                     /locus_tag="VGB93_02105"
     CDS             complement(1899..3698)
                     /locus_tag="VGB93_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144954.1"
                     /translation="MTEVATKTLPAATQSKPLPVSILVFAALALGLVMSSADLHSVWQ
                     HGTFADSDDAMQLVEVRNFLAGQNWFDLTVARLDPPYGVFMHWSRFIDAPLALLIKTF
                     ELFMPADAAERLTRICFPLLLQGLLYVGIARLARQLAGKAVILPAIVLTLLSGMTYTQ
                     FQLGRINHSASQIVLLIFMLGSFIDALDPVRAWRAAFVGILAAVSLAISLENLPWIFV
                     FAALAIAFFVVRGAEMRTHLIAFGAGLGIALPLTYVTTIGPARWLDKSCDAYSLVYFM
                     PGLAGAAMLVFLGAASPWFKSAPARIGVAFAATLCVGGAVALGKPICFLDPYAGIDPL
                     LRDVWLKNVEEALPFSRYFVMRPSAAIIMLMPVLLGFAAVIVACRVEQGLARLRWLTI
                     ASLTAAGLLMCFWQIRIFSFVGPVALLGAAWAIARWRDRLLRSRWHEASALALILVLP
                     FSSIGWALAAMGFPSPAQKQEHARTACLASSAFVPLAQLPPGLVAGPIDAGSHILALT
                     PHSVLAAPYHRDNHGNRVALDAFLAPPAQAQSILRANNVQYVVNCTGFGEMKVLAKRA
                     PNSLAMHIVANTPPAWLVPSPSSGAYQVFVIRP"
     gene            3889..4317
                     /locus_tag="VGB93_02110"
     CDS             3889..4317
                     /locus_tag="VGB93_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144955.1"
                     /translation="MPPIRQPSFADVLEDVALAPHEEFVEDEGSSAPVNQDWLFHIFI
                     EAAEAVATPRRPDAYRENASDVFQMPLFDATADEDSIAAELDLPAAKNLDDLARIRRG
                     FARRNHPDKLHPRLAEIATARMKTANMLIDRREHELKQRS"
     gene            complement(4352..4909)
                     /locus_tag="VGB93_02115"
     CDS             complement(4352..4909)
                     /locus_tag="VGB93_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0144956.1"
                     /translation="MKADLIAVIPNLRAFAVSLCGNPDRADDLVQETLVKAWGNLDSF
                     VEGTNLPAWLFTILRNFYYSEYRKRRREVADPDGIIATKLATAPAQNGHMDLRDLHDA
                     LQKLPGDQREALILIGASGLSYEEAANICGCAIGTMKSRVNRARNRLAAILAIHSTSE
                     LGHEEDWPLNDPMAVSHHLSKTGSD"
BASE COUNT         1094 a         1441 c         1496 g          893 t
ORIGIN      
        1 gttgggcacg atcgccaata tcgaaacggt ggcacagcgg cttgcgatcc ataagcctgc
       61 ttatgtcgtg ctcgatccgg tgacgcgcgc ctccagcggc gacgcactcg ccacggaaga
      121 tctcgccgcg gcgctgatcg atcatctcgc gccgctgacg acgctggtga cgcccaatct
      181 cgccgaggcg gcgaagcttg cccaagacca ggttcccacc tcgatcgaac agatgagcgc
      241 gatcgccgcc aagctgcacc gggccggatt cagcgccgtc ctcgttaaag gcggtcatct
      301 tgcgggtgcc gagtctgtcg atttgctatg cgacgcggat gctgagcgcg ttttcaccgc
      361 acctcgcgtc gccaccaaaa atacgcatgg caccggctgc acgctgtctg cggcgatcgc
      421 tgcgagcctc gccaacgatc ttccgctcgc cgcagcgatc gaagccgcga aagcctatgt
      481 cagcgcgagc ctggcgggcg ccgatagtct gagcgtgggc cacggcgccg ggccgcttca
      541 tcattttcgt gacctctggt aactagccgg aggcgcagcg tgcgatgcag cacttcgggc
      601 atactttcca gacccgcata acaaaacgca tccaaacgca aacgcgtagg gttctaaggc
      661 ggttttcaaa agggggtgag agcatgtgga aggaatttcg tgacttcgca atgcgcggca
      721 atgtcgtcga cctcgccatc ggtgtgatca ttggcgcggc cttcggcaag atcgtcgatt
      781 cgctggtcaa tgatatgatc atgccgctca ttggcgcgat cacgggcggc ctcgattttt
      841 ccaactattt cctgccgctg tcacatcatg tgacgtcgaa tgtgctggtc gatgcgaaaa
      901 agcagggcgc cgtgctcgcc tggggtagtt tcgcgacgat cgtgataaat ttcctgattg
      961 tcgccttcgt gcttttcctg gtcgtccgcg gcatcaatat gctgcgcgcc agagtcgtgc
     1021 aggaaaagaa agtcgaagaa gccgcggcac cgccgtcaaa ggaagaagtt ctgctgacag
     1081 agatccgcga ccttctcgcc aagggcgtaa aataatccgg cggaatgcct tcgcccgtgc
     1141 gggcgaaggc aaattataaa gttcagactt actgagaatt gtcgccgtca ggcgcgtgat
     1201 cggcttcgtc gtcgcgacct tgcgaatcat cgccgccgcg gtcaccgcgc caatcatgtt
     1261 ggccatgcca attgtcattg gcagcaaagc gcgcgccaaa gaaatgaaaa tgcgcggcgt
     1321 gcagcagaaa cggcagccgg tttttctgat cgtcattgag cgatgaatac aagggcccgg
     1381 cggcgtcagc caatttcttc aacgcttcac cacgcgccat cgtgttgtcg ctggcgcgct
     1441 gcagccacgc gaccggatcg gcagggcgcg gactattgcg gcgtgcggcg cgctgatcgg
     1501 cgatgacttt atgcgtctca cgcaaggcgc tctctactga cggccagagc ttttcctgcg
     1561 cgggcgtcaa tacgaggccg gcatgaagtg ctgcgatccg cgcatcgaga aaagccgcgc
     1621 gatccgcatc ggaaaaatgg aaatgctctg gagcgtcgct cgatgaggca tcgcttgacg
     1681 agggttgctc cgtcgtctga cccgagaccg gatcggcgta agcgttgatg gcaagagacg
     1741 taagcccgca accggcaagg acggcaagga cgagaacaaa tggaaagcgc gatttcattg
     1801 atgacctccc ttcgacgcga atcgatcaaa gagagctagg ctcgcgctgc gctgccttca
     1861 aattaagctt ttgcaatctc gcggcgaaac gcctgcgatt aagggcgaat cacgaagact
     1921 tgatacgcgc cgctagacgg cgagggcacc agccaggccg gcggcgtatt cgcgacgatg
     1981 tgcatcgcaa gagaattggg tgcgcgcttc gctaacactt tcatttcacc gaacccggta
     2041 caattgacga catattgcac gttgttcgcg cgcaggatgc tttgtgcttg agccggcgga
     2101 gcgaggaagg catcgagcgc aacgcgattg ccgtgattgt cacgatgata cggcgccgcc
     2161 agtaccgaat gcggcgtcag cgccagtatg tgcgagccgg catcgatcgg tccggcgacc
     2221 agccccggcg gcaattgcgc gagtggtacg aaggcggatg aggcgaggca ggccgtgcgc
     2281 gcgtgctcct gcttttgcgc agggctcgga aaacccatcg ccgcaagcgc ccatccaatc
     2341 gacgaaaacg gcaggacgag gatgagcgcg agcgccgagg cctcatgcca gcggctccgc
     2401 aaaagacggt cgcgccaacg ggctatcgcc caagcggcgc cgagcaatgc gaccggtccg
     2461 acgaaactga aaatgcggat ctgccagaag cacatgagca agccggccgc ggtgagcgaa
     2521 gcgattgtca gccatcgcag acgggcgagg ccctgttcca cgcggcaagc cacgatcacc
     2581 gccgcaaagc caagcaaaac aggcatcaac ataatgatgg ccgccgaagg ccgcatgacg
     2641 aaatagcggg agaacggcaa ggcttcttcg acgtttttca accagacatc gcgcaggagc
     2701 ggatcgatcc cggcataggg atcgagaaag cagatcggct tgccgagtgc gaccgcgcca
     2761 ccaacgcata gagtggcggc gaaagcgacg ccgatgcgcg ccggcgcgct tttgaaccaa
     2821 ggcgaggccg cgccgagaaa aacaagcatc gcggcacctg cgaggcccgg catgaaatag
     2881 acgagcgaat acgcatcgca gcttttatcc agccaacgcg ccggtccgat cgtcgtgacg
     2941 tacgtgagcg gcaacgcgat accgagaccg gcgccgaatg cgatgaggtg tgtccgcatc
     3001 tccgcgccgc gaaccacgaa aaatgcgatg gcgagggcgg cgaagacgaa aatccagggc
     3061 agattttcga ggcttatcgc aagcgagacg gcggcaagaa tgccgacaaa agccgcccgc
     3121 cacgcgcgca ccggatcgag agcgtcgata aaactgccga gcatgaagat gaggagaaca
     3181 atttgcgagg ccgagtgatt gatgcggccg agctggaatt gcgtataggt catgccgctc
     3241 agcaaggtaa ggacaatcgc gggaagaatc accgcttttc ctgcgagttg gcgcgcaagt
     3301 cgcgcaatgc cgacatagag caggccctgc agcaagagcg gaaaacaaat gcgcgtcagc
     3361 cgctcggcgg catcggctgg catgaagagt tcaaaggtct tgatcaggag cgcgagcggc
     3421 gcatcgatga atcgcgacca atgcatgaag acaccatacg gcggatcaag ccgcgcgacc
     3481 gtgaggtcga accaattctg tccggcaaga aaattgcgaa cttcaacgag ctgcatcgcg
     3541 tcgtctgaat cggcaaacgt accatgctgc catacgctgt gaagatcggc ggatgacatc
     3601 acgagcccca gcgccagggc tgcgaagacc agaatcgaaa ccggtaaagg cttgctctgc
     3661 gtcgccgccg gcagcgtttt ggttgcgact tcggtcatct ccatgcttgc cacatcgcgc
     3721 tcttttgaat ccgccgaaca aatcttgtgc gacaaaagct tttacgccgc gttacggaag
     3781 gcggggattt tgcggacgcg tactccggcg gcgctcggaa cacggcgtag ttaattcaat
     3841 cttaagcgcc aatgggttga atgcgcccgg aatgagctgg agtttctcat gccgccgatc
     3901 aggcagccgt ctttcgccga tgtcctcgaa gatgtcgcac tcgcgccgca tgaagagttc
     3961 gtcgaggacg agggctcctc ggcccctgtc aatcaggatt ggctgtttca tattttcata
     4021 gaagctgccg aagccgtcgc cacaccgaga cgcccggacg cttatcgcga aaatgccagc
     4081 gatgtctttc agatgccgct cttcgatgcg acagccgacg aagacagcat cgcggctgag
     4141 cttgatctcc cggccgcgaa aaatcttgac gatcttgccc gcatccgtcg cggtttcgcg
     4201 cggcgcaacc atccggacaa gctgcatcca cgcctcgcag aaatcgcgac agcgcggatg
     4261 aaaaccgcca atatgctcat cgaccggcgg gagcacgaat taaagcagcg cagctaaggc
     4321 gcgctttgcc gttcaaatga tggcgaaaac gctaatcgct acctgtcttc gacaggtgat
     4381 ggctgacggc catcggatcg ttgagcggcc aatcttcctc gtggcccaat tcgctcgttg
     4441 aatggatcgc gaggatcgct gcgagacggt tgcgcgcgcg attgacgcgg ctcttcatcg
     4501 tgccgatcgc gcagccgcag atgtttgccg cttcttcgta tgaaaggccc gaagcgccga
     4561 tcaggatcaa cgcttcgcgc tgatcacccg gcaatttctg caaggcgtca tgtaaatcgc
     4621 gcagatccat atggccgttc tgcgccggcg ccgttgcgag cttcgtcgcg attatgccgt
     4681 caggatcggc gacctcgcgc cggcgtttgc gatattcgga atagtagaaa ttgcgcagaa
     4741 tggtgaaaag ccacgcaggc agattggtac cctcgacgaa agagtcgaga ttgccccacg
     4801 ccttaacgag cgtttcctgc accagatcgt cggctcggtc cggatttccg caaagagaaa
     4861 ccgcgaaagc acgcaaattc ggaatgaccg cgatcagatc cgctttgatc tgcgcgctgg
     4921 cctg
//
LOCUS       DASWOG010000104         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_167388,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000104 DASWOG010000000
VERSION     DASWOG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167388"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VGB93_02120"
     CDS             <1..1338
                     /locus_tag="VGB93_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0144957.1"
                     /translation="SPGEDTAFTENGNSDFNSSTLNENQVERTFYNVASWQKSAGNID
                     AQIAFFSRYSTLHFVPDEVGDLLFNGVASDVQRNSLLNGFQGDAAYHLSAHTIRFGFE
                     VSGESTDAVDNDLVLPASGSSAPFSVDNSSNKLGWLGSFYTQDEWRITNQLTINGGLR
                     FDQMAQYVTTNQLSPRLSLTYKPFESTTFHAGYARYFTPPEQVLSAPTNLGAFANTSA
                     ASAVNEASPVRPERSDYFDAGVTQKLFPGLEAGVDAYYKRARNLLDDGQFGQALVLTA
                     FNYAKAYNTGVEFKTNYQVGDFSAYGNFAWARQRATQVSSNQFLFDPDELAFIAGHYI
                     YTDHAQTLTASVGASYLWQATKFSADFIYGSGLRDGFANTGTVAPYTQVNLGVSHEFQ
                     LPGNSGLGPLEARFDVVNLFDNIYEIRDGSGIGVFAPQFGPRRGFFGGLTQKF"
     gene            1393..1866
                     /locus_tag="VGB93_02125"
     CDS             1393..1866
                     /locus_tag="VGB93_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY0144958.1"
                     /translation="MPLYFAYGANMDPNAMRLRAPNSRALGRARLARHKFFVMKSGFA
                     SVRRDPHADVHGVLFDLALSDVLALDRYEEVGRGLYTKVVQPVLREGAAAARALVYVG
                     ASTEEGKPAAAYLDGILTGAKENGLPENYIAFLASFGGPQAGPGTRWRAIKAEGI"
     gene            1868..2713
                     /gene="panC"
                     /locus_tag="VGB93_02130"
     CDS             1868..2713
                     /gene="panC"
                     /locus_tag="VGB93_02130"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0144959.1"
                     /translation="MTVEIARDVASLRAFVMNWHAAGTRVALVPTMGALHEGHLSLVA
                     DAKRRADRVVMSIFVNPAQFAPNEDFAAYPRTIQTDVEAFIAAGGDLVFAPEIGEVYP
                     PDFVTKISLGGPATAGLEDKFRPDHFTGVATVVAKLLIMAKPDVALFGEKDYQQLAVI
                     RQMARDLNLESEIVGVQTVRAKDGLALSSRNVYLTPEERARAPLLHGALERCRKAILA
                     GEEIAVAIARAEGALTMMDFAVDYVAACHAVTLAPVTSAADRPIRLLAAARLGATRLI
                     DNIAV"
     gene            2847..3746
                     /locus_tag="VGB93_02135"
     CDS             2847..3746
                     /locus_tag="VGB93_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0144960.1"
                     /translation="MRIGVDIGGTKIEAVALADDGKIIDRRRKPTPTGDYQSILDVVS
                     SLARGLEADMGETATVGVAMPGTISPGTGLMRYSNALVMNGKPFANDIGEVLGREVRT
                     ENDANCLALSEAVDGAAMGAACVFGIILGTGVGGGLVIDRKLVPGRNRIAGEVGHIAL
                     PLNEVERRMPPRRCFCGQDDCVETFLSGGAFSAEHRQRTGKDMSATSIAEAAANGDGA
                     AQATLEVYVDRLARALAVVMKIVDPDVFVFGGGVSKIPQLYDLLPPLLARYVYSDVMT
                     TRVLQAGHGDSSGVRGAAWLWPR"
     gene            complement(3743..>4097)
                     /locus_tag="VGB93_02140"
     CDS             complement(3743..>4097)
                     /locus_tag="VGB93_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEY0144961.1"
                     /translation="ALESSYFSVRAVLAKSVSEAARFVVNRGLADEGAPVIVRLISQI
                     AARFGVVVSQKVAAQAVPIIGAAGGAAVNYAFIDHFQSIAEGHFTVRRLERAYGPEIV
                     RAEYDRLLPETRAEN"
BASE COUNT          770 a         1219 c         1257 g          851 t
ORIGIN      
        1 tcacccggcg aggacacggc ctttacggag aacggcaata gcgatttcaa ttcgtcgact
       61 ctgaatgaaa atcaggtcga acggacattc tacaacgtcg cttcctggca gaaatcggct
      121 ggtaatatcg acgcgcaaat cgccttcttc tcgcgctatt caacactcca tttcgtcccg
      181 gacgaggttg gcgatctgct gttcaatggt gtcgcgtcgg atgttcaacg caacagcctg
      241 ctaaacggtt tccagggcga tgcggcctat cacctcagcg cgcataccat ccgtttcggc
      301 ttcgaggtca gcggtgaatc gacggacgcg gtcgataacg atctcgtcct tccggcaagc
      361 ggctcgagcg cgccgttctc ggttgacaat tcgagcaaca aactcgggtg gctgggctcc
      421 ttctacacac aggacgaatg gcgcatcacg aatcagttga cgatcaatgg cggcctgcgt
      481 ttcgatcaga tggcgcaata tgtcacgacc aatcaattga gcccgcgtct tagcctgact
      541 tacaagccct tcgaatctac gacatttcat gccggctacg cgcgctattt cacgccgccg
      601 gagcaggtgc tgtcggcgcc gactaatctt ggcgccttcg caaacacatc agccgcatcg
      661 gcggtcaacg aggcgagccc tgtgcggccg gagcgttcgg attatttcga cgcaggcgtg
      721 acgcagaagc tctttcctgg cctcgaagcc ggtgtcgatg cctattacaa gcgggcgaga
      781 aacctgctcg atgatgggca attcggccag gcgctggtgc tgacggcttt caattacgcg
      841 aaggcctaca acaccggcgt cgagttcaag acgaattatc aggtcggtga tttctcggct
      901 tatggcaatt tcgcctgggc gcggcagcgc gcaacgcaag tctcatcgaa ccaattcctg
      961 ttcgatcccg acgaactcgc ctttatcgct ggtcattata tctacaccga ccacgcgcaa
     1021 acgcttacag cttcggtggg cgcgtcctat ctttggcagg cgaccaaatt cagtgccgat
     1081 ttcatctatg gcagcggctt gcgtgatggt ttcgcaaata cgggaacggt cgcgccttat
     1141 acgcaggtga acctcggcgt ttcgcatgaa ttccaattgc cgggaaattc cggccttgga
     1201 ccgctcgaag cgcgcttcga tgtcgtcaat ctcttcgaca acatctacga gatcagggac
     1261 gggtcgggca tcggcgtctt cgcgccgcag ttcgggccgc ggcgtggctt tttcggcggc
     1321 ctgacgcaaa aattctgaca aaggctggtc gggtggatta taggtccgga cgctttttgt
     1381 ctggacccgc atatgccgct ttattttgcc tatggcgcca acatggaccc caatgccatg
     1441 cgtctgcgcg caccaaattc gcgcgcgctg ggccgggcgc gtctggcccg acataagttc
     1501 ttcgtcatga aatcgggttt cgcctcggtt cggcgcgatc cgcatgctga tgtgcatggc
     1561 gtgctcttcg atctggcctt gtccgatgtg ctggctctcg atcgttacga agaagtcggc
     1621 cgtgggctct acacaaaagt cgtccagccg gttttgcgcg aaggcgcggc ggcagcgcgc
     1681 gcgctcgttt atgtgggcgc gagcacggag gagggcaaac ccgcggcggc ttatctcgat
     1741 ggcattctga ctggcgcaaa ggaaaacggc ctgccggaaa attacatcgc gtttctcgcg
     1801 tcgttcggtg gcccacaagc cggaccgggc acacgttggc gcgcgatcaa ggcagagggt
     1861 atttgaattg accgtcgaga ttgcgcgcga tgtggcatcg ctccgcgcct ttgtcatgaa
     1921 ttggcacgcg gcaggcacgc gtgtcgctct ggtgccgacg atgggcgcct tgcacgaggg
     1981 acatctatcg ctggtggcgg acgcgaagcg gcgtgccgat cgcgtcgtga tgtcgatctt
     2041 cgtcaacccg gcgcaattcg cgccgaacga agatttcgcg gcctatccac ggacgatcca
     2101 gacggacgtc gaagctttca ttgctgctgg cggcgatctc gtattcgcgc cggaaattgg
     2161 ggaagtctat ccgccggatt tcgtcaccaa gatcagcctg gggggcccgg caacagcggg
     2221 ccttgaggat aaattccggc cggaccattt cactggtgtt gcaacggtgg ttgcgaaact
     2281 gctgatcatg gccaagcccg acgtcgcgct tttcggcgag aaagattacc agcagctcgc
     2341 agtcatccgt cagatggcgc gcgatcttaa tctcgaatcc gaaatcgttg gtgtgcaaac
     2401 tgtgcgtgcc aaggatggtc tcgcgctctc gtctcgcaat gtctacctca cgcccgagga
     2461 gcgtgcgcgg gcgccgctgc ttcatggcgc gcttgagcgc tgccgcaagg cgatcctcgc
     2521 gggagaggaa attgccgtgg cgatcgcgcg cgccgaggga gcgctgacaa tgatggattt
     2581 cgccgtcgat tacgttgcgg cgtgtcacgc ggtgacgctc gcgccggtca cttccgcggc
     2641 ggatcgaccg atccggctgc tcgccgccgc gcgcctgggg gcgacgcggc tcattgacaa
     2701 tatcgctgtg taaatagggc cctgcagccg ttttctgcta cgggcgacga cggcaaagct
     2761 tgcttgcgga taaagccggc ttatcacttt cccaaagcct gtcgcgggcc atatagaccg
     2821 gataaatccg gccaagcgga ggaaacatgc gaatcggcgt cgatatcggc ggcacaaaaa
     2881 tcgaggcagt tgcgcttgcc gatgacggca agatcatcga ccgccggcgc aagccgactc
     2941 ccaccggcga ctatcaatcg atcctcgatg tggtcagttc gcttgcgcgc ggtctcgaag
     3001 ccgatatggg cgagacggcg acagttgggg ttgcgatgcc cggaacgatt tcgccgggca
     3061 ctggcctcat gcgttattcc aacgcgctgg tgatgaatgg caagccgttc gcaaacgata
     3121 ttggcgaggt tctcggccgc gaagttcgaa ccgagaacga tgcgaattgt ctggcactat
     3181 ccgaggcggt ggacggcgcg gctatgggcg ccgcctgcgt cttcggaatc attctcggca
     3241 caggtgtcgg cggcggtctt gtcatcgacc gcaaactcgt ccccggccgc aacaggatcg
     3301 ccggtgaagt tggccatatt gcgctgcccc tcaacgaggt tgagcgccgc atgccgccgc
     3361 gccgctgctt ctgcggccag gacgattgcg tcgaaacctt tttatccggc ggcgcttttt
     3421 ctgcggaaca tcgccaacgg acgggcaagg acatgagtgc cacctcgatc gccgaggcgg
     3481 cggccaacgg tgatggcgcc gcgcaggcca cgctcgaagt ctacgtcgat cgtctggccc
     3541 gggcgctggc cgtcgtgatg aaaatcgtcg atccggatgt gttcgtcttt ggcggcggtg
     3601 tctcaaagat cccgcaactt tacgatcttc taccgccgtt gctcgcgcgt tacgtctatt
     3661 cggacgtcat gacgacgcgc gtgttgcagg ccgggcatgg cgattcgagc ggtgtgcgcg
     3721 gcgccgcctg gctctggccg cgctaatttt ccgcgcgcgt ttctggcagc agccgatcgt
     3781 attcggcgcg gacaatttct ggcccgtatg cgcgttcgag ccgacgcacg gtaaaatgcc
     3841 cttcggcgat cgattggaaa tgatcgatga aggcataatt gaccgctgcg ccaccggccg
     3901 cgccgatgat cggaacggcc tgcgccgcga ccttctgcga gacgactacg ccaaagcgcg
     3961 ccgcgatctg cgagatcagc cggacgatca ccggcgcgcc ttcgtcggcg aggccgcgat
     4021 tgacgacaaa acgcgccgcc tcggagaccg atttggcgag cacggcccgc acggaaaaat
     4081 agctactctc cagcgcc
//
LOCUS       DASWOG010000105         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_259159,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000105 DASWOG010000000
VERSION     DASWOG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259159"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGB93_02145"
     CDS             <1..235
                     /locus_tag="VGB93_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEY0144962.1"
                     /translation="VTGPADEARALAEKLPSKTSRDYGEPFSDIFKKFKGDFYKIDPM
                     LFSPAVAVVTAVESGETFFGGEINESALNASFS"
     gene            219..1148
                     /locus_tag="VGB93_02150"
     CDS             219..1148
                     /locus_tag="VGB93_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEY0144963.1"
                     /translation="MPPSLEAGPRIALLVDKIEFHAKELTRAFAKLGARAVPMRLSAF
                     SLDTRRPSGIAVPGFGTRLPDLVLVRAVGLGTFESTTLRLGLLHALVGMGVPVANSPR
                     SIELCVDKAATSFRCFNQGVATPETFALESPRQTRALVRRESSRGPLVLKPLFGAQGR
                     GLKLIRHESDLPDIAEMAGVYYLQRYIGVERDGFSDCRALVAGGRVLAAMTRRADHWI
                     TNVKLGAKPEPVTLDDEMRDLAVRAAAAVEAEFAGVDLLRGADGRWYVIEVNSMPGWQ
                     GLQSVTDFSIADELAALLLARHGLAAGPAEMVS"
     gene            1145..1993
                     /locus_tag="VGB93_02155"
     CDS             1145..1993
                     /locus_tag="VGB93_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEY0144964.1"
                     /translation="MIAAAEIAAAFRAACREEIEAPKPGNVHIFAGGHNMEAAHFLMS
                     ADVSAGPIAAGASVGARILAAVEASFAAVGMNTNLGIILLCAPLAAAAERRGDLRRAL
                     ADVLVGLDREDAAHVFRAIALASPGGLGDAARYDVRKPADVDLMEAMREAAPRDRIAY
                     QYASNFDDIFVTGFASLEDAKEAGINPPLRAVNVYLSFLSRFPDTHIFRKFGADTAAA
                     VQQEAREILPLFKARPHDCLTDLLAFDARLKARGFNPGTSADLTVATLFVDRLRNGLP
                     IRRNDG"
     gene            2169..>2264
                     /locus_tag="VGB93_02160"
     CDS             2169..>2264
                     /locus_tag="VGB93_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEY0144965.1"
                     /translation="MAKITKLGVGESLVGEGNEVAHIDLIIGPRGS"
BASE COUNT          387 a          690 c          718 g          469 t
ORIGIN      
        1 cgtcaccggc ccggctgacg aggcacgtgc actggccgag aaactgccga gtaagacctc
       61 gcgcgattat ggcgagccct tctccgacat cttcaagaaa ttcaaaggcg atttctacaa
      121 gatcgatccg atgctgttca gccccgcggt cgcggtcgta acggcggtcg agagcggcga
      181 aaccttcttc ggcggcgaga tcaacgagag cgccctgaat gcctccttct cttgaggccg
      241 gtccgcggat cgcgcttctc gtcgataaga tcgaatttca tgcaaaggaa ctgacgcgcg
      301 ccttcgccaa gctcggggcg cgcgcggtgc cgatgcggct ctcggccttc agcctcgata
      361 cgaggcggcc gagcgggatt gccgttcccg gcttcggcac gcgtttgccg gacctcgttc
      421 tggtgcgcgc agtcggtctc ggcacattcg aatcaaccac gctgcggctt gggcttttgc
      481 acgccctcgt cggcatgggc gtaccggtcg cgaattcacc gcgatcgatc gaactctgcg
      541 tcgataaggc ggcaacgagc ttccgctgct tcaatcaagg cgttgcgacg ccggaaactt
      601 ttgcgctcga aagcccaagg cagacacgcg cgctggtgcg tcgcgaatcg tcgcgcgggc
      661 cattggtgct caaacctttg ttcggcgcgc agggccgcgg cctcaagctg atccgccacg
      721 aatccgatct gccagacatc gccgaaatgg cgggggtgta ttatctgcag cgctatatcg
      781 gcgtcgaacg cgatggcttc tccgattgcc gcgcgctggt cgcgggcggg cgtgtgcttg
      841 cggcgatgac gcggcgcgcc gatcattgga tcaccaatgt caaacttggc gccaagccgg
      901 agcccgtgac gctggacgac gagatgcgcg atctcgccgt gcgcgccgcc gcggcggtcg
      961 aggcggaatt cgccggcgtc gatctgctgc gtggcgccga tggccgctgg tatgtgatcg
     1021 aggtcaattc catgcccggc tggcaagggc tgcaaagcgt gactgatttt tcgatcgccg
     1081 acgagcttgc cgcactcctg cttgctcgcc acggtctcgc cgccggccca gccgagatgg
     1141 tctcttgatc gctgcggccg agattgcggc cgccttccgc gccgcctgcc gcgaagaaat
     1201 cgaggcgccg aagccgggca atgttcatat ctttgccggc ggccacaata tggaggcggc
     1261 gcatttcctg atgagtgccg atgtatctgc cggtccgatc gccgccggcg cttctgtcgg
     1321 ggcgcgcata ctcgcggcgg ttgaggcaag ctttgccgca gtcggcatga acaccaatct
     1381 tgggattatt ctgctctgcg cgcccttggc agcggcggca gaacgccgcg gcgatttgcg
     1441 tcgcgcgctt gccgatgtgt tggtcggtct cgatcgcgag gatgccgcgc atgtcttccg
     1501 cgccatcgct cttgcgagcc ctggtggcct gggcgatgcc gcgcgttacg acgtgcgaaa
     1561 accggcggat gtcgatctca tggaggccat gcgtgaggcc gcgccgcgcg accggatagc
     1621 ctatcaatat gcgtcgaatt tcgatgatat ttttgtgacg gggtttgcgt cccttgaaga
     1681 cgcgaaagaa gccgggataa atcctcctct tcgagccgtg aatgtgtatc tttctttcct
     1741 cagccgcttc cctgacacgc atatttttcg caaatttggc gcagacacgg ctgccgcagt
     1801 gcagcaggaa gcccgcgaga tattgccgct ttttaaggcg cggccacacg attgtttgac
     1861 tgatcttctg gccttcgacg cgaggctcaa ggcgcgcgga tttaaccccg gaaccagcgc
     1921 ggacctgacc gttgcaactc ttttcgtcga tcggctgcgc aatggcttgc cgatccgccg
     1981 caatgatggt tgattgcgcg ccgtgcggaa ggctccgccg ctatctggcc aaccggcctg
     2041 tcccaaggtg atcggcatac tgcaattgaa tgccgggatc tgacgggcgt cgataacaag
     2101 gataggtcat tggtcccgtc gagcgttcgg cgggatgtct gtgttcagtt ccaccggagg
     2161 aagtacatat ggccaagata accaaacttg gtgttggcga atcgctcgtc ggcgagggca
     2221 acgaggtcgc gcatatcgat ctcatcatcg gaccgcgcgg tagc
//
LOCUS       DASWOG010000106         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_204003,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000106 DASWOG010000000
VERSION     DASWOG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204003"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VGB93_02165"
     CDS             <1..894
                     /locus_tag="VGB93_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0144966.1"
                     /translation="EAPRTGKEATAGRDLASLSARLADLGPRLGSAAGLIAIAVISWW
                     LGGIVFFFVWLVAALVVHFEWQRIVGGERLTTRLALGSVTLIAAAIAVRSQIPALGVF
                     ILLFMALVVGRLGGPGRRAWSGGGLIYAGGLIIAVLTLRSSFPFGRWALGLLFAIVWG
                     TDVAAYFAGRLIGGPKFMPLISPSKTWSGTGVGIITGALIGLGFLAIAARVGQFDAIA
                     PSFALLLLGLILAAVSQLGDLFESWMKRCFGAKDSGHLIPGHGGLMDRVDGFIATAVF
                     AAILGVLRAFPSPAEGLFHWM"
     gene            898..2133
                     /gene="dxr"
                     /locus_tag="VGB93_02170"
     CDS             898..2133
                     /gene="dxr"
                     /locus_tag="VGB93_02170"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0144967.1"
                     /translation="MTLGLELENTRLPRPSSRTPRRIVVLGATGSIGTSTADIIRSSG
                     GAFEVVAVAGGRDAVALAKVARELGARFAALADESGYGALKDALQGSGIEAAAGRKAV
                     IEAALRPADIVMGAIAGTAGVEPTYAALSAGRTVALANKECLVCAGAAFMRQAKAMGT
                     RLLPVDSEHNAIFQALGDAESDTIEMMTLTASGGPFRAWTAEAIARATPEQALKHPNW
                     AMGPKVTIDSAGLMNKGLEVIEAHHIFGINADRLDVLVHAQSVVHGLVAFKDGSVTAG
                     LAAPDMRVPIAHCLGYPERLTTGARRLDLAAVGQLTFERPDFDRFPALRVALDALRTG
                     QGLPTVLNAANEVAVAAFLERRISFHDIARVVEACCEAALRDGTAKEPVSVEDALAVD
                     HIARESAATLLRRGTVSAC"
     gene            2226..3353
                     /gene="rseP"
                     /locus_tag="VGB93_02175"
     CDS             2226..3353
                     /gene="rseP"
                     /locus_tag="VGB93_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0144968.1"
                     /translation="MSIYAHLASVAGTIVPFLFVLSVVVFFHELGHFLIGRLCGVKVD
                     TFSLGFGPELAHFTDRWGTRWRLALLPLGGYVKFHGDANGASMTDDAASVAMPAAERK
                     VTFFGQNVWKRAAIVVAGPIANFILAIVIFTGIFTIHGRGILLPEVEKISHNSAAAAA
                     GFKPNDVILSINGSKVDSFEDVQRVVQASSGRALSFVVLRQGKDITLIATPQQRSGTP
                     GGGLSDVGVLGVEAKAVQANWHIQRYGPLKSFQLANNETWYIVEQTGAYVRGMILGRE
                     STSQLSGPIRIAEVSGEMAKVGFAALLNLAAVLSISIGILNLLPIPLLDGGHLVYYAI
                     EAVRGKALNEKIQQVGFRIGLTFVLGLMILATYNDILRLTK"
     gene            3770..>4708
                     /locus_tag="VGB93_02180"
     CDS             3770..>4708
                     /locus_tag="VGB93_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0144969.1"
                     /translation="MRFIRGQLFRWFLAVVLVAPLLASIPALAAGVEVEGNHRIDAET
                     IASYFPNSDPASVNTGVKALYATGLFSSVDVEHAGGRVIIRVSENNLINRVAFEGNSK
                     VKSDQLTTEIQSKDHGPFNKTIVDADVERIKDLYRRAGEAAASVTYRIVNLPNGRVDV
                     VFKIDEGPKTGVKKIEFVGNHVYSSGKLRDLMQTTEMDWLSFFKSSDVYDPDKIASDL
                     ETIRRFYLKNGYADFHVTGSDAHYDPSQKGYIITITVEEGVQYHVAGVDIESHIPAID
                     GGALHSYLRISPGQIYNGDQAQKTVDALTTQVLRRGY"
BASE COUNT          830 a         1379 c         1474 g         1025 t
ORIGIN      
        1 gaggccccgc gcaccgggaa ggaagcgacg gcggggcggg atttggcttc gctcagcgcg
       61 cggctcgccg atcttggccc acggcttggc tccgccgccg ggctcattgc catcgccgtg
      121 atatcctggt ggcttggcgg aatcgttttt ttctttgttt ggctggtcgc cgcgctggtc
      181 gtgcattttg aatggcaacg tatcgtcggc ggcgagcgac tcacgacgcg acttgctctt
      241 ggttcggtga ctctcatcgc tgcagcgatt gcggtccgct cgcaaattcc cgcgctgggg
      301 gtgttcatcc ttctgttcat ggcattggtt gtcggccggt tgggcggccc ggggcggcgc
      361 gcatggtcag gtggcgggct gatctatgcc ggcggcctga tcatcgccgt cctgacgttg
      421 cgcagctcat ttccgtttgg gcgctgggcg ttaggtttgc tgttcgccat tgtgtggggg
      481 acagatgtcg ccgcctattt cgccgggcgc ctgatcgggg gcccaaagtt tatgccgctg
      541 atttcgccct cgaagacgtg gtctggaacc ggcgtcggca tcatcaccgg tgcgctcatc
      601 ggcctgggct tccttgcgat cgcggcgcgc gtcggtcagt tcgacgcgat cgccccgagc
      661 ttcgctctgt tgcttctcgg gctgatcctc gccgccgtct cgcagcttgg cgatcttttc
      721 gaatcctgga tgaagcgttg tttcggcgcc aaggattccg gccatttgat tccgggtcac
      781 ggcggtctca tggatcgcgt cgatggtttc atcgccacag cagtcttcgc ggccattctt
      841 ggcgtgttgc gggcgtttcc gtcgccagcc gaaggcctgt ttcattggat gtaaagaatg
      901 accttgggac ttgaactcga aaacacgcgc cttccgcgcc cgtcgtctcg gacgccgagg
      961 cgcatcgtcg tgctcggcgc aacagggtcg attgggactt cgaccgccga cattatccgc
     1021 agttctggcg gtgcttttga agtggtcgct gtcgccggcg ggcgagacgc cgtggcactt
     1081 gcaaaggtcg cgcgcgaatt gggcgcccgt tttgccgctt tggcggatga gtccggctac
     1141 ggcgccctga aggacgcgtt gcagggcagc ggcatcgagg ccgcggcggg gcgcaaggct
     1201 gtcatcgagg cggcgctgcg ccctgccgac atcgtcatgg gcgccatcgc cggcaccgcc
     1261 ggggttgagc cgacctatgc agcgctatcg gccggccgga cggtcgctct cgccaacaaa
     1321 gaatgcctcg tgtgcgccgg ggccgccttc atgcgccaag ccaaggcgat gggaacgcga
     1381 cttctgccgg tcgacagcga gcacaacgcg atttttcagg ctttgggcga tgccgaatca
     1441 gacactattg aaatgatgac gctgaccgct tcaggcggtc cgtttcgcgc gtggacggcc
     1501 gaagccatcg ccagggcaac gccggaacag gctttgaaac accccaattg ggcgatgggt
     1561 ccgaaggtga cgatcgattc ggccggcctt atgaataaag ggctggaagt cattgaggca
     1621 catcacattt ttggaattaa tgcggaccgt cttgatgttt tggttcatgc ccagtcggtg
     1681 gtgcatgggc tggtcgcctt caaagatggc tcggtgacgg cgggtctcgc ggcaccggac
     1741 atgcgcgtgc cgattgccca ttgcctgggt tatcccgaac gcctgacgac cggggcgcgc
     1801 cggctcgatc tcgcggcggt cggccaattg acttttgagc gtccggattt cgaccgcttt
     1861 ccagctttgc gcgtggcgct tgacgccttg cgcaccgggc agggactgcc gacggtactc
     1921 aatgccgcaa atgaagtggc ggtcgcggcg ttcctcgagc ggcggatttc cttccacgat
     1981 attgcccgcg tcgtcgaggc ctgctgcgag gcggcgctgc gcgacgggac ggcgaaagag
     2041 cctgtatccg ttgaggacgc gttggccgtt gaccatattg caagagaaag cgccgctacc
     2101 ctcttgcgga ggggcacggt ttcggcatgt taacaactcg ctaaccaaat cggttagcca
     2161 tggagttcgt gctgattgcg tctttggccg gtttctcagg ccagcgtggc gcaaaaagga
     2221 gtaaaatgtc gatttatgct catcttgctt ccgtcgccgg cacgatcgtg ccctttcttt
     2281 tcgttctgag cgtcgtcgtc ttcttccacg agctcgggca tttcctcatc ggccgcctct
     2341 gcggcgtgaa ggtggatacg ttctctctgg gattcgggcc cgagctggcg catttcaccg
     2401 accgctgggg cacgcggtgg cgcctcgcac tcctgccact tggtggctat gtaaagtttc
     2461 atggcgacgc caacggtgcg tcgatgacgg acgatgctgc gagtgtggcc atgcccgccg
     2521 ccgagagaaa ggtgaccttc ttcggccaga acgtctggaa gcgggctgcg atcgtcgtcg
     2581 ccgggccaat tgccaatttc attctcgcaa tcgtgatctt cactggaatt ttcaccatcc
     2641 acggccgcgg catcctgctg ccggaggtcg aaaagatcag tcacaacagt gcggccgcag
     2701 ctgccggttt taagcccaac gacgtcatcc tctcgatcaa cggcagcaaa gtcgatagct
     2761 ttgaggatgt gcagcgggtg gtacaggctt caagcggcag ggcgttgagc tttgtcgtgt
     2821 tgcgtcaagg caaggacatc acgctcattg cgacacccca acagcgcagc ggcactcccg
     2881 gcgggggcct gtcggatgtt ggcgtgcttg gcgtcgaggc caaggctgtc caagccaatt
     2941 ggcacatcca gcgctacggt cccctgaaat cattccagct tgccaataac gagacctggt
     3001 acatcgtcga acagacaggc gcctatgtgc gcggcatgat tctcggccgc gaatcgacct
     3061 cgcaattgtc cgggccgatc cgaatcgcgg aggtttcggg cgagatggcc aaggtgggat
     3121 tcgcagctct ccttaatctt gccgcggttt tgtcaatttc gattggaatt ctcaacctgt
     3181 tgccgatccc actgctcgat ggcggccatc tggtttacta tgcaatcgaa gctgtgcggg
     3241 ggaaggcgct gaacgaaaag atccagcaag tcggtttccg aatcggactg accttcgtgc
     3301 tgggccttat gattttggcg acgtacaacg atatcttgcg gctgacgaag tgattccaaa
     3361 gtcgcgccgc acacagctcg gtgcgcggcc aaacgggggc gttccggcca tgctcggccg
     3421 gtaaatggat acgtgcgcca aaatgcgcaa ttaaccatcg cccttaacgc gacgctcacg
     3481 ggattaagcg tgtcttactc gtcacgcggt tccgaaaacg acgaagctct ggcaaacgcc
     3541 atttgcagag taaatcaaac cctgtagaag cggagcctgg gttggcggga ctggcggcga
     3601 aagcgccgcc agcgcggcgg gttcggcctg aggccgaatc cgggactgac ccttggcgag
     3661 ccggaggtcg tccgaagaac tgacttgggg tggggtgttg cgggtttctg ggctcaagaa
     3721 gccgattccg cagcagaagt taggtcgata acgcggggac cgggcgtaga tgcgatttat
     3781 tcggggtcaa ctgttccggt ggtttctcgc cgttgttctt gtggcgccgc ttctggcgtc
     3841 gattcctgct cttgccgctg gcgttgaggt tgagggcaac catcgcatcg acgccgaaac
     3901 gatcgcatcc tattttccga attcggaccc ggcgtcggtc aataccggcg tcaaggcgct
     3961 ttatgcgacc ggcctgttct ccagcgttga cgtggagcac gcgggcggcc gcgtcatcat
     4021 ccgcgtctct gagaataacc tcatcaatcg cgtcgccttc gagggcaaca gcaaggtcaa
     4081 atccgaccag cttacgaccg aaatccaatc caaggatcac ggcccgttca acaagaccat
     4141 cgttgacgcc gacgtcgagc gcatcaagga cctttaccgt cgcgccggcg aagccgccgc
     4201 gagcgtcact taccgaatcg tcaacctgcc gaacggtcgc gtcgatgtcg tcttcaagat
     4261 cgacgaaggc ccgaaaactg gcgtgaagaa gatcgaattt gtcggcaatc acgtctattc
     4321 atcgggcaag ttgcgcgacc tgatgcagac gacggaaatg gactggctgt cgttcttcaa
     4381 gtcatccgac gtgtacgatc cggacaagat cgcctcagac cttgagacca tccgccgctt
     4441 ttatctgaag aacggctatg ccgacttcca cgtcaccggc tcggatgccc actacgatcc
     4501 gtcgcagaag ggctacatca tcacgattac cgtggaagaa ggcgtgcaat accacgtcgc
     4561 gggtgtcgat atcgaatccc atatcccggc gatcgacggt ggcgcactgc attcctatct
     4621 gcgcatctcg cccggccaga tttataacgg cgaccaggcg caaaagaccg tcgacgcctt
     4681 gacgactcag gttttgcgtc gtggctat
//
LOCUS       DASWOG010000107         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_94271,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000107 DASWOG010000000
VERSION     DASWOG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94271"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGB93_02185"
     CDS             <1..690
                     /locus_tag="VGB93_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY0144970.1"
                     /translation="PAYAPPPPPPPSWTGLYVGVSAGYGGDQVHYNHYSAGVLDNTGS
                     LSSSGFLGGGTIGFNYQFPSTNYVLGVEGDFDGSTVRANDDSPFGVGGGSIPNDTKLD
                     WFATARARFGYAFGNILPYVTGGGAFGHVDQLRVDPGANPFGNFSVGSTRTGWTAGAG
                     IEYQVTHNLSVKAEYLHVDLGRQYYQTPGEIAFAPVNDHSAHPTLNVVRAGVNWKFEW
                     LTPPTPVVPKY"
     gene            914..1633
                     /locus_tag="VGB93_02190"
     CDS             914..1633
                     /locus_tag="VGB93_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY0144971.1"
                     /translation="MVRSSFLALIAILAPLAGVRAADLPSEKGPALYAPPPLVFSWTG
                     FYLGATAGGAFNRQTLSAGPFGSLGASPAGFVGGGEAGFNYQFPTSNFVLGGETDFQG
                     STLRADYGVPGVGLNGSSRLDWWGSARARLGLSFGNILPFVTGGFAYGDVHDQIGLGG
                     ASFSNGGVRTGWTAGAGVEYAITRNLTAKAEYLYTDLGSVSTPLGGGTARTRADFHAV
                     RAGLNWKFDWSGAPEPVTSKY"
     gene            1893..3161
                     /gene="rho"
                     /locus_tag="VGB93_02195"
     CDS             1893..3161
                     /gene="rho"
                     /locus_tag="VGB93_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0144972.1"
                     /translation="MRDIKLQDLKSKSPTELLAFAEEHEVENASILRKQELMFAILKQ
                     LASRDIEIVGEGVIEVLQDGFGFLRSPDANYLAGPDDIYVSPSQIRRFGLRTGDTVEG
                     LIRSPKEGERYFALLKVNTINFEDPEKIRHKVHFDNLTPLYPDERLKLEIEDPTKKDF
                     SARVIDIVAPIGKGQRALIVAPPRTGKTVLLQNIAQSITTNHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNSEIILDRKVADKRSYPAIDIARSGTRKEELLVPADILKKMYVLRRILNP
                     MGTVDAIEFLLGKLRETPKGNASFFESMNT"
     gene            complement(3202..3615)
                     /locus_tag="VGB93_02200"
     CDS             complement(3202..3615)
                     /locus_tag="VGB93_02200"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0144973.1"
                     /translation="MTHSEMALTQSETPPAEAPVIRTIAMPADANPYGDIFGGWLMSQ
                     MDLAASNVASRVARGRASTVRVEAMNFLRPVRVGDVVSLYGRLIGRGRSSMKIEVEAW
                     RRARDGEDEEKVTEAVFTFVAIDPVTRKSRPLPEE"
     gene            complement(3684..>4022)
                     /locus_tag="VGB93_02205"
     CDS             complement(3684..>4022)
                     /locus_tag="VGB93_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0144974.1"
                     /translation="TRADALFAAVAAATNANPIGHTGILNALDLYLRGREIVTAGPER
                     APLYHAALAAPFDTRMVLDLETVNGLPPDHPAQAQIAASGTAAAFVCTGGVCSLPVHD
                     NAGLDALLAR"
BASE COUNT          758 a         1225 c         1226 g          813 t
ORIGIN      
        1 ccggcctatg cgccgcctcc acctccacca ccgtcttgga cggggctcta tgtcggcgtc
       61 agcgctggct acggcggcga tcaggtccat tacaaccatt acagcgccgg cgttctcgac
      121 aatacgggaa gcctgtcttc cagcggcttt ctgggcggcg ggacgatcgg cttcaattat
      181 cagttcccat cgacaaatta tgtgctgggt gtcgaaggtg atttcgacgg ctcgaccgtc
      241 cgtgcgaacg acgacagccc gtttggcgtc ggcggcggct cgatcccgaa tgataccaag
      301 ctagactggt ttgcgacggc gcgggcgcgg ttcggttatg ctttcggcaa catcctgccc
      361 tatgtgacag gcgggggcgc ctttgggcat gtcgatcaat tacgcgtcga tcccggcgcc
      421 aatcccttcg gcaatttctc cgtcggttcg acgcggacgg gctggaccgc gggtgcgggc
      481 atcgaatatc aggtcaccca caatctctcg gtgaaggcgg aatatctgca cgttgatctc
      541 ggccggcaat attaccagac gccaggcgag atcgcgttcg cgccagtgaa cgaccattcg
      601 gcgcatccga ccctgaatgt cgtgcgcgct ggcgtgaact ggaagtttga atggctgacc
      661 ccgccgacgc cggtcgtgcc gaaatactga tcgaaaaagt tcgagacgga tggggaccgg
      721 aggtgacatc cgccggtttt ttgttgcgcc gatatcacag tgctgcgggc attggtcgtc
      781 cagggcattt ttgctggaac gaggtgcgtt ttcgcacatt cgccccgtga gccttggcct
      841 ttaaccctgt gaacgccatt taatgtgcga ggcgggcagg tttcggctag gttttccaaa
      901 acggcggtca ctgatggttc ggtcgagttt tctggcactc atcgcgatct tggcaccgct
      961 cgccggtgtg cgggcggctg acctgccgag cgagaagggg cctgccctct acgcgccgcc
     1021 gccgctggtt ttttcctgga ccgggttcta tcttggcgcg accgcgggtg gcgccttcaa
     1081 tcgtcagacg ctgagcgccg ggccgttcgg cagcctgggc gccagccccg ccggtttcgt
     1141 cggcggtggc gaggcggggt tcaactacca gttcccgacc tcaaatttcg tcctcggcgg
     1201 cgagaccgat tttcagggtt cgaccctgcg cgccgattat ggcgtccccg gtgtgggcct
     1261 gaatggttcg agccgtctcg actggtgggg gtcggcgcgg gcgcgtctcg gtctttcgtt
     1321 cggcaacatt ctgccgttcg tcacgggcgg ttttgcctat ggcgacgtgc atgatcagat
     1381 cgggcttggc ggcgccagct tttcgaatgg gggtgtgcgc acgggatgga cggcgggcgc
     1441 cggcgttgaa tatgcgatca cccgcaatct gacggctaag gcggaatatc tctataccga
     1501 cctcgggtct gtttcgaccc cgcttggcgg cgggacggcg cggacgcggg cggacttcca
     1561 cgctgtacga gctgggttga actggaagtt cgactggagt ggcgcgccgg agccggttac
     1621 gtccaaatat tgatccgctt ggggtgcaaa ccgtcaaact ggcatctaat tcgctccata
     1681 attgtcgtgt gatgtttgac agcgcgccga tgcccagttg tcaaagaggc cgaaacgtcc
     1741 tatttttgtt atctctcccc gcaggcgatt tgtcggcgcg caccgtttaa gcgcccctcg
     1801 atacgacatt ggcctgcctt cccaggatct ctgacgccca ttcgggcccc ctaagctgcg
     1861 cgcccagcgc ccttccaagg acgaaaatcc cgatgcggga tatcaaactt caagatttaa
     1921 aatccaaatc gccgaccgaa ctcctcgctt tcgccgagga acacgaagtc gaaaacgcct
     1981 cgatcctgcg caagcaggaa ttgatgttcg ccatcctcaa acaactcgcc agccgcgata
     2041 ttgaaatcgt cggcgaaggc gtcatcgaag tgctgcaaga cggcttcggc tttctgcgct
     2101 cgccagacgc gaattatctc gccggccccg acgacatcta cgtgtcgccg tcgcagattc
     2161 gccgtttcgg ccttcggacc ggcgatacgg tcgaaggcct gatccgcagc ccgaaagagg
     2221 gcgagcgtta tttcgcgctg ctcaaggtca acacgatcaa tttcgaagac ccggagaaga
     2281 tccgtcacaa ggtccatttc gacaatctga cgccgctcta tcccgatgag cggttgaagc
     2341 ttgagatcga agacccgacc aagaaggact tctccgcccg cgtcatcgat atcgtcgcgc
     2401 cgatcggcaa gggccagcgc gctttgatcg tcgcgccacc gcgcaccggc aagaccgtgc
     2461 tgttgcagaa catcgcccag tcaatcacca ccaatcatcc ggaatgctat ctgatcgtcc
     2521 tgctgattga cgagcggccg gaagaagtga ccgacatgca gcgctcggtg aaaggcgagg
     2581 tcgtctcctc gaccttcgac gaaccggcgg tgcgccacgt tcaggtcgcc gaaatggtga
     2641 tcgaaaaggc taagcgcctg gtcgagcacg gccgcgacgt cgtgatcctg ctcgactcga
     2701 tcacgcgtct tggccgcgcc tacaacaccg tcgtgccgtc atccggcaaa gtgttgaccg
     2761 gcggtgtcga cgccaatgcc ttgcagcggc cgaagcgctt cttcggcgcc gcgcgcaata
     2821 tcgaagaagg cggctcgctg acgatcatag cgacggcgct gatcgacacc ggctcgcgta
     2881 tggatgaagt gatctttgaa gaattcaaag gcaccggcaa ttcggaaatc atcctcgacc
     2941 gcaaggttgc ggacaaacgc tcctatccgg cgatcgacat cgcccgctcc ggcacccgca
     3001 aggaagagct gctcgtcccg gcggacattc tcaagaagat gtatgtcctt cgccgcatcc
     3061 tcaatccgat ggggacggtc gatgcgatcg aattcctgct gggcaaattg cgcgagacgc
     3121 cgaagggcaa tgcgagcttc ttcgaatcga tgaatacgta aggctgcgtc attgcgagat
     3181 gcttgcatct cgcaatgacg gctattcctc cggcaacgga cgcgatttgc gcgtcactgg
     3241 gtcgattgcg acaaaggtga agaccgcctc ggtgaccttc tcctcgtcct cgccgtcacg
     3301 ggcgcggcgc cacgcctcaa cctcgatctt catcgaacta cgtccgcgcc cgataagccg
     3361 gccatagaga ctgacgacat cgccgacgcg gacgggtcgc agaaagttca tcgcctcgac
     3421 gcgcaccgtc gaagcacggc cgcgtgcaac gcgcgaggcg acattgctgg cggcaagatc
     3481 catctgcgac atcaaccagc cgccgaaaat atcgccgtaa gggttggcgt ccgccggcat
     3541 ggcgatggtg cggatcactg gcgcttccgc cggcggcgtt tcactctgcg tcaaagccat
     3601 ttcgctgtgg gtcatgggca tccctcatga ttttcgcatc ggcttgccca aaccgcaggg
     3661 cactttttgg gccgatgctc atgtcaacgc gcgagcaaag catcaagccc ggcattatca
     3721 tgaaccggca atgagcacac gccgccggtg caaacgaatg cagcggccgt gccggacgcg
     3781 gcgatctgtg cctgcgccgg atgatccggt ggcaggccgt tcactgtttc gagatcgagc
     3841 accatgcgtg tatcgaaagg cgcggcgagc gctgcatgat aaagcggcgc gcgctcgggc
     3901 ccggcagtga cgatttcgcg gccgcgtaga taaagatcga gcgcgttcaa aatgccggta
     3961 tgaccgatcg gattggcgtt tgtggcagcc gcgacggcgg caaagagcgc gtctgcccgc
     4021 gt
//
LOCUS       DASWOG010000108         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_57450,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000108 DASWOG010000000
VERSION     DASWOG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57450"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..664)
                     /locus_tag="VGB93_02210"
     CDS             complement(221..664)
                     /locus_tag="VGB93_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144975.1"
                     /translation="MKFSAKAALAITLALCPVLAIATDHTISAMTYSGKKVRLDVENM
                     NGHMMALLPAEQAPDYLHQMIFEVMNASGKHFRLVGTNVNGHMMLMVPDDQMPDFLHQ
                     MTFTVMTHSGKEMTVAAADINGHMMVMIPADQATDYLHQMILRVQ"
     gene            905..>1584
                     /locus_tag="VGB93_02215"
     CDS             905..>1584
                     /locus_tag="VGB93_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0144976.1"
                     /translation="MTLEQLRIFVAVAERQHVTRAAEALNLTQSAASAAVAALEAQHR
                     SKLFNRVGRGIELTDAGRIFLDEARAVLARAELAELKLAELAGLKRGRLLIQASQTIA
                     SYWLPRHLVDFRHAHPGIEIRLSVGNTADAAEAVSTGAAELGFVEGPVERPLLKVTEV
                     ARDQLIIVVAAGHPWATRKKLTPDDLVKTEWVLREPGSGTRSVFEAELAKFGVPVTAL
                     NVALELPT"
BASE COUNT          302 a          441 c          480 g          361 t
ORIGIN      
        1 atgtcgagac tgatgtcctg gcgtgcgatg ggtgagtcga gatgatgtga catggctggt
       61 ctcgcgttag atggagctgg aattctctta gctttgccga cagcgattat gtgggcctcg
      121 cgcgaaccat ggcccgtttc catgtttgcc gcgttcttct tgcgcggctg tcttcttgct
      181 cttcgctgtt tataggctgc gccgaccggg tgagccagtg ttattgcacg cgtaagatca
      241 tctggtgtag gtaatcggtc gcctggtccg ccgggatcat aaccatcata tggccgttga
      301 tatcggcggc cgcgacggtc atttccttac cgctatgtgt cataactgtg aatgtcattt
      361 ggtgcaggaa gtcaggcatc tggtcgtcgg gcaccatcag catcatgtgg ccgttcacgt
      421 tcgttccgac gagccggaag tgcttgccgc tcgcattcat gacctcgaaa atcatttgat
      481 ggagataatc cggcgcctgc tcggctggca gcagcgccat catgtgaccg ttcatgttct
      541 cgacgtcgag acgcaccttt ttgccgctgt acgtcatggc ggatatcgta tgatccgtcg
      601 caatcgccag gacgggacag agagcgagcg taatcgccag tgcggccttc gcggaaaatt
      661 tcatcgtcag tttccctcta taatcgagcg ttcccggtcg gaaacttaag cattgcgatt
      721 cagaaagcgg ccgacgaagc cggcagggta ctcgtgctgt tcggaccgcc aatttggccg
      781 ccagaccata aggagcggct gcgctctcat ctttcaaaat ccaacggccc atttcataat
      841 cgaacgacgt aaggaagatg accgtccggt gacttgcgcg cgcggacgta ttaaagacgg
      901 gttcatgact cttgagcagc ttcgtatttt tgtcgccgtt gcagagcgtc aacatgtgac
      961 gcgggctgcc gaggcgctta atcttacgca atctgcggcg agcgccgcgg tcgccgcgtt
     1021 ggaagcgcag catcgctcga aacttttcaa tcgtgtcggc cgggggatag aactgaccga
     1081 cgcagggcgg attttcctgg atgaggcccg tgctgtgctg gcccgcgctg aattggcgga
     1141 actaaagctc gccgaacttg cgggcctgaa gcgcggacgg ttgctcatcc aggcgagcca
     1201 gacgatcgcg agctattggc tgccgcgtca tctcgtcgat ttccgtcacg cgcatccggg
     1261 catcgaaatt aggttgagtg taggcaacac cgccgatgcg gcggaagccg tcagcacggg
     1321 cgcggccgaa ctcgggttcg tagaagggcc ggtcgaacgg ccgttgctca aggtcacgga
     1381 agtagcgcgt gaccaattga tcatcgttgt cgcggccggt catccctggg cgacgcgcaa
     1441 gaagctcacg cccgacgatc tcgttaaaac cgaatgggtt ctgcgcgagc cgggctccgg
     1501 cacgcggtcc gtctttgaag ccgagcttgc caaattcggc gttccggtca cggccctcaa
     1561 tgtcgcgctc gaattaccga ccaa
//
LOCUS       DASWOG010000109         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_112600,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000109 DASWOG010000000
VERSION     DASWOG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112600"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..167
                     /locus_tag="VGB93_02220"
     tRNA            79..167
                     /locus_tag="VGB93_02220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Leu,seq:taa)
     gene            complement(218..853)
                     /locus_tag="VGB93_02225"
     CDS             complement(218..853)
                     /locus_tag="VGB93_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEY0144977.1"
                     /translation="MKTNDFIGLLSADSAPVWRFGSIFGLAIVFGILIAGSAFFLGIG
                     VRPDFSQAIQSPRFLFKFVATGMLAAGATGTMLSLARPCASLKRWSWILMAVPLLLAC
                     AAAFELIVMPRDVWMKLLVGKNSRCCLTLIPLLAIGPLACLLIALRQGASDKPGVAGG
                     LAGMAASGIAATFYAANCTDDSPLFVATWYPLAIIAISAVGCVAGVKFLKW"
     gene            1070..1375
                     /locus_tag="VGB93_02230"
     CDS             1070..1375
                     /locus_tag="VGB93_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY0144978.1"
                     /translation="MSVKTIVSAAILAGAVTTALSTIAAAAPLTKAEVHAALAAHKEK
                     CFGVALKGQNDCAAGPGTTCQGTSTVDFQTNSWKFVQGGTCTGIVLPNGSHGMLKAM"
     gene            1430..>1831
                     /locus_tag="VGB93_02235"
     CDS             1430..>1831
                     /locus_tag="VGB93_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEY0144979.1"
                     /translation="MIEPAVPHAAIPLRFPAHPIAGLAGTSFKHEHLPAILAQENQGG
                     FFEVHAENYMGAGGPPHHAFERVRRDYPISLHGVCMSIGGPQPLDKAHIETFRSLVER
                     YDPVLVSDHLAWSTHEPTFFYDLMPLPYTEAT"
BASE COUNT          410 a          548 c          527 g          346 t
ORIGIN      
        1 gcgccgatcg ccggcggcaa gggatagttc aagcgataga tttgcgtcga gcgccgcggc
       61 gctttatggc gaggttacgc gggcgtggcg aaattggtag acgcaaagga cttaaaatcc
      121 ttcggctcga aagagctgtg ccggttcgac cccggccgcc cgcaccatcg tcgcaaacct
      181 aagtcgctac gtgccaagct ccgtcgctga agccgcacta ccatttgagg aatttcacgc
      241 cggcaacaca tcccacggcg gatatcgcga tgatggcgag cggataccag gtggcgacga
      301 aaagcgggct gtcgtcggtg caattcgctg catagaacgt cgccgcaata ccgctcgcgg
      361 ccatgccggc caatccacca gcaacgcccg gcttatcgga tgcgccttgc cgcaacgcaa
      421 tgagcaagca ggcgagaggg ccgattgcga gaagcggaat cagagtcagg cagcagcgcg
      481 aatttttgcc gaccagcagt ttcatccaca catcgcgcgg catcacaatg agttcgaagg
      541 cggccgcgca cgccagcaac aagggcacgg ccatcaatat ccacgaccag cgtttgagcg
      601 aggcgcaggg gcgcgcgagc gaaagcattg ttcccgtcgc accggcagcc agcattcctg
      661 tcgcgacaaa tttaaaaagg aagcgcgggg attgaatggc ttgggaaaaa tccggcctga
      721 ccccgattcc tagaaagaat gcgctgccgg cgatgagaat tccaaataca attgcgaggc
      781 cgaagattga tccaaatcgc caaacaggcg cagagtcggc cgacagcagg ccgatgaagt
      841 cgttggtctt catgatggct gctcccgata tccgctttga cgccaatggt ttttatctga
      901 accttttacg acgctggccc cgtcttcgtt acaggtggcc cagtctattc gatcccgcgc
      961 cacatcacgg aactgtgaga cctgcgatcc gtaacgggtg ctgacggcca gccgtaggca
     1021 ccaatgcacc ccgtaaacgg atgcatgaag cctcaacctg gagaatctca tgtctgtgaa
     1081 aaccatcgtg agcgcggcta ttctcgccgg cgctgtgacg accgcgctgt cgacgatcgc
     1141 cgctgccgct cctttgacga aggcagaggt gcatgctgca ctcgccgcgc acaaggaaaa
     1201 gtgcttcggc gttgccctca agggccagaa cgattgcgcg gccggcccgg gcacgacctg
     1261 tcagggcacg tcgaccgtag acttccagac caattcctgg aaattcgtac agggcggcac
     1321 ctgcaccggc atcgtcctgc ccaacggcag ccatggcatg ctgaaggcga tgtaacgtca
     1381 tctcataggg accgacaaac gccggaaagc ggagaaaaac gtgaattcaa tgatcgagcc
     1441 cgctgtcccc cacgcggcga tccctctgcg ctttccggcg cacccgattg cgggcctggc
     1501 gggaacaagc ttcaaacatg agcacctgcc ggcgatcctc gctcaagaaa accagggcgg
     1561 ttttttcgag gttcacgccg aaaattatat gggtgccggt gggccgccgc atcacgcgtt
     1621 tgaacgtgtc cgccgcgact atccgatatc tttgcatggc gtctgcatgt cgatcggtgg
     1681 gccgcagccg ctcgataaag ctcatattga aacgttccgg tctctggttg aacgttatga
     1741 tccggtactc gtttcagacc atcttgcttg gtcgacgcat gagccgacct ttttctatga
     1801 tcttatgccg ttgccttaca ccgaagcaac a
//
LOCUS       DASWOG010000110         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_350778,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000110 DASWOG010000000
VERSION     DASWOG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350778"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGB93_02240"
     CDS             <1..577
                     /locus_tag="VGB93_02240"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY0144980.1"
                     /translation="IYGPQTGLVLAAAGTTQIFILDRRIGAFVRTHDGINIPRKSKEY
                     AINASNYRHWAPSVRTFVDDCIKGTEGPLGQEQNMRWIASLVAEAYRILARGGVFLYP
                     GDMRQGYSAGRLRLIYEANPISLIIENAGGTATDCARRILDIQPTDIHARTPFVFGSD
                     EVVRLITRYHTDPQFSAEHSPLFGRRGLMRF"
     gene            588..1463
                     /locus_tag="VGB93_02245"
     CDS             588..1463
                     /locus_tag="VGB93_02245"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY0144981.1"
                     /translation="MSAKHPIISVTGSSGAGTTSVRRTFEHIFRREEIGAAFIEGDAF
                     HRFDRDEMRRVMNEEEQRGNNHFSHFGPTANLLEELESVFRTYAETGQAKTRHYVHDA
                     IEADLYRGTPGQFTDWGPLPAQSEVLFYEGLHGAIVTDKVNVARYADLKIGVVPVINL
                     EWVQKIHRDKAARGYSKEAVTDTILRRMPDYVNYICPQFTETDINFQRVPTVDTSNPF
                     AARWIPTSDESMVIIRFKDPRGIDFSYLLSMLHDSFMSRANSIVVGGGKLDLAMQLIL
                     TPIILQLVERARRAL"
     gene            1475..>2143
                     /locus_tag="VGB93_02250"
     CDS             1475..>2143
                     /locus_tag="VGB93_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0144982.1"
                     /translation="MNVFAKAEAAVTHDELANAIRFLAIDAVEKANSGHPGMPMGMAD
                     VATILFTQFLKFDPADPAWHDRDRFVLSAGHGSMLLYALLYLTGYPEMTIEEIKNFRQ
                     LGSKTPGHPEYGHTPGVETTTGPLGQGLAMAVGMAIAEKLLAARYGNDLVDHRTYVIA
                     GDGCLMEGVSQEAITLAGHLKLSKLIVLWDDNKITIDGAVSLADSTNQIARFKAAGWD
                     AWHID"
BASE COUNT          426 a          665 c          620 g          432 t
ORIGIN      
        1 catttacggg ccgcagaccg gactcgttct cgcggcagcg ggaacgacgc agattttcat
       61 tctcgaccgg cgcattggcg ccttcgtgcg gacgcacgac ggcatcaata ttccgcgcaa
      121 gtcgaaggaa tatgcgatca acgcctcgaa ctaccggcac tgggctccgt cggtccgcac
      181 gttcgtcgac gattgcatca aaggcacaga aggccccctc gggcaggaac agaacatgcg
      241 ttggatcgcg tcgctcgtcg cggaagccta tcgcatcctg gcacgcggcg gcgtgttcct
      301 ttatcccggc gatatgcgcc aaggttatag cgcgggccgg ctgcggctta tctatgaggc
      361 aaatccgatt agcttaatca tcgaaaacgc aggcggtacg gccaccgatt gcgcccgccg
      421 cattctcgac attcagccga ccgacattca cgcgcgcaca cctttcgtct tcggctcgga
      481 tgaggtggtg cgtctgatca cgcgctatca taccgatccg caattctcgg cggaacattc
      541 gccgcttttc ggccgccgcg ggctgatgcg gttctaacgg ggacgcgatg tcggcaaaac
      601 atcccatcat ctctgttacg ggttcttccg gcgccggcac gacttcggtg cggcggacct
      661 tcgagcacat cttccggcgc gaggagatcg gcgccgcctt catcgagggc gatgcgtttc
      721 accgcttcga ccgcgacgag atgcgtcgcg tcatgaacga ggaagagcaa cgcggcaata
      781 atcatttcag ccatttcggg cccacggcca atctgctcga agaactcgaa tcggtgttcc
      841 gcacttatgc cgagacgggt caggccaaga cgcgccatta cgttcacgat gcgatagaag
      901 cggacctcta tcgcggaaca ccgggccagt tcacggattg ggggccgctg cccgcgcaat
      961 ccgaggtgct tttctatgaa gggctgcacg gcgcgatcgt caccgacaag gtgaatgtcg
     1021 cgcgttatgc cgatctcaag atcggcgtcg tgccggtcat caatctcgaa tgggttcaaa
     1081 aaatccatcg cgacaaggcc gcgcgcggct attccaagga ggccgtcacc gacacgattt
     1141 tgcggcggat gccggactat gtgaattaca tctgtccgca attcaccgag accgacatca
     1201 acttccagcg tgtgccgacg gtcgatacat cgaacccctt tgcggcgcgc tggattccga
     1261 cctcggatga atcgatggtc atcatccgct tcaaggaccc ccgcggcatc gacttctcgt
     1321 atctgttgtc gatgctccac gatagcttta tgtcgcgggc caattcgatc gtcgttggcg
     1381 gcggcaaact cgaccttgcc atgcagctca tcctgacgcc gatcattctg caattggtcg
     1441 aacgcgcgcg gcgcgcgctg tgaaggagag ttcaatgaac gtgttcgcca aggctgaagc
     1501 tgccgtcaca cacgacgagc ttgccaatgc gatccgcttt ctcgccatcg acgcggtgga
     1561 aaaagccaat tccggccatc ccggcatgcc gatgggcatg gccgatgttg cgacgatcct
     1621 tttcacgcaa tttttgaaat tcgatccggc cgatcccgcg tggcacgacc gcgaccgctt
     1681 cgtgctttcg gccggccacg gctcgatgct gctctatgcg cttctctatc tcaccggcta
     1741 tccggagatg acgatcgagg agatcaagaa tttccgtcaa ctcggttcaa aaacgccggg
     1801 ccatcccgaa tatggccata cgccgggcgt cgagacgacg accggcccgc tcggccaggg
     1861 ccttgccatg gcggtcggca tggcgatcgc tgagaaactg ctcgccgcgc gctatggcaa
     1921 cgatctcgtc gatcatcgca cctatgttat cgcgggcgat ggctgcctga tggagggcgt
     1981 cagccaggag gcgatcacgc tcgccggcca tctgaagctc tcgaaactca tcgtgctctg
     2041 ggatgacaac aagatcacca tcgacggcgc ggtgagtctc gccgattcga cgaatcagat
     2101 cgcgcgtttc aaggccgcag gttgggacgc atggcacatc gac
//
LOCUS       DASWOG010000111         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_314352,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000111 DASWOG010000000
VERSION     DASWOG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314352"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..2088
                     /locus_tag="VGB93_02255"
     CDS             94..2088
                     /locus_tag="VGB93_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144983.1"
                     /translation="MKARAASSFVVANETPARVHAETHQSIFATALHDGGVVLCLIAI
                     ALWTLTHPFQGIAGDTNVYIGRALADLNPTGVGRDMMFVDDGQSRFSLFPFLLDHLVA
                     ALGTQTTVMMLAILAMVAWTSALAFFARHYVGWRFVPVVLIFVAVLPTFYGSIWHFGY
                     SEVQSVPRPFAEACVLGALAALASRRALLSVALLVPATLLHPIMALAGWVVLAIVLCV
                     KDRRWLIAAGLTVAAAIIAGVLGLPLFDRLVRLMDPDLKALAQSRSGLLFPTHWSIAS
                     YAPIVLQSATIASAARFFEGRRKLILLAVVLAGIGGIAAQSLFGDELSLELVIQAQFW
                     RMTWLLAAIGAVSMAICAIKLIQNGRVGYLVLALLTAAWLCYLDFMMVSALCAAALVV
                     QFSAPRLASVVTRRVVIVVWICVAAYGLLLNVLYFEQFGRFFASLPAEGPGGFTYFWN
                     NWRYIAFPIAGGALALALAPRARVTWAIEGFAALALAAVAWHLWDDRSAFQKIVDRNR
                     RPAPLEQLLAGKDGEILWLGGQTEAWFLTGRPQWASPQQGVGTIFSPKLAREWRGHMQ
                     FLIDNGLVEKEGLAISHNLSSAELPVVTAPAVGRLCARADAPAWIVAPVYEGATLPAA
                     LKAQFWTPPVPNFRMTEEPDGYVWHRISTYAVLPCVGAQL"
     gene            2132..>3472
                     /locus_tag="VGB93_02260"
     CDS             2132..>3472
                     /locus_tag="VGB93_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144984.1"
                     /translation="MNAISVAFTPLLPPALLWGFAALALLVTVLSLLRRRRGALLRAL
                     GLGLVLLALTDPSLVRENRKPLKDVVAIVVDQSGSQTIGDRMAQTQKATADLKRWLEA
                     LGNIDVRIIESGRNDTENNGTRLFSALNEGLADVPAERIGAVFMITDGVVHDIPSSTA
                     ALGFKAPLHAFITGHEGERDRRIELLTAPRFGIVGKDVTLELRVDDTTNGTEPVVLHV
                     RRDGNSIADVTAAIGETISVPVKIEHGGPNVVELEVEALPGELTALNNKAVLTIDGVR
                     DKLKVLLVSGEPNQGERMWRNLLKSDANVDLVHFTILRPPEKQDGTPINELALIAFPT
                     ADLFGRKITDFDLIIFDRYSNQAILPAVYLDNIVRYVHDGGALMIAAGPDFASSDGIY
                     YSPLGALSPAEPTGDVIQQAFQPAITAVGARHPVTRGLPGADETPPAWSRWFRQV"
BASE COUNT          550 a         1086 c         1085 g          751 t
ORIGIN      
        1 cctcgccaag gcgccgaccg caggtttcgt cttcacgttc aacttcttcg cccggcgccc
       61 gctgctgttc gtgtcggagg cgactacctc gaaatgaagg cgcgcgcggc ctcttcgttc
      121 gttgtcgcaa atgagacgcc ggcgcgggtc cacgctgaaa cacatcaatc gatatttgcc
      181 acggcgctgc atgacggcgg tgtcgtactt tgcctcattg ccatcgcgct ttggacgctg
      241 acgcacccgt ttcaaggcat cgctggcgac acaaacgtct acatcggccg ggcgctcgcc
      301 gacctcaatc cgacgggcgt cggccgtgac atgatgttcg tcgatgacgg acaatcgcgt
      361 ttcagcctgt ttccgtttct gcttgaccat ctcgtcgcgg cgttgggaac acagacgacg
      421 gtgatgatgc tcgcgatcct cgcgatggtt gcgtggacgt cggcgctcgc gttcttcgcg
      481 cgccattatg tcggctggcg atttgttccc gtcgttctga ttttcgtcgc tgtgctcccg
      541 accttctacg ggtcgatctg gcatttcggt tattcggaag ttcagtccgt gccccggccc
      601 ttcgcggaag cgtgcgtact gggcgctttg gcagcgctgg catcacgccg cgcgcttttg
      661 agcgttgcct tgcttgtgcc ggcgacgctt ctccatccga tcatggcact cgcgggatgg
      721 gtggttttgg cgatcgttct ttgtgtcaag gaccggcgct ggctgatcgc cgctggattg
      781 accgtcgcgg ccgcgatcat tgccggcgtt ttaggcctgc cgcttttcga tcggctcgtg
      841 cggctcatgg acccggattt gaaagcgctt gcgcaaagcc gcagcggtct tctgtttccg
      901 acgcattggt cgatcgcgtc ctacgcgccg attgttttgc aatccgcgac catcgcgagt
      961 gccgcgcgtt tcttcgaggg ccggcgaaag ctcatcttgc tcgcggtcgt tctcgccggc
     1021 attggcggca ttgcggcgca atccctgttc ggcgacgagc tgtccctgga gcttgtcatc
     1081 caggcacaat tctggcgcat gacatggctg ctggcagcca tcggcgccgt gtcgatggcc
     1141 atctgcgcga taaagctcat tcaaaacggg cgtgtcggct atttggttct cgcactgctg
     1201 accgcagcgt ggctctgcta tctggacttc atgatggtga gcgcgctctg cgcagcggcg
     1261 ctcgtcgtcc agttcagcgc cccccggtta gcgagcgtcg tgacgcggcg tgtcgtgatc
     1321 gtcgtgtgga tctgcgtggc ggcttacggg ttgttgctga acgtacttta cttcgagcag
     1381 tttggacgct ttttcgcgag ccttccggcg gaagggccgg gaggtttcac ctatttctgg
     1441 aacaattggc gctacatcgc ctttccgatc gcaggcggcg cactcgcact ggcattggcg
     1501 ccgcgcgccc gcgtcacatg ggcgatcgaa ggctttgcgg cgctcgctct tgcggccgtc
     1561 gcctggcatc tttgggacga caggtcggca tttcagaaga tcgtcgaccg caatcgccgc
     1621 cccgcgccgc tcgaacaatt gttggccggg aaagatggcg aaatcctctg gctcggcggg
     1681 cagacggaag cctggtttct gaccggtcgg ccgcaatggg cctcgccgca gcagggcgtc
     1741 gggacgatct tctcgcccaa gctcgcacgt gaatggcgcg gccacatgca atttcttatc
     1801 gacaatggat tggtcgagaa agaaggtctc gccatctcgc ataatttgtc gagtgcggaa
     1861 ctgcccgtag tgacggcgcc tgcggtcggg cgtctctgcg cccgtgccga cgcgccagcc
     1921 tggatcgtcg ctccggttta cgagggcgcg acattgccgg ctgcgctgaa ggcacaattt
     1981 tggactccgc cggtgcccaa tttccgcatg acggaagagc cggacggata tgtctggcac
     2041 cgcatttcga cctatgcggt tctcccctgc gtcggcgcgc agctctaaaa agccttgaaa
     2101 aagccagccg ggtgcggctc tctgcgcttg aatgaacgcc atttccgtcg ctttcacgcc
     2161 cttgctgccg cccgcgctcc tttggggctt tgcggctctc gcgctccttg tcaccgtctt
     2221 gtcgctgctt cgccgccggc gcggcgcgct gctgcgcgcg ctggggctcg gcctcgtgct
     2281 gctcgcgctg accgatccgt cgctcgtgcg ggaaaaccgc aaaccgctga aggatgtcgt
     2341 cgcgatcgtg gtcgatcaaa gcggtagcca gaccatcggc gaccgcatgg cgcagacgca
     2401 gaaagcgacc gccgatctga agcggtggct tgaggcgctc ggcaatatcg atgtgcggat
     2461 catcgagagc ggccgcaacg ataccgaaaa caacggcacg cggcttttct ctgcgctcaa
     2521 cgaggggctc gccgacgtcc cggcggagcg gatcggcgcc gtcttcatga tcaccgatgg
     2581 cgtcgtacat gacattccgt cgagcaccgc cgcactcggt ttcaaggcgc cgttgcatgc
     2641 gttcatcacc ggccatgaag gcgaacgcga ccggcgcatc gaacttttga ccgcgccgcg
     2701 tttcggtatc gtcggcaagg atgtgacgct ggaattgcgt gtcgacgata cgacgaacgg
     2761 cacggaaccg gtcgtccttc atgtccgccg agacgggaat tcgatcgccg atgtcaccgc
     2821 cgcaatcggc gagacgatta gcgtgcccgt gaagatcgag cacggtgggc ccaacgtcgt
     2881 cgaactcgaa gtcgaggcgc tgcccggcga actgactgcg ctcaacaaca aggccgtgct
     2941 gacgatcgac ggcgtgcgcg acaagctgaa agttctgctc gtttccggcg agccgaacca
     3001 gggcgagcgg atgtggcgca atctcttgaa gtccgacgcc aatgtcgatc tcgtccattt
     3061 caccattctg cggccgccgg aaaagcagga tgggacgccg atcaacgaat tggcgctgat
     3121 cgcttttccg acagcggacc tcttcggccg caagatcacc gatttcgatc tcatcatctt
     3181 cgaccgctat tccaatcagg cgatcctgcc cgcggtctat ctcgataata tcgtgcgcta
     3241 tgtgcacgac ggcggcgcgt tgatgatcgc ggcggggccg gattttgcat cgtcggacgg
     3301 gatctattat tcgccgctcg gcgcgttgtc gccggccgag ccgaccggcg acgtgatcca
     3361 gcaggcattt cagccagcga ttacggctgt cggcgccagg catccggtca cgcggggtct
     3421 acccggcgcg gacgaaacgc cgccagcctg gagccgctgg ttccgccagg tc
//
LOCUS       DASWOG010000112         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_39103,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000112 DASWOG010000000
VERSION     DASWOG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39103"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VGB93_02265"
     CDS             <1..858
                     /locus_tag="VGB93_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0144985.1"
                     /translation="RGFDAIERLKADYRWAADIQLCVFPQDGLTNLPGTDELIVQGLR
                     RGANLIGAAPRYDTDAKAQILRIFEIAREFDVDIDMHLDVGPTADHLDLYDVIELTKR
                     HGLGGRVTVGHMAKLSLLPPAELEKVGARLADAGINVTVLPATDLFLMGRKHDHAVHR
                     GVADANFLVKCGVNCSISSNNIMNPSTPYGDCSLIRIANLQANVLQVALPHELAECFH
                     MITDRSARILNIGNYGVEVGNPADIVVIDAPSPEKAVAEIRQPVAVFKRGRRTVAWNL
                     PQLLQPASA"
     gene            complement(931..1947)
                     /locus_tag="VGB93_02270"
     CDS             complement(931..1947)
                     /locus_tag="VGB93_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0144986.1"
                     /translation="MIETPPSFAVAPDKGHDVLAESLRAVRLTGSVFLDACFTAPFGI
                     ISPKRFDANSPMAHLRHVSVFHLIAEGECDVELANGERRKISAGDIILMPFADRHKFS
                     NGRALEAPLAEDVVRPGALKGIWTINHGGGGQETRMVCGFLESSEFLLTPVFRTLPPL
                     LVDRAEDDQVSALIKSTVGQILALTENAAPGSELMLGRLMELLFVEVLRRYATRLPAS
                     AKGRFAALNDPLVGRAIQSVHADPARRWTVEELAREAGASRTVLAERFHQVMGQAPIE
                     YITCWRMQLAAERIRAGNGSLAAIAADVGYDSEAAFNRAFKRVTGVTPGRWRDSGLIT
                     AVAS"
BASE COUNT          423 a          663 c          665 g          428 t
ORIGIN      
        1 cgcggtttcg acgcgatcga aaggctcaag gctgactacc gatgggcggc tgacatccag
       61 ctttgcgtgt ttccgcagga tgggctgacg aatttgccgg gaacggacga gttgatcgtc
      121 cagggcttgc ggcgcggcgc aaatctgatc ggcgcggcgc cacggtacga taccgatgcc
      181 aaagcacaaa tcctgcgtat tttcgagatt gcccgggaat tcgatgtcga tatcgatatg
      241 catctcgatg tcggtccgac cgccgatcac cttgatctct acgacgtgat cgaactcacc
      301 aagcggcatg gtctgggtgg ccgcgttacc gttggccaca tggcaaagct ttcattgttg
      361 ccgccggcag agctggaaaa agtcggtgcg cggctggccg acgccggcat caacgtcacc
      421 gtgttgcccg cgaccgatct tttcctgatg ggcaggaagc atgatcatgc ggtccatcgc
      481 ggcgtggccg acgcgaattt tctggtcaag tgcggtgtga attgttcgat atcgtcaaac
      541 aacatcatga atccgtcgac gccttacggc gattgctcgc tgatcaggat tgcgaacctt
      601 caagccaacg tgctgcaggt cgcgctgccc cacgagctgg ccgaatgctt tcacatgatt
      661 acggaccgct cggcacgcat tctcaacatt gggaattacg gcgtcgaggt cggcaatccc
      721 gccgacatcg tcgtgatcga cgcgccatcg ccggaaaagg ccgtcgcgga aattcgtcag
      781 cccgttgcgg tgttcaagcg tggcagacga accgtggcct ggaatctgcc gcaacttttg
      841 caaccggcga gtgcctgaaa taacatcgcg aaagcacacg gtccgcctgg caattccggc
      901 gtctccatcc agtctacggg tgacctgcag tcaggacgcg accgcggtga tcaggccgct
      961 gtcgcgccac cgcccgggcg tcacgccggt cacccgctta aaggcgcgat tgaacgccgc
     1021 ttcggaatcg tagccgacgt cggccgcgat cgcagcaagg ctgccgttcc cggcgcggat
     1081 acgttcggcc gcaagctgca tccgccagca ggtgatgtac tcgatcgggg cctgacccat
     1141 cacctggtga aatcgctcgg ccagcacggt gcgtgacgcg ccggcttcgc gcgccagttc
     1201 ctccaccgtc cagcgccgcg cggggtcggc gtgcacagac tggattgcgc gaccgaccag
     1261 gggatcgttg agtgcggcga accggccctt ggcgctcgcc ggcaaccgcg tcgcgtagcg
     1321 ccgcaacacc tcgacgaaca ataattccat cagccggcca agcatcagct cgctgccggg
     1381 ggctgcgttt tccgtcagcg cgagaatctg tccgaccgtg gatttgatca gcgcgctgac
     1441 ctggtcgtct tcggcgcgat cgaccaacag cggcggcaag gtgcgaaaca ccggcgtcag
     1501 caggaattcg gatgactcca gaaatccgca gaccatgcgg gtttcctgtc caccgccgcc
     1561 atgattgatc gtccagattc ccttcagggc tccggggcgg acaacgtcct ccgcgagcgg
     1621 cgcttcgagg gcgcgcccgt tggagaattt atgccggtcc gcaaacggca tcaggatgat
     1681 atcgccagcg gagattttcc tgcgttcgcc gttggcaagt tcgacgtcgc attcgccttc
     1741 cgcgatcaga tgaaacacgc tgacatggcg aagatgcgcc atcgggctgt tggcgtcgaa
     1801 gcgcttcgga ctgatgatgc cgaacggcgc ggtaaagcag gcgtcgagaa acaccgagcc
     1861 ggtcaggcgc accgcgcgca atgactcagc aagcacgtcg tgacccttgt ccggcgcaac
     1921 agcaaatgat ggcggtgtct cgatcattgt gggtttggct cttcaacaat acccatgagt
     1981 cactgatgca ggcgtttggt tcaacgcagt gcatccttac cgatggagat taaaatgatc
     2041 agaaattggg ctgcaaatgt ggccaatcag ggcgtcgctc tgatcgcgtc tttgttgatg
     2101 cgatcggcga ttaagtccct gctcgagatc gacgaacggt cccttcgggc cgcgggattg
     2161 tctcgcaccg acctcctcg
//
LOCUS       DASWOG010000113         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_94416,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000113 DASWOG010000000
VERSION     DASWOG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94416"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1445)
                     /locus_tag="VGB93_02275"
                     /pseudo
     CDS             complement(1..1445)
                     /locus_tag="VGB93_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020533.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
     gene            complement(1544..2188)
                     /locus_tag="VGB93_02280"
     CDS             complement(1544..2188)
                     /locus_tag="VGB93_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEY0144987.1"
                     /translation="MEEILASIRRIIADDHAAPPQAAAEPLTPASPPEIAPEPPLITE
                     APIELTRDHEAEQVEGWTGIPAAPAEPTPPLRSGFDFVPGREYRPLAARTPVAERPAF
                     SRDPAPRPAAEPPVERRENPRRLAPGPRSAPPTPVAPVAAEEALVSPATDAMVSQAFN
                     TLIASRFLQSNDVLGEMVREMLKPMLKAWLDDNLPILVERLVRTEIERVARGGR"
     gene            complement(2418..3917)
                     /locus_tag="VGB93_02285"
     CDS             complement(2418..3917)
                     /locus_tag="VGB93_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY0144988.1"
                     /translation="MSESWFRTGRCSQHRSRPTSYAGAPAPARVAFNVAALLAASLAF
                     TNAHAETMSDALARAYRTNPDLNQQRAGVRASDETVPQATAAYRPTVTATGQFGYSNL
                     SAHEPGTGVSSLGGIGAGGAITSGSFKASTDTGNIGATMTETIFNGNRNYNGVRQAES
                     NVLGARETLRNTEESVLQNGATAYMDVLRDTAILDLRKNNIIVLEEQLRQTRDRFNVG
                     EVTRTDVAQAASSLASARSDYFTAQANLETSIANYHEVIGVDPNRLEPARSVEGLLPH
                     TLDSAILVAMHEHPEVQAALHQVDAAELQVKIQEGALMPTVTLNGSVAQNYNDEGEPG
                     FRLFNATVLGSVSVPIYTGGLVDAQVRQAKEQLSQAELQMDLQRTQVRASVVSNWGLL
                     DTAKAVIISDDSAVKSAEIALEGVREEARVGQRTTLDVLNAQQTLLNARVSLVSAQRD
                     RVVASYATLASVGELSSDQLGLDIVRYDPTVHFNQVKDKWFGLRTPDGR"
     gene            complement(4164..>4344)
                     /locus_tag="VGB93_02290"
     CDS             complement(4164..>4344)
                     /locus_tag="VGB93_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY0144989.1"
                     /translation="QLRDNGRLLTILRSASPANQHASRAVLFEKNSGEISRRGLFDAP
                     APLLDAFRAEPAFVF"
BASE COUNT          828 a         1347 c         1311 g          858 t
ORIGIN      
        1 taggtggccc tcgctttctc tacagcatcg ccttctgaag tcgccgcgaa gatttggttt
       61 ttgccgccgt ctcccagttc gtaagcgagt cattattcgc tctcgtatgg cccataccac
      121 gctgggattt gatggccccc ccagagttgg cgcgagatgc accacggctg gatattgtcg
      181 agccattgaa agtaagtctt cgaccagttc tccgggatga acttcgtctt gccttcacgc
      241 accgcggaga gcgccggctg tgccagcact ttggcatcga cataccattg gtcggtcagc
      301 cgcggctcaa gcacgacgcc ggaacgatcg ccatgcggca cggcatgttg cgtcggctcg
      361 atcttttcga gcagaccgcg ctcttccatc atcacgacga caatcttacg cgccgcttcg
      421 cgggacttgc catcaagcgc atcaattgcc gcgttcaagt tgccagtctg ccagtcgacg
      481 ccatagagaa acgacagatt gccttgaagc tgaatgttag cttccgcgtc gagcacattg
      541 atcagcggca gattgtgcct cttgcccacc tcgaaatcgt tgaagtcatg cgccggcgtg
      601 atcttcaccg cgcccgtacc cttttcagga tcggaataat tatcggcgac gatcgggatc
      661 agccggccga caagcggcag ccgtacattc ttgccgacga gatgtgtgta acgctcgtca
      721 tcgggatgca cagcgacggc cgtgtcgccg agcatcgttt cgggccgtgt cgtcgcgacg
      781 acgatatagg tcgaaggatc atccgggttg aactctttgt ccgcaagcgg ataacggaaa
      841 taccagaggc tgcccttttc ctctttctgc acgacttcga gatcggagat cgcggtgagc
      901 agcttcgggt cccaattgac gaggcgcttg tccttgtaga tcagcccgtc gcgatagagt
      961 tgcacaaaga ctttcacgac agccttcgac agcccctcat ccatcgtgaa gcgctcgcgc
     1021 gaccagtcgc aggaggcgcc gaggcgcttt aattgcgcga cgatggcgcc gcccgattcc
     1081 tctttccagc gccagacttc ttcgaggaat ttctcgcgcc ccaattcgcg gcgccccggc
     1141 cgttgccgct ccgccaattg ccgctcgacg accatctgcg tcgcgatgcc ggcgtgatct
     1201 gtgcccggct gccagagcac gtctttgccg cgcatgcgct cgaagcggca aaggatgtcc
     1261 tgcagcgtat tgttgagcgc gtggcccatg tgcagcgagc cggtgacgtt cggcggtggg
     1321 ataacgatcg tgaaagtctc ggctccggcc cgctccggcc gcccggcgcg aaacgcacct
     1381 gcctcttccc aaagcgcgga aatccgccgc tcgacatgcg cagggtcgaa agtcttgtcc
     1441 atcatcccga aaaaccagaa agctcgcgca ccaagcgcca gcaagagcgc ctaaaagccc
     1501 aagcggcgcc ttgaagtcaa cgcaggtttg aaaaccgaaa ggctcagcgg ccgccgcggg
     1561 ccacgcgctc gatttcggtg cggaccagcc gttcgacaag gatcggcagg ttatcgtcga
     1621 gccaagcctt gagcatgggc ttgagcatct cgcgcaccat ttcgccaagc acgtcgttcg
     1681 attgcaggaa acggctggcg atcagcgtat tgaacgcctg ggacaccatc gcgtcggttg
     1741 cgggcgagac gagtgcctct tcggccgcca caggcgcgac cggagttggc ggcgcggagc
     1801 gcgggcctgg cgcgagacgg cgcggatttt cccgccgttc aaccggcggc tccgcggcag
     1861 ggcgcggcgc ggggtcgcgc gagaaagcgg gccgctcagc aacgggcgta cgcgccgcca
     1921 gcggacgata ttcgcgcccg gggacaaaat cgaaaccgct acgcaggggc ggtgtcggtt
     1981 cggcgggcgc ggccggaata cccgtccagc cctcgacctg ctcggcctca tggtcccgcg
     2041 ttaattcaat cggcgcctca gtaatgagtg gtggctctgg cgcgatctcg ggcggcgagg
     2101 ccggcgtcaa cggctcggcc gcagcttgcg ggggcgctgc atggtcgtcg gcgatgatgc
     2161 gacggatcga cgcaagaatc tcttccatcg aaggctcgta ggcctttcta tcggccgcaa
     2221 ttttgtcttg cgaaagggcg tttggcgcgt tcatgccctg ggcactttca aaatcgagcg
     2281 gatcagcccg cgaatccccg caggctttcc acctcaaatc agattcgtgc aattagaccg
     2341 caagtcgtcg cttttcgata gaggcgattt cgttattcac atcgatcgaa gcggctcaaa
     2401 tctcaagcgt tcgtgactca gcgcccgtct ggcgtccgca gcccgaacca cttgtccttg
     2461 acctggttga aatggacagt cggatcgtag cgcacgatat cgaggccgag ctgatctgac
     2521 gacaattcgc cgaccgatgc gagggttgca taagacgcaa cgacacggtc gcgctgcgct
     2581 gagacgagac tgacgcgcgc gttcagaagc gtctgctgcg cattcagcac atcgagcgtg
     2641 gtccgctggc cgacgcgcgc ctcttcacgc acgccttcga gcgcgatttc tgccgatttc
     2701 actgccgaat cgtccgagat gatcacggcc ttcgcagtgt cgagcagccc ccagtttgag
     2761 acgacagagg cgcgcacctg cgtgcgttgc agatccatct gcagctccgc ctgcgacagc
     2821 tgttctttgg cctgccgaac ctgagcatca accagaccgc ccgtgtagat cggcacggag
     2881 actgagccga gcacggttgc attgaaaaga cggaaacctg gctcgccctc atcattataa
     2941 ttctgcgcga cggagccgtt aagtgtcacc gtcggcatca gcgcgccttc ctgaatcttg
     3001 acctgaagtt cagcggcatc gacctgatgc aaagccgcct gcacttccgg atgttcgtgc
     3061 atcgcgacga ggatcgcact gtccaacgtg tgcggcaaca aaccttcgac cgagcgcgcc
     3121 ggttcgaggc ggttcggatc gacgccgatg acctcgtggt aatttgcgat gctcgtttcg
     3181 agattggcct gcgcggtaaa atagtcggat cgcgcggagg caaggctcga tgcggcctgg
     3241 gcaacgtcgg tacgtgtcac ttcacccacg ttgaagcggt cacgcgtctg gcgcaattgc
     3301 tcttcgagaa cgatgatgtt gttcttgcgc agatcgagaa tggcagtatc gcgcagcaca
     3361 tccatatagg cggtcgcgcc attctgcaga acgctttctt cggtattgcg caaggtctcg
     3421 cgcgcgccga gcacattgct ctccgcctga cgcacgccgt tgtaattgcg atttccgttg
     3481 aagatcgtct cggtcatcgt tgcgccgatg ttgccggtgt cagtcgaggc tttgaaactc
     3541 ccggacgtga ttgcacctcc cgctccgatc ccccccaacg acgaaacgcc ggtgccgggc
     3601 tcgtgcgccg agaggttgct gtagccgaat tgccccgtcg cggtcacagt gggccggtag
     3661 gctgcggtcg cttgcgggac ggtttcatcc gatgcgcgca cacccgcgcg ttgctgattc
     3721 aagtctggat tggtgcgata ggcccgcgcc aacgcatcgg acatcgtttc cgcatgggcg
     3781 ttggtgaaag cgagagacgc ggcgagaagc gccgcgacat tgaaagcaac gcgcgccggc
     3841 gcaggcgcgc cagcgtaaga ggtcgggcgg ctccggtgct gactgcaccg gccggttcga
     3901 aaccagcttt cgctcaaacc ttgaaactcc acattccacc gacacagcgc gtcgtgacga
     3961 cgccaccgtc gattgcggac ctttggcgca tgcacaaagt ccggcccttt ggtattaagc
     4021 ccctcccccc gtcagcgcca agcctgtggc gctagaaact ggccgggtgg agcaaaaatg
     4081 cgacacattc acaaggcggt acggataacg ggcattatcg cagatgtgga taaattgctc
     4141 cgccttgaaa cggtgcgccg gccctagaaa acgaaagctg gttccgcgcg aaacgcatcg
     4201 agcagcggag ccggggcgtc gaacaggccg cgccgggaaa tctcaccgga atttttctcg
     4261 aaaagaaccg cgcggcttgc atgctggttt gccgggctgg ccgatcgcag gatcgtgaga
     4321 agccgcccgt tgtcgcgcaa ttgc
//
LOCUS       DASWOG010000114         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_131074,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000114 DASWOG010000000
VERSION     DASWOG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131074"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGB93_02295"
     CDS             complement(<1..210)
                     /locus_tag="VGB93_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0144990.1"
                     /translation="MTMRNAARVLVDQLVVNGVTHAFCVPGESFLPVLDALRDSPIEV
                     VICRQEGGAAMMAEAVGKATGRPGVC"
     gene            complement(207..1100)
                     /locus_tag="VGB93_02300"
     CDS             complement(207..1100)
                     /locus_tag="VGB93_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA acyltransferase"
                     /protein_id="HEY0144991.1"
                     /translation="MVNTVSSVRPATLACRQIDEADLSAIADLLTRGFPGRRQSYWMQ
                     GFTRMAALAVPPTYPRFGYMMENDGKPVGVILLLYRQMSDETSAVYIRCHLSSWYVEP
                     SMRSYASMLVSFALKRPDVTYINISPAPPTWPVVEAQGFKRFSEGQFFALPALSSKGF
                     GLSVVKVPSAAEINLPAAERAILADHAQFGCLSLVCGTENGGLPFVLAPFGIRAGRMP
                     LPFMRLIYCRDMADFLLCAGALGRYLLRHGVLSVLLDVKDTTPALPGIYSQKLGRKYF
                     KGPHPPRLGDLAYSEYAVFGP"
     gene            1177..1425
                     /locus_tag="VGB93_02305"
     CDS             1177..1425
                     /locus_tag="VGB93_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0144992.1"
                     /translation="MSIRLTIYSLIEQIAAEQKVKIQPLRDDLLLSESGLDSLCFAIL
                     VARLEDKLGYDPFATAADAGFPVTLGDFVRFYENGATA"
     gene            1409..>1883
                     /locus_tag="VGB93_02310"
     CDS             1409..>1883
                     /locus_tag="VGB93_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0144993.1"
                     /translation="MARQPEPQTLRQVLAGAAGTRRMLWDAHGALALADLAAGSVFRD
                     GDMSSRSIVLATHAQLAAAQALVALDGVARRIIVCPPDLPLAHLAAIAAEAGAEAIVT
                     DRDEADFAGHDLPSRIACDFSPGSHEAAAPIETEWVLLTSGTTGVPKMVVHSLAAL"
BASE COUNT          415 a          578 c          531 g          359 t
ORIGIN      
        1 acaaacgccc gggcgtcccg tcgctttacc gacggcttcc gccatcatcg ctgcgccgcc
       61 ctcttgccgg catatgacga cctcaattgg gctgtcgcgc aaggcatcga gcaccggcag
      121 aaagctttcg cccggcacgc aaaaagcatg ggtgacgcca ttgacgacaa gctgatcgac
      181 aagaacccgc gccgcatttc gcatcgtcat ggcccgaaca ccgcatattc actgtaagca
      241 agatcgccga gacgtggcgg atgcggcccc ttgaaatatt tgcggcccaa tttctgtgaa
      301 tagataccgg gcaaagcggg cgtcgtatct tttacatcaa gcaacacaga caagacccca
      361 tgcctcagca gatagcggcc gagcgcgccc gcgcaaagaa ggaaatccgc catgtcacgg
      421 caatagatga gccgcatgaa aggaagcggc atgcgtccgg cgcggatgcc gaacggtgca
      481 aggacgaacg gcaaaccgcc gttctccgta ccgcagacaa ggctaaggca cccaaactgt
      541 gcatggtccg ctaaaatcgc tcgttccgcg gccggcaaat tgatctccgc agcggacgga
      601 actttcacga cgcttaagcc aaaccccttc gaactcaggg ccggaagggc aaagaattgc
      661 ccctcgctga agcgcttgaa cccctgcgct tcgacgaccg gccacgtcgg cggcgcgggg
      721 gagatgttga tataagtgac atcaggccgc ttaagcgcga atgaaaccag catcgacgca
      781 tagcttcgca tcgacggctc gacataccag cttgaaagat ggcaacggat ataaaccgcg
      841 ctggtctcgt cagacatttg gcgatagagc aacaagatca caccgaccgg cttgccgtcg
      901 ttttccatca tatagccgaa gcgcggataa gtgggtggca cggccagtgc cgccatccgc
      961 gtgaaacctt gcatccagta agattgccgc cggcctggaa aaccacgcgt cagcagatcg
     1021 gcaatcgcac tgaggtcagc ctcatcgatc tgtcggcaag caagagtcgc aggacgtacc
     1081 gacgagacgg tattcaccat gaggccccga ggcgaattga ataacttttg aatttaatgt
     1141 aatgcaccac attggccaag tgaaggtgcc cgctctgtgt ccattaggtt aacaatatac
     1201 tcgctgatcg aacagatcgc ggccgaacaa aaggtcaaaa ttcagcctct gcgcgacgat
     1261 ctgcttttgt ccgaatcggg cctcgactcg ctttgtttcg ccatccttgt tgctcgactt
     1321 gaggataagc ttggttacga ccctttcgcg acggcggccg acgcaggttt tccggtgacg
     1381 ctgggcgatt tcgtcaggtt ctacgagaat ggcgcgacag cctgaaccgc aaacattgcg
     1441 tcaggttctc gccggtgcgg cgggcaccag acgcatgttg tgggatgcac atggcgccct
     1501 cgcactggcc gatctcgccg caggtagcgt attccgcgac ggcgatatgt ccagccgctc
     1561 aatcgtcctg gcgacgcatg cgcagcttgc tgccgctcaa gcgcttgtcg cactcgatgg
     1621 tgtggcgcga cgcatcattg tctgtccgcc ggacctgcct cttgcccatc tcgctgcgat
     1681 tgccgccgaa gccggggccg aagccatcgt cacggatcgc gacgaggctg attttgccgg
     1741 ccacgacctg ccctcacgca tcgcctgcga tttttctccg ggctcgcacg aggccgccgc
     1801 gccgatcgaa acagaatggg tgctcttgac ctctggcacg acgggtgtgc cgaaaatggt
     1861 cgtccattcg cttgccgcgc tga
//
LOCUS       DASWOG010000115         6774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_259359,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000115 DASWOG010000000
VERSION     DASWOG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259359"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGB93_02315"
     CDS             <1..328
                     /locus_tag="VGB93_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0144994.1"
                     /translation="DALEAQAAPIAAGPWLFPADSDSGHLSRQVFARELKTIAARAGI
                     SVARVSPHVLRHAFASHLLQNGADLRVVQELLGHADISTTQIYTHALDARMKAMVRDL
                     HPLNDD"
     gene            complement(325..1083)
                     /locus_tag="VGB93_02320"
     CDS             complement(325..1083)
                     /locus_tag="VGB93_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0144995.1"
                     /translation="MVQSAVFVGDMIPETPSAGRVLIVLHQRDSSPGRLGSLLSARGF
                     DLDIRRPRFGEPLPETLADHAGAIIFGGPMSANDDEDWLRREIEWIKVPLTENKPFLG
                     ICLGAQMLARHLGHRVYTHPHGKVEVGYYPIQPTDHGHNVCECPFPERVYQWHREGFD
                     LPGGATLLASGEDFEAQAFRYGAMAYGFQFHPEVTFGMMCRWSIKGADKMVLPGAQPR
                     HLHIEGWHRHDAPVARWTGAFLSAWAARVPMSSV"
     gene            complement(1126..1623)
                     /locus_tag="VGB93_02325"
     CDS             complement(1126..1623)
                     /locus_tag="VGB93_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0144996.1"
                     /translation="MFDRLKNFVAEFAGPSERRDFSSDDFRLAAVALLVHLAGADGRI
                     DESERKRLRELIESRFGLDAETTTRLIELAEAQDRQAVDFFHFTHALNKVLDQEGRQK
                     IITMMWEIAFADSTVSEVEESIVARIADLLGVSSQDRVRLRRQVASDVASGTAFAGPW
                     SPAMP"
     gene            complement(1652..1972)
                     /locus_tag="VGB93_02330"
     CDS             complement(1652..1972)
                     /locus_tag="VGB93_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144997.1"
                     /translation="MRFFLASVFLFLTSIAAQAAGTDIGKPPIFGDGIACDASLLPWR
                     SVWYGHFSGGAATYAPNSPAAQVDWHDEKLCFPSRHACLRWQHGERRLYRNIRGDWTC
                     LPLR"
     gene            complement(2065..2307)
                     /locus_tag="VGB93_02335"
     CDS             complement(2065..2307)
                     /locus_tag="VGB93_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0144998.1"
                     /translation="MDLPERGTINWLHVSTLIAVAILVGTEMVGASWAAGWALGGLLQ
                     LSPVISHILEGMFALCGVVLLYYFMRTAISNESVRN"
     gene            complement(2466..2753)
                     /locus_tag="VGB93_02340"
     CDS             complement(2466..2753)
                     /locus_tag="VGB93_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY0144999.1"
                     /translation="MTHSTESPTRSSTRGFASMDPEKQRAIARKGGESVPHEKRSFSQ
                     NPGLAAQAGRKGGQSVSPNKRSFSQNHQLASEAGRKGGHASHGGAKQPVKN"
     gene            2962..3285
                     /locus_tag="VGB93_02345"
     CDS             2962..3285
                     /locus_tag="VGB93_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145000.1"
                     /translation="MADLKSIGDQATSDLTEDLTALRDDISRLSASVLELVQQQAATT
                     KSQVFDAVDGARQRFADQSAEAKDRLGALTTDLESTIERNPFTAIFAGALAGFLIGIL
                     VRPTR"
     gene            3282..3860
                     /locus_tag="VGB93_02350"
     CDS             3282..3860
                     /locus_tag="VGB93_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145001.1"
                     /translation="MNNFLARQLREITATVEAAGGRFVASIIVSLIGALALLAGLAFL
                     AVALFLKVAALAGAVYAALAVGGAFILVALIALLIVSLRRPVKPAAPKHVTEQQKVAA
                     VAAAEAATEKVERRAVLSANIDETLAPVLDALHQANRQPEELILRVGAELTKQTGGFG
                     LIALAVGAGFLAARQISGDKKISGEKKPPPAL"
     gene            complement(3881..4705)
                     /locus_tag="VGB93_02355"
     CDS             complement(3881..4705)
                     /locus_tag="VGB93_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145002.1"
                     /translation="MSVSKAISAHVPYLRRFARALTGTQAGGDAYVLATLEAIVAEPA
                     AFSDNADIRVELYRLFLKVWESMPINGFVDEAPTSPDEKGALRNLEAIPLQPRVAFLL
                     SSLEGFDTSEAARILECSPEEATALIDRAGKEISRQIHCDVLIIEDEPLIAIDIQMLV
                     EELGHRVVAIARTRDEAIAAAKTSTPGLILADIQLADGSSGLDAVNQILNDFSLPVIF
                     ITAYPERFLTGTPPEPAFLITKPFGVDSLKAVISQALFFDRRSHRKAPPETKAALV"
     gene            5135..5332
                     /locus_tag="VGB93_02360"
     CDS             5135..5332
                     /locus_tag="VGB93_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEY0145003.1"
                     /translation="MLPVSRRKQAPGPEIVEQIGHRLASVYNSVLAQPIPDRFLDLLA
                     QLEAGESQGEPGEKSVKEEKR"
     gene            5329..5940
                     /locus_tag="VGB93_02365"
     CDS             5329..5940
                     /locus_tag="VGB93_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0145004.1"
                     /translation="MTGVPSNTAPQPRQASAQIKADLIAVIPNLRAFAVSLCGNPDRA
                     DDLVQETLVKAWGNLDSFVEGTNLPAWLFTILRNFYYSEYRKRRREVSDPDGIIATKL
                     ATAPAQNGHMDLRDLHDALQQLQGDQREALILIGASGLSYEEAANICGCAIGTMKSRV
                     NRARNRLADILAIHSTSELGHEEDWPLNDPMAVSHHLSKTGSD"
     gene            complement(5980..6408)
                     /locus_tag="VGB93_02370"
     CDS             complement(5980..6408)
                     /locus_tag="VGB93_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006071948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145005.1"
                     /translation="MPPIRQPSFADVLEDVAHAPHDEFGEDESSPAPVNQDWLFHIFV
                     EAAEAVATPKRHDAYRENAGDIFQMPLFDATADEDSIAAELDLAAARNLDDLARIRRS
                     FARRNHPDKLHPRLAEIATARMKTANMLIDRREHELKQRS"
BASE COUNT         1387 a         1981 c         2096 g         1310 t
ORIGIN      
        1 tgacgcgttg gaagcgcagg cggcgcccat cgccgccggg ccctggctat tccccgccga
       61 cagcgacagc ggccatttga gccggcaggt ctttgcccgt gaattgaaaa cgatcgccgc
      121 gcgggcgggg atcagcgtcg cgcgcgtcag cccgcatgtg ctgcgccatg ccttcgcgag
      181 tcatcttttg cagaatgggg cggatctgcg tgtcgttcag gaattgctcg gacacgccga
      241 tatttcgacg acccagatct atacgcatgc gctcgacgcg cggatgaagg caatggtgcg
      301 cgatctgcac ccgctgaacg acgattagac ggaggacatc ggcacgcgcg ccgcccaggc
      361 gctcaggaag gcgcccgtcc accgcgcgac cggcgcgtca tgacggtgcc agccttcgat
      421 atggaggtgg cgcggctggg cgccgggcag aaccatcttg tccgcgcctt tgatgctcca
      481 acggcacatc atgccgaagg tcacctcggg atggaattga aagccgtagg ccattgcgcc
      541 gtagcgaaag gcctgggcct cgaaatcttc ccctgaagcc agtagggtcg cgccgcccgg
      601 cagatcaaag ccttcgcggt gccattgata gacccgctcg gggaacgggc attcacagac
      661 attgtggcca tggtccgtcg gctggatcgg ataatagccg acttcaacct ttccatgcgg
      721 atgggtatag acgcggtgac cgagatggcg ggccagcatt tgcgcgccaa ggcagattcc
      781 gagaaagggc ttgttctcgg tcagcggtac tttgatccac tcgatctcgc ggcgcagcca
      841 gtcttcgtcg tcgtttgcgc tcatgggacc accgaaaatg atggcgccgg catgatcagc
      901 gagagtttcc ggcaagggct cgccgaatcg cgggcgacgg atgtcgagat cgaagccgcg
      961 tgcgcttagc aggctgccga ggcgcccggg ggaagaatct cgctgatgta gaacgatgag
     1021 caccctgccg gcgctcggcg tttcaggaat catgtcgccc acaaagactg ccgattgaac
     1081 catttctccg gaaatgatcg gcaagactaa ggcagaggtc cttgcctaag gcatcgccgg
     1141 tgaccacggc ccagcaaatg ccgtgcccga ggccacgtcc gacgcgacct gacggcgcag
     1201 ccgcactcgg tcctggctcg aaacgccgag aagatcggca atgcgcgcga cgatactttc
     1261 ctcgacttcg gaaacagtgc tgtcggcgaa agcgatctcc cacatcatcg tgatgatctt
     1321 ttggcggcct tcctgatcga ggactttgtt cagggcgtgg gtgaaatgga agaagtcgac
     1381 cgcttggcga tcctgcgcct cggcaagctc gatcaaacgc gtggttgtct ccgcatcgag
     1441 gccaaagcgg ctctcgataa gctcacgaag ccgtttacgc tccgattcgt cgattcgacc
     1501 gtctgccccg gcgagatgga cgaggagcgc aacggcggcg aggcgaaaat catccgacga
     1561 gaaatcgcgc cgctccgatg gaccggcaaa ttccgcgacg aaatttttaa ggcgatcgaa
     1621 catggctctc cgttacgtca gcgcggtcgc atcagcgcaa aggcaggcac gtccagtcgc
     1681 cacggatgtt ccgatagagg cgccgttcac catgctgcca acgcaggcac gcgtgccgcg
     1741 atggaaagca cagcttttcg tcatgccaat cgacctgcgc tgctggcgaa ttcggcgcat
     1801 acgtggccgc gccgcctgag aagtgcccgt accaaaccga gcgccagggc agcaggctcg
     1861 cgtcgcaggc aattccgtcc ccgaaaatgg gtggtttgcc gatgtcggtg ccggcggcct
     1921 gcgccgcaat cgacgtcaga aaaagaaaga ctgaagcgag gaagaaacgc atgccaaggg
     1981 gttccgcagc ggagcaagtc acgtcactat aattgccgcg ccgcgacttg gtaagtgcgg
     2041 cctagttgcg aagtaagcgc gggcctaatt gcgaacggat tcattgctga tggctgtgcg
     2101 catgaaataa tagagaagca ccacgccgca cagcgcaaac atgccctcaa gaatgtggct
     2161 gatcaccggg gatagctgca aaaggccgcc gagcgcccac ccagcagccc aagacgcacc
     2221 aaccatctcg gtccccacaa gaatggcgac cgcgatgagc gtcgaaacat gcaaccagtt
     2281 gatcgtgccg cgctcaggca aatccaaaac ctatccgctc ctgccgcaca ggcagcaagg
     2341 cggaaataga ccatcggctt gccaggggca agctggattg atgaacgctt cgcccgcatt
     2401 gccagcaggt gcgtcaggcg cacgctgctc aatgtttcgc gactaattga cccgacgggc
     2461 ttcgatcaat tcttgacggg ctgcttggcg ccgccatggg acgcatggcc acccttgcgg
     2521 ccggcttcgg acgcgagctg gtggttttgg gagaagctgc gcttgttcgg gctgacgctt
     2581 tgtccgccct tacggccggc ctgcgcggca agtccggggt tttgcgagaa gctccgcttc
     2641 tcatgcggca cgctttcgcc gccctttcga gcaatcgcgc gctgcttctc agggtccatc
     2701 gaggcgaacc cccgcgtcga cgatctcgtt ggactctcag tggaatgtgt catgtattgc
     2761 cctcttcgat aaggcctaga tgtgggtgag caacgcaccg ttcgggcgaa ggttccaaat
     2821 ttgatggtcg aataagcctt ttgtcgcagt cccgcgaaac ggggccgtta gcgttaaatt
     2881 tctattaggc gccaaggggt cgagacgatt tgccatttcg ggcgttaaag tggacttcaa
     2941 acatgggaga tggagatgac catggcggat ttgaaaagca tcggcgatca ggcgaccagt
     3001 gatttgaccg aggacctgac ggctctgcgc gacgatattt cgaggctgtc ggcctcggtg
     3061 ctggaactgg tgcagcaaca ggcggcgacg accaaaagtc aggttttcga cgccgtcgat
     3121 ggcgcgcgtc agcggttcgc ggatcaatcg gccgaggcca aggatcggct gggcgcgctg
     3181 accaccgacc ttgaatcgac gatcgagcgc aatcccttca cggcgatctt tgccggcgcg
     3241 ctcgcgggct tcctcatcgg tattctggtg cgcccgaccc gatgaacaac tttttagcgc
     3301 gccaattgcg cgaaatcacc gcgaccgtcg aggcggctgg cggccggttc gttgcgagca
     3361 ttatcgtgtc ccttatcggg gcgctcgctc tgctggccgg gctcgccttt ctggcggttg
     3421 cgctgtttct caaagttgcc gcactcgctg gcgccgttta cgcggcgctt gcggtgggcg
     3481 gcgcgttcat tttggtggcg ctcatcgcgt tgctgattgt gagtctccgg cgccccgtca
     3541 agccggcggc gccaaaacac gtcacagaac agcagaaggt tgccgccgtg gcggcggcag
     3601 aagcggcgac tgagaaagtg gaacggcgcg cggtgctctc cgccaatatc gacgaaaccc
     3661 tcgcgcccgt cctggacgca ttacaccagg ccaacaggca gccggaagag cttatcctgc
     3721 gggttggcgc tgaactcacc aaacagaccg ggggcttcgg cctcatcgct ctcgcggtgg
     3781 gcgcggggtt tctggcagca cgccaaatct ctggcgacaa aaaaatctcg ggtgagaaaa
     3841 agcccccacc ggccttatag ccggcggagc tctgacactt tcagaccaag gcggccttag
     3901 tctccggcgg cgctttgcgg tgcgaccggc ggtcgaagaa cagcgcctga ctgatgaccg
     3961 ccttgaggct gtcgacgcca aatggcttgg tgatgagaaa agccggttcc ggtggcgtgc
     4021 cggtgaggaa gcgttccgga taggcggtga tgaaaatcac cggcaacgag aaatcgttga
     4081 ggatctgatt gacggcatcg aggccggagc ttccgtccgc caattgaata tcggcaagga
     4141 tcaggccggg ggtcgaggtc ttcgccgcgg cgatggcttc gtcgcgggtg cgggcgatcg
     4201 ccacgacgcg gtggcccaat tcttccacta gcatctgaat gtcgatggcg atcagcggct
     4261 cgtcctcgat aatgagaaca tcgcaatgga tctgccgcga gatttcctta ccggcgcgat
     4321 cgataagcgc cgtcgcctcc tccggcgagc attcaagaat gcgggcggct tcggacgtgt
     4381 cgaaaccttc aagcgaactc aaaagaaagg cgacgcgcgg ctgtaacgga atcgcctcga
     4441 ggtttcgcaa cgcgcccttt tcgtccggcg atgtgggggc ctcgtccacg aaaccattga
     4501 ttggcatcga ctcccagacc ttcaaaaaaa gacggtagag ctcgacgcgt atatctgcgt
     4561 tgtccgagaa agccgctggc tcggcgacaa tggcctccag cgtcgcgagg acatatgcgt
     4621 ctccgccggc ctgggttccg gtcaaagccc gggcgaagcg ccgcaaataa ggaacatgcg
     4681 cggatatcgc tttcgatacg gacattctca aattcctcca gaccaacttt acgcggaagc
     4741 taatgcttgg gaagaaattt agttcccgtc aaaggaacct ttgtcaaaac atggcgtttc
     4801 aagtcggggg gtagactaat cataatatcg ctaggtctga tgccgcccga aattcttaaa
     4861 cacctcggtt tcacgtaaaa acgaccggat gagagtaaag ggctcaggtt gcccgtgtag
     4921 aggcggtttc cgccaatggg gttgggtttc gccgatggat cagaacggaa aacgaaataa
     4981 gggcacgcca gtgacgagac ctataaacct taaaccccgg aacgttgctt tgtcgtcacg
     5041 ccattcggac ccaccccaat ctgatgaagc caaccgcggg gactcttcag cgccatcgct
     5101 acatcaagag gccaagggtt tggcgggcgt gagcatgttg ccggtttcgc gccgcaagca
     5161 ggctcctggc cccgagatcg tcgagcagat cggccatcgc ctcgcgtccg tttacaattc
     5221 ggtgctcgcc cagccgattc ctgaccgttt tctcgatctt ctggctcagt tggaggcggg
     5281 tgagagtcag ggcgagccgg gcgagaaatc cgtgaaagag gagaagcggt gacaggtgtt
     5341 ccgagcaaca cggcgccgca gccgaggcag gccagcgcgc agatcaaagc ggatctgatc
     5401 gcggtcattc cgaatttgcg tgctttcgcg gtttctcttt gcgggaatcc ggaccgtgca
     5461 gacgatctcg tgcaggaaac gctggtcaag gcgtggggca atctcgattc tttcgtcgag
     5521 ggcaccaatc tacccgcgtg gcttttcacc atcctgcgga atttctacta ttccgaatat
     5581 cgcaaacgcc ggcgcgaggt ctccgatccc gacggcataa tcgcgacgaa gctcgcaacg
     5641 gcgccggcgc agaacggcca tatggatctg cgcgatttgc acgacgcctt gcagcaattg
     5701 cagggtgatc agcgcgaggc gttgatcctg atcggcgcgt cgggcctttc gtacgaagaa
     5761 gcggcaaaca tttgcggctg cgccatcggg acaatgaaga gccgcgtcaa ccgcgcgcgc
     5821 aaccgtctcg cagatatcct cgcgatccat tcgacgagcg aattgggcca cgaggaagat
     5881 tggccgctca acgatccgat ggccgtcagc catcacctgt cgaagacagg tagcgattag
     5941 cgttttcgcg tgtccgtcat ttgaacggga aagcgcgtct tagctgcgct gctttaattc
     6001 gtgttcccgc cggtcgatca gcatattggc ggttttcatc cgcgctgtcg cgatttctgc
     6061 gaggcgcgga tgcagcttgt ccgggtggtt gcgacgcgcg aaactacggc gaatgcgggc
     6121 aagatcgtca agatttcttg cggccgcaag atcaagctcc gccgcgatgc tgtcttcatc
     6181 ggctgtcgcg tcgaagagcg gcatttggaa gatatcacca gcgttttcgc gataagcgtc
     6241 gtgtcgtttc ggcgtggcga cggcttcggc agcctccacg aaaatatgaa acagccaatc
     6301 ctgattgacg ggagccgggg agctttcgtc ctcgccgaac tcatcatgcg gcgcatgtgc
     6361 gacatcctcg aggacatcgg cgaaagacgg ctgcctgatc ggcggcatga gaaactccgg
     6421 ctgattccgg gcgcattcaa acgactggcg cttaagattg aattaactac gccatgtcac
     6481 gagcgtcgcc gcggtacgca tccgccagaa cccggccttc cgtaacgcgg tgtaacagct
     6541 tttgtcgcaa aggatttgtt cggtggattc acaagagcgc gatgtgacag catggagatg
     6601 accgaagccg cgaccgaagc gctgcctgcg gcgacgcgaa gcaagcctct gccggtttcg
     6661 attctgattc tcgcggccct ggcgctgggg ctcgtgatgt catccgccga tcttcacagc
     6721 gtatggcagc acggcacgtt tgccgattca gacgacgcga tgcagctcgt cgag
//
LOCUS       DASWOG010000116         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_186072,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000116 DASWOG010000000
VERSION     DASWOG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186072"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGB93_02375"
     CDS             <1..275
                     /locus_tag="VGB93_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145006.1"
                     /translation="DAEPPPAPAPLKPPRHARHSAQQPPQDMQNVPVDISPPPGFQAP
                     PDNGGDNQDRLGPGTPNPSPDNGGDDDQAPPPQVPGMLPPDNNNNN"
     gene            complement(272..964)
                     /gene="aat"
                     /locus_tag="VGB93_02380"
     CDS             complement(272..964)
                     /gene="aat"
                     /locus_tag="VGB93_02380"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0145007.1"
                     /translation="MSRPRQEFEITPDILMRAYSIGLFPMAESAEDQNLFWIDPEARG
                     VFPLDKMIISKKLARLVRSNRFEVRIDYDFEAVIDACAGVGEGREKTWINQRIKKLYR
                     QLFDLGRVHTVEAWRGGVLAGGLYGVALGAAFFGESMFHRQTDASKVALIHLAGRLAA
                     GGFHLLDTQFVTPHLQSLGAIEVSKETYRLALADAVAHQGDFLVWPKNQPVMGREILA
                     ALEGAGNPASEI"
     gene            1246..2661
                     /locus_tag="VGB93_02385"
     CDS             1246..2661
                     /locus_tag="VGB93_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0145008.1"
                     /translation="MAALAGILASSCVRDQPVPLSPAVTAASLESRSLGDPRLRQFVI
                     AATRPEAAPDAPIRWDLASLTAAALYYHPDIALAEAKLAGAKAAVVTANEHPNPVLNF
                     TNIVGQGLVPEAIPIGAAPLTIGPAIDFMIDAFGRREARTAEAQRLEQAARWDLAAAG
                     WQVRSRVRAALLTLWATERRLALTQHRLALQNQLVELLEHRQTAGEASALDVSRERVN
                     RAEVTLAMHDLDLAVAEARVQVATAIGVPDQALDGITLETAVFDRPPALARAATSNAW
                     RREALTRRADIQASLATYSASQSALKLAIVGQYPDITLSPGYSYEYGINQYQLNVVST
                     LPIFNQNQGAIAEAIAKRREAAARFIALQAQIIGELDYATVAYRKSTQSLTSAEAMQA
                     DETHRARQVEKSFSAGQADRPTLVSTQVEAAITALTNFDAMLQQREALGALEDALERP
                     LYGPDILLILPEAQLPPRSSS"
     gene            2685..3698
                     /locus_tag="VGB93_02390"
     CDS             2685..3698
                     /locus_tag="VGB93_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145009.1"
                     /translation="MALLLLAFAVGGVLGSFYLIPSHADEDSDNDNPNPAVRLTIKDG
                     VPTLTLTAEEQQNAGVIAAKLDVAPPRDYMRGFGTVLDPAGLADLENQYLEAETQIVA
                     AKSRLGSSQAAYQRAQVLNKDQENISTAQLQSARSSFDVDTNALAAAQAQSAAIIANT
                     RQTWGETIAKALASGGPEINDLLARNHYLVRVTLPPDVTISTPPSIASALYGATDVRL
                     RFVSLASTADPKLQGISYLYEAPAQNLLPGLSLEVTLAVASGQSGLIVPDSAVVWLEG
                     KPWIYIRTQPTIFIRKGINPTSSTPRGGFVVSGLPVDARIVIQGAQMLLSEEFRASVP
                     VED"
     gene            3698..>4493
                     /locus_tag="VGB93_02395"
     CDS             3698..>4493
                     /locus_tag="VGB93_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0145010.1"
                     /translation="MMRIAGLQASIIRFALRFRVIVVVLACLLVAYGIYVLDHASYDA
                     FPEFAPPQVDIQTEAPGFSAEQVETLVTRPIEEAVNGIGNLQRIESSSIQGLSDIKVY
                     FDVTTDRYLARQQVAERLATLANQLPAGVQAPGMTPMTSSGATIMVVGITSDRQSLMK
                     LRTIAKWTIRPSLLSVPGVAGAEVFGGEEKSTQILVHPDRLIRYGLGMEEVLAAARNA
                     TGVGGAGFISTPNQRITLQTDTSGLDSEDIARTIVPLPNGGRVTLGD"
BASE COUNT          884 a         1428 c         1339 g          842 t
ORIGIN      
        1 ccgacgctga gcctccgcca gcacccgcac cgctcaaacc gccgcggcac gcccggcata
       61 gcgcgcagca accgccgcaa gacatgcaaa acgtaccagt cgacatcagc ccgccgcccg
      121 gcttccaggc gcccccggac aatggcggcg acaatcagga caggcttggt ccgggcacgc
      181 ccaatccatc gcctgacaat ggcggtgatg acgaccaggc cccgccgccg caagtgcccg
      241 gcatgctgcc gcccgacaac aataacaata actagatttc ggacgccgga ttgccggcgc
      301 cctcaagggc tgcaaggatc tcgcgaccca tgaccggctg gtttttaggc caaaccagga
      361 aatcgccttg gtgcgcgacc gcgtctgcca gggccaggcg gtatgtctcc ttggaaactt
      421 cgatcgcccc gagcgattgc agatgcggcg tcacgaattg cgtgtcgagc aggtggaagc
      481 cgccagcggc caaccgtccg gccaaatgta tcaatgcgac tttcgacgca tcggtctgcc
      541 gatggaacat gctctcgccg aagaaggccg cgccgagcgc aacgccgtaa agtccgcctg
      601 ccaggacccc accccgccac gcctcgaccg tgtggacgcg gccgagatcg aaaagctggc
      661 ggtaaagctt cttgatccgc tgattgatcc aggtcttctc acgcccttcg ccgacgcccg
      721 cgcaggcatc gatcacggcc tcgaaatcat aatcgatccg gacctcgaag cggtttgagc
      781 gcaccagacg cgccagtttc ttggagatga tcatcttatc gagcgggaag acaccgcgcg
      841 cctctggatc gatccagaaa aggttctgat cttccgcgct ctcggccata ggaaaaaggc
      901 cgatcgaata ggcccgcatc aagatgtcgg gggtaatctc aaattcctgg cgtggccggc
      961 tcatgcctaa aagatgtgtt caggccagca gccgcgtcaa gcgccgggcg gccgggttca
     1021 ccaaaatcgt gcagcctttc gtagccgctc cgagcattgc caaaaattcc cgtataaagt
     1081 ttcatatttg gcacattcgg ccagaaatgt cgggagagag ggcctggaac atttcccgaa
     1141 attccctgag actataacaa tctaacagcc acgttcagtc gccagcagag gcgccacgcg
     1201 cggcagggaa ccgggatgac atgtgtgcgc aggctttcaa tttcaatggc agctttggcc
     1261 ggcattcttg cctctagctg cgtgcgcgat cagcccgtcc cgctttcccc cgcggtgacg
     1321 gccgcatcgc tggaaagccg tagcctcggc gatcctcgcc tgcgccaatt cgtgatcgcg
     1381 gcgacacggc cggaagctgc gcccgacgca ccaatccgct gggatttggc cagtctcacg
     1441 gcggcggcgc tctattacca tccagacatc gcgctcgccg aagccaaact ggccggggcg
     1501 aaggctgcgg tcgttacggc caacgagcat cccaatccgg tcctgaactt caccaacatc
     1561 gtcgggcaag ggctggtgcc ggaggcgatc cccatcggcg ccgcgccctt gaccatcggg
     1621 ccggcgatcg acttcatgat cgacgccttc ggccgccgcg aggcgcgcac ggcggaggcg
     1681 caacggctgg agcaggcggc gcgctgggat cttgccgcgg cgggctggca ggtgcgcagc
     1741 cgcgtccgcg ccgcactcct gacgctttgg gcaaccgagc gccgcttagc tctgacccag
     1801 caccggcttg cgctgcaaaa tcagctcgtg gaattgctgg agcatcgcca gacggccggc
     1861 gaagcgtctg cgctcgatgt ctcacgcgag cgcgtcaatc gtgccgaggt cacattggcg
     1921 atgcatgacc tcgatctggc tgtggccgag gcgcgcgttc aggtcgcaac ggccattggc
     1981 gttcccgatc aagcgctgga cggcatcacg cttgaaaccg cggttttcga ccgtccgcca
     2041 gcattggcca gagcggcaac ttcgaacgcg tggcggcgcg aagccctgac acggcgcgcc
     2101 gatattcagg cgagtctcgc gacatattcg gcgagccagt cggcgcttaa attagcgatc
     2161 gtcgggcaat atcccgatat cacactgagc cctggctaca gttacgaata cgggatcaat
     2221 caatatcagc tcaacgtcgt ctcgacgctg ccgatcttca atcagaatca gggcgcgatc
     2281 gcagaggcga ttgccaagcg gcgcgaggct gcagcgcgtt ttatcgcctt gcaggcgcag
     2341 attatcggcg agcttgatta cgcaactgtc gcctatcgta aatcaacaca atcgctgacg
     2401 agcgccgagg caatgcaggc cgatgagacg caccgcgcgc gtcaggtcga gaaatccttc
     2461 agcgcggggc aggccgatcg gccgactctc gtctcgacgc aagtggaggc cgccattacc
     2521 gcgctcacca atttcgacgc tatgctgcag caacgcgagg ctttgggggc actggaagat
     2581 gcgttggagc gaccgctcta cggtccggac atcctgctga ttttgccaga ggcgcaattg
     2641 ccgccgaggt cttcgtcatg aatgctgtca gaagatttcg cctcatggcc ttgctgttgc
     2701 tcgcttttgc ggtcggcggc gtgcttggct cgttttatct catcccctcg cacgcggatg
     2761 aggacagcga taatgacaat cccaatccag ccgtgaggct cacgatcaag gacggtgtgc
     2821 cgaccctgac gctgacggcg gaagagcagc agaatgctgg cgtcattgcc gcgaaactcg
     2881 atgtcgcgcc gccgcgggat tacatgcgcg gcttcggcac tgtgcttgat cctgccgggc
     2941 ttgccgatct cgaaaatcaa tatctcgaag ctgaaactca gattgtggca gccaaatcgc
     3001 gtctcgggag ctcgcaggct gcctatcagc gcgcgcaggt tctgaacaaa gatcaagaga
     3061 atatctcgac cgcgcaattg cagagcgcgc gcagcagctt tgatgtcgat accaatgcgc
     3121 ttgcggcagc gcaggcgcag tctgccgcca ttatcgccaa tacacgacag acctggggcg
     3181 agacgatcgc caaggccctg gcaagcggag gcccggagat caacgatctg cttgcccgca
     3241 atcattatct ggtccgcgta acgttgccgc cggacgtgac catctcgacg ccgccgtcaa
     3301 tcgcgagcgc tctctacggc gcgactgatg tgcggcttcg tttcgtgtcg ctcgcgagca
     3361 ccgccgaccc gaagttgcag gggataagct acctttatga ggcgcctgcg caaaatctac
     3421 tgccgggtct ctcgctcgaa gtcacgttgg ctgtggcgag cgggcaatcg ggcttgatcg
     3481 tgccggattc agcggtcgtc tggctcgaag gcaaaccgtg gatttacatc cgcacgcagc
     3541 cgacgatttt catccgcaag ggcatcaatc caaccagctc gacgccgcgc ggcggctttg
     3601 tcgtctcggg ccttccagta gatgcgcgga tcgttatcca aggcgcgcaa atgttgctct
     3661 cggaagaatt tcgcgcctcg gtcccggtcg aggattgatg atgcgcatcg ccggcttgca
     3721 ggcctcgatc atccgctttg cgctgcgctt tcgcgtcatc gtcgtcgtgc ttgcttgtct
     3781 gctcgtggcc tacggcattt atgtactcga ccacgcgagc tacgacgcgt ttcctgagtt
     3841 tgcgccgccg caagtcgata tccagaccga agcacccggc ttttcggcag agcaggtcga
     3901 gactctcgtc acacggccca tcgaggaagc ggtcaacggc atcggcaatc ttcaacgcat
     3961 cgagtcgagt tcgattcaag gcctttccga tatcaaggtc tatttcgatg tcacgactga
     4021 tcgttatctg gctcgccagc aggtggccga gcggcttgcg acgctcgcca atcagcttcc
     4081 ggctggcgtc caggcacctg ggatgacacc aatgacgtcg tcaggcgcca cgatcatggt
     4141 tgtcggcatc acgtccgacc ggcaatcatt gatgaagctg cgaacgattg ccaaatggac
     4201 gatccggccg agccttttat ccgtgccggg cgtcgctggc gctgaagtct tcggcggcga
     4261 ggaaaagtcg acgcaaatcc ttgtgcaccc ggaccggctg atccgttacg gcctcggtat
     4321 ggaagaagtg cttgcggcag cgcgcaacgc gaccggcgtc ggcggcgcag gctttatcag
     4381 taccccgaac cagcgcatca ccttgcagac ggatacgtcg ggtctcgatt ccgaagatat
     4441 cgcccgcacg atcgtaccgc tgcccaatgg cggccgcgtt acgctcggcg acg
//
LOCUS       DASWOG010000117         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_149202,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000117 DASWOG010000000
VERSION     DASWOG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149202"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VGB93_02400"
     CDS             complement(<1..1416)
                     /locus_tag="VGB93_02400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019564321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY0145011.1"
                     /translation="MDPMAAIKQTFFQECEEQLAELETGLLAMESGDHDPETVNAVFR
                     AVHSVKGGAGAFSLEDLVRFAHVFETALDHVRAGRLEATAELLKVLLRAADVLADLVR
                     AARDGGGVDESRSQKLADELRALNPGDVAEPDDDLSGIEFQPVLIDFDLGDEPPPPPA
                     ANVFIIYFKPKPSLYAKANETALILRELAELGEIETSCDISDIPLLSELDPEGAYLSW
                     TVRLTTTKDEDAVRDVFDFVSWDCDLEITNESEPSTGDTSLDDELAALLAHVQAPQAE
                     ISAAKAPARQIDAAPVIALPVAKHDLAEPVTPASAAAVAKSGDAENKGTTATIRVDLD
                     RVDRLIDLVGELVINQAMLAQRVTEAGLARASGIVIGLDELEQLTREIQDSVMAIRAQ
                     PVKSVFQRMPRLVRELAVMTGKAVRLVTDGENTEVDKTVIERLSDPLTHMIRNAIDHG
                     LEKPETRIAAGKPEEGTVRLSA"
     gene            complement(1434..1799)
                     /locus_tag="VGB93_02405"
     CDS             complement(1434..1799)
                     /locus_tag="VGB93_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145012.1"
                     /translation="MTKTVLTVDDSRTMRDMLLLTLSDAGYRVVQAEDGMHGLEVLAG
                     ETPDVIVTDINMPRLDGFGFIEKVRHDAKYRALPILVLTTESDAEKKDRARRAGATGW
                     IVKPFDPTKLVAAVRRVAA"
     gene            complement(1848..3686)
                     /locus_tag="VGB93_02410"
     CDS             complement(1848..3686)
                     /locus_tag="VGB93_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0145013.1"
                     /translation="MATTSIAIEVAVHHAESSIEARAASFRLVDHLDNALAAQYDLNG
                     LARGLLLSDDKAHFRGLYAAATQIFSNQIADARVEANGDPEMLATLDKFAAAGDTWHR
                     ETGDVEMQLGSDPKTVSQALALARTSAGGNAMSDVRSASDDARARAQEISAAAASAES
                     SAIWFVHLAQIIGGLLALLVAGYAGYQLDRRIAFPIQILNDCMKRLAQGRLDTGVPPM
                     GNGDEIAEMSQRIQVFKDNAVEKARLEAAAEAARQAAQEEQARQEIETQKYIDAHNVF
                     VTSITEALQRLSDGDLTFRIVKAFASEYEKIRTNFNASIEQLQQVMVHVNSNTMAIRS
                     GSQEISSAADDLSRRTEQQAASLEETAAALDEITATVRKTAEGANHAREVVAIARTDT
                     EKGGNVVRQAVNAMNAIEKSSQQISQIIGVIDEIAFQTNLLALNAGVEAARAGDAGRG
                     FAVVASEVRALAQRSAEAAKEIKGLISTSTTQVGQGVGLVTETGKALERIFTQVAEMD
                     TIVTDIATSAREQATGLQEVNTAVNQMDQVTQQNAAMVEESTAASHTLSRETEELTKL
                     VGHFQVGETEAPQKRAAKSTARPAMKTLGARGGAAPKPANDEWEEF"
     gene            complement(3970..>4338)
                     /locus_tag="VGB93_02415"
     CDS             complement(3970..>4338)
                     /locus_tag="VGB93_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0145014.1"
                     /translation="RDAKLLIVVETVADLWALERAGALSARYHVLGGTLSPLDGVGPD
                     DLNLKSLAGRVAQDEITEVVLAVNATVDGQTTAHYITDLLAPFAVRVTRLAHGVPVGG
                     ELDYLDEGTLVAAIRSRTDF"
BASE COUNT          790 a         1284 c         1345 g          919 t
ORIGIN      
        1 cgcggagaga cgtaccgtgc cttcttccgg cttgccagcc gcgatgcgcg tctccggctt
       61 ttccaaaccg tggtcgatgg cattgcggat catatgggtg agcggatcgc tcaagcgttc
      121 gatcaccgtc ttgtcgactt cagtgttttc gccatcggtg acgaggcgca ccgctttgcc
      181 ggtcatcacc gcgagttcgc gcacaagccg cggcatccgc tgaaagacgg atttcaccgg
      241 ttgcgcgcgg atcgccatca cgctgtcctg gatctcgcgc gtcaattgct cgagttcatc
      301 aaggccgatg acaatgcccg atgcgcgggc aagtccggct tcggtcacgc gctgcgccag
      361 catcgcctga ttgatgacga gttcgccaac gagatcgatc agcctgtcca cacgatcaag
      421 atcgacgcgg atcgttgccg tggtgccctt gttttccgca tcgccggatt ttgccactgc
      481 cgcggcagac gctggcgtca cgggctcggc caaatcgtgc ttcgccaccg ggagagcgat
      541 gacaggcgcc gcatcgattt gccgtgcggg tgcttttgcc gcagaaatct cggcttgcgg
      601 tgcctgaacg tgcgcgagaa gcgcggcaag ttcgtcgtcg agactggtgt cgcctgtcga
      661 aggctcggat tcgttcgtta tctcaagatc gcaatcccag ctgacgaaat cgaagacatc
      721 gcgcaccgcg tcttcatctt tcgttgtcgt gagcctgacc gtccaggaga gataggcacc
      781 ctccggatcg agttcgctga ggagcggaat gtcggaaatg tcacaagacg tttcgatctc
      841 gccgagctcg gcgagctcac gcaggatcaa ggccgtctcg ttggccttgg cgtagagact
      901 gggcttgggt ttgaagtaga tgatgaagac atttgcggcg ggtggcggcg gtggctcgtc
      961 gccgagatca aaatcgatca gcaccggttg gaattcgatg cctgacagat cgtcgtcggg
     1021 ttccgctaca tcgccaggat tgagcgcgcg taattcgtcg gcgagcttct ggctacggct
     1081 ttcatcgacg ccgccgccgt cacgggccgc gcggacgaga tcggccagca catcggcggc
     1141 ccgcaataga actttcagta gttccgccgt cgcttcaagc cggccggcgc ggacgtggtc
     1201 gagcgcggtc tcgaacacat gtgcaaatcg gaccaaatct tcgaggctga aggcgccggc
     1261 accgcccttg acggaatgga cggcccgaaa gacggcgttg acagtctctg ggtcgtgatc
     1321 gccactttcc atggcgagca ggccggtttc caattccgcg agctgttctt cacattcttg
     1381 aaagaacgtc tgtttaatcg ccgccatcgg gtccatggct cgcctccgtt aggtcaggct
     1441 gcaacgcggc gaacggcggc gacgagtttc gtcgggtcga agggcttgac gatccagccc
     1501 gtggccccgg cgcggcgggc gcgatctttc ttctctgcgt cactttccgt cgtcagaacg
     1561 agaatgggca gcgcacggta tttggcgtcg tggcggactt tctcgataaa gccgaagcca
     1621 tcgagacgcg gcatgttgat gtcggtgacg atcacatccg gcgtttcgcc ggcaagcact
     1681 tcaagcccgt gcatcccgtc ttccgcttgc accacgcggt agcctgcgtc ggaaagcgtc
     1741 agcagaagca tgtcgcgcat cgtgcgagaa tcatcgaccg tgagaaccgt tttggtcatc
     1801 gcgatattcc tttggaaatt gcttaggtag agtttgcagt agaggcctta aaattcttcc
     1861 cactcgtcat tggccggctt cggcgcggcg ccgccccggg caccgagcgt cttcatagcc
     1921 ggccgtgccg tgcttttggc ggcgcgcttc tgcggcgctt cagtctcgcc gacctggaag
     1981 tgcccgacga gcttggtcag ctcttcggtt tcgcgcgaca gcgtatggct ggcagcggtc
     2041 gattcctcga ccatggcggc gttctgctgc gtcacctgat ccatctgatt gacggcagtg
     2101 ttgacttctt gcaggccggt cgcctgttca cgcgcgcttg tcgcgatgtc ggtcacgatc
     2161 gtgtccattt cagcgacctg cgtgaaaatg cgttccagcg ctttgccggt ttccgtcacg
     2221 agaccgacgc cctgtccgac ctgtgtggtg gaagtcgaga tcagcccctt gatctccttg
     2281 gccgcttcgg cagaacgttg cgcgagggcg cgaacttccg aggcgacgac ggcgaaacca
     2341 cgacctgcat cgcccgcgcg ggcggcttcg acgccggcgt tgagcgcgag caggttggtc
     2401 tggaaagcga tctcgtcgat gacgccaatg atctggctga tctgctgcga agatttttcg
     2461 atggcattca ttgcattgac ggcctgacga acgacgttgc cgcccttttc cgtgtcagtt
     2521 ctcgcaatgg cgacaacttc gcgggcatgg ttcgcgcctt cggcggtctt gcgtaccgtt
     2581 gcagtgattt cgtcgagcgc cgccgcggtc tcttcaaggc tggcggcctg ctgctccgtg
     2641 cggcgcgaaa gatcatccgc ggccgaggaa atttcctgcg agccggaacg aatcgccatc
     2701 gtgttcgagt tgacatgcac catgacctgc tgcagctgct cgatcgaggc gttgaaattt
     2761 gtacggattt tctcgtattc gctggcgaag gccttgacga tgcggaaggt caaatcgccg
     2821 tccgaaaggc gctgcaacgc ctcggtgatc gacgtcacga agacgttgtg cgcgtcaata
     2881 tacttctgcg tctcgatctc ctggcgcgct tgctcttctt gcgcagcctg acgcgccgcc
     2941 tcggcggcag cttcaagccg ggccttctcg acagcattgt ccttgaagac ctgaatgcgt
     3001 tgcgacattt cggcgatctc atcgccatta cccatgggcg gcacgccggt atcgagccgg
     3061 ccttgcgcca acctcttcat gcaatcgttc aaaatctgga tcgggaaagc gatgcggcga
     3121 tccaactgat agccggcata cccggcgaca agcagggcaa gaaggccgcc aatgatttgc
     3181 gcgaggtgga cgaaccagat cgcgctgctt tcggcgcttg ccgccgccgc ggaaatttcc
     3241 tgggcgcggg cgcgtgcgtc gtcgctggcc gagcgaacgt cgctcatcgc gttgccgccc
     3301 gccgaggtcc gggcgagcgc aagcgcctgc gatacggtct tggggtcgct gccaagctgc
     3361 atttcgacgt cgccggtttc gcgatgccag gtgtcacctg cagcggcgaa tttatcgagc
     3421 gtggcgagca tctctggatc gccattcgct tcgacgcgcg catcggcgat ctggttactg
     3481 aatatctgcg tggcggcggc gtaaagccct cggaagtgag ccttgtcgtc ggagagaaga
     3541 agaccccgcg caagaccatt caggtcgtac tgagcggcca gggcgttgtc gagatgatcg
     3601 acgaggcgga acgaagcagc gcgtgcttcg atcgaagact cggcgtgatg aacggcgacc
     3661 tcgatggcga tggatgtggt cgccacgacg ccgagtacag ctgcgaatgc aacgataagt
     3721 tttttcgaga taggtaaatt cttcgatgcc acgccagcct ccattcagcg atcgccgcga
     3781 tcccgcggcg cggccgggga catcggcgcc attagaatgg cgaccattgt taatgagcga
     3841 gttctctgcc ccacacagcc aagatgtttc tagatgaaaa gagtgaataa attagatgca
     3901 gtcacatcgg ttgaagttac ttcgcgcgta caacttgggt gtgacgtgcg taaagcgatg
     3961 ccggcgaagt taaaaatcgg tgcggctgcg gattgccgca accaacgtgc cttcatcgag
     4021 gtaatcgagt tcgccgccga caggaacgcc gtgcgccaaa cgtgtcacgc gcaccgcaaa
     4081 cggcgccagg agatcggtga tgtaatgggc ggtggtttgc ccatcaaccg tggcattgac
     4141 ggctagaacg acctcggtga tctcatcctg cgccacgcgc ccggcgagac tttttagatt
     4201 gaggtcgtcg gggccgacgc cgtccagcgg cgacaaagtt ccgccgagca catgataacg
     4261 cgccgagagt gcgccggcac gttccagtgc ccaaagatcg gcgacagttt cgacgacaat
     4321 cagtaacttc gcatcgcg
//
LOCUS       DASWOG010000118         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_277636,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000118 DASWOG010000000
VERSION     DASWOG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277636"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGB93_02420"
     CDS             complement(<1..210)
                     /locus_tag="VGB93_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145015.1"
                     /translation="MSEIWATKYGSRRVRRDPPTLEEAMVAAQGLSDELDEQIEIAAG
                     FMDLPIDAVRTEVLKAPKNARAARVV"
     gene            complement(347..982)
                     /gene="rdgB"
                     /locus_tag="VGB93_02425"
     CDS             complement(347..982)
                     /gene="rdgB"
                     /locus_tag="VGB93_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0145016.1"
                     /translation="MSKRLSGRLVIATHNRGKLWEMQALLKPYGIEAVSAAELGLPEP
                     EETGATFAANAELKARAAADAAQLPALADDSGLCVDALGGAPSLLSARWAEIEPGGAR
                     DFANAIKKVETAVAESGAAPPYGAHFISVLALAWPHGTCDVFEGKVHGTLVFPPRGTK
                     GFGYDPIFLPDGLTRTFGEMMMDEKQAIPADGSPALSHRARAFQAFSRACL"
     gene            complement(984..1697)
                     /gene="rph"
                     /locus_tag="VGB93_02430"
     CDS             complement(984..1697)
                     /gene="rph"
                     /locus_tag="VGB93_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0145017.1"
                     /translation="MRPSQRAANEMRQVVIERNVARYAEGSCLIKFGETHVLCTASLE
                     DKPPQWLRGQGRGWVTAEYAMLPRATHTRTRRESAAGRPSGRTQEIQRLISRSLRAVT
                     DLQMLGERQITIDCDVLQADGGTRTASITGGWVALRECLKWMHSRSILKEIPLRDHVA
                     AISCGISNGEAVLDLDYAEDSAAQTDANFVITGTGALVEIQATAEAAVFSEADFGAML
                     GLARKGITELVALQKQAIA"
     gene            1897..2274
                     /locus_tag="VGB93_02435"
     CDS             1897..2274
                     /locus_tag="VGB93_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145018.1"
                     /translation="MRRFLFTSLFAATAAIGCSPMAYAQRACVPVAAAQVQSEMATQQ
                     AGGTAIALSGDQAERFLDYINDNIGQHTEYWGDGVLVTRFPALGYDAMAIVDDGCVDE
                     SKAIRLNPADTAAALQHADQDSY"
BASE COUNT          522 a          849 c          774 g          526 t
ORIGIN      
        1 gacgacgcgt gccgccctgg cgttctttgg cgccttgaga acctcagtgc gcacggcatc
       61 gatcggcaag tccatgaaac ccgccgcaat ttcgatctgc tcgtccagtt cgtcggaaag
      121 cccttgcgcc gccaccatcg cctcttcaag cgttggcggg tcacgcctta cacggcgcga
      181 accatatttt gttgcccaaa tctcagacat gtcttccgtt attccaaaaa tgcccgcgga
      241 cagctacttc gcaggccagc taggcttatt agccccgtcg ccacatggca gcaagacact
      301 ggcccgcgac atttcgtgac caatcgcaca tcatagtttt gaatgtttaa agacaggcgc
      361 gcgaaaaggc ctgaaaggcg cgggcgcgat gcgagagcgc cggcgaaccg tccgccggaa
      421 tggcttgctt ctcatccatc atcatttcgc cgaaagtccg cgtcagtcca tcggggagga
      481 atatcgggtc atagccaaag cctttcgtcc cgcgtggcgg gaagacgagc gtgccgtgca
      541 ccttgccttc aaacacatcg caagtgccat gcggccaggc gagcgccaaa acggagatga
      601 aatgcgcccc gtatggcggt gcggcgccgc tttcggcgac agccgtttcg accttcttga
      661 tcgcatttgc gaaatcgcgc gccccgcccg gctcaatctc ggcccagcgc gcggaaagca
      721 gactaggcgc gccgccaagc gcatcgacac aaagacccga atcatcggcg agcgccggaa
      781 gctgcgccgc atcggctgcc gcgcgcgcct tcaattctgc atttgccgca aaggtcgccc
      841 cggtttcctc cggctccggc aggccaagtt cggccgccga aactgcctcg atgccataag
      901 gctttagcag cgcctgcatc tcccagagct tgccgcgatt atgcgtggcg atgaccagcc
      961 ggccgctcag gcgcttcgac atttcaggcg atcgcctgct tttgcaatgc gacgagttcg
     1021 gtgatgccct tgcgggccag cccgagcatc gcgccgaaat cagcctcgct gaagaccgcc
     1081 gcctccgccg tcgcctgaat ttcgacgagc gcgccggttc cggtgatgac gaaattcgca
     1141 tcggtctgtg ccgcactgtc ctcggcataa tcgagatcga gcaccgcttc gccgttcgag
     1201 attccgcatg agatcgcggc gacatgatcg cgcaatggaa tctctttcag gatcgaacgc
     1261 gaatgcatcc atttgagaca ttcgcgcagc gccacccagc cgccggtgat cgaagcggtg
     1321 cgcgtgccgc cgtcggcctg caacacatca caatcgatcg tgatctggcg ttcgccgagc
     1381 atttgaagat cggtgacggc gcgcagactg cggctaatga gacgctggat ttcctgcgtg
     1441 cgacccgatg gccgacccgc tgcgctttcg cggcgcgtgc gtgtgtgcgt cgcgcgtggc
     1501 agcatggcat attccgccgt cacccagccg cggccctgcc cgcgcagcca ttgcggcggc
     1561 ttgtcctcaa gcgaggctgt gcaaagcaca tgcgtctcgc cgaactttat gaggcaggag
     1621 ccctcggcat agcgcgcgac attgcgctcg atgaccactt gccgcatttc attcgccgca
     1681 cgctgagaag gacgcatcaa atctttaacc tgagccattg ggaaaaacgg ccctttgctt
     1741 aggcggttcg gccaacctat gcaactgcgg ccaaattggc gtttgcaaat ggctcaagaa
     1801 ggtcgcggaa ccatcgctgc gacgccacgt ttcggcggcg ggtgatgcgg gtaccagccg
     1861 catggggcat atctgctact acgcaggaga tgcaagatga gacgttttct cttcacgagt
     1921 ctgttcgcgg ccacggctgc gattggatgc agcccaatgg cttacgcgca gcgtgcctgt
     1981 gtgcccgttg ccgcggcgca ggtgcaatcc gagatggcaa cccaacaagc cggtggcacc
     2041 gcgattgcct tatctggcga ccaagccgag cggttccttg attacatcaa cgataatatc
     2101 ggccagcaca ccgaatattg gggcgacggc gttctcgtga cccgtttccc agccctaggc
     2161 tatgatgcca tggcaatcgt cgatgacggt tgtgtcgacg aaagcaaggc tatccgcctc
     2221 aatcccgcag ataccgccgc agcgcttcag cacgcagacc aagactcgta ttgataatga
     2281 caaagcgtag ctcgggctgc acgccccggc gcgcagcccg gctctggata agccgccgat
     2341 ctgcctctag aatgagcgca gattcggcgg cgagtcgtgc ctgcatctct gcctcgccat
     2401 acactcatcc aatgccgggg cgaaaatgaa accgcatctc ttcagaagcc tgctcgctac
     2461 ggcttgtttc gcctgcctcg ttggcacatc gcaggctggc gctttgcgct gtgagccggc
     2521 tatggtggat agaatgcgcg tggccgttgc agcggcatcg gcaccgagag gcacgatttt
     2581 cttgacggac gagaaggcgg agcgattcct gacctttttg aatttcaacc ttggccagca
     2641 catggacaat tgggcgcaag ccgtcatcgt c
//
LOCUS       DASWOG010000119         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_350918,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000119 DASWOG010000000
VERSION     DASWOG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350918"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1331)
                     /locus_tag="VGB93_02440"
     CDS             complement(360..1331)
                     /locus_tag="VGB93_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY0145019.1"
                     /translation="MQRIGFVVFDGFQSMSFAALSVFEYANLMMGKPLYEVHFLSENG
                     GFVRSSTGMVVGSEPFDDTPYDTVIVGGGTSILENAPAALLGFMRKASKSSRRVASIC
                     TGAFILAEAGILDGKRATTHWWHARELQTRFPRVKMEEDRIFLIDGNVWTSAGMTAGI
                     DLALAMVEKDLGADVTRSIAQKLVIYHRRAGGQSQHSALLELAPKSDRIQNALTYAKQ
                     NLHTALSVEQLAEAAHLSPRQFSRAFRAETGQSPAKAIESLRLEAARLMMEQTRHSID
                     VVAQETGFADRRRMRQAFLRVFGQPPQTIRRNARTEALAQDPRTATR"
     gene            1469..>1594
                     /locus_tag="VGB93_02445"
     CDS             1469..>1594
                     /locus_tag="VGB93_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0145020.1"
                     /translation="MVNTQRKGTALITGSSSGIGAIYADRLARRGYDLIIVARNQE"
BASE COUNT          366 a          457 c          430 g          341 t
ORIGIN      
        1 cttcaccgtg gacgttcgcg cgccttcgaa tgctacggat tccatgtaac gccgatattg
       61 cacgatcatt acttcccgga cgatttcgag agtacatttt ccagccgcga tccatggctg
      121 cactttgtgg tcgtgatgac gtacattcga cgctcgattc gatgagctat cgaatgcgaa
      181 tgaaatacat tggtggcttc ccacgagatt ccggagaggt gttccgcggt tggcggctat
      241 agtggcggaa gaatctgatg tctcgcccgg ccgtggatca cggtgtcgca aagcgcaggc
      301 tgacatagct cgcggcaacg gcctgcgacg ccgcccgcga ttcacgcaga cagtccagct
      361 cagcgtgttg cagttcgtgg gtcttgcgct aaagcttccg tgcgggcgtt tcgccgaatg
      421 gtctggggcg gctgaccgaa aacgcgcaag aaagcctgcc gcatgcgcct acgatccgca
      481 aaacccgttt cctgtgccac cacatcaatc gaatggcgcg tctgctccat catcagtctc
      541 gccgcttcaa ggcgaagact ttcaatcgcc ttggcgggtg attgcccggt ttcagcgcgg
      601 aaggcacggc tgaattgtct tggactcaaa tgagccgcct ctgccaattg ctctactgat
      661 agagcggtgt gaagattctg cttcgcgtaa gtcaaggcgt tttgaatgcg atctgactta
      721 ggcgctagtt caagcagagc cgaatgctgg gattgcccac ccgcgcggcg gtgatagatc
      781 acgagctttt gcgcgatcga acgggtgacg tcggcgccaa ggtctttctc gaccatagcc
      841 agcgccaggt cgatgcccgc ggtcatgccg gccgatgtcc acacatttcc atcgataaga
      901 aaaatccggt cttcctccat cttcactcgt ggaaaccgcg tttgaagttc gcgcgcatgc
      961 caccaatggg tggttgcacg ctttccatcg agaatacctg cttccgcgag aatgaaagcg
     1021 cccgtgcaaa tcgacgcgac acggcgcgaa ctcttcgatg ccttgcgcat gaagccaagc
     1081 aacgcggcgg gcgcattttc cagaatggac gttccgccgc cgacaatcac ggtgtcatag
     1141 ggggtgtcgt caaaaggttc cgagccaacg accatgccgg tcgaggaacg cacaaacccg
     1201 ccgttttccg acagaaaatg cacctcataa agcggcttac ccatcatgag attcgcatac
     1261 tcgaataccg agagagcggc aaaactcatg gattgaaagc cgtcgaaaac gacgaaccct
     1321 atgcgctgca cgacaaatct cctttcaagc caggcaatgt cctgaaagag taacatatac
     1381 gtcatttcgg ccaaaagcca cctggattac atttctatcc atcagccgta cccaagtcgg
     1441 catcaacctg gaaatgtaag gaaaacaaat ggttaatacg caaaggaagg gcactgccct
     1501 tatcaccgga tcttcatcgg gcattggcgc gatctacgcg gataggctcg cgcgtcgcgg
     1561 ctacgacctt attatcgtcg cccgcaatca ggag
//
LOCUS       DASWOG010000120         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_186117,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000120 DASWOG010000000
VERSION     DASWOG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186117"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGB93_02450"
     CDS             <1..545
                     /locus_tag="VGB93_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145021.1"
                     /translation="ASAERRMADHNGGIFMPVKLSKRVPLAAALFAAAAFQCGAANAD
                     TALRSLAGYWAGSGTISMKNGTRERIRCRGSYAVSGDADALNQSLVCASDSYKFNVES
                     NVSETAGGVLRGSWAETTRNATGQLVGRVGGSHIVANVQGVGFTATISIVTRGRSQSV
                     SIAPSGGTDVVGVNVSMHRN"
     gene            complement(567..2861)
                     /locus_tag="VGB93_02455"
     CDS             complement(567..2861)
                     /locus_tag="VGB93_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0145022.1"
                     /translation="MHPLAYGIERIGLISLRFPYLVAAVLAVAAILAAFGIGRIKVDD
                     SLSQLFRSNTPEFHQYEDVTARFPSSEYDVLLVIQGDKLLARDNLSKLRDLVTDLQLI
                     DGTRGIISLFSARQPPEGDHLPAPLFPEPLPEGAAYDALLQKVMSNEILRGKLLSLDG
                     KLTLAVLALDPAVVNSGKLRDVVGEVRKTMDEDLAGSGLSAELSGVPVMQLEIRNAVE
                     RDRIVYNALGFLGGCLIAILFFRRISFMIIAAAPPLLAILLALGMLGWLDFRLNMFLN
                     VMTPLIMVISFSDSMQLTYAARDRILAGQSKADALRMAIHVVGPACVLTHATAGLSFV
                     ALTFSNSDLIRSFGEAGIIATLIALVSVLTLIPLLGMFLIRKEAAFAKSVKGADMAVD
                     ALRRFCGWIAARMVSRPGFYSLCGLIVVVGLGFIYADLQPRYHLADQVPDKEKAVQAN
                     RALDAKLTGAQPVDVLIEFPKGQSLYSPETLATIATVHSIVEKQAGVGNVWSLETLRR
                     WLAQKVGKSDVNTLRQYVSYLPKYLVERFVSKDQNAVVVEGRLPDIDSSKLLPIVDAL
                     DKNLNAVRAAHPGYEIAVTGLAVIAAHNSAGMIEKLNRGLTIEIVFVAAFIGLCFRSF
                     LVMLASIMPAVFPVFTAGAALWVLGQGLQFASVVALTVSFGLGLSATIHFLNRLRLED
                     TPGEDPAIAVERATILVGPALILTSIVLACGLAMTIFSQLPSLRLFGWLSSFAMLTAL
                     IADLFILRPTIMLFLRWSRNRLWS"
BASE COUNT          648 a          874 c         1001 g          562 t
ORIGIN      
        1 gcgcctcggc ggagcgccgc atggcggatc ataatggagg gattttcatg ccggtgaagt
       61 tgagcaaacg tgttccgctg gctgcggcgc tattcgcggc cgctgcgttt caatgcggcg
      121 cggccaatgc ggacaccgct ctgcggtcgc ttgccggtta ttgggcgggc agcggtacga
      181 tctcaatgaa gaacggaacg cgcgagcgga ttcggtgtcg tggatcttac gcggtttcgg
      241 gggatgcgga tgctttgaac cagagccttg tctgcgccag cgacagctat aagttcaacg
      301 ttgaaagcaa tgtttcggaa acggcgggtg gcgtcctgag aggttcgtgg gccgagacaa
      361 cccgcaatgc caccggccag cttgtcggtc gtgtcggcgg ctcgcatatc gtcgccaatg
      421 tgcagggcgt cggtttcacc gccacgatct cgatcgtcac gcgcggccgt tcgcaatccg
      481 tctcgatcgc tccgagcggc ggcaccgacg tcgttggtgt caatgtcagt atgcatcgga
      541 actaaacgcg gagccgccag cggggcttag ctccagagcc ggtttcggct ccagcgcaaa
      601 aacagcatga tcgtcggccg tagaatgaaa aggtcggcga tcagcgctgt cagcatcgcg
      661 aaagaactca gccagccgaa aagccgcagc gacggcaatt gcgagaagat cgtcattgcc
      721 aggccgcagg cgagcacgat cgaggtcagg atgagcgccg gaccgacgag aatcgtcgcc
      781 cgttcgacgg cgatggccgg atcttctccg ggcgtatcct cgagtcgcag acggtttagg
      841 aagtgaatcg tcgcgctgag gccaaggcca aaggatacgg ttagcgccac gacgctggcg
      901 aattgcagcc cttggcccag aacccaaagc gcggcgcccg ccgtgaagac cgggaagacc
      961 gccggcatga tggatgcgag catcaccaaa aacgagcgga agcagagccc gataaaggcc
     1021 gcgacaaaga caatttcgat cgtcaggccg cgattcagct tctcgatcat gccggcgcta
     1081 ttatgcgcgg cgatgacggc gagaccggtc acggcgatct cgtagcccgg atgcgccgcg
     1141 cgcacggcat taagattttt atccagtgca tcgacgatgg gcagcaattt gcttgagtcg
     1201 atatcgggga gccggccttc gacgactacg gcgttctggt ctttcgagac gaagcgttcg
     1261 accaaatatt ttggcaggta actgacatat tggcgcagtg tattgacgtc cgacttgccg
     1321 accttctgcg ctagccaccg ccggagcgtc tcgagcgacc agacattgcc tacgcccgcc
     1381 tgcttctcga caatcgaatg gactgtggcg atcgtcgcca gcgtttcggg cgaatagagc
     1441 gattggcctt tcgggaattc gatcaataca tcgaccggct gggcaccggt caatttggca
     1501 tcaagcgcgc gattggcctg cacggccttt tctttgtccg gcacctggtc ggcgagatga
     1561 tagcggggct gaagatccgc gtagatgaag ccaaggccga cgacgacgat gaggccgcaa
     1621 aggctgtaaa accccggccg ggacaccatc cgcgcggcta tccagccgca gaagcggcgc
     1681 agcgcatcga ccgccatatc ggcgcccttg acgcttttgg caaaggcagc ctctttgcgg
     1741 atcaggaaca tcccgagcag ggggatgagg gtcagtaccg aaacgagggc gatcagcgtt
     1801 gcgatgattc ccgcttcacc gaagcttcgg atgaggtccg aattcgagaa ggtcagcgcg
     1861 acgaaggaaa ggccggcggt cgcgtgggtc aggacgcagg ccggccccac gacatggatc
     1921 gccatccgca aggcatcggc cttgctctgg ccggccagga tccggtcgcg cgccgcgtag
     1981 gtgagctgca tcgagtcgga gaagctgatc accatgatga gcggcgtcat cacgttgagg
     2041 aacatattga gccggaagtc gagccagccg agcattccaa gcgcaagcag gattgcgagc
     2101 aacggcggtg ccgcggcgat gatcatgaag gagatgcgac ggaagaacag gatggcgatc
     2161 aggcacccgc cgagaaagcc aagtgcatta tagacaatcc ggtcgcgttc gacggcgttg
     2221 cggatttcga gctgcatcac cggcacgccg gacagctccg cggaaaggcc ggacccggca
     2281 aggtcctcgt ccatcgtctt gcggacttcg ccaacgacat cacgtaattt gccgctgttg
     2341 acgacggctg gatcgagcgc cagcacggcc agggtcagct tgccgtcaag cgaaagcagc
     2401 ttgccgcgca ggatttcgtt gctcatcacc ttttgaagga gcgcatcata ggctgcgccc
     2461 tcaggcaaag gctcggggaa cagcggcgcc ggcaggtgat cgccttccgg cggctggcgg
     2521 gcggagaaca gggaaatgat tccgcgcgtt ccgtcgatca attgcaggtc ggtgacgagg
     2581 tcacgaagct tgctcagatt gtcgcgcgcc agcagcttgt cgccctggat gacgaggagg
     2641 acgtcatact cactcgacgg gaagcgcgcc gttacatcct catattgatg aaactcgggc
     2701 gtgttcgagc gaaacaactg gctgagtgaa tcgtctacct tgatacggcc aatgccaaag
     2761 gcagcgagaa tcgccgcgac cgcgagcaca gcggcaacga gataggggaa gcgcaacgag
     2821 ataagtccga tgcgctcgat cccgtacgcc agcggatgca ggttacggcc ttcgccagtc
     2881 gccgggggct gtttaaggag tgtttccggg tcttcgctcg acaggatttc tctccgcggc
     2941 aatcagcgac gcgcatcttg gcgcgaaggc gtgtcagctt ctccacaccc tgtagtggcg
     3001 ggtgcgattg tggcctaggt gtgaagatcg agcaccgcct tgtaaaggcc ttgggtcttt
     3061 tttagtggga cgtaaagtac ggata
//
LOCUS       DASWOG010000121         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_2904, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000121 DASWOG010000000
VERSION     DASWOG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2904"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VGB93_02460"
     CDS             complement(<1..1487)
                     /locus_tag="VGB93_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY0145023.1"
                     /translation="MLALWIIIAAGVLAVAYGIWTANELLKADAGSPRMQEIAAAIAE
                     GAQAYLKRQYSTIAIVGIVIFVVLGFLLSWTVAIGFLIGAILSGAAGFIGMNVSVRAN
                     VRTAQAASKSLSAGLDISFKAGAVTGLLVAGLALLGVSSYFWILTGPLKLAPDAREVV
                     DALVALSFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPATIADNVG
                     DNVGDCAGMAADLFETYVVTVVATMVLAAIFFAGTPQLVPTMFYPLAICAACIVTSIA
                     GTFFVKLGPTNSIMGALYKGLIATGLLSIVGLAIATQFSVGWGTIGVVHGQTVSGLHL
                     FVCGLLGLVVTGLIVVITEYYTGTGKRPVISIAQASVTGHGTNVIQGLAVSLEATALP
                     ALAIVAGIIIASQLAGLFGIAIAVTTMLGLAGMIVALDAFGPVTDNAGGIAEMAGLPK
                     EVRHSTDALDAVGNTTKAITKGYAIGSAGLGALVLFAAYTNDIKH"
BASE COUNT          407 a          555 c          536 g          294 t
ORIGIN      
        1 aaatgcttga tgtcattggt ataggccgcg aagagcacga gcgcgccgag acccgccgag
       61 ccgatcgcat aacctttggt gatcgccttg gtcgtgttgc cgaccgcgtc gagcgcatcg
      121 gtcgaatgac gcacttcttt cggcaagccg gccatttcgg cgatgccgcc ggcgttatcg
      181 gtcacgggac cgaaagcgtc gagcgcgacg atcatgccgg caaggccaag catcgtcgtc
      241 acggcaatgg cgatgccgaa gagtccggcc agttgcgagg cgatgatgat accggcaacg
      301 attgccaaag ccgggagcgc cgtcgcttca agcgaaacgg caagcccctg aatgacattg
      361 gtaccgtggc ccgtcacgga cgcctgcgcg atcgagatca ccggccgttt gccggtgccc
      421 gtgtaatatt cggtgatcac gacgatgagc ccggtcacaa ccaggccaag tagcccgcag
      481 acgaagaggt gcagtccgct gaccgtctgg ccatgaacaa cgccgatggt gccccagccg
      541 actgagaatt gcgtcgcgat cgcaagcccg acgatcgaca gcaatccagt ggcaatcagg
      601 cccttgtaga gcgcgcccat gatcgaattg gtcgggccga gtttgacgaa gaacgtaccc
      661 gcaatcgagg tcacgatgca ggccgcgcag atcgcgagcg gataaaacat cgtcggcacg
      721 agctgcggcg ttccggcgaa gaagatcgcg gcgagcacca tggttgcaac gaccgtcacg
      781 acataggtct cgaaaaggtc agcggccatg ccagcgcaat cgccgacgtt atcgccgacg
      841 ttgtccgcga tcgtcgccgg attgcgcgga tcatcctctg gaatccccgc ctcgaccttg
      901 ccgacgagat cggccccgac gtcagcgccc ttggtgaaaa taccgccgcc aagacgcgcg
      961 aaaatggaga tgagcgaggc gccgaaactg agcgccacaa gcgcatcgac aacttcacgg
     1021 gcgtccggcg cgagcttcaa cggcccggtc aaaatccaga aataggacga caccccgagg
     1081 agcgcgagac cagccacgag aagaccggta acggcgcccg ccttaaacga aatatcgagc
     1141 cccgcgctga gcgatttcga tgcagcctgc gccgtgcgga cgttggcgcg cacggaaaca
     1201 ttcataccga tgaagccggc agcgccggag aggatcgcgc cgatcaggaa gccgattgcg
     1261 accgtccacg acagcaggaa gccgagcacg acaaagatca ctattccgac aatcgcgatg
     1321 gttgaatatt gccgcttgag ataggcctgc gcgccttcgg cgatcgccgc cgcgatctcc
     1381 tgcattcgcg gcgaaccggc gtccgccttc aaaagctcat ttgccgtcca gattccatat
     1441 gcgacggcca aaacccccgc agcgataata atccaaagtg caagcatcga tttccgccct
     1501 tcgtttatga ttgcgctgcc ggatttttac ccggcttata tttcgcgcgt ttgatcggag
     1561 gtcattgcaa aaaggcaaag ccgcgggacg gccgcccatt cctcatcgcc cccaccgagc
     1621 ggcgcaccgc ccagcgcgca gcttgatcga aagcgcggcg tttcgcaatc ctacgagcgg
     1681 acgaaaggag cagacttaaa agtgggcgta ggacaatgac ttgagcgcta gtgcttggcc
     1741 cgcattgtcg cgaaaaacag gcggatttgt gcccgctgcc acgatgccaa tg
//
LOCUS       DASWOG010000122         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_222793,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000122 DASWOG010000000
VERSION     DASWOG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222793"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..981)
                     /gene="fmt"
                     /locus_tag="VGB93_02465"
     CDS             complement(49..981)
                     /gene="fmt"
                     /locus_tag="VGB93_02465"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0145024.1"
                     /translation="MRVIFMGTPDFAVPVLTEIAAHGHDIAAVYTRAPKPAGRRGLET
                     LPSPVAVAAAKLGLAVYTPVSLRTAEAASLFACHKADVAVVVAYGLILPKAILDAPAK
                     GCLNLHASLLPRWRGAAPIQRAIMAGDRKTGVAVMCMDETLDTGPVALTECVPIGPEM
                     TAGELHDELSLLGAALMARALDLLARDALTFALQSETGIIYAEKISKAEALIDWSRDA
                     DEVHNLVRGLSPFPGAAFLANFGKGAERIKVLRSRIIEAQGPPGTILDDELTIACGRD
                     ALRLLEVQRAGKAPTLAADFLRGARLTRGTRLNA"
     gene            complement(986..1558)
                     /gene="def"
                     /locus_tag="VGB93_02470"
     CDS             complement(986..1558)
                     /gene="def"
                     /locus_tag="VGB93_02470"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0145025.1"
                     /translation="MALRPILILPDKRLRQKAQPVAAVDDEIRALMDDMLETMYAAPG
                     IGLAATQIGEMKRILVLDVTKRQEEEASAHPMAFANPEVLWASDELSTYNEGCLSIPE
                     IYEEVERPARIRVGYLDPDNKRQEIEADGILATCLQHEIDHLNGVLFIDHISRLKREM
                     ISKKFIKAAKRDAEDAPSEPEAETKRQSEG"
     gene            complement(1703..>2292)
                     /locus_tag="VGB93_02475"
     CDS             complement(1703..>2292)
                     /locus_tag="VGB93_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0145026.1"
                     /translation="QVARDAADKADETQDAVDSLSEAAERIGGSVVLISNIAAQTNML
                     ALNATIEAARAGEAGRGFAIVAQEVKNLALATSRATSEIATQVAGILAATPHVASFIA
                     SIAKTTQQVSSIAIAAETAVAEQEAATREIARRAEQASLGTHETTANIIGVTDAAGNA
                     NAAAKQVLDLATRLTHQSDLLAKQVRDFLTMVQAA"
BASE COUNT          394 a          708 c          738 g          452 t
ORIGIN      
        1 ccacaaggcc ctggccgtca tattctatcg ttagcttata gcgtggcatc atgcgttgag
       61 ccgcgtgccg cgcgtcagtc gcgcgccgcg cagaaagtcg gccgccagcg tcggcgcttt
      121 gccagcgcgc tgcacttcca aaagccgcag cgcgtcgcgg ccgcaagcaa tggtcagctc
      181 gtcgtcgagg attgtgcctg gggggccttg ggcctcgatg atccggctgc gcagcacctt
      241 gatccgctcg gcgcccttgc cgaaattggc aagaaaagcc gcgccgggaa agggcgacag
      301 accacgcacc agattgtgca cttcgtcggc gtcacgcgac cagtcgatca gcgcctcggc
      361 cttgctgatt ttctccgcat agatgattcc ggtctcggac tggagcgcaa aggtcagggc
      421 gtcccgcgcg aggagatcga gcgcgcgggc catgagggcc gctccaagca aagacagttc
      481 gtcgtgcagc tcgccggccg tcatctctgg cccgatcggg acgcattcgg tgagcgcgac
      541 aggcccggta tcgagcgtct catccatgca catgaccgcg acgcccgttt tcctgtcgcc
      601 agccatgatc gcccgctgga tcggcgcggc gccgcgccag cggggcagaa gcgaggcgtg
      661 cagattgagg catcccttcg ccggcgcatc gaggatggct ttcggcaaga tgagcccata
      721 ggcgacgacg accgccacgt cggctttgtg gcaggcaaag agcgatgcgg cttccgccgt
      781 gcgcaggctg acgggcgtgt agacagcaag gccgagtttc gccgccgcaa cggcaacggg
      841 cgagggaagg gtttccaggc cgcgccgccc ggcaggcttg ggcgcgcgcg tgtagaccgc
      901 cgcaatgtcg tgtccgtgcg cggcaatttc ggtcagcacc gggacggcaa aatccggcgt
      961 gcccatgaaa atgacgcgca cgcgctcagc cttccgattg acgtttggtc tcggcttccg
     1021 gctcgctcgg tgcatcctcg gcgtcgcgct tggccgcctt gatgaatttc ttcgagatca
     1081 tctcgcgctt tagccgcgag atgtggtcga tgaagagcac accgttcaga tgatcgatct
     1141 cgtgctgcag gcacgtcgca agaatgccgt ccgcctcgat ctcctgtcgc ttgttatcgg
     1201 ggtcaaggta gccgacgcga atccgcgccg ggcgctcgac ttcctcgtaa atctcgggga
     1261 tcgacagaca gccctcatta tacgtggaga gttcgtcgga cgcccaaagc acttccggat
     1321 ttgcgaaagc catggggtgg gcgctcgcct cttcctcctg ccgtttggtg acatctagaa
     1381 cgaggatacg cttcatctcg ccaatctggg ttgcggcgag gccgatgcct ggcgccgcgt
     1441 acatcgtctc cagcatgtcg tccatgagcg cgcggatctc gtcatcgacg gcggcgacgg
     1501 gctgagcttt ctgacgaagc cttttgtctg gcaggatgag gatcggtctt agggccatag
     1561 cggagaaaat tgagcggact gaacgagctt ggcctcaaga cataggcgtc acgccgcgcc
     1621 gggtcaattt caggctgttc aaagtgtttt tgagcgaagt ggggccggtt cgcgtgaaga
     1681 aaacgcgtca aagtaaaaaa ctctaggcgg cctgcaccat cgtcaaaaag tcacgcactt
     1741 gtttggccag aaggtccgac tggtgcgtga ggcgcgtggc aagatccaat acttgcttgg
     1801 ccgcggcgtt ggcattgccc gcggcgtcgg tcacgccgat gatattcgcg gttgtctcgt
     1861 gggtgccgag cgaggcctgt tcagcgcgcc gcgcgatctc gcgtgttgcg gcttcctgct
     1921 ccgccaccgc ggtttccgcg gcgatggcaa tggaacttac ctgctgcgtc gtcttcgcga
     1981 tgctggcgat gaacgaggcg acatgtgggg tagcggccag gatgcccgcg acttgtgtag
     2041 ctatttcaga ggtcgcccgc gaggtcgcga gtgcaagatt tttgacttcc tgcgcgacaa
     2101 tggcaaagcc acgtcccgct tcgcccgcgc gcgcggcttc gatcgtcgcg ttgagcgcca
     2161 gcatgttggt ctgcgccgcg atattgctga tcagcaccac gctgccgccg atccgctcgg
     2221 cggcttcgga aagactatcc accgcatcct gcgtctcatc agccttgtcg gctgcatccc
     2281 gcgccacctg at
//
LOCUS       DASWOG010000123        10524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_222890,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000123 DASWOG010000000
VERSION     DASWOG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222890"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGB93_02480"
     CDS             <1..338
                     /locus_tag="VGB93_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145027.1"
                     /translation="REIRRRLDQITFNAALQTEVETLERQIDRSQKLAGLLSSESRKL
                     RRLAIHQISAEEAFDGLADANATNLDWNFLTTGRDHGRAAAAAWLAGKKSNNIVPHAA
                     APLEASYAP"
     gene            387..1859
                     /locus_tag="VGB93_02485"
     CDS             387..1859
                     /locus_tag="VGB93_02485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0145028.1"
                     /translation="MKPAASAAVAKDAMAVDVSLFIDGAWRPGASGRTLDVINPATED
                     VIGTVARAEIADLDAALATAQKGFAVWRAISAFERGKILRRAAVLLRERSEKIAHVMT
                     REQGKPVKEAKVEISVAADTLDWFAEETRRAYGRVVPARAPKITNLVLKEPVGPVAAF
                     TPWNFPINQIARKLGAALAAGCSIIVKAPEEAPGSPAEFIRALADAGVPAGVINLVFG
                     VPAEISAYLIPHPLIRKISFTGSTAVGKELAALAGAHMKRATMELGGHAPAIVFDDAD
                     IDVALRVLVARKFRNAGQACTAPTRFLVQRDVYQNFLTRFVAATKALKIGDGLDSATD
                     VGPLANPRRVAALESFVADAVKKGAKLVIGGTRQGHKGYFFAPTVLAHVPTNARAMNE
                     EPFGPIALVNSFETFDEAIAEANRLPYGLASYAFTSSAATADALAQRIEAGMVAINDS
                     HLSSPELPIGGVKDSGYGSEGGPEAMDAYLVTKLVTQAAG"
     gene            2089..2460
                     /locus_tag="VGB93_02490"
     CDS             2089..2460
                     /locus_tag="VGB93_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145029.1"
                     /translation="MNVEGIVSSDITPRVLSRMKRIGIDISKRAALKDKPSMIIDGVS
                     YSRVYKTSIPKIPTVYPTVFSYSVEFAGDCPAIVRIEADSDDGATYFIGCGGLAGDKE
                     VNTVKTLGNTIKVRMTLYPTF"
     gene            complement(2457..3239)
                     /locus_tag="VGB93_02495"
     CDS             complement(2457..3239)
                     /locus_tag="VGB93_02495"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF003986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEY0145030.1"
                     /translation="MRRVAFVAGAILLLVMGAGAFWWLHRINHRDDLWHHVYDECVPS
                     QQAHGDPAPCAFVALGQGAARGFAIYKDQIGKTQFLLIPTRKISGIDDPSLLLPGAPN
                     YWASAWQARAYAEKAAGRAIPWDDIGLAINSAVTRSQDQLHIHIDCVQPHVHAALVKY
                     IGSVGAIWAKFSVPLHGMPYLARRLAATDLAAEDPFKLLATGVPGAAQDMRDQTLALI
                     GADFGGGEKGFVLLAGRADLAKLRFAHAANLLDHSCKLLGNN"
     gene            complement(3243..3905)
                     /locus_tag="VGB93_02500"
     CDS             complement(3243..3905)
                     /locus_tag="VGB93_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145031.1"
                     /translation="MKVKASIFAALGLVALGKPAQASEAWPDTFRGLLEAWALMETLN
                     ATLLASNSATKTLESWCGTHHMAADAVLHARLRRDVQKPITAEQRKRLNIGPNETVVY
                     RRVDLVCGDHVLSQADNWYVPSRLQPAMEKALETSDIPFGRAILPLHPRRQTIGVDLL
                     WRPLPDGWEVNPPADHSRANLAIPPVLFEHHAIVFAADGQPVSEVEEHYTSAVLDFRH
                     GE"
     gene            complement(3915..5138)
                     /locus_tag="VGB93_02505"
     CDS             complement(3915..5138)
                     /locus_tag="VGB93_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145032.1"
                     /translation="MSSITSDTAGGLLRHRPFLFYFLGRGFTESASQIAAVAVGWQVY
                     TLTGSPLALGLIGLAQFVPVLLLTLVAGHAADRYDRRRVLQICQFCAGLVAIFLAVGS
                     FAGWLTVPEIFAAIVLVGIFDAFESPVKGALLPAVTPEGALQKGAALASGAMQIASIG
                     APALGGFIYAIAPGAPYSVAAVLWLLATLANGAIKLERPIMTRASAPLEGLFDGVKFV
                     RDNPAILGTISLDLFTVLFGGATALLPVYAHDILHTGPLGLGMLRAAPAIGALLMAGF
                     LTRHPLRRRVGMRMLQAVIGFGLATIVFALSHSMWLSMVALAVAGAADTVSVVIRVSL
                     VQLATPDAMRGRVGAVNYLFIGTSNQLGEFESGTLAALIGAMPAVLLGGIASIGIALL
                     WMRLFPTLRDVETLE"
     gene            complement(5195..5830)
                     /locus_tag="VGB93_02510"
     CDS             complement(5195..5830)
                     /locus_tag="VGB93_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0145033.1"
                     /translation="MPAAVADDTPPPKPQNILSPDEALARLKAGNKRYVEGVAKRHDF
                     VAEREALVGGQNPFAGILSCADSRIAPEYAFDTSRGDLFVVRVAGNFVNTDNLASFEY
                     AVAELKTPLILVLGHEACGAVKATISSIKTETILPGHLPSLVKALAPAVTAVLSKGGD
                     VLENAIEENVRRNVAALKDASPILSAAVEKKRVAIVGGIYRLASGEVEIIA"
     gene            complement(6011..6985)
                     /locus_tag="VGB93_02515"
     CDS             complement(6011..6985)
                     /locus_tag="VGB93_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0145034.1"
                     /translation="MAAVRTIAILAPPGVQLLDVSGPLDVFAEANVQTGKIVYRPLVV
                     GTEAAPIRSSSGVRLMPDAVMGALHDEKIHTLLVAGCPDIADTAADPAMVAWLRDVTA
                     SARRYGSVCSGAFLLAAAGLLDGCRVTTHWAVAGRLAERYPSVIVDEDAIYLRDGRLR
                     TAAGVTAGLDLALALVEEDMGRDITMKVASQLVMFFKRPGGQRQFSRKGEAAPAGRSA
                     LQELQRWVIANPAKTHNVAALAKRADLSPRHFARLFRSEVGATPAAWVESIRVDAVRQ
                     MLESGRVAPKQVAVKCGFADADTLRRAFSRHVGVTPAEYRKRYARPEN"
     gene            7097..7738
                     /locus_tag="VGB93_02520"
     CDS             7097..7738
                     /locus_tag="VGB93_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0145035.1"
                     /translation="MTLDISGVRIPDSKLAREVTELVRGTAWPLLFHHSSRVYYFGAL
                     AGKHRGLSFDAELLYTGAMFHDTGLTAQYSSDHDRFEVDGANAARDFLKRHGIAQSDI
                     DTVWTAIALHTTPGIPQYMHPVVALVTAGVEMDVLGLTYPEYSDVEREAVVHAHPRTP
                     HFKEDIIQAFYDGIKHKPETTFGNVKADVLADKDPHFHRGNFCSVIRNSPWKG"
     gene            complement(7754..8851)
                     /gene="ychF"
                     /locus_tag="VGB93_02525"
     CDS             complement(7754..8851)
                     /gene="ychF"
                     /locus_tag="VGB93_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0145036.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGD
                     VAVPDPRLEVLAKVAGSKEIIPTRLTFVDIAGLVRGASKGEGLGNQFLANIRECDAIA
                     HVVRCFEDDDITHVAGKISPLDDIETIETELMLADLDSLEKRAPALEKKAKGGDKEAK
                     ETFDIVSRCLVLLREGKPARLAAIKPEEQKAFIMLCLLSAKPVLYVCNVEEASADKGN
                     AYSEQVKVRAAQEGAGAVIVSAAIESEIALLPAAEQKDYLEAVGLEEPGLNRVIRAGY
                     ALLHLITYFTVGPKETRAWTIEVGTKAPQAAAVIHTDFEKGFIRAETIAYADYVAANG
                     EAGAREAGKFRLEGKDYVVADGDVLHFRFAN"
     gene            complement(8917..>10524)
                     /locus_tag="VGB93_02530"
     CDS             complement(8917..>10524)
                     /locus_tag="VGB93_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0145037.1"
                     /translation="NSYFGAMIGVVGVLSLASGARYYLVNTLGERVVADLRSAVFRHL
                     SGLDASFFDSARIGELLSRLTADTTQMKAAFGASASVALRNFFMFIGAVALMVYSSPR
                     LSAYVLVAIPIIVLPLYAAGRAVRKRSRAAQDTLADASAYAAENLGAVRVMQAFGAEA
                     TTNARFTAAVENSYDAARLSTLARGIVTMIAIFLAFASVVVVLWLGAHDVLAGRMTGG
                     LLSQFVLYAVLGAGALGELSQVWNEISAAAGAAGRIGEILAIKPKITAPLPAAQLPLP
                     GKGEIVFDHVNFAYPTRQDAPALHDLSFRVAPGETVAIVGPSGAGKSTIFQLLLRFYD
                     PTDGEIDLDGVDIKTVDPAELRRRISLVPQEPVIFGSSFADNIRYGTPAASEAAVEAA
                     AKKAAAQDFIVATSQGYATNIGERGVTLSGGERQRLAIARAILREAPVLLLDEATSAL
                     DAASEGLVQHALEELKQGRTTLVIAHRLATVLSADRILVLEAGRIVEEGTHQSLVAKD
                     GLYAKLAKMQFETGALALNGSRSAAAE"
BASE COUNT         2182 a         3243 c         3204 g         1895 t
ORIGIN      
        1 cgcgcgaaat ccggcgccgg cttgatcaga tcacgttcaa cgccgcgctg cagaccgagg
       61 tggaaacgct ggagcggcaa atcgaccgca gccagaaact cgccggcctg ctgtcgagcg
      121 aaagccgcaa attgcgccgc cttgcaatcc atcagatctc ggcggaggag gccttcgacg
      181 gccttgccga cgcaaacgcg accaatctcg attggaactt cctgacgacg gggcgcgatc
      241 atggccgcgc tgcggcggcc gcgtggctcg ccggcaagaa atccaacaac atcgtgccgc
      301 acgccgccgc gccgctcgag gcctcctacg cgccgtgacg atttagacca agcgaaacgc
      361 catcgcccgc actatatgaa gaacgcatga aaccggcggc ctctgccgcc gtggcgaaag
      421 acgcgatggc tgtggacgtt tctctcttca tcgacggcgc atggcgcccc ggcgcgagcg
      481 gccgaacgct cgatgtgatc aatccggcaa cggaagacgt gatcggcacg gtcgcgcgtg
      541 ccgagatcgc cgacctcgac gcggcgctgg cgacggccca aaaaggcttt gccgtctggc
      601 gcgccatatc ggctttcgag cgcgggaaaa tcctgcgccg cgccgcggtg ctcttgcgcg
      661 agcggagcga aaagatcgcg cacgtcatga cgcgcgaaca aggcaagccg gtcaaggaag
      721 ccaaagtcga aatcagcgtc gccgccgata ctctcgattg gtttgccgaa gagacgcggc
      781 gcgcttatgg ccgcgtcgtc ccggcgcgtg cgccaaagat caccaatctg gttctgaaag
      841 agccggtcgg gccggtcgca gcgttcacgc cgtggaattt tccgatcaac cagatcgcgc
      901 gcaagctcgg cgccgcgctg gcggcgggtt gttcgatcat cgtcaaagcg ccggaagaag
      961 cgccgggttc cccggctgaa ttcattcgcg ccctcgccga tgccggcgtg ccggctggcg
     1021 tcatcaatct cgtcttcggc gtaccggcgg aaatttcggc gtatctcatt ccgcatcctt
     1081 tgatccgcaa gatttccttc actggctcga cggccgtcgg caaagaactg gcagccctcg
     1141 ccggcgcgca tatgaaacgc gcgacgatgg aactcggcgg tcacgcgccc gcaattgttt
     1201 tcgacgatgc cgacatcgat gtcgcgttgc gcgttctcgt ggcgcgtaag ttccggaatg
     1261 ccgggcaggc ctgcacggcg ccaacgcgct tccttgtgca gcgtgacgtc taccaaaatt
     1321 ttctgacgcg tttcgtggcg gcgaccaagg cgctgaaaat cggcgatggt ctcgattccg
     1381 cgaccgacgt cggcccgctg gccaatccgc ggcgcgttgc ggccctcgaa agcttcgttg
     1441 ccgatgcggt taagaaaggc gccaaactcg tcatcggggg tacgcgccaa ggccacaagg
     1501 gctatttctt cgcgccgacg gttcttgcgc atgtgcccac gaatgcgcgg gcgatgaacg
     1561 aagagccgtt cggtccgatc gcgctcgtta attcgttcga gactttcgac gaagcgattg
     1621 ccgaagcaaa ccgacttccc tacggcctcg cctcctatgc cttcacctca tcggcagcga
     1681 cggccgatgc gctcgcgcaa aggatcgagg cggggatggt cgcgatcaac gattcgcatt
     1741 tgagttcgcc cgaactgccc atcggcggcg tcaaggacag cggctacggc agcgagggcg
     1801 gccccgaggc gatggacgcc tatctcgtca ccaagcttgt gacgcaggcg gcgggttaag
     1861 ccccggcatt gcgggtgaag cgaagcaatc cagaatgaag actggatttc ttcgtcgcca
     1921 acgctcctcg caaagagact gcgccacaca atttcgacta actttttgat cttctaatca
     1981 gcatagctga ttatctggac atttattgcg atgaagaaac ttcctgtaat ctcagccttc
     2041 gttctgtcgt cgctcgtata tttgcccgca atttgtcacg cttgcgcgtt gaacgtcgaa
     2101 ggaattgttt cgagcgacat aacgccccgc gtactcagca gaatgaagcg tataggcatc
     2161 gatatttcaa aacgtgccgc gctaaaggac aagccttcga tgatcatcga cggtgtgtcg
     2221 tatagtcgtg tctataagac ttcaatcccg aaaattccga ccgtatatcc gacagtattt
     2281 tcatattcag tggaatttgc aggtgattgc ccggcaatcg ttcgtatcga agctgatagc
     2341 gacgacggcg cgacttattt catcggttgc ggagggcttg ccggtgacaa ggaggtaaat
     2401 acggtaaaaa cgcttggaaa cactatcaaa gtccgcatga ccctgtatcc aacattctaa
     2461 ttattgccca gcagcttgca cgaatggtcg agcagattgg cggcatgcgc gaaacgcagc
     2521 ttggcgaggt ctgcacgccc ggcgagaagt acgaagccct tttcgccgcc accaaaatcg
     2581 gcaccaatca gcgccaatgt ctggtcgcgc atgtcctgcg ctgcgcccgg cacgccggtc
     2641 gcaagcagct tgaacgggtc ttccgccgcc agatccgtcg cggcgagccg gcgggcaaga
     2701 taaggcatac cgtgcagcgg caccgaaaat ttcgcccaga tggcgccgac actgccgatg
     2761 tatttgacga gcgccgcatg cacatggggc tgaacgcaat cgatatggat gtgcaattga
     2821 tcctgactgc gcgtgaccgc tgaattgatg gcgagcccga tgtcatccca gggaattgcg
     2881 cgccctgccg ctttctcggc ataggcgcgc gcttgccagg ccgacgccca ataattcggt
     2941 gcgcctggca ataagagact cggatcgtcg atgccggaaa tcttgcgcgt cgggatcagc
     3001 aaaaattgcg tcttgccgat ttgatccttg tagatggcga aaccgcgcgc ggcgccctga
     3061 ccgagcgcga cgaacgcgca aggcgcggga tcgccgtgtg cctgttggga cggcacacat
     3121 tcgtcataga catggtgcca gagatcgtcg cgatgattga tccggtgcag ccaccagaat
     3181 gcgccggcgc ccatcacaag gagcaggata gctccggcga cgaaggccac acgccgcatc
     3241 gatcactcgc cgtggcggaa atcgagaacg gcgctggtgt aatgctcctc gacttctgaa
     3301 accggctggc cgtcggcggc gaacacaatc gcgtgatgct cgaacaaaac cggtgggatc
     3361 gccagattgg ccctcgaatg gtcggccggc ggattgacct cccaaccgtc cggcaacgga
     3421 cgccacaaaa gatcgacgcc gatggtctgg cggcgcggat gcagcggcag aatggcgcgt
     3481 ccgaagggaa tgtcgctggt ttccagcgcc ttttccatcg ccggctgcag gcggcttggc
     3541 acataccaat tatcggcctg cgacaggacg tgatcgccgc agacgagatc gacccggcga
     3601 tagaccacgg tctcattggg accgatattg aggcgcttgc gctgctcggc cgtgatgggc
     3661 ttttgaacat cgcgacgcag ccgggcatgg agtacggcat cggcggccat gtggtgcgtg
     3721 ccgcaccagg attcgagcgt tttggttgcg ctgttcgagg cgagtaaggt cgcgttcagc
     3781 gtttccatca aggcccaggc ttcgagcaga ccgcggaacg tgtcaggcca cgcctccgac
     3841 gcttgcgccg gtttgcccag ggcgacaaga cccagagcgg caaaaatcga agccttcacc
     3901 ttcatggtcg acaattattc caatgtctcg acatcacgca aggtggggaa tagccgcatc
     3961 cagagcaagg caatgccgat cgaggcgatt ccgcccagca ggaccgccgg catcgcccca
     4021 atgagcgccg ccaaggtgcc gctctcgaat tcgcccaatt gattcgaggt tccgatgaaa
     4081 aggtagttaa ccgcgccgac acggccgcgc atcgcatccg gcgttgcgag ctggacgagc
     4141 gacacgcgga tcacaacgct caccgtatcg gccgcgccgg ccaccgcgag cgcaaccatg
     4201 gaaagccaca tcgaatgtga tagcgcgaag acgatcgttg ccagaccgaa gccgatgacc
     4261 gcctggagca tccgcatccc gacgcgccgt ctcagaggat gccgtgtcag aaagccggcc
     4321 ataagcagcg cgccgatcgc cggggccgca cgcagcatgc caagccctag cggccccgtg
     4381 tgcaaaatat cgtgcgcata gaccggcaag agcgccgtgg cgccgccgaa cagaacggtg
     4441 aaaagatcga gcgaaatcgt gccgaggatc gcgggattgt cacggacgaa cttcacgcca
     4501 tcgaagagac cttcgagcgg tgctgacgcc ctcgtcatga taggccgctc aagcttgatc
     4561 gcgccgttcg caagcgtcgc caaaagccaa agcaccgctg caacagaata gggcgcgccg
     4621 ggcgctatcg catagatgaa gccgcccagc gcgggtgcgc cgatcgaggc aatctgcatc
     4681 gcgccgctcg ccagcgccgc gcctttctgc agcgcgccct cgggcgtaac cgccggcaag
     4741 agcgcgccct ttaccggact ctcgaacgcg tcgaaaatgc cgacaagcac gatggcggca
     4801 aaaatctccg gcactgtgag ccagccggcg aagctgccga cagcgagaaa gatggcaacg
     4861 agccccgcgc aaaactggca gatctgaagc acgcgccgcc ggtcgtagcg gtcggcggcg
     4921 tggccagcaa cgagcgtcaa caaaagcacc ggtacgaatt gcgcgagtcc gataaggccg
     4981 agagcaagcg ggctgccggt cagtgtgtag acctgccacc cgacagcgac agcggcgatc
     5041 tgagaggcgg attccgtgaa accccgaccg agaaaataaa acagaaaggg ccggtggcgc
     5101 agcaggccgc cagccgtatc ggatgttatc gaactcattg aaccgttttg cgtggaagaa
     5161 tggcagccaa cctacggaag ccacgcccga aagatcaagc gataatctcg acctcgccac
     5221 tcgccagccg gtagataccg ccgactatgg cgacgcgttt tttttcgacg gcggcgctga
     5281 ggatcggcga cgcgtctttc agcgcggcga cattgcggcg aacattttct tcgatggcgt
     5341 tttcgagtac gtcgccgccc ttcgaaagaa cggcggtcac cgccggcgcg agggccttga
     5401 cgagactcgg caaatgcccc ggcaaaattg tctcggtctt gatcgacgaa atggtcgcct
     5461 tcaccgcgcc gcacgcttcg tggccgagca cgaggatcaa cggtgttttc aactccgcca
     5521 cggcatattc gaaactcgcg agattgtcgg tattgacgaa attgcccgcg acacgaacga
     5581 cgaagagatc accgcgcgat gtgtcgaacg catattccgg tgcaatacgt gaatcggcgc
     5641 aactgagaat gccggcaaag ggattctggc ccccgacaag cgcctcgcgt tcagcgacga
     5701 aatcatgccg tttcgcgacg ccttcgacat agcgtttgtt gccggccttg agacgcgcca
     5761 gggcttcatc gggtgaaagt atgttttgcg gtttgggcgg cggcgtgtcg tcggccacgg
     5821 cggcaggcat cgcgagcagc gtgctgaagg cagcgccgag cgcgagcaca tcgcgacggt
     5881 cgaacgcact tttatgttgg cgtgttggaa agccgcaagt ctcgcacatg gttcgatctc
     5941 cggccgctgc tccaagttta gcattggcga tccaagtgcc gtcaaggtcg gatccaatca
     6001 tcatcaacgc tcaattctct ggccgcgcat agcgtttacg atattcggcg ggtgtcacgc
     6061 caacatggcg cgagaaagcg cgacgcaatg tatccgcatc cgcgaagccg cactttaccg
     6121 cgacctgctt tggcgcgacg cgaccggatt caagcatttg ccgcactgca tcaacgcgta
     6181 tgctttcaac ccacgctgcc ggcgtggcgc cgacctcgct gcgaaacaag cgcgcgaaat
     6241 ggcgcggact gaggtccgcc cgcttcgcga gagcagcgac attatgggtc ttggcgggat
     6301 tggcgataac ccaacgctga agttcctgta aggccgaacg gcccgcgggg gcggcttcac
     6361 ctttgcgact gaattgacgc tggccgccag gccgcttgaa aaacatgaca agctggctgg
     6421 cgactttcat agtaatgtcg cggcccatat cttcctcgac cagagccaag gccagatcca
     6481 ggccagctgt cacgcctgcg gcggtgcgca aacgtccgtc acgcagatag atcgcatcct
     6541 catccacgat gaccgacgga taacgctccg ccaatcgccc agctacagcc caatgtgtcg
     6601 tgacgcggca cccatcgagc agaccggcag cggcaagcag aaaagcaccg ctgcacactg
     6661 aaccatagcg acgcgcgctg gccgtcacat cacgcagcca ggcgaccatc gccggatcgg
     6721 ccgccgtgtc ggcgatatcc ggacaacccg cgacaagcaa agtgtgaatc ttttcgtcgt
     6781 gcagcgcacc catgacagca tccggcatca atcgaacgcc ggaggaactg cggattggag
     6841 ctgcctcagt gccgacgacg agcggtcgat agacaatctt gccggtctgg acattcgcct
     6901 ccgcgaaaac gtcgagcggg ccggaaacgt ccagtaattg gacgcccggc ggggcgagaa
     6961 ttgcgatggt tctgacggcg gccatgggtg tcagtttggg tccggttttg gcataaatcg
     7021 ccgtgatacg ttaattgctg acaacgtagg cgccttttac tttgctggca agacaagttg
     7081 aaaaggagag atgctaatga cgctcgatat ctcaggcgtg cgcatacctg acagcaaact
     7141 ggcgcgcgaa gtcaccgaac tggtgcgcgg caccgcctgg ccgcttttgt ttcatcactc
     7201 aagccgagtc tattatttcg gtgcgctcgc cggcaaacac cgcggtctta gtttcgatgc
     7261 ggagctgctt tatacgggcg ccatgttcca cgatacgggg ctgacggcgc agtacagcag
     7321 cgaccacgac cgtttcgaag tcgatggcgc caatgccgcg cgggattttc taaagcgcca
     7381 tgggatcgcg caaagcgaca tcgatacagt ctggacggcc atagcgctcc atacaacgcc
     7441 gggcattccg caatacatgc accccgtcgt ggcgctcgta acggcgggtg tcgaaatgga
     7501 tgtgcttggc ctgacctatc ccgaatatag cgacgtcgaa cgcgaagccg tggtccacgc
     7561 gcatccccgt acgccgcatt tcaaggaaga catcatccag gccttctacg acggcatcaa
     7621 gcacaagcct gagacgacat tcggtaatgt gaaagccgat gtgctggcgg ataaggatcc
     7681 gcattttcat cgcggcaatt tctgcagcgt gatccgcaac tcaccttgga aagggtgaac
     7741 cgcgggcggt tcgttaattt gcgaatctga aatgcagcac gtcgccgtcg gcgacgacat
     7801 aatccttgcc ttcgagacgg aacttgcccg cctcacgcgc gccagcctcg ccatttgcgg
     7861 cgacgtaatc ggcgtaggcg atcgtttcag cgcggataaa acccttctcg aaatcggtgt
     7921 ggatcacggc cgccgcctgc ggcgctttgg tgcccacttc gatcgtccac gcgcgggttt
     7981 ctttgggccc gaccgtgaaa tacgtgatga gatgcagcag cgcatagccg gcgcggatga
     8041 cgcgattgag ccccggctct tccagcccga ctgcttcgag ataatccttc tgctccgccg
     8101 ccggcagcag tgcgatctcg ctttcgatcg cggctgaaac gatgacggcg cccgcgcctt
     8161 cttgcgccgc gcggactttc acctgctcgg aataggcatt gcccttgtcg gccgacgctt
     8221 cctcgacgtt gcagacataa agaaccggct tggcgctcag caggcaaagc atgatgaagg
     8281 ccttctgctc ctcgggcttg atcgcggcga gacgtgccgg tttgccctcg cgcaacaaga
     8341 cgaggcaacg gctgacgata tcgaacgtct ccttggcctc tttatcgccg cccttggctt
     8401 tcttctccaa cgccggagcg cgcttttcga ggctgtcgag atccgccagc atcagctcgg
     8461 tttcgatcgt ctcgatatcg tcgagcggcg aaatcttgcc agcaacatgc gtgatgtcat
     8521 catcctcgaa acagcgcacg acatgggcga tggcgtcgca ttcgcggata ttggcgagaa
     8581 actgattgcc gagaccttcc cccttcgagg ccccgcgcac gaggccggcg atatcgacga
     8641 aggtaagccg cgtcgggatg atttctttgg agccggcgac cttcgccaga acttcgagcc
     8701 gcggatcggg cacggcgaca tcgccgacgt tcggctcgat cgtgcagaac ggataattgg
     8761 ccgcctgcgc cgccgccgtc tgcgtcagcg cgttgaaaag agtcgacttg ccgacgttcg
     8821 gcagaccgac aatgccgcat ttaaagccca tgaaagtctc ttctcggaag gaatggcgcg
     8881 agctttagca agcttcgccc gttattgcga gacgcctcac tccgccgcgg cgctgcggct
     8941 gccattgagc gccagcgcgc cggtctcgaa ctgcatcttg gcgagtttgg cgtaaagccc
     9001 gtccttggcg acgagcgatt gatgcgtgcc ttcctcgacg atgcggccgg cttcgaggac
     9061 gagaatacgg tcggcggaca gcacagtcgc cagccgatgc gcgatgacga gcgtggtgcg
     9121 gccctgtttc aactcttcga gcgcgtgctg gacgaggccc tcgctcgcgg catcgagtgc
     9181 cgaggtcgcc tcgtcgagca gcagcaccgg cgcctcgcgc aagatcgccc gggcgatggc
     9241 gagccgttgg cgctcgccgc ccgacagtgt gacgccgcgc tcgccgatat tcgtcgcata
     9301 gccttgcgac gtggcgacga tgaaatcctg cgcagcggct tttttcgcgg cggcttcgac
     9361 cgcggcttcg ctcgccgcgg gcgtcccgta gcgaatgttg tcggcgaagc tcgatccgaa
     9421 gatcaccggc tcctgcggca ccaggctgat gcggcggcgc agctcggccg gatcgaccgt
     9481 cttgatgtcg acgccatcga gatcaatctc gccgtcggtc ggatcataga aacgcagcag
     9541 aagctggaaa atcgtcgatt tgccagcgcc gctcggcccg acgatggcga ccgtctcgcc
     9601 tggcgcgacc cggaaagaaa gatcgtgcag cgccggggcg tcctgccggg tcggataagc
     9661 gaaattgaca tgatcgaaga cgatctcacc cttcccaggc aagggcaatt gcgcggcggg
     9721 caacggcgct gtgattttcg gcttgatcgc gaggatttcg ccgatgcggc cagcggcacc
     9781 ggcagccgcc gaaatctcgt tccagacctg gctcaattcg cccagcgcgc cggcaccgag
     9841 cacagcgtaa agcacgaatt gcgacagcag gccgcccgtc atacgtccgg cgagcacgtc
     9901 atgcgcgccc agccagagca caacgacgac actcgcgaag gcgaggaaaa tggcaatcat
     9961 cgtgacaatg ccgcgcgcca gcgtcgaaag ccgagcggca tcatatgagt tctcgaccgc
    10021 ggcggtgaag cgcgcgttgg tcgtcgcttc cgcgccaaac gcttgcatca cacgcacagc
    10081 accgagattt tccgccgcat aggcgctcgc atcggcgagc gtatcttgcg cggcgcgtga
    10141 gcgttttctg acggcgcgcc cggcggcata gagtggcagg acgatgatcg ggatcgcaac
    10201 gagcacatag gccgagagtc gcggactgct gtagaccatc agcgcaaccg cgccgatgaa
    10261 catgaaaaaa ttgcgtagcg cgacagaggc cgaggcgcca aaggccgcct tcatttgcgt
    10321 cgtatcggcg gtgagacgcg acagcagttc gccgattcgc gcgctgtcga agaaactcgc
    10381 gtcgagaccg gagagatgtc tgaagacagc gctgcgcaga tcggcgacga cgcgttcacc
    10441 cagcgtattg acgaggtaat agcgggcacc cgaggccagg gagagcacgc cgacgacgcc
    10501 gatcatcgcg ccgaaatagc tgtt
//
LOCUS       DASWOG010000124         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_57861,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000124 DASWOG010000000
VERSION     DASWOG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57861"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VGB93_02535"
     CDS             complement(<1..469)
                     /locus_tag="VGB93_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0145038.1"
                     /translation="MAEADFFLGRQVRLRQLPQGHRAGTDAVLLAAAAPVEVQGLALD
                     VGAGVGAAGLALARLRSNLTYGLIENDPATAALARENLALNDMAARGRVYEADVLDPD
                     SRRAVGLVDGSATLVITNPPFLDPARSRLSPDAGKRAAHAMPTPGPAALAEWLA"
     gene            complement(462..695)
                     /locus_tag="VGB93_02540"
     CDS             complement(462..695)
                     /locus_tag="VGB93_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY0145039.1"
                     /translation="MIELLRTNDLVLISAVEALLSENGIGVFVADQFASAIEGSLGFL
                     PRRVLILADDAAAARRVMREAGLSQELSSDRNG"
     gene            863..1768
                     /locus_tag="VGB93_02545"
     CDS             863..1768
                     /locus_tag="VGB93_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0145040.1"
                     /translation="MGVVVPLEEAAGESRIEALNRLAAPDMERVNQVILSHAASHVSM
                     IPDIANHLITSGGKRLRPMLTLATAGLCDYTGSGHIKLAATVEFMHTATLLHDDVVDQ
                     SNMRRGKLAARMLWGNEATVLVGDFLLGQAFKMMVEVGSLQALEVLSSAAVVIAEGEV
                     MQLAAAKDTQTSEDDYLAVIRAKTAALFGAASEVGPILAGRSKAEIAACRGYGLNLGI
                     AFQLIDDALDYGGKSAKLGKNVGDDFREGKITLPVVISFRRGTEAERACHYGAMAHDA
                     LELFPASPWKAALKDVVDFCIERAH"
     gene            complement(1832..2707)
                     /locus_tag="VGB93_02550"
     CDS             complement(1832..2707)
                     /locus_tag="VGB93_02550"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0145041.1"
                     /translation="MSQHLTDRAPAKINLTLHVVGRRADGYHELESLVAFSRTGDRLT
                     LEPGDKLELHVAGPTAGASGDPEKNLVIKAARQLAERVEGLHVGAFTLVKTLPVAAGI
                     GGGSSDAAAALRLLARLNALASDDPRLVEAARATGADVPVCVAAKARMMRGIGEKLGP
                     VLDLPPLPALIVNPRQPLETKPVFEFMKIAPGSDTHFGPQPTIASGMSYDALIAALRK
                     GRNDMEDAASVLAPIVAKVLAILTAAPGCRVARMSGSGATCFALFDTCRAVGRARKAI
                     AEAHPDWWVKATLLN"
     gene            complement(2819..4534)
                     /locus_tag="VGB93_02555"
     CDS             complement(2819..4534)
                     /locus_tag="VGB93_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145042.1"
                     /translation="MAKVNENLSIATPFEVGDSPTGNYLAAVVAGADRDTVAAATFFR
                     EALRFDPRNKELVERAFVAALSNGSMPEAFSLADRLLALEPDNGLAHLALGVRAIKEH
                     QYAAARSQFGRGGGNQGGDLTATLLSAWAYQGSGQTRRALELIGRLDDQNFGVFRNYH
                     QALIAAVAGRNDQAAQAFQQAYAADKNTLRLVDAYARFLSETGKNKDATDVYTAFSQV
                     VPDHPAVRAALADLKAGKTLAPLVKDVDQGAAEVLYGLGAAGGRQGDDLAGLIYLRLS
                     LYLDSDNSLALLTLGDLYERINQNEQALDVYQDVADSDPLRETADIEISQILENLGRS
                     DQALAHLRDVVNDHPYDEQALSALGNLQRAHDHYDEAIASYTRAIAASKKPETDNWPL
                     YYFRGIAYERSKNWPKAEADFKHALQLFPDQPLVLNYLGYSWVDKGVNLDQAFPMLRR
                     AVELRPTDGYIVDSLGWAEYKLGHYQEAVSDLEKAIDLKPADPVINDHLGDAYWHIGR
                     KLEAHFQWNHARDMGADASDLPRILQKIKFGLDKAPMKAGESKPVIGDKPTAADAGHK
                     LNGGG"
     gene            complement(4518..>4831)
                     /locus_tag="VGB93_02560"
     CDS             complement(4518..>4831)
                     /locus_tag="VGB93_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145043.1"
                     /translation="RRKMAIFGAMIRIESGRSCVNSAACFSGLPFRPIAGTLVSSAAG
                     LSQARKGCVRNADFRAQNTFIFRRIKLAFAPSRFACDFLFCFGCLDRVSGPFAGYGQG
                     E"
BASE COUNT          928 a         1505 c         1496 g          902 t
ORIGIN      
        1 cggcgagcca ttcggcgagc gccgccggtc ccggtgtcgg catcgcatgc gcggcacgtt
       61 tgcctgcatc gggcgaaagc cgtgaccgag cggggtcaag aaacggtgga ttggtaatga
      121 cgagcgtggc cgagccatcc acaagaccta cagcgcggcg gctgtcggga tcgagaacat
      181 cggcctcgta gaccctgccc cgcgccgcca tgtcattcaa agctagattt tcgcgcgcca
      241 gagccgccgt cgcgggatcg ttctcgatca atccgtatgt aaggtttgac cgcaaccgcg
      301 caagcgccag cccggcggcg ccgaccccgg cgccaacgtc cagcgccaag ccctggacct
      361 ccactggcgc tgcggcagcg agcaggaccg catccgtacc ggcgcgatgt ccctgtggca
      421 attgccgcag ccgcacttgc cgacccagga agaaatcggc ctcagccatt tcggtcactg
      481 gaaagttcct gcgataatcc ggcttcgcgc atcacccggc gcgcagccgc agcgtcgtcc
      541 gccaatatca ggacgcgccg cggcaaaaag cccagcgatc cttctatggc gctcgcgaac
      601 tggtcggcta cgaagacgcc gatcccgttt tccgataaaa gcgcctcgac agccgagatc
      661 agcacgaggt cgttggttcg tagaagttcg atcatggtcc ccgggcgcgg caattggaca
      721 tcggccgcac gctagacgtt ccgcttgctt tgcgccagtt gtcaccggtc gcagccgtgc
      781 tctggccctg gcttgcgggc cgcggtccgg cagcctaact tggcgccgcc caagtgcggg
      841 cggtcttccg ttggagagaa atttgggcgt agtcgttccg cttgaggaag ccgccggcga
      901 aagccgtatt gaagcgttga atcgcctcgc cgccccggat atggagcggg tcaatcaggt
      961 gatcctgtcg cacgccgcat cgcatgtctc gatgatcccc gatatcgcca atcatttgat
     1021 cacctccggt ggcaagcgtc tgcgcccgat gctgacgctc gcgaccgccg gcctctgcga
     1081 ctacaccggc agcggccaca tcaagctcgc cgccacggtc gaattcatgc acaccgcgac
     1141 attgcttcac gacgatgtcg tcgatcaaag caacatgcgg cgcggcaagc ttgcggcacg
     1201 tatgctctgg ggcaatgagg cgacagtcct tgtcggcgac tttttgctcg gccaggcctt
     1261 caagatgatg gtcgaggtcg gttcgcttca agccctcgaa gttctctcct cggctgccgt
     1321 cgtgattgcc gaaggcgaag tcatgcaatt ggccgcggcg aaggatacgc agacgagcga
     1381 agacgattat ctcgccgtca tccgcgccaa aaccgccgcg ctctttgggg ccgcctccga
     1441 agtcgggccg atcctcgccg gccgctcgaa agcggagatt gcagcctgcc gcggctatgg
     1501 gctcaatctc ggcatcgcct tccaacttat cgacgatgcg ctcgattacg gcggcaaatc
     1561 ggccaagctc ggcaagaatg tcggcgacga tttccgcgaa ggcaagatca cccttcccgt
     1621 ggtgatctcg ttccggcgtg gcacagaggc agagcgcgcc tgccactatg gcgccatggc
     1681 gcatgacgcg ctggaacttt tcccggcctc cccctggaaa gccgcgctta aagacgtggt
     1741 cgatttctgc atcgagcgcg cgcactaaac gcaggatccc gaaaagttgc agacttttcg
     1801 gacaagatca tgcgtcgtag agacccctcg ctcaattcag cagcgtcgcc ttcacccacc
     1861 aatcgggatg cgcttcggca attgctttgc gcgcccgccc gacggcgcgg caagtgtcga
     1921 acagcgcgaa acatgtcgcg cctgagccgg acatgcgcgc aacccgacag ccgggcgccg
     1981 ctgtcaggat cgcaagaacc ttggcgacga tcggcgcgag aacggaggcc gcgtcttcca
     2041 tatcgttgcg gcctttacgc agcgcggcga tgagcgcgtc ataagacatc cccgacgcga
     2101 ttgtcggctg cggtccaaaa tgcgtatcgc tgccgggtgc gattttcatg aattcgaaaa
     2161 cgggtttcgt ttcgagcggc tgacgcgggt tgacgatcag cgccggcaaa ggcggcagat
     2221 cgagcaccgg cccaagcttt tcgccgatgc cgcgcatcat ccgcgccttg gcggcgacgc
     2281 agactggaac gtcggcgccg gtcgcccgcg cggcctcgac gaggcgcggg tcatcggatg
     2341 caagagcgtt gaggcgcgcc aggagccgca gcgccgccgc ggcgtcggac gagccgccgc
     2401 cgatgcccgc cgcgaccggc agcgtcttca ccaatgtaaa agcgccgacg tgaagtccct
     2461 cgacacgctc ggccaattgc cgcgcagctt tgatgacaag atttttttcg ggatcgccgg
     2521 aagcgcccgc cgtcgggccg gcgacatgaa gctccagttt gtcgcctggc tcaagcgtca
     2581 gccggtcgcc ggtgcgcgag aaggccacga ggctttcgag ctcgtgatat ccgtcggcac
     2641 ggcgccccac gacatgcaac gtcagattga ttttcgcggg ggcgcgatct gtcaggtgct
     2701 gagacaaggc gggatcggct caaggatgga taattatgct ggaatcgtgg caagctggcg
     2761 aagaaatctc gtgtcagaga tgaagattgc ctcgccgcga ttgcgaaagt gagcgcgtct
     2821 agccgccgcc gttcaactta tgcccggcat ccgcggccgt gggcttgtcg ccgatcaccg
     2881 gcttggattc gcccgccttc atcggcgctt tatcgagacc gaatttgatc ttctgcagga
     2941 tacgcggcag gtcggatgca tcggcgccca tgtcgcgcgc atggttccac tggaaatgcg
     3001 cttcaagctt gcggccaatg tgccaatagg cgtcgccgag atgatcgttg atgacgggat
     3061 ccgccggctt cagatcgatc gccttttcaa gatccgagac ggcttcctga taatggccga
     3121 gtttgtattc ggcccagccg agactatcga cgatatagcc gtctgtcggg cgcagttcga
     3181 ccgcgcggcg caacatcgga aatgcctggt ccagattgac gcccttatcg acccagctgt
     3241 agccgagata gttcaggacg agaggctgat ccgggaaaag ctgcagcgcg tgtttgaagt
     3301 cggcctcggc ctttggccag ttcttgctgc gctcataggc gatgccgcgg aaataataca
     3361 acggccaatt gtcggtctcg ggcttcttcg atgccgcgat agcacgtgta tagcttgcga
     3421 ttgcctcatc gtaatgatca tgcgcgcgct gcagattgcc gagcgcagac agggcctgtt
     3481 catcgtacgg atgatcgttg acgacatcgc gcaaatgggc gagcgcctga tcgctgcggc
     3541 cgagattttc gaggatctgg ctgatttcga tatcggccgt ctcgcgtagc ggatcgctgt
     3601 ccgcgacgtc ctgatagacg tcgagcgcct gctcgttctg gttgatgcgc tcgtaaagat
     3661 ctccaagcgt cagcaacgcc agcgaattgt cagaatcgag atagagcgac aaacgcaaat
     3721 agatgagccc ggcgagatcg tcgccctgac ggccgccggc tgcgccgagg ccatagagca
     3781 cttccgccgc gccctgatcg acgtctttca ccagcggcgc gagcgtcttg ccggccttga
     3841 ggtcagccag cgcagcgcgc accgcgggat gatccggcac gacttgcgag aaagccgtgt
     3901 aaacgtcggt cgcgtctttg ttcttgccgg tctcgctgag gaatcgcgca taggcgtcga
     3961 caagccgcag cgtgtttttg tcggcggcgt aagcttgttg gaaagcttgc gcggcttgat
     4021 cattgcgacc ggccaccgcg gcgatcaaag cctggtgata attccggaac acaccgaaat
     4081 tctggtcgtc gagccggccg atgagttcga gtgcgcgccg cgtctggccc gatccctgat
     4141 aggcccaggc gctgagcaat gtcgccgtga ggtcgccgcc ctgattgccg ccgccgcggc
     4201 cgaactggct gcgggctgcc gcatattgat gctctttgat ggcgcggacg ccgagcgcga
     4261 ggtgcgctaa gccgttatca ggctcaagcg ccaggagcct gtcggcgagg ctgaaggctt
     4321 ccggcatgct gccattcgac aaagccgcaa cgaaagcacg ctctaccaat tctttgttgc
     4381 gtggatcgaa ccgcagggct tctcgaaaga acgtcgccgc tgccactgtg tcgcgatccg
     4441 cgcctgcaac gaccgccgcg aggtaattgc cggtcgggct atcgccgact tcaaacgggg
     4501 tcgcgatgga taaattctca ttcaccttgg ccatagccgg cgaaaggccc gctgacgcga
     4561 tcaaggcagc cgaagcaaaa gagaaaatcg cacgcgaagc gagacggtgc aaaagcaagc
     4621 ttaatcctcc ggaaaataaa cgtattttgg gcgcggaagt cagcgtttcg cacgcagccc
     4681 ttgcgagcct gggacaggcc tgctgccgag ctaaccaagg tgccagctat aggccgaaac
     4741 ggcaagcccg aaaagcaggc cgcgctgttg acgcaggaac gccccgattc aattcggatc
     4801 atggccccga aaatggccat tttacgtctg c
//
LOCUS       DASWOG010000125         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_241139,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000125 DASWOG010000000
VERSION     DASWOG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241139"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGB93_02565"
     CDS             complement(<1..332)
                     /locus_tag="VGB93_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145044.1"
                     /translation="MISETALDLAVARDVLSAAQAEELRSLARTEAEAVRANLPPPPP
                     IPPPIPVSAAVAPDANAFDDESLRFITSFADIFVTLGIALFCWAAADITSHYVGDTAK
                     WGVVAVLA"
     gene            complement(329..895)
                     /gene="hslV"
                     /locus_tag="VGB93_02570"
     CDS             complement(329..895)
                     /gene="hslV"
                     /locus_tag="VGB93_02570"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0145045.1"
                     /translation="MSDQSPRNESWHGTTIIMVRKGGQTVIGGDGQVTIGQTIIKGNA
                     KKVRRLAKGEVIGGFAGATADAFTLFERLEAKLEQYPGQLVRACVELAKDWRTDRYLR
                     RLEAMMLVADKTAGLVLTGNGDVLEPEATLDGSVMGIGSGGNYALAAGRALLSTETDA
                     ETIARRALDIAADICVYTNRNVVIESLL"
     gene            1122..1715
                     /gene="hisB"
                     /locus_tag="VGB93_02575"
     CDS             1122..1715
                     /gene="hisB"
                     /locus_tag="VGB93_02575"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY0145046.1"
                     /translation="MRKAAIARKTKETALEVRVDLDGTGHANIATGVGFFDHMLEQVA
                     RHSLIDIDVAAQGDLHIDQHHTVEDTGIALGQAIRQALGDARGIIRFGEALQPMDETL
                     TRVAIDLSGRPYLVFRTTFSRQKIGEFDTELVREFFQALTTNAGMNLHIETLYGENNH
                     HIAESAFKGLARALRSAVAIDPRRSHEIPSTKGSLAG"
     gene            1838..2347
                     /locus_tag="VGB93_02580"
     CDS             1838..2347
                     /locus_tag="VGB93_02580"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEY0145047.1"
                     /translation="MAVYTVYVPNFGRRTLPSDAEKYAALPDAVFVREGFSRAAFFLG
                     PFWLAWNRLWIALVAWFAIYFLLTIEAPEVLGGGSIFWTGLLLEFLLGLEGNSLRRWE
                     LARRGFRLTDIAAGLRRTDAERSFFSRALRAIPPPAPTPKISDAVPAFAPPPSSEVVG
                     LFPRPEDAR"
     gene            2344..2994
                     /gene="hisH"
                     /locus_tag="VGB93_02585"
     CDS             2344..2994
                     /gene="hisH"
                     /locus_tag="VGB93_02585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0145048.1"
                     /translation="MNVAIVDYGSGNLHSAHKAFERAAREAGLNVEIKVTGEPDDIRH
                     ADHIVLPGVGAFADCRRGLDAVPGMIEALDERVRKDGQPFLGICVGMQLMATRGLEYE
                     TTDGLGWICGDVAAIEPSDASLKIPHMGWNTLVTEKAHPLLANIPTGPDGLHAYFVHS
                     FAYTNVPKDEIVASTDYGGPVVAIVGRDNIVGTQFHPEKSQKLGLALIANFLRWQP"
     gene            2991..>3262
                     /locus_tag="VGB93_02590"
     CDS             2991..>3262
                     /locus_tag="VGB93_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0145049.1"
                     /translation="MILFPAIDLKNGECVRLLYGDMDKATVFNKDPAAQAEAFERQGF
                     TYLHVVDLDGAFAGKPMNASAVDSILARVKMPVQLGGGIRDLRTVEG"
BASE COUNT          623 a         1006 c          996 g          637 t
ORIGIN      
        1 cacgccagca cggcgacgac gccccatttc gccgtgtcgc ccacataatg cgaggtgatg
       61 tcggccgccg cccagcagaa aagtgcaatg ccaagcgtca cgaatatatc ggcgaagctt
      121 gtgatgaaac gcaggctctc gtcgtcgaaa gcattcgcgt cgggggctac ggccgctgat
      181 accggaattg gcgggggtat cggcggaggc ggcggaagat tggcgcggac ggcttcagcc
      241 tcggtgcgag cgagcgagcg caattcctcc gcctgggcgg cactcagaac gtcgcgtgca
      301 acggcaagat cgagcgccgt ctcggagatc acagcaagct ctcgatgacg acattccggt
      361 tggtatagac acaaatgtcg gcggcaatat cgagcgcgcg gcgggcgatc gtctcggcat
      421 cggtctccgt cgacagaagc gcgcggccgg cggcgagcgc ataattgccg cctgagccga
      481 tacccatgac gctgccgtcc agcgtcgcct cgggctccag cacgtcgcca ttgccggtca
      541 gaaccagacc tgccgtcttg tcggcgacca gcatcatggc ttcgagccgg cgcagataac
      601 ggtctgtgcg ccaatccttg gcgagttcga cgcaggcgcg caccaattgg ccgggatatt
      661 gctcgagctt ggcctcaagc cgttcgaaca gggtgaacgc gtcagctgtt gcgccggcaa
      721 agccgccgat cacctcgccc ttggcaaggc ggcggacctt cttggcattg cccttgatga
      781 tcgtctggcc aatcgtgacc tgcccgtcgc cgccaatgac ggtctggccg cccttgcgca
      841 ccatgatgat ggtcgtgccg tgccaggatt cattgcgggg agattggtcg gacatcgtct
      901 ctttcaaaaa gccgcggcgg cgcgtggcaa aactcgctcc catctatgaa tttgccgcgc
      961 cgctggcaag ggcgcgaccg gcacaccggg tacgcattca agccaaaaaa taaagcatat
     1021 cagaggcttc cgcctcgtcg gcgcggcttt ggttttctct ttgcctaaat atgttctatt
     1081 gtccggcacc gcattccctg gacagaagac cctaatttcc catgcgcaaa gctgcgattg
     1141 cccggaagac caaagaaacc gcccttgagg tccgcgtcga tctcgacggg acgggccatg
     1201 ccaatatcgc caccggcgtg gggtttttcg accacatgct cgaacaggtg gcgcgccatt
     1261 ccctgatcga cattgatgtt gcggcgcagg gcgacctgca tatcgaccaa catcacactg
     1321 tcgaggatac ggggatcgcg ctaggccagg cgattcgtca ggcgctcggc gacgcgcgcg
     1381 gcatcatccg cttcggtgag gcgctccagc cgatggatga gacattgacg cgggttgcga
     1441 tcgatctctc ggggcgtccg tatctcgtct tcaggaccac gttctcgcgc cagaagatcg
     1501 gcgagttcga caccgagctc gtgcgcgaat tctttcaggc gctgacgacc aacgccggga
     1561 tgaatctcca catcgaaacc ctatatggcg agaacaacca tcatatcgcc gagtccgcct
     1621 tcaaagggtt ggcgcgggcg ttgcggagcg ctgttgccat cgacccgcgc cgcagccacg
     1681 aaatcccctc gaccaaaggg tcgctggcgg gctagagcat cggactgaaa agtgcgaagc
     1741 ggttttctga tcaatccggt gcgattgata aaggcatacc cgcactgttc gcttcgccgt
     1801 aatgacggcg agacaacgga taggaatcac agaggaaatg gccgtctaca cggtttacgt
     1861 tcccaatttc ggccgacgga cactcccgtc cgacgccgag aaatacgcgg ctttgcccga
     1921 cgcggtcttc gtccgcgagg ggttttcgcg cgcggcgttt ttcctcgggc cgttctggtt
     1981 ggcctggaat agactttgga tcgctctggt cgcgtggttc gcaatctatt ttctactcac
     2041 gatcgaggcg ccggaggtcc tcggcggtgg atcaatcttc tggaccgggc tgctgctcga
     2101 atttctgctc gggcttgagg gcaacagcct tcggcgctgg gaacttgcaa ggcgcggctt
     2161 ccgcctcacc gacattgcgg caggccttcg gcgcaccgat gccgagcgca gctttttcag
     2221 ccgcgccttg cgcgcaattc cgccgcccgc accgacgccc aaaatcagcg atgccgtgcc
     2281 agcctttgcg ccaccgccgt cgagcgaggt ggtgggcttg tttccacgtc cggaggatgc
     2341 gcgatgaatg tcgcgatcgt cgattacggc tccggcaatc tgcactcggc gcacaaagcg
     2401 tttgaacgcg ccgcgcgcga agcaggtctg aatgtcgaaa tcaaagtcac tggcgagccg
     2461 gacgatatac gccacgccga tcatatcgtg ctgcccggcg tcggcgcctt tgccgattgt
     2521 cgtcgtggcc ttgatgcggt gcccggcatg atcgaagcgc tcgacgaacg cgtgcgcaaa
     2581 gatggacagc cgtttctcgg catttgcgtc ggcatgcaat tgatggccac gcggggtctc
     2641 gaatacgaaa caaccgatgg cctcggctgg atctgcggcg acgtcgccgc gatcgagccg
     2701 tccgacgcga gccttaaaat tccacacatg ggctggaaca cgctcgtgac ggagaaagcg
     2761 catccgctgc ttgcgaatat tcccaccggg ccggacgggc tgcacgctta tttcgtccac
     2821 tcgtttgcct atacgaatgt gccgaaggac gagattgtcg cctcgaccga ttacggcggc
     2881 ccggttgtcg cgatcgtcgg gcgcgacaat atcgtcggta cgcaattcca tcccgagaaa
     2941 agccagaagc tgggcctcgc cttgatcgcc aatttcctcc ggtggcagcc gtgatcctgt
     3001 ttcccgcaat cgacctcaag aacggcgaat gcgtccgctt gctttatggc gacatggaca
     3061 aggcgacggt gttcaacaaa gaccccgcag cgcaggccga ggccttcgag cggcagggtt
     3121 tcacctatct gcatgtcgtc gatctcgacg gtgctttcgc cggcaagccg atgaatgcaa
     3181 gtgctgtaga ttccatcctc gcccgggtga aaatgccggt gcagctcggc ggcggcattc
     3241 gcgatctacg cacagtcgag gg
//
LOCUS       DASWOG010000126         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_351187,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000126 DASWOG010000000
VERSION     DASWOG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351187"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VGB93_02595"
     CDS             <1..490
                     /locus_tag="VGB93_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0145050.1"
                     /translation="RDKIAARYPLNKYPSPALAEVAVAQGFKSCTARLLDRAWAKYAP
                     VYAYEFRDQTAPSYFPKFSFPMRAYHTAELQYIFPLFHGGQGTPHALNSAQEKLSDQM
                     VDYWTSFARTGRPTSHSSQPWKPYDAQTDNYQVLDLSVVSNAGGYGKKYDCELWDAIL
                     SL"
     gene            603..>1950
                     /locus_tag="VGB93_02600"
     CDS             603..>1950
                     /locus_tag="VGB93_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0145051.1"
                     /translation="MLSLIDELLKEQFPQTIDGTDAASVFEDVINPATGRPFHGAPVA
                     TVDELEAALAAARRAQPSWAARSWEERAAALESFAKTIETNVERLAHLFTLEQGRPLA
                     GARIELGLACRYIRLLAAVRLPSRVLIDNEVRRIVERWTPKGVVLAVAPWNAPVVLGM
                     AKIATALIGGNTIVIKPSELTPLCTLELGRLGRKVFPPGVLNVVSGGRAVGAALVELP
                     GFDKISFTGSTATGRSIAAKAGTLLRPVTLELGGNDAAVLLPDGSIEAMTAAATLTGL
                     VNCGQFCGAVKRIYAPRNLYEAVCESLTERAKKIVIGDGLSPGVEMGPIQNKAQFDKV
                     CAIVDDARAAGGRILTGGEPMASDGYFYPPTVVADLTNGVRLVDEEQFGPVIPIVAYD
                     DLDHVIDILNAGPYGLTSSIWTADLDHGAELASRLAVGTGLVNQHGGFDASVPMSLI"
BASE COUNT          393 a          600 c          565 g          392 t
ORIGIN      
        1 tcgagataaa atcgcagccc gctatccgtt gaacaaatat ccgagcccgg ccttggcaga
       61 agtggcggtc gcgcaaggct tcaaatcatg taccgcgcgc cttcttgacc gagcttgggc
      121 gaaatacgcg ccggtctatg cctatgaatt ccgcgaccaa acggcgccgt cctattttcc
      181 caaattcagc tttccgatgc gcgcatatca tacggccgaa ctgcaatata tcttccccct
      241 ctttcacggc ggccagggta cgccgcatgc gctgaactcg gcgcaggaga aactttcgga
      301 tcagatggtg gattattgga caagcttcgc gcggacgggc agacccacga gccactcaag
      361 ccagccgtgg aagccttacg atgcgcaaac cgataattat caagtgctgg atctgtcggt
      421 cgtgagcaac gccggtggct atggaaagaa atacgattgc gaactttggg acgcgattct
      481 ttcattatga tccgatccat cagagttcgc gacataactg aattgcggga tcataaccta
      541 gacgcctatt ccggcggccc atacagacga aactcccgcc ttggcttccc tcggaacaac
      601 gaatgctaag cctcatcgat gagctactga aagaacaatt cccgcaaacg attgatggca
      661 ccgatgccgc gagcgtcttt gaggatgtca taaatccagc gaccggccgt ccctttcacg
      721 gcgcgcccgt agccacagtg gatgagttgg aagccgcgtt ggcggcggcc cggcgcgcgc
      781 agccgtcgtg ggccgcgcga agctgggaag aacgtgccgc ggcgctggaa agcttcgcca
      841 agaccatcga gacaaatgtc gagcgcttgg cgcacctttt cacgctcgaa caagggcgtc
      901 cgctggccgg ggcccgtatc gagcttggac tcgcttgccg atatattcgc ctcctagcgg
      961 cggtgcggct tccttcacgc gtccttatcg acaatgaggt ccgccgaatt gtcgagcgat
     1021 ggacgccaaa aggcgtcgtg ctcgccgttg cgccgtggaa cgcgccggtc gtgcttggca
     1081 tggcgaaaat cgccactgcc ctcatcgggg gaaacacgat cgtcatcaag ccgtcggaat
     1141 taacgccgct ctgtaccctc gagcttggtc gactcggccg gaaggttttc ccgccgggcg
     1201 ttctcaacgt cgtctcgggg ggccgcgcgg ttggtgcggc tttggtcgaa cttcccggct
     1261 tcgacaaaat ctccttcacc ggatcgacgg cgacgggccg ctcgatcgca gccaaggccg
     1321 gaacgctttt gcggccagtc actctggaac tcggcgggaa cgacgctgcg gtccttctgc
     1381 cggacggatc aatcgaggcc atgacggcgg ccgcgaccct gaccggcctt gtgaattgcg
     1441 gccagttctg cggcgccgtc aaacgcatct atgcgccccg caatctttat gaagcggtct
     1501 gcgaatcttt gaccgaacga gccaaaaaaa ttgtgatcgg cgacggcctg tcacccggcg
     1561 tcgagatggg tcccatccag aataaggctc agttcgacaa ggtttgcgcc atcgtcgatg
     1621 acgcccgtgc tgccggcggc cgcattctta ccggcggcga acccatggct agcgacggat
     1681 atttctaccc gcctacagtg gtggcggacc tcaccaacgg cgttcgacta gtcgacgagg
     1741 aacagtttgg gccggttatt cccatcgtgg cctatgacga tctggatcac gtgatcgata
     1801 ttctcaacgc cggcccctat ggcctcacca gttcgatctg gaccgctgac ctcgatcacg
     1861 gagcggagct ggcatcacgc ctggccgtcg gcacgggtct ggtcaatcag cacggcggat
     1921 tcgacgcgtc cgttcccatg tcgctcatca
//
LOCUS       DASWOG010000127         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_113116,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000127 DASWOG010000000
VERSION     DASWOG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113116"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VGB93_02605"
     CDS             complement(<1..918)
                     /locus_tag="VGB93_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0145052.1"
                     /translation="MTVRKSISVYHQPAGGWGALKAVAEALDEQGIAVKGTTSRVRMN
                     QPEGFDCPGCAWPDPKHTSSFEFCENGAKAIAWEATAKRCTPEFFAAHTVTELDTWSD
                     YDLEMVGRVTHPMVYDAASDHYKPISWDGAFTIIGRHLNGLSNPNAADFYTSGRASNE
                     AAFLFQLFAREYGTNNFPDCSNMCHEASGVALEQAVGIGKGSVLLDDFEKADAIFVIG
                     QNPGTNHPRMLGDLRRAALRGAKIAVFNPVREKGLERFADPQDKLEMITGGSTPIASE
                     YYQVRLGGDMAAVRGMSKAVFAADDAALAA"
     gene            1269..1442
                     /locus_tag="VGB93_02610"
     CDS             1269..1442
                     /locus_tag="VGB93_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145053.1"
                     /translation="MSAAIREPASDDSSFGAAGLRDGNWLELPTFYPDALGFAARTSI
                     AILCLLCFVLGAD"
     gene            1515..>1822
                     /locus_tag="VGB93_02615"
     CDS             1515..>1822
                     /locus_tag="VGB93_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0145054.1"
                     /translation="MRDFGAYGAVLCGYTAGAIAVIGFWHYYLVGSWTRDGEVEANVV
                     NLSPQIFGRIIRIPVRDNQTVRKGDILYEIDPAGLQIAVDVAAANVQSTLADATLKRA
                     "
BASE COUNT          358 a          513 c          532 g          419 t
ORIGIN      
        1 cgcggcgagc gccgcgtcat ctgctgcgaa cacggctttg gacatgccgc gtaccgccgc
       61 catgtcgccg ccgaggcgca cctgataata ctcgctggcg atcggtgtgc tgccgcccgt
      121 gatcatttcg agcttgtcct gcgggtcggc gaagcgttcg aggccttttt cccgcaccgg
      181 attgaagacg gcgatcttgg cgccgcgaag cgcggcccgg cgaaggtccc caagcatgcg
      241 cggatggttc gtgccggggt tctggccgat gacgaaaatg gcgtcggcct tttcgaagtc
      301 atcgagcagc acggagcctt tgccgatgcc gacggcctgc tcgagcgcga cgcccgaggc
      361 ttcgtggcac atgttcgagc agtcagggaa attgttcgtg ccgtattcgc gagcaaaaag
      421 ctgaaagaga aatgccgcct cattggaggc gcgcccagag gtataaaagt ccgcggcgtt
      481 tggattgctg aggccgttca ggtgacggcc aatgatggta aaggcgccgt cccaagagat
      541 cggtttatag tgatcggacg cggcgtcgta gaccatcggg tgagtgacgc gtcccaccat
      601 ttcgagatcg taatcgctcc acgtgtcgag ttccgtaacg gtgtgagcgg cgaagaattc
      661 cggcgtacac cgttttgctg tcgcctccca cgcgatcgcc ttcgcaccgt tttcgcaaaa
      721 ctcgaaggat gacgtatgct taggatccgg ccacgcgcag ccgggacaat cgaagccctc
      781 gggctgattc atccgcacac gcgatgtggt gcctttgacg gcgatgcctt gctcgtcgag
      841 cgcttcggcg actgctttta gcgcacccca tccgccagcc ggctggtgat atacggagat
      901 cgatttacgg acagtcatgc gcaaacctct gcacaagctt ggccggagcc aaacgacaag
      961 cttggatttc agactccctc gaacctatca tagctcctga gtttgatgac gaccgaaaac
     1021 gcccgaaaaa ttccgatttc gcacgatgca ataaagtaat tcacgtacca gaattttgtc
     1081 tatcgcgcat gtgcccgttc ggaaagattt tgggatcgat gtcgctcgaa gcgctcggcc
     1141 aagagtccgt gctatatacg agactactca gcatgcgcgc tgcgccacat gtaggattcc
     1201 cgtcggctga cgcttggcgg agtggaaact catttcaaaa gccgtaatct cagctcggag
     1261 gaaatatttt gtcagccgca attcgcgaac ccgccagcga cgacagcagt ttcggagctg
     1321 ccggtctccg cgacggaaac tggcttgaat tgccaacgtt ttatccggat gcgcttggct
     1381 ttgcggctcg gacatcgatc gccattctgt gcctactttg tttcgttttg ggcgcggatt
     1441 gaaatacccg cgtcagccgc tgctgatagc attctgctta ttggctcggt ctctgttcgt
     1501 tcatcgcatc tttgttgcga gacttcggtg cttatggcgc cgtcctttgc ggctacaccg
     1561 ccggtgcgat cgcggtgatc ggtttttggc attattatct cgtcggttcc tggactcgcg
     1621 acggcgaggt cgaggcaaat gttgtcaatc tctcaccgca gatcttcggc cgcattattc
     1681 gaattcctgt tcgagacaat caaacggttc gcaagggcga catactttat gagattgatc
     1741 ctgcgggttt gcaaattgcc gtggatgtcg ccgcggcgaa tgtccagagc acgcttgccg
     1801 atgcgacgct gaaaagagcc ga
//
LOCUS       DASWOG010000128         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_57949,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000128 DASWOG010000000
VERSION     DASWOG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57949"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VGB93_02620"
     CDS             <1..1152
                     /locus_tag="VGB93_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145055.1"
                     /translation="TFFLADVQTGFGPFVAVYLTTAQWTQQDIGFILSITGIIGLLGR
                     MPGGAIVDAARSERNVAAIAVVCIGISALMLAAWPLFPLVFLSTVLSSSANCVLAPAI
                     GAISLGLVGHFAIGPRLGRNARFASLGTVIAAAGMGIGGHFFSSRSVFVITALLLGPT
                     LIALWQIRPTEVDPTRAHGGNKPLEHPERNLLRLALNGPLLAFAVCNLLFQLANAAML
                     PLMGSILTMRSANWATVLIAACIIVPQLIVAALSPWVGRRADKWGRRPILLIGFGALP
                     LRCLLFAYMPDPSFVVFEQILDGISAAAIGVMVPLVVADISRDTGRFNLAQGVIGTAV
                     GIGAALSTSFAGYVSDHSGSQSAFLDLAAIAACGFAFVLFVMPETRPRR"
     gene            complement(1156..2214)
                     /locus_tag="VGB93_02625"
     CDS             complement(1156..2214)
                     /locus_tag="VGB93_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0145056.1"
                     /translation="MRIAQIAPLYEAVPPRLYGGTERVVSWLTEELVALGHDVTLFAS
                     GDSRTSATLAPMWPCALRLDGSVREPGALHIAMLEHVYQRSHEFDLLHFHLDYFPFSL
                     FTRQSTPFVTTLHGRLDLPELQPVFKTFSSLPLVSISDSQRRPAPNARWAATIHHGMP
                     ENLLTPIPAEPRYLAFLGRISPEKRVDRAIQIAIRAGLPLKIAAKVDAADVDYFEEQI
                     RPLLAHDNIEFIGEIGDVQKPEFLSGAMALIVPIDWPEPFGIVMIEAMACGTPVIAFN
                     RGSVPEVIEDGVTGFIVEDETSAVGAVRRLSQLSRPQIRKRFEERFTARRMAKDYIGV
                     YNELISAATPRPLAVARF"
     gene            2493..>2824
                     /locus_tag="VGB93_02630"
     CDS             2493..>2824
                     /locus_tag="VGB93_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145057.1"
                     /translation="MDDLCQYADRPTGFVLRYVKLQPISHLIILICVLAAVGCSVSTQ
                     FGIKFLVDSLSKGVDHANPWTAFAILCGFIAADNLLWRVAGWIAASSFVRVTGDMRRE
                     MFQHLMGHS"
BASE COUNT          534 a          824 c          834 g          632 t
ORIGIN      
        1 acgtttttcc tcgccgacgt ccagacgggc ttcgggcctt tcgttgctgt ctatctgacc
       61 acggcccaat ggacgcagca ggatatcggt ttcatcctgt cgatcaccgg aattataggg
      121 ctgctcggcc ggatgccggg cggtgctatt gtggacgccg cgcgctcgga gcgtaacgtc
      181 gccgccatcg cggttgtctg tatcggcatc agcgcgttga tgcttgcggc gtggccgctt
      241 tttccgttgg tctttctctc gacagtcctc agctcgagcg caaattgcgt gctcgcgccg
      301 gcgatcgggg cgatcagtct cggcctcgtc ggccattttg cgatcgggcc acgtctcgga
      361 cgcaatgcgc gctttgcctc gcttggcact gtcatcgcag cagcgggcat gggtatcggc
      421 ggacacttct tctcaagccg atccgtcttc gtcatcacgg cacttcttct cggcccgacc
      481 ctgatcgcac tttggcagat tcgccccacg gaagttgatc ccacccgggc acatggcggc
      541 aacaagccct tggagcatcc tgagaggaat ctcttacgtc tcgcattgaa cgggccgttg
      601 ctcgccttcg cggtttgtaa tttgctcttt caattggcca acgcagcgat gctgccgctc
      661 atgggcagca ttttgacgat gcgctccgcc aattgggcga cggttctgat tgccgcgtgc
      721 atcatcgttc ctcaactgat cgtcgcggcc ctgtcgcctt gggttggacg ccgcgccgac
      781 aaatgggggc gtcggccgat cctcttgatc ggcttcggcg cattaccgct gcgctgtctc
      841 ctgtttgctt atatgcccga cccgagtttc gtcgtcttcg agcagatttt ggatggaatt
      901 tcggccgcgg ctattggcgt gatggtgccg ctggtcgtcg ccgacatttc gcgcgacacc
      961 gggcgcttca atctcgcgca aggcgtcatc ggcactgcgg tcgggatcgg cgccgcgctc
     1021 agcacgtctt ttgccggata tgtgagcgat cattccggca gccagagcgc gtttttagat
     1081 ctggctgcca tcgccgcttg cggctttgct ttcgtcttgt ttgtgatgcc agagacgcga
     1141 ccgcgtcgat gaaggtcaaa acctcgcgac ggctaacggg cggggcgtcg ccgcactgat
     1201 caattcatta taaacgccga tataatcctt ggccatgcgg cgcgccgtaa accgctcttc
     1261 aaaacgcttg cgaatttgcg gccttgagag ctgtgataag cgacggaccg cgccgaccgc
     1321 actcgtttcg tcttcgacga tgaagccggt cacgccatcc tcgatcacct cgggaactga
     1381 gccgcggttg aacgcgatca cgggcgtacc gcacgccata gcctcgatca tgacgatccc
     1441 gaagggctcc ggccagtcga tcggtacgat gagagccatc gcgccgctaa ggaattcggg
     1501 cttctgcacg tcgccgattt caccgatgaa ctcgatattg tcatgcgcga gaagcgggcg
     1561 aatttgctct tcaaaataat ccacgtccgc tgcatccact ttcgcggcga ttttaagtgg
     1621 caggccggca cgtatcgcaa tctggatcgc gcgatcgacg cgcttctcgg gggaaatacg
     1681 accgaggaac gccagatagc gcggctcggc gggaatcggc gtcagcagat tttccggcat
     1741 cccgtggtgg atcgtcgctg cccatcgggc atttggcgcc gggcggcgct gggagtccga
     1801 aatcgataca agaggcaacg aggagaacgt cttaaaaaca ggctgcagct caggcaggtc
     1861 aaggcggcca tggagcgttg tcacgaacgg cgtcgattgc cgagtgaaga gcgaaaacgg
     1921 gaaataatcc aaatgaaaat ggagaagatc aaattcatgc gagcgttgat agacgtgttc
     1981 gagcattgca atatggagcg cgcccggttc gcgaaccgat ccatcgagac gcagggcgca
     2041 gggccacatg ggcgcaagcg ttgccgatgt gcgcgagtcg ccgctcgcaa atagagtgac
     2101 atcatgcccg agcgccacga gctcttcggt aagccacgaa acgacacgtt ccgtgccgcc
     2161 ataaagacgc ggcggaacgg cttcataaag aggcgcaatc tgggcaatac gcattggaat
     2221 caatccctcg tgaacgacat ttcgaagcgc aatcgcggac tgtcgcacgg ttacagaact
     2281 agcgatctgt aaatcgcctg caatcgcgcg cccgaaactg cagagacaac gcgcaacggc
     2341 ctagtttgtt caaacttttc ggcactgcac acattcgaca ggaacttaag gctgcgcttg
     2401 gcgtttgctg tcacccggtc gcataagcaa gtctctgtcc gatatcgccg gtattcaatt
     2461 tgtagcgaat cccgaccggg ggaaggtccc gaatggacga tctttgccaa tacgccgacc
     2521 ggccgacggg ttttgttctt cgctatgtca agcttcagcc gatctcgcat ttgatcatat
     2581 tgatctgcgt gctcgcggct gtgggctgct cggtcagcac gcaattcggc atcaagtttt
     2641 tggtcgatag cctgtcgaaa ggcgtggacc atgccaatcc ctggacagcc tttgcgatct
     2701 tgtgcggctt catcgcggcc gacaatctgt tgtggcgtgt cgcggggtgg attgcggcca
     2761 gttcattcgt tcgcgtcacg ggcgatatgc gccgcgaaat gttccagcat ttgatgggcc
     2821 attc
//
LOCUS       DASWOG010000129         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_21509,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000129 DASWOG010000000
VERSION     DASWOG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21509"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1058
                     /locus_tag="VGB93_02635"
     CDS             336..1058
                     /locus_tag="VGB93_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0145058.1"
                     /translation="MTEDKLRHALVDASRELEKLQINQGTSGNLSARSGDAMLITPSG
                     IPYDKLTPEMIALMPMSVNDGAWRGPQKPSSEWRMHLDILCARPDDGAVIHTHSVYAT
                     ALSMLREEIPAAHYMVAAFGGPNVRCTPYAPYGTQELSDLVIEGLRDRHGVLLGQHGM
                     VVTGRDLGQAMWRAVELETLAKMYVIARSIGRPHILPSEEIAITVERFKSYGYQSDAV
                     AALVAAPRTSPSKKSAGKPKKL"
     gene            complement(1060..1500)
                     /locus_tag="VGB93_02640"
     CDS             complement(1060..1500)
                     /locus_tag="VGB93_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0145059.1"
                     /translation="MEYLHTMVRVTDLDQSLDFYCDKFGLVEMSRIVNEKGRFTLVYL
                     AAPGDAEAAKTTKRPLIELTYNWDPETYTGGRNFGHLAFAVDDIYATCEKLQNAGVTI
                     NRPPRDGHMAFIRSPDNISIELLQKGGAKPAQEPWHSMSNTGVW"
     gene            1726..3096
                     /locus_tag="VGB93_02645"
     CDS             1726..3096
                     /locus_tag="VGB93_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0145060.1"
                     /translation="MIENNYIKERAALVSVGVKTLLAIAKLVAGLMSGSLALLSEAAN
                     NIGDIAIVCFSFVAIRIANKPADDTHHYGHAKVETLAALAQTGFLLGLAVYIFATAVN
                     RLMIGYVNVVPGALSFGVLVVSIIVDISRWYSLNKIAKKTRSEALAADALNFASDIVA
                     STFALLGLLAVHFGFWQGDSIGALGVAVFIAIAGFNLARRTINALTDAAPPGLTESIS
                     AAALRVPGVIAVDSLRLRPVGPNVLGDISIRVPRTLPQDGVTAIKGNVSAAIAATHPD
                     VELTVETTPVALDDESVVERLLLIAAKRHVAIHHVIVQQVCGKIALSCDIEVDGTMPL
                     GQAHSIASGLEAEARKELGPDIEIDTHIEPLEPRELAGEDAPGETRVAIANALSAAAS
                     DGIEDIHNVRARKTASGLVVNYHCRADPRASVAAVHQAVDALEHRVREEFPAIARLVG
                     HAEPLR"
     gene            3103..4389
                     /locus_tag="VGB93_02650"
     CDS             3103..4389
                     /locus_tag="VGB93_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145061.1"
                     /translation="MFLSLLRSRRFAGLFWCQFLSAFNDNFVRNLLVTLILFRLGREN
                     AGALITLAVGVFILPSVFLSGLAGELADAHDKALVARRLKIGEIFVQAIAAAGVWFGS
                     LPLLYVALCGLGTIAALFGPVKYGFLPEHLETRELPAGNALVEAATFLAILLGLVAGA
                     LAADKTLAPGTIVVQLMLVAAAAYATSLFIPRKSAAAPDLPVHRNILASTVELVRELQ
                     AEPKLWRPGLAVSWFWLTGAVALSLVPVAVRNATGGGIAVEAAISGFFAFGIGAGSIA
                     AALLARGRISLAPAPAAAIAMAAFLLALAWSTRGLPAPHTADTSLGSFFATARGLMIA
                     VEVFGLAAAGGLFVVPLFSAIQARAAPDRRARTVAAVNIQNALFMVAGSLLTASLQSR
                     FFGLGEPTLLALLGFGNVAAAAYIRRAIIGAEAKSG"
     gene            4468..>6792
                     /gene="pepN"
                     /locus_tag="VGB93_02655"
     CDS             4468..>6792
                     /gene="pepN"
                     /locus_tag="VGB93_02655"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0145062.1"
                     /translation="MRTDVAQPIRLADYRAPDYLIDRVDLDIRLDQQATRVVSRLSIR
                     PNPKGSADADLVLDGDGLKIKRLALDGANLDINANFVTPDRLTIASPPDEPFTLEIET
                     ELNPSANTQLMGLYRTGLAYCSQCEAEGFRRITYFLDRPDVLSVYSVRLDAEISEAPV
                     LLSNGNMTAAGVIEGTARHFAVWHDPFPKPCYLFAAVGGDLGAIHDTFVTASGREVKL
                     GIYVEHGKEASATYAMDALKRSMRWDEEAFGREYDLDVFNIVAVSDFNIGAMENKGLN
                     VFNDKYVLASPETATDGDYAQIEAVIAHEYFHNWTGNRITCRDWFQLCLKEGLTVFRD
                     HEFSADMRSRAVRRIADVRTLRAQQFPEDAGPLAHNVRPETYYEINNFYTATVYEKGA
                     EIIRMLKVLIGAQTFRAGMDLYFTRYDGTAATVEQFIACFAEVSGRDMTQFFRWYTQA
                     GTPLVEVTANYDPATQALTLDLVQSSKPTPSQDEKLPFVIPIELGLLSRDGKIFDLVT
                     GPQNGASADELIGGLIELTTPQRRVIFHALPEQPVVSLLRGFSAPVSLAYAASEDDLI
                     TQTAHDSDSFNRWQASQTYATRLLLRSVEQVRAGGGPVSDARFIEALRHVLKTSAVDP
                     AFAAQVVTLPGEADIAREIGNHVDPDAIFVARKALRQAIGSSLTAELRAIYDSLADDG
                     AYSPDSVAAGRRALRNAALDLYAAANPEGITLAGTQFTRARFMSDKITALNIMALQPG
                     AAREAALQAYYETYRNEPLAIDKWFALQAAIPESG"
BASE COUNT         1265 a         2125 c         1980 g         1422 t
ORIGIN      
        1 cacaacgaat atttgaaatg agcagcctgc gtcggtacag cgcaggtaag atgcgccgaa
       61 ctttgtcttt gtcgagtcaa tcttcgctta cagggctgga atttttgagc ggctttgtgt
      121 gcgccgcggt gtaatgataa tgatctcgca agacgcagcg cgaaattgca ttaaatattt
      181 atatcttgtc tggagcgatt atcatcttgg aagaccacgg tcgcacaatt cgagcgagca
      241 acgacgccga gcgcgtttgc ggcaacccgc aggcatccgt cgggttccgg cttgcgaaaa
      301 atatttctat ccggtcttga aatggcagcg acgcaatgac ggaagacaaa cttcgccacg
      361 ctctggttga tgcctctcgc gagctggaaa aactccaaat caatcagggc acctccggca
      421 atctgtctgc ccggtccggc gacgcgatgc tgatcacgcc aagcggcatt ccctatgaca
      481 aactgacgcc tgagatgatc gccctcatgc caatgtcggt aaacgacggc gcctggcggg
      541 gcccgcaaaa gccttcgagc gaatggcgta tgcatctcga cattctctgt gcccgtcccg
      601 atgacggtgc cgtgatccat acgcattcgg tatacgcgac agccctttcg atgctgcgcg
      661 aagaaattcc cgctgcgcat tatatggttg ccgccttcgg cggtcccaat gtgcgctgca
      721 caccttacgc accttatggc acgcaggagc tttccgatct cgtcattgag ggcttacgcg
      781 atcgtcacgg tgttttactt ggccagcacg ggatggttgt gacgggccgc gatcttggcc
      841 aagcgatgtg gcgtgccgtc gaacttgaaa cactagcgaa aatgtatgtg atcgcccgca
      901 gcatcggccg gccgcacatt ctgcccagcg aagagattgc gataacggtc gagcgcttca
      961 agtcttacgg ctatcagtcc gatgcagtcg cggcgcttgt tgcagcaccg cggacgtcgc
     1021 cgtcaaaaaa atccgccggc aaaccgaaga agctctgatt taccagacgc cggtgttgga
     1081 catcgaatgc cacggctctt gcgcgggctt cgcaccgcct ttttgcaaga gttcgatcga
     1141 gatattgtcc ggcgaacgga tgaaagccat atgcccgtcc cgcggcggcc gattgatcgt
     1201 cacgcccgcg ttctgcaatt tttcgcaggt cgcataaata tcatcgaccg cgaacgccag
     1261 atggccgaaa ttgcgcccgc ctgtgtaagt ttccgggtcc caattatagg tgagctcgat
     1321 gagcggccgc ttcgtcgtct tggccgcctc cgcatcgccg ggcgcggcga gataaaccaa
     1381 agtgaagcgg cccttctcgt taacgatccg gctcatttcg acgaggccaa acttgtcgca
     1441 ataaaaatcg agagactggt cgaggtccgt gacgcggacc atggtgtgga gatattccat
     1501 ttgcgcacct gctgagggct agaagccttg ccgttcgcgg gtcgaggctg ctagaacccg
     1561 cccgccgccg cggctttatc tctcattagg cgccggacgc gcaatgggcc caggctcaac
     1621 ggatcaagga aagagcccgc cgtcgcctcc atcgcggcga gcgcgccgct cgccgtcgcc
     1681 aaatatgcca ctatcctgat gagatagctt tcgcggaaga cgctcgtgat cgaaaataac
     1741 tacataaaag agcgggccgc cctcgtttca gttggcgtga aaactcttct tgccatcgcc
     1801 aagcttgtcg ccggccttat gtcaggctcg cttgccttgc tgtcggaagc ggccaacaac
     1861 atcggcgata tcgccatcgt ttgtttcagc ttcgtcgcca tccgcattgc caacaagccg
     1921 gctgacgaca cgcaccatta tggccatgcc aaggtcgaga cgctcgcggc tttggcgcaa
     1981 acgggatttt tgctggggct ggcggtctat attttcgcga ctgccgtcaa tcggctcatg
     2041 attggatacg tcaatgtcgt tcctggcgct ttatccttcg gggttttggt cgtctcgatt
     2101 atcgtcgata tttcgcgttg gtattcactt aacaagatcg ccaaaaagac caggagcgag
     2161 gcccttgcgg ccgacgcttt gaatttcgca agtgacatcg tcgcatcgac ttttgctcta
     2221 cttggacttc ttgccgttca tttcggtttt tggcagggcg attcgatcgg cgcacttggg
     2281 gtcgccgtgt tcatcgctat tgccggtttc aatctcgcca ggcgcacaat aaatgcctta
     2341 acggatgccg cgccgcccgg cctcactgaa agtatcagcg cggcggcgct gcgtgtgccc
     2401 ggcgtcatcg ccgtcgatag cctgcggctg cgtccggtcg gccccaacgt gctcggcgat
     2461 atctccatcc gcgtaccccg tacattgccg caggacggcg tgacggccat caagggcaat
     2521 gtcagcgccg ccatcgcagc aacccatcct gatgtggaac tgacggtcga gacgacgccg
     2581 gtcgcactcg acgacgagag cgtcgtggag cggctgctcc tcattgcagc gaagcggcac
     2641 gtcgccattc atcacgtcat tgtccagcag gtctgcggca agatcgcgct ttcctgcgat
     2701 atcgaagtcg atgggacgat gccgcttggc caggcgcata gtatcgcttc aggccttgag
     2761 gccgaggcac gcaaggagct tggcccggac atcgagatcg acacgcatat cgaaccgctg
     2821 gagccccgcg aactcgcggg cgaagatgca ccgggagaaa cgcgtgtcgc gatcgccaat
     2881 gcgctgtccg ccgccgccag cgacggcatc gaagacattc acaacgtccg ggcccgcaag
     2941 acggcgtcgg gactcgtcgt caactaccat tgccgcgccg atccgcgcgc cagcgtcgcc
     3001 gcggttcacc aagccgtgga tgcgctcgaa catagggtgc gcgaagaatt tccggcgatc
     3061 gcgcggctcg tcggccacgc cgaacctttg cgctgaggcc ctgtgttcct ttcccttctc
     3121 cgcagccggc gatttgcggg cctgttctgg tgccagttcc tttcggcctt caacgacaat
     3181 ttcgttcgta atcttctggt gacgctgatc ctgttcaggc tcggccgaga gaatgccggc
     3241 gcgctgatca cgctggcggt cggtgttttc atcctgccat ctgtgttcct ctcgggcctt
     3301 gccggcgaac tggccgacgc gcatgacaag gccttggtcg cgcgccgcct gaagatcggc
     3361 gaaatcttcg tacaggccat cgcggcggcg ggggtatggt ttggctcgtt gccacttctt
     3421 tatgtcgcgc tctgcgggct cggaaccatt gcggcgctct tcgggccggt gaaatacggg
     3481 tttctgccgg aacatctgga gacccgggaa ttgcccgcag gaaatgcctt ggtcgaagct
     3541 gcgacctttc tcgcgattct cctcggtctc gtcgccggcg cgcttgcggc agacaagacg
     3601 ctcgcgccgg gcacgatcgt cgttcagctt atgctcgtcg cggctgcggc ctatgcgacg
     3661 agccttttca tcccgcgcaa aagcgccgct gcacccgatc tgccagttca ccgtaatatt
     3721 ctcgcctcga cggtcgaact cgtgcgcgaa ttacaggccg aaccgaaact ctggcggccc
     3781 gggctcgcgg tttcgtggtt ttggctgacg ggcgccgtcg cgttgtcgct ggtgccggtg
     3841 gcggttcgca acgcgaccgg cggcggcatc gccgtcgagg ctgccatcag cggctttttc
     3901 gccttcggca ttggtgcggg gtcgattgca gcagcactcc tcgcaagagg ccgtatcagc
     3961 ctcgccccgg cgccagcggc cgccatcgcc atggctgcct ttctcctagc cttggcttgg
     4021 tccacgcgtg gccttccggc gccacacacc gcagacacaa gtctcggtag cttcttcgcg
     4081 accgcacgcg gcctgatgat cgctgtggaa gtttttggct tggcggcagc aggtggtctc
     4141 tttgtcgtgc cgttgttctc cgcgatccaa gcccgggcgg cgccagatag acgcgcccgc
     4201 acggtcgcgg cggtgaacat tcagaacgcc cttttcatgg tcgcgggctc gcttttgacc
     4261 gcatcgctgc aaagccgttt ctttggcctt ggtgagccca cgctgctggc gctcctcggt
     4321 ttcggcaatg tcgcggccgc cgcctatatc cgccgcgcca tcatcggcgc cgaggcaaaa
     4381 tctggttgac cgtcacgcgt ttgcgcctcg caccggaatc tctatgatcc tcacaacgtt
     4441 gagattccgt cccgttgcga ggcccagatg cgtaccgatg tcgcccaacc gatccgcttg
     4501 gcggactatc gcgcaccgga ctatctgatc gaccgcgtag acctcgacat tcggcttgac
     4561 cagcaagcaa cgcgcgtcgt ctcccggctc agcataaggc ccaatccgaa gggaagcgcg
     4621 gatgcggatc tcgtgctcga cggcgacggg ctgaagatca agcgcctggc gctcgacggc
     4681 gccaacctcg acatcaatgc caatttcgtc acgcccgacc ggctgaccat cgcatcgccg
     4741 ccggacgaac cgttcacgct tgagatcgag acggagctta acccttccgc caacacacag
     4801 ctcatggggc tttaccgcac cgggctggcc tattgcagcc agtgcgaggc ggagggcttt
     4861 cgccgcataa cttatttcct cgaccggccg gacgtgctga gcgtctattc ggtccggctc
     4921 gacgctgaaa tcagcgaagc gccggttttg ctctcgaacg gcaacatgac cgcggccggc
     4981 gtgatcgaag gcacagcccg ccattttgca gtctggcacg acccgtttcc caagccctgc
     5041 tatctctttg cggcggtggg cggcgatctc ggtgcgatcc acgacacctt cgtcacggcg
     5101 tctggccgcg aggtgaagct cggcatctat gtcgagcacg gcaaggaggc cagcgccacc
     5161 tatgcgatgg acgcgctgaa acgctccatg cgctgggacg aagaggcttt cggccgcgaa
     5221 tatgatctcg atgtcttcaa catcgtcgcc gtgtccgact tcaacattgg cgcgatggag
     5281 aacaagggcc tgaatgtctt caacgacaaa tatgtgctgg catcgcccga aaccgcgacc
     5341 gacggcgatt acgcgcagat cgaagccgtc atcgcgcacg aatatttcca taactggacc
     5401 ggcaaccgca tcacctgccg cgactggttt cagctatgcc tgaaggaagg cctcaccgtc
     5461 ttccgcgacc acgaattctc ggccgacatg cgctcgcgcg cggtgcgccg cattgccgat
     5521 gtacgaacct tgcgggcgca gcaattcccc gaggatgccg gccctctcgc gcataatgtc
     5581 cggcccgaaa cctattatga gatcaacaat ttctacacgg cgacggttta cgagaagggc
     5641 gccgaaatca tccgcatgtt gaaggtgctg atcggtgcgc agacgtttcg cgccgggatg
     5701 gacctttatt ttacacgtta tgatgggact gcggcgaccg tcgaacaatt tattgcctgc
     5761 ttcgccgaag tttccgggcg ggatatgact cagttttttc gttggtatac gcaggccggt
     5821 acgccactcg tcgaagtgac cgccaattac gatccagcga cgcaggcgct gacgctcgat
     5881 cttgtgcaaa gcagcaagcc cacgccaagc caggatgaaa agctgccgtt cgtcatcccg
     5941 atcgaactcg gccttttgag ccgcgacggc aaaatcttcg acctcgtaac ggggccgcag
     6001 aacggggcga gcgccgacga acttatcgga ggcttgatcg aattgacgac gccgcagcgg
     6061 cgcgtcattt tccatgcact tccggaacag cccgtcgtct ctttgctgcg cggcttttcc
     6121 gcgccggtga gcctcgccta tgcggcaagc gaagatgacc tcattacgca gacggcgcat
     6181 gacagcgaca gcttcaatcg ctggcaggct tcgcagacct atgccacccg gctgcttctg
     6241 cgctctgtcg agcaggttcg cgccgggggc ggaccggtct cggacgcgcg tttcattgaa
     6301 gccttacgcc atgttttgaa aacgagtgct gtcgatcccg cctttgccgc gcaagtcgtt
     6361 acgctgccgg gcgaagccga cattgcccgc gagatcggca accacgtcga tcccgatgcg
     6421 atcttcgtgg cgcggaaagc gttgcgccaa gcgatcggta gcagcctcac cgcggaactg
     6481 cgggcgatct atgattcgct cgccgacgat ggggcctatt cgcccgattc tgtcgcggcc
     6541 gggcgccgcg ctttgcgcaa tgcagcactc gatctttacg cggccgcaaa tccggagggc
     6601 attacgctcg ccggaacgca gttcacgcgc gcacgcttca tgagcgacaa aatcaccgcg
     6661 ctcaacatca tggcgctcca acctggcgcg gcgcgcgaag cggccttgca agcctattac
     6721 gagacttacc ggaacgagcc gctcgccatc gataagtggt tcgccctgca agccgccatt
     6781 ccggaatccg gc
//
LOCUS       DASWOG010000130         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_131477,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000130 DASWOG010000000
VERSION     DASWOG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131477"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VGB93_02660"
     CDS             complement(<1..1128)
                     /locus_tag="VGB93_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY0145063.1"
                     /translation="MIVRRFMNWARTASAAQRAEAAAALARVYLITDLEPSELEEADL
                     ALTGLLDDASPLVRRALAEAFASAPDAPHHIVLGLADDQSDISSIVLGRSPVLSDSDL
                     IDCAAIGDAIAQSAIALRPVVSAAVAAAIAEVGAREALISLAINPGADLPAFSLRRMV
                     ERFGFDAEMREALLMRPGLPTALRADLVSAMADALRAFVTEREWMSGERAQRVMRDAC
                     EQARVIIAATSEEGGGAVALIAHLRTCGQLTASLLLRALLSGNVSLLEAALAELSGLP
                     IMRVKGLTRDWRGAGFAALYRKAGMPDALMPAFRAAIEALGNCDVGDATGASLSRQRV
                     ERVLTACKAENAGELDSLLAVLRRFAAEAARDEARQFSAALP"
     gene            1219..1860
                     /locus_tag="VGB93_02665"
     CDS             1219..1860
                     /locus_tag="VGB93_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0145064.1"
                     /translation="MRVHLFVPSDPATLDRAASSGADALWLKGPIGADALRAAQQRAA
                     NPKLYVCLGALNGAFDKDLDTAMAAAPDGIVLPTHNGTDVQHLGVKLAVREAELGLAD
                     GTTQIIALVSAPGAIFQLGSFVGASPRLVALAFDAQAFAASLGVADLSAPPLGLARDL
                     TLLAAKAAGVAAILADDAAANSPEACKAAKRDGFDAILARDPADIATVRASLK"
     gene            complement(1866..>2094)
                     /locus_tag="VGB93_02670"
     CDS             complement(1866..>2094)
                     /locus_tag="VGB93_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0145065.1"
                     /translation="ALECKVIDILLLKDLDGALTGNVLVFGQVVGVYVDDAFIKEGRV
                     DTSAMRPIARCGYMDYAVTDELFEMPRPRRV"
BASE COUNT          421 a          683 c          642 g          348 t
ORIGIN      
        1 tggcagagcc gcagagaatt gtctggcttc atctcgcgcg gcttcagccg cgaagcgacg
       61 tagaaccgca agcagcgaat cgagttcgcc ggcgttttcg gccttgcagg ccgtcaacac
      121 acgctcgacc ctctggcgtg aaagactcgc acccgtcgcg tcgcccacgt cgcaattgcc
      181 aagcgcttcg atcgccgcgc ggaaggctgg catcagggca tccggcattc cggctttccg
      241 gtaaagcgcg gcgaagcccg caccgcgcca atcgcgcgtc aggcctttca cccgcatgat
      301 cggcaagccg gaaagttcgg cgagtgccgc ttcaagcagg ctgacattac cggagagcag
      361 cgcgcgcaga agcaggctcg cagtgagctg tccgcaggtg cgcaagtggg cgatgagcgc
      421 gacggcgcca ccgccctcct cgctcgtcgc agcaatgatg acccgcgcct gttcgcaagc
      481 atcgcgcatc acacgttgcg cccgttcgcc agacatccat tcccgttcgg tcacgaaagc
      541 ccgcagggca tccgccatgg cggagacgag gtcggctcgc aaggcggtcg gcagaccggg
      601 ccgcatcagc aaggcttcgc gcatttcggc atcgaaaccg aaccgctcga ccatgcggcg
      661 tagcgaaaac gccggcagat cggcgccggg attgatggca agcgagatca gcgcctcgcg
      721 ggcgccgact tcggcaattg cagccgcaac ggccgccgaa acgacgggac gcagcgcaat
      781 cgcggattgg gcaatcgcat cgccgatcgc cgcgcaatcg atgagatcgg aatcggacag
      841 aaccggggag cggcccagaa cgatggagga aatatcggat tggtcgtcgg caaggccgag
      901 aacgatatga tgcggcgcat ctggcgcgct ggcaaaggcc tcggcgagcg cccggcgcac
      961 cagcggcgaa gcgtcatcga gcagcccggt cagggccaga tcagcttctt ccagctcgga
     1021 gggctccaga tcggtgatga gatagacgcg tgccagggcc gctgccgctt ccgcccgctg
     1081 tgctgcgctg gcggttcgcg cccaattcat aaatcgacgc acaatcatag gaatgtcctg
     1141 cacgcagcgg caaacgcagc gtcttaagtt ctcattcacc atccgcgatc atggtaaacg
     1201 tacccttaag gtagagacat gcgcgttcat cttttcgttc cgtccgaccc ggcaacgctc
     1261 gacagggcgg cttcgagcgg cgcggatgca ctttggctta aaggcccgat tggagcggac
     1321 gccctacgcg cggcgcagca gcgagcggcg aatccgaagc tctacgtctg cctcggcgct
     1381 ctcaatggcg ctttcgacaa agatctcgat acggcgatgg ccgccgcgcc cgacggaatc
     1441 gttctgccga cccacaatgg cacggatgtg cagcatctcg gcgtgaagct ggccgtccgc
     1501 gaagccgaat tgggacttgc cgacggcaca acccagatca ttgcgcttgt ctctgcgcct
     1561 ggcgctattt ttcagttagg cagcttcgtg ggtgcaagcc cgcggctcgt tgcgctcgcc
     1621 ttcgacgcgc aggccttcgc agcatcgctg ggcgtcgccg atttgagcgc accgccgctc
     1681 ggcctcgcgc gcgacctcac gcttctcgcg gcaaaagccg ccggcgtcgc ggccattctc
     1741 gcggacgatg cagcagcaaa ttcacccgag gcctgcaaag cggcgaagcg cgacggcttc
     1801 gacgcgatac ttgcgcgcga tcccgccgac attgcgaccg tgcgcgctag tctcaaatag
     1861 agtcttcaga cacgacgcgg ccgcggcatt tcaaacaatt cgtcggtgac ggcgtagtcc
     1921 atatagccgc aacgtgcgat cggacgcatg gccgacgtat cgacccgtcc ttctttgatg
     1981 aaagcatcgt cgacatagac gccgacgacc tgaccgaaaa cgagaacatt gccagtcaac
     2041 gcgccgtcca aatcctttaa gagcagaata tcaatgacct tacattcgag tgca
//
LOCUS       DASWOG010000131         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_167969,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000131 DASWOG010000000
VERSION     DASWOG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167969"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3032)
                     /gene="polA"
                     /locus_tag="VGB93_02675"
     CDS             complement(<1..3032)
                     /gene="polA"
                     /locus_tag="VGB93_02675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0145066.1"
                     /translation="MSLHNRPVKAGDHVFLVDGSSFIFRAYFQSIRQDAKYNYRSDQL
                     PTGAVRLFCNKLFQFVREGAAGIMPTHLGIIFDKSENSFRRELYPPYKANRSEPPEDL
                     IPQFPLMRAAVRAFGLVPIEQDRYEADDLIATYACQARERGADVLIISADKDLMQLIR
                     DGIAMYDPASGEAGAKGAREERRIGLAEVQDYFGVPPEKVIDVQALAGDSTDNVPGAR
                     GIGVKTAAQLINEYGDLDTLLARAGEIKQPKRREILTAPESVDLIKVSKQLVTLVRDV
                     ALDVPLDDLGLEQPEGKTLVGFLKAMEFTTITKRAAETYGVVAEAIEADAAFLGPAGW
                     RGRNGDIVETVEAIEEAEEKIEEAKPTPTGPRRNARYGETPQRQIAPSGPAELAAARA
                     ALALVSSFDRSTYKTITDFAALDAVIAAAFDAGFVAIDTETSSLDPMQAELIGVSLCV
                     KEGEGVYVPLRHRGEGAGDLFGGDALLPGQLPDDDVLARLKPLLESESVLKIAHNSKF
                     DTLVFRQHGIAMRPIDDTLLLSYVLDAGLTDHGMDVLSEKFFHHKPLAFGEVAGSGRT
                     FIGFARVPIDKATEYAAEDADVTLRLWNVLKPRLPAERMTSVYEALERPMVEVLARME
                     ARGISIDRAILSRLSGEFAQDMARLEAEIFELAGESFNLGSPKQLGDILFGKMGLSGA
                     RKTATGAWSTAAGVLEDLAEEGNQFAGRILDWRQLSKLKSTYTDALPGYVNPRTHRVH
                     TSYALAATTTGRLSSSEPNLQNIPIRTEAGRKIRRAFIAPPGHKLISADYSQIELRLL
                     AHIANIPQLRQAFADGLDIHAMTASEMFGVPVEGMLSEVRRRAKAINFGIIYGISAFG
                     LANQLGIPRDEAGAYIRQYFERFPGIRAYIDQTKNIARAQGYVTTLFGRKCHYPRINA
                     SNPSERAFNERASINAPIQGSAADIIRRAMVRMEAALDKEGLSAQMLLQVHDELVFEV
                     PDAEVEATIALVRRVMVDAPHPAVQLSVPLQVEAKAAQ"
     gene            3332..>4124
                     /locus_tag="VGB93_02680"
     CDS             3332..>4124
                     /locus_tag="VGB93_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0145067.1"
                     /translation="MNLLESVEQTLGTALGRNSAAWPPSHLSLALQGGGSFGAFTWGV
                     LDRLLESGDVDIDTVSGTSAGAVNAVLLADGLAAGGRDGAREKLTHFWERLSRNAAFG
                     PFGSTAHFAPGGAAGTFSAWARLLSPYQYNPLDVNPLRTLLNEEIDFERLKASSPIRL
                     LIAATRVSDGRARIFDNAEINAEVVLASSCLPLFHHAVTIDDIAYWDGGYSANPPLVD
                     LVSASQATDLLVVRITPTLSAETPTSSREILRRLDQITFNASLQTE"
BASE COUNT          795 a         1273 c         1242 g          814 t
ORIGIN      
        1 ttctgcgccg ccttggcctc gacttgcaga ggcacagaga gttgcaccgc cggatgcggc
       61 gcatccacca tcactctgcg aaccagcgcg atcgtcgcct cgacctcggc gtccggcacc
      121 tcaaaaacga gttcgtcatg cacttgcaga agcatttgcg cggaaagccc ctctttatcc
      181 aacgccgcct ccatccgcac catcgcgcgg cggatgatgt cggcagcaga cccctggatg
      241 ggcgcattga tcgacgcgcg ctcattgaag gcgcgttcag aagggttcga cgcattgatg
      301 cgcggataat ggcatttgcg tccgaagagc gtcgtcacat agccctgcgc gcgcgcaata
      361 ttcttcgtct gatcgatata ggctcgaatg ccgggaaaac gctcgaaata ttgtcggata
      421 taagcacccg cctcatcgcg cggaatgccg agctggttgg cgaggccgaa tgccgaaatg
      481 ccgtagatga tgccgaaatt gatcgccttg gcgcggcggc gcacttcgga tagcatacct
      541 tcgaccggca cgccgaacat ttccgacgcc gtcatcgcgt gaatgtcgag tccatcggcg
      601 aacgcctgac gcaattgcgg aatattggca atatgcgcga gaagccgcaa ttcgatctgc
      661 gaataatccg ccgagataag cttgtgcccc ggcggcgcga taaaggcgcg gcggattttg
      721 cgtcccgcct cggtgcggat cgggatattc tgcaaattcg gctcggacga cgagagtcgg
      781 cccgtcgtcg tcgcagcgag cgcgtaggag gtatgaacgc gatgcgtgcg cggattgacg
      841 taccccggca gggcgtcggt gtaagttgac ttgagcttcg agagttgccg ccagtcgaga
      901 atgcgcccgg cgaattgatt gccttcctcg gccaaatctt caagcacgcc tgccgccgtt
      961 gaccaggcgc cggtcgccgt cttccgcgcg ccggaaaggc ccatcttgcc gaagagaata
     1021 tcgcccaatt gtttcggcga gccgaggttg aagctttcac cggcaagctc gaatatctcg
     1081 gcttcgagcc gcgccatgtc ctgcgcgaac tcgccggaaa gacgcgagag aatcgcccga
     1141 tcgatggaaa tgccgcgcgc ttccatccgc gccagcacct cgaccatcgg ccgctccagc
     1201 gcctcataga cgctggtcat gcgctcggcc ggcaggcggg gcttcagcac gttccaaagc
     1261 cgtaaggtca catcggcgtc ttccgccgca tattcggttg ccttgtcgat cggcacacgc
     1321 gcgaaaccga tgaatgtgcg gcccgagcct gcgacttcgc cgaacgccag cggcttatgg
     1381 tgaaagaact tctcgctcag cacatccatg ccgtgatcgg tcaggcccgc gtcgagcaca
     1441 taggagagta gcaacgtgtc gtcgatgggc cgcatcgcga tgccgtgctg cctgaacacg
     1501 agcgtatcga atttggaatt atgcgcgatc ttcaagacgc tttcggattc gagcagcggc
     1561 tttagacgcg ccagcacatc gtcgtcgggc aactgacccg gcagcagcgc gtcgccgcca
     1621 aacagatcgc ccgcgccctc accacgatgg cgcaacggca cgtagacgcc ctccccctcc
     1681 ttaacgcaga gcgaaacgcc gataagctcg gcctgcatcg gatcgagcga tgacgtctca
     1741 gtgtcgatcg cgacaaatcc ggcatcgaaa gccgcggcaa tgacggcatc gagcgcagcg
     1801 aaatccgtga tcgtcttata ggtgctgcgg tcaaacgatg agacaagcgc cagcgcagcg
     1861 cgcgcggcag caagttccgc cggtcccgac ggcgcgatct ggcgttgcgg tgtctcaccg
     1921 tagcgcgcat tcctgcgcgg gccggtcggc gtgggcttgg cttcctcgat cttctcttcg
     1981 gcttcttcga ttgcttcaac ggtttcgacg atgtcgccat tgcggccgcg ccagccagcc
     2041 ggcccgagga atgcagcgtc cgcctcgata gcttccgcca cgacgccata ggtctcggcg
     2101 gcgcgtttgg tgatcgtcgt gaattccatc gccttgagga aaccgacgag cgtcttgccc
     2161 tccggctgct ccagaccgag atcgtcgagc ggcacgtcga gcgcgacgtc gcggacaagt
     2221 gtgacgagct gtttcgaaac cttgatgaga tcgacactct ccggtgcggt gaggatttca
     2281 cggcgcttgg gctgtttgat ctcgccagca cgcgccagca atgtatcaag atcgccatat
     2341 tcattgatga gttgcgccgc ggtcttgacg ccgatgccgc gcgcgcccgg cacattgtcg
     2401 gtcgaatcgc cggcgagtgc ctgaacatcg atcacctttt ccggcggcac gccgaaataa
     2461 tcctgcactt cggcgaggcc gatgcgtcgc tcctcccgcg cacccttggc gcctgcttcg
     2521 ccggatgccg gatcgtacat ggcgattccg tcgcggatga gctgcatcaa atctttatcg
     2581 gccgagatga tcagcacgtc ggcgccgcgc tcgcgcgcct ggcacgcata ggtcgcgatg
     2641 agatcgtcgg cttcgtatcg gtcctgttcg atcggcacga ggccgaaggc acggacagcc
     2701 gcccgcatta gcggaaattg cggaatcaga tcttccggcg gctccgaacg attggccttg
     2761 tagggcggat aaagttcgcg gcggaacgag ttttccgatt tgtcgaaaat gatgccgaga
     2821 tgcgtcggca tgattcccgc cgcgccctcg cggacgaact ggaaaagctt gttgcagaac
     2881 aaacgcaccg cgccggtcgg caattgatcc gaccggtaat tgtatttcgc gtcctgccga
     2941 atcgactgga aataggcgcg gaaaatgaac gacgacccgt cgacgaggaa gacatggtct
     3001 ccggctttca ccgggcgatt gtgcagcgac atgatgtgag aagggctcaa aaaagggtcc
     3061 gcgtgtggct aacataatcg gcaagttgaa gcgatgccac gccgccccgg tgtggaactt
     3121 aactaaaccg cgtcgatttc aaaggcttat gctgcattgc accgccacgt gacggattgg
     3181 agccttttcg tcacactgtg cgttacataa cactatgaga tttccacata aatcgtcttt
     3241 tgcgcgccat tcgcaatcca tattgcgatg cactatatca gggcccaaca tggtcggcgc
     3301 atgacgccgg caaggaattt cggagtgcgg aatgaactta ttggaaagcg tcgagcagac
     3361 gctgggcaca gcacttggcc gtaatagtgc ggcttggccg ccatcccact tgtcgctggc
     3421 tttgcaaggg ggtggatcgt tcggcgcctt cacgtggggc gtgctcgatc gcctgctcga
     3481 atcaggcgat gtcgatattg ataccgtcag tggcacgtcc gctggcgcgg tgaacgctgt
     3541 gctgctggcg gacggcctcg cagcgggtgg tcgcgatggc gcgcgggaga agctcacaca
     3601 tttctgggag cggctgagcc gcaatgccgc cttcgggccc ttcggatcga cggcgcattt
     3661 cgcgcccggc ggcgcggccg gcaccttctc ggcctgggcg cggctcctct caccctatca
     3721 atacaacccg ctcgacgtga atccactgcg cacgctcctt aacgaggaga tcgattttga
     3781 gcggctgaaa gcttcctcgc caatccgcct gctgattgcg gcgacgcgtg tctcggatgg
     3841 ccgggcgcgc attttcgaca atgccgagat caacgctgaa gttgtgctcg cctcgtcctg
     3901 tctaccgctc ttccatcatg cggtgacgat cgacgacatt gcgtattggg acgggggtta
     3961 ttccgccaac ccgccgcttg tcgatctcgt ttccgcgagc caggcgacag acctgctggt
     4021 cgtgcgcatc acgcccacgc taagcgccga aacgccgaca agctcgcgcg aaatcctgcg
     4081 ccggcttgat cagatcacgt tcaacgcctc gctgcagacc gagt
//
LOCUS       DASWOG010000132         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_94975,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000132 DASWOG010000000
VERSION     DASWOG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94975"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VGB93_02685"
     CDS             <1..596
                     /locus_tag="VGB93_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0145068.1"
                     /translation="PPAATPPFKPMDPHVAIQKADDYFNSTRSMIGDFVQISGDRRSE
                     GKVYIQKPGKMRFEYAEPATLDIVADGMTVAVIDHKLATQDYYFIWQTPLKFLLKDRI
                     DLLRDLAVTDVVSDPDNVTITVVDQETFGGTSRIKLMFDPASFQLKQWEVTDPQGNET
                     LVSLFNVDRSTPPDPSVFQIKSSRLDSSNSRLLNRSN"
     gene            890..1705
                     /locus_tag="VGB93_02690"
     CDS             890..1705
                     /locus_tag="VGB93_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0145069.1"
                     /translation="MPFTVATWNINSVRLRIDLVGKFLSDHAPDVLCLQETKCRDAEF
                     PAAAFRRFGYEHIALNGQKGYHGVAIVSKTPFGIVDRRPFCDKGDARHISVTVAPTNV
                     RAFTLHNFYVPAGGDEPDPDINPKFAHKLAFLDEMASWISADRVAGGDAIMVGDLNVA
                     PLETDVWNHKALLKVVSHTPIEVEKLDAVFAAGPWVDALRRYVPAEEKLYTWWSDRSP
                     DWAKANKGRRLDHIWTSAALAPNLRGMKVLRESRAWERPSDHVPVLAHFDFDA"
BASE COUNT          348 a          526 c          479 g          361 t
ORIGIN      
        1 cgccgccggc cgcgaccccg ccctttaagc ccatggatcc gcatgtcgcg attcagaagg
       61 ctgacgatta tttcaattcg acacggtcga tgatcggtga tttcgtgcag atcagcggtg
      121 accggcgctc ggaaggtaaa gtctacatcc agaagcccgg caaaatgcgc tttgaatatg
      181 ccgagccagc aacgcttgat atcgtcgccg acggcatgac ggtcgcggtg atcgaccaca
      241 agctcgccac gcaggattac tattttatct ggcagacgcc gttgaaattc ctgctcaagg
      301 atcggatcga tcttttgcgc gatctcgccg tgaccgatgt cgtcagcgac cccgacaacg
      361 tgacgatcac agtcgtcgat caggaaacct tcggcggcac atcgcgcatc aagctaatgt
      421 tcgacccggc gtctttccag ctcaaacaat gggaagtcac cgatccgcaa ggtaacgaaa
      481 cgctggtttc gctattcaac gtggatcgtt cgacgccgcc ggacccttcc gttttccaga
      541 tcaagtccag ccggctcgac agctcgaaca gccggctcct gaatcggagc aattaacgtt
      601 tcgcaataat ttgcggccgg acttgcagtc cgtttggccc ggacctatct cacttttcgg
      661 agagtgatac ggtcggcttg acgaattccc cccgttctgt ctcgccggct aggccggacc
      721 ccctcccggt acagtgtcgc tccacggggc gtccgatctc gatcgggcgc cctattttta
      781 tggggcgcat cgcacctgca gcaaaatcct gtgattgcgg caaggctttc cttgctgccc
      841 ggcgcaccgc gtgctcaaca acgatagctt cagatttccg ggatcaaaat tgccgtttac
      901 cgtcgccacc tggaatatca attctgttcg tttgcgtatc gatctcgtcg gcaaatttct
      961 gtcagaccat gcgccagacg ttctttgcct gcaggagacg aagtgccggg atgccgaatt
     1021 ccccgccgcc gcgttccgca ggttcggcta cgagcacatc gcgctgaacg ggcaaaaggg
     1081 ctatcacggc gtcgccatcg tctcgaaaac gccgttcggc attgtcgatc ggcggccgtt
     1141 ttgcgacaag ggcgatgcgc gccatatcag cgtgaccgtt gcgccgacga atgtccgcgc
     1201 ctttacgctg cataattttt acgtgccggc cggcggcgac gagcccgacc ccgacatcaa
     1261 tccgaaattc gcccacaaac tcgcctttct cgacgagatg gcaagctgga tcagcgccga
     1321 tcgcgttgcg ggaggtgatg ccatcatggt cggcgacctc aatgtggcgc cgctcgaaac
     1381 cgatgtctgg aatcataagg cgttgctcaa agtcgtgagc catacgccga tcgaggttga
     1441 gaagctggat gcggttttcg ctgcgggacc gtgggtcgac gcgctgcgcc gctacgtccc
     1501 agcggaggaa aagctttata cttggtggag cgatcgctca cccgattggg cgaaagcgaa
     1561 caagggccgc aggctcgacc acatctggac aagcgcggct ttggccccga acctacgcgg
     1621 catgaaagtc ttgcgcgaaa gccgcgcctg ggaacggcca tccgaccatg tccccgtcct
     1681 ggcgcatttc gatttcgacg cctgatcccg aaaa
//
LOCUS       DASWOG010000133         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_296715,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000133 DASWOG010000000
VERSION     DASWOG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296715"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGB93_02695"
     CDS             complement(<1..670)
                     /locus_tag="VGB93_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145070.1"
                     /translation="MNIANEEREAIEELLPWRAAGTLSRSETQRVDAALAADPELARR
                     YALVREEVTQTVQLNEALGTPSPRTLDALFAKIDAEPGRKPAADRANLFGRVVEFFAS
                     LSPRTLAFSTAAAALVLTLQAGVIGTYMLKDKTSGGYETASEASKPLGAGSYVLIRFA
                     PQTNADAITKFLQANKLTIANGPSAGGLYLVRATPTKISPVNLQRLADRLQHDKAVGF
                     IVPSE"
     gene            complement(667..1227)
                     /locus_tag="VGB93_02700"
     CDS             complement(667..1227)
                     /locus_tag="VGB93_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0145071.1"
                     /translation="MQTTTDEDLVGRIARGDRLAIQLLFARYQTPVYRFVLRMVRNEA
                     NAEDLISDVFLDVWRQAGKFEGRSSVSTWLLSIARFKALSALRRKPESALDEETASAI
                     EDRSDDPEITLAKKDKGAAMRACLGKLSTEHREIIDLVYYHEKSVEEVAEIVGIPTAT
                     VKTRMFYARRKLSELLNERGIDRGWP"
     gene            complement(1252..1527)
                     /locus_tag="VGB93_02705"
     CDS             complement(1252..1527)
                     /locus_tag="VGB93_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145072.1"
                     /translation="MMILRDIGSLRVCELQCQLVEAGRRKVQTSGGIRFGKRRGRALA
                     HRQPGAKLQQTMLFEGGSELIWPQGRMIARPRIFIRLRLNLHTPDGD"
     gene            1508..2299
                     /locus_tag="VGB93_02710"
     CDS             1508..2299
                     /locus_tag="VGB93_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145073.1"
                     /translation="MSRKIIITLAATIVVAASSLVSGSADASGFGGGHFGGGFGRGGF
                     TRISAGGFGGGHFGGGHFGGGHFGGRGHNGGRLVPVLNNPGRGGRGGGHGGGHGGGRG
                     PGYPFPPRHPFPSIVWHGHHHPHGHWVFRGGQWVIDDVAVASVGVAAVGAVATDPAPG
                     PCTCLFKTYTPDGKVVFSDMCTKESASAAVGTSASDAMQAPTQSSGAQMTPAEVTLMS
                     TSVDYDGRTYAAYLAANPDVAQAQAAAQAQSPQPSQPAPETPKGN"
BASE COUNT          439 a          794 c          785 g          507 t
ORIGIN      
        1 actccgaggg cacgataaag ccgacggctt tgtcgtgttg caatctgtct gcgagacgtt
       61 ggagattgac cggcgatatt ttcgtcggcg tcgcccggac gagatagaga ccgcctgcgg
      121 aagggccgtt ggcaatcgtc aatttgttgg cttgcaggaa tttagtgatc gcatcggcat
      181 tcgtctgcgg cgcgaagcgg atcaaaacgt aggaccccgc gccgagcggc ttgctcgcct
      241 ctgaagccgt ttcgtaaccg cccgacgttt tgtctttgag catataagtg ccgatgacgc
      301 cggcctgtaa ggtcaggacg agcgctgccg cagcggtact aaaggccaat gtgcgcggcg
      361 acaggctcgc aaaaaattcg acgacacggc cgaaaagatt ggcacggtct gcggccggct
      421 tgcggcccgg ttctgcatcg atcttggcga acaaggcgtc cagcgtgcgt ggcgaaggtg
      481 tgcccaaagc ctcgttgagc tggaccgttt gcgtcacttc ctcacggaca agggcgtagc
      541 ggcgcgcgag ttcagggtcc gcggcgagcg ccgcatcgac gcgctgcgtt tcgctgcggc
      601 tcagcgtacc ggcggcccgc cagggcagaa gctcttcgat cgcttcgcgc tcttcgtttg
      661 caatgttcat ggccagcctc gatcgatccc acgttcgttt aggagttccg acaatttccg
      721 acgcgcgtag aacatccgcg tttttacggt tgccgtaggg atgccgacga tctcggcgac
      781 ctcctcaacc gacttctcgt ggtagtagac aaggtcgatg atctcgcgat gctcggtcga
      841 aagcttgccg agacatgcac gcattgcagc acccttgtcc ttcttcgcca gcgtgatctc
      901 cggatcgtcg cttcgatcct cgatcgcact tgcggtttcc tcgtcgagcg cgctctccgg
      961 cttgcggcgc aaggccgaca gcgccttgaa acgcgcgatg ctcaagagcc aggtcgagac
     1021 gcttgagcga ccctcgaatt ttcctgcctg tcgccagacg tcgagaaata cgtcgctgat
     1081 caaatcctct gcattggctt cgttgcgcac catgcggaga acgaagcgat agaccggcgt
     1141 ctggtaacgc gcgaacagca attgtatcgc gagccggtcg cccctggcga ttcgcccgac
     1201 gagatcttcg tccgtcgtcg tttgcatcgg catggccatc gactcgcaat gttagtcgcc
     1261 gtcgggcgtg tgcaggttca atcgcaatcg aataaaaatt ctcggccgcg cgatcatgcg
     1321 cccctgcggc cagatcaatt ccgaaccgcc ttcaaatagc atggtttgct gaagtttagc
     1381 gccgggctgc cggtgtgcca gcgcacgtcc tcgtcgctta ccgaaccgga tgccgcccga
     1441 cgtttgaacc tttctccgtc ccgcttcgac caactggcac tgcaactcgc aaacgcggag
     1501 agaaccaatg tcacgcaaaa tcatcatcac cctcgcagcc acgatcgtcg tcgcggccag
     1561 cagccttgtt tcaggatctg ccgacgccag cggtttcggc ggcggtcatt tcggtggcgg
     1621 cttcggtcgc ggcggcttca cccgcatcag cgccggtgga tttggcgggg gccatttcgg
     1681 cggcgggcac tttggtggag gtcatttcgg cggtcgcggc cataatggtg ggcggctcgt
     1741 cccggtcctt aacaaccccg gccgtggcgg ccgcgggggt gggcacggtg gtggtcatgg
     1801 cggtggtcgc ggtccgggct atcccttccc gccgcgccat ccttttccgt caatcgtttg
     1861 gcacggccac catcacccgc atgggcattg ggtgttccgg ggcgggcaat gggtgatcga
     1921 cgatgtggcc gtcgccagcg ttggtgtggc cgcggtcggc gcagtcgcca ccgatccggc
     1981 gcccggaccc tgcacctgcc tgttcaagac ctatacgccg gatggcaagg tggtcttctc
     2041 ggatatgtgc acgaaggaat cagccagcgc agcggtcgga accagcgcct ccgatgcgat
     2101 gcaggcgccc acccaatctt cgggtgccca gatgaccccg gctgaggtaa cgctgatgtc
     2161 tacgtcggtg gactacgacg ggcgcaccta cgccgcttat ctggcggcga acccggacgt
     2221 agcgcaggcg caggctgcgg cccaggccca gtcaccgcag ccgagccagc cggctcccga
     2281 gacgccaaaa ggcaattgag tctcgctatc taggccaatc ccagataaag gccgtccgcg
     2341 cttgccgcgg gcggtcttgc tcttgagctc ccctccctct ataagcccgc acggcccgtt
     2401 tacgacaccc ctggaggcga gggccgcgtt ttcaatcaag gataaagcta tggccgagac
     2461 caaaactctc gcggccacgg cgtgcagcgt tggcggcaag ggggccgctc gcagcgttcg
     2521 tcgtg
//
LOCUS       DASWOG010000134         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_39690,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000134 DASWOG010000000
VERSION     DASWOG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39690"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGB93_02715"
     CDS             <1..370
                     /locus_tag="VGB93_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145074.1"
                     /translation="SGGMIKRAALARALALDPELVCLDEPTSGLDPIGAAEFDELIAT
                     LQKTLGLTVFMVTHDLDSLFAACDRVAALADGKVIATGPIGTMLASEHPWLRSYFHGK
                     RARQADAASRPQVRAAPPTR"
     gene            384..1436
                     /locus_tag="VGB93_02720"
     CDS             384..1436
                     /locus_tag="VGB93_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0145075.1"
                     /translation="METRAHYALIGAFALAVIAVGFFSVFWLSGGGTRPQGTHTYKVI
                     FAGSIAGLGRGSFVLFNGVRVGEVTEIDLMPQDPAHVYALISVDARVPVRTDTKAELE
                     SSGFTGVQSVALSGGNVSAPILPKPAGGGPPVIMAEHSNFQDLMVAAHSITGQASDLL
                     TKANKLLDDDAPPLNASIKNIQKFSDALAANSDGVKQFLSAMTEVGKQIGPLSTKLST
                     LADDTDHVVKAVDPAQVKSMIKDLSELSAKLNKAADQVDTVLTSLGGFFKTTDSKGAF
                     QEIAGAAKSIHILADHLDERTKELTANLNHFSSTGLRQYEGLAVDGRQTLQDLDKVIK
                     SVQENPQQFIFGKKAH"
     gene            1637..>2237
                     /locus_tag="VGB93_02725"
     CDS             1637..>2237
                     /locus_tag="VGB93_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEY0145076.1"
                     /translation="MFNLNDILQSAQGGEAINNIAQRFGLSQEQAQAAVQALIPALSA
                     GLTKAAANPGTLGSVISAVNDSTHQASFNDPSAAQSPAATQKSSDTLSQILGSNHIIQ
                     QIVQRASAETGIRPDILTQILPVVASIVIGGLATSLHSQGLGGMLGQLATAAEQGNLG
                     SVLGGGGGAAAGSSGGGLISVLTNLLGGLFGGQSSSTSAA"
BASE COUNT          453 a          709 c          659 g          416 t
ORIGIN      
        1 gtccggcggc atgatcaaac gtgccgccct cgcccgcgcg ttggcgcttg accccgagct
       61 cgtctgtctt gacgagccga cgtcgggtct cgatccgatc ggcgccgccg aattcgacga
      121 actgatcgcc acgttgcaga agacactcgg gctgacggtc ttcatggtga cgcacgacct
      181 cgacagcctt ttcgcggcat gcgaccgcgt cgccgcccta gccgatggaa aagtcatcgc
      241 gaccgggccg atcggcacca tgctggcctc ggaacatccg tggctgcgca gttattttca
      301 tggcaaacgc gcgcgccagg cggacgcggc ctcccggccg caggtgcgcg cggccccacc
      361 gacacgatga acgacgagga catatggaaa ccagggccca ttacgcgctg atcggcgcct
      421 tcgcactcgc cgtgatcgcc gtcggcttct tttccgtctt ctggctttcc ggaggcggca
      481 caaggccgca aggcacgcat acttacaaag tgatcttcgc cggctcgatc gccggcctcg
      541 gtcgcggcag ctttgtgctg ttcaacggcg tgcgcgtcgg cgaagtcacc gagatcgatc
      601 tcatgccgca ggatccggcg catgtttacg ctttgatcag cgtcgatgcg cgggtgcccg
      661 tacgcactga cacgaaggcc gagcttgaat cgagcggctt caccggcgtg caatcggtgg
      721 ctctttcagg cggcaacgtc agcgcgccaa tcctgccgaa gccggccggc ggcggcccgc
      781 ccgtcatcat ggcggaacat tcgaactttc aagacttgat ggtggccgcg cattcgatca
      841 ccggccaggc ctccgatctc ttaaccaagg caaacaaact cctcgacgac gacgcgccgc
      901 ccttgaatgc gtcgatcaag aacatacaaa agttttccga cgcgctcgcc gcaaattcgg
      961 acggcgtgaa gcaattcctt tcggcgatga cggaggtcgg caagcaaatc ggaccgctct
     1021 cgacgaagct ctcgaccttg gcggacgata ccgatcatgt cgtgaaagcg gtcgatccgg
     1081 cgcaagtgaa gtcgatgatc aaggatctct cggaactctc cgccaaactc aataaggccg
     1141 ccgatcaagt cgatactgtg ctgaccagcc tcggcggctt cttcaaaacg accgattcaa
     1201 agggcgcgtt ccaggagatc gccggcgcgg ccaaatcgat ccatatcctc gccgaccatc
     1261 tcgatgagcg gacgaaagag ctgaccgcca atctcaatca tttcagcagt actggattgc
     1321 gccaatacga ggggcttgcc gtcgatgggc gtcagacgct gcaagacctc gacaaggtca
     1381 tcaaatccgt ccaggaaaat ccgcagcaat tcatcttcgg taaaaaggcg cattagaccg
     1441 ccatctcgaa agcggcgtaa gatcaatgtc ataacaggct ggtagggcgt cctcgcgcaa
     1501 tgttgcgggt tgccgggaca atggccaggg gtcgcctctc gcaaattcgc cttcaaccac
     1561 gtaggattac cgcgcgtgat acgggcgccg gcgcagcgac cgcaacaatg tcggccggca
     1621 gcgaggagat ttgtgcatgt tcaacctaaa tgacattctg caaagtgcgc agggtggcga
     1681 agccatcaat aatattgcgc agcggttcgg cctttcacag gaacaggcgc aagccgccgt
     1741 tcaggctctc atccctgctc tttcggctgg cttgacgaaa gccgcggcga accccggcac
     1801 gctcggctcg gtcatttcgg cggtcaacga cagcacccac caggcctcgt tcaatgaccc
     1861 cagcgccgcg caatcgcccg cagcgacgca gaagagcagc gatacgctgt cgcaaattct
     1921 cggttcaaat catatcatcc agcagatcgt tcaacgcgcc tcggctgaga ccggaattcg
     1981 ccccgacatc ctcactcaga ttctgccggt ggtcgcttcc atcgtcattg gtggcttggc
     2041 gacctcgctc catagtcagg gcctcggcgg aatgctgggc caactcgcaa cggctgccga
     2101 acaaggcaat ctcggctcgg tgcttggcgg cgggggcggt gcggcggcgg gaagctctgg
     2161 cggcggcctg ataagtgtat tgacgaattt gctcggtggt ctcttcggcg ggcagtcttc
     2221 gagcaccagc gccgcgg
//
LOCUS       DASWOG010000135         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_149759,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000135 DASWOG010000000
VERSION     DASWOG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149759"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..699
                     /locus_tag="VGB93_02730"
                     /pseudo
     CDS             1..699
                     /locus_tag="VGB93_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931931.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
     gene            720..3251
                     /locus_tag="VGB93_02735"
     CDS             720..3251
                     /locus_tag="VGB93_02735"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0145077.1"
                     /translation="MPAELLPLYEQLVKAPALSDPVVAEARLAALCSSSAAVTTLADV
                     PAVAALLKGIADHSPYLWKLAIAEPARLARLLTAAPEMSLADCLIDLRESLTGANEAG
                     AMRLLRRAKQDVALLVALADLGGLWTTRDVMSALTRAADLFVAAALDFILRDEHAGGR
                     LYLVDPSHPAENCGVLILALGKHGGGELNYSSDTDLIIFFDPASATAQHQSEPVSVFV
                     RMARRLVKILQERTADGYVLRVDLRLRPDPASTAIAISTPAAFFYYESFGQNWERAAM
                     IKARPIAGDLKLGTAFLEQLTPFVWRRYFDYAAIADIHAMKRQIHAVRGHSEIAVAGH
                     DLKLGRGGIREIEFFVKTQQLIYGGRRPNLRGRATLPMLAQLHEQDLVGLEARDELEA
                     AYLFLRELEHRLQMIGDEQTQRLPADPQELASFANFCGYPATAPFAADLVHHFERVAE
                     HYARLFEAAPALSGTAGSLVFTGVSDDPETLETLRGLGFKRPELATETIRGWHFGRRA
                     GVASERAREVLTELIPALLEAFAHTADPDAALTAFDQALAQRIAAVELLSILKSNDSL
                     RNLFADLLGSAPRLADIIIRRPHVLDSMIDQHLLTAKLGETEFMQRLSRVLAAASAAE
                     DVLDGLRDFRQEESFLIGLRLLTADLTPREAGEAHSALAAAIVRAMFEHTTSDFEARF
                     GKVSGGRCVILGMGKLGSHEMTAASDLDMIVIYDFDAAHPDSDGATPSHATNYYTRLS
                     QRLISGLTVATRRGRLYDVDMRLRPSGGQGPLAAQFESFLEYQKTEAETWEHMALTRA
                     CVIAGDASLATEVEAALRKILTMPPPPNLRKDVRDMRRLIAKEKG"
BASE COUNT          597 a          996 c         1060 g          681 t
ORIGIN      
        1 gcccctgata agaagacgcc cctgacacgg ttgcgcagcc gggccgagca ggcgctgcaa
       61 tacggcaaat cgcccgaaga ctaacgggtg gcgctcgaaa aggacatcga ggaatcggat
      121 tcgttgatcc gtattttcaa cgcgttattg atgatagccc gtgccgaggc cggggcgggc
      181 cccgagagcc ttgtcgatgt cgatgccgcg gatgtcgcgc gcgacgtcgg cgagctttat
      241 gaacccgcgg ccgaagagcg tggcttgacc ctgaccgtca cggccgagcc ggggctgatg
      301 ctttacggga gccgcgaact tgtcgggcag gcggtcgcca atctcgtcga taatgcgctg
      361 aaatacggca cgccggacga tggcgcagaa ggcggtgtca gcatttcggc gaagcgccaa
      421 ggccagatga ttgaaatcag cgttggcgac cgcggcccag gaattgcgcc gatcgaccgt
      481 gagcgtgtgc aggaacggtt tgtgcgtctt gagaattccc ggtcgcggcc gggttccggt
      541 cttgggctgt cgctgacggc agctgtcgcg cggctgcata aggggacact gcggtttgag
      601 gataatgcgc cggggctcaa ggccgtgctg tcattgccgg cggcgttggc catgccgccg
      661 aaactccccg cggtcgcgcc gcgcggggaa gcggcctgac gccgggaagg cggacgggca
      721 tgcccgctga acttttgcca ctctacgagc agctggtgaa agcccccgcg ttgagcgatc
      781 ccgttgttgc ggaagcacgg ctggcggcgc tttgttcgag cagcgccgcc gtcacgactt
      841 tggcggacgt gcccgccgtt gcagcgctcc tgaaaggtat tgccgatcat tcgccttatc
      901 tctggaagct cgcgatcgcc gagccggcgc ggctcgcgcg gcttctgacg gccgcaccgg
      961 agatgagcct tgcggattgt ctgatcgatc tccgtgaatc tttgacgggc gctaacgaag
     1021 cgggtgcgat gcgccttttg cgacgcgcca agcaggatgt ggcgcttctc gtcgcgctcg
     1081 ccgatctcgg cggtctttgg acaacccggg acgtgatgag cgcactgacg cgggccgccg
     1141 atcttttcgt tgcggctgcc ctcgatttta tcttgcgcga tgagcacgcg ggcgggcgac
     1201 tttatctagt cgatccatca caccctgcgg aaaattgcgg cgtcctcatt cttgcgctcg
     1261 gcaagcacgg tggcggcgaa ttgaattatt caagcgacac cgatcttatc atcttcttcg
     1321 atccggccag cgcgacggca caacatcaga gtgagcccgt atccgtcttc gtgcggatgg
     1381 cgcgccggct cgtcaaaatt ttgcaggagc ggactgcgga cggctatgtg ctgcgtgtcg
     1441 atcttcggct gcgcccggat ccggcttcga ccgcgatcgc aatttcgacg cccgcggctt
     1501 tcttctatta tgaatccttt ggccagaatt gggaacgtgc ggcgatgatc aaggcgcgcc
     1561 ccatcgctgg cgatcttaaa cttggcacgg cgtttctcga acagctgacg cccttcgtct
     1621 ggcggcggta tttcgattat gccgcaatcg ccgacattca tgcgatgaaa cggcaaatcc
     1681 atgccgtccg cggccattcc gagattgccg tggcggggca cgatcttaaa ttggggcgcg
     1741 gcggcattcg cgagatcgag ttcttcgtca agacccagca attgatctat gggggacggc
     1801 ggccgaatct gcgcggccgg gccacgctgc cgatgctcgc ccaattgcat gagcaagacc
     1861 tcgtcggcct tgaggcgcgc gacgagctcg aagcggccta tctttttttg cgggagctgg
     1921 aacaccgcct gcagatgatc ggcgacgagc aaacgcaacg tctgccggcc gacccgcaag
     1981 agttagcaag cttcgcgaat ttctgcggct atccggcgac agcgcctttt gccgccgatc
     2041 tagtgcatca tttcgaacgc gtcgcggaac attatgcccg actgttcgaa gccgcgccgg
     2101 cactttccgg cacagcgggc agcttggtct tcacgggtgt cagtgacgat ccggagacct
     2161 tggagacatt acgcgggctt ggtttcaaac gcccggaact ggcgacggaa acgatccgcg
     2221 gctggcattt cggccgtcgg gcgggcgtcg cttcggagcg cgcacgcgag gttttgacgg
     2281 agctcatccc ggcattgctg gaggccttcg ctcataccgc cgatcccgat gcggcgctca
     2341 ccgctttcga tcaggctttg gcgcaacgga tcgctgctgt cgaacttctc tcaattctca
     2401 aatcgaatga cagtctgcgc aatttatttg ccgatctcct cggcagtgcg ccgcggcttg
     2461 ccgacatcat catccggcgg ccgcatgtgc tcgattcgat gatcgaccag cacctgctga
     2521 ccgcgaaact gggtgagacc gaatttatgc agcgtctctc tcgcgtatta gcggcggcct
     2581 ctgcggccga agatgtgctc gatggcttac gggatttccg gcaggaggaa tcttttctta
     2641 tcggcctgcg gcttttgacg gcggatctga cgccgcgcga agcgggcgag gcccattcgg
     2701 cgctggccgc cgcaatcgtg cgcgcgatgt tcgagcatac gaccagcgat ttcgaagcgc
     2761 gtttcggcaa agtctcaggc ggacgctgcg tcattctggg catgggaaag ctcggctcgc
     2821 acgaaatgac tgcggcgtcc gatctcgaca tgattgtgat ctacgatttc gacgcggcgc
     2881 accccgactc cgacggcgcc acgccgtcac atgcgaccaa ctattacacg cggctgtcgc
     2941 aacgcctgat ctccggtctt acggtcgcca cgcggcgcgg caggctttac gatgttgata
     3001 tgcgactgcg gccgtcggga gggcaagggc cgctcgcggc acagttcgag agcttccttg
     3061 aatatcaaaa aacggaagcc gagacgtggg agcatatggc cttgacccgt gcctgcgtca
     3121 tcgccggcga tgcatcgctc gcgacggagg tcgaagccgc gctgcggaag atattgacga
     3181 tgccgccgcc cccgaatttg cgcaaagatg ttcgcgatat gcgtcgcctg atcgccaagg
     3241 agaagggcta aaaagatccg ctcgatctga aatatgcgcc tggtggccag gtcgatctcg
     3301 attttctcgc gcaatatctt tgtctgaaac atgc
//
LOCUS       DASWOG010000136         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_223276,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000136 DASWOG010000000
VERSION     DASWOG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223276"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGB93_02740"
     CDS             <1..286
                     /locus_tag="VGB93_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0145078.1"
                     /translation="GAYAFVHFTLLAHPESVTIPSNTLLCRSEGLRVGVVRNGKVQLV
                     PIKIGRDYGDRVEVLSGLTDKDQVIINPSDSLISGIAVHVVADRKSSGAS"
     gene            286..1785
                     /locus_tag="VGB93_02745"
     CDS             286..1785
                     /locus_tag="VGB93_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0145079.1"
                     /translation="MRHAWGGVAAFGFMLGGCMVGPDYVTPAVPITPKFKEATKPVDY
                     KTAGVWVRATPGDAFEHGKWWEVFGDAELDRLEEQLGDANQNLKEAEARFAQSRALIR
                     FARASEFPSVSAGLSAASIRDSANQPYFLTKNPAALGDFEMPIDLNYEVDFWGKVRRT
                     TEAAREESQATAADLSTASLSLHSELALDYIDLRAQDAQQRLLDETVKSFAHALQLTE
                     NRHVGGLAPESDVEQAETQLATTKVQDTDVGVARAQFEHAIAVLIGEPPAALHIPPAA
                     FGQLRPHVVPPMLPSELLQRRPDIAAAERRVGEANQRIGIADAAFYPDVNLAALAGFQ
                     GTTPANWFGWPSLLWSVGTTLAQPVFEGGAIRAQSDSARAAYSENVADYRQTTLSAFQ
                     DVEDNLSSLRILGREARQQRDAVVAANRSLNTFTTLYIGGEVAYLQVITAQQTALSNE
                     VNEVDIQRRRLEANVRLFKALGGGWDKGQLPVLASSGLPRGAFVPFGQQ"
     gene            1795..>2187
                     /locus_tag="VGB93_02750"
     CDS             1795..>2187
                     /locus_tag="VGB93_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0145080.1"
                     /translation="MRSAVEIIEALRLTPHPEGGYFRETFRDTRCFGPRCASTAIYYL
                     LAAGQRSHWHRVDAAEIWHWYAGAPLLLSIKTAEKPATYILGSDLFAGEEPQAIVPAH
                     AWQSAEAQGEWTLVGCTVAPGFEFSQFEV"
BASE COUNT          398 a          668 c          695 g          426 t
ORIGIN      
        1 cggagcctac gcgttcgtgc atttcactct gctcgcccat ccggaaagcg tgacgatacc
       61 atccaacaca ttgctgtgcc gcagcgaagg cttgcgcgtc ggcgttgtgc gtaacggcaa
      121 ggtgcaattg gtgccgatca aaatcggccg tgactacggc gaccgggtcg aggtgctttc
      181 gggcctcacg gataaagacc aggttatcat caatccatcg gactcgctga tcagtggcat
      241 tgccgtgcat gtcgtagctg atcgcaagtc gtccggagcg tcttaatgcg tcacgcctgg
      301 gggggagttg cggcgttcgg gttcatgctg ggcggatgta tggtggggcc ggattacgtg
      361 acgccggctg ttccgattac gcccaagttc aaagaggcga caaagcctgt cgattacaag
      421 acggcgggcg tctgggtgcg cgccacgccg ggcgacgcgt ttgaacacgg taaatggtgg
      481 gaagtcttcg gcgatgcgga actcgaccgg ctcgaagagc aattgggcga tgccaaccag
      541 aacctgaagg aagccgaagc ccgcttcgcc caatcgcggg ctctgatccg gtttgcgcgt
      601 gcgtccgaat tcccgagcgt cagcgccggg ctcagcgcgg cgtcgatccg cgattcagcc
      661 aatcagccgt atttcctgac caaaaatccg gcggcactcg gcgatttcga aatgccaatc
      721 gatctcaatt atgaggttga tttttggggc aaggtgcggc ggacaaccga ggcggcgcgc
      781 gaggaatcgc aggcgacggc cgccgacctc tcaaccgcga gcctcagcct tcattccgaa
      841 ctcgcactcg attacatcga tctgcgcgct caagatgccc aacaacgtct cctcgacgag
      901 acggtaaagt cctttgcgca cgcgctgcaa ctgacggaga accgccacgt cggcggcctt
      961 gcgccggaat ccgatgttga gcaggcggaa acgcagctcg ccacgaccaa ggtgcaggat
     1021 accgacgtcg gcgtggcgcg ggcacaattc gaacatgcga tcgcggtgct gatcggtgaa
     1081 ccgccagccg cgttgcacat tccgccagcg gcgttcggtc agctcaggcc gcatgtcgtg
     1141 ccgccgatgc tgccttcgga gctgttgcag cgccgtccgg atattgctgc ggctgagcgg
     1201 cgcgtgggcg aagccaatca gcggatcggc atagccgacg ccgcctttta tccggacgtg
     1261 aaccttgccg cgctcgcggg ctttcagggc accacgccgg cgaattggtt cggctggccg
     1321 agcttgctct ggtcggtcgg cacgacactg gcgcagccgg tcttcgaggg cggcgccatt
     1381 cgtgcgcagt ctgattccgc ccgcgccgcc tattccgaga atgttgcaga ctatcggcag
     1441 accacgctgt cggccttcca ggacgtcgag gataatcttt cgtcgctgcg cattttgggc
     1501 cgcgaggcga gacagcaacg cgacgccgtc gttgctgcga acaggtcgct caacacgttc
     1561 accacgctct atatcggcgg tgaagtggct tatcttcagg tcatcacggc acagcagacc
     1621 gcgctctcca acgaggtcaa cgaggtcgat attcagcgtc gccgtctcga ggcgaacgtc
     1681 cggctgttca aagcccttgg cggcggctgg gacaaaggtc agcttcccgt gctggcttcg
     1741 agcggcttgc cacgcggcgc cttcgttccg ttcggtcagc aataggcgct gccgatgcga
     1801 tcggcagtcg agatcataga agccctgcgc ctcacgccgc atcccgaggg cggctatttc
     1861 cgcgagacgt ttcgcgacac gcgatgcttc ggcccgcgtt gcgcttcgac cgcgatctat
     1921 tatttgctgg cggccgggca gcgttcgcat tggcatcgcg tcgatgccgc cgaaatctgg
     1981 cattggtatg ctggcgcgcc tctgctttta tcgatcaaaa cggcggaaaa gcccgccact
     2041 tatattctcg gatccgacct ctttgctggc gaagagccgc aggccatcgt gccggcgcat
     2101 gcctggcaga gcgccgaagc gcagggggaa tggacgctcg tcggctgcac agttgcgccg
     2161 gggttcgaat tcagccaatt cgaggtg
//
LOCUS       DASWOG010000137         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_76451,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000137 DASWOG010000000
VERSION     DASWOG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76451"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1973)
                     /locus_tag="VGB93_02755"
                     /pseudo
     CDS             complement(1..1973)
                     /locus_tag="VGB93_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176757.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
     gene            complement(2114..>2492)
                     /gene="rpoZ"
                     /locus_tag="VGB93_02760"
     CDS             complement(2114..>2492)
                     /gene="rpoZ"
                     /locus_tag="VGB93_02760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0145081.1"
                     /translation="EDCIDKVENRFDLVLIAAHRARMIASGTSITVDRDNDKNAVVAL
                     REIAEATLTPEDLKEDFIHSLQKHVEVDEPEAEAVPALTPAVDKVQSDAPGDVQFDRM
                     TEEDLLRGLEGLVPPAETEDDGD"
BASE COUNT          478 a          781 c          741 g          492 t
ORIGIN      
        1 ttgcgcttgc cggcgtccag ttcccaacgc acgtccagcc agcgttccgg ctgatcgtcg
       61 aatgccgtca ggtccggcga ctgaatcgga tagatcgtga cgtgctcgcc cggcgtcagg
      121 atcgcgacga tgcgatcgcc cggcacagcg ccgccgttgg gcgcaaaacg caccggcaga
      181 tcgccgcgca ggccgtaaat cggcatcggc ccggttttgt tttcgtcgcg ctccgggaat
      241 ttgaaaacga gactcgaggc acgcggcaaa ccgaaccagc ccgcttcatt cggcggtggc
      301 attggcggct gcggccccag tttccgttct tcagaaaaat ccggataaac cgctttcacc
      361 acgtcgccgg agaagatttc gccgcggccg acggctgcca gcacgtcctc aacgccggcg
      421 cgcgcaagac gcggcaacgc ggctttcagc ttatcctccg cgtaggtttt gcccgcgcgc
      481 tcgaacgcac gcacgacgat ctgtcgcccg aggcccgtat attgcgcccg caccgcgtcg
      541 cgggcggcgc ggcgaatcgc ggcgcgggcc ttgcccgtca tcaccaggct ttcccagacc
      601 ggcggcggca cctgaccttc ggcgcggatg atctcgacct cgtcgccgtt ttcgagaggc
      661 gcagcaagcg gcgcggcgcg gccgttgatc ttggcgccga cgcacgaatt gccgatcttg
      721 gtgtggaccg cataggcgaa gtcgatcggc gtggcgccgc gcggcaacgc gatcaactgc
      781 cctttcggcg tgaagcaaaa gacctgatcg tgaaagagct cgagcttggt gtgctcaagg
      841 aattcttccg gcgagtcgcc ttccgccagc atgtcgatcg tgcgttgcag ccattgatag
      901 gcgcgacttt catgcgccag cgcctcgcgg ccatcaaccc tgccgtcctt atagaacgcg
      961 tgcgcggcga taccgtagcg cgcgatgttg tgcatgtcat gtgtgcgaac ttgcaactcg
     1021 acacgttgac gccccggccc caccacggtc gtgtggatcg aacggtaatc gttctccttc
     1081 ggtgtcgaaa tgtaatcctt gaaacgaccc ggaacggccg gccatttcga atggatgacg
     1141 ccgataaccc gatagcaatc ctcgattgag ctcacataga cgcggaatcc gaaaatgtcg
     1201 gataattgct cgaaggcgac ggatttgcgc tccatcttgc gccagacgga atagggctgt
     1261 ttctgacgcc cctcgacggt tgcctcgatg ccccgggcgg acaaaagatc gcgcaattcc
     1321 tgctcgacct tggcgatggt cttaccgttc ttctttttaa gatcgtgcag ccgccgtctg
     1381 atcgattagc tcgcctcagg catcaagtgc cggaaggcca gatcctcaag ctcgtcgcgc
     1441 atgctctgca tacccatacg gccggcgagc ggcgcataga tatccagcgt ctcctgagca
     1501 atccgcgcgc gcttgtccgg cggcacgaat tgcagcgtgc gcatattgtg cagccggtcg
     1561 gcgagcttga cgagcaagac gcggacatcg tcggccacgg ccagcagcag tttgcggaaa
     1621 ttctccgcct gcacggcacg tttggagaca agatcgagcc gtttgagctt ggtgaggcct
     1681 tcgacgagac gaccgatctc gggaccgaat agtttgtcga tctcctcgcg ggtcgagtcg
     1741 gtgtcctcga tcgtatcgtg caagacggcg gcgacgattg tcgcgtcgtc gagcttgaga
     1801 tcggtgagga tcgcggcaac ttcgagcgga tgagaaaaat acggatcgcc ggaggcgcgc
     1861 ttttgaatcc cgtgcgcctt catcgcataa acataggcgc gatccagcaa atcctcgtcg
     1921 gcctgcgggt tatagcgccg cacgcgatcg acgagttcat attgccgcat catggttcgg
     1981 gccgatccca tgaagtttag cgcggcgtca aacgcagcgc ctctctccta cgcaaagccg
     2041 gctgccgatc agcccggcaa actttctaaa ctcaacgaag atgggcgggg gcgaaccaac
     2101 agggcgcagc gcctcaatcg ccgtcgtctt cggtctcggc cggaggaacc aggccttcca
     2161 gaccgcgcaa aaggtcttct tcggtcatgc gatcgaattg aacatcgccc ggcgcatcgg
     2221 actgaacctt gtccacggca ggcgtgagcg caggtacagc ctcggcttcc ggttcgtcaa
     2281 cttcgacgtg tttctgcagc gaatgaataa agtcctcttt aagatcctcg ggcgtcagcg
     2341 tcgcctcggc aatctcgcgc aacgccacaa ccgcgttctt gtcgttgtcg cgatcaacgg
     2401 tgatcgacgt gccggacgcg atcatccgcg cgcgatgcgc agcaatcaga acgagatcaa
     2461 atcgattttc gactttatcg atgcaatcct ca
//
LOCUS       DASWOG010000138         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58161,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000138 DASWOG010000000
VERSION     DASWOG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58161"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1855
                     /gene="dnaA"
                     /locus_tag="VGB93_02765"
                     /pseudo
     CDS             338..1855
                     /gene="dnaA"
                     /locus_tag="VGB93_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
     gene            1920..2438
                     /gene="ruvC"
                     /locus_tag="VGB93_02770"
     CDS             1920..2438
                     /gene="ruvC"
                     /locus_tag="VGB93_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0145082.1"
                     /translation="MGAPAIRILGIDPGLRNMGWGIIDVAGSRLTYVASGTIHSIASD
                     DLCRRLRKLHEELAHVINTHAPHEAAVEETFVNRDPQSALKLGQARGIALVVPALAGL
                     PVAEYAANLVKKTVTGTGHAEKAQIALMVKMLLPKSAGAGARGLGADAADALAIAITH
                     AHHYKPLTVRRA"
     gene            2435..>2772
                     /gene="ruvA"
                     /locus_tag="VGB93_02775"
     CDS             2435..>2772
                     /gene="ruvA"
                     /locus_tag="VGB93_02775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0145083.1"
                     /translation="MIGKLRGTIDSVGEDFLILDVHGVGYVVNCSVRTLRSSPEAGAA
                     FALAIETQVREDSIRLYGFSSEVERDWFRLLQTVQGVGAKVALALLGILSADELGAAI
                     ARQDKTTMART"
BASE COUNT          536 a          864 c          826 g          546 t
ORIGIN      
        1 tgggtcgcgc ctaaacacaa ttcatcgcgt cgcgtgcggg catcttgagc ttcgaaatgt
       61 caatttcgaa gatgggggat ttttggctat gataggggct gtcgttgctc ggcaaatttc
      121 tttccgcaga tttccgactt accgatatca cgatgacagt gacttctctc gctatcgagc
      181 gcctttgttg cgttaagagg cggcggcgag agcaataatt gcgcggcctc cgccgcggca
      241 aattctgcaa aacgtcaatc ccatttcacc tttcgcgcag aagacttctg caacggtgct
      301 ttgcgccggc gatattttat tttcgagatg aaggccaatg ttgaacccac gtctcaccga
      361 atccaaccaa gacgcttctg ctccaacagc gaacacgaaa gaatcctggg ctgcgatctg
      421 ccgccggctc aaggccgagc tcggcgaagc gacctacagc tcttggttcc tgcgtcttga
      481 actgacgcag atcggcggcg atgttgccta tctctcggtg ccgacgaaat ttctgaaaat
      541 gtggatccag actcattatt ccgcgcgaat tctcgcagcc gtcggcggcg aatatccaga
      601 ggtcaagcat atcgtcgtca atgtccgctc ttcgacccgc gcgcccgtcg ctgcccacgc
      661 gcaaccgcgt cttgatcagg aagagtttca cgaagcggct gcacctttcg aggctgcgca
      721 cccgcgcggg tacggcgaac cgcgtgccgc cgcgtcgcat tcgaaagata tgaagacatc
      781 ggcccgcacg cagagcgaag cggaaacgct tggcggctcg ccgcttgatc ggcggctgaa
      841 tttctcgacc tttatcgtcg ggcgctcgaa ccagctcgct tatgcatccg cgcaggatgt
      901 cgcctcggcg gcgccgaccg atcccgtgcg gttcaatccg ctctatatcc atgcctcagt
      961 cggtctcggc aaaacgcatt tattgcaggc gaccgcccat gctgcagccg cacagcgccg
     1021 ccgcgtgatc tatctcaccg cctagaaatt catgcacagc tttgccagtg cgcttgaggc
     1081 ctcgacggcg atttccttca aggagaaact gcgcgcgatc gatgttctca tcatcgacga
     1141 tgtgcagttc ctgcgcggca agtcgatcca gcaggaattc tgccacatcc tcaacgcact
     1201 catcgatgca gggcgccaaa tcatcatcgc agcggatcgg ccaccgggcg agcttgaagc
     1261 gcttgaggag cgggtgcgct cacgccttgc gggcggcctc tgcgtcgaaa tgggcgctct
     1321 cgacgagcaa ttgcggctca aaatgcttga agcgcgggtc gccgtggcga agctgctgca
     1381 tccgcagttt gagctttctc cggccgtcct cgcctatgtc gcgagcgtca ttcagacgaa
     1441 cggccgtgat ctcgacggcg ccgtcaaccg gctggttgcg cacgcctcct tgaaccgggc
     1501 acaattgagc ctcgaagcgg cggaacgggc gatccaggac cttatccgcg cgcgcgagcc
     1561 gaaaagggtc aaaatcgagg atatccagaa gctcgtcgcc agccactaca acgtcagcaa
     1621 ggccgacatt ctgtctgccc ggcggaccgc gaacgtcgta cggccgcgcc agatcgccat
     1681 gttcctcgcc aaaacgctga tgccgcgctc cctgcccgag atcgggcggc gctttggcgg
     1741 ccgcgatcac accacggttt tgcatgccgt ccgcaagatc gagggtttgg ttggtaccga
     1801 cgggcggctt tcggaagaga tcgaactgct gaagctgatg ttgaccgaaa attgaccccg
     1861 cagatgggca cgttcgcccc gccgtgggca aacggcgcgg aaagaggtaa agccggagca
     1921 tgggtgcacc ggcgattcgc attctcggca tcgaccccgg cctgcggaac atgggctggg
     1981 gcatcattga tgttgccggc tcgcgcctga cctatgtggc gagtggcacg atccactcca
     2041 tcgccagcga cgatctttgc cgccgccttc ggaagttgca cgaagagctt gcgcacgtca
     2101 tcaatacgca tgcaccgcat gaagctgccg tcgaagaaac cttcgtcaat cgcgacccgc
     2161 aatccgctct gaagctcggc caggctcgcg gcatcgccct cgtcgtcccg gcgctcgctg
     2221 ggctgccggt cgcggaatat gccgccaatc tggtcaagaa gaccgtgacc gggacgggcc
     2281 acgccgagaa ggcgcagatc gccctgatgg tcaaaatgct gctgccgaaa tccgccgggg
     2341 ctggcgcgcg cggcctcggt gccgacgctg ccgacgcatt ggccatcgcc atcacgcacg
     2401 ctcatcatta caagccgctc acggtgcgcc gggcatgatc ggcaagctgc gcggcacgat
     2461 cgattcagtc ggcgaggatt ttttgatcct cgacgtgcat ggcgtcggct atgtcgtgaa
     2521 ctgctcggtg cgaacattac gaagctcgcc ggaagccggg gccgctttcg cgctcgccat
     2581 cgaaacgcaa gtgcgcgagg attccatccg gctctatggc ttttccagcg aagtggagcg
     2641 cgattggttt cggctgctgc agactgtgca gggtgtgggc gcaaaagtcg ccttggcgct
     2701 tctcgggatc ttgagcgccg acgagcttgg ggccgccatc gcgcgacagg acaagacgac
     2761 aatggcgcgg ac
//
LOCUS       DASWOG010000139         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_223296,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000139 DASWOG010000000
VERSION     DASWOG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223296"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGB93_02780"
     CDS             <1..489
                     /locus_tag="VGB93_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEY0145084.1"
                     /translation="ESASGRYPIEAVATMNKIAEEVEHDSFYRQILNAQRPAPEATVA
                     DAIATAARDVAETLDLKAIVAWTSSGSTAFRISRERPEPPVLALTPNRDTARRLALIW
                     GVHAVVTDDAQDVDDMAARACVIAAAEGFASPGERIIIVAGVPFGSPGATNMVRIAYI
                     EK"
     gene            complement(534..1409)
                     /locus_tag="VGB93_02785"
     CDS             complement(534..1409)
                     /locus_tag="VGB93_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145085.1"
                     /translation="MRLRLSPWLVLLAAAAFPLLTIGHAFAGDDDDNAQWPDGSSSEV
                     LKLPGMPPMRLPPGIHIFGSDGRELHVGPEQGLGQPPAYDDGSLAPHHQLPQQKPPED
                     AATRAAKAKAARAEALKQAMAPQPTHEALRTQALDSLFKKLATAHDPDEAGGIAMAIQ
                     HVWMQSDSDTGSLLMERSLAAQQGGNLPLALVLLDKVIALQPDWVEAWNKRAATRLLG
                     GDLNGAIGDFQQVLKLEPRQFSALVALGFIYEKQGFDKRALDSFRKALALDPQQPEIE
                     SIVEKLKTDIEGRDI"
     gene            complement(1460..1585)
                     /gene="ykgO"
                     /locus_tag="VGB93_02790"
     CDS             complement(1460..1585)
                     /gene="ykgO"
                     /locus_tag="VGB93_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY0145086.1"
                     /translation="MKVRNSLKSLRKRHRANQLVRRKGRVYIINKVQKRYKARQG"
     gene            complement(1774..2121)
                     /gene="grxD"
                     /locus_tag="VGB93_02795"
     CDS             complement(1774..2121)
                     /gene="grxD"
                     /locus_tag="VGB93_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0145087.1"
                     /translation="MSNINEQLQSIVDNNDVVLFMKGVPDAPQCGFSNQAVQIFRYID
                     VNFKAVNCLETDELRQGIKSFSNWPTIPQIYLKGEFIGGADILREMFQNGDLFTALSE
                     KGVAFKAPVAAQA"
     gene            complement(2176..2409)
                     /locus_tag="VGB93_02800"
     CDS             complement(2176..2409)
                     /locus_tag="VGB93_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEY0145088.1"
                     /translation="MPMAAAEIEKLIVASIPDAKVEIQDLAGDGDHYAASVVSQEFAG
                     KSKLQQHQLVYKALGGHMGVTLHALQLQTSAPK"
     gene            complement(2409..>3290)
                     /locus_tag="VGB93_02805"
     CDS             complement(2409..>3290)
                     /locus_tag="VGB93_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY0145089.1"
                     /translation="KGLALTTDVNENYCGADPFEGAKQAVAEAWRNLSVVGALPLACT
                     DNLNFGNPEKPEAMGQFVGAVEGLAEACKALDFPIVSGNVSLYNESPEGWILPTPAIG
                     GVGVLDDVTKTVSVSFKNAGETILLIGETQGWLGQSAYLREICGREEGAPPPVDLAAE
                     KKAGDFVRGLVRSGTLTAAHDLSEGGLGIALAEMALAGRLGASIDLATEIPLHAFLFG
                     EDQARYVLTCEPQAAADIVASAKALGIPCQPIGTTGGDALTIPGGAAILLSDLRRQHD
                     SWLPDYMQGQLILEEHA"
BASE COUNT          629 a          997 c         1031 g          633 t
ORIGIN      
        1 gaaagcgcct cgggccgcta tccgatcgaa gccgtcgcga caatgaacaa gatcgccgaa
       61 gaggtggagc acgattcttt ctatcgccag atcctcaacg cgcagcgccc agcaccagag
      121 gcgaccgttg ccgacgccat cgccacggcc gcgcgtgacg tggctgaaac gcttgatctg
      181 aaggcgatcg tcgcctggac atcgtccggc tcgacggcgt tccgtatttc ccgcgagcgg
      241 ccagagccgc cggtgctggc gttgacgcca aaccgcgaca ccgcgcgccg gcttgcgctg
      301 atctggggcg tccacgccgt cgtcaccgat gacgcgcagg acgtggatga tatggcggcg
      361 cgcgcgtgtg tgatcgccgc ggccgagggc ttcgcgtccc ccggcgagcg gatcatcatt
      421 gtcgccggcg tgcccttcgg ctcccccggc gcgaccaata tggtgcggat cgcctacatc
      481 gagaagtaga gtggccgcga gcggagcgac attcgcttcg ctcgcaatga cgactaaata
      541 tcgcggcctt cgatgtcggt cttgagcttt tcgacgatcg attcgatctc gggctgctgc
      601 ggatcaagtg ctagcgcctt gcggaaagaa tcgagagcgc gcttgtcgaa accctgcttc
      661 tcgtaaatga agccaagcgc gaccagcgcc gaaaactgcc gcggctcaag ctttaaaacc
      721 tgctggaagt caccgattgc gccattgaga tcaccgccga gaagtcgcgt tgcggcgcgc
      781 ttgttccaag cctcaaccca atctggttgg agagcgatca ctttgtcgag caagacgagc
      841 gccaaaggca gattgccgcc ctgctgtgcc gcaagcgagc gctccatcaa aaggctcccg
      901 gtgtcggaat ccgactgcat ccagacatgt tgaatcgcca tcgcgattcc acccgcctca
      961 tcgggatcgt gggccgtcgc gagctttttg aagagcgaat cgagggcctg cgtacgcagc
     1021 gcttcatgcg tcggctgcgg cgccatcgcc tgcttcaagg cctcggcacg cgcggccttg
     1081 gctttggcgg cacgggttgc tgcatcttcg ggtggctttt gctgcggcaa ttggtgatgc
     1141 ggggcgagcg agccgtcgtc gtaggctggc ggctgtccaa gtccttgctc gggaccgaca
     1201 tgtagctcgc ggccatcaga cccgaaaata tggatgcccg gcggcagccg catcggcggc
     1261 atcccgggca gtttcaggac ttcgctggaa gacccgtcag gccattgcgc attatcgtca
     1321 tcatcgccgg cgaaggcgtg accgattgtc agcagcggaa aggcagcggc ggcgagcaga
     1381 accagccatg gcgaaaggcg cagacgcatc tgctcaaatt agaggagagc cggagaatgt
     1441 caaagaacgc gcgagaagat cagccttgcc gagctttgta gcgcttctgc accttgttga
     1501 tgatgtagac gcggcccttg cggcgcacca attggttggc gcgatgacgc ttgcgcaggg
     1561 acttcaaaga attgcggact ttcatgacct cagcctagca aatcgagccg gcggcagagc
     1621 cggcaaaatc gggatttcgg gagcgcctgt tcagacctga gggcgtcgcg cccggccggc
     1681 tcaagctaaa acgcggccag aggccgcgcg tcgtggcggt gctatgcccg atttcgcggg
     1741 ctcaatcaag cccgcagggt cacactttgg cgcttaggcc tgggcggcga ctggggcttt
     1801 gaacgcaacg cccttttcgc tgagcgccgt gaacaagtcg ccgttctgga acatctcgcg
     1861 caggatgtcg gcgccgccga tgaattcgcc cttgagataa atttgcggaa tcgtcggcca
     1921 gttcgagaag ctcttgattc cctgacgcaa ttcgtcggtc tcaaggcaat tcacggcttt
     1981 gaagttgacg tcgatatagc ggaaaatttg cacggcctga ttggagaagc cgcattgcgg
     2041 ggcgtccggc acacccttca tgaacagaac gacatcgtta ttgtcgacaa tcgactgaag
     2101 ctgctcgttg atattggaca tactcggtcc ttctttgatc gtttacgctc tgaggcgcga
     2161 taatgcgtat atgcgttatt tcggggcgga ggtctgcagt tgcaaggcat gcagcgtgac
     2221 gcccatgtgg ccgccgaggg ccttgtagac gagctggtgc tgctgcagct tggatttgcc
     2281 agcgaattcc tgcgagacca cgctcgccgc ataatgatcg ccgtccccag ccaggtcctg
     2341 aatttcgacc ttggcgtcgg gaatcgacgc gacgatcaat ttttcgatct cggccgcggc
     2401 catcggcatc aggcatgctc ctcgagaata agttgaccct gcatataatc cggcagccat
     2461 gaatcgtgct ggcgccgcag gtcactgagc aatatggcgg cgccccccgg aatggtcaac
     2521 gcatcgccgc ccgtcgtgcc gatcggctgg cacgggatgc caagagcctt ggcgctggca
     2581 actatatcgg cggcagcctg cggctcgcag gtcagaacgt agcgcgcctg atcctcgccg
     2641 aagaggaagg catgcagcgg gatctccgtt gcgagatcta ttgaagcacc aagacggccg
     2701 gccagcgcca tctccgccag cgcgatgcca aggccgcctt cggagagatc atgggccgca
     2761 gtgagcgttc cagaacggac gaggccgcgg acgaaatcgc ccgccttctt ttcggcggcg
     2821 agatcgacgg gcggtggcgc gccctcctcg cggccgcaaa tttcacgcag ataggcggat
     2881 tggccgagcc agccctgcgt ctcgccgatc aacagaattg tttcgccggc gtttttgaag
     2941 ctgaccgaaa ccgtcttggt gacatcgtcg aggacgccga ctccgccgat ggccggagtc
     3001 ggtaagatcc acccctcggg gctctcgtta tagagcgaga cattgccgga gacgatcggg
     3061 aaatcgagcg ccttgcaggc ctcggccaag ccttcgaccg cgccgacgaa ttgccccatc
     3121 gcctcgggct tctcgggatt gccgaaattg agattgtcgg tgcaggcgag cggcagtgcg
     3181 ccgacgacgc tgagattgcg ccacgcttcg gcaaccgcct gtttggcgcc ctcgaacggg
     3241 tcagcgccgc aatagttttc attcacgtcg gtcgtcagcg ccaagccttt
//
LOCUS       DASWOG010000140         6842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_39749,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000140 DASWOG010000000
VERSION     DASWOG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39749"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB93_02810"
     CDS             complement(<1..248)
                     /locus_tag="VGB93_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEY0145090.1"
                     /translation="MSLLHFIRRIVLALLACLPLIGAARAREVTDIAGRHVTVPDKVT
                     RVVLGEGRLVYALALLDRADPFQRVVGWQNDFRLMDPHT"
     gene            complement(324..1301)
                     /gene="tal"
                     /locus_tag="VGB93_02815"
     CDS             complement(324..1301)
                     /gene="tal"
                     /locus_tag="VGB93_02815"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY0145091.1"
                     /translation="MPSKLDQLKQWTVVTADSGDIGAIAQYKPQDCTTNPSLILKAAG
                     MPAHQELVEEAVLWGRSHGATTSRITQRLAVNFGAALTKLVPGRVSTEVDADLSFDTE
                     GTINQAREIISDYHQRGVGRERVLIKIAATWEGIQAAAHLQKEGIACNLTLVFCLAQA
                     AAAAEAGIALISPFVGRILDWHVKTSGETYTAETDPGVLSVKQIYAYCKAYGENTIVM
                     GASFRNRGEIEALAGCDSLTIAPALLDQLAADQGTLARQLSPDAKLLAPAQMHLDEKS
                     FRCMLNEDAMATEKLAEGIRTFSRDLQELRELVSARLAQSAASDALGPL"
     gene            complement(1359..2462)
                     /locus_tag="VGB93_02820"
     CDS             complement(1359..2462)
                     /locus_tag="VGB93_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0145092.1"
                     /translation="MKRSRLLLVFVLFARCALTSAVAQNYIIEDIRVPDSAAGSQGLE
                     GVFMRPTEHRRYPLALISHGSPRDPAERPNLTPQQMLPQMMEFARRGWATAAILRRGY
                     GQSGRGWAEGFGPCDHADYLRAGRAGAADLRSAFAYLSKRSDVDPHRMIAIGVSAGGF
                     ATVALTENPPRGLLAAISFAGGRGSENADTVCDAPALIEAFHIFGSRSRVPLLWVYAA
                     NDHFFGPILADQFRQAFAGAGGNVTFIHAEAFGSDGHQLFSSSGRQIWAPLVDSFLAR
                     QHLPRADPLLPPYASPPLAPPRQLSASGRKDFAAYLAAFPHKAFAISDNGAYGWRTAR
                     RVDDDAKSGALGLCGAHAATCHVYAVDDRLSSQ"
     gene            complement(2490..4319)
                     /gene="thiC"
                     /locus_tag="VGB93_02825"
     CDS             complement(2490..4319)
                     /gene="thiC"
                     /locus_tag="VGB93_02825"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0145093.1"
                     /translation="MNIRQKPENLIPQSVTCGPLPGAQKVYAEPRAGIRVPFREITLE
                     PSANEPPVRVYDASGPYTEEAPRVDLSAGLPHTREAWYASRGFESYTGRAIKPEDNGN
                     VGTDKLVPPCPANVAPLRGTPGKPVTQYEFARAGIITEEMIYVATRENLGREKMLANA
                     EEKLADGESFGAAIPAFVTPEFVRSEIARGRAIIPANINHPELEPMIIGRNFLVKINA
                     NIGNSAVTSSVAEEVDKMVWSIRWGADTVMDLSTGRNIHNIRDWIIRNAPVPIGTVPI
                     YQALEKVDGDPIKLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYVPLTAKRTTGIVSR
                     GGSIMARWCLSHHQESFLYTHFDEICDIMRAYDVSFSLGDGLRPGSIADANDRAQFAE
                     LETLGELTKIAWDKGCQVMIEGPGHVPMHKIKVNMEKQLEECGEAPFYTLGPLTTDIA
                     PGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPDRDDVKTGVITYRIAAHAADLAK
                     GHPAAQVRDDALSRARFDFRWQDQFNLSLDPETARLFHDETLPKDAHKVAHFCSMCGP
                     QFCSMKITQDLREEVNAMAEREKGMADKSAEFLEKGGKLYVSQ"
     regulatory      complement(4391..4492)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4930..5643)
                     /locus_tag="VGB93_02830"
     CDS             complement(4930..5643)
                     /locus_tag="VGB93_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0145094.1"
                     /translation="MPQAAPSDAPSTILIIGASRGLGLAMAVEFLKKKWNVVGTVRGK
                     TRTLLHDLADKFEGRVEIEILDISEPDQLAALREKLSGRVLDILFVNAGVTNNKAETP
                     GDVTTDEFVRVMVTNALSPMRVLESLKKYVPATGLIGVMSSGLGSITNNVTGTREVYR
                     SSKAALNMFMRSFAARHSATSRAMVVMAPGWVRTDLGGPDAPLTIEDSVPNLVNVLLS
                     KQGKPGLEYLDYLGRTVPW"
     gene            complement(5806..6375)
                     /gene="efp"
                     /locus_tag="VGB93_02835"
     CDS             complement(5806..6375)
                     /gene="efp"
                     /locus_tag="VGB93_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0145095.1"
                     /translation="MKVIASSIRKGNILEREDGQLYVVLTAESFHPGKGTPTTQIDMR
                     RLSDGVKTTDRYKTTEQVERAFVEDHDYSYLFQDGEHYTFMNGETYDQVNVSADVIGE
                     QAVYLQEGMVCILSLFNGIPVAIQFPPRVTLEIVETEPAMKGQTASSSYKPAKLSNGA
                     RTMVPPHIQPGTRVVIQTEDGSYVERAKD"
     gene            6569..>6842
                     /locus_tag="VGB93_02840"
     CDS             6569..>6842
                     /locus_tag="VGB93_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0145096.1"
                     /translation="MSKPSFWWEPAAHAEKRRFLTARAALAKAARGFFDARGFIEADT
                     AVLQISPGNETHISAFATEMIAPDEARSRLYLHSSPEFALKKLLAAG"
BASE COUNT         1296 a         2074 c         2176 g         1296 t
ORIGIN      
        1 gtatgcgggt ccatcagccg gaaatcgttc tgccagccga cgacgcgctg aaacgggtcg
       61 gcgcgatcga gcagcgccag cgcatagacc agccgcccct cgcccagaac gacgcgcgta
      121 accttgtccg gcacagtgac atgccgcccg gcgatgtcgg tgacttcgcg agcacgcgca
      181 gcgccgatca gcggcagaca ggccagcagg gcaaggacga tccggcggat gaaatggagc
      241 agggacatgg acgtgggagg ttgcggcgca caatggccac cgctgtccga gccgccgtcc
      301 aaaagttccg caacagcaat gatttagagt ggtcctaagg catcgctggc ggccgattgc
      361 gccagccgcg ccgagacaag ttcgcgcaac tcttgcaggt cgcgcgagaa agtgcggatg
      421 ccttcggcga gcttctccgt cgccatcgcg tcctcgttga gcatgcagcg gaagcttttc
      481 tcgtcgaggt gcatctgcgc cggggcgagc agcttggcgt cgggtgaaag ttgccgcgcg
      541 agcgtccctt ggtccgccgc caattgatcg agcagcgccg gggcgatcgt caggctgtcg
      601 cagccggcca aggcctcgat ctcaccccga ttgcggaacg acgcgcccat cacaatcgtg
      661 ttttcgccat aagctttgca ataggcgtag atctgcttga ccgagagcac gccggggtcc
      721 gtctcggcgg tataggtctc gccgctagtc ttcacatgcc aatcgagaat gcggccgacg
      781 aagggcgaga tgagcgcgat gccagcttcg gcagcggccg ccgcctgcgc caggcagaag
      841 accaacgtga gattgcaggc gatgccttcc ttttgcaggt gtgctgcggc ctgaatcccc
      901 tcccaggtcg cggcgatctt gatcagcacc cgctcacggc cgacgccgcg ctggtgataa
      961 tcgctgatga tctcgcgcgc ctgattgatc gtgccttcgg tatcgaagga gagatcggca
     1021 tcgacttcag tcgagacgcg gcccggaacg agtttcgtca gcgccgcgcc gaaattgacg
     1081 gccagccgct gcgtgatccg gctcgtcgtg gcgccgtggc ttcggcccca gagcaccgct
     1141 tcctcgacaa gctcctgatg cgccggcatt cccgccgcct tgaggatgag cgacggattg
     1201 gtcgtgcaat cctgcggctt gtattgcgcg atggcgccga tatcgccgct atcggcggtg
     1261 acgacggtcc attgcttcaa ttgatcgagt ttggagggca tggggtctat ccggtggtcg
     1321 ttgggctaac gcgtcgggcg ccgcaagttt ccgcacgctt actgcgatga aagccgatcg
     1381 tccacggcat aaacatgaca ggtcgcggca tgggcgccgc aaaggccgag cgcgccgctt
     1441 ttggcgtcgt catcgacgcg ccgcgccgtg cgccagccgt aggcgccgtt atcgctgatt
     1501 gcgaaggcct tatgcgggaa ggcggcaaga taggcggcaa aatccttgcg cccgctcgcg
     1561 gaaagctggc gcggcggcgc gagtggcggc gacgcatagg gcggcagtag cggatcggca
     1621 cgcggcagat gctggcgcgc cagaaagcta tcgacgagcg gcgcccagat ctgccgcccc
     1681 gacgacgaga aaagctggtg cccgtctgag ccgaaggcct cggcatggat gaatgtcacg
     1741 ttgccgccag cgccggcgaa cgcctggcgg aattgatcgg ccagtatcgg gccgaagaaa
     1801 tgatcgttgg cggcatagac ccagagcagc ggcacacggg agcgcgagcc gaatatgtgg
     1861 aacgcttcga ttagggccgg cgcatcgcag actgtatcgg cgttctccga gccgcggccg
     1921 ccggcaaagc tgatcgcagc cagaagaccg cgtggcggat tttccgtcag cgcgacggta
     1981 gcgaagccgc cggccgagac gccgattgcg atcatccggt gcggatcaac gtcggagcgt
     2041 tttgagagat aagcaaaggc ggagcgcaga tcggcggcgc cggcgcgtcc ggcgcggaga
     2101 taatcggcgt gatcgcacgg cccgaaacct tccgcccagc cgcggccaga ctggccatag
     2161 ccgcggcgca ggatcgccgc cgttgcccag ccgcgccggg cgaattccat catctgcggc
     2221 agcatctgct gtggcgtcag gtttggcctc tcggcgggat cgcgcggcga accatgactg
     2281 atgagcgcga gcggatagcg ccggtgttca gtcggacgca tgaagacgcc ttcgagccct
     2341 tgcgatccgg cagcagagtc cggaacgcgg atgtcttcga tgatgtaatt ttgcgcgacg
     2401 gcggaggtta gagcgcaacg cgcaaaaagc acgaatacca aaagcaagcg ggagcgtttc
     2461 atatgccgac gttagcgtaa tccgatagat tattgcgata cgtaaagttt cccgcccttt
     2521 tccaagaatt ccgcgctctt gtccgccatg cccttttcgc gttcggccat cgcattcact
     2581 tcctcgcgca aatcctgcgt gatcttcatc gagcagaatt gcggcccgca catcgagcag
     2641 aaatgcgcga ccttgtgcgc gtccttcggc agcgtctcgt cgtggaacag ccgtgccgtt
     2701 tccggatcga gtgacaggtt gaactgatcc tgccagcgga agtcgaagcg cgcgcgtgag
     2761 agcgcatcgt cgcgcacctg cgccgccgga tgacccttgg cgagatcggc ggcatgcgcc
     2821 gcgatccgat aggtgatcac ccccgtcttc acatcgtcgc ggtcgggcag gccgagatgt
     2881 tccttcggcg tgacgtagca aagcatggcg cagccgaacc agccgatcat cgccgcgccg
     2941 atgcccgacg tgatgtgatc gtagcctggc gcgatgtcgg tcgtcagcgg cccgagcgta
     3001 tagaacggcg cctcgccgca ttcctcgagc tgcttttcca tattcacctt gatcttgtgc
     3061 atcggcacat ggccggggcc ttcgatcatc acctgacagc ctttgtccca ggcgatcttg
     3121 gtgagttcgc cgagcgtttc cagttcggcg aattgcgcgc ggtcgttggc gtcggcgatc
     3181 gagcccggac gcagaccatc gccaagcgag aacgagacat cgtaagcacg catgatgtcg
     3241 caaatctcgt cgaagtgggt gtagaggaag ctctcctggt gatgcgacag gcaccagcgc
     3301 gccatgatcg agccgccgcg cgagacgatg cccgtcgtgc gcttggcggt gagcggcaca
     3361 taggcgaggc gcacgccggc gtggatggtg aaataatcca ccccctgctc ggcctgttcg
     3421 atcaaagtgt cacggaagat ttcccacgtc agcttgatcg gatcgccatc gaccttttcg
     3481 agcgcctgat agatcggcac cgtgccaatc ggcaccggcg cgttgcggat gatccagtcg
     3541 cggatgttgt ggatgttgcg gccggtcgaa aggtccatga ccgtatcggc gccccagcgg
     3601 atcgaccaga ccatcttgtc gacttcttcc gcgacagagg acgtgacggc ggaattgccg
     3661 atattggcat tgatcttcac caggaagttg cggccgatga tcatcggctc aagttcgggg
     3721 tgattgatgt tggccggaat gatggcgcga ccgcgcgcga tttcgctgcg gacgaattcc
     3781 ggtgtgacga atgcaggaat tgcggcgccg aaactttcgc catcggcgag cttctcttcg
     3841 gcgttcgcca gcatcttctc acggccgaga ttttcgcgcg tcgcgacata gatcatctct
     3901 tcggtgatga tccccgcgcg ggcgaattcg tattgcgtca ccggcttgcc cggcgtgccg
     3961 cgcaagggcg cgacattggc ggggcagggc ggcacgagct tgtcggtgcc gacattgcca
     4021 ttgtcttccg gcttgatggc gcggccggta taagactcaa atccccgcga cgcataccaa
     4081 gcctcacgcg tgtgcggcag gccggcggag agatcgacgc gtggcgcctc ctcggtgtag
     4141 gggcccgacg catcatagac acgcaccggc ggctcgttgg ccgaaggttc gagcgtaatt
     4201 tcgcggaacg gcacgcgaat gcccgcgcgc ggttccgcat agaccttctg cgcgccggga
     4261 agcggcccgc acgtgacgct ctgcggaatg agattttcgg gcttttggcg aatgttcatc
     4321 gtccggctcc gcgctttgtt cggaatggcg gcgcgggagc agatacgaag gcgcagatgg
     4381 attccacccg tgctttgcgt attgcctgtc cctgcgctgg cattacccag tttcaggttc
     4441 gaagggtctg atctcagccg ccgaacttcc ggcagcaccc caaggctccg ggcaggatag
     4501 gcgcggatgc gccacgctgc aagtgcgtga aatgcgccgg aacggaatgc gccaggccac
     4561 gttggccaaa cgaacggcga cgatgtccgc gacgcgtaga gcaggagcgt tagggaaaac
     4621 caggtgaata gggctgacaa tgatggatat gaataagggc tgggaaccga tgcccccgca
     4681 aaatgctatc ccgacgcgac gccttgagcg ccagcgttcg gaattaccgc gcggtggaaa
     4741 agatatggcg gcccaaatcg gggcagaact gcgcctcgtg ttcgccccgg tcccgatgaa
     4801 caaattagcg ggcagcgacg atctctttgc gcgaatcgac cagtggaacg acgatatcgg
     4861 ctaagcttgc ggcggctaag tccttggcgc gctcgacgca agagccgtct tctgtctgga
     4921 tgacaacccc taccaaggaa cggtacggcc gaggtaatcg agatattcca gcccaggctt
     4981 gccctgcttc gagagaagca cgttgaccaa gttcggcaca ctatcttcga tggtcaaggg
     5041 tgcatccggc ccgccgaggt cggtgcggac ccagccgggc gccatcacca ccatcgcgcg
     5101 cgatgttgcg gagtgacggg cagcgaagct ccgcatgaac atgttcaacg ccgccttgct
     5161 gctgcgatag acttcccgcg tgccggtcac attgttggtg atgcttccaa gcccggacga
     5221 catcacaccg atcagacccg tcgccgggac atattttttc agactctcga ggacacgcat
     5281 gggactcagc gcattcgtga ccatgacgcg gacaaactcg tccgtcgtta catccccggg
     5341 cgtttcggcc ttgttgttcg tcacgcccgc gttgacaaac agaatatcga gcactctgcc
     5401 cgacagcttt tcgcgcaagg ccgcgagctg atccggctca gagatgtcga gtatttcgat
     5461 ctcgacacgg ccctcgaact tgtccgccag gtcgtgcagc aatgttcggg tttttcctcg
     5521 aactgtgccg acgacattcc attttttctt caagaattcg accgccatgg cgaggccaag
     5581 accgcgcgag gctccgatga taaggatcgt gcttggggcg tcgctcggag cggcttgggg
     5641 catgacgtct tccagtagcg ggatggatgc caaatagcgc acgccgcatg accgacgcga
     5701 gccgcgtcgg atgcaaatta tcgtgacccg ccgcctatgt cagaatcgga ctccaatcgc
     5761 ccgccggatg acaattccaa gtggtaagat gattgtgctt cgaccctaat ccttggcgcg
     5821 ctcgacgtaa gagccatctt ccgtctggat gacaacgcgg gtgcccggct gaatatgcgg
     5881 cggcaccatg gtgcgggcgc cgttcgacaa tttcgccggc ttataggacg acgacgccgt
     5941 ctggcccttc atcgccggtt ccgtctcgac gatttcaagc gtcacgcggg gcggaaactg
     6001 aatcgcgacc gggatgccgt tgaagagcga aagaatgcag accatgcctt cctgcaggta
     6061 gaccgcctgc tcgccgatca catcggccga gacgttgacc tgatcatagg tctcgccatt
     6121 catgaacgta taatgctcgc cgtcctgaaa aaggtagctg tagtcgtgat cctcgacgaa
     6181 ggcgcgctcg acctgttccg tcgtcttgta gcgatccgtg gtcttcacgc cgtcggacag
     6241 ccggcgcatg tcgatctgcg tcgtcggcgt gcccttgccg ggatggaagc tttcggcggt
     6301 cagcacgaca taaagctggc cgtcctctcg ctcgagaata ttgcccttgc ggatggaact
     6361 ggcgatgact ttcacgaggt ttccttcgtc tagctcgaat ggcgctttga tccgagccgc
     6421 cgcttggagc aaaacacatt aaaattcaat atggatcggc cgaaactggc taatttcggc
     6481 cggactgccg cgcggcgcac ggctgcgcta taagcgcccg cgccctcgct acccggcttg
     6541 gcgaggggtt ttcaagcgaa atgaggaaat gagcaagcct tctttctggt gggagcctgc
     6601 ggcgcacgcc gaaaagcggc gctttttgac cgcacgggcg gcgctggcca aggccgcgcg
     6661 cgggtttttc gacgcccgcg gcttcatcga ggccgacacc gccgtcctcc aaatatcgcc
     6721 cggcaacgag acgcatattt cggccttcgc gactgaaatg atcgcgccag acgaagctag
     6781 atcgcggctt tatctgcatt cgtcgccgga attcgcgctg aaaaagctgc tggcggcggg
     6841 cg
//
LOCUS       DASWOG010000141         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_113438,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000141 DASWOG010000000
VERSION     DASWOG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113438"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGB93_02845"
     CDS             complement(<1..518)
                     /locus_tag="VGB93_02845"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY0145097.1"
                     /translation="MHDPFDASTIIFALLAIFVLWKLRSVLGTRGGAEKPPAQNTSSF
                     FKTSTAANENKVVPLPGSGPRPAPPPPPLADPERWKAYAEPGSKVANGLDAIAAADPN
                     FGLDQFIAGAKSAYEMIVTAFATGDRELLARLLDKDVFESFNSAIDARAARGESLMTK
                     VVSIDKTGVFDAG"
     gene            639..1064
                     /locus_tag="VGB93_02850"
     CDS             639..1064
                     /locus_tag="VGB93_02850"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEY0145098.1"
                     /translation="MPYIAAFFLGTCLWILAELSVFGMIASAIGLPGAIALTLIFSLL
                     GLALLRRLGGQARQLFQTVAENPAHALRLSPLTLRSGALAALGAILLVIPGFLSDCVG
                     LALLLPSWRHQELAAKPRDPNVIDLTPQDWHRIDDKERG"
     gene            1158..1634
                     /gene="secB"
                     /locus_tag="VGB93_02855"
     CDS             1158..1634
                     /gene="secB"
                     /locus_tag="VGB93_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY0145099.1"
                     /translation="MADGNGSGAAATAPTLNAMVQYTKDFSFENPNAPRSLGPQPKAP
                     NISIQVNVNARQIAEADYEVELLLEGAAGEGNDTLFKFELNYAGIFRVLNIPESDMQP
                     VVMIECPRLLFPFARQIVADAVRNGGFPPLYIDPIDFAGLYRQRMAQTAQTPIAQA"
     gene            complement(1710..2408)
                     /gene="dnaQ"
                     /locus_tag="VGB93_02860"
     CDS             complement(1710..2408)
                     /gene="dnaQ"
                     /locus_tag="VGB93_02860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY0145100.1"
                     /translation="MREIVFDTETTGLDPASGHRIVEIGCIEILNSIPTGQIFHFYLD
                     PERDMPEEAFRVHGISAESLIGKPKFAEIAEKFIAFVGDATLVAHNAEFDLRFVNAEL
                     TMLGHPPLGLERIVDTLALARRRHPGSPASLDALCQRYGIDASRRTRHGALLDAELLA
                     EIYAELTGGRQTSLLLQTAAVNAQRGAAARPVRPSPLAPLIAATEWAAHAAFVATLGD
                     KAIWRRYLPKSGEV"
     gene            complement(2401..3006)
                     /gene="coaE"
                     /locus_tag="VGB93_02865"
     CDS             complement(2401..3006)
                     /gene="coaE"
                     /locus_tag="VGB93_02865"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912480.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0145101.1"
                     /translation="MFVLGLTGSIGMGKSVTAAIFREAGIPVHDSDAAVHALYRGAAV
                     PLVEAAFPGTLRDGQIDRTRLSAYVVNDPAALARLEAIVHPLVRAGRAEFAAEARASG
                     ADLIVLDIPLLFEIGCESEVDAVLLVTAPEDVQKARVAGRPGMTEAKLAAIIAKQMPD
                     TDKRRRADMILDTSLGHDMAARQVHRLIDDIRAGRVARHHA"
     gene            complement(3006..>3640)
                     /locus_tag="VGB93_02870"
     CDS             complement(3006..>3640)
                     /locus_tag="VGB93_02870"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0145102.1"
                     /translation="FAACAVTTPVARQLQAVNTLWIEDEKLFGDNTDVAGFLASLDAD
                     VPDWDKSGDKAVVLGAGGAARGIVFALNARGIRDIAIVNRTRSRGEQLQRQFPDARID
                     LADFAVLPALLRDADLLVNTKSLGMIGQPPLDIDLAPLPARAVVADIVYAPLETALLR
                     QASARGLRTSGGLGMLLHQAVPGFARWFGQTPQVTPDLRARVAADIADKS"
BASE COUNT          690 a         1123 c         1142 g          685 t
ORIGIN      
        1 ccagcatcga aaacgccggt cttatcgatc gaaacgactt tggtcatgag cgattcgccg
       61 cgcgccgcgc gggcgtcgat ggcgctgttg aaactctcaa agacatcttt gtcgaggagc
      121 cgcgccagaa gctcgcggtc gccggttgca aaggccgtca cgatcatctc gtaagcgctt
      181 tttgcgccgg cgatgaattg atcgaggcca aaattcggat cggcggcggc gattgcgtcg
      241 aggccgttgg caactttcga gcccggctcg gcataggcct tccaacgctc gggatccgcc
      301 aaaggcggcg gaggcggggc cgggcgcggc ccgctgccgg gcaacggcac aaccttattt
      361 tcattggcgg cagtgctggt cttgaagaac gaactcgtgt tctgcgccgg cggcttctcc
      421 gcgccgccgc gcgtgcccag aacggagcgc agcttccaca atacgaagat ggcaagaagc
      481 gcgaaaatga tcgtcgaggc gtcgaagggg tcgtgcattt aacttaggct cggggcttgg
      541 gagcggattc accgttcgga cgacatatat agcgtccaac cgggaaagtt cataagctga
      601 tagtctgccg gcagactttg tgttttctag gacgttctat gccttatatc gccgctttct
      661 ttttgggcac ctgcctctgg atcctcgccg aactgtcggt gttcggcatg atcgccagcg
      721 caatcggcct gccgggcgcg attgcgctca ctttgatttt cagcctgctc gggctcgcgt
      781 tgctgcgccg gcttggcggc caggcgcgtc agctttttca aactgtcgcc gaaaatccgg
      841 cccacgcctt gcggctttcg ccactgacgc tgcgcagcgg cgcgctggcc gcgcttggcg
      901 caatcctgct cgtgatcccg ggctttctgt cggattgcgt cggcctcgca cttttgttgc
      961 cgtcgtggcg tcatcaggaa ttggcggcca agccgcgcga tccgaatgtc atcgatctga
     1021 ctccgcagga ctggcatcgc atcgacgaca aggagcgcgg ctgaggctcg cttgttcggc
     1081 gcccccatcc atgctaggcg ctggcgcgat tttcagggtg gctaagccgc ttcggtttcg
     1141 gtggggagag taacgacatg gcggacggca acggcagcgg tgcggcagca acagcgccca
     1201 ctcttaatgc catggtgcaa tatacgaagg atttttcctt cgagaatccg aatgcgccac
     1261 gctcgctcgg accgcagcca aaggcaccga atatctccat tcaggtgaac gtcaacgccc
     1321 gccagatcgc cgaggccgat tacgaagtcg agctcttgct cgaaggcgcg gccggcgaag
     1381 gcaacgacac gctgttcaaa tttgaactga attatgccgg tattttccgt gtgctcaaca
     1441 ttcccgagag cgacatgcag ccggtcgtca tgatcgaatg cccgcggctg ctgttcccat
     1501 tcgcccggca aatcgttgcc gatgcggtgc gcaacggcgg cttcccgccg ctttacatcg
     1561 acccaatcga tttcgccggc ctttaccgcc agcggatggc gcagaccgca cagaccccga
     1621 tcgcacaagc gtgatcccga aaagttgcag acttttcgga caagatcacg cccaagaaaa
     1681 tctagagcac gatctttccg aatcgcgctc tatacttctc ccgatttcgg caggtagcgg
     1741 cgccagatcg ccttgtcgcc gagcgtcgcg acaaaggcgg cgtgcgcggc ccattccgtc
     1801 gcggcgatga gcggcgcaag cggcgacggg cgaaccggtc gcgcggccgc cccgcgttgc
     1861 gcgttaacgg cggcggtctg gagcagcagg ctcgtctgac ggccgcccgt caattccgca
     1921 tagatttcgg cgagaagttc cgcatcgagc aaagcgccat gcctggtgcg ccggctggca
     1981 tcgatgccgt agcgttggca gagcgcgtcc aagctcgccg gcgaaccggg atgacggcgg
     2041 cgtgccagcg ccagagtatc gacgatccgt tccagcccga gcggcggatg gccaagcatg
     2101 gtgagctcag cattgacgaa gcgcagatcg aactcggcat tatgcgcaac gagcgtggcg
     2161 tcgccgacga aagcgataaa cttctcggcg atctcggcga acttcggttt tccgatcagc
     2221 gattccgccg aaattccatg aacgcggaac gcctcctccg gcatgtcgcg ctcagggtcg
     2281 agatagaagt gaaatatctg tccggtcgga atggaattca gaatttcgat acagccgatc
     2341 tcgacgatgc gatggcctga ggccggatcg agaccggtcg tttccgtgtc gaagacgatc
     2401 tcacgcatga tgtcttgcca cgcgtccggc acggatatcg tcgatgagcc tgtgcacctg
     2461 gcgcgcagcc atgtcgtgac caaggctcgt gtcgaggatc atgtcggcgc ggcggcgttt
     2521 gtcggtgtcg ggcatttgtt tggcgattat ggcggcgagt ttcgcttctg tcatgcccgg
     2581 tcttccagca acgcgcgcct tttgcacgtc ttccggcgcc gtgacgagca gcaccgcgtc
     2641 gacttcactc tcacagccaa tttcaaacag aagcggaatg tcgaggacga tgagatcggc
     2701 acccgaggca cgggcctcgg cagcaaattc ggcgcgcccg gcgcgcacga gtgggtggac
     2761 gatcgcttca agccgcgcca acgccgccgg gtcattgacc acataggcgg aaaggcgtgt
     2821 ccggtcgatc tggccgtcgc gcagcgtgcc cggaaaagcc gcctcgacga gcggcacggc
     2881 cgcgccacga tagagcgcgt gcacggcggc atccgaatcg tgcacgggaa ttccagcttc
     2941 gcgaaaaatt gcggccgtca ccgatttgcc catgccgatc gagccggtga ggccgagaac
     3001 gaacatcagc ttttatcggc gatgtcggcg gcaacgcgcg cgcgtaagtc cggtgtcacc
     3061 tggggagtct gcccgaacca gcgcgcaaac cccggcaccg cctgatgcag caacatgccg
     3121 agaccacccg acgtgcgcag cccgcgtgct gacgcctggc gcagaagcgc ggtttcgagc
     3181 ggcgcataga cgatatcggc gacgacggcg cgtgccggca gcggcgcgag atcgatatcg
     3241 agtggcggct ggccgatcat gccgagcgat ttcgtattca ccagcaaatc ggcgtcgcgc
     3301 agaagggcgg gcagcacagc gaaatcagca aggtcgatcc gcgcatcagg aaattgcctt
     3361 tgcaattgtt cgccccggct gcgcgtccgg ttgacgatag cgatgtcacg gatgccgcgc
     3421 gcattcagcg cgaagacgat tccgcgcgcc gcgccgccag cacccagcac gacggcttta
     3481 tccccgctct tatcccagtc aggaacatca gcgtcgagcg aggcgagaaa gcccgcgaca
     3541 tcagtgttgt cgccgaaaag tttttcatcc tcgatccaca gggtattcac agcctggagc
     3601 tggcgcgcga ccggcgtcgt cacggcgcag gctgcgaagg
//
LOCUS       DASWOG010000142         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58232,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000142 DASWOG010000000
VERSION     DASWOG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58232"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1371
                     /locus_tag="VGB93_02875"
     CDS             322..1371
                     /locus_tag="VGB93_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0145103.1"
                     /translation="MPVPQHALQDNSAGSSVVLAAAAHGAVDFITARGGDPERVAAVA
                     GLDLRQLETPTDPIDLHGYCGLFEEAARQTKEDNFGLWYGQQFEPHMLGLIGFIALAS
                     PTLGEAVKNLAHYFPLHQSGTRTRLFAHDGLLHLEYQIVDGRIARRRQDAELTMGMFT
                     NVFRHCLGRGWAPEEVHLEHAAPQAPDAHEHAFHAPVFFGRGSNRLVFRDDQLDRPMQ
                     GADPQLLSLLRASLTRVAQTTQLAFKDRLTGEIRAHLAIGDVKFADIAAALKMSQSTL
                     LRHLEDENIGFARLVEQVRREQARAYLAQRHIAVSEIALLLGYSEISAFSRAFKRWEG
                     VSPNQYRATAQTQAL"
     gene            complement(1307..2242)
                     /locus_tag="VGB93_02880"
     CDS             complement(1307..2242)
                     /locus_tag="VGB93_02880"
                     /inference="COORDINATES: protein motif:HMM:NF017515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0145104.1"
                     /translation="MQIDSIFQILIQHDGQCPDVLPERIAKNVASVRRLYPTAKHHLF
                     GGDQIRAFLEDNFDPRILAAYDILLPYAFKADLARLCLLYKFGGLYVDLGVWMMEAPQ
                     IPADKAIVVFRDQYVPGGAAWAVSNGLIYAAPGRAELKLAIDLIVENVETRNYGADVQ
                     TISGPALFGRVFAMANDLAHYWIGDSRLVTFEGGDAPRMSFIAPDGRLIAWRAKSVDA
                     DLTHLGLAQTNNYSQIYDSHMAFGETTPAYAPLFARLPWRLELEIAKLLCTLGLKNDG
                     TYLWGRALTRRSRAAKEPASAPSRGTDWAKRPPSV"
     gene            complement(2294..2370)
                     /locus_tag="VGB93_02885"
     tRNA            complement(2294..2370)
                     /locus_tag="VGB93_02885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2334..2336),aa:Pro,seq:cgg)
BASE COUNT          470 a          728 c          743 g          489 t
ORIGIN      
        1 tgcagatgat cgaaaacgaa atcgcgaagt tcgaaaagct cttcatcccg cgcgcaagtt
       61 cctgcgcata gcccatctgg tgcagcactt gaacgtcttc ggcgagtacg gattggtcgg
      121 cggattcggg tgcggcggac atgaacgtct cctcggtgct ctcttgcgga gcgtacagct
      181 tcgactagac gaggcgcgcg atacgatttg ttggccggcc tcgactaagg tcctgttcag
      241 tctgacctgc ggggaaagat atgttcttga cattttcccc ctgagatttg accttcgtct
      301 ctccccagcc ggagaattga cgtgccggtc ccgcagcatg cccttcagga caacagcgct
      361 ggcagttcag tggtgctggc cgcggctgcg catggcgccg tcgatttcat caccgcgcgc
      421 ggcggcgatc cggagcgcgt cgcggcggtc gcgggcctcg atttgcggca attggaaacg
      481 cccaccgatc cgatcgatct gcacggctat tgcggcctct ttgaagaggc ggcgcgccag
      541 acgaaggaag acaatttcgg gctttggtac ggccagcaat tcgagccgca tatgctcggc
      601 ctcatcggct tcatcgcact cgcgtcgccg actttgggcg aagcggtcaa gaatctcgcc
      661 cattactttc cgctgcatca atccggcacg cgcacgcggc ttttcgcgca cgacggcctg
      721 ctacatctcg aatatcaaat cgtcgatgga cggatcgcac gtcggcgtca ggatgccgaa
      781 ttgacgatgg gtatgttcac caacgttttc cgccattgtc ttggccgcgg ctgggcgccg
      841 gaagaagtgc atttggaaca tgctgcaccg caagcgccgg acgcgcacga acatgcgttt
      901 catgcgcccg tctttttcgg ccgcggcagc aaccgcctgg tattccgcga cgatcagctc
      961 gaccggccga tgcagggcgc cgatccgcaa ttgctgtcgc ttttgcgcgc gagcctgacg
     1021 cgggttgcgc agacaacgca gcttgcgttc aaagatcggt tgaccggcga aatccgcgcc
     1081 catcttgcta tcggcgacgt gaagtttgcc gatatcgccg cggcgctgaa aatgtcgcaa
     1141 tcgacgctgt tgcggcacct cgaagacgag aacattggtt ttgcccggct tgtcgaacag
     1201 gtgcggcgcg aacaggcccg cgcctatctt gcgcaacgcc atattgcagt cagcgaaatc
     1261 gcgctgctgc ttggctattc ggaaatcagc gctttttcgc gcgcgttcaa acgctgggag
     1321 ggcgtttcgc ccaatcagta ccgcgcgacg gcgcagacgc aggctcttta gccgcccgac
     1381 tcctgcgcgt caacgcacgg ccccagaggt aggtcccgtc gttcttgagg ccgagcgtgc
     1441 agaggagttt ggcaatctcg agttcgagcc gccacggcaa tctcgcaaac aagggtgcat
     1501 aggccggcgt tgtttcgccg aaagccatgt gcgaatcgta aatctgcgaa taattgttgg
     1561 tttgcgcgag gccgagatgc gtcagatcgg cgtccaccga tttcgcccgc cacgcgatca
     1621 gcctcccgtc cggcgcgatg aatgacatgc ggggtgcatc tccgccctcg aatgtcacga
     1681 ggcgagaatc tccaatccaa taatgcgcga ggtcgttcgc catggcgaag acgcgcccga
     1741 atagcgccgg cccgctgatg gtttgcacat cggcgccgta attgcgggtc tcgacattct
     1801 cgacgatgag gtcgatcgcc agtttgagtt ccgcgcggcc gggcgccgca tatatcagcc
     1861 cgttggagac agcccaggcc gcgccgcccg gaacatattg atcgcggaaa acgacgatcg
     1921 ctttatccgc cggaatttgc ggcgcctcca tcatccagac gccgagatcg acataaagac
     1981 cgccgaattt atagagcagg caaagccgcg cgaggtcggc cttgaacgca tacggcaata
     2041 aaatgtcgta ggcggcgagg atacgcgggt cgaaattgtc ttcgaggaag gcgcggatct
     2101 gatcgccgcc aaatagatgg tgctttgccg tgggatagag ccggcggacg ctcgcgacgt
     2161 ttttcgcgat ccgctcgggc agcacgtctg ggcactggcc gtcatgctgg atcaggatct
     2221 ggaagatcga atcgatctgc atcggtcacc gactcatcgg gacgccgaag cgtcaaggcg
     2281 ggaggagcag aaatggtcgg agtgagagga ttcgaacctc cggccccctc gtcccgaacg
     2341 aggtgcgcta ccaggctgcg ctacactccg atttttgccg attttgcagg gcgagaaggc
     2401 ctgcggcctg tgccttatag ccataccgcc
//
LOCUS       DASWOG010000143         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58234,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000143 DASWOG010000000
VERSION     DASWOG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58234"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VGB93_02890"
     CDS             complement(<1..1346)
                     /locus_tag="VGB93_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0145105.1"
                     /translation="MPSFSRNLEQTLHRALAVANERHHEYATLEHLLLSLTEDTDAAA
                     VLRACSVDLEILRKNLRDYIDQELDNLVSDGREDAKPTAGFQRVIQRAVIHVQSSGRE
                     EVTGANVLVAIFAERESHAAYFLQEQDMTRYDAVNYISHGIAKRPGMSDSSKTPRGAD
                     DDDGRDGKDKESGDSKKKEGALDAYCVNLNRKAKDGRIDPLIGREAEVLRTIQVLCRR
                     HKNNPLLVGDPGVGKTAIAEGLARKIVKNEVPEVLADATVFALDMGTLLAGTRYRGDF
                     EERLKQVMKEIENHKKAILFIDEIHTVIGAGATSGGAMDASNLLKPALAQGTLRCIGS
                     TTYKEYRQYFEKDRALVRRFQKIDVNEPTIEDAIEIMKGLKPYFEEYHKVRYTNEAVK
                     AAVELSARYIHDRKLPDKAIDVIDESGASQMLVPEAKRKKTIGIKEIETTIATMAR"
     gene            complement(1362..1709)
                     /gene="clpS"
                     /locus_tag="VGB93_02895"
     CDS             complement(1362..1709)
                     /gene="clpS"
                     /locus_tag="VGB93_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY0145106.1"
                     /translation="MPSRAAKDPQKINDPGPGNGTAVITRTKPQTRRPNMYRVLLLND
                     DYTPMEFVVVVLRKYFNKGPEEATRIMLHVHQHGVGECGVFTYEVAETKVTQVMDYAR
                     KHQHPLQCIMEKK"
     gene            complement(2084..>2345)
                     /locus_tag="VGB93_02900"
     CDS             complement(2084..>2345)
                     /locus_tag="VGB93_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY0145107.1"
                     /translation="AETTDYSKRSLETSSAYIEKLVGAKTLDNAIQIQSEYAKVAYER
                     FVAQTSKISELYSNLAKEAFKPVESAIAKVQSAASSVTSAVK"
BASE COUNT          453 a          693 c          635 g          564 t
ORIGIN      
        1 atccgcgcca tggtcgcgat tgtcgtctcg atttccttga tgccaatcgt cttcttgcgt
       61 ttcgcttcag ggacaagcat ctggctcgcg ccggattcgt cgatcacatc gatcgctttg
      121 tccggcagtt tgcgatcgtg aatgtagcga gctgaaagct caaccgctgc tttcaccgcc
      181 tcgttggtgt aacgaacctt gtgatactct tcgaaatagg gcttcagccc cttcatgatt
      241 tcgatcgcat cctcgatcgt cggctcgttg acgtcgattt tctgaaagcg gcgaaccagc
      301 gcacggtcct tctcgaaata ttggcgatat tccttatagg tcgtcgagcc gatgcaacgc
      361 agcgtgcctt gcgccaaagc cggcttcagc agattcgacg catccatcgc gccaccggat
      421 gttgcaccgg caccgatgac agtatggatc tcgtcaatga atagaatcgc cttcttatga
      481 ttctcgatct ccttcatcac ctgcttcagg cgctcttcga aatcgccccg atagcgtgtg
      541 ccggcaagca gcgtgcccat gtcgagggcg aacactgttg cgtcggccag gacttccggc
      601 acttcgttct tgacgatctt ccgcgccagc ccttcggcga tggccgtctt gccgacacca
      661 ggatcgccga ccagcaacgg attgttcttg tggcggcggc agagcacctg gatcgtgcga
      721 agcacttcgg cctcgcggcc gataagcgga tcgatacggc cgtctttcgc tttgcgattg
      781 aggttgacgc aataggcatc gagagcgccc tcctttttct tggagtcgcc gctctcttta
      841 tccttcccgt cgcggccgtc atcgtcgtcg gcaccgcgcg gcgtctttga cgaatcggac
      901 ataccgggcc tcttcgcaat gccgtggctg atgtagttta ccgcgtcgta gcgggtcata
      961 tcctgctctt gcaggaaata ggcggcgtgg ctctcgcgct cggcgaaaat agccacaagc
     1021 acattcgcgc ccgtgacttc ctcgcggccc gaagactgga cgtgaatgac ggcgcgctga
     1081 atgacacgct gaaagccagc cgtaggcttg gcatcctcgc gaccatcgct gacaagattg
     1141 tcgagctcct ggtcgatgta atcacgtaaa ttcttccgca agatttcgag atcgaccgaa
     1201 catgcgcgta gaaccgccgc cgcgtcggta tcctccgtca aactcaggag aagatgctcc
     1261 agcgtcgcgt attcatgatg gcgctcgttg gcaacagcga gagcgcggtg aagagtctgc
     1321 tcgagattac gtgagaagga cggcatggaa actccgatct ctcatttctt ttccatgatg
     1381 cattgcagtg gatgctgatg cttcctggcg tagtccatca cttgggtgac tttagtctca
     1441 gcgacttcgt aagtgaaaac gccgcattcg ccgacgccat gctggtggac atggagcatg
     1501 atccgcgtcg cctcttccgg ccctttgttg aaatatttgc gtaggacgac cacgacaaac
     1561 tccatcggcg tgtaatcgtc gttgaggagc agcacgcgat acatattcgg gcgtcgcgtc
     1621 tgcggcttgg tgcgcgtaat gaccgcggtg ccgttccccg gcccgggatc attgattttt
     1681 tgtgggtcct tggctgccct tgacggcatg cctttgcctc ctgtcgcgca agcggcacgg
     1741 agcggccgga agctttccca accggcaact ccggtcaacg cgcaatcata actccggttc
     1801 gcaacgctcg cctaggcgaa actgcgccac gattgccact ctcagcaatt atcttgccgg
     1861 tccatgcaag atagatacaa aacacggcat ttccagaata gcatgtcatc acagcgataa
     1921 tttatttcgc tttcctatcg attcgcaatt tttcgccgcg cgcgagacaa ataaaaaggc
     1981 ccgacgtctg tcgagccttc attgcccttt gggggcttta gatgtcagcg cagctgcact
     2041 cgcagcggaa gacgcaatcc gcccgagtcg acccgcagag gtcttacttg accgccgacg
     2101 tcacgctgct cgctgcagac tggaccttgg caatcgcgct ctcgaccggc ttgaaagctt
     2161 ccttggcgag gttggaataa agctcgctga tcttgctcgt ctgggcgaca aagcgttcat
     2221 aagcgacttt ggcatattcc gactggattt gaatcgcgtt gtcgagcgtc ttggcgccca
     2281 caagcttctc gatataggcc gagctcgtct caagcgagcg cttggaatag tccgtcgttt
     2341 ccgct
//
LOCUS       DASWOG010000144         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_21831,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000144 DASWOG010000000
VERSION     DASWOG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21831"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VGB93_02905"
     CDS             <1..971
                     /locus_tag="VGB93_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0145108.1"
                     /translation="AYSATAPLLERRETQGKVRRCHGDVHLRNIVLENGKPILFDAIE
                     FDDLLATIDILYDLAFVLMDFWQRGLHAHANLLFNRYLWRAPEFEQELEGLAALPMFL
                     ALRAAIRARVVAALSDIADADHSAEAKSFFGASAAFLRPEPARLVAVGGLSGTGKSTL
                     ATALAGAIGRAPGAVHLRSDIERKRLRGADEYERLPPDAYAPAITAQVYGRLLNLAEI
                     ALRAGQSIIVDAVHAREDERVALAALAKKLDVPFTGLWLDAPVETLVARVEARQHDAS
                     DANAAVVRQQTALALGAIEWTRLDAGQGFDLLLARAQAALNLPAAE"
     gene            complement(949..1944)
                     /locus_tag="VGB93_02910"
                     /pseudo
     CDS             complement(949..1944)
                     /locus_tag="VGB93_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            2150..2461
                     /locus_tag="VGB93_02915"
     CDS             2150..2461
                     /locus_tag="VGB93_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY0145109.1"
                     /translation="METQAISSEIQNAQSSLFSATAPLHDVWRQTWIEWPVALASESL
                     RFAAHRLRAHSDFYGKIQNCGSVPEFIEAQSKFVRSAFDDYGTEATKVIKDATQHTPL
                     S"
BASE COUNT          522 a          800 c          823 g          517 t
ORIGIN      
        1 cggcctattc cgctaccgcc ccattgctcg aacgtcgcga aacgcagggt aaggtgcggc
       61 gctgccatgg tgacgttcac ctgcgcaata tcgttctcga aaacggcaag ccgatcctgt
      121 tcgacgccat cgaattcgac gatctgctgg cgacgatcga catcctctac gatctcgcct
      181 tcgtcctgat ggacttctgg caacgcggcc tccacgccca tgccaatctc ttgttcaacc
      241 ggtatttgtg gcgcgcgcct gaatttgagc aggagcttga gggattggcc gcgctgccga
      301 tgtttttggc gttgcgcgcg gcgatccgcg cgcgcgtcgt cgcggcgctt tccgacatcg
      361 ccgacgcaga tcacagcgcc gaagcgaaga gctttttcgg cgcttcggcc gcgttcctgc
      421 gtcctgagcc ggcccggctc gtggcagtcg gcggtctgtc ggggaccggc aagagcacgc
      481 tcgcaacggc gcttgccggc gccatcggcc gggcgccggg cgcggtgcat ctacgcagcg
      541 acatcgagcg caagcgcctt cgcggcgccg acgaatacga aagattgccc ccggacgcct
      601 acgcgcccgc gatcaccgcg caggtttatg gccgcctgct caatctggct gaaatcgcgc
      661 tgcgcgccgg ccaaagcatc atcgtcgatg ctgttcatgc cagagaggac gagcgcgtcg
      721 ctttggcagc gctcgccaaa aaactcgacg tgccttttac gggcctgtgg ctcgatgcgc
      781 cggtcgaaac gcttgtcgcg cgtgttgaag cgcgccagca cgatgcttcc gacgccaatg
      841 cagccgtcgt ccggcagcag acggcattgg cgttgggcgc aattgaatgg acgcggctcg
      901 atgccggcca gggtttcgat ctcctgcttg cgcgcgcgca ggccgcgctc aatcttcccg
      961 cggcggaata agcaccgcag cgccgtgcaa gcgaccacgc ttcaaatcgg ccagggcttc
     1021 attcgcccgg tcgagcgggt agggcgtcgt gtaggttttg atatcggcgc ccgcagcgat
     1081 cttcaggaag tcgatgccat cctgacgcgt cagattggcg accgagaaaa gcttccgttc
     1141 ttcccagagg aggtcgtagg gaaattcggg aatgttgctc atgtgaatgc cggcgcagac
     1201 gacagtgccg cctttcttca ccgccgccag cgccaaaggc acgagcgggc cgatcggtgc
     1261 atagatgatc gccgcatcta gtttctccgg cggcggctga tcagagccgc cagcccaaac
     1321 agcgcccagg ctcagcgcga agtcttatgt cccacggtcg ccgggactgg taaaggcgaa
     1381 tatcttgcgg ccgagtttta tcacgacctg cgcgataata tgcgccgccg cgccgaaacc
     1441 gtagaggcca agcgttttgc cttcgcccgc catgacgaag gagcgccagc cgatcagccc
     1501 ggcgcagagc aggggcgcga gttcctgatc cgacccacgt tcgccgagcg gaaacgtgta
     1561 atgcgcatcg gcaatcgcat gcgtggcaaa gccgccgttg cgcgtatagc cggtgaagat
     1621 cggataatcg cagagatttt cgtggccgct cagacaaaac gggcaatggc cgcacgtatg
     1681 gccaagccaa ggaatgccaa cgcgctcgcc aagcctcaga tgagtgacat cagggccgat
     1741 cgcatcgatc cgtccgacga tctcatgtcc cggtacgatc ggcactttcg gatcaggcaa
     1801 ttcgccgtca acgacatgga gatcggtgcg gcagacgcca caagcgctga cgcgaacgcg
     1861 aatttcgccg cggccaggca cagggtccgg tagctcttca tagataagcg gtgcaccgat
     1921 ttggtggaga accatagctt tcatgtatca ccgggaattg tcgccaaaat ctgcgagggt
     1981 gaatttttcg tcacatgcgt tgaatatgct ttcgtcggcc gagttaagtt cttaggctac
     2041 gaaaattcaa ggacgcagcg agggctgggc tgctgcatga cgatgccggt ttggcatcgc
     2101 cgaatctgca aatccaaacg tgcgaggcgt cgcctcggtg gaggacgtca tggaaacgca
     2161 agccattagc tctgaaatac aaaacgcgca gagcagtctt ttttcggcga cggcgccgct
     2221 gcatgacgtg tggcgtcaga cgtggattga atggccagtt gctctggctt cggaatcctt
     2281 gcgtttcgcc gcgcatcgcc tgcgcgcgca cagcgatttc tacggcaaga tccagaattg
     2341 cggctccgtg ccggaattca tcgaggcgca atccaagttc gtgcgcagcg ccttcgatga
     2401 ttatggaacg gaagcgacca aggtcatcaa agacgcgact cagcatacgc cactctcttg
     2461 atgcgtcggg cgcgttgccg gccacggact tgacggcgtc cggggcgcgg tgactgaagt
     2521 ccgccgggcc tgactggcgg gcaatgctga ttttggtcta ataaattgaa ttccttcgcg
     2581 ttgatcgctt cctgcctcag cgaaaaagac aagtttggcg agagaggtga atcaaacggg
     2641 catgtgcatg cgccgaggat tg
//
LOCUS       DASWOG010000145         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_315197,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000145 DASWOG010000000
VERSION     DASWOG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315197"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VGB93_02920"
     CDS             <1..1031
                     /locus_tag="VGB93_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0145110.1"
                     /translation="DHYRTRLTHSIEVGQIARALARALGLDDDLAEALALGHDLGHTP
                     FGHTGEDALDEAMRPYGGFDHNAQAMRIVTKLEHRYASFDGLNLTWETLEGLVKHNGP
                     LLGADGRPIARYVGCGLPAAILEYATMHDLRLDLFAGPEAQAAAIADDIAYNAHDIDD
                     GLRAGLFDFSQLAEVDYLRERIEQIDVLHPGLERQRSIHELVRRVITDFIEDAINESA
                     ARLKASRVVSADEVRALDHAIAGFSPVMAAHQQTIKSFLFQNMYRHPDIKRIRVEAAG
                     VARDLFDIFFKDPELMPPEWASLAITRGAEDEAKMARVVCDYIAGMTDRYALAEHQRL
                     FDETPELR"
     gene            1058..>2394
                     /locus_tag="VGB93_02925"
     CDS             1058..>2394
                     /locus_tag="VGB93_02925"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0145111.1"
                     /translation="MNIYSDFHSRVVTLLQNIIASGRLPAGLDLARFVVEPPRDPAHG
                     DLSVNAAMVYAKEAKAHFANPRQLSTELAMSLADDPDVDQAEVAGPGFLNIRVKPQVF
                     AKILSAVLTQGDRFGAATAGAAEKIDVEYVSANPTGPMHVGHGRGAVFGDALASLLAF
                     AGYDVTREYYINDAGAQVDVLARSAYLRYREALGETITIPEGLYPGDYLKPIGEALAT
                     EHGRALLDEPEGVWLPKVRAAANAGMMEMIRADLAALHIAHDVFFSERSLTQGGRDEI
                     AAAIDELRAANLVYVGRLPAPKGQPSEDWEDREQELFKSTSFGDDVDRPLKKSDGSYT
                     YFAADIAYHKTKIDRGFRNLIDVWGADHGGYMKRMQAAVTALSGGKAILDVKLCQLVK
                     LMRNGEPVKMSKRSGDFVTLREVVDEVGVDAVRFMMLFRKNDAPLEFDLAKVI"
BASE COUNT          456 a          728 c          737 g          473 t
ORIGIN      
        1 gcgatcatta ccgcacacga ctcacccatt cgatcgaggt tggccagatt gcccgggctc
       61 tcgcccgcgc gcttggcctc gatgacgatc tggccgaggc cctcgcgctg ggccacgacc
      121 ttggccatac gcccttcgga cataccggcg aagatgccct cgacgaggcg atgcggcctt
      181 atggcggttt cgaccataat gcccaggcaa tgcgcatcgt caccaagctt gaacaccgct
      241 atgcatcttt cgacgggctc aacctgacct gggaaacact cgaaggcctg gtcaagcaca
      301 atgggccgct gctcggggca gatggacgcc cgatagcacg ctatgtgggg tgcggcctgc
      361 cggccgccat cctcgaatat gcgacgatgc atgatctgcg gctcgatctc tttgccgggc
      421 cggaagcgca ggcggcggcc atcgccgacg acattgccta caacgcgcat gacatcgatg
      481 acggtttgcg cgccggcctg ttcgattttt cgcaattggc cgaggtcgat tatctgcgcg
      541 agcgcattga gcagatcgat gtgcttcatc ccggattgga gcgccagcgg agtatccacg
      601 aactcgtgcg gcgcgtgatt accgatttca ttgaagacgc aatcaatgag agcgcggcac
      661 ggctgaaggc gagccgcgtt gtgagcgccg acgaagtacg cgcgcttgac catgcgatcg
      721 ccggcttttc gccggtgatg gcggcgcacc agcagacgat caagagtttc ctgtttcaga
      781 atatgtaccg ccatcccgac atcaaacgca tccgggtcga ggccgctggc gtcgcgcgcg
      841 atctcttcga tattttcttc aaggatccgg agctaatgcc gccggagtgg gcgtcgctgg
      901 caatcactcg cggcgccgag gacgaagcca aaatggcgcg cgtcgtctgc gactatattg
      961 ctggcatgac cgaccgctac gcgcttgccg agcatcagcg gctttttgac gagacgcccg
     1021 aattgcgtta gcgcgctaca aacaccaaag caaagccatg aacatttatt ccgacttcca
     1081 cagccgcgtt gtgacgctgc tgcaaaacat catcgccagc ggccggctgc ccgccggtct
     1141 cgatcttgcg cgtttcgtcg tcgagccgcc gcgcgatccc gcgcatggcg atctctcggt
     1201 caatgcggcg atggtttatg cgaaggaggc gaaagcgcat ttcgccaatc cgcgccaact
     1261 ctcgaccgag cttgcgatgt cgctggctga cgatcccgac gtggatcagg cggaagtcgc
     1321 ggggccgggc tttctcaaca ttcgggtcaa gccgcaggtc ttcgcgaaga ttctgagcgc
     1381 cgtgctgact caaggcgacc gctttggcgc ggcgacagca ggcgctgctg aaaagatcga
     1441 tgtggaatat gtgtcggcca atccgaccgg gccgatgcac gtcggccacg gccgcggcgc
     1501 cgtcttcggc gatgcgctcg ccagtctgct cgccttcgcc ggctacgatg tgacgcgcga
     1561 atattacatc aacgatgccg gcgcgcaggt cgatgtgctc gcccgctccg cctatctgcg
     1621 ttatcgcgag gcgctcggcg aaacgattac gattcccgag ggactttatc cgggcgatta
     1681 tctcaagccg atcggtgagg cattggcgac cgaacacggc cgcgcgcttc tcgatgagcc
     1741 ggaaggtgtt tggctgccga aggtacgtgc ggctgcgaac gccggcatga tggagatgat
     1801 ccgcgctgat cttgccgcgc tccatatcgc gcatgatgtc ttcttttccg agcgaagtct
     1861 gacgcagggc ggccgcgacg agatcgcagc ggccattgat gaactgcgcg ccgcgaacct
     1921 cgtctatgtc gggcgtctgc cagcaccgaa aggtcagcct tccgaggatt gggaagaccg
     1981 cgagcaggag cttttcaaat cgacatcttt tggcgatgac gttgaccggc cgctgaagaa
     2041 atccgacggc agctacacgt atttcgccgc cgatatcgcc tatcacaaga cgaagatcga
     2101 tcgtggcttt cgcaatctga tcgacgtttg gggcgccgat cacggcggct acatgaagcg
     2161 gatgcaggcc gcggtgacgg cgctgtccgg cggcaaggcg attctcgacg tcaaactctg
     2221 ccaactcgtt aagctgatgc gcaatggcga gccggtgaaa atgtcgaagc gctctggcga
     2281 tttcgtcacg ctgcgcgagg tcgtcgatga ggtcggcgtc gatgccgtgc gcttcatgat
     2341 gctcttccgc aagaacgatg cgccgcttga attcgatctc gccaaggtca tcga
//
LOCUS       DASWOG010000146         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58308,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000146 DASWOG010000000
VERSION     DASWOG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58308"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="tmk"
                     /locus_tag="VGB93_02930"
     CDS             complement(<1..638)
                     /gene="tmk"
                     /locus_tag="VGB93_02930"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0145112.1"
                     /translation="MDILHPSARKRGTFITLEGGEGVGKSTQMRHLITRLQARGIEAI
                     STREPGGSPGAEILRDLLLSGMIKDLGPKAEAIIFAAARIDHIDHTIAPALAANKWVV
                     CDRFADSTRAYQGALGGLDPGFLRALERVTVGDTRPDLTLILDLPPEIGLARAAARRG
                     AGQASDRFEGEGLAFHEALRRAYLAIAAAEPERCVLVDADHSEEEVAQAIWDT"
     gene            complement(641..1849)
                     /locus_tag="VGB93_02935"
     CDS             complement(641..1849)
                     /locus_tag="VGB93_02935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0145113.1"
                     /translation="MNSDEALGIRLARLPALALAALALTLLAVTPGRAEQPFSTSVPS
                     AILIDADSGAVLFEKNADTPQIPASTAKIMTLELIFEALGGGHLKLTDTLNVSENAWR
                     TGGAMARGSTMFAALHSNVSIEDLIRGIAVVSANDASIVLAEGTAGSEGAFATRMTER
                     AHELGLAHSTFTNPWGQDDPDQRTTARDMATLAAYMIGTYPQYYHYFAEKDFTWNKIK
                     QPNRNPLLEMDLGADGLKTGNIGDSGYGIVASAVQNGQRLILAIYGSKTAKERETEAA
                     RIFQWGFRSFAAKTLFKAGEVIGYADVYGGSTGYVPLVSEKPVRLLIPRESDEKLRGQ
                     IVYTGPLVAPVASGREIAQLRVFRGASEILDTPLKTAANVGVGSLQRRAMDAGIEYTE
                     EMFRKYVLKK"
     gene            complement(1946..2770)
                     /locus_tag="VGB93_02940"
     CDS             complement(1946..2770)
                     /locus_tag="VGB93_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0145114.1"
                     /translation="MFVASLAAAGFAALGLGGCAQQQQVAHSSEYFPQSVYGEASPRV
                     VADGRDVPRGGGKYLVGHPYMVAGRMYYPSERKHYTAVGYASWYGDAFHGRRTANGEI
                     FDRNSISAAHPTMPLPSYARVTNLRNHHSMIVRVNDRGPFAPGRIMDVSRRVADLLDF
                     RRTGTTMVKVEYVGRASLEGSNDEKLASTLRIDDQSAPAAPPIMLADADADAPVQTAS
                     ADPDDVPQTSAPAPDGGSETADASPSHVFIGTAPLPPSRPYDLDTIPGADVPIASQ"
     gene            3302..4756
                     /locus_tag="VGB93_02945"
     CDS             3302..4756
                     /locus_tag="VGB93_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0145115.1"
                     /translation="MSKSVFPMGFVIALLTSASAFAADLPSYKSAPAFVPPPPVMSWT
                     GLYLDAQGGYGGWNANTSTPTAGVNQTAGGRGFLGRVGAGFDYQFSDRFVAGVLADYS
                     YGRQTGIIQDSGVGLSGNTNQNWSWAAGGRLGYLFTPDLLAYGEAGFTQAHFTGTTML
                     NSFTAAPSGDGTPGLTASGWFVGAGFETMFAPGWFFRSEYRYADYGSHTLADTGALPL
                     DGIRFHPVEQTITSGIVYKFGYPEFTAPGIPSLGMLTDGFAPAPAQTNWTGFYADAGI
                     GYGLWSANSATENPATGNCIICNTQRFGGRGVFGTAGAGYDYQLTNKFVGGVFGDYDP
                     SDMRGTIDDQANGFVGSISQQWAWGVGARLGYLVSPAVLTYTKAGFTQARFGGTNLDT
                     FGGAGTGTFTPGFTTNGYFIGGGVEAEFAPGWFWRAEYRYASYGSRELAELGVGGAAA
                     SEIHFRPEEQAATVGLVYKFNWTPSVPAPLAAKY"
     gene            complement(4858..>5094)
                     /locus_tag="VGB93_02950"
     CDS             complement(4858..>5094)
                     /locus_tag="VGB93_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145116.1"
                     /translation="IRNWGTFSKAEFEAEFIGDPAHTRHKDLVALLQAPEFSHPIARV
                     GNILGYTKTPPAMSSEIMFDDGADTMTDAILEVD"
BASE COUNT          962 a         1561 c         1546 g         1025 t
ORIGIN      
        1 gtatcccaga tcgcctgcgc aacttcctct tcactgtggt ccgcatccac aaggacgcac
       61 cgctcgggct ctgcggcggc gatcgcgagg taggccctgc gcaaagcttc gtggaaggcg
      121 agcccttcgc cttcaaaccg atcggacgcc tgccccgcac cacgccgcgc ggcggcccgt
      181 gccagcccga tctcgggcgg cagatcgagg atcagggtca gatcggggcg cgtgtcgccg
      241 acggtcacgc gctcgagcgc gcgcagaaag cccggatcga ggccaccgag cgcgccttga
      301 taggcgcgcg tcgaatcggc gaagcggtcg cagacgaccc atttgttggc cgcaagcgcc
      361 ggcgcgatcg tatggtcgat atggtcgatc cgcgcggccg cgaagatgat cgcctcggcc
      421 ttcggaccaa ggtccttgat catgcctgat agcaaaagat cgcgcagaat ctcggcgccc
      481 ggcgagccgc ccggctcacg cgttgaaatc gcctcgatgc cgcgcgcctg cagccgcgtg
      541 atgaggtggc gcatttgcgt cgacttgccg acgccctcgc cgccttccag cgtaatgaac
      601 gtaccgcgtt tgcgcgcgga cgggtgtaga atgtccaagg tcattttttc aaaacgtatt
      661 tgcggaacat ttcttcggta tattcgattc ccgcatccat cgcgcgtcgc tgcagcgagc
      721 cgacaccgac attcgccgca gtcttcaaag gcgtatccaa aatctcgctg gcgccacgga
      781 agacgcgcaa ttgcgcgatt tcgcggccac tggcaacagg cgcgacaagc ggccctgtat
      841 acacgatctg gccacgcagc ttttcgtccg actcacgtgg aatgaggagc cgcaccggct
      901 tctcggagac gagtggcaca taacccgtgc taccgccata gacatcggca tagccaatga
      961 cctcccccgc cttgaacagc gttttcgctg cgaaagaccg aaatccccat tggaaaatcc
     1021 gcgccgcttc ggtctcgcgc tccttggcgg tcttggatcc atagatcgcg aggataagcc
     1081 gctggccgtt ttgaaccgcg gaggcgacaa tgccatagcc gctatcgccg atatttccgg
     1141 tcttcaaccc gtccgctcca aggtccatct ccagcagcgg attacgattg ggttgcttga
     1201 tcttgttcca ggtgaaatcc ttctccgcga agtaatgata atattgtggg taggtaccga
     1261 tcatgtaagc cgcgagcgtc gccatatcgc gcgccgtggt ccgctgatcg ggatcatcct
     1321 gtccccaggg attcgtgaag gtcgagtggg ccaagccaag ctcatgcgca cgctcggtca
     1381 tccgtgtcgc gaatgcacct tctgatcccg ccgtaccttc ggcgagcacg atcgacgcgt
     1441 cgttggcgga cacgacagcg atgccgcgga tcagatcttc gatgctgaca ttgctgtgga
     1501 gagccgcgaa catggttgaa ccgcgcgcca tcgcgccacc cgtccgccag gcattttcgc
     1561 tgacattcaa tgtatcggtc agcttaagat gaccgccgcc cagagcctcg aatatgagtt
     1621 cgagcgtcat gattttggcg gtcgaggccg ggatttgcgg cgtgtcagcg ttcttctcga
     1681 acaggacggc cccagagtcg gcatcaatca ggattgcgga ggggacactt gtgctgaagg
     1741 gttgctctgc tcgccccggt gtgacagcca gcagagttaa cgcgagtgcg gcaagggcca
     1801 gtgccggcaa tctcgcaagc cggatgccca aagcctcgtc gctattcatg ctcatctctg
     1861 aggaattccg cgccgcagca tgcatgtatg caagatgcgt ccgccgccgc cggaaatcaa
     1921 cgcgacggcc gcctgcttta cacgattact gtgaggcgat tggaacgtcc gcgcccggga
     1981 tcgtatcgag atcataggga cgcgagggcg gcagtggcgc cgtgccgatg aaaacgtggc
     2041 tcggcgatgc gtctgccgtt tcagaaccgc cgtcgggcgc gggcgcggag gtttgcggca
     2101 cgtcgtcagg atcggcggaa gcggtctgaa cgggtgcgtc ggcatctgcg tcagcaagca
     2161 tgatgggcgg cgcggcgggt gccgactggt catcgatgcg caacgtcgag gcaagcttct
     2221 cgtcgttcga gccttcaagg cttgcgcggc cgacgtattc caccttcacc atcgtcgtgc
     2281 cggtgcggcg gaaatcgagc aggtcggcaa cgcggcgcga aacgtccatg atgcggcccg
     2341 gcgcgaaagg accgcggtcg ttgacgcgga cgatcatcga atgatgattg cgcagattgg
     2401 tgacgcgcgc atagctcggc agcggcatcg tcggatgcgc agccgaaatc gaattgcggt
     2461 cgaagatttc gccgttcgcc gtgcggcggc catgaaacgc gtcgccgtac caggaggcgt
     2521 aaccgacagc ggtgtagtgc ttacgctcgc tcggataata cattcgcccc gcgaccatat
     2581 agggatggcc gacgagatat ttgccgccgc cccgcgggac gtctcggccg tcagccacga
     2641 cgcgcgggct cgcttcgcca taaacggact gcggaaaata ttccgaggaa tgagcaacct
     2701 gctgctgctg tgcgcagccg ccaaggccga gagcggcgaa tccggccgcg gccagagacg
     2761 ccacgaacac cggtttgcaa cctgcccggt cttgaacgtc ggaccgcact tcgtcacaga
     2821 cgaaacacga ccagaacgat aagcttttca accgcaaatc ccccgtactc cgctgccgat
     2881 ttagacctcg tcccccagag acgaagttgc accgctcgcc ttgctaaaac tttaaatgtt
     2941 ttgtcgacga agcgtccgga cgcggtaaac agaccggcaa atgggcctag agattcagac
     3001 aaaattgcgg caacgccgac aatgttgatc gcgcgccggg cttaaaatca tggaagttca
     3061 gcgagttgcc acatttcaaa gcggccgcaa atgcgcggga tatcttggaa aattcatatt
     3121 cacgaatctt cgttaacgac aatccgaatt tattgcgggc tcatgtagac ggtcatgcgc
     3181 ggggcgaaat ccagccgtcg tgtcttaggg gtgacaggtt ccgcgacgca gttgcggtag
     3241 gttacgccaa cgtttggtcg aatcggttgg tgtgacgctg cggtgcgtca aagggggcaa
     3301 aatgagtaag tcggtatttc caatgggctt cgttattgcc ctgctgacct cagcgtccgc
     3361 ctttgcggcc gacctgccct cctacaagtc agcgcctgct ttcgtgcccc cgccgccggt
     3421 catgtcctgg acgggtctct atctcgacgc gcagggcggc tacggtggct ggaatgcgaa
     3481 tacgtcaacg ccgaccgcgg gcgtcaatca gaccgcgggc ggccgcggct ttctcggccg
     3541 tgtcggcgcc ggtttcgatt atcagttcag cgaccgtttc gtcgctggcg tcttggcaga
     3601 ttacagctac ggccgtcaga ccgggatcat tcaggactcg ggcgtaggcc tctccggcaa
     3661 cacaaatcag aattggtcct gggcggccgg cggccgtctt ggctacctgt ttacgccaga
     3721 tcttctcgcc tatggcgagg ctggcttcac ccaggcgcat ttcaccggca cgacgatgct
     3781 taattcgttc accgcggccc caagcggcga cgggacaccg gggctcactg cgagcggctg
     3841 gtttgtgggg gcgggcttcg agacgatgtt cgccccgggg tggttcttcc gcagcgaata
     3901 tcgctatgcc gattacggtt cgcataccct cgccgacacc ggcgcactcc cgcttgatgg
     3961 aatccgtttt catccggtcg agcagacgat cacgtctggt atcgtctaca agtttgggta
     4021 ccccgaattc accgcgccag gcattccttc gttgggaatg ctgactgatg gtttcgcccc
     4081 ggccccagcc caaacgaact ggaccggctt ctatgctgac gccggcatcg gctacggcct
     4141 ctggagcgcc aattcggcaa cagaaaatcc cgcgacgggc aattgcatca tctgcaacac
     4201 gcaacgcttc ggcgggcgcg gcgtcttcgg cactgccggc gcgggctatg attatcagct
     4261 caccaacaag ttcgtcggtg gcgtcttcgg cgattacgac ccgtccgaca tgcgcggcac
     4321 gatcgacgat caggccaacg gtttcgtggg ttcgatctct caacaatggg cctggggcgt
     4381 cggtgcccgg ctcggttatc tcgtgtcgcc tgccgtgctg acctacacca aggctggctt
     4441 cacccaggcg cggttcggcg ggacgaacct cgatactttc ggcggcgccg gcactggaac
     4501 ctttacgccc ggcttcacca cgaacggtta ctttatcggt ggcggcgtcg aagccgagtt
     4561 cgcgcccggc tggttctggc gcgctgaata tcgctacgcc tcttacggct cgcgcgaact
     4621 tgctgaattg ggtgttggcg gagctgccgc gagcgaaatt cacttccggc cggaagagca
     4681 agctgcgacg gtgggccttg tctacaagtt caattggacc ccgtcggtgc ctgccccact
     4741 cgcagcgaaa tattgagcct gcgagactca atataccgaa cctggatctt taactgcgcg
     4801 gcgcttgagt gatctcaggc gccgcgttag tatgaattct ataccgttcg tctgtcgtca
     4861 atcgacttca agaatggcgt cggtcatcgt gtctgcgcca tcgtcgaaca tgatttctga
     4921 cgacatcgcc ggcggcgtct tcgtatagcc aagaatattg ccgacccgcg cgatgggatg
     4981 cgaaaactcc ggcgcttgga gtagtgcgac gagatccttg tgtcgcgtgt gcgccggatc
     5041 gccgatgaac tccgcctcga attcggcttt gctgaacgtt ccccaattgc ggat
//
LOCUS       DASWOG010000147         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_186929,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000147 DASWOG010000000
VERSION     DASWOG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186929"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..962)
                     /gene="hemB"
                     /locus_tag="VGB93_02955"
                     /pseudo
     CDS             complement(1..962)
                     /gene="hemB"
                     /locus_tag="VGB93_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176290.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
     gene            1092..2153
                     /locus_tag="VGB93_02960"
     CDS             1092..2153
                     /locus_tag="VGB93_02960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0145117.1"
                     /translation="MSEPEIISAKIGHCGLITLNRPRALNALTHDMVSAMAAALDDFA
                     QDSAIHTVAVRSSSPRAFCAGADIRRVHEHGKEGRHDAQLHFLRDEFRNCYRVNTYSK
                     PYVALIDGIVMGGGAGISIHGAHRVVGDSVTFAMPETAIGFFPDVGASFFLPRLPDRF
                     GTYLAMTAARISLGDVLALGLANAHVPSARFDILVEKFAAGEDAAKAIASESVAPPPS
                     DILKEREIVSRAFAFSGAKRIFTALADAAHRGSEFAEAALETLYSRSPSSVAIALRQM
                     QLGATLDLAQALNLEFRIAARILRSHDFYEGVRVTLVDKGDIPDWRPAIIDDVRAEEI
                     DTYFAALGDQELMLPAGLI"
     gene            2150..2446
                     /locus_tag="VGB93_02965"
     CDS             2150..2446
                     /locus_tag="VGB93_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145118.1"
                     /translation="MKLFMRREREEPVEPGAAIKLGVKAPEAAAQAQWGLLLVIFMRV
                     LAALWICQGLAEWALVLVPEKSVLEALPAAMAAAVIFFAIANLVAAVGLWLATQ"
BASE COUNT          422 a          742 c          794 g          536 t
ORIGIN      
        1 aaggcgatga ggctttccat catcgctcgg tcgccgtcga gccagccatt ctgcgccgca
       61 gccatgatca tcgaatattc gcctgatact tggtagacga aggtcggcgc gccggtattc
      121 tctttcacgc gcgcgagaat atcgagatag ggcaggccgg gtttgacgat cagcatatcg
      181 gcgccttcct agagatcgag cgcgacttcg cgcagcgcct cgtcggaatt cgccgggtcc
      241 atctgatagg tgcgcttgtc gccggcgagc gtcacattgc tgccgacggc ttcgcggaac
      301 ggcccgtaga aggccgaggc atatttcgcc gcataggcca tgatctgaac attctcgaag
      361 ccttctgcgt cgagcgccgc gcggatggtg ccgacccgcc cgtccatcat gtcggaaggg
      421 gcaatgatgt cggcaccggc acgcgcctgg ttcagcgcct gcgccaccaa cataccgacg
      481 gttgggtcgt tgacgatctc gtcgccgcgc aaaatgccgt catgcccgtg gctcgtatag
      541 ggatcgagtg cgacatcggt gatgatgccg atctccggca cttcccgctt gatggcgcgg
      601 gtcgcgcggc agacgaggtt tttctcgttc aaggcctcgc tgccgttggc atcgcgcaac
      661 gcgggatcgg tattcggaaa cagcgccagc gccgggatgc cgagtttggc ggccgcctcc
      721 gcctcacgca ccgcgacatc gacggaaaac cgttcgacgc cgggcatcga ggcgactttt
      781 tcggacttct ccgtgccctc gaccagaaaa atcggccaga tcagatcgtt gacgctgagt
      841 tcatgttctc gcaccaagcg gcgtgaccat tccgccttgc ggttgcggcg cagccgcacg
      901 gcgaggttga gcttcgtcgc cagcgggtcg agttcgggct tcgggtgcag cggcgcggac
      961 atgctaaact ccttgaattc gtgccatgct ttatcacggc cgagctttgt ggtgcgaatt
     1021 tcttagccgc atctcgcgta tggcggctct gcacattgac gtggcggcgt cgggccagtt
     1081 aatcgggcgc catgagcgag cccgagatca tcagcgcgaa aatcggccat tgcggcctca
     1141 tcacgctcaa tcgtccgcgc gcgctcaatg ccttgacgca tgacatggtg agcgcgatgg
     1201 ccgccgctct cgacgatttt gcgcaggatt ccgcaattca caccgtcgcg gtccgtagca
     1261 gcagtccgcg cgccttctgt gctggcgccg acattcgtcg tgtgcatgag cacggcaaag
     1321 aaggccgcca tgacgcgcag cttcacttcc tgcgcgacga atttcgcaac tgctatcgcg
     1381 tcaacaccta ttccaagccc tatgtcgcat tgatcgacgg cattgtgatg ggcggcggcg
     1441 cggggatttc gattcatggc gcgcatcgtg tcgtcggcga cagtgtgacc ttcgccatgc
     1501 cggagacggc gatcggtttt ttcccggatg tcggcgcgag cttttttctg ccgcgtctgc
     1561 cggatcggtt cggtacgtat ctcgcgatga ccgcagcacg tatcagcctc ggtgatgtgc
     1621 tggcgcttgg cctcgccaac gcgcatgtgc cgtcggcgcg tttcgatatt ctcgtcgaaa
     1681 aattcgcggc cggcgaagat gcggcgaaag cgatcgcgtc ggaaagtgtc gcgccaccgc
     1741 cttcggacat attgaaagag cgtgagattg tctcgcgcgc gttcgccttc tccggcgcta
     1801 agcgaatctt caccgcgctt gccgacgccg cgcatcgcgg ctcggagttt gcggaagccg
     1861 cactggagac tttatactcg cgctcgccgt cgagcgttgc gattgcgttg cggcagatgc
     1921 agcttggtgc gacgcttgat ctcgcgcagg cattgaatct cgagtttagg attgccgcgc
     1981 gaattttgcg gagtcatgat ttctacgagg gcgttcgcgt gacgctcgtg gacaagggcg
     2041 acattcccga ttggcgtccc gcaatcatcg acgatgtgcg ggcggaagag atcgacactt
     2101 atttcgcggc gctcggcgac caagaactca tgcttccggc aggtttgata tgaaattgtt
     2161 tatgcgccgc gaacgtgaag agccggttga accgggcgcg gcgatcaagc tgggcgttaa
     2221 agcaccggaa gcggcggcgc aggcgcaatg gggcctgctg ctcgtgattt tcatgcgggt
     2281 gctcgcggcg ctctggattt gtcagggtct cgcggaatgg gcgcttgtgc tcgtgccgga
     2341 gaaatccgta ctcgaggctc tgcctgcggc gatggcggcg gcggtgatct ttttcgcgat
     2401 cgccaatctc gtcgcagccg tcggtctctg gctcgcaacg cagtgaggcg gtgcgctgtg
     2461 gctgtttgca gcaagcgcgc aaattatcat cgcg
//
LOCUS       DASWOG010000148        11231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_21936,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000148 DASWOG010000000
VERSION     DASWOG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21936"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2651
                     /locus_tag="VGB93_02970"
     CDS             <1..2651
                     /locus_tag="VGB93_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0145119.1"
                     /translation="GGRIRQVMVDIDPAALAAKGITPLDVSNAINTQNLSLPMGDVKF
                     GDKDYDVRINSLADAVSILDDVPIKKVNGALIRIKDVAEVRDGYAIQQNIVRSEGRRS
                     VLLSILKAGNASTLDVVDRVKNDVLPVSRAAAPPGMKIEELFDQSIFVRSAITGVAVE
                     ATIAALLTSAMILLFLSSWRSTLIVAVSIPLSILSSIAILSAIGETLNIMTLGGLALA
                     VGILVDDATVTIENIHRLRQLGEKLPEATLHGAAGIALPTLVSTLAISAVFVSVEFLV
                     GPSKFLFTPMALAVVFAMLASYILSRTLVPIMAGLLLSGEHHGDEAHDDGRQPSIFTR
                     FSRGFEARFERLRDNYSVTLEKLIESNWKVPVAAVAVLAIGAVLYTQIGRDFFPAVDA
                     GEFKLHVRAPSGTRIETTEHIFENVENVIRRVIPDSERELLIDDIGVPFRYALPFDDG
                     TTVGGNDGQILVSLKEGHKPTAAYIKKLRDVLAREFPDMIFYFQPGDIVTQILNFGLP
                     APIDVRVVGYDKGNNLRIVQAMLDEIKHVVGVADAHLHQIVDAPELTVAIDRQRAQDL
                     GLDMQSVADNVMVSVASSNTVTPNFWVDPNTGIQYPVAVQVPQYKLASLSDVSGTLVG
                     TRGGSVAGTVPTLLGNVAAFGRDAAQTVTSHSNIQPTFDIYANVEGRDLGRIATDIDA
                     IVAKYRAQLSPGNQIVVEGQIESMQSAFVHMGIGLLVAAVVVYLLMAVNFQSFVDPFV
                     VVLGLPGAMVGIMFMLFATGTSLSVPSLMGAIMSVGVASANSILLVTFAKEQRAFGLS
                     ASDAAVMAGRTRLRPIMMTALAMIVGMIPMALGLGDGGEQNAPLGRAVIGGLMFGTVA
                     TLFLVPWLYSRLRKREGRLMEDFANV"
     gene            2644..3828
                     /locus_tag="VGB93_02975"
     CDS             2644..3828
                     /locus_tag="VGB93_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0145120.1"
                     /translation="MSKPEPAEDFRIAQQHAPKARGGGLRSAGWIALIVMVGGLSFGV
                     GHHISTDSIAEAATDSRLSMVPSVRIVTAHQLKGGIALDLPGSIESFETSTILARASG
                     YISQRFADIGTQVKKGDVLAIIHSPELDQQVSQAQAALLQAKANLDLAHATAARSGAL
                     VGRGWVTGQQYDQDRLTEAARVADVHAAQASLAAITQKRSYLTVIAPFAGVVTARNVE
                     VGDLVSADTTSAQPMFSVARTDKVRVQVYVPQDMAEGLKNGVSASISVPEMPGRTFTA
                     VVARTAQALDATSRTLLAEVDIDNPQNVLTIGTYVRVHFDLVSAKPHVRLDANALIYD
                     ADGLRVAVVKKGHVQLVPVKIVRDYGAEVEIGQGLTGGETVILNPPVALRDGGAVHVA
                     AN"
     gene            complement(3891..4661)
                     /locus_tag="VGB93_02980"
     CDS             complement(3891..4661)
                     /locus_tag="VGB93_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145121.1"
                     /translation="MSKSVAIVTGASQGIGQSTAIRLARDFSALVLVARNRANLEKTA
                     EAVRAAGAETLIIEKDLAEPAAAQAVVDQTLAAFGRIDALLNIAGAVPQIDLFEMTDE
                     QWDAGAALKLHGARRLTIAAWPALKEAKGSVVLMSGNSALFPKAPYAAVGTINAAIVA
                     LAKAFSDRGITDGVQVNSVLPGAVMTGRRRSYLQHWAPLHDLTVEEATANFPKEAGIA
                     RYGEPEEIAELMAFLVSPGARWMTGLTLRMDGGEVKSI"
     gene            complement(4693..5088)
                     /locus_tag="VGB93_02985"
     CDS             complement(4693..5088)
                     /locus_tag="VGB93_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0145122.1"
                     /translation="MTTVPSDVVRALYATLNRGDVPAFLGLLDEEVAWTEQEGFPYYT
                     GPWHGPQAVLKGLVLRLAQDWDNFAATADDFITEGDRVVSFGAYSGIYKATGKHMRAT
                     FAHRWEMRDGKIARFDMFTDTLLIDRALH"
     gene            complement(5132..5998)
                     /locus_tag="VGB93_02990"
     CDS             complement(5132..5998)
                     /locus_tag="VGB93_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0145123.1"
                     /translation="MTAITYQTANVDGFNIFYREAGAPSAPKLLLLHGFPTSSHMFRD
                     LIPLLAGRFHIVAPDLPGFGRSDMPAHDTFAYTFDHIAEIIDRFTEIVGFDRYAVYIF
                     DYGAPTGLRLAVKHPDRITAIISQNGNAYEEGLSEGWNPIQAYWRDASAANREALRAF
                     LKPEATVWQYTHGVSDTTKVSPDGYTLDNFYLARPGADEPQLDLMGDYKSNVALYPTF
                     QEYFRTHKPPLLAVWGKNDPFFLPPGAEAFKRDIPEAIVRFVDTGHFALETHAAKIAT
                     AIRDFLTSTAMR"
     gene            complement(6060..6755)
                     /locus_tag="VGB93_02995"
     CDS             complement(6060..6755)
                     /locus_tag="VGB93_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0145124.1"
                     /translation="MSLHNASVVLAHGAWADGTSWAKIIGPLAARGVQVFAAQLPLTS
                     FKDDVAALDRTLERAAGPVVLVGHAYAGAVIGATRSDKVKALVYVAALAPDEGETVAD
                     VFYRGTPHPQAPKLAPDNHGLIYLPETVFASAFAPNARPEELAILAAVQRPISPACIS
                     VAVERPLWKDRPAWFLVAEQDHMIIAENQRFMAARMNARVRAHSVDHTPLVTAPSLVL
                     DMIDEAIDSVAAD"
     gene            6920..7531
                     /locus_tag="VGB93_03000"
     CDS             6920..7531
                     /locus_tag="VGB93_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY0145125.1"
                     /translation="MGQDQPAIFVADALGLDFLNSIATPVDTPVDWIGDGDGLLDWLK
                     QAQLVPDDALKSIRAQALPGELDNVADQARSLREWFRAFVQKHKGAPLTSRDLSTLGP
                     LNRLLERDEAFRRIESGNSGLSLQTMRKWRSPESLLLPIGEALARFVCSEDFSNVKAC
                     EGPVCTLLFADHTRAHSRRWCSMAMCGNRAKQAAHRHRLKHEH"
     gene            complement(7566..7874)
                     /locus_tag="VGB93_03005"
     CDS             complement(7566..7874)
                     /locus_tag="VGB93_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145126.1"
                     /translation="MMFETAPINNLGALPHIGTPRAKAKRFPRILCNRGDKMPDGKSW
                     SEMSLEEKVEHLYEKTQDVISMRAATEFSDNIVRIVDTLERDLIELRRQTGFRREGSA
                     "
     gene            8072..9259
                     /gene="ssuD"
                     /locus_tag="VGB93_03010"
     CDS             8072..9259
                     /gene="ssuD"
                     /locus_tag="VGB93_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEY0145127.1"
                     /translation="MTELQPPPAAEAADILWFIPTHGDGRYLGTQEGAREVSLAYMRQ
                     IAQAAEDNGYFGVLLPTGRSCEDAWVVASALIPSTQRLRFLVAVRPGLIAPSAAARMA
                     ATLDRISNGRLLINVVTGGDPVELHGDGLFLDHDERYEVTDEFLHIWRGLMAGETVTY
                     KGEHLRIEGGTLLYPPVQKPYPPLYFGGSSAAGHRIAADHVDVYLTWGEPPAEVAKKL
                     AEVRAVAAAKGRQFSYGVRLHVIVRETEAEAWDAANRLIEKVDDETIASAQKIFARFD
                     SVGQQRMASLHGGKRDNLEISPNLWAGVGLVRGGAGTAIVGDPQTVAARIKEYMAVGV
                     DRFILSGYPHLEECYRFAELVFPLLPLRATTGNERRAVRNAGPFGEIIANVVPPSKLA
                     SQS"
     gene            9280..10281
                     /locus_tag="VGB93_03015"
     CDS             9280..10281
                     /locus_tag="VGB93_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0145128.1"
                     /translation="MKPVRSLSAAIIAAMALATPLHAEGSIRIVQQFGTVFLPLDVIR
                     DQRLIEKHGKALGLDITTEWHQLSGGAAVNDALLSGNIDIAGAGIGPFLTVWDRTLDT
                     PSEVKIVGALGAQPNFLLTNKASIKTLKDFTKSDKIALPAAGISVQSRILQIATEQQL
                     GTGHAHDLDDITVTLPHPEAAAGLISGATEITGHVSNSPYQEKELADRKVHKVFSSYD
                     VLGGKVTPTVLYALVKFQKDNPKTFEAFVDALREATAWIATHPKDAAQTFIHVENSKL
                     PPTFIASTLEEPDVEYTVAPLSSKKFADFLYRIGAIKHHPSSWKDYVFEELKRENGS"
     gene            10283..11080
                     /locus_tag="VGB93_03020"
     CDS             10283..11080
                     /locus_tag="VGB93_03020"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145129.1"
                     /translation="MAATAQHLSAVPVLEARGLALDYLGDAGPSRIVENIGFTIESGE
                     RLVLLGPSGCGKSSILKAVAGFLKPAAGVIETRGRKITGPGPDRIVVFQEFDQLLPWK
                     TIRENVAFPLRVARKLGKAEAVERSDAALTAVGLSHAVDFYPHTLSGGMKQRAAIARA
                     LATKPDILLMDEPFAALDALTRRQLQEDLLKLAEELQMTLIFVTHAIDEAVLIGTRLL
                     LLGGQPAHALATFDTSTLGVEDIGLARFDGIVRDVHEVLFGARRSDV"
BASE COUNT         2211 a         3403 c         3316 g         2301 t
ORIGIN      
        1 atggcggccg catccgccag gtgatggtcg atatcgaccc cgccgcgctg gccgccaagg
       61 gcattacgcc gctcgatgtc tccaacgcga tcaatacgca gaacctctca ctgccgatgg
      121 gcgacgtgaa attcggcgac aaggattacg atgtccgcat caatagtctc gccgatgctg
      181 tctcgatcct cgacgacgtg ccgatcaaga aggtcaatgg cgcactgatc cgcatcaagg
      241 atgtggccga ggtccgcgac ggctatgcca tccagcagaa tatcgtgcgc agcgagggcc
      301 gtcgctccgt tcttctctct atcttgaagg ccggtaacgc ctcgacgctg gatgtcgtcg
      361 atcgggtgaa gaacgacgtg ctgccggtca gccgcgccgc cgccccgccc ggcatgaaaa
      421 tcgaggaatt gttcgatcag tcgattttcg tacgctcggc gatcaccggt gtcgccgtcg
      481 aagcgacgat cgcagccctc ctcacctcgg caatgattct gctgtttctc tcaagctggc
      541 gttcgacgct catcgtcgcc gtctcgatcc cgctgtctat cctgtcgtcg attgccattc
      601 tctccgccat tggcgagacg ctgaacatca tgacgcttgg cggtctcgcg ctcgcggtcg
      661 gcattctagt ggacgatgcg acagtgacga tcgaaaatat tcaccggctg cgccagcttg
      721 gcgaaaagct gccggaagcg acgcttcacg gcgccgccgg tatcgcgctg ccaacccttg
      781 tttcgacgct ggcgatctcg gcggtctttg tgtcggtcga atttctcgtc ggcccgtcca
      841 aattcctgtt tacgccgatg gcgctcgcgg tcgtcttcgc gatgcttgcc tcgtacatcc
      901 tgtcgcgaac gctcgtgccg atcatggccg gcttgctgct gtctggcgaa catcatggcg
      961 acgaagcgca tgacgatggc cgccagccct cgattttcac ccgcttttcg cgtggcttcg
     1021 aagctcgttt cgagcggctg cgcgacaatt attcggtgac gttggaaaag ctgatcgaaa
     1081 gcaactggaa ggttccggtg gccgccgtgg ctgtcttagc gatcggcgct gtcctgtaca
     1141 cgcagatcgg gcgcgatttc ttcccggcgg tcgacgccgg cgaattcaag cttcacgttc
     1201 gtgcgccgtc cggcacgcgc atcgagacga ccgaacatat tttcgagaat gtcgagaacg
     1261 tcattcgccg tgtcattccg gattccgagc gcgaactcct cattgacgat attggcgtgc
     1321 cgtttcgcta cgccctgccc ttcgatgacg gcaccaccgt cggcggcaac gacggccaga
     1381 tccttgtctc gttgaaagag ggccacaagc cgaccgcggc ctatatcaaa aagctgcgcg
     1441 acgtgcttgc gcgggaattt ccggatatga tcttttattt ccagcccggt gatatcgtca
     1501 cgcagatttt gaatttcggc ctgcccgcgc cgatcgacgt ccgcgttgtc ggctatgaca
     1561 aggggaacaa tctgcgcatc gtccaggcga tgctagatga gatcaaacat gtcgtgggcg
     1621 tggcggacgc gcatctgcac cagatcgtcg atgcgccgga attgaccgtc gcgatcgacc
     1681 gccagcgtgc ccaggacctt ggcctcgata tgcagagcgt cgccgataac gtcatggttt
     1741 ccgtggcgtc gagcaacaca gtgacgccga acttctgggt cgatccgaat accggcattc
     1801 aatatccggt ggccgtgcag gtgccgcaat ataagctcgc atcgcttagc gatgtcagcg
     1861 gtacgctcgt tggtacacgc ggcggatcag tggcgggcac tgttccgaca cttctgggta
     1921 atgtcgcggc cttcgggcgc gacgcggcgc agaccgtcac gagccattcc aatattcaac
     1981 cgaccttcga catttacgcc aatgtcgaag gtcgcgatct cggccgcatt gcgacggata
     2041 tcgacgccat cgttgccaaa tatcgcgcgc agcttagccc aggcaatcag atcgtcgtcg
     2101 aagggcagat cgagagcatg caatctgcct tcgtccacat gggcattggg ctccttgtcg
     2161 cggcagtcgt cgtctatctg ctgatggcgg tgaatttcca gtcgtttgtc gatccattcg
     2221 tcgtcgtgct tggtctgccg ggcgcgatgg tcggcatcat gttcatgctg ttcgcgaccg
     2281 gcaccagtct gagcgtgcca agcttgatgg gtgcgatcat gagcgtcggc gtcgcatcgg
     2341 ccaattccat cctgcttgtc acctttgcca aagagcagcg cgctttcggg ctttcggcca
     2401 gcgacgcggc cgtcatggct ggccgtacgc ggctgcggcc gatcatgatg accgcgctcg
     2461 ccatgatcgt cggcatgatt ccgatggcgc tcggtctcgg tgacggcggc gaacagaacg
     2521 cgccgctcgg ccgcgccgtg atcggcggcc tgatgttcgg cacggttgcg acgctctttc
     2581 ttgttccctg gctttatagc cgcctgcgca agcgcgaagg ccggctgatg gaggattttg
     2641 ccaatgtcta agcccgagcc cgccgaagat ttcagaatcg cgcaacaaca cgcgccgaag
     2701 gcacgcggcg gcggattgcg ttcggccggc tggatcgccc tgatcgtcat ggtcggcggt
     2761 ctcagctttg gtgtggggca ccacatctcg acggactcga tcgctgaagc cgcgacggat
     2821 tcacgtctct cgatggttcc gagcgtgcga attgtgacgg cgcatcaatt gaagggcggc
     2881 atcgcgctcg acttgccggg gagcattgaa tctttcgaga cgtcgacgat tctcgcacgc
     2941 gcctcaggct atatttcgca acgcttcgcc gatatcggga cgcaggtaaa gaagggcgat
     3001 gtgctcgcga tcatccactc acctgagctt gatcagcaag tgagccaggc acaggcggcg
     3061 ttactgcaag ccaaggccaa cctcgatctc gcgcatgcga cggcggcgcg ctccggcgct
     3121 ctcgtcggtc gcggatgggt cacaggccag caatatgacc aggatcgttt gacggaagcg
     3181 gcccgcgttg ccgatgtcca cgccgcgcaa gcctcgctcg cggcgatcac tcaaaagcgc
     3241 agctatctga cggtcatcgc gccgttcgcg ggcgtcgtca ccgcccgcaa tgtcgaagtc
     3301 ggtgatcttg tcagtgccga tacgacctcg gcacagccga tgttcagcgt cgcgcgaacc
     3361 gacaaggtgc gcgtgcaagt ttatgtcccg caggacatgg ccgagggcct gaaaaacggc
     3421 gtctccgcgt cgatctcggt gcccgagatg ccgggccgca catttaccgc tgtcgtcgcg
     3481 cgaaccgcgc aggcgctgga cgcgacatcg cgaaccctgc tcgccgaagt cgatattgac
     3541 aacccgcaga atgtcctgac gatcggcact tatgtgcggg ttcacttcga tcttgtctcg
     3601 gcgaagccgc atgttcgtct cgacgcgaat gcgttgatct atgatgccga tggcctgcgt
     3661 gtcgccgtgg tcaagaaagg tcacgtccag cttgttccgg tgaagatcgt tcgcgactac
     3721 ggcgccgagg tcgaaatcgg ccagggcctg actggcggcg aaaccgtgat cctcaaccca
     3781 ccggtggcgc tccgggacgg cggcgcggtt catgtcgcgg cgaattgaga aatagtccgg
     3841 ccgcataatg cattcagacg caggaaacta aagcttgcgc cttggcgcgt tcagatcgat
     3901 ttgacttctc cgccatccat gcgcagcgtc aagccggtca tccagcgcgc gccgggggat
     3961 acaagaaacg ccatcagctc ggcgatctcc tcgggttcgc catatcgcgc gattccggcc
     4021 tccttcggaa agttggccgt cgcctcctcg accgtcagat catgcagcgg cgcccaatgc
     4081 tggagatacg aacgccggcg acccgtcatc acggcacccg gcaagacact attgacctgc
     4141 acgccgtcgg tgatgccgcg atccgaaaac gccttcgcta acgcgacaat cgcggcgttg
     4201 atcgttccca ccgcagcata aggcgccttc gggaacagag ccgaatttcc cgacatcaac
     4261 acgacggaac ctttcgcctc cttcaaagcc ggccaagcgg cgatcgtcag ccgccgggcg
     4321 ccatggagtt tcagcgcggc gccagcgtcc cattgttcgt cggtcatctc gaagagatcg
     4381 atctgcggta ccgcgccggc gatattgagg agcgcgtcga tacggccgaa agcggcgagc
     4441 gtctgatcga ccacggcctg cgcggcggcg ggttcggcga gatccttttc gatgatcagc
     4501 gtttcggcgc cagctgcccg caccgcttct gccgtctttt ccagattggc gcggtttcgt
     4561 gcgacgagga ccaatgccga gaaatcgcgc gcaagccgga tggccgtgga ttgaccgatg
     4621 ccttggctgg cgccggtgac aatggcaact gactttgaca tggcaagtct ccttggaagc
     4681 tggctgatgc cgtcaatgca gcgcccgatc gatcaatagc gtgtcggtga acatgtcgaa
     4741 gcgggcgatc ttgccgtcgc gcatctccca acgatgggca aaggtcgccc gcatatgctt
     4801 gccagtcgcc ttgtagatgc cagaataggc accgaaggag acgacccggt cgccttcggt
     4861 aatgaaatcg tcagccgtcg cggcgaaatt gtcccaatcc tgcgccaatc gcaggaccag
     4921 gcctttgagc acggcctgcg gcccatgcca gggccccgtg tagtatggga agccctcctg
     4981 ttcggtccag gctacctcct cgtccagcag cccgagaaag gccgggacat cgccccggtt
     5041 gagagtggca tagagcgcac gaaccacatc tgagggaacg gtcgtcattt cagaatctcc
     5101 taaagtgagt ttcggcgaat gaatgcggca atcagcgcat cgcggtcgaa gtgaggaaat
     5161 cgcggatcgc cgtggcaatc ttcgccgcat gcgtctcaag tgcaaaatgg ccggtatcga
     5221 caaagcgcac gattgcctca gggatgtcgc gtttgaacgc ctcggcgccc ggcggcagga
     5281 agaacggatc gttcttgccc cacacagcca gcaacggcgg cttatgggtg cggaaatatt
     5341 cctgaaaggt ggggtagagc gcgacgttgc ttttgtaatc gcccatgaga tcgagttgtg
     5401 gttcgtcggc gccaggccgc gcgagataga aattgtcgag cgtatagccg tccggcgaaa
     5461 ccttcgtcgt gtccgagacc ccgtgcgtat attgccagac tgtcgcctca ggcttgagga
     5521 aagcgcgtag cgcctcgcgg ttagcagccg aagcgtcgcg ccaatacgcc tggatcggat
     5581 tccagccttc gctcaacccc tcctcgtaag cattgccgtt ttgcgagatg attgccgtga
     5641 tccggtcggg atgtttcacc gcgaggcgaa gtccggtggg cgcgccgtag tcgaaaatat
     5701 agacggcgta acgatcgaaa ccgacgattt cggtgaagcg atcgatgatt tcggcaatat
     5761 ggtcgaacgt ataagcgaat gtgtcgtgcg ccggcatatc cgaccggcca aatccgggca
     5821 ggtccggtgc gacaatatga aaacgaccgg caagaagcgg aatgagatcg cgaaacatat
     5881 ggctcgaagt cggaaagccg tgaagcaaga gaagcttcgg tgcgctcggc gcacctgcct
     5941 cgcgatagaa gatgttgaag ccatcgacgt tggcggtttg ataggtgatc gcggtcatga
     6001 gcttttcctc actgaagtaa cttggtaata gctacaacga cggttacggt cttctggctc
     6061 taatctgcgg cgacgctgtc gattgcctcg tcgatcatgt cgaggacaag agaaggtgcc
     6121 gtgacaagcg gtgtatgatc gacggaatgg gcacgcacgc gggcattcat ccgcgcggcc
     6181 atgaaacgct gattttccgc gatgatcata tggtcctgct ctgccacaag gaaccacgcc
     6241 ggacggtctt tccagagcgg ccgctcgacg gcgacgctga tacatgccgg cgaaatcggc
     6301 cgctggaccg ccgccaatat agccaattcc tccggccgcg cattcggcgc aaaagcagag
     6361 gcgaagactg tttcaggcag gtagatcaag ccgtgattgt caggcgcgag ctttggtgcc
     6421 tgaggatgcg gtgtgccgcg gtagaaaacg tcggcgaccg tctcgccttc gtcaggcgct
     6481 agtgccgcca cataaacgag agccttcact ttgtcgctgc gcgtcgcccc aatgacggct
     6541 ccggcgtagg cgtgaccgac gaggacgacc ggccccgcgg cgcgttcgag cgtccgatca
     6601 agagcagcaa catcgtcttt gaaagatgtc aaaggcaatt gcgctgcaaa cacttgaacg
     6661 ccgcgcgcgg ctagcggccc gatgatcttt gcccagctcg tcccgtcggc ccaggcacca
     6721 tgtgccaaga caacactcgc gttatgcaat gacataattg cctccctctg accgagctaa
     6781 aactaacctc tttaaattca ttatgacggt tactatagca atagataact tgtcaatagc
     6841 cgacagacag gttattttag gcgtatattc acggatgggt gccgcgcgga caccgcgatt
     6901 ggctccggag gtaagcttga tgggccaaga ccaacccgca atttttgtcg ccgacgcact
     6961 cgggctggat ttcctgaatt cgatcgcgac gccggtcgat acgcccgtcg actggatcgg
     7021 cgatggcgat ggcctgctgg attggctgaa gcaggcgcag cttgtgcccg acgacgcact
     7081 caagagcatt cgggcacaag cgcttcccgg cgaactcgac aatgtcgcgg atcaggcgcg
     7141 aagccttcgc gaatggttca gagcgtttgt acaaaagcac aaaggcgctc ctctcacatc
     7201 gcgggattta agcactctgg ggccattgaa tcgtttgctc gaacgcgatg aagcattcag
     7261 gcgcatcgag agcgggaatt cgggcctctc acttcaaacc atgcgcaagt ggcgctcgcc
     7321 agagtcactt ctgctgccga ttggcgaggc tctagcgcga tttgtgtgct cggaagattt
     7381 ttcgaacgtg aaagcttgcg aaggccccgt ctgcaccctg ctttttgccg atcacacgcg
     7441 cgcacattcg cggcgctggt gcagcatggc gatgtgcgga aatcgcgcaa aacaggccgc
     7501 gcatcggcat cggctcaagc acgaacatta gttgtgaggc gttgtgaggc gatgcacgcc
     7561 ccgcctcacg ccgaaccttc gcggcgaaag ccggtttgcc gccgcagttc gatcaaatcc
     7621 ctctccaggg tatcgacgat cctgacgata ttgtcggaaa actcggtcgc cgctcgcatt
     7681 gaaatcacgt cttgcgtttt ctcgtaaaga tgctcgacct tttcctccaa cgacatctcc
     7741 gaccaagatt tcccatccgg cattttgtcc ccccgattgc ataaaatacg tgggaatcgc
     7801 tttgccttag cccgaggcgt tccgatgtga ggtaacgcac ctaaattatt gatcggcgcg
     7861 gtttcgaaca tcatcaattc gtgaaagggc aacgagcgtc gacgccgccc tcaatacaat
     7921 ttctcgggcc gcgttcgatt tcgttcgaac cgcgcgtcca tgcggatgca aacggtccaa
     7981 cccgcgctta gatatatagt ctaccgttta tattgactta tgcagcgcgc ctatggtgaa
     8041 ttcgcccact cgtctctcac cggatactga aatgactgag cttcaacccc cgccagccgc
     8101 ggaagcggcg gatattcttt ggtttattcc gacgcatggc gacggccgct atctcggaac
     8161 ccaggaaggc gcgcgcgaag tttcgctcgc ctatatgcgc cagatcgcgc aggcagcgga
     8221 ggacaacggc tattttggtg tcctgctgcc aacggggcgt tcatgcgagg atgcctgggt
     8281 cgtcgcatcg gcgctgatcc cctccacgca aaggttgcgc ttcctcgtgg ctgtcaggcc
     8341 gggcctcatc gcgccatcgg cggcggcgcg catggcggcg acgctcgatc gcatctccaa
     8401 cggccgtttg ctgatcaatg tcgtcaccgg cggcgatccc gtcgagcttc atggcgacgg
     8461 cttgtttctc gatcacgacg agcgctacga ggtgaccgac gaatttctgc acatctggcg
     8521 cggcctgatg gcaggcgaga ccgtcaccta caagggcgag catctcagaa tcgaaggcgg
     8581 cacgcttctc tacccgccgg tgcagaagcc ctatccaccg ctctatttcg gcggttcctc
     8641 cgccgctggt catcgcatcg cggcggacca tgtcgacgtc tatctcacct ggggcgaacc
     8701 accggccgag gtcgccaaga aattggcaga agtgcgcgcg gtcgccgcgg ccaagggacg
     8761 ccagttctcc tacggggttc gtctccatgt catcgtccgt gaaacggaag ccgaagcttg
     8821 ggatgcggca aacagattaa tcgaaaaggt cgacgacgag accatcgcgt cggcgcagaa
     8881 aatcttcgcg cgtttcgatt ccgtcggcca gcaacgcatg gcaagtttgc acggcgggaa
     8941 acgcgacaat cttgagatca gccccaatct ctgggccggc gtcggcctgg tccgcggcgg
     9001 cgctggcacg gcgatcgtcg gcgacccgca gacggtggcg gcgcggatca aggaatatat
     9061 ggcggtcggc gtcgatcgct tcatcctctc cggttatccg catctggagg aatgctaccg
     9121 cttcgcggaa ctggtgtttc ccctgctgcc gctgcgcgcg acaaccggca acgaacgcag
     9181 ggccgtgcgc aacgccgggc ccttcggtga aatcattgcc aatgtcgttc caccttcaaa
     9241 gctcgcttca cagtcctgat ctcaaaccaa gaggtgcgcg tgaagcccgt ccgttcgctc
     9301 agcgccgcaa tcatcgccgc tatggcgctt gcgacgcctc tccatgcgga gggatcgatc
     9361 cgcatcgtgc agcaattcgg cactgtgttc ctgccgctcg atgtcatccg cgatcagcgc
     9421 ctcattgaaa agcacggcaa ggccctcggt ctcgacatca cgaccgaatg gcatcagctc
     9481 tcgggcgggg ctgcggtcaa tgatgcgctt ctgtcaggaa atatcgacat cgcgggcgcc
     9541 ggtatcggac cttttttgac cgtctgggat cggacgctcg atacgccgag cgaggtcaag
     9601 attgtcggcg ctctcggcgc acagccaaat tttcttctga ccaacaaagc cagcatcaag
     9661 acgctcaaag acttcacaaa gtccgacaag attgccctcc ccgccgccgg gatttccgtt
     9721 caatcgcgaa tcttgcaaat cgcgaccgag cagcagttag gcacgggtca cgcgcacgac
     9781 cttgacgaca tcaccgtcac gcttccgcat ccggaggcgg ccgccgggct gatatccggg
     9841 gcgacggaaa tcaccggcca tgtctcaaac tcgccgtatc aggagaaaga gctggcggat
     9901 cgaaaggtcc ataaagtctt ttcatcttac gacgttctgg gtggcaaggt gacaccgacc
     9961 gtactttacg ccctcgtcaa attccaaaaa gacaatccga agacgtttga agcgttcgtc
    10021 gatgcgttgc gcgaggcgac agcctggatc gccacgcatc caaaggacgc cgcacaaacc
    10081 ttcatccacg tcgagaactc gaagcttccg ccgaccttca tcgccagcac gctcgaagag
    10141 ccggatgtcg aatatacagt cgcaccattg tcctcgaaaa aattcgccga ttttctctac
    10201 cggatcggcg cgatcaaaca tcatccgtcg agctggaaag actacgtctt cgaagagctg
    10261 aagcgcgaaa acggaagcta gaatggccgc gaccgcccag cacttatcgg ccgtgccagt
    10321 tttggaagcg cgcggcctcg cgctcgatta tttgggtgac gccggcccat cgcgcatcgt
    10381 cgaaaatatc gggttcacga tcgaatccgg cgaacggctc gtgctgctcg ggccctccgg
    10441 ctgcggcaaa tcttcgattt tgaaagccgt cgccggcttc ctgaaacccg cggcgggcgt
    10501 gattgagaca cgcggccgca agatcaccgg cccggggccc gatcgtatcg tcgttttcca
    10561 ggaattcgac caattgctgc cctggaaaac gatccgggaa aatgtcgctt ttccgctgcg
    10621 tgtcgcgcgc aaactcggca aagccgaagc cgtcgaacgc tccgacgcgg cgctcaccgc
    10681 cgtggggctg tcacacgccg tggatttcta tccgcatacg ctttccggcg gcatgaaaca
    10741 gcgtgcggcg atcgcgcggg cgctggcgac caaacctgac atcctgctga tggacgaacc
    10801 gttcgcggcg cttgatgctc tgacgcgtcg ccagcttcag gaagatctgt tgaagttggc
    10861 agaagaactt cagatgactt tgatcttcgt cacgcatgcg atcgacgagg cggtcttgat
    10921 cggcacgcgg cttctgcttt tgggcggcca gcctgcgcac gcccttgcga cgttcgacac
    10981 atccacattg ggagttgagg acatcggctt ggcgcgtttt gacggcattg tccgggatgt
    11041 acacgaagtt ttatttggcg cgagacgatc agatgtctga actcaatctc gcgcaccgcg
    11101 aaccgtcgct tgcgagcgcc ggaaagccgg gggcctcggc ttttgtgctt cgtagcttaa
    11161 attcgctgcg ccgctccttt atcgcgcgcc gactatcggt tctcgttcta ttggcaattg
    11221 cctggcagat c
//
LOCUS       DASWOG010000149         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_205020,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000149 DASWOG010000000
VERSION     DASWOG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205020"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGB93_03025"
     CDS             <1..546
                     /locus_tag="VGB93_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0145130.1"
                     /translation="LPAFASLAGDITWHIATLSKCVSPALRIAYVIAPGNNQAQRLAG
                     VLRATTLMAPPLMAALASRWIGDGTLDEISGAIRVENAARQEMAAAILKDVSFAADPN
                     GHHLWLNMPSHWRANDFTEHADRSGVAIVSASAFAIASGPPEAVRISLGVAPDRGALE
                     DALILLAELLSQPSLMARAVV"
     gene            614..>1779
                     /locus_tag="VGB93_03030"
     CDS             614..>1779
                     /locus_tag="VGB93_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0145131.1"
                     /translation="MMFGLTALELARIQFGFTVSFHIIFPAITIGLASFLVVLEALYL
                     WKKDETYRDLYHFWSQIFAVNFGMGVVSGLVMAYQFGTNWSYFSAFAGSVTGALLTYE
                     VMTAFFLEAGFLGVMLFGWNKVGRGLHFLSTIMVTLGTHISAGWILASNSWMQTPRGF
                     TVVGGRVIPLDWWAIIFNPSFPYRFVHMTIAAYLAVALFVGATGAWHLLRGRDTKAVR
                     TMFSMAMWLILCVAPIQIIAGDQQGLNTLKYQPAKIAAIEGHWINVPHESVPLVLFGW
                     PDMKDGKTLDEVDVPHLGSLVLTHSWNGQVPGLDDFPPQDRPDSTVIFWTFRLMVGLG
                     FLMLGLGLWSGWLRWRHEFFVTRPFLWLALVMGPAGLIAILSGWITTEMGRQPWV"
BASE COUNT          294 a          505 c          548 g          432 t
ORIGIN      
        1 ttgccggcgt tcgcaagtct ggcaggggac atcacctggc acatcgcgac gctctccaaa
       61 tgtgtcagcc ctgccttgcg gattgcctat gtcattgcgc ccggcaacaa tcaggcgcag
      121 cggctggcgg gtgtgttacg cgcgacgacg ctcatggcac cgccgttgat ggcggcttta
      181 gcgtcacgat ggattggcga tggaacgctt gatgaaattt ccggtgccat tcgcgtggag
      241 aatgcagcgc ggcaagagat ggccgctgcg attctgaaag acgtgagttt tgccgcagat
      301 ccaaatggcc atcatctgtg gttaaacatg ccgtcacatt ggcgcgccaa tgattttacc
      361 gaacatgcgg atcgctccgg tgtggcgatc gtgtcggcgt cggcattcgc gattgcatct
      421 ggcccgccgg aggcggtcag gatatcgttg ggcgtggcgc cggatcgtgg cgcgctcgaa
      481 gatgcgttga ttttgctggc ggagcttctc tcacagcctt cgcttatggc acgcgctgtc
      541 gtttaactgg aagatgcagc acatctcgtg ctcataatgt gtagcccgaa gcagtgacgg
      601 taaataggga cagatgatgt tcggtctgac ggcattagag ctcgcgcgga tacaattcgg
      661 gttcaccgtc tcgttccaca tcatctttcc ggcgatcacg attggcctcg cgagctttct
      721 cgtcgtcctc gaagctttgt atttgtggaa gaaggacgag acctatcgcg acctgtatca
      781 tttctggtcg cagatctttg ctgtcaattt cggcatgggc gtcgtctcgg gcctcgtcat
      841 ggcctaccag ttcggcacca actggagcta tttttcggct ttcgccggca gcgtcacggg
      901 cgcgcttctt acttatgagg tgatgacagc gttcttcctc gaggccgggt ttctcggcgt
      961 catgttgttc ggctggaaca aggtcggccg cggcctgcat ttcctttcaa ccatcatggt
     1021 gacgctcggc acgcatattt cggcaggatg gattctcgcg tcgaacagtt ggatgcagac
     1081 accgcgcgga ttcaccgtcg tcggcggccg cgtgatcccg cttgactggt gggcgatcat
     1141 cttcaatccg tcgttcccct accgcttcgt acatatgacg attgcggcct atctcgcagt
     1201 cgcacttttc gtcggcgcga cgggcgcctg gcatctgctg cgcgggcgcg acacgaaggc
     1261 cgtgcgcacg atgttctcga tggcgatgtg gctgatcctt tgcgttgcgc cgatccagat
     1321 cattgccggc gatcagcagg gcctcaacac gctcaaatat cagccggcga agatcgccgc
     1381 gatcgaaggc cattggatca acgtgccgca cgaatccgtg ccgcttgtcc tcttcggctg
     1441 gccggacatg aaagacggca agacgctgga cgaggtcgac gtgccgcatc tcggcagcct
     1501 ggttctgacg cactcgtgga atggacaggt gcccggcctt gacgattttc cgccgcagga
     1561 ccggccggat tctaccgtga tcttctggac gttccgtttg atggtcgggc ttggctttct
     1621 gatgctgggc cttggtctct ggagcggctg gctgcgctgg cgtcatgagt tcttcgtcac
     1681 gcgtcctttc ctatggcttg cgctggttat gggcccggcc gggctcatcg caattctgtc
     1741 cggttggatc accacggaga tgggccgcca gccttgggt
//
LOCUS       DASWOG010000150         8800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_95286,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000150 DASWOG010000000
VERSION     DASWOG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95286"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VGB93_03035"
     CDS             <1..339
                     /locus_tag="VGB93_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY0145132.1"
                     /translation="EGYGNFGFTFGGMSHRGSILALPSGIYAWDVLGPEDLGVGALAQ
                     VFAEPKGEIEHLLIGTGVELRPLAKELRAALNRDGIRVEPMATGAAARTYSILLGENR
                     RVAAALIAVA"
     gene            346..1158
                     /locus_tag="VGB93_03040"
     CDS             346..1158
                     /locus_tag="VGB93_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY0145133.1"
                     /translation="MDHAADYTYCEALLRRNDPDRWLASLFLPVALRSHVVALYAFSD
                     EIARVRAHVSEPLLGEIRFQYWRDVLTGERGGDAPVAAALVDTVERFRLPREKLISLI
                     DARLFDLYDAPMPSVTALESYAAATAGSLFQLAAFILDPAADVAAASRHAGIAYAVTG
                     LLRGLPWQVAAGQTYVPRELYAGSDLKTDRPALDAALAKLRVLVRQNLAALATEPATK
                     SGQAAPAFLPAALCELYLPQMEKPGYDPLATLVEVPQWRRQWRLWRAARKIV"
     gene            1327..4368
                     /gene="ileS"
                     /locus_tag="VGB93_03045"
     CDS             1327..4368
                     /gene="ileS"
                     /locus_tag="VGB93_03045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY0145134.1"
                     /translation="MNEQTRAAKKAEGPDYSKSLYLPQTDFPMRAGLPQKEPELLARW
                     REIELYRRLREAGQGRPKFVLHDGPPYANGNIHIGHALNKILKDLVTRSQQMTGKDSN
                     YVPGWDCHGLPIEWKIEEEYRAKGKNKDEVPIVEFRRQCRAFAAHWVDVQREEFKRLG
                     VIGDWDHPYETMTFSAEAQIAREIMKFAENGTLYRGSKPVMWSVVEKTALAEAEVEYE
                     DHVSDTVWVAFPVTSVGNVRVVGEDISVAQKRALLNEAKIVIWTTTPWTIPANRAISY
                     SSKIAYGIYRVSAAPENNWAKAGATFLLADALADSVLKAAKVGAYEKVAPVYAEELVG
                     FTAAHPLARTLLGYDFDVPLFDGEHVTDDTGTGFVHTAPGHGRDDFEIWMAHGRELAA
                     RGIETRLPYTVDADGFLTKDAPGFTGKRVLDDRGNKGDANEAVIKALVEAGALVARGR
                     LKHQYPHSWRSKKPLIFRNTPQWFIALDKPIEGLGGKTQGNNGGPSLREIALEEIART
                     QWFPAQGENRITGMVAGRPDWVVSRQRAWGVPIAIFVRKETNDILVNAQVNARIADAF
                     EKEGADAWFAPDAGARFLAPDYDPKDFDKIDDVLDVWFDSGSTHAFTLEGAKDFPGLA
                     GIKRTRDGGRDSVMYLEGSDQHRGWFQSSLLESCGTRGRAPFDAVLTHGFVLDEKGQK
                     MAKSVGNVVAPQTVIAQSGADILRFWVAASDYSDDLRIGPEILKTFVETYRKLRNTIR
                     WMLGSLAHYDAAAKQVIVADMPELERLMLHRLAELDSDIREAYARFDYKRVVARLSVF
                     LNTDLSAFYFDIRKDTLYCDPPSSLKRHASLAAIEQIFRAVTLWLAPILAFTCEEAWL
                     SRYPDATSVHLEAFPQIPAEWRDDALAAKWEKIRRVRSVVTGALEIERGQKRIGSSLE
                     AAPAVHIADPDLRAAVTATDFAEVCITSDIAVEAGEGPAEAFRVADIPGVAVVPARAP
                     GRKCARSWKISPGVGSDAEFPDVTPRDAQALRELVALGLWP"
     gene            4365..4907
                     /gene="lspA"
                     /locus_tag="VGB93_03050"
     CDS             4365..4907
                     /gene="lspA"
                     /locus_tag="VGB93_03050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY0145135.1"
                     /translation="MSAQADGPLPVSKATSSPVRVRVIGLLTALATFFLDQANKLWLI
                     FDYGIATHQPIHLAPFFDVIFLKNPGISYSLLTASSAFGRWALLAIALVATACLILWL
                     WRTRLMLTGFGLGLIIGGAVGNAADRLAYGYVADFYHFHVGSFSWYVFNLADVAICIG
                     VGLLVLEGLTEKPRANDAVE"
     gene            4981..5709
                     /locus_tag="VGB93_03055"
     CDS             4981..5709
                     /locus_tag="VGB93_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145136.1"
                     /translation="MPFDSRRTRNLVAATSGVALAALLAASPARADGDMWNSMLGWVG
                     MGHKSAPSTDGPDDGAIDYTPKPALVVPPKMDLPPPQASTSTPADWPHDPDATARAKA
                     EADSRRPAPSSDSTSDNSAGDFVADADKAKPDNGESEACSNTAGKPICFYGPSGLMNF
                     AKGWNMGGMFSTHAEDTSKLRIGSEPPREYLIQPPPGYQMPKVKDEDDSDADSGADAS
                     KYDSVDVGATRRHHHSSTDSSTAN"
     gene            complement(5756..6781)
                     /locus_tag="VGB93_03060"
     CDS             complement(5756..6781)
                     /locus_tag="VGB93_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145137.1"
                     /translation="MQGLSWLSGNKALAYGAAAVALIVAALLVLLVFRLAFGRRLRMA
                     GARGRQLRLGIVDAFDLDRQRQLVIVRRDNVEHLIMIGGPNDMLIESQIVRAEAREPR
                     QRDKDRDKPREKDFEAMQLPAAQPLKPTSVEPHLPLPPFVPEAPAEDPVTARQIPPVT
                     PTIVTAAPAPVQPIARAPLATPVGPRRTPPPVRPDVKPAPAPFPPTPAVPSPVAIEPA
                     LPSAEAMLPAKPTTAPAQEGQAPSQATTQGFMRWRPNQNPAQPQSRPAGPSFTQPIRP
                     RDPSPTTPVPPVVENKPAPEFELAPPVSAEPKEHVSVASAPGAEDLMVSLEEEMAKLL
                     GRTPDKP"
     gene            7141..>8800
                     /locus_tag="VGB93_03065"
     CDS             7141..>8800
                     /locus_tag="VGB93_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0145138.1"
                     /translation="MTDQPTTAAFDRTERAGSPGLVLLFAACLVGPIAAFSVLPKDQA
                     GTMVIGLLTILAVIGIFALFGYAVGFFQFSGQAARNDLTRLICDSEPEGAIVTDNAGK
                     TLYANEAYMRLSGARDVSQLKAVERLLSGSSDVSESIYRLAQAAREGKRNNEELRLAP
                     PLTGDSEVGWYRVRVRPLNFSGAKRATLWSVADVTRERERHENVFQELQHAIDFLDHA
                     PAGFFSCDASGAVSYMNATLAGWLDYDLAQVGSGGLELNDFVAGGGAVLLASVAGAPG
                     DVVTDQFDMELKRRNGQSLPVRMLHRVAFGQDGLPGASRTLVFNRAPGEEPAEDLRAA
                     EVRFARVFLAAPMAMAIVDRNGRIKRSNTAFAKLMPEALRAADSAQRSIRGGISDTDH
                     PALDSAIAAAAGSKTDIPPVDVQLSGNRSARLLFSASDEADGPGATIYALDTTELRTL
                     KENFAQSQKMQAIGQLAGGVAHDFNNVLTAIIGYSDLLLANHRPTDPSFQDIMQIKQN
                     ANRAAGLVRQLLAFSRRQTLRPEVLQLGDVLSELQLMMRRLVGEK"
BASE COUNT         1556 a         2664 c         2830 g         1750 t
ORIGIN      
        1 gagggctatg gcaatttcgg cttcaccttt ggcggcatgt cgcatcgcgg ctcgatcctg
       61 gctctcccgt ccggcatcta tgcctgggac gtgttagggc cggaagatct cggcgtcggc
      121 gcgttggcgc aggtcttcgc cgaaccgaaa ggtgaaatcg aacatctgct gatcggcacc
      181 ggcgtggaac tgcggccact ggccaaggaa ctccgtgctg cgctcaatcg cgacggaatt
      241 cgcgtcgagc cgatggcgac gggggcggcg gcgcggacct attcgatcct gctcggtgaa
      301 aaccgtcgtg tcgcggcggc gctgatcgcc gtggcttgag cggcgatgga tcacgcggcc
      361 gattacacat attgcgaggc actccttcgg cgcaacgatc ccgaccgctg gctggcaagc
      421 ctgtttctgc cggtggcgtt gcgctcgcat gtcgtcgcgc tctatgcctt tagcgacgag
      481 atcgcccgcg tccgcgcgca tgtgagcgag cctttgctgg gtgaaatccg cttccaatat
      541 tggcgcgatg tgctgacggg cgagcgcggc ggggatgcgc cggtcgcggc ggcgctggtc
      601 gatacggtcg agcgcttccg gctgccacgc gaaaaactca tcagcctcat cgatgcgcgc
      661 ctgttcgatc tctacgacgc gccgatgccg agcgtcacgg ccctcgaatc ctacgctgcg
      721 gcgacggcgg ggtcgttatt tcaactcgcg gcgttcatcc tcgatcccgc ggccgacgtc
      781 gcggcggcat cgcggcacgc ggggatcgcc tatgccgtta ctggcctttt gcgcgggctg
      841 ccctggcaag ttgctgccgg gcagacctat gtgccgcgcg agctttacgc tggctcggac
      901 ttgaagaccg atcgcccggc gctcgatgcc gccttggcaa agctgcgggt gctggtgcgt
      961 cagaatctgg ccgctttggc taccgagcct gccaccaaat ccgggcaggc ggcgccggcg
     1021 tttctccccg ccgcgctgtg cgagctttac ctaccgcaga tggaaaagcc gggctacgat
     1081 ccgctcgcga ccctcgtcga ggttccgcaa tggcggcgtc agtggcgact ctggcgcgcg
     1141 gcgcggaaaa tcgtctagct ggcgcttgcc ggtttgcagg atgccgggaa tggtctagaa
     1201 ggccgaatgt tcagaaaagc ttcatccgcg gcccgaatta tcagcccggc cggcgcctaa
     1261 gccagcgcgt cgccgggcgt tgcgtagtct tccgcggctc tgccgcacga tcaggccttt
     1321 gcacagatga atgaacagac acgggcggcc aagaaggccg aaggcccgga ttattcaaaa
     1381 agcctctatc tgccgcagac ggattttccg atgcgcgccg gtctgccgca gaaggagccg
     1441 gaattgctcg cgcgttggcg cgagatcgag ctttaccggc gcctgcgtga agccgggcag
     1501 ggacggccga aattcgtcct ccacgacggg ccgccttacg ccaatggcaa catccatatc
     1561 ggtcatgcgc tgaataaaat cctgaaagac ctcgtcacgc gctcgcagca gatgacgggc
     1621 aaggattcga attacgttcc aggctgggac tgccacggcc tgccgatcga atggaagatc
     1681 gaggaagaat atcgcgctaa gggcaagaac aaggacgagg tgccgatcgt tgagtttcgt
     1741 cgccaatgcc gcgccttcgc cgcgcattgg gtcgatgtgc agcgcgagga attcaaacga
     1801 ctcggtgtca tcggcgactg ggatcatccc tacgagacga tgaccttctc ggccgaggcg
     1861 cagatcgccc gcgagatcat gaaattcgcc gaaaatggca cgctctatcg cggctcaaag
     1921 ccggtgatgt ggagcgtcgt cgaaaagacc gcgctggccg aagcggaagt cgaatacgaa
     1981 gaccatgtca gcgacacggt ctgggtggcg tttccggtca cgtcggtcgg caatgttcgc
     2041 gttgtcggcg aggacatcag cgttgcgcaa aaacgcgctc tgctcaacga ggcgaagatt
     2101 gtcatctgga cgacgacgcc ttggaccatc cccgcgaacc gcgcgatcag ctattcttcg
     2161 aagatcgcgt atgggattta tcgcgtcagc gctgcgccgg agaacaattg ggcgaaggcc
     2221 ggggcgacct ttctcctcgc cgatgcgctc gccgacagtg tgctgaaagc cgcgaaggtc
     2281 ggggcttatg agaaagtcgc gcccgtctac gcggaggagc tagttgggtt taccgcggcg
     2341 catccgctcg cgcgtacatt gctaggttat gactttgacg tgccgctttt cgacggcgag
     2401 catgtcactg atgacactgg cacgggcttt gtccatactg cgcccggcca cggccgcgat
     2461 gacttcgaaa tctggatggc gcacggccgt gaattggcgg ctcgcggcat cgaaacgcgg
     2521 cttccttaca cggtcgatgc cgatggtttc ctgacgaaag acgcgccggg cttcaccggc
     2581 aaacgcgtcc tcgatgacag aggcaataag ggcgacgcca acgaggccgt catcaaggct
     2641 ttggtcgagg ccggtgcgct cgtcgcccgc gggcgtctga agcatcagta cccacattcc
     2701 tggcgctcga agaagccact catctttcgc aatacgccgc aatggttcat tgcgctggat
     2761 aagcccatcg aaggtctcgg tggaaagacg cagggcaaca atggcggccc gtcgcttcgc
     2821 gagatcgcgc ttgaggaaat cgcgcgtacg caatggtttc cggcgcaggg cgagaaccgc
     2881 atcacaggca tggtcgcggg tcgcccggat tgggtcgtct cgcgtcagcg cgcctggggc
     2941 gtgccgatcg cgattttcgt gcgcaaggag acgaacgaca ttctcgttaa tgcccaggtg
     3001 aatgcgcgga tcgccgacgc cttcgagaaa gagggcgccg acgcctggtt tgcgcccgat
     3061 gctggcgcac gttttcttgc gcccgactac gatccgaaag acttcgacaa gatcgacgat
     3121 gttctcgatg tttggttcga ttccggttcg acccacgcgt tcacattgga aggtgcgaag
     3181 gattttcctg gcctcgccgg tattaagcgc acgcgcgatg gtgggcgtga ttccgtgatg
     3241 tatcttgaag gttccgatca gcatcgtggc tggttccaat catcgctgct cgaaagctgc
     3301 ggcacgcgcg ggcgcgcgcc cttcgatgcg gtgctgacgc acggattcgt tctcgacgag
     3361 aagggtcaga aaatggccaa gtccgtcggc aatgtcgtgg cgccgcagac ggttatcgcg
     3421 caatccggcg ccgatattct gcgcttctgg gtcgcggcct cggattattc cgacgatctg
     3481 cgtatcggcc cggaaatttt gaagaccttc gtcgagacgt atcgcaagct gcgcaacacg
     3541 atccgctgga tgctcggctc gctggcgcat tacgacgcgg cggcgaagca ggtcattgtt
     3601 gcggacatgc cggagcttga acggttgatg ctgcatcggc ttgccgaact cgacagcgac
     3661 attcgcgaag cctatgcgcg cttcgattac aagcgtgtcg ttgcgcggct ctcggtattt
     3721 ctgaataccg atctgtcggc gttctatttc gatatccgca aagacacgct ctattgcgat
     3781 ccgccgtcga gcctgaaacg ccatgcgtcg ctcgccgcga tcgagcagat tttccgcgcc
     3841 gtcaccttat ggcttgcgcc gatcctcgct ttcacctgcg aggaggcgtg gctttcgcgc
     3901 tatcccgatg caacatcagt gcatctcgaa gcctttccgc aaattcctgc ggaatggcgc
     3961 gacgatgcgc tggcagcgaa atgggagaaa atccgtcgcg tccggtccgt ggttacgggc
     4021 gcactggaga tcgagcgcgg gcagaaacgc atcggctcgt cgcttgaagc cgcgccggcc
     4081 gtccacatcg ccgatcccga tttgcgcgcg gcggttacgg cgactgactt tgccgaggtc
     4141 tgcatcacct cggatattgc cgttgaagcc ggggaggggc cagcggaagc tttccgcgtg
     4201 gcggatatcc ccggtgtcgc tgtcgtcccg gcgcgggctc cgggccgtaa atgcgcccgc
     4261 tcctggaaaa tctcgcccgg cgttggcagc gatgcggaat tcccggatgt gacgccgcgc
     4321 gacgcgcagg cgttgcgcga actcgtcgcg ctcgggcttt ggccatgagc gcccaggcgg
     4381 atggcccctt gccggtgagc aaagcgacgt catcgcccgt ccgggttcgg gtgatcggcc
     4441 tgctcaccgc gctcgccacg tttttcctcg atcaggccaa taagctttgg ctcattttcg
     4501 actatggcat cgcgacgcat cagccgatcc atctggcgcc cttcttcgac gtgatttttc
     4561 taaaaaatcc gggaatttcc tattcgctgc tgacggcaag ttccgctttc ggccgctggg
     4621 cgctgctcgc catcgcattg gtggcgaccg cgtgccttat tttatggctg tggcggacgc
     4681 gcttgatgct gaccggcttt ggcctggggc tgatcatcgg cggcgcggtc ggcaacgcgg
     4741 ccgatcggct ggcctacggg tatgttgctg atttttatca ctttcatgtc ggcagctttt
     4801 cctggtatgt tttcaatctc gcagacgtgg cgatctgtat cggggtgggt ctgctggtcc
     4861 ttgaagggtt gaccgaaaag ccccgggcaa atgatgcggt cgaataggcc ggcttgccaa
     4921 caaatcgccg tgaattgtgg gattatgaga gattgccgac agcggcgaag gagatgtgcc
     4981 ttgcccttcg attcacgtag aacccgcaac cttgttgccg caacgagtgg cgttgcgctt
     5041 gccgctttgt tggcagcaag tcccgcgcgc gctgatggcg atatgtggaa ttccatgctg
     5101 ggttgggtcg ggatggggca caaatcggct ccatcgaccg acgggcctga cgacggcgcg
     5161 atcgattaca cgccgaagcc ggcgcttgtc gtgccgccga agatggattt gccgccgccg
     5221 caggcttcga catcaacccc tgccgattgg ccgcatgacc cggacgccac cgcgcgcgca
     5281 aaagccgagg ctgattcgcg gcgtccggcg ccgtccagcg attcgacctc cgacaattcc
     5341 gccggggact ttgtggccga cgccgacaag gcgaagcccg acaacggcga gagcgaggct
     5401 tgttccaata cagccggcaa accgatctgc ttctatgggc cgtcgggcct tatgaatttc
     5461 gccaagggtt ggaatatggg gggtatgttc agcacccatg ccgaagatac gtcgaagttg
     5521 cggatcggct cggagccgcc gcgggaatat ctgatccagc cgccgccggg ctatcaaatg
     5581 ccgaaagtga aggacgagga cgacagcgac gcggattctg gtgccgatgc gtcaaaatac
     5641 gattcggtcg atgttggcgc gacgcgccgt catcatcaca gcagcaccga ttccagcacg
     5701 gcaaattaag cctttagcgg catagatcgg tggatacttc ggcgagccga aggggctagg
     5761 gcttgtccgg cgtccggcct agaagctttg ccatctcttc ctcgagcgaa accatcaggt
     5821 cttcggcgcc cggcgcggac gcgacggaga cgtgttcttt gggctccgcc gagacgggcg
     5881 gagctagctc gaattccgga gccggcttgt tttcgacgac aggaggcact ggcgtcgtcg
     5941 ggctcgggtc acgcggccgg attggctggg taaaactcgg gcccgctggg cgcgactgcg
     6001 gctgcgccgg gttttggttg gggcgccagc gcatgaaacc ctgggtcgta gcctgcgacg
     6061 gggcttgacc ctcctgtgca ggggcggttg tgggcttggc aggaagcatc gcctcagcac
     6121 tcggcagagc cggctcaatg gctactggcg acggtacggc aggtgttggc ggaaatggcg
     6181 caggcgccgg tttgacgtcg gggcgcaccg ggggcggggt ccgccgcggg ccgacgggcg
     6241 ttgcgagcgg tgcgcgggcg ataggttgaa ccggcgcggg agcggccgtt acgatggtcg
     6301 gggtgaccgg cggaatctgg cgtgccgtga ccgggtcttc ggcaggagcc tcaggcacga
     6361 agggtggcag cggtaaatgc ggctcgacgc ttgtcggctt cagcggctgc gccgcgggca
     6421 attgcatcgc ctcaaagtct ttttcgcgcg gcttatcgcg atctttgtcg cgctggcgcg
     6481 gctcgcgcgc ctcggcccga acgatctgcg actcaatcaa catatcgttg gggccgccga
     6541 tcatgatgag atgttcgacg ttgtcgcgcc gcacgatgac gagctggcgc tggcggtcga
     6601 gatcgaaagc atcgacgatg ccgagccgca attgccggcc gcgcgcaccg gccattctca
     6661 ggcggcggcc aaaagccaaa cgaaagacaa gcaaaaccag caatgccgcg acgatcagcg
     6721 caacggcagc cgcgccatag gccagagcct tgtttccact gagccaactc aagccttgca
     6781 tgagcttgtc taatccccgg tcttgggcgc gtctggccct tccagccgct ttaggccaat
     6841 tccacaatcg ggcgagtttg ccgaggttcc ggaactgtcc cgaattcaat tattctaaca
     6901 cgccgccaca ttgccgcgcg ggcgaggtta ataaatggtt aacgcgcgag gcgcgggcgc
     6961 ggcgcggacg accttaacgt cacgcaactt ttgcgcaagt gcgcctcaat ctcttcccgc
     7021 actgggtcaa gcgggaaaaa ggccatcgcg cgcattgcaa accaccacag cgggttccat
     7081 tctcccgaat tgcaggcagc gcgccgaatc gcggccgcgc cgcccatcat gaggtgacgt
     7141 ttgactgacc agccgaccac cgctgccttc gaccgcacgg agcgtgccgg cagcccgggc
     7201 cttgttctgc ttttcgcggc ttgtcttgtc ggccccatcg ccgccttttc cgttctgccg
     7261 aaggatcagg ccggcacgat ggtgatcggc cttttgacga tcctggcggt catcggaatt
     7321 ttcgcgctgt tcggctatgc ggtcggcttt tttcaatttt ctgggcaggc ggcacgcaat
     7381 gatttgacgc gtctcatctg cgacagcgag ccggaaggcg ccatcgtcac cgacaacgcc
     7441 ggcaaaaccc tttacgccaa cgaagcctat atgcggctct ccggtgcacg cgatgtaagc
     7501 cagctcaagg ccgtcgagcg gctgctctcg gggtcgtccg atgtttcgga atcgatttac
     7561 cggctggccc aagctgcgcg tgaaggcaaa cgcaataatg aggaattgcg actcgcgccg
     7621 ccgctgaccg gcgacagcga agtcggctgg tatcgcgtgc gcgtgcgccc gctgaatttt
     7681 tctggcgcta agcgcgccac gctttggagc gttgccgacg taacgcggga gcgcgagagg
     7741 catgaaaatg tctttcagga attacaacac gcgatcgatt ttctcgacca tgcgccggcc
     7801 ggctttttct cctgtgacgc gtcgggcgcc gtctcctaca tgaatgcgac gctggcggga
     7861 tggctcgact atgatctcgc gcaggtcggt tccggcggtc tcgaactcaa cgatttcgtc
     7921 gccggcggcg gcgccgttct gctcgcaagt gtcgccggcg cgccgggcga tgtcgttacc
     7981 gatcagttcg acatggaatt gaagcgccgc aacggtcaga gcctgcccgt gcggatgctg
     8041 caccgggttg cgttcggtca ggacggtttg cccggcgctt cgcgcacctt ggttttcaac
     8101 cgcgcgccgg gcgaggaacc ggcggaagat ctgcgcgccg cggaagtacg ttttgcgcgt
     8161 gtcttcctcg ccgcgccgat ggccatggcc atcgtcgatc gcaatggacg catcaaacgc
     8221 tcgaacacgg ccttcgccaa actgatgcca gaggcgctcc gcgctgccga ttcagcgcaa
     8281 cgcagcatcc ggggaggtat ttccgacacc gatcatcccg ccctcgatag cgcgattgcc
     8341 gccgccgcgg ggtcgaagac cgatatcccg cccgtcgacg tgcagctctc gggcaatcgt
     8401 tcggcgcgcc tccttttttc cgcgtccgac gaagccgacg gaccgggcgc gacgatctac
     8461 gcgctcgata cgaccgaact gcgtacactt aaggagaatt ttgcgcagtc gcagaaaatg
     8521 caggcgatcg gccagcttgc cggcggtgtc gcgcatgatt tcaacaatgt gctgaccgcg
     8581 atcattggct attccgacct gctgctcgcc aatcatcggc cgactgatcc gtcctttcag
     8641 gacattatgc agatcaagca gaacgccaat cgcgctgccg gtctcgtgcg gcaattgctc
     8701 gccttctcgc ggcggcagac gctgcggccg gaggttttgc agctcggcga tgtactctcg
     8761 gaattgcagc tgatgatgcg tcgcctcgtt ggcgagaagg
//
LOCUS       DASWOG010000151         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_260260,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000151 DASWOG010000000
VERSION     DASWOG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260260"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGB93_03070"
     CDS             <1..285
                     /locus_tag="VGB93_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0145139.1"
                     /translation="LVLDFVWPKRRILEIYLNIAEWGDDGIFGAEAASNAYFHEPAAD
                     LTLHQAALMAAVLPDPRDRDASDPDRRVLIHAHIVETRAAAGETSVACFK"
     gene            453..641
                     /gene="rpmF"
                     /locus_tag="VGB93_03075"
     CDS             453..641
                     /gene="rpmF"
                     /locus_tag="VGB93_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0145140.1"
                     /translation="MAVPKRKTSPMKRGFRRSADALKAPTYVEDKNSGELRRPHHIDL
                     KTGMYRGRQVLKVKSAEE"
     gene            complement(728..1453)
                     /gene="phbB"
                     /locus_tag="VGB93_03080"
     CDS             complement(728..1453)
                     /gene="phbB"
                     /locus_tag="VGB93_03080"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY0145141.1"
                     /translation="MARVAVVSGGTRGIGAAVSRALAKAGYRVASVYAGNDEAAAKFK
                     SETGIPVYKCDVSDYEACAAGIEKVGADLGPVEVLVNNAGITRDTMLHKMKPEQWYAV
                     INTNLNSLFNMTRPVIEGMRERGFGRIVNISSINGQKGQMGQTAYAASKAGDIGFTKS
                     LAQENAKKGITVNVIAPGYIATEMVKAVPKEVLEKNILPHIPTGRLGEVEEIARCVLF
                     LVSEEAGFITGSTLSINGGQLML"
     gene            complement(1588..>1770)
                     /locus_tag="VGB93_03085"
     CDS             complement(1588..>1770)
                     /locus_tag="VGB93_03085"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022460.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0145142.1"
                     /translation="WDPGIVNVNGGAIAIGHPIGASGARVLVTLLHEMQRRGAHKGLA
                     TLCIGGGMGIALAVER"
BASE COUNT          370 a          562 c          473 g          365 t
ORIGIN      
        1 ctcgtcctcg atttcgtctg gccgaagcgc cgcatcctcg aaatctacct gaacatcgcc
       61 gaatggggcg acgatggcat tttcggcgcc gaggcggcgt ccaacgccta tttccatgaa
      121 ccggctgccg atctgacgtt gcatcaggcc gcgttgatgg ccgctgtcct ccccgatccg
      181 cgcgaccgtg acgcgtcgga tccagaccgt cgcgtcctca tccatgccca cattgtcgaa
      241 acacgcgccg ccgctggcga aacctccgtc gcctgtttca agtaaaattg tgcttcctga
      301 tcaaaaggtt ggccgataag gatattcagt gccgaaatag tttcggacgg aaaagcctgc
      361 gattttggga tagccagacc acgcattttt gtgtatcagc cactcaaccc attcgccggc
      421 agcttgccgg ccttgttatt tctggagctg aaatggctgt tccgaagcga aaaacctccc
      481 cgatgaagcg cggcttccgc cgctcggccg atgcgctgaa ggcgccaacc tatgtcgagg
      541 acaagaattc cggcgagctg cgccgcccac atcacatcga cctgaagacc ggcatgtatc
      601 gcggccgtca ggtgctgaag gtgaagtcgg cggaagaata agcttccgcc aaacacaatc
      661 cgaaaaagca cgatccgaaa aagtggaaac tggtttttcg aaaagatcgc actgatcttg
      721 gtggcggtca gagcatcagc tgaccgccgt tgatgcttaa agtcgagccg gtgatgaaac
      781 cggcctcttc cgaaactagg aagagcacgc agcgcgcgat ttcctcgacc tcgcccagcc
      841 ggccggtcgg aatatgcggc agaatgttct tctccagaac ttcctttggc accgccttca
      901 ccatctcggt cgcgatatag cccggcgcga tgacgttgac ggtgatgccc ttcttggcat
      961 tctcctgtgc cagcgatttg gtgaagccaa tatcgcctgc cttcgaggcc gcataggccg
     1021 tctgacccat ctgccccttt tggccattga tcgacgaaat attgacgata cggccgaagc
     1081 cgcgctcgcg cataccttcg atcaccggcc gcgtcatgtt gaacagcgaa ttcaaattgg
     1141 tgttgatgac ggcataccat tgctcgggct tcatcttgtg aagcatcgta tcgcgggtga
     1201 tgccggcatt gttgaccaga acctcgaccg gcccaaggtc cgccccgacc ttctcgatgc
     1261 cggcggcgca ggcttcataa tccgacacat cgcatttgta gacgggaatg ccggtttcgc
     1321 ttttgaactt cgcggcggct tcgtcattgc cggcatagac cgacgcgacg cgatatcccg
     1381 ccttcgccaa agcccgactc acggccgcgc cgatcccgcg tgtgcccccg ctcaccactg
     1441 ccacgcgcgc catgcgcccc tcccctcaac caatgccaat tccggcgctt ggcttagcaa
     1501 gcgattctaa gacaaatttc tagcccgtcg ccgtgtgcgc aaaaattcga ccttcggtcc
     1561 atgacatcgg tcaaagatgg aaagcgccta gcgttccacc gcgagtgcaa tgcccatgcc
     1621 gccgccgata caaagcgtcg caagaccttt atgcgcacca cgccgctgca tctcatgtag
     1681 gagagtgacg agaacgcgcg cgccggacgc gccaattgga tggccgatgg cgatcgcgcc
     1741 gccgttgaca ttgacgatac ccggatccca
//
LOCUS       DASWOG010000152         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_3647, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000152 DASWOG010000000
VERSION     DASWOG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3647"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..851
                     /locus_tag="VGB93_03090"
     CDS             177..851
                     /locus_tag="VGB93_03090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0145143.1"
                     /translation="MGTSISFKRPDGKDANGYLANAARGNAPGVVVIQEWWGLSEQIK
                     GLCDRFAVAGFDALAPDLYAGKVVPYHDTDAAGKEMNSLDFIDATTQTVRGAAQYLGK
                     NGAKVGLTGFCLGGAVTIIGAAKIPELTAGVVFYGIPPEAAAKPADIKIPLQAHFANK
                     DDWCTPQLVDAFEKGMKAAGKSLELFRYDAVHAFVNEQRQSVHDREAAELAWGRATEF
                     FRKHLG"
     gene            complement(1001..>1847)
                     /locus_tag="VGB93_03095"
     CDS             complement(1001..>1847)
                     /locus_tag="VGB93_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0145144.1"
                     /translation="VIDRSMPDGARFFGWEVADAAALDALAARLEQAEVSVTAEPKTL
                     ADARRVRALISFHDPAGNRLEAFYGAEIDDTPFSPGRSISGFRTGPLGLGHAVLTVEN
                     LEPVMAFYVDVLGFALSDYIEKPFRAYFFHINARHHSLALIETGRNGMHHLMVELFSL
                     DDVGQSYDIALTQPDRVNVTLGRHTNDLMTSFYAKSPSSFMIECGWGGREIEPATWNP
                     FEMHAGPSLWGHERVWLPPADREVASEMRMRAAASGLRAPVQVMDGNYTLMSGTCAWW
                     DGVKG"
BASE COUNT          357 a          569 c          580 g          341 t
ORIGIN      
        1 ttttttgttg cctatcgttc gtcattccgg ggcgatgcgc agcatcgaac ccggaatctc
       61 gagattccgg gctcgtcctt cggacgcccc ggaatgacgg tagtgtcaat tgcttcccgg
      121 ccttgtcgcg gactaaatag ccgcgaaagc cccgtcagaa accaccggag gaccccatgg
      181 gcaccagcat ttcgttcaag cgacctgacg gcaaggacgc caacggctat ctcgccaacg
      241 ccgcgcgcgg caacgcgccg ggcgtggtcg tgatccagga atggtggggg ctctccgagc
      301 agatcaaggg gctgtgcgac cgcttcgcgg tggctggctt tgatgcgctg gcgcccgatc
      361 tctatgccgg caaggtggtg ccgtatcacg acacggacgc ggccggcaaa gagatgaact
      421 cgctggattt catcgatgcc accacgcaga ccgtgcgcgg cgccgcgcaa tatctcggca
      481 agaacggcgc taaagttggt ctgaccggtt tctgcctcgg tggcgcggtc accatcatcg
      541 gcgccgccaa aattccggaa ctgaccgcgg gcgtggtgtt ctacggcatt ccgccggaag
      601 ccgccgccaa gcccgcagac atcaaaattc cactgcaggc ccatttcgcc aacaaggacg
      661 actggtgcac gccgcaactg gtcgatgcgt ttgaaaaagg catgaaggcc gccggcaagt
      721 cgctcgaact gtttcgctat gatgccgtcc acgccttcgt caacgaacag cggcagtcgg
      781 tgcatgaccg cgaagcggcg gaactggcgt ggggccgcgc gacggagttt tttaggaagc
      841 atttggggta ggttttttct cgtcattgct aggaacaaag cgacgaagca atccattctt
      901 tctttgagcc gcgctatgga ttgcttcgct tcgctcgcaa tgacgggata gagcgcggcg
      961 tttgggtccc tgcgttcgca gggacgacga ctgaatgtat tcagcccttc accccgtccc
     1021 accacgcaca cgtgcccgac atcagcgtgt aattgccgtc catcacctgc accggcgcgc
     1081 gcaggccgga agccgcggcg cgcatgcgca tctcgctggc gacttcgcga tctgccggcg
     1141 gcagccagac gcgctcatgg ccccagaggc tcgggccggc gtgcatctca aacggattcc
     1201 atgtcgcggg ctcgatctcg cggccgcccc agccgcattc gatcatgaat gatgacgggc
     1261 ttttggcata gaacgacgtc atgagatcgt tggtgtggcg gccgagcgtg acattgacgc
     1321 gatcgggctg ggtcagtgca atgtcatagg actggccgac atcgtcgagc gagaacaact
     1381 cgaccatcag atgatgcatg ccgttgcggc cagtctcgat cagggccagc gagtgatgcc
     1441 gcgcgttgat gtggaagaaa tacgcgcgaa acggcttttc gatatagtcg ctgagcgcga
     1501 atcctagcac gtcgacatag aacgccatga caggctcgag gttctcgacc gtcagtacgg
     1561 cgtgccccag cccgagcggc ccggtgcgga aaccggaaat cgaacggccg gggctgaacg
     1621 gggtatcgtc gatctcggcg ccgtaaaacg cctccagccg attgcccgcg ggatcgtgaa
     1681 acgaaatcag ggcgcgaacg cgcctcgcat cggccagcgt tttcggttcg gcggtgacgc
     1741 taacttccgc ctgttccagc cgtgcggcga gggcgtcgag cgcggctgca tcggccactt
     1801 cccagccaaa gaaacgcgcg ccgtccggca tcgagcggtc gatcacg
//
LOCUS       DASWOG010000153         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_131933,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000153 DASWOG010000000
VERSION     DASWOG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131933"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VGB93_03100"
     CDS             complement(<1..423)
                     /locus_tag="VGB93_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0145145.1"
                     /translation="MTTAFEAAAKDDPATMLMIARKLHNGGQYDDAAELCRRALHLAP
                     DDGWLKAAVKDLVTAHIPKWHFKIFGDALRNSAYDTALRRAVAPGARVLEIGTGSGLL
                     AMMAARAGAREVITCEMDRQVAETARKIISRNGYTDRIK"
     gene            complement(428..1123)
                     /locus_tag="VGB93_03105"
     CDS             complement(428..1123)
                     /locus_tag="VGB93_03105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0145146.1"
                     /translation="MALLRLRKPPNIFQLDNHTGPDRYPALFQFAREHFERRMKLRLL
                     SFGCSTGEEVSALRHYFPEAAIVGIDINPHNIAICRSRVGPDSNTTFVVAGTTGGEDV
                     DSYDAIFCLSVLRHGGLRAAGTERCDHLIRFDDFERTVADFTRCLKPGGLLFIANANF
                     RLCDTAFANRFEVLLLEDHHAPDPRAPIFGRDNRRIKDIYYNELGFQKLGRCGLHHPE
                     PNAGLKEGDRRGS"
     gene            complement(1434..>2163)
                     /locus_tag="VGB93_03110"
     CDS             complement(1434..>2163)
                     /locus_tag="VGB93_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY0145147.1"
                     /translation="APLLEFCDALPATPFLQSRRLAFRAVALSRLGRVDEARALVDVA
                     RHVMVFRFDPPSEHGDAVAFNARLAHWLIANTGSVPTPRPDCVIDYGLTHTRLPLINE
                     LRSFVRRSFATYVAQLPVLGLGDLMPLPPAGELFDFVVFLHGNGRNGEHVHPNAYVVG
                     VYYVQVPKDVQLASDMRGCLVLGACEKLTDGHQPAWGVRYLKPEPGMLVLFPAHMFHD
                     VVPTQSAEPRIVIGADVRPAPRHA"
BASE COUNT          447 a          621 c          682 g          413 t
ORIGIN      
        1 ttttatccga tcggtatacc cgtttcgaga aatgattttt cgggccgtct cggccacctg
       61 cctgtccatc tcacatgtga tgacttctcg tgcgcctgct cgggccgcca tcatcgcgag
      121 gaggccggag cctgtgccga tttcgagcac gcgcgcgcca ggcgccacgg cccgtcgcaa
      181 ggctgtgtcg taagcgctgt ttcgcaacgc atcgccgaaa atcttgaaat gccatttggg
      241 gatatgtgcg gtaacgagat ccttgacggc cgccttgagc caaccgtcgt cgggcgcaag
      301 gtgcagcgct cgcctgcaaa gttccgctgc atcgtcgtat tgtccgccat tgtgaagctt
      361 tctagcgatc atgagcattg tcgccggatc gtcttttgcc gcagcctcaa atgccgtggt
      421 caattttcta agatccacga cggtcgccct ctttcagtcc ggcgttcggc tcagggtgat
      481 gaagaccgca tcgacctaat ttttgaaatc ccagctcgtt atagtagatg tccttgatgc
      541 gccggttatc gcgcccgaag atgggggcgc gtggatcggg cgcgtgatga tcttcaagca
      601 gcaggacttc gaagcgattc gcaaaagcgg tgtcgcaaag tcggaaattg gcgttggcga
      661 tgaagagcag gccgcccggc ttgagacagc gggtaaagtc agcgaccgtg cgctcgaagt
      721 cgtcaaagcg gataagatga tcgcagcgct ccgtgccagc ggcgcgcaag ccaccatgcc
      781 gtaggaccga gaggcagaag atcgcgtcat aggagtcgac gtcttcgccg ccggtggtgc
      841 ccgcgaccac gaacgtcgtg ttggaatccg gaccgacgcg cgaccggcaa atcgcgatgt
      901 tgtgcggatt gatgtcgatg ccaacgatcg ccgcctccgg gaagtaatgg cgaagcgccg
      961 acacctcttc gcccgtggag cagccgaatg acaagaggcg caatttcatg cgccgttcaa
     1021 aatgctcccg ggcaaattgg aagagagccg ggtagcggtc cgggccggta tgattgtcga
     1081 gctggaaaat attcggcggc ttccggaggc gaaggagcgc catgctgcgc caacttccgt
     1141 ccgtcacaag tttgtacagg gcgcgtgcgg cgcgaaaagc caatgggcgt ttccgcggag
     1201 gcatgttggc acgcgccggc tgggctatac gcacggaccg aatgggacac tctccagcat
     1261 ggccgtatct acgtcggcgt ttgatcgcag tcgcggccga tccaagcgct ttcgctttta
     1321 tcggacaatt cgtctggtaa acgaaacctc ttcggcgacc gaaggtatca aatactgcca
     1381 caatcgcctt gttgcgtcgc gggctggaat tgcgggggcg ccgctgccgg attttaggcg
     1441 tgccgtggag ctggacggac atcggcgccg atcacgatgc ggggctcggc gctctgggtt
     1501 gggacgacat cgtgaaacat atgagccgga aaaagaacca acatacccgg ctcgggtttg
     1561 agatagcgca cgccccaagc tggctgatga ccgtcggtga gcttttcgca ggctcccaag
     1621 acgagacagc cgcgcatgtc tgatgccaac tgcacatcct tcggaacctg cacatagtag
     1681 acaccgacga cataagcgtt tgggtggacg tgttcgccgt tgcggccatt tccatgcagg
     1741 aagaccacga agtcgaagag ctcgcctgcc ggcggcagag gcatgagatc gccgaggccc
     1801 aacaccggaa gctgggcgac gtatgtggcg aaagatctgc gaacaaacga ccgaagctcg
     1861 ttgatcagag ggagccgtgt gtgcgttagc ccgtagtcga tcacgcaatc ggggcgtggc
     1921 gtcgggaccg aacccgtatt cgcgatcagc caatgggcca gacgcgcgtt gaacgccacg
     1981 gcgtcaccat gttccgaggg cggatcgaat cgaaagacca tcacatgccg cgcaacgtcc
     2041 acaagcgcgc gcgcttcatc gacgcggccc agccggctaa gtgcgactgc ccggaaggca
     2101 agtcgcctgg actggagaaa cggcgttgcg ggtagcgcgt cgcagaactc caacagtggc
     2161 gcc
//
LOCUS       DASWOG010000154         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_278418,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000154 DASWOG010000000
VERSION     DASWOG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278418"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGB93_03115"
     CDS             <1..188
                     /locus_tag="VGB93_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0145148.1"
                     /translation="IQGSVGKASFDEAKLIENIAAFVDAVAKAKPQGAKGTYIQRVAI
                     SSTMGPGVKVDPATVHA"
     gene            724..1239
                     /gene="rplJ"
                     /locus_tag="VGB93_03120"
     CDS             724..1239
                     /gene="rplJ"
                     /locus_tag="VGB93_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0145149.1"
                     /translation="MDRAEKKAAVTALNEVFSKTSVVVVAHYSGLTVAQMQNLRKQMR
                     AAGATVQVAKNRLAKIALEGTDTSSITSLLKGPTLLAYSQDPVAAAKVAVGFAKDNEK
                     LVILGGAMGKTALNPEAIRSLATLPSLDELRAKLVGLIQAPATKLAQLSTAPAAKLAR
                     VISAYANKDAA"
     gene            1294..1668
                     /gene="rplL"
                     /locus_tag="VGB93_03125"
     CDS             1294..1668
                     /gene="rplL"
                     /locus_tag="VGB93_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0145150.1"
                     /translation="MANLEKIVEDLSSLTVLEAAELAKLLEEKWGVSAAAVVAVAAGP
                     AAAAAAPVEEKTEFTVVLSAVGDKKIEVIKEVRAVTGLGLKEAKDLVEGAPKPVKEGV
                     TKEEAEKIKATLEKVGAKVELK"
     gene            1873..>5184
                     /gene="rpoB"
                     /locus_tag="VGB93_03130"
     CDS             1873..>5184
                     /gene="rpoB"
                     /locus_tag="VGB93_03130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0145151.1"
                     /translation="MAQPITQTFSGRKRVRKFFGHIREVAEMPNLIEVQKASYDQFLL
                     VNEPEGGRPNEGLESVFRSVFPISDFAGVSQLEFLKYVFEPPKYDVDECRQRGMTFAS
                     PLKVTLRLIVFDVDPETGAKSVKDIKEQDVYMGDMPFMTSNGTFVVNGTERVIVSQMH
                     RSPGVFFDHDKGKSHSSGKLLFAARIIPYRGSWLDIEFDAKDIVYARIDRRRKIPVTS
                     LLFALGMDGEEILSTFYNTVIYTRAGEGWRVPFDAERMKGVKATENMIDADTGEVAVE
                     AGKKLTVRAARQLVEKGLKAVRAQDIDLHGQYIATDLYNPSTGEIFAEAGDEITAKTL
                     PALIEAGFEELPILDIDHINIGPYIRNTLAVDKNSSREEALFDIYRVMRPGEPPTLDT
                     AEAMFHSLFFDDERYDLSAVGRVKMNMRLDLDAPDTMRVLRKADIVAVVRALVDLRDG
                     RGEIDDIDHLGNRRVRSVGELMENQYRLGLLRMERAIKERMSSVDIDTVMPQDLINAK
                     PAAAAVREFFGSSQLSQFMDQTNPLSEITHNRRLSALGPGGLTRERAGFEVRDVHPTH
                     YGRICPIETPEGPNIGLINSLATFARVNKYGFIESPYRRVKDNLVTDEVIYLSAMEES
                     KHYVAQANAPVDGQRRLTEDLVVCRHAGDVIMVPAERVDFMDVSPKQLVSVAAALIPF
                     LENDDANRALMGSNMQRQAVPLVKADAPFVGTGMEAIVARDSGAAIAARRTGYVDQID
                     ATRIVVRATEEADPSKPGVDIYRLLKFQRSNQSTCINQKPLVRVGDFVRKGDILADGP
                     STDLGDLALGRNVLVAFMPWNGYNFEDSILLNERIVKDDVFTSIHIDEFEVMARDTKL
                     GPEEITRDIPNVSEEALKNLDEAGIVYIGAEVQAGDILCGKITPKGESPMTPEEKLLR
                     AIFGEKASDVRDTSMRVPPGVQGTIVEVRVFNRHGVEKDERAQAIEREEIERLAKDRD
                     DELAILDRNVYSRLAELLSGKTAISGPKGFKKDTVLSRDILDAHPRSQWWIFAVEDDR
                     LMAETEAMRKQYDEAKKGLESRFLDKVEKLQRGDELPPGVMKMVKVFVAVKRKIQPGD
                     KMAGRHGNKGVVSKIV"
BASE COUNT         1062 a         1504 c         1607 g         1011 t
ORIGIN      
        1 tcattcaggg cagcgttggc aaggcttcgt tcgatgaagc gaagctcatc gagaatatcg
       61 ccgccttcgt cgatgccgtt gccaaggcca agccgcaggg cgcgaaaggc acctatattc
      121 aacgggttgc catctcgtcc acgatgggcc cgggcgtgaa ggtcgatccg gccaccgtcc
      181 atgcgtaaga gcgtccggaa ctgaatatcg gggcttcttg acaagctgaa aaaaagctga
      241 atcagcccct tggcatatta gggtcttgcg tttaaataga cagctcgggg ttgttggctc
      301 tgccgcaacc ccgaacccat ttagcttccg cgtctcgaca cgcagtcttt gattaggcgg
      361 cgtgaagaag gcgcggcgga atgaccggcg aaccggcgga tttcgatcca cctggtccct
      421 ccggtcatgt cctgtccgag actgccggtg tccatatggg gttttcgaac tccacaagga
      481 cttaatgtcg aagacgcgct tccggttccg gtgtttcggt ttcggattgc acgttcgaga
      541 tgccagcata gacggggtcg aaacggaata cgaagcgcag gtgcggcagt tcctgcagcc
      601 tttgattttg gtcgaatccc tctgtccggg acgcggggcg ttccggagga caggcgtagc
      661 gccgtgcgac aagccggcta acgcaaccaa gcgggcgaat gcctgcgaga cagagagagt
      721 attgtggaca gagctgagaa gaaagccgct gtcaccgctc tgaacgaggt ctttagcaag
      781 acatcggttg tggtggtggc gcattattcc ggcttgacgg tggcccagat gcaaaatctg
      841 cgtaagcaga tgcgcgccgc gggcgccacg gttcaagtcg caaagaatcg ccttgccaag
      901 atcgccctgg aagggaccga cacaagctcg atcacgagcc tgctgaaggg accaaccctg
      961 cttgcctatt cgcaggatcc ggtggcggcc gccaaggtcg ccgtcggctt tgccaaggat
     1021 aacgagaagc tcgtgatcct cggtggcgcg atgggaaaaa ccgcgctcaa tccggaggcc
     1081 attaggtcgc ttgcgacttt gccgtctctg gacgaattgc gcgccaagct tgtcgggctc
     1141 atccaggcgc cggcgacaaa actcgcgcag ctttcgaccg caccggctgc caagctggcg
     1201 cgcgtcatct ctgcctatgc gaacaaggac gccgcctgat ggccgtcttc aaattttcag
     1261 ctattcacga ccaaattcga aggaaaactt gcaatggcca atcttgaaaa aatcgtcgaa
     1321 gatctttcga gcctgacggt tctcgaagcc gcggaactcg ccaagctgct tgaagaaaag
     1381 tggggcgtat cggccgctgc tgtggttgcc gtcgctgcgg gcccggctgc cgctgccgcg
     1441 gctccggtcg aagagaagac cgagttcacg gtcgttctct ctgccgtcgg cgacaagaag
     1501 atcgaagtta ttaaggaagt tcgcgccgtc accggccttg gcctgaagga agccaaggat
     1561 ctcgtcgaag gcgcgccgaa gcccgtcaag gaaggcgtga ccaaagagga agccgagaag
     1621 atcaaggcga ctctcgagaa ggtcggcgcg aaggtcgagc ttaagtgagt tgcggcggga
     1681 tcgcgcgcga tcccgttttt cgatcgagcg gctccgaggc gtgcttcggg gccgttcagc
     1741 cgttccttat ccggctggtc gattgaattt tctgatgggg aagggcgcaa aacaccctgc
     1801 ggacgtctgt ggccgcgcaa tgttttcggg atgcatgcgg catcctgcgc aaactaacct
     1861 gcgaggagca cgatggccca acccataacg caaactttct ctggccggaa gcgggttcgc
     1921 aagtttttcg gacacattcg cgaagtggcg gaaatgccga acctcatcga ggttcagaag
     1981 gcatcctacg atcaattttt gctcgttaac gagcccgagg gcggccgccc gaacgaaggc
     2041 cttgagtcgg tgttccgctc ggtcttcccg atttcggatt tcgcgggcgt gtcgcagctc
     2101 gagttcctga aatacgtctt cgaaccgccg aagtacgatg ttgacgagtg tcgtcagcgc
     2161 ggcatgacgt tcgcttcgcc gctgaaagtc acgctgcgcc tcatcgtatt tgatgtcgat
     2221 ccggaaaccg gcgccaagtc cgtcaaggac atcaaggagc aggacgtcta tatgggcgat
     2281 atgcccttta tgacgtcgaa cggcaccttt gtcgtcaacg gcaccgagcg tgtcatcgtc
     2341 tcgcagatgc atcgatcgcc gggcgttttc ttcgatcacg acaagggcaa gagccactcc
     2401 tcgggcaagc tgcttttcgc ggcgcgcatc attccgtatc gcggctcctg gcttgatatc
     2461 gagttcgacg ccaaggacat cgtctatgcg cgtatcgatc gtcgccggaa aatcccggtg
     2521 acgtcgctgc tttttgcgct tggcatggat ggcgaagaaa tcctcagcac gttctataac
     2581 accgtcatct atacgcgggc cggtgaaggc tggcgcgtgc ccttcgacgc agagcggatg
     2641 aagggcgtca aggccaccga aaatatgatc gacgccgata ccggcgaagt cgcggtcgag
     2701 gcgggcaaga aactcaccgt ccgcgccgcg cgccaattgg tcgaaaaggg cctcaaggcg
     2761 gtccgcgcgc aggacataga cctgcatggc caatatatcg ctaccgatct ctacaatccc
     2821 agcaccggcg aaattttcgc cgaggccggt gacgagatca ccgcgaagac tctgccggcc
     2881 ttgatcgagg cgggcttcga ggaactcccg atactcgaca tcgaccacat caatatcggc
     2941 ccctatatcc gcaacacgct cgcggtcgac aagaactcct cgcgcgaaga agcactgttc
     3001 gacatctacc gcgtcatgcg tccgggcgag ccgccgactc tcgatacggc ggaggcgatg
     3061 ttccattcgc tcttcttcga cgacgagcgc tacgatctct ccgccgtcgg ccgcgtcaag
     3121 atgaacatgc gcctcgatct cgatgcgccg gatacgatgc gcgtcctgcg caaggccgat
     3181 atcgttgcgg tcgtgcgggc gcttgtcgat ctgcgcgacg gacgtggcga gatcgacgac
     3241 atcgatcacc tcggcaaccg gcgtgtgcgc tcggtcggcg aattgatgga aaatcaatat
     3301 cggctcggct tgctgcgtat ggagcgcgcg atcaaggagc gtatgtcctc ggtcgatatc
     3361 gacacggtca tgccgcagga tttgatcaat gcgaagccgg cagcggcggc tgtgcgcgaa
     3421 ttcttcggtt cgtcgcagct ctcgcagttc atggatcaga ccaatccgct gtcggagatc
     3481 acccacaacc ggcgcctctc ggcgcttggc ccgggtggtt tgacgcgcga gcgcgccggc
     3541 ttcgaggtgc gcgacgtgca tccaacgcat tacggccgca tctgcccgat cgagacgccg
     3601 gaaggtccga acatcggcct gatcaattcg cttgccacgt ttgcgcgggt gaacaagtac
     3661 ggcttcatcg aatcgccgta tcgccgcgtc aaggacaact tggtgacgga tgaggtcatc
     3721 tatctgtcgg cgatggaaga gtcgaagcat tatgtcgctc aggccaatgc gccggttgat
     3781 ggacagcgcc gtctgacgga ggacctcgtt gtctgccgtc atgccggcga cgtcatcatg
     3841 gtcccggccg aacgcgttga cttcatggac gtgtcgccga agcagcttgt ctcggtggcc
     3901 gcggcgctca ttccgttcct tgagaacgat gacgcgaacc gcgcgctgat gggctcgaac
     3961 atgcagcgcc aggcggtgcc gctcgtgaag gccgatgcgc cgtttgtcgg caccggcatg
     4021 gaagcgatcg ttgcgcgcga ttcgggcgct gcgattgcgg cacgccggac cggttatgtc
     4081 gatcagatcg acgcgacccg tatcgtcgtg cgcgcgaccg aagaggccga ccccagcaag
     4141 cccggcgtcg acatctaccg gttgctcaag ttccagcgtt cgaaccagtc gacctgcatc
     4201 aaccagaagc cgctggtacg ggttggcgat ttcgttcgca agggtgacat tctcgctgac
     4261 ggtccttcga ccgatctcgg cgatctcgcg ctgggccgca acgtgctcgt cgcgttcatg
     4321 ccctggaatg gctacaactt cgaggactcg atcctgctca atgagaggat cgtcaaggac
     4381 gacgttttca cctcaattca catcgatgaa ttcgaggtga tggcgcgcga caccaagctt
     4441 ggcccggaag aaatcacgcg tgacattccc aacgtctcgg aagaggcgct gaagaatctc
     4501 gacgaggctg gcatcgtcta tatcggcgcg gaagtgcagg cgggcgacat cctctgtggc
     4561 aagatcacgc cgaagggcga aagcccgatg acgccggaag aaaagctgct gcgcgccatc
     4621 ttcggtgaaa aggcgtccga cgtgcgtgac acgtcgatgc gtgtcccgcc gggcgtccag
     4681 ggcacgatcg tcgaagtgcg tgtcttcaat cggcatggtg tcgagaagga cgagcgcgcc
     4741 caggcgatcg agcgtgagga aatcgagcgg ctcgccaagg accgcgacga cgagctcgcg
     4801 atcctcgacc gcaacgtcta tagccgcctc gcggaattgc tctcgggcaa gacggcgatc
     4861 tccggcccga agggcttcaa gaaggatacg gtcctttcgc gcgatatcct cgacgcccac
     4921 ccgcgttcgc aatggtggat ctttgccgtc gaggacgaca ggctcatggc cgagaccgag
     4981 gccatgcgta agcaatatga cgaggccaag aaggggctgg aaagccgctt cctcgacaag
     5041 gtcgagaagt tgcagcgtgg cgatgaattg ccgcccggcg tgatgaagat ggtgaaagtc
     5101 ttcgtcgcgg tgaagcgcaa gattcagccg ggcgacaaga tggccggccg tcacggcaac
     5161 aagggtgtcg tttcgaagat cgtg
//
LOCUS       DASWOG010000155         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58430,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000155 DASWOG010000000
VERSION     DASWOG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58430"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="VGB93_03135"
     CDS             <1..2305
                     /locus_tag="VGB93_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0145152.1"
                     /translation="AGLLALFLTIVFVVKGAVIFPAAAALAWAVLAYVCIDFGFWDKI
                     FDTSEQADRIWRAGAETSLAATLLVFLFAYLNLNRWHVRASHVAMAWLAFLVALIGLA
                     IFDAPIAAGVARISLATIAAVGFILVLYLSTHGYDRAVMLIPTWFLLLVWVVAAGFTV
                     TGSLTNDLVSPALIGGLVLIVMLIGFTVMQNAFAGGGLGYGAINDSERKALALTGCGD
                     TIFDWDVNSDRVYVSPEVETQLGLYHSELEGPASKWLDVLHPFERDRYRASLDTMLEQ
                     RRGRINQEFRLRAADGHYFWFLLKARPVIGPEGEVIRVIGTLSDITEQKTAQTRLLHD
                     AVNDNLTGLPNRELFFDRLDAALKLSQTDALIRPTVICIDIDRFKHINETIGFSAGDS
                     ILLTVARRLGRILKPQDTLARLSSDQFAAVVISESETDHIIAIANVVRRAVSTPVSFG
                     ETEVPLTASIGLALFDPQLHANREDMLNDAEIAVRHGKRNGGNRIEVFRPSMRTVRSD
                     RLTLETDLRRALDRGEMQILFQPIIRLEDRTVAGFEALLRWDHPRLGRLSPSEFIPIA
                     EETGSIVDLGLFAMERTARELAAWQSALDLDPPLFASVNISSRQLLRHDLLQDVRAVL
                     SRWKIARGTLKLELTESLVMENPEYAAQILNRIHEFGAGLSLDDFGTGYSSLAYLQRF
                     PFDTIKIDRSFVQHNRKGARPVILRSIVTLAHDLGMDIVAEGAETESDAIELYQLGCE
                     FAQGYVFGHPMSASDARKLVGAAQAAA"
     gene            complement(2308..2919)
                     /locus_tag="VGB93_03140"
     CDS             complement(2308..2919)
                     /locus_tag="VGB93_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY0145153.1"
                     /translation="MMATAKAKGKDNEYLDGQFLLAMPGMADDRFARSVVYLCAHSDE
                     GAMGIVINRRAPSLNFSELLIQLEVIKPDEAIQLPMQAGAVPVLRGGPVEAGRGFVLH
                     SNDFYIDNSTLPIDGGVSLTATVDILRAIANGSGPDHAILALGYAGWSPGQLEDEMQN
                     NGWLTCPADAALIFETPLEERYGMAMRKIGIDPGFLSADAGHA"
BASE COUNT          569 a          922 c          882 g          684 t
ORIGIN      
        1 cgccggcctt ctggcgctct tcctgacgat cgtcttcgtg gtcaaaggcg cggtgatctt
       61 tcccgccgcc gcggcgctgg cctgggcggt gctggcctac gtctgcatcg acttcggttt
      121 ctgggacaag attttcgaca cgtcggagca ggccgatcga atctggcgcg ccggcgctga
      181 aacttcgctc gccgcgacgc tgctcgtctt tctcttcgcc tatctcaatc tcaatcgctg
      241 gcatgtccgt gcttcgcatg tggcgatggc gtggctggca tttctcgttg ccttgatcgg
      301 gctggcgatc ttcgatgcgc cgattgcggc gggcgttgcg cggatttcgc tcgcgaccat
      361 tgccgcggtc ggcttcatcc tggtgcttta tctttcgacc cacggctacg atcgcgcggt
      421 gatgctgatc ccgacctggt ttttgctgct cgtctgggtc gtcgccgcgg gtttcaccgt
      481 cacgggctca ctcaccaacg atcttgtgtc tcccgcgctg attggtggtc tggtcctgat
      541 cgtaatgctg atcggcttca cggtgatgca gaacgccttt gccggaggtg gcctcggcta
      601 tggcgcaatc aacgattccg agcgcaaagc cttagcgctt accggctgcg gcgatacaat
      661 cttcgattgg gacgtcaact ccgaccgtgt ctatgtcagc cccgaggttg aaacgcagct
      721 tgggctttat cacagcgaac ttgaagggcc ggcgtcgaaa tggctcgacg tcctgcatcc
      781 attcgaacgc gatcgctacc gcgccagcct cgataccatg ctcgagcaaa gacgcgggcg
      841 catcaatcaa gaatttcggc tgcgtgccgc cgacggacat tatttctggt tcttgctcaa
      901 ggcgcgtccg gtaatcggac cggaaggcga agtgatccgc gtcatcggca cgctttccga
      961 tatcactgag cagaagacgg cgcagacgcg gcttttacac gatgccgtga acgacaatct
     1021 gaccggcctg ccgaaccgcg aattgttctt cgatcggctc gacgccgcgc tcaaactttc
     1081 gcaaaccgac gcgctgatac ggccgactgt catctgcatc gatatcgacc gcttcaaaca
     1141 tatcaacgaa accatcggat tttcggccgg tgattcaatt ttgctcacgg tcgcgcgccg
     1201 gcttggccgc attttgaagc cgcaggacac acttgcgcgg cttagcagcg atcagttcgc
     1261 cgcggtcgtg atctcggaga gcgagacaga ccatatcatt gccatcgcca atgtcgtgcg
     1321 ccgcgcggtt tcgactcccg tttccttcgg cgagaccgaa gtgccgctca cggcttcgat
     1381 cggccttgcc ttgttcgacc cgcaattgca cgccaatcgg gaagatatgc tcaacgacgc
     1441 tgagatcgct gtgcgtcatg gcaaacgcaa cggcggcaat cgcatcgaag tcttccggcc
     1501 ctcgatgcgc accgtgcgat cggatcggct gacgcttgag accgacttgc gccgtgcgct
     1561 tgaccgcggc gagatgcaga ttctatttca gccgatcatt cggcttgaag atcgcacggt
     1621 cgccggtttc gaggccttat tgcgctggga tcacccacgg ctcggacgcc tcagcccatc
     1681 cgaattcatt ccgattgcgg aagagacggg tagcattgtc gatctcggtc tttttgcgat
     1741 ggaacgcacg gcgcgcgaac ttgcggcctg gcaatcggcg cttgatctcg atccgccgct
     1801 tttcgccagc gtgaacatct cgtcgcgcca attgctgcgt cacgatctct tgcaggacgt
     1861 acgcgctgtg ctttcacgct ggaagatcgc gcgtggcacg ctgaaactgg agttgaccga
     1921 aagcctcgtg atggaaaatc ccgaatatgc ggcgcagatt ctcaaccgca tccatgagtt
     1981 cggcgccggc ctttcgctcg acgatttcgg caccggctat tcgtcgctcg cttatctgca
     2041 gcgcttcccc ttcgacacga tcaagatcga ccgttccttc gtgcagcata accgcaaggg
     2101 tgcgcgtccc gttatcctgc gctccatcgt gaccttggcg catgatctcg ggatggacat
     2161 tgtcgcggaa ggcgcggaga ccgaatccga cgcgatcgag ctttaccagc ttggttgcga
     2221 attcgcgcag ggttacgtct tcggccatcc gatgagcgcg agcgatgccc gtaagcttgt
     2281 cggtgcggcg caggcggcag cctgaattta agcgtggccc gcatcagctg agagaaagcc
     2341 gggatcgatg ccaatcttgc gcatcgccat cccataacgt tcttccagcg gcgtctcgaa
     2401 gatgagggcc gcgtcggcgg ggcaggtgag ccagccgttg ttttgcatct cgtcttccaa
     2461 ttgacctggc gaccaaccag cgtagccaag cgcgagaatc gcgtgatcgg ggccggagcc
     2521 gttcgcaatc gcgcgcaaaa tatcgaccgt cgcggtcagt gacacgccgc cgtcgatcgg
     2581 cagtgtcgaa ttgtcgatat agaaatcgtt tgaatggaga acgaagccac ggccggcctc
     2641 gaccgggccg ccgcgcaaaa ccgggacggc gcccgcctgc atgggcagtt ggatcgcttc
     2701 atccggcttg atgacttcaa gctgaatgag aagctcggaa aagttgaggc tgggcgcgcg
     2761 gcggttgatg acgatcccca tcgcgccttc gtcagaatgt gcacaaagat aaacgacgct
     2821 gcgggcgaaa cggtcatccg ccattccggg cattgccagg aggaattgcc cgtcgagata
     2881 ttcgttatct ttacccttcg ccttcgccgt tgccatcatc aagactctta ccaagtgagg
     2941 aacaaatagg ttccgaaagc cacaatttca tcttttgcca atgctaaatt agcaagaagg
     3001 aaaacgcgct cgtggttgca agccttctac gtaatcttcg ttccttaggt tccaagc
//
LOCUS       DASWOG010000156         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_22026,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000156 DASWOG010000000
VERSION     DASWOG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22026"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB93_03145"
     CDS             <1..186
                     /locus_tag="VGB93_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145154.1"
                     /translation="RPWHAYPNYVTRVPYGWPVIAYSRAYSYVPGVSYPVPVRVYYIP
                     QTEPRYNVPPYAVVAPY"
     gene            complement(180..992)
                     /locus_tag="VGB93_03150"
     CDS             complement(180..992)
                     /locus_tag="VGB93_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0145155.1"
                     /translation="MTHASLPAWMPLSVLTWIVVMVVFHGIGYGFEYCDRVGLFKSAK
                     VRQADKLTYQQLLPRALFNQVFVLLPAMVLVQWLGWAFVGAAHLSVWRFLGMLVGMAI
                     GHDVVQYVTHRYVLHNPRTSRFLGHTVHHMTGASKAISALYMTPADFFLEIVLPYLLP
                     LVVLGGGGSDILFHLLVAGLGAFGGLYEHSGYDFAAPLRQTRFFKSAPWLGAILETLV
                     SSHAHGQHHTRGVVSFSDGFGSPGICDTVFATRWDKVEERPRVRNARPGRAQ"
     gene            1292..1987
                     /locus_tag="VGB93_03155"
     CDS             1292..1987
                     /locus_tag="VGB93_03155"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0145156.1"
                     /translation="MSLVSPEKQNMARQTARLLLEVAAISVNTEKPFIFTSGWASPVY
                     TDMRKIIAYPRLRQTLTEFAAHTIIAEIGCESLDIIAGGETAGIPFAAWLAERLMLPM
                     QYVRKKPKGFGRQAQIEGNLIDNGRALLVEDLATDGRSKVNFCEALRNAGQRCDHAFV
                     YFFYDIFPQARSLMQEIGVNLHYLVTWWDVLEAAHRDGSFPPSVLSEVEKFLHEPAAW
                     SAAHGGVSEFKPA"
     gene            2114..2698
                     /locus_tag="VGB93_03160"
     CDS             2114..2698
                     /locus_tag="VGB93_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145157.1"
                     /translation="MAETSKIFSFFGPGVTQRGVLISGSLALAGMVLSFAPVLPQPSL
                     WQVLGTPAVAPAHASTPNSTWAGHLESPGTSGTDVGPSHALTSASYTTGFDSAAAAPL
                     PATSLKTAVVDPGACPNDLNCTFRSKTGATLPPPHARMASIGSSRPHAGTTHQDLKKP
                     TGLAALLPSLPSTHTLLKPFAFVANTFGGLMHRL"
     gene            complement(2715..3833)
                     /locus_tag="VGB93_03165"
     CDS             complement(2715..3833)
                     /locus_tag="VGB93_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEY0145158.1"
                     /translation="MRSRIYFFWAIVASSLAFVQLMAANLPTKEDTRLQPIFQSNRVW
                     NAVTTTHDGRAFVGFPGADGPGTEVEELSPDGQGKPYPDAAWNNWQPGHDPANVFVHV
                     NALRIGPDGALWIVDAGAPGFGKPSVPGAARIFRFTLHDNKPARVYSLASAVTPQSYI
                     DDIRFNGRMAYISDAGAPALIVLDLESGEVRRVLDGDSSVTDKRPMYADGKLIRTADG
                     KPLHIQADQIEVSPDGQYLYCQPASGPLARVDTRYLDDPHFSAKDLAAHVEKNWVDTP
                     TTGGTAIDANGTIYLSDVNKRRILTISPDGKVSTLISDPRLIWVDAMWIDRDGYLWIP
                     ATQLNLTPGLNAGRQKVHYPVFIYKMKIDVGPPPRDHS"
BASE COUNT          812 a         1263 c         1214 g          821 t
ORIGIN      
        1 cgcccgtggc acgcctaccc gaactatgtc acccgtgtgc cttacggctg gccggtgatc
       61 gcctattcgc gcgcctattc ctatgtgccg ggtgtgtcct accccgtgcc tgttagggtc
      121 tattacatcc cgcagacgga accgcgctac aacgtgccgc cttatgcggt cgtcgcccct
      181 tactgagcgc gacccggccg cgcattgcgc acgcgcgggc gttcctcaac cttgtcccaa
      241 cgggtcgcaa aaaccgtgtc gcaaatgccg gggctgccga agccgtcgct gaagctgaca
      301 acgccgcgcg tatgatgctg gccgtgcgcg tgactcgaga cgagcgtctc cagaattgca
      361 ccgagccagg gggccgactt gaagaagcgc gtttgccgca acggcgcggc gaaatcgtag
      421 cccgaatgtt cgtagaggcc tccaaaagcc ccgaggccgg cgacgaggag atgaaataaa
      481 atatcgctgc cgccaccacc gaggacgacc agcggcaaaa gatagggcaa cacgatctcg
      541 aggaaaaagt cggcgggcgt catatagagc gcgctgatcg ccttgctcgc ccccgtcatg
      601 tggtggacgg tatggcctag gaatcgcgag gtccggggat tgtgcagcac atagcggtgc
      661 gtgacatatt ggacgacgtc gtggccgatc gccatgccga ccagcatccc gagaaagcgc
      721 cagacgctga gatgcgcggc cccaacaaag gcccatccga gccattgcac caggaccatc
      781 gctggcagca gcacgaaaac ctgattgaac aacgcccgcg gcagaagctg ctgataggtg
      841 agcttgtcgg cctggcgcac cttggcgctt ttgaaaagcc ccacacggtc gcaatattcg
      901 aatccgtagc cgatgccgtg gaagaccacc atcacgacga tccaggtgag gaccgagagc
      961 ggcatccagg ccggcaagga agcatgcgtc atgcgaatta cccgttcgcg gcgaaccgcc
     1021 ctgcatttct atggccgctt ggcgcccggc gggcaagcca tagaacgacg ccgattgagg
     1081 atagccgatc acgaatgatt ttcgactgaa acactttaga atcatcatga tctgccgcaa
     1141 gttttgtcaa attcggtcgg ccacgcagcg tgttccagtt tcccggtcat gcccttggcg
     1201 cgcgatatcc gctggaaatc gccatttcgg ggttgacgct ccgccctgca aaagcgacga
     1261 cgattttccc tctttggata tggtctcaag catgtcgctg gtttcgccgg aaaagcaaaa
     1321 tatggcccgc cagaccgcgc ggctcctcct cgaagtagcg gccatttcgg tcaataccga
     1381 aaagcccttc atcttcacgt cgggttgggc gagccccgtc tatacggaca tgcgcaaaat
     1441 cattgcctat ccgcgcctgc ggcagaccct gacggaattt gccgcccaca ccattatcgc
     1501 ggagatcggt tgcgaaagcc tcgacatcat cgccggcggc gagacagcgg gcatcccctt
     1561 tgccgcgtgg ctggccgaaa ggttgatgct gccaatgcaa tatgtgcgca agaaaccgaa
     1621 gggctttggg cgccaggcgc aaatcgaggg caatctgatc gacaacggcc gggctctgct
     1681 cgtcgaggat ctcgcaaccg acgggcgcag caaggtcaat ttctgcgagg ccttgcggaa
     1741 cgcggggcag cgctgcgacc atgccttcgt ttattttttc tacgatattt ttccgcaggc
     1801 ccgctcgctg atgcaggaga tcggcgtcaa tctgcattac ctcgtaacct ggtgggacgt
     1861 gcttgaagcg gcgcaccgcg acggctcttt cccgccatcg gtcctcagcg aagttgaaaa
     1921 attcctgcac gaaccggccg cttggtcggc cgcgcatggg ggcgtttccg aattcaagcc
     1981 agcttaggct gagggattcc tgcaacagcc ggggcagaat ttcttgctgc cctttttcgg
     2041 catcggtctt gcctaggtct tgacgcaata tttgcgttcc attctcgcgt cgcagaaatc
     2101 tggcgagact ttgatggccg aaacttcgaa aatcttctcg ttttttggac cgggcgtgac
     2161 gcagcgtggc gttctcatct cgggcagtct cgctttggcg ggcatggttt taagctttgc
     2221 gcctgttttg ccgcagccaa gcctctggca ggttttgggc acgcccgcag tcgcgcccgc
     2281 ccatgcatcg acgccgaatt caacctgggc cggccatctc gaaagccctg gcacgagtgg
     2341 gacggatgtt ggcccaagcc atgcgctgac ctcggcctct tacacgaccg gcttcgatag
     2401 tgccgccgcc gccccgttgc cggccacctc gctcaagacg gcggtcgtcg atccgggcgc
     2461 ctgccccaac gatctcaact gcaccttccg gtcaaagact ggcgcgactt tgcccccgcc
     2521 gcacgcacgg atggcatcta tcggatcgtc ccgcccacac gcgggcacaa ctcatcagga
     2581 tttgaagaaa cccacgggtc tcgccgccct gctgccaagc ttgccctcga cgcatacgct
     2641 cctgaagccg ttcgccttcg tcgcgaatac gttcggcggc ctgatgcaca ggctctgagt
     2701 ccccttggca gatttcagga atggtctctc ggcggcggtc caacgtcgat cttcatcttg
     2761 tagataaaga cgggataatg gaccttctgg cgcccggcat tgagcccggg cgtcagattc
     2821 aactgcgtcg cgggaatcca taggtagcca tcgcggtcga tccacatggc gtcgacccag
     2881 ataagccgcg ggtcggagat cagggttgaa accttcccgt cggggctgat cgtcagaata
     2941 cgccgcttat tcacgtcgct gagatagatc gtgccattgg cgtcgatcgc cgtgccgccg
     3001 gtcgtcggcg tatcgaccca gtttttctcg acatgcgcgg cgaggtcctt tgctgaaaag
     3061 tgcgggtcgt cgaggtagcg cgtatcgaca cgtgccagcg gcccggacgc cggctggcag
     3121 tagagatact ggccgtcggg cgagacttcg atctggtcgg cttggatatg cagcggcttg
     3181 ccatccgcgg tgcggatcag tttgccatcg gcgtacattg gccgcttgtc agtcacggaa
     3241 gaatcgccgt cgagcacacg cctcacctcg ccgctttcga gatcgagcac gatcaacgcc
     3301 ggcgcgccag catcgctgat ataggccatg cggccattga agcggatgtc gtcgatatag
     3361 ctttgcgggg tgacagcaga cgcgagcgaa tagacccgcg caggtttgtt atcgtggagc
     3421 gtgaaacgga agatgcgtgc ggcgcctgga acgctaggtt tgccgaagcc aggcgcgccg
     3481 gcatcgacga tccagagcgc gccgtccggc ccgatccgca gcgcgttgac gtggacaaag
     3541 acgttcgcgg gatcgtgccc cggctgccaa ttgttccaag cggcatcggg ataaggtttg
     3601 ccctgcccat ccgggcttag ctcctcgacc tccgtgccag gaccgtccgc gccggggaag
     3661 ccgacaaagg cgcgtccatc atgcgttgtt gtgaccgcgt tccaaacccg gttcgactgg
     3721 aaaatcggct gcagccgggt gtcttctttg gtcggcaaat ttgccgccat gagctgcacg
     3781 aacgcgagcg atgatgcgac gatggcccag aagaaataga tgcggctgcg catcctcacg
     3841 tccccgattg catgactgtc agctgcacta tcacagtgca gatgaaccgc gcccatctgg
     3901 caagctggac gtcatctgaa attttggtga gggccgtcct cttagctcac ggtgtcaagt
     3961 tctcatggcg ccgcaacaaa aagcaataac acgttgaaat gcaatgcaat atttgatgat
     4021 tcgtatgact gaccgcgttt cctagcagag gcccggcttt agcctagcag cacgaggaca
     4081 tcctaccttc ggtactatca gccgaatcgc
//
LOCUS       DASWOG010000157         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_22039,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000157 DASWOG010000000
VERSION     DASWOG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22039"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGB93_03170"
     CDS             complement(<1..731)
                     /locus_tag="VGB93_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY0145159.1"
                     /translation="MTQTVRIAIDAMGGDHGPEVILPGAALALTQHADLSFTFFGDQT
                     RLKPLLAANPRLASVSSVRHTDVEIRMEDKPSQALRNGRRTSSMWQALEAVKRGEADA
                     AFSAGNTGALMAMAKICLHMIPAIDRPAIAVLWPTLRGESIVLDVGASIGADAQHLVD
                     LAIMGSAMARIVFSSLRPTVGLLNIGVEEVKGIEEVKAASRILRELNSPDFDYRGFVE
                     GDDIGKGSVDVVVTEGFAGNIALKTA"
     gene            complement(815..1372)
                     /locus_tag="VGB93_03175"
     CDS             complement(815..1372)
                     /locus_tag="VGB93_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY0145160.1"
                     /translation="MSKADSYAPPPKIFSHLILVDEVPEQGLDVTLSADAPTRKALAK
                     ADGLVGVAALDADFHVARQGLSSFNVTGTVRAKITQICVLTLEPFDSDLAEEVDVDFA
                     EASAAAQAATQADILLDAQSGDRDPPDPIEDGAIDLGALAAEFLALGLDPYPRRPGAE
                     FAPVTDPHDVIERPFDALKKLTDQS"
     gene            complement(1365..1922)
                     /locus_tag="VGB93_03180"
     CDS             complement(1365..1922)
                     /locus_tag="VGB93_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEY0145161.1"
                     /translation="MIFGLFRGKRNASVIGRLHADIVAAARDPVLFTDYGIADTIDGR
                     FESIALHAALALRRLKQLPPPGPEIAQDLADAIFRHFDIGLREMGISDTGVPRRMRDL
                     AEALLGRANAYNEALTQPGTAQHTALAAALSRNVFADARGGERLARYAERCDEALAQM
                     SLADFLKGPIFGVEAAANVREVCDE"
     gene            2126..2560
                     /locus_tag="VGB93_03185"
     CDS             2126..2560
                     /locus_tag="VGB93_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY0145162.1"
                     /translation="MSVALRLGLCAAMATGLSGCLGYDGDIVHGYQLDPTLLNQVKVG
                     SSAEQVLVVMGTPSTTSTVGGSAWYYITQKSDRQYAFQDQSITDQHVLAIYFDKDKKV
                     TRVANYGLKDGKIFDFVSQTTPTGGADSSVLNGMFKSLLRFN"
     gene            complement(2611..>3136)
                     /locus_tag="VGB93_03190"
     CDS             complement(2611..>3136)
                     /locus_tag="VGB93_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY0145163.1"
                     /translation="AMTAVGRAAGAVVEEVRRQFREKPGIMAGTERPDYGRAVDMLTK
                     AAIKEMIIPSLLPVLAPLVVYFGVLLISGSKASAFAALGASLLGVIVNGLFVAISMTS
                     GGGAWDNAKKSFEDGFVDKDGAKHLKGSDAHKASVTGDTVGDPYKDTAGPAVNPAIKI
                     TNIVALLLLAILAH"
BASE COUNT          625 a          969 c          968 g          574 t
ORIGIN      
        1 gcagtcttga gcgcgatatt gccggcaaag ccttcggtca cgaccacatc gacgctgcct
       61 ttgccaatgt cgtcaccttc gacaaagccg cgatagtcga aatcgggcga attgagttcg
      121 cgcaaaatcc gcgaggcggc cttcacttcc tcgatgcctt tgacttcttc gacaccgatg
      181 ttgagcagcc ccacggtcgg gcgtagcgac gagaagacga tccgcgccat cgccgagccc
      241 atgatcgcga gatcgacgag atgttgggca tcggcgccga tcgaggcgcc aacgtcgagg
      301 acgatcgatt ccccccgcag cgtcggccaa agcacggcaa tagccggccg gtcaatcgcc
      361 ggaatcatgt gcaggcaaat cttggccatc gccatgagcg cgccggtgtt acccgcggaa
      421 aaggcggcat cggcctcgcc ccttttgacg gcttcgagcg cctgccacat cgaagaggtg
      481 cgccggccgt tgcgcaacgc ctggctgggc ttgtcttcca tacggatttc gacatccgtg
      541 tgccgcacgc tcgagacgct ggcgagccgc ggattggctg caagcagggg tttcagccgc
      601 gtctgatcgc cgaaaaacgt gaaactgagg tcggcatgct gggtcagcgc gagcgcggcg
      661 ccaggtagga tcacctccgg gccgtggtca ccgcccatgg catcgatggc gatgcgcacc
      721 gtttgcgtca tgaaagctcc ctagcccggc cggaccgcca agctgcggcc tgatccgcta
      781 aggcaaagcg ccccgcccgg caagcgcggc cagatcaaga ttgatcggtg agcttcttga
      841 gcgcatcgaa cggccgctcg atcacatcgt gcggatcggt cacaggggcg aactctgccc
      901 ccggtctgcg cggataaggg tcgaggccga gcgcaagaaa ttccgccgcc agcgcgccaa
      961 ggtcgatcgc accgtcctcg atcgggtcgg gcgggtcgcg gtcacccgat tgggcgtcga
     1021 gcagaatatc ggcctgggtg gcggcttgcg ccgcggccga agcttcggcg aaatcgacat
     1081 cgacctcctc ggcaagatcg ctgtcgaaag gctccagcgt cagaacgcag atctgcgtga
     1141 tttttgcccg cacggtgccg gtgacattga agctggaaag cccctggcgc gcgacgtgga
     1201 agtccgcgtc gagcgctgca acgccgacga ggccgtcggc cttggccaat gccttccgcg
     1261 tgggtgcatc ggcgctcaac gtgacgtcga ggccctgctc cggcacttcg tccacaagaa
     1321 tgagatgcga aaaaatcttc ggcggcgggg cgtaggaatc ggctttactc atcacacacc
     1381 tcacggacgt tcgcagcggc ttcgacccca aatataggtc ctttgagaaa atctgcgagt
     1441 gacatttgcg ccagggcttc atcgcatcgc tcagcgtagc gagcaaggcg ctcgccgcct
     1501 cgcgcatcgg cgaagacatt gcgcgagaga gccgcagcca aggccgtatg ctgcgccgtg
     1561 cccggctgcg tgagcgcttc gttgtaagcg tttgcgcggc ccaaaagtgc ctcggcaaga
     1621 tcgcgcatcc gccgcgggac gccggtgtcg ctgatgccca tttcgcgtaa gccgatgtcg
     1681 aaatggcgaa agatggcatc ggcaagatcc tgcgcaattt cgggtcctgg cggcggcaat
     1741 tgcttcagac gccgcagcgc cagcgcagcg tgcaaagcaa tcgattcgaa gcgcccatcg
     1801 atcgtgtcgg caatgccgta atccgtgaac aaaaccgggt cgcgcgcggc ggcgacaatg
     1861 tcggcatgca gccgcccgat gacacttgca ttgcgtttgc cccggaaaag cccaaaaatc
     1921 atgactttag attccatatc gcggcttgag cggggcgagt tgcgaccttg atttccgcat
     1981 actagaaacg atcggattat acgcgtttgg tcgaatcggc caaaacgcgc cttgatccga
     2041 ggggccggag tttccaagaa tgatgtttgc aagtgcggcc gtaagagaag aaccgacggc
     2101 cgcccaaaag cgcagaacaa agctcttgag cgttgctctc cggctcgggc tttgcgctgc
     2161 catggccacg ggactctccg gctgtctggg ttacgatggc gatatcgtcc acggctatca
     2221 actcgacccc acgctgctca atcaggtcaa ggtcggctcg tcggccgagc aggtgctcgt
     2281 cgtgatgggc acgccatcca cgacatcgac cgtcggcggc agcgcctggt attacatcac
     2341 tcagaaatcc gaccggcaat atgcctttca ggatcagagc attacggacc agcatgtcct
     2401 ggcgatctat ttcgacaagg acaagaaggt cacgcgcgtc gccaattacg gtctgaagga
     2461 cgggaaaatc ttcgatttcg tgtcgcagac tacgccgacc ggcggcgccg attcgagcgt
     2521 cttgaacggc atgttcaaat ctctcttgcg gtttaactaa ataccagtcg atgagatggc
     2581 cggatcgctc cggccatctt cgcttgggtt ttagtgcgca aggatcgcca gcaagagcag
     2641 cgcgacgata ttggtgatct tgatcgccgg attgaccgca gggccagccg tgtccttgta
     2701 aggatcgccg accgtgtcgc ccgtcacaga tgccttgtgg gcgtcgctgc ccttcagatg
     2761 tttcgcgccg tccttatcga cgaaaccatc ttcgaagctc ttcttggcat tatcccaggc
     2821 gccgccgccg gacgtcatcg aaatggcgac gaagagaccg ttgacgatga cgccgaggag
     2881 cgaagcgccg agcgcggcga acgccgaggc cttcgagccc gaaatgagca gcacgccgaa
     2941 atagaccacg agcggcgcca gtaccggcag cagcgacggg atgatcattt ccttgatcgc
     3001 cgcctttgtc agcatatcga ccgcgcggcc atagtccggc cgctcggtgc cggccatgat
     3061 gccgggcttc tcgcgaaatt ggcggcgaac ctcctcgacg actgcaccgg cggcgcggcc
     3121 gacggcagtc atggcg
//
LOCUS       DASWOG010000158         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_223636,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000158 DASWOG010000000
VERSION     DASWOG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223636"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..3376)
                     /locus_tag="VGB93_03195"
                     /pseudo
     CDS             complement(151..3376)
                     /locus_tag="VGB93_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590110.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
BASE COUNT          687 a          938 c         1051 g          700 t
ORIGIN      
        1 ttcgaggtcg agaccgaccg tcgttcgggc aagccgtctg cggttaatct gcaagccgcc
       61 tgatcgtaaa gatttgaact gaaaagccgc cgttcctctg aacggcggct ttttgctggg
      121 tcaaagcgtc gaactgacgt ggccaccatt ttaatgaaaa ttccggcctt tcggcagaac
      181 gtggtggatg tcgtcgcgct tgtcggctgt ggtttgtccg gtctttgtgt caaataatgt
      241 cggcagtgtg tggcgcattt gattgcgcgg atgccgttgc ggatttttga ttggttgggt
      301 gaggctgttg tccggtcggg ataaaactcc gagcagcggt gtcagatcgt cgagccgttc
      361 gacgacgaca tgaatgacgc ccgattccga ttgcaatcgc ccggtgatgg cgacgaaacg
      421 cgcagcgatg acaatcgccc ggttggattc gaaaattttc ggccagacga tcgcattggc
      481 gatgcctgtt tcatcctcga tcgtcagaaa aatcacgccg cttgccgtgc ctggccgctg
      541 ccggatcagc acgagcccgg cggtacgtac gcgctgtcct gaggccatga gtgcgagatc
      601 ctgcgtgcgg acgatttttt cggtcttaag gtgcggcctc agaaagctga ccggatgggc
      661 ctttagcgat agcgacaaat gccggtaatc ctcgacgaca tgttcgccga gcgacatggg
      721 cggcagatgt gcctccggct ccagcgcgcg gaagcttaag tctttcaaca aggggaggtc
      781 gtccttgtcg cccgcccggt tcaagccgcg cacggcccag agcgcgtcac gtcggtcgag
      841 gccgagggag cggaaagcat cggcttcggc cagcgccgcg agaacgctcg gtgcaagacc
      901 tgtgcgcagc cagagatcac ggattgaatc atagcctttg ccgcgactga cgacgagcgc
      961 ttccatatcc gtttctgcga cgccctgaat ctggcgaaag ccaaggcgta atttatgcgt
     1021 ggaacggata tcgtgtcgca ttgaggcatg gcgcgaatga aggcgcgacg aacatgtggc
     1081 cgcctcatct tccagcgtgc aatcccattc ggaataattg atgtctattt cttcgaccgt
     1141 gacaccatgt ccgcgcgcat cgcggacgat ctgtgctggc gcatagaagc ccatgggctg
     1201 ggcattgagc aaggcgcaag cgaagacatc cggataacgg catttgatcc aggctgaagc
     1261 atagacaagc aaggcgaaag aagctgcgtg gctttcagga aagccatagg agccgaagcc
     1321 ctcgatctgt ttgaaacagc gttcggcaaa atcgcgcgag taccgacgcg caaccatgcc
     1381 ttcgaccatt tttgcgcgaa atgtctcgat gatgccgacg cgtttgaagg tcgccatggc
     1441 gcggcgcaac ctgtccgcct cggccggggt gaagcctgcg gcgacgatgg cgattttcat
     1501 cgcctgttcc tggaagagcg gaacgccaag cgtcttgctc agcacctctt tgagttctgg
     1561 tgaaggataa ttaacaagct ctggattctg ccgccgcagc agatagggat gcaccatgtc
     1621 gccctgaatg gggccgggcc ggacgatcgc gacttcaatg acgagatcgt agaaagtctg
     1681 cggccgcaga cgcggcagca tcgacatttg cgcgcggctc tcgatctgaa agacgccgat
     1741 cgtatcggcg tgcgagatca tgtcgtagac cgcgcgctct tccggcggaa tgctggcgat
     1801 cacatgatcg acgttgtaat gtgcacgcag aaggtcaaag ccctttcgta aacatgtaag
     1861 cataccgagc gcgaggacat cgaccttcaa gataccaagt gcatcgagat cgtccttgtc
     1921 ccattcgacg gttgtgcgct cgtccatcgt ggcatgggca ataggcacgg tttcatcgag
     1981 ccggtcgcgt gtgatgacga agccgccggt gtgctgcgaa agatggcgcg ggaaaccgac
     2041 aagctcttgc gaaagcgcaa gggtttgcgc taggcgatcc tcttgcggat cgaggccaag
     2101 gcggcggatg tgtttttcct gcggaccttc ggatgcgccg ccccagagtg aactcgacaa
     2161 cgcggaaatc gcatcttccg aaaggccaaa gaccttgccg acatcgcgga gcgccgagcg
     2221 actgcgatag gtgatgaccg tcgctgtgag cccggcgcgc gcacggccat agcgattgta
     2281 aatatattgc atcacctctt cgcgccgctc gtgctcgaaa tcgacatcgg tgtcgggcgg
     2341 ctcgccgcgg tcttttgaga tgaagcgttc gaagagcagc gcagtttgcg caggatcgac
     2401 atccgtgatg ccaaggcaat agcaaatcgt cgaattggcc gccgagccgc gcccctggca
     2461 gagaatgtct ttattgcgcg cataacgcac gatgtcatgc acggtgagaa agtaaggcgc
     2521 gtaattcatc tccgcgacga gcgcgagctc gtaagcgagc gcgtgtttga ctttctcagg
     2581 gaccggacct ttgtagcgtt ctgcggcgcc ggcataagta aaggcttcga gcgccgcttg
     2641 cggcgtggca tagccctcgc gcaattcttc cggatattgg ttcttgagat gggcgagcga
     2701 gaaggtaagg ccttcgagaa agcgcaaagt ctcgtccacg gcctcaggtg cttcggcgaa
     2761 aagccgcgcc atttcgcgac cgtctttcaa atgccgttcg gcattggctt gcaatttccg
     2821 gccgattgca tcgagcgtcg tgccgttgcg gatggcggtc agcacatcct gcaacggccg
     2881 ccgcgccgct tcatgcatca gcacatcgtt ggtggcgatg agcggcatgc caagacgtcg
     2941 tgccaacgcg gcgcgttcgg ccagtgcctg gcgcatctgc gggccataga tcatactcac
     3001 tgcgagccag acgcggccgg gggcggcgtc acgaagtcgc tgcaggaatg tgtcgtcagc
     3061 cccgctgggg aattcagttg cgtgaagcgg aagcctgtcc tccgcgttat ctgcgaaatc
     3121 cgcatagacc ttgccgggct ttgcggcatc gtcgtccggc tcccaagtgc ttgtcgacag
     3181 aaccgcgaat tgcaggcctt cgcttgcctc gagcagatcg gcggcttcga gccggcacag
     3241 gcctttctgt cctttcagat tgccgcgtgt gagaaggcgt gtgagccgtg cataggcggc
     3301 gcggtcttgc ggatagacaa gaatgtccgg cgtgccgtct taaaaaacca gacggcaacc
     3361 gatggcgatg cgcaaa
//
LOCUS       DASWOG010000159         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_113743,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000159 DASWOG010000000
VERSION     DASWOG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113743"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VGB93_03200"
     CDS             complement(<1..766)
                     /locus_tag="VGB93_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0145164.1"
                     /translation="MKPKILISAAFFAAFSLPAFAADLPSEKGPPAFAPPPPPVFSWT
                     GVYIGAQVGYEWGHTSPGVDTGAIAGVVGLPGFRDQGVVGGGHVGYNYEVGSLGGFLP
                     GLGGNGGGIVVGIEGDVNGASDTASCVDGTGLFFGRERTEVDASIRGRLGVAFDRVLI
                     YGTGGAAFGSIRNSVTDPFGFVEDHTTGRVGWTAGGGIEYAIDNNWSIRAEYRYTDYG
                     HFGTTYTTLFPAPFTVRYHDTDNRVQAGFSYKFDEAP"
     gene            complement(905..2038)
                     /locus_tag="VGB93_03205"
                     /pseudo
     CDS             complement(905..2038)
                     /locus_tag="VGB93_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2145..3161
                     /gene="dusA"
                     /locus_tag="VGB93_03210"
     CDS             2145..3161
                     /gene="dusA"
                     /locus_tag="VGB93_03210"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY0145165.1"
                     /translation="MVEIAEKSLNINSHRFCVAPMMDWTDRHCRVFHRLLSRRARLYT
                     EMVTAAAILHGDRQRLLGFDAQEHPLAVQLGGSEPSMLAEAAAIAADFGYDEINLNIG
                     CPSERVQNGAFGACLMREPDLVADCVAAMKARVRVPVTVKCRIGVDEQAPQALSDFAA
                     RVIAAGADLLIVHARKAWLKGLSPRENRNVPPLDYPRVRALKNEYPDVPIVLNGGLIE
                     ISQMQTELAHLDGVMVGRAAYQDPAILMTVDSALFGEPAPMPSRHAALEAFMPYIEAR
                     LADGVPLSSMTRHVLGLYNGLPGARAFRRPLATEAVKSGAGLDVLRAAMNFVPEHAIA
                     EAAE"
BASE COUNT          650 a          934 c          942 g          696 t
ORIGIN      
        1 gtggtgcttc gtcgaactta tagctgaagc cagcctgcac tcgattgtcg gtgtcatggt
       61 atcgcacggt gaagggcgcc gggaagaggg tcgtgtaggt cgtgccaaaa tggccgtaat
      121 ccgtgtagcg gtattcggcg cggatcgacc aattgttgtc aatggcatat tcaatgccgc
      181 cgccagccgt ccagccaacg cggcccgtcg tatgatcttc aacgaagccg aaggggtctg
      241 tgacgctatt gcggattgaa ccgaaggccg caccgcctgt gccgtaaatg aggacgcggt
      301 cgaaggccac gccaaggcga ccacggatcg atgcgtcaac ctcggtccgc tcgcggccga
      361 agaaaaggcc cgttccatcg acacaactag cggtatcgct tgcgccgttc acatcgcctt
      421 caataccgac aacgattccg ccaccgttgc cgccaagacc aggaaggaag ccgccaaggc
      481 tgccaacttc ataattgtag ccgacatggc cgccaccaac gacgccttgg tcgcgaaatc
      541 ccggcagtcc gacgacgcct gcaatcgctc ccgtatcgac gcctggcgac gtatgacccc
      601 attcataacc gacctgcgca ccgatgtaga cgccagtcca agaaaagacc ggcggtggcg
      661 gaggcgcgaa tgccggcgga cccttctcgc ttggaagatc ggctgcaaaa gccgggaggg
      721 agaaagcggc gaaaaatgcc gcggatatta aaattttcgg cttcattgtc agccctctta
      781 tggatcgagt cgatttgcac agactgatcg tcatccgaag gatcgtcgat gatataagtt
      841 agtcgaaagt gatttcgctt ctgactgtga ctatcggaca acgcatcaga ccgaaagcgt
      901 agctttagcg gcgcgtatta gcgctggcgc gaaggttgat attcggcgtc gtcacgaccg
      961 gtaaccattt ccatccgaca tatttatcgc cgacgtgctc gagatgagta taccgcttca
     1021 agctcgtcca tgaccgatga ccggaaacgg cggctgcgcg cgggatcgtg taatccatct
     1081 cgaacaagcg cgagatgccg tcatgtctga gatcatggaa atgcagatct tcgatgccga
     1141 gtagggtgca tgcccgcgtg aaggccgcac tgattgcgtc ggctccaaaa gggaaaatcc
     1201 tatcatctgt cttcggcatt gcccggatga tttgcagcgc ttcgggcggt agatcgcaca
     1261 agacgtcgtt gccccttttt tggccaggat ttttcatgtc tcggaccata attcggctct
     1321 tggcctcttg gaggtccaac catccgatgc gggttatctc ttcctggcgt cgggttgcga
     1381 agatcgcaaa tccgatgatg tgctacattg gtgcagagtc gggccggcgc gcagatcgat
     1441 cgccgaaatg ctccatcaac ttatcgagtt cattcaacgt cgggcgccga tcccgctgat
     1501 ctgacttttt cgcaatgctg agttttttcg cgacggtcca cgcatcttcc atagcttgag
     1561 catcgagcgg gtatccccac gccggccgcg cgatcgtgaa cacggcactg agatgtgaca
     1621 ggtagttgcc gacggtctga ggggtcacgc caatggcgag cttctgcctg gcaaatttga
     1681 tgatttcgac gctagtgacc tagtcgcagg cgagatcagc gatctcgaat tcctttatcg
     1741 acttcagaac ctgcgctttg gtgcgaccga tctctttctc gctttccgcg accatgcgat
     1801 cgattgcgac tgccagggtc gcggagcggg agccgtgctt cttggctgtg attgcctctt
     1861 cgagtgcttc cggcttttca agctccttct cgcgcttctt gatccaggca ttggcggcct
     1921 gcgggcggtc gaaggtcttg gtctctcgca aaacgatttt acggtcgcgc ataatcgaga
     1981 tttgcgcgtg atgggctata gtgccacccc gacggcggcg cgttacgacc gttcccattt
     2041 acagcacgcc ctcaggattt acagctcgtg ctgtagaaat aggacgaaat gggccgaaaa
     2101 ggcaataaac gcgattaaat cgggcagaca aggacagagc ttaattggtt gaaatcgcag
     2161 aaaaatcctt aaatatcaac agccatcgtt tctgcgtggc cccgatgatg gattggacgg
     2221 accggcattg ccgtgttttc catcgtctat tgtcacgccg cgcgcggctt tacacagaga
     2281 tggtgacggc cgcggcgatc ctgcatggcg accgccagcg actgcttggc ttcgatgcgc
     2341 aggaacatcc gcttgccgtg cagcttggcg gttcggaacc gtcgatgctc gccgaagctg
     2401 ccgcgatcgc ggccgatttc ggctatgacg aaatcaatct caatatcggc tgtccatcgg
     2461 agcgcgtaca gaacggcgcg tttggagctt gcctgatgcg cgagcccgac ctcgttgccg
     2521 attgcgtcgc ggcgatgaaa gcgcgcgttc gtgtgcccgt caccgtcaaa tgccgcatcg
     2581 gcgtggacga acaggcgccc caggctttgt cggacttcgc cgcgcgggtg atcgcggccg
     2641 gtgctgatct gctcatcgtt cacgcccgca aggcctggct gaaaggtttg tcaccgcgcg
     2701 aaaatcgcaa cgtcccgccg ctggattatc cgcgcgtgcg cgccttgaag aacgaatatc
     2761 cggacgtccc gatagtgttg aacggcggct tgatcgagat cagccagatg cagaccgaac
     2821 tcgcgcatct tgatggcgtg atggtgggcc gcgccgcgta tcaggacccg gcgattctca
     2881 tgacggtcga ttccgctctc ttcggcgaac ctgcacctat gccaagccgc catgccgcgc
     2941 tcgaagcctt catgccctat atcgaagcgc gccttgccga tggcgtgccg ctctcttcga
     3001 tgacacggca cgttctcggg ctttacaacg gcctgccggg cgcacgcgcc ttccgccgcc
     3061 ctctcgccac agaggcagtg aaatctggtg cgggactaga cgtgctgcgc gcggcgatga
     3121 acttcgtgcc ggagcatgcg attgcggagg ccgccgaatg aatagcggag acatgcgcga
     3181 cgctgtggcg agcaaggtgc ccactgtaac gcttatcttc tc
//
LOCUS       DASWOG010000160         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_3802, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000160 DASWOG010000000
VERSION     DASWOG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1220
                     /locus_tag="VGB93_03215"
     CDS             96..1220
                     /locus_tag="VGB93_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0145166.1"
                     /translation="MSTPALVIGGGLAGAGIAVSLANAGRAVTLVERETRAQHNVCGE
                     FISAEAMLYLRDLGIDLDALGAVSIDRVGIFAGERQATAYLPFSARSLSRYALDEALL
                     NRAARCGARVLRGRKALGVERDEHRWRASLDDGTSVSASEIFLATGKHDLRGWKRPPG
                     SQNDLIGFKLHWRLAVSQMQSLRGAVELFLFPGGYAGLEGVENDVANLCLVVRRQTFA
                     CGQSWPTLFASLLKSCPHLRRRLDGAQPCWPRPLAISAIPYGFVQRRTDGLWRLGDQA
                     ACTPSFSGDGMSIALHSARLAAQYFSQGHDARSFQARLAQDVAPQVQRATLLSQMLLQ
                     PLGQKAGMAALERLPLLLRVVARATRLPQNRVDEARAGPV"
     gene            1517..>1759
                     /locus_tag="VGB93_03220"
     CDS             1517..>1759
                     /locus_tag="VGB93_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145167.1"
                     /translation="MARYYGELYETISRLSQIYIARPARHFPETRPPRQHKIGGRTFI
                     VAPIFLGALIGSTFEFNAARAQQASTGGTEALPQIDV"
BASE COUNT          338 a          537 c          505 g          379 t
ORIGIN      
        1 gattggcaac atcttcttgg cgccgccgat atctcgggcg cccgcgtcaa agcctatatg
       61 ccattccggc tctgcgtatc gcaaatcaag ccgaaatgag cactcccgcc ctggtcatcg
      121 gcggcggcct cgcgggcgcg ggaattgcgg taagcctcgc caacgccgga cgcgccgtta
      181 ccctcgtcga gcgtgaaacg cgggcacaac acaatgtctg tggcgagttc atcagcgccg
      241 aagccatgct ctatctgcgc gatcttggca tcgatctcga cgccttgggc gcggtttcaa
      301 ttgatcgcgt tggcatattc gcgggcgaga ggcaggccac ggcatatctg ccgttttcgg
      361 cgcgaagcct ttcgcgctat gccctggacg aggctctgct caacagagcg gcgcgttgcg
      421 gcgcccgggt gctgcgtgga cgcaaggcac tcggcgttga gcgagatgaa catcgttggc
      481 gtgcctcgct cgatgatgga acgagcgttt cagcctccga aatattcctt gcaacgggca
      541 agcatgatct gcgcggttgg aagcggccgc ccggatcgca aaacgatctc attggattca
      601 aattgcactg gcggcttgcc gtctcgcaga tgcagtccct gcggggcgcg gtcgaactgt
      661 ttctttttcc aggcggatat gccggacttg aaggggtcga gaacgatgtc gccaatcttt
      721 gccttgtggt tcgcagacaa acgtttgcct gcggacaaag ctggccaacc ctgttcgcgt
      781 cgctgctgaa aagctgcccg catctgcgac ggcgtctcga tggcgcacag ccctgctggc
      841 cacgaccatt ggcgatcagc gccatcccct atggcttcgt gcagcgtcgc acggatgggc
      901 tctggcggct tggcgaccag gcagcctgca cgccgtcatt ttccggcgac ggcatgtcga
      961 tcgcgctcca ttcagcgcgg cttgccgcgc aatatttttc gcaaggccat gacgcacgaa
     1021 gcttccaggc gcgccttgcg caagacgtcg cgccacaggt gcagcgcgca accttgcttt
     1081 cgcaaatgct gttgcagccg ctcggccaaa aagccggcat ggcagccctc gaacggttgc
     1141 cattattgct gcgcgtcgtt gctcgcgcga cgcgtcttcc gcaaaaccgt gttgacgagg
     1201 cgcgcgctgg ccctgtctga gcccgcgtgt taccgctgcg caacggcgca gacgtttatt
     1261 tcaaagtcac atcgcagaac tcgattctcc gggcgtgccg aatccacgct caaattcttg
     1321 cttgcctgct gattaagcaa agcttccgtg caagatttaa gtcattcaaa acctgacatg
     1381 aaagtcagtc ttataagtat tacagactac ttcgattgac cgttcatgta tttcaaaatt
     1441 atcagcccaa tgctcaccac gccgtggcgt tggtgctcga attttttgga agggctgcaa
     1501 ggacatgacc tgtgtattgg cgcggtatta cggcgaactt tacgagacta tctcgagact
     1561 atctcagata tacattgcgc ggccggcccg tcactttcct gaaacgcgtc ctccgcggca
     1621 acataagata ggtggacgca cgttcatcgt cgccccgatt ttcctcggcg cgctgatcgg
     1681 atcgacattc gaattcaacg ccgcccgcgc gcaacaggcg agcaccggcg ggacggaagc
     1741 cctaccccag atcgacgtg
//
LOCUS       DASWOG010000161         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_40104,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000161 DASWOG010000000
VERSION     DASWOG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40104"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VGB93_03225"
     CDS             complement(<1..702)
                     /locus_tag="VGB93_03225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0145168.1"
                     /translation="MTVKNAHLRAAQSEGAATDARADLASRVARVVEIAATHAAAVDA
                     EARFPTAAIEAMREGGLLSLMVPHDLGGEGLSLGAIADVCYGLGQACSATAMIFAMHH
                     SAAACVIRHTGASAWHRDFLRQIANAQLLLASSTTEGQNGGNVRASAAAIEGSGDSIT
                     LDRAATVISYGAEADVIVTTARRADDAPSSDQVLVTFVKGDYTLEPLSGWNALGMRGT
                     ASAGFTFKRAVTARKF"
     gene            946..1218
                     /locus_tag="VGB93_03230"
     CDS             946..1218
                     /locus_tag="VGB93_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0145169.1"
                     /translation="MPGQATPREKLIDLVAAILKRNGVTRPVDADADLVNQGLTSVDM
                     VHLMLAIEAAFDITIPQSGLTPENFRSIATIEALLAGLAAPAEASP"
     gene            complement(1223..>2282)
                     /locus_tag="VGB93_03235"
     CDS             complement(1223..>2282)
                     /locus_tag="VGB93_03235"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0145170.1"
                     /translation="PARAPNVTNLVLKEPVGPVAAFAPWNFPLNQIVRKLGAALAAGC
                     SIIVKAPEETPASPAELIRAVVDAGVPAGVVNLVYGVPAEISEHLIPHDVIRKISFTG
                     STAVGKQLAALAGKHMKRITMELGGHAPAIVFDDADVDAAVKLLVTSKYRNGGQVCTA
                     PTRFLVQRKVYGPFLDRFVAAAESLKVGDGTAPDTQLGPLANPRRIDAMEALIADAVG
                     KGAELRIGGTRLGNKGYFFAATVLANVPLAARAMNDEPFGPLALVNAFDDLDEALAEG
                     NRLPYALASYAFTSSAMTSAALAARLEAGMLGINQTQVAWPELPFGGVKDSGYGREGG
                     SEAIQDYLVTKLVTQAAG"
BASE COUNT          470 a          717 c          644 g          451 t
ORIGIN      
        1 gaatttgcgc gcggtcaccg cgcgcttgaa cgtgaagccg gcgctggccg tcccgcgcat
       61 tcccagcgca ttccagccgg acagcggctc cagcgtgtaa tcgcccttaa caaatgtcac
      121 gagaacctga tccgagcttg gcgcatcgtc ggcgcgccgc gctgtcgtca cgatcacatc
      181 ggcttcggcg ccgtaggaaa tgacggtggc ggcgcgatcg agcgtgatcg aatctccgct
      241 cccttcaatt gctgcagcac tggcgcggac attgcccccg ttttggcctt ccgtcgtcga
      301 agatgcgagg agcaattgcg cgttggcgat ctgccgtaga aagtccctgt gccaggcgct
      361 ggcgccagtg tggcggatca cacaagcggc ggcgctatga tgcatggcaa agatcatcgc
      421 ggtggcagaa catgcctgtc cgaggccata acagacatcg gcgatcgcgc ccagcgaaag
      481 accctcgccg ccgagatcgt gcggcaccat caggctcagc aaaccgccct cgcgcatagc
      541 ctcaatcgcg gcggttggaa aacgcgcttc cgcatcgacg gccgcggcgt gcgtggctgc
      601 aatttcgaca acacgcgcga cacgcgatgc cagatcggcg cgagcatccg tcgccgctcc
      661 ctcgctttgc gccgcgcgca gatgagcatt tttcactgtc atccagaaat ccagtccacc
      721 tgcggccgca cacgaataag agacgtaatt cgtctcattt agccgaaata ctcaactcag
      781 ccattaattc cgctcttcac gaatttctgt tacttgagcc ttagttaact aaccctccgg
      841 caattgctgg accaattgta accattcatg gttaatgccc aggattttat ttgtatctgc
      901 gtttttcttc gctaaaagtt cgagataatt tccacggaga ctttaatgcc aggacaggcc
      961 acaccgcgag agaagttgat agacctcgtc gccgccattt tgaagagaaa cggcgtcacg
     1021 cggccagttg acgcggacgc cgaccttgta aatcagggcc tcacctccgt cgacatggtg
     1081 catctaatgc tcgccatcga agccgccttt gatatcacca ttccgcaatc ggggctgacg
     1141 ccggaaaatt tccgctcgat tgcgaccatc gaagctctgc tcgccgggct tgccgccccc
     1201 gcagaagctt cgccctaaac atttagcccg ccgcctgcgt gacgagcttc gtcacgagat
     1261 aatcctggat ggcttcgctg ccgccctcac ggccataacc actgtccttg acaccgccga
     1321 aaggcaattc tggccaagca acctgcgtct ggttaatgcc gagcatgccg gcctcaagac
     1381 gcgccgccaa agccgcgctc gtcatggctg aacttgtgaa ggcataggag gcaagcgcat
     1441 agggcagccg attgccctcc gctagagcct cgtcgagatc atcgaatgca ttgacgagcg
     1501 caagcggccc gaagggctcg tcgttcatcg cgcgcgccgc caatggcaca tttgccagca
     1561 cagttgctgc gaagaaatat cctttgttcc caaggcgcgt gccgccgatg cgcagctccg
     1621 cgcccttgcc gaccgcatcg gcaataagtg cctccattgc atcgatgcgg cggggattgg
     1681 ccaagggacc gagttgcgtg tctggtgccg tgccatcgcc gactttcaac gattccgctg
     1741 ccgcgacaaa gcgatccaaa aatggtccat agacttttcg ctgtacgaga aagcgcgtcg
     1801 gcgcggtgca gacctggccg ccgttgcgat atttcgaagt gacgagcagc ttgactgccg
     1861 catcgacatc cgcgtcgtca aagacgatgg cgggtgcatg gccgccgagt tccatcgtga
     1921 tgcgtttcat atgcttgccg gccagcgccg caagctgttt gcccacggca gttgagccgg
     1981 tgaacgatat tttccggatc acgtcatgcg ggatgagatg ttcggagatt tccgccggca
     2041 caccgtaaac gagattgacg acgccagccg gcacgcccgc atcgacgacg gcgcggatca
     2101 attccgccgg cgaggccggc gtctcttccg gcgctttcac aatgatcgaa cagccagcag
     2161 caagcgccgc accgagtttg cggacaatct gattgagcgg aaaattccat ggtgcaaagg
     2221 cggcgaccgg gccgaccggc tctttcaaga cgagattggt gacattcggc gcccgcgccg
     2281 ga
//
LOCUS       DASWOG010000162         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_333565,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000162 DASWOG010000000
VERSION     DASWOG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333565"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..573
                     /locus_tag="VGB93_03240"
                     /pseudo
     CDS             1..573
                     /locus_tag="VGB93_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965119.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(574..1377)
                     /locus_tag="VGB93_03245"
                     /pseudo
     CDS             complement(574..1377)
                     /locus_tag="VGB93_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456393.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            1556..1846
                     /gene="rpmB"
                     /locus_tag="VGB93_03250"
     CDS             1556..1846
                     /gene="rpmB"
                     /locus_tag="VGB93_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0145171.1"
                     /translation="MSRRCELTGKAVLVGNLVSHSNRKTKTRFLPNLVRVTLTSESLG
                     RSVRLRVAAAALRSVEHRGGLDAFLTKASETQLSTGARALKREIEKKQQASA"
     gene            complement(1892..3823)
                     /gene="cobT"
                     /locus_tag="VGB93_03255"
     CDS             complement(1892..3823)
                     /gene="cobT"
                     /locus_tag="VGB93_03255"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEY0145172.1"
                     /translation="MAGSNHKPPGKAEAPQEPFKRAVANCLKAMANLPQLDVTYAAEK
                     PNLAGANAGARVRLPEPPRKLTRQDAAILRGNADAMALRLACHDSNVHRRLLPQTPQA
                     RAVFEAVEQARVEAIGARRMEGVAQNLGAMLEDRFQHARFGEIRDRADAPLEQAVAMM
                     VRERLTGQKPPKSAQRLVDLWRPVIEQRAGRDLDQLSASIEDQRTFGHSVRRVLSALD
                     MGEDLGSDTNNEETEQSDDQQAGGDDNEAFEGQRDESADSAQSQTTETAEADGETEAD
                     SIDAASGEFEAEEEGDLGEGDQAADAHRPPPKARTEVRAPDYQAFSRKFDETIAAEAL
                     CEPEELERLRAYLDKQLQNLSSVVGRLANRLQRRLLAQQNRSWEFDLEEGMLDSARLS
                     RIVIDPQQPLSFKREKDMDFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCG
                     VKVEILGFTTRAWKGGQSREAWLQAGKPPSPGRLNDLRHIIYKAADAPWRRARKNLGL
                     MMREGLLKENIDGEALDWAHKRLLARPEQRRILMMISDGAPVDDSTLSVNPGNYLERH
                     LRYIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAMTEKLAELFDEKAE
                     PEQPQHRARTKRMPVVTVH"
     gene            complement(3825..>4463)
                     /locus_tag="VGB93_03260"
     CDS             complement(3825..>4463)
                     /locus_tag="VGB93_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY0145173.1"
                     /translation="KQVTEFREGMLPWALQRPIALVFDEYDAGRPDVMFVIQRVLEVS
                     GRLTLLDQNRVIKPHPAFRLFSTTNTIGLGDTSGLYHGTQQINQGQMDRWSIVATLNY
                     LPHDKEVGIVLSKVKAYQKTKETRDQVGKMVRVADLTRNAFMAGDLSTVMSPRTVITW
                     AENAEIFKDIGFAFRLTFLNKCDELERALVAEFYQRCFGQDLTESAANVAMS"
BASE COUNT          811 a         1385 c         1403 g          864 t
ORIGIN      
        1 gtgccgatcg gcgcggtgct catgcgccgc aagattttcg agagcgtctt caaccggatg
       61 gaccgcatgg tcgtgcatgg ctcgaccttc gcgaaaaacg atctggcgat ggcggcaggc
      121 ctggccacgc tggaagtgat cgagtccgag cgcctgatcc agaacgccgc ccggcgcggc
      181 gagcagcttc ttgccttctt caagcctctc gccgagcgtt acgaactcgt caagagcgtc
      241 cgcggcaagg ggttgatgat cggcatcgaa ttcggccagc cgcgttcctt caagcttaag
      301 gccgcctgga cgttgctgga atcagtgcgg gcggggctgt tctgccagct catcgttatt
      361 ccgctgttca aggatcacaa agttttggtg caggtggcag cgaacgccgg ccatacgata
      421 aagcttttgc cgacgctcaa catcaatgac gcggattgca cctagatcga gggcgccttt
      481 gacagcgtgg tggccgatgc acatcgcgtc cccggtgcgg gctggtcgct cggcaaaacg
      541 ctcgccgaaa acgtccgcaa aggcagccgc tgatcatccg tcacgacgtt gagtggcaac
      601 gaaatcaacg aaggcgcgca gcggcgccgg cagatagcgg cgcccgggat agtaaagaaa
      661 cgggccggaa aagcgctgcc accacggttc cagcaccggt tcgaggctgc cgttcttgaa
      721 atagggtagc agccaatccg cgaagagccc gacaatgccg ctccccgcga tggcagcctc
      781 aacgaccaga tcgctcgcgg tgccagcgct gacaacgagc gggcctggcg ggtcgatctt
      841 cacgacctct ccctcacgct caaactccca gaccggaatt gcaccgcttg gaaagcgtat
      901 acgcagacac gcgtgcgaaa gtagatcgcg ggggtgctct gggcgtccgt attcttcgaa
      961 ataggaaggc gacgcggcca cagcaaagcg ttgctcgggg gggggccaat ggggacagcg
     1021 atcatatcct gctccaggcc tcgtatcgaa tccccgcatc gcatccggcg gaaagaagat
     1081 cgacgaagct ttcttcggct ataacttcca ccaaaatatc ggggtaggcg gcgataaatc
     1141 ggggcacgat gcttggcagg accagtcgcg cagcgctcgc ggggacgttc aaccgaagtg
     1201 tgcctgctgg ccggtcgcgg aagaagttga gcacgtccaa tgcagactcg acttcgctga
     1261 gtgccggacg gagccgatcc agaaggcgcc cgcccgcctc tgtgggtgcg acgctcgtgt
     1321 ggtgcgattg agcaaacgga cgttgaggca cttggcggca acggacctga agctctccag
     1381 cgaagcaatc gccgcactgg atcgtattgc ggatggaggt tgagaaaagc caacgggtca
     1441 ggcctcttct cttgaccccg gctgccgctt cccctaaaca gcgcgcttcg atttccgcct
     1501 gtgctcctgt gcgcgggctc tgtgtttatg gccgcaaggc cggaaggatc atcccatgtc
     1561 gcgccgctgc gaattgaccg gcaaggccgt gctagtcggc aacctggtca gccattcaaa
     1621 ccgcaaaacc aagacacggt ttttgcccaa tctcgtccgg gtgacgctca cctctgaatc
     1681 gctcggccgc agcgtacgtt tgcgcgtcgc cgcggccgcg ctgcgctcgg tggagcaccg
     1741 cggtggcctt gatgcgttcc tgaccaaggc gtccgagacc cagctttcga ccggcgcccg
     1801 cgctctgaag cgtgaaatcg agaagaagca gcaggcctcg gcgtaaaccg accgtcatgc
     1861 gcggacttga tccgcgcatg acgcatacgc ttcaatgcac ggtcacgacc ggcatgcgtt
     1921 tcgtccgcgc acgatgttgc ggctgctccg gttcagcctt ctcgtcgaac agctcagcga
     1981 gcttctccgt catcgcgccg ccgagctctt ccgcatcgac gattgtcacc gcgcggcgat
     2041 aataacgggt cacatcatgg ccgatgccga tggcgatcaa ttcgaccggc gagcgcgttt
     2101 cgatctcctc gatgatgtag cgcagatgcc gttcgagata attgccgggg ttgaccgaca
     2161 gcgttgaatc gtcgaccggc gcgccgtccg aaatcatcat caggatgcgg cgctgttcgg
     2221 gccgcgcaag caggcgttta tgcgcccaat cgagcgcctc gccgtcgata ttttccttga
     2281 gtagaccttc acgcatcatc aggccgagat tcttgcgagc cctccgccag ggcgcgtcgg
     2341 cggctttgta gatgatgtgg cgtagatcgt tgaggcggcc gggcgacggc ggcttgcctg
     2401 cctgcagcca ggcctcgcgc gattggccgc ccttccaggc gcgggtggta aaacccagaa
     2461 tctcgacctt gacgccgcaa cgctccagtg tgcgggcgag aatatcggcg catgtcgctg
     2521 caaccgtgat cggacgcccg cgcatcgagc ccgaattgtc gagcagaagt gtcacgactg
     2581 tatcgcggaa atccatatcc ttttcgcgct tgaaggacag tggctgctgc ggatcgatga
     2641 cgatgcgtga gagccgggcc gagtcgagca taccttcttc gaggtcgaac tcccacgagc
     2701 ggttctgctg ggcgagcagc cggcgctgca accgattggc gagtcggccg acgacggagg
     2761 acaaattctg caattgcttg tcgagatagg cgcgcaaccg ctccagttct tcgggctcgc
     2821 acaaagcttc ggccgcgatg gtctcgtcga acttccggga gaaagcctga taatccggcg
     2881 cccgcacttc ggtccgcgct ttcggcggcg gacggtgcgc atcggcagcc tgatcacctt
     2941 cgccaagatc gccttcttct tcggcctcga actcgcccga ggcggcatcg atagaatcgg
     3001 cctcggtttc gccgtcggct tcagcggttt cggttgtctg cgattgggcg ctgtcggcgc
     3061 tctcgtcgcg ctggccttcg aaggcttcgt tatcgtcgcc gcccgcctgc tgatcgtcgc
     3121 tctgctcggt ttcctcattg ttggtgtcgg agccgagatc ctcgcccata tcgagcgcgg
     3181 acaaaacgcg ccggaccgaa tgcccgaaag tacgctggtc ctcgatcgag gcagaaagct
     3241 ggtcaaggtc gcgccccgcg cgctgttcga tgacagggcg ccacaaatcg acgaggcgct
     3301 gcgcgctttt cggcggcttt tggccggtga gccgttcgcg caccatcatt gccaccgcct
     3361 gttcgagcgg tgcgtcggcg cgatcgcgga tttcgccgaa gcgcgcatgc tggaagcgat
     3421 cctccagcat ggcgccgaga ttttgcgcca cgccttccat gcgccgggcg ccgatggcct
     3481 cgacgcgcgc ctgttcgacc gcctcgaaga cagcgcgcgc ctgcggcgtc tgcggcagca
     3541 aacggcgatg cacgttggaa tcgtggcagg caagccgcag cgccatcgca tccgcattgc
     3601 cgcgcaggat ggccgcgtcc tggcgggtta gcttgcgggg cggctccggc aggcgaaccc
     3661 gtgccccggc attggcaccg gcgagattgg gcttctccgc ggcataagtc acatcaagct
     3721 gcggcaggtt cgccatcgcc ttcaggcaat tggccactgc ccgcttgaag ggctcctgcg
     3781 gcgcctcggc cttgcccggc ggcttatggt tcgacccggc catgtcagct catcgcgaca
     3841 ttcgccgcgc tttccgtcaa gtcctgcccg aaacaacgct gatagaattc ggcgaccagc
     3901 gcgcgctcga gttcgtcgca tttgttgagg aatgtcagcc ggaaggcgaa gccgatgtcc
     3961 ttgaaaattt cggcgttttc agcccaggtg atgaccgtac gtggactcat gacggtggag
     4021 agatcgccgg ccatgaacgc gttgcgggtc aaatcggcga cgcgcaccat tttgccgacc
     4081 tggtcgcggg tctccttcgt cttctgatag gctttcacct tggaaaggac gatgccgact
     4141 tctttgtcat gtggcagata attcagcgtt gcgacgatcg accagcggtc catctgtcct
     4201 tgattgatct gctgcgtgcc atgataaagg ccggatgtat cgccgaggcc gatcgtgttc
     4261 gttgtcgaga ataaccggaa ggccgggtgc ggcttgatca cgcggttctg atcgagcagt
     4321 gtcagccggc cggagacttc gagcacacgc tggatcacga acatcacgtc cggacggccg
     4381 gcgtcgtatt cgtcgaacac cagggcgatc ggccgctgca gggcccaggg cagcatgcct
     4441 tcgcggaatt cggtgacctg ctt
//
LOCUS       DASWOG010000163         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_58638,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000163 DASWOG010000000
VERSION     DASWOG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58638"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGB93_03265"
     CDS             complement(<1..231)
                     /locus_tag="VGB93_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0145174.1"
                     /translation="MNFQAIRAIYVYEMSRTRRTLLQSIVSPILSTALYFVVFGSAIG
                     SRISHVGGVSYGAFIVPGLIMLNLLTQSISNAS"
     gene            complement(228..1154)
                     /locus_tag="VGB93_03270"
     CDS             complement(228..1154)
                     /locus_tag="VGB93_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145175.1"
                     /translation="MSPIISVSHLSKTYSGGRAALKDVSLEIGKGEIFALLGPNGAGK
                     TTLISIICGIVTPTAGTITAAGHDIIRDFRAARAMIGLVPQELTIDFFDSVWATVKLS
                     RGLFGKAPDDAYLESLLRDLSLWDRKDSQARTLSGGMKRRMMIAKALSHQPDILFLDE
                     PTAGVDVELRRGMWDLVRKLRAAGTTIILTTHYLEEAEEMADRVGVIRQGELILVEDK
                     NRLMLKLGKKKLTLMLAAPLAALPASLQAYPLSLSDDGRELTYTYGEDGGAAPVAALF
                     DSLRTAGVEVSDLRTEQSSLEDIFVSLIGKTA"
     gene            complement(1239..>2078)
                     /locus_tag="VGB93_03275"
     CDS             complement(1239..>2078)
                     /locus_tag="VGB93_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145176.1"
                     /translation="STGTLIFLPGLAGLAAWGGWKPVVLTIAGGALALIPLIAFLVPE
                     SPAAIGLRRYGATEDEPAAPAPAHDPFTVALGDLMVAARMRTFWFLFATFFICGFTTN
                     GLVGTHLIAFCGDNGIAEVHAAGLLAMMGIFDIFGTTFSGWLTDRYDPRKLLFVYYGL
                     RGLSLIYLPYSNFSFVSLSLFAAFYGLDWIATVPPTVRIANEKFGDKNAPIIFGWVVA
                     GHQLGAATAALLAGLIRTAQGSYLEAFVIAGATGLAAAFLSLMIEKQKVAPQFAGALE
                     AGE"
BASE COUNT          433 a          619 c          676 g          350 t
ORIGIN      
        1 cgaggcgttg ctgatgctct gcgtcaacag gttgagcatg atcagccccg gcacgatgaa
       61 ggcaccgtag cttaccccgc cgacatgcga gatacgcgag ccgatcgccg agccgaagac
      121 cacgaaatag agcgcggtcg acaggatcgg cgagacgatg ctctgcagca gggtgcgccg
      181 cgtgcgcgac atctcgtaga cgtagatcgc ccggatcgcc tggaaattca tgctgtcttt
      241 ccgatgaggc tgacgaaaat gtcttcgagc gaagattgct cggtgcgcag atcgctgact
      301 tcgacgccag ccgtgcgcaa actgtcaaac agtgccgcga caggcgcggc gccgccgtct
      361 tcgccatagg tataagtcaa ctcgcggccg tcgtccgaaa ggctcagcgg ataggcttgc
      421 agcgaagcag gcagggcggc gagcggggcg gcgagcatga gcgtcaactt cttcttgccg
      481 agcttcagca tcagccgatt tttgtcttcg acgaggatca actcgccctg ccggatgacg
      541 ccgacgcgat cggccatctc ctcggcttct tcgagataat gcgtggtgag gatgatcgtc
      601 gtgccggcag cgcgcagttt gcgcaccaga tcccacatgc cacggcgcaa ttcgacatcg
      661 acgccggctg tcggttcgtc gaggaaaagg atatcgggct gatgcgacaa agccttggcg
      721 atcatcatgc gccgcttcat gccgccggag agggtgcgcg cctggctatc cttgcggtcc
      781 cagagcgaga gatcgcgcag cagactttct agataggcgt cgtccggcgc cttgccgaag
      841 aggccgcgac tgagcttgac cgtcgcccac acgctatcaa agaaatcgat ggtcaattcc
      901 tgcggcacaa ggccgatcat cgcgcgggcg gcacggaaat cgcgaatgat gtcgtgaccc
      961 gccgcggtga tcgtgccggc ggtgggcgtg acgatgccgc agatgatgct gatcagggtc
     1021 gtcttgcccg cgccgttggg gccgagcagg gcgaaaatct cgcccttgcc gatctcaagg
     1081 ctcacgtcct tcagcgccgc gcggccgccg gaataggttt tcgaaagatg gctgacggag
     1141 atgatgggcg acatgggccg gatggatggc gggtgggcga ctcgggggcc gcttttagag
     1201 catcgccccc ccaagtggaa atcggatttg cgtccggcct attcgcccgc ctccaaagcg
     1261 ccggcaaact gcggcgcaac cttttgtttt tcgatcatga gtgacagaaa tgccgcggcg
     1321 aggccggttg cgccggcgat gacgaacgcc tctagatagc tgccttgcgc ggtacggata
     1381 agccccgcga gcagagccgc ggtcgccgcg ccaagctgat ggccggcgac aacccagccg
     1441 aagatgatcg gcgcgttctt gtcgccgaac ttttcattgg cgatgcgcac ggttgggggc
     1501 accgtcgcga tccaatcgag gccataaaaa gccgcgaaca gcgacaggct cacgaacgag
     1561 aagttcgaat agggcagata gatcagcgat agaccccgca gcccgtaata aacgaacagc
     1621 agtttgcgcg gatcgtagcg atcggtcagc cagcctgaaa acgtcgtgcc gaaaatatcg
     1681 aaaatgccca tcatcgccag caggccggcg gcgtggactt cggcgatgcc gttgtcgccg
     1741 caaaaggcga tgagatgcgt gccgacgagt ccgttggtcg tgaagccgca gatgaagaat
     1801 gtcgcgaaca gaaaccagaa agttcgcatc cgtgcggcaa ccatcagatc gccgagcgcg
     1861 acagtgaaag gatcatgcgc aggggccggt gcggctggct cgtcctccgt tgccccataa
     1921 cgccgcaaac cgatcgccgc cgggctttcc ggaacgagga atgcgatcaa cggaatgagc
     1981 gcgagggcgc cgccggcgat cgtcaggacc accggcttcc agccgcccca agcggcgagg
     2041 cccgcgaggc cgggcagaaa gatgagcgtt ccggtcga
//
LOCUS       DASWOG010000164         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_333591,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000164 DASWOG010000000
VERSION     DASWOG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333591"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGB93_03280"
     CDS             <1..319
                     /locus_tag="VGB93_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145177.1"
                     /translation="TGGLSYATVDNYYNVAGVSISRTQTHAGVFPNFGVVGIGAEYAI
                     TDHFSVQAEYLYDFNGARWDQLNSPATGIVGFGTRAMYHIARLGLNYKFESFGGAPIE
                     SKY"
     gene            412..1667
                     /gene="ligA"
                     /locus_tag="VGB93_03285"
                     /pseudo
     CDS             412..1667
                     /gene="ligA"
                     /locus_tag="VGB93_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167911.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
BASE COUNT          341 a          474 c          478 g          374 t
ORIGIN      
        1 gaccggcggt ctcagctacg cgaccgttga taattattac aacgttgctg gcgtctccat
       61 ttcccggacg caaacacacg ccggtgtatt tccaaatttt ggtgttgtcg gcatcggcgc
      121 tgaatatgcg atcaccgacc atttttcggt gcaagcggag tatctgtacg atttcaacgg
      181 cgcacgctgg gatcagctta atagtcccgc cacgggtatc gtcggtttcg gcacgcgcgc
      241 gatgtatcac atcgcgcgtc tcggcttgaa ttataaattt gagtcctttg gcggtgcccc
      301 aatcgaatca aaatattgag tgaggcggac acgaccaagc ttatcgtcga attcgccgca
      361 ccagcgtgaa atcggtttcc tctttcggtc gcgatgcgct aaattttcga cgtgacaaag
      421 ccaaagactc tcctcgctgc tgacagcgcc gacgcccgca cggaatatgt tcgcctcggt
      481 gccgaaatcg cgcatcatga cgcgctttat tatcgcgagg atgcgcccga aatttctgac
      541 gcggactatg acgcgttgcg ccgacgctat gaggcgctgg aaaaggctca tcccgaattc
      601 gtcaccgaca aatcgctgag ccaacgcgtt ggcgcggcgc cgtcagaaaa attcgtcaaa
      661 gtccgccaca aagtgccggt gctctcgctc ggaaatgttt tcgccgattc cgatgtcgaa
      721 gaattcgtcg cacgcgtccg gcgtttcctt ggacttgccg ctggctcgcc gctggcgatg
      781 acggctgagc ccaagattga cggcctgtcc tgttcgctgc gctatgagcg cggccggctt
      841 gtcctcgcgg caacacgcgg cgatggctat gagggcgagg acgtcacggc aaacgtcaaa
      901 actatccacg agataccgca tatcttgaag cacgatccgc cggacgtgct tgaggtgcgc
      961 ggcgaaatct atatgacgcg cgttgatttc accgcgctta acgcgcgcca ggcggaggcc
     1021 ggcaaggcgc tcttcgccaa tccgcgcaat tccgcagcgg gctcgttgcg gcagcttgat
     1081 cccgcgatca ccgcatcgcg gccgttgcac ttctttgctt atgcctgggg tgaggtgagc
     1141 tagatgccgt caacgaccca gatggggatg gtcgggaaat tcgatcgata tgggttcacg
     1201 gtcgatccac tgacaatgct ttgccattca gcagcagact tgttgcgcca ttatcatgac
     1261 atcgaaagcg gccgggcttc gcttggctac gacatcgacg gtgtcgtcta taaggtcgat
     1321 gatcttgcct tgcaggaacg gctgagcttc gtctcgcgcg cgccgcgttg ggctgttgcg
     1381 cataaattcc cggcggacca ggcgacgaca atccttcgtg atatcgaaat tctggtcggc
     1441 agcactggcg ccatgacacc tgttgcgcgg ctcgatccgg tcaatgtcgg tggcgtcatc
     1501 gtcgccaatg cgacattgca ttatgaagat gagatcgcgc gcaaagacat tcgcatcggc
     1561 gataccgtac ggttgcagcg cgctggggat gtcatcaccc aaatgatcgg cgttgttctg
     1621 gaaaagcggc cgacaggttc agagccatat aagtttccgc acaaatg
//
LOCUS       DASWOG010000165         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_223797,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000165 DASWOG010000000
VERSION     DASWOG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223797"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGB93_03290"
     CDS             <1..527
                     /locus_tag="VGB93_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0145178.1"
                     /translation="ADGKAATDFPIYKNAAALSAVTALYKHVPVLDTGGRGATVTFAQ
                     KQIGDVLVNWENELYLASDEFGKDKFEIVYPSTSVLAEPPVAVVDSIVDKKGTRAVAT
                     AYLGYLYTPEAQDAIAQYHYRPRNIDIYKKYEATLPPVKLFTVDQAFGGWPRAQKTFF
                     ADGGVFDQIYKPTK"
     gene            650..1555
                     /locus_tag="VGB93_03295"
     CDS             650..1555
                     /locus_tag="VGB93_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0145179.1"
                     /translation="MAGARKVIPGFRLTLGYTLFYLSLLVLIPLAALVLKTTQLSWSE
                     FVATITDPVVVAAYQLTFGAAALAAVVNGLFGLIVAWVLVREPFPGRRYFDALIDFPF
                     ALPTAVAGITFSNLYLTDGWIGGFGDHAVAFLNGLAGHFGHPNALSAGALSWLDFPYS
                     NTNTGIVIVLIFVGLPFVVRSVQPVLQEWDPQYEQAAYSLGASGATTFRRVIFPEIFP
                     AWLSGVALAFARAIGEYGSVIFIASNIPGKSQIVPLQIVTKLDEFEPAQATAIGVVLL
                     LFSLLILLAINGIETWSRRRSQAAV"
     gene            1625..2665
                     /locus_tag="VGB93_03300"
     CDS             1625..2665
                     /locus_tag="VGB93_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0145180.1"
                     /translation="MTGRVLVAVAILFLTLFIVLPVANVFTQALSNGVAAYWHVFFPP
                     AVDPAAHLTIFQRHRQVAAVRQAVDTWSAIRLSCGVAAIVVPLNIIFGIAAAWAITKF
                     RFRGRLLLLTLIDLPFSISPVIAGLIFVLLFGRGGIVGDWATNVTWPDPSSLSWQGFS
                     GHWWPLVFTNTFTGIIFTPIAIVLATSFVTFPFVARSLIPLMDAQGSDEELAALSLGA
                     SGWRTFWTVTLPNIRWGLLYGAILCTARAFGEFGAVSVVSGHIDSNDTIPLRVEKLWD
                     GYDMQAAFAVSTLLALLAVVTLIIKAAVEIRTKHTETAATAVHEVKPDEYRSQPDQQD
                     LRLVPSAEGRKP"
     gene            2595..>3077
                     /locus_tag="VGB93_03305"
     CDS             2595..>3077
                     /locus_tag="VGB93_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004731677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145181.1"
                     /translation="MSIEVSRISKTYGSFQALKGVSLKVNSGELVALLGPSGSGKTTL
                     LRVIAGIEAADPGGEVLFDGLEVSDTDLGKRKIGFVFQHYALFKQMSVFENIAFGLRS
                     KPWRQRPSNAAIKSKVEELLGLVQLSNFAQRYPSQLSGGQRQRVALARALAVEPQVLL
                     L"
BASE COUNT          499 a          931 c          937 g          710 t
ORIGIN      
        1 tcgccgatgg gaaggcagcg acagatttcc ccatctacaa gaacgccgcg gcgctttcgg
       61 ccgtcactgc gctttacaag catgtgccgg ttctcgatac cggcgggcgc ggcgcgaccg
      121 tgaccttcgc ccagaaacag atcggcgacg tgctggtcaa ttgggagaac gagctctatc
      181 tggcgagcga cgaattcggc aaggacaagt tcgagatcgt ctatccctcc accagcgttc
      241 tcgccgagcc gccggtcgcg gtcgtcgatt ccattgtcga taagaagggc acgcgggcgg
      301 ttgcgacggc ttatctgggc tacctctata cgcccgaggc gcaggatgcg atcgcgcaat
      361 atcattatcg gcctcgcaat atcgatatct ataagaaata cgaagcgact cttccgcccg
      421 tgaagctgtt taccgttgat caggcctttg gcggctggcc gcgtgcgcag aagactttct
      481 tcgccgatgg cggggtcttc gatcaaatct ataagccgac gaagtaagga caccgccttc
      541 gctttggcac aaggctgtgc aattgcggtc ttggaatacg gcggctaaac ggagatcggc
      601 tatattgaat gcggtagcca tcggcttgcg tcttacgaga gtttgaagaa tggcgggcgc
      661 gcgcaaggtc atccccggct tccggctgac gctcggctat acgctcttct atctctcgct
      721 gctggtgctg atcccgctcg cggcgctggt cctcaagacc acgcaattgt cgtggtccga
      781 gttcgtcgcg acgattaccg atccggtcgt cgtcgccgca tatcagttga cctttggcgc
      841 cgcggctctg gccgcggtcg tcaatggtct gttcggtctg atcgttgcct gggttcttgt
      901 gcgtgagcca tttccgggcc ggcgctattt cgatgcgctg atcgatttcc cctttgcgct
      961 gccaaccgcc gtcgccggca tcaccttttc aaatctctat ctcaccgacg gctggattgg
     1021 cggcttcggc gatcatgcag ttgcatttct gaacgggctg gcgggtcatt tcggccaccc
     1081 gaacgctctg tctgccggag cgctgtcctg gctggacttt ccttacagca acaccaacac
     1141 cggcattgtg atcgtgctga tcttcgtcgg gcttccgttc gtcgtccgct cggtgcagcc
     1201 ggtgctgcag gaatgggatc cgcaatatga acaggcggcc tatagccttg gggcaagtgg
     1261 ggcgacgact tttcggcgcg tgatctttcc cgagattttc cctgcgtggc tcagcggcgt
     1321 cgcgcttgcc ttcgctcgcg ccatcggcga atacggctcg gtaatcttca ttgccagcaa
     1381 cattcccggc aagtcgcaga tcgtgccgtt gcagatcgtc accaagctcg acgagttcga
     1441 gccagcccag gccactgcca ttggcgttgt gctgctgctc ttctcgctct tgattctgct
     1501 ggctatcaac gggatcgaaa cctggtcgcg gcggcggtcg caggcggcgg tctgagcgat
     1561 gtccggggtg cgcattctcc aaacagacag cgctccggcg cgttcgaaga agacccggcg
     1621 cggcatgacg gggcgcgtgc ttgtcgccgt cgcgattttg tttctgacgc tgtttatcgt
     1681 tctgccggtc gccaatgtgt tcacgcaggc gctctcgaac ggtgtggccg cctattggca
     1741 tgtgttcttt ccgccggccg tcgatcccgc ggcgcatctc acgatcttcc agaggcatcg
     1801 gcaggtcgcc gcggttcggc aggcagtcga tacgtggagc gcgatccgct tgagctgcgg
     1861 cgtcgccgcc atcgtcgtgc cgctcaacat tatcttcggc atcgccgcgg cctgggcgat
     1921 caccaagttc cgctttcgtg gccggctcct gctgctgacc ttgatcgatc tgccgttttc
     1981 gatctctccg gtcattgccg gcctgatctt cgtgctgctg ttcggacgag gcggaattgt
     2041 cggggattgg gcgacgaatg tgacatggcc ggacccgagt tcactaagct ggcagggctt
     2101 ttccggccat tggtggcctc tcgtctttac caacactttt accggcatca ttttcacgcc
     2161 gatcgcgatc gtgcttgcga cgtcattcgt gacctttccg ttcgtcgcgc gatctctgat
     2221 tccgctgatg gacgcgcaag gctcggacga ggagcttgcc gcgctttcgc tcggcgccag
     2281 cggttggcga acgttctgga cggtgacgct gccgaatatc aggtggggct tgctttatgg
     2341 tgcgatcctg tgcacggcgc gcgccttcgg cgaatttggc gccgtgtcgg tcgtctccgg
     2401 ccatatcgat tccaacgata cgattccgtt gcgcgtcgag aagctctggg atggctatga
     2461 catgcaagcc gcctttgcgg tttcaacctt gcttgcgctg ctggccgttg tgaccttgat
     2521 tatcaaggcc gcggtcgaga tcaggaccaa acataccgag acggccgcga ctgcggtcca
     2581 tgaggtgaag cccgatgagt atcgaagtca gccggatcag caagacctac ggctcgttcc
     2641 aagcgctgaa gggcgtaagc cttaaggtca attcaggcga actcgttgcg ttgcttgggc
     2701 cgagcggctc cggcaagacc acgctgctgc gcgtgattgc cgggatcgaa gcggcggacc
     2761 ctggcggcga agtcctgttc gatggccttg aggtctcgga caccgatctc ggcaagcgca
     2821 agatcgggtt cgtcttccag cattacgctt tgttcaagca gatgagcgtc ttcgagaaca
     2881 tcgccttcgg cctgcgctcg aagccgtggc ggcagcgccc gtcgaacgcc gccatcaaga
     2941 gcaaggtcga ggaactgctt gggctcgtgc agctcagcaa ttttgcccag cgctatccgt
     3001 cgcaattgtc cggcggccag cgccaacgcg tcgcgctcgc ccgcgcgctt gccgtcgagc
     3061 cgcaggtcct attgctc
//
LOCUS       DASWOG010000166         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_223842,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000166 DASWOG010000000
VERSION     DASWOG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223842"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VGB93_03310"
     CDS             <1..628
                     /locus_tag="VGB93_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEY0145182.1"
                     /translation="GYVQNFRQLTELQPAEGPTRISDMHSATFEDGGGKNFSFKMETN
                     VDDAQSDQVDGRAERMKRGVVGVDLVKPKPEQIALGRDVLFPTEHIKYILAAAKAGEN
                     ALEVKVYDGSDSGDKIYDTTTFIGHAIGTPAPDAAAQIPLLQHMRRWPVSISYFDDGR
                     KDEAPNYTLAFDLYENGISRALRLDYGDFVLAGQMVSLKLLPTPPCTK"
     gene            838..1206
                     /locus_tag="VGB93_03315"
     CDS             838..1206
                     /locus_tag="VGB93_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145183.1"
                     /translation="MQKSVLIVEDNELNMKLFNDLLEAHGYRTVQTRSGVEAVELART
                     HRPDLILMDIQLPEVSGLQVTQWIKDDENLRHIPVIAITAFAMKGDEEKIRQGGCEAY
                     LSKPISVVKFLETVRSYIAE"
     gene            1228..>2176
                     /locus_tag="VGB93_03320"
     CDS             1228..>2176
                     /locus_tag="VGB93_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145184.1"
                     /translation="MTAKILVVDDILPNVRLLEARLTAEYFDVTTAMNGAEALAVCAQ
                     DDCDLILLDVMMPGMDGFEVCRRLKKNPATTHLPIVMVTALDQPSDRVKGLEAGADDF
                     LTKPIDEIALIARVRSLTRLKVVLDDLRNRASTAVALGMPDPFTAALAEDGLGGRILL
                     VDDRPASAAQLAFMLRDKYIVEIEPNPNDVAAKVASGAYDLLIVSLGLQGFDALRLCG
                     QLRSQERTRQLPILCLAEMEDRTRVLRALDLGVNDFLMRPVDGNELLARVRTNLRRKR
                     YAEKLRDNVQASLELAVVDSLTGLNNRRYFETHLSTLVAQ"
BASE COUNT          458 a          616 c          634 g          468 t
ORIGIN      
        1 tggctatgtg cagaattttc gtcagttgac ggagctgcag ccggcggaag ggccgacgcg
       61 gatttccgac atgcattcgg cgacgttcga ggatggcggc ggcaagaact tttcgttcaa
      121 gatggaaacc aatgtcgacg acgcgcaatc cgatcaggtg gatggccgcg ccgagcgcat
      181 gaagcgcggc gtcgtgggcg tcgatctcgt caagccgaag ccggagcaga tcgcgctcgg
      241 acgcgacgtt cttttcccga cggagcacat caaatacatt ctggcagcag cgaaagccgg
      301 cgaaaacgcg ctcgaagtca aagtctacga cggctctgac agcggcgata aaatctatga
      361 tactacgacg ttcatcggac acgcgatcgg cacgcccgcg cccgacgccg cagcgcaaat
      421 tccgctgctc caacacatgc gccgttggcc ggtatcgatc agctatttcg acgacggccg
      481 gaaggatgag gcgccgaact acacactcgc gttcgacctt tacgagaacg gcatttcgcg
      541 cgcgctgcgg ctcgattacg gcgattttgt tctcgccggg cagatggtta gcctgaaact
      601 gttgcccacg ccgccctgca caaaatagcg cgaagaacct gttccgccta tgacctgtgc
      661 ctatcccttt ggcacgccta aattttgcgc tccagaggac ggtgacttgc gccacttgcg
      721 cccttgtttc tgcgatccaa ttctcatttc gtaaatttct gtctggtaat cgtcacgttc
      781 atgcttgggt ctcacggcag gaacgtccgc gcccttgagg caataggttc ccaacaaatg
      841 caaaagagcg ttctgatcgt cgaagataat gagcttaaca tgaagctttt caacgacctc
      901 ctggaagcgc acggctatcg gacggtgcag acgagaagcg gtgtggaagc ggtcgagctc
      961 gcccgaacgc atcgtcccga tttaattctc atggatatcc aattgccgga agtctccggg
     1021 ctgcaagtga ctcagtggat taaagacgac gaaaatctgc gccacattcc ggtgattgcg
     1081 attacggctt tcgcgatgaa gggggatgaa gaaaagattc gtcagggcgg ctgcgaggct
     1141 tacctgtcga agccgatttc ggtggtgaaa ttcctcgaaa cggtgcgcag ttacatcgcc
     1201 gaataatccg gcgggggaac gtttctgatg acggccaaga ttctcgttgt cgatgatatt
     1261 ttgccgaacg tgaggcttct cgaagcgcgg cttacggcgg aatatttcga tgtgacgacg
     1321 gcgatgaatg gcgccgaggc gctcgcggtt tgcgcgcagg atgattgcga cctcattctt
     1381 ctcgacgtga tgatgccggg catggacgga tttgaagtct gccgacggct caaaaaaaat
     1441 ccggcgacga cgcatctgcc aatcgtcatg gtaacggcgc tcgatcagcc gagcgatcgc
     1501 gttaagggcc tcgaagccgg tgcggacgat tttctgacaa aacccattga tgaaatcgcg
     1561 ctcatcgcgc gcgtacgttc gctcacccgg ttgaaggtgg tgctcgacga tctgcgtaat
     1621 cgcgcttcga ccgccgtagc tttgggtatg cctgacccct tcactgcagc cctggcggaa
     1681 gacggcctcg gtggccgcat tctgcttgtt gacgaccgtc ccgcgtcagc agcgcaactc
     1741 gcctttatgt tgcgcgacaa atatatcgtc gaaatcgagc ccaatccgaa tgacgtcgcg
     1801 gcgaaagttg ccagcggcgc gtatgacctg ttgatcgtca gtctcgggct tcagggcttc
     1861 gacgctctac gcctgtgcgg ccaactccgc tcgcaggagc gcacacgcca attgccgatc
     1921 ctgtgtctgg ccgagatgga agatcgcacg cgcgtgcttc gcgcgctcga tctcggcgtc
     1981 aacgatttcc tgatgaggcc ggttgacggc aacgagcttc tcgctcgtgt gcgcacaaat
     2041 ctgcggcgca agcgttatgc cgagaagctt cgcgacaatg tgcaggcgtc gctcgaactg
     2101 gctgtcgtcg attctttgac cggactcaac aaccgccgct atttcgaaac ccatctttcg
     2161 acgcttgtgg cgcaag
//
LOCUS       DASWOG010000167         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_150365,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000167 DASWOG010000000
VERSION     DASWOG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150365"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VGB93_03325"
     CDS             complement(<1..1326)
                     /locus_tag="VGB93_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0145185.1"
                     /translation="MTDASDPLPSDLASAHALILAQRELLANEQALRIVAESAAKVGA
                     LEIERLKLMLAKARRAQFGQSSERGRLLIEQLELAIEDLEETQAAEATRVEIAAPEAA
                     REKRARGPRGPRKLPDDLPVERIVEPAPCSCGKCGGRRLRKLGEVVSKTLECEPRRWK
                     IVERVREKFSCRDCEGITEAPAPSHPIPRGFAGPSLLAMVLVSKFLLHQPLNRQSDAC
                     AREGIEIDTSTLADWVGACVVALDPLVQAIRVHVLRADRIHADDTRVQVLAKLKTITG
                     RLWTYVRDDQPFGGTDPPAAFFEYSRNRAGEHPQRHLVDYVGIMQADAYQGYNELYKL
                     ARRPAPIVEAACWAHWRRHFFDLAKDGKAPIAIEAVHRMDELFEIERMINGKSPRERL
                     AVRRELSKPLVDSLETWLSEQRARLSAKNDTTKVINYGLSRWTAFTHFLE"
     gene            complement(1396..>1604)
                     /gene="tnpB"
                     /locus_tag="VGB93_03330"
     CDS             complement(1396..>1604)
                     /gene="tnpB"
                     /locus_tag="VGB93_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465831.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0145186.1"
                     /translation="GRRGDLIKVIWFDDQGACVFSKRLEHGRFLWPSMADGVVTISVA
                     QLSYLLSGIDWRMPRETWRPKAAG"
BASE COUNT          333 a          514 c          454 g          303 t
ORIGIN      
        1 ctccaggaag tgtgtgaagg ccgtccagcg gctgaggcca taattgatga ccttcgttgt
       61 gtcattcttg gcagagagcc gcgcacgctg ctcagacaac caagtctcaa ggctatcgac
      121 cagcggcttc gacagctcac gacggacggc gagacgctct cgaggcgatt tgccgttgat
      181 catacgctcg atttcaaaaa gttcatccat gcggtgcacg gcctcgatcg cgatcggcgc
      241 ctttccatcc ttcgccagat cgaagaagtg tctccgccaa tgcgcccagc acgccgcctc
      301 gacaatcggc gcaggccgac gcgccagctt gtaaagctcg ttatagcctt gataggcatc
      361 cgcctgcatg atgccgacat agtcgaccag atgccgttgc ggatgttctc cggcgcgatt
      421 gcgtgagtat tcaaaaaagg ccgctggcgg gtcagtgccg ccaaaaggct gatcgtcacg
      481 aacgtaggtc cacaatcgcc cggtgatcgt cttcaatttc gcaagaacct gaaccctggt
      541 gtcatcggca tgaatgcggt ctgcccgaag aacatgaact cggatcgcct gaacaagggg
      601 atcgagcgcg acgacgcagg cgccgaccca atcggcgagc gtcgaggtat cgatctcgat
      661 gccctcgcgg gcacaggcat cactttgacg gttcagcggc tgatgcagca ggaacttgga
      721 gacgagcacc atggcaagca ggctcggacc ggcaaaacca cgtgggatcg gatgcgaggg
      781 tgctggcgcc tcggtaatac cttcgcagtc gcggcacgag aacttctccc gcacgcgctc
      841 gacgattttc cagcgccgcg gttcgcattc cagcgtcttg gagacgactt caccgagctt
      901 gcgcaggcgc cgtccgccac atttgccgca ggagcaaggg gccggctcga cgatccgttc
      961 gaccggtaga tcgtcaggca gcttacgcgg tccccgcggg ccacgggcgc gcttttccct
     1021 ggcggcttcc ggcgccgcaa tttccaccct tgtcgcttcg gcggcctgcg tctcctcgag
     1081 atcttcgatt gcaagctcaa gctgctcgat cagaagcctg ccgcgttccg aggattggcc
     1141 gaactgcgcc cgacgggctt tcgccagcat caatttcagg cgttcgatct ccaaggcgcc
     1201 gacctttgcc gcgctctcgg cgacgatcct caacgcttgc tcattggcca aaagctcccg
     1261 ctgcgccaag atcagcgcat gggcacttgc aaggtcggaa ggaagcggat cggaagcgtc
     1321 tgtcacgacc cgatccaacc atattcgcag gcaaaaatcg cctcggatcc tgcgcccgag
     1381 tcaatccgtc acagattatc ccgccgcttt cggacgccac gtctcccgcg gcatacgcca
     1441 gtcgatcccg gaaagaagat aagaaagctg ggcaacactt atcgtaacga caccgtccgc
     1501 catcgagggc cataggaaac ggccatgttc caatcgcttc gaaaacacgc atgccccttg
     1561 gtcatcaaac cagattacct taatgagatc gccgcgccgg ccgc
//
LOCUS       DASWOG010000168         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_40267,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000168 DASWOG010000000
VERSION     DASWOG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40267"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="cysD"
                     /locus_tag="VGB93_03335"
     CDS             <1..510
                     /gene="cysD"
                     /locus_tag="VGB93_03335"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0145187.1"
                     /translation="GARRDEEKSRAKERIFSHRSETHAWDPRNQRPELWRLFNTRIKP
                     GESMRVFPLSNWTELDVWDYTAAESIPVVPLYFAKERPVVDRDGTLIMVDDERLPLRA
                     GESPRPERVRFRTLGCYPLSGAIRSDAETLDGIIAEMRSANTSERQGRLIDHDESGSM
                     EKKKREGYF"
     gene            510..2441
                     /gene="cysN"
                     /locus_tag="VGB93_03340"
     CDS             510..2441
                     /gene="cysN"
                     /locus_tag="VGB93_03340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY0145188.1"
                     /translation="MDQLAEISVLPVVEAENAGANLPALRFLTCGSVDDGKSTLIGRL
                     LYDQQLILDDQLAALDRDSKKHGTVGKDIDFALLVDGLEAEREQGITIDVAYRAFSTP
                     RRSFIVADTPGHEQYTRNMATGASNCDLAILLVDSRKGLLAQTHRHATIVSLLGIRHV
                     VLAVNKIDLVDYDQAVFNKIVADFEAFSTKLNFATIQPIPISARFGENVTEQSEKTPW
                     YDGPTLLDYLETIEVDEDLSGLPFRMPVQAVARPHLDFRGFCGTIVSGTVSVGDPVTV
                     AVSGRESHVARIVTADGDLESAGAGDAVTLTLTDEIDVSRGDVFVPPQARPEVADQFA
                     ASLIWMNDEVLLPGRSYLMKIGTRTVPVSVTAIKYRLDVTSLSEMPAKALALNEVGFC
                     NLATTTPIAFDPYKENHGTGAFILIDRYTNATVAAGLVAHGLRRATNVHLQDLTVTRE
                     ARARLKHQRPTVLWFTGLSGSGKSTIANLLESQLHARGVHTILLDGDNIRHGLNKDLG
                     FTEVDRVENIRRIGEVAKLMTDAGLIVLCAFISPFAADRQMVRDALKADEFVEIFVDT
                     PLEECIKRDPKGLYKKALAGEIKNFTGVDQAYEPPEKAELVLGHDGDSPAQSVARVVG
                     WLVERRHIERFDDSGDWSI"
     gene            complement(2479..2865)
                     /locus_tag="VGB93_03345"
     CDS             complement(2479..2865)
                     /locus_tag="VGB93_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0145189.1"
                     /translation="MKLLLDTHVLLWAAGDPSKLSRKTRALLSAADNELLFSVASLWE
                     ITIKRQLGRADLRVDPRLLRRGLLDNGYGEIGIASAHVVAIDSLPDIHKDPFDRLLIA
                     QARVEGVALLTSDNMVLRYGEPSLKA"
     gene            complement(2862..3104)
                     /locus_tag="VGB93_03350"
     CDS             complement(2862..3104)
                     /locus_tag="VGB93_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009495322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0145190.1"
                     /translation="MRTINIHEAKTHLSRLIELAAQGESFIIAKAGKPMVKVVALTPE
                     VSQTKRLGFMSGQIAIPDDFDQMGAGEIETLFHGDS"
     gene            3569..4075
                     /locus_tag="VGB93_03355"
     CDS             3569..4075
                     /locus_tag="VGB93_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0145191.1"
                     /translation="MSLSQIEFIQTESENPVDQIERIATQNQWAYEREDEDEISICVT
                     GTWTDYNIAFTWLTDIEAVHLACAFDLKIPQARRADALSLIALVNEQLWIGHFDIWPQ
                     DGVVMFRHSILLTGGAVLNASQCEAAMSNAVTACERYYQAFQFVVWAGKSGREALETA
                     LLETKGEA"
     gene            4077..4904
                     /gene="proC"
                     /locus_tag="VGB93_03360"
     CDS             4077..4904
                     /gene="proC"
                     /locus_tag="VGB93_03360"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0145192.1"
                     /translation="MTFSGGGDFPASLLLVGAGRMGGALLKGWLNLGLDPARVHVLDP
                     APSAEMQAFCASRGVKLGAPPSPPEVLVLAIKPQTFEGAAPALAAFIGRETLIVSILA
                     GKTLVRVTAHLPGARAVVRAMPNLPAAIGAGITGVVANAAVTREQKTMAQVLLEAGGA
                     VVWLDDESLIDAVTAISGSGPAYVFYLVECLTKAGLAQGLNPDVAARLARATVEGAGA
                     LLAATPEASPAKLRADVTSPGGTTEAALTELMGGDALADLIGRAVAAGRRRAGQLSG"
     gene            5028..5732
                     /locus_tag="VGB93_03365"
     CDS             5028..5732
                     /locus_tag="VGB93_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0145193.1"
                     /translation="MTEADISQKSAATSPRDAIIGALLELAGERHWEDIPLSDIATRA
                     NVSLSAFRDAFPSKGAVLAAFMRKIDKAVLDDIPAPEPDQSPKDRLFEVLLRRLEVLK
                     PYRPGLESISEWSVRDPFAAAALNKESMNSMRFMLEAAGIDSEGPVGVVKLQGLALAW
                     RRVLATWFTDDSSDLAPTKAVLERELARGSAVLARVEDMGRLASPLFSIARSFLDRRR
                     GGPQETSKDDTPTTFN"
     gene            complement(5802..>6105)
                     /locus_tag="VGB93_03370"
     CDS             complement(5802..>6105)
                     /locus_tag="VGB93_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145194.1"
                     /translation="CLANLGGNALAHAKKIRIDGQRDQRFLTINVDDNGPGIPVDQRE
                     DVFRPFYRLDASRNQDQGGTGLGLAIARDIARSHGGDITLGESPLGGLRATVRVPV"
BASE COUNT         1145 a         1859 c         1870 g         1231 t
ORIGIN      
        1 ggagcacggc gcgacgagga gaagagccgc gccaaggagc gcattttttc acatcgctcg
       61 gaaacccacg catgggaccc gcgcaatcaa cgcccggaac tttggcgtct gttcaatacg
      121 cgcatcaagc ctggcgaatc gatgcgggtg ttcccgctgt cgaattggac tgagctcgat
      181 gtctgggatt acacggcggc ggaatcgatt ccggtcgtgc cgctctattt tgccaaagag
      241 cgtccagtcg ttgaccgcga cggcacgctg atcatggttg atgacgagcg cctgccgctg
      301 cgggcgggcg agtcgccgcg gccggagcgc gtccgcttcc gcaccctcgg ctgctatccg
      361 ctctccggcg ccatccgctc ggatgcggaa acgctggatg gcatcatcgc cgaaatgcgc
      421 agcgccaaca cctcggaacg ccagggccgg ctgattgacc acgatgaatc cgggtcgatg
      481 gaaaagaaaa agcgcgaagg ttatttctga tggaccaact cgccgaaatc agcgtcttgc
      541 cggtcgtcga ggcggagaat gcgggggcca atctgccggc gctgcggttc ctcacctgcg
      601 gctcggtcga cgatggaaaa tcgacgctca tcggccgcct gctttacgat cagcagctca
      661 ttctcgacga tcaactcgcg gcgctcgatc gcgattcaaa gaagcacggt actgtcggca
      721 aggacatcga tttcgcgctg cttgtcgacg ggcttgaggc cgagcgcgag cagggcatca
      781 ccattgatgt cgcctatcgt gccttctcga cgccgcgccg ttcattcatc gtcgccgata
      841 cgcccggcca tgaacaatat acgcgcaaca tggcgaccgg cgcgtccaat tgcgatctcg
      901 ccatcctgct tgttgattcg cgcaagggcc tgctggccca gacgcatcgc catgcgacta
      961 tcgtctcctt gctcggcata cgccatgtcg tactggccgt gaacaagatc gatctcgtcg
     1021 attacgatca ggcggtcttc aacaagatcg tcgccgattt tgaagcgttc tcaaccaagc
     1081 tcaatttcgc gacgatccag ccgattccga tttcggcgcg gttcggcgag aacgtcacgg
     1141 agcagagcga gaagacgcca tggtatgatg ggccaacgct gctcgattat ctcgaaacaa
     1201 tcgaggtcga tgaagatctg agcggcttgc cgttccgcat gccggtgcag gccgtcgccc
     1261 gaccgcatct cgactttcgc ggtttttgcg ggaccatcgt atccggcaca gtgagcgtgg
     1321 gcgatccggt gacggtcgca gtttcagggc gtgaatcgca tgtcgcccgt atcgtcactg
     1381 ctgacggcga tctcgaaagt gccggcgcgg gggatgccgt caccctgacg ctcaccgacg
     1441 aaattgacgt aagtcgcggc gatgtttttg tgccgccgca ggcaagaccg gaggttgccg
     1501 accagtttgc cgcctcgctt atctggatga atgacgaggt gctgttgccc ggccggtcgt
     1561 acctcatgaa gatcggcacc cgcacggtgc cggtttcagt gaccgcgatc aaatatcggc
     1621 tcgacgtgac aagtctcagc gagatgccgg ccaaagcgct ggcgctcaat gaagtcggct
     1681 tctgcaacct tgccacgaca acgccgatcg ccttcgatcc ctataaagaa aatcatggca
     1741 ccggtgcttt catcctgatc gaccgctaca ccaatgcgac ggttgcggcg ggtctcgtcg
     1801 cgcatggtct ccggcgcgcc accaatgtcc atcttcagga cttgaccgtt acccgcgaag
     1861 cccgcgcgcg gctcaagcat cagcgcccga cggtgttgtg gttcaccggc ctttccggtt
     1921 cgggcaaatc gactatcgcc aatctgctgg agagccagct tcatgcccgc ggcgtccata
     1981 cgatcctgct cgacggcgac aatatccgcc atggtctcaa caaggacctt ggcttcaccg
     2041 aggtcgaccg cgtcgaaaac attcgccgca tcggcgaggt cgccaagctg atgaccgatg
     2101 cggggctcat cgtgctttgt gccttcattt cgcccttcgc cgccgaccgg cagatggtgc
     2161 gcgacgcatt gaaggcggac gagttcgtcg aaatcttcgt cgatacgccg cttgaagaat
     2221 gtatcaagcg ggatccgaag ggtctctaca agaaggcgct cgccggcgag atcaagaatt
     2281 tcacgggcgt cgatcaagct tatgagccgc cggagaaggc cgagcttgta ctcggccacg
     2341 acggcgattc gccggcgcaa tcggtcgccc gtgttgttgg ctggctggtg gagcgccggc
     2401 atatcgagcg gttcgacgat tcaggcgatt ggtcgattta aagcatcaga ccgaaaagtg
     2461 cgtagcagtt ttcatacctc atgccttgag gctaggctcg ccgtagcgca gcaccatgtt
     2521 atctgatgtc agcagagcga cgccctcaac gcgcgcctga gcgatcagga ggcgatcaaa
     2581 cggatccttg tgaatatccg gcagcgaatc gattgcgaca acatgcgcgc tcgcgatgcc
     2641 gatttcgcca tagccattat cgagcaagcc gcgccgcagg agccgcggat cgaccctcaa
     2701 gtccgcgcgg cctagttggc gtttaatggt aatttcccaa aggctcgcga cgctgaaaag
     2761 caattcattg tcggcagccg acaacagtgc gcgcgttttg cgcgacagct ttgagggatc
     2821 accagccgcc cataaaagga catgcgtatc gagcagcagc ttcacgaatc accgtgaaag
     2881 agcgtctcga tctcccccgc gcccatctga tcgaagtcgt cgggtatggc aatctgaccg
     2941 ctcatgaatc cgaggcgttt ggtctgcgag acctccggcg tcaacgcaac gacctttacc
     3001 atcggctttc cggccttggc gatgatgaac gattcgcctt gcgccgccag ttcgatgagg
     3061 cgcgacagat gcgtctttgc ctcatgaata ttgatggttc gcatttgggt gccaatataa
     3121 acttagtcta ctagacttag tttacaaaag tcgccccgtc aaccgtccgc cggtttttgc
     3181 ttatccaccg tttttgcggc gcttcgcgca taagctttgg gcaagccttg cgctttgcct
     3241 tgccgctcat gcgcggcaag ccccccttcg acgctctggc gaatcgcggg gcctttgctg
     3301 acgcggcggc cgcacacaag gccgcaggat agttttgtcc gtgttatccc cgtcgtcacc
     3361 atgttgcaga atcacctgac gatgtgattc agtttcaggg ttcgcgatat gagtcgccgt
     3421 gcggtcgggc cctgacgatg gaaattgccg cgcttagagc cttggagcga agtgtagtgt
     3481 gtcgccgttg cgtgagccgt ctcgggaaat ctcccgtggc agtgaaattt tgatcgcggc
     3541 gccagccgcg acgtcccagg gcaaaggcat gtcgctctcg cagattgaat ttatccagac
     3601 tgaatccgaa aatcccgtcg accagatcga aaggatcgcc acccaaaatc aatgggccta
     3661 tgagcgcgag gacgaagacg aaatctccat ttgcgtcacc ggcacctgga ccgattataa
     3721 catcgcattc acttggctca ccgatattga ggcggtgcat ctcgcttgcg ctttcgatct
     3781 taaaattccg caagcgcgcc gcgccgatgc tctttcgctg atagcactcg ttaacgaaca
     3841 gctgtggatc ggccatttcg atatctggcc gcaggacggt gttgtcatgt tccggcactc
     3901 gatcctgctg acgggcggcg ccgtgctcaa tgccagccaa tgcgaggccg ccatgagcaa
     3961 tgcggtgacc gcatgcgagc gctattatca agccttccaa ttcgtggtgt gggccggtaa
     4021 gagcggccgt gaggcgctcg aaaccgccct gctcgaaacc aaaggcgaag cctgaaatga
     4081 ccttttctgg cgggggagat ttccccgcct cgctgctgct cgtgggcgcc ggccgcatgg
     4141 gcggtgcttt gctgaagggc tggctcaatc tcgggctcga tccggcacgg gtgcatgtcc
     4201 tcgatccggc gccgtcggcg gagatgcagg cgttttgcgc ctcgcgaggc gtgaaactcg
     4261 gcgcgccgcc ttcgccgccc gaagtcctcg ttctcgctat caagccacag acgttcgagg
     4321 gcgccgcacc ggccttggcg gcttttatcg gccgtgaaac gcttattgtc tcgatcctcg
     4381 ccggcaagac gctggtgaga gtgaccgctc atttgcccgg cgcgcgtgct gtggtccgcg
     4441 ccatgcctaa tctcccggcc gcaatcggcg ctggcatcac cggcgtagtc gccaatgccg
     4501 cggtcacgcg tgagcaaaag acgatggctc aggtgctcct cgaagccggc ggcgccgtcg
     4561 tctggctgga cgatgaaagc ctgatcgatg ccgtgaccgc aatttcgggg tcgggaccgg
     4621 cctatgtctt ttatctggtc gaatgcctga cgaaggccgg cctcgcgcaa ggacttaatc
     4681 cggacgtcgc cgcgcgtctg gcgcgcgcga cggtcgaggg cgctggcgcg ctgctggcgg
     4741 cgacgcctga agcgtcgccg gcaaaattgc gcgccgatgt gacctcgccc ggcggaacca
     4801 ccgaagcggc cctcacggag ttgatgggcg gcgacgcctt ggccgatctc atcggccgcg
     4861 ccgtggcagc gggccgccga cgcgcggggc aattgtccgg ctgacgcaac ccgtcggcat
     4921 taccgagcgt ttgccggagc ttccaaattg ctgtgccgcg cgatgtctgg cttgactggc
     4981 gggcgccaca gcctagtttc catccgtccc tagcgcagga ggtcagcgtg accgaggccg
     5041 atatcagcca gaaatccgca gccacgtccc cgcgtgacgc tattatcggg gctctgctcg
     5101 aacttgccgg cgaacggcat tgggaagaca tcccactcag cgacatcgcc acgcgcgcca
     5161 atgtgtcgtt gtctgcgttc cgtgatgcat ttccatcgaa gggtgcagtg ctcgcggcct
     5221 tcatgcgcaa gatcgataag gcggtgctcg acgacatccc agcgcctgag cccgaccaat
     5281 cgcccaagga tcggcttttt gaggtgctcc tgcgccgtct cgaggttttg aaaccttacc
     5341 ggccgggact tgagagcatc agcgaatgga gcgtgcgtga tccgttcgcg gcagcggcgc
     5401 tgaacaagga atcgatgaat tccatgcgct tcatgctgga ggcggcgggt atcgattcgg
     5461 aaggtcctgt tggcgtggtg aagctgcaag gcctcgcctt ggcgtggcga cgcgtgctgg
     5521 cgacgtggtt cacggacgac agctccgatc tcgcgccgac gaaggcggtg cttgaacgcg
     5581 agctcgcgcg cggtagcgcc gttcttgctc gcgtcgagga catgggccgt ctggcctcgc
     5641 cgctgttctc gatcgcgcgc tcgtttctcg accgtcgccg tggtggtccg caggagacga
     5701 gcaaagacga cacaccgacg acgtttaact agagcaccgg accgaaaagt gcgcagcggt
     5761 tttccggcca acccgatgcg attagagaga tggtcgcgtc gttaaaccgg cacgcggacc
     5821 gtcgcgcgca aaccgccgag gggactttcg ccaagcgtga tgtcgccacc gtgcgagcgc
     5881 gcgatatcgc gggcgatggc gaggccgagc cccgtgccgc cctggtcctg attgcgcgag
     5941 gcatcgagcc ggtagaatgg gcggaagacg tcttcgcgct gatccaccgg aattccgggg
     6001 ccgttatcat cgacgttgat agtgagaaac cgctgatcgc gctggccgtc gatgcggatc
     6061 ttcttcgcat gtgccaaggc attgccgccg aggttggcga gacag
//
LOCUS       DASWOG010000169         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_187427,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000169 DASWOG010000000
VERSION     DASWOG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187427"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1086
                     /locus_tag="VGB93_03375"
     CDS             160..1086
                     /locus_tag="VGB93_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145195.1"
                     /translation="MPPVISVSHLSKTYAGGRAALKVVSLEIGKGEIFALLGPNGAGK
                     TRLISIICGIVTPTAGTITAAGHDIIRDFRAARAMIGLVPQELTIDFFESVWATVKLS
                     RGLFGKAPDDAYLESLLRDLSLWDRKDSQARTLSGGMKRRMMIAKALSHQPEILFLDE
                     PTAGVDVELRRGMWELVRKLRAAGTTIILTTHYLEEAEEMDDRVGVIRQGELILVEDK
                     KKLMLKLGKKKLTLMLAAPLAALPASLQAYPLSLSDDGRELTYTYGEDGGAAPVAALF
                     DSLRTAGIEVSDLRTEQSSLEDIFVSLIGKTA"
     gene            1083..1844
                     /locus_tag="VGB93_03380"
     CDS             1083..1844
                     /locus_tag="VGB93_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0145196.1"
                     /translation="MNLQAIRAIYVYEMSRTRRTLLQSIVSPILSTALYFVVFGSAIG
                     SRISHVGGVSYGAFIVPGLIMLNLLTQSISNASFGIYFPRFVGTIYEILSAPVSAVEI
                     VIGYVGASASKSIVLGLLTLATAALFINMHILHPFWMLAFLVLTSVTFSLFGFVIGIW
                     ADSFEKLQLIPLLIVTPLTFLGGSFYSIDMLPPFWRNVTLINPVVYLISGFRWSFYGL
                     SDINVGISLGMTLLFLVLCLGIVVWIFKTGYRLKS"
     gene            complement(1897..2535)
                     /locus_tag="VGB93_03385"
     CDS             complement(1897..2535)
                     /locus_tag="VGB93_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HEY0145197.1"
                     /translation="MPKFAMPTFKILAAFAVALCLCGGPALARHHHHHHYDDDNAAVA
                     SGLAGDTLLIVRHAEKPDGEGNPGLAPAGEARAKAYADYFAHLQIDGAPVKIGTIIAS
                     ADSDDSERPRLTVTPFSQASGIKIEQPFPDKEVKALAHWLAAGTPNRTILIAWHHGKL
                     PKLLRELGADSEDLVPGGVWPEDTYNWLIVLKYDSSGTLTEAKKIVEPEFAK"
     gene            complement(2550..2912)
                     /locus_tag="VGB93_03390"
     CDS             complement(2550..2912)
                     /locus_tag="VGB93_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0145198.1"
                     /translation="MFDHIGIHTNQFDALISFYEAALAPLGYGKLESFEDAAAFGTDK
                     PALWIGTVEAKPVGIHIALSAANPAAVDRFYAAAIAAGGKDNGAPGPRPDYAPGYYAA
                     FIIDPDGNNLEAVHRQPA"
     gene            complement(2927..>3292)
                     /locus_tag="VGB93_03395"
     CDS             complement(2927..>3292)
                     /locus_tag="VGB93_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEY0145199.1"
                     /translation="AETDRMTFPQSVAKLTDAGFDGYLVDLRRATRTFYLPDGESLDL
                     STAKMAQNVASRFDVESIRAAIREAQALAPGYTYRGFCVKVAAAGCAGYLVSFPERRV
                     LYFGRTGETHVEHFPGAQP"
BASE COUNT          617 a          995 c          983 g          697 t
ORIGIN      
        1 aaggcgatcg aggctacggc gaggacagcg cgaagagtga actttcccta ttctctgatc
       61 cgtgagacgc ccgcgcttta gccgattggc caaacgccat gcagatgggc taaaagcctg
      121 cttcgtttcg accctcaccg ccgtcacagt gtcttgccaa tgccccctgt catttccgtc
      181 agtcatctgt cgaagaccta tgccggcggc cgcgcggcgc tgaaggtcgt gagccttgag
      241 atcgggaaag gcgaaatttt cgccctgctc ggccccaacg gcgccggcaa gacgagactg
      301 atcagcatca tctgcggcat cgtcacgccc accgccggca cgatcacagc agcgggccac
      361 gacatcattc gtgatttccg cgccgcgcgc gcgatgatcg gccttgtgcc gcaggaattg
      421 accatcgatt ttttcgagag cgtgtgggcg acggtcaagc tcagccgggg cctcttcggc
      481 aaggcgccgg acgatgccta tctcgaaagt ctgctgcgcg acctctcgct ctgggaccgt
      541 aaagatagcc aggcgcgcac actttccggc ggcatgaagc ggcgcatgat gatcgccaag
      601 gcgctttcgc atcagcccga aatccttttc ctcgacgagc cgacagccgg cgtcgatgtc
      661 gaacttcgcc ggggcatgtg ggaattggtg cgcaaactgc gcgccgcagg cacgacgatc
      721 atcctcacca cgcattatct cgaagaagcc gaggagatgg acgaccgcgt tggcgtcatc
      781 cggcagggcg agctgattct cgtcgaagac aagaaaaagc tgatgttgaa gctcggcaag
      841 aagaagctga cgctcatgct cgccgcgccg ctcgccgccc tgcccgcttc gctgcaagcc
      901 tatccgctga gcctttcgga cgacggccgc gaattgacct atacctacgg cgaagacggc
      961 ggcgccgcgc ctgtcgcggc tctgttcgac agtttgcgca cggccggcat cgaagtcagc
     1021 gatctgcgca ccgagcagag ttcgctcgaa gacattttcg tcagcctcat cggaaagaca
     1081 gcatgaattt gcaggcgatc cgggcgatct acgtctacga gatgtcgcgc acgcggcgca
     1141 cgctgctgca gagcattgtc tcgccaatcc tgtcgaccgc gctctatttc gtggtcttcg
     1201 gctctgcgat cggctcgcgc atctcgcatg tcggcggggt gagctatggc gccttcatcg
     1261 tgccggggct gatcatgctc aacctgttga cgcagagcat cagcaacgcc tcgttcggca
     1321 tctattttcc acgcttcgtg ggaacgatct atgagatctt gtcggcgcct gtctcggccg
     1381 tcgagatcgt catcggttac gtcggcgcgt cggcgtcgaa atcaatcgtc ctcggcctgc
     1441 tgacgctcgc caccgcagcg ctcttcatca acatgcatat cctgcatccg ttctggatgc
     1501 tggcgtttct ggtgctcacc agtgttacgt tcagtctgtt cggcttcgtc atcggcatct
     1561 gggcggacag tttcgaaaag ctacaattga tcccgctctt gatcgtgaca ccgctcacct
     1621 tccttggcgg cagcttctac tcaattgata tgttgccgcc gttctggcgc aacgtgacgt
     1681 tgatcaatcc cgtcgtatat ctcatcagcg gcttccgctg gagtttctac ggcctctccg
     1741 acatcaacgt cggcattagc cttggcatga cgctgctgtt tctcgtgctc tgccttggaa
     1801 ttgtggtctg gatcttcaag acgggctacc ggctgaagtc gtagttgcgc cgtcattgcg
     1861 agcggcgcga tacgcctcgc aatgacgagg cgttgcttac ttggcgaatt cgggctcgac
     1921 gattttcttg gcctcggtaa gggtgccgct gctgtcgtat ttgagcacga tcagccaatt
     1981 gtaagtatct tccggccaca cgccgccggg aacgaggtct tcactgtctg caccgagttc
     2041 gcgtaagagt ttcggcagtt ttccgtgatg ccaggcgatg agaatggtgc gattgggcgt
     2101 gcccgccgcg agccaatgtg cgagcgcctt gacctccttg tcgggaaacg gctgctcgat
     2161 cttgatacca ctggcctgac tgaacggcgt gacggtcaga cgcgggcgct cgctatcgtc
     2221 cgaatccgcc gaggcgatga tcgtcccgat cttcaccggc gcgccgtcga tctgcaaatg
     2281 cgcaaaataa tcggcgtaag ccttggcacg cgcttcgccc gcgggcgcaa gcccgggatt
     2341 gccttcgccg tcgggctttt cggcgtggcg cacgatcagc aacgtatccc cggcgaggcc
     2401 cgaggcaacg gctgcgttat catcgtcgta gtgatgatgg tgatgatggc gcgcaagcgc
     2461 ggggccgccg cagagacaga gcgcgacagc aaaagcggcg agaattttga atgtcggcat
     2521 tgcaaatttt ggcatcgaca gttccgtgtt caagcaggtt gccggtggac ggcttcgaga
     2581 ttgttgccat ccgggtcgat aatgaaagcg gcgtaatagc ccggcgcata atccggacgc
     2641 ggcccgggcg ctccgttgtc tttgccgccc gcggcaattg cggcggcata aaagcgatcg
     2701 accgcagccg ggttggcagc ggacagcgcg atgtggatgc cgacgggctt tgcctcgacg
     2761 gtgccaatcc aaagtgccgg tttgtcggtt ccgaatgccg cggcgtcttc gaagctttcg
     2821 agcttaccgt agccaagcgg cgccaaagcg gcctcgtaga aagagatcaa ggcgtcgaat
     2881 tggtttgtgt ggatgccgat atgatcgaac atgcttgagc ctctgcctag ggctgcgcgc
     2941 cgggaaaatg ttccacatgc gtttcgcccg tgcgaccaaa ataaagcaca cgtcgctctg
     3001 ggaaggagac gagatagccg gcgcagcccg ctgcggcgac cttgacgcaa aagcctcgat
     3061 aagtatagcc gggcgccaaa gcctgagcct cgcggatcgc cgccctgata ctctccacat
     3121 cgaagcgtga cgccacgttt tgagccatct tcgcagttga aagatcgagg ctttcgccat
     3181 ccggcagata aaacgttcgt gtcgcgcggc gcagatcgac aagataaccg tcgaagcccg
     3241 cgtccgtcag ctttgcgacg ctttgcggaa acgtcatccg gtccgtctcc gc
//
LOCUS       DASWOG010000170         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_333760,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000170 DASWOG010000000
VERSION     DASWOG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333760"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..826)
                     /gene="pdhA"
                     /locus_tag="VGB93_03400"
                     /pseudo
     CDS             complement(1..826)
                     /gene="pdhA"
                     /locus_tag="VGB93_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736223.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
     gene            complement(1119..1466)
                     /locus_tag="VGB93_03405"
     CDS             complement(1119..1466)
                     /locus_tag="VGB93_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0145200.1"
                     /translation="MVIRRRVRAVLLPLALYCLSGAAGSYFLWHAVNDDRGLRANEDY
                     ERQIRSLRTQVDAKEKERQAWARRIALLSGATIDRDMLDEEARARLGRVNKDDLIVLL
                     KPNPDAQSAPATP"
     gene            1609..>2502
                     /locus_tag="VGB93_03410"
     CDS             1609..>2502
                     /locus_tag="VGB93_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0145201.1"
                     /translation="MAHRVGADLPDTRAGLSLEAADFAHALFTRSGALTGWNGAFAAN
                     FPATELHRDAHISAILPRIDNELWAQTWLKLDAGQTVTLRIRSGSADPNCDVCITPVF
                     GAQGRLASVEIRHPAASNLQSLQQLQREILEVVATGWPLRDVVDLLCRSAEAMSPGVI
                     CSVLRADLGANCLYPLAGPSLPKQIQDATECVPIGPGMGSCGNAAFYGEATEATDIST
                     DPRWEPFKNLFLPHGLRACWSTPIKGRSGQVLGTFAFYYREPRGPDMLERQIVEACVH
                     LCAIAIDHDYAHSRILSLAYRD"
BASE COUNT          528 a          747 c          680 g          547 t
ORIGIN      
        1 cgtcgatatt cttcaatgcc gactcgtttg cctgatggcc gttgagaaga cgttcgcgaa
       61 cctgctcgat cggatcgctc tcggtgcgca ttttctgcac ctcttctttc gaccgatact
      121 tagcggggtc ggacatggaa tggccgcgat agcgataggt catcatctag agaatgattg
      181 gccctttgcc gtcacggcac cattgcgccg cctgctccgc cgccgccgcg acggcgcgca
      241 cgtccatgcc gtccacctgc tcgcctggaa tattgaacga aaggccgcgc ttggagaaat
      301 ccgtcatcgc cgacgagcgc ttgaccgatg tgcccatggc gtagcgattg ttttcgatca
      361 catagacgac gggcagcttc cacagctccg ccatgttgaa gctctcgtaa acctggccct
      421 gattagccgc gccgtcgccg aaataggtga agctgacgtt gctattgtcg ttgtagcgat
      481 tggcgaaggc gaggccggtc ccgagcggca cttgcgcgcc aacgatgcca tggccgccgt
      541 agaaatgatg ttccttggag aacatatgca tcgagccgcc cttgcctctg gaaatcccat
      601 cacggcggcc ggtcaattcc gccattacgg ctttcggtgg gatgccgcag gcgagcatgt
      661 gcgcgtggtc acggtagccg gtgatggtct ggtcgcccgg cttcgccgaa cgcatcacgc
      721 cgacgacgac cgcctcctgc ccgatgtaaa gatggcagaa accgccgatc aggcccatgc
      781 catacatttg gccggccttc tcctcgaagc gacggatcag cagcatttcg cggtaaactg
      841 cgagttcttg atcgagggta aattgcgcga gccccgcatt gggaaggctc aaatggctta
      901 tgtctgccgc agcggtcgag ccgttgactt ggctccctga tttcgctgcc atcatttctc
      961 ctccgaaggc gcgcagcaaa gctaacttgc gccttcgcat aaaagcgaag ctctatcgat
     1021 tccgctttta gtttttaccc taacggcgca ggcgaatcca cgcgcaaact atcccctttg
     1081 cgcaaaagat ttttacggcg aagggcattt tccagagctt agggcgtagc cggcgctgat
     1141 tgggcgtccg gattaggctt aagcaggacg atgaggtcgt ccttattgac tcgtccgagg
     1201 cgcgcccgtg cctcctcatc aagcatatcg cgatcaatcg tggcgccact caagagcgcg
     1261 atacgccgag cccaagcctg gcgctctttt tccttcgcat caacttgagt acgtaagctt
     1321 ctgatctgac gctcataatc ctcgtttgcg cgaaggccac ggtcgtcgtt gacagcatgc
     1381 caaagaaaat agctgccggc agcaccggat aagcaataga gcgcgagagg aagaagaaca
     1441 gcgcgaacgc gacgacgaat aaccattctc taagcttcgc cgatcaaagt tagcgcaccc
     1501 ttaaacggcc gtaccaatca gttgagcaat cagccaagtt caacgtaacc aaacctttat
     1561 tgctatgtcg taaaatggat aaataagaga acaaagcacg agctttttgt ggcacatcgc
     1621 gttggcgccg atctacccga tactcgtgct ggcttgtccc ttgaagccgc cgattttgcg
     1681 cacgcgcttt tcacgcgttc cggggcactg acagggtgga atggcgcatt cgctgccaat
     1741 ttcccggcaa ccgaacttca ccgcgacgct catatctccg ctattcttcc acggattgac
     1801 aacgagcttt gggcgcagac ctggctaaag cttgatgcgg ggcaaaccgt caccctgcga
     1861 ataaggtcag gctccgccga cccaaattgc gatgtctgca tcacaccggt cttcggcgcc
     1921 caagggcgtc tcgcatcggt cgaaatcagg catcccgcag cgagcaattt gcaatcgctg
     1981 cagcaattgc aaagagaaat tcttgaagtc gtggcaaccg gctggccatt gcgggatgtc
     2041 gtcgacctgc tctgccgcag cgccgaagcg atgtcgccgg gcgttatctg ttccgtcctg
     2101 cgcgccgatc ttggcgccaa ttgcctttat ccattagccg ggccaagctt gccaaagcaa
     2161 atccaggacg ccaccgaatg cgtgccgatc ggacccggca tgggttcatg cggaaacgcc
     2221 gctttttatg gcgaagccac cgaagcgact gacatctcga ccgatccacg ttgggaaccg
     2281 ttcaaaaatt tatttctgcc acatggtctg cgcgcctgct ggtcaacgcc gatcaagggt
     2341 cgcagcggac aagtactcgg cacgttcgcg ttctattacc gtgagccgcg cggaccggat
     2401 atgctcgaac ggcaaatcgt cgaagcctgc gttcatctgt gcgcgatcgc aatcgatcat
     2461 gattacgcgc actcgcgtat tctgagcctt gcttatcgcg at
//
LOCUS       DASWOG010000171         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_315810,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000171 DASWOG010000000
VERSION     DASWOG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315810"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1335
                     /locus_tag="VGB93_03415"
     CDS             115..1335
                     /locus_tag="VGB93_03415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145202.1"
                     /translation="MSLTRALARSYRMEQVGRRQILAGMGAVLSASALGGCGVGNSPA
                     NRGPALQLARILASPDRITNITVCTRPFRAQGPRLDVEQIGQKTVVHNYGHGGSGWSL
                     SWGSSAIAVQKAMATGERDIAVIGCGALGLTSALLLQRAGAEVTIYAKDLPPNVRSSL
                     ATGIWTPDSRICFEDYAAPAFKQLWAKMARESFRTYQTLLGLPGDPVEFIDDYFVSDN
                     PDATPHHIASGDKRPKFADLQRELIGDLIPRSEVFAPGSHPFGARYLRRNTFMMFNLS
                     DYTRMLIADFQSYGGKIEVDEFHTPADFGRLSQKTLINATGYGARALFGDQSITPVRG
                     QLARAIPQPDVHYGLDFNGVYFVPRRDGLVFQVHGQNDYYGFGDASTVPDRNEALLAV
                     NTMASLWPHEPLNE"
     gene            complement(1445..1669)
                     /locus_tag="VGB93_03420"
     CDS             complement(1445..1669)
                     /locus_tag="VGB93_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY0145203.1"
                     /translation="MPDRDATAISKHSLTIAGHRTSISLEQAFWEKLRAIASARGLSL
                     AALIGEIDAVRGKANLSSAIRVYTLNYSSR"
     gene            complement(1662..1859)
                     /locus_tag="VGB93_03425"
     CDS             complement(1662..1859)
                     /locus_tag="VGB93_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEY0145204.1"
                     /translation="MSEIVNLRRARKAKGRADAADKAAENRLRFGAPKAERERAAATR
                     ELERRRLEGHRRESEPGSEDA"
     gene            complement(1856..2509)
                     /locus_tag="VGB93_03430"
     CDS             complement(1856..2509)
                     /locus_tag="VGB93_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY0145205.1"
                     /translation="MASDLIRYDLLVQEALRSVLRKVLADAARDGLPGNHHFVIVFRT
                     DAPGVRIPAKLREKYPQEMTVVLQHEFWDLAVTEQHFEVRLHFDRKPELLVIPFTAVT
                     GFFDPSVEFGLKFEMANEAPTATSQPAIAPQAPALRSVPPEKSKPRGSGSEPAEINPK
                     QADLGEAAKPSLGKGLGRPELSKSELGKSTKPELAKSDDTPAESPTKVVSIDAFRKK"
     gene            complement(2532..2884)
                     /gene="ssrA"
                     /locus_tag="VGB93_03435"
     tmRNA           complement(2532..2884)
                     /gene="ssrA"
                     /locus_tag="VGB93_03435"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            2992..3765
                     /locus_tag="VGB93_03440"
     CDS             2992..3765
                     /locus_tag="VGB93_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY0145206.1"
                     /translation="MDATPEMQSKRAPYGIIGLCLSIVAAIVLTLIVMAAAAAAVYFG
                     DAALHGMGDARTKARAVLSTLQIDDQFGAARLALGIFFYVAALASILLVACWRAGRDW
                     RTLVAWRVPLWPMRDKILWGIVAIGLIYDLASSAALAHFYPQSNSWLQMPHGHMAIAL
                     LLVVAVIVAPVVEETYFRGWIFTSLRQSWGRWPAIIISALLFGLAHYESTHLYALAVF
                     PLGLILAALRERTGSAGTSMLFHAANNLIAVLSAAASST"
     gene            3788..>4368
                     /gene="thyA"
                     /locus_tag="VGB93_03445"
     CDS             3788..>4368
                     /gene="thyA"
                     /locus_tag="VGB93_03445"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0145207.1"
                     /translation="MRHPETQYLDLLEQILEHGDRRVDRTGVGTLSIFGALTRFSLAD
                     GTVPILTTKRVYWKTAVKEMLWFLTGGTNIQPLLKNNVHIWTDWPLDRYRKETGGAIS
                     QSEFEQKIIDNDVFAARWGGLGPVYGKQWRRWLGPDGKEHDQIAALIKTIKTNPSSRR
                     MLFHAWNVAEIDQMSLPPCHLIYQFYCTQDGRL"
BASE COUNT          828 a         1295 c         1287 g          958 t
ORIGIN      
        1 agtgccgcgg ggcgttatta atgtcgttgc cccatcgtca atttttgggc ttcgcggcgg
       61 cctcttgtcg atgagaacat ttatcggcaa actgtccagg tcgcgctaat ttgtgtgtct
      121 ctcacacggg cgctcgctcg aagctatcgc atggaacagg tcgggcgccg tcagatactt
      181 gctggaatgg gcgccgtgct ttcggcaagc gccctcggag gctgtggcgt cggcaattcg
      241 cctgctaatc gcggacccgc actccaattg gcgcgcatcc tcgccagtcc tgatcgcatt
      301 accaatatca ccgtctgcac acgtcccttc cgcgcacagg gtccgcggct ggatgtcgag
      361 cagattggac aaaagaccgt cgttcacaat tacgggcatg gcggcagcgg gtggtcgcta
      421 tcgtgggggt cgagcgccat cgccgttcag aaggccatgg ccaccggcga gcgggacatc
      481 gcagtgatcg gctgtggcgc tttggggctc acgtcagccc ttctgctcca gcgtgccggc
      541 gccgaagtta ccatctatgc caaggatctg ccgccgaacg tccgttcttc attggccacc
      601 gggatatgga ccccggattc gagaatctgc ttcgaagatt acgccgcacc ggccttcaaa
      661 caattgtggg cgaaaatggc ccgcgagtct ttccgaacct atcagacctt gcttggcctg
      721 ccaggcgatc cggttgaatt catcgatgat tactttgtct ccgacaaccc ggatgccacg
      781 cctcaccaca ttgcgtccgg tgataagcgg ccgaaattcg ctgatcttca acgcgaactc
      841 attggcgatc tcatcccgcg aagtgaggtt ttcgcacctg gcagtcaccc gttcggcgcc
      901 cgctatctgc ggcgcaatac gttcatgatg ttcaatcttt ccgactatac gcggatgttg
      961 attgcggatt ttcagtccta cggcggtaag atcgaggtcg atgaatttca cacacctgcc
     1021 gatttcggcc gattgagcca aaagacttta atcaacgcca cgggctatgg cgcccgtgct
     1081 ttgttcggcg accagtcgat cacgccagtg cgcggccagc tggcccgtgc gattccgcag
     1141 ccggacgttc actacgggct cgatttcaac ggggtctact tcgtccctcg acgggacggc
     1201 ctggtctttc aagtgcatgg ccaaaacgat tattatggtt tcggcgatgc cagcaccgta
     1261 cccgaccgaa acgaggcttt gcttgcggtc aataccatgg cttcactttg gccacatgag
     1321 ccgctgaatg agtagtctag cctactttgg tcgttagccg tcgcgtttac catggctgca
     1381 aatgaataac gacgcggtgc ccaacagatt gggcgcgggt ctataggcag acttgcaaag
     1441 agttttatct acttgaataa tttagagtat agacgcgaat ggcggacgag agatttgctt
     1501 tgccgcgtac cgcgtcgatc tcgccgatca gtgccgccag cgacagaccg cgcgcactgg
     1561 cgatggcgcg caatttctcc cagaaagcct gttcgagtga gatgctggtg cggtgaccgg
     1621 cgatggtcag cgaatgtttg gagatcgcgg tcgcgtcccg atcaggcatc ttcggaacca
     1681 ggctcgcttt cgcggcgatg gccctcaagg cgccggcgct ccaactcgcg cgtagctgcg
     1741 gcgcgctcac gttcggcctt cggtgccccg aaccgcagcc ggttctccgc ggctttatcg
     1801 gctgcgtcgg cgcggccctt ggccttacga gcgcggcgca gattgacgat ctcactcatt
     1861 tttttcggaa ggcgtcgatg gacacgacct tcgtcggcga ttccgctggc gtgtcgtccg
     1921 actttgccag ttcgggcttt gtagatttgc ccaattcgga cttgctcaat tcaggtctgc
     1981 ccaacccttt gccaagcgat ggtttggccg cctcgcccag gtccgcctgt ttcggattga
     2041 tctccgctgg ttcgctgccg ctgccgcggg gcttggactt ttccggcgga acggaccgca
     2101 acgctggcgc ctgcggcgca atcgcgggct ggctcgtggc tgtgggggcc tcgttcgcca
     2161 tttcaaactt gaggccgaat tcgaccgagg gatcgaagaa gccggtgacc gccgtgaaag
     2221 gaatcaccag cagttccggc ttgcgatcga aatgcaggcg cacctcgaaa tgctgttcgg
     2281 tgaccgccag atcccagaat tcgtgctgaa ggacgacggt catttcctgc ggatattttt
     2341 cccgcagctt ggcaggtatc cgtacgccag gtgcgtcggt gcgaaagaca ataacgaaat
     2401 ggtggttgcc cggcaggccg tcgcgggccg cgtcggccaa caccttgcgc aaaaccgagc
     2461 gcagagcttc ctgcaccaga agatcgtagc ggatgagatc agacgccatt ccaatatccc
     2521 ggcccagata aagtggaggc ttctgttgcc aggtgcctcc gaaccccgcc ttaaagtgct
     2581 acccctaagg actttgaatc ggtaccatta ccgcattacg cggcaacggc caccggagca
     2641 tagttgtcgt tggcaactat aaaattagcc cgatgacggt ggaaccatgc cgagcaaaag
     2701 aacacccttt acactccccg tcgaacctat ttcgcccccg ccgaagctcg cctgatgacg
     2761 agctttggtg gaggcgccgg gtaccgcccc cgggtccgaa gagcttattg cgatgccgtt
     2821 tatcgccata tctgcgcaag gcagcgtcct gaatataggc agagacggaa taattagaaa
     2881 gtcccgcctc agcggccggc ctcgcgactt ggggatatga ccgcgtggcg actttcgcgg
     2941 gcggcccaag aatgcgatgc cgaggctggc gcgctaaagg actgaaggct gatggacgca
     3001 acgcccgaga tgcaaagcaa gcgcgcgccg tacggaatca ttgggctctg cctgagcatc
     3061 gtcgcggcaa tcgtccttac ccttatcgtc atggcggccg cggctgcggc ggtctatttc
     3121 ggcgacgctg ctctgcacgg catgggtgat gcgcggacaa aagcccgggc cgttctgtcg
     3181 actctgcaga tcgacgatca attcggcgcc gcgcggctgg cccttgggat attcttttac
     3241 gtcgcagcgc tggcgtcaat tctactggtc gcgtgctggc gggccgggcg ggattggcga
     3301 actctcgtcg cctggcgggt gccgctctgg cctatgcgtg acaaaatcct ttgggggatc
     3361 gttgcgatcg gcttgattta cgatttggca tcgagtgcag cactcgctca cttttacccg
     3421 cagtccaatt cctggcttca aatgccgcac ggccatatgg ccatcgccct gctcttggtc
     3481 gttgcggtca ttgtggcgcc cgtcgtcgaa gagacctatt ttcgcggctg gattttcacg
     3541 agcttgcgcc agagttgggg ccggtggccg gccatcatta tttcggcgct gctgtttggg
     3601 ctggcgcatt acgagtcgac gcatctttac gcgctcgcgg ttttcccgct cggcctcatc
     3661 ctcgcggcgc tgcgcgagcg aaccggaagt gcagggacgt cgatgctgtt tcacgcggca
     3721 aacaatttga tcgccgtctt atcggccgcc gcgagttcaa cctgaccatc tttcgagaca
     3781 cggaccaatg cgacatccag aaactcaata tctcgatctg ctggagcaaa tccttgaaca
     3841 tggagaccgg cgcgttgacc ggaccggcgt gggaacgctt tcgatctttg gcgcgctgac
     3901 gcgcttcagt ctcgccgacg gcaccgtgcc aatcctcacc actaagcgtg tttactggaa
     3961 gaccgcggtc aaggagatgc tgtggtttct gaccggcggt acgaatattc agccgctgct
     4021 caaaaacaac gtccatatct ggactgattg gccgctcgac cgttatcgca aggaaacggg
     4081 cggcgcgatc tcgcaatccg aattcgagca gaaaattatc gataacgatg tctttgccgc
     4141 gcgctggggc gggctggggc ctgtctacgg caaacaatgg cggcgatggc tcggaccgga
     4201 cggaaaagag cacgaccaga tcgcggcgct gatcaagacg attaagacaa acccgtccag
     4261 ccgccgcatg ttgttccacg cctggaacgt ggccgagatc gatcagatgt cgctaccgcc
     4321 gtgccacttg atttatcaat tctattgcac gcaggatggc aggctcag
//
LOCUS       DASWOG010000172         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_187477,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000172 DASWOG010000000
VERSION     DASWOG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187477"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB93_03450"
     CDS             <1..277
                     /locus_tag="VGB93_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0145208.1"
                     /translation="LVQAGPEGMQAGQIAAALGVPPNTLTFHFDRLRMAGLVSVRREG
                     RAMIYTAHFTTMNALLAYLAENCCGGVPCTPAQVCKPASDKRLKSPA"
     gene            297..815
                     /locus_tag="VGB93_03455"
     CDS             297..815
                     /locus_tag="VGB93_03455"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0145209.1"
                     /translation="MPDRIFNVLFLCTGNSARSILAEAILNEIGAGKFRAFSAGSHPK
                     GDINPNTLTLLKNLGHDVSSLCPKSWDEFALPGGPIFDFIFTVCDNAAGEACPVWLGH
                     PVTAHWGVPDPAEAKGTPAEIAQAFNEAYRMLHRRVELFALLPSRALDESSLQAKLKE
                     IGQTTDTKEAAG"
     gene            821..1504
                     /locus_tag="VGB93_03460"
     CDS             821..1504
                     /locus_tag="VGB93_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0145210.1"
                     /translation="MRLSQRLAAEWLGTAFLLTAIIGSGIMAERLAHGDGALALLCNS
                     LATGTMLAVLILIFGPISGAHFNPLVSLALFMRGELTVATAASYVVAQLAGAFAGVLA
                     AHAMFALPLMQVSMHARSGPGQCLSEAIATFGLLMTIFGCAARNPDATPYAIGAYIVA
                     AFWFTASTSFANPAVTLARAYSDTFAGIAPSNVPGFVLAQCVGTLAAIALAPWLWKTV
                     LAKPANSRT"
     gene            complement(1488..>2150)
                     /locus_tag="VGB93_03465"
     CDS             complement(1488..>2150)
                     /locus_tag="VGB93_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEY0145211.1"
                     /translation="PALEKKAKGGDKEAKETFDIVSRCLVLLREGKPARLAAIKPEER
                     KAFNMLGLLSAKPVLYVCNVEEASADKGNAYSEQVKVRAAQEGAGAVTVSAAIESEIA
                     LLPAAEQKDYLEAVGLEEPGLNRVIRAGYALLHLITYFTVGPKETRAWTIEVGTKAPQ
                     AAAVIHTDFEKGFIRAETIAYADYVAANGEAGAREAGKFRLEGKDYTVADGDVLHFRF
                     AN"
BASE COUNT          381 a          695 c          619 g          455 t
ORIGIN      
        1 gctggtgcag gcggggccgg aggggatgca agccgggcag attgcggcgg cgcttggcgt
       61 tccgcccaat acgctcacct ttcatttcga tcggttgcgc atggcgggcc tggtcagcgt
      121 tcggcgcgaa ggccgcgcga tgatctacac ggcgcatttc acgacgatga acgcgttgct
      181 cgcttatctc gccgagaatt gctgcggagg cgtgccttgc acgccggcgc aagtctgcaa
      241 gcccgcatcc gacaaacgtc tcaaatcccc cgcgtaacca gagatcgagc ttcacgatgc
      301 cagaccggat attcaatgtc ctgttcctct gcactggtaa ttcggcacgc tcgattctcg
      361 ctgaggcgat tctgaatgaa atcggcgctg gaaaatttcg cgccttcagc gcgggttctc
      421 atcccaaggg cgacatcaat ccgaacacct tgacgctcct gaaaaacctc ggccacgacg
      481 tttcgtcatt gtgcccaaaa tcatgggatg agttcgcttt gcccggcggt cccattttcg
      541 atttcatctt cacggtctgc gataatgctg cgggcgaagc ctgcccggtc tggctcggac
      601 atccggtcac cgcgcattgg ggcgtgcccg acccggcgga ggcaaaagga acgcccgccg
      661 agatcgccca agcctttaac gaagcctatc ggatgttgca ccggcgcgtc gaacttttcg
      721 ccttgctgcc ttctcgtgcg ctcgacgagt ccagtctgca agcgaagtta aaagagatcg
      781 gccagacgac cgacactaaa gaagccgccg ggtaaaactg atgcggcttt cgcagcgcct
      841 ggctgccgaa tggctgggga cagcctttct gcttacagcg atcatcggct cgggcatcat
      901 ggccgagcgt ttagctcacg gcgacggcgc actcgccttg ctctgcaata gccttgcaac
      961 aggtactatg ctggccgtcc tgatattgat ctttggccca atctccggcg cgcacttcaa
     1021 cccgctcgtc agcctcgccc ttttcatgcg cggcgaactg accgtcgcaa cagcggcaag
     1081 ctatgtcgtc gcacagcttg ccggcgcctt tgctggcgtc ctcgcggcgc atgccatgtt
     1141 cgccctgccg ctcatgcaag tgtcgatgca tgcacggtcc ggccccggcc aatgtctgag
     1201 cgaagcgatt gccaccttcg gtcttttgat gacgatcttc ggatgcgcgg cgcgaaaccc
     1261 ggacgcgacg ccttacgcga tcggcgccta tatcgtcgcg gccttttggt tcaccgcctc
     1321 gacatccttc gccaatcccg cagttacgct cgcccgcgct tattcagaca cgtttgcggg
     1381 gatcgcgccg tccaatgtcc ccggattcgt gctcgcacaa tgcgtcggca cactcgccgc
     1441 aatcgcactg gcgccttggc tctggaaaac agtgctcgcg aagccggcta attcgcgaac
     1501 ctgaaatgca gcacgtcgcc gtcggcgacg gtgtaatcct tgccttcaag acgaaacttg
     1561 cccgcctcgc gcgcgccggc ctcgccgttt gcggcgacgt aatccgcata ggcaatcgtt
     1621 tcagcgcgga tgaagccctt ctcgaaatcc gtatgaatca cggccgccgc ctgcggtgct
     1681 ttggtgccga cttcgatcgt ccaggcgcgg gtttctttgg ggccgaccgt gaaataggtg
     1741 atgagatgca ggagcgcata gccggcgcgg atgactcgat tgagccccgg ctcttccagc
     1801 ccgacggctt cgagataatc cttttgctcc gctgccggca gcagtgcgat ctcgctctcg
     1861 atcgcggcag agacggtgac agcgcccgcg ccttcttgcg ccgcgcggac tttcacctgc
     1921 tctgaatagg cattgccctt gtcggccgac gcttcctcga cattgcagac ataaagaacc
     1981 ggcttcgcac tcaggaggcc aagcatgtta aaggccttcc gctcctcggg cttgatcgcg
     2041 gcgagacgcg caggcttgcc ctcgcgcagc aagacgaggc agcggctgac gatatcgaag
     2101 gtttccttgg cctctttgtc gccgcccttg gctttcttct ccaaagcggg
//
LOCUS       DASWOG010000173         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_4195, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000173 DASWOG010000000
VERSION     DASWOG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGB93_03470"
     CDS             <1..442
                     /locus_tag="VGB93_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145212.1"
                     /translation="AFGVTLYERVARSELEGRVETALRGAALWEEVKDKLNASGLSLS
                     GGQQQRLCIARTVAIHPEVILFDEPCSALDPISTAKVEELIDELKDKYTIAIVTHNMQ
                     QAVRVSERTAFMYLGELVEFGLTHQIFTAPENQRTQNYVTGRFG"
     gene            470..1183
                     /gene="phoU"
                     /locus_tag="VGB93_03475"
     CDS             470..1183
                     /gene="phoU"
                     /locus_tag="VGB93_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0145213.1"
                     /translation="MTDHTVRAYDDELEALGRKIAEMGGIAEQMLSNAMDALATLDSD
                     LARVTISMDLRLDVLQRDVEEQAILTIAKRQPLAADLREIVAAIRISGDLERVGDLAK
                     NIAKRALKLSGETRLPRAIVGLKSMHEAAAMLLKDALDAYALRDVERARMVWTHDVEL
                     DALEDSVFRDLLTFMMEDPRNISFCAHLLFCSKNIERIGDHSTNIAETVVYLVTGESL
                     PMDRPKGPSPAADYLPMPK"
     gene            1191..>1759
                     /locus_tag="VGB93_03480"
     CDS             1191..>1759
                     /locus_tag="VGB93_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145214.1"
                     /translation="MNDQIVSVPDVRSQQDVRTAPPRVNSAAVAPPRILVVEDEAALS
                     LLLSYNLEAEGFIVERVERGDEAELRLVESPPDLVILDWMLPGVSGLEICRRMRARDA
                     TRSLPVIMLTARGDESERVRGLSIGADDYIVKPFSVPELMARVRALLRRARPDRVAAL
                     LIAGDLALDRENWRVSRGDRNVHLGPTEFR"
BASE COUNT          329 a          538 c          547 g          345 t
ORIGIN      
        1 tgccttcggc gtgacgcttt atgagcgcgt ggcgcgctcc gaactcgaag ggcgggtgga
       61 aaccgcgttg cgcggcgccg cgctctggga ggaagtcaag gacaagctca acgccagcgg
      121 cctgtcactc tcgggcggtc agcagcagcg cctgtgcatc gcccgcaccg tcgccattca
      181 cccggaagtc atcctgttcg acgagccctg ctcggccctc gatccgattt ccactgcgaa
      241 agtggaagaa ctcatcgacg agttgaaaga caagtacacg atcgcgatcg tcacgcacaa
      301 tatgcagcag gcggttcgcg tttccgaacg cacggccttc atgtatctcg gcgagctcgt
      361 cgagtttgga ctgacgcacc aaatcttcac cgcgcccgaa aaccagcgga cgcagaatta
      421 cgtcaccggc cgcttcggct gaggtttccg gtttcaggac cctattgcca tgaccgatca
      481 taccgtcaga gcctacgacg acgagctcga agcccttggc cgcaagatcg cggaaatggg
      541 cggcatcgcc gagcaaatgc tgagcaatgc gatggatgcg ctcgcgacgc ttgattccga
      601 tcttgcgcgc gtgacgatct caatggattt gcggctcgac gtgctgcaac gcgacgtgga
      661 agagcaggcg atcctgacga ttgccaagcg ccagccgctg gcggcggatt tgcgcgagat
      721 cgtcgcggcg atccgtattt ccggcgatct cgaacgcgtc ggcgatctgg ccaagaatat
      781 cgcgaagcgg gcgttgaagc tgtccggtga gacgcggctc ccccgcgcca tagtcggcct
      841 gaagtcgatg catgaggcgg ccgcgatgct gttgaaggac gcgctcgatg cttatgcact
      901 gcgcgatgtt gaacgcgcgc gcatggtctg gacgcacgat gtagaacttg atgcgctaga
      961 agattcggtt ttccgcgacc tgctgacgtt catgatggaa gacccgcgca atatctcgtt
     1021 ctgcgcgcat ctcctcttct gctccaagaa catcgagcgg attggcgacc attccaccaa
     1081 tatcgcggaa acagttgtct atttggtcac cggagaaagt ctgccgatgg atcggccgaa
     1141 aggcccctcg cccgcggccg attatctgcc catgccgaag tgagtcctag atgaacgatc
     1201 agatcgtctc agttccggat gtgcgctcgc agcaagacgt gcgaaccgcg ccgccccggg
     1261 tcaattccgc cgccgtcgcg ccgccgcgta ttcttgtcgt ggaggatgag gccgcgctca
     1321 gcctgttgtt gagctataat ctcgaagccg agggcttcat cgtcgagcgt gtcgagcgcg
     1381 gtgacgaagc ggagttgcgg ctggtcgagt cgccgcccga tctcgtcatc ctcgattgga
     1441 tgctgcctgg cgtctcaggg ctcgaaatct gccggcgcat gcgggcacgc gatgcaacgc
     1501 gcagccttcc agtcatcatg ctgacagcgc gcggcgacga gagcgagcgg gtgcgcggtc
     1561 tctcgatcgg cgcggacgat tatatcgtca agccattctc ggttcccgag ctgatggcgc
     1621 gcgtacgcgc cttactgcgc cgcgcccgtc cggatcgtgt cgcagcgctg cttattgcag
     1681 gcgatcttgc gctcgatcgg gaaaattggc gcgtcagccg cggcgatcgg aacgtccatc
     1741 ttggaccgac cgaattccg
//
LOCUS       DASWOG010000174         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_168945,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000174 DASWOG010000000
VERSION     DASWOG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168945"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VGB93_03485"
     CDS             <1..1023
                     /locus_tag="VGB93_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145215.1"
                     /translation="AEDGARQSLVTKNIVIATGSDVAPLPGLEIDEKQIVSSTGALAL
                     DKVPGKIVIVGAGVIGLELGSVWHRLGAEVLVVEFLDHILGPMDGEVGRQFQRLLEKQ
                     GFEFLLGHKVTKAEKSDAGVKVTIAPANGGDEIVRDADIVLVAIGRRPFIAGLGLEAA
                     GVATERGRVVIDEHFATNVPGIYAIGDVVRGPMLAHKAEDEGIAVAEIIAGQHGHVNY
                     EAIPSVVYTMPEIASVGATEEDLKAKGIAYNVGKFPFTANGRARANRQTEGFVKILAD
                     AATDRVLGAHIVGAGAGEAIAELCVLMEFGGSAEDLARTCHAHPTLSEAIREAALAVA
                     KRAIHI"
     gene            1107..1676
                     /locus_tag="VGB93_03490"
     CDS             1107..1676
                     /locus_tag="VGB93_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0145216.1"
                     /translation="MTFSPVDVTLFRTPAILLSGPVDYAMYNNFRFQLGNAPAEGIVV
                     VELSTLGGDPEVARMMGEDVRYHSDIAPNRRFVFLGKAAIYSAGTTFMSFFLPENRFL
                     TRGTRLMIHERKLAKTVNINGPLTTCVATVKALLHEIEASIIIQNEGFENLIRGSQVT
                     MEEVLEKTPNDWYIEASEAQRLGLIAAVV"
     gene            complement(1683..2327)
                     /locus_tag="VGB93_03495"
     CDS             complement(1683..2327)
                     /locus_tag="VGB93_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0145217.1"
                     /translation="MRGKFWSLIGEAAMGFLFASSLRADNVRNFSVTFVEAQSSSGVA
                     TALKSDARACLLSHVCDEAAALQELGRDRFVLYESYDASARPQQPALPVGALLAPPDT
                     RQLLEIWTSTASRMPPHALWVVTHVDAMPDHAAEVAIMLKQLGEANAREAGHLRTIVG
                     LQLDHPNHFTLIEVWTTRAAFEMHEAAKPTRDFRTKLAPMIGALYDSRLYQSVE"
     gene            complement(2391..2522)
                     /locus_tag="VGB93_03500"
     CDS             complement(2391..2522)
                     /locus_tag="VGB93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145218.1"
                     /translation="MTPYGAIEAHYSIGLGCEMRYAGSRRSLSGIFDDWAQFAVMAL"
     gene            2599..2765
                     /locus_tag="VGB93_03505"
                     /pseudo
     CDS             2599..2765
                     /locus_tag="VGB93_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493604.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            2999..3232
                     /gene="rpmE"
                     /locus_tag="VGB93_03510"
     CDS             2999..3232
                     /gene="rpmE"
                     /locus_tag="VGB93_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0145219.1"
                     /translation="MKSEGHPNYHFIKVVTTDGTEFYTRSTYAEEGATLNLDIDPKTH
                     PAWTGGSQQLLDRGGRLSRFNSRFGNLGIAAKK"
     gene            complement(3287..3808)
                     /locus_tag="VGB93_03515"
     CDS             complement(3287..3808)
                     /locus_tag="VGB93_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEY0145220.1"
                     /translation="MIQLSTSEQDAAVSFANKLVSSESFKSLFRDGMTLVEETAAYLD
                     GEGREDAKNLPRLGALAYATESMRLTTRLMQTASWLLLQRAVNEGELTQTEAASEKRK
                     VHLSEQQTGISADAAKKLPERFVAIIDRSLRLQSRILHLDRVLYQAADEAPVLSRPLE
                     PQLELLRAAFGGE"
BASE COUNT          843 a         1275 c         1278 g          784 t
ORIGIN      
        1 gcggaagatg gcgccagaca aagcctggtg acgaagaaca tcgtcatcgc cacaggctcc
       61 gatgtcgcgc ctttgcccgg tctggagatc gacgagaagc agatcgtctc ctcaaccggc
      121 gccctcgcgc tcgacaaggt gccgggcaag atcgtcattg tcggcgccgg cgtcatcggc
      181 ctcgaattgg gctcggtctg gcaccggctt ggcgccgaag tgctcgtggt cgaatttctg
      241 gatcacatcc tcgggccgat ggacggcgag gtcgggcgtc agttccagcg cctcctcgaa
      301 aagcagggtt tcgaattttt gctcggccac aaggtgacga aggccgaaaa gtccgatgcc
      361 ggtgtcaagg tgacgatcgc accggcgaat ggcggcgacg agatcgtccg cgatgcggat
      421 atcgttctcg tcgcgatagg ccgccgtccg tttatcgccg ggctggggct ggaagccgcc
      481 ggcgtcgcga cggagcgcgg ccgcgtcgtc atcgacgaac atttcgccac caatgtgccg
      541 ggcatctatg ccatcggcga tgtcgtgcgc gggccgatgc tggcgcataa ggccgaggac
      601 gaaggcatcg ccgttgccga gatcatcgcc ggtcagcatg ggcatgtgaa ctacgaggcg
      661 atcccctccg tggtctacac gatgccggag atcgcctcgg tcggtgcgac cgaagaagac
      721 ctgaaagcca agggcattgc ctacaatgtc ggcaaattcc ccttcactgc caacggccgc
      781 gcccgcgcca acaggcagac cgaaggcttc gtcaaaattc tcgccgatgc cgcgaccgac
      841 cgggttctcg gcgcgcatat tgtcggcgcc ggtgccggcg aggccatcgc cgaactttgc
      901 gtgctgatgg aattcggggg ctcggcggaa gatctggccc gcacctgcca cgcgcacccg
      961 acgctctccg aggcaatccg cgaagccgcg ctcgcggtgg ccaagcgcgc cattcatatt
     1021 tgacatcgcc gcgagcggaa cccgtcctcg cgccgcagca ttaacgcggc agcggtgtgg
     1081 ctcggctccg cttgaaaggg gattgcatga ccttctcgcc agtcgatgtc actttgtttc
     1141 gcacgcccgc gattttgctg tccgggccag tcgattacgc catgtacaat aatttccgct
     1201 ttcagctcgg caacgcgccg gcggaaggca tagtcgtcgt cgaactttca actttaggcg
     1261 gcgacccgga agtcgcgcga atgatgggcg aggacgtccg ctatcatagc gacatcgcgc
     1321 cgaaccgccg cttcgtcttt ctcggcaagg cagcgatcta ttccgccggc acgaccttca
     1381 tgagcttctt cctgccggaa aatcggtttc tgacgcgcgg cacgcggctg atgattcatg
     1441 agcgcaagct cgccaagacc gtcaacatca acggaccgct gacgacctgc gtcgcgaccg
     1501 tgaaggcgct gctgcacgaa atcgaagcct cgatcatcat ccagaacgag ggtttcgaga
     1561 atctgatccg cggctcccag gtgacgatgg aagaagtgct cgagaagacg cccaacgatt
     1621 ggtacatcga agcgtccgaa gctcaaaggc tcggcctcat cgccgcggtg gtctgagtcc
     1681 gctcactcga cggactggta gagccgtgaa tcataaagcg cgccaatcat cggcgcgagt
     1741 ttggtacgga aatctcgggt cggcttcgcc gcttcatgca tttcgaacgc agcgcgcgtg
     1801 gtccagactt cgatcagcgt gaaatggttg ggatgatcga gctgcagccc gacgattgtg
     1861 cgcagatggc ccgcctcgcg cgcgttggcc tcgccaagtt gttttaacat gattgcgacc
     1921 tctgccgcat gatccggcat ggcatcgaca tgcgtcacga cccatagcgc gtgcggaggc
     1981 attcgcgacg cggtgctggt ccaaatctca agcaattggc gtgtgtcggg aggcgccaag
     2041 agagccccga ccggtaacgc cggctgctgc gggcgcgcgg atgcatcgta agattcataa
     2101 aggacgaaac ggtctcggcc aagttcttgc aaagccgcag cttcgtcaca aacatgactc
     2161 aagaggcacg cgcgcgcgtc cgatttcaga gccgtcgcga cgccagacga agattgcgct
     2221 tcgacgaacg tgacggaaaa gtttcggacg ttgtccgcgc gcaggcttga cgcgaaaagg
     2281 aagcccatcg cggcctcgcc gataagactc cagaacttgc cacgcatgcc gcgccccgca
     2341 ttcaatcgaa ttgagtggcg gaattgcgcg ttccagtgaa gggactcaac tcacaaagcc
     2401 atgaccgcaa actgcgccca gtcatcaaag atcccggaaa gcgaacggcg tgatccggcg
     2461 taacgcatct cgcagccgag accgatggaa taatgcgcct cgatggcgcc atagggcgtc
     2521 acggctgggc acaccgtcca gaggcgaggg aaaccggccc gatgccagtc ttttcgagac
     2581 attgtcgacg ccatcgacgc gacccgccac cagcgcgaag tcggcaccgg ctatttcgac
     2641 gccgtggccg aagtcctctc gggtggcaag tcctctcggg tggcaagtcc tcgacgacgg
     2701 cgctggccga ttcgactgag gcggatcagt tcaaaagcgc caaaaagcag caggccgccg
     2761 agtaaggcca aaaagcttcg gctcacaagt tcgcttgtga gccgggcgtg aaaaggttat
     2821 aggggcgcct cgcttgcgcg ctttccggtc gcccggcacc gggcgaccga caagaaatcg
     2881 ccccacctaa ggtggagcaa attctggtcg aaaaagtcga gcaacttttt cggaattcgc
     2941 tcttggcgcc cccttatttt tgcggcctct gcgccgcctc aacatctgga ttttgacgat
     3001 gaaatcggaa ggtcatccga actatcactt catcaaggtc gtcacgaccg acgggacgga
     3061 attttacacc cgctcgacct atgccgagga aggcgcgacg ctgaacctcg acatcgatcc
     3121 gaagacgcat ccggcctgga ccggcggttc gcagcaattg ctcgaccgcg gcggccgtct
     3181 gtcgcgcttc aattcgcgtt tcggcaatct cggcatcgcc gccaagaagt aagcctgttc
     3241 gcgctttcgc ttttagaaaa tgccgtaagc ggcagagggg cgaagtttat tcgccgccga
     3301 aggctgcgcg cagaagctcg agctgcggct caagcggccg gctgagcacg ggcgcctcat
     3361 ccgcagcctg atagagcacg cgatcaaggt gcaggatgcg gctttgaagc cgcagcgaac
     3421 gatcgatgat cgcgacaaac cgttccggca atttcttcgc ggcatccgcg ctgatgccgg
     3481 tctgctgctc gctcaggtgg accttgcgct tttcagatgc cgcttcggtc tgggtcaatt
     3541 cgccttcgtt caccgcccgc tgcagcaaga gccaggaggc tgtctgcatc agccgggtcg
     3601 tcagccgcat actctcggtc gcataggcca gcgcgccgag acgcggcaaa ttcttggcgt
     3661 cttcgcgccc ctcaccatcg agataggccg cggtttcctc gacgagcgtc atgccgtcgc
     3721 ggaacaggct tttgaaggat tcagacgaca ccaatttatt ggcgaaggat acggcggcgt
     3781 cttgttcgct cgtcgacagc tggatcattg cggcgctctt acctaactct ttccgggcag
     3841 aattttccgc accgatatta ggaccttcgg cagggcgccg ggcattaagt tttaagtaag
     3901 gttaacgtgt gaagcatcgc aaaagccgcc gcgcccgcat gatgcagatg agaaaaagag
     3961 ccgccgaagc ggctctgaaa gtcacggtgt tggggaggga aatgccgcgg ccggggccgc
     4021 agttctgtcc ggcaagctca ccggctgact cttacgatgc gcgcgaaaaa tttaattaaa
     4081 gattagcgat ccccggcggc ccattcagga cgccggctta aaaaaggctg ccgcgctggc
     4141 ctggctggcg gccttggcgc gttgggcctc ttcgagcctg
//
LOCUS       DASWOG010000175         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_114160,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000175 DASWOG010000000
VERSION     DASWOG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114160"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VGB93_03520"
     CDS             <1..374
                     /locus_tag="VGB93_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0145221.1"
                     /translation="LLAIVPIDSAYVLANYQETQLTPLALGQKVEITVDALPGVVLTG
                     HVDSLAPASGIAFSPIAPDNATGNFTKVVQRIPLKIVFDPDQPLRARLRVGMSVETTL
                     DTHSTPDKNDINRALDHLATR"
     gene            371..2035
                     /locus_tag="VGB93_03525"
     CDS             371..2035
                     /locus_tag="VGB93_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0145222.1"
                     /translation="MSVRGSLPVWEYVLRVGPLCRDAGVGALVLALAGCAVGPDWYKP
                     APVMPQVFARPEGPKAPSRPIAAPVDPAWWRIFGDSELAQLEARVAQDNFDIREAATR
                     YAESRAEREMAASAFYPSSDVDASYANERASPNGVLNLLGTTATNTPATIANGSPGFG
                     PAGITSADTGNASSPAFNLWQYGIDASWEVDIWGRVRRSIEAADAGVELSEDYRRGVL
                     ISVLAETAGDYIALRGVQAQIAITRENLGVARHSLALTRLRFTNGATTNLDVANATAD
                     VAAVGARLPPLERQESELINALSFMVAAPPRELAGALRPAKGLPPIPRKVPVGLPSEL
                     AEKRPDIRAAAAQLHEATADIGVAIGDFYPRITLEGSLDIQSLEFSHLGLWNSRQYGL
                     GPVISLPIFEGGRLTGNLELKEEQQKRAALDYRRTVLKAWHEVDDALTAYNAAQNERN
                     LLATAVAQDKIALAAAQLQYSQGATDFLNVLTVQNRLLDAQNAEIAAAIGADAALISL
                     YKALGGGWEANELPLRAPQPRLPVVALLPHPIALKQTPDVDLPHGP"
     gene            2063..2773
                     /locus_tag="VGB93_03530"
     CDS             2063..2773
                     /locus_tag="VGB93_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0145223.1"
                     /translation="MIEKSILVEPQCPQVARLSLVDSAIVIIRSQVETGAWKVGERIP
                     KEDELAEMLRVGRNTVREAVRVLSHANMLEVRQGDGTYVRSNVDPGEMLRRVDRASLH
                     DHFELRATLETDVARLAATRRTKADIEDMTRLLDERGEIPPYGSTDFKAFVARDLAFH
                     LAIAHAAHNAAMEELYAYFLKYEQQHLDAYFGRGDVIEPNGPAHARVLEAIIARNPVR
                     AARAARAILAPVIKTLKG"
     gene            complement(2854..3101)
                     /locus_tag="VGB93_03535"
                     /pseudo
     CDS             complement(2854..3101)
                     /locus_tag="VGB93_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3473..>4642
                     /locus_tag="VGB93_03540"
     CDS             3473..>4642
                     /locus_tag="VGB93_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145224.1"
                     /translation="MAFAKNGSGATVRAIASEWLAVASVALGTFTLVTSEFLPVGLLT
                     KVAASLHTSEGATGLMVTTPGVVAAIAAPAVMMGAGKLDRRIILWILSAILVISNLTV
                     TLAPNLPVLLLGRVMLGIDVGAFWAISSVIATKMVAPSSVSRANAIIFAGISVGTVVG
                     VPAGSLIGDTFGWRAAFGAIAAFGALVLIAQLLFLKRLPPSEIVSLRHLTALFGIPKA
                     RLGLLATMMAIIAQFGAYTYMGAFLEQVTLAPAALLSALLLGYGIAGFFGNFVGGAAV
                     AHNARWTLAGTALLSGASIVLLPAIGWLEAPAVVLVILWGFAFGALPIAMQGWMLQAA
                     PGEMESASAMFISVLQVGLASGSLLGGVVVDHFGLDVALVSSGCVALITAGLVWFF"
BASE COUNT          857 a         1426 c         1421 g          938 t
ORIGIN      
        1 cactcctcgc aattgtgccg atcgattcgg cctatgtgct cgcgaactat caggagacgc
       61 agctcacccc tttggcgctc ggccagaagg tggaaatcac agtcgatgcc ttgccgggcg
      121 tcgtgctgac tggccacgtc gacagtctcg cgccggcgag cggcatcgcg ttttcaccga
      181 tcgcccccga caatgcgacc ggcaatttca ccaaggtcgt gcagcgcatt ccgctgaaga
      241 tcgtcttcga tccagaccag cctttacggg cgcggctacg ggtcggcatg tcggtcgaga
      301 cgacgctcga tacgcacagc acaccggaca agaatgacat caatcgcgcg ctcgaccatc
      361 tggcgacgcg atgagcgttc gggggagtct tcctgtgtgg gaatatgtat tacgcgtcgg
      421 cccgctgtgt cgcgacgcag gggtcggcgc acttgtgctg gcgctcgcgg ggtgtgccgt
      481 gggtccggac tggtacaagc ccgcgccggt gatgccgcag gtcttcgcca ggcccgaagg
      541 accaaaggcg ccgagccggc caatcgcggc gccagtcgat cccgcctggt ggcgcatctt
      601 tggcgattcg gagcttgcgc agctcgaagc gcgcgtggcg caggacaatt tcgacattcg
      661 cgaagccgca acgcgctatg cagaaagccg cgccgagcgg gagatggcgg cttccgcctt
      721 ctatccgtcc tccgacgtcg acgcgtccta tgcgaacgag cgggcgagcc ccaacggcgt
      781 tctgaacctg ctcggcacga cggcgacgaa cacgccggcg accattgcca atggctcacc
      841 cggcttcggc ccggcgggaa tcacctcggc ggacacaggc aacgcgtctt cgccagcctt
      901 taacctctgg caatatggga tcgacgcgag ctgggaggtc gacatctggg gccgggtacg
      961 ccgctcgatc gaggccgccg acgccggggt tgaactctcg gaggattacc ggcgcggcgt
     1021 gctgatcagc gtgctggccg agacggccgg cgattacatc gccttgcgcg gcgttcaggc
     1081 gcagatcgcc atcacgcgcg aaaatctcgg cgtcgcaagg cacagcctgg cgttgaccag
     1141 gctgcggttc accaacggcg cgacgaccaa tcttgacgtt gccaacgcca cggctgatgt
     1201 cgccgccgtc ggggcgaggc tgccgccgct cgagcgccag gaatccgagc ttatcaatgc
     1261 gttgagcttc atggtcgcgg cgcccccgcg ggagctcgcc ggcgccttgc ggccggctaa
     1321 ggggttgccc cctattccgc ggaaagtgcc cgtcggcctg ccctccgaac tcgccgagaa
     1381 gcgccctgac attcgcgccg cagcggcgca attgcacgag gcgacggcgg atatcggcgt
     1441 cgccatcggc gatttctacc cgcgcatcac gctggaaggc agcctcgaca tccagtcgct
     1501 cgaattctcc catctcgggc tctggaattc gcgccaatac ggcctggggc ccgtcatcag
     1561 cctgccgatt ttcgaaggcg gccggctgac cggcaatctc gaactaaagg aagaacagca
     1621 gaagcgagcg gcgctcgatt atcggcgcac cgtgctgaag gcctggcacg aagtcgacga
     1681 tgcgctgacg gcttacaacg cggcgcagaa cgagcgcaat cttctggcga ccgccgtggc
     1741 gcaggacaag atcgcactcg cggccgcgca attgcaatat tcgcaaggtg cgacagactt
     1801 cctcaacgtg cttaccgtcc agaaccgtct gctcgacgcg cagaacgccg aaatagctgc
     1861 ggccattggc gccgatgcgg ctttgatcag cctctacaaa gcattgggcg gcggctggga
     1921 ggcgaacgag ctgccgcttc gagcgccaca gccaaggctt ccggtcgtgg cgttgcttcc
     1981 acatccgatt gcgctcaaac agacccctga cgttgatctg ccgcacggtc catgacaatt
     2041 gccgtgagag gaatgaattc gcatgattga aaaatcgatt ctcgtcgaac cgcaatgtcc
     2101 gcaagtcgcg cggctctcgc tcgtcgactc ggcaatcgtc atcatccgtt cgcaagtcga
     2161 gaccggtgca tggaaagtcg gcgagcgtat ccccaaggaa gacgaactgg ccgagatgct
     2221 tcgcgtcggc cgcaacacgg tccgtgaggc ggtgcgcgtg ctctcccatg ccaatatgct
     2281 ggaagtgcgc caaggcgacg ggacgtacgt ccgctccaac gtcgatcctg gcgaaatgtt
     2341 gcgccgtgtc gatcgcgcga gtctgcatga ccatttcgaa ctgcgcgcga cactcgaaac
     2401 cgacgtcgcc cgtctcgccg ccacgcgccg caccaaggcc gatatagagg acatgacccg
     2461 cctgctcgac gaacgcggcg aaatacctcc ttatggcagc acagatttta aggccttcgt
     2521 cgcccgcgat cttgccttcc atctcgcgat cgcccacgcc gcgcataatg ccgcgatgga
     2581 ggaattgtac gcttacttcc tcaaatacga acagcagcac ctcgacgctt atttcggccg
     2641 cggcgatgtc atcgaaccca acggtccggc gcatgcccgc gttctcgaag ctatcatcgc
     2701 gcgcaatccg gtgcgagccg cgcgtgccgc acgcgcgatt ctggccccgg tcatcaagac
     2761 gctgaaaggc tagtgtgagc ggcatagtta tcgatgacca gtgaatatcg aggatcttgg
     2821 gtacgttggc atcgatgtgt ttgaaaaagc ccaaaacacc tgatgacgat ggcgcgcacg
     2881 gcattgggtc atgacctgcc gttagcgatg ctcagggcgg cgaacagtgt ggtagtccca
     2941 tggcggctgt aatcatgcat gaggccttcg atataaccga ggccgagcgg caggatgggt
     3001 tgggtgcgtt caagcgcctg gatttgcatc ttttcgtcaa cgcacaacac aaccgcatga
     3061 tcaggcggat tgaggttgaa cccgacgatg tctttgacct tgagtgcgca cgggtgatcc
     3121 gttccaaatc ctcgcgctcg ctgcccacca ccgtcgatac acactcctgg ggtctaccaa
     3181 tgcgcgctat gatccgctcc cctcaatgcc gggcggttca tgaatctgtt cgtgccttat
     3241 gtaaatttat ttgcgggagg gaatacctag gtctccatcg atctgggcat cagaggaata
     3301 ccgggtcgga gtagctcaac gccgggatta tcccgacttt ttgatttgaa tttgtattgc
     3361 tcgctacata atagggttga cgagcgccat tcggctctga aactgactgt gcgcgggtct
     3421 tcgaatagcg cggcgtcagg cctcgcccac ttctcactca aaggattcaa gaatggcttt
     3481 tgccaagaat ggcagcggcg cgacagttcg ggcgatcgcc tcggagtggc tcgccgtggc
     3541 ttcggtggcc ctcggtacat tcacgctcgt gacaagcgaa ttcctgccag tgggattact
     3601 gaccaaggtc gcagcttcgc ttcatacctc agaaggcgca accggtctga tggtgacaac
     3661 gccaggcgtc gtcgcggcga tcgccgcacc ggcagtgatg atgggtgcgg gtaagctcga
     3721 ccggcggatc atcctatgga tattgagtgc catactggtg atatcaaatc taaccgtcac
     3781 gctagcgcct aatctgcctg tcttgctgtt aggccgcgtc atgctaggca ttgatgtcgg
     3841 tgccttctgg gccatcagca gtgtgatcgc cacaaagatg gtggctccat cgtcggtcag
     3901 ccgcgctaac gcaatcatct tcgcgggaat ttctgtcggc actgtcgtgg gcgtaccggc
     3961 cggctcgctg attggcgata ccttcggctg gcgcgccgcc ttcggtgcta tcgccgcctt
     4021 cggcgcattg gtcctgatcg cgcagttgct attcctcaaa cgcctgccac cctccgagat
     4081 cgtgagtctt cggcatctga cagccttgtt cggcataccc aaggcgcggc tcggtctgtt
     4141 ggcgacgatg atggcgatca tcgcgcaatt cggcgcgtac acctatatgg gggcgttcct
     4201 cgaacaggtg actttggcgc cggcagcatt gctctcggcg ctgctgctgg gctacggcat
     4261 cgccggcttc ttcggtaatt tcgtaggggg cgccgcggtc gcacacaatg cacggtggac
     4321 gctggccgga actgcactgc tgtcaggtgc gtcgatcgtc ctactgcctg ctatcggttg
     4381 gctagaggca ccagctgtcg tgctggtcat cttatgggga tttgctttcg gagcgctgcc
     4441 gatcgcaatg cagggctgga tgctgcaagc agcgcctggc gagatggaaa gcgcgagcgc
     4501 catgttcatc tcggtacttc aggtcggtct cgcctcggga tctctcctag gtggagtcgt
     4561 ggtcgaccat tttggcttag acgtcgcgct cgtcagttct ggttgtgtgg cactcattac
     4621 agctggtcta gtttggtttt tc
//
LOCUS       DASWOG010000176         8196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_168992,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000176 DASWOG010000000
VERSION     DASWOG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168992"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1232)
                     /gene="fabB"
                     /locus_tag="VGB93_03545"
     CDS             complement(9..1232)
                     /gene="fabB"
                     /locus_tag="VGB93_03545"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY0145225.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVLASLREARSGIVRADKYAELGF
                     RSQVHGAPTLDPEPMIDRRAMRFHGGGTAWNHVAMDQAILDAGLSPAEISNERTGVIM
                     GSGGPSTRTLIEAADIARTKGPKKIGPFAVPKAMSSTASATLATWFKIRGLNYSISSA
                     CATSNHCIGNAYEAIRYGKLDRMFAGGCEELDWTLTVLFDAMGALSSSYNDRPEVASR
                     AYDKNRDGFVISAGGGVLVLEEFEAAKARGAKIYAEIAGYGVTSDGADMVAPSGEGAA
                     RCMKMALQDVKVPIDYINPHATSTPIGDLKEIEAIRAVFGNEDKCPPISATKSLTGHA
                     QGATSVHEAIYSLLMMQAGFICKSAHIEELDPAFADMNILRERRDNVSLRAVLSNSFG
                     FGGTNASLVFKHLDA"
     gene            complement(1296..1823)
                     /gene="fabA"
                     /locus_tag="VGB93_03550"
     CDS             complement(1296..1823)
                     /gene="fabA"
                     /locus_tag="VGB93_03550"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEY0145226.1"
                     /translation="MSERRSSFDYEDLIACGRGDLFGPGNPQLPLPPMLMFDRIAEIS
                     DHGGANGKGTIRAEFSIKPDQWFFNCHFKGDPVMPGCLGLDALWQLLGFYLGWLGLQG
                     RGRALGVGEVKFTEQVLPTVKRLIYGVDLKRVFNTKLVLGIADGWLEADGRRIYEATD
                     LKVGLFKAATPSSAA"
     gene            complement(2065..2544)
                     /locus_tag="VGB93_03555"
     CDS             complement(2065..2544)
                     /locus_tag="VGB93_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0145227.1"
                     /translation="MPAAAEAAAPNGQTIGSVSGLPVPRFVSLKADKTNLREGPSKDH
                     RTTWVYQRAGLPVEITAEFGMWRKVRDSDGTEGWVLHNLLSGRRTAIVAPWKKDSIFT
                     LYIRPAEDSAPVARLQPGVIGNIKICDGDWCEIYGEGFDGFIRQNNLWGVYPSEKVD"
     gene            2878..3873
                     /locus_tag="VGB93_03560"
     CDS             2878..3873
                     /locus_tag="VGB93_03560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY0145228.1"
                     /translation="MSRKKPLVVLTRKLPDVVETRMRELFDARLNVDDKPMSHDDLVA
                     AMRSADVLVPTVTDRIDAELIEKAGSQLKLIANFGNGVDNIDVGAAFQRGIAVTNTPG
                     VLTQDTADMTMALILAVARRIVEGAEVIPEGNWTGWSPTWMLGTRITGKRLGIVGMGR
                     IGQALAYRARAFGLQIHYHNRRHVAAAIEEDLEATYWDSLDQMLARMDIVSVNCPRTP
                     ATFHLLSARRLQHLRPQAILVNTARGEIIDEKALIEMLEAGELAGAGLDVFEHEPAVS
                     ERLVKLAKAGKVTILPHMGSATREGRIDMGEKVIINVKMFMDGHRPPDRVLPSAV"
     gene            4018..4446
                     /locus_tag="VGB93_03565"
     CDS             4018..4446
                     /locus_tag="VGB93_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0145229.1"
                     /translation="MSRMLTINSPFMLGFEDFERMVDRVTRAGSEGYPPDNIERVAKT
                     DAEPERLCITLAVAGFMREQLEVTVEEGQLVIRGRQQDEKPRDFLHRGIAARQFQRVF
                     LLAEGMRVANCDLAHGLLTVDLVRPEPIRVVKKLDIMMRD"
     gene            4495..4767
                     /locus_tag="VGB93_03570"
     CDS             4495..4767
                     /locus_tag="VGB93_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HEY0145230.1"
                     /translation="MLDEFKEVEETVTPLTEEQFAHLGGGSLAYVRAISSEDVARLFP
                     QAPALQPGLQLFALLGADGTPIMLTDSRDAALGNALQQELQTVSVH"
     gene            complement(4834..5100)
                     /locus_tag="VGB93_03575"
     CDS             complement(4834..5100)
                     /locus_tag="VGB93_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145231.1"
                     /translation="MRTKTQIEKVSGRHVSSQPGDAPEVALIAILVAMVLLRILDVYS
                     PPASATAGCAPTHASRAVISCAVRGDQTWDASSTQAAASLSGRR"
     gene            complement(5219..7387)
                     /locus_tag="VGB93_03580"
     CDS             complement(5219..7387)
                     /locus_tag="VGB93_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY0145232.1"
                     /translation="MAKDCTGERREPTFDAFPEDAPRRRPPKKRKSKKRPRRSIVNRL
                     VYWVLMLGVWGAVGASLVVAYYAAQLPPIDQLTIPKRPPNIAILAEDGTLLANRGDTG
                     GAAVHLAELPAYLPKAFVAIEDRRFYDHWGIDPIGILRALVRDALHRGGMEGGSTLTQ
                     QLAKNLFLTQERTLPRKIQEAILAVWLEHKYSKDQILELYLNRVYFGAGAFGVEAAAQ
                     KYFGHSARNASLAEAAMLAGLMKAPSRYAPNHNLAAATQRADEVIAAMRDQGFITPAM
                     AQLALDEPAQPAKPKAGGTINYVADYVMDQLDDLVGPVDEDISVTTTIAAPLQAAAEK
                     ALRETLAAKGAHYDVSQGAVVALDPGGALRALVGGRDYNESQFNRATTAKRQPGSAFK
                     PFVYLTALEHGMTPDTIRNDAPIYLRGWHPEDYEHRYMGPVTLTTALANSLNTVAVRL
                     ALEVGPVNVARTARRLGITSNLDANPTIALGTSVVTPLELVSAYVPFANGGISVTPYV
                     VTKVTAAHGKLLYFHQPLAGNRVIAPEYVAMMNRMMAETLATGTARRVDLPGWPAAGK
                     TGTSQDFRDAWFVGYTSHMVCGVWLGNDDNSPTKRASGGSLPVVVWSQFMQVAHADVA
                     PAPLTDGVWTGPTLAEPVASATNAIVNFFTGQTPPTQAPRAAWPQQPSQSWPRAAQTQ
                     SAPYRPARREGEDDGLPPASIPSSGHEASQQPARSFFGLF"
     gene            7677..>8196
                     /locus_tag="VGB93_03585"
     CDS             7677..>8196
                     /locus_tag="VGB93_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0145233.1"
                     /translation="MQVTLERAALLKALGHVHRVVERRTTIPILSNILFSAEGSSVLL
                     KATDLDLEVTDRLPADVTQAGGTTLPAHILYEIVRKLPDGAQVSLDVTGEATQLLLRS
                     GRSRFHLQALPESDFPDLAAGDFTHRFSLSATDLKKLIEKTQFAISNEETRYYLNGIY
                     LHTAEADGKTVLR"
BASE COUNT         1526 a         2497 c         2625 g         1548 t
ORIGIN      
        1 ggcgctgctc aggcatcgag atgcttgaag acgagcgagg cgttggtgcc gccgaagccg
       61 aaggaattgg agagcacggc gcgcaggctt acattgtcgc ggcgctcgcg caggatgttc
      121 atgtcggcga aggccgggtc gagttcctcg atatgggcgc tcttgcagat gaagccggcc
      181 tgcatcatca gcagcgaata gatcgcctca tgcacgctcg tcgcgccctg ggcgtggccg
      241 gtcagtgact tggtggccga gatcggcggg cacttgtcct cgttgccgaa gacggcgcgg
      301 atcgcctcga tctccttcag gtcgccgatc ggcgtcgaag tcgcatgcgg gttgatatag
      361 tcgatcggca ctttgacgtc ctgcaaagcc atcttcatgc agcgcgccgc gccctcgccc
      421 gagggggcga ccatgtcggc gccgtcagag gtcacgccat agccggcgat ctcggcatag
      481 attttcgcgc cgcgcgcctt ggcggcttcg aattcctcga gcaccagcac gccgccgccg
      541 gcggaaatga cgaaaccgtc gcggttcttg tcataggcgc gggaggcgac ctccggccga
      601 tcattatatg aagacgagag cgcgcccatc gcgtcgaaga ggacagtcag cgtccagtca
      661 agttcttcgc agccgccggc gaacatgcgg tccagcttgc cgtaacggat cgcttcgtag
      721 gcattgccga tgcaatggtt cgaggtcgcg caggctgagg agatggaata gttcaggccg
      781 cggatcttga accatgtggc gagcgttgcc gacgcggtcg aggacatggc ctttggcacc
      841 gcgaaggggc cgatcttctt cgggcccttg gtgcgggcga tatccgcggc ctcgatcagc
      901 gtgcgggtcg aagggccacc cgaccccatg atgacgccgg tgcgctcgtt ggagatttcg
      961 gccggcgaca aaccggcgtc gaggatcgct tgatccatcg cgacatgatt ccaggcggtg
     1021 ccgccgccgt gaaagcgcat ggcgcgccgg tcgatcatcg gctcggggtc gagcgtcggg
     1081 gcgccatgca cttggctgcg aaagccaagc tcggcgtatt tatcggctct gacgatgcca
     1141 gatctcgcct cgcgcaagga cgcgagcacc tcttgggtgt tgttgccaat tgaagagacg
     1201 atgcccatgc cggttacgac gacgcgtctc attacaatct cctgcctcgg gccgcctcag
     1261 cggcctttaa accgtctgtg cgaattcagt tgggtttagg ctgcggatga tggagtcgcg
     1321 gctttgaaaa ggccgacttt caagtccgtt gcttcgtaga tacggcgtcc gtcggcttcc
     1381 agccagccgt cggcgatgcc gagcactaat ttggtgttga acacgcgctt cagatcaact
     1441 ccgtagatca atcttttcac ggtgggcaga acctgttcgg tgaatttcac ctcgccgacg
     1501 ccaagcgccc ggccgcggcc ctgcaagccg agccagccga gatagaagcc gagcaattgc
     1561 cacagcgcat cgaggccgag acagcctggc atcaccgggt cgcccttgaa gtgacaattg
     1621 aagaaccatt gatccggctt gatcgagaat tccgcgcgga tcgtgccctt gccattggcg
     1681 ccgccgtgat cggagatctc agcgatcctg tcgaacatca gcatgggcgg taggggcaat
     1741 tgcggattgc cgggaccaaa caggtcgccg cgtccgcagg caatgaggtc ttcgtaatcg
     1801 aagctggagc gtcgctcgct cattctgtct ttcaggttcg agaggtcgcc ccgaaggcgc
     1861 acctacgcga gcgaagatgg cggcttctaa catagccccc gccgcgctga aaagggtccc
     1921 gatcaagctc tttgccgagc gccgaccgct gactctatat atattgcaac gcggtaaaca
     1981 tcgcaccgag atgcagggcg agggccgtga ttaagttttg ttaatccagc ggaatcccgg
     2041 aaagccggag tgatggcgtt cgctttagtc gactttttcg ctcggataga ctccccagag
     2101 attgttctgg cggataaagc cgtcaaatcc ctcgccgtag atttcgcacc agtcgccatc
     2161 acagatcttg atattgccga tcacgcccgg ctgcaggcgg gcgacgggcg ccgaatcctc
     2221 ggccggccgg atataaagtg tgaaaatact gtccttcttc cagggcgcga cgatggccgt
     2281 gcgccgcccg gacagcaaat tgtgcaaaac ccagccttcc gtgccatccg aatcgcgaac
     2341 cttgcgccac atcccgaatt ccgcggtgat ctccaccggc agaccggcgc gctggtagac
     2401 ccaggtggtg cggtggtcct tagaagggcc ttcgcgcaag ttggttttgt cagccttgag
     2461 actgacgaag cgcggcacgg gcaggccact gacgctgcca atcgtctgtc cgttaggagc
     2521 ggcggcctcc gcggcggccg gcatctggcc gtgcagccac ccggagaggg caagcccagc
     2581 cacgaccaat gcacgtgtga agataatatg gcccttcatg cccgggaatt tcgtcgccaa
     2641 atccgcgtcc actttactca ccagaggccg gcgatgccga cgtttttatg tcgccgcgcg
     2701 ttgcgcttgg cattcttgtg tttggaccgc catctgctag agaggcgcgg aaccttgccg
     2761 cgcgaccttg cccgaattgg tcgccatatt aagtgcgttg cgctgagtta aggccgcgtg
     2821 aaagcctggc cccagggcct gcctccggcg gcggacggac gggataacgg gattcagatg
     2881 tccaggaaaa aaccgctcgt agtcctcaca cgcaaactgc cggatgtggt cgagacgcgg
     2941 atgcgcgagc ttttcgacgc ccgcctcaat gtcgatgaca agccgatgag ccatgacgat
     3001 cttgtcgcag cgatgcgcag cgccgatgtg ctggttccga ccgtgaccga tcgaatcgat
     3061 gccgaactca tcgaaaaagc cggcagccag ctcaagctca tcgccaattt cggcaatggc
     3121 gtggacaata tcgatgtcgg cgccgccttt cagcgcggca tcgccgtcac caacacgccg
     3181 ggcgtcctga cgcaggacac ggccgacatg acgatggcgc tgattctcgc cgtcgcgcgg
     3241 cgcatcgtcg aaggtgccga agtcattccg gaaggcaatt ggaccggctg gtcgccgacc
     3301 tggatgctcg gcacgcgaat caccggcaag cggctcggca ttgtcggcat gggccgtatt
     3361 ggccaggcgc tcgcctatcg ggcgcgggcc ttcggcctgc aaatccatta tcacaaccgc
     3421 cgccacgtcg ccgctgcgat cgaggaggat ctcgaagcga cctattggga ctcgctcgac
     3481 cagatgctgg cacggatgga tatcgtttcc gtcaattgcc cgcgcacgcc ggcgaccttc
     3541 catctgctct ccgcgcggcg actgcagcac ttgcggccgc aggcgatcct ggtcaacacg
     3601 gcgcgcggcg aaatcatcga cgagaaggcg ctgatcgaga tgctggaggc gggcgagctc
     3661 gccggcgcgg ggctcgacgt cttcgagcac gaaccggcgg tgtccgagcg tctggtcaag
     3721 ctagccaaag ccggcaaggt gacgatcctg ccgcatatgg gctcggcgac gcgcgagggc
     3781 cggatcgata tgggcgagaa ggtcatcatc aacgtcaaga tgttcatgga cggtcatcga
     3841 ccgcccgatc gggttttgcc gagcgcggtc tagcctccga cagcgctaag cctatgcttt
     3901 gacacccttc tgagtcctcg cgcccacgcc cttgaagggc cgccgctaat ctccatattt
     3961 ccctcagcgg aggtgccaca ctgggtctcc caacacgaag gcctgagggc ttcgctcatg
     4021 tcacgcatgt tgaccatcaa ctcgccgttt atgctcggct tcgaagactt cgagcggatg
     4081 gttgaccggg tgacgcgggc gggttccgag ggctatccgc ccgacaacat cgagcgggtc
     4141 gcaaagaccg atgcggagcc ggagaggtta tgcatcaccc tggccgtggc cgggttcatg
     4201 cgcgagcagc tcgaagtgac ggtcgaggaa ggccaattgg tcatccgggg ccgccagcag
     4261 gacgaaaagc cccgcgattt tctccaccgg gggattgcgg ctcgccagtt ccagcgggtt
     4321 ttccttcttg ccgaagggat gcgcgttgca aactgcgatc tggctcatgg attgctgacg
     4381 gtggatctcg tccggccgga gccgatacga gtggttaaga agctcgatat catgatgcga
     4441 gactgaatgt tcgcatccat gcgggtctcg aagagaccgc agaaggagtg tagaatgttg
     4501 gatgaattca aagaggttga agagaccgtg acgccgttga ccgaagagca gtttgcccat
     4561 ctgggcggcg gttccctcgc ctatgtgcgg gcgatcagtt cagaggacgt cgcacggctt
     4621 ttcccgcagg caccggcgct ccagcccggg cttcagttat ttgctttgct gggcgccgac
     4681 ggcacgccga tcatgctgac ggattcacgc gacgccgctt taggcaacgc gctccagcaa
     4741 gagctgcaga cggtcagcgt tcactaagcc caaattgaaa atcgctcgcg catggatgcc
     4801 tgcgcgagcg atctcgcaat gaagcggcaa tggctagcgg cggccgctga ggctcgccgc
     4861 ggcttgcgtg gatgaagcat cccaagtctg atcgccacga accgcacagg atatgacggc
     4921 gcgactggcg tgcgtcgggg cgcagccggc agtcgcggac gctggcggcg aatagacatc
     4981 aaggattcgc agcaggacca tcgccaccaa aatcgcgatc agcgcgacct cgggagcatc
     5041 gcccggttgg ctcgaaacat gccgccccga gaccttttca atctgcgttt ttgttctcat
     5101 ttaaatctcc gaagccgaga attggctgcg ggctttcact tggcgggaag cgcataaaaa
     5161 atccatgcgt cttgatgggc ttggatatta aagccgtcgg atagctgccc cgaaattctc
     5221 aaaaaagtcc gaaaaacgac cgtgccggct gttggctcgc ttcatggccg gaactcggaa
     5281 tcgaggccgg cggcaggccg tcatcctcgc cttcgcgacg tgccgggcga tacggcgcgc
     5341 tttgggtttg ggccgcccgc ggccaggatt gagaaggttg ctgcggccag gcggcgcgcg
     5401 gggcctgtgt cggcggagtt tgcccggtga agaaattcac gattgcgttc gtggcgctcg
     5461 caacgggctc cgcgagggtc ggcccggtcc agacaccgtc cgtcagcggc gctggcgcga
     5521 catcagcatg ggcgacctgc atgaactgcg accagacgac gaccggcaac gaaccgccgg
     5581 aagcgcgttt ggtcggggaa ttgtcgtcgt ttccgagcca cacgccgcaa accatatggc
     5641 ttgtgtagcc gacgaaccac gcatcgcgaa aatcctggct ggtgccggtt ttgccggctg
     5701 cgggccagcc tggcaggtcg acacggcgcg ccgtgcccgt ggcaagcgtc tcggccatca
     5761 tccgattcat catcgccaca tattccggcg cgattacacg gttgcccgcc agcggctgat
     5821 gaaaatagag cagctttccg tgcgccgccg taaccttggt gacgacatag ggcgtcacgc
     5881 tgatgccgcc gttggcgaac ggcacatagg cggagacgag ttcaagcggc gtgacgaccg
     5941 atgtgccaag cgcgatcgtc ggattggcgt cgaggttcga ggtgatgccg agccggcgcg
     6001 cggtccgcgc gacattgacc ggcccgactt cgagggcgag ccgaaccgcg accgtattca
     6061 gtgaattggc gagcgccgtc gtcagcgtca ccgggcccat gtagcgatgt tcgtaatctt
     6121 ccggatgcca gccgcgcaga taaatcggcg catcgttgcg gatcgtgtct ggcgtcatcc
     6181 cgtgttccaa cgccgtcaga tagacgaagg gcttgaacgc cgagcctggc tgacgtttgg
     6241 ccgtggtcgc gcggttgaat tggctttcgt tatagtcacg accgccgacg agcgcacgca
     6301 aggcgccgcc cggatcgagc gcgacgaccg cgccctgact cacatcgtaa tgcgcaccct
     6361 tggcggcgag tgtttcgcgc aaggctttct ccgccgcggc ttgaagcggc gcggcaatcg
     6421 tcgtggtgac ggaaatgtct tcgtcgacgg gcccgaccag atcgtcgagc tgatccatga
     6481 cgtaatcggc gacgtaattg atggtgccgc cagctttggg cttcgccggt tgggccggtt
     6541 catcgagcgc caattgcgcc atggccggcg tgataaagcc ttggtcgcgc atcgccgcga
     6601 tcacttcgtc cgcgcgttgg gtcgcggccg cgaggttatg gttgggcgcg tagcgcgagg
     6661 gcgccttcat cagccccgcg agcatggccg cctcggcgag gctcgcgttg cgcgcgctat
     6721 ggccgaaata tttctgtgcc gcggcttcga caccaaaagc gccagcgccg aaatagacgc
     6781 ggttcagata gagttcgagg atctggtcct tggaatattt gtgctccagc cagacggcga
     6841 ggatggcctc ctggattttc cgcggcagcg ttcgttcctg tgtcagaaag aggttcttgg
     6901 cgagttgctg ggtgagcgtc gaaccgcctt ccatgccgcc gcgatggagc gcgtcgcgga
     6961 ccagggcacg caggatgcca attgggtcga tgccccaatg gtcgtaaaag cgccggtcct
     7021 cgattgcgac gaacgccttc ggcagatagg caggcaattc ggcgagatga acggccgcac
     7081 cgccagtgtc gccgcgattg gcgagcaacg tcccgtcctc ggcgaggatg gcgatgttgg
     7141 gcggccgctt ggggatcgtg agctgatcga taggcggcaa ttgcgcggcg taataggcca
     7201 cgacaagcga ggcgccaacg gcgccccaga cgccaagcat cagcacccaa taaacaagtc
     7261 gattgacgat cgaccggcgc ggacgcttct tcgacttgcg ctttttgggc ggccggcggc
     7321 gcggcgcgtc ctcaggaaag gcgtcgaagg tcggttcccg ccgttccccg gtgcagtcct
     7381 tggccatggc tctcacgcgc ccgcttatcg gatgcccgcc gcgtaggctc tgccggcgga
     7441 cggcgcgatc ctagacgggt gcgattaagg cgcggtaaaa tggcgaacgg ccggagacgg
     7501 ggcggcgaaa cggctgcgcc tctgcctagg cggcggccaa gctcgaaaac ggggataacc
     7561 gggtgtagcg gcgcttgcga tttggcttga gccgcgccac agttacgccc ccgcgcggcc
     7621 tccactgccg cctgccagcc gattgaccag ctcgctcaaa agtccggatt cgtctcatgc
     7681 aagtcactct cgaacgcgcc gctctgctca aggcgctcgg ccacgtgcac cgcgtcgtcg
     7741 aacggcgcac gacgattccg atcctgtcca acattctctt cagtgccgaa ggcagttcgg
     7801 ttttgctcaa ggcgaccgat ctcgatctcg aagtcaccga ccggctgccg gcggatgtga
     7861 ctcaggcggg cggcaccacg ctgccggcgc atattctcta cgagatcgtg cgcaaactgc
     7921 cggacggcgc gcaggtttcg ctcgatgtga cgggcgaggc gacgcaattg cttttacgtt
     7981 cgggccgctc gcgttttcat ctgcaagcgc tcccggaaag cgacttcccc gatcttgccg
     8041 cgggcgactt cactcaccgt ttcagcctgt cggccaccga tctcaagaag ctgatcgaga
     8101 agacccagtt cgcgatctcc aacgaggaaa cgcgctatta tctgaacggc atctatctgc
     8161 acactgccga ggccgacgga aagaccgtgc tgcggg
//
LOCUS       DASWOG010000177         7184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_132521,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000177 DASWOG010000000
VERSION     DASWOG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132521"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VGB93_03590"
     CDS             complement(<1..445)
                     /locus_tag="VGB93_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0145234.1"
                     /translation="MDYLAAVRAFVRAVDLGSFSKAAVEQELKVSTVSRYVSGLEADL
                     GVALLNRSTRRLHLTEGGADFYRRAVAILAEVEEARLAMHTLNASPRGVLRVNIPSAF
                     GRRHIVPHLKDFHDLHPQVRLDISLTDTVVNLIETGTDVAIRIGAL"
     gene            603..1013
                     /locus_tag="VGB93_03595"
     CDS             603..1013
                     /locus_tag="VGB93_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEY0145235.1"
                     /translation="MLAAIFILLAAELLGELLRSALHVSVPGPVIGMIALATWLIWRG
                     QTTQPEPSALARVADGILGHLGLLFVPAGVGIIGELAVIQHEWLPILGGVIGSTVLSL
                     AVTGLVLHHLLRRKATPKRPAVLSMGDNACIAKS"
     gene            995..1714
                     /locus_tag="VGB93_03600"
     CDS             995..1714
                     /locus_tag="VGB93_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY0145236.1"
                     /translation="MYREILAVWTPLAPSPLVWIVLTIGAYLAGCWLQRCAGGAAYAN
                     PVLFAVVVVGLVVVATGTSYSTYFGGAQFINFLLGPATVALAVPLARNFHHIRRNLAS
                     IGLALAAGSATAIVSGIVIVHMLGGSRAIALSMAPKSVTTPIAMAVSTEIGGDPALTA
                     ALAMVGGILAAIVGEKMLKGLRVADWRAHGLAAGVAGSGVAAAQAASLDGLAAAFAAL
                     GIALNGLMTALILPLVPLVWH"
     gene            complement(1748..2704)
                     /locus_tag="VGB93_03605"
     CDS             complement(1748..2704)
                     /locus_tag="VGB93_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0145237.1"
                     /translation="MPPADRHGPSVDPARTFDPVPMFSAPGHRDTIWSDMMGSVARST
                     QITWAGGTAATHRINKVSGAAEIRTRCLELQFSWNSGYVEADADRGKQIHAYKNRPCY
                     GLLLPPETGVEFRIKEKSNYRFLSIELEPEYVLRVSELQHLSSVEFLETWNYEHPLTW
                     QLARAISDECETEARAGLLYCETVVALLALHVTRHLSNYSRALVASHRGGLAPGVLRR
                     ACEYMTSRLADDLSLSEIADIGGLSPGHFSFAFKRSTGMSPHAWLRRERIDRAKALLR
                     DRSLSLPFIASAVGFSTQSAFGVAFKRETGLTPTAWRRAHWL"
     gene            3102..3926
                     /locus_tag="VGB93_03610"
     CDS             3102..3926
                     /locus_tag="VGB93_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0145238.1"
                     /translation="MQQFARVQTDKGIIPVILKGDAHLDLRPIISDITPDAIASGALA
                     QVDTSRLAPISDSFTYLAPIAGIRQIAATGFNYKKHIEEFKMKPPTEPEVFLKAVTSL
                     TGPSDPISRGPNQGVKLDWETELTIVIGREAKDITAAEAPDYIFGYVCLNDVSDRATQ
                     VDAEGGQHLVRAKSRPTYTPIGPYLATGIDGMNVDVWTKLNGHYEQQGNTSDMLFDIN
                     EMVAYFSTHMVLLPGDLIATGTPPGVGFGKNRFMAVGDVLECGISHLGAQRHEIIA"
     gene            4021..4605
                     /locus_tag="VGB93_03615"
     CDS             4021..4605
                     /locus_tag="VGB93_03615"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY0145239.1"
                     /translation="MGRPNLKDKILTAAVDRLTAKGYGATSVQDITDAASAPKGSFYN
                     HFRSKEHLAVEALGAYQTSVRPERLQDNALPPLERLRNHFEFMISLLEAKEFRHGCLM
                     GGLAAFVDNEQPLVQQAVGAAYADMTADIAAVLGEAKGLGKLPSSENVSELAGVIFDA
                     WEGALLRARVEGRRRPLDAFVSVIFDQLIPGLAR"
     gene            4725..6149
                     /locus_tag="VGB93_03620"
     CDS             4725..6149
                     /locus_tag="VGB93_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0145240.1"
                     /translation="MLYETINPATGKVVQTFPAITDSELESALAKADALYREDWRHRA
                     VADRARILSKAAALLLQNAEDYARTITEDMGKLLSVAVQEVHLSASILDYYAKHAATF
                     LEPKHFPGFPGAALVARPIGAILAIEPWNFPFYQVARVAGPQLMAGNVLLVKHAESVP
                     QSALALERLLKEAGAPEGAYTNIFANHDQISRLIADPRIVGVTLTGSERAGAAVGEQA
                     GRHLKKVVLELGGSDPLIVLPDAPLDHAVGSALFGRMFNCGQSCVASKRIIVVGKERG
                     ATFIDNFVAKARAFKVGDPTDAATELPPLSSERALTGLLSQIEAARAGGAKVVTGGRR
                     VDRPGFYLEPTVITGIDEKNPVYSQELFGPVASIYVVENEEDAIRIANDSPYGLGGSV
                     FGADLKHARGVAERIESGMVYVNQPLWPAAELPFGGVKNSGFGREQSELGFGEFVNHK
                     LINVAPAEAPVWGPVEFEASQAAE"
     gene            6462..6920
                     /locus_tag="VGB93_03625"
     CDS             6462..6920
                     /locus_tag="VGB93_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0145241.1"
                     /translation="MGIGKGSKPPSSNMQTAVDADTGLIIYHEVTNEPNDTRQLYPMA
                     KATKNMLGVESLAVVADAGYSNGTAAAACEADGITACAPSNRTINTQGDGMMFDRVAF
                     TYRPEEDVYICPAGHVLARKFCATLSSSMRRGTAPAAHSSRDVPTQSGAS"
     gene            6845..7174
                     /locus_tag="VGB93_03630"
     CDS             6845..7174
                     /locus_tag="VGB93_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0145242.1"
                     /translation="MLYAARDCASCSLKPRCTNAERRFVTRHLHEDALERMNARFQAD
                     PSLIRQRRCASEHPFGTIKRMTAGGRFLARGLKKVSGEAALSVLAYNIIRTINLVGAA
                     FLRAKLA"
BASE COUNT         1417 a         2193 c         2161 g         1413 t
ORIGIN      
        1 ccagcgcgcc aatgcgaatc gcgacatcgg taccggtctc gatcagattc acgaccgtgt
       61 cggtcaacga gatgtcgagc cgcacctgcg gatgcagatc gtgaaaatcc ttgagatgcg
      121 gaacgatatg ccggcgcccg aaggcgctgg ggatgttgac gcgcaatacg ccacgtgggc
      181 tggcgttgag cgtatgcatg gccaggcgcg cttcctcgac ctcggcgagg atcgcgaccg
      241 cacggcgata gaaatcggcc ccgccttccg tcagatgcag ccgccgtgtc gagcggttga
      301 ggagcgcgac gccgaggtcg gcctcaagcc cgctgacata gcgtgaaacc gtcgagactt
      361 tcagctcctg ctccaccgcc gccttggaga aacttccaag atcgaccgcg cgcacgaaag
      421 cccgcaccgc agcaaggtag tccatagatc tctgtccgtc tgcgcaacaa tggtggcctt
      481 gtccgatatc ctatcaagct tttcgccgac attatgccct tttatgcaaa tgagatttga
      541 acgctgagcc gtttcacgct gcggtgcgag agagcatgtt ttcgctagca gggaggtttg
      601 ccatgttagc cgcgatcttc attcttctcg ccgcagagct tctaggcgaa ttgttgcgta
      661 gcgctttgca tgtgtcggtg cccggcccgg tgatcggcat gatcgcgctc gccacgtggc
      721 tcatttggcg ggggcagacc acgcagcccg aaccgagcgc gctcgcccgc gtggccgacg
      781 gcattctcgg ccacctgggt ctgctcttcg ttccagcagg tgtcggcatt atcggcgaac
      841 ttgccgtcat ccagcatgaa tggctgccga tcctcggcgg tgtcatcggc tcgacagtct
      901 tgagtctcgc cgtcacgggg ctcgtgctgc atcacctgct tcgccgcaag gcgaccccga
      961 aacgcccggc tgttctctcg atgggagaca acgcatgtat cgcgaaatcc tagccgtctg
     1021 gacgccgctc gcgccctcac cgctcgtctg gatcgtgctg acgatcggcg cctatctcgc
     1081 gggctgctgg ctgcagcggt gcgccggcgg cgcagcctat gccaacccgg tgttattcgc
     1141 tgtcgtggtc gtcggccttg tcgtcgttgc gacaggcacg tcctattcga cctatttcgg
     1201 cggcgcgcag ttcatcaact tcctgcttgg cccggcgacc gtggctctcg cagtgccgct
     1261 ggcgcggaat tttcatcaca tccgccgcaa cctggcgagc attggtctgg cgctcgccgc
     1321 cggatcggcg accgcaatcg tcagtggaat tgtcattgtt cacatgctgg gcggcagccg
     1381 cgccattgcc ctttctatgg cgcccaaatc cgtgacgacg ccgatcgcca tggccgtctc
     1441 gactgaaatc ggtggcgacc cggcgctgac ggcggcgctc gccatggtcg gcggtattct
     1501 cgcggccatc gtcggcgaga aaatgctgaa aggtctgcgc gtggccgatt ggcgtgcgca
     1561 cggacttgcg gccggcgtcg ccgggagcgg tgtggcggcg gcgcaggccg cgagcctcga
     1621 cggtttggcc gccgcgtttg ccgccctcgg catcgcgctg aatggtctga tgacggcgct
     1681 gattttgcca ctcgtgccgc tggtttggca ttagggctcg cggaaaaccg cgaacacgtg
     1741 gtttctgtca tagccaatgt gctcggcgcc aggctgtggg tgtcagccca gtctctcttt
     1801 tgaaggcgac gccgaacgcg ctttgcgtgg agaacccgac ggctgacgct atgaacggca
     1861 agctcaggct gcggtcacga agcaacgctt ttgcccggtc gattctctca cgacgcagcc
     1921 aggcatgcgg tgacattcca gttgatcttt tgaaagcaaa agagaaatgt ccgggcgaaa
     1981 ggccgccgat gtccgcgatc tccgaaagcg aaaggtcgtc cgccaagcgg cttgtcatat
     2041 attcgcacgc ccggcgaaga acgcccgggg ccaagccccc acggtgagaa gccacgagtg
     2101 cacgcgaata atttgaaaga tgtcgcgtga catgtaatgc cagaagcgcg acgaccgtct
     2161 cgcaataaag cagtcctgcc cgcgcttcag tttcacattc gtccgaaatc gcccgcgcca
     2221 gttgccaggt cagcggatgt tcgtaattcc aggtctcaag gaattcgaca gaagagagat
     2281 gctgcaactc agagacgcga agaacgtatt ccggctctaa ttctatggac aggaaacggt
     2341 aattggattt ttccttaatt ctgaattcga cgcctgtctc cggcggaagg agaagcccgt
     2401 aacaagggcg atttttatag gcgtgtatct gtttgcctct gtccgcgtcc gcctcaacgt
     2461 aaccagaatt ccatgagaat tgaagctcca ggcatcgcgt acgaatttcg gcagcgccgg
     2521 ataccttatt gattctatgc gtggcggccg tgccgccagc ccaagtaatt tgggtcgagc
     2581 gagcgacact tcccatcata tcggaccaga ttgtgtctcg gtgtcccggc gcgctgaaca
     2641 tcgggacggg gtcaaacgtt cgcgcaggat cgacggaggg gccgtggcga tctgctggtg
     2701 gcatcgcagc ctcctaggtc ttcgaagcta tttctgcagc gaaatcaaat tgatccgcag
     2761 ggaaaaagaa tcttcgtccc gtggcgaacc gcgaggggca gagctgggaa caattcgcaa
     2821 taggccattc tccagtcgtg acggcagaat tgcgttttca ccgttttgtc atcggaccag
     2881 tatttttagt ctcaaatcac ggcgcttgtc gagaaagtac gacaaagtcg cgcctgcgct
     2941 tttttttcca gtagagaaat ctagttcgat gtgctgtgca atgaacacgg ctacgaacga
     3001 cgtcgctgaa acataaccca cagtcgttcg ctccgcttcc acggcaaaaa aggttcgtcc
     3061 tttcaccgtt gcctgaaaac aaaattggag agcagcacgc catgcagcag ttcgcacgcg
     3121 tacagacgga caaaggcatc atccccgtca ttttgaaagg cgatgcacat ctggacttac
     3181 ggccgatcat ctccgacatc acgccggacg cgatcgccag tggtgcgctg gcccaggtcg
     3241 atacgtcccg cctcgcgcca attagcgatt ccttcacgta tctcgcgccg atcgctggga
     3301 taaggcagat cgcggcgacg ggcttcaatt acaagaaaca catcgaagaa ttcaaaatga
     3361 agccgccgac cgagccggaa gtattcctga aagctgtcac ctcgttgacc gggccgtccg
     3421 atccgatctc gcggggcccc aatcaaggcg tgaagctcga ttgggaaacg gaactgacca
     3481 ttgttatcgg gcgtgaggcc aaggatatca ccgcggccga agcgcccgat tacattttcg
     3541 gctatgtctg tctgaacgac gtttcggacc gggcgacgca ggtcgatgcg gaaggcgggc
     3601 agcacttggt gcgcgccaag tcgcggccaa cctacacccc catcggtcct tatctggcaa
     3661 ccggcatcga cgggatgaat gtcgatgtct ggaccaaact caacggccat tacgagcagc
     3721 agggcaatac cagcgacatg ctgttcgaca tcaatgaaat ggtcgcctat ttttcgaccc
     3781 atatggtttt gctgccaggc gatctgatcg cgacgggcac gccgccgggc gtcggcttcg
     3841 gcaagaaccg ctttatggcg gtcggtgacg tgctcgaatg cggcatctct catctcggcg
     3901 cgcaacggca cgagatcatt gcatagtgaa gtgtagagca gtctggcgct tgccgccgga
     3961 ctggacctgg accgtcccct cacgtatata tgaccgttcg tcttaccgaa cagagtgaag
     4021 atgggacggc cgaacctcaa ggataaaatt ctcaccgccg cggtcgatcg cctgaccgcg
     4081 aagggttacg gcgcgaccag cgttcaggac attaccgatg ccgcgagcgc gccaaagggc
     4141 tcgttctaca atcacttccg cagcaaggaa catctcgcgg tcgaggccct cggtgcttat
     4201 cagacgagcg tccgtccgga gcggctgcaa gacaacgcac tcccgccgct cgagcggctg
     4261 cgcaatcatt tcgagttcat gatctcgttg cttgaggcaa aggaatttcg ccatggttgc
     4321 ctgatggggg ggctggcggc attcgtcgat aacgagcagc ctcttgttca gcaggccgtc
     4381 ggcgccgcct atgcggacat gacggccgat atcgccgccg tgcttggcga ggccaagggc
     4441 ctgggcaagc tgccgagcag cgagaacgtc agcgaattgg ccggggtgat tttcgatgcc
     4501 tgggaaggcg cgctcctgcg cgcccgagtc gagggccgcc ggcgccccct cgacgccttt
     4561 gtctctgtga tcttcgacca gctcattccc ggcctggcgc ggtagaatct tccggtcgcg
     4621 cgccgctgat gtcgtcgccc ttgtctaaga cgaccggtcg tcgtaacatg gacggccaaa
     4681 gacggtgcga gctacggctc ggcaacaaag aggacatccc cccgatgctc tatgagacga
     4741 ttaaccccgc gaccgggaag gtcgttcaga cctttccggc aattacggat agcgaactcg
     4801 agtccgccct cgccaaagcc gacgcgctct acagggaaga ctggcgccac cgtgccgtcg
     4861 cggatcgcgc ccgtatctta tcgaaagcag cggccctgct tctgcaaaac gccgaggact
     4921 atgcgcgcac catcaccgag gacatgggca agctcctatc cgtcgccgta caggaagtgc
     4981 atctctcggc cagcatcctc gactattacg ccaagcacgc tgcgacattc ctggagccga
     5041 aacattttcc gggctttccc ggtgccgccc tggtcgcgcg cccgatcggc gcgatcctcg
     5101 ccatcgagcc gtggaacttt ccattttatc aggtcgcgcg tgtcgccggc ccccagctta
     5161 tggcgggtaa tgtgctgctt gtaaaacatg ctgaaagcgt gccgcaaagc gcgctcgcgt
     5221 tagagcgact tctaaaggag gccggtgcgc cggagggtgc ctacaccaac atctttgcca
     5281 accacgacca gatcagccgg ctgatcgcgg atccgaggat cgtcggcgtc accctgactg
     5341 gaagcgagcg cgctggcgcc gcggtcggtg aacaggccgg acggcatctc aagaaggtcg
     5401 tcctggaact gggcggcagc gatccgctga tcgtgctgcc cgatgcgccg ctcgaccacg
     5461 ccgtcggcag cgccttgttc gggcgcatgt tcaattgcgg ccaaagctgc gttgcgtcca
     5521 agcgcatcat cgtcgtcggc aaagagcgcg gtgcgacatt catcgacaat ttcgtcgcga
     5581 aggcgcgagc tttcaaagtc ggcgacccga ccgacgccgc gacggaattg ccgccgttat
     5641 cttcggaacg ggcgctgacc ggcctgcttt cgcagatcga ggcggcgcgc gctggcggcg
     5701 caaaagtcgt gaccggcggc aggcgcgtcg atcggcctgg cttctatctc gaaccgacgg
     5761 tgatcaccgg tatcgacgaa aagaatcccg tctacagtca ggagcttttc ggcccggtgg
     5821 cgtcgattta tgtcgtcgag aacgaagaag acgcgatccg cattgccaac gactctccct
     5881 acggtctcgg cgggtccgtg ttcggggccg atctcaaaca cgctcgcggc gtcgccgagc
     5941 ggatcgagag cgggatggtc tatgtgaatc agccactctg gcccgcggcg gagctgccgt
     6001 ttggtggcgt caagaattcc ggtttcgggc gcgagcaatc tgaactgggc ttcggtgagt
     6061 tcgtcaatca caagctcatc aatgtcgcgc cggccgaagc gccggtgtgg gggccggtgg
     6121 aattcgaagc ctctcaagcc gccgaatagg ccgcgccgca aattgctaag gccgcccatt
     6181 gcaggctgtg tgagaactct ggcgagtcag ctatcctggg ccgcatcgac aatggtgcgg
     6241 cctttttcct gagctagaca gcccgtctcg atcgcaggat tgccgaatat ctggccgctc
     6301 tagatgagaa cgatgcgcat gaacccgccg aaacaccgaa cgcgacggcc gcagcacttg
     6361 ctgccctcag agaacggcgc gccgagcttg atcgcctggc cgccagactc gatgccgaag
     6421 ggcctaccac cttggtcgaa ggcgagttgg atgcccgggc aatggggatc ggcaagggct
     6481 ctaagccgcc gtcctccaac atgcagacag ccgtcgacgc cgataccggc ctcattatct
     6541 atcacgaggt caccaacgag ccgaatgaca cgcgccagct ttatccgatg gccaaggcga
     6601 caaagaacat gctcggcgtc gagagcttgg cggtcgttgc cgatgccggc tactcgaatg
     6661 gaacggcagc cgcagcgtgt gaggctgatg gcataaccgc ctgcgcaccg agcaatcgga
     6721 cgatcaatac ccagggcgac ggaatgatgt tcgatcgggt ggcctttacc taccggccag
     6781 aagaggacgt ctatatctgt ccggcgggtc atgttcttgc ccgcaagttc tgcgcgacgc
     6841 tctcatcctc tatgcggcga gggactgcgc cagctgctca ctcaagccgc gatgtaccaa
     6901 cgcagagcgg cgcttcgtga cgcgtcattt gcacgaggac gcattggaac ggatgaacgc
     6961 ccgcttccag gctgatccga gcctaatccg acaacgccgg tgcgcctccg agcatccttt
     7021 cggaaccatc aaacgaatga ccgccggcgg ccgattcctc gcccgcggcc ttaagaaagt
     7081 cagcggggaa gcagcgctga gcgtgttggc ctacaacatc atccggacga tcaatctcgt
     7141 cggtgctgct ttcctgcgcg cgaagctcgc ctgagccaag caaa
//
LOCUS       DASWOG010000178         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_40467,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000178 DASWOG010000000
VERSION     DASWOG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40467"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1040
                     /locus_tag="VGB93_03635"
     CDS             81..1040
                     /locus_tag="VGB93_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0145243.1"
                     /translation="MRDPYIVLGVAKTASLDEIKKAYRKLAKKFHPDQSKDPKAKDKF
                     AEVSSAYEIIGDETKRGQFDRGEIDAEGKPKFQGFEGFGAGAGPGGFTRRAPGGGFEH
                     FEFRGAPGGAAGAGGFDASDIFSELFGAGRRGAGQQNRPLPRGEDVTADINVSLREAV
                     HGGKSRVSLPTGRTLEINVPAGIEDGKTIRLKGQGHPSAYGGEAGDALVTVHIAKHPY
                     FRVDNRDLRLDLPLTLYEAVLGAKVNVPTLDGKVELTVPPGSNGGRTLRLRGKGLPNP
                     GGTPGDLLVTLRIVLPDGSDPDLTALAEKWQKQHPYDPRQDLT"
     gene            1276..>1997
                     /locus_tag="VGB93_03640"
     CDS             1276..>1997
                     /locus_tag="VGB93_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145244.1"
                     /translation="MTQPERLSAETNATGILAGKRGLVLGAANNRSIAWGIAKAARAA
                     GAELAFTYQGESLEKRVRPLAEELQAAVVGHCDVTDEASIDAVFGEIERLWGSLDFVV
                     HCVAFSDKDQLTGRYVETTADNFSNTLLISCYSFTAIAQRAEKLMTNGGSLLTLTYYG
                     AEKRMPHYNVMGVAKAALEASVRYLAVVLGQKNIRVNATSAGPIKTLAASGIGDFRYS
                     LKGDEYNAPLRRSVTIDEVGESA"
BASE COUNT          391 a          590 c          649 g          367 t
ORIGIN      
        1 attggcgcgc tttcttatcg atcagatatc ccctccggtc ggaaagcgcg ctgcgggcgg
       61 gcgcgttaac gggctcgaag atgcgtgatc catatattgt cttaggtgta gcgaagacgg
      121 ccagcctcga tgaaatcaag aaggcctatc ggaagctcgc caagaaattt catcccgatc
      181 agagcaagga ccccaaggcg aaggataagt tcgccgaggt gagttccgcc tatgaaatta
      241 tcggtgacga aacaaagcgc ggccagttcg accgcggcga aatcgacgcc gaaggcaagc
      301 caaaatttca gggtttcgaa ggtttcggcg ctggcgccgg gccgggcggg tttacccgcc
      361 gcgcgccggg tggtggattc gagcactttg aatttcgcgg cgcaccgggt ggcgccgccg
      421 gagccggagg gttcgacgcg agcgacattt tcagcgaatt gttcggcgcg gggcgccgtg
      481 gcgccggtca gcagaaccgc cctttgccac gcggcgagga tgtgacggcg gacatcaatg
      541 tcagcctgcg cgaggcggtg catggcggca aaagccgtgt cagcctgccc accggccgca
      601 cgctcgagat caatgtccca gccgggattg aagacggcaa aacgatccgc ctgaaggggc
      661 agggccatcc gagcgcctat ggcggcgagg cgggcgatgc gctggtgacc gtccacatcg
      721 ccaagcatcc ctatttccgc gtcgataatc gcgatctgcg gctcgatctg ccgctgaccc
      781 tctacgaagc cgtgttgggc gcaaaggtca acgtaccgac gcttgacggt aaggtggaac
      841 tgaccgttcc gcccgggtcg aacggcggcc gcacgttgcg gcttcgtggc aagggtctgc
      901 ccaatccggg gggcacgccg ggcgatctgc tggtgacttt gcggattgtc ctgccagacg
      961 ggtccgatcc cgatctcacc gccctggcgg agaaatggca gaagcagcat ccctacgatc
     1021 cccggcagga tctgacctga accggccacc tggatgagat cacgattgcg ggctttgact
     1081 gtaacaatcg caagccatcg tgatttattg gccgtgaacc gcctgcaatc gcagcaagtt
     1141 ccgctttgaa aggcgtgggt ttgccgggat ggcgcgacga catgggcttg gccaatcggc
     1201 gtaaaccggg ttaaaggctt caagcccggc ggcgtcgccc gcgcgcggcc tctcaattat
     1261 tgcccaggac ccagaatgac gcagccggag cgcctatccg cagagacgaa cgccaccgga
     1321 attttggctg gtaaacgcgg cctcgtgctg ggcgcggcca acaatcgttc gattgcctgg
     1381 ggcattgcca aggcggctcg tgcggcgggc gccgagcttg ccttcaccta tcaaggcgag
     1441 tcgctcgaaa agcgcgtgcg cccgctcgcg gaggaattgc aggccgccgt cgtcggccat
     1501 tgcgacgtga ccgacgaagc gagcatcgat gcggttttcg gtgagatcga gcggctctgg
     1561 ggtagccttg atttcgtcgt ccattgcgtg gcgttttccg acaaggatca gctcacgggc
     1621 cgctatgtcg agacaacggc agacaatttc agcaacacgc tgctgatctc ctgctattcc
     1681 ttcaccgcga tcgcgcagcg cgccgaaaag ctgatgacga atggcggctc gctgctgacg
     1741 ctcacttatt acggcgccga gaagaggatg ccgcattata acgtgatggg cgttgccaag
     1801 gcggctcttg aagcctccgt gcgctatctc gccgtcgtcc ttggacagaa gaacattcgc
     1861 gtcaatgcga cttcggctgg accgatcaag acgctcgccg cgtcgggcat cggcgacttc
     1921 cgctacagcc tcaaggggga tgaatataac gcgccgctgc ggcgctcggt gacgatcgat
     1981 gaagtcggtg agagcgc
//
LOCUS       DASWOG010000179         7354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_205585,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000179 DASWOG010000000
VERSION     DASWOG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205585"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGB93_03645"
     CDS             complement(<1..339)
                     /locus_tag="VGB93_03645"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598848.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0145245.1"
                     /translation="MASLDSFKCRKILNVGAKTYHYYSLKTAEKNGLTGISSLPFSMK
                     VLLENLLRFEDGRSVTKESIQSVAEWLVNKGKVEKEIAFRQSRVLMQNFTGIPAVVNH
                     TAMPNAMLKLG"
     gene            469..1062
                     /gene="ccmA"
                     /locus_tag="VGB93_03650"
     CDS             469..1062
                     /gene="ccmA"
                     /locus_tag="VGB93_03650"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY0145246.1"
                     /translation="MQIEASALSVERGGRIIFAGLDFRLVAGEALLVKGPNGAGKSTL
                     LRAFAGFLPLAAGAISITPETEARRAELCHYIGHADALKPALTLAENLAFYTALLGGG
                     GIERALARLGLTGIADLPAGYLSAGQKRRAALARLVAIARPIWLLDEPLTALDARSQT
                     VVGELMQEHLAGGGCILAATHTPLPVAARDLMLGRPV"
     gene            1059..1742
                     /gene="ccmB"
                     /locus_tag="VGB93_03655"
     CDS             1059..1742
                     /gene="ccmB"
                     /locus_tag="VGB93_03655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY0145247.1"
                     /translation="MTESQQVRPSALGALLTRELRVGRRIGGAVSMGVVFFLCLVTVT
                     PFAVGPDQALLARIGPAILWIAALLATLLGLDRLFQADAEDGSLDLFLTSETPLELIV
                     LVKCLAHWLLTGLPLVAASPIFGLMLGLGPDGLVRVALSLLAGTPALSLLGAIGAALT
                     VSLRRGGLLLAVLILPLSVPVLIFGVAAAGDPVVGFKTPFLILCGLTLAAVALTPFAA
                     AAALRQAGD"
     gene            complement(1745..2353)
                     /locus_tag="VGB93_03660"
     CDS             complement(1745..2353)
                     /locus_tag="VGB93_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145248.1"
                     /translation="MKATAGVVLSLALAVTFASATRADAPHPVKLRGTILSLQGGVMS
                     ARGQDGAVIKIKIAPQTRLAAIVPASLAGIKPGSFVGITSMPGDGSERIALEVHIFPE
                     ALRGTGEGDRPWDKGPGSRMTNATVASVAGVHGEDLTLTYRGGQSKVKVTPRTRIVTF
                     LPGDAHDFKAGNHFIAFAIPDGQGAYVPLSLSIGRAGLTPPM"
     gene            2550..3305
                     /locus_tag="VGB93_03665"
     CDS             2550..3305
                     /locus_tag="VGB93_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY0145249.1"
                     /translation="MANYANPTQFLRLTRALVPVTAALTVVLLAVGLYLALVVAPADY
                     QQGETVRIMYLHVPSAWMAMFVYLVMSMAAIGTLVFRHPLADVAQKAAAPLGAGFTFL
                     CLTTGSLWGKPEWGTWWVWDARLTSVLVLFLIYLGIIALWQVIEDPGKAARLVAIFTL
                     VGAVNIPIIKFSVDWWHTLHQPSSVFRMGGPTIAASMLWPLLIMAVAFTTLFLTLHML
                     AIRNEIMRRRLRRLSMLAADGATPLHAMPEPAE"
     gene            3302..3469
                     /gene="ccmD"
                     /locus_tag="VGB93_03670"
     CDS             3302..3469
                     /gene="ccmD"
                     /locus_tag="VGB93_03670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY0145250.1"
                     /translation="MNFAAPHVGFVIAAYAIAFVVISVMVIGTLVDYRSLKKSLERVE
                     TRTRRTQDSNS"
     gene            3466..4077
                     /locus_tag="VGB93_03675"
     CDS             3466..4077
                     /locus_tag="VGB93_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY0145251.1"
                     /translation="MSVSSPSQDTPAEDAPARKPRRSRLAYLPLLIFIAVMGLLFVRL
                     FDGDPSRLPSALLNKSAPQFNLPALLTNAPGLSDKDLRQGHVTIVNIFASWCGPCHEE
                     NPQLVALSQDKRLAAEGVKLVGIAYKDQPENTRRYLGQEGDPYSVIGVDLSGRTGIDF
                     GVYGVPETYVIKGDGTIAFKFVGPIDDNTLENAIWPQIEAARK"
     gene            complement(4094..4855)
                     /locus_tag="VGB93_03680"
     CDS             complement(4094..4855)
                     /locus_tag="VGB93_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0145252.1"
                     /translation="MTGSDAAESPPEGQKFDPIAEAKRLLRSTRWASLATLVPESGSP
                     FATLVNVASAPDGSPILLISQLSAHRKHIAANPRVSLLYYLSKKGDPLAHPRLTLNGR
                     ASSVDEPAAGANLRARFLARHPKSALYADFPDFAFFRVELESVHMNGGFGRAAPGLTA
                     AQILTPIEGKSELIATEAGALANVNQDHADFAPLLAAAFGENSGHWRTLGFDPEGVDL
                     GNDDVILRLPFPQPIATPADLRQALVALAHAAREN"
     gene            5128..5823
                     /locus_tag="VGB93_03685"
     CDS             5128..5823
                     /locus_tag="VGB93_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145253.1"
                     /translation="MPTIALVDDDRNILTSVSIALEGEGYRVQTYTDGSTALDGLKTN
                     PPDLAIFDIKMPRMDGMELLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVRAILRRANPKEAAAQKESEAKILERGLLKMDPERHTCTWKNDPVTLTV
                     TEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVDDDFEM
                     IETLYGVGYRFKE"
     gene            5838..>7354
                     /locus_tag="VGB93_03690"
     CDS             5838..>7354
                     /locus_tag="VGB93_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEY0145254.1"
                     /translation="MSVEAHEQQFDQPLEAPRKRSRRRSFGWRVLRRVRTIQRAVAVR
                     FSSSLTRRIVVLNLGGLVALLLGFLYLNQFREGLIDARVQSLQTQGEIIAAAVAASAT
                     VATDSITVDPEKLLQMVPGERAGIADESRPSLEFSINPERVGPVLRRLVSPTRTRARI
                     YDRDGYLLLDSRALSARGDILRLDLPPQSDEAGNWLERVWAVMKRSFTYNELPLYEDI
                     GIANGRTYPEVSRALDGQAQSIVRMNNKGETIVSVAVPIELLRTVRGALLLSTQGGDI
                     DKIIASERWAIIRVFLVSAAIMLLLSLLLAGTIAGPMHRLAEAAERVRRGIKSRQEIP
                     DFTNRSDEIGHLSGALRDMTRALYNRMDAIESFAADVAHELKNPLTSLRSAVETLPIA
                     RNPESHARLLAIIQHDVRRLDRLISDISDASRLDAELARADMEPVDLSRIILTVVTMA
                     NHAEHESVQVTLDLANNEDAKKNSFIVLGHDSRLGQVFNNLIDNAKSFSQPAGSVR"
BASE COUNT         1288 a         2261 c         2300 g         1505 t
ORIGIN      
        1 tccgagcttc aacatggcat tgggcatcgc cgtgtgattg acgacggcgg gtatgccggt
       61 gaaattctgc atcaaaacgc gcgactgccg gaaagcgatt tccttctcga ccttgccctt
      121 gttgacgagc cattcggcga cggattggat cgattctttg gtaaccgagc ggccatcctc
      181 gaaacgcagc agattttcga gcaggacttt catcgaaaaa ggcagagagg aaatgccggt
      241 cagaccattc ttctccgccg tcttgagcga ataataatga taggtcttgg caccgacgtt
      301 gaggattttg cggcacttaa aagaatcgag cgaagccata ggctacgggg cccctgatgt
      361 tgagaaacgc aatgatgggt ggtttataga taatttctaa tctggtcgct acaccaccat
      421 tcgccgcgcc atgcaggccg cgcgccataa ggatcgagcc gcgccgtcat gcagatcgaa
      481 gcctcggctc tcagcgtcga acgcggcggg cgcattattt tcgcgggcct cgacttccgg
      541 ctggtggccg gcgaagcgct tctggtcaaa gggccgaatg gcgccgggaa atcgacgctt
      601 ctgcgcgcct tcgcgggctt tctgcctctg gcggcgggtg ccatctccat cacgcctgaa
      661 acggaggcgc gccgggcgga gctttgccat tatattgggc atgccgacgc actgaagccg
      721 gcactgacgc tcgccgaaaa tctcgccttc tataccgcct tgctcggcgg cggcggcatc
      781 gagcgcgcgc ttgcgcgtct cggactgaca gggatcgccg atcttcccgc aggctatctg
      841 tcggccgggc agaaacgccg cgcggctctg gcgcggcttg tcgcgatcgc gcggcccatc
      901 tggctgctcg acgagccgct gacggcgctc gatgccagat ctcaaaccgt agtcggcgag
      961 ctgatgcagg agcatctcgc cggcggcggc tgcatcctcg ccgcgaccca tacgcctttg
     1021 ccggttgccg cgcgcgatct gatgctcggc cggccggtat gacggaaagc cagcaagtcc
     1081 gtccttcggc gctcggcgcc ctcctgacgc gtgaattgcg cgtcgggcgt cggatcggcg
     1141 gcgcggtcag catgggcgtc gtgttctttc tgtgcctcgt gacggtgacg cccttcgcgg
     1201 tggggccgga tcaggcactg ctggcgcgga tcggtccggc gatcctctgg atcgcggcat
     1261 tgctggcgac gctgctcggt ctcgatcggc tgtttcaggc cgatgctgag gacggttcgc
     1321 ttgatctttt tctgaccagc gagacgccac tcgaactcat cgttcttgtc aaatgcctgg
     1381 cgcattggct gctgaccggc ctgccgcttg ttgcggctag cccgatcttc ggtctgatgc
     1441 tcgggctcgg gccggatgga ttggtgcgcg tcgcgctctc gctgctcgca gggacgccgg
     1501 cgctgtccct tctcggcgcg atcggcgcgg cgctgacggt gagcctgcgc cgcggcggtc
     1561 tgctgctggc cgtgctcatt ctgccgctga gcgtaccggt cctgattttc ggtgtggcgg
     1621 cggccggcga tcctgtggtc ggcttcaaga caccgttcct gatcctctgc ggcctgacgc
     1681 tcgccgccgt agcgctcacg cccttcgccg ctgccgcggc gctgcgccag gccggggact
     1741 aacgctacat cggcggcgtg agaccggcgc ggccgatgct gagcgagagc ggcacataag
     1801 caccttgccc gtccgggatg gcgaaggcga tgaaatggtt gccggctttg aagtcatggg
     1861 catcaccggg caggaatgtc acgatccggg ttcgcggcgt cactttgact ttggattggc
     1921 cgccgcggta ggtgagcgtc aaatcctcgc cgtgaacgcc cgcaaccgac gcgacggtgg
     1981 cattcgtcat ccggctgccg gggcctttgt cccaggggcg gtcgccttcc ccggtcccgc
     2041 gcagagcttc gggaaaaata tgcacttcca gcgcgatccg ttcgctgccg tcgcccggca
     2101 tgctggtgat gccgacaaag ctgcccggct tgatcccggc gagcgaggcc ggaacgatag
     2161 cggcaagacg cgtttggggc gcgattttga tctttatgac ggcgccatcc tgcccacggg
     2221 ccgacatgac accgccttgc agcgagagga tcgtgccgcg cagcttcacg ggatgcgggg
     2281 cgtcggcgcg cgtcgcgctc gcgaatgtca cggcgagcgc gaggctcaag accacaccgg
     2341 ccgtcgcctt cacggcgtgg cctctgttaa acattttccg catttgatct ccaaaaaggg
     2401 atgtcggcga tgtcgccggt gataccccga acttcgtctg cccggccgga ttatcgacga
     2461 attcacgtcc caaggctgat cagcttttgg tgaaccaaaa aaccgcgaaa catttgcgtt
     2521 ccggtatggg gcggcctaga ggaaaagcga tggcgaatta cgctaacccg acgcaatttc
     2581 tgcggctgac gcgcgcgctc gtgccggtca cggcggcact gacggtcgtg ttgctggcgg
     2641 tcgggcttta tctcgcgctt gtcgtggccc cggcggatta tcagcagggc gagacggtgc
     2701 ggatcatgta tctgcacgtg ccgtcggcgt ggatggcgat gttcgtctat ctggtgatgt
     2761 ccatggcggc gatcggcacg ctggttttcc gccatccgct cgccgatgtg gcacagaagg
     2821 cggcggcgcc gcttggcgcc ggattcacct ttctgtgtct gactacgggt tcgctctggg
     2881 gcaagccgga atggggcacc tggtgggtct gggatgcgcg gctgacgtcg gtgctcgtcc
     2941 ttttcctcat ctatcttggc atcatcgcgc tctggcaggt gatcgaggat ccgggcaagg
     3001 cggcgcggct cgtcgccatt ttcacgctcg tcggcgcggt caatattccg atcatcaaat
     3061 tctcggtcga ttggtggcac acgctgcatc aaccgtcgtc ggtcttccgc atgggcggcc
     3121 cgaccatcgc cgcctcgatg ctctggccgc tcctgatcat ggcggttgcc ttcacgacgc
     3181 tgttcctgac actccatatg ctggcgatcc gcaatgaaat catgcgccgc cgcctgcggc
     3241 gtctttcgat gctcgcggcc gatggcgcca cgccgcttca cgcaatgccg gagccggccg
     3301 aatgaatttt gccgccccgc atgtcggttt cgtgatcgcc gcttatgcca tcgctttcgt
     3361 cgtcatcagc gtcatggtga tcggcacgct ggtcgactat cgttcgctca agaaaagcct
     3421 tgagcgtgtc gagacgcgaa cccgccgcac gcaggattca aattcttgag cgtcagttcg
     3481 ccgtcccaag acacgcctgc cgaagatgcg cccgcgcgga aaccgcgccg ctcgcggctc
     3541 gcctatctgc cgctgctcat cttcattgct gtgatgggct tgcttttcgt gcggctcttt
     3601 gatggcgatc cgtcgcggct gccttcggcg cttctgaaca aatccgcacc gcagttcaat
     3661 ttacccgcgc tgctcacgaa cgcccccggt ctctcggaca aggatttgcg gcaaggccat
     3721 gtgacaatcg tgaacatctt cgcctcgtgg tgcggcccgt gccatgaaga aaatccgcag
     3781 ctcgtcgcgc tctcgcagga caagcgcctc gcggcagagg gcgtcaaact ggtcggcatc
     3841 gcctacaagg atcagcccga gaacacgcgc cgctatctcg gccaggaggg cgatccctat
     3901 tcggtgatcg gcgtcgattt aagtgggcgc acgggcatcg acttcggcgt ttacggtgtg
     3961 cccgagacct atgtcatcaa gggcgacggg acgatcgcct tcaaattcgt cggaccgatc
     4021 gacgacaata cgctggaaaa cgccatctgg ccgcagatcg aagctgcgcg gaagtaatca
     4081 tctatcccgg tcgctaattc tcgcgcgcgg cgtgcgccag ggccacgagt gcctggcgca
     4141 aatctgctgg cgtcgcgatc ggctgcggaa atggcaggcg taaaatcaca tcgtcattgc
     4201 cgagatcgac cccttccggg tcgaagccga gggtgcgcca atggccgctg ttttccccga
     4261 acgcggccgc gagtagcggg gcgaaatcgg catgatcctg gttcacattc gcaagtgcgc
     4321 cggcctcagt ggcgatgagc tcactcttgc cctcgatcgg tgtgaggatt tgcgcggcgg
     4381 tcaatccggg cgccgcgcgg ccgaaaccgc cgttcatatg gacgctttcg agttcaacac
     4441 ggaaaaatgc gaaatcggga aaatcggcat agagcgccga ttttggatgc cgggcgagga
     4501 agcgcgcccg caaattcgcc cccgccgccg gttcatcgac gctggacgcg cgcccgttga
     4561 gggtcagccg cggatgagcg agcggatcgc ccttcttcga cagatagtag agcagcgaga
     4621 cgcgggggtt ggcggcgata tgttttcgat gcgcggaaag ctgcgatatc aagaggatag
     4681 ggctgccatc cggggcactg gcgacattga cgagggtggc gaagggagaa cccgactccg
     4741 gcaccagcgt cgccagcgag gcccagcgcg tcgaccgcag aagccgcttg gcctcggcga
     4801 tgggatcgaa tttctggcct tcgggcgggc tttcggccgc gtcggaaccg gtcatcggca
     4861 agtcctcgtt tcggggcggc ggaagctaaa gctttgcttt caaaggcttc agggctggat
     4921 tcccctatag ttctaatggt atgcattgca aagatgcggg gaatggcctc atttcggccc
     4981 tcaagctcac ggagcttccc gctgcgtgcc gcgtggggcg gaagaggcgt ccctcacgtc
     5041 gtgttcatgc gctggcggtt gaactacgtc gctctgccaa ccggcagctt cttacgagat
     5101 tcggcccttt aatcctgagg agtgttgatg ccgacgatcg ctttggtcga tgacgaccgc
     5161 aacattctca catcggtttc catagccctc gaaggcgagg gatatcgggt gcaaacctac
     5221 accgatggct cgacggcgct cgacggcttg aagaccaatc cgccggatct tgccattttc
     5281 gatattaaaa tgccgcgcat ggacggcatg gagcttttgc ggcggctgcg gcaaaagtcc
     5341 gatctgccgg tgatctttct cacctcgaag gatgaggaaa tcgacgaatt gttcggcctc
     5401 aagatgggcg ccgacgattt catccgcaaa cccttttccc aacgcctgct cgtcgagcgc
     5461 gtccgcgcca tcctgcgccg cgccaatccc aaggaagcgg cggcgcagaa ggaatccgag
     5521 gccaagattc tcgagcgcgg cttgctcaag atggaccccg agcgccacac ctgcacctgg
     5581 aaaaatgatc cggtgactct caccgtgacc gaattcctta tcctccaggc gctcgcgacg
     5641 cgtcccggcg tagtgaagag ccgcaacgcg ctgatggatg cggcttacga cgatcaggtc
     5701 tatgtcgacg accgcacgat cgacagccac atcaagcgtc tgcgtaaaaa attcaaagcc
     5761 gtcgatgacg acttcgagat gatcgaaacg ctttacggcg tcggttatcg tttcaaggaa
     5821 tgatcgcggc gaactgaatg agcgtcgaag cgcacgagca gcagttcgat cagcccctgg
     5881 aagcgccgcg caagcgtagc cgtcggcgca gtttcggctg gcgcgtttta cggcgcgttc
     5941 gcacgatcca gcgggcggtc gcagtccgtt tttcgtcatc gctgacgcgc cgcatcgtgg
     6001 ttctcaacct cggcggcctc gtcgcgttgc tgctcggttt tctttatctc aatcagtttc
     6061 gcgaaggtct catcgacgcg cgagtgcaga gcctgcagac gcaaggcgag atcatcgccg
     6121 cggcggttgc ggcctcggcg actgttgcga cggattcgat caccgttgat ccggaaaagc
     6181 ttctgcagat ggtgcctggc gaacgtgccg gcattgccga cgaaagccgg ccgtcgctcg
     6241 aattttccat caatcccgag cgcgtcgggc ccgtgctgcg ccgccttgtt tcgccaacgc
     6301 gcacgcgggc gcgcatttac gaccgcgatg gttatctgct gctcgattcc cgcgcgctga
     6361 gcgcccgcgg cgacatcctt cggctcgatt tgccgccgca gagcgacgag gcaggaaact
     6421 ggctcgaacg cgtctgggcc gtgatgaaac gctcgttcac ctataacgaa ttgccgctct
     6481 acgaagatat tggcattgcc aacggtcgga cctatcccga agtctctcgc gcgctcgacg
     6541 gccaggcgca gtcgattgtg cggatgaata acaagggtga gacgatcgtc tccgtcgcgg
     6601 tgccgattga gcttttgcgc accgtccgcg gcgcattgct gctttcgacg caaggcggcg
     6661 acattgacaa gatcatcgct tcggaacgct gggcgatcat ccgcgtcttc ctggtttcgg
     6721 ccgcgatcat gctgctcttg tctctgctgc tcgcgggcac gatcgcgggg ccgatgcacc
     6781 ggctggcgga agcggccgag cgggtacgcc gcggcatcaa atcacgccag gaaattccag
     6841 attttaccaa tcgcagcgac gagatcggcc atttgtccgg cgcgctgcgc gacatgacgc
     6901 gggcgctcta taatcgcatg gacgcgattg aaagtttcgc cgccgatgtg gcccacgaac
     6961 tcaagaaccc gttgacctcc ttgcggagcg ccgtcgagac tttgccgatc gcccgcaatc
     7021 cggaatcgca tgcgcgactt ttagccatta ttcagcatga cgtgcgccgg cttgatcggc
     7081 tgattagcga tatatcggat gcgtcgcggc tcgatgccga actggcgcgc gccgacatgg
     7141 agccggtcga tctctcgcgt atcatcctca ccgtggtgac gatggccaat catgcggagc
     7201 atgaaagcgt gcaggtgacg ctcgatctcg ccaacaatga agatgcgaaa aagaacagtt
     7261 tcatcgtcct cggccatgat tcgcgtctgg gccaagtttt caacaacctc atcgataacg
     7321 cgaaatcctt ttcacagccc gccggcagcg tgcg
//
LOCUS       DASWOG010000180         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_315963,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000180 DASWOG010000000
VERSION     DASWOG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315963"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGB93_03695"
     CDS             complement(<1..1005)
                     /locus_tag="VGB93_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0145255.1"
                     /translation="MTSQQRSVVSRRSVLQLCSGLIGASIPFRSIRVLADVPAGGSEM
                     HGLSIFGDLALPKDFQSFAYVNPQAPKGGQLALQPSGGGANVSPTTFNSFNDYILKGD
                     GAAGMGAIFDSLMAGNSDEPDALYGLVAEKVWISTDKLTYRFFLRKQVRFHDGTPLTA
                     HDVVFSLNILKTQGHPAISQSLRDLESAVAEADDIVRVSLRKGHGREAAMIIAGQPIF
                     SAAYYGKNAFNETTLQPPLGSGPYKVGAFDAGHFIVFERVADYWGKDLPVNVGQNNFD
                     RIRYEYFADRKVAFEAFKAGVFTFREEFTSAVWATQYGFAAANDGRVKRETLPDQLPL
                     G"
     gene            complement(1155..1700)
                     /locus_tag="VGB93_03700"
     CDS             complement(1155..1700)
                     /locus_tag="VGB93_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0145256.1"
                     /translation="MNSFEFNKIAGGVLGTLLFAQVVYLASNALFSHPAPPKPAESAA
                     TDAGKPAAAPAPAPAELLAAYIGKADVKKGEDDSKPCQICHNFAKGGGIILGPPLYGV
                     VGRARASFAGFQYSDAMKAKGGTWTPQDIFIFIKDPQAFVPGTKMTFSGEPSPEKRAD
                     IVAYLDTLSDHPVPLPAPAAH"
     gene            1930..2790
                     /locus_tag="VGB93_03705"
     CDS             1930..2790
                     /locus_tag="VGB93_03705"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY0145257.1"
                     /translation="MTNRKIAYQGEPGANSDIACRAVYPDFEPLPRATFEDALTAVSD
                     GSADYAMIPIENSLAGRVADIHALLPTAGLYIVGEYFLPVHFQLLGIKGAKIDGLKSV
                     YSHVHALGQCRKIIRKLNLVAHISGDTAGSAKEIAEWRDPTKAALAPALAAKIYGLDI
                     LAADVEDEKHNTTRFVILARAPHWPERGSVATVTTFIFRVRNVPAALYKALGGFATNG
                     VNMTKLESYMVDGEFTAPQFLADVDGHPDDPALARAFEELAFFCKELKILGVYAPHPF
                     RTESVAAIVK"
BASE COUNT          638 a          909 c          889 g          591 t
ORIGIN      
        1 tcccagcggc aattgatccg gcaatgtctc gcgtttcaca cggccatcgt tggccgcggc
       61 aaagccgtat tgcgtcgccc agacggcaga ggtgaattcc tcgcggaacg tgaaaacgcc
      121 ggccttgaag gcttcgaaag caaccttgcg atcggcaaaa tattcgtagc ggatgcgatc
      181 gaaattgttc tgcccgacat tgaccggcag gtccttgccc caataatcgg caacgcgctc
      241 gaagacgatg aaatgacccg cgtcgaaagc gccgaccttg tatggaccgg agccaagcgg
      301 cggctgaagc gtcgtctcgt tgaatgcgtt cttgccgtaa taggctgcgg agaaaatcgg
      361 ctggccagcg atgatcatcg ccgcttcgcg accgtggccc ttgcgcaatg acacgcgcac
      421 gatgtcatcg gcttcggcga ccgcactttc gagatcacgc aaggattgcg agattgccgg
      481 atggccctgc gttttgagga tgttcagcga gaagacgacg tcatgcgccg taagcggcgt
      541 tccatcgtga aagcgcacct gcttgcgcag gaagaatcga taagtcagct tgtcggtaga
      601 gatccagacc ttttcggcga caaggccgta aagcgcgtcc ggttcgtcgc tattgcccgc
      661 catcaacgag tcgaagatcg cgcccatgcc ggccgcgccg tcacccttta aaatataatc
      721 gttgaaggaa ttgaacgtcg ttgggctgac gttggcgccg ccgccgcttg gctgcaaggc
      781 gagctggccg cccttcgggg cctgtggatt gacgtaagcg aaggactgaa aatctttcgg
      841 caatgccaga tcgccgaaga tcgacaatcc atgcatttcg ctgccaccgg ccggcacatc
      901 ggccagaacc cggatggacc tgaatggaat gctggcgccg atgaggccgg agcaaagttg
      961 gagaacggag cggcgggaaa cgaccgaacg ttgctgcgat gtcaaaaaag cctctctgac
     1021 gctttgcgag atgcggtcag actgtccgag gattatgtcg ctttttaacc cggcagcgca
     1081 acacgagacc gtccgcgata acaaaacggc cgcgcaagcg cggccggtct tcatggagct
     1141 ttagccagga ccttttaatg cgcggcgggt gctggcagcg gcaccggatg gtccgacaac
     1201 gtgtcgagat aagcgacgat gtcggcgcgc ttctcaggcg agggctcgcc ggagaaggtc
     1261 atcttcgtgc ccggcacaaa cgcctgcgga tccttgatga agatgaaaat gtcttgcggc
     1321 gtccaggtgc cgcccttggc cttcatcgca tccgaatact ggaagcccgc gaaagaggcg
     1381 cgcgcccgcc cgacaacgcc atagagcggc ggcccgagga tgatcccgcc gcccttcgcg
     1441 aaattgtggc agatctgaca gggcttggaa tcgtcctcac cctttttgac gtcggccttg
     1501 ccgatgtagg cggcgagaag ttcggcgggc gctggcgctg gcgccgccgc gggcttccca
     1561 gcgtcggtcg cagccgattc ggctggcttg ggcggagccg gatgcgagaa taatgcgttc
     1621 gacgccagat aaaccacttg agcaaaaaga agcgtgccga gaacgccccc cgcgatcttg
     1681 ttgaactcaa aagaattcat ggccttacga actcccccgc agagccggca tttgacggcg
     1741 atcatattag tggtcgttga ttagtcgctt tgccgcagcc ttggcaaccc gtataaggcg
     1801 gccaataata attttcctgc gggtaaagac ttcgccagta agttcgggaa cttatccaag
     1861 cggcggtcct tcctggagcg ccgaggcagc taaagcgtct tccgcggacg cgcgcttctg
     1921 agacaagaga tgacgaacag aaaaatcgct taccagggcg agcctggcgc caattccgac
     1981 atcgcctgcc gcgccgtcta tccagatttc gagcccctgc cccgcgccac attcgaggac
     2041 gcgcttactg ccgtctccga tggctccgcc gattacgcga tgatcccgat cgagaactcg
     2101 ctagcggggc gcgtcgccga tatccatgcg ctcttgccca ccgcgggact ttatatcgtc
     2161 ggcgaatatt tcctgccggt gcatttccag ttgctgggga tcaagggcgc gaaaatcgat
     2221 ggcctcaaat ccgtttacag ccacgtccac gcgctcggcc aatgccggaa aattatccgc
     2281 aagctgaacc tcgtcgccca tatcagcggc gacaccgctg gttcggccaa ggagatcgcc
     2341 gaatggcgcg atccgaccaa ggccgcgctc gcgccggcct tggccgcgaa gatttacggc
     2401 ctcgacatcc tcgcggctga cgtcgaagac gagaagcata atacgacgcg gttcgtcatt
     2461 ctggcgcgcg cgccgcattg gccggaacgc ggcagtgtcg cgactgtcac gacgttcatc
     2521 ttccgcgtcc gtaacgtccc ggcggcgctt tataaagcgc tcggcggttt cgccaccaat
     2581 ggcgtcaaca tgaccaagct cgaaagctat atggtcgacg gcgaattcac cgcgcctcaa
     2641 tttcttgccg acgtcgacgg ccaccccgat gatcccgccc ttgcccgggc cttcgaggag
     2701 ctggctttct tctgcaagga actcaaaatt ttgggcgtct acgcaccgca ccccttccgc
     2761 accgagagcg tcgccgcaat cgtgaaatga tgctttttgg gcacgcttcg aagcgtaaag
     2821 cgctcatgtg ctccaaagcg gggcgctggc cgtattactg ccatcaaggc caacttaacc
     2881 atcttggccg atggcccaaa tgcaacgctt ttcaagaagg tctcataaag gttgacggaa
     2941 ccttcgttta accatcgccc ggtaggacta tcggatcgag tgccatgaaa ggagttttgt
     3001 catgagcctt cacccgcgca cgggagc
//
LOCUS       DASWOG010000181         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_297724,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000181 DASWOG010000000
VERSION     DASWOG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297724"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VGB93_03710"
     CDS             complement(<1..588)
                     /locus_tag="VGB93_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HEY0145258.1"
                     /translation="MNIQSQTVKKAAGKTDLGHARTFDLATNSVRELNRALHAIHPGS
                     NANLFRVLNPRGVHAIAAGVDADVTIEIDGDVGYYCGGMNQLATILIEGSAGIGLGDN
                     MMSGLIHVRGNASDSVGATAHGGSIIIDGDASGRCGISMKGVDIIVKGSIGPMSAFMA
                     QAGTLAVFGDVGEAFGDSLYEAKLYARGNVASLGAD"
     gene            complement(585..1475)
                     /locus_tag="VGB93_03715"
     CDS             complement(585..1475)
                     /locus_tag="VGB93_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HEY0145259.1"
                     /translation="MCGIVGLFFKDPALEPDLGKHLSVMLETMTARGPDSAGFAVYGE
                     GEKSRTKITLRAPADFESYGALEKALEPGASVTRRGTHIVVSVPTTDVEGVLQKIKHE
                     APTLTVSSVGTHMELYKEVGLPADVAKKFHLAEMSGTHSIGHTRMATESAVKTAGAHP
                     FSTGKDQCLVHNGSLSNHNQMRRKLIREGIPFATENDTEVAAGYVSWRLAQGDSLGEA
                     LESSLKDLDGFYTFVVGTDTGFGVLRDPIGCKHAVMAETDQYVAFGTEFRALSSLPGI
                     ENAKIFEPNPATVYFWNRAA"
BASE COUNT          335 a          477 c          475 g          330 t
ORIGIN      
        1 atcggcgcca aggctcgcga cgtttccgcg cgcataaagc ttcgcctcat agagcgagtc
       61 gccgaaggct tcgccaacat cgccgaagac ggcaagcgtg cccgcttgcg ccatgaaggc
      121 cgacatcggg ccgatggagc ccttcacgat aatatcgacg cccttcatgg aaatgccgca
      181 gcgccccgag gcatcgccat cgataatgat cgaaccgcca tgcgccgtcg cgccgacgga
      241 atcgctggca ttgccgcgaa cgtggatcag ccccgacatc atattatcgc cgaggccgat
      301 gccggcgctg ccttcgatga ggatcgtcgc cagttgattc ataccaccgc aataatagcc
      361 gacgtcacca tcgatctcga tcgtcacgtc cgcatcgacg cccgccgcga tggcatgaac
      421 gccacgcgga ttgagcacgc gaaataaatt cgcattgctg cctgggtgga tcgcgtgcag
      481 tgcacggttc aactcgcgca cagaattcgt cgcgagatca aacgtgcgag catggccgag
      541 atcggttttg cccgcagctt tcttcacggt ttgcgattga atgttcatgc cgcgcggttc
      601 caaaaataga cggtggcggg attcggctcg aaaatcttcg cgttctcaat gccgggcagt
      661 gaactcaggg cgcgaaactc ggtgccgaag gcgacgtatt gatcggtctc ggccataacg
      721 gcgtgtttgc agccgatcgg atcgcgcaga acgccgaagc ccgtgtcggt gccgacgacg
      781 aaagtataga aaccgtcgag gtctttcagc gaggattcga gcgcttcgcc aagactatca
      841 ccctgcgcaa ggcgccagga cacatagccg gcggcgactt ccgtgtcgtt ttcggtcgcg
      901 aagggaatgc cttcgcggat cagcttgcgg cgcatctgat tatggttcga cagcgagccg
      961 ttatggacga ggcattgatc cttgccggtg gagaatggat gcgcgcccgc ggtcttcacc
     1021 gccgattcgg tcgccatgcg cgtatggccg atgctatgcg tgccgctcat ttcagcgagg
     1081 tggaatttct tggcaacgtc ggcaggcagg ccgacctctt tataaagctc catatgcgtg
     1141 ccgacggagc tgaccgtgag cgtcggagcc tcgtgcttga tcttctgcag aacgccttcg
     1201 acatcggttg tcggcacgga gacaacaatg tgggtgccgc ggcgcgtgac gctggcacct
     1261 ggctcaagcg ccttttccag ggctccgtag ctttcaaaat ccgccggcgc gcgcagagtg
     1321 atcttggtgc ggcttttctc gccctcgcca tagacggcga aaccggcgct gtcggggccg
     1381 cgcgccgtca tcgtctccaa catgaccgag agatgcttgc cgagatccgg ctccagagct
     1441 ggatctttaa agaagagtcc aacgatgccg cacatcccac tgcctcgcgt gtttcctgaa
     1501 cctaaagcaa ttttcctata aagaaaatag cagtcgcgtg aagaaactgg aagggggatt
     1561 ttgcgctttt cgtcaaaaaa attacgtttg cgctacggca aagccggtct tgcataa
//
LOCUS       DASWOG010000182         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_77347,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000182 DASWOG010000000
VERSION     DASWOG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77347"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGB93_03720"
     CDS             complement(<1..208)
                     /locus_tag="VGB93_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEY0145260.1"
                     /translation="MKRLTEFVWPAIGVVALVVSIWLLDQQFRGQAIGPKVLADLKTI
                     PLPQYGLAILSTLVAYAALAWYDRI"
     gene            complement(398..808)
                     /locus_tag="VGB93_03725"
     CDS             complement(398..808)
                     /locus_tag="VGB93_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY0145261.1"
                     /translation="MKSRDSLIRLKRFQVEERRRRMAQIEAMIAEFARMAADLDREIA
                     LEEQRAGISDMAHFAYPTYARAARARRDNLQRSSGELREQLEAAKALFDEAAAELSKA
                     TSLEGREKAADRGHEALDQGAPPIVNFDLRVARA"
     gene            1151..1852
                     /locus_tag="VGB93_03730"
     CDS             1151..1852
                     /locus_tag="VGB93_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145262.1"
                     /translation="MRVLLIEDDSATAQSIELMLKSENFNVYTTDLGEEGIDLGKLYD
                     YDIILLDINLPDMSGYEVLRTLRVAKVKTPILILSGLAGIEDKVKGLGFGADDYLTKP
                     FHKDELVARIHAIVRRSKGHAQSVIATGDLIVNLDQKTVEVSGNRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLANASRGRNYIETVWGRG
                     YVLREPSEDEELVSA"
     gene            complement(1905..2759)
                     /locus_tag="VGB93_03735"
     CDS             complement(1905..2759)
                     /locus_tag="VGB93_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY0145263.1"
                     /translation="MNSDFDVLRQLLNRRSGLSLDAEKAYLAESRLQPLVQQLGLTGL
                     GGLVKMLLSGAHEDLEREVVEAMTTNETFFFRDRVPFDNFRKVILPHLLEARRDSRQI
                     RIWCAACSSGQEPYSLAMLLDEEAHRLAGWSIEILATDLSRAVVNAARQGVYSQFEVQ
                     RGLPIAQLLRYFRQDGERWRINEHLRSRLRFTEFNLLSSYAALGRFDVIFCRNVLIYF
                     DVPNKKDVLDRMAGALAPDGFFIMGAAETVVGLTETMVPHQLHRSINIHRAAALPAAR
                     PSLTLVAS"
     gene            complement(2756..>3021)
                     /locus_tag="VGB93_03740"
     CDS             complement(2756..>3021)
                     /locus_tag="VGB93_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0145264.1"
                     /translation="RSAAEVFGPAVIGIVLTGMGSDGLAGTRAIVAAGARVIAQDAAS
                     SVVWGMPRLVASEGLAQAVLPLKEIGVSVCRLVRHAQFREQKS"
BASE COUNT          627 a          878 c          911 g          605 t
ORIGIN      
        1 cgattcggtc gtaccaggcc aatgctgcgt aagcgacgag ggtcgaaaga atcgccagcc
       61 catattgcgg cagcggaata gttttaagat cggccagaac ctttggcccg atcgcttggc
      121 cacgaaactg ctggtcgaga agccaaatcg acacgacaag tgcgacaaca ccgatggcag
      181 gccagacaaa ttcggtgagc cttttcatgt ctctcctgaa atccgcgcgc cgcggcgggg
      241 cgatcggccg cccctcgctt ccagcctgtg gagaccgtaa agcgtcatac aaagcaagcg
      301 ctcattttcc gccagggagg cgacaatttg acggcgcgcc cgaggcttgg ccgtcatcgc
      361 ggctttaagg cgccggctcg attagccaat gatgatttta ggcgcgcgcg acgcgcaggt
      421 caaagttgac gatgggaggc gcgccttgat ccagcgcctc gtggccccgg tcggcggctt
      481 tctcgcgtcc ttcgagactc gtagccttgc tgagctcggc cgcggcttcg tcaaaaagcg
      541 ctttggcggc ctcaagctgt tcgcgcaatt cgccgctcga acgctggagg ttgtcgcgtc
      601 gcgcccgggc ggcccgcgcg taggtcgggt aggcaaaatg cgccatatca gagatgccgg
      661 cgcgctgttc ttcgagcgcg atttcacggt cgaggtcggc agccatgcgc gcaaattcgg
      721 cgatcatcgc ctcgatttgg gccatgcggc ggcggcgctc ctcgacttgg aagcgcttga
      781 gacggatgag cgaatcccgc gacttcatat tttgttactc cgaaacgcca gccagatctc
      841 gatctgttta gtcaattttt ctgcgagacg agggcgaata tgacctatta aagtttctgc
      901 tttcttacgc ttgtcatcct tacgcttgtc ttcgcgccgc tggcgattcg gtgacgatcg
      961 agtatatttt tatcgcgtgc ctgattcggg acggaattct agggcgttcg gaatgatttt
     1021 gttaaccaat gcccgttaac tttaagaaag tggttcggca gatttattct cgctgactcc
     1081 cgcgctgggg cggggggtct tgggaactgg tttcgcgcgc cgaccgacga gggaaggctg
     1141 agggctccac atgcgcgtgc tcctgataga agacgacagc gccacagcgc aaagcatcga
     1201 actgatgctc aagtcggaaa atttcaacgt ctacacgacg gaccttggcg aagagggaat
     1261 cgatctcggc aagctctacg actacgacat catccttctc gacattaatc ttcccgacat
     1321 gtccggttac gaagttctgc ggacgctgcg ggtcgctaag gtcaagacgc ccatcctcat
     1381 tctctccggc ctggccggca tcgaggataa agtgaagggc ctgggcttcg gcgccgacga
     1441 ttatctaacc aagcctttcc ataaggatga actggtcgcc cgcattcatg cgatcgtccg
     1501 ccgctccaag ggccacgccc aatcggttat cgcgaccggc gacctgatcg tgaacctcga
     1561 tcagaagacc gtcgaggttt ccggcaaccg ggtgcacctt actggcaaag aatatcagat
     1621 gctggagctt ctctccctgc gcaagggcac gaccctgacc aaggagatgt tcctcaatca
     1681 tctttatggc ggcatggatg agccggaact gaaaatcatc gacgtcttta tctgcaagct
     1741 gcgcaagaaa ctcgccaacg cgagccgcgg ccgcaattat atcgagacgg tctggggccg
     1801 cggctatgtg ctgcgtgaac cttcggaaga cgaggaattg gtttcggcgt aagacagacg
     1861 ccacggaatg aaaaaagccc cgcttcgagc ggggcttttt tgtttcagct tgcgacgagc
     1921 gtcaatgacg gccgcgccgc gggcagggcg gccgcgcgat gaatattaat tgagcgatgc
     1981 aattgatgcg gcaccatcgt ttccgtcaat ccgacgaccg tttccgccgc gcccatgatg
     2041 aagaagccat ccggggcgag cgctccggcc atgcggtcca aaacgtcttt cttgtttggc
     2101 acgtcgaagt agatcagcac gttgcggcaa aagatgacgt caaagcggcc cagcgcagcg
     2161 tagctcgaaa gcaaattgaa ttcggtgaag cggaggcgcg agcgtagatg ctcgttgatg
     2221 cgccagcgtt cgccgtcctg ccggaaatag cgcaggagct gggcgatcgg gagaccgcgt
     2281 tgcacctcga attgcgagta cacgccttgg cgcgcagcat tgacaacggc gcgcgagagg
     2341 tcggttgcca gaatttcgat gctccagcca gccagccggt gcgcttcctc gtcgagcagc
     2401 atggcgagcg aatagggttc ctggccggac gagcaggccg cgcaccagat gcggatctga
     2461 cggctgtcgc gacgcgcctc taacaaatgc ggaaggatga ctttccggaa attgtcgaac
     2521 ggcacgcgat cccgaaagaa aaaggtctcg ttggtcgtca tcgcctcgac gacctcgcgc
     2581 tcgaggtctt catgcgcgcc ggatagcagc attttgacga ggccgcctag tcccgtgagt
     2641 ccgagctgtt gcacgagcgg ctgcaaccgg ctttctgcga gataggcctt ctccgcatcg
     2701 agcgagagcc ccgaacgccg gttgagcaat tggcgaagca cgtcaaaatc ggaattcatg
     2761 atttttgctc tcgaaactgt gcgtggcgaa caagacggca gaccgagacg ccgatttcct
     2821 ttagtggcag gaccgcctgc gccagtcctt cgctggcgac aagacgtggc ataccccaca
     2881 cgacggacga ggccgcatcc tgtgcgatga ccctggcgcc ggcagcgacg atggcgcgcg
     2941 tcccggcgag gccgtcgctg cccatgcccg tgagcacaat gccgatgacc gccggaccga
     3001 agacctctgc agccgagcgg a
//
LOCUS       DASWOG010000183         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_297760,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000183 DASWOG010000000
VERSION     DASWOG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297760"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGB93_03745"
     CDS             <1..685
                     /locus_tag="VGB93_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0145265.1"
                     /translation="STRRLVRIAREQHASIHVLHISTAEEIDFLAQHKDVASVEVTPQ
                     HLTLSADDYARLGTRLQMNPPIRAPRHRERIWYGLHQGIADILGSDHAPHTLEEKAKP
                     YPQSPSGMTGVQTLVPIMLDHVNAGRLTLERFVDMTSAGPARLFGIAGKGRMAVGYDA
                     DFTLVDLKRRETITDAWSASRSGWTPYDGVTVTGWPVGTIVRGQRIMWEGEIMTSGQG
                     EAVRFIGGL"
     gene            744..2168
                     /locus_tag="VGB93_03750"
     CDS             744..2168
                     /locus_tag="VGB93_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145266.1"
                     /translation="MRFNRALIPALLVWAFSFAAQAQPAPQSGSDKAPTILACFNAGQ
                     TAIRDLRACAGAWLTPKALLLCALQNTDAGKADAIACPFLPDTPGGRAILDVLLVQQY
                     LTPESNLSLDTRNFPGLPGAAVVDVCNRTAATRGDFLACIQTSTSQAQTQALLQCIKT
                     SNSEGGRALCLAKSATGNPDLPGAVKCFGVQGGAPDKFVSCLGAPDRVAAARAAIGCA
                     ASGISPSALADCILPSASPAEKALASCLIQSQDDDRNVAGCLGHFSPAMGRSEQASAC
                     FSDPGNSAAKCAQELLGDTTHVAETVAACAATRRNALVGCFLDKRPELAVTRNLYTCF
                     ADGGEGSSLVASCTGELIPDEKIRRTLACITAANNDPAALAVCASGSVLPRDAAHPVE
                     CAATSQDSPVSFALCAAAPKTDDDWRISAQCAIASAGDSKAYAGCMGMPLAINELGKC
                     FKGKFGKDCFGPTNSIAKILNVAQ"
BASE COUNT          432 a          679 c          714 g          439 t
ORIGIN      
        1 gagcacgcgg cggcttgtcc gcatcgcacg cgaacaacac gcgtcgatcc atgtgctgca
       61 tatctcgacg gcggaagaga tcgattttct ggcgcagcac aaggatgtcg ccagcgtcga
      121 agtcacgccg cagcatctga ctttgagcgc cgacgactat gcgcggcttg gcacgcggtt
      181 gcagatgaat ccgccgatcc gcgcgccgcg ccatcgtgaa cgcatctggt atgggctgca
      241 tcagggcatc gccgatattc tcggttccga tcacgcgccg catactttgg aagaaaaagc
      301 caaaccctat ccgcaaagtc catcgggcat gaccggcgtg cagacgctcg tgccgatcat
      361 gctcgatcat gtgaatgccg ggcggcttac gctcgagcgc ttcgtcgata tgacgagtgc
      421 agggccggcg cgactcttcg gcatcgccgg caaggggcgc atggccgtcg gctacgatgc
      481 cgatttcacg ctcgtcgatt tgaagcgccg cgagacgatc accgacgcat ggagcgcgtc
      541 acgcagcggc tggacgccct atgatggcgt cacggtcacg ggctggccgg tcggcactat
      601 cgtgcgaggg cagcgtatta tgtgggaggg cgagatcatg acatcagggc agggcgaagc
      661 cgtgcggttt attggcggtt tataaagaac aaacgcccgc aaaagcataa caagaccggg
      721 gcatcgcgtg ggaggacaag catatgcggt tcaatcgggc gctaatcccg gccctcctcg
      781 tttgggcttt ttcctttgct gctcaagccc agccggcacc gcaatccggg agtgacaagg
      841 cgccgacaat cctcgcctgt ttcaatgcgg ggcagacggc gattcgcgat ctccgtgcgt
      901 gtgccggagc gtggttgacg cccaaggccc tgctgctttg cgcgctccaa aacacggacg
      961 cgggaaaagc cgacgcgatc gcctgtccct ttttgccgga cacgcctggc ggacgcgcga
     1021 tcctggacgt tcttttggtg caacaatatt tgacgcccga gagcaatctg tccttggaca
     1081 cgcgcaactt ccccggtctg ccgggcgcgg ccgtggtgga cgtttgcaac aggacggccg
     1141 cgacgcgggg agactttctc gcttgcatcc aaacttcgac gagccaggct caaacccagg
     1201 cgctcttgca atgtatcaag acgagcaact ccgagggcgg ccgcgctttg tgtcttgcga
     1261 aaagcgcgac gggcaatcca gacctgccgg gcgccgtcaa atgtttcggc gtgcagggcg
     1321 gcgcgcccga taaatttgtg tcctgtctcg gcgcccctga tcgggtcgcc gcggcgaggg
     1381 cagcgatcgg atgcgccgca agcgggatct ccccatcggc tctcgccgat tgcattttac
     1441 cgagcgccag cccggccgaa aaagcgctcg cgtcctgcct cattcagtct caggacgacg
     1501 acagaaacgt cgctggatgc ctcggtcatt tttcgcctgc gatggggcgt tcggagcaag
     1561 cctcggcctg cttttccgat cccgggaata gcgccgcgaa atgcgcgcag gaacttttag
     1621 gcgacacaac gcatgtagcc gaaaccgtcg cggcctgtgc cgccacgcgg cgcaatgcgc
     1681 tggtcggttg ttttcttgat aagaggcccg agctggcggt gacgcgaaac ctctacactt
     1741 gtttcgcaga cggtggcgaa ggcagttcgc tcgttgcgag ttgcaccggc gaactcattc
     1801 cagacgagaa aatccgccgg acgcttgcct gcatcaccgc cgcgaacaat gatccagcgg
     1861 cacttgccgt ctgtgcttct gggtcggtct tgccgcgcga cgcggcgcat cccgtcgaat
     1921 gcgccgccac ctcgcaagat tcgccagttt ctttcgcgtt gtgtgcggcc gcgccgaaaa
     1981 cggatgacga ttggcggatt tccgcgcaat gcgcgatcgc gtcggcaggc gattccaaag
     2041 cctatgcggg ctgtatggga atgcctctcg ccatcaacga acttggcaag tgttttaagg
     2101 gcaaatttgg caaggattgt tttgggccaa caaactccat cgccaaaata ctcaatgtcg
     2161 cgcagtgagg ttaaaggcgg cgtgttggca tgacgggcac cggctggccg gtgggcagga
     2221 tcgtgcgcgg cagtcagatg attctggacg gggtggtaac gaat
//
LOCUS       DASWOG010000184         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_169121,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000184 DASWOG010000000
VERSION     DASWOG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169121"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB93_03755"
     CDS             complement(<1..236)
                     /locus_tag="VGB93_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145267.1"
                     /translation="MSRWVLALAVAALFGSASMGFAQQPPYDGAYPPLHVHGHHHWHS
                     GYYNRPCSIDQRDYAYINQCGELIPSIWDRTPVMG"
     gene            526..660
                     /gene="rpmH"
                     /locus_tag="VGB93_03760"
     CDS             526..660
                     /gene="rpmH"
                     /locus_tag="VGB93_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0145268.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRKVIATRRARGRKRLSA
                     "
     gene            708..1112
                     /gene="rnpA"
                     /locus_tag="VGB93_03765"
     CDS             708..1112
                     /gene="rnpA"
                     /locus_tag="VGB93_03765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0145269.1"
                     /translation="MQKSEIAGRERPPARLRTRAEFVRTAKGRRLRTPCFNLQMMART
                     ALSDPQPPRFGFTVTKKLGNAVLRNRIRHRLKEALRRGAALSGRPDHDYVILAQPAAL
                     TEDFAALQLQISQAIADIHAPPRKRRTPLMKD"
     gene            1123..>1634
                     /gene="yidC"
                     /locus_tag="VGB93_03770"
     CDS             1123..>1634
                     /gene="yidC"
                     /locus_tag="VGB93_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0145270.1"
                     /translation="MPQNNRNLVLAMALSILVIIGWNYFFAAPQYQKEHSAQLAQQQG
                     QPVQQAPGSPNALPPRSPNAPPQGGPANESPQAIMTREAALAASTRVPLDSTALFGSI
                     NLTGARIDDVSLKAYRETPNPKSPNIVLLSPSSAPTPYYAEMGYVSATAGLPLPGATT
                     VWKSDGGKLTS"
BASE COUNT          317 a          548 c          474 g          295 t
ORIGIN      
        1 cccataaccg gagtccggtc ccagatgctc gggatcagtt cgccgcactg attgatatac
       61 gcatagtcgc gctgatcgat cgagcaaggc cgattgtaat agcctgaatg ccaatgatga
      121 tggccgtgga cgtggagcgg cgggtaggca ccgtcatacg gcggttgctg ggcgaatccc
      181 atgctcgccg agccgaacaa cgcggccaca gccaaagcta aaacccacct ggacattgcg
      241 cgatccttcc accgaaaccc gtaacttggc aatgcattca atataggctt ggttcgctaa
      301 aaaggcattg cgcggcgccc gcacaaattg actttccggc cgggttgccc ttataaaccg
      361 cgcctggctg ttttaatgca gggccgactt tgagaagtct ttgatttttc agggccatgc
      421 ttgagggctg tgtcttcccc gcttgttggt ctcccgatcc ggcgttgccc ggcgggcgga
      481 ttccgcgttg atgacgcagc caaatattcg tgacggagcg aaaaagtgaa gcggacttat
      541 caacccagca agctggtgcg caagcgccgc cacggctttc gcgcccgtat ggcgacggtc
      601 ggcggccgca aagtgatcgc gacccggcgc gcacgcggtc gcaagcggct ctcggcctga
      661 gccgcgccgc tcctcaattt ttgaggcatg ccgcctcagg acggcccatg caaaagtccg
      721 agatcgccgg tcgcgaacgt ccgccagccc ggctgaggac gcgggccgag ttcgtccgca
      781 ccgccaaggg gcgtcgcttg cgcacgcctt gtttcaacct gcagatgatg gcccggacgg
      841 ccttatcaga cccacaaccg ccgcgtttcg gtttcacggt gacgaagaag ctcggcaatg
      901 ccgtgctgcg taaccgcatt cgccaccgcc tgaaagaagc cctgcggcgc ggggcggcgc
      961 tttccgggcg gccagaccat gattatgtaa tcctcgcgca gccggccgcg ctgaccgaag
     1021 atttcgccgc attgcagtta cagatcagcc aggccattgc cgacattcac gcgccgccgc
     1081 gcaagcgccg cacgcccttg atgaaggact agtttccgcg ccatgccgca aaataatcgc
     1141 aacctcgtct tggcgatggc cctctcgatc ctcgtgatca tcggctggaa ttatttcttc
     1201 gccgccccgc aatatcagaa agagcattcg gcccagctag ctcagcagca ggggcagccg
     1261 gtgcaacagg cgcccggctc gcccaacgcc cttccgccgc gctcacccaa cgccccaccg
     1321 cagggcggcc ctgcgaatga atcgccgcag gcgatcatga cccgcgaggc ggcactcgcg
     1381 gcaagcacgc gcgtgcctct cgacagcacg gctttattcg gctcgatcaa cctcaccggc
     1441 gcgcggatcg acgatgtttc gctcaaggcc tatcgcgaaa cgccgaatcc caagagcccg
     1501 aacatcgtcc tgctctcgcc ctcctccgca cccacgccct attatgcgga gatgggctat
     1561 gtcagcgcca ccgcgggtct gccactgccg ggcgcgacga ctgtctggaa atccgacggc
     1621 ggcaaactga cgtc
//
LOCUS       DASWOG010000185         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_316094,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000185 DASWOG010000000
VERSION     DASWOG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316094"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..569
                     /locus_tag="VGB93_03775"
     CDS             144..569
                     /locus_tag="VGB93_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145271.1"
                     /translation="MDEVDKVNPTHPRENPLAFLHSVLEQENASRFADEYLTIAMRAD
                     HAIWILTANNIVELPDSIVDRLTVFSISQPDSASLRSIIRGIYAATNARLNHAFVPEL
                     ERTIIETLFAYSPRAIGKLLRLAFGFAVSETGRISPSKI"
     gene            complement(755..830)
                     /locus_tag="VGB93_03780"
     tRNA            complement(755..830)
                     /locus_tag="VGB93_03780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(794..796),aa:Glu,seq:ctc)
     gene            complement(886..1734)
                     /locus_tag="VGB93_03785"
     CDS             complement(886..1734)
                     /locus_tag="VGB93_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145272.1"
                     /translation="MAKAEEEIASLSSEHGALSAALYDADGLYKTKQEQLAKLNQAQQ
                     ALSTLADQRLANHAAADTRAAGLELRLGDVSRTLVEAEQRFTERTAHAARLNDALVVA
                     KSNLEVLETNYAMLQRRFDAETARAAQLGKDLDELRQQRDADQGKLRALNIKIGVQDA
                     ALEDAKQRETRITAQRDQHVEKARESARALSEKYEGLRSESATLQGALDAARRRCEAL
                     ESELSQARAGRGVPSSGAEAEENASLRQEVSEIGAAIARLAPPALAADGEVRDANLEM
                     TNQTGT"
     gene            1762..2109
                     /locus_tag="VGB93_03790"
     CDS             1762..2109
                     /locus_tag="VGB93_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145273.1"
                     /translation="MMAFNRLFERKNFRGATAELGPICGMCLCHLAHSDFETAALDRK
                     FGPQMVSVGQNFFNGERNLRFQPAACQAQRAAPERRRKSKRHESCHQEPKRKDHCLFN
                     HAASPAGLSCTGL"
     gene            complement(2180..2776)
                     /locus_tag="VGB93_03795"
     CDS             complement(2180..2776)
                     /locus_tag="VGB93_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEY0145274.1"
                     /translation="MRPTSRREFSAKLALMAASGLALGLPKAALAVSEDDRPGEFTGD
                     ELMQAGHHFFGTLSRGLAQVVEEASRRWGRPNGYILGQEAGGAFIGGLRYGEGLMYTR
                     NAGDMKVFWQGPSLGFDAGADGDRTMMLVYGLPAVRAVFQRFAGLNGSAYFVGGFGMT
                     ALTANDVVVVPVRTGLGARLGVNVGYLKFTAHPTWNPF"
BASE COUNT          572 a          895 c          785 g          565 t
ORIGIN      
        1 gagcgaatag gccttcggca tcgcaaatca cctccgaacg gaagtgaatc acgagcctcc
       61 ctcaaaggga atccctcgat tcaatctgaa tgcccgacgc tctagctctc cttgaatcga
      121 aaacggctag cccgattttc cttttggacg aagtcgataa ggtcaacccg acgcaccccc
      181 gcgaaaatcc tctcgccttt ttgcattcag tactcgaaca agaaaacgcg agccggtttg
      241 cggatgaata tctgacgatc gcgatgcgtg ccgatcacgc gatctggatt ttgaccgcga
      301 ataatatcgt agaactgccg gattcgatcg tcgatcgcct gacggtcttc tcgatctccc
      361 agccagactc cgcttcgctt aggtccatca tccggggcat ctatgcggcg accaacgcac
      421 ggctcaacca cgcgtttgtg cctgaactcg agcggacaat tatcgagacg cttttcgctt
      481 acagcccgcg cgcaattggc aagctcctca gattggcgtt cggtttcgcc gtatcggaaa
      541 ccggacgcat ctcaccctcg aagatatagc gcgggctgcc gcgctcttag agaaatccgg
      601 cgcgaaaacg gcaattggat ttctatgagt cgaagttgaa aaaaaagggt tttggacgtc
      661 gccggacgtg ggtggacgtc catgcattgt caccaaaaac ttcccaaaac agccatcgag
      721 tccaggcgaa attcgtctaa ctcattgaaa tatttggcgc tccctagggg actcgaaccc
      781 ctgttttcgc cgtgagaggg cgacgtccta gaccgctaga cgaagggagc gggaagcgag
      841 gcttctagcg cgcacgcaac gcgtccgcaa gggcacaggg cattctcagg ttcccgtctg
      901 gtttgtcatt tccagatttg cgtcgcggac ttccccatct gccgcgaggg ccggaggcgc
      961 gagacgggcg atagctgcgc cgatttcgct cacttcctgg cgcagcgaag cgttttcttc
     1021 cgcctcggct cccgacgacg gcacgccacg gccggcgcgc gcttgcgaca attccgattc
     1081 aagcgcttca cagcgccgcc gcgccgcatc gagcgcgcct tgaagcgttg ccgattccga
     1141 tcgcagacct tcatattttt cgctcagcgc gcgcgctgac tcgcgcgcct tttcgacgtg
     1201 ctggtcgcgc tgtgccgtga tccgcgtttc acgctgcttg gcatcttcga gcgccgcatc
     1261 ctggacgcca atttttatgt tcagggctcg cagctttcct tggtcggcat cgcgctgctg
     1321 acgcaattca tcgaggtctt tgccgagttg agctgcgcgc gccgtctcgg catcgaatct
     1381 tcgctgcaac atcgcataat tggtctcaag cacttcgagg ttgcttttag caacgacgag
     1441 ggcatcatta agtcgtgccg catgtgcggt gcgctcggtg aaacgctgct cagcctcgac
     1501 cagggtacga ctgacatcgc caagccgaag ctcaaggccg gcggcacgcg tgtccgccgc
     1561 cgcatgattt gcaagcctct gatcggcgag cgtcgagagc gcctgttgcg cctgattgag
     1621 cttcgccaat tgttcctgtt tggtcttata gagcccgtca gcgtcataga gcgccgcaga
     1681 tagcgcccca tgttcgcttg agaggctggc gatttcctcc tcggctttcg ccaaagcctc
     1741 ggctctcccg cggacctctt cttgatggct ttcaaccgct tgtttgagcg caagaatttc
     1801 cgcggcgcga cggccgagct cggcccgatc tgcggcatgt gcctgtgcca cctcgcccac
     1861 tcggatttcg agacggcggc gctcgaccgc aaattcggcc cgcagatggt ctcggtcggc
     1921 cagaatttct tcaacggaga gcggaacttg cgtttccagc cggcggcgtg tcaggcgcag
     1981 cgcgcggcgc cagaacgccg gcgcaaaagc aagcgccatg agtcctgcca ccaggaaccc
     2041 aagcgcaaag atcattgcct gttcaatcac gcagcttcgc ccgccggatt gtcatgcacc
     2101 ggattatgac acctctgccc gagagatcaa aatcgacaca aatcggctgc ggacaggcta
     2161 acggcgcttt gagacgtctc taaaaggggt tccatgtcgg atgtgcggtg aacttcagat
     2221 atccgacatt cacgccaagt ctggcaccaa ggccggtgcg aacgggaacc acgaccacat
     2281 cattcgcggt cagcgcggtc atcccgaatc ctccgacaaa ataggccgag ccattgaggc
     2341 cggcaaagcg ctggaacacc gcacgcaccg ccggaagccc gtaaacgagc atcatcgtgc
     2401 ggtcgccgtc agcgccggca tcgaaaccga gggaaggccc ctgccaaaag accttcatgt
     2461 cgccggcatt gcgcgtgtac atcaggcctt cgccataacg cagaccgcca atgaaggcgc
     2521 cacccgcttc ctgcccgaga atatagccgt tcgggcggcc ccaacgccgc gaagcctctt
     2581 cgacaacctg ggccaggcca cgcgacagcg tgccgaagaa atgatgaccg gcctgcatga
     2641 gttcgtcgcc ggtgaattca cccggacgat cgtcctcgct cacagcgagc gcggccttcg
     2701 gcaagcccag ggccaagcca gaggccgcca tcaacgccaa tttcgctgaa aattcccgcc
     2761 gcgaagtggg tctcatggtc atctcccgaa tgagccggcg cgcggcatgg tcaatcc
//
LOCUS       DASWOG010000186         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_114395,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000186 DASWOG010000000
VERSION     DASWOG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114395"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..555
                     /gene="fae"
                     /locus_tag="VGB93_03800"
     CDS             46..555
                     /gene="fae"
                     /locus_tag="VGB93_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEY0145275.1"
                     /translation="MAKITKLGVGESLVGEGNEVAHIDLIIGPRGSVAESAFANALVN
                     NKDGFTTLLAVVAPNLLVKPNTILFNKVTIKGAKQAVQMFGPAQHAVAKAVADSVAEG
                     VIPVEEADDLFIAVGVFIHWDATDDKKIHDYNYQATKESILRAVTGEPKASEVVAKRN
                     TAKHPFSPE"
     gene            complement(601..1305)
                     /locus_tag="VGB93_03805"
     CDS             complement(601..1305)
                     /locus_tag="VGB93_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0145276.1"
                     /translation="MDIIPVIDLKGGAVVRARAGARAHYAPIETPLAPTSRPRDIVAG
                     FLALYPFRKIYIADLAAITGVGDHAAVIAELEDTFPDIEFWVDSGIATEAKAVNWLSQ
                     HRGALVIGSESLDDSETRPPQLRAESRRVLSLDFRGDVFQGPAQLAADAMLWPQRIIV
                     MTLAKVGTGSGPDFTCLKAIRAQAPKHEIYAAGGVRHGDDLRALAKAGIGGALVATAL
                     HDGKITKQDLRKYSAQ"
     gene            1386..>2077
                     /locus_tag="VGB93_03810"
     CDS             1386..>2077
                     /locus_tag="VGB93_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0145277.1"
                     /translation="MPGIIGWDIGGAHLKAARLENGMIVDVVQIACPLWLGLDHLHKA
                     FEEAKARLGSAKINVATMTGELSDAFADRTSGVLAISAAAVNALGPTLIYAGPAGFVA
                     ADIAAQNVATIASANWHASATFVARHIGEALFVDMGSTTTDIIPLKESKFVARGYSDA
                     TRMAHGELVYAGVVRSFLMAGLRLVPFGGHWVPIMNEAFATTSDVYRILGDLPADTDL
                     MPTADGQPKTIEA"
BASE COUNT          413 a          628 c          631 g          405 t
ORIGIN      
        1 cgttcggcgg gatgtctgtg ttcagttcca ccggaggaag tacatatggc caagataacc
       61 aaacttggtg ttggcgaatc gctcgtcggc gagggcaacg aggtcgcgca tatcgatctc
      121 atcatcggac cgcgcggtag cgtcgcggaa tcggccttcg ccaacgcgct cgtcaataac
      181 aaggacggat tcaccacgct gctcgccgtg gtcgccccga acctgctcgt caagcccaac
      241 acaatcctct tcaacaaggt caccatcaag ggcgccaagc aggccgttca gatgtttggc
      301 ccggcgcagc acgccgttgc caaggcggtc gccgatagcg tcgccgaggg cgtgatcccg
      361 gtcgaagaag ctgacgatct cttcatcgcc gtcggcgtgt tcatccattg ggatgccacc
      421 gacgacaaga agatccacga ctacaactat caggccacga aggaatcgat tctgcgcgcc
      481 gtcaccggcg agccgaaggc ttcggaagtc gttgccaagc gcaacaccgc caagcatccc
      541 ttctcgccgg aataatcggc tcgatctaga attcaaaagg ggagcttcgg ctcccctttt
      601 ttattgtgcg ctgtacttgc gcagatcctg cttggtaatc ttgccgtcgt gcagcgccgt
      661 ggcgacgagc gcgccgccaa ttccggcctt tgccagtgcc cgcaggtcat cgccatgacg
      721 aacgccgcct gcggcgtaaa tttcgtgctt aggcgcctgt gcgcgaatcg ctttgaggca
      781 cgtgaaatcc ggcccgctac ccgtgccgac cttggccaag gtcatgacga tgatgcgttg
      841 aggccaaagc atcgcatcgg ccgccaattg ggctggcccc tgaaagacat cgccacggaa
      901 atcgagtgac agcacgcgcc gcgattcggc tcgaagctgc ggaggacgcg tctcggaatc
      961 gtccaggctc tcgctgccga taacgagcgc accgcggtgc tgactaagcc agttaacggc
     1021 tttggcctca gtcgcaattc ccgaatcgac ccagaattcg atgtccggaa atgtgtcctc
     1081 aagttcggca atgacggcgg catggtcccc aacgccggta attgcagcga gatcggcgat
     1141 gtagatttta cggaacggat aaagcgcgag aaagcccgcg acgatatcgc gcggccggct
     1201 cgttggcgcg agcggcgtct caatcggggc ataatgcgcc cgcgccccgg cgcgcgcgcg
     1261 caccaccgcg ccacctttca gatcgatcac tgggataatg tccatgccgc ctcgtatacg
     1321 cgcggctttc gcgcctcagg cgccgcgtga tagagcctgc gtaacgtaat ttcagggcag
     1381 ttcaaatgcc gggcattatc ggctgggaca tcggtggcgc gcatttgaaa gcggcgcggc
     1441 ttgaaaacgg catgatcgtc gatgtcgtgc agatcgcctg tccgctgtgg ctgggtctcg
     1501 atcacttaca caaagctttt gaagaggcca aggcgcggct cggctccgca aagatcaacg
     1561 tcgcgacgat gaccggcgaa ttgtccgacg cctttgccga tcgcacctcc ggcgtcttgg
     1621 cgatttcggc ggcggcggtg aacgccctcg gtccgacgtt gatttatgcc gggccggcag
     1681 gtttcgtcgc ggcagatata gcggcgcaga atgtcgctac gattgcctcc gcaaactggc
     1741 acgcgagcgc gacgtttgtg gccagacata ttggcgaggc gctctttgtc gatatgggtt
     1801 cgacgacgac cgacatcatt ccgctgaaag aaagcaaatt cgtggcgcgg ggctatagcg
     1861 acgcaacgcg catggcgcat ggcgaactgg tctatgccgg tgtcgtgcgc agtttcctga
     1921 tggccgggct gcggctcgtg ccgttcggcg gacactgggt gccgatcatg aacgaggcgt
     1981 ttgccacgac gagcgacgtc tatcgtattc tcggtgatct tccggcagat accgatctca
     2041 tgccgacagc cgatggtcag ccgaaaacga tcgaagc
//
LOCUS       DASWOG010000187         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_261068,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000187 DASWOG010000000
VERSION     DASWOG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261068"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VGB93_03815"
     CDS             complement(<1..1974)
                     /locus_tag="VGB93_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145278.1"
                     /translation="MGGALAEAKTDMHVDADSAAAQLQGAVNQALACLGAGETAKACA
                     HLDEVQHFALASDTASHVFGLFYFNTGDAASALAWFEQALRLNPSHLEASKGRGVALQ
                     RLGRAPEVIACFQAIARDNPADAEAAHMSGVLLQNLGKVDAALAAYDRALRIKPDYCE
                     TLRNRGALLEQAVRLDEALHDFDALIALRPGEAINYFNRGCVLQKMQRYEAALGDHEA
                     AARRNANLPENEVNRGNVLQKLGRHMEAASCYDSAIHLRPHYPQAYYNRGIARQKIGH
                     LFEAISDFDVALAQKPNYPEAFCNRGNALSEMGRLNEAVASYEHALALRPDFRQAQVN
                     RANVLFALGRAEMARSAAAEILTQDPAHPQALCISGAAHQRLGDLETALAFLDRAVAA
                     RPDYAEAWLNRGNVLQEQDRLDDALASYEKALVLRPAYPEVLSGRGVALKEFGRLDEA
                     MQAFDAALRLKPNYPDARNNRAGALLSAGDLVQGFAAFESRWERSNAPPKTLISALPH
                     WRGEPLKGRHILVWDEQGFGDLIQFCRYLPLLTAQGADVALFGRKSMFRLLSTLPAPP
                     RFVDNAPAQDGFDYQSALMSLPLAFGTSLATVPASMPYLHAEPARVAVWADRLGRHGF
                     RIGICRQGNLKINLKRNMPLACFAELAAIPGVRL"
     gene            2121..3221
                     /gene="hrcA"
                     /locus_tag="VGB93_03820"
     CDS             2121..3221
                     /gene="hrcA"
                     /locus_tag="VGB93_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0145279.1"
                     /translation="MTERFDPSRNALASDSSAFGLLNERSREIFRHIVDSYLTYGGPV
                     GSRQLSRLLPMSLSPASVRNVMQDLEELGLIYARHISAGRLPTERGLRFFVDAMLEIG
                     DLGQDERAQIEAQVRASSKEHALDAVLSDAMSMLSGLTGSAGVVVTTKENARLKHIEF
                     VRLDPERALAVLVAADGSIENRVVQTPRDLPASALIEAGNYLNMRIAGRTIIETKAEI
                     EVGVAAAEAELGELTARLVAAGYASWIETSTQGQQLIVRGQANLLDNLTALEDLERIR
                     LLFADLETQKDVIDLLSRAEGGEGVRIFIGSENKLFSLSGSSMIAAPFRDGQQRVVGV
                     LGVIGPTRLNYARIVPMVDYTSKIIGRLVHGS"
     gene            complement(3327..4368)
                     /locus_tag="VGB93_03825"
                     /pseudo
     CDS             complement(3327..4368)
                     /locus_tag="VGB93_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173941.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
BASE COUNT          813 a         1325 c         1395 g          835 t
ORIGIN      
        1 caagcggacg cccgggatcg cggccaattc cgcgaaacag gcgagcggca tgttgcgttt
       61 cagattgatc ttgagattgc cctgccggca gatgccgatg cgaaagccgt ggcgcccgag
      121 gcgatctgcc caaacggcaa cccgcgctgg ttcggcatga agatagggca tgctggccgg
      181 cactgtcgcg agcgatgtgc cgaaggcgag cggcaggctc atcaaagcgc tttgatagtc
      241 aaagccgtcc tgtgctggcg cattatcgac gaagcgcggc ggcgccggca gggtcgaaag
      301 cagccggaac atcgacttgc ggccgaaaag cgcgacgtcc gcaccttgcg ccgtcaacag
      361 cggcaggtag cggcagaatt gaatgagatc gccaaatcct tgttcgtccc aaacgagaat
      421 atgccgtccc ttgagcggct caccgcgcca gtgtggcaat gctgagatga gtgttttcgg
      481 cggggcgttg ctgcgctccc agcggctttc gaaagccgcg aacccttgca cgagatcgcc
      541 cgcgctgagc agcgctccgg cgcgattgtt gcgcgcgtcg ggataattgg gtttcagccg
      601 caatgccgca tcgaaggctt gcatcgcttc gtcaagccgg ccaaattctt tcagcgcgac
      661 accgcgaccg gacaagacct ccggataggc cgggcgcaat accagcgcct tttcgtagct
      721 tgcgagagcg tcgtcgaggc ggtcctgctc ctgaaggacg ttgccgcgat taagccaggc
      781 ctcggcataa tccgggcgtg ccgcgacagc gcgatcgagg aaggcgagcg cggtctcgag
      841 gtcgccgaga cgctggtgtg cggcgccgga aatgcacaac gcttgtggat gggccgggtc
      901 ctgcgtcaaa atctcggcgg cggcgctgcg cgccatctcg gcgcggccga gggcaaagag
      961 cacattggcg cgattgacct gcgcctgacg aaagtccggc cgtaatgcga gcgcgtgctc
     1021 gtaagacgcg accgcttcgt tcaatcgccc catttcgctg agcgcattgc cgcgattgca
     1081 aaaggcttct ggatagttcg gcttctgcgc gagggccaca tcgaaatcgc tgatcgcctc
     1141 gaacaggtgg ccgatctttt gccgggcgat gccacgatta taataagcct gcggataatg
     1201 aggccgtaga tgaattgcgc tgtcgtaaca gctcgcggct tccatgtgcc gcccgagctt
     1261 ttgcagaaca ttgccgcgat tgacttcgtt ttcgggcaga ttggcgttgc gacgcgctgc
     1321 ggcttcgtga tcgcccagtg ccgcctcgta gcgctgcatc ttttgcaaga cgcagccgcg
     1381 attgaaatag ttgatggctt cgcctggcct gagcgcgatc aaggcatcga agtcgtgcag
     1441 agcctcgtcg aggcgcactg cctgttcaag caaggcaccg cggttgcgca gcgtctcgca
     1501 ataatctggc tttatccgca gcgcgcgatc ataggcggcc agcgccgcgt ccaccttgcc
     1561 gaggttttgc agcaggacgc cgctcatatg cgcggcctcg gcatcggcgg gattgtcgcg
     1621 cgcgattgcc tggaaacagg cgatcacctc gggcgcacgc ccaagtcttt gcagcgcgac
     1681 cccgcggccc ttgctggcct ctagatgaga ggggttgagc cgtagcgcct gctcgaacca
     1741 ggcgagggct gaggcggcat cgccggtgtt gaaatagaac agaccgaaca catggctagc
     1801 ggtgtcgctt gccagggcga aatgctgtac ctcatcgagg tgggcacagg ctttcgcggt
     1861 ttcgccggcg ccgagacagg ccagagcctg attcacggcg ccctgaagct gcgccgcggc
     1921 gctatcggcg tccacatgca tgtccgtctt ggcttcggcc agtgctccgc ccatggaaat
     1981 gcctttgctc ttgaagcagt ggcagtgaat ctgaacaatc tttcccgggg ctgacgccga
     2041 ccgcgcggct tgcgtccggg ccttgggccg gccatcttta aggtcgagga agatgcaggc
     2101 gggcgtggca ataaaggcag atgacggaac ggtttgaccc ttcgcgcaat gcgctcgcat
     2161 ccgacagcag cgcttttggc ctgctgaatg agcgctcgcg cgagattttc cgccatatcg
     2221 ttgattccta tctgacctat ggcggcccgg tcgggtcgcg gcagctttca cgtcttttgc
     2281 cgatgtcgct gtcgccggcc tcggtccgca atgtcatgca ggaccttgag gaactcgggc
     2341 tgatctacgc ccgccacatc agcgccggtc gtctgccgac agagcgcggt ttgcgatttt
     2401 tcgtcgatgc tatgctcgaa atcggcgatc tcgggcagga cgaacgcgcg cagatcgaag
     2461 cgcaagtgcg agcctcgtcc aaggaacatg cgctcgacgc tgtgctgagc gatgcgatga
     2521 gcatgttgtc gggcctgacg ggcagcgccg gcgttgtcgt cacgaccaaa gagaacgcac
     2581 ggctcaaaca tatcgaattc gtccgcctcg atcccgagcg cgccttggcc gtgctcgtcg
     2641 ctgccgacgg ctcgattgag aaccgcgtcg tccagacgcc acgcgatctg ccggcgtcgg
     2701 cgctgatcga ggctggcaat tatctgaaca tgcgcatcgc cgggcgcacc atcattgaaa
     2761 ccaaggccga gatcgaagtg ggcgttgccg cggcggaggc cgaactcggc gaactgacgg
     2821 cacggctggt cgccgccggc tatgcgagct ggatcgagac aagtacgcag gggcaacagc
     2881 tcatcgtccg tggtcaggcc aatctgctcg acaatctcac cgcgctcgaa gatctcgaac
     2941 gcattcggct gctttttgcc gatctcgaaa cccagaaaga tgtcatcgat ctcttgagcc
     3001 gcgccgaggg cggcgaaggc gtgcgcatct tcatcggctc agagaacaag ctattctcgt
     3061 tgtccggttc atcgatgatc gcggcacctt tccgcgacgg tcagcagcgt gtcgtcggcg
     3121 tgctcggtgt catcggtccg acacggctca actacgcgcg cattgtgccg atggtcgatt
     3181 atacgtcgaa gatcatcggc cgcctggtgc atggatcgta ggacagcgcg tcatggccgg
     3241 gcttgacccg gccatccaaa cacacagcta tttcatgaag ttggcgcttg gatgcgcgga
     3301 tcaagtccgc gcatgacggc acgctgtcat tcttcgttct tcttcagcaa cgttttcaac
     3361 ttgtagagcg cggcctgggc gtcgcgcggc gaaagaccat cagggtcgat agcgtcgagt
     3421 gcttcttcga tttcgctcgg cgtgggcacc ggaggcggcg gcggcgcggc ggcgaagagc
     3481 ggcaggtcgg caatcggcct gaccgtcttg cgccgatcgc cggcctcgaa ttcagcgagc
     3541 agcgtttgcg cgcgcttcac cacggcgggc ggcagaccgg cgagcttttc cacttaaatg
     3601 ccataggagc ggtctgccgc acccgcgatg atttcgtgca gaaagacgac atcgcccttc
     3661 cagtcggcaa cgcgcatggt cagattgacg acgcgcgaga gctgtttggc aagatgtgtc
     3721 aattcgtgaa aatgcgtcgc gaaaagcgcg cgcgatttat tcttgccgtg caattgctcc
     3781 atcacagccc aggcgatggc gagaccgtcg aacgtcgccg tgccgcgccc gatctcgtcg
     3841 agaatgacga gcgagcgctc cgtcgcttga ttgaggatcg cagccgtctc gaccatttcg
     3901 accatgaagg tcgaacggcc gcgggcgaga tcgtccgccg cgccgacgcg cgaaaagaga
     3961 cggtcgacaa tgccgatctt gcaatgcttt gccgggacga acgagcccat ctgcgccaag
     4021 gcggcgatca gcgcgttctg ccgcagatac gtcgatttgc cggccatgtt cgggccagtg
     4081 atcacggcga tgagccccgg tgcacttccg gagcccgaga gattgcaatc attagcgacg
     4141 aagggtttgc ccccgcgtgc aagcgcggct tcgacgacgg gatggcgtcc ggcttcgatg
     4201 acgaaattca gcgaagtatc gatgtccggc cgcgtccagc cgcacacctc ggcgagatcg
     4261 gccagcgccg ccgcgacatc gagtgccgcc agcgcctcgg ccacggcttt gagcacaggt
     4321 tctgcgtcga gaatttgcgc actaagcttg tcgaaaactc ccaattcg
//
LOCUS       DASWOG010000188         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_352462,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000188 DASWOG010000000
VERSION     DASWOG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352462"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGB93_03830"
     CDS             complement(<1..399)
                     /locus_tag="VGB93_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEY0145280.1"
                     /translation="MKFPHNPGLWLKRHGAPALLGASLISLLFAAPVLADGHASPGQM
                     GMLTPVTPIAEEIHFFYNDILTPIIFAVSIFVGALLLYVVYRFNEKANPTPSVLTHHT
                     GLEVAWTIAPVLILVFIAIPSFRLLDHQLVI"
     gene            792..1322
                     /locus_tag="VGB93_03835"
     CDS             792..1322
                     /locus_tag="VGB93_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0145281.1"
                     /translation="MTDLTGLRRVAAWVPLLCLLAIFVGNFAVPTALAQGVVKAKHGD
                     WEVRCETPPGAAHEQCALLQSVAAEDRPNINLVVIVLKTADAKSKLLRVIAPLGVLLP
                     SGLGLKIDNIDVGRAGFVRCLPTGCVAEVVMDDKLIGEMRNGHNATFIIFQTPEEGIG
                     IPLALDGFGEGYDALP"
     gene            1438..2142
                     /locus_tag="VGB93_03840"
     CDS             1438..2142
                     /locus_tag="VGB93_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145282.1"
                     /translation="MQKLAQGNAAVGTARHRALAGSMVFLGSALLALTGCSKTVGSFD
                     TDDSNQTKMSNLWALVQFKPIPSQPQPFAPIKCPEISVQDGTAEDRIYGSGDDRSNAN
                     VRYQFSIINFARDCQIVGNQYQMKVGIEGRVLLGPLGSPATYPAPVRVAVVSRADASP
                     TVSKLYRVPASVAPGQTEGPFTIVTDPLSVPVTGKYSDQDYIIKIGFDSQANGDVGAP
                     RAHHRHHPAAAAAGPN"
     gene            2149..3132
                     /gene="dgcA"
                     /locus_tag="VGB93_03845"
     CDS             2149..3132
                     /gene="dgcA"
                     /locus_tag="VGB93_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HEY0145283.1"
                     /translation="MPKRGLHIAIERFPIAGRFVTSRDAKAEAVVVVVTLKEGDFRGR
                     GECVPYARYGESIESVIAQIEGWRSPIDDGAGRDDLQKALPAGAARNALDCAFWDLEA
                     KRSGVRAFERAGLAAPPPVITAYTLSLDTPEAMAMAAKSAARPLLKLKLGGDGDVQRL
                     AAIRDAAPDAALIVDANEAWSPENLAANLAACATHGIALIEQPLPAGYDAILADILHS
                     VPFCADESLHDRADLAALRGRYYAINIKLDKAGGLTEALALADEAAQLGFEIMIGCMV
                     ATSLAMAPALLLAGRAQYVDLDGPLLLARDRPEGLVYDGSRIHPPAPVLWG"
     gene            3199..4047
                     /locus_tag="VGB93_03850"
     CDS             3199..4047
                     /locus_tag="VGB93_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY0145284.1"
                     /translation="MLDNGEQSLRARLSGLFDDDAPRTLAVRVFNIALATLIIVNVIA
                     VILESVSWMSQRYSITFLAIERVATAIFVAEYFLRVWTAVDYRNGRFRNPIWGRLEYM
                     RGFFPLIDLIAVLPAVLGFFGGADLRVLRLLRLLRMIKLTRHSTVFGLLWAVLRQEAR
                     AIAALIFVLCLTLTVSGALMYMIEGDVQPNVFSSIPASMWWAIETVTTVGYGDMVPVT
                     FAGRVLGGMVSIIGIGTLALFSGLITVGYLNELRLRRTPKHMDGDELTCPHCGGLLRH
                     SRAKTS"
     gene            complement(4050..4667)
                     /locus_tag="VGB93_03855"
     CDS             complement(4050..4667)
                     /locus_tag="VGB93_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0145285.1"
                     /translation="MGIEGRDKAKALEAGALSVSGQLGKTVLRLRKSFNLSLSELSEQ
                     SGVAKSIISQIERNETNPTLATIWRLAQALDVSIEGMLQVSEDEPFLEKSTRGDTPIL
                     VSDDGKCRLEIFGWIKTVEWLQWYGFHAEAGGALASDPHQRGSVECLSVTDGELEVEV
                     AGTVEHAKAGETLRYRCDRPHVIRNRTGKPAAAMMVCILKAAAME"
     gene            complement(4747..4944)
                     /locus_tag="VGB93_03860"
     CDS             complement(4747..4944)
                     /locus_tag="VGB93_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145286.1"
                     /translation="MQLLLGIIVGIFLTIGAAYVMDSGKSPVCPAGAGCPVVNWDEAG
                     ARFAHFKEDVSSGWHRLTGHS"
     gene            complement(5030..5350)
                     /locus_tag="VGB93_03865"
     CDS             complement(5030..5350)
                     /locus_tag="VGB93_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145287.1"
                     /translation="MSQTLEILAGNMRADGVDEHYGRDVETQARAGGGLLRGAPVLKS
                     PPSAPDIKPGLSRPPEHLAAPALPTAITPPPVAPVSAAPRGEPGALTRLDRLSTSEKV
                     ALFS"
     gene            5363..>5576
                     /locus_tag="VGB93_03870"
     CDS             5363..>5576
                     /locus_tag="VGB93_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145288.1"
                     /translation="MNEKPDRRIVREVLLAHRLFEFGEANSIFFISIFVSLFGEEVFK
                     VVVEDISEEGFEDCDAEVGVEDNMEVD"
BASE COUNT         1058 a         1708 c         1672 g         1138 t
ORIGIN      
        1 aatcaccaat tgatgatcga gcaggcggaa tgacgggatc gcgataaaga cgaggatgag
       61 caccggcgcg atcgtccacg cgacttcaag gcccgtgtgg tgggtcagca ccgaaggcgt
      121 cggattagct ttctcgttga agcggtagac cacatagagg agcagcgcgc cgacgaaaat
      181 gctgaccgca aaaatgatcg gcgtcagaat atcgttgtaa aagaagtgga tttcttcggc
      241 gatcggtgtc accggcgtca gcatccccat ttgccccggg gacgcatggc catcggccaa
      301 aaccggcgcg gcaaagagca gggaaattaa ggaggcaccc aaaagcgcgg gcgcgccatg
      361 acgttttagc caaaggcccg gattatgcgg aaatttcatg aattttcgtc gctcccctgc
      421 ccaccactta tcgcaagtcc tcgccaccgg ccccgcggcg cgagcgtgtg ggttgaactg
      481 ttcaaaacca caaagattta tttcgcgcaa ctcgactttt cgcaagccat tacgcaaatt
      541 tgccacatcg aggccgcttt gggcatttcg cccctccatg cggcggcgca gcatacagcg
      601 cgaccgaaca ggggtggttg tatcccaagt cacctggcct tagccctgcc ggatttgcat
      661 ccctcttatg atccgctaat gaaacattcc ggtttgcgct gcggtctcga ttcgattgcg
      721 gcgcaagcgg tgccgccacg ttctgcagac gcagcgtcgc tttggatttg ccgattgcac
      781 gagaacagag catgaccgac ttgacgggat tacgccgtgt ggccgcctgg gtgccgcttc
      841 tctgtctcct cgcaatcttt gttggtaact tcgcggtgcc cacggctctg gcccagggtg
      901 ttgtcaaagc caagcatggc gattgggaag tccgttgcga gacgccgcca ggcgccgccc
      961 atgagcaatg cgctttgttg caaagcgtcg ccgccgagga tcggccaaac atcaatctgg
     1021 tcgtgatcgt cctcaagacg gcggatgcga agagcaagct tttacgcgtg atcgcgccgc
     1081 tcggtgtcct gctaccctcc ggcctggggc tcaagatcga caatatcgat gtcgggcggg
     1141 ccggattcgt ccgttgccta ccgacaggct gcgtcgccga ggtcgttatg gatgacaagc
     1201 ttatcggcga gatgcgcaac ggccataatg cgaccttcat catcttccag accccggaag
     1261 aaggcattgg cattccgctg gcgctggacg gcttcggcga ggggtacgac gcgttgccgt
     1321 aggagggcgc gccggcagat cagcctgaat ctggaaagtt gcggactttc aagaaaagtt
     1381 tatgccgtaa attttacgtg cgcacagcgc gttacggaga agattggagc gggggacatg
     1441 cagaagcttg cgcagggcaa tgcggcggtt gggacagcgc gccatcgggc gctggcaggc
     1501 agcatggtgt ttctgggcag cgccttgctc gctctcactg gctgcagcaa gaccgtcggc
     1561 agtttcgaca cggacgacag caaccagacc aagatgagca acctctgggc gctcgtccaa
     1621 ttcaagccca ttccatcgca gccgcagccc ttcgccccga tcaaatgccc ggagatcagt
     1681 gttcaagacg gcacggccga ggaccgcatc tatggctctg gcgacgaccg ctccaacgcc
     1741 aacgtccgct accaattctc gatcatcaac ttcgcccgtg actgccagat cgtcggcaat
     1801 caatatcaaa tgaaagtcgg tattgaaggc cgcgtgctat tagggccgct gggctcaccg
     1861 gcgacctacc cggccccggt gcgtgtcgct gtcgtcagcc gcgccgacgc gtcgccgacc
     1921 gtcagcaagc tctatcgcgt gcccgccagc gtcgcgcccg gccagactga aggtcccttc
     1981 acgatcgtga ccgacccgct gagcgtgccg gtcaccggca aatattccga ccaggattac
     2041 atcatcaaga tcggtttcga ttcgcaggcc aacggcgacg ttggcgcacc gcgcgcgcat
     2101 caccggcatc atccggccgc ggcggctgcc ggacctaact gaactatcgt gcccaagcgc
     2161 ggcttgcaca tcgcgatcga gcgctttccg atcgccggcc gatttgtcac ctcgcgcgac
     2221 gccaaggccg aagctgtcgt cgttgttgtg accttgaagg agggcgattt ccgcggccgt
     2281 ggcgaatgcg ttccctacgc gcgctatggt gaaagcatcg agagtgtcat tgcgcagatc
     2341 gaaggctggc gttccccgat cgacgacggc gccggacgcg acgatttgca aaaggctttg
     2401 cccgctggcg cagcgcgcaa tgcactcgat tgcgcgttct gggatctaga agcaaagcgc
     2461 agcggggtgc gcgctttcga acgcgccggc ctcgcggcgc cgcccccggt gatcaccgcc
     2521 tatacgctct cgctcgatac gcccgaggcg atggcaatgg cagccaaatc tgccgcacgc
     2581 cccttgctga aactcaagtt gggcggcgac ggcgatgtcc aacggcttgc cgccattcgc
     2641 gacgccgctc cggacgccgc actcatcgtc gatgccaacg aagcctggtc gccggaaaat
     2701 ctcgcggcca atctcgccgc ctgcgcaacg cacgggatag cgctgatcga acagccgctg
     2761 cccgcagggt acgacgccat cctcgcagac atcctgcact ccgttccatt ttgcgccgac
     2821 gaaagcctgc acgaccgcgc cgatctcgcc gccctgcgcg gccgctatta tgcgatcaat
     2881 atcaaactcg acaaggcagg cggcctcact gaagcgctgg cgctcgccga cgaggcggcg
     2941 cagctcggct tcgaaattat gatcggctgc atggtcgcga catctctggc gatggcgccg
     3001 gccttgctgc ttgcggggcg cgcgcaatat gtcgacctcg atgggccgct gcttctcgca
     3061 cgcgaccggc cagaaggtct tgtttatgac ggctcgcgga ttcatccgcc cgcgcccgtc
     3121 ctgtggggat gacgcgcgct gcaaaattgc ttcatagtgc tcagctagcg atgttcccac
     3181 gatgtcagga cgggacggat gttggataac ggcgagcaat ctttacgcgc ccgcctgagt
     3241 gggcttttcg acgatgacgc gccgcgcacc ctggcggttc gcgtcttcaa tattgcactt
     3301 gcgactctca tcatcgtcaa tgtcattgcc gtcatcctcg aatccgtctc atggatgagt
     3361 cagcgctatt ccattacctt tctggcgatc gagcgtgtcg cgaccgcaat ctttgtagcc
     3421 gaatattttt tgcgcgtctg gaccgccgtc gattatcgca acgggcgttt ccgcaatcca
     3481 atctggggcc gcctggaata tatgcgcggt ttctttccgt tgatcgatct gatcgccgtt
     3541 ctgccggcag tgctcggctt ttttggcggc gccgacctgc gcgtcctgcg gctgttgcgg
     3601 ctgttgcgga tgatcaagct gacgcggcat tcgaccgtct tcggcttgct gtgggcggtg
     3661 ctacgccaag aggcacgcgc catcgccgcg ctgatcttcg tcctctgcct cacgctgacc
     3721 gtcagcggcg cgctgatgta catgatcgag ggcgacgtgc agccgaacgt tttctcgtcg
     3781 attcccgcct cgatgtggtg ggcgatcgaa acggtgacga ccgtcggtta tggcgacatg
     3841 gtgccggtga cgtttgcggg gcgcgtcctc ggcggcatgg tttcaatcat cggtatcggt
     3901 acgctggcgc tgttttccgg cctcatcacc gttggctatc tcaacgagtt gcgtctgcgg
     3961 cggacaccga aacatatgga tggcgatgaa ctcacctgcc cgcattgcgg cggcctgctc
     4021 cggcattcgc gcgccaaaac aagctaaagt tactccatcg ccgcggcttt gaggatgcag
     4081 accatcatcg ctgccgccgg tttgcccgtc cgattgcgaa tgacatgcgg ccggtcgcag
     4141 cgatagcgca atgtctcgcc ggccttggca tgttcgacgg tccctgcgac ttcgacttca
     4201 agctcgccat ccgtgaccga cagacattcg accgagccgc gctgatgcgg atcggacgcg
     4261 agcgcgccgc cggcctcggc gtgaaagccg taccattgca gccattcgac ggttttgatc
     4321 cagccaaaga tttcgagccg gcatttaccg tcgtcggaca ccaggatcgg cgtgtcgccg
     4381 cgcgtcgact tttcgagaaa cggttcgtct tcgctgacct gcaacatgcc ttcgatcgac
     4441 acatcgagcg cctgcgccaa tcgccagatg gtggcgagtg tcggattggt ctcgttgcgc
     4501 tcgatctggc tgatgatcga cttggcgacg ccggattgtt cggaaagctc cgaaagcgaa
     4561 agattgaagg atttacgcag gcgcagaaca gtcttgccaa gctggccaga aacggaaagc
     4621 gcgcccgcct ccagtgcctt cgccttgtcg cgaccctcga tgcccaatac ccgcctccgg
     4681 aaccttcaat cagtcccgga tattaaacga ttccgtttga aatatcgaac gcctcttcgc
     4741 ccaggctcac gaatgcccgg tcagccggtg ccagccgctg gaaacatctt ctttgaaatg
     4801 tgcgaagcgc gcgccggctt cgtcccagtt gacgaccggg cagccggcgc ccgcgggaca
     4861 caccggcgac ttgcccgaat ccatcacata ggcggcgccg atggtcagaa aaatcccgac
     4921 gataattcct agcaagagct gcatcgcatc ctccctatgt tgatgccgac tctgcgcggc
     4981 cgctccaaaa acttaacggc ggcggcaaag ttccggctga aaatcatgtt taactgaaga
     5041 gggcgacttt ctccgatgtc gaaagccggt cgagacgtgt caacgcaccc ggctcgccgc
     5101 gcggcgcggc ggacacgggt gccacgggcg gtggggtgat tgcggttggc agcgctggcg
     5161 ccgccaagtg ttcgggtggc cgcgaaagac ccggcttgat gtccggcgcg gacggcggcg
     5221 acttgagcac gggcgcgcca cgcaacaggc cgccgccggc gcgcgcctgc gtttcgacat
     5281 cgcggccata atgctcatcg accccatcgg cgcgcatatt cccggcgagg atctcaagcg
     5341 tctgcgacac gtcgcgcagc ctttgaacga gaagccggat cgacggatcg ttcgggaggt
     5401 gttgctcgcg catcgtctgt tcgagtttgg cgaggccaat tcaatatttt tcatttccat
     5461 ttttgtttca cttttcggtg aagaagtttt caaagttgtg gttgaggata tttctgagga
     5521 aggttttgaa gattgtgatg cggaagttgg tgtggaagat aatatggaag ttgatg
//
LOCUS       DASWOG010000189         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_261089,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000189 DASWOG010000000
VERSION     DASWOG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261089"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGB93_03875"
     CDS             complement(<1..377)
                     /locus_tag="VGB93_03875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEY0145289.1"
                     /translation="MLDDKDRIFLNLYGFGDWRLKGAQARGGWDNTKTIVDKGKDWVI
                     NEMKASGLRGRGGAGFPTGLKWSFMPKPDPARPSYLVVNADESEPGTCKDREILRNDP
                     HLLIEGCLLASFAMQAHVCYIYVR"
     gene            complement(386..1054)
                     /gene="nuoE"
                     /locus_tag="VGB93_03880"
     CDS             complement(386..1054)
                     /gene="nuoE"
                     /locus_tag="VGB93_03880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0145290.1"
                     /translation="MSVRRLAEIQPESFAFTPENEARCGREISKYPPGRQASAVIALL
                     WIAQKQNDGWLPKPAIENVAAKLGMATIRVLEVATFYTMFNLAPVGKYFIQFCGTTPC
                     VLRGADAIKKVLQARIGAQSHVTADGLFSWLEVECLGACCNAPMVQINDDYYEDLTEE
                     NFNKLLDDLAAGRPVKVGSQTGRKTSEPAGGLTSLTSLYGPDGLSGPQSDPVVQSAAP
                     ANEN"
     gene            complement(1051..2238)
                     /locus_tag="VGB93_03885"
     CDS             complement(1051..2238)
                     /locus_tag="VGB93_03885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0145291.1"
                     /translation="MTDQNVRNFSINFGPQHPAAHGVLRLVLELDGEIVERVDPHIGL
                     LHRGTEKLMEARTYLQNVPYFDRLDYVAPMNQEHAFCLAIERLLGVEVPRRAQLIRVL
                     YAEIGRILSHLLNVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHAAYFRPGG
                     VHQDLPPRLIEDIGAWCDPFLKVLDDLQELFIENRIFKQRNVDIGVVSLEDCWRWGFS
                     GVMVRGSGAAWDLRKSQPYECYEEMEFDIPVGLHGDNYDRQVIRMEEMRQSTSIMKQC
                     VEKLMSPNGQGPINVKGKVSPPSREEMKHSMEALIHHFKLYTEGVHVPAGEVYAAVEA
                     PKGEFGVYLVSDGTNKPYRCKIRAPGFAHLQAMDFLCRKTMLADVSAILGSLDIVFGE
                     VDR"
     gene            complement(2235..2876)
                     /locus_tag="VGB93_03890"
     CDS             complement(2235..2876)
                     /locus_tag="VGB93_03890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0145292.1"
                     /translation="MSDELQALGNSIAAALPGAIAASVVAYDELTLDVEPARLIETVR
                     YLRDDPSCLFISFIDLTAVDYPTRVPRFDVVTHLLSPKHNRRIRIKVATDEDVPVASL
                     TALYPAADWYEREAYDLFGVFFSGHPDLRRILTDYGFEGHPLRKDFPMTGYVEVRYDD
                     AQKRVVYEPVNLRQEFRSFDFLSPWEGAVDYVLPGDEKASTVPPVQDPQVKPQ"
     gene            complement(2889..3458)
                     /locus_tag="VGB93_03895"
     CDS             complement(2889..3458)
                     /locus_tag="VGB93_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0145293.1"
                     /translation="MGLSALSDSASGTLVHPATGRPVRADDPSFLAISNELADKGMLV
                     TTADALITWARTGSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIV
                     AGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYI
                     PGCPPSAEALLYGVLLLQKKIRRTGTIER"
     gene            complement(3449..3814)
                     /locus_tag="VGB93_03900"
     CDS             complement(3449..3814)
                     /locus_tag="VGB93_03900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0145294.1"
                     /translation="MPNLLAQYLPLVIFMVVAAIISAALAVAPFLLAFKAPDTEKLSA
                     YECGFNAFDDARMKFDVRFYLVSLLFIIFDLEVAFLFPWAVAFKAIGSAGFWAVMVFL
                     GVLTIGFVYEWRKGALEWD"
BASE COUNT          878 a         1207 c         1199 g          767 t
ORIGIN      
        1 cggacataaa tatagcagac atgcgcctgc atggcgaaag acgcgagcag acaaccttcg
       61 atcagaagat gtggatcgtt gcgcaggatc tcgcgatcct tgcaggtgcc gggttccgac
      121 tcgtcggcat tgacgacaag ataggagggc cgcgctgggt cgggcttcgg catgaaggac
      181 catttgagac cggtcgggaa gccggcgccc ccgcgcccgc gcaagcccga ggccttcatc
      241 tcattgatga cccagtcctt gcctttgtcg acgatcgtct ttgtattgtc ccagccgccg
      301 cgcgcctgcg cgcccttcag ccgccagtcg ccgaagccgt acagattgag gaagatgcgg
      361 tccttgtcgt cgagcatcat gggcctcaat tctcgttggc cggcgcggcg ctctgcacca
      421 cgggatcaga ctgcgggcca ctcaatccat cagggccgta aaggctcgtc agcgaggtca
      481 ggccgcctgc gggctctgag gtcttgcggc cagtttgcga gccaactttg accggacgcc
      541 cggctgcaag atcgtcgagc aatttgttga aattctcttc ggtcaggtct tcgtaataat
      601 cgtcgttgat ctgcaccatc ggcgcattgc agcaggcgcc gagacattcc acttcgagcc
      661 aagaaaacag gccatccgcc gtgacgtggc tttgcgcgcc gatgcgggcc tggagcactt
      721 tcttgatcgc atccgcgccg cgcaaaacgc agggggtcgt gccgcagaac tggatgaaat
      781 atttgccgac aggggcgaga ttgaacatcg tgtagaaggt cgcgacctca agcacgcgga
      841 tcgttgccat gccgagcttc gccgcgacat tctcgattgc cggcttcggc aaccagccgt
      901 cattctgctt ctgcgcgatc caaagcaggg cgatcacagc cgacgcttgg cgtccgggcg
      961 gatatttgga gatctcccgc ccgcagcgcg cctcattctc cggcgtgaat gcgaagcttt
     1021 ccggttgtat ttcggcgagg cggcgcacgc tcatcgatca acctccccga aaacaatatc
     1081 gagcgagccg aggatggcgc tgacatcggc gagcatcgtc ttgcggcaaa ggaagtccat
     1141 cgcctggaga tgcgcgaagc cgggcgcccg gattttgcag cgataaggct tattggtgcc
     1201 gtcggagacg agatagacgc cgaactcacc cttaggcgcc tcaacggcgg catagacttc
     1261 gccagcgggc acatggacgc cctcggtata aagcttgaag tgatggatca aagcttccat
     1321 cgaatgcttc atctcttcgc gcgacggcgg cgagaccttg cccttgacgt tgatcggccc
     1381 ctgcccgttc ggagacatga gcttctcgac acactgtttc atgatgctgg tcgactgccg
     1441 catttcttcc atgcggataa cctgacgatc gtaattgtcg ccatgcaagc cgaccgggat
     1501 gtcgaactcc atttcctcgt agcattcgta gggctgcgac ttgcgcaaat cccacgccgc
     1561 accggagccg cgcaccatga caccggaaaa gccccaacgc cagcaatctt caaggctgac
     1621 gacgccgata tcgacgttgc gttgtttgaa gatgcggttc tcgatgaaga gttcctgcag
     1681 atcgtcgagc actttaagga aaggatcgca ccaagcgccg atgtcttcga tcaggcgcgg
     1741 tggcagatcc tgatgcacgc cgccgggacg gaagtaagcc gcatgcatgc gcgagccgga
     1801 cgcgcgctca tagaaaacca tgagcttttc gcgctcctcg aagccccaaa gcggcggcgt
     1861 caaggcaccc acgtccatcg cctgtgtcgt gacgttgagg agatgcgaca ggatgcgacc
     1921 gatttcggcg tagagaacac ggatcaattg cgcccggcgt ggcacttcca caccaagcaa
     1981 tctctcaatc gcaagacaga aggcatgttc ctggttcatc ggcgcgacgt aatcgagccg
     2041 atcgaaataa ggcacgttct gcagataggt gcgcgcctcc atcagctttt ctgtgccgcg
     2101 atgcagcaga ccaatatgtg gatcgacgcg ctcgacgatc tcgccgtcga gttccagaac
     2161 aagacgcaac accccatgcg ccgccgggtg ttgcggaccg aaattgatcg agaaattgcg
     2221 gacgttctga tcggtcattg cggtttgact tgcgggtcct gaacgggcgg cacggtactg
     2281 gccttttcat cacccggaag gacgtaatcg accgcgccct cccatggcga gagaaagtcg
     2341 aagctacgaa actcctgccg caggttgacc ggctcgtaga caacccgctt ctgtgcatcg
     2401 tcgtaacgaa cctcgacata gccggtcatc gggaaatcct tgcgcaaagg atgtccctcg
     2461 aagccgtagt cggtgagaat gcgtcgcaaa tccgggtgac ccgaaaagaa aacgccgaaa
     2521 agatcgtagg cttcgcgctc gtaccaatcg gcggcgggat aaagcgccgt cagcgaggca
     2581 accggcacat cctcgtcggt tgcgaccttg atgcggatgc ggcggttgtg cttcggcgac
     2641 agcaaatgcg tcacgacatc gaagcgcggc acacgggtcg gataatcgac agccgtgaga
     2701 tcgataaagg aaatgaagag acaggacggg tcatcgcgca gataacgcac ggtctcgata
     2761 agccgcgccg gctcgacatc gagcgtcagt tcgtcatagg ctacgacgct ggcggcgatg
     2821 gcgccgggga gtgcagcagc gatcgaattt ccaagggcct gaagttcgtc gctcatccac
     2881 acaattcctc agcgctcgat cgtcccggtc cggcgtattt ttttctgcag cagcagcacg
     2941 ccgtacagca gggcctcggc tgagggcggg cagcccggta tgtaaatatc aaccggcacg
     3001 atccggtcgc agccacgcac caccgaataa gaataatggt aataaccgcc gccgttggcg
     3061 caggagccca tcgagatgac atagcgcggc tccggcatct ggtcgtagac tttgcgcagc
     3121 gcgggtgcca tcttgttggt gagcgtaccg gcgacgatca tcacatcgct ttgccgtggc
     3181 gatgcgcgcg gcgcgaagcc aaaccgctcg acatcgtagc gcggcatcga cacttgcatc
     3241 atctcgacgg cgcagcaggc gagaccgaat gtcatccaca tcagtgagcc ggtgcgcgcc
     3301 caggtgatca gggcgtcggc cgtcgtcaca agcatgccct tgtcggccag ctcgttgctg
     3361 atcgcaagaa aactcgggtc gtcggcgcgt accggcctgc ccgttgcggg atgaacgagt
     3421 gtgccggaag cgctgtccga gagggcgctc aatcccattc caacgctccc tttcgccact
     3481 cgtacacaaa gccgatcgtc aggacgccca ggaagaccat gacggcccag aaaccagcgc
     3541 tcccgatcgc cttgaaggcc acggcccagg gaaacagaaa ggcgacttca agatcgaaga
     3601 tgatgaacag cagcgagacg agataaaacc gcacgtcgaa cttcatgcgt gcgtcgtcga
     3661 aggcattgaa gccgcattcg taggccgaga gcttttcggt atccggcgcc ttgaaggcga
     3721 gcaaaaaggg cgccacggcc aaagccgcgc tgatgatcgc cgcgacgacc atgaagatga
     3781 cgagcggcag atactgtgcg agcaggtttg gcatggtcca tatatcctag gcggtagact
     3841 aggagacttc taattccaac cacgcgttcc ctaccgcagt gcaatcttcg gcgcaagcac
     3901 atgcccgtga gatttaccgc tcttgtcaaa cttttctttt cgttgaggta ggcagtgctc
     3961 aacgcgaata aattcaatac gttagagcca aatatgcatt atgcgccggt caggcccgcg
     4021 aacttcagtc gccgcgggtc gcggacgccg t
//
LOCUS       DASWOG010000190         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_205835,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000190 DASWOG010000000
VERSION     DASWOG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205835"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGB93_03905"
     CDS             <1..389
                     /locus_tag="VGB93_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145295.1"
                     /translation="DVVRKPIHVREILARVGAIGRRLEVEQDHANVGDMRVFFDGRDP
                     EIKGSILPLPRRERRILEYLVANRGRRVSKAQIFNSIYGIFDDNVEENVVESHISKLR
                     KKLRRHLGYDPINSVRYLGYRLAEAQ"
     gene            594..1826
                     /locus_tag="VGB93_03910"
     CDS             594..1826
                     /locus_tag="VGB93_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY0145296.1"
                     /translation="MSLFSALMASVSGMNAQANALSTISDNIANANTVGYKEASTQFE
                     DMLNEFSPSEYNAGGVGTVVNYSISAQGDPNATTDPTNMAIEGNGFFVVQNPSGQTFL
                     TRAGSFEPNSQGFLVNASGYTLMGYPPVPSPPSYSPNDLVPVQVSTSSLSPAVASTSA
                     TLTGNLDSSAATVAAADLPSATPPGSGFTSMTPVTTYDSLGNPTTLNVYMSNMGGGQW
                     EMDVYNSAGTPSGPPGAPLKTQDLQFSTTTGALQTPTTPVSVTVNGQAISFDPSKLTQ
                     LASSTSVQGAANGNTPGTYESVSVSQDGVLSETFSNGVTTPIYQIPLATVPAVNSMTS
                     MAGDVFEPNSDSGKIQLGGANNNGFGSINGSEEEASTVDLATELTNMVVTQNSYQANS
                     KAFSVGSDMLSELVNLLK"
     gene            1859..3325
                     /gene="flgK"
                     /locus_tag="VGB93_03915"
     CDS             1859..3325
                     /gene="flgK"
                     /locus_tag="VGB93_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY0145297.1"
                     /translation="MNLTTATNIAQQALSTVSAESATVSRNINGVNIPGFAQKLANVI
                     STYDGGSEVVSVTNAQNQALFGNVLGATSASATQAAISNGLTSLLSIVGEPGSDTSPA
                     EQLSDLTNAIQQYEASPSTSSLATAAVTAAEKLAGTLNSATATVQQVREQADTQMASS
                     VSSINSLLTQFQNVNQQIVTGTATGADVTDLQDTRNSILTQLSQQIGITTSTGANGEM
                     SVYTDSGVALDQGGTVSSVTFQPTQTYTAGTTGNAVYIDGIPVTGSSSTMPIQSGALA
                     GLATLRDDVTVTYQAQLDQTASGLISAFSETNPANSSDTEAGLFTNAGSTTLPTTAQT
                     TGLAGTISVNAAVDPSQSGNPELLGNGINFNFNTSDQGSFDTQLEQYLTNLSANQTFS
                     SAGQIGTTNTLEGYAAASVSWLDTEQQQVTSESAFQSTLLSTSTTALSNSTGINLDDE
                     MSQMLDLENSYSASAKLLTTINDMFADLANAVDQATSA"
     gene            3322..4365
                     /locus_tag="VGB93_03920"
     CDS             3322..4365
                     /locus_tag="VGB93_03920"
                     /inference="COORDINATES: protein motif:HMM:NF004669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated family protein"
                     /protein_id="HEY0145298.1"
                     /translation="MSSYISSYSISTALRQSILSEQTNLAQAQQEVSTGDYADVGLAL
                     GADAGQDIDLRNQESLLKTFTTTNNLASTNLSSIQNVLTELQTSAQSMVTNLTGATGQ
                     NGAGASLQEQAQSALQQLIAGLNTNSGGNYLFGGTNSSVAPITDYYASGSANQAAVNS
                     AFSSSFGFSQSDPGVANITASQMQSFLGGQFSSLFQGSSWTSDWSSASNTETTSEISP
                     SQTVDTSVSANNPAFQDIAQAYTMLASVGTQNLSSSTLQTVMTNAENLLNTGLTALTN
                     VQAGVGTAQSDITSANNQMSVEMNVLTTQIGNLEGVNPFDATTEVTNLQTQIETSYEL
                     TSQLSQLSLVKYL"
BASE COUNT         1013 a         1288 c         1227 g          927 t
ORIGIN      
        1 acgatgtcgt ccgcaagccg attcatgttc gtgagattct cgctcgtgta ggtgcgatcg
       61 gacgacgact cgaagtggag caggatcacg ctaacgtcgg cgatatgcgc gttttctttg
      121 acgggcgtga cccggagatc aagggcagta ttttgccgtt gccacgccgc gagcggcgca
      181 ttctcgaata tctagtcgcc aatcgcggtc gccgcgtgag caaggcgcag atcttcaatt
      241 cgatctacgg catttttgat gacaacgttg aagagaatgt tgtcgaaagt cacattagca
      301 agttgcgcaa gaagttgcgc cgccatcttg gctacgatcc gatcaattcg gtgcgctatc
      361 tgggctatcg cctcgctgag gcgcaatagt tggcgtgacg ctgcggcgtg ctccatgcgc
      421 cgaaatatga cgcctttaat ttcctgttaa atgacaagcg atgcgccggg agacgatcac
      481 gttttccggc gcatttgcgt ttgtttcgtt gtttcgcgtc gatgtgcgtt tcgcgcaagc
      541 cccgcacaag tcgctggcct tttaatcagc tcagacgagc aggagaaacg gcgatgagtc
      601 tttttagtgc attgatggcg agcgtgtcgg gcatgaacgc tcaggcgaat gcgctttcga
      661 ctatctcgga caacatcgcg aatgcgaata ctgtcggcta taaagaagcc tcgacgcagt
      721 tcgaggatat gctcaatgaa ttcagcccta gcgaatacaa cgccggcggc gtcggcacgg
      781 tcgtcaatta cagtatttcg gcgcaaggcg atccgaatgc gacgaccgac ccgaccaaca
      841 tggcgatcga gggcaatggt ttcttcgtcg ttcagaaccc gagcggtcag accttcctga
      901 cccgcgctgg atcatttgag ccaaactcgc agggcttcct ggtcaatgcg tcgggctaca
      961 ctttgatggg ctatccgccg gttccctcgc cgcccagcta ttcgccaaat gatcttgtgc
     1021 cggtgcaggt ttcgacatcg agcctttcgc ccgcggtggc gtcaacctct gcgacattga
     1081 cgggcaatct cgattcatcg gcggcgaccg tcgctgcggc tgatctgccg tccgctactc
     1141 cccccggttc gggcttcacc agcatgacgc cggtcacaac ctatgacagt ctcggcaacc
     1201 ccacgaccct gaatgtctac atgtcaaata tggggggcgg tcaatgggag atggacgtct
     1261 acaattccgc gggtacccca agcgggccgc cgggagcgcc gttgaagacg caggatctgc
     1321 aattcagcac gacgacgggc gcgttgcaga cgccgacaac gcccgtatcg gtcacggtca
     1381 atggtcaggc aatctcattc gacccgtcga agctgacgca actcgcttcc tcaacctccg
     1441 tccagggcgc cgccaatggc aatacccccg gaacttatga aagcgtctcg gtctctcagg
     1501 acggtgtttt gtccgagacc ttttccaacg gtgtgaccac gccgatctat caaattccgc
     1561 tcgcgacggt gccggctgtc aatagtatga cctctatggc gggcgacgtc tttgagccga
     1621 attcggactc tggcaagatt cagcttgggg gcgctaacaa caatggcttc gggtcgatca
     1681 atggatcgga agaagaggct tcgactgtcg accttgcgac cgaactgacc aacatggtcg
     1741 tgacgcagaa tagctaccag gccaattcga aagcattctc cgtcggctcg gacatgctga
     1801 gcgagctggt caatcttctg aaataatcgt cgttctcctc tgatcttgag ttcgtgccat
     1861 gaatctcacg actgccacga atatcgccca gcaggctttg tcgacggttt cagcggaatc
     1921 cgcgaccgtt tcacgcaata tcaatggcgt gaacattccc gggttcgcgc agaagcttgc
     1981 gaatgtcata tcgacctacg acgggggatc tgaggttgtc tcggtcacga acgcgcaaaa
     2041 tcaggcgctg ttcggcaacg ttcttggggc gacatctgcg tcggcaaccc aagcggcaat
     2101 ctcgaatggg ctcacctcgt tgttgtcaat tgtcggcgag cccggctccg atacgtcgcc
     2161 ggcggagcaa ctcagcgatc tcaccaacgc aatccagcaa tatgaggcgt cgccctcgac
     2221 tagcagcctc gcaacggccg cggtgactgc cgcagaaaag ctggcgggca cgctgaacag
     2281 cgcgacggcg acagtgcagc aggtccgtga acaagccgat acgcagatgg cttcgtcggt
     2341 ttcgtcgatc aattctctgt tgacgcaatt tcagaatgtc aatcagcaaa tcgtcaccgg
     2401 cacggccacc ggagcggatg tcaccgattt gcaggacacc cggaactcga ttctcacgca
     2461 actctcgcag caaatcggca tcacgacctc gaccggcgcc aacggcgaaa tgtcggttta
     2521 taccgacagc ggcgtcgcgc ttgaccaggg cggcaccgtg agcagtgtga cgttccagcc
     2581 gacgcaaacg tacacggcag gaacgactgg caatgctgtt tatatcgatg ggattccggt
     2641 gaccggaagc tcgtccacaa tgccgattca atccggcgcg ctggctggcc tcgcgacgct
     2701 tcgtgatgat gtcaccgtga cctatcaggc gcagctcgac cagacggcaa gcgggctcat
     2761 cagcgccttc agcgagacga acccggcaaa ttcaagcgat acggaggcgg ggctcttcac
     2821 caatgccggg agcacgacgc tcccgacaac tgcacaaacg acaggactcg ccggcacgat
     2881 ttcggtcaat gcggcggtcg atccgagcca gagtggcaat ccagagttgt tgggcaatgg
     2941 catcaatttc aatttcaaca cgtcggatca gggcagcttc gatacgcagc ttgagcaata
     3001 tctgaccaat ctttccgcca atcagacctt ctccagcgct ggtcagatcg gcacgaccaa
     3061 tacgcttgag ggatacgcag cggcttcggt aagttggctc gataccgaac agcagcaagt
     3121 cacgtccgag agcgcctttc aaagcacgtt gctctcgaca tcgacgacgg cgctctcgaa
     3181 ttcgacgggc atcaatctcg acgacgagat gtcgcaaatg ctcgaccttg aaaactccta
     3241 ttcggcgtca gcgaaattgc tgacgacgat taacgacatg ttcgccgatt tggcgaacgc
     3301 agttgaccag gcgaccagcg catgagctcg tacatctctt cttactcgat ttccactgcg
     3361 ctgcgtcagt ccattctctc ggagcagacc aacttggcac aagcgcaaca agaggtctcg
     3421 accggggatt acgcggatgt tggtctcgcc ctcggcgcgg acgcggggca ggacatcgat
     3481 ttgcgcaatc aggaatcgct gctgaagacg tttacgacga caaataatct ggcctcgaca
     3541 aatctgagca gtatacagaa cgtgcttact gagcttcaga caagcgccca aagtatggtg
     3601 acgaacctga ctggcgcaac cgggcagaac ggagccggtg cgagcttaca agaacaggcg
     3661 caaagtgcgc tgcagcaact tatcgcgggg ctcaacacga attcgggcgg aaattatctt
     3721 ttcggcggca cgaatagctc agtcgcaccg atcactgact attatgcttc tggttctgcg
     3781 aaccaagcgg cagtcaattc cgcattctcg tcttctttcg gcttttcgca aagcgatccc
     3841 ggcgtcgcaa atataacggc gagccagatg caaagcttcc tgggcggcca attctcatcg
     3901 ctcttccagg gttcgagctg gacatcagac tggtcgtcgg ccagcaatac tgaaacgaca
     3961 agcgaaatct cgccttcgca aaccgtcgac acctcagtca gcgccaacaa tccggccttc
     4021 caggatatcg cgcaggctta cacgatgctc gccagcgttg gtacgcagaa tttgagcagc
     4081 tcgacgttgc agactgtcat gacaaatgcc gaaaatcttt tgaacaccgg cctcacggct
     4141 ttgacgaatg tgcaggccgg cgtgggcacc gctcagagcg acattacgtc ggccaataat
     4201 cagatgtcgg tcgaaatgaa tgtcctgact acgcagatcg gcaatcttga gggagtcaat
     4261 cccttcgacg cgacgaccga agtgacaaat ctccagacgc agatcgaaac gtcctacgag
     4321 ctgacatcgc aactttcaca gctgagcttg gtgaaatacc tctaacccgc gttcttagga
     4381 agggcgatca gcaaccatgt atcagttctc ttaccaagag ataatggaag gcaacacgca
     4441 agatgcgcgc gcgcg
//
LOCUS       DASWOG010000191         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_150846,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000191 DASWOG010000000
VERSION     DASWOG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150846"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VGB93_03925"
     CDS             complement(<1..1014)
                     /locus_tag="VGB93_03925"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0145299.1"
                     /translation="MRIAVPAETDPAEARVAATPETVKKLIALGASVAIESGAGNKSS
                     FPDRDYAAAGANIVPDAVAALADADIILRVRRPEASHLGGAKKGALLLAILDPYGHID
                     ALQDIAATGVDAFAMELMPRITRAQVMDVLSSQANLAGYRAVVDAAAEYGRAVPMMMT
                     AAGTVPAAKAFIMGVGVAGLQAIATARRLGAIVTATDVRPATKEQVESLGAKFIAVED
                     EEFKQAETAGGYAKEMSAAYQEKQLALTASHIAKQDIVITTALIPGRPAPKLVTEAMI
                     ASMRPGSIIADLAAERGGNTALTRPGEKFTTDNGVTILGYLNVADRLAATASSLYARN
                     LLAL"
     gene            complement(1167..1313)
                     /locus_tag="VGB93_03930"
     CDS             complement(1167..1313)
                     /locus_tag="VGB93_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEY0145300.1"
                     /translation="MSSPIAADPNSEAEFAHHVQTYRLFINGFKYGAIGVIAILILLA
                     FLTL"
     gene            1521..2105
                     /locus_tag="VGB93_03935"
     CDS             1521..2105
                     /locus_tag="VGB93_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEY0145301.1"
                     /translation="MTLNPSLDGLQERVATALKRHTTYFLVEGIVLIILGFAAIAVPF
                     IATLTFTIFFGWLLLISGVVGLVSTFSLRPAAGFWWSLLSAILAIAVGFLLIARPGQG
                     AVSLTLLLIIFFIIEGFSSIIFATEYRQNLSGRWGWMVASGVVDLLLAGMIFLQLPSS
                     AIWAIGLLLGINMVFGGTTLVMLALDAKKVLPPT"
     gene            2124..>3092
                     /locus_tag="VGB93_03940"
     CDS             2124..>3092
                     /locus_tag="VGB93_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0145302.1"
                     /translation="MGLLNRARTWLSARLSERRAQLRLCLRVTVAAVLTFYISQLLHL
                     PWALWAVLTAVIMTQTSVGKSLIATIDYMIGTLGGGIYSGLVGIFVPHNSNLEIMVAL
                     GLALAPLAFLASLSPRFAAAPFTAVMVLLLPTITHASALQSAFFRVIEVAVGCAVSLA
                     VSFLVFPQRAHNLVYDGAAKILIRLADILPVLVADLSAPQADTPAHRLQRHVGDAFQK
                     LAAINIEGARERSAYLHSDPDPQPLIDVTLRLRHDIVLLGRAATEPLPPFILERLAQP
                     IEQFVVAVREFLISAAAALNARAEAAPIAQIDAAFANFSEAFAALRR"
BASE COUNT          535 a          980 c          927 g          650 t
ORIGIN      
        1 aagggccagg aggttgcgcg cgtagagaga cgatgcggtc gcagcgagac ggtcggcaac
       61 gttgagatag ccgaggatgg tcacgccatt atcggtcgtg aatttctcgc cgggccgcgt
      121 caaagcggta ttgccgccgc gctcggccgc aaggtcagcg atgattgagc ccggccgcat
      181 cgaggcgatc atcgcttcgg tgacgagttt cggcgccggg cggccgggaa tgagcgccgt
      241 ggtgatgacg atgtcctgct tggcgatatg cgaagccgtc agcgccagct gcttttcctg
      301 ataggccgcc gacatttcct tggcgtagcc acccgccgtc tcggcctgct tgaattcttc
      361 gtcctcgacc gcaatgaatt tcgcgccgag ggactcgacc tgttccttgg tcgcgggccg
      421 cacatcggtc gccgtgacaa tggcgccgag ccgacgcgcg gtcgcaatcg cctggaggcc
      481 ggcgacgccg acacccatga tgaaagcctt cgccgctggc acggtgcccg ccgcggtcat
      541 catcattggc accgcgcgtc cgtattcggc cgccgcatcg acgaccgcac gatagccggc
      601 gagattggcc tggctcgaca acacgtccat gacctgcgcg cgggtgatgc gcggcatcag
      661 ctccatggcg aacgcatcga cgcccgtcgc cgcgatgtcc tgcaaggcat cgatatggcc
      721 gtaaggatcg aggatggcga gaagcagcgc gcctttttta gctccgccga gatgcgacgc
      781 ctcggggcgc cggacgcgca gaatgatatc ggcgtcggca agggctgcga cggcatccgg
      841 cacaatgttc gcgcctgccg cggcataatc gcgatcggga aaagacgatt tgttgccggc
      901 gccggattcg atcgccacgc tcgcaccgag agcgatgagc tttttgacag tttccggagt
      961 cgcggcaacc ctggcttccg cagggtcggt ttcggcgggc acggctatac gcatgatgtg
     1021 cttccgatcg aacaatcaaa cgtcccgcat ccgcgggact tcgcgggacg acgaccgtgc
     1081 gaacccggaa aatccgaacg caggtcgaca caaatcgcca cggcgccccg cgcgcaaagc
     1141 ggcgcggcgc cacgagccag cgtgctttag agtgttaaaa aggcgaggag gatgaggatc
     1201 gctataacgc cgatcgcgcc atacttaaac ccgttgatga acaggcgata agtttgcaca
     1261 tggtgggcga attcagcttc cgaattcggg tcggcagcaa tcgggctgga catggaacct
     1321 ccctgttatc gacttttcac atcagtcatt cacttcgatc ggcgccgccg tcaaggggct
     1381 tcgcgccgag gggtgccgaa aatgatcaaa attggccgtt gcggtccaag gtttgacctt
     1441 ctgcaaaggt cagaagactt tatttgcggt agaatgaccc tgcttcgtcg ttgcttcgcg
     1501 ctcattccag gagacttttc atgaccctga acccatcgtt ggacggactg caggagaggg
     1561 tggccactgc cctcaagcgc catacgacct attttctggt cgagggcatc gtcctcatca
     1621 tcctcggctt cgcggcgatc gccgtgccgt tcatcgcgac actcacattc accatcttct
     1681 tcggctggct cctgctgatc agcggcgtgg tcggcctcgt ctccacgttc tccctgcgcc
     1741 ccgccgcggg cttctggtgg tcgctgctct cggcgatcct cgctatcgcg gtcgggttcc
     1801 tgctcatcgc ccggccaggc cagggcgcgg tctcgctcac cctgctgctc atcatctttt
     1861 ttatcatcga gggcttctcc tcgattatct tcgccaccga gtaccggcag aacctctccg
     1921 gccgctgggg ctggatggtc gcgagcggcg tcgtcgatct cctccttgcc gggatgattt
     1981 tcctgcaatt gccgagttcg gcaatttggg cgatcggcct gctgcttggc atcaacatgg
     2041 tctttggcgg cacgacattg gtcatgctcg ccctcgacgc aaagaaggtg ttgccgccca
     2101 cctaacggaa gaacgataaa aagatgggac ttttgaaccg cgcgcggacc tggttgtccg
     2161 cgcgcctcag cgagcggcgg gcgcaattgc gcttatgttt gcgcgtgacg gttgccgccg
     2221 ttctgacctt ttacatttcg caactgcttc atctgccctg ggcgctttgg gcggtgctga
     2281 ccgcggtgat catgacgcag acgagcgtcg gcaaatcgct gatcgcgaca atcgattaca
     2341 tgatcggtac gctcggcggc ggcatctaca gcggcctcgt cggcatattc gtcccgcata
     2401 atagcaatct cgaaatcatg gtcgcactgg gtctcgcgct cgccccactt gccttcctcg
     2461 cgagtttaag cccacgtttc gcggcggcgc ctttcaccgc ggtcatggtt ttacttctgc
     2521 cgacgatcac gcacgcaagc gcgctgcaat cggccttctt tcgcgtcatc gaagttgcag
     2581 tcgggtgcgc ggtatcgctt gccgtctcat ttctcgtttt tccgcagcgg gcgcacaacc
     2641 tcgtctacga tggcgcggcg aaaatcctga tccggttagc ggatattttg cccgtgctcg
     2701 tcgccgatct cagcgcgccg caagccgaca cgccggcgca tcggctgcaa cgccatgtcg
     2761 gtgacgcgtt tcaaaagctc gctgcgatca atattgaggg agcgcgcgaa cgctccgcct
     2821 atctccattc cgacccggat ccgcaaccgc tcatcgacgt aacgctacgg ctgcgtcacg
     2881 atattgtctt gctgggacgt gcggcgaccg agcctttgcc gccgttcatt cttgagcgtc
     2941 tcgcccagcc gatcgaacaa ttcgtggtcg ctgtgcgaga attcctgatc agcgcggccg
     3001 ccgccctcaa cgcgcgcgcc gaggccgcgc caatagcgca aatcgatgcg gcctttgcga
     3061 atttctccga agcattcgcc gccctgcgtc gc
//
LOCUS       DASWOG010000192         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_261121,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000192 DASWOG010000000
VERSION     DASWOG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261121"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VGB93_03945"
     CDS             <1..790
                     /locus_tag="VGB93_03945"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEY0145303.1"
                     /translation="YCRQQTANQGGGFLGGLFGGPSQNQGEPGIDTPPLPDAAAPPET
                     AQGPSGGSELLCVRHCDGAFFPIAYAPGRDQDTLADFCKASCPNTDASVYSRVPGAEI
                     QTAVGLDGKPYMSLPAALKYQKTLDPACSCRATGASWATTLADAEAMLSHSKKGEIVV
                     TQEKSDEMARPTAPGQAMSKKPRKPAADTDEMDDNGDDMTDRDAAAAAQVPTASTDSA
                     GISAGGVNNQTSYPQGAGETTEVTGADGVKRRVRIVGPQLSPAQ"
     gene            complement(741..>2030)
                     /locus_tag="VGB93_03950"
     CDS             complement(741..>2030)
                     /locus_tag="VGB93_03950"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0145304.1"
                     /translation="NARTALINFLFVTKDRGRFILRFDDTDLVRSREEYALSIENDLK
                     WLGIVPDAIVRQSQRFELYDAAAEKLRSVGRLYPCYETAEELERRRKRQQARGLPPIY
                     DRAALSLTAEDRVALEAQGRKPHWRFLLDHTIIRWNDLVRGDSHIDSASLSDPVLVRE
                     DETYLYTLPSFVDDLDLKISHIIRGEDHVTNTAVQIQLFEALGGAVPVFGHHNLLTTV
                     SGEGLSKRSGALSIGTLRDEGIESLAAAAFAVLTGTSDAVHPVQSLNELAADFDFSHV
                     SRNQAKFDPAELKSLSARTLHQMSFEAAEARLVAVGIEGPKAAYFWLAVRGNLEIFAD
                     VADWWGVVAGEIKPAIEDADFIAAARETLPAEPWDQATWSAWTNALKVRTNRKGRPLF
                     HPLRLALTGREQGPELAGLLPLIGRAKAEARLSGHAA"
BASE COUNT          414 a          627 c          637 g          352 t
ORIGIN      
        1 ctattgccgt cagcagacgg ccaatcaagg cgggggcttc ctcggcggtc ttttcggcgg
       61 cccaagccaa aatcaaggcg agccgggaat cgacacaccg cccttgcccg acgcggcagc
      121 gccgcccgaa acggcacaag gcccctcggg cggatcggaa ttgctctgcg tgcgccattg
      181 cgacggcgcg ttctttccga tcgcctatgc gcccggtcgc gaccaggaca cgctggcgga
      241 cttctgcaag gcctcctgcc ccaacaccga cgcgagcgtc tattcgcgcg tgccgggtgc
      301 ggagatccag acggccgtcg ggctcgacgg caagccctat atgagcctgc cggcggcact
      361 caaatatcag aaaacgctcg atccggcgtg ttcgtgccgg gcgaccggcg cttcatgggc
      421 aacgacgctt gccgacgcgg aagccatgct gagtcacagc aaaaagggcg agatcgtcgt
      481 gacgcaggag aagtccgacg aaatggcgcg cccaacggcg cctggtcaag cgatgagcaa
      541 aaaaccacgt aagccggcgg cggacacgga tgaaatggac gacaatggtg acgacatgac
      601 cgaccgggat gcggcggccg cggcgcaagt gccgacagcg agcaccgatt cggcgggcat
      661 ttccgcaggc ggcgtcaaca atcagacgtc ctatccgcaa ggtgccggcg agacgaccga
      721 ggtcaccggc gccgatgggg tcaagcggcg tgtccggata gtcgggcctc agctttcgcc
      781 cgcccaataa gcggtaggag cccggcgagt tccggcccct gctctcgccc ggtcagagcc
      841 agcctcagcg gatgaaacaa aggccggccc ttgcggttcg tgcggacctt cagcgcattt
      901 gtccaggctg accaggttgc ctgatcccaa ggttcggcag gcaaggtttc gcgcgccgcg
      961 gcgataaaat ccgcgtcttc aatggccggt ttgatttccc ccgcgacgac gccccaccag
     1021 tcggcgacat ctgcgaatat ttcgaggttg ccgcgcactg ccagccaaaa atacgccgcc
     1081 ttcggccctt ctatgccgac ggcgacgagt cgcgcctcgg cagcctcgaa gctcatctga
     1141 tgaagggtgc gcgccgagag gcttttgagc tcggccggat caaacttcgc ctgattgcgg
     1201 ctgacatgcg aaaaatcgaa atccgcggcc agctcattga gcgattggac gggatgaacg
     1261 gcgtccgacg ttcctgtcag aacggcgaag gcggcagccg ccagggattc aatcccctcg
     1321 tcccgcaacg tgccgatcga gagcgcgccg ctgcgcttcg ataatccctc gcccgacacc
     1381 gtggtcaaca gattatggtg gccgaacacc ggtacggcgc cccccaaagc ttcgaaaagc
     1441 tgaatctgga ccgcggtatt ggtgacgtga tcttcgccgc ggataatgtg gctgatcttg
     1501 agatcgaggt cgtcgacgaa tgagggcagc gtgtagaggt aggtttcatc ctcgcgcacg
     1561 agaaccggat cggagagcga ggctgaatcg atgtggctgt cgccgcggac aagatcgttc
     1621 cagcggatga tggtgtgatc gagcagaaaa cgccaatgcg gctttcgccc ctgcgcttcg
     1681 agtgccaccc gatcttcggc tgtcaatgaa agtgcggcgc ggtcataaat ggggggcagc
     1741 ccacgcgcct gctggcgttt gcggcggcgc tcaagctcct cggccgtttc atagcaggga
     1801 tagagtcgcc cgaccgaccg caatttctcc gcagctgcat cgtaaagttc gaatcgctgc
     1861 gattgacgga caatggcgtc cggcacgatc ccgagccatt tcaggtcgtt ttcgatggat
     1921 aacgcgtact cttcccgtga ccggaccagg tcagtgtcat cgaaacgcaa aatgaatcgg
     1981 ccacggtctt ttgttacaaa aagaaaattg ataagcgccg tccgggcgtt
//
LOCUS       DASWOG010000193         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_352545,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000193 DASWOG010000000
VERSION     DASWOG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352545"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..678)
                     /locus_tag="VGB93_03955"
     CDS             complement(61..678)
                     /locus_tag="VGB93_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145305.1"
                     /translation="MKILVIDDHGIVREGVRRLLGTIPGSEVVEAGTAQQGLSKLRSE
                     SPDIIVLDINLNGTSGLELLQRLKNENKATRIVMFTMYSEAGYVSRAIRGGASGYVSK
                     SAPADELVTAVKRVAAGQRYVDREAANELVFSASNEEDPLQKLSNREMEILRLLGEGK
                     SLLEIANTFGIAYKTVANSCSRLKEKLGLERTTDLIRFSLQNRQA"
     gene            complement(675..2033)
                     /locus_tag="VGB93_03960"
     CDS             complement(675..2033)
                     /locus_tag="VGB93_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0145306.1"
                     /translation="MPLRLLLVRSIALIFLATLALSTLLVFWRAYEKVEADMTASLAA
                     AQRAVANVADETDGITQPGWRLQHIVANFNGDRNVRATLQTADNSLVHSILAPPARPV
                     PSFLYELLAGTPLVAIVDLPSALKSSGTLRLETDAHNDILGLWDDAKNLLFLLALFAA
                     TCLLVLYFMLGRALRPLEELTRGFARIGNGDYRARVPVFAPRELAKLSAGFNDMAVRL
                     SEMEHRNHRLHEQLETVQEEERIELARNLHDDISPLLFSADVDAMTIRELAHTKDFPA
                     IVDRAHAIRGAVAEMKRNVKAILGQLRPSGLHALGLASAVDNLMSYWKSRRPEIAFSV
                     KAPDKTWGPRVDGALYSIIRESLNNAVKHSRPSRIEVLIEETYDGGFLVARVSDNGGG
                     FDANNASGGFGLIGMKERATLLGGTLTVENRSDRLGVVVTTRLPLPSDEDLEFAQGMA
                     AQ"
     gene            complement(2161..3291)
                     /locus_tag="VGB93_03965"
     CDS             complement(2161..3291)
                     /locus_tag="VGB93_03965"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0145307.1"
                     /translation="MAQSWSSSKSVQIDQIIAHFRELNGTDEATLPSISVSIGMDGRL
                     VAAKGYGASDGKPVDGHTLYEIGSITKQFTAAVTLEMIKGGAISTRSGKKLDLATPLV
                     AVFDDSSFWKAQPWLTVGRLLTMQSNLPNFTHRPPTGTNPWQPISADTLFQDIESQPP
                     SSVSDDFDYSNTNYFLIAELMEQCRLPGEAQPETYHELLRKRIFEPAGMVETRFMDDA
                     ETNFEADAAAPAAPVSTGLKEQAATWARPDYGRRRRPAFTNPDWLKGSADAVSSAVDL
                     FAWDRAMMDPKIVPSDIRDTMLSGQARVSPTMYYGMGWFYEERDDAAVYSHSGAVPGY
                     TSFNEIVRQKDGRWFSITILSNSDQLDGLDDLANSIAYILAM"
BASE COUNT          732 a         1120 c         1203 g          768 t
ORIGIN      
        1 cagggcgccg gcgcatgcga aggcgcagat gaattcgcag caccactggc gctgaaatcg
       61 ctaagcttgg cggttttgca gcgaaaagcg gatgaggtcc gttgtccgtt caagcccgag
      121 cttttccttg aggcgtgagc aggaattcgc cacggtcttg taggcaatgc cgaaggtatt
      181 ggcgatttcc agcaagcttt tgccttcgcc cagcaaccgc aaaatttcca tttcgcggtt
      241 gctcagcttt tgcagcgggt cttcctcgtt ggaagctgag aagacgagtt cgtttgccgc
      301 ttcgcgatcg acataacgct ggccggcggc gacgcgtttg accgccgtga ccaattcgtc
      361 ggccggcgcg ctcttgctca cgtatcctga agcaccgccg cgaatggcgc gcgagacata
      421 gccggcttcc gaatacattg tgaacatgac gatacgcgtc gccttgttct cgttcttcag
      481 ccgttgcagc aattcgagcc cgctggtgcc gttgagattg atatcgagga cgatgatatc
      541 gggcgattcg ctgcgcagtt tcgacaggcc ttgctgcgcg gtgccggcct cgacgacttc
      601 ggaaccgggg atggtgccga gcaggcgccg cacgccctcc cggacgatgc catgatcgtc
      661 gatcaccagg atcttcattg cgccgccatc ccttgcgcga attcgaggtc ctcgtcgctc
      721 ggcaagggca agcgcgtggt gacgacgacg cccaaccggt cgctccggtt ctcgactgtc
      781 aaagtgccgc cgagcagcgt cgcgcgttcc ttcattccga tcaagccgaa gcctccggag
      841 gcgttgttgg cgtcgaagcc gccgccgttg tccgacacgc gcgcgacaag aaaaccgccg
      901 tcgtatgttt cctcgattaa aacttcgatg cgtgatggcc gcgaatgctt gaccgcgttg
      961 ttcaggcttt cgcggatgat gctgtaaagg gcgccatcga cacgcggtcc ccaggtcttg
     1021 tccggcgcct tgacgctaaa ggcgatctcg ggacgtctcg acttccaata ggacatgagg
     1081 ttatcgacgg cgctcgccaa gcccagcgca tgcaggccgg atggccgcaa ctggccgagg
     1141 atggccttga cgttgcgctt catctcggcg acggcgccgc gaatagcgtg ggcgcggtca
     1201 acaatggccg gaaagtcctt ggtgtgcgcc agttcgcgga tggtcatcgc atcgacgtcg
     1261 gccgaaaaca gaagcgggct gatgtcgtca tgcaggttgc gggcgagttc gatgcgctct
     1321 tcttcctgca ccgtctcgag ctgttcgtgc agccgatgat tgcgatgctc catttccgaa
     1381 agacgaaccg ccatgtcgtt gaagccggcg ctcagctttg caagctcgcg cggtgcgaag
     1441 acggggacgc gggcgcgata gtcgccattg ccgatacgcg caaacccgcg ggtcagttcc
     1501 tccagtggcc ggagcgcgcg gccgagcatg aaatagagca cgaggagaca cgttgccgca
     1561 aagagcgcga gcagaaatag caaattcttc gcgtcatccc agaggccgag gatgtcgtta
     1621 tgcgcatcgg tctcaagtcg caacgtgccg ctgctcttca aagccgaagg cagatcgact
     1681 atcgctacga gcggcgttcc cgcgagaagt tcgtagagaa atgacggcac cggacgcgcg
     1741 ggcggcgcga ggatggaatg caccagactg ttgtccgctg tttgcagcgt ggcgcggacg
     1801 tttctgtcgc cgttgaaatt ggctacgata tgttggagac gccagcccgg ttgggtgatg
     1861 ccgtcggttt cgtcggcgac gttggcgacg gcccgctgcg cggcggcgag cgaggcggtc
     1921 atgtcggctt cgactttttc ataggcccgc cagaagacca gcagggtgct cagcgccagc
     1981 gttgcgagga aaataagcgc aatcgaacga acgagcagaa ggcgcaacgg caacgacgaa
     2041 tcctcacaag cgacccgcga tttccggaaa tttcccgccg ggggcgacat ttatctgtca
     2101 ccttatagca ggttcgccgg gtgctggtct ccccctattc gggaagacgc ggcagccggc
     2161 tcacatggca aggatgtagg cgatactgtt ggcaaggtcg tcgagcccat caagctggtc
     2221 gctattgctc aggatggtga ttgaaaacca gcgcccgtcc ttctggcgga cgatttcgtt
     2281 aaaagacgtg tagccgggga ccgcgccgga atgggagtag acagccgcgt cgtccctctc
     2341 ctcgtagaac cagcccatgc cgtaatacat cgtcggcgaa acccgggcct gcccgctgag
     2401 catcgtgtcg cggatgtcgg acggcacgat cttggggtcc atcatggccc tgtcccaggc
     2461 gaagagatcc acggcgctgc tcaccgcgtc ggcgctgccc ttgagccaat cgggattggt
     2521 gaaggccggt cggcgccggc gtccgtagtc ggggcgcgcc caggtggctg cctgttcctt
     2581 tagccccgtg gagactggcg cggcgggggc ggcggcatca gcctcaaaat tggtttcggc
     2641 atcgtccatg aagcgcgtct cgaccatgcc cgcgggctcg aaaatccgct tgcgcaggag
     2701 ctcgtgatag gtctccggct gtgcctcgcc gggcagacgg cattgctcca tcagttctgc
     2761 gatgagaaag taattcgtat tgctgtaatc gaaatcgtcc gagacgctcg aagggggctg
     2821 gctctcgata tcctgaaaca gcgtatcggc gctgatcggc tgccaggggt tcgtcccggt
     2881 cggtggccgg tgcgtgaagt ttggcaggtt ggactgcatg gtcagaagcc gcccgactgt
     2941 cagccagggc tgcgctttcc agaaagaact atcgtcgaag acggccacaa gtggcgttgc
     3001 cagatcgagc tttttgccgg agcgtgtcga gatggcgccg cctttgatca tctcgagcgt
     3061 aacggcggca gtaaattgtt tagtgatcga gccgatctca taaagcgtat ggccgtcgac
     3121 gggcttgccg tcgctggcgc catagccttt cgccgccacg aggcgcccgt ccatgccgat
     3181 tgagacgctt atgctgggca gcgtggcttc gtcggtgccg ttcaactcac gaaaatgcgc
     3241 gatgatctgg tcgatctgga cggatttact cgacgaccag gactgcgcca acgaagggga
     3301 ggcgacgaga aaagggaggc aaatggcaac tcccatcgcc gcccgcgctc tccgccgcag
     3361 accgtggata tttcgaatcg agttggtcca tagattcatg cggacttcgc cattgcgccg
     3421 ccgcctcgca ttcagaccgc gcggtaaacc agcgcaggtc gaacaaaatg ggcgaagcga
     3481 tccgccggat caagaagcag ttttcaggcc ggatgaggtt ttgcccagca gatcgctgga
     3541 gatctgtccg tcagtggtcc gagccggcct caccgaagct cagataattt cccgaaaaac
     3601 accgagtaaa gcgcccgatt tatggctcaa cgagtctcgt tccggcagcc taaatcggaa
     3661 ctttccttcc cgatggccgg cgctaaggtc agcactgctg caccttttca aggcggtgag
     3721 cgacagacgc cgccttcgaa tgtccggatt gtttcgatcc ggacccccca aagccttgag
     3781 cgtttcccgc gtgcgggaaa cgctcatttt tatatggcgc gga
//
LOCUS       DASWOG010000194         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_188028,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000194 DASWOG010000000
VERSION     DASWOG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188028"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VGB93_03970"
     CDS             complement(<1..932)
                     /locus_tag="VGB93_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0145308.1"
                     /translation="MHRLATPVTGGAGAILMFHRVRPSQPRDFAPNLLLEITPDFLDA
                     VITRVRALGFTVVDIDTALTMIAESSGQKFAVLTFDDGYKDTRDIALPILEKHAAPFT
                     MYVTKGFAERTARLWWVELEDAIARLSQVQIAIGERLFDLPAANAAEKNAAFATLYQA
                     LVDGSEEDLLAVIADLARQAGIDPLSIPEELCLDWAGIETLAKHELCTIGVHTLTHPR
                     LGKLTDADMRRELADSRAQIEAHIGKEAAHLAYPVGNPVAAGAREFQAAAELGFKSAV
                     TTRPGMIFPAHDDRRTALPRLSINGTWQSLDYVE"
     gene            1088..2359
                     /locus_tag="VGB93_03975"
     CDS             1088..2359
                     /locus_tag="VGB93_03975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145309.1"
                     /translation="MSSVIEFYPSTAARRLRLVEGVPGRAFAKVEVHRTPDAVEADWA
                     ELEVLATASIYQTRAFVVPWLETLGAARKITPLFVTAKDSFGRTIALLCLGVRSYGWF
                     RVASFLGGRESNFNFGLFRPGSHFSPADLRALLREAAKAAGKHAPDVFVLKNQPYEWN
                     AAQNPFAFLPHRPSPSAAYATELGSDAAAFLKSKLSKDTQKKLRKKEARLAEIGPVEL
                     LTGEDPENARLILDAFFFEKIRRCEEKAITSDFAAPSMRSFFERLCRHGNTSANRWLE
                     LYGLALGGRIIATYIGAAHRGHFSAMVNSFDSDPEIAKSSPGDLLLMKLVAAQCTDDR
                     TSFDLGIGEARYKMTYCDIKVPLFDVVVPLGARGYAVAARQAICSRLKTAIKERPQVF
                     AALRQAKYFVAHKRFAGAPSLSPKPSPELSH"
     gene            3003..3554
                     /locus_tag="VGB93_03980"
     CDS             3003..3554
                     /locus_tag="VGB93_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145310.1"
                     /translation="MLHHVWRPAAALAGLAFGAGLAFGGTAQAESVQKECSVKYQAAK
                     SSNSLNGETWNQFYKQCAAELKGGGSAAAPASAPASAPPPATTTTQAAPPPKKHHWWA
                     GGGNNSQAGTPSAPPPMSTGNVVFPNAVSSQFSNLKPGRARMETCVAQYRANKANGGN
                     GGLKWIEKGGGYWSECNKHLKGI"
     gene            complement(3613..>4274)
                     /locus_tag="VGB93_03985"
     CDS             complement(3613..>4274)
                     /locus_tag="VGB93_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0145311.1"
                     /translation="LVKLPKSISYETGAAMLLKGLTAQYLLRQTHEVKKGETILVHAA
                     AGRMGLILCQWGKALGAHVIGTAGSPEKAKLAKKAGAKQVILYRAENFVEAVKKITKG
                     KLCDVVYDGIGKDTYPGSLDCLRPLGLLASYGASSGQIENFNLGLLAQKGSLYVTRPT
                     LNTYAGADEDLQKMARDLIRVISDGLVKIPYTTHPLSEIVAVHEALESRATTGSTVLI
                     P"
BASE COUNT          894 a         1278 c         1232 g          870 t
ORIGIN      
        1 atctcgacgt aatcgagcga ttgccaggtg ccgttgatcg agagtcgcgg cagcgccgtg
       61 cggcgatcat catgtgcagg gaaaatcata ccaggccgcg tcgtcactgc gcttttgaag
      121 ccaagctcag cggcagcctg aaactcacgc gccccggccg cgacgggatt gccgacggga
      181 taggcgagat gcgccgcctc cttgccgata tgcgcctcga tctgcgcgcg gctgtcggca
      241 agctcgcggc gcatgtccgc gtccgtcaat ttgccgagcc gcggatgcgt gagcgtgtga
      301 acgccaatcg tacaaagctc gtgtttggcg agcgtttcga tccccgccca atcaagacaa
      361 agctcttccg ggatcgaaag tggatcgata ccagcctgtc gcgcgagatc ggcgatcacg
      421 gcaagcaagt cttcttccga tccgtcgacc aacgcctggt agagcgtcgc aaatgccgcg
      481 ttcttttcgg ctgcatttgc cgccggaagg tcgaagaggc gctcgccgat cgcaatctga
      541 acttgcgaca gacgtgcgat ggcatcttca agctcgaccc accagaggcg cgcggtgcgc
      601 tcggcaaagc ctttcgtgac atacatggtg aagggcgcgg catgcttttc aaggatcggc
      661 aaggcaatgt cgcgcgtatc cttgtaacca tcgtcgaacg taaggaccgc aaacttctgt
      721 ccagaacttt ctgcaatcat ggttaacgcc gtatcgatgt cgacgaccgt gaagccgaga
      781 gccctcacac gggtgatgac agcgtcgaga aaatcgggtg ttatttcaag gagaaggtta
      841 ggagcgaaat ctcgcggctg cgaagggcgc acgcgatgaa acatgaggat tgcaccggca
      901 ccgccggtga ccggggtcgc gagccgatgc aacccggtcc gtcggaaaaa gctaaagcct
      961 gaagatatta tttgcctttt cgagatgaac acatgaacct ccgcaaaaca ctatatatta
     1021 tggcaagatg tcgttgacct ttggttgcta acgttttcgc ttctgctcgt gggtcgggac
     1081 agcgtaattg tcgagcgtaa tagagttcta tccatccacc gccgcgcggc gccttcgcct
     1141 tgtcgaaggt gtgcccggcc gggctttcgc caaggtcgaa gtgcatcgca cgcctgatgc
     1201 cgtcgaggcg gattgggccg agctcgaagt gctggccacg gcttcgatct atcagacccg
     1261 cgcgttcgtc gtgccatggc tcgaaacttt gggtgccgcg cgaaaaataa cgccgctctt
     1321 cgtcaccgcc aaagactcgt tcggccgtac aatcgcccta ctctgcctcg gcgtgcggtc
     1381 atatggctgg tttcgcgttg caagctttct cggcggacgt gaatccaatt tcaatttcgg
     1441 cttgttccgc cccggctcgc atttcagccc tgccgatttg cgcgcgcttt tgcgcgaggc
     1501 ggccaaggcg gcgggcaaac atgcgcccga cgtcttcgtt ctcaagaacc agccttacga
     1561 atggaacgcg gcgcaaaacc cttttgcgtt tctgccacac cgtccgagcc ccagcgctgc
     1621 ttatgcgact gagctaggct ccgacgcggc tgcgttcctg aagtcgaagc tctcgaaaga
     1681 tacacaaaag aagctgcgca agaaagaagc acggctcgcg gaaattggcc cggttgaact
     1741 gctcaccggc gaggatcccg agaacgcgcg gctgatcctc gatgcatttt tctttgagaa
     1801 aattcgccgc tgcgaagaaa aagcgatcac atctgatttc gccgcgccat cgatgcgcag
     1861 ctttttcgag cgcctctgcc gtcacggaaa cacttcggca aaccgctggc tcgaactcta
     1921 cggtttggcg ctcggcggcc gtatcatcgc gacctacatt ggcgcagcgc atcgcggcca
     1981 cttcagcgcc atggtcaatt cattcgacag cgatcccgag atcgccaagt cgagtcccgg
     2041 cgatctgctt ctgatgaagc tcgtcgctgc gcaatgcacg gatgatcgta ccagcttcga
     2101 tctcggcatt ggcgaagcac gttacaagat gacctattgc gatatcaaag ttccgctgtt
     2161 cgatgtggtc gtgccgctcg gtgccagggg ctacgcggtc gccgcgcgcc aggcgatctg
     2221 ctcgcgtctc aagaccgcga tcaaggaacg cccgcaagtc ttcgccgcgc tgcgtcaggc
     2281 taaatatttt gtcgcccata aacgtttcgc aggtgccccc tcgctcagcc cgaagcccag
     2341 ccccgaactg tcgcattaac ttctaagaat tacgacaaac gtcgaagcaa cgacgcggcc
     2401 cggcgcgcat cgcacaatag gcgtgcgaac ttgaccttcc ccgaacgcca ggatccggtg
     2461 cgcttttcgt cgatcaactc tgtgccaagg ttcagtcgtt gggagacgtt ggccctgatc
     2521 gccggccccg ccgcaaagta aaagggccaa atgcgtgcgt cccaacacaa gtcgttcgaa
     2581 ggttcgattg accgaaagct tccccggcgc atagattttc ggcgcgttca cctgaatcgc
     2641 cccccgagga ggcaaaatga cgaaagtatt ttcactcgtt gtggcactgt cgttttgtgc
     2701 cagcgccgca atggcggaaa cttgtcattc ccgcgcgacc gataagaatt tgcacggcgc
     2761 cgctctcaca agcttcatga agaagtgcgt gcgtgaaacg ggtggcacat gcagcgccat
     2821 ggccgcagac aagaagctgc acggcgctgc caagacaagc ttcgagaata aatgtcttcg
     2881 cgataacatt ggaaactaag tttcgcaggc attcaggggc agttcatcgg cgcaatctac
     2941 gttgcgccaa agtcattttc cgcggcgcgg cggatttgcg cgctcatcat gaggaggaat
     3001 ttatgttgca tcatgtttgg cgcccggccg ccgctttggc tggcctcgcc tttggagccg
     3061 gacttgcctt tggcgggacc gcgcaagcgg aatcggtcca gaaggaatgt agcgtcaagt
     3121 atcaggcggc aaagtcgtcc aattcgctca atggcgaaac ctggaaccag ttctacaagc
     3181 agtgcgcggc cgaactgaaa ggcggcggaa gcgccgcagc gcccgcttct gctccggctt
     3241 cggcaccccc gccggccaca acgacgacac aagccgcacc gccgccgaag aagcatcatt
     3301 ggtgggcagg cggcggcaac aacagccagg ccgggacgcc atcggcgcct ccgccgatga
     3361 gcacgggtaa tgttgtcttc ccaaatgctg tctcctcgca attttctaac ctaaagcccg
     3421 gccgcgcgcg catggagacc tgtgtcgcgc aatatcgcgc caataaggcg aacggcggca
     3481 acggcggcct gaaatggatt gagaagggcg gcggctattg gagcgaatgc aacaagcact
     3541 tgaagggcat ctaagcagcc gcttcttcgc aaggaaaaag aaatggccgg gtttttcccg
     3601 gtcattttta ttttagggaa tgagcaccgt cgaacctgtc gtcgcgcgcg actcaagcgc
     3661 ctcatgcacg gcaacgatct cggacagcgg atgcgtcgtg tatgggattt tcacgagtcc
     3721 atcggagatg acacggatca gatcgcgcgc catcttctgc aagtcctcgt ccgcacctgc
     3781 gtaggtgttg agcgtcgggc gcgtgacata gagcgagcct ttctgtgcca ggaggccgag
     3841 attgaaattc tcgatctggc ctgacgaagc gccatagctc gcaagcaacc ctaagggccg
     3901 cagacaatcg agcgagcccg gataggtatc cttcccgata ccgtcgtaaa cgacgtcgca
     3961 gagcttgccc ttggtgatct tcttcaccgc ttcgacgaaa ttctccgcgc gatagaggat
     4021 gacctgtttg gcgcccgcct tcttggcgag cttcgccttc tcgggcgatc ccgccgtgcc
     4081 aatgacatgc gcgccgaggg ccttgcccca ttggcaaagg atgagcccca tacggcccgc
     4141 tgccgcgtgg acaaggattg tttcaccctt tttgacctcg tgcgtctgcc gcagcagata
     4201 ttgcgccgtc agccctttca acagcatcgc ggcgccggtc tcgtaggaaa tcgacttcgg
     4261 caatttgacg agac
//
LOCUS       DASWOG010000195         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_77639,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000195 DASWOG010000000
VERSION     DASWOG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77639"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1005)
                     /locus_tag="VGB93_03990"
                     /pseudo
     CDS             complement(244..1005)
                     /locus_tag="VGB93_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(1028..>1689)
                     /locus_tag="VGB93_03995"
     CDS             complement(1028..>1689)
                     /locus_tag="VGB93_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0145312.1"
                     /translation="VRGRSVYDVRKAMGKQLARETAIDADVVVPVPDSGVPAALGFSQ
                     ESGIPFELGIIRNHYIGRTFIQPTQSIRELGVRLKHSANRAAIAGKRIILIDDSIVRG
                     TTSVKIVQMMREAGAREVHFRISSPPITHPDYYGIDTPERDKLLAATHSLDEMRDYIG
                     CDSLAFLSVEGLYRAMGEPARDPVRPQFTDHCFTGDYPTNLTDVAGETKSQLSLLAEV
                     G"
BASE COUNT          344 a          489 c          549 g          307 t
ORIGIN      
        1 tcgaatatac gccgacctcc ggaaaggagg cgaagcgtcc ggcattacgc aatgcggcgc
       61 agttgcagca gttgcaagac gagctcgaag acggcggtgc gcataatgaa cccgtcacga
      121 aggcgaaggt ccggcatttt caggccgtgc cgaaaaaggt cgtggcgaag aaagtcgcga
      181 caaagaagct aaagaaaaag acgctcgcat cgtcaccaat gcgtattcca ccgacctcga
      241 ttgcggaccc gcgaagctga gaatttcatc gctcgggaag tcgtacagtt tgccggtttg
      301 taccgatgac ggcagcagga attgcaaaag ctttggcgcc agatcctcgg gcgtcttgag
      361 cgtcaacggg tcttcgcctg gcatggcctt ggcacgcatc cgcgtacgta gcggccccgg
      421 attggccagc atgactttta cgttagaggt cgtcgccgtt tccgccgcgt aggtacgcgc
      481 caaagcatcg agcgccgctt tggaaacggc atagccgccc caatagggtc gatagtcggc
      541 acggtgcgca ataccggacg tgatgaacag aacccgtcct gcctcggccg cccgtagcgg
      601 catatcgagc gcgtggatca gccgccagtt cgcggtgaca ttgacggcga cgactttgtc
      661 ccagtctttc ggatcgatat gcgagagcgg cgtaatggcg ccgagttcgc ccgcattgcc
      721 gacgaagata tcgagctttt tccatcgttc gtgaatggcc gcgccaagcc ggtcgagcgc
      781 gtcgaaatcc gtcaggtcgc acgggacgag cgtcgcctcg ctgcctgcgg cgcgaatcgc
      841 atcgtcgagg tcttccagag cgccctgggt gcgcgcaagg gcgatgacat gcgcgccgcc
      901 gcaggcgagt tccagcgcga gcgcatggcc aatgccgcgg gacgcaccgg tgacgagcgc
      961 cacgcggccg gagaatggct gcgcgggaga actctgctca gccatgttgg atctctagag
     1021 attgcggcta gccgacttcg gcgaggagcg agagctgcga cttggtctcg ccagcgacgt
     1081 cggtgagatt ggtgggataa tcgccggtga agcaatgatc ggtgaattgt ggccgcacgg
     1141 gatcgcgcgc cggttcgccc attgcccgat aaagcccctc gaccgacagg aaggcgagcg
     1201 aatcgcagcc gatataatcg cgcatctcgt cgagcgaatg cgtcgcggcg agcagcttgt
     1261 cacgctcggg cgtatcaatg ccgtaataat ccggatgcgt gatggggggc gaggagatgc
     1321 ggaagtgaac ctcgcgcgcg cctgcctcgc gcatcatctg cacgattttt accgaagtcg
     1381 tgccacgcac aattgaatca tcgatgagga tgatgcgctt gccggcgatc gcggcgcggt
     1441 tggcgctgtg tttcaagcgc accccgagtt cgcggatcga ttgcgtcggc tggatgaagg
     1501 tgcggccgat ataatggtta cggatgatgc cgagttcgaa cgggatgccg gattcttgcg
     1561 agaagccgag tgccgcggga accccggaat ccggcaccgg cacaacgaca tccgcgtcta
     1621 tcgccgtttc tcgcgcaagc tgtttgccca tcgccttgcg cacatcataa accgagcggc
     1681 cgcggacga
//
LOCUS       DASWOG010000196         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_279427,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000196 DASWOG010000000
VERSION     DASWOG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279427"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1565)
                     /locus_tag="VGB93_04000"
     CDS             complement(483..1565)
                     /locus_tag="VGB93_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145313.1"
                     /translation="MDSSADPQAFDDKKSESVPEGAMDAASAAPDEANSMSHALTKFE
                     PKAATSASEAESREPPEVESFPGSALVILPPTKEKIGGGFSAGERLGGPARTKRSSLT
                     NNAMRAAAVLLIAGGAYAVVSHYLSAPSASVPIQVAAMTPQTAAMAAVAPPAAGPASL
                     AASASADEPQLAALRHETVALNNEVHKLLARLAALQGEPDQMRSLKKSLDGLKAGFEA
                     EKADYKAQIAQLTSKLDHTHAATAEKARVARLDAQAIQATLDRAARTETASGDSTGSI
                     PNAAAPGPVTLASAEAPRHASQPLNDWVVRDVYRGIALVEGPQGAVEVMPGDTLPGAG
                     TVRAIERRGDGWVVVTSRGYVDYEHD"
     gene            1780..>2393
                     /locus_tag="VGB93_04005"
     CDS             1780..>2393
                     /locus_tag="VGB93_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0145314.1"
                     /translation="MRVDGKPTRTIWPAANGRGVMIIDQTLLPHEFVILELATLDDFA
                     VAIKDMKVRGAPLIGVTAAYGLAIALRDDPSDAGLAVASAKLLATRPTAVNLAWALNV
                     MRAALAPLPPVAWADAAFAKAGELADLDVAICDSIGVNGLTLIAKIAERKSRVNILTH
                     CNAGWLATVDWGTATAPIYKAQEAGIDLHVYVDETRPRNQGASLT"
BASE COUNT          426 a          767 c          723 g          477 t
ORIGIN      
        1 ttgctcgttt cgatctgtgt ggcgccagcc tttgcggcgg gaaccccgca gcaacgacgc
       61 gcctgtcgcg cagacgcctt tcgcttgtgc cgccaataca tcccttggga gcgcccggtc
      121 aaagcctgta tgattgcgaa cttttcgaaa ctcagcccgc tatgccgcgc acagtttcgc
      181 tccagccgca cctaacgctg aagttcgatg cggttaccta agcattcgaa gagacggaaa
      241 tgagattctt gaacatggtg tggcttggcg gtgttcgcgt tgttgctggc cgcgtatctc
      301 ttccgatacc agatcgtgga gacgcaaaac tttgtggtcg tcatggaccg ctggacgggc
      361 accatcagcc attgcgcagc cgcctattgc ggaagcaatc gtacagattg aaatgcttag
      421 aaggaccggc tcccccttcc ccgccgcgcc gtcgtgaaag ggaggcggac ggccgcttcc
      481 ctctagtcgt gctcgtaatc gacgtagccg cggcttgtca ccacgaccca gccatcgccg
      541 cgccgctcga tggcacgcac cgtgcccgca cctggcagcg tatcgcccgg catgacttcg
      601 acggccccct gcggcccttc gaccaaggca atgcctcgat aaacatcgcg cacgacccaa
      661 tcgttgagcg gttgcgatgc atggcggggc gcctcggcag aggcgagcgt gaccgggccg
      721 ggcgcggccg cattcgggat cgagccggtt gaatcgcccg acgctgtctc ggtccgtgcc
      781 gcgcgatcga gcgtcgcctg aatggcttga gcgtcgagac gcgcgacgcg cgccttttcg
      841 gccgtagcag cgtgggtatg gtcgagcttg gatgtcagtt gcgcaatctg cgccttgtaa
      901 tcggcttttt cggcctcgaa gccggctttc agcccatcaa gactcttctt gagactgcgc
      961 atctggtcgg gttcgccctg caaagccgca agacgcgcca gcaatttgtg aacttcgttg
     1021 ttgagcgcga ccgtctcgtg gcgaagcgca gcgagttgcg gctcatccgc actggcagat
     1081 gcggctaatg aagcgggccc cgccgccggc ggcgcaaccg cagccattgc cgcggtttgt
     1141 ggcgtcatcg cagcgacttg tatggggacg gacgcggacg gtgcagacaa ataatggctg
     1201 acgacagcat aggcgccccc tgcgataagc aggaccgccg cagcgcgcat ggcattattt
     1261 gtcagcgagc tccgtttcgt ccgcgccgga ccgccaaggc gctcgccggc gctgaagccg
     1321 ccgccgatct tttctttcgt cggcggcagg atgaccagcg ccgaaccggg aaatgattcg
     1381 acttccggcg gctctcggct ctcggcttcg ctcgcgctgg tcgcggcttt cggttcgaac
     1441 ttcgtcagcg cgtgcgacat gctattcgct tcgtccggcg cagcggatgc ggcgtccatc
     1501 gcgccttcag gcacagactc ggatttctta tcgtcgaaag cttgcggatc ggcagaggaa
     1561 tccatggcat gtgtcccggc agcagattaa gacattcgca acgttgcagg tgatttcgtt
     1621 aaaagcttgt tgaagaatcg gtatttcccc gtccggaggc ggcaggtttg cgacaaatcg
     1681 cacatttccg gcgcggcgct tgcttgccaa gcctgcccat gctttcgccg cacaaattct
     1741 cgactctccc cgccaaacca cattctttca ggatcttaga tgcgcgttga cggcaagccg
     1801 acccgtacga tctggccggc tgcgaacggc agaggagtga tgatcatcga tcagaccctc
     1861 ctgccgcacg aattcgtgat tttggaactc gccacgctcg acgactttgc cgtggccatc
     1921 aaggacatga aggttcgcgg cgcgccgctg atcggcgtca ccgctgccta tggcttggcg
     1981 attgctctac gcgacgaccc gagcgacgcc ggtcttgccg tggcaagtgc aaaactgctc
     2041 gcaacgcggc cgaccgccgt caacctcgcc tgggcgctca atgtcatgcg cgcggcgctg
     2101 gcgcctttgc cgccggtcgc atgggccgac gccgctttcg ccaaagcggg cgaactcgcc
     2161 gatctcgatg tcgcgatctg cgattcgatc ggcgtcaacg gcctcacgct catcgctaaa
     2221 atcgcagaac gcaaatcgcg cgtgaacatc ctgacccatt gcaacgccgg ctggcttgcg
     2281 acggtagatt ggggcacggc gaccgcgccg atctacaaag cacaggaagc cggcatcgac
     2341 ctccacgtct atgtcgatga gacgcggccg cgtaatcaag gcgcctcgct cac
//
LOCUS       DASWOG010000197         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_188073,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000197 DASWOG010000000
VERSION     DASWOG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188073"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGB93_04010"
     CDS             complement(<1..497)
                     /locus_tag="VGB93_04010"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0145315.1"
                     /translation="MKDLPLSEVYKLLEPGPVVLLTTARRGRHNIMTMSWHMMVDFTP
                     PLIACVVSNGDYSFAALRATKECVLAIPDVSLAEKVVKIGNCSGSDTDKFAEFALTPT
                     RAEKVSAPLIEECFANLECRVTDTRFVNKYGIFILEVVKAWKNPAKFDAKTIPHRGFG
                     TFAVDG"
     gene            complement(497..970)
                     /locus_tag="VGB93_04015"
     CDS             complement(497..970)
                     /locus_tag="VGB93_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145316.1"
                     /translation="MRLLLAAILALTVLPVRGDEVPFAQPAPSLDQHRQSYTVALSDA
                     MELTQLRHIKLWLAGNAKNWPLANYELTQLKDTFDKTAVLYLNIPVEYIASVEKPLGT
                     LADAVAAKDSVQFRRGFMALQTACNECHKAADVGFVVIQTPMVSVFPDEKFAPVR"
     gene            1072..1782
                     /gene="bluB"
                     /locus_tag="VGB93_04020"
     CDS             1072..1782
                     /gene="bluB"
                     /locus_tag="VGB93_04020"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEY0145317.1"
                     /translation="MRLAEHGDDAVDEADLAPAGAFSPEERAAVYRAIHARRDVRDEF
                     LPRPIPDAMLTRILEAAHHAPSVGLSQPWNFILIRDQATKTQVHDVFLRRNEEAAQMF
                     SETRGARYRQLKLEGIRKAPLNICVTCDRARGGRVVLGKTHQPDTDLYSTVCAIQNLW
                     LAARAEGIGVGWVSIFDPDDLRPILGIPAEIAVVAYLCVGFVDQLFVRPELEARQWAE
                     RLPLDDLVFEGTWGKMRG"
BASE COUNT          374 a          534 c          563 g          382 t
ORIGIN      
        1 ccgtcgacag cgaacgtgcc aaagccccga tgaggaatgg tctttgcatc gaattttgcc
       61 gggttcttcc aggctttgac aacttcaaga atgaaaatgc cgtatttgtt cacgaaccgt
      121 gtatcggtca cgcggcattc gagattggca aaacattcct cgatcagcgg tgctgagact
      181 ttctcagcac gcgtaggcgt caaagcgaat tccgcaaatt tatcggtgtc gcttccggag
      241 caattgccga tcttgacgac tttctccgcg agcgaaacgt ccggaattgc aaggacacat
      301 tcctttgtcg cccgcagcgc ggcgaaactg taatcgccat tactgacgac acacgcaatc
      361 aggggtggcg tgaagtcgac catcatatgc caggacatgg tcatgatgtt gtgccggccg
      421 cgccgcgcgg ttgtgagtaa gacaaccggg ccgggctcca gaagcttata gacctcggaa
      481 agaggtaggt ctttcatcac cgtactggcg cgaatttttc gtcggggaac accgatacca
      541 tcggggtctg gatgacgacg aagccaacat cggcggcctt gtgacactcg ttgcaggcgg
      601 tttgcagggc catgaagccg cgccgaaatt gcacggaatc tttcgccgcg acggcgtcgg
      661 cgagagtgcc aagcggcttt tcgaccgacg cgatatattc gacgggaata ttgagataaa
      721 gaaccgctgt cttgtcgaac gtgtccttaa gctgcgtaag ttcgtaattc gccagcggcc
      781 agttcttcgc gttgcccgcg agccaaagct tgatatggcg taactgggtc agttccatcg
      841 cgtcgctgag tgcgacggta taggattggc ggtgctgatc aagcgagggc gcgggctgcg
      901 caaacggaac ttcgtcgccg cgcacgggca aaacggtcaa cgcaagaata gccgcaagta
      961 ataatcgcat aggtgcctcc gtcgagccat tacgcagata aatggtccga tgcactttga
     1021 tccggatcaa gaacaatgca aaggtgcgcc cgactgtcag ggagaggcgc gatgcggctt
     1081 gccgaacacg gcgatgatgc agtggacgag gccgatctgg cgccggcggg cgccttctcg
     1141 cccgaggagc gcgcggccgt ctatcgcgcc attcatgccc gccgcgatgt gcgcgacgaa
     1201 tttctgccgc ggccaattcc cgacgcgatg ctcacgcgta ttctcgaagc tgcgcatcac
     1261 gcgccgtccg tcggcctcag tcagccgtgg aattttatcc tgatccgcga tcaggcgacg
     1321 aaaacgcaag ttcatgatgt gtttttgcgc cgcaacgagg aggcggcgca gatgttcagc
     1381 gagacacgcg gcgcacgcta tcggcaactc aagctcgaag gcattcgcaa agcgcctctg
     1441 aatatctgcg tcacctgcga tcgcgcgcgg ggcggccgcg tcgtcctcgg caagacgcat
     1501 cagcccgaca ctgaccttta tagtactgtc tgcgcgatcc agaatctttg gcttgccgcg
     1561 cgcgccgagg ggatcggcgt cggctgggtc agcattttcg accccgacga tttgcgtccg
     1621 atcctcggca ttccggcgga gatcgcggtc gtggcttatc tctgcgtcgg ctttgtcgat
     1681 cagctcttcg tccgtcccga gcttgaagcg cgccaatggg cggaacgtct gccgcttgac
     1741 gatctcgtct ttgaaggcac ctggggcaaa atgcgcgggt gaagcttagg cgcgccagtt
     1801 ggccgtgcgc gcgtgcggtg catcggccag cgccttccgc aacgccgctg cgc
//
LOCUS       DASWOG010000198         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_59407,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000198 DASWOG010000000
VERSION     DASWOG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59407"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGB93_04025"
     CDS             <1..824
                     /locus_tag="VGB93_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145318.1"
                     /translation="LDTARTIHSPGSRQAWSRWFRRVDATVNRGTNVLSGADNKPLLV
                     LSREDKGRVALLLSDQMWLWARGFQGGGPHLALLRRLAHWLMKEPELEEEALRATAQG
                     DELSIERQSLKDQIPPVRVFAPSGKEITATLKPAEPGLARADVKVSELGLYRVIDGSL
                     TTLVNVGPENPREFQEVVSTTEKLRPLAEATGGTVRRIGAAHGSDVSLPRIVAMHDSP
                     VYGGSDYAAIKRTDSAQVTGIGIAPLAIGFPGLLFLLASAVVTWLYEGRSRGARA"
     gene            complement(862..1314)
                     /locus_tag="VGB93_04030"
     CDS             complement(862..1314)
                     /locus_tag="VGB93_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0145319.1"
                     /translation="MLGGCCYAAYTLGANNTGNAVGVFYGLKLATPMKAGFAGGLVMA
                     LGALTWGRPILEKMGKGIVELGLDMGIGAKLAQSLTAHTAAICGYPTSMNQALIGGIA
                     GASRARGRETLNRRALTEIVFSWFFTPILAGVASFLLYVLVSKALGVR"
     gene            complement(1318..1833)
                     /locus_tag="VGB93_04035"
     CDS             complement(1318..1833)
                     /locus_tag="VGB93_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0145320.1"
                     /translation="METFLTTGMVLSGLFFGWTIGSHYTGATMGMAYGAGIIKNPMTA
                     LALIAVFVLLGATFASRHVVETVGAGIIPAQFMTPLGGMVMMLTAALVTAANTYMRWP
                     VSTSQLACFSVAGAGLGMGAPVNWSSTVFLLFVTWVGTPLISALLGFVFTRFIDLAVR
                     RPSEFKTRALR"
     gene            1954..3408
                     /locus_tag="VGB93_04040"
     CDS             1954..3408
                     /locus_tag="VGB93_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145321.1"
                     /translation="MLEQQEDRHPAMLRGIEVAFLIAIFAFLSMFHEIYVDDAFITLS
                     FARTFAESGVWGMHAPFVSNAATSPLNVILLSGLIKLGLPGIWAAYALECLLATAIFF
                     ALNFISRRVFGSRLWAYLATLILFTNPLLVSTSGLETYLFITVLLGCCIAYILERYLL
                     LGFAAGFLVLARPDGVLLLVPLLTLMIWERKRALDFGKVIIGFAIIVGVWGVVSWLYL
                     GSAIPDTYFIKRKEGAWGDISFAEGLALYYANCPLATFLSLAAAVFVPFALPLAKTNS
                     DWRRFVVLVGGLAFVHYAAYSLLRLPPYHWYYGILVGALGLIGSAGLVTLVPRARGPL
                     ALGLAAALALGSMATCAEAALAHGQMPTSTNWGTVRQYRAIAEWLNVHMPAAEYRMIG
                     ELGIIQYYGRADAVNSFSDRRILRDVLNRLPNGSIRKWLAEANFTHLVLRPPVRSVYL
                     LEENCKDSTGAVKTWTTSTTWTSTPIVWCLKREK"
     gene            complement(3410..>3997)
                     /locus_tag="VGB93_04045"
     CDS             complement(3410..>3997)
                     /locus_tag="VGB93_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0145322.1"
                     /translation="TRDDLVGQYIGHTAPKTKEVLKKAMGGVLFIDEAYYLYRPENER
                     DYGQEAIEILLQVMENQRDDLVVIFAGYAERMDKFFRANPGFRSRIAHHIDFPDYSDE
                     ELMSIAEMMLTSLSYHFDADGAAAFRDYIAARRKQPHFANARSIRNALDRMRLRQANR
                     HFERPGLVTLDDLTTISAQDVRASRVFSQDAPQSN"
BASE COUNT          742 a         1204 c         1203 g          848 t
ORIGIN      
        1 atttggacac agctcgaaca attcactcgc ctggctcacg gcaggcctgg agccgctggt
       61 tccgccgggt cgatgcgacg gtcaaccgcg gcaccaatgt cctgtccggt gctgataaca
      121 agccgctgct cgtactgtcg cgcgaggaca agggccgcgt tgcactgctt ctctcggatc
      181 agatgtggct ctgggcgcgc gggtttcagg gcggcggtcc gcatctcgcg ctgctgcggc
      241 gcctggcgca ctggctgatg aaggagccgg agctcgagga agaggctttg cgtgccacgg
      301 cgcagggcga cgaactctcg atcgaacgcc aaagcctgaa agatcagatt ccgcccgtgc
      361 gtgttttcgc gccgtccggc aaggaaatca cggcaacgct gaaaccggcc gagccaggcc
      421 tcgcgcgcgc ggacgtcaaa gtctcggaac tcgggctcta tcgcgtgatc gacgggtcgc
      481 tgaccacgct cgtcaatgtg gggccggaaa atccgcgcga atttcaggag gtcgtctcga
      541 caaccgagaa actgcggcca ctcgccgagg cgacgggcgg cacggtgcgc cgcatcggtg
      601 cagcgcatgg cagcgatgtt tccttgccgc ggatcgtcgc gatgcatgac agtccggttt
      661 atggcggctc ggattatgcg gcgatcaagc ggaccgattc cgcgcaggtc accggcatcg
      721 gcatcgcccc gctcgccatt gggtttccgg gcctgctgtt cctgctggcg agcgcagttg
      781 tgacctggct ttacgagggg cgcagccggg gcgcgcgggc ctagctagag catcggatca
      841 atccgatggg tcaatttcgg cttagcggac gccgagtgct ttcgacacca gcacgtaaag
      901 caggaacgac gcgacgccgg cgagaatcgg cgtgaagaac caggagaaga cgatctccgt
      961 gagggcgcgc cggttcaatg tttcgcggcc tctggcccgg ctagccccgg cgatgccgcc
     1021 gatgagcgcc tgattcatcg atgttgggta gccgcagatc gcggcggtat gggcagtcaa
     1081 gctctgcgcg agcttggcgc cgatgcccat atcaaggccg agctcgacga tgcctttacc
     1141 cattttttcg aggattggcc ggccccaggt cagggccccg agtgccatga cgagaccccc
     1201 ggcaaaaccg gccttcatcg gcgtcgcgag cttcaaaccg tagaagacgc cgacggcatt
     1261 gccggtattg ttcgcgccga gcgtataagc ggcgtagcaa cagccaccaa gcaatgctca
     1321 ccgcagcgcg cgcgttttga attccgaagg ccgtcgcacc gcgagatcga tgaaacgcgt
     1381 aaaaacgaaa ccgagcagcg cgctgatgag tggcgtgccg acccaggtca cgaagagcag
     1441 gaaaactgtg ctcgaccaat tgaccggcgc gcccatgccg agaccggcac cggcgacgga
     1501 aaaacaagcg agctgcgagg tcgaaaccgg ccatctcatg taggtattag cggccgttac
     1561 gagcgccgcg gtcaacatca tcaccatgcc gccgagcgga gtcatgaatt gagccgggat
     1621 gatacccgcg ccgactgtct caaccacatg cctgctcgcg aaggtcgcgc caagcaggac
     1681 gaagacggcg attaaagcca gcgccgtcat gggatttttg ataattccgg cgccgtaggc
     1741 catgcccatc gtcgcaccgg tgtaatgtga gccgatggtc cagccgaaga agaggccgga
     1801 gagcaccatg ccggtcgtca agaaggtttc catcctcgtc ccacagggct ttcggccaga
     1861 gccggttcgc aagccggtac ttaaggattt agtccattat acgcgcttta tagagaagcc
     1921 catcgtcggc gttatctgga gcggccattt ctcatgctag agcaacagga agatcgccat
     1981 cccgccatgc ttcgcgggat cgaagtcgca tttttgatag cgattttcgc atttctttcg
     2041 atgttccacg aaatctacgt cgacgatgcg ttcattacgc tctcctttgc gcggacattt
     2101 gccgaaagcg gcgtctgggg gatgcatgcg ccgttcgtct ccaatgcggc gacctcgcca
     2161 ctcaacgtca ttttgctcag cggacttatc aaacttgggc ttcctgggat ttgggcggcc
     2221 tacgctttgg aatgtcttct cgccaccgcg atcttcttcg cgctgaactt tatttcgcgg
     2281 cgcgtcttcg gctcgcggct ttgggcttat ttggcgactc tcatcttgtt caccaatccc
     2341 ctgcttgtct caacgtcagg cctcgaaacc tacctcttca tcaccgtcct gctcggctgc
     2401 tgcatcgcct atattctaga acgctatctg ttgctgggct ttgccgcagg gttcctcgtt
     2461 ttggcgcggc ccgatggcgt cttactgctc gtgccgctgc tgacactcat gatctgggag
     2521 aggaagcgag cgctcgactt cggcaaggtt atcatcggat tcgccattat cgtcggcgtc
     2581 tggggcgttg tttcctggct ttatctcggc tcggcgatcc ccgacaccta tttcatcaaa
     2641 cgtaaggaag gagcttgggg cgacatctca ttcgccgaag ggctcgcgct ttattatgcg
     2701 aattgtccgc ttgcgacgtt cctctccctg gcagccgccg tcttcgtccc tttcgctttg
     2761 ccgcttgcca agaccaatag cgactggcgc cgttttgtcg tgcttgtcgg cggtttggcg
     2821 ttcgtgcatt acgccgccta cagtctgctc cgcctgccgc cctatcattg gtattatggc
     2881 attctcgtcg gcgctttggg gcttatcggc tcggctggcc tcgtcacgct ggtgccacgc
     2941 gcgcgcggcc ctctcgcgct cggccttgcg gccgcgctgg cgctcggcag catggcgacc
     3001 tgcgccgagg cggcgctcgc acatgggcag atgccgacta gcaccaattg ggggacggtc
     3061 cgccaatatc gcgccatcgc cgaatggctc aacgttcata tgcccgcggc cgaatatcgg
     3121 atgatcggcg aactcggcat catccaatat tatggccgcg ctgatgcggt gaattcgttt
     3181 tcagaccggc ggattctccg cgatgtcctc aaccggttgc cgaatggatc aattcgcaaa
     3241 tggctcgccg aggcgaattt tacccatctc gtgctgcggc cgccagttcg ctccgtctat
     3301 ctgcttgagg aaaattgtaa agacagcact ggtgccgtga agacttggac gacttctacg
     3361 acatggacct cgacgccaat tgtctggtgc ctgaagcgcg aaaaataagc tagtttgact
     3421 gcggcgcgtc ttggctgaac acgcggctgg cacgtacatc ctgcgcagag atcgtcgtca
     3481 gatcgtcgag cgtgacgagg ccggggcgct caaaatggcg gttggcctgg cgcaatcgca
     3541 tccgatcgag cgcattgcga atcgaacgcg cgttggcgaa atgcggctgt ttgcgccgcg
     3601 cggcgatgta atcgcggaag gcggccgcgc cgtcggcatc gaaatgatag ctgagcgacg
     3661 tcagcatcat ttcggcaatg ctcatcagct cttcgtcgga ataatctgga aagtcgatat
     3721 gatgcgcgat gcgcgaacgg aagcccggat tggcgcggaa gaatttgtcc atcctttcgg
     3781 catagccggc gaaaatcacg accagatcgt cgcgctggtt ttccataacc tgcagcagga
     3841 tctctatcgc ctcctgcccg taatcgcgct cgttctccgg ccggtagaga taataggctt
     3901 catcgataaa cagcacaccg cccatcgctt ttttcaggac ttccttcgtc ttcggcgccg
     3961 tgtggccgat atattggccg acgagatcgt cgcgggt
//
LOCUS       DASWOG010000199         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_114654,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000199 DASWOG010000000
VERSION     DASWOG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114654"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..596)
                     /locus_tag="VGB93_04050"
                     /pseudo
     CDS             complement(1..596)
                     /locus_tag="VGB93_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764697.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(706..1580)
                     /locus_tag="VGB93_04055"
                     /pseudo
     CDS             complement(706..1580)
                     /locus_tag="VGB93_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087510.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
BASE COUNT          313 a          449 c          467 g          351 t
ORIGIN      
        1 ggagcgggcc ttcgtcgaca atgcgctgct tgcccacttt gccccatcgc ggcatctccg
       61 tcggatcgtc cacatcggtt gcaaagcagt gaacgagatt gcggggaccg aatttgtcaa
      121 tccaatcttg cgggtaatcg aaccaatagg ggtcagacat cgcgtcgagg tgataaagat
      181 acccgaaaaa ctcatcgaag ccgtgcacgg tcggcagata tttgttctta tctccaacat
      241 ggtttttgtc gaactgcccg gtcgtatagc ccatccattt aaggatggta gcaatcgtga
      301 tagcttgatc tggaatgccg atgtccgcgc ccggcttgtc ccaaagtggt catgccggta
      361 cgcaacggga tttggccggt gatgaaattt gcgcgacccg cggtgcagct tgcctccgcg
      421 taatagtccg tgaaccgcat gccgtcggcc gcgagcttgt caagattcgg cgtcttgccc
      481 gacatgatgc cttgatgata cgcgccgata ttgaaccagc cgacatcatc gcccatgatg
      541 acgagaatgt tcggttgctt gccgcttgat gcaggcggcg gctgcgcctg tgccatttgc
      601 ggtgcacctg atgtaacggc tgacgcggcg agcgccgtcc cggacagcaa gacgttgcgg
      661 cggttcacgc cctctcttgg cttttgcgct ttgtcagcct tatcgtcact ggaattcatc
      721 acgctctcct tttcgaaaga cgcatcggaa tccaagatga ctggttgaag tgttcaccgc
      781 ttccgcgagg cgcgcgtcgg gacgatagcg acggcaataa ttgggggcgc aaaggtgcga
      841 accacccttg atcactttgc gcgggatttt gatcttgggc tgcatgggat caaagctttc
      901 gtccgcccga ccgccgcgcg gattttccgg aatgcagcac gccttttgtg cgtccggccg
      961 atggctctgg gtgaaccaat cattggtcca ttcccagaca ttaccgatca tatcgtagag
     1021 gccgtaaccg ttgggcggaa atgctttgac gggaggcgtg cgggtcatat ccatcgagtt
     1081 tcaggttctc gcgtggaaat tcaccgtgcc atgtattggc catatatttg ccgccgggca
     1141 tgaactcgtc gccccaggcg tattccgcgc cgtcgagacc gccgcgggca gcgaactccc
     1201 attccgcctc ggtcggcaga tccttgccgg cccatttggc gtaggcgagc gcgtcaacat
     1261 gggcgatgtg aacgaccgga tggtcgccga gcccagcgat atcgctttcc ggcccataag
     1321 gatggcgcca atccgcacct ttcaagaagc gccaccattc gccccagtta cgcaaatcga
     1381 cacgatgcga gggcggcgtg aagacgagcg agcccgcata gagcatatgc ggcagcgcac
     1441 ccgggtaatc tttcgggtcg ggcacgattt cggcgaatgt gaggtgatgg gtcgcgcgga
     1501 tgaattcgtt aaattgctgg ttggtaaccg gcgccgcgtc gatccaaaag ctatcgacgg
     1561 ccacgcgatg aaccggcatt
//
LOCUS       DASWOG010000200         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_242707,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000200 DASWOG010000000
VERSION     DASWOG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242707"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VGB93_04060"
     CDS             <1..681
                     /locus_tag="VGB93_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY0145323.1"
                     /translation="AVHPETKAPIVAGIGRYGPYVQHGKTYANIGKDEDILSIGGNRA
                     IDLIVAKESGLSGRRFGAEASSASRELGDHPQGGAVVIKAGRFGPYVNWGKINATLPR
                     ESDPTSVTLDDAIALLAAKAQGGGGGGGTRGRVLGQHPSGGDIVLREGRFGPYVSLGK
                     VNATLKNGAAADSITLEDAIQLIDAKGGVPTKKKAAAKAKPAKAAPKKAAAKKTATKA
                     PARKAKRA"
     gene            681..>2296
                     /locus_tag="VGB93_04065"
     CDS             681..>2296
                     /locus_tag="VGB93_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY0145324.1"
                     /translation="MKKAGSAEAGLSGAKAERPKGALPSKAEILAFIGREQPNSPTKI
                     GKREVARAFSITGADRIGLKKILRELEAEGAVGRNRKQLHKAGQLPAVVLADVTSRDP
                     DGELIAQPVEWDNEQGPIPKILIHVGKRRPGQSVPGVGDRGLIRVEPAYEAGPNDPAY
                     NGRVIKLFDRAKTQVLGIYRADPAGGARVVPIDKRNARIGEFTVAEGGHNGAQEGDLV
                     AVDVLRTGRLGLAPVRVREKLGSLASEKAMSLIAIHSHSIPNAFSSDALAEASHAKPA
                     TLAGREDWRDIPLITIDPADAKDHDDAVFAQPDRNPDNDGGHIIHVAIADVAAYVRPG
                     SALDRDALQRGNSVYFPDRVVPMLPERISNDLCSLRPREDRAALAFRIIIAKDGRVVS
                     HNVHRVLMRSAAKLAYQQVQAAWDGTPGEETQALLKPVLKPLFAAYESLKLAREKRNP
                     LDLDLPERKLLLKPDGTVDRVIVPPRLEAHRLIEEFMILANVAAAETLEKVHQALIYR
                     AHGEPTFEKLNALAEFLASIDIKIAKGQVLT"
BASE COUNT          483 a          730 c          703 g          380 t
ORIGIN      
        1 gccgttcatc ccgagaccaa agcgccgatc gttgccggca tcggccgtta cgggccctat
       61 gttcagcacg gtaagaccta cgccaatatc ggcaaggacg aggatattct ttcgattggc
      121 ggcaatcgcg ccatcgatct catcgtcgcc aaggagagcg gtctttcggg ccggcgcttc
      181 ggcgcagagg cgtcgagcgc gagccgcgaa ctgggcgatc atccgcaagg cggcgccgtg
      241 gtcatcaagg ccggccggtt cggcccctac gtcaattggg gcaagatcaa tgcgacgcta
      301 ccgcgcgagt ctgacccgac gagtgtgacg ctcgacgatg cgatcgccct gctcgccgcc
      361 aaggcgcaag gaggcggcgg cggtggcggg acgcggggcc gcgtgctcgg gcagcacccg
      421 tcaggcggcg atatcgttct gcgcgagggc cgatttggac cttatgtcag cctcggcaag
      481 gtcaatgcga ccttgaagaa cggcgcggcg gcggattcga tcacgctcga agacgcgatc
      541 cagttgatcg atgccaaggg cggtgtccca acgaagaaga aggccgcggc gaaagccaag
      601 cctgcaaagg ccgcgcccaa aaaagccgcg gcgaagaaaa ccgcaaccaa agcgcccgca
      661 cggaaggcga agagagcgtg attaagaaag ccggctcggc agaagccggc ctgtccggcg
      721 cgaaagccga acgaccgaaa ggtgctctgc cttcgaaggc agagattctc gccttcattg
      781 gccgcgaaca accaaacagc cccaccaaaa ttggcaagcg ggaagtcgcc cgcgctttct
      841 cgatcaccgg ggcagatcgc ataggattaa agaaaatact cagagaactc gaagcggaag
      901 gcgccgttgg gcgcaaccgc aaacaattac ataaggccgg tcaattgccg gctgtcgtcc
      961 tcgccgatgt cacaagccgc gacccagacg gcgaattgat tgcccagccc gtcgaatggg
     1021 acaatgaaca agggccgatc cccaaaattc tcatccacgt cggcaagcga cgccccggcc
     1081 agtcggtgcc cggcgtcggc gaccgcgggc ttattcgcgt cgaaccggcg tacgaagccg
     1141 gtccgaatga tcccgcctat aacggccgcg tcatcaaact tttcgaccgc gctaagacgc
     1201 aagtgctcgg tatctatcgc gccgacccgg ccggcggcgc gcgtgtcgtg ccgatcgaca
     1261 aacgcaatgc ccgcatcggt gaattcaccg tcgccgaagg tggccacaat ggtgcgcagg
     1321 aaggcgatct cgtcgccgtc gatgttttgc gcaccggacg tcttggcctt gcgcccgtgc
     1381 gcgtgcgcga aaagcttggc agcctcgcga gcgaaaaggc gatgagcctc atcgccatcc
     1441 attcgcacag tattcccaat gctttcagtt cggacgcttt ggcggaagcc tcccacgcga
     1501 agccggcgac gctcgccggg cgcgaggact ggcgcgacat tcccctgatc accatcgacc
     1561 cggccgacgc caaggatcac gacgacgcgg tgttcgcgca acctgatcgc aaccccgata
     1621 acgacggcgg ccatatcatc catgtcgcta tcgccgatgt cgcggcctat gtgcggccag
     1681 gctctgcgct tgaccgcgat gcactccagc gtggcaattc ggtctatttc ccggatcgcg
     1741 tcgtgccgat gctgcccgag cgcatctcca acgatctttg ttcgctgcgg cccagagaag
     1801 atcgcgccgc gctcgccttc cgtatcatca tcgccaaaga tggccgcgtg gtatcgcaca
     1861 acgtgcatcg cgtgctgatg cgctcggcgg cgaagcttgc ctatcagcaa gtgcaagccg
     1921 cctgggacgg aacgcccggc gaagaaacgc aggcgctgtt aaagccggtg ctgaagccgc
     1981 tctttgctgc ttatgaatca ttgaagctcg cgcgcgaaaa gcgcaacccg ctcgatctcg
     2041 atctgccgga acgcaagctt ctgctcaagc ccgatgggac cgtcgatcgc gtgatcgtgc
     2101 cgccgcggct tgaagcgcat cggctgatcg aggaattcat gatcctcgcc aatgtcgccg
     2161 cggcagagac gttggaaaaa gtgcatcagg ccctgatcta tcgcgcgcat ggcgagccga
     2221 cattcgagaa gcttaatgcg ctcgctgaat tcctcgcctc gatcgacatc aagatcgcga
     2281 agggccaagt gctgac
//
LOCUS       DASWOG010000201         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_242728,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000201 DASWOG010000000
VERSION     DASWOG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242728"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VGB93_04070"
     CDS             <1..302
                     /locus_tag="VGB93_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="modification methylase"
                     /protein_id="HEY0145325.1"
                     /translation="QPRVAFASVVEAGLIAPGTILVDEKKRHRAFVRADGTLALAGAS
                     SQLAIVGSIHKVGALAQGLPACNGWTFWHIELGGKLAPIDELRSTIRDALKTAAE"
     gene            complement(350..904)
                     /locus_tag="VGB93_04075"
     CDS             complement(350..904)
                     /locus_tag="VGB93_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0145326.1"
                     /translation="MTQATLRMAWAQRLRSADLAAVHLISRSARPKIGRYSAIAISKL
                     GNSWLYLIILPVIFAALGREGFRVVFLGALNAALLHLMFPIIKRKIGRPRPYQVDPRL
                     RSLLAVLDEHSFPSGHTMTLSGVLTPIVLVWPGAILSAVALLILMAWSRIATAHHYPS
                     DIFGGAILGIVLAYPLSILVLAIW"
     gene            complement(1108..1527)
                     /locus_tag="VGB93_04080"
     CDS             complement(1108..1527)
                     /locus_tag="VGB93_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145327.1"
                     /translation="MKMPLLATLFVNTDSSASGDAPGLRDLILGLHSGSISAAVVLDR
                     LDPGFADEIRAFVVHRGLTAENFIANALLAFALDVADQSWRQGLKSRQPHSDDPEADM
                     VADLLREIMRQRRDREIHLASPAAGRESHVTLGRRRN"
BASE COUNT          361 a          482 c          523 g          302 t
ORIGIN      
        1 tgcagccgcg tgtcgccttc gcgtcggtcg tcgaagccgg cttgattgcg cccggaacaa
       61 tccttgtcga cgaaaagaag cgccatcgtg ccttcgttcg cgccgacggc actttggccc
      121 ttgcaggtgc aagctcgcaa ctcgcgatcg tcggctcgat tcacaaagtc ggcgctcttg
      181 cacagggcct gccagcctgt aacggctgga ccttctggca tatcgaactg ggcggcaagc
      241 tcgcgcctat cgacgaattg cgcagcacga tccgcgacgc cctgaaaacc gcagcggaat
      301 agagtatcgg acagaaacta ctacggtttt cgagggccga tgtcgtgatc taccagatcg
      361 cgagaacgag aatcgagagc gggtaggcga gcacgatgcc gagaatggcg ccgccgaaaa
      421 tgtcgctcgg gtaatgatgc gccgtggcga tgcgcgacca cgccatcagg attagcaggg
      481 caacggcaga gagaatcgcg ccgggccaga cgaggacgat cggcgtcagc acgccggaca
      541 acgtcatcgt atggccgctc ggaaaggaat gttcgtcgag gacagcgagc agggaccgga
      601 ggcgcggatc gacctgatag ggccgcggcc gtccgatctt gcgcttgatg atgggaaaca
      661 tcaggtgcag cagcgcggcg ttcagcgcgc cgaggaatac gacgcggaac ccctcgcgac
      721 cgagagccgc gaagatcacc ggcagaatga tgagataaag ccagctattg ccaagcttac
      781 tgatcgcgat ggccgagtaa cggccgatct tcggccgggc cgaacgcgaa atcagatgga
      841 ccgcggcgag atcggcgctg cgcaatctct gggcccatgc catacgaagt gtggcctgtg
      901 tcattggcgg tcgatcgatt ggctctggcg tcgaaacatt gaccataacc actccgggcg
      961 acccgatcag ccatcgtctg ccttaccaga cgggaagaca atgaccgcca tatgacatta
     1021 tcgcggttct tcacgccttg atcgcattgc agcgagtgcg tcgcaaaatg gcaacaaaat
     1081 caaatgtggg ccgcctgaat ctcgcgatta attgcggcgg cggccgagtg tcacatgcga
     1141 ttcgcgcccc gcggccggcg acgcgagatg aatctcgcga tcgcgccgct ggcgcataat
     1201 ttcgcgcagc agatccgcaa ccatatcagc ttccggatcg tcgctgtggg gttggcggct
     1261 tttgagtcct tgtcgccagg attgatcggc gacgtcgaga gcaaaggcca gcaatgcatt
     1321 ggcgatgaag ttttctgccg taagtccgcg atggacgaca aaggcgcgaa tttcatcggc
     1381 aaagccggga tcgagcctgt cgagcacgac ggcggctgaa atgctgcctg aatgcagccc
     1441 caggatcagg tcgcgcaggc ccggcgcatc gcccgaggcg gatgaatcgg tgttgacgaa
     1501 caatgtcgcg aggagcggca ttttcatgca aatctccggg gcctgacttc caccccggag
     1561 tatagcggat tttttgtcgt ctgtacgaac ccgatcaacg attcgtactt ctggcgcggc
     1621 gaaacggcct atccgaataa tttcgtcgcg acttcggcga cgtgccgc
//
LOCUS       DASWOG010000202         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_261357,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000202 DASWOG010000000
VERSION     DASWOG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261357"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VGB93_04085"
     CDS             complement(<1..1352)
                     /locus_tag="VGB93_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY0145328.1"
                     /translation="MADVERTQGTAALLSLADKYDLAQNRVFVSGPQAIVRLLLMQAA
                     IDRRDGLNTAGFISGYRGSPLGGLDLNFLRAKKALTACDIIFEPGLNEDLAATAIWGA
                     QQAEMRGEGRFDGVFGLWYGKGPGVDRSGDVFRHVNLAGTSRNGGVLALMGDDHTAES
                     SSTAHQSEFTFVAMMMPILAPAGVQELIDYGLYGYALSRYSGCWVGIKCLKDTVESTA
                     SVDASLDRVRPVVPTDFILPAGGLNIRPHDPVLAQERRLQNEKFPAVLAWLRANKLNR
                     IITSGGPQAKLCIVASGKSYLDVRQALDELGIDEVRANALGLRLYKVGCPWPLEPQGL
                     RTFAQGLDKIIVVEEKRSLIENQLRDELYGSAHQPVCVGKKDEKGRQLFPVAGALDVN
                     DIAIAIGRRLLDYHSDAELESRVAHLTNLQFGITTLKDAATRTPYFCSGCPHNVSTKV
                     P"
     gene            1624..>1955
                     /locus_tag="VGB93_04090"
     CDS             1624..>1955
                     /locus_tag="VGB93_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HEY0145329.1"
                     /translation="MRASKRLKFGVAAFLLGATAPASAHPHVWVTAHEDVIFQPDGKI
                     AAIRNSWVFDDMYSAFAVQGLAKDGQLATKEQLAPLAKTNVESLAVFGYFTFAKNAGA
                     KTDFGPPK"
BASE COUNT          375 a          600 c          610 g          370 t
ORIGIN      
        1 ggcactttcg tcgagacgtt atgcgggcag ccggagcaga aatagggcgt ccgcgtcgcc
       61 gcgtctttca gtgtcgtgat tccaaactgc agattcgtga gatgggcgac acggctttcg
      121 agctcagcgt cggaatgata gtcaagcagc cgccgcccga tggcgatggc gatatcgttg
      181 acatcgagcg cgccagcgac ggggaacagc tgacgacctt tctcgtcctt cttgccgacg
      241 cagaccggct gatgcgcgct cccataaagc tcgtcgcgca actggttctc gatgagcgag
      301 cgcttctcct cgacgacgat gatcttgtcg agaccctgcg cgaacgtccg caggccctgc
      361 ggctccagcg gccaggggca gccgaccttg taaagccgca ggccaagcgc attggcgcgc
      421 acttcgtcga tgcccaattc atcgagcgcc tgacgcacat cgagatagga tttacccgac
      481 gcgacaatgc agagcttcgc ctgcggacca ccagaggtga tgatccgatt cagtttgttg
      541 gcgcgcagcc acgccagcac ggccggaaac ttttcgtttt gcagccgccg ttcctgcgca
      601 agcacaggat cgtgcgggcg gatgttgaga ccgcccgccg gaagaatgaa atctgtcggc
      661 acgacgggcc gcacgcggtc gagagaggca tcgactgagg ccgtcgattc gaccgtgtcc
      721 ttcaggcatt tgatgccgac ccagcagccg gaatagcggc tcagcgcata gccgtagagg
      781 ccgtaatcga tcaactcctg cacgccggcc ggcgccagga ttggcatcat catcgcgacg
      841 aaggtaaatt cggactgatg tgccgtgctc gaggattcgg ccgtatggtc gtcgcccatc
      901 agggcaagga cgccgccgtt gcgcgaggtg ccggcgaggt tgacgtggcg gaagacatcg
      961 ccggagcgat ctacgcccgg ccccttgcca taccacaggc cgaaaacgcc atcgaaccgg
     1021 ccttcgccgc gcatttcggc ctgctgcgcg ccccagatcg cggtcgcggc aaggtcttca
     1081 ttgaggccag gttcgaaaat gatatcgcac gccgtcagcg cttttttggc gcgcaggaaa
     1141 ttgaggtcga gaccaccgag cggcgaaccg cggtagccgg agatgaagcc ggcagtgttc
     1201 agtccgtccc ggcggtcgat cgccgcctgc atcagcaaaa ggcggacaat agcctgcggc
     1261 cccgagacga agacacggtt ttgggcaagg tcgtatttgt cggcgagcga cagcagcgcg
     1321 gccgtgccct gggttctttc gacgtccgcc attttgcctt tgcccccagg caggaagatc
     1381 cagcctggcc ccattgggag taaacggtat aaagcagccg gttgaatcgg ccaacccttt
     1441 aggcggctgc gccgcgccgt ctcccctgcg tttaagtaaa tgattgtaac cgtgttttgc
     1501 gggatggacc gccccgctgg ggcgacgaag tgatcgcccc tgcctcgctt tggccagagt
     1561 ttgccgggca attgttgccc cgacgattct gccattccgg ccttaggcct ttgatcggga
     1621 aagatgcgcg cctcaaaaag gcttaaattt ggcgttgcgg cctttttgct cggcgcgacg
     1681 gctccagcga gcgcgcatcc ccatgtttgg gtaacggcgc acgaggatgt gatcttccag
     1741 ccggacggca agatcgcggc gatccgtaat tcctgggtgt ttgacgacat gtattcggcc
     1801 ttcgccgtcc agggcctcgc caaggacggc caattggcga ccaaggagca gctcgcgccg
     1861 ctcgccaaga ccaatgtcga atcactggcc gtgttcggct atttcacctt cgcgaagaac
     1921 gccggcgcga aaaccgattt cgggccgccg aaaga
//
LOCUS       DASWOG010000203         5103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_133083,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000203 DASWOG010000000
VERSION     DASWOG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133083"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGB93_04095"
     CDS             complement(<1..642)
                     /locus_tag="VGB93_04095"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0145330.1"
                     /translation="MAKAFVFPGQGSQAVGMGKALAEAYAPARQVFAEVDEALHQKLS
                     ALMFEGPEAELTLTANAQPALMATSLAVLRVLEAEAELDLGRDAAFVAGHSLGEYSAL
                     AAAGSFSIPDAARLLRRRGEAMQQAVPVGVGAMAALIGLDLDGAIEVAKAAAEQTGEV
                     CAVANDNGGGQVVISGTKVAVDAAIEIAKGKGVRRAVPLAVSAPFHCPLMQPAA"
     gene            820..1236
                     /locus_tag="VGB93_04100"
     CDS             820..1236
                     /locus_tag="VGB93_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145331.1"
                     /translation="MDIPGKACGTCSMCCKILEIEELTKPAGPYCPNCKLGDGCGIYH
                     SRPDVCRDYECLWKTDRTLSPQLRPDRSGTILMEDADSEQYQAVCDPDKPLAWRNNPL
                     IFKHLVAVAKSGRTVVAKAGVKAWRIRASGEWGPSA"
     gene            1369..1587
                     /locus_tag="VGB93_04105"
     CDS             1369..1587
                     /locus_tag="VGB93_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY0145332.1"
                     /translation="MRKISILVLCAALAGFGASPVLAQSSGTSSTMAAKPAHTAKSLE
                     CSKEADAKGLHGKARKHFRSACKHGKSM"
     gene            complement(1618..1899)
                     /locus_tag="VGB93_04110"
     CDS             complement(1618..1899)
                     /locus_tag="VGB93_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145333.1"
                     /translation="MPYATDKILRIAFAAFLGGAAAAPALAQSGNGYGLSGLKSYDAP
                     LAPANGLARVIRSENQSPTGMPRKFKPDIGPAVTVHAFGEVIVDSTMRR"
     gene            2226..2672
                     /gene="rpsF"
                     /locus_tag="VGB93_04115"
     CDS             2226..2672
                     /gene="rpsF"
                     /locus_tag="VGB93_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0145334.1"
                     /translation="MALYEHVFLARQDASAQQVEALTEQFKGVIEAHGGKIEKTEYWG
                     VKSVAYRIKKNRKAHFTLLNIDAPAAALTEVERQESLNEDVLRFLTVRVEELEAGPSA
                     MLRKRDDDDRGDRGDRGRGPRPDRGDRGDRGPRRPRDEAGVDEGAF"
     gene            2672..2920
                     /gene="rpsR"
                     /locus_tag="VGB93_04120"
     CDS             2672..2920
                     /gene="rpsR"
                     /locus_tag="VGB93_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0145335.1"
                     /translation="MATTTASPARRPFFRRRKTCPFSGPNAPKIDYKDTRLLSRYISE
                     RGKIVPSRITAVSAKKQRELAKAIKRSRFLGLLPYVIR"
     gene            3073..4137
                     /locus_tag="VGB93_04125"
     CDS             3073..4137
                     /locus_tag="VGB93_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145336.1"
                     /translation="MVNKQKQKQTSDIRGAVAFGSGLAATLLLLAAFQGSVPALLLAY
                     LSPLPLMIATIGFGHVTGLGAVLAGFITLVALLVAANPQDVSVATFAGAGVNGILFLI
                     CEGLPSWWLARVASVSRSMPILPFLPEPPPDAVEEGSRLSLVIVTAAAFAFLVVGGTL
                     ATMVLGHEDFAALVAKVAANIRPVLKQEAGADLLHANAIDGFAHFLATALPAIAAGVI
                     FVIYLINLWLAGRVVQISQRLQVPWPNVAREFRVPRLLALVFVAAAGLIFVGGAIGLV
                     ASLAASVLGVAFALQGLAVIHVATLGLRLRHNLLFFVYLLVVPLMPWSLAPFTLLGLL
                     EAGFSLRDRKDSAAISPKMN"
     gene            4150..4713
                     /gene="rplI"
                     /locus_tag="VGB93_04130"
     CDS             4150..4713
                     /gene="rplI"
                     /locus_tag="VGB93_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0145337.1"
                     /translation="MDVILLQRVAKLGQMGETVRVKNGYARNFLLPTGKALRATEANK
                     KRFQDQKADLEARNLHERGEASSVAEKLGGQSFVIVRQAGETGQLYGSVSARDVADAV
                     SAGGFKVSREQIELVAPMKTLGLHTVPVHLHPEVEAKVTINIARSPEEAERQAKGEAI
                     NVVEETTIQDLGLEVGAALAEAGGDED"
BASE COUNT          985 a         1609 c         1498 g         1011 t
ORIGIN      
        1 ggcggcaggc tgcatcaaag gacaatggaa tggcgccgat acggcaaggg gaacggcgcg
       61 gcgcacgccc ttgcccttgg caatttcgat cgccgcatcg acggccactt tagtgccgga
      121 aataacgacc tggccgccgc cattgtcatt tgccacagca cagacttcgc cggtttgttc
      181 cgccgcggct ttcgccacct caatcgcccc gtcgagatcg agaccgatca gagccgccat
      241 cgcgccaacg ccaaccggaa cggcctgctg catggcctcg ccacgccgcc gcagcaatcg
      301 cgcggcatca ggaatcgaaa aagaaccggc ggccgcgagc gcagaatatt cgccaagcga
      361 atggccggcg acaaacgccg catcgcggcc aagatcgagt tccgcttccg cctcgagcac
      421 gcgtaaaacc gcgaggctcg tggccatcaa cgcgggttgc gcgttcgcgg tcaaagtcag
      481 ttcggcctcc ggcccttcga acatcaatgc cgagagcttt tgatgcaggg cctcatcgac
      541 ttccgcaaaa acctgccgcg ccggcgcata agcttcggcg agcgctttgc ccatgccgac
      601 ggcctgcgaa ccctgacccg gaaatacaaa tgctttcgcc atacgcgcgc ttccgctcta
      661 ttactttagc tgttctcggc gctgccgact gacaccggtt aacgggcaag tcaagtttct
      721 ggtgacggcg cggcgcccgg tggaatttct attgcctccg cgatgcagca cgccatgatg
      781 ccgaatctgc gatcgatcgt aggcggagcg aacctcaata tggacattcc aggcaaagcc
      841 tgtggcacct gcagtatgtg ctgtaaaata cttgagatcg aagaattgac gaagccggcg
      901 ggcccttatt gcccgaattg caagctcggc gacggctgcg gcatctatca ttcgcgtcca
      961 gatgtttgcc gcgactatga gtgcctgtgg aagaccgatc gcacccttag cccgcagctg
     1021 cggccggacc gcagcggcac aatcctcatg gaggatgcgg attccgaaca atatcaggct
     1081 gtttgcgacc ccgataaacc tctcgcctgg cgcaacaatc cgctgatctt caaacatctc
     1141 gtcgctgtcg ccaagtctgg tcgcaccgtc gtcgccaagg ctggcgtgaa ggcttggcgg
     1201 attcgcgcga gtggcgaatg gggaccgagc gcttaagcac aagcatggcg gcacgctcgt
     1261 ggattgcacc ttcgtacgac ctcgacttca tgatgcattc atttacggga tgaaattgtc
     1321 cgcctcccaa tcagtggcaa acgaaagcca ttcaacgagg agggtttaat gagaaaaatt
     1381 tccattctcg tgctttgtgc tgccctcgcc ggcttcggcg catcgccggt tctcgcgcaa
     1441 agctccggca cgtcgtcgac gatggcggcg aagccggcgc ataccgctaa atcgctcgaa
     1501 tgctcaaaag aggctgacgc caagggcctt cacggcaagg cgcgcaagca cttccgctcg
     1561 gcgtgcaagc acggcaagag catgtaattc ggccgggcgg cggctccgcg ccaccgccta
     1621 ccggcgcatc gtgctatcga cgatcacttc gccaaaggca tgcacggtga cggcgggccc
     1681 gatatccggc ttgaatttcc gcggcatccc ggtcggactc tgattttccg accggatcac
     1741 tctggcaagg ccgttggctg gcgccaacgg tgcatcataa gatttcaaac ccgatagacc
     1801 atagccattc ccgctctgcg ccagagccgg agcggcggca gctccaccga gaaaagcggc
     1861 gaaagcgatt ctgaggattt tgtcggtcgc ataaggcatg caccaacata gcaacccgct
     1921 gaagggccgc ctatagaatc ggcatcgccc tgggcgcaag atttcttgat ttcgccgccg
     1981 ttctgcgtat aaagcccctt cccgcgggca tatggctcgc ggacagcggc ttcggccggg
     2041 ggctgtacgg aaggcgtgtc gcgcctcatc ccaaaaagtt gcaagacttt ttggaccaga
     2101 tgatgcggaa acgatacgca gaatttctcg aaaattcgtt ttcccgtgtc tccgccttcg
     2161 ttcgttcgag cctttccggg gctcgaaggg ctctgcgccg gccgcgcaac aggaaaggca
     2221 agatcatggc tctttatgag catgtatttc ttgctcgtca ggatgcgtcc gcccagcagg
     2281 tggaagcttt gaccgagcaa ttcaaaggcg tcatcgaggc gcacggcggc aagatcgaga
     2341 agaccgaata ttggggcgtc aaatccgtcg cctatcggat caagaagaac cgcaaagcgc
     2401 atttcaccct gctgaacatt gatgcgccag ccgctgctct gaccgaggtc gagcgccagg
     2461 aaagcctcaa cgaagatgtg ctgcgcttct tgactgtccg tgtcgaagaa ctcgaggctg
     2521 gaccctcggc catgctgcgt aagcgcgacg acgacgatcg cggtgaccgt ggcgatcgcg
     2581 gccgtggtcc ccgcccggat cgtggcgacc gtggtgatag aggcccgcgc cgtccgcgcg
     2641 acgaggccgg tgtggacgaa ggagctttct gatggcaacc accacagcat cgccagcccg
     2701 gcgccccttc ttccgccgtc gcaagacctg cccgttctcc ggccccaacg cgccgaagat
     2761 cgattacaag gatacgcgac tcctttcccg ctacatttcc gagcgcggca agatcgtgcc
     2821 ttcgcgcatc acggcggttt ccgccaagaa acagcgcgaa ctggcgaagg cgatcaagcg
     2881 gtcccggttc cttgggcttc tgccctacgt gatccggtaa ggtctttcgt tttgacgtgt
     2941 tttctttacg cgaactctta atccacttcg cctgaaaaca ctttaacacg cgacgcataa
     3001 agccgccggc acagcccggc ggctcagctg ggagaaagca ctccctaacc gcgaaggcgg
     3061 gacagctcgg cgatggtcaa caaacaaaag caaaagcaaa catcggatat tcgcggtgcg
     3121 gtcgccttcg gcagcggcct cgccgcgacg ctgcttttgc tcgccgcctt ccagggctcc
     3181 gtgcccgctt tgctgctcgc ctatctctcg ccgctgccac tgatgatcgc gacgatcggc
     3241 ttcggccatg tcaccggcct tggcgcggtg ctcgccggct ttatcacgct cgtcgctttg
     3301 ctcgtcgccg ccaatcccca ggatgtgagc gtcgccacgt tcgcgggcgc cggcgtcaat
     3361 ggcatcctct ttcttatctg cgaggggctg ccctcgtggt ggctggcgcg ggtcgccagc
     3421 gtctcgcgca gcatgccgat cctgccgttt ctgcctgaac cgccacccga tgcggtcgag
     3481 gaaggttcgc ggctgagcct ggtcatcgtg acagcggccg ccttcgcatt tcttgtcgtc
     3541 ggcggaacgc tcgccacgat ggtcctcgga cacgaggatt tcgccgcttt ggtcgccaaa
     3601 gtggctgcaa atatccgccc ggtcctgaaa caggaagccg gcgcggattt gctgcatgca
     3661 aacgcaatcg atggctttgc ccatttcctc gcgacagcac tgccggccat cgccgccggc
     3721 gtgatcttcg tgatctatct gatcaatctc tggcttgccg gcagggtcgt acagatttcg
     3781 cagcgcctgc aggtcccgtg gccgaacgtg gcccgcgaat ttcgcgtgcc gcgcctgctc
     3841 gcgctcgtat tcgttgccgc ggccgggctg atctttgtcg gtggcgcaat tggccttgtt
     3901 gccagccttg cggcgagtgt gctcggcgtc gctttcgcgc tccaaggtct cgccgtcatc
     3961 catgtggcga cgcttggtct gcggctgcgc cacaatctgc ttttttttgt ctacctcctc
     4021 gtggtgccgc tcatgccgtg gtctttggca cctttcactt tgcttggcct gcttgaggcc
     4081 ggcttttcgc tgcgcgaccg gaaagattcg gccgccattt cccccaagat gaactgaagg
     4141 aattttctta tggacgttat cctgctgcaa cgtgtcgcca aattaggcca gatgggcgaa
     4201 acggttcgcg tgaagaatgg ctatgcacgc aatttccttt tgccgaccgg caaggctctg
     4261 cgcgccacgg aagcgaacaa gaagcgcttc caggatcaga aggccgatct ggaagcccgc
     4321 aatctgcacg aaaggggcga ggcgagctca gtcgccgaga agctcggcgg ccagagcttc
     4381 gtcatcgtcc gccaggcggg tgagacaggc cagctttacg gctcggtctc ggcccgcgac
     4441 gttgccgacg cggtcagcgc cggcggcttc aaggtctccc gcgagcagat cgaacttgtc
     4501 gcgccgatga agacgctggg cctgcatact gtgcctgtcc atctgcatcc ggaagtcgag
     4561 gcgaaggtca cgatcaacat cgcccgttcg ccggaagaag cagagcgcca ggccaagggc
     4621 gaagcgatca acgtcgtcga ggaaaccacc atccaggatc tcggcctcga agtcggcgcg
     4681 gccttggcag aagcgggcgg cgacgaggac tagccgcact cacggcgttg cccgtcatgc
     4741 ccgggcttgt gccggacatc cacgccctgc agaacttatg agtcccacgt ggatggccgg
     4801 gacaagcccg gccatgacgg cgctcttgca aatccgtgcg acggatcaat gggatcgaaa
     4861 agtccagcgc cgtgcaggcc acgactgtag aatcttttcg ttttgtggat tgcgggacgc
     4921 gcccctcggc tggttgaatt tgattccaat ctgagcggag cacgtgccaa aaaggcggct
     4981 tccgaatttc tcgcgacagt gattcgctaa gataatcatg gccgccaatt catcgactgc
     5041 tctcgccacc acccaaagca atgatcccgc ctatcgggtg ccgccgcata atatcgaggc
     5101 gga
//
LOCUS       DASWOG010000204         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_114744,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000204 DASWOG010000000
VERSION     DASWOG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114744"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..874)
                     /locus_tag="VGB93_04135"
     CDS             complement(53..874)
                     /locus_tag="VGB93_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEY0145338.1"
                     /translation="MEATRNRAGQRYGLENFAFPERGNETLDLLFSHRSVRAYLPDRL
                     PEGTLEVLVTAAQSASSSSNLQAWSVVGVEDKDRKARLAALANDQKHIIRAPLFLVWL
                     LDLARLNELAKERGLLAEGLDYIESFLVGAVDTSLAAQNAVVAAESLGLGTVYIGAIR
                     NKPAEVAKELGLPAHVFPLFGLVVGHPDPGVPTGVKPRLPQQAVLFRERYVWDKQQRA
                     AIAAYDETIKNFQQEQHIPVKAWSEQALNRIKDAPGLHGRDRLRDALAALGFGLK"
     gene            complement(961..1920)
                     /locus_tag="VGB93_04140"
     CDS             complement(961..1920)
                     /locus_tag="VGB93_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0145339.1"
                     /translation="MTERYVGASGLKVSLAGLGANNFGDRVSNDVAKEIIHAAIDNGI
                     TFIDTADVYGRGGSESILGDALGTHRKDVILATKFGRSMDPAKHKGSASRRYIIQSVE
                     ASLTRLKTEWIDLYQYHFPDPDTPIDETLRALDDLVHTGKVRYIGLSNFPAWQVVDAQ
                     WKTKVAGLEPIISSQNNYSLLDRSVEKELLPALRANGIGLLPYLPLAGGFLTGKYKRN
                     VPLPEGSRFARLQRNATRFLKDGNFDILDRLTSFAAARGKTVADLALAWLAAQPSVAS
                     VIAGASNREQVIANVRSLDWKLSPEDLAELDQIGISNSFEKAA"
BASE COUNT          523 a          683 c          652 g          468 t
ORIGIN      
        1 agacaagcgc caagcgcata ctgaatctcc gagcggcgat ggcggcacac gctcacttca
       61 acccgaagcc caaagccgcc agagcatctc gcaggcggtc gcggccatgc agccccggcg
      121 catctttgat gcggttgagc gcttgctcgc tccaagcctt caccgggatg tgctgctctt
      181 gctgaaaatt tttgatggtt tcatcgtagg ctgcgatggc ggcacgctgc tgcttgtccc
      241 aaacgtatcg ctcacgaaac agcaccgcct gctgcggcag ccgcggtttt acgccagtcg
      301 gcacaccggg atccggatga ccaacaacaa gtccaaaaag cgggaaaacg tgggcaggca
      361 atccaagctc tttggccact tcggccggtt tattacggat ggcgccgatg tagacagtcc
      421 cgaggccgag agactctgcc gcgacgacag cgttctgcgc ggcgagcgag gtatcgacag
      481 caccgactaa aaaactctcg atgtaatcga gtccctcggc caagaggccg cgttctttgg
      541 cgagttcgtt gaggcgggca agatcgagca gccaaacgag gaacaacggc gcgcgaataa
      601 tgtgcttttg atcgttagcc aacgcggcga gccgcgcctt gcgatcctta tcctcaaccc
      661 cgacgacgct ccaagcctga agattggagg aggaagacgc ggactgcgcc gccgtcacca
      721 acacctcgag tgttccttcc ggcaaacgat ctggaagata ggctcgcacc gagcggtgcg
      781 aaaataaaag atcgagcgtc tcgttgcctc gctcggggaa ggcgaaattt tccaggccgt
      841 aacgttgccc ggcacgattt cgagtcgcct ccaacgcgtt tcgatctcct ggttcgcgtc
      901 tcgctgatga aacactcatg ctctgtcctt gctcgtagtt ctgattacgg ggcgcgtggg
      961 ctacgctgct ttttcgaagc tgttggaaat gcctatttga tcaagctccg cgagatcctc
     1021 gggtgacaac ttccaatcga gcgatctcac attggcgatc acttgttcac ggtttgaggc
     1081 gccggcgatg acgctcgcga cgctcggctg agcggccaac caggccaggg caagatcagc
     1141 gacagtcttg cctcgtgccg ccgcgaaact cgtgaggcga tcgagaatgt cgaaattgcc
     1201 gtccttgaga aagcgtgtcg cattgcgctg aaggcgggca aagcgcgagc cttctggcaa
     1261 ggggacattg cgcttatatt tgccggtcaa aaagccgcct gctaggggca gatacggcaa
     1321 gaggccgata ccgttagccc gcagcgccgg caaaagctct ttctcgacgg aacgatcaag
     1381 aagactgtaa ttgttttgtg acgaaatgat cggctctagg ccggcgacct tggtcttcca
     1441 ctgcgcgtcg accacttgcc aggccggaaa gttcgaaaga ccgatatagc ggaccttacc
     1501 ggtgtgaacg agatcgtcga gggcgcgcaa tgtctcgtcg atcggcgtgt cggggtccgg
     1561 aaaatgatat tgatagagat cgatccactc cgtcttcaaa cgcgtcagac tcgcctcgac
     1621 agactggata atatatcggc gcgacgcgct gcccttatgt ttggcaggat ccatcgagcg
     1681 tccgaatttg gtcgcaagga tcacatcttt ccgatgcgtg cctagcgcat cgcccagaat
     1741 cgattccgag ccaccccgac catatacgtc ggccgtgtcg atgaaagtta tgccgttgtc
     1801 gatcgctgca tggataattt ctttcgcgac atcgttcgac accctgtcac caaaattatt
     1861 ggcgccaagg cccgccaagg agactttaag ccccgacgcg ccaacataac gctctgtcat
     1921 ccgtgccccc ttcgcctccc gaaggtccag ctgctaagag ccgtaaccat cgaagaagct
     1981 aaatgctatc aaatttatag ggaatagcaa atgacctcgg cccatggcct ggcaccattt
     2041 gcaatgggcc ggaagcggaa tagcggggct ttgagcgatt tgcacgtgaa gcggcgaccg
     2101 ccccccatac gttcgacaag aagcttccgc tcacaagaag caatcagatc gtccctcact
     2161 cactaaggcg cctcgttcca tcggccgccc cggagaaacg tcagccagcc gagcgccgca
     2221 cctgtgtgtc gcatcacttg gtacgtaaaa ggctcaataa aagaggccag aatgctgacc
     2281 gttaccctgg ctgagctcgt gggatcgacc catcggcgat aaagat
//
LOCUS       DASWOG010000205         6612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_4813, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000205 DASWOG010000000
VERSION     DASWOG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4813"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VGB93_04145"
     CDS             <1..549
                     /locus_tag="VGB93_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY0145340.1"
                     /translation="WPCNGEAPLGTPTMHIDHFVRGKGQFLLTEYIPTDEKTGPRFPL
                     MLTTGRILSQYNVGAQTRRTANSTWHPEDVLEIHPHDAEDRGIRDGDWVKIKSRAGET
                     ALRAKITERMSPGVVYTTFHHPLSQANVVTTDYSDWATNCPEYKVTAVQISTSNGPTE
                     WQEEYREMSEQNSRIAPLDAAE"
     gene            546..1406
                     /gene="fdhD"
                     /locus_tag="VGB93_04150"
     CDS             546..1406
                     /gene="fdhD"
                     /locus_tag="VGB93_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY0145341.1"
                     /translation="MSDTEDETSESLPAPAVSVSCAAQRGGVSFPTTRIVPDEQAVAF
                     TYNGSTHAVMMATPADLEDFSIGFALTEGLIEDRSEIEDLEIMPTPLGIEARVWLKGD
                     RAEAYAKRRRSMAGPTGCGLCGIESLEAAMRPLPRVTAEAKVSPQAIIAAMASLDPAQ
                     TLNSVTHAVHGAGFWTEREGLVALREDVGRHNALDKLVGALVRDGRTAADGMILMTSR
                     VSIELIQKAARINAPVLVAVSAPTAAAIKLADEAGMTLVAVARRQDFEVFTHPERLNE
                     TSRQVASHVA"
     gene            1396..1662
                     /locus_tag="VGB93_04155"
     CDS             1396..1662
                     /locus_tag="VGB93_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEY0145342.1"
                     /translation="MSHETHEKSHTLDRLIYMANQIGGFFATQGHDKEVAGVADHVEK
                     FWNSRMLKQIYAHIASGGEGLAPPVKEALQMLERKQQAQAAQQQ"
     gene            complement(1664..2560)
                     /locus_tag="VGB93_04160"
     CDS             complement(1664..2560)
                     /locus_tag="VGB93_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0145343.1"
                     /translation="MNVPNPDFGTPRVDYTPPHIVQRRIAAWRGLWAETIELTQSEPY
                     EYDFQAPFHLLIAAERSERYAGETCLEGLPNSYQRDLSRRMTFVPAGRRFCGWQKPRV
                     LSRVNLFYIDPRGPFLDPELGFDKIELKPQLFFFDPDMWVTALKLKAQITAPNAVSSP
                     YAEALGAVLCHELIRLNGGAKAPPRSGRQTGLAPWQQKRVANYIETRLDARLTIADMA
                     ALVHLSPFHFSRAFKQSFGMPPHRYHMMRRVEAAKTLLNATSTPMTEIALQLGFSEAS
                     SLSAGFRRVTGVSPSAYRRIAG"
     gene            2860..6039
                     /locus_tag="VGB93_04165"
     CDS             2860..6039
                     /locus_tag="VGB93_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0145344.1"
                     /translation="MGIVRFALRFPHTFYVMALAILFLGISAIISAPKDIFPVINIPV
                     VTVIWQYTGLTPQEMEARVTTYSEYSISANVNNVRDMESQTLSGVAVQKIFFQQGVSV
                     DLAIAQIVSATNAIRALMPPGIQPPIIVQYNASSVPVLQLSLSSDHMNEQQLYDYGIY
                     SLRQALAPIQGITLPTPYGGKYRQIMVDLNPDALRANGLTPNDVLTAVNAQSLTLPSG
                     DAKIGNTQYIVSMNAMAETIDALNRLPLRQIGATTIRLADVAHVRDGWAVQQNVVRAN
                     GKRSVLLTIIKNGDASTLDVVNAVKAQLPELLKAAPPGMEMHVLFDQSVFVSEAINSV
                     LREGAIAAGLTALMILIFLGSWRSTLIVMVSIPLSILSSIAIFSALGQTINTMTLGGL
                     ALAVGILVDDSTVAIENTHHLFEENMEFDDAVLNGSAGIALPTLVSTLAICSVFVSVF
                     FLQGAARYLFTPLGMAVAFAMLTSYILSRTLTPIIIRILLRKEQAQHAAGHSSWFSRF
                     HDGFNAGFDRFREGYTYVLEHVLASRIVVPVVALCVIGGAAFLATQVGTDFFPQVDTG
                     LIQLHVRAPARTRIEATEGIFQAIEDRIRQDIPAKNLDLILDNIGLPQRTYNLAFTDG
                     SAIGVNDGTIQIALKEGHPPTFEIVKKLRRDLTREFPNVLFYSQPADMVTQILNFGIP
                     SQIDVVVQGPDRAGNQDVAKELRERMSRVVGLVDVHIQQELDAPELYYFVDRIRAQEL
                     GLNINSIANNLNISLSSSEQVSPNFWTDPKNGIPYYFAVQTPEYRIASLNDLNNTPVV
                     SQASTDTPVPNILGNVASMHRQYIQSVYNHANIQPLYDIYASVQDSDLGSVAKNISSI
                     VKDLQPKLKPGNKFIVRGQIDSMNSAFQDLTFGLLFAAVFVYLLMVVNYQSFLDPLAI
                     ILALPGAGAGILLMLYVTGTSISVPSLMGAIMSIGVASANSILLVTFARDQRHDGLNA
                     LDAARSAGRTRIRPVLMTAAAMMVGMIPMAIGAPGEEQNAVLARAVIGGVLIGTVTTL
                     LFVPWLYSIIGRFEPTENPQHEADSEPAR"
     gene            6083..>6612
                     /locus_tag="VGB93_04170"
     CDS             6083..>6612
                     /locus_tag="VGB93_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0145345.1"
                     /translation="MRDEVQQPQKPGPEPAQKPKETQPHAERPRHLGLVAICALLALA
                     GLIAFGVWGHAQRSTAAADTQKQQEEAVPEVRVARVGSTDAPRVLDLPGSTEAFDAAT
                     LYARATGFISKRNVDIGSEVKAGDVLAIIAAPDLDQQLLQAEAQVAQLQAAIAQAQAN
                     LQLAQVTNTRTQTLVG"
BASE COUNT         1343 a         2123 c         1910 g         1236 t
ORIGIN      
        1 tggccttgca atggggaggc gccacttggc acgccgacca tgcacatcga tcatttcgtc
       61 cgcggcaagg gccagttcct gctgaccgaa tatattccga ccgacgagaa gaccgggcca
      121 cgcttcccgc tgatgctgac gacgggccgc attctgtcgc aatacaatgt cggcgcgcag
      181 acccggcgta cggccaattc gacctggcat ccggaggacg tgctcgaaat ccatccgcat
      241 gacgccgaag accgcggcat tcgcgatggc gattgggtga agatcaagag ccgcgccggc
      301 gagacagcgc ttcgcgccaa gatcaccgag cgcatgtcgc caggggttgt gtacacaacc
      361 ttccaccatc cgctgagcca ggcgaatgtc gtcacgaccg attattcgga ttgggcgacc
      421 aattgtcctg aatacaaggt cacggccgtt cagatctcga cgtccaacgg cccgaccgaa
      481 tggcaggagg aatatcgcga gatgtccgag caaaatagcc ggattgcacc tctcgacgcg
      541 gccgaatgag cgacacagaa gacgaaactt cagaatcgct gccagccccc gcggtcagcg
      601 tttcctgcgc ggcgcagcgc ggcggcgttt ctttcccgac gacgcggatc gtccctgacg
      661 agcaagccgt cgccttcacc tacaatggct caacccatgc ggtgatgatg gcgacgcccg
      721 ccgatcttga ggacttttcg atcggcttcg cgctgaccga agggctgatc gaagatcgca
      781 gcgagatcga agatctggaa atcatgccca cgccgctcgg cattgaagcg cgtgtctggc
      841 tcaagggcga ccgcgccgaa gcctatgcca agcgccggcg tagcatggcg ggtccaacgg
      901 ggtgcggcct ctgcggcatc gaaagccttg aggcggcgat gcgaccgctg ccgcgcgtga
      961 ccgccgaggc aaaagtctcg cctcaggcaa ttatcgccgc gatggcctcg ctcgatcccg
     1021 cacagacctt gaacagcgtg acccacgccg tccacggtgc cggattctgg accgaacgtg
     1081 aaggccttgt cgccctccgc gaggatgtcg gtcgccataa cgcgctcgac aagctcgtcg
     1141 gcgccctcgt ccgcgatggc cgcacggcgg cggatgggat gatcctcatg acgagccgtg
     1201 tttcgattga actgatccag aaggcggcga ggatcaacgc cccggttttg gtcgccgtgt
     1261 cggcgccaac agccgccgcc ataaagctcg ccgacgaggc aggaatgacg cttgtcgcgg
     1321 tcgcgcggcg tcaggatttc gaagtcttca cccatcccga gagattgaac gaaacctccc
     1381 gccaagttgc atcacatgtc gcatgagacg catgagaagt cgcatacgct cgaccggctg
     1441 atctacatgg ccaaccagat cggcggcttt ttcgccacgc agggtcacga caaggaagtt
     1501 gccggcgtcg ccgaccatgt cgagaaattc tggaattcga ggatgctcaa gcagatctac
     1561 gcgcatatcg ccagcggcgg cgaaggctta gcgccgcctg tcaaagaagc actgcaaatg
     1621 cttgagcgca aacagcaagc tcaggccgcg cagcagcagt aattcagcca gcaatccggc
     1681 gatacgcact cgggctcacg cccgtcaccc gacgaaaacc cgcgctcagc gaactcgcct
     1741 cgctgaagcc gagttgcagc gcgatctcgg tcatcggcgt cgatgtcgcg ttgagcagcg
     1801 tcttcgccgc ctcgacacgc cgcatcatgt gatagcggtg cggcggcatg ccgaaggatt
     1861 gtttgaacgc ccgggaaaaa tgaaacgggc tcagatgcac cagcgccgcc atgtccgcga
     1921 tcgtcagacg agcgtcgagc cgggtctcga tataattcgc gacgcgcttc tgctgccacg
     1981 gcgcaagccc agtctggcgg ccgcttctcg gcggcgcttt ggccccgcca ttcaatcgga
     2041 tcaattcgtg gcaaaggacc gcgccaagcg cctccgcgta ggggcttgag accgcattcg
     2101 gtgccgtgat ctgcgccttg agcttaagcg cggtaaccca catgtcgggg tcgaaaaaga
     2161 aaagctgcgg cttcagctcg attttatcga agccgagttc gggatcgagg aagggcccac
     2221 gcggatcgat gtaaaagagg ttcacgcggc tcaaaacgcg gggcttctgc caaccgcaga
     2281 agcgacgccc ggcagggacg aaggtcatcc ggcgcgacaa gtcgcgctga taggagtttg
     2341 gcagcccctc aaggcacgtc tcgcccgcgt aacgctcgga gcgctcggcg gcaataagaa
     2401 gatgaaaggg cgcttgaaaa tcatattcgt acggctcgga ttgggtcagt tcgattgttt
     2461 ctgcccaaag cccacgccag gcggcaatac gccgctgaac gatgtgaggg ggagtgtaat
     2521 caacccgcgg agtgccgaag tcgggattcg gaacgttcat tgaccatcca aaaagtgaag
     2581 gacttcatcc cctaacgtcc ctcgcccaag cttgattttg gttcggatat ggccgcgcca
     2641 ccggccgact tcaacgccat tcacgcgcca gtgagcgtac ttagcccgat ttccttgaat
     2701 ctaaggtgaa ttccgctccg atgagcaaga ccgatcaagc ggagccaaac cggcgttgat
     2761 agattagggt aggctgaccg cttcggaacg gcctgcgaag ctttgcgcgt cctgcgccgc
     2821 gaagtcttct cccagataat cgatcggtcg gagccgggaa tgggtatcgt tcgcttcgcg
     2881 ttacggtttc cacacacgtt ttacgtgatg gcgctggcga ttctgttcct cggcatcagc
     2941 gcgatcatct ccgcgcccaa agacattttt ccggtcatca atatcccggt cgtcaccgtc
     3001 atctggcagt ataccggcct gacgccgcag gagatggaag cccgcgtcac gacctacagc
     3061 gaatattcga tcagcgcgaa cgtcaacaac gtccgcgata tggaatcgca aacgctgtca
     3121 ggcgtcgccg tccagaagat tttcttccag cagggcgtga gcgtcgatct cgccattgcg
     3181 cagatcgtct ctgccaccaa cgcgatccgc gccctgatgc cgcctggcat tcagccgccg
     3241 atcatcgtgc aatataacgc atcgagcgtt ccggtccttc agttgagctt gagctcggac
     3301 cacatgaacg agcagcagct ttacgactac ggcatctaca gcctgcgtca ggcactggcg
     3361 ccgatccagg gcatcaccct gccgacgccc tatggcggca aataccgcca gatcatggtc
     3421 gatctcaacc cggacgcgct gcgcgccaac ggattgacgc ccaacgatgt cctcaccgcc
     3481 gtcaatgcgc aaagcctcac cctgccctcc ggcgacgcca agatcggcaa tacgcaatac
     3541 atcgtcagca tgaacgcgat ggccgaaacc atcgacgctc tcaacaggct gcccttgcgc
     3601 cagatcggcg cgaccacaat acgcctcgcc gacgtggcac atgtccgcga cggctgggcc
     3661 gtccagcaaa atgtcgttcg cgccaatggc aagcgctccg ttcttctgac catcatcaag
     3721 aacggcgacg cgtcgacgct cgatgtcgtc aatgcggtca aggcgcaatt gccggagctg
     3781 ctcaaggcgg cgccgcctgg catggagatg cacgtcctct tcgatcaatc cgtcttcgtc
     3841 agcgaggcca tcaacagcgt gcttcgcgaa ggcgccatcg ccgcgggcct caccgccctg
     3901 atgatcctca tcttcctcgg ctcgtggcgc tcgacgttga tcgtgatggt ttcgatcccg
     3961 ctctcgatcc tgtcctcgat cgcgatcttc tcggcgctcg gtcagacgat caatacgatg
     4021 acgctcggcg ggctcgccct ggcggtcggc attctggtcg atgattcgac agtcgcgatc
     4081 gaaaacacgc atcacctgtt cgaagagaac atggaattcg acgacgcggt gctgaacggc
     4141 tcagcgggga tcgccttacc gacgctggtg tcgacgctcg cgatctgtag cgtcttcgtg
     4201 tcggtcttct tcctgcaagg cgccgcgcgc tatctgttca cgccgcttgg catggcggtc
     4261 gccttcgcga tgctgacctc ctacatcctg tcacgcactc tgacgccaat catcatccgc
     4321 atcttactgc gcaaggaaca ggcgcagcac gccgctgggc attcctcctg gttcagccgc
     4381 ttccacgacg ggttcaacgc cggtttcgat cgtttccgcg agggctacac ctacgtgctg
     4441 gagcacgtgc tggcgagccg catcgtcgtg ccggtcgtcg cgctgtgcgt cattggtggc
     4501 gcggcctttc tcgcgacgca agtcggcaca gacttcttcc cgcaggtcga taccggcttg
     4561 atccagcttc atgtccgcgc cccggcgcgc acgcgcatcg aagcgacgga gggaatattc
     4621 caggcgatcg aagaccgcat ccgccaggat attccggcca aaaatctgga tttgatcctc
     4681 gacaatatcg gcctgccgca gcggacctat aatctcgcct tcaccgacgg ctcggcgatc
     4741 ggcgtcaacg acggcaccat ccagatcgcc ttgaaagagg gccatccgcc cacgttcgag
     4801 atcgtcaaaa agctgcgtcg ggatctcacc cgcgaatttc ctaatgtgct gttctattcg
     4861 cagccggcgg atatggtgac gcagatcctc aacttcggca ttccctcgca gatcgatgtc
     4921 gtcgtgcaag ggcccgaccg cgccggcaat caggatgtcg ccaaggaact acgcgagcgc
     4981 atgtcacgcg tcgtcggcct cgtcgatgtc catattcagc aggaactcga tgcgccagaa
     5041 ctctattact tcgtcgaccg catccgtgcg caggaactcg gcctcaacat caacagcatc
     5101 gcgaacaatc tcaacatcag cctcagttcg tcggaacagg tgtcgcccaa cttctggacc
     5161 gacccgaaga acggcattcc ctattacttc gccgtgcaga cgccggagta ccgtatcgcc
     5221 agcctcaacg atctcaacaa cacgccggtt gtgagtcagg cttccaccga tacgccggtg
     5281 ccgaacattt tgggcaacgt cgcctcgatg caccggcaat atatccagtc cgtgtacaat
     5341 cacgccaaca tccagccgct ctacgacatt tacgcaagcg tgcaggacag cgatctcggc
     5401 agcgtggcca agaacatcag cagcatcgtg aaagaccttc agccaaaatt gaaacccggc
     5461 aacaagttca tcgttcgcgg ccagatcgac agcatgaact cggcctttca agacttgacg
     5521 ttcggactgc tgttcgccgc cgtcttcgtc tatcttctga tggtcgtgaa ctaccagagc
     5581 ttcctcgatc ctctggcgat catcctggcg ctgccgggtg ccggcgcggg cattttgctg
     5641 atgctctatg tgaccgggac gtcgatcagc gtgccgtcgc tgatgggcgc gatcatgtct
     5701 atcggtgtcg cctcggccaa ttcgatcctg ctcgtgacct tcgcgcgcga ccagcggcat
     5761 gacggtctga acgctctcga tgccgcacgc tccgccgggc gcacccgtat ccggccggtg
     5821 ctgatgacgg cggcggcgat gatggtcggc atgataccca tggcgatcgg cgcgccgggt
     5881 gaggagcaga acgcagttct ggcgcgcgcg gtgatcggcg gcgtcctcat cggcacggtg
     5941 acgacgcttt tgttcgtgcc ctggctctat tctattattg gccggtttga gcccaccgag
     6001 aatccgcagc atgaggcgga cagcgagccg gcccgatgag caagttcgct tcaatagttt
     6061 tcgcagataa ggattttacc tgatgcgcga cgaagtccag cagccccaaa agcccgggcc
     6121 ggaaccggcg cagaaaccaa aagaaacgca gccgcacgcg gagcggccgc gccacctcgg
     6181 tcttgtcgcc atttgcgcac ttctcgccct cgccggcctt atcgcctttg gcgtttgggg
     6241 ccatgcgcag cgcagcacag ccgccgccga tacccaaaag cagcaggaag aggcagtgcc
     6301 ggaggtccgc gtcgcgcgtg tcggcagcac ggacgcgcca cgcgtgctcg acctcccggg
     6361 tagcaccgag gccttcgacg cggcaaccct ctatgcacgc gcaactggct ttatctcgaa
     6421 gcggaacgtc gatatcggca gcgaagtgaa ggcgggcgac gtcctcgcga tcattgcggc
     6481 gcccgatctc gaccagcagc ttttacaagc cgaagcgcaa gtcgctcagc ttcaggcagc
     6541 catcgctcag gcacaggcga acttgcaact cgcgcaggtc acgaacacga gaacccagac
     6601 ccttgtcggc ca
//
LOCUS       DASWOG010000206         6281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_206123,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000206 DASWOG010000000
VERSION     DASWOG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206123"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VGB93_04175"
     CDS             complement(<1..870)
                     /locus_tag="VGB93_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0145346.1"
                     /translation="MDQLQITAGFATRVGTRPDNQDFGLIDIGTASQRATQGSIAALA
                     DGFGAKSGRVASELAVRAFVDGYRSQREPVGVATAAMKSLNAYNLWLHGQSRSDPALL
                     GAAATFTAVVLLGRIATILHVGDSRAWHLRGDHLTLLTDDHSAPQADGNRRLLRAIGL
                     EPALRLDVKAQPLEVHDRLLLTTKGIHSVLSANALLRLLSARQTSQADANAVVEAALQ
                     AGSEDNATALVIDIITVPAPNYSAVVAEMENLPLKNLPAIGDTIDGFKLARVLTESKT
                     SRLFLARDYNEWVV"
     gene            complement(1036..1111)
                     /locus_tag="VGB93_04180"
     tRNA            complement(1036..1111)
                     /locus_tag="VGB93_04180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1076..1078),aa:Ala,seq:cgc)
     gene            1224..1514
                     /locus_tag="VGB93_04185"
     CDS             1224..1514
                     /locus_tag="VGB93_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0145347.1"
                     /translation="MRAVLDVLLLVLQIYTYIIIIVAIMSWLIAFNVINIHNNVVRSI
                     WNALNAVTEPLLKPIRSILPAFGGLDISPVILLLLIFLLQDIIERYVYPNVF"
     gene            1529..1840
                     /locus_tag="VGB93_04190"
     CDS             1529..1840
                     /locus_tag="VGB93_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEY0145348.1"
                     /translation="MSQPWVVREDGILVIVRLTPKSSRDEIAGVEHLSDGRAVLKARV
                     RAVPEAGAANEALIRLLAKSLSLAPSHVRLESGQTSRVKTIRVAGDSAALAAKLAALT
                     A"
     gene            complement(1837..2508)
                     /locus_tag="VGB93_04195"
     CDS             complement(1837..2508)
                     /locus_tag="VGB93_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HEY0145349.1"
                     /translation="MILSAAFAALAQIFTPPFRRVFWKTLGLTLLLLVLVFAGAETAL
                     AHFLVLPYHWLATSLTLLAAVALLVGFAFAITPVSFLVAGFFFDELAGIVEADIDPQH
                     PGRTAPLGEQIRVAIVFALLALALNIVALALLLVPGVNAVIFLLVNAYLLGRGYFELA
                     ALRYRSVEDVGRVRRAHEFQIFLAGMFIAALAAVPIANLLTPLFGAALMVRVHDKIAI
                     QAAKA"
     gene            2640..3644
                     /gene="bioB"
                     /locus_tag="VGB93_04200"
     CDS             2640..3644
                     /gene="bioB"
                     /locus_tag="VGB93_04200"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY0145350.1"
                     /translation="MNISSPRDIRYDWTADEIVAVHDRPLLELIGEANRLHRVYQPAN
                     YVQKASLLSVKTGGCPEDCAYCPQSAHHEVNLGKSSLMPVSDVLDAAKKARNAGADRF
                     CMGAAWRQVRDGAEFDAVLDMVRGVRALGMEACVTLGMLTESQAQRLKEAGLTAYNHN
                     LDTGPDYYAQIISTRTYDDRLATLRAVRAAGIEMCCGGIVGMGESVHDRAAMLAVLAA
                     FDPHPESVPINALVAVDGTPLAGREKIDPLVFVRMVATARIVLPRSIVRLSAGRASFS
                     REAQVLCMIAGANSIFYGDTLLTTPNADVPEDNALFAALASIQRDAAPAEDTAALSPA
                     "
     gene            3734..3949
                     /locus_tag="VGB93_04205"
     CDS             3734..3949
                     /locus_tag="VGB93_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0145351.1"
                     /translation="MSSTTDKIKGVANEAACSVKQGVGKAVGSDKLRAEGAAQEVKGH
                     AQKAVGDAKDAVKTAVNKSAEHINKNI"
     gene            complement(4013..4423)
                     /locus_tag="VGB93_04210"
     CDS             complement(4013..4423)
                     /locus_tag="VGB93_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0145352.1"
                     /translation="MEHGWAKLARVPENFTSILAALHMPFPGLLAWLTIFTELFGGLA
                     VLAGALVPLASVPMAIVLLVAIFTVHLPNGFSSIKLEAVSPRGAHFGQPGYETDLLYL
                     AGLAALTLGGTGRWSLDNVLVRYLGALASSRNKI"
     gene            complement(4614..5585)
                     /locus_tag="VGB93_04215"
     CDS             complement(4614..5585)
                     /locus_tag="VGB93_04215"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY0145353.1"
                     /translation="MKRSLRIALYLCAWTLLRHDASACSLVKVAELPLIELGNHYAVM
                     ANVAGQSRPMLVDTGAATTLLGAKAVDDLNLKVDYSAPLPKPVLGIGQTSAAVVYLNV
                     IAPVLGFGDLIFHNRSTIVASLDFGPMPEADAVGILGDDILSDYDVEFDFLDDRLTLY
                     DEFACSDAFVPWTGAYFALPFEHRDTKIMVDIFLNGERTPAIVDTGNNVSFVSRMAPA
                     LWGALAHEMKKTHLHSTSPFNGGTSLPNQRYQFDEIRIGEDVYQNEYMKVIQVQLPGA
                     SANLGLDYWKQRKLWISYPNNLIFVSGMNTSPALAYPIKANTSIAGK"
     gene            complement(5691..5766)
                     /locus_tag="VGB93_04220"
     tRNA            complement(5691..5766)
                     /locus_tag="VGB93_04220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5731..5733),aa:Ala,seq:ggc)
     gene            complement(5919..6050)
                     /locus_tag="VGB93_04225"
     CDS             complement(5919..6050)
                     /locus_tag="VGB93_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145354.1"
                     /translation="MEDDVELEDEGGDDDDTFLEEEEEDSDDVTGLIDGDLAPDEEP"
     gene            complement(6123..6281)
                     /locus_tag="VGB93_04230"
     CDS             complement(6123..6281)
                     /locus_tag="VGB93_04230"
                     /inference="COORDINATES: protein motif:HMM:NF021083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEY0145355.1"
                     /translation="MAKPDLGNKHTCQNCGTKFFDLYKSPVVCPKCGTVHQATTRTAR
                     AIVVEKDE"
BASE COUNT         1299 a         1835 c         1881 g         1266 t
ORIGIN      
        1 caccacccat tcattgtaat cgcgtgccag aaagaggcgt gacgtcttgc tctcggttag
       61 aacgcgggca agcttgaatc cgtcaatagt gtcgccaatg gccggcaagt tcttgagcgg
      121 caaattctcc atctcggcga cgacagcgct atagttaggg gcgggcaccg tgatgatgtc
      181 gatgacgagt gctgtcgcgt tatcttcgct tccagcttgc agtgcagcct cgacaaccgc
      241 attggcgtcg gcctgcgatg tttgtcgcgc gctgagaagt cttaaaagcg cgtttgccga
      301 aaggacggaa tgaatgcctt tcgtcgtgag caagaggcga tcatgcacct caagcggctg
      361 cgctttcaca tccagccgga gcgccggctc gaggccgatc gcgcgcagca gtctccggtt
      421 gccatccgct tgcggggcgg aatgatcgtc tgtcagtagc gtcagatggt ctccgcgcag
      481 atgccacgcc cggctatcgc cgacatgcag gatcgttgcg attcggccca atagaaccac
      541 ggcggtaaag gtcgccgcag ccccgagcag cgccgggtcg gatcgcgact ggccatgaag
      601 ccaaagattg taggcgttga gcgatttcat cgcagcagtg gcgacgccga caggctctct
      661 ttgtgaccga taaccatcga caaaagcgcg gacggcaagt tccgacgcca cccggccgct
      721 ttttgcgcca aagccatccg ctagcgctgc gatcgagcct tgcgtggctc tctgggaggc
      781 ggtgccgata tcgataagac cgaagtcttg attatccggc cgcgtcccga ctcgggtggc
      841 aaatcctgct gtgatctgaa gttgatccaa gaacgtaacc cgagggcacc atcagacttg
      901 ttcaaagcat atggcgcgcc acatcgcccc aggacattcg tatcgtcgtc tggcagtaag
      961 agcactcgcg gcacggccga tcggtgacgg gccacgcaaa ccgcgtgaca atgtagcgta
     1021 tgtatttgat tctattggtg gagctgatga gattcgaact cacgacctct gcagtgcgat
     1081 tgcagcgctc tcccatctga gctacagccc cggccagcgg ggtgtttacg tctcctgcgg
     1141 cgcaactgtc aatgtaggct gctgcttgcc gggccgcctt tcgccagcta aacgggaggc
     1201 ccttgccggg ttccggagcg gtcatgcgtg cagttctcga cgttcttttg ctcgttttgc
     1261 aaatttacac ctacatcatc atcatcgtgg cgatcatgtc ctggctgatc gcctttaacg
     1321 tgatcaatat ccataacaac gtcgtccgct cgatctggaa cgcgctcaac gctgtcaccg
     1381 agccgctgct gaaaccgatc cgcagcatcc tgcccgcgtt tggcggcctc gatatttcgc
     1441 ctgtgatcct gctcttgctg atctttctcc ttcaggacat catcgagcgc tacgtctatc
     1501 cgaacgtctt ctagtccgac atccggctgt gtcgcagcct tgggtcgtgc gtgaggacgg
     1561 cattctcgtc atcgtgcggc tcacgcccaa atcgtcacgc gacgagatcg ccggggtcga
     1621 gcacttgtcg gacggccgcg ccgtcttgaa agcgcgcgtc cgtgcggtgc cggaggcggg
     1681 ggcggcgaat gaggcgctga ttcgcctgct ggccaaatcc ctatcgctcg cgcccagtca
     1741 tgtgcgcctc gaatccggcc agacgagccg ggtgaaaacg atccgtgttg ccggcgattc
     1801 cgccgcgctt gccgcgaaat tggcggcgct gacggcctag gctttggccg cttgtatcgc
     1861 aattttatcg tggacgcgca ccatcagcgc ggcgccgaaa agcggcgtca gcaggttggc
     1921 gatcggcacc gccgccagcg ctgcgataaa cattcccgcg agaaaaatct gaaattcgtg
     1981 cgcccggcgc acgcggccga cgtcctcaac ggaccggtag cgtagcgccg caagctcgaa
     2041 atagccccgg ccgagcagat aggcgttgac gagcaggaag atcaccgcat tgacgcctgg
     2101 caccagcagg agcgccagcg ccacgatatt gagggcgagc gccagcaaag caaacacgat
     2161 agcgacccgg atttgctcgc caaggggcgc cgtgcggccc ggatgctgcg ggtcgatgtc
     2221 cgcttcaacg atgccggcaa gctcatcgaa gaagaagccg gcgacgagaa acgacactgg
     2281 cgtgatcgcg aaggcgaagc cgacgagcag cgccacggcg gcaaggagcg tcagacttgt
     2341 cgcaagccag tgatagggga gcacgaggaa atgcgccaac gccgtctcgg caccggcaaa
     2401 gaccagcaca agcagcagca gggtgaggcc taaagtcttc cagaagacgc ggcggaacgg
     2461 cggcgtgaaa atctgggcga gggcggcgaa ggcggcggag aggatcatct gggatcgttg
     2521 gctttggcgg tcgagggttg gtcaggctga tcactaccta tgcgaccgat catgccctgg
     2581 cgagggcaga gccggcccga tccttgtctt ggcggtaatt tccttttaaa tcgagggcga
     2641 tgaacatttc ttcgccgcgg gatattcgct acgattggac ggctgacgaa atcgtcgccg
     2701 tgcatgatcg tcccctcctc gaattgatcg gcgaggccaa ccggctgcat cgcgtctatc
     2761 agcccgcgaa ttacgtccag aaggcgagcc tgctcagcgt caagaccggc gggtgcccgg
     2821 aagattgcgc ctattgcccg caatcggcgc atcatgaggt caatctcggc aaatcatcgt
     2881 tgatgccggt gagcgacgtg ctcgatgcgg caaaaaaagc gcgcaatgcc ggtgccgacc
     2941 gtttctgcat gggcgccgcc tggcggcagg tgcgcgacgg cgccgaattc gatgcggtgc
     3001 tagacatggt gcgcggcgtc cgtgcgctcg gcatggaggc gtgcgtcacg cttgggatgc
     3061 tgaccgaatc gcaggcgcag cggctcaagg aggcgggcct taccgcctat aaccacaacc
     3121 tcgacaccgg cccggattat tacgcgcaga tcatttcgac gcggacctat gatgatcggc
     3181 tcgcgacctt gcgggctgtg cgcgccgccg gcattgaaat gtgctgcggc ggcatcgtgg
     3241 gcatgggaga aagcgtgcat gaccgtgctg ccatgctggc ggttctggcg gccttcgatc
     3301 cgcatcctga aagcgtcccg atcaatgcgc ttgtcgcggt ggatgggacg ccgcttgctg
     3361 gtcgcgagaa gatcgatccc ttggttttcg tgcgcatggt tgcaacggcg cggattgtgt
     3421 tgccgcgcag cattgtgcga ctctcggctg gccgcgcgag cttcagccgc gaagcccaag
     3481 ttctttgcat gattgctggc gccaattcga ttttctatgg cgataccctt ttgacgacgc
     3541 cgaatgccga tgtgccggaa gataatgcgc tcttcgctgc actggcgtcg attcagcgcg
     3601 acgcggcccc agccgaagat acggcggcgc tttcgccagc gtgaattatt tgtaatccca
     3661 gcgaaattca tatccacgga actattctta tcctgtgccg ttcctcgctc cgaagttacc
     3721 aaggagtgag gacatgagca gcacgactga caagatcaaa ggtgtcgcga acgaggccgc
     3781 ttgttccgtc aagcagggcg tcggcaaggc tgttggctcc gacaagcttc gtgccgaagg
     3841 cgcggcgcag gaggtgaagg gtcatgcgca gaaggccgtc ggcgatgcca aggacgcggt
     3901 gaagaccgcc gtcaacaagt cggccgagca cattaataag aacatttgat cgtcgactta
     3961 agtcgatgag aaaagggcgc cctacctacg ggcgcccttt ttatttgggg cgctaaatct
     4021 tattgcgaga cgatgccagc gcgccaaggt agcgcaccaa gacgttgtcc aaggaccagc
     4081 gtcctgtgcc gccgagagtc aatgccgcga gacccgcaag gtagagtaag tctgtttcat
     4141 agcccggctg gccaaaatgc gcgccacgag gtgacacggc ctcaagcttt atcgaactga
     4201 atccattcgg cagatgcact gtgaatatcg ctaccaaaag cacgatggcc atgggtacgc
     4261 tcgccaacgg aaccagcgcg cccgccaaca ccgcgaggcc gccgaatagt tccgtgaaaa
     4321 tcgtcaacca tgccaggagg ccaggaaaag gcatgtggag cgcggcgaga atactggtaa
     4381 aattttcagg gacgcgcgcc agcttcgccc agccatgctc aatgaatccg tagccgcatc
     4441 aagcggagcg ggagcggcca ccacattttc tgtatggatg acgaatctcg agacaacaaa
     4501 gctgcaaaca tgcgacgacc cttattttgg ctatgccgct tcttcgtcgc cgcgatgccg
     4561 attgtgacgc cgcgattctc acaagtttgg cctcatgccc gctcgagcgc gcatcatttt
     4621 ccggcaatcg atgtgttggc tttgatgggg taggcgagcg ccggacttgt gttcatgccg
     4681 gacacgaaaa tgagattgtt gggataggaa atccagagct tgcgctgttt ccaataatcc
     4741 agtccgaggt tcgccgaagc gcccggcaat tgaacctgga tgaccttcat atattcgttt
     4801 tgataaacgt cctcgccgat acggatttcg tcaaattgat atcgctggtt cggcaatgac
     4861 gtgccgccat tgaacgggct cgttgagtgc aaatgcgttt ttttcatctc gtgtgcgaga
     4921 gcgccccaaa gggctggcgc catacgtgaa acaaacgaaa cattattccc cgtatcgacg
     4981 attgccggcg ttcgctcgcc attgagaaaa atatcgacca tgatcttggt gtcgcgatgc
     5041 tcaaagggca gcgcgaaata ggctcctgtc caaggcacga aagcgtccga acaagcgaat
     5101 tcatcgtaga gcgtcagtct atcgtcgaga aaatcgaact cgacatcgta gtccgacaaa
     5161 atgtcgtcgc ccaatatgcc gacagcgtcg gcttccggca tcggcccgaa gtcgaggctc
     5221 gcaacaattg tgctgcgatt gtgaaaaatc agatctccga atccgagaac gggggcgatg
     5281 acattaaggt aaactaccgc ggcggaggtc tggccgatcc cgagaacggg cttgggcagc
     5341 ggcgcagagt aatcgacctt cagattgaga tcgtccaccg ctttcgcgcc caataatgtc
     5401 gtggccgcgc cagtatcgac gagcatcggc cgggactgcc cggcgacatt ggccatcacc
     5461 gcataatgat tgccgagttc gatcagaggc agttcggcga ctttcaccaa actgcaggcg
     5521 ctggcgtcgt gtcgaagcaa cgtccaggca caaagataaa gagctatgcg aagcgaccgc
     5581 ttcatcgctg actccgcggg gaaaaggccg cggcaactgt atcggacgta gctggcttcg
     5641 ggctggccgc caagcgttct acctcggcgg cgctacccga ttgatttaac tggtggagcc
     5701 agacggaatc gaaccgacga cctcttcaat gccattgaag cgctctccca actgagctat
     5761 gaccccaaac cttcgcgccg ctcaaatctt tgggtggatt tgagtgccag ttccggctga
     5821 cgcggagcgg ctatatagac gcaataaact gccgccacaa gcggccatcg aagcgtcgag
     5881 tgtccgaaaa aaccgcttca cgcttttcgg cccgatgctt acggctcctc gtccggcgcg
     5941 agatcgccgt cgatcaggcc agtgacgtcg tcgctgtcct cttcctcttc ttcgaggaac
     6001 gtatcgtcgt catcgccgcc ctcgtcctca agttcgacat cgtcttccac tgtggcttcg
     6061 aggctcgttt cggccgcttc gacctcatcg agcgagacga tttcgacgcc ctgatcggcc
     6121 gcctactcgt ccttttccac cacaattgcg cgtgcggtgc gggtcgtggc ctggtggact
     6181 gtcccgcact tcgggcagac gaccgggctc ttatacaggt cgaaaaattt ggtgccacag
     6241 ttctggcagg tatgcttgtt gccgagatcg ggtttcgcca c
//
LOCUS       DASWOG010000207         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_41026,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000207 DASWOG010000000
VERSION     DASWOG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41026"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGB93_04235"
     CDS             complement(<1..589)
                     /locus_tag="VGB93_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY0145356.1"
                     /translation="MNIETLAVLGAGAGVAGFVDTLAGGGGLITVPLLLLTGLPPLQV
                     LATNKLQGTFGTLSSTTVLLRKGQISVGEVQAGFVAAFVGGVIGALAVKIANAAALDI
                     LIPLVLATIALYFLFAPRAAEVGGEARLSRAAYLRSIVPAIRFYDGFLGPGTGSSFAL
                     AGVALRAEPLLKATPAAKVLNFGSNLGALVLFLIAG"
     gene            805..>1909
                     /locus_tag="VGB93_04240"
     CDS             805..>1909
                     /locus_tag="VGB93_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0145357.1"
                     /translation="MNLRNIAIIAHVDHGKTTLVDQLLRQSGAFRENQRVAERVMDSN
                     DLEKERGITIMAKATSVAWKDARINIVDTPGHADFGGEVERILSMVDGAIVLVDASEG
                     PMPQTKFVVGKALKIGLRPIVAINKVDRPDARVTEVINEVFDLFAALDATDEQLDFPI
                     LYGSGKMGWMAESPEGPKEDMAPLFDMVLRHVPAPTVEEGSFRMLGTLLEANPYLGRL
                     VTGRVFAGSVKPNQSVKVLDRKGNVVETGRVSKILAFRGIERTPIDEAEAGDIVAIAG
                     LEKFNVADTLGAPDVTEPLQAQPIDPPTLSMTFLVNDSPLAGTEGDKVTSRVIRARLA
                     KEAEGNVALRVEDSPSGESFIVSGRGELQLAILI"
BASE COUNT          396 a          604 c          592 g          317 t
ORIGIN      
        1 ttccggcgat gagaaagaga accaaagcgc cgagattgct gccaaaattg agcacttttg
       61 cagcgggcgt ggccttgagc agcggctcgg ctcgcagggc gacgcccgcg agcgcgaagg
      121 aggagccggt gcccggcccc agaaagccgt cgtagaagcg gatcgccggc acaattgaac
      181 ggagataggc cgcccggcta agccgcgcct cgccgccgac ctctgctgcc cgcggggcaa
      241 agagaaaata gagcgcgatg gtggcgagga cgagaggaat gaggatatcg agcgcggctg
      301 cgttggcgat cttgaccgcc agcgccccga tgacgccgcc gacgaaggcg gcaacgaaac
      361 ccgcctggac ctcgccaaca gaaatctggc ccttccgcaa caagactgtt gtcgatgaca
      421 gggtcccgaa cgtgccctgg agcttgttgg tagctaaaac ctgcaacggc ggcagcccgg
      481 tcagcagcag caacggcacg gtgatcaggc cgccgccccc ggccagcgta tcgacgaaac
      541 cggcgacgcc ggcccccgcg cccagcacag ccagcgtttc aatattcacc aagacgatgc
      601 ccaacccctg agacggtttt gccgccagtt tccaaatcaa tccgattcgt atatgtgatg
      661 ctgcaattgc gaaagggccc accatatgtt gggggccggg ttggcctttc cgcccgcgac
      721 tttccgtaga aatacgtgtg tcatacccat gcgcgatcct gccgcagcgc ggggcgcgcg
      781 ttgaacgctg gatttgagat ttcaatgaac ttgcgcaata tcgccattat cgcccacgtt
      841 gaccacggca aaacgactct cgtcgatcaa ttgcttcgcc aatccggcgc cttccgcgaa
      901 aatcagcgcg tcgccgagcg cgtgatggat tccaacgatc tcgaaaaaga gcgtggcatc
      961 accatcatgg cgaaggcgac gtcggtcgcc tggaaagatg cgcgaatcaa tatcgtcgac
     1021 acccccggcc acgcggattt tggcggcgag gtcgagcgca tcctgtcgat ggtcgatggc
     1081 gccattgtgc tcgtcgatgc ctcggaaggg ccgatgccgc agaccaaatt cgtcgtcggc
     1141 aaggcgctga agatcggcct gcggcccatc gtggcgatca ataaggtcga ccgccccgac
     1201 gcccgcgtca ccgaagtcat caacgaggtt ttcgacctct tcgccgcgct cgacgcgacc
     1261 gacgagcagc ttgatttccc gatcctttac ggctccggca agatgggctg gatggcggaa
     1321 agcccggaag gcccgaaaga agacatggcc ccgctcttcg acatggtgct gcgtcatgtg
     1381 ccggcaccga ccgtcgaaga gggcagcttc cgcatgttgg gcacgctgct ggaggccaat
     1441 ccctatctcg gacggctcgt aaccggccgg gtgttcgccg ggagcgtcaa gccgaatcag
     1501 tcggtcaagg ttctcgaccg caagggcaat gtcgtcgaaa cgggccgcgt gtcgaaaatc
     1561 ctcgccttcc gcggcattga gcgcacgccg atcgacgagg cggaagccgg cgatattgtc
     1621 gccatcgcag gccttgagaa attcaacgtc gccgacacac tcggcgcgcc cgacgtgacc
     1681 gagccactgc aggcgcagcc gatcgacccg ccgaccctat cgatgacctt tctcgtcaac
     1741 gattcgccgc tcgcgggcac ggaaggcgac aaggtcacga gccgcgtcat ccgcgctcgg
     1801 ctggcgaaag aggcggaggg caatgtcgcg ctgcgcgtcg aagattcgcc gagcggtgaa
     1861 tccttcatcg tgtcaggccg cggcgaattg cagctcgcaa ttctgatcg
//
LOCUS       DASWOG010000208         8296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_224776,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000208 DASWOG010000000
VERSION     DASWOG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224776"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGB93_04245"
     CDS             complement(<1..233)
                     /locus_tag="VGB93_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0145358.1"
                     /translation="MLPQDKLDLILRRHTEISDRLASGPDSTTFVALSRELAELDHVV
                     TAIRAYRDEVGEIAGLDSLLDDPKLDAEMRSLA"
     gene            complement(303..2567)
                     /gene="ptsP"
                     /locus_tag="VGB93_04250"
     CDS             complement(303..2567)
                     /gene="ptsP"
                     /locus_tag="VGB93_04250"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0145359.1"
                     /translation="MRTVPGGPRQLLRRLREVVAEPISPQDRLDKIVILIAANMVAEV
                     CSVYVLRADGRLELFATEGLNREAVHLTSMRKGEGLVGLIAESAEALALADAQHHPSF
                     SYRPETGEEIYSSFLGVPILRGGNTLGVLVVQNRARRVYLEEEMEALQTTAMLLAEMV
                     ASGELQSIGLLDTEIALNRPVTLKGVPLADGVGLGYVVLHEPRVIVKQLVAEDVKAET
                     ERLETAITGVRESIDKLIERGDLGHGEHREVLEAFRMFAYDRGWMRRLREAVATGLTA
                     EAAVERVQNDARARLQRQTDPYLRERLHDLDDLANRLLYQLAGRDFVTAQSELPDNAI
                     IVARTMGPAALLDYDRTKLRGIVLEDAGLSSHVAIVARALGIPSVGEVSNILSFVEPG
                     DALIVDGSTGDVQVRPPGDVQNAYAEKARLRAKRQEQYHKLRDVPCVTKDGTAFELHM
                     NAGLLVDLQHVEETGATSIGLFRTELQFMIVSQFPRIEQQYDFYTAAFASAGDRPITF
                     RTLDIGSDKVLPYMTQAEEENPALGWRAIRISLDRPALLRMQLRALLRAGAGRDLRIM
                     FPMVAVVDEFARAKVLVQREIAFLKRHNYGVPREVQLGAMVEVPSLLWQLDEICAQAD
                     FLSVGTNDLIQYMFAADRENKHVSSRFDPLSAPLLRALRGIVEATSRNKTPVTLCGEM
                     GGQPLEALALMAIGFRKLSMSASSIGPVKAALLATDLAAVSKLVGGLIDAKDGARSIR
                     EPLNAFAIAQDIPL"
     gene            complement(2828..4063)
                     /locus_tag="VGB93_04255"
     CDS             complement(2828..4063)
                     /locus_tag="VGB93_04255"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0145360.1"
                     /translation="MGRLVMKFGGTSVAGVDRIRNVARHVQREVAAGHEVAVVVSAMA
                     GKTDELVALCKDASRRYDPREYDAVVASGEQVTAGLLAIVLNDMGIPARSWQGWQVPI
                     LTTEAASAARISAVNGEHILAGFLAKEVAVVAGFQGMSAATGRIATLGRGGSDTSAVA
                     IAAAIKAERCDIYTDVDGIYTTDPRVVPKARRLDRIAFEEILEMASLGAKVMQVRSVE
                     LAMMKRVKTYVRSSFDDPDSPSPGTLICDEEDIVEQQVVTGIAFSRNEAQITLRRVAD
                     RPGVAAAIFMPLADAHINVDMIVQVASDDTVTTDMTFTVAANDYDRARAILEKARATI
                     GYAALQGAVDVAKVSAIGIGMRSHAGVAALAFKSLSEKGINIRAITTSEIKFSILIDA
                     AYTELAVRTLHTLYGLDQV"
     gene            4207..4977
                     /gene="ubiG"
                     /locus_tag="VGB93_04260"
     CDS             4207..4977
                     /gene="ubiG"
                     /locus_tag="VGB93_04260"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY0145361.1"
                     /translation="MTGGETPQEGASVNLEEVARFDRLAATWWDLKGPMKPLHRFNPV
                     RVDYLKQFLVNRPRRDSRPTANPGRPLDGLEILDIGCGAGILSEALARLGAEVTGVDP
                     APANIKVAQHHAEQSGLRIDYLETTAEALAEKKLRYDVVLAMEVVEHVRDVKAFVATA
                     ASLVRPGGVMVAATLNRTLKSYALAIVGAEYILRWLPRGTHDWQHFVTPRELRLALRS
                     GGLKILDETGVVYHPLTDTWTRSSDMDVNYIIAAERKV"
     gene            complement(4947..6929)
                     /locus_tag="VGB93_04265"
     CDS             complement(4947..6929)
                     /locus_tag="VGB93_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEY0145362.1"
                     /translation="MPQTFNAARAPDAPAFAPVPSFGRDATLNDAANLLRQGRKAEVL
                     SALATLDPTMISSPDRCHLAGLIPVGAEAWAGAVPWFAQARKLRPDFVDAIVRLARVL
                     QRLGRFEEAAAAFDEAEKLGVSDPSVYYNKGLVLHALRRDGQAITAFDHALRLQPAYP
                     EALRAGAIILSQSGNSDGALKFLEEALRLKPDYFEALLDRANILNALKHIDAACEAYR
                     TALGRFPGNPDLLNNLGVALIETGDLKGAFEALDQAVAIKPELAEAWLNRATVLMKTV
                     GPDAALQSFDRALALRPDYQAALVVRGVALKELEEFDAAEAAFDAALALAPDSAHARN
                     NKGVLQLLRGNFHDGWENYEYRWGCGLTPHNQLKFPIPQWQGNSHPGEKLIVYGGEGL
                     GDALQFCRYLPLLAKAGIDVTFFCRSKLLRLMRGLTPQVRCVDDLGPYERFDSQIALS
                     SLPFAFGTDLTTIPSRASYLATEPALVSKWAEWLISRGNSAAFKVGLCWQGNLDPKAD
                     PSRSIPLAHFAPLAGVKDFRLFSLQKGEGAEGLAAAPQLGIETLGADFDAGPDAFIDT
                     AAVIQTLDLIVTCDTSVAHLAGALGRPTFLLLRKVPDWRWMLDREDTPWYPTMRLFRQ
                     RTRGDWDETMLRVADAAKALHDYNSGLQTLRSAAMM"
     gene            complement(7008..7427)
                     /locus_tag="VGB93_04270"
     CDS             complement(7008..7427)
                     /locus_tag="VGB93_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY0145363.1"
                     /translation="MIKYALKCANGHEFDSWFPSAASFDEQVARGQVVCPVCGVAEVG
                     KAIMAPAVSHGDKIESPLAQGDEAKRRAELRALRHYVMGVTEDVGTRFPDEARKIAEG
                     SSEERPIRGEATREEAKALLDEGIHILPLPFASEDMN"
     gene            complement(7424..7696)
                     /gene="grxC"
                     /locus_tag="VGB93_04275"
     CDS             complement(7424..7696)
                     /gene="grxC"
                     /locus_tag="VGB93_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY0145364.1"
                     /translation="MPQPHIVIYTTASCPYCHRAKALLNERGLAFEEISVDGDPAARA
                     TMTARANGLYTVPQIFFGDRHIGGCDDLHDLDARGELQTVLADAAQ"
     gene            complement(7733..8296)
                     /locus_tag="VGB93_04280"
                     /pseudo
     CDS             complement(7733..8296)
                     /locus_tag="VGB93_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490421.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         1651 a         2606 c         2566 g         1473 t
ORIGIN      
        1 tccgcgaggc tgcgcatctc ggcgtcgagc ttcggatcgt cgaggagcga atcgagtccc
       61 gcgatctcgc cgacttcgtc gcgataagcg cggatcgctg tgacgacgtg gtcgagttcc
      121 gccagttcgc gcgaaagcgc gacgaaggtt gtcgaatcgg gacccgaggc gagccggtcc
      181 gaaatttcgg tgtggcggcg cagaatgaga tcgagcttgt cttgcggcag catgactttc
      241 taagagctta tcgcggccac cccgtcaatg ccgcggctag agcgcaggcc tggagcggcg
      301 gtctagaggg gaatgtcctg cgcaatcgcg aatgcgttga gcggctcacg gatggaacgc
      361 gcgccgtctt tggcatcgat cagaccgccg accaatttgc tcaccgccgc aaggtcggtc
      421 gccagcaacg ccgccttgac cgggccgatc gaggacgcgg acatcgacaa tttgcggaag
      481 ccgatcgcca tcagcgcgag cgcctccagt ggctggccgc ccatttcgcc gcagagagtc
      541 accggcgtct tgttgcgcga agtagcttcg acgattccgc gcaaggcccg cagcagcggc
      601 gccgacaggg gatcgaaccg gcttgagacg tgtttattct cgcgatcggc ggcgaacata
      661 tattgaatca agtcattcgt gccgaccgac agaaaatcgg cctgggcgca aatttcgtcg
      721 agctgccaga ggagcgacgg cacctcgacc attgcgccga gctgcacttc gcgcggcacg
      781 ccgtaattat ggcgcttcag aaaagcgatt tcccgctgaa ccaaaacttt cgcccgggcg
      841 aattcgtcaa cgacggcgac cattggaaac atgatccgca aatcgcggcc ggcgccggcc
      901 cgcagcagcg cgcgcaattg catccgcagc aaggccggac gatcgagcga aatacggatc
      961 gcgcgccagc cgagcgcagg attttcttcc tcggcctgcg tcatataggg cagcacttta
     1021 tccgagccga tatcgagcgt gcggaaggtg atcggccgat ccccggcaga tgcgaaagcc
     1081 gctgtgtaga aatcgtattg ctgttcgatc cgcgggaatt gcgacacgat catgaattgc
     1141 aattcggtgc ggaacaggcc gatcgaggtc gcgcccgttt cttccacatg ctgaagatcg
     1201 acaagaagcc cggcgttcat atgcaattcg aacgcggtgc cgtccttggt tacgcaagga
     1261 acgtcgcgca gcttgtgata ttgctcctgc cgcttggcgc gcagccgggc cttttcggca
     1321 taagcgttct ggacatcgcc gggcggccgc acctgcacat cgccggtcga cccatcgacg
     1381 atcaaggcgt cgccgggctc gacgaaggag agaatgttgg agacttcgcc aaccgacggg
     1441 atgccgagcg cccgggcaac gatcgcgaca tggctcgaaa gacccgcgtc ctccagcacg
     1501 ataccgcgta atttggtgcg atcgtaatca agcagcgccg ccggtcccat cgtgcgtgcg
     1561 acgatgatgg cgttgtcggg cagttccgat tgcgcagtta cgaaatcgcg gccagcgagc
     1621 tgataaagta gccggttggc gagatcgtcg agatcgtgca ggcgttcgcg cagataaggg
     1681 tcggtctggc gttgcaaccg tgcgcgcgca tcgttttgca cgcgttcgac tgcggcctca
     1741 gccgtgagac ccgtcgcgac cgcttcgcgc agccgacgca tccaaccgcg gtcgtaagcg
     1801 aacatgcgga aggcttcgag cacttcgcga tgttcgccgt ggccaaggtc gccacgctcg
     1861 atcaatttgt cgatcgattc gcgcacgccg gtgatcgcgg tttcaagccg ctcggtctcg
     1921 gctttgacgt cttcggcgac gagctgcttt acgatgacgc gcggctcatg cagcacgacg
     1981 taaccaagcc cgacgccatc ggcaagcggc acgcccttca gcgttaccgg ccggttgagc
     2041 gcaatctccg tatcgagcag gccgatcgac tgcagttcgc ccgaagcgac catttcggct
     2101 agcagcatgg cggtcgtctg cagcgcctcc atctcctctt cgagatagac gcggcgtgca
     2161 cgattctgca cgacgaggac gccaagcgta ttaccgccgc gcaagatcgg gacgccgagg
     2221 aacgaggaat aaatttcttc gcccgtctcc ggtcggtagg aaaacgaggg atgatgctgc
     2281 gcatcggcca gcgccaatgc ctcagccgat tcggcgatca ggccgacaag gccttcgccc
     2341 ttgcgcatcg aagtgagatg gaccgcctcg cggttcaggc cttccgttgc gaaaagctcg
     2401 agccgaccgt cggcgcgcag gacgtaaacc gagcaaacct cggccaccat attcgcagcg
     2461 atgaggatga cgatcttatc gagccggtct tgcgggctga tcggctcggc cacgacctcg
     2521 cgcagccgcc gcagcaattg acgcggccct cccggcacag tgcgcaccgc cttgtcttgt
     2581 tccggccctt cggcctggga ttcataggtc ggcacaagct tatccgcagg gggactgaaa
     2641 gcgatcgatt aggagaaacg cccgccgcac gctcctccaa cgatggcgct gcggcgatca
     2701 gacgggtccg caagaactcc gccatagtta agagccggcg ccagcgaaac gcgacacctt
     2761 cctatagcga atgcaaagat cagaccgcaa gcgctcaaac gagaggccgc gaaaaagtta
     2821 tgcggattta gacctgatca agaccataaa gcgtgtgcaa cgtacggacc gcgagttccg
     2881 tataggcggc gtcaatcaat attgaaaatt tgatttcaga tgtcgtaatc gcgcgaatgt
     2941 tgatgccctt ttcagacagc gatttaaacg ccagcgccgc gacgccggcg tggctgcgca
     3001 tgccgatgcc gatcgccgaa accttggcaa catcgacggc accctgcaat gcagcatagc
     3061 cgatggtggc ccgtgctttt tccaaaatcg cgcgggcgcg gtcgtaatcg ttggcggcaa
     3121 ccgtgaacgt catatcggtc gtcacagtgt cgtcggacgc gacctggacg atcatatcca
     3181 cgttgatatg cgcgtccgcg agcggcatga aaatcgccgc ggccacgccc ggccggtcgg
     3241 cgacgcggcg caaggtgatt tgcgcctcgt tgcgtgaaaa ggcgataccg gtcacgacct
     3301 gctgctccac gatatcctcc tcatcgcaaa tcaaagtgcc ggggctcgga ctgtcgggat
     3361 catcgaacga ggagcgcaca taggtcttta cgcgcttcat catcgcgagt tcgaccgagc
     3421 gcacttgcat caccttggcg ccgagcgagg ccatttccaa gatttcttca aaggcgatac
     3481 ggtcaagccg gcgcgctttc ggcacgacac gcgggtcggt cgtgtatatg ccgtccacat
     3541 ccgtgtagat atcgcagcgt tcggccttga tcgccgcggc gatcgcaacc gcactcgtgt
     3601 cggagccgcc ccggccgagc gtcgcgatac ggcccgtggc ggcgctcatg ccctggaagc
     3661 cggcgacgac ggcaacttcc ttggctagaa aacccgcgag aatgtgttcg ccgttaaccg
     3721 ccgagatacg cgcggcgctg gctgcctccg tcgtgagaat cggcacctgc cagccttgcc
     3781 aggaccgcgc cgggatgccc atatcgttga gaacgatggc gagcagaccg gcggtcacct
     3841 gctcgcccga agcgacaacg gcatcatatt cgcgcgggtc gtagcgacgg gaggcgtctt
     3901 tgcagagcgc gacaagctca tcggtcttgc cggccattgc cgaaacgacg accgcgactt
     3961 cgtggccggc cgcaacttcg cgttggacat ggcgcgccac attgcggata cggtcgacgc
     4021 ctgcgacgga ggtcccaccg aatttcatca caaggcggcc catcggatcc ggcagcgctt
     4081 tcttgattcg ggctctaaaa tggttcaccg cggcggccgg gtcggccgcg gcaatgcggc
     4141 gcgtttacac cacgaccccg gcggcgaagt caatgaacgg cgcatgcggc cagcaggagg
     4201 gttaaaatga ccggggggga gacaccgcaa gagggagcca gcgtcaatct ggaggaagtc
     4261 gcgcgttttg accgtttggc ggcgacctgg tgggatttga agggcccgat gaagcccctc
     4321 catcggttca atcctgtgcg ggtcgattac ctcaaacagt ttctcgtcaa tcggccgcgc
     4381 cgtgacagcc ggccgacggc caatcccggc cggccgctcg acgggcttga aatcctcgat
     4441 atcggctgcg gcgccggcat tctcagcgaa gccctggcgc gccttggcgc cgaagtcacg
     4501 ggcgtcgatc cagccccggc caatatcaag gttgcgcaac accatgccga gcaatcgggc
     4561 ttaagaatcg attatctcga aacgacggcc gaggcgttgg cggaaaagaa gctgcgctac
     4621 gacgtcgtcc tcgcgatgga ggtcgtcgag cacgtccgcg acgtcaaagc ttttgttgcg
     4681 acagcggcgt cgctggtgcg gccgggcggt gtcatggttg ccgcaacgct caatcggacg
     4741 ctgaagagct acgcgctcgc gatcgtcggc gccgaatata ttttgcgctg gctgccgcgc
     4801 ggcacacacg actggcagca tttcgtgacg ccacgcgaat tgcgtctcgc gttacgcagc
     4861 ggcggcctga agattctcga cgaaaccggc gtcgtctatc acccgctcac cgacacctgg
     4921 acacgcagct cggatatgga cgtgaattac atcatcgcgg ctgagcgcaa ggtctgaagc
     4981 cccgagttgt aatcatgcaa ggctttggcg gcgtccgcga cgcgcagcat cgtctcgtcc
     5041 caatcgccgc gcgtgcgctg acggaagagc cgcatggtcg gataccaggg cgtatcttcg
     5101 cggtcgagca tccagcgcca atcgggcacc ttcctgagta gcagaaaggt cggacgcccc
     5161 aaggcgccgg cgagatgcgc cacagacgtg tcgcaggtaa caatcagatc aagcgtctgg
     5221 atcaccgcag ccgtatcgat aaatgcatcc ggcccggcat cgaaatcggc gccaagcgtc
     5281 tcgatgccca gctgcggcgc ggcagccaag ccctccgcgc cctcgccttt ctgcaggctg
     5341 aagaggcgga aatccttaac gccggccagc ggcgcgaaat gcgcgagcgg aatcgaccgt
     5401 gagggatccg ccttcggatc gagattgccc tgccagcaca gaccgacctt gaaggcggcg
     5461 gaattgccgc ggcttatcag ccactcggcc catttcgaca cgagtgcggg ttcggtggcg
     5521 aggtaagacg cacggctcgg gattgtcgta agatcggtgc cgaacgcgaa cggcagactt
     5581 gaaagcgcga tctggctgtc gaatcgttcg tacggcccga gatcgtcgac acagcgcacc
     5641 tgcggagtca agccgcgcat cagccgcagc agcttgctgc ggcagaagaa ggtgacatca
     5701 atgccagcct tggcgagcag cggcagatag cgacagaatt gcagcgcgtc gccgagaccc
     5761 tcgccgccat aaacgatcag cttctcgccc ggatggctgt tgccctgcca ctgcggaatc
     5821 gggaatttga gctggttatg gggcgtgagg ccgcagcccc agcgatattc gtagttttcc
     5881 cagccatcgt ggaaattgcc gcgcaataat tgcagcacgc ccttgttgtt gcgggcatga
     5941 gcactgtcgg gagcaagagc gagggcagca tcgaaggcgg cttccgccgc gtcgaattct
     6001 tccagttctt tcagcgcgac gccacgcacg accagcgccg cctgataatc cgggcgaagc
     6061 gccagcgcgc ggtcgaagga ttgaagggcc gcatccgggc cgaccgtttt catcaacacc
     6121 gtggcgcggt tgagccacgc ttcagccaac tcaggcttta tcgcaacggc ctgatcgagt
     6181 gcctcgaacg cgcctttgag atcgccggtc tcgatcaggg ccacgccaag attattgaga
     6241 aggtcgggat tacccgggaa ccgcccgagc gccgtcctat aagcctcgca ggccgcgtca
     6301 atgtgcttca atgcgttgag gatattggcg cgatccagca gcgcctcgaa ataatccggc
     6361 ttgaggcgca gggcctcctc gaggaatttc aacgcgccgt cggaattgcc gctctgcgaa
     6421 aggataatcg cgccggcacg caaagcttcg ggataagccg gctgcagccg gagcgcatga
     6481 tcaaaagccg taatcgcttg accatcgcgg cggagcgcgt gcaggacgag acctttgttg
     6541 taatagacgc tcggatcgct aacgccgagt ttttccgcct cgtcgaaggc tgcggcggct
     6601 tcttcaaagc gccccaatct ttgcaacacc cgcgcgagtc gcacgatcgc atcgacaaag
     6661 tccgggcgca gcttgcgcgc ctgcgcaaac cacggcacgg cgccagccca ggcctcggcc
     6721 ccgacaggaa tgagaccggc gagatgacaa cgatccgggc tgctgatcat ggtcggatcg
     6781 agtgtcgcga gggctgaaag aacttcggcc ttacgcccct gtcgcagcaa attcgcggca
     6841 tcgttcagcg tcgcatcgcg gccaaaactc ggcaccggag caaacgccgg tgcatccggc
     6901 gcgcgtgctg cattgaaggt ctgaggcatg ggaagctccg cccagtaaag gcggcagtgt
     6961 cggcacaccg gcttgcgcca gacttacgta ggcgtcgcga taaagaatta attcatatct
     7021 tccgacgcga acggcagcgg cagtatatgg ataccttcgt cgagaagcgc cttggcctct
     7081 tcgcgcgtcg cctcaccacg aatcggccgc tcctcgctcg atccctcggc gatcttgcgc
     7141 gcctcgtcag gaaagcgcgt cccgacatct tcggtgacgc ccatcacata gtgccgcaga
     7201 gcgcgcagct cggcgcggcg cttggcttcg tctccctgcg cgagcggcga ttcgatcttg
     7261 tcaccgtggc tgacagccgg cgccatgatt gccttgccga cctcggccac gccgcagacc
     7321 ggacatacca cttggcctcg cgcaacctgt tcgtcaaagc tcgccgcgct tggaaaccac
     7381 gaatcgaact catggccgtt ggcacacttc agggcatatt tgatcattgc gccgcgtcgg
     7441 ccaaaactgt ctggagttcg ccccgtgcat cgagatcgtg cagatcgtca cacccaccga
     7501 tatggcggtc gccaaagaaa atctgcggca cggtgtaaag gccgttggcc cgcgccgtca
     7561 tcgttgcgcg cgctgccggg tcgccatcga cggaaatctc ctcgaacgcc agcccgcgct
     7621 cattgagcaa cgccttggcg cgatggcaat aggggcagga agcggtggtg tagataacga
     7681 tatgcggctg cggcatcgat tggacctttt gatttctctt acatatggga ttttagcccg
     7741 cgctcacaac cctggcgaaa accaggacat cgacgcgcgt ggcaccgccg cgtagcaagg
     7801 cgcgcgccgc cgcattcagc gtcgcgccgg aagtgagcac atcgtcgatc agcaacacgt
     7861 tgcggcccat gatggcgagc gaccggcctc ggacaccttg aaggcgccct gcacattatc
     7921 ggcacgctgg ctgcgcgtca ggccaacctc cggcacggtg cgtttgactc gcgcaaacgc
     7981 gaacggctca accctcttgc cgctcgccct gccgatcacc tcggccaaag cgttcgcctg
     8041 attgccgcca gatgaggcgc cgccgatgca acggcactgg aatcagcaga tccgcttcgg
     8101 caagcagctc cgcgccggca cgcgccatcc agaggccgag cggtttggcg agttccatcc
     8161 ggtcgccgta tttcaaccga tggatcagcg cgcgaaccgg gccgtcctcg aaacgcgcga
     8221 cggcgcgggc acgctgatag acgggcgagt tggccattgc ctccggagat tgcaggcccg
     8281 gaacgccgag atcata
//
LOCUS       DASWOG010000209         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_334501,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000209 DASWOG010000000
VERSION     DASWOG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334501"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGB93_04285"
     CDS             <1..733
                     /locus_tag="VGB93_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0145365.1"
                     /translation="HSIQGLGPDGCEFVLVFNDGNFSEDSTFLLSDWMRHTPSEVLTK
                     NFNAPASAFANLPKKDLWIFNAPLPGSLGADLAQSSQPFVPDSYSFPLMDQPPIRTKS
                     GTVRIADKNNFKASDVMSAALVEIEPGGLRDLHWHPTSDEWQYYIQGTARMTVFAGEG
                     RANTVDFAASDVGYVPRAMGHYIENTGKDTLRFLEVFAVGQYADISLTSWIANSPNEL
                     VASHLNIDENIVRGLPRTKTPVVPA"
     gene            941..>1981
                     /locus_tag="VGB93_04290"
     CDS             941..>1981
                     /locus_tag="VGB93_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145366.1"
                     /translation="MSTLVSSAPSAGGGLLDRSRTIAGPRFNRWLVPPAALAIHLCIG
                     MAYGFSVFWLPMTKLLPAANAAACKGQGFFAQLGTTSCNWTVPNVTNVFEIFIAMLGI
                     SAAIWGGWLEHAGPRKAGFYAAICWGGGLVLLGLAVTTHQLWLIYLVAVFCGIGQGLG
                     YITPVSTLIKWFPDRRGMATGFAIMGYGGGAMIGAPLAVWLMGKFGANGIPGVSQALI
                     AMGIIYFIAMSIGALGFRVTPSGWLPAGWTPPAAGTTKRFISHNSVHLSQAWKTPQFW
                     FIWGVLFCNVTAGIAVISVASPMFQDIFGAKLVGVESAVTLTAAQKASIAAAAAGLVG
                     LNSLFNSLGRLFW"
BASE COUNT          375 a          587 c          573 g          446 t
ORIGIN      
        1 gcattcaatc caggggctcg ggccggacgg ctgcgaattc gtgctcgtct tcaacgatgg
       61 aaatttctcc gaagacagca cattcctgct cagcgactgg atgcgccaca cgccgtcgga
      121 agttctcact aaaaatttca atgcgccggc gagcgccttt gccaaccttc cgaaaaagga
      181 tttgtggatt ttcaacgcgc cgctgcccgg ctcgctgggc gccgatctgg cccaaagcag
      241 ccagccgttt gtccccgatt cctattcgtt cccgctcatg gatcagccgc cgatacgaac
      301 caaaagcggc accgtacgca ttgccgacaa gaacaatttt aaagcctcgg acgtcatgtc
      361 cgcagcactc gtcgaaatcg agcccggcgg tctgcgcgat ttgcattggc atccgacatc
      421 ggacgaatgg caatattaca tccaaggcac ggcgcgcatg actgtcttcg ccggggaagg
      481 ccgcgccaac actgtagatt tcgccgcaag cgacgtcggt tatgtgccgc gcgccatggg
      541 ccattacatc gagaataccg gcaaggatac gttgcgcttt ctggaggttt tcgccgtcgg
      601 ccaatatgcc gatatttcgc tgacctcgtg gatcgcgaat tcgccgaacg aactcgtcgc
      661 ttcacatttg aacattgacg agaacattgt gcgcggcttg ccacgtacaa aaacgccagt
      721 cgtccccgcg tagaactcaa cgctgccgtg agagcgaaag ctgaaacggc agcgtttttt
      781 gcgctgcaaa ctgattgata gctgaagatg tgaggacaat tattccttgc ccggcacaag
      841 caattgtgca gtttaattat ccgtcaaaat gcaccgaaaa tggtgccgaa gaagacgcgg
      901 tcggtacacc cggccgtttc ttatggaatg aggaacgccc atgagcactc tggtatcttc
      961 cgcaccctcc gcgggtggcg ggcttctcga tcgttcgcgc accattgccg ggccgcggtt
     1021 caaccgctgg ctcgtacccc cggcggcact cgcgattcat ctctgtatcg gtatggctta
     1081 cggtttctcg gtattctggc tgccgatgac gaagctgctg ccggccgcca acgccgccgc
     1141 ttgcaagggc cagggcttct ttgcccagct tgggacgaca agctgcaatt ggactgttcc
     1201 taatgtaacg aatgtctttg aaatcttcat cgcgatgctc ggcatttcgg ccgcgatctg
     1261 gggtggctgg cttgaacatg ccggcccgcg gaaggcgggc ttttacgctg ccatctgctg
     1321 gggcggcggc cttgtgctgt tgggcctcgc ggtaacgaca catcagcttt ggctcattta
     1381 tcttgtcgca gttttctgcg gaatcggtca gggcctcggt tacatcacgc cggtttcgac
     1441 gctgatcaag tggttcccgg accggcgtgg catggcaacc ggcttcgcca tcatgggata
     1501 tggcggcggt gcgatgatcg gcgcaccgct tgcggtctgg ctcatgggca agttcggcgc
     1561 caatgggatt cccggcgttt cacaggcgct gatcgcgatg ggcatcatct atttcatcgc
     1621 gatgagcatc ggcgctctcg gcttccgcgt cacgccgtcc ggttggctcc cggcgggatg
     1681 gacaccgcct gcggcgggca cgaccaaaag attcatcagt cataacagcg tgcatctcag
     1741 ccaggcttgg aagacaccgc aattctggtt catctggggt gtgcttttct gcaacgtgac
     1801 ggccggcatc gcggtgatct cggtcgcgag cccgatgttc caggacattt ttggcgccaa
     1861 actcgttggt gtagagtctg ccgtgacgtt gacggccgcg caaaaggcgt cgatcgcggc
     1921 cgccgccgca ggtctcgtcg gcctcaacag cctgttcaac tcgcttggcc gcctcttttg
     1981 g
//
LOCUS       DASWOG010000210         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_298370,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000210 DASWOG010000000
VERSION     DASWOG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298370"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGB93_04295"
     CDS             complement(<1..657)
                     /locus_tag="VGB93_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0145367.1"
                     /translation="MSTPKEIGRRGFLLRAAGGLAAFSLGGCDDSPSQNPKVIDILES
                     AENLTRVSQKAITGAMTLAPEYTEADISTDFRANGTTDPGDDKYKALAKNNFAAYRLK
                     VGGLVETPAQFSLADLRAMPSRTQITRHDCVEGWSCIGKWKGVQLSLVLDKVRPKSNA
                     RYVMFYSADPMDDDDSGPNSLYYESIDMQGAYHPQTILAYDMNDQTLPVPNGAPMRLR
                     D"
     gene            complement(644..1447)
                     /locus_tag="VGB93_04300"
     CDS             complement(644..1447)
                     /locus_tag="VGB93_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY0145368.1"
                     /translation="MAPLRLFYRHPLWIRVTHWLNAICMAVLLMSGLQIFNAHPALYW
                     GQKSDFDHPIFSISSVEDAQGNESGVTTVAGHSFNTTGVLGLSRGGDRAFPDWATLPH
                     EQWLAIGRRWHFFFAWIFVINGLIYVATSLIGRHLRGDLVPTRADLVHIGRSIWEHVR
                     LRFPKGDEAKRYNVLQKLAYLIVVFVLGPVMVLAGLTMSPRLDASYPILLEIFGGRQS
                     ARTIHFICAFSFLGFFVIHIVMVLLSGVFNNLRSMITGWYNLGENREHA"
     gene            complement(1580..1983)
                     /locus_tag="VGB93_04305"
                     /pseudo
     CDS             complement(1580..1983)
                     /locus_tag="VGB93_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334396.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            2414..>2718
                     /locus_tag="VGB93_04310"
     CDS             2414..>2718
                     /locus_tag="VGB93_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145369.1"
                     /translation="MVGELLRLRQTLPHGSGAAGRLSLLRREIVGIAITGITAAPHPA
                     SGDPRVRVYLGETKLDHMLAGGLAIGALHEITAAGPGDAPAAAGFALALAARFAASR"
BASE COUNT          599 a          794 c          793 g          532 t
ORIGIN      
        1 gtcgcgcagc cgcatcggcg cgccgttagg aacgggcagc gtctgatcgt tcatgtcata
       61 ggcgaggatc gtctgcgggt gataggcgcc ctgcatatcg atgctctcgt aatagaggct
      121 gttcggtcct gaatcgtcat catccatcgg atcggcagaa tagaacatga cataacgtgc
      181 gttcgatttc gggcgcactt tatcgagcac gagcgagagc tggacgccct tccatttgcc
      241 gatgcaactc cagccctcga cgcaatcgtg ccgggtgatc tgcgtgcgcg acggcatcgc
      301 ccgcagatca gcaagcgaga attgcgcagg cgtctcgaca agaccgccga ctttgagccg
      361 gtaagcggcg aaattgtttt tcgccagcgc cttatattta tcgtcgcccg gatcggtcgt
      421 gccattggca cgaaaatccg tagagatgtc cgcttcggta tattccggcg cgagggtcat
      481 cgcgccagta atcgccttct gcgacacgcg tgttagattt tcggcgcttt cgagaatgtc
      541 gataaccttc ggattttgcg acgggctgtc gtcgcagccc ccgagcgaga aagcggcaag
      601 gccgcctgcc gcacgcagca gaaagccgcg ccgcccgatc tccttaggcg tgctcacgat
      661 tttctccgag attgtaccag ccggtgatca tcgaacgcag attgttgaac acgcccgata
      721 agagcaccat gacgatatgg atgacgaaaa agccgaggaa tgaaaatgcg caaatgaaat
      781 ggatcgtccg cgccgattgc cggccgccga aaatctcaag cagaatcgga taactcgcat
      841 cgagccgcgg cgacatagtg aggccggcga gcaccatgac cgggccgagc acgaaaacga
      901 caatgagata ggcaagtttc tgcagcacat tgtagcgttt cgcctcatcc cccttcggga
      961 aacgcagccg cacatgttcc cagatagagc ggccgatgtg tacgaggtcg gcgcgcgtcg
     1021 gcacgagatc gccgcgcaga tgccgcccga tcaacgaggt cgcgacatag atcaggccat
     1081 tgatcacgaa aatccaggcg aagaagaaat gccagcgccg cccgatcgcc agccattgct
     1141 catgcggcaa cgtcgcccaa tcggggaaag cgcgatcgcc acccctcgaa aggccaagta
     1201 cgcccgtcgt gttgaagctg tgtccggcga ccgtcgtgac gccgctctcg ttgccttgcg
     1261 cgtcttcgac ggaactgatc gaaaagatcg gatggtcgaa atccgatttc tgcccccaat
     1321 agagcgctgg atgagcattg aaaatctgca gcccgctcat caggagcaca gccatgcaga
     1381 tggcgttgag ccaatgcgtc acccgtatcc agagcggatg gcgataaaac aggcggagcg
     1441 gggccattgg cgaggtctcc ggacgactcc ccctgctacg cagccgaagg ccgacgcgtt
     1501 acggtggtcg cgacttcgtg aacgacgccc tttacgagcc gtggaccagc gcagttttca
     1561 ggcggaacgg cctcgacagc tattcattca acagcttatc gaaataggca atcgtttctt
     1621 taagcccttg ctgaagcttt acacgcggct cccattgtag ctcagcccgc gcgatggaaa
     1681 tgtcaggctg ccgctgtttg ggatcgtccg acggcaacgg ctcctgaatc agtggagact
     1741 tgctgccggt gagttcgatg acgagttcgg ccagttgccg gatcgtaaat tcgtcaggat
     1801 tgcccatgtt caccggccca ataagttcgg gccgcgattc catcaaacgg atcatgcatt
     1861 ctatgagatc gtcgacgtaa caaaagatct tgtctgctcg ccgtttccat aaagcgtaat
     1921 cggatcgccc cggagtgcct gcacaatgaa gttggagacc acccgcccgt cgttcgggtg
     1981 catgttcgcc agttgagcgc tgctcccgcg agcgtgatac taaaaatccc gattaggccg
     2041 agttcgccaa acgtagtttg gcggttcaca tcaatggcaa gaacccgctc aagaattcgg
     2101 caaaaaaatg cgccgagacc gaccagcgct atcacgagaa tgagggtgat gaatgcgatg
     2161 agaagcatat tggccgttct tcagaaggcg agattgtccg ggaagcgcta gcggagctgt
     2221 acccgaggtg ggagtcgtct acaatccgct agatcgatcg acatgggcga gcagagagcg
     2281 agctttgggc catcgaaaga tcccgtgctt agcggtaaaa actgggacac tacccgactc
     2341 cctcctccct tgactccctc ctcaaaaaga ggaatccata agagaacaaa tatggaacat
     2401 tgggtcgaga gatatggtcg gggagctttt gcggcttcgc caaacccttc cgcatgggag
     2461 cggcgcagcg gggcggctca gccttttgcg tcgggagatc gttggtattg cgatcaccgg
     2521 catcaccgcg gcgcctcatc cggcgtctgg cgacccgcgc gtgcgcgtct atctcggcga
     2581 gacaaagctc gatcacatgc ttgctggcgg ccttgcgatt ggcgccctgc atgagatcac
     2641 cgctgccggt cccggcgatg ccccagccgc cgccggcttc gccctggcac tggccgcgcg
     2701 ctttgccgcc agccgcaa
//
LOCUS       DASWOG010000211         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_242991,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000211 DASWOG010000000
VERSION     DASWOG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242991"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /gene="ppdK"
                     /locus_tag="VGB93_04315"
     CDS             complement(<1..1892)
                     /gene="ppdK"
                     /locus_tag="VGB93_04315"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0145370.1"
                     /translation="MTKWVYTFGGSTAEGSSQMHALLGGKGANLAEMANLGLPVPPGF
                     TITTDVCAYFYANAKTYPSNLKPQVDAALKQIEAITGRELGDAQRPLLVSVRSGSRAS
                     MPGMMDTVLNIGLNDATVEALARDAGDARFAYDSYRRFIQMYSSVVLGVAHHHFEEAI
                     EIFKEDNSLNLDTDLSAADWQKIISIFKTKIAEVHGSPFPQEPQSQLWGAIAAVFGSW
                     HNPRAKIFRQLHNIPESWGTAVTVQAMVFGNMGDTSATGVAFTRNPSIGAKELYGEFL
                     INAQGEDVVAGVRTPQNITEVARIAAGSEQPSMETALPEVFDQFVAVADQLERHYRDI
                     QDVEFTVERGKLWMLQTRSAKRTAKAALRAAVEMAQEGLISQQEAVLRIEPGSLDQLL
                     HPNVAPESERDVIATGLPASPGAASGEIVFNAEEAEQLRSAGRKAILVRVETSPEDIH
                     GMHAAQGILTTRGGMTSHAAVVARGMGKPCVSGVGALRIDSERGILSAGPVTLRRGDI
                     VTIDGTSGKVMRGAIEMRQPELSGEFGTIMQWADKLRRLRVRANAETPADAEAARRFG
                     AEGIGLCRTEHMFFGAARIAAVREMILADDEKSRRAALAKLLTFQRGDFERLVEIMSG
                     LPVTISL"
     gene            complement(1970..>2520)
                     /locus_tag="VGB93_04320"
     CDS             complement(1970..>2520)
                     /locus_tag="VGB93_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY0145371.1"
                     /translation="CVERLRYRWPSFIVLGALVATMGVAALVQAVAATIVSVYIIAFF
                     MIVAGGGEIVFANCSRSWSRFFLWVIAGLAYIVVGAFALAQPLMAAALFTLLLGVAMF
                     VTGLIRIYVGTHLNNDVRTIVVVAGVVTSLVGLVVLIGWPANSLAILGVLLGLDLLFW
                     GGSWITLGLRMKAHLNTLPRHA"
BASE COUNT          513 a          793 c          776 g          438 t
ORIGIN      
        1 aggctgatcg tcaccggcaa gccggacatg atttcgacca atcgctcgaa gtcgccacgc
       61 tgaaacgtca gcaatttggc gagcgcggcg cggcggctct tttcgtcgtc ggcgaggatc
      121 atttcgcgca cggcagcaat acgcgcggcg ccgaagaaca tatgttcggt ccggcaaagg
      181 ccgatgcctt cagcaccgaa gcggcgcgct gcctcggcgt cggctggcgt ctcggcattg
      241 gcgcgcacgc gcaggcggcg cagcttgtcg gcccattgca tgatcgtacc gaactctccc
      301 gacaattcgg gctgccgcat ttcgatcgcg ccgcgcatca ccttcccgct cgtgccgtcg
      361 atggtcacga tgtcgccgcg ccgcagcgtc accggaccgg ccgacaggat gccacgctcg
      421 gaatcgatcc gcagcgcgcc gacaccggag acgcagggct tccccatgcc acgcgcaacc
      481 accgccgcgt gagaggtcat gccgccgcgc gtcgtcagaa tgccttgcgc agcgtgcatc
      541 ccatgaatgt cttcgggact cgtctcaacg cgaacgagga tcgctttgcg tccggcgctg
      601 cgcaattgct cggcctcttc ggcattgaaa acgatctcgc cgctggccgc gccgggcgag
      661 gccggcaagc ccgtcgcgat cacgtcgcgc tcagactcgg gcgctacatt cgggtgtagc
      721 aattggtcaa gcgagcccgg ctcgatgcgc agcaccgctt cctgttggct aatcagccct
      781 tcttgcgcca tctcgacggc ggctcgcagg gcggctttgg cggtgcgctt ggcgctacgc
      841 gtctgcaaca tccagagctt gccgcgctcg acggtgaatt cgacatcctg aatgtcgcga
      901 taatggcgct cgagctggtc ggcgacggca acgaactggt caaagacctc ggggagtgcc
      961 gtctccatcg acggctgctc cgagcctgcc gctatccgcg ccacctcggt gatattctgc
     1021 ggtgtgcgga cgccagctac gacatcctcg ccctgcgcat tgatcaggaa ttcgccgtaa
     1081 agctccttgg cgccaatcga tggattgcgg gtaaaggcga cgccggtcgc ggacgtgtcg
     1141 cccatattgc cgaagaccat cgcctgcact gtgacggcgg tgccccagga ttccgggata
     1201 ttgtgcaact gccggaagat cttcgcgcgc ggattgtgcc acgagccgaa gacggcggcg
     1261 atggcgcccc agagctgcga ttgtggctcc tgcggaaacg gcgaaccgtg cacctcggca
     1321 atcttcgtct tgaagatgga aatgattttt tgccaatccg ccgccgaaag atcggtatcg
     1381 agattgaggc tgttatcctc cttgaagatc tcgattgctt cttcaaaatg atgatgcgcg
     1441 acaccgagca cgacgctcga atacatctgg atgaagcggc ggtaggaatc gtaggcaaag
     1501 cgtgcgtcgc cggcatctct tgccaacgcc tcgaccgtcg cgtcgttgag gccgatgttc
     1561 agtacggtgt ccatcatgcc cggcattgac gcgcgcgagc cggagcgcac ggacacgagc
     1621 agcggccttt gcgcgtcccc gagctcgcgg ccggtgatcg cttcgatctg cttcagggcc
     1681 gcatcgactt gcggcttgag gttgctcgga taggttttcg cattggcgta aaaataagca
     1741 caaacgtcgg tcgtgatggt gaagcccggt ggcaccggca agccaagatt ggccatttcc
     1801 gccagattgg cgcccttgcc gcccagcaga gcatgcatct ggctgctgcc ctcggcggtg
     1861 ctgccgccga acgtatagac ccacttcgtc atcaaggtcc ccgaattggc cgcccgcacg
     1921 cgaacgatcc ataaagcagc gcgagcgccg acggaacgtt cttctgattt taagcgtgac
     1981 gcgggagagt gttaagatgg gctttcatcc gcaggccaag ggtgatccag cttccgcccc
     2041 agaaaagcaa atcaagccca agaagcacgc cgaggatagc cagactgttc gccggccagc
     2101 cgatcaggac gacgagacca acaagcgatg tcacgacgcc cgcgacaacg acgatggtgc
     2161 gcacatcgtt gttgagatgc gtgccgacat agatgcggat gagcccggtg acgaacatgg
     2221 caacgccgag aagcaaggtg aacaaagccg ccgccatcaa cggctgggcc agcgcgaagg
     2281 ccccaaccac aatataagcg aggccggcga tgacccagag aaagaaccga gaccaggagc
     2341 gcgagcagtt tgcgaaaaca atctcgccgc cgccggcgac gatcataaag aacgcaatga
     2401 tatagaccga gacgatggtc gcagcgacgg cctggaccaa ggcggcaacg cccattgtgg
     2461 cgacaagcgc gcccaagaca atgaacgagg gccagcgata acgcagacgc tcgacgcagc
//
LOCUS       DASWOG010000212         9319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_96748,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000212 DASWOG010000000
VERSION     DASWOG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96748"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..713)
                     /locus_tag="VGB93_04325"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="VGB93_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
     gene            complement(791..1390)
                     /locus_tag="VGB93_04330"
     CDS             complement(791..1390)
                     /locus_tag="VGB93_04330"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0145372.1"
                     /translation="MPNITLSDNYDSFTFNLVHYLGELGAEVDVHRNDKVSVAEVIAA
                     RPDAIVISPGPCTPHEAGICLDLIREASTDIPIFGVCLGHQAIGEVFGGDVVRAPIPV
                     HGKMSEISHRSETIFRGINGPFHATRYHSLVVKRETFPEELNVTAETDGLVMALSHKS
                     RPVHGVQFHPESIESEHGHRILANFLELAQEWNKAHRSA"
     gene            complement(1429..2433)
                     /locus_tag="VGB93_04335"
     CDS             complement(1429..2433)
                     /locus_tag="VGB93_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0145373.1"
                     /translation="MSQLETINIPGTDLQPTRVALGTWAIGGWMWGGSDDADSIRTIQ
                     SAVDRGINIIDTAPVYGFGHSEEIVGRALAEGGRREKTIIATKVALDWKDGKIFRNAS
                     KARIEKEIEDSLRRLQTDVIDIFQVHWPDDATPIAETAETLGKLFKAGKIRAIGVSNF
                     DVAQMEAFRAVAPLHTIQPPHNLFERSAEKDVLPYAAKNDIATLAYGPLCRGLLAGKM
                     NADSTFTGDDLRRNDPKFQPPRFAQYLAAVAALEAFAKNNYGKTVLDLAIRWVLDRQP
                     LSIALWGARKPSQLDPISRVSDWKLDSSAFAAIDKILTDNIKDPVGPEFMAPPVRKAA
                     "
     gene            complement(2561..4078)
                     /gene="trpE"
                     /locus_tag="VGB93_04340"
     CDS             complement(2561..4078)
                     /gene="trpE"
                     /locus_tag="VGB93_04340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY0145374.1"
                     /translation="MITPAYETFAATYDSGAAALLATRLVGDLETPVSAFLKLSVGRK
                     GRVFLLESVEGGGARGRYSMIGLDPDIVWRCQDGRAEINRTAATVPDAFTPCAGPPLE
                     ALRALIAESRIDAPNDLPPMAAGVFGYLGYDMVRQMEQLPPAKPDPIGVPDAIMLRPT
                     IMVVFDSVTDEIFVLTPVRPIADVAAKAAYERGIERIEAIVGALEAPLPPSATDLDAD
                     LQTANPTSNTSEARFHEMVENAKEYIRAGDIFQVVLSQRFTAPFTLPAFALYRALRRV
                     NPAPFLCYLDFGDFQIVCSSPEILVRARAGKVTIRPIAGTRWRGKTEEEDQRLSADLL
                     ADEKERAEHLMLLDLGRNDVGRVAKIGSVEVTERFAIERYSHVMHIVSNVEGELDAQH
                     DVIDALCAGFPAGTVSGAPKVRAMEIIDELETDKRGIYAGCIGYFGAHGDMDTCIILR
                     TSVVKDGQMHVQAGAGIVYDSDAAYERRETINKAQALFRAAEEAVRFAARTKRGQ"
     gene            complement(4083..6011)
                     /locus_tag="VGB93_04345"
     CDS             complement(4083..6011)
                     /locus_tag="VGB93_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0145375.1"
                     /translation="MLDALRASSQTWVGRSIMAVVMGLLVIAFGFWGIADVFRGFGAN
                     TLARVGSAEITPEQFQVAYQRELVQMQEQQRRAISSEEARERGIDRQVLSRMLSDAAL
                     DQEAHRLGLALPDSVIARQIMQDDAFKGPDGQFNRQTFEARLQDNGLTEKNYVAETRA
                     AALRQEIVATLTNSLAAPRAMLEIINRYYHETRNIDYIVLPKSAAGAIPAPSDAELKS
                     YFESHRDLYRKPEYRKVSLLLVSSTSLAGIESAAHPISEAEIKKRYDEVKDQRYTQAE
                     QRQIQQIIFPDEKTADAAASKLAGGETFDALVAERKLAPKDTDLGTVTEATLADKSVA
                     TAVFALPAGGVSKPVKTQFGWAILHVEKIIPKIVIPLVAVKTSLAQELAIFRAHKDLG
                     KIHDQIEDQRAGGKSVADAAKAVGQSARTIDEIDAQGNNKASKAVPDLGDDQKAFLKA
                     VFASDIGVDNDPITTKDGGGIWFDVLNIDPARNLTFDEVKSRVTQDWTADETSRRLVD
                     KAVDLTKKLDGGTTIAALATAQGNLPVKHVDKITRLDPQGLSSAILDEVFNRAVGKAG
                     SAEAQDGGRIIFSVASANVPPLDVKRYDFANVLDQMKTALNDDMTAQFVTNLETGLNT
                     RINQQVWQSISGAPADQQ"
     gene            6181..6933
                     /gene="tpiA"
                     /locus_tag="VGB93_04350"
     CDS             6181..6933
                     /gene="tpiA"
                     /locus_tag="VGB93_04350"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0145376.1"
                     /translation="MSEKPRPLIAGNWKMNGLTADLAEIAAIRDALGGAEGGPGIVIC
                     PPATLIAAAAQICAGSPIAIGGQDCHPERHGAYTGDIAADMLRDAGASYVIVGHSERR
                     RDHGESDVLVSAKAEAALRAGLKVILCVGETEAERAAGRALDVVARQLEGSLPSAPHG
                     DDLVVAYEPVWAIGTGVTPTPNDVAEMHSFLRQRLAALLPESSDVRLLYGGSVKPDNA
                     AELLHLPAVDGALIGGASLRAAEFLAIVAASR"
     gene            7070..7441
                     /gene="secG"
                     /locus_tag="VGB93_04355"
     CDS             7070..7441
                     /gene="secG"
                     /locus_tag="VGB93_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0145377.1"
                     /translation="MQTVLIVIHIMVVLALIATVLLQRSEGGALGIGGGGGFMTGRGQ
                     ANVLTRATSILAALFFATSLGLTLLANAERSHGSIFEGATPVGAPTSQGTVLQQLQKE
                     QQQRAQQPPGPPAQPEPPRSQ"
     gene            7599..9227
                     /locus_tag="VGB93_04360"
     CDS             7599..9227
                     /locus_tag="VGB93_04360"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0145378.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRAAVREDNITTGRIYQEIISKE
                     RRGDYLGATVQVIPHVTNEIKEFVLRGNEGADFVLVEIGGTVGDIESLPFLEAIRQLG
                     NDLPRQHAIYVHLTLMPYIPSAGELKTKPTQHSVKELRSIGIQPQIILCRTDREIPRE
                     ERRKIGLFCNVRESAVIEARDVASVYDVPRAYHAAGLDQEVLAAFGIEPVPKPDMSRW
                     NAVTERVHNPEGDVTIAIVGKYTGMKDAYKSLIEALAHGGMANRVRVHLDWIESEVFE
                     GSDPAPRLERVDGILVPGGFGQRGAEGKILAARFARERNVPYFGICFGMQMAVIEAAR
                     SLAGIADANSTEFGATSDPVVGLMTEWMRGNELQMRAAEGELGGTMRLGAYSAFLKPG
                     SKIAGIYGETEISERHRHRYEVNMAYREQLEEKGMVFAGLSPDGLLPETIELANHPWF
                     VGVQYQPDLKSRPFQPHPLFASFVAAAGEQSRLV"
BASE COUNT         1781 a         2745 c         2945 g         1848 t
ORIGIN      
        1 cgtcggtttc ggctgggata gggttgcggt tctcccatcc ggcggaaggt cgcgtaggcc
       61 gtaaagataa atgccaaggc cgatcaccat gcccacgccg gcggcggcga aaccgtagtg
      121 ccgtcccgct tcctcgccca acgtgccgca gacgagcggc gagaagaacg cgccgatatt
      181 gatgccgacg tagaaaatcg agaacgcccg gtcgcgccgc gcgtcccccg gcggataaag
      241 gccgccgacc tgtgtcgaga tgttcggctt gaatgcgcct gagcccagaa tgagcgccag
      301 caatgcaaag agaaagagat gttcgacggc catcatgaaa tggccgatcg ccatcaacac
      361 cgcgccgacg atcacggttc ggcgccggcc gagcagacgg tcggcgagga ggccgcccag
      421 gataggcgtg aaatagacga ggcggtgtag aagccgtaaa tctgcgaggc gaggggctgc
      481 agcgcgagcg ggccgaacag agattccaat ccatgacgga ccgcggccag ccccgcgaca
      541 tgctccgcat ggcccggcag tagcagatat tttgtcatgt agagaacgag gagcgcgcgc
      601 attccgtaat aggaaaagcg ctcccacatt tccgtcgcga agagaaaaga caggcctttc
      661 ggatgaccga aaagcttagc cggagccgcc ccgccggtgc gcggaaattc gatcggttcg
      721 tccaagcaga ggagccctac gcgacgcgtg aaagaaactc accgtagcat ttcagtttca
      781 ggggccagag tcaggcgctc cggtgcgcct tgttccattc ctgcgcgagt tcgagaaaat
      841 tcgccaggat gcggtggccg tgttcggact cgatactttc ggggtgaaat tgcacgccgt
      901 ggacaggccg cgatttgtgc gagagtgcca tgacgagacc atccgtttcg gccgtcacat
      961 tcagttcttc gggaaacgtc tcgcgtttga cgacaagcga atgatagcgc gtcgcgtgga
     1021 atggcccatt gatgccgcga aagatcgtct cggatcggtg gctgatctcc gacatcttgc
     1081 catgcactgg aatgggcgca cgtaccacgt cgccaccgaa gacttcgccg atggcctgat
     1141 ggccgagaca gacgccgaag atcgggatat cggtactggc ttcgcggatg agatcgaggc
     1201 agataccggc ctcatgcggc gtgcaggggc cgggcgaaat cacgatggcg tccggccgcg
     1261 cggcaatgac ctccgcgacg ctcaccttgt cgttgcgatg cacgtccacc tcggcgccca
     1321 attcgccgag gtaatgcaca aggttgaaag tgaagctgtc gtaattgtcg ctcagggtga
     1381 tgttgggcat agcagccttt tgagccgggt gggctggcag gtcaagactc aagccgcctt
     1441 gcggaccggc ggtgccatga attccggccc gaccggatcc ttgatgttgt cggtgagaat
     1501 cttgtcgatc gccgcgaatg ccgaggagtc gagcttccag tccgagacgc gggaaatcgg
     1561 gtcgagctgg cttggtttgc gggcgcccca gagagcgatg ctcagcggct gacggtccag
     1621 aacccagcga atggcgagat cgaggaccgt tttgccgtaa ttgttcttcg caaatgcctc
     1681 gagcgccgcg acggcggcca gatattgcgc gaaacgcggt ggctggaatt tggggtcgtt
     1741 acggcggaga tcgtcgccgg tgaaggtcga gtccgcgttc atcttgcctg cgagcaggcc
     1801 gcggcagagc gggccatagg cgagcgtcgc gatatcgttt ttggcggcat agggcagcac
     1861 atccttttcc gcactgcgct cgaagagatt gtgcggcggc tggatggtgt gcagcggcgc
     1921 gacagcgcga aaggcttcca tctgcgcaac gtcaaaattg ctgaccccga tggcgcggat
     1981 ttttccggcc ttgaacagct tgcccaaggt ctctgcggtt tcggcgattg gcgtcgcgtc
     2041 atccggccaa tgcacttgaa agatgtcgat cacatccgtc tggagacggc gcagcgaatc
     2101 ctcgatttct ttctcgatcc gcgctttgct ggcgttgcgg aagattttgc catctttcca
     2161 gtctagcgcg actttggtcg cgataatcgt cttctcacgg cggccacctt cggccaacgc
     2221 gcggccgacg atctcttccg aatggccaaa gccatagacg ggtgcggtat caatgatgtt
     2281 gatgccgcga tcgaccgcgc tctggatggt gcggatcgag tcggcatcgt cgctgccgcc
     2341 ccacatccag ccgccgatcg cccaggtgcc gagcgcgacc cgggtcggct gcaggtcggt
     2401 gccgggaata ttgatggttt caagctgaga catggccgat cctttgctga aagcctcagt
     2461 gtaatatcgg gctcagcacc atattttcga gtcggcgagc caaaaatgcg caagatcaaa
     2521 catgaaatgg cagtttctga gcgattcaac gaaatgcgaa ttactggccg cgcttggtcc
     2581 gcgccgcgaa gcgcacagct tcttccgccg cgcggaacaa ggcctgtgcc ttgttgatcg
     2641 tctcgcggcg ctcgtaggcc gcatcggaat catagacgat tcccgcaccg gcctggacgt
     2701 gcatctggcc gtctttcacc actgaggtgc gcaagatgat gcaggtatcc atatcgccgt
     2761 gcgcaccgaa atagccaatg cagccggcat aaatgccgcg cttatcggtt tcgagttcgt
     2821 caatgatttc catcgcccgc actttcggcg cgccggagac tgtgccggct ggaaagccgg
     2881 cgcataacgc atcgatgaca tcgtgctgcg catccagttc gccttcgaca ttcgagacga
     2941 tgtgcatcac atggctgtag cgctcgatgg cgaagcgttc cgttacttcg acactgccga
     3001 ttttcgcgac gcggccgaca tcgttgcggc cgagatcgag cagcatgaga tgttcggcgc
     3061 gctccttttc gtcggcgagg agatcggcgg aaagtctctg gtcctcctcc tcggtcttgc
     3121 cgcgccagcg cgtgccggcg atcggccgga tcgtcacttt gccagcgcgg gcgcgcacaa
     3181 gaatttccgg gcttgaacaa acgatctgaa aatcgccgaa gtccagataa caaaggaacg
     3241 gcgcggggtt cacgcgccgc agcgcacggt aaagcgcaaa cgcgggcagg gtgaatggcg
     3301 cggtgaatcg ttgcgacagc acgacctgaa agatgtcgcc agcgcgaatg tattctttcg
     3361 cgttctcgac catctcgtgg aagcgcgctt cggacgtatt ggatgtcggg ttcgcggtct
     3421 gaagatcggc gtccaaatcc gtggcgctcg gcggcagcgg cgcttcgagt gcaccgacaa
     3481 tcgcctcgat gcgttcgatg ccgcgctcat acgcggcctt ggcagcgaca tcggctatcg
     3541 gacgcaccgg cgtcaggaca aaaatctcgt cggtgacgct gtcgaagaca accatgattg
     3601 tcgggcgcag catgatcgcg tcgggcacgc cgattgggtc aggtttggcg ggcggaagtt
     3661 gctccatctg ccgcaccatg tcgtaaccca ggtagccgaa aacgccggcc gccatcggcg
     3721 gcagatcgtt gggcgcgtcg atgcgggatt cggcgataag cgcgcgcaaa gcctcaagtg
     3781 gcgggccggc gcagggggta aaggcgtcgg gcacggtcgc cgccgtccgg ttgatttccg
     3841 cgcggccatc ctggcagcgc cagacgatat ccggatcgag cccgatcatc gaatagcgtc
     3901 cgcgcgcgcc gccgccttcg accgattcca gcaggaagac gcggcccttg cggccgacag
     3961 acaatttgag aaaggcggag accggggttt cgagatcgcc gacgagccgc gtcgcgagca
     4021 gcgccgccgc gcccgaatcg taagtcgctg caaaagtctc gtaggccggc gtgatcatgc
     4081 ggtcactgct gatccgccgg agcgcctgaa atcgattgcc agacttgctg gttgatcctt
     4141 gtgttcaagc ccgtttcaag attggtgacg aattgcgccg tcatgtcgtc gttgagggcg
     4201 gtcttcattt gatcgaggac gttggcgaag tcgtaccgct taacgtccag cggtggcaca
     4261 ttggcgctcg cgacgctgaa gatgatgcgg ccaccatcct gcgcttcagc cgagccggct
     4321 tttccaacgg cgcgattgaa aacctcatca agtatcgcgc tcgatagacc ctggggatcc
     4381 agtcgtgtga ttttgtcgac atgtttgaca ggcagattgc cttgcgccgt tgcgagcgcc
     4441 gcgatcgtcg tgccgccgtc gagcttcttc gtcagatcga cagccttgtc gacgaggcgg
     4501 cgcgatgtct catcagcggt ccaatcctgc gtgacgcggc tcttgacctc atcgaacgtc
     4561 agattgcgcg ccggatcgat gttgagaacg tcgaaccaga ttccgccgcc atccttggtt
     4621 gtaattggat cattatcaac gccgatatcg gacgcgaaga cggccttcag aaaggctttc
     4681 tggtcatcac cgagatcagg caccgccttg ctcgctttgt tgttaccttg cgcatcgatc
     4741 tcatcgattg tgcgggccga ctgcccgaca gccttggccg catcggcgac ggacttaccg
     4801 cccgcgcgct gatcttcgat ctgatcatga atcttgccga ggtccttatg tgcgcggaag
     4861 atcgcaagct cctgggccag cgaagtcttc acggccacaa gcgggatgac aatcttcgga
     4921 atgatcttct cgacgtgcag gattgcccag ccgaattgcg tcttcactgg cttggagaca
     4981 ccaccggccg gcaaagcgaa gaccgcggtg gcgacgctct tgtcggcgag agttgcttcg
     5041 gtgacggtgc caaggtcggt atccttcggc gccaacttgc gttcggcaac cagcgcgtcg
     5101 aaggtctcgc cgccggcgag tttggaggcc gccgcgtcgg ccgtcttttc gtccgggaag
     5161 atgatctgct gaatctgccg ctgttcggcc tgcgtatagc gttgatcctt gacctcgtcg
     5221 tagcgcttct tgatctcagc ctcgctgatc ggatgggcgg cggactcgat gcccgctaaa
     5281 cttgtcgaac tcaccaaaag caggctgacc ttgcggtatt caggcttgcg atagagatcg
     5341 cgatgcgatt cgaaatagct cttgagttcg gcatctgacg gcgccggaat cgcgccggcc
     5401 gcggatttcg gcaggacgat gtaatcgata ttccgggtct cgtggtaata gcgattgatg
     5461 atctcaagca tcgcacgcgg cgccgcgagg ctgttggtca gcgtcgcaac aatttcctga
     5521 cgcagagccg cggcgcgagt ttcggcgaca taattctttt ctgtcaatcc gttgtcttgc
     5581 agacgcgcct cgaacgtttg ccgattgaat tgtccgtcgg ggcctttgaa ggcgtcgtcc
     5641 tgcatgatct ggcgtgcaat gacgctgtcg gggagggcga ggccgagacg gtgcgcctcc
     5701 tgatcaaggg ccgcgtcgct cagcatgcga ctcaggactt gccggtcgat cccgcgttcc
     5761 cgtgcttcct cgctgctgat cgcccggcgc tgctgttcct gcatctgaac gagctcacgc
     5821 tgataagcga cctggaattg ttccggcgtg atctcggccg atccgacgcg cgccaaagtg
     5881 ttggcgccga agcccctgaa aacgtctgcg atcccccaga agccgaaggc aatgacgagc
     5941 aatcccatca ccacggccat gattgagcgg ccaacccatg tctggctgga ggcacgcaac
     6001 gcatcaagca taatttagaa tctccgcttg cccagggggt cgattgggcg ccggactata
     6061 gggaggcgct ttcgggctgg caatgtatga agaatgactg ccgagcggcg cgcaaaacta
     6121 gccgattttg ccattccagc gacggatgtt agggcgtagg caaacgcagg acatttgagc
     6181 atgtcggaaa aaccgaggcc gctgatcgcc ggaaactgga agatgaacgg cctgactgcc
     6241 gacctcgccg agattgcggc gattcgcgat gcattgggcg gagccgaggg gggcccgggc
     6301 attgtcattt gtccgccggc gacactgatc gccgctgccg cgcaaatttg cgccggctcc
     6361 cccatcgcca tcggggggca ggattgccat cccgaaagac acggtgctta cacgggcgat
     6421 atcgcggcag acatgctgcg cgacgccggc gcttcctatg taattgtcgg ccattcggag
     6481 cgccgccgcg atcatgggga gagcgacgtg ctggtctcgg ccaaggctga agccgctctg
     6541 cgggccgggc ttaaagtcat tctctgcgtc ggcgagaccg aggctgagcg cgccgccggc
     6601 cgagctctcg acgtcgtcgc ccggcagctt gagggatcgc tgccctcggc gccgcatggc
     6661 gatgaccttg tggtcgctta cgagccggtc tgggcgatcg gcaccggcgt gacgccgacg
     6721 ccgaacgatg tcgccgagat gcacagcttt ttgcgtcaaa gactggcggc gctgctgccg
     6781 gaaagtagtg acgtgcgcct tctctacggt ggctcggtta aacccgataa tgccgcggaa
     6841 ttgctgcatc tgcctgcggt cgacggcgcg ctcattggcg gcgccagcct cagagccgcg
     6901 gaatttctgg ccatcgtggc ggcctctcgc tgagcgcaaa tggctgaatt cgcactgctt
     6961 tccttccggc tgccggaagc ggggcggaaa ttacgctagc cagacggaag gtcgcggtat
     7021 aagagcggcc gaacaaaatt tttgtcctgc cgataggctt ttcgcaccga tgcaaaccgt
     7081 tctgattgtc attcacatta tggtcgttct ggcgctgatc gcgaccgttc ttctgcagcg
     7141 ctcggagggc ggcgccctgg gcatcggcgg cggcggcggg ttcatgaccg gacgcggcca
     7201 agccaatgtg ctgacgcgcg cgacgtcgat tcttgcggcc ttgtttttcg cgacgagcct
     7261 agggctgaca ttgttggcga acgccgagcg gtcgcacggg tcgatttttg agggtgcgac
     7321 tccggttggc gcaccgactt cgcagggcac tgttctgcag cagcttcaaa aagagcaaca
     7381 gcaacgcgcc cagcagccgc cagggccgcc ggcccagccg gagccgccta ggtcgcaatg
     7441 acgtcgagat gagctgacgg cgctgaacgc cctttcaggg cgttcagcgc ccggtttttg
     7501 acaatcgcaa gacaaacccc gctgaaaacg ggttctgtgt ggatgggccg ataaaaaggc
     7561 tccccaaaca gttcaaaaca ggatattcac cgaggcccat ggcgcggtac attttcatca
     7621 ccggcggcgt ggtctcttcc ctcggtaaag ggcttgcatc agcggctctc ggggcattgc
     7681 ttcaggcacg cggctatacg gttcggcttc ggaagctcga tccctatctc aacgtcgatc
     7741 cggggacgat gtccccctat cagcacggcg aagtcttcgt cactgacgat ggcgcggaaa
     7801 ccgatctcga tctcggtcat tacgaacgat tcactggccg cgccgcagtg cgcgaagaca
     7861 acatcaccac cgggcgcatc tatcaggaga tcatctccaa ggagcggcgc ggcgactatc
     7921 tcggcgcgac ggtgcaggtg attccgcatg tgacgaacga gatcaaagaa tttgtcctgc
     7981 gcggcaacga gggcgcggat tttgtcctgg tcgaaatcgg cggcaccgtc ggcgacattg
     8041 agagcctgcc gtttctcgaa gcgattcgtc agctcggcaa cgatctaccg cgccagcatg
     8101 cgatctatgt ccatctgacg ctgatgccct acattccgag cgccggcgaa ttgaagacca
     8161 agccgacgca gcattcagtt aaagagctgc gctcgatcgg catccagccg cagatcatcc
     8221 tctgccggac ggatcgcgag attccgcgcg aggaacggcg caagatcggg cttttctgca
     8281 acgtgcgcga aagtgcggtc atcgaggccc gcgatgtggc gtccgtttac gacgtgccgc
     8341 gcgcctatca cgccgccggg ctcgaccagg aggtacttgc cgcctttggc atcgagccgg
     8401 tgccgaaacc cgatatgagc cgttggaatg ccgtgaccga gcgcgtgcac aatcccgaag
     8461 gcgacgtgac gatcgcgatc gtcggcaaat atacgggaat gaaggacgcc tataagtcgc
     8521 tgatcgaggc gctcgcgcat ggtggcatgg ccaatcgtgt cagggtgcat ctcgattgga
     8581 tcgagtccga agttttcgag gggtccgacc cggcgccgcg gcttgaacgc gtcgacggta
     8641 ttctggtgcc gggcggtttc ggccaacgcg gcgccgaagg caagattttg gcggcgcgct
     8701 tcgcgcgtga gcgcaatgtc ccttatttcg gcatttgctt cggtatgcag atggcggtga
     8761 tagaggcggc gcggtcgctg gcaggcattg ccgatgccaa ttcgaccgaa ttcggcgcga
     8821 cgagcgatcc tgtcgtcggg ctgatgacgg aatggatgcg cggcaacgaa ttgcagatgc
     8881 gcgctgcgga aggcgagctt ggcggcacga tgcggctcgg cgcctattcc gccttcctca
     8941 agccgggttc gaaaattgcc ggcatttacg gcgagacgga aatctccgag cgccatcgcc
     9001 accgctacga agtcaacatg gcctatcgcg aacagctcga agaaaagggc atggtcttcg
     9061 cgggcctgtc accggatggc ctgctgccgg aaacgatcga actcgcaaac catccctggt
     9121 ttgtcggcgt ccaatatcag cctgatttga aatcgcggcc cttccaaccg catccgctgt
     9181 ttgctagttt cgtcgccgcc gcgggggaac agagccggct cgtctgatca cgcctgcgat
     9241 tgcgcgacgt acgccgcgag gccttcgctt aggtgatcca taatcagccg caggcgcttg
     9301 ctcgtacgca gatcttcgt
//
LOCUS       DASWOG010000213         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_41231,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000213 DASWOG010000000
VERSION     DASWOG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41231"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VGB93_04365"
     CDS             <1..728
                     /locus_tag="VGB93_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEY0145379.1"
                     /translation="RALRRGIIIALLAATFQAIVAIPLVGLAAVVFNATAARMNAAAQ
                     YLEYAAYAGIIVLGLVLLFTKTTALVASIRHAWAGRALPVFAGAPQKLTYAQAGGSGN
                     FLADDGSSHIHGPDCGHFHAPDPAQLGVGFSWKSAAVTIVTAGARPCSGAILILVFSI
                     AQGLFGAGIFATFAMALGTAITTGALASFAVFAKKTAVRFSGRGSSRAEIIGRLIEVG
                     AALCVLVFGTLLLMASISGMTVA"
     gene            814..2130
                     /locus_tag="VGB93_04370"
     CDS             814..2130
                     /locus_tag="VGB93_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY0145380.1"
                     /translation="MVLWKARLLRRENKLANLICRSALLLAAALLLCLSSGASPAEAQ
                     FSPFNWFQQVFRPQRSYDYAPPRPHEHHAYRPRTVAKVVKPPAVPPTFFVAVLGDSLG
                     QLLGQGLTTALSDRPEVEVLRDAKEDSGLVRDDYYDWPKAAHDLVASGQKINVAVMMI
                     GSNDHQTLHDSTGSYDPGTPQYQQIYSARIEAVAKIFHDAKIPLLWVGLPVMKTDRFS
                     TEMAALNDMYRQYASRNGATYIDTWEDFVDDDGQFSSYGPDVDGQVVRLRALDGVHFT
                     KAGALKLASFVEPQIRDILNVSAPQDDAALAKIETTAPAGTNNPAAGAAVTPVKPPIG
                     PILPLTGPVLAPGGQLATLAPAAPKTDTASLVEQTFANGKPAPATPGRADDFAWPRKE
                     LAAAPPSQPAPAPVEGPPAPPKAPASAPGPVKIAPAKPTTAPGSVP"
     gene            2218..2832
                     /locus_tag="VGB93_04375"
     CDS             2218..2832
                     /locus_tag="VGB93_04375"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0145381.1"
                     /translation="MKFRTLALFVAIFSFGAGTALAQDLPFPFNLFSGASSQATRAQP
                     ASLPTDVAVNAPPTKTLVDYPTSASAGSITVDTKSKFLYLSLGDGKALRYGVGVGRDG
                     FGWTGHVHVARRAEWPDWTPPDAMLERRPDLPRYMPGGIGNPLGARALYLYNAKGDTG
                     FRIHGTNEPDTIGQAVSSGCIRMLNVDVEDLYTRVKVGTQVNVI"
     gene            2949..3167
                     /locus_tag="VGB93_04380"
     CDS             2949..3167
                     /locus_tag="VGB93_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0145382.1"
                     /translation="MADKVGDDERAALRADLERLRQEHRDLDSAIEGLAHVSTSDQLQ
                     IQRLKKRKLILRDKIDRIEDVLTPDIIA"
     gene            3282..>6076
                     /locus_tag="VGB93_04385"
     CDS             3282..>6076
                     /locus_tag="VGB93_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145383.1"
                     /translation="MSAIPQTATSLEFSESALLQSAIEFHSGKQYGIARQLYLQILEQ
                     NPDHELAWHNLGLVEHMTGRHAQAAEYIGKAVALKPDYARAYANLAAVLRETRQLETA
                     REMADHAIRLEPGFAPAHNNLGGILEDLGDMEAALAAYLEACRRDPFLIEAHTSAAEI
                     LRKLHRHEEGLKLCAAVSAKRPDAASPHFAAGNILRDLLRLDEACAEFRQAISLDPDF
                     AGAYCNLGNLLQQRNDFAGAVANYEKALAIKPDFTEIHCNLGMAYESLRRIDDALNSY
                     ARAVALDPSLVGVRLQLLHQRRAICDWAGDEAEEAAALAAAAASNGPVAPFGLLAMNS
                     GHALQLNVARRWAKAFRAKPSFEHRRDIPDRDRKLRIGYLSGDFFRHATAVLMAGLFE
                     HHDRAHFEIIAYSHGIDDGSELRHRLLKAFDRFVDITALDDRAAAARIYADEIDILVD
                     LKGYTQFSRNEIPAHRPAPIQVNFIGYPGTMGADFIDYVIADPVTLPMDQQPYYDEKI
                     VHLPDTYQPNDRLRPIDARTPTRAEAGLPDEGFVFCSFNNSYKLTPKFFDIWMRLLAA
                     VPGSVLWLLDANARVQANLRKEAEARGVSGDRLIFAPRFNLGAHLARHRLGDLFLDTL
                     PYNAHTTTSDALWAGLPVLTMQGDTFAGRVASSLLHAVGLPELVTHSLEDYEALALKL
                     ARTPELLADLRARLYASRLTAPAFDTVRFARHLEAAYSRMWETWADGKAAQPFAVAPL
                     ENTKLQSEARVPQIARHIYATCPLCGSGAHKVLLTADCGKDPAYRPELTPQVQWHVCE
                     DCDHVFADGYFAAGTFAPSAFGYDMEAGRRTVAPLVAAVARHIGARNCDAAWLDVGFG
                     SGAVLLTAAEFGFETVGLESSPVHVTALRQLGLEGHIDTIENFDAPVRFAVISLDDQL
                     PRLSDPAAALATAHRLL"
BASE COUNT         1116 a         1997 c         1777 g         1186 t
ORIGIN      
        1 aacgggcgct gcggcgcggc atcatcatcg ccctccttgc tgcgaccttt caggcgatcg
       61 tcgccatccc actcgtgggc ctcgctgcgg ttgtcttcaa tgcgacagcg gcgcggatga
      121 atgcggcggc ccaatatctc gaatatgccg cctacgccgg catcattgtt ctcggccttg
      181 tgctgctctt caccaagacg acggcgctgg tggcgagcat tcgccatgcc tgggcggggc
      241 gcgcgctgcc ggtttttgct ggtgcgccgc aaaagctcac ctatgcgcag gcgggcggca
      301 gcggcaattt ccttgcggat gacggaagca gccatatcca cggcccggac tgcggccatt
      361 tccatgcgcc ggatccggcc cagctcggcg tcggcttttc ctggaaatcc gccgctgtca
      421 cgatcgtgac agcgggcgcg cgaccttgct ccggcgccat attgatcctc gtcttttcga
      481 tcgcgcaggg cctgtttggc gccggaattt tcgccacctt tgccatggcg ctcggcaccg
      541 ccatcaccac cggggcgctc gccagcttcg cggtcttcgc caaaaagacc gcggtacgct
      601 tctcgggccg cggttcgtcc cgcgcagaaa tcatcggccg gttgatcgaa gtcggcgcgg
      661 cgctgtgcgt gcttgtcttc ggcactttat tgttgatggc gagcatctca ggcatgaccg
      721 tagcctgagc gccgcaagtc tttatcccct tgggtctttt ccccttaaac ttctgcgccg
      781 aagggcgtaa gttggagttt gaggcgaaag cggatggtgc tttggaaggc gcgtcttttg
      841 cggcgcgaaa acaagctcgc gaacctgatc tgccggtcgg cactcttgct ggccgccgcg
      901 ctcctgcttt gcctttcgag cggcgcaagt ccagccgaag cgcagttcag tcccttcaac
      961 tggtttcagc aggtcttccg cccccagcga agctatgatt atgcgccgcc gcggcctcat
     1021 gagcatcacg cctatcgacc gcgcaccgtc gccaaggttg tgaagccgcc ggcggtgccg
     1081 ccgacattct ttgtcgccgt ccttggcgat tcgctcggtc agcttctcgg ccaaggactc
     1141 acaactgcac taagcgacag acccgaggtc gaagttctgc gcgacgccaa agaagattcc
     1201 ggcctcgtgc gcgatgatta ttatgattgg ccgaaggcgg cacacgatct cgttgccagc
     1261 gggcagaaga tcaatgtggc cgtgatgatg atcggcagca acgatcacca gacgttgcac
     1321 gattcaaccg gcagctacga tcccggcacg ccgcaatatc agcaaatcta ttcggcgcgg
     1381 atcgaggcgg tcgcgaaaat cttccacgac gcgaagatac cgctgctctg ggtcggtctg
     1441 ccggtcatga agaccgatcg cttttccacg gaaatggcgg cgctcaacga catgtatcgc
     1501 caatatgcga gccgcaatgg cgcgacctat atcgacactt gggaagattt cgtcgacgac
     1561 gacgggcagt tcagttccta cggccccgac gtcgatggcc aggtggtgcg gctgcgtgcg
     1621 ctcgacggcg tccatttcac caaagctggc gcattgaagc tcgcgagttt cgttgagccg
     1681 caaatccgcg acatcctcaa cgtcagcgca ccgcaggacg atgccgccct cgccaaaatc
     1741 gagacgaccg ccccagccgg caccaataac ccggcggcag gcgcggcggt cacgccggtg
     1801 aagcctccca ttggtcccat cttgccgtta actgggccgg tgctcgcccc gggcggccag
     1861 ctcgcgacgt tggcgccggc cgcccccaaa acagataccg ccagtctcgt tgagcagacg
     1921 ttcgccaatg gcaaaccggc gccagcgacg cccggccgtg ccgacgattt cgcctggcca
     1981 cgaaaagagc tcgcagccgc gccaccgtcg caaccggctc ctgcgccggt tgaaggaccg
     2041 ccagctccgc cgaaggcgcc cgcctccgct cctggccccg tcaaaatcgc gccggcaaaa
     2101 cccacaacgg cgcctggcag cgtgccttaa gtttagcgcg gaacaaatcg cacatccttt
     2161 ggccggccag cgtggctcta tccgcacgcg gcgtttttct gtcgggggtg cgttatgctg
     2221 aaattccgga cactagccct ttttgttgca atattttctt ttggtgctgg cactgcgctg
     2281 gcgcaggatc tgcctttccc attcaatctg ttttccggcg ccagttcgca agcgacgcga
     2341 gcccagcctg cgagcctgcc aaccgacgtc gcggtcaatg cgccgccgac gaagactctg
     2401 gtcgattatc cgaccagcgc gtcggcgggt tcgatcaccg tcgatacgaa gtccaagttc
     2461 ctctatctct cgctcggcga cggcaaggcc ctgcgctacg gcgtcggcgt tggccgcgac
     2521 ggcttcggct ggaccgggca tgtccacgtc gcccggcgcg ccgagtggcc ggattggacg
     2581 ccgcccgatg caatgctgga gcgccgcccc gatctgccgc gttacatgcc cggcggcatc
     2641 ggcaatccgc tcggcgcccg cgcgctctac ctctacaatg cgaagggcga caccggcttc
     2701 cgcatccacg ggaccaatga gcccgatacg atcggccagg ccgtctcctc aggctgcatt
     2761 cgcatgttga atgtggatgt cgaagacctc tacacccgcg tcaaggttgg cacgcaggtc
     2821 aacgtgatct gatctcggtc tcctgcttgc ggaggatttg caggcagggc ctaaaatgct
     2881 ttatcccgat gacaatcggc accggccggc aatgatttgc cggccgcagc gtttcgggac
     2941 gggcggcaat ggcggacaag gtgggcgacg acgagcgcgc ggcgctacgc gcagatctcg
     3001 aacgtctgcg acaggaacat cgcgacctcg actccgcgat cgaggggctc gcccacgtct
     3061 cgacctccga ccaattgcaa atccagcggc tgaagaagcg caagcttatc ttgcgcgaca
     3121 agatcgaccg gatcgaggac gtgctcactc cggatatcat cgcctgatct cgaaggcccc
     3181 gtaagcccag ggcaaggccg cgcgtgtaga gcgcctaggc tacgctcaag aattgcgccc
     3241 ggcggtggcc ggacgcaccg aggccgccga tgaatcactc catgagtgcc ataccccaaa
     3301 ccgcgacgtc cttggaattc tccgaatccg cgctcctcca aagcgcaatc gaattccaca
     3361 gcggcaaaca atatggcatt gcccgtcagc tttatctgca aatccttgag cagaatcccg
     3421 atcacgaact tgcctggcac aatctcggcc tcgtcgagca catgaccggg cggcatgcgc
     3481 aagcggcgga atacatcggc aaagccgttg ctctcaaacc cgattatgcc cgcgcctacg
     3541 ccaacctcgc cgcggttcta cgcgaaacgc ggcaactcga aacggcacgc gaaatggcgg
     3601 atcacgccat acggctggag cccggcttcg ccccggccca caacaatctc ggcggcattc
     3661 tggaagacct gggtgatatg gaggcggcgc tcgccgctta tttagaagct tgtcggcgcg
     3721 acccgttttt aatcgaagcg catacaagtg ccgccgaaat cctccgtaag cttcaccgtc
     3781 acgaagaggg gctgaagctc tgcgccgcgg tatcagcgaa acggccggac gcggcaagcc
     3841 cgcatttcgc ggcgggcaac atcctgcgcg acctgcttcg cctggatgaa gcgtgcgcgg
     3901 agttccgaca ggccatttcg ctcgatcccg atttcgcggg agcctattgc aacctcggca
     3961 atctgttgca gcagcgtaat gatttcgccg gcgccgtggc caattatgaa aaggcgctcg
     4021 cgatcaagcc ggacttcacg gagattcatt gcaatctcgg gatggcttac gaatcgttgc
     4081 gacgcatcga cgatgcactg aattcttatg cccgcgctgt cgcgctcgac ccgtctctgg
     4141 tcggtgtgcg tttgcagctt ctgcatcaac gtcgcgccat ttgcgattgg gcgggcgacg
     4201 aggccgagga agctgcggcc ttggctgccg cggccgcaag taacggaccc gttgcgccct
     4261 tcggcctgct tgccatgaac tccggccatg cgctgcaatt gaacgtggcg cgacgctggg
     4321 caaaggcatt ccgcgccaag ccaagtttcg agcatcgccg ggatatcccg gatcgcgatc
     4381 gaaagttgcg gatcggctat ctctctggcg atttcttccg ccacgcgacg gcggttctca
     4441 tggccggact gttcgaacac cacgaccgcg cgcacttcga gatcatcgcc tattcgcatg
     4501 gtatcgatga cggcagcgag ctacgtcacc ggctgctcaa ggctttcgac cgtttcgtcg
     4561 atattaccgc actcgacgat cgtgccgcgg cggcacgcat ctatgctgac gaaatcgata
     4621 ttctggtcga tctcaagggc tatacacaat tttcgcgcaa cgagatccct gcgcatcggc
     4681 cagcgccgat ccaggtaaat ttcattggct atccaggcac gatgggtgcc gacttcatcg
     4741 actacgtgat tgccgatcca gtcactctgc caatggatca gcagccttat tacgacgaga
     4801 aaatcgtcca cctgccagat acctaccagc cgaatgacag gctccgtccg atcgatgcgc
     4861 gcacacccac gcgagccgaa gccgggcttc ccgatgaagg cttcgtcttt tgcagtttca
     4921 acaatagcta caagctcaca ccaaaattct tcgatatctg gatgcggctg ctggccgctg
     4981 ttcccggcag cgttctatgg cttcttgacg cgaacgcgcg cgtgcaggcc aatctccgca
     5041 aggaggcaga ggcgcgcggc gtcagcggag accggcttat attcgcgcca cgattcaacc
     5101 ttggtgcgca tctcgcgcgg catcgtcttg gcgatctctt tctcgatacc ctgccctaca
     5161 acgcgcacac gacgacgagc gatgcgctct gggccgggtt gccggtactt acaatgcagg
     5221 gcgacacgtt cgccggtcgc gtcgcttcga gccttctcca tgctgtcggc ttgcccgaac
     5281 tcgtcaccca ttcgctcgaa gattatgaag ccttggcgct gaagctcgcg cgcacgccgg
     5341 aacttctcgc agatttgcgt gctcgccttt atgccagtcg cctgacagca cccgccttcg
     5401 atacggtgcg ctttgcccgt catctcgaag cggcctattc gcggatgtgg gaaacgtggg
     5461 cggacggcaa ggccgcgcaa cccttcgccg tggcgccgct cgaaaataca aagctccaat
     5521 ccgaggcgcg ggttccgcag atcgcgcgcc acatctatgc gacatgcccg ctgtgcggca
     5581 gcggcgcgca caaagtcctt ctcacggctg attgcggcaa agacccggcc tatcgtcccg
     5641 aactgacgcc gcaggtccaa tggcatgttt gcgaagattg cgaccatgtc ttcgccgacg
     5701 gctattttgc cgccggcaca tttgcgccgt cagccttcgg ctacgacatg gaagcggggc
     5761 gccgcaccgt cgcgccgctc gtggccgccg tcgcacgcca catcggagcg cgcaattgcg
     5821 atgccgcctg gctcgatgtc ggcttcggca gcggcgccgt gctgctcacc gcggcggaat
     5881 tcggcttcga gacagtcggg cttgaaagca gcccggtgca tgtaaccgcc ctgcgccagc
     5941 tcggccttga gggacatatc gacacgatcg aaaatttcga tgcgccggtc cgtttcgccg
     6001 tcattagcct tgatgatcaa ctgccacggc tttccgatcc ggcggcggcg ttagccacgg
     6061 cgcaccggct tctgca
//
LOCUS       DASWOG010000214         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_279810,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000214 DASWOG010000000
VERSION     DASWOG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279810"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VGB93_04390"
     CDS             complement(<1..942)
                     /locus_tag="VGB93_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HEY0145384.1"
                     /translation="MKILAISVAIFGAAAALVAAKTGDMTTALLAATTLICAYTTYKS
                     QHISSYLKIFVAVFAVEVIAFGVFCLVEALHLWPASWADYEIPESLPLTVALFAIAVY
                     LISFIPLVSAMMRIADRYFDSRDTTTARIWPFPPFHGLERKVAAAMVVFLVLINQAEV
                     GINIRLSFFSRDWFNAIQTRDAKAFWYQLFTVFLVWAFLYIFAAVVEYVVTSTLVIRW
                     RRWLTSFYLDHWLDNHRHYRMSLAGSGADNPDQRISEDISRFIDGGDVGYGIYSFSIL
                     LISNLSSLVSFAILLWILSANFTLPGTHIAVPGFLFWV"
     gene            complement(1070..1420)
                     /locus_tag="VGB93_04395"
     CDS             complement(1070..1420)
                     /locus_tag="VGB93_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0145385.1"
                     /translation="MGQLPLNESEKKTATRADLLNAVYESCKGLSRAQARDIFEMTLE
                     EISTALVRGETVKLRSFGLFSVRAKRERIGRNPRTGIEVPIKPRRVLTFRPSPVLVAL
                     INGESADSAEELQD"
     gene            complement(1616..>2505)
                     /gene="groEL"
                     /locus_tag="VGB93_04400"
     CDS             complement(1616..>2505)
                     /gene="groEL"
                     /locus_tag="VGB93_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0145386.1"
                     /translation="EDVEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAIL
                     TGGQLISEYLGIKLENVTLQMLGRAKRIRIEKETTTIIDGAGSKPDIEARIAQIKAQI
                     ADTTSDYDREKLQERLAKLAGGVAVIRVGGSSEVEVKEKKDRVDDALNATRAAVEEGI
                     LPGGGVPLLRAIKVLDGIKTVNADQKAGVDIVRKAIQTPSRQIVDNSGADGAVIVGKL
                     LESTDYGYGYNAQTGEYGDLVKLGIIDPTKVVRTALQDAASVAGLLITTEAIITEQPK
                     KESAPAGGGGMGGMGGMDF"
BASE COUNT          577 a          718 c          722 g          488 t
ORIGIN      
        1 aacccagaaa aggaagcccg gcacggctat atgcgtcccc gggagggtga aattggccga
       61 aagtatccag agcagaatgg cgaaggagac gagcgaacta aggtttgaga tcaggagaat
      121 agaaaaggaa taaatcccat agccgacgtc accgccgtcg atgaagcggc tgatgtcttc
      181 cgagattcgc tgatccggat tgtcggcacc ggaccccgcg agtgacatgc ggtaatggcg
      241 atgattgtcg agccaatggt cgagatagaa tgaggtcagc caacgccgcc agcggataac
      301 caatgtcgag gtgacgacat attcgaccac cgccgcgaaa atataaagaa aagcccagac
      361 cagaaagaca gtgaacagct gataccagaa ggccttcgcg tcccgcgtct ggatcgcgtt
      421 gaaccaatcg cgcgagaaga acgaaaggcg gatgttgatg ccgacttccg cctggttgat
      481 cagaacaaga aaaaccacca tcgcggccgc gactttgcgc tccaggccat ggaaaggcgg
      541 aaacggccaa atccgcgccg tcgtcgtgtc gcgcgaatcg aagtaacgat ccgcgatccg
      601 catcatcgcg ctgacgagcg ggatgaagga tatcaagtaa accgcgatcg cgaaaagcgc
      661 gaccgtcagc ggcagacttt cgggaatttc gtaatccgcc caactcgccg gccagagatg
      721 caaagcctcg acgaggcaga acacaccgaa ggcgatgacc tcaaccgcga aaaccgccac
      781 gaaaattttc agataactcg agatatgttg cgacttgtac gtcgtgtagg cgcagatcag
      841 ggtcgtggcg gcgaggagag ctgtcgtcat atcgccagtc ttggccgcga ccagagcagc
      901 ggccgcgccg aaaatcgcga cagaaattgc gagaattttc atatcagcgt cgatatccag
      961 ggaatttagc gggatcaaac cacgcttttg cgcacaggcg caaatcgctg cctagttaca
     1021 ggtttgtaac atttagggca tatcgcggcc gcgtctcggc gggcgagcgc taatcctgca
     1081 attcctcggc agaatccgcg ctttcgccgt tgatcagcgc aaccagaact ggcgacggac
     1141 gaaatgtcag cacccgtcgc ggcttgatcg gcacctcgat cccggtgcgg ggattgcggc
     1201 cgatgcgctc acgcttggca cggacggaaa aaagtccaaa cgaacgaagc ttgaccgtct
     1261 caccacgtac cagcgcagtg gaaatttcct caagcgtcat ttcaaaaatg tcgcgcgcct
     1321 gggcacgaga gaggcctttg caggattcat acacagcatt caaaagatca gcccgcgtgg
     1381 ctgttttttt ttcggactca ttcagcggca actgacccac tttaccccct cctgcttagg
     1441 ctgatggagc gcaactcgca tgtaatgcat acgctttgcg acgcggtttt agccagtgat
     1501 tgcaacgcga aaaaatataa tttctctcgc cgcgagagaa agtgcgtagg tgcggtaaac
     1561 aaaaagcccc ggattaccgg ggcttttaag tgtcgtttcg cacaaatgcg agattttaga
     1621 agtccatgcc gcccatgccg cccatgccgc cgccgccggc cggagccgat tctttcttgg
     1681 gctgttcggt gatgatcgct tcggtggtga tcaggagacc cgcaacggaa gccgcatcct
     1741 gcaacgcagt gcgcacgacc ttggtcggat cgataatgcc gagcttgacg agatcgccat
     1801 attcgcctgt ctgcgcgtta tagccgtagc cataatcggt gctttcgagc agcttgccga
     1861 cgatcacagc gccatcggcg ccagaattgt cgacgatctg gcgagacggg gtctggatcg
     1921 ccttgcggac gatatcgaca ccggccttct gatcagcgtt gaccgtcttg atgccgtcga
     1981 gcaccttgat ggcgcgcaac aacggaacgc cgccacctgg aagaatgcct tcttcgacgg
     2041 ccgcgcgggt cgcgttgagc gcgtcgtcga cgcgatcctt cttttccttc acctcgactt
     2101 cgctcgaacc gccgacgcgg atgaccgcga cgccgcccgc gagcttggca agacgctcct
     2161 gcagcttctc gcgatcgtaa tcagatgtcg tgtcggcaat ctgcgccttg atctgagcaa
     2221 tacgcgcttc gatgtcgggc ttggaaccgg cgccgtcgat gatcgtcgtc gtttccttct
     2281 cgatgcggat gcgcttggca cggccaagca tctgcagcgt cacgttttcg agcttgatgc
     2341 caaggtattc ggagatgagc tgaccgccgg tcaggatagc gatgtcttcg agcatcgcct
     2401 tgcggcgatc accaaagccc ggcgccttga cggccgcgac cttgaggcca ccgcgcagct
     2461 tgttgacgac gagggtcgcg agcgcttcgc cttcgacgtc ttccg
//
LOCUS       DASWOG010000215         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_151420,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000215 DASWOG010000000
VERSION     DASWOG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151420"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGB93_04405"
     CDS             <1..533
                     /locus_tag="VGB93_04405"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0145387.1"
                     /translation="RISCTYPEKTIEIGASIATGGPCLTVVARGRSNLGTWYDVDVGA
                     ETLDRTTAGSWQIGTKLNLERSLKIGDELGGHIVTGHIDGVANILAVEPFDGMSRFVI
                     EAPAVLARFIAEKGSVALDGTSLTVNSVAGNEFSILLIPHTLAVTTWGARQTGDALNL
                     EVDLMARYAARLSETK"
     gene            671..1069
                     /locus_tag="VGB93_04410"
     CDS             671..1069
                     /locus_tag="VGB93_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0145388.1"
                     /translation="MHGTLNHLLVADRIWMRRFTDEGETTQRLDAILHESFDDLRAAC
                     VMEDARISGFIDGLDEEQLTRTISYRAISKPAQVEQRLDAALAHFFNHQTHHRGQVHC
                     LLTSLTGDGPVLDLAAYQRQIGLSRPQSFA"
     gene            1191..1685
                     /gene="ribH"
                     /locus_tag="VGB93_04415"
     CDS             1191..1685
                     /gene="ribH"
                     /locus_tag="VGB93_04415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0145389.1"
                     /translation="MAETQRPKQSKAEPSVEGARFLIVEAPYYEEIAANLLEGALTAL
                     KAVDARYICWQVPGALEIPIAAKIALDMARTAGSPFHGVIALGCVIRGETAHFDIVAG
                     ESARALMDLALAEKLPLGNGILTVENMEQAQVRAEPQRGDKGGEAARAALALYRLQVG
                     QGTL"
     gene            1685..2149
                     /gene="nusB"
                     /locus_tag="VGB93_04420"
     CDS             1685..2149
                     /gene="nusB"
                     /locus_tag="VGB93_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0145390.1"
                     /translation="MAIASQRSAARLAAVQALYQMEVSGKGLAETQTEFEAFWIGNEI
                     EGAQYEEAEIAFFRDILAGVLAEQGPIDRAVDKTLSDGWPLARVDAVMRAILRAGSYE
                     LLRRKDVPVRVVIKEYFDVGGAFFGADEAGMINAVLDALARQYRAEDFQARA"
     gene            complement(2341..2589)
                     /locus_tag="VGB93_04425"
     CDS             complement(2341..2589)
                     /locus_tag="VGB93_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0145391.1"
                     /translation="MFDTKPVPFAVRTPLVDLGNKDKLGWGVAIWFGCDDADALHDHL
                     VEHGTSIIFAPKDGPFGRYFAFRDPFGYTITPHTVAQS"
     gene            complement(2589..2711)
                     /locus_tag="VGB93_04430"
     CDS             complement(2589..2711)
                     /locus_tag="VGB93_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0145392.1"
                     /translation="MPHLIGPDFIGIQAEDLEAARTFYTEVVGLKAVANSPQAP"
     gene            2778..>3061
                     /locus_tag="VGB93_04435"
     CDS             2778..>3061
                     /locus_tag="VGB93_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0145393.1"
                     /translation="MSSQKVGSRMGDPYATIGFALKQAQQALRTRLDSSLREIGLTTP
                     QYAVLAFLEVEAGASNAALARRAFVTPQTMQAILVALERSGFIARTPHPE"
BASE COUNT          581 a          909 c          961 g          610 t
ORIGIN      
        1 tgcgcatatc ctgcacctat ccggagaaga caatcgagat cggcgcatcg atcgcgacgg
       61 gcggtccttg tctcaccgtt gttgcgcgtg gtcgaagcaa cctcgggacg tggtacgatg
      121 tcgatgtcgg cgccgaaacg ctcgaccgca cgacggcggg gtcgtggcag atcggcacca
      181 agctcaacct cgaacgttcg ctgaagatcg gcgatgaact cggcggccac atcgtcacgg
      241 gtcatatcga cggcgtggca aacattctcg cggtggagcc gttcgacggc atgagccgtt
      301 ttgtgatcga agcgcctgcc gtgctggcac ggttcatcgc cgaaaagggc tctgtcgcgc
      361 tcgacggcac gtcgctcacc gtgaacagcg tcgccggcaa cgaattctcc atcctgctca
      421 tcccgcacac attggcggtg acgacctggg gtgcgcgaca gacgggcgac gcgttgaacc
      481 ttgaagtcga tctcatggcg cgttacgccg cacgtctttc ggagacgaaa taagaatcgc
      541 ggagacgaaa taaggatcgt cgcatgctgg cgcatttgcg gatgttcgcg ggttacaata
      601 cctgggcgaa tgcactgcga agctcagcga tgccgattat cgcgccgatc gcggcgcttt
      661 cttcaaatcg attcacggca cactcaacca tctgctcgtt gccgaccgca tctggatgcg
      721 tcgtttcacc gacgaaggtg agacgacaca gcggctcgac gcgatcttgc atgaatcctt
      781 cgacgacttg cgcgcggcct gcgtgatgga agatgcacgc atttcgggtt ttatcgatgg
      841 cctcgatgag gagcagctga cgcgcacaat ctcctatcgc gcgatctcga agcccgcgca
      901 ggtcgagcaa aggctcgatg ccgcgctggc gcatttcttc aatcatcaga cgcaccatcg
      961 cggccaggtg cattgcttgc tgacaagtct gacaggcgac ggccccgtcc tcgatcttgc
     1021 ggcctatcaa cggcagattg gcctcagtcg gccccagtct ttcgcctaac cctttgcatt
     1081 cgttcaggat caaagcagat tggccgatgt ggcgtcatgt aagccgcagg cccgcgcttg
     1141 ccgcagcggc tgtggcttgt tagtcagccg cacgcgatca cgaggtggaa atggccgaaa
     1201 cgcagcgtcc caaacaatcc aaggccgagc cgagcgtcga gggcgcgcgt ttcttgatcg
     1261 tcgaggcgcc ctattatgag gagattgcgg cgaacctgct tgagggcgcg cttaccgcgc
     1321 tgaaggcggt cgatgcgcgc tatatctgct ggcaggtgcc gggcgcgctt gaaattccga
     1381 tcgcggcgaa aattgcactc gacatggcgc ggaccgccgg ctcaccattt catggtgtca
     1441 tcgcgctggg ctgcgtcatc cgcggcgaga cggcgcattt cgatatcgtg gccggcgaaa
     1501 gcgcccgcgc cctcatggac cttgcgctgg cggagaagct gccgctcggc aacggtattt
     1561 tgaccgtgga aaatatggaa caagcccagg tacgcgccga gccgcaacgc ggcgacaagg
     1621 gcggtgaggc tgctcgcgcc gcgcttgcgc tgtatcgctt gcaggtcggg caggggacgc
     1681 tctgatggca attgcctcac aacgctccgc cgcacggctc gccgccgtgc aagcgcttta
     1741 tcagatggaa gtctccggca agggcctcgc ggagacgcag accgaatttg aagccttctg
     1801 gatcggcaac gagatcgaag gcgcgcaata tgaagaggcg gagatcgcct tctttcgcga
     1861 tattcttgcc ggcgtgcttg ccgagcaggg cccgatcgac cgcgccgtcg acaagaccct
     1921 ttccgatggc tggccgctcg cgcgcgtcga tgcggtgatg cgcgctattc tgcgtgccgg
     1981 cagctatgaa ttgctaaggc gtaaggacgt gcctgtccgc gtcgtgatta aggaatattt
     2041 cgacgttggc ggggcttttt tcggcgccga cgaagcgggc atgatcaatg ccgtgctcga
     2101 cgcgctggcg cggcaatatc gcgccgagga ttttcaggcg cgggcctaga tcgtttttcg
     2161 gctcgggctt tttcgcgaca gacagatgac gcgcctggaa gcggccgtcg cgggtggatc
     2221 gctttagttg gctgccttgg gcgagttggg ctcagccctt gacgccgcca gcagatgcag
     2281 aacgcttgct ataccaatca gcgtgcaatt tgcagctgct catcaccggt cattccacgc
     2341 tcaagactgc gcgaccgtgt ggggtgtaat cgtgtagcca aacgggtcgc gaaacgcgaa
     2401 gtaacggcca aacggcccgt ctttcggcgc gaagatgatt gacgtcccgt gctcgacaag
     2461 gtggtcatgc agcgcgtcgg cgtcatcgca gccgaaccag atcgcgacgc cccagccaag
     2521 cttgtcctta tttcctaggt cgaccaaggg tgtgcggacg gcgaacggga ccggcttggt
     2581 gtcgaacact acggcgcctg gggactgtta gcgacggcct tcagcccaac cacttcagtg
     2641 tagaatgtcc gtgcagcctc gagatcttcg gcttgaatgc caatgaaatc gggtccgatc
     2701 agatggggca tggccgggct ttccttcgct acgcgagcct atagtatcag gaggctgata
     2761 tatgtaagga cccttatatg tcaagtcaga aggttggctc gcggatgggc gacccgtacg
     2821 cgacgatcgg ctttgcgttg aagcaggctc aacaggcgct acgcacccgc ttggactcta
     2881 gcttacggga gattgggcta accacgcccc agtatgcagt cctcgccttc ctggaggtcg
     2941 aggcgggtgc ttcgaacgcg gcgctcgcgc gccgcgcctt tgtgactcca cagacgatgc
     3001 aggcaatcct ggtcgcactt gagcgctcgg gctttatcgc gcggacaccc catcccgaac
     3061 a
//
LOCUS       DASWOG010000216         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_96865,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000216 DASWOG010000000
VERSION     DASWOG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96865"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGB93_04440"
     CDS             complement(<1..596)
                     /locus_tag="VGB93_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0145394.1"
                     /translation="MAKRPNLLIVQTLVALLSGQSFAYGGDAITPLPVREIAPGVYAH
                     QAPVTAMNAASGGDIANIGFIVGDDAVAIIDTGGAPIIGERLKKAIEAVTHKPIRYVI
                     NTHEHPDHIFGNAAFVGPGVTFVGHKNLPRAMAERGPSYLATWRRVMTPDLVNGIKLV
                     PPTLLVGTDKDVKLDLGHRMLDLHDWPPMQTDCDVTIF"
     gene            complement(606..944)
                     /locus_tag="VGB93_04445"
     CDS             complement(606..944)
                     /locus_tag="VGB93_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0145395.1"
                     /translation="MQDWAGYRQQLEKTLAGVARISPDILKGDHLLSNAAPTPRTLDE
                     KTRQLIALAVAVTTRCDGCIAFHTEAARKAGATKEELVEALGVAIALNEGAALVYSAR
                     AVDAFDSLAG"
     gene            1071..1634
                     /locus_tag="VGB93_04450"
     CDS             1071..1634
                     /locus_tag="VGB93_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0145396.1"
                     /translation="MSETTREKLLAEAEVLVRKRGWSGFSYADLAAAVGLRKASIHHH
                     FHVKEDLGIALIEAYGLRYDQALAQIIASSADGLARVEAYAKLYLHGLELEQGCLAAA
                     MAAELGTLPPRIRDGVAAFFQRHIAWLHQILAEGQINGTIGDDVDPDQSARMVVATLE
                     SALMIERWLGGAPGFEAALAALRKSLK"
BASE COUNT          319 a          494 c          516 g          335 t
ORIGIN      
        1 tcgaagatcg tgacatcgca gtcggtttgc atgggcggcc agtcatgcag atcaagcatc
       61 cggtggccaa gatcgagctt cacgtcctta tcggtgccga cgagcaatgt cggtggcacg
      121 agcttgatgc cgttcacgag atccggcgtc atcacgcgtc gccatgtcgc cagataggaa
      181 gggccgcgct ctgccatcgc gcgtgggaga tttttgtggc caacgaacgt cacgccgggg
      241 ccgacaaaag cggcattgcc gaaaatatga tcgggatgtt cgtgcgtgtt gatgacgtag
      301 cgaatgggct tatgcgtgac cgcctcgatc gcctttttca gccgctcgcc gatgatcggc
      361 gccccgcccg tatcgatgat tgcaaccgca tcgtccccga cgatgaagcc gatgttggcg
      421 atgtcgccgc cgctcgcggc attcatggcc gtcaccggcg cctgatgcgc atagacgccc
      481 ggcgcgattt cacggaccgg cagcggcgtg atcgcgtcgc cgccataggc gaaactctgg
      541 cccgaaagca gcgccaccag cgtctgcaca attagaaggt tgggacgctt cgccaagata
      601 cgcgcctatc cggcgaggct atcgaaggca tcgaccgcgc gagccgaata aacgagagcc
      661 gcgccttcat tgagcgcgat ggcgacgccc agcgcttcga cgagttcttc cttggttgcg
      721 ccagccttgc gtgcggcttc ggtatgaaaa gcgatgcaac catcgcagcg tgtcgtcacg
      781 gcgacagcca gcgcgatgag ctgccgcgtc ttttcgtcga gagtccgcgg cgtgggtgcg
      841 gcattggaaa gaagatgatc acccttgagg atgtcggggc ttatacgcgc cacgccagcg
      901 agcgttttct cgagctgttg gcgatagccc gcccaatctt gcatttgctg actcatggcg
      961 tctcctcttt gttgcctacc ttctagttgg tagtgtctat tcggtcaatg tctgtgattg
     1021 agaatccgta ctgacttgac ggtcctgcct atcagtaggt aggcaatcat atgtccgaaa
     1081 cgacgcgcga gaaattgctc gctgaagccg aggtgctggt gcggaaacgc ggctggtccg
     1141 gcttcagcta tgccgatctg gctgcggcgg tcggcctgcg gaaggcgagc attcaccacc
     1201 attttcacgt caaagaagac ctcggcatcg cgttgattga ggcctacggc ctgcgctacg
     1261 atcaggcgct agcgcagatc atagcgagca gcgccgacgg gctcgcccgc gtcgaagcct
     1321 acgccaagct ctacctgcac ggcctggaac tggagcaagg ctgtctcgcc gctgccatgg
     1381 cggcggagct cggcacccta ccgccacgca tccgcgacgg tgtcgcggcc ttttttcagc
     1441 gtcacatcgc ctggctgcat caaattcttg cggaagggca gatcaacggc acgatcggcg
     1501 atgatgtcga ccctgaccaa tcggcgcgta tggtcgtcgc aacgctggaa agcgccttga
     1561 tgattgagcg ctggcttggc ggcgcgcctg gattcgaggc ggcgctggcg gcactacgca
     1621 aaagtctgaa ataacaacaa tctcagcttg aggagcccga ggat
//
LOCUS       DASWOG010000217        10367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_133485,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000217 DASWOG010000000
VERSION     DASWOG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133485"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..538)
                     /locus_tag="VGB93_04455"
     CDS             complement(296..538)
                     /locus_tag="VGB93_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017993077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEY0145397.1"
                     /translation="MAANDHKQLLHLVFGGELVDLEGTEFRDLDAIDIVGVFPDYQSA
                     YKAWKARAQATVDNAHMRYFVAHLHRLLEPTEPPKS"
     gene            complement(606..1493)
                     /locus_tag="VGB93_04460"
     CDS             complement(606..1493)
                     /locus_tag="VGB93_04460"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0145398.1"
                     /translation="MPTLDRQVSSDKREASDMPRSQALDMESLSHDHSLVAGVIEAAR
                     AAGEIARSYFRPGEKTVAGIHKKADGSPVTEADLAANLLLEERLRQLIPEAGWLSEES
                     ADLPGRMGHDFVLVVDPIDGTRSFAAGDPVWAVSVALVHRQRPVIGVVHAPALDETYV
                     AVKDSGATLNGQAIAVSRHAQFDAATRVAGPLGFAQRLRNAGLDFELLPRIPSLAVRI
                     SRVAAGALDVALISANAQDWDIAACDLILTEAGGRLMNLRGRQPLYNRVHTLHGELVA
                     GPDVLLTHFRAAAGLAATS"
     gene            1815..2285
                     /locus_tag="VGB93_04465"
     CDS             1815..2285
                     /locus_tag="VGB93_04465"
                     /inference="COORDINATES: protein motif:HMM:NF039656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HEY0145399.1"
                     /translation="MPKLDRNPEACAYHEPDQRADGGTRTIRLSARGIRIDRALGGVK
                     MRLAVPIEAYRGVVLTREDKLQRRFYRLTLDHSDADLSITLSRATDIAALIGPWSDWA
                     RFFAKPALLDAKIGADPEVRLRRPMRRRSRVRGRATRQTALKVFSAEVELFSRE"
     gene            complement(2290..2796)
                     /locus_tag="VGB93_04470"
     CDS             complement(2290..2796)
                     /locus_tag="VGB93_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY0145400.1"
                     /translation="MNAVLSDDLSVAPERFREAMSRVGSAVHIVTTGGHAGSAGITAN
                     AVTSVTAEPPTMLFCLNKTSRAAPLLVQNGVFCINTLASAHQELSDIFAGRTEHALEE
                     RFAAASWGKLVTGAPVLTTALVAFDCRLIEAKEMSTHLILIGRVEAIDVAPKTEALLY
                     MHRSYKQI"
     gene            2910..4031
                     /locus_tag="VGB93_04475"
     CDS             2910..4031
                     /locus_tag="VGB93_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0145401.1"
                     /translation="MRPHFLLLTLFSILLALASPAQAEIKIALAAPLSGPDAVFGTQL
                     RNGAEQAAADINARGGVLGQKFIIVPFDDRGDLKQGISIANHLVNEKIFFVVGHFYSS
                     ITLAVSEIYANHSILEITPSATNPQITERGLDLIFRTCGRDDQQSAVAAKYLAAQTGK
                     KIAILFDNTAYGKGLGDDVRQRLAKAGVQDVLYAGIDKGSKDYGRLVGRIKAAATDLV
                     YWGGGDADAGLLLKEMRAEGVTAQLLGSDSLASDEFARAGDASVEGALMTFPVDPRRR
                     PQAAGVMREFRARDIDPEVFTLNAYAAVEVLAQAAKAAGTFDPAAIAKTIHSGRPFPT
                     VLGTLTYDTKGDVTTPDYAVYAWRRGYEDQLEYGELTAQ"
     gene            complement(4032..4763)
                     /locus_tag="VGB93_04480"
     CDS             complement(4032..4763)
                     /locus_tag="VGB93_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY0145402.1"
                     /translation="MANDVTLSAAAAAGLLSFLSPCVLPLVPPYLTFIAGTTIEDVAT
                     EKVGRARRDIFLAAVLFVAGFSTVFVALGATASFFGQALRSHIATLSLLAGFAIIAMG
                     LHFLGVFRLNLLYREKRLEVQKPLGLWGAYVMGLAFAFGWTPCIGPILAAILAIAASE
                     ATAARGAGLLAIYSLGLGIPFLLAAAALGPFLGFLKRFKAHFGAVERVVGVLLIVTGI
                     AFLTGTMQNLSFWLIQAFPGLAKIG"
     gene            4999..6438
                     /locus_tag="VGB93_04485"
     CDS             4999..6438
                     /locus_tag="VGB93_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0145403.1"
                     /translation="MRVLAAFFFNVLCNFAIGLLVAKFLGPAEYGRFALAFAVAVAVQ
                     SAFFDCLKLGATRFYSERVRTENPTLRATLDISFITIALSIGVASSIMFLSGAHFKLS
                     NGLIALALVTAVSNGVFDYNTALVRARFHDQLYTRLVLVKNTLALLLTGGGAFIFHSA
                     TMALAGSILSMSGAVITARAALRDHGTTRQHASLATAKLIFSYSLPIVCANVLYLSIP
                     LANRSIIATLYGFSETGQFSLAYDLGSKAIQAIGSTLDVVLFQIAVALHDQHGPSKAK
                     TQVARNMAIVIAVVLPACNGIWLTLPSVENVVVPVAYRGPFGQLLTLMMAGLFSSAIV
                     QYGINPIFQIAKRTAPLIAAAVIACLVDVVLIMVLPKNPDASSFAVAQTGAYIAGLGA
                     LVIMATFTKPQWPRLRDLVLATVATAAMAAALLPLREHNPGLLTLVEQVIAGALVYCF
                     FVYVFDIANLREITIARLRPILARLQASS"
     gene            complement(6446..7837)
                     /gene="pcaD"
                     /locus_tag="VGB93_04490"
     CDS             complement(6446..7837)
                     /gene="pcaD"
                     /locus_tag="VGB93_04490"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY0145404.1"
                     /translation="MTETIIDGDAFNIIVEGEADAPAILLVHSLGTNLHVFDRQVAAL
                     TPHFRLVRFDARGHGGSRVSEGPYSIDQLGKDALAVLDALKIDKAHFVGLSAGGLIGL
                     WLLRHAPERIERAVLANVAAQIGSADIWNERIRVVAANGVEALAPGLVENWFTRQYRE
                     QHPEVVERFEQALAAMSSEGYAGGAVAVRDSDFRSDLAHVRHTVLVIVGKHDPVTPPG
                     VGALVASAIPGAKTVTLEAAHLSPVEAAEAFNDAVLEFLLDAGIEPEVGALVGEAPPK
                     RPRKPRTPKPKPGPIAEPAHEVHAEPVAVEPPKPRSRAVPKPKAEEPAVPAPVARPAR
                     KSAAKKSAPKKSVAKKSAAKKTARKTVAKKRVVKKATVKKAAAKKAPVKKAVKKPTRK
                     QAAKKHTVHKKAAKKALHKAATKKHASKKVVKKAARKSVVKKLARKTGKRSAKKAIVR
                     KTLRRSPARRGRR"
     gene            complement(8065..10104)
                     /locus_tag="VGB93_04495"
     CDS             complement(8065..10104)
                     /locus_tag="VGB93_04495"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY0145405.1"
                     /translation="MDFRSIYSQGFARVAACTTTCRLADPGANAEAILALLAEVDAKA
                     AALAVFPELALSGYAIDDLLLQDTLHTAIAAAVERLVATSTDLLPLILVGAPLRYGGR
                     LYNCALAIHRGQLLGVVPKVHLPNYREFYERRHFASGHGMSGEIVIGDLRAPFGPDLL
                     FVAEDVPGLVVHAEICEDLWVPVPQSSLAALAGATVLANLSASNITIGKSETRQLLIK
                     SQSQRCLAAYLYSAAGKGESTTDLAWDGETTIAENGAILAESARFSDHAQFVVADVDL
                     DLIAQERLRQGTFDDNRRYVGVVPEDFRLIDFRLSPPAGDVGLERNVARFPFVPDDPA
                     RLAQDCYEAYNIQVSALEQRLIATKIEKLVIGVSGGLDSTHALIVAAKAMDRLGKPRS
                     NILAYTMPGFATSEATKANAFALMKALGVSSKELDITETARLMLTNIGHPYGSGENVY
                     DITFENVQAGLRTDYLFRLANLHGALVLGTGDLSELALGWATYGVGDQMSHYAVNAGV
                     PKTLIQYLIRWCIATDQFAAGVSTTLESILQTEISPELVPAGAGGAVQSTEAIIGPYG
                     LQDFNLFYVLRYGFRPSKIAFLSEAAWGDVAKGEWPQDFPEDRRHAYDLAQIRGWLEV
                     FIRRFFGFSQFKRSALPNGPKVVAGGSLSPRSDWRAPSDGNAKIWLDDLAKVPKR"
BASE COUNT         2154 a         3184 c         2995 g         2034 t
ORIGIN      
        1 gccgcaattt tcgccggcaa taaagcacca gcgggctcag cggcgcgaag gcggcgctgg
       61 cggcctgata aatgggcaaa ataggaaggg ttttgatcaa gacgtaaacg actgccaaga
      121 tgaaccaagc ggcgacgcca gcggccccta atgaacaggt agagcgcgcc gcaatcaagc
      181 gcccggaggc aaaccatgca tgtttgacag acgtttgcgg ccagagcttg cggaagtgcg
      241 aaattgttcg cgaaaaaagc ggtttctcgc gctttgagag ctgtgaagct tcagctcaac
      301 ttttcggcgg ctccgttggc tcaaggaggc gatgcaaatg cgccacgaaa tagcgcatgt
      361 gagcattgtc gacggtcgcc tgcgcacgcg ccttccaggc tttgtaagcc gactgataat
      421 cgggaaacac gccaacgata tcgatcgcgt cgagatcgcg aaattcggtg ccctccaaat
      481 cgacaagttc gccgccgaaa acgagatgaa gaagctgttt gtgatcgttt gccgccatga
      541 aatgcccgtc gatttgcgtg actttgacgc ctatgctcta gcaaaaaaca ggcaaggacc
      601 aaagcttatg acgtggcggc aaggccggcc gcggccctaa agtgcgttag caggacgtct
      661 ggcccggcga ccaattcgcc atgtaacgta tggacccggt tatagagcgg ctggcggccg
      721 cgcaaattca tcaaccgccc gcccgcctcg gtgaggatca gatcgcaggc cgcaatgtcc
      781 caatcctgcg cattggcgga aatgagggca acatccaacg cccctgcagc aacccttgag
      841 atgcgcaccg ccagcgaagg aattcgcggc aaaagttcaa aatcaagacc tgcattccgc
      901 aatcgctgtg caaagcccaa aggcccggca acccgcgtcg cggcgtcgaa ttgcgcatga
      961 cgcgagacgg caatcgcctg cccgttaagc gttgcgccgg aatccttgac ggcaacatag
     1021 gtttcgtcga gagccggcgc gtggacgacg ccgatgaccg gccgctgccg atggacgagc
     1081 gcgaccgaaa cagcccaaac cggatcgcca gcggcaaaac tccgcgttcc atcgatcgga
     1141 tcgacgacca gaacgaaatc gtggcccata cgtcccggca gatcggcgga ttcttcggag
     1201 agccagcccg cttcgggtat gagttggcgc agacgctcct caagaagaag gttcgcagca
     1261 agatcggctt cggtgaccgg cgagccatcc gctttcttgt gtatcccggc gacagttttc
     1321 tcgcctggcc ggaaataaga gcgggcaatt tcgccggcgg cgcgcgcggc ctcaatgacc
     1381 ccagcgacca gtgaatgatc gtgcgaaagg ctttccatat ccaaggcttg cgaccggggc
     1441 atatccgacg cctcacgttt gtcgctgctg acctgtctgt ccaatgtcgg catgttcatg
     1501 cccaacccct atccgggttg aacgcgcgcc gggcgccaaa cgcagccacg catccgatta
     1561 atttttgcga tgccgcaatc tttgcgtgaa aggcagcaaa attcaggccg atcacgcgcg
     1621 gattgaagca acccgtcatc ccagaatacg cgccgcgggt gagattttca ttaaaattgc
     1681 ggctttttcg ccaagaaccg gacaagcata aaatcataaa actccatgaa tatcatgtat
     1741 ttactccata tttcaggaag cttccggcaa actttttcca gagccgcagc gggcggcaat
     1801 ttggagaaga cgcgatgccg aagctcgatc gcaatcccga agcctgcgcc tatcatgagc
     1861 ctgatcaacg cgccgatggt ggaacgcgga ccattcgcct gagcgcgcgc ggcattcgca
     1921 tcgatcgcgc gctgggcggc gtcaaaatgc gccttgccgt gccgatcgaa gcctatcgcg
     1981 gtgtcgtcct gacgcgtgaa gacaagcttc agcgccgatt ttaccgcctg acccttgatc
     2041 attccgacgc cgatctctca atcaccctgt ctcgcgcgac cgatatcgca gctctgatcg
     2101 gaccctggag cgattgggcg cggtttttcg ctaaaccggc attgctcgac gccaagatcg
     2161 gtgccgaccc ggaggtacgt ctgcgccggc cgatgcgccg ccgttcgcgt gtgcgtggcc
     2221 gcgccacgcg ccaaaccgcg ctcaaggtct tctcggcgga agttgagctc ttctctcgcg
     2281 aatagacgct cagatctgct tgtaactgcg atgcatataa agcagcgcct cggtcttcgg
     2341 cgcgacgtcg atggcttcga cgcggccaat caggatcagg tgcgtcgaca tttccttggc
     2401 ctcgatcaac cggcagtcga aagccaccag cgccgtcgta agcacgggcg cgccggtgac
     2461 aagcttgccc caggaggcgg cggcgaaacg ctcctcgagc gcgtgctccg tgcgcccggc
     2521 gaaaatgtcg gacagttcct gatgcgcgct cgccagcgtg ttaatgcaga aaaccccgtt
     2581 ctggaccagg agcggcgccg cccgcgacgt tttgttgaga caaaatagca tcgtcggcgg
     2641 ttcggccgtc actgaggtca cggcattggc cgtgatgcct gccgagcctg cgtggccccc
     2701 ggtggtcacg atatgaacag ccgagcccac gcggctcatg gcctcccgaa agcgttccgg
     2761 ggcgaccgaa aggtcatcgc tcagcactgc attcataaaa acgcattccc tgcaccaaaa
     2821 gccctctccc gatatcgcag acagggacgc cagcgtgaaa tagatagtag gatcagaccg
     2881 acaaacaaag ccttgggtgg acagcggcgg tgcggcccca ttttcttctt ttgacgctct
     2941 tttcaattct cttagcgctc gccagcccgg cgcaggctga gatcaagatc gccctcgcgg
     3001 cgcccttgag cggcccggat gcggtcttcg gcacgcaact gcgcaatggc gccgaacagg
     3061 cggctgcaga cattaacgcc agaggcggcg ttttgggcca gaaattcatc atcgtcccgt
     3121 tcgatgatcg cggcgatctc aaacagggaa tatcgatcgc caatcacctc gtgaatgaga
     3181 agattttttt cgttgtgggg catttttatt cgagcatcac actcgcggtg tcggagatct
     3241 acgccaacca ttccattctt gaaatcacgc cttcggcgac aaacccacag atcactgagc
     3301 gcggcctcga tctcatcttc cggacctgcg gccgggacga tcaacaaagc gcggtcgccg
     3361 cgaaatatct cgccgcgcag acgggcaaaa aaatcgcaat cctgttcgac aatacagcct
     3421 atggcaaagg actcggggac gatgtccggc agcggcttgc caaagccggt gttcaggatg
     3481 tgctttacgc gggcatcgac aaaggcagca aggattacgg acggcttgtc ggacgcatca
     3541 aggcggcggc aaccgatttg gtttattggg gcggcggcga tgcggacgcc ggtcttctcc
     3601 tcaaggaaat gcgcgcggag ggcgtaacgg cgcagctttt aggcagcgat tccctggcat
     3661 cggacgaatt cgcgcgagcc ggggacgcct cagtcgaagg cgcgctgatg accttccccg
     3721 tcgatccgcg ccgccgcccg caagcagcgg gcgtgatgag agaattccgc gcgcgcgaca
     3781 tcgatcccga ggtttttacg ctcaacgctt acgccgccgt cgaagttttg gcccaggcag
     3841 cgaaagccgc cggcacgttc gacccggccg cgatcgcaaa gacgatccat tccggccgac
     3901 cgtttccaac cgttctcggg acgctcacct atgataccaa gggagatgtc accacgccgg
     3961 attatgctgt ctatgcgtgg cggcgcggct acgaagacca gctcgaatat ggcgaactga
     4021 cggcccaata atcagccgat tttggcgaga ccgggaaaag cctgaatcag ccaaaaggat
     4081 aggttctgca ttgtgccggt gaggaaggcg atgcctgtta caatcagcag cacgccgacg
     4141 acgcgctcga cggcgccgaa atgtgccttg aaccttttca aaaagcccag gaacggcccg
     4201 agtgccgccg cggcgagtaa aaaaggaatg ccaaggccga gcgaatagat cgccaaaagg
     4261 ccggcgccac gtgcagctgt cgcttccgac gcggcgatcg ccaagatggc ggcgagaatc
     4321 ggcccaatgc acggcgtcca gccgaaagcg aacgcaagcc ccatgacata agcgccccag
     4381 agccctagcg gcttttggac ctcgaggcgt ttttcccgat acagaaggtt gagacgaaag
     4441 accccgagga aatgcagccc catcgcaatg atcgcgaaac cggcgagcag cgacagggtc
     4501 gcaatatgag agcgcaacgc ctgtccgaaa aacgaggcgg ttgcgccaag cgccacgaag
     4561 acggtcgaga aaccggcaac gaacaggacc gcggcgagga aaatatcgcg ccgggcgcgg
     4621 ccgacttttt cggtcgccac gtcctcgata gtcgtaccgg cgatgaaggt cagataaggg
     4681 ggaacgagcg gtaggacgca ggggctgaga aaggacaaga gcccggccgc tgcggcggcg
     4741 gaaagcgtga cgtcatttgc cattgggcgt tcctagcgcc aaagccgtgc ctaagaaagc
     4801 agtctttatc aaccctctat gaagctataa ggcgggggat ttgggaggaa tgcctttggc
     4861 gtccggccct gcccgcaaca cattcgcaag aaaaattgat tacaaagcag cgacccaaat
     4921 gaccacgccg cgccctcatc ctccccgcgc cagttgcttc accgaagccc cgaccacgcc
     4981 cgcccatgag cccccggcat gagggtcctt gctgcgtttt tcttcaatgt tctctgcaat
     5041 tttgcgattg gccttctggt cgcgaagttc ctcgggcccg ccgaatatgg ccgttttgca
     5101 ttggccttcg ccgtcgccgt cgcagtgcag agcgcgtttt ttgattgtct gaagctcggc
     5161 gcgacacgtt tctattccga acgcgtacgc accgaaaatc cgaccctgcg cgcgacgctc
     5221 gacatcagct tcatcacgat tgccctgtcg attggcgtgg cgtcgagcat tatgtttctc
     5281 tccggcgcgc attttaagct ttcgaacggg ctgatcgcat tggcgctggt cacagcggtc
     5341 tcgaacggcg ttttcgatta caacaccgcg ctcgtgcgcg cgcgattcca cgaccagctc
     5401 tacacccggc ttgtcttggt gaagaatacc ttggccttgc tgctgacggg gggtggtgcc
     5461 ttcatctttc actctgccac catggcgctg gcaggcagca tcctcagcat gtccggcgcc
     5521 gtcattaccg cgcgcgcagc gctgcgcgat catggcacga cacgccagca tgcgagtctt
     5581 gcgaccgcca aattgatctt ttcttattcg ctgccgatcg tttgcgccaa cgtgctctat
     5641 ctgtcgatcc cgctggccaa tcgctcgatc atcgcgactc tctatggctt ttccgaaacc
     5701 ggccaatttt cgctcgccta cgaccttggc agcaaggcga ttcaggcgat cggctcgacc
     5761 ctcgacgtgg ttctgtttca gattgccgtc gctctgcacg atcaacacgg gccgagcaag
     5821 gcgaagaccc aggtggcgcg caatatggcg atcgtcattg ccgttgtcct accggcctgc
     5881 aacggcattt ggctgacgtt gccgtccgtc gaaaatgtcg tcgtcccggt cgcctatcgt
     5941 ggaccattcg gccaattgtt gacgctcatg atggccggcc tcttcagttc cgccatcgtg
     6001 caatatggca tcaatccgat cttccagatt gccaagcgca ccgctccgct catcgctgcg
     6061 gcggtgatcg cctgcctcgt cgacgttgtg ctgatcatgg ttctgccaaa gaacccggat
     6121 gcttcgagct ttgccgtcgc ccagaccggc gcctacatcg ccggtctggg cgcgcttgtc
     6181 atcatggcga cctttaccaa gccgcaatgg ccgcgcctgc gcgatcttgt cctcgctacg
     6241 gtggcgacgg ccgccatggc cgccgcgctc ttgccgctac gcgagcacaa tcccggatta
     6301 ttgacgcttg tcgagcaggt cattgccggg gccctggtct attgtttctt cgtctatgtg
     6361 ttcgacatcg ccaatctgcg cgagatcacc atcgcccgat tgaggccgat cctcgcgcgg
     6421 ctgcaagcgt cttcctgacg gcttctcacc gccgtccgcg acgcgccgga ctccggcgaa
     6481 gcgttttcct gacgatcgct ttcttcgccg atcgcttgcc cgtcttacgc gccagtttct
     6541 tgactacgct tttgcgagcc gcctttttca ctaccttctt ggacgcgtgt tttttcgttg
     6601 ccgctttgtg cagtgctttt ttagccgcct ttttatggac cgtatgcttt ttggcagcct
     6661 gtttgcgcgt cggctttttg accgccttct tcacgggagc cttctttgct gcggccttct
     6721 tgaccgtagc ctttttcacg acgcgttttt ttgcaacggt tttgcgcgcc gtttttttcg
     6781 cggcagattt cttcgctacc gatttcttgg gcgctgattt cttggccgcc gatttgcgcg
     6841 cgggtcgtgc aactggcgcc ggaaccgctg gttcttcagc cttcggtttc ggcacggcac
     6901 gggatcgcgg cttcggtggt tcgaccgcga caggttcggc atgaacttcg tgcgccggct
     6961 ccgcgattgg ccccggcttc ggctttggcg tgcgaggttt gcgtggccgt ttcggcggcg
     7021 cttcgcctac cagcgctccg acttcgggct ctatgcccgc atcgagcaaa aattcgagga
     7081 cggcatcatt gaacgcctcg gcggcttcga ccggcgacag atgcgccgct tccagagtca
     7141 cagtcttcgc cccgggaata gcgctcgcaa ccaaagcacc cacccccggt ggcgtcaccg
     7201 gatcatgctt gccaacgatg acgagaaccg tatggcgaac atgggcgagg tcggagcgga
     7261 aatcagaatc ccgtacggcc acggcgccgc ccgcataccc ctcggagctc atcgccgcca
     7321 gcgcctgttc aaaacgctcg acgacctcgg ggtgctgctc gcgatattgc cgggtaaacc
     7381 aattctcgac gagacccggc gccaacgctt cgacgccgtt tgctgccaca acgcggatgc
     7441 gttcgttcca gatatccgcg ctgccgatct gcgccgcgac attggcgaga acggcacgct
     7501 cgatccgctc cggcgcatga cgcagcagcc acagcccgat aagaccgccc gccgaaaggc
     7561 cgacgaaatg cgccttgtcg atcttcagcg catcgagaac cgcgagggca tccttgccga
     7621 gctggtcgat cgaatagggc ccctcgctga cgcgactgcc gccatgcccc cgcgcatcga
     7681 agcgaacgag ccgaaagtgc ggcgtcaggg ccgcgacttg ccgatcgaaa acatgaagat
     7741 tggtgcccaa cgaatgcacg agcaagatcg ccggggcgtc cgcttcgccc tcgacaatga
     7801 tattgaaggc gtcgccgtcg atgatggttt ctgtcattgc cactctctcc cgctcacgcc
     7861 gcgttcaggc ccgatggtca acagcgcaaa cgtaaactag cttgctcgtg aaacgccgca
     7921 agggactgcg acgtagataa tcaccgcgtt gatgcaaaag ttgtccgcgt ctgcgcccgt
     7981 cgagaacgca cggcaccacg ccgcgcggcg gcgttatgcg aaatatattt ttaaaatcaa
     8041 atggttgaac ggataaagtc ctgtctaacg cttcggcact ttagcgagat cgtccaacca
     8101 gattttagcg ttgccatcgg atggcgcgcg ccagtcgctg cgcggcgaca gcgatccacc
     8161 cgcgacgacc ttcggcccgt tcggcaatgc cgagcgcttg aattgactga agccgaagaa
     8221 acgacggatg aagacctcaa gccagccgcg aatctgcgcg agatcgtagg catggcggcg
     8281 atcttctgga aaatcctgcg gccattcgcc tttggcgaca tcgccccacg cggcctcaga
     8341 cagaaatgcg atctttgagg gccggaagcc atagcgcaag acatagaaaa gattgaagtc
     8401 ctgaagccca taggggccga taatcgcctc cgtgctttgc acggcgccgc ccgcgccagc
     8461 ggggacgagt tcgggcgaaa tttccgtctg caaaatggat tcaagcgtcg tcgaaacgcc
     8521 ggccgcaaac tggtccgtcg cgatgcacca gcggatcaga tactggatca aggtcttggg
     8581 gacgccggca ttgacggcgt aatgcgacat ctggtcaccc acaccatagg tggcccaacc
     8641 aagcgcgagt tccgagaggt cgcccgtgcc gaggaccagc gcgccgtgca gattggcgag
     8701 acggaaaaga taatccgtcc gcaagccagc ctgcacgttc tcgaaagtga tgtcgtagac
     8761 attctcgccg ctgccgtacg ggtggccgat gttggtcagc atcagccgcg cggtctcggt
     8821 gatatcgagt tctttgctgc tgacgccaag tgccttcatc agggcgaagg cattggcttt
     8881 ggttgcttcg ctcgtggcga acccaggcat cgtataagca agaatgttgc tgcgcggctt
     8941 gccgagccga tccatcgcct tcgccgcgac gatcaacgca tgtgttgagt cgaggccgcc
     9001 cgagacgccg atgacaagct tttcaatctt cgttgcgatg agacgctgct cgagcgcgct
     9061 cacctgaatg ttataggctt cgtagcaatc ctgcgccaaa cgcgccgggt cgtccggaac
     9121 gaacggaaaa cgcgcaacat tgcgttccag tccaacgtcg cccgccggcg gcgaaagacg
     9181 aaaatcgatc agccggaaat cttccggcac gacgccgaca tagcggcggt tatcgtcgaa
     9241 cgtgccctgc cgcagccgct cctgcgcgat gaggtcaaga tcaacgtcgg cgaccacgaa
     9301 ttgcgcatga tcggagaagc gcgccgattc ggcgaggatc gcgccatttt cggcgatggt
     9361 tgtttcgcca tcccaagcaa gatcggtcgt cgattcgccc ttcccggccg cggaataaag
     9421 ataggccgcg aggcagcgct gcgattgcga cttgatgagc aattgccgtg tttcgctctt
     9481 gccgatcgtg atgttgctcg ccgaaagatt ggcgaggacc gtcgcgcctg ccagggcggc
     9541 aagtgacgat tgcggcaccg gcacccaaag gtcctcgcaa atttcggcat gaacaacgag
     9601 gccgggcaca tcctctgcga caaacaaaag atccggcccg aacggtgcgc gaagatcgcc
     9661 gatcacgatc tcgccgctca tcccatggcc cgacgcaaaa tgccggcgct cataaaactc
     9721 gcgataattc ggcaggtgaa ccttcgggac gacaccgagc aactggccgc gatgaatggc
     9781 gagcgcgcaa ttatagagcc gacctccata acgcagcggt gcgccgacaa gtatcagcgg
     9841 cagaagatca gttgaagttg ccacgaggcg ttcgaccgca gcggcaatgg ccgtatgcaa
     9901 tgtatcctgc agcagcaagt cgtcgatcgc atagccggac aaggcaagct cgggaaaaac
     9961 cgcgagtgcg gccgccttcg catcgacctc agccagcaaa gcgagaattg cctccgcatt
    10021 ggcacctggg tcggccagac ggcacgtcgt cgtgcaggct gcgacgcgtg caaaaccctg
    10081 actatagatc gagcggaaat ccatcaatat cattcgtctc gtgcgaggcc ggcgcaacgc
    10141 ggcaatctag gtaacaagat aaatcattct cgcgctcgaa ccattcccat ttaaatgcgt
    10201 tgctttcggt tcgcgtaaac gcagttaatt gtgacagtgt ttccgcgcac agccgctctg
    10261 aagaattcat ctagcgttca cgcggcaagt cgcagattca agattatgcg gttgtcttcg
    10321 cagtgcaaac tcgactacgg aaccaaccgc gctttgatgc aatggaa
//
LOCUS       DASWOG010000218         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_96937,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000218 DASWOG010000000
VERSION     DASWOG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96937"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1278
                     /locus_tag="VGB93_04500"
     CDS             337..1278
                     /locus_tag="VGB93_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0145406.1"
                     /translation="MASPDDAVLLDAYSRTVSEVVERVGPAVVRIDIRRGDGKHGGSG
                     SGVIVSPDGLILTNSHVVQGGKRAEITTLDGRNFSARVLGDDPDTDLALIRVDADTTL
                     PFAALGDSKTLKRGEIAIAIGNPLGFDATVTAGIVSALGRSLRAKSGRMIDDVIQTDA
                     ALNPGNSGGPLVSSSGEVIGINTAMIVGAQGICFAVAANTARFVVGELIRHGRVRRAA
                     LGIGGGTIPLPRRIALRLGLEQATGAVITAVEQGGPADEAGLLTGDIVLAVSGTPVTG
                     VDDLVRLLDADKIGSTVPLDVLRRSDKRRYWVALRER"
     gene            complement(1322..1678)
                     /locus_tag="VGB93_04505"
     CDS             complement(1322..1678)
                     /locus_tag="VGB93_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0145407.1"
                     /translation="MADTATRPIVEDVSLKDLKAGLADGSIVLVDVREPHEFAAGHIP
                     GSSLNPLQSFDPDALPPAEPGKRIVFSCRSGRRSITALGLAQQAGRNDIRAHYPGGIL
                     GWEGAGERVETFEDTI"
     gene            1892..2803
                     /locus_tag="VGB93_04510"
     CDS             1892..2803
                     /locus_tag="VGB93_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0145408.1"
                     /translation="MHRPYYLLDVFTDQPLAGNQLAVVIDSDGLDAATMQKIAAEFNL
                     SETVFIFPPRDPINTARLRIFTPKAELPFAGHPTIGAAVLIGELRAASLMRSEDVRIV
                     LEEPVGDVICHARHRPGSARQADFFLPKLPVLIGEAQPKAKIAAALGLTPEDIGFDRH
                     QPAIFSAGTPFTCVPIASCAALAAARPRLDLFAEAFAPSEVAHAYLYALPNDDASHDF
                     ETRMFAPTLGVLEDPATGSAAAAFAGALMAFENVPDGHHALRLEQGVALGRPSKIVLS
                     LDVEDGRLIMASIGGSAVIVADGTLNL"
     gene            2800..>3458
                     /locus_tag="VGB93_04515"
     CDS             2800..>3458
                     /locus_tag="VGB93_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0145409.1"
                     /translation="MSMPHIEKIAAIDIRLRSRIWDFATDRAAETEDYWQKRLQANPH
                     LYNGNVLLAADVVRDAMPAGVCLRGTCFVVEYKTFLAWRAFGQPGDTRNLFALAALRS
                     ADGAYLLSEMAAWTSNAGQIYSPGGTPDLHDIIDGALDLEGNALRELAEETGLSTQDV
                     SPTPGWTIIECGGLICCMKELRSPLTAAELVARAADFFAREEKPELTRLVPAFSPADI
                     NA"
BASE COUNT          616 a         1109 c         1057 g          676 t
ORIGIN      
        1 acgcggctgc ggcgcgtata ttcggcaaga aaccagcggc cgcgcacggt ctcgacaacg
       61 gccgcttcga tggcatcgta atcatccgcg ctcaagccct cgggcggcgg aatcatcgcc
      121 atcgtcttct cctgcctgcg aatcgctgcg ccgcagtctg cgccgggcgc cggcgaaatc
      181 caagccgcgc ggacggccgg atggggtctt ttacaggaca aaggcgtacc tacatctatg
      241 tccaacgaaa ggacttcaat gttcgacaat ccccacacat tcctcttgga cgacacgccg
      301 gaaagcgccg agacggtcgc ggccgttgcg ccgcccatgg catcgcccga cgatgcggtc
      361 ctcctcgacg cctattcgcg cacggtctcc gaggtcgtgg agcgcgtcgg cccggcggtc
      421 gtgcggatcg acatccgccg cggcgacggc aagcacggcg gctcgggctc cggcgtcatc
      481 gtctcccccg atgggctgat cctgaccaac agtcatgtcg tgcagggcgg caaacgcgcc
      541 gagatcacca cgctcgacgg acgtaatttc tccgcccgcg tgctcggcga cgatcccgat
      601 accgacctcg cgctcatccg cgtcgatgcc gataccaccc tgcccttcgc ggcgcttggc
      661 gattccaaaa cgctgaagcg cggcgagatc gccatcgcca tcggcaatcc gctcggcttc
      721 gatgcgacag tgacggctgg catcgtctcg gcgctcggcc gttcgctacg cgcgaaatcg
      781 ggccgcatga tcgacgacgt gatccagacc gatgcggcgt tgaaccccgg caattccggc
      841 ggcccgctcg tctcgtccag cggcgaggtc atcggcatca acacggcgat gatcgtcggg
      901 gcgcaaggca tctgtttcgc cgtcgcggcg aatacggcgc gtttcgtcgt cggcgaactt
      961 atccgccacg gccgcgtgcg ccgcgctgcg cttggcatcg gcggcggcac gatcccgctg
     1021 ccgcgtcgga ttgcgctgcg ccttggcctc gaacaggcga caggcgcggt gatcaccgcc
     1081 gtcgaacagg gtggccccgc cgatgaagcg ggcctgctga cgggcgacat cgtgctcgcg
     1141 gtttccggta cgccggtgac cggcgtggat gacctcgtcc ggctactgga cgctgacaaa
     1201 atcggctcga ccgtgccgct cgacgtcctg cgtcggtcgg acaagcggcg ctattgggtg
     1261 gcgctcaggg agaggtaata aaaggggcgc ttcggcgccc ctttcgctgg gtctgcatca
     1321 cctatattgt gtcttcgaag gtctccaccc tctcgcctgc gccttcccat cccaaaatcc
     1381 caccaggata atgcgcgcga atgtcgttgc ggccggcttg ctgcgcgagg ccgagtgcag
     1441 tgattgagcg gcgcccggaa cggcaggaaa acacaattcg tttaccgggc tctgccggcg
     1501 gcagcgcgtc ggggtcgaag ctctgcagcg ggttcagcga cgaacccgga atgtggccgg
     1561 cggcaaattc atgcggttcg cgcacatcga caaggacgat agatccgtcc gcaagccctg
     1621 ctttcaaatc cttgaggctc acgtcctcga cgattggtct tgttgccgta tcggccatta
     1681 tggttatcct gggttgtggg cacgcccggc gggccacgat tgtttaattc acgtcttttt
     1741 attgtatata tctacaatct acgttccaga ttagaataat atgctgcacg gcccgcgcaa
     1801 gcaagtcgtg agcaattttt atccgtggcg caaagttcaa tagacgcgct gccgatggcc
     1861 cgacacaaca catgggtcag gagagcacca tttgcatcgc ccctattatc tgcttgatgt
     1921 tttcaccgac cagccgcttg ccggcaatca gctcgctgtg gtgatcgaca gcgacggcct
     1981 cgatgcggca acgatgcaaa agatcgctgc cgagttcaat ctttccgaaa cggtcttcat
     2041 ctttccaccg cgcgatccga tcaacactgc gcgattgcgc atctttacgc cgaaagccga
     2101 actgcccttt gccggccatc cgacaatcgg cgcggccgtg ctcatcggcg aattgcgcgc
     2161 cgcgtcgctg atgcgcagcg aggacgtgcg catcgtgctt gaagagccgg tgggcgatgt
     2221 gatctgccac gcgcgccatc gtccgggctc ggcaagacag gcggatttct ttctgccgaa
     2281 actgccggtg ttgatcggcg aggcgcaacc aaaagcgaaa attgccgccg cgctcggcct
     2341 gacgccggaa gacatcggct tcgaccgcca tcagccggcg atcttctcgg ccggtacgcc
     2401 tttcacatgc gtgccgatcg catcctgcgc cgcgctcgca gcggcacgac cccggcttga
     2461 tctattcgcc gaggccttcg cgccgagcga agtcgcgcat gcctatctgt atgcgctgcc
     2521 caacgacgat gcgtcacacg atttcgaaac gcgaatgttt gcaccgacgc ttggcgtcct
     2581 tgaagatccc gcgacgggtt cggccgcagc agcttttgcc ggtgcgctga tggccttcga
     2641 aaatgtgccg gacggccatc atgccttacg gctggaacaa ggtgtcgcgc ttgggcggcc
     2701 gagtaaaata gtgctctccc tcgacgttga agacggtcgt ctgatcatgg cgtcgatcgg
     2761 tggttccgcg gtgatcgttg ccgatgggac gctcaatctt tgagcatgcc acatatcgaa
     2821 aagatcgccg caatcgacat tcgcctgcga tcgagaattt gggattttgc gacagatcgc
     2881 gccgccgaaa ccgaagacta ttggcaaaag cgcctgcagg ccaatccgca tctctataat
     2941 ggcaatgtgc tgctcgcggc cgatgtcgtt cgcgatgcaa tgcctgccgg cgtgtgcctc
     3001 cggggcacct gtttcgtcgt cgagtacaag acgtttctcg cgtggcgtgc gttcggtcag
     3061 ccgggggaca cgcgaaatct tttcgcattg gcggcactgc gctcggccga cggcgcgtat
     3121 cttctcagcg aaatggcggc gtggacgtcg aacgccgggc agatctattc tcccggcgga
     3181 acgcccgacc tgcacgatat catcgacggc gctctcgacc tcgaaggcaa cgcgttgcgt
     3241 gagcttgccg aggaaaccgg cctttcaacg caagatgtgt cgccgacccc gggatggacg
     3301 attattgagt gcggcggctt gatttgctgc atgaaggaat tgcgctcgcc gttgaccgcc
     3361 gccgaactcg tggcgcgcgc ggcggacttt ttcgcgcgcg aggaaaagcc ggaactgacg
     3421 cgcctcgtgc cggcgttcag ccccgccgac atcaatgc
//
LOCUS       DASWOG010000219         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_5233, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000219 DASWOG010000000
VERSION     DASWOG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5233"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /gene="nadE"
                     /locus_tag="VGB93_04520"
     CDS             <1..334
                     /gene="nadE"
                     /locus_tag="VGB93_04520"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044117.1"
                     /note="catalyzes the formation of nicotinamide adenine
                     dinucleotide (NAD) from nicotinic acid adenine
                     dinucleotide (NAAD) using either ammonia or glutamine as
                     the amide donor and ATP; ammonia-utilizing enzymes include
                     the ones from Bacillus and Escherichia coli while
                     glutamine-utilizing enzymes include the Mycobacterial one;
                     forms homodimers; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY0145410.1"
                     /translation="FNLFYVLRYGIRPSKIAFLSAAAWGDVAKGEWPQDFPEDRRHAY
                     DLAQIRGWLEVFIRRFFGFSQFKRSALPNGPKVVAGGSLSPRSDWRAPSDGNAKIWLD
                     DLGKVPKH"
     gene            543..>1747
                     /gene="pcaD"
                     /locus_tag="VGB93_04525"
     CDS             543..>1747
                     /gene="pcaD"
                     /locus_tag="VGB93_04525"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY0145411.1"
                     /translation="MTETIIDGDAFNIIVEGDADAPAILLVHSLGTNLHVFDRQVAAL
                     TPHFRLVRFDARGHGGSRVSEGPYSIDQLGKDALAVLDALKIDKAHFVGLSAGGLIGL
                     WLLRHAPERIERAVLANVAAQIGSADIWNERIRTVAANGVEALAPGLVENWFTRQYRE
                     QHPEVIERFEQTLAAMSSEGYAGGAVAVRDADFRSGLAEVRHPVLVVVGKHDSVTPPG
                     VGALVAGAIPGAKTVTLEAAHLSPVEAAEAFNDAVLEFLLDAGIEPEVGALASEAPPK
                     RPRKPRTPKPKPAPVAEPTHEVHAEPVAVEPPKPRPRAAPKPKAEEPAVPASVARPAR
                     KSAAKKSAAKKSAAKKTTRKAVAKKRVVKKAAVKKVAAKKAGVKKAVKKPTRKQAAKK
                     HTAGKKVAK"
BASE COUNT          348 a          515 c          557 g          327 t
ORIGIN      
        1 cttcaatctt ttctacgtct tgcgctatgg gatccggccc tcaaagatcg cattcctgtc
       61 tgcggccgcg tggggcgatg tcgccaaagg cgaatggccg caggattttc cagaagatcg
      121 ccgccatgct tacgatctcg cgcagattcg cggctggctt gaggtcttca tccgtcgttt
      181 cttcggcttc agtcagttca aacgctccgc attgccgaac ggaccgaagg tcgttgcggg
      241 tggctcgcta tcgccgcgca gcgactggcg cgcgccgtcc gatggcaacg cgaaaatctg
      301 gctggacgat ctcggcaaag tgccgaagca ttagatcaat ctgatttaat tggcgtattt
      361 tgcgtagcct ccgccgtggt ctcgacggcc atgcgcttcc gccggtgcag agggggagat
      421 ttttcgcatc aacgcagtga ttatctacgt cacaatctct tgcggcgttt cacgagcaag
      481 ctagtttacg ttcgcgtcgt tgttcatcgg gcctgacacg gcgcgagcgg gagagagcgg
      541 caatgacaga aaccatcatc gacggcgatg ccttcaatat cattgtcgag ggcgacgcgg
      601 acgccccggc gatcttgctg gtgcattcgc tgggcaccaa tcttcatgtt ttcgatcggc
      661 aagtcgcggc cctgacaccg cacttccggc ttgtccgctt cgatgcgcgg ggccatggcg
      721 gcagtcgcgt cagcgagggg ccctattcga tcgatcagct cggcaaggac gccctcgcgg
      781 tcctcgatgc gttgaagatc gataaggcgc attttgtcgg tctatcggcg ggcggcctta
      841 tcgggctgtg gctgctgcgt catgcgccgg agcggatcga acgtgctgtt ctcgccaatg
      901 tcgcagcgca gatcggcagc gcggatatct ggaacgagcg catccgcacg gtggcggcaa
      961 atggcgtcga agccctcgcg ccgggtctcg tcgagaattg gttcacccgg caatatcgcg
     1021 agcagcatcc cgaagtgatc gaacgttttg aacagacgct ggcggcgatg agctccgagg
     1081 gttatgcggg cggcgccgtg gctgtgcggg atgccgattt ccgctctggg ctcgccgagg
     1141 ttcgccatcc agttcttgtg gtcgttggca agcatgattc ggtgacgccg ccaggcgtcg
     1201 gcgctttggt tgcgggcgct attcccgggg cgaagactgt gactctggaa gcggcgcatc
     1261 tgtcgccggt cgaagccgcc gaggcgttca acgatgccgt cctcgaattt ttgctcgatg
     1321 cgggcataga acccgaagtc ggagcgctgg cgagcgaagc gccgccgaaa cggccgcgca
     1381 aaccccgcac gccaaagccg aagcccgcgc cagtcgcgga gccgacgcac gaagttcatg
     1441 ccgagcctgt cgcggtcgag ccgccgaagc cgcggccccg cgccgcgcca aaaccaaagg
     1501 ctgaagaacc ggccgttccg gcctcggttg cacggcctgc acgcaaatca gcggccaaga
     1561 aatcagcggc caagaaatct gcggcgaaaa aaacgacgcg caaagccgtt gcgaaaaaac
     1621 gcgtcgtgaa aaaggcggcg gtcaagaagg tcgcggcgaa gaaagccgga gtgaagaagg
     1681 cggtcaaaaa accgacgcgt aaacaggctg ccaaaaagca cacggccggc aaaaaggtcg
     1741 caaaaaa
//
LOCUS       DASWOG010000220         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_151560,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000220 DASWOG010000000
VERSION     DASWOG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151560"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGB93_04530"
     CDS             <1..808
                     /locus_tag="VGB93_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145412.1"
                     /translation="PIASIEPITPAAVEAASQEAISSDPVAASIESGLEDAASTPDVV
                     SDEALAAPRDEATAAAAPAPVEAIEAESLASVIAEPVVEEVPVVEAAADPELAETATS
                     SEPAETAAAAETIESTTAEVSAEPATIEIWHPFRHRQVRRPARPQRQGQAQGQGRFQR
                     PAQIAPEAPAGASAVSAPDAAAAANQNPRPRPEHRRRHNFDGIPNGAPRPDRKRDFSQ
                     KDTHRGPRSGDGQAKGKWSSAEKPRERQPDPNSPFAKLLELKQRMEEGKN"
     gene            967..1242
                     /locus_tag="VGB93_04535"
     CDS             967..1242
                     /locus_tag="VGB93_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0145413.1"
                     /translation="MDTKSSEQKDELINQIVDVIVSEGMIDREKVKMDASIESLGLKS
                     IDIVMILTALEEKFDVYIPMDGPFHEAKDVKSLIDAMANYISKEKTA"
     gene            1243..2460
                     /locus_tag="VGB93_04540"
     CDS             1243..2460
                     /locus_tag="VGB93_04540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0145414.1"
                     /translation="MAQRRVVITGIGAISSLGIGAKALWEGARDGRSGVHETTFRHPY
                     PGRIKIAAQVQDFDATLYLDKSTLSLCDPVIKYLLVAADEAVQQAGLSRETLQGPRTA
                     SIIGTGIAGMNTIEDGLYTVFKREVRPDPLTIPRLIPSSCPAMVSMRYGTTGPSFAVG
                     SACSSATQAIGIGVQMIRAGMIDRAIVGGTEDCVTIGTMLAWEALRVLTPNFCRPFSK
                     DRNGMVIGAGAGVFILEAEEIAAERNAYVLAELAGYGTTSDAKDPVRPDVFGAASCMR
                     NAIEDAGVTPDEIDYVNAHGTGTTVNDLTESAAIGVVFGKRGPHVPVSSTKPIHGHGL
                     GVGGAFELIVTLGALRENVAPPTINWQVADEKCPVDCIPNEARPMPINVAMSNSFAFG
                     GINATLVVRRLAA"
     gene            2484..2870
                     /locus_tag="VGB93_04545"
     CDS             2484..2870
                     /locus_tag="VGB93_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145415.1"
                     /translation="MSGERALGTFVVRAAAAEAAAFRRETGGTGQGVPFTFPVRWLAR
                     ADFHAVAVDMIGDGNWVPIHESQSFDYRTPLASDTDYEMAVTMKREIEPSRIVLVAQI
                     GHPNDAPHLTMEMILRIIPMPTEQAA"
BASE COUNT          586 a          860 c          946 g          515 t
ORIGIN      
        1 tccgatagct tcgatcgagc ccatcacgcc cgctgccgtg gaggcggcgt cgcaagaggc
       61 aatttcatcc gaccccgtcg cggcaagcat cgaaagcggt ttggaggacg cggcttcgac
      121 tccagatgtt gtttcggacg aggccctagc ggcgcccaga gacgaggcga ctgctgcggc
      181 tgcacccgcc ccggtcgaag cgattgaggc cgaaagccta gcgtcggtca ttgcggaacc
      241 agtcgtggag gaggtgccgg tcgtcgaggc tgcagcggac ccggaactcg ccgagacagc
      301 gacgagctca gaacccgctg aaacggcggc tgcggcggag actatcgaaa gcaccaccgc
      361 cgaagtctcg gccgagcccg cgacgatcga aatctggcat ccgttccggc atcgccaggt
      421 tcggcgtccg gcgcggccac aacgacaagg tcaggcgcag gggcaaggcc gctttcaacg
      481 gccggcgcag atcgcacccg aggcgccagc aggcgcctcg gccgtttcag cgccggatgc
      541 agcggcagcg gcgaatcaga atccgcgccc gcggccggaa catcgccgcc gtcacaattt
      601 cgatggcatc ccgaacggcg cgccacgccc ggaccggaag cgcgatttct cgcaaaagga
      661 cacccatcgc gggccacgca gtggcgacgg ccaggctaag ggcaaatggt catccgccga
      721 aaagccgcgc gaacgccagc ccgatcccaa ttcacccttc gccaagctgc ttgaattgaa
      781 gcagcgtatg gaagagggaa agaattgaag tgggtttaaa ttccgcaatc tgacgtgtga
      841 cggtggctgg gcgagggggc gacataaggg cttccccggc acggcacccc cgtgttatcc
      901 cgacgggatt gacttaagct attggtctga cttccgcgca gacgttctcg gaaaagggga
      961 ttatcggtgg acacaaagag ttccgagcag aaggacgaac tcatcaacca gatcgttgat
     1021 gtgatcgttt cggaagggat gatcgatcgc gaaaaggtca agatggacgc ctcgatcgag
     1081 agcttgggac tgaagtccat cgatatcgtc atgatcctga cggcgctcga ggaaaaattc
     1141 gacgtctaca tcccgatgga cggcccgttc cacgaagcca aggatgtcaa aagcctgatc
     1201 gacgcgatgg cgaattacat cagcaaagaa aagactgcct gaatggcgca aaggcgggtc
     1261 gttatcacag gtataggcgc gatttcgtcg ttagggattg gcgccaaggc actctgggag
     1321 ggagcacgtg acggccgctc gggcgtgcat gaaaccacct tccgccatcc gtatccgggt
     1381 cgcatcaaga ttgcggcgca ggttcaggat ttcgatgcca cgctctatct cgacaagagc
     1441 acgctgtcgc tttgtgatcc ggtgatcaaa tacctgctcg tcgcggccga tgaggcggtc
     1501 cagcaggcgg gcctttctcg cgaaacgctg caagggccgc gcaccgcttc gatcatcggc
     1561 accggcattg ccggaatgaa tacgattgag gacgggctct acacggtctt taaaagggaa
     1621 gtacggccgg acccgctgac gatcccacgg ctcattccaa gttcatgtcc cgccatggtc
     1681 agcatgcgtt acggcacgac agggccgtcg tttgcggttg gcagcgcctg ttcgtcggca
     1741 acgcaggcta ttgggatcgg cgtgcagatg atccgcgccg gcatgatcga ccgtgccatt
     1801 gtcggtggca cggaggattg cgtcacgatc ggcacgatgc tggcgtggga agccttgcgg
     1861 gtgctgacgc cgaatttctg ccggccgttc tcgaaagacc gcaatggcat ggtgatcggc
     1921 gctggcgccg gcgttttcat tcttgaggcg gaagagattg ccgccgagcg caacgcttat
     1981 gtgctcgcgg agctcgctgg ctatggcacc acaagcgacg ccaaggaccc tgtgcggccg
     2041 gatgtgtttg gcgctgcctc gtgcatgcgc aatgcgatcg aggacgccgg cgtgacgccg
     2101 gacgagatcg attacgtcaa cgcgcacggg acaggtacga cggtcaacga tctgaccgaa
     2161 tccgcggcga tcggagtcgt ctttggcaag cggggaccac atgtgccggt gtcctcgacg
     2221 aagcccatcc atggccacgg ccttggcgtc ggtggcgcct ttgaattgat cgtcacgctc
     2281 ggcgccttgc gtgagaatgt tgcgccgcca acgatcaact ggcaggtcgc tgacgaaaaa
     2341 tgcccggtcg attgcatccc gaacgaagcg cggccgatgc cgatcaatgt cgccatgtcg
     2401 aactcgttcg ctttcggagg catcaatgcg acgctcgtcg tgcgccgtct cgctgcctga
     2461 gccgatcgag attgggggac gccatgtcgg gtgagcgggc ccttgggacg tttgttgtac
     2521 gggcggcggc tgctgaggcg gcggcatttc gccgtgaaac cggcggtacg gggcagggtg
     2581 ttcccttcac attccccgtg cgctggctgg cgcgggcaga cttccacgcg gtggctgtcg
     2641 acatgatcgg cgacggcaat tgggtgccga ttcacgagtc gcagagcttc gattatcgga
     2701 cgcccctcgc gtccgacacc gactatgaaa tggcggtgac gatgaagcgc gaaatcgaac
     2761 cgtcgcgcat cgtgctggtc gcgcagatcg gccacccgaa cgacgcgccg catctcacga
     2821 tggagatgat cctgcggatc atccccatgc cgacggagca ggccgcgtga cgcttctggc
     2881 taaatccgag caagagaatt tgcgcgc
//
LOCUS       DASWOG010000221         5999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_23486,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000221 DASWOG010000000
VERSION     DASWOG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23486"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VGB93_04550"
     CDS             complement(<1..977)
                     /locus_tag="VGB93_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145416.1"
                     /translation="MSSSFVVGNLKYLRRPFAENIKPGQRVLVLSDTEHDPRVWQVVM
                     SIITELGAEPTVALFERRPADYYDPPAAVCEAMLHSHVNVLLASTGMLHSPANFAAMK
                     AGIPAICMDGGMTLEWMQSGAVEDDMADIARRKHYVAARVFGTDAKECRVTSRYGTDF
                     IYSVEDRIFVPPLPKPDFNAYKIINFDKDENRKGGNLYYYLFPTGEYNVAPVEGSANG
                     KLVIDLTMHHLGRLITPIELTVKQGRVTSIDGGAEARILRDYLTVYGDENAYMCPAEA
                     SVGVNAKAVIRGIQREDKNIMGAMHFGLGTNIDVGGSVKSKIHMDGVVL"
     gene            complement(1384..1459)
                     /locus_tag="VGB93_04555"
     tRNA            complement(1384..1459)
                     /locus_tag="VGB93_04555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Glu,seq:ttc)
     gene            complement(1539..3362)
                     /gene="recJ"
                     /locus_tag="VGB93_04560"
     CDS             complement(1539..3362)
                     /gene="recJ"
                     /locus_tag="VGB93_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0145417.1"
                     /translation="MSQPLPFAPNRPLLGVTSSALGRPWRARLDAAGQAQALAMMQHH
                     GLSDLLARVLAGRGIAVEDAMAYLDPTLRQLLPDPSSLADMDAAAARLGAAIRAGETI
                     AIFGDYDVDGAASAALLDDFLSAAGTPHLIHIPDRIFEGYGPNVQAITALGEAGATLL
                     VCVDCGTTSHVPLAEARRLGLDVIVLDHHQAPEQLPAALVVNPNRQDDLSGLGQLCAA
                     GVVFLTLIAVNRVLRQADFWSAARAAPDLLTGLDLVALATVADVAPLTGLNRAFVTKG
                     LALMRGRERLGLAALFDVAGAGGPPSAHHLGFLIGPRINAGGRIGDAALGAKLLASRD
                     SIAARDIAETLNRLNRERQTVEAVTLEIATAEALAAAERVEASAAVLVAGDGWHPGVV
                     GLVAARLRERFRRPAFAIAFEGETGTGSGRSIPGVDLGKIVRAAVDAGLLVKGGGHAM
                     AAGITLTRGQLGAFNVFLESELERKVAAALAAEALLVDGSLSAAGASLELHLALEKAG
                     PFGAGNPEPIFVFPAHRLIDVAPVGEGHLRWRAKAADGAEIGGILFRAAQEPLGLALS
                     AARGQPLHLAGSLVCNRWNGRERMEVRLLDVVGVETGGGSA"
     gene            3500..3772
                     /locus_tag="VGB93_04565"
     CDS             3500..3772
                     /locus_tag="VGB93_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145418.1"
                     /translation="MRLDMMLGGFVGMMIGLEAVAVRDVRVVAGEVMFVLFVVLGGFA
                     MMLGGLFVMVGGGLMMLGFVQGAHFFVPRKAAGAANGPCRAKMPKR"
     gene            4239..5534
                     /gene="murA"
                     /locus_tag="VGB93_04570"
     CDS             4239..5534
                     /gene="murA"
                     /locus_tag="VGB93_04570"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0145419.1"
                     /translation="MDRIRIVGGHALEGTIAISGAKNAALPLMIASMLTRDTLTLENI
                     PRVADVGMLLQILSHHGIDYSVDGRRPGDDPHASRTLHLTARDIVDTTAPYDLVAKMR
                     ASFWVIAPLVARMGEARVSLPGGCAIGTRPVDLLLMVLEKLGAEIEIESGYVRATAKN
                     GLVGAEIHFPKVTVGGTHTALMAASLAKGHTLITNAACEPEVTDVADCLIKMGAKIKG
                     VGQSTLEVEGVGSLSGATHSVVPDRIEAGTYAMAVAMAGGDVLLERASPDHLGSALEV
                     LGQAGAAITINNQGIRVRRNGTGISPVDVETAPFPGFPTDLQAQFMALMTKAKGNSRI
                     TESIFENRFMHVQELARLGAQIRLDGDVAYVKGVKELQGAPVMATDLRASVSLVIAAL
                     AAKGETVVHRVYHLDRGFERLEQKLGRCGAVVERISGSN"
     gene            5609..>5999
                     /locus_tag="VGB93_04575"
     CDS             5609..>5999
                     /locus_tag="VGB93_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEY0145420.1"
                     /translation="MTAPLRLIALDTEDLAIMSAHLQDSLVTVGEMAYLPRSKRFAAV
                     AARFDWVAEAEGKKERCETGFHFERVLKVSRSGFSQQDPTQRLMLLSVAFDEIDSPAG
                     QVVLTFSGGAAIKLDVECLEAQVQDLGR"
BASE COUNT         1192 a         1838 c         1869 g         1100 t
ORIGIN      
        1 tccagaacca cgccgtccat atggatcttc gatttgacag atccacccac atcgatgttc
       61 gtgccaagcc cgaaatgcat ggcgcccatt atgtttttgt cttcgcgctg gataccgcgg
      121 atcaccgcct tggcattgac gccgacagag gcttccgcag gacacatgta ggcgttttca
      181 tcgccataga cggtcaggta gtcgcgaagg atacgcgctt ccgcgccccc gtcgatagaa
      241 gtcaccctgc cctgcttcac agtcagctcg atcggcgtga taagccggcc gagatgatgc
      301 atcgtgagat cgatgaccaa cttgccgtta gcgctgcctt cgaccggggc gacattatat
      361 tcgcctgtgg ggaacagata ataatagaga ttgccgcctt tgcggttttc atccttatcg
      421 aaattgatga tcttataggc gttgaaatct ggtttcggca gcggcggtac gaagatgcga
      481 tcttcgacgc tgtagatgaa gtccgttccg tagcgtgaag tgacccggca ttccttggca
      541 tcagttccga aaacgcgcgc agcgacgtag tgcttgcgcc gagcaatatc ggccatgtcg
      601 tcctcgaccg caccggattg catccattcc agcgtcatgc cgccgtccat gcaaatggcc
      661 ggaatgccgg ccttcatcgc ggcgaagttt gccgggctgt gcaacatgcc ggtcgacgct
      721 aggagcacgt tcacgtggga atgcagcatc gcctcgcaaa ctgctgcagg cggatcgtaa
      781 tagtcagccg gacggcgctc aaagagcgca actgtcggct cggcgcctag ctcggtgatg
      841 atgctcatca caacctgcca gacgcgggga tcatgctcgg tgtcggagag aacgagaaca
      901 cgctgacccg gtttaatgtt ctccgcgaac ggtctccgca aatatttgag atttccgacg
      961 acaaacgatg acgacatacc taggcgcccc ccgcaattat gatttttaag ttcaccaaac
     1021 ggcgtagtgt cgacattaaa gaggccgctc atgcgcgtca agcatcggaa agcgcccgct
     1081 tcgtgacata tgtcgacaat tttattagca aggggatcca actgtcgcgg aaatcggtca
     1141 ccgcgtttgc aattgattcg atgtatcgag gcgatttgct ggcagaaaca cacccttcga
     1201 cagggccacg gttcggccgc gttaagccga taggagcttc caagggttta ggcgtgaaaa
     1261 tctgagatcg aagggacctg atttacgaaa gtgtcctcaa gcaaaggctc ggaaagaccc
     1321 acgcggaaga ttctatcgcc aaccgtgaat aaaaatcgcg gtgttcgcag cgaacccgaa
     1381 gtatggcgcg cccaagggga atcgaacccc tgtttttgcc gtgaaagggc aacgtcctag
     1441 accgctagac gatgggcgcg tgcgggccga agcacggacg ggcgaggtat agagagcgtc
     1501 gcccgcgctg gcaagccgcg agccggcatc gagcaatatt atgcacttcc gccgcccgtc
     1561 tccacgccaa cgacgtcgag cagccgcact tccatccgct cgcggccgtt ccagcggttg
     1621 caaacgaggc tgcccgcaag atgcagtggc tgcccgcgcg cggcgctgag cgccaggcca
     1681 agcggctcct gcgccgcgcg aaagagaatg ccgccgatct ccgccccgtc tgccgccttg
     1741 gcccgccagc gtagatgccc ctccccgacc ggtgccacgt cgatcagccg atgcgccgga
     1801 aagacgaaga taggttccgg atttccggcg ccaaatgggc ctgctttttc gagcgcgaga
     1861 tgaagttcaa ggctcgcgcc cgcggcgctg agcgagccat cgaccagcag ggcctcggca
     1921 gcgagcgcgg ccgccacttt tctttcgagc tcggattcca gaaaaacatt gaaggcgccg
     1981 agttgtccgc gtgtcagcgt gatcccggcg gccatggcat ggccgccacc tttgacgaga
     2041 agtcccgcgt caaccgcggc ccggacgatc ttgccgagat cgacgccggg aatggagcgg
     2101 ccggagccgg tgcctgtctc gccctcgaag gcgatggcaa aggccgggcg gcgaaatcgc
     2161 tccctgagtc gcgccgcgac aaggccgacc acgcccggat gccagccatc gccggccacg
     2221 aggactgccg cactcgcttc gacgcgctct gcggctgcga gagcctccgc ggtcgcgatt
     2281 tccagcgtca ccgcctccac ggtctgccgc tcgcggttca gacggttcag ggtctcggcg
     2341 atgtctcgcg cggcaattga atcgcggctt gcaagcaatt tcgcgccaag cgcagcgtcg
     2401 ccgatccggc cacccgcatt gatgcggggc ccgatcagga agccgagatg atgcgccgac
     2461 ggcgggcccc ccgcgccggc aacatcgaac agcgcagcga gcccaagccg ctcgcgcccg
     2521 cgcatcaagg caagaccttt ggtgacgaag gcgcggttca ggccagtcaa aggcgcgaca
     2581 tcggcaaccg tcgcaagcgc gacaagatcg aggcccgtca gcagatccgg cgcggcccgc
     2641 gccgcactcc agaagtccgc ctgccgcaag acacgattga cggcgatcag ggtgaggaag
     2701 acgacaccgg ccgcacaaag ctggccaagg ccggagagat cgtcctgccg gtttggattg
     2761 acgacgaggg cagccggcaa ttgttccggc gcctgatgat gatcgagtac gatgacgtcg
     2821 agacccagac ggcgcgcctc ggccagcggc acatggctcg tcgtgccgca atcgacgcaa
     2881 acgagaagcg tcgcccccgc ctcgcccagg gccgtgattg cttgcacatt cgggccgtag
     2941 ccctcgaaaa tccggtccgg aatgtggatc agatgcggcg tgccggccgc ggacaggaaa
     3001 tcgtcgagaa gcgcagcaga cgccgcgcca tcgacgtcgt aatcgccgaa gatggcgatc
     3061 gtctctcccg ccctgatcgc cgcaccgagc ctcgcggccg ccgcatccat gtcggcaaga
     3121 ctggaaggat caggcaagag ctggcgcaag gtcgggtcga gataggccat ggcgtcctcg
     3181 acggcgatcc cccgccccgc gagcactcgc gccaaaagat cggagaggcc atgatgctgc
     3241 atcatggcga gggcctgcgc ctgcccggcc gcgtcgagcc gcgcacgcca gggccggcca
     3301 agggccgagc tggtgacccc cagcaggggc cgattcggtg caaaagggag aggctggctc
     3361 acgccccaat ctaacagagg cgcgcgcgca aatcagcact ttggcgtttc cttcaacagg
     3421 tcagcgtggc gccggagaaa ccaaggcgcc gccagtgtga cgacaccttg gcagatcaat
     3481 atcaggcgtg cgaatgcgaa tgcgcctcga catgatgctt ggcggcttcg tcggcatgat
     3541 gatcggcctg gaggccgtgg ccgtgcgcga tgtgcgcgtg gtggccggag aggtgatgtt
     3601 cgttctcttc gtggtgcttg gcggcttcgc gatgatgctc ggcggccttt tcgtgatggt
     3661 cggcggcggc ttgatgatgc tcggctttgt tcaaggtgct catttttttg tccccagaaa
     3721 ggcggctgga gccgcgaatg gaccctgccg ggccaagatg ccaaagcggt gacattcaca
     3781 tgaaggattg gcgaatttcc gggagagata gtggaataaa aacaaatgtt tatcgcagac
     3841 tcaccgcgga aaaattgttt ctcgaaagtc gcggaggcga gaaaaatcga accatttcaa
     3901 gctcgtgaga tcaggcttgg cgttgcgccg gggccacagg ccggcaccaa ttatgcgggg
     3961 gcgcggcgcg gccatagttt cgtccgggcc agccccttag gttgatagac ttcgtatttg
     4021 aagatgattt ggcatcgctg cgggacaaca tggtgagcga cgccgacgat aaagagaaga
     4081 cgccgcgccc tgaaaaagct tcggagcggg cgcgccttgc ggaggcgttg cgcgaaaact
     4141 tgcgccgccg aaaggtgcaa aaccgcgcac gcacaaagag cggggcaaag acagccccgg
     4201 agccggactc gccggcctag ttcgatttag ggggacagat ggatcgcatc cggatcgtag
     4261 gcggacacgc gcttgagggg acgatcgcca tctccggtgc caagaatgca gccctgcccc
     4321 tgatgatcgc gtcaatgctg acgcgggaca cgctgacgct cgaaaacatc ccgcgcgttg
     4381 ccgatgtcgg catgctcctc caaatcctca gccatcacgg catcgattac agcgtcgatg
     4441 gccgccgccc cggcgatgat ccgcatgcga gccgtactct gcaccttacc gcgcgcgata
     4501 ttgtcgacac gacggcgccc tatgatctcg tcgccaagat gcgcgcgagc ttctgggtca
     4561 ttgcgccgct tgtcgcccgg atgggcgaag cccgcgtatc gctgccgggt ggctgcgcca
     4621 tcggcacgcg gcccgtcgat ctcctgctca tggtgctcga gaagctcggc gctgagatcg
     4681 agattgaatc cggctatgtt cgcgccacgg ccaagaatgg actggttggg gcggaaatcc
     4741 atttcccgaa ggtgaccgtc ggcggcacgc acacggccct catggccgcg tcgctggcta
     4801 aaggccatac gttgatcacc aatgccgcct gcgagccgga agtcaccgac gtcgccgatt
     4861 gtctcatcaa aatgggtgcc aagatcaaag gcgtcggcca atcgacacta gaggtcgagg
     4921 gcgttggctc gctgtcgggc gcaacgcatt ccgttgtgcc ggatcgcatc gaggctggca
     4981 cctatgcaat ggcggtggcg atggccggcg gcgatgtgct cttggagcgc gcgtcgcccg
     5041 atcatctcgg ttcggcgctg gaagttctag gtcaggctgg tgccgcgatc actatcaaca
     5101 accaaggcat ccgcgtgcgc cgcaatggta cgggcatttc gcccgtcgat gtcgagacgg
     5161 cgcccttccc tggtttcccc accgatctgc aagcgcagtt catggcgctg atgaccaagg
     5221 ccaagggcaa ttcgcgcatc accgagtcga tcttcgaaaa tcgtttcatg catgtgcagg
     5281 agcttgcccg actcggcgcg caaattcggc tcgacggcga cgtcgcctat gtcaaaggcg
     5341 tcaaggaact gcagggtgcg ccggtgatgg cgactgattt gcgcgcttcc gtctcgctcg
     5401 tcatcgcggc gctggcggcg aaaggcgaaa ctgttgtgca ccgcgtctat cacctcgatc
     5461 gcggtttcga gcggctcgaa cagaaactcg gccggtgcgg cgcggtggtc gaacgtatat
     5521 cggggtcgaa ctaaatcgtt ctgcgccttg atctttccgt accgcctcct cacatgcttg
     5581 ttatcgctca tgaaggagaa cctgcgccat gacggccccg ctacgtctga tcgctctcga
     5641 taccgaagat ctcgcgatca tgtctgcgca tctgcaggac tcgctcgtca ccgtcggcga
     5701 gatggcctat ctgccgcgct ccaagcgttt cgcggcggtc gccgcccgct tcgactgggt
     5761 tgccgaggcc gaaggcaaga aggagcgttg cgagaccggc ttccactttg agcgggtttt
     5821 gaaggtttca cgcagcggct tctcccagca ggacccaacg cagcgtttga tgcttctgag
     5881 cgtcgcgttc gatgagatcg atagccctgc ggggcaagtc gtcctcacct tttctggcgg
     5941 tgctgcgatc aaactcgatg tcgaatgtct cgaagcccag gtgcaagacc ttggacgcc
//
LOCUS       DASWOG010000222         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_225306,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000222 DASWOG010000000
VERSION     DASWOG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225306"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGB93_04580"
     CDS             <1..997
                     /locus_tag="VGB93_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145421.1"
                     /translation="KQNANRAAGLVRQLLAFSRRQTLRPEVLQLGDVLSELQLMMRRL
                     VGEKVSLELRHGRDLWLVKADINQFEQVIINLVVNAPDAMPNGGKIELRTANLASVDT
                     AKLNEKSLPAADYVMIEVADTGTGIPDDVRDKIFEPFFTTKEVGKGTGLGLAMVYGIV
                     KQTGGYVFCDSTVGAGTIFRILLPRHVPTQGEDEARNKEPVKKAAADLTGHGTILLVE
                     DEEAVRAFGARALSARGYTVLEAANGAEALEVADKNSKIDLIVSDVVMPEMDGPTMFG
                     ELRKRGVRAKVIFVSGYAEDAFAKNLPEGEDFGFLAKPFTLKQLIEAVKGAAPAG"
     gene            1005..1538
                     /locus_tag="VGB93_04585"
     CDS             1005..1538
                     /locus_tag="VGB93_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145422.1"
                     /translation="MCVSRRLLFAWFIAAFAASDAQMADAKAPGRSAEPVRQIELSED
                     KIAQYLAARPTFDATLARAAAAPEPRLMRVLDETARKNGFADYADYESVAINIVWILT
                     GIDPLSKKYVGVQTVTKQEAAILLSDKALSPRDHKLRVDILHAQMLTVAPMKFVTNVA
                     LVTKYYDKLLNADAAKD"
     gene            complement(1535..1915)
                     /locus_tag="VGB93_04590"
     CDS             complement(1535..1915)
                     /locus_tag="VGB93_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145423.1"
                     /translation="MRAQISFFRNYAARGAGLALAVGLASSFSMGARAFEVSEAQREA
                     CTPDAFRLCGAYIPDSDRVATCMQANVPNLSPACRAVFQTAQPASDVVQAPRRHVRKI
                     TTSYDRRYHHYHRDDDRRGTWARD"
     gene            2158..2613
                     /locus_tag="VGB93_04595"
     CDS             2158..2613
                     /locus_tag="VGB93_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0145424.1"
                     /translation="MARAASELTAHLGYWLRFVSNHVSLAFARKLESRGVTVAEWVVL
                     RELYASAPVAPSEVAERLGLTRGAITKLADRLIAKSLPVRRASLADGRAQRLGLTAQG
                     RLIVPGLSVLADENDAEFFAHLSARDRADLKRILHDLVVRHGLKIVPTS"
     gene            2651..3061
                     /locus_tag="VGB93_04600"
     CDS             2651..3061
                     /locus_tag="VGB93_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEY0145425.1"
                     /translation="MNAEQIAIARDCHEGAETDRMTFPQSVAKLMDAGFDGYLVDLRR
                     AARTFYVPDGESLDLSTAKITQDVASRFDAESIRAAIREAQALSPGYTYRGFCAKVAA
                     AGCAGYLVSFPGRRVLYFGRTGETHVEHFPGVQS"
BASE COUNT          658 a          940 c          945 g          630 t
ORIGIN      
        1 caagcagaac gccaatcgcg ccgctggtct cgtgcggcaa ttgctggcct tctcgcggcg
       61 gcaaacgctg cggccggagg ttttgcaact cggcgatgtg ctatccgaat tgcaactgat
      121 gatgcgtcgc ctcgttggcg agaaggtcag cctggaattg cggcatggcc gcgatctgtg
      181 gctcgtcaaa gccgatatca atcagttcga gcaagtgatc atcaatctcg tcgtcaacgc
      241 ccccgacgcc atgccgaacg gtggcaagat cgaattgcgc acggccaatc tggcgtcggt
      301 ggacacagcg aagctcaacg aaaaatctct tcccgccgcc gattatgtga tgattgaagt
      361 cgctgacacc ggcaccggaa ttcccgacga cgttcgcgac aagattttcg agccgttctt
      421 cacgaccaaa gaggtcggca aggggaccgg cctcggcctc gcgatggttt acggcatcgt
      481 caagcagacg ggcggctatg tgttctgcga ctctacagtc ggcgccggca caatcttccg
      541 catcctcctg ccacgccatg ttccgacgca gggcgaagac gaggcgcgta acaaggagcc
      601 cgtcaagaaa gcggcggccg atctcaccgg tcacggcacc attctgctcg tcgaggacga
      661 ggaggcggtg cgtgccttcg gcgcccgcgc cttgtcggcg cgcggctaca cggtgctaga
      721 agccgccaat ggcgccgaag ctttggaggt cgcagacaag aattccaaga tcgatctaat
      781 tgtatcggac gtcgtcatgc cggagatgga cggaccgacc atgttcggcg aattgcgcaa
      841 gcgcggcgtg cgcgccaagg tgatcttcgt atcgggctat gccgaggatg catttgcgaa
      901 gaatttgccg gaaggcgaag atttcggctt tctcgccaag cccttcaccc tgaagcaatt
      961 gatcgaggcg gtgaagggcg cggcccccgc cggttgaggc aaagatgtgc gtttcacgcc
     1021 ggctgctttt tgcctggttc atcgccgcct tcgcggcaag cgatgcgcaa atggccgacg
     1081 ccaaagcgcc gggccgttcg gctgagcctg tccggcaaat cgaattgagc gaagataaaa
     1141 tcgctcaata ccttgctgcg aggccgactt tcgacgcgac cctcgcgaga gcggcagcgg
     1201 cgccggaacc tcggctcatg cgcgtgctcg acgagactgc gcgcaagaat ggattcgccg
     1261 attacgcgga ttacgaatct gtcgccatca acatcgtttg gattttgacc ggcatagatc
     1321 cgctcagcaa aaaatatgtc ggcgtccaga ccgtgacgaa acaggaagcc gccattctgc
     1381 tctctgacaa ggcgctctcg ccccgcgatc ataagttgcg ggttgatatt ctacatgcgc
     1441 aaatgctcac ggtcgcccca atgaaattcg tgacgaatgt ggcgctggtg accaaatact
     1501 acgacaagct tctcaatgcg gacgcggcga aggattagtc gcgtgcccaa gtaccgcgcc
     1561 tgtcatcatc ccgatgataa tgatgatatc tgcggtcata gctcgtcgta atcttgcgga
     1621 cgtgacggcg cggcgcctgg acgacgtccg aagcgggttg cgccgtctgg aagaccgccc
     1681 ggcaagctgg gctcaaattc gggacattgg cttgcatgca ggtcgcgact ctgtcgctgt
     1741 cggggatata cgccccgcag aggcggaacg catccggcgt gcaggcttcc ctttgtgcct
     1801 cgctgacttc aaatgcgcgc gcgcccatcg agaaactcga cgcaagccca acggcaagag
     1861 cgagaccggc accccgggca gcgtagttgc ggaaaaagct aatctgcgcg cgcatggaaa
     1921 ttcctcttcg atgcgatcct atggaaccgt cttacatcgg caagcccgat tttaacacgg
     1981 cgggtccatc ccgagttcct ttagatagat caattcacaa gcttcgcacc cccgctagac
     2041 gagaattgaa cacgcggggg gcgctcgcag ggcggacatg acctaaccga acgttcaaat
     2101 gattttaatt gactaggaaa ctaatttcag cgaaagcggt ggaggcaagg agactccatg
     2161 gcgcgcgctg catccgaact caccgctcac ctcggctatt ggctgcgttt cgtctccaat
     2221 catgtctcgc tcgccttcgc ccgcaaactc gaaagccggg gcgtgaccgt tgccgagtgg
     2281 gtcgtcttgc gtgaactcta tgcgtcagct ccggtcgcgc ccagcgaggt tgctgagcgg
     2341 cttggactca cccgtggcgc gatcaccaag ctcgccgaca gattgatcgc gaaatcgctt
     2401 ccggtgcggc gcgcaagtct cgccgatggg cgcgcgcaac gtctggggct gaccgcgcag
     2461 gggcggctaa tcgtgccagg cttgtcggtg ctcgccgacg aaaacgacgc tgaatttttt
     2521 gctcatctga gcgcccgcga ccgcgccgat ctgaagcgca tcctgcacga cctcgttgtg
     2581 cggcacgggc ttaaaatcgt gccgacgtct tgacgtcgtc atggcggcat cgccaaaccg
     2641 ggataaacaa atgaacgcag agcaaatcgc aatcgcgcga gactgccacg agggtgcgga
     2701 gacggaccgg atgacgtttc cgcaaagcgt cgcaaagctg atggacgcgg gcttcgacgg
     2761 ttatcttgtc gatctgcgcc gcgcggcacg aactttttat gtgcccgatg gcgaaagcct
     2821 cgatctttca actgcaaaga taactcaaga cgttgcgtca cgcttcgacg cggagagcat
     2881 aagggcggcg atccgcgagg ctcaggcctt gtcgcccggc tatacatatc gaggcttttg
     2941 cgccaaggtt gccgccgcgg gctgcgctgg ctatctcgtc tccttcccag ggcgacgcgt
     3001 gctttatttt ggtcgcacgg gcgaaacgca cgtagaacat tttcctggcg tgcaatctta
     3061 ggcgagggct caagcatgtt cgatcatatc ggcatccata caaaccagtt cgatgccttg
     3121 atctctttct acgaggccgc tttagcgccg ctcggttacg gcaagctcgc aag
//
LOCUS       DASWOG010000223         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_280134,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000223 DASWOG010000000
VERSION     DASWOG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280134"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGB93_04605"
     CDS             complement(<1..741)
                     /locus_tag="VGB93_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0145426.1"
                     /translation="MTFFESLLALMAVAILLLQVSRRLGIPYPTMLAAAGVGLALIPG
                     APKIGLDPHTALVLFIAPVIVDAAFDFPLATARRLWRPLVALAVGAVLLSAAAVAWLG
                     VAMAGLPLYAAIALGAIVAPPDAAAATAVLSNVNMPRRSVAVLKGESLLNDATALLLF
                     SAATSAQHLGGFHGEWALRLAIAAPGGVLLGILLAKLYRYVVPYVTGTLGGNLLEFVP
                     CFGVWIIADRLGLSAVLCLVAFAMTISRA"
     gene            complement(907..1170)
                     /locus_tag="VGB93_04610"
     CDS             complement(907..1170)
                     /locus_tag="VGB93_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0145427.1"
                     /translation="MRIGVVAQRAGVSTSRLRFYEASGLLQPASRDANGYRSYEFRTI
                     KIVGIIEHAQNLGFSLKEIGPFLALPHEQLTNRRRSFRCFRRS"
     gene            1248..2333
                     /locus_tag="VGB93_04615"
     CDS             1248..2333
                     /locus_tag="VGB93_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEY0145428.1"
                     /translation="MSRVLVTGGSGFIAGHVIVALLQAGHEVRATLRSLSRADEVKAN
                     VRAGGADPEHGLTFVVADLDHDAGWAEAVQGCDYVQHVASPLPFEGPENEDDLIRPAR
                     DGTLRVLKAARDAGVKRVVLTSSFAAIGYGHGNRAKPFTEADWSNLASPEVGAYPKSK
                     TIAERAAWDFIQNEGGALELAVINPVAVFGPVLGPDAGSSVQITQRMLAGRVPALPRM
                     GFGLVDVLRDVADLHLRAMIDPQAKGERFLAVAGEPMWMIDVARVLRERLGAAASAVP
                     TRQMPDWLVRVLAIFIPLLRHATPQLGILRRASNEKARLVLGWSPRPNDEIIIATGET
                     LVRLGLVKPRAGGRSPARGMTAGQFTF"
     gene            2431..4029
                     /locus_tag="VGB93_04620"
     CDS             2431..4029
                     /locus_tag="VGB93_04620"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEY0145429.1"
                     /translation="MLDRDPKTSQVWLVGGGIASLATAVFLIRDAGVAGGNIHILEEL
                     DIAGGALDGARSPAQKGYVTRGGRMQEDEAYQTLWNLLESIPSLEDPKVSVRQEIRDF
                     NTKIKSYSKARLIGGDHRIVDATIYGFNTRDRLELLRILATPEHILGARRIDDFFSEH
                     FFETNFWQMWRTMFAFQNWHSAIELKRYFLRFIQEFSRIHTLSGVRRTKYNQYDSIVR
                     PIEKWLLERGVDVRFGVRVTDVDFDQADPSQRLLTRLHVRDKSGDSTIELAPDDVAML
                     TLGSITADATYAGNDTVPDLIRDRRDGAWALWETVAKKANDFGRPNTFDANIDENKWE
                     SFTLTMHGDLLLKRLVAYSGNEPGTGGLMTFVDSGWLMSIVVPHQPHFPNMPADTYTL
                     WGYGLFIDDVGDYVKKPMAKSTGKEILTELIGQLGFDDIRDAALAATDVTSVIMPYAS
                     ALFSRRIPADRPLVIPKGSANFAFLGQFVELPEDTVFTVEYSVHCAMHAVYHLYGIDK
                     KIPPIYNGLTDPKVGLKALKAAFQ"
     gene            complement(4075..>4482)
                     /locus_tag="VGB93_04625"
     CDS             complement(4075..>4482)
                     /locus_tag="VGB93_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145430.1"
                     /translation="INLNIRGMFIATHAALKHLNNNGRIIMIGSCVGERNVTPGLAAY
                     AATKGAVKMFSQSLAREVGDRAITVNNVEPGPIDTDLNPASGDWATPQIANTALKRYG
                     TPDEVAALVAFVASPEAAYITGANLTVDGGTNA"
BASE COUNT          870 a         1339 c         1400 g          873 t
ORIGIN      
        1 cgcgcgcgag atcgtcatgg cgaaggcaac gaggcacagg accgccgaca gaccgagccg
       61 atcggcgatg atccacacgc cgaaacaggg gacgaattcg agcagattgc cgccgagcgt
      121 cccggtgaca taggggacga cgtagcgata gagtttcgcc agcaaaattc cgagcagcac
      181 accgccgggc gcggcaatcg cgagccgcag cgcccattca ccgtggaagc cgccgaggtg
      241 ttgagccgat gtcgctgcgc tgaacaggag cagggccgtt gcatcgttga ggaggctttc
      301 gcctttcagc acggcgaccg agcggcgcgg catgttgacg ttgctgagaa ccgccgtcgc
      361 ggccgccgca tcgggcgggg cgacaattgc gccgagtgcg atcgcggcat agagcggcaa
      421 tccggccatc gcgacgccaa gccacgcaac tgctgcggcg ctcagaagca cggccccgac
      481 ggccagcgcg accaacggcc gccaaagccg ccgcgccgtg gcgagcggaa agtcgaaggc
      541 cgcatcgact atcaccgggg caatgaaaag tacgagggcc gtgtgcgggt cgagcccgat
      601 cttcggcgca cccggaatca gcgccaggcc cacaccggct gcggccagca tcgtcggata
      661 gggaatgccg agccgccgcg acacttgcag cagcaggatc gcgaccgcca tgagcgccag
      721 cagactctcg aaaaacgtca aactaaattc ctccacatga gcgactgcca acagcgaagt
      781 cgccgcttgg cagaatgata gatcatccgt ttttaccgtt ttcgctttcc gccccgtact
      841 ctcgcagcaa agctcgcacc tccctccggc gcttctgcac ttcgtgcaaa tgtgtatcaa
      901 tctcgcctag cttcgcctga agcagcgaaa tgatcgtctc cggttggtca attgctcgtg
      961 cggcagcgcg aggaaggggc cgatctcttt cagcgagaag ccgagattct gcgcatgctc
     1021 gatgatgccg acgatcttta ttgtgcggaa ttcataggag cgatagccgt tggcgtcccg
     1081 gctcgcgggt tgcagcaggc cgctggcttc atagaagcgc agccgcgagg tgctcacgcc
     1141 agcgcgttgc gcgactacac cgattctcat cgtagaacct tcttgacctt caaccaaggt
     1201 tgaaggttta ggctgcctac cttgcgatgc caaggtggag cgtgaagttg agcagagttc
     1261 tggtgacggg tgggtcgggc ttcattgcgg gtcatgtgat cgtcgcgctc ctacaggcgg
     1321 gtcacgaggt gcgcgcgacc ttgcgctctc tttcgcgcgc cgacgaggtg aaggccaatg
     1381 tccgcgccgg cggtgccgat ccggagcacg gtcttacttt cgtcgtagcc gatctcgacc
     1441 acgatgcggg ctgggcggag gcggtgcaag gctgcgatta tgtccagcat gtcgcctcgc
     1501 ccctgccgtt cgagggacca gagaatgaag acgatctgat ccgccccgcg cgcgacggga
     1561 cgctacgcgt gctcaaggcc gcgcgcgatg cgggcgtgaa acgcgtcgtg ctaacctcgt
     1621 cttttgccgc gatcggctat ggccacggca atcgcgcgaa gcctttcact gaggccgact
     1681 ggtcaaattt ggcgagtccc gaagtcggcg cttatccaaa gtcgaagacg attgccgagc
     1741 gcgccgcctg ggactttatc cagaacgagg gcggggcgct ggaactcgcc gtgatcaatc
     1801 cggtcgccgt cttcgggcct gtgctcgggc cggacgcggg cagttcggtg cagatcaccc
     1861 aacggatgct tgcgggccgc gttcccgcct tgccgcgcat gggcttcggc ctcgtcgatg
     1921 tgctgcgtga tgtcgccgat ctgcatctgc gcgcaatgat cgatccccaa gcgaagggcg
     1981 agcgtttcct cgccgtcgcg ggtgagccga tgtggatgat cgatgtcgcg cgcgtattgc
     2041 gcgaacgtct cggcgcggcg gcatctgcag tgccgacgcg acagatgccg gattggctcg
     2101 tacgtgttct cgcgattttc attccgctgt tacgtcacgc aacaccacaa ctcggcatcc
     2161 tgcgtcgcgc ctcgaacgaa aaagcccgcc ttgtcctcgg ctggtcgccg cggccgaatg
     2221 acgagattat cattgccacg ggggaaacgc tcgtaaggct gggcctggtg aagccgcgcg
     2281 caggcggtcg atcgccggct agaggcatga cggcagggca attcactttt tgatgctgcg
     2341 caatgcaatc tgcgcaggac gcctatatca acgggctgcc gtgctcttcg ggtgtggggg
     2401 gcgtgcgcgt atcaaaagag ggacggttcc atgcttgatc gagatcccaa gaccagccaa
     2461 gtctggctcg tcggcggcgg catcgcttct ttggccacgg cggtattcct gatccgcgac
     2521 gcgggcgtgg cgggcgggaa catccacatc cttgaggaac tcgacattgc aggcggcgcg
     2581 ctcgacggcg cgcgctcacc ggcacagaag ggctatgtca cgcgcggcgg gcggatgcag
     2641 gaggacgaag cctatcagac gctgtggaac ctgctcgaat ctattccgtc tcttgaagac
     2701 ccgaaggtga gcgtgcggca ggaaatccgc gacttcaaca cgaagataaa atcttattcg
     2761 aaagcgcggc tgatcggcgg cgaccacagg atcgtcgatg caacaatcta tggcttcaat
     2821 acgcgagacc ggctggagtt gctgcgcatt ctcgcgacgc ccgagcatat tcttggcgcg
     2881 cggcggatcg acgatttctt ctcggagcat tttttcgaga cgaatttctg gcagatgtgg
     2941 cgtaccatgt tcgccttcca gaactggcac agcgccattg agttgaagcg ctatttcctg
     3001 cgcttcatcc aggaattctc gcgcatccat acgctctcgg gcgtccgccg gacaaagtac
     3061 aatcaatatg attcgatcgt ccggccgatc gagaaatggc tgcttgagcg gggcgtcgat
     3121 gtccgtttcg gcgtccgggt caccgacgtc gatttcgatc aggccgatcc gtcacagcgg
     3181 ctgctaacgc ggcttcatgt ccgcgataaa tccggtgatt caacgataga gctcgcgcca
     3241 gacgatgtcg ccatgctgac gctgggctca atcactgccg atgccactta cgccggcaac
     3301 gatacggtac ccgacctgat ccgcgaccgc cgcgatggcg catgggcttt gtgggagacg
     3361 gtcgcgaaaa aggcgaacga tttcggccgt cctaatacgt tcgacgccaa tatcgacgaa
     3421 aacaaatggg aatctttcac gctcaccatg catggcgatc ttctgctgaa gcggctcgtc
     3481 gcctattcgg gcaacgagcc cggcacgggc ggcctgatga cctttgtgga ttctggctgg
     3541 ctcatgtcga tcgtcgtgcc gcatcagccg cattttccga acatgcctgc cgacacttat
     3601 acgctctggg ggtatggcct cttcatcgac gatgtcggcg attacgtcaa aaagccgatg
     3661 gcgaaatcga caggcaagga aatcctgacc gaattaattg gccagcttgg cttcgacgat
     3721 attcgcgacg cagcgcttgc cgcgaccgat gtgacaagcg tgataatgcc ctatgcttcg
     3781 gccttgttca gtcggcgcat tcccgcagac cggccactgg tgatcccgaa aggcagcgcc
     3841 aatttcgcct ttctagggca attcgtcgaa ctgccggagg atacggtctt caccgtggaa
     3901 tattccgttc attgcgccat gcatgcggtg tatcatttgt acggcatcga taagaaaatc
     3961 ccgcccatct ataatggcct aacggacccg aaagtcggcc tcaaggcact caaagcggcg
     4021 tttcagtaag gcgaagccgg cgccgtagaa tattctgcgg cgccgggcat tgaactaggc
     4081 gttggtgccg ccatcaacgg tcagattggc gccggtgata taggccgcct cggggctcgc
     4141 gacaaaagcc acaagtgcgg ccacttcgtc cggggtgcca tagcgcttga gggcagtgtt
     4201 tgcgatctgc ggcgtcgccc aatcgcccga ggcggggttc aaatcggtgt cgatcgggcc
     4261 gggctcgaca ttgttgaccg tgatcgcgcg gtcaccgact tctctcgcca agctctgcga
     4321 gaacatcttc accgcgcctt tcgtggcggc ataggcagcg aggcccggcg tcacgttgcg
     4381 ttcgccgaca caggagccga tcatgatgat gcgaccgttg ttgttgagat gctttagcgc
     4441 ggcgtgggtc gcgatgaaca tgccacgaat gttgagattg at
//
LOCUS       DASWOG010000224         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_353341,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000224 DASWOG010000000
VERSION     DASWOG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353341"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="mobB"
                     /locus_tag="VGB93_04630"
     CDS             complement(<1..570)
                     /gene="mobB"
                     /locus_tag="VGB93_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY0145431.1"
                     /translation="MRIVGFAGWSGAGKTTLIVKRIPALLARGLSVSTVKHAHHNFDV
                     DKPGKDSYEHRAAGATEVLVASDRRFALMHELRGEPQPSLADLLGKLSPVDLVLVEGF
                     KRDTHMKLEVYRAANGKPLLYPEDSNIRALVSDLPPGDVPPHLTHAHIDDIEGIASLV
                     LQLAAPRPYLSRFEIRASADGGAERGDGAP"
     gene            complement(570..1337)
                     /locus_tag="VGB93_04635"
     CDS             complement(570..1337)
                     /locus_tag="VGB93_04635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0145432.1"
                     /translation="MAKGHATLVTNQFGSRADAYVESSVHAQGADLDQAAVLLDGASD
                     ARVLDLGCGGGHVAFRAASLAHDVVAYDLSTDMLAAVARAAGQRGLTNIATRQGSAET
                     LPFDDADFHFVLSRYSAHHWHGFHLALAEAHRVLRPSGRALFMDAISPGTALLDTCLQ
                     TVELLRDPSHVRDYSAAEWVNALAGAGFAVKGVTTRRLRLDFGTWIARMATPEVQTAA
                     IHALQTQMSEDVVKHFEIEADGSFTIDTAAFEVSRDA"
     gene            1387..1617
                     /locus_tag="VGB93_04640"
     CDS             1387..1617
                     /locus_tag="VGB93_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY0145433.1"
                     /translation="MAERVKELDLRGLKCPLPVLRTRKALARLGPGEGLVVFCTDPPS
                     RIDIPHLIHETGDVLEQSRVDGGVFIFHILRR"
     gene            complement(1614..2243)
                     /gene="mobA"
                     /locus_tag="VGB93_04645"
     CDS             complement(1614..2243)
                     /gene="mobA"
                     /locus_tag="VGB93_04645"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY0145434.1"
                     /translation="MAEPLPSTGAIILAGGRATRMAGADKASQMLGATPLIAHVLAAL
                     KPQCGALVINANGDEARFAALGMPVVSDDVPGFAGPLAGILAGLDYLAGHYPDLTYAA
                     SAATDTPFLPDDLVAKLHAARIAAKAEIAVARSDNIVHPTFALWPIDLRTDLREALVD
                     EDLRRVTTFFSRYRCAYTNWSAEPFDPFFNVNTLDDLRIAERILADRKT"
     gene            complement(2275..2619)
                     /locus_tag="VGB93_04650"
     CDS             complement(2275..2619)
                     /locus_tag="VGB93_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145435.1"
                     /translation="MLLGRSFRVEGFDCFADLEGSYRVEGDDEPDEWHADATLGVRPR
                     RDILLLVQSFATIAAQATQASPRYTWGKLQESAVYDISARWSVEGDFFEPVAGSEAGR
                     ELGPTAALWYRF"
     gene            complement(2582..>3013)
                     /locus_tag="VGB93_04655"
     CDS             complement(2582..>3013)
                     /locus_tag="VGB93_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145436.1"
                     /translation="RRPCFLLGIALASNSVAQSGASAGAWLQPQGEGLAIAATDFSDS
                     TRYFDSHGKLIPIPAYQKFSLGTYIEYGLSDEFTLVAQPFFDSDKQSTSPSPTRLTTG
                     GAQITAHVPFVTRTPLVNFDEDTVPAGDSFAARSVVPGRRL"
BASE COUNT          569 a          905 c          979 g          560 t
ORIGIN      
        1 gggcgcgccg tcgccgcgct cggcgccgcc atccgcgctc gcgcgaatct cgaagcggct
       61 taggtacggt cgaggcgctg ccaactgcaa aacaagcgac gcgatgcctt cgatatcgtc
      121 gatgtgcgcg tgcgtcagat gcggcggcac gtcgccgggc ggaagatcgc tcaccagcgc
      181 cctgatgttg gaatcctcgg ggtagagcag tggcttgcca ttggcggcgc gatacacttc
      241 gagcttcatg tgtgtatcgc gtttgaagcc ttcgacgaga acgagatcga ccggcgaaag
      301 tttaccaagc aggtctgcaa gtgatggctg cggctcgcca cgcaattcgt gcatcagcgc
      361 gaaccgtcga tccgaggcga cgaggacttc cgttgcgccc gccgcgcgat gttcgtagga
      421 atctttgccc ggcttatcga catcgaaatt atgatgcgca tgcttgacag tagagacgct
      481 gagaccgcgc gcgagaagcg ctggaatgcg cttgacgatc agggtcgtct tgccggcacc
      541 gctccagcct gcaaacccga caatgcgcat taggcgtccc gcgacacttc gaaggcggcg
      601 gtgtcgatgg tgaagctgcc gtcggcctcg atctcgaaat gtttgacaac atcctcggac
      661 atctgcgttt gtaacgcgtg gatcgccgct gtctgcactt ccggcgtcgc catgcgtgcg
      721 atccaggtgc caaaatcgag acgcaatcgc cgcgtcgtga cgcccttcac cgcgaagccg
      781 gcgccggcaa gggcgttcac ccattcggcc gccgaataat cgcgtacatg cgagggatcg
      841 cgaagcagtt cgaccgtctg gagacaggtg tcgagcagtg cggtgcccgg cgagatcgcg
      901 tccatgaaaa gtgcccggcc gcttggccgc aacacgcgat gcgcttcggc aagcgcgagg
      961 tggaaaccgt gccaatgatg cgcgctatag cggctcaaaa cgaaatggaa gtccgcgtcg
     1021 tcgaagggta gggtttccgc gctgccttgc cgcgtcgcga tgttcgtgag gccgcgttgc
     1081 cccgccgcgc gcgcaacggc cgcgagcatg tcggtcgaga gatcgtacgc caccacgtcg
     1141 tgcgcgagcg acgcggcgcg aaacgcaaca tgaccgccgc cacagccgag atcgagcacg
     1201 cgcgcgtcgc tggcgccgtc cagaaggacg gctgcctgat cgagatcggc gccttgcgcg
     1261 tggacgctgc tttcgacata agcgtcagcg cggctgccga attgattggt caccagcgtg
     1321 gcatggcctt tggccatctt attctccgtc tcgcttggat agcgtcaatc tgtctatatg
     1381 catcgcatgg ccgagcgggt caaagaactg gatttgcgag ggctgaaatg cccgttgccg
     1441 gtgttgcgga cacggaaggc gctggcgcgg cttggccccg gcgaaggcct tgtcgtcttc
     1501 tgtactgatc cgccttcccg catcgacatc ccgcatctca ttcatgaaac gggcgatgtt
     1561 ctggaacaaa gccgcgtgga tggcggcgtg tttatctttc atatcttgcg gcgttaggtc
     1621 ttgcggtctg ccaatattcg ttcggcaatg cgtaagtcgt ccagcgtatt gacgttgaag
     1681 aacgggtcga aaggttccgc gctccaattc gtgtaggcgc aacgatagcg gctgaagaac
     1741 gtcgtgaccc ggcgtaaatc ttcatcgaca agcgcttcgc gcagatcggt gcgcagatcg
     1801 atcggccaaa gcgcgaaggt cggatggacg atattgtcgg aacgggccac cgcgatctcg
     1861 gctttcgctg caatccgtgc cgcgtgaagt ttcgcgacaa gatcgtcagg tagaaacggc
     1921 gtatcggtcg cggcactagc tgcgtaagta agatcgggat aatgccccgc caaataatcg
     1981 agcccggcca gaatgccagc cagaggtccg gcaaaccctg gaacgtcgtc cgacacgacg
     2041 ggcatgccca aagccgcgaa acgcgcctca tcgccattgg cgttgatgac caacgcgccg
     2101 cattgcggtt tgagcgccgc aaggacgtgc gcgatgagcg gcgtcgcgcc gagcatttgc
     2161 gatgccttgt cggcgccggc catgcgcgtg gcccggcctc cggcgaggat gatagcgcca
     2221 gttgagggga gcggttcagc catggcgcgg atttacgcgg ctatggccca ccggtcaaaa
     2281 ccggtaccag agcgcggcgg tcggcccaag ctcgcggcca gcttcgctcc cggcgacggg
     2341 ctcgaaaaag tcgccctcaa cggaccagcg cgccgagatg tcgtaaacgg cgctttcctg
     2401 cagcttgccc caggtatagc gcggtgatgc ctgcgtcgcc tgtgcggcta tcgtcgcgaa
     2461 gctctgtacg agcagcagga tatcgcgtcg cggccgcacg ccgagggttg catcagcgtg
     2521 ccattcgtcg ggctcgtcgt cgccctcgac gcggtagctg ccctcaagat cggcgaagca
     2581 atcaaagcct tcgacccgga acgaccgacc gagcagcaaa cgaatcgccg gcaggaacgg
     2641 tgtcttcgtc gaagttgacc agcggcgtgc gggtcacgaa tggcacgtgc gccgtgatct
     2701 gtgccccgcc ggtcgtcagc cgcgtcggcg atgggcttgt gctctgtttg tcggaatcga
     2761 agaacggttg cgcgacgagc gtgaattcgt cgctcaggcc atattcgata tatgttccga
     2821 gcgagaactt ctgataggcg gggatcggga tcagcttgcc gtggctgtcg aaatagcggg
     2881 tcgaatccga aaaatcggta gcggcgatgg ccaggccctc gccttgtggt tgtagccagg
     2941 cgccagcgct ggcgccgctt tgcgccaccg aattgctggc gagcgcgatc ccgagcagaa
     3001 aacagggacg ccg
//
LOCUS       DASWOG010000225         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_317140,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000225 DASWOG010000000
VERSION     DASWOG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317140"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VGB93_04660"
     CDS             complement(<1..824)
                     /locus_tag="VGB93_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY0145437.1"
                     /translation="MKAPRKTVPRKAKEPELDLPFPKDDRRLIVVRGAREHNLKNVDL
                     VIPRDKLVVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMTQKPDVDQID
                     GLSPAISIEQKTTSRNPRSTVGTVTEIYDYMRLLWARVGIPYSPATGLPIESQTVSQM
                     VDRVLALPERTRLYLLAPVVRGRKGEYRKEIAEYMKRGFQRLKIDGQFYEIAEAPALD
                     KKLKHDIEVVIDRIAVRPDLAARLAESFETALELADGIAFIEYADEKDENGKAKQIV"
     gene            1032..>1805
                     /locus_tag="VGB93_04665"
     CDS             1032..>1805
                     /locus_tag="VGB93_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY0145438.1"
                     /translation="MDATVPAAKSRTPIYKLLYAQVLFGIALGAIFGWLAPHVATADW
                     VKALGDGFVKLIKIVIAPLIFLTVTAGIAHISDARKVGRVAIKAIVYFEIVSTFALAI
                     GLLVGNLVRPGAGFAARADAAAVAAYAKAAGAEHPVQYLLNIIPDTAIGAFSRGDVLQ
                     VLLFSILFGFALMALGQRGKLLTDVLDQATLAVFGIISIVMRAAPVGAFGAMAYTVGK
                     YGTQVLGALAGEIATFYLTSALFVIVVLGTIARLAGFNIF"
BASE COUNT          311 a          551 c          545 g          398 t
ORIGIN      
        1 acgatctgct tcgccttgcc gttttcatcc ttctcgtcgg catattcgat gaaagcgata
       61 ccgtcggcca gttccaacgc cgtttcgaaa ctttccgcaa gccgcgccgc gaggtctggc
      121 cgtaccgcga tgcggtcgat caccacttcg atgtcgtgct tgagtttctt gtcgagcgcg
      181 ggcgcttcgg caatctcgta aaactgcccg tctattttca gccgctggaa accgcgcttc
      241 atatattcgg cgatttcctt gcggtattcg cccttacggc cgcgcacgac cggcgccagc
      301 agataaagcc gcgtgcgctc cggcagcgcc agcacgcggt cgaccatctg cgagactgtc
      361 tggctctcga tcggcaggcc agtggccggc gaatagggaa taccgacgcg cgcccataga
      421 agccgcatgt aatcgtaaat ctcggtgacg gtgccgaccg tcgaacgcgg attgcgcgac
      481 gtggttttct gctcgatcga aatcgccggc gaaagcccat cgatctgatc gacgtccggc
      541 ttctgcgtca tctccagaaa ttgccgggca taggccgaaa gtgattcgac gtagcggcgc
      601 tgaccctcgg catagatcgt gtcgaaggcg agcgacgact tgcccgagcc ggacagaccg
      661 gtgaacacga ccagcttgtc gcgcgggatg acgaggtcga cgttcttcag attgtgctcg
      721 cgcgcgccgc gcacgacgat gagccgccgg tcgtccttgg ggaacggcag atcgagttcg
      781 ggctctttgg ctttgcgggg gacggttttg cgcggtgctt tcatggactt tcgtagaata
      841 tcttggctct ccggagagag gaaatatcgg gctggacgtc gcggcgagtt gcgcgcggct
      901 taaggttcgt cgaagatcgg aacgctttaa gataggggct cgttaggtca aggccaagag
      961 cgcctcttcg gcgcgggcga ccgagcctga gaagcatgcc caagaagaat gaaaacgcca
     1021 gcaggaacgc catggatgcc accgtccccg ccgccaaaag ccgaaccccg atctataagc
     1081 tcctctatgc gcaggttctg ttcggcatcg ctctcggtgc gatctttggc tggctcgccc
     1141 ctcatgtcgc caccgccgat tgggtgaagg cgctgggtga cggcttcgta aaactcatca
     1201 aaatcgtcat tgcgccgctg attttcctga cggtgacggc gggcatcgcc catatttccg
     1261 acgcccgcaa ggttggccgc gtcgcgatca aggcaatcgt ctatttcgaa atcgtctcga
     1321 ccttcgccct ggcgattggc ctcctcgtcg gcaatctggt ccgccccggc gcgggctttg
     1381 ccgcgcgtgc cgacgccgcc gctgttgcgg cctatgccaa ggccgctggc gcggaacatc
     1441 cggtccaata tctgctcaac atcatccccg atactgccat cggtgctttt tcgcgcggcg
     1501 acgttttgca ggtgcttctt ttttcgatcc tgtttggctt tgccctgatg gcgcttggcc
     1561 agcgcggcaa gcttctgacc gacgtgctcg accaggcaac actcgcggtt ttcggaatca
     1621 tcagcatcgt gatgcgggca gcgcccgtgg gcgccttcgg ggccatggcc tatacggtcg
     1681 gcaaatacgg gacgcaggtg ctgggcgctc tcgccggcga gatcgcgacg ttttacctga
     1741 cgtcggcact ttttgtcatt gtcgtgctcg gcacgatcgc ccggttggca ggcttcaata
     1801 ttttc
//
LOCUS       DASWOG010000226         5055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_262125,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000226 DASWOG010000000
VERSION     DASWOG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262125"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB93_04670"
     CDS             <1..300
                     /locus_tag="VGB93_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0145439.1"
                     /translation="LRGAVLPIVDLAARLGYPATDPTVRHVIMVVQVERQVVGLLVDA
                     VLDILTVADTDIQPTPDVASEMAKLFTRGVLAIDGRMISWIALEHVLPPVASEAA"
     gene            472..2286
                     /locus_tag="VGB93_04675"
     CDS             472..2286
                     /locus_tag="VGB93_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY0145440.1"
                     /translation="MTLAASAPAERSGEPDPVGIIARWRGLVRRSEPALIILAIVTGL
                     VAAALVALTSDAVQLLHQICFGVAPDVRLSGVSRLHAPYLAIVPAIGGAILSLTGIFI
                     KRWRPRRPVDPIEANALHGGRMSLIDSSLIALQTVVSSGFGASVGLEAGYTQIGSGFG
                     SLFGTTFRMRRSDMRTLVGCGAAGAIAAAFQAPLTGAFYGFELIIGTYTSFGLVPVIA
                     AAISAVLASRALGINANFVGHFVPAKSLTPFDLIAFLILALACAAVGIGVMRGVTFVE
                     SLFKRSHVPAIAQPIVGGLLVGTLALVSPHVLASGHGALASLFQTNGPALALLITIVT
                     LKSIASAISIGSGFRGGLFFASLYLGGLMGKITCALVLLLVPAVAPDVSTYIIVGMAA
                     LAVAIVGAPLTMSFLALETTGDFSLALVVLTVSALVSVVVRRTFGYSFATWRLHLRGE
                     SIRSAQDVGWMRALTVRELMRTDLHITPASQSLKDFKKQFALGSTNWVAGVDAGGRYA
                     GLINVADAHLADVEIEAGPTRLPQLLREPETVLRPELNIKEAADLFDRTQSEALAVID
                     GNGKPIGLLTEAHVLRRYTEELERARRELVGEQWLGES"
     gene            complement(2283..3218)
                     /locus_tag="VGB93_04680"
     CDS             complement(2283..3218)
                     /locus_tag="VGB93_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY0145441.1"
                     /translation="MLSTLSVVLPIFAVIFAGWLARRRELLGPAATSELNRYVVYLAL
                     PALLFDVIAHAGGREIWRPGFIAAFGLSALLCFALTVAIRLRQGSPLADAAVDGLNGA
                     YANTGYMGFPLALAAFGAGSQAAVLIATILTVCFVFALAIVLIEIGLQKEPNRLKLVL
                     RVAGTLARNPLIFSPALAAIIRLAGFGVPHPVEAFVKLLGESAAPCALVGLGLFLAEK
                     RAANESRSGVTAALTAVKLLLQPLLTWVLAAFVFHLAAFETHAAVLIAALPTGTGSFM
                     LAEFYQREATTTSRVILISTLLSVLTLSLYLAAVR"
     gene            3421..4944
                     /gene="acpA"
                     /locus_tag="VGB93_04685"
     CDS             3421..4944
                     /gene="acpA"
                     /locus_tag="VGB93_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY0145442.1"
                     /translation="MRAAALGCAALGFATSVDRAAADDLAKINTIVVLYAENRSFDAL
                     YGEFPGANGLTRLAPAQFQQHDRDGKLLPTLPAVWGGLTSKHVTPAIPQDKTAGLPNA
                     PFAIDDPEGFNLPLGVKTRDLVHRFYQNQMQIDGGKNDLFAAYSDAGGLVMGHYDGSH
                     LPLWQIAKKYVLADNFFMGGFGGSFLNHFELICACAPVYPNAAQSPAKTQISVVDADG
                     VALKLAPDSSPSALNGPPKFVNDGALTPDFYAINTMQPAYQPSGNPAPAGADPALSDP
                     ASPTTLPPQTETTIGDLLDDKGIDWAWYAGGWGAALAAKDPKTIKALIFHHQPFNYFK
                     FLAPGTKARTAHLRDAGLSGELLVKDIDAGHLPPVTFYKPSGNLDEHPGYADVLSGDA
                     HLADLVAHLEKSPQWPHMVVVITYDENGGFWDHVAPPKGDRFGPGTRVPTLIISPYAR
                     RGLVDHTLYDTTSILRLITKRFGLPELAGLEVRDAAMAKVGEPKLGDLTPALDLTGK"
BASE COUNT          912 a         1561 c         1598 g          984 t
ORIGIN      
        1 ctgcgcggcg cggtgctacc gatcgtcgat ctcgcggccc ggctcggcta tcccgccacc
       61 gatccgacgg tgcgccacgt catcatggtc gtgcaggtcg agcgtcaggt ggtcggcctg
      121 ctcgtcgatg ccgtgctcga tatcctgacg gttgccgaca ccgatattca gccgaccccc
      181 gatgtcgcct ccgaaatggc caaattattc acccgcggcg tgctcgcgat cgacgggcgc
      241 atgatcagct ggatcgcgct ggagcatgtt ttgccgccgg tggcgagcga agcggcgtaa
      301 ggcgggatag tcttaaaaag ccacgcggct tcgacggctg ttgtttatcg cctttggaac
      361 gattgtcttt gcagcgcgtc tgcgtcatat ggccgatcga ttccaatcat gttggcgcgg
      421 gatttagcgg gactgcaccg cttttcgcat cccgctccag cgaggacggc gatgaccctt
      481 gccgcttcgg caccagccga gagatctggc gaacccgacc ccgtcggtat catcgcgcgc
      541 tggcgcggtc tggtccggcg cagcgaaccg gcgctgatca ttctggcgat tgtcacgggt
      601 cttgtggcag cggcgctggt ggcgctgacc tccgacgcag tccagttgct ccaccaaatt
      661 tgtttcggcg ttgcgcccga tgtgcgcctt agcggcgtgt cgcgcttgca cgcgccctat
      721 ctggctatcg tgcccgcgat cggcggcgcg atccttagcc tcacgggtat cttcatcaaa
      781 cgctggcggc cacgccggcc ggtcgatccc atcgaggcca acgcgctgca tggcgggcgc
      841 atgtcgctca tcgacagcag cctcatcgct ctgcaaacgg tggtgtctag cggcttcggc
      901 gcctcggtcg ggcttgaggc cggttatacg cagattggtt cgggtttcgg atcgcttttc
      961 ggcacgacat tccgaatgcg ccgtagcgac atgcgcacgc tcgtgggctg tggcgcggca
     1021 ggcgccatcg ccgcggcgtt tcaggcgccg ctgacgggcg ccttctacgg ttttgagctg
     1081 attatcggca cttacacttc gttcggtctt gtaccggtca ttgcggccgc gatcagcgcg
     1141 gtgctcgcgt cgcgtgcgct cggcatcaat gcaaatttcg ttggtcattt cgtgccggcg
     1201 aaatcgctga cgccgttcga tctcatcgct tttctgattc tcgcgctggc ttgcgccgcg
     1261 gtcggtatcg gcgttatgcg cggtgtgacc ttcgttgaga gcctgttcaa acgcagccac
     1321 gttcccgcca tcgctcagcc gatcgtcggc ggtctccttg tcggcactct ggctctggta
     1381 tcgccgcatg tcctggcctc ggggcatggc gcgctcgcct cgctttttca gaccaacggg
     1441 ccggccctcg cgctgctgat tacgatcgtg actttgaaat caattgcctc cgccatttcg
     1501 atcggctcgg gctttcgcgg cggcctgttc ttcgcctcgc tctatctcgg cggcctgatg
     1561 ggcaagatca cgtgcgcgct cgtgctcctg ctcgtccctg ccgtggcgcc cgatgtttcc
     1621 acctatatca tcgtcggcat ggcggcgctg gccgttgcaa tcgtcggcgc gccgctgacc
     1681 atgagcttcc ttgcccttga gacgacggga gacttttcac tggcgctggt ggtgctgaca
     1741 gtttccgcgc ttgtctccgt cgtcgtgcgc aggacgttcg gctattcctt cgcaacatgg
     1801 cggctgcatt tgcgcggcga atcgatccgc agtgcgcagg atgtcgggtg gatgcgcgcg
     1861 ctgaccgtgc gcgagctgat gcggaccgat ctgcacatca cgcccgcaag ccaaagtctg
     1921 aaggacttca aaaaacaatt cgctctcggt tcgacgaact gggtcgccgg ggttgacgcg
     1981 ggcggccgct atgccggtct catcaatgtc gccgacgcgc atctcgcaga tgtcgagatc
     2041 gaagccggac caacgcggct gcctcagctt ttgcgcgaac cggaaaccgt tttgcggccg
     2101 gaattgaata tcaaggaagc ggccgatctt ttcgatcgca cccaaagcga ggcgctcgcc
     2161 gtcatcgacg gcaatggcaa accgatcggg cttttgaccg aggcccacgt tctgcgccgt
     2221 tataccgaag aactggagcg ggcgcgacgc gaacttgtcg gcgagcaatg gcttggcgaa
     2281 agctagcgca ccgcggcgag atagagcgac aaggtcagga cagagagcag ggtggagatg
     2341 agaatgacgc gcgaggtcgt cgtggcttcg cgctggtaaa actccgccag catgaatgag
     2401 cctgtgccgg tcggcagcgc cgcgatcaac accgcggcat gcgtctcgaa ggccgcgagg
     2461 tgaaagacga aagccgccag cacccaggtg agcaaaggct gcagcagaag tttgacggcg
     2521 gtgagtgcgg ccgtcacacc ggagcggctt tcattcgcag cgcgcttttc ggcgagaaac
     2581 aggccgagcc caacgagcgc acagggcgct gccgattcgc ccagcaattt cacaaaggct
     2641 tcgactggat gcggcacgcc gaagccggca agtcggatga ttgcggcgag agcgggcgaa
     2701 aagatcagcg gattgcgcgc cagcgtgccg gcaacgcgga gcaccagctt caggcggttt
     2761 ggctcttttt gcaggccgat ctcaatcagc acgatcgcca gcgcaaacac gaaacaaacg
     2821 gtcaggatcg tcgcaatgag aacggctgcc tgcgacccgg cgccgaacgc ggccaaggca
     2881 agtggaaaac ccatgtaacc ggtattggcg taggcgccgt tgagcccatc gacggcggca
     2941 tcggcgagcg ggctcccctg gcgaagccgg atggcgaccg tcagtgcgaa gcagagcaaa
     3001 gccgaaagcc cgaaggcggc aatgaatccc ggccgccaga tctcgcggcc gccggcatgt
     3061 gcgatgacgt cgaagagcag cgccggaagc gcgagataga cgacatagcg gttgagttcg
     3121 ctcgtggccg cggggccgag gagttcgcgg cgtcgcgcca gccaaccggc aaagatcacc
     3181 gcaaaaatcg gcaggacgac cgaaagggtc gagagcatgt ggggccgcgt tgaatgttga
     3241 aacggcgcct ccgcactctt tgcagccgcc agatcctctc caatcagagg cgggcggaaa
     3301 gatcaaatgg cggtgtggga agatggtgcg gcctgagagt caggggtcgc gtcaaacctt
     3361 tgtcacaaaa ggtctatgtc taatccgctc atcggtttcg ggggttcagg tatgattgga
     3421 atgagagccg ctgcgctggg ttgcgccgcg ctgggttttg cgacgtccgt ggacagggcc
     3481 gcggcagacg atctcgccaa gatcaatacg atcgtcgtgc tctatgccga gaatcgcagc
     3541 ttcgatgcgc tctatggcga atttcccggc gccaacggcc tcacccggct cgcgcccgca
     3601 caattccagc aacacgatcg cgacgggaaa ttactgccga cgttgccggc ggtttggggc
     3661 ggtttgacta gcaagcatgt gacgccggca atcccgcagg acaagacggc aggtctgcca
     3721 aacgcaccgt tcgccatcga cgatcccgag ggtttcaatc tgccgctcgg cgtgaagacc
     3781 cgtgatctcg tgcatcgctt ttatcaaaat cagatgcaga tcgacggcgg caagaacgac
     3841 cttttcgccg cctatagcga cgcgggcggc ctcgtcatgg gccattatga tggatcgcat
     3901 ctgccacttt ggcagatcgc caaaaaatac gtattggccg acaatttttt catgggcggc
     3961 ttcggcggct cgttccttaa tcatttcgaa ctgatctgcg cttgcgcgcc ggtttatccg
     4021 aatgccgcgc aaagcccggc gaagactcag atttcggtcg tggacgcgga tggcgttgcg
     4081 ctgaaactgg cgccggattc ctcaccctcc gcgctcaacg ggccgccgaa attcgtcaac
     4141 gatggcgcgc tgacgccgga cttttatgcg atcaatacaa tgcagccggc ctatcagccg
     4201 agcggcaatc cggcgccggc gggcgccgat ccagcgcttt ccgatcccgc atcgccaacg
     4261 accctgccgc cgcagacgga aacgacgatc ggcgatcttc tcgatgacaa ggggatcgac
     4321 tgggcctggt atgccggcgg ctggggtgcg gcgctcgccg ccaaggatcc gaagaccata
     4381 aaagcgctga tcttccatca tcagccgttc aattatttca aattcctcgc gccgggcacc
     4441 aaggcgcgta cggcgcatct gcgcgacgcg ggcctgagcg gcgaacttct ggtgaaggac
     4501 atcgacgccg gccatctgcc gccagtgacg ttctacaagc cctcgggcaa tctcgacgaa
     4561 catccaggct atgcggacgt gctctccggc gatgcacatc tcgccgatct cgtcgcgcat
     4621 ctggagaaaa gcccgcaatg gccgcacatg gtcgtggtca tcacctatga tgagaacggc
     4681 ggcttctggg atcatgtcgc gccacccaag ggcgaccgct tcggcccggg tacgcgggtg
     4741 ccgacgctga tcatttcgcc atatgcccgg cgcggcctcg tcgatcacac gctttacgac
     4801 acgacctcca tcctacgttt gatcacaaaa cgcttcggcc tgccggaact cgcagggctg
     4861 gaggtccgcg acgcggcgat ggcgaaggtc ggggagccga aactcggcga tctcacgccg
     4921 gcgctggatc tgacggggaa gtgagacgcc ggctcaggcc ggctcggcga gattggcgag
     4981 cttgatcgtc gcgccctggt tttgtgcgac cctgatgttc tcgaagaact gccgcgcgct
     5041 ttccttccag gtgaa
//
LOCUS       DASWOG010000227         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_133783,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000227 DASWOG010000000
VERSION     DASWOG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133783"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGB93_04690"
     CDS             <1..236
                     /locus_tag="VGB93_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0145443.1"
                     /translation="CISQAPTTYLELTAGLNITPTLPSSIPFIKSVIFRPEVRYDASL
                     NSTTPFDAPIAAPGVLGTKSDQVTIGGDVIVKF"
     gene            390..1344
                     /locus_tag="VGB93_04695"
                     /pseudo
     CDS             390..1344
                     /locus_tag="VGB93_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1316..>1680)
                     /locus_tag="VGB93_04700"
     CDS             complement(1316..>1680)
                     /locus_tag="VGB93_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0145444.1"
                     /translation="ARRLLIADEAMLARIDAIRSESPHFSDVISLVQRAARLSLVTQS
                     PLVIPPLLLLGEPGIGKSFFARSLAEAIGTHCERIAMDMLSDHGALTGLSLSWKAARP
                     GRIALELRAFKLVHIRQH"
BASE COUNT          345 a          537 c          473 g          325 t
ORIGIN      
        1 attgtatttc tcaggccccg accacctatc tcgaattgac cgccggcctt aacatcactc
       61 cgacgctgcc gtcgtcgatc cccttcatca agtcggtgat tttccggccg gaagttcgct
      121 acgacgcgtc gctcaatagt actacaccgt tcgacgcccc tattgcggct cctggcgtac
      181 tcggaacgaa atcggaccaa gtcacgatcg gtggcgacgt catcgtgaag ttctgatctc
      241 tctcaaagaa acaatcctaa acgccggcct cagggccggc gttttttttg cgtttttgcc
      301 gcaacctaga gcgtcgggca ttcagattga atcgagggat tccctttgag ggaggctcgt
      361 gattcacttc cgttcggagg tgatttgcga tgccgaaggc ctattcgctc gatttgagtg
      421 aacgcgttgt tcagtttgtc gaagggggtc agtcacggca tgtggcggcg gctcacttcg
      481 aagtctctgt ctcctttgtg gtgaagctga tgacggccta cgccaggagc ggcagcttgc
      541 agcccaagcc cagcggaggg cggcgccatg ccaagctcga accgcatcgc gcctttctgc
      601 ttgcccgggt cgccgagaag gacgatatct cgatgccgga actgacgggc gaactcgccg
      661 cggcgacggg gacgcgggtc gatccatctt cgatctcgcg ctggttcatt cgcaacggct
      721 acagcgtcaa aaaaaaccct gctggccagc gagcaagatc gtcctgacgt caaacaggcg
      781 cgcgacgaat ggatgaacac gcgccaacct cggatgcggc ttgagccgca gcgtctcgtc
      841 ttcatcgacg agaccggcac caccacgaag atgacgcgcc cacgcggccg ttgcctcaag
      901 ggacgccggc tgcgatccaa ggcgccgttc ggccattgga aaacccagac cttcgtcgcc
      961 ggcctgcgct gttatggcct caccgcgccg ttcgtcatcg acgccccgat gaaccgacgc
     1021 atcttcgaga cctatgtcga gaaagaactc gcgccgaccc tcgaaaaggg cgatgtcgtc
     1081 atcttggata atctgtcggc ccacaaaagc gcagccgccg agagagccat ccgtgcccgc
     1141 ggtgcctgga tcctcttcct gccgccctac agccccgatc tcaatccgat cgagatggcc
     1201 ttcgccaaac tcaaagccca cctacgcgct cgcgccatca ggaccatcga cggtctttgg
     1261 cgggctatcg gccaaatctg cgacctcttc cagcccgagg aatgcagaaa ctacttcaat
     1321 gctgccggat atggaccaac ttgaatgcac gaagctctag cgcaatgcgc cccggacggg
     1381 ctgccttcca cgagagcgaa agtccggtca aggccccgtg gtcgctcaac atatccatgg
     1441 cgattcgctc gcaatgcgtg ccaatcgcct ccgccaaaga tcgtgcaaag aagctcttgc
     1501 cgatacccgg ctctccgaga agcagaagag gcgggatcac gagcggggac tgcgtgacca
     1561 acgacaatct cgccgcccgc tgaaccagac tgatcacatc tgagaagtgc ggcgattcgg
     1621 accggatcgc gtcgatcctc gcgagcatgg cttcatcggc gatcaggagg cgtcgcgcgg
//
LOCUS       DASWOG010000228         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_317176,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000228 DASWOG010000000
VERSION     DASWOG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317176"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VGB93_04705"
     CDS             <1..828
                     /locus_tag="VGB93_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0145445.1"
                     /translation="GLEAGTALVEHPRVPSVSLVGSVQSGVAVAKGAAERLKHVGLEL
                     GGKNALVVYPDADLAKAADGAIKGMNFLWCGQSCGSTSRLFLHESIYDQVLADVLAGI
                     GRHKPGLPTDPTTTMGALISRHQQEKVLRYIEIAKGEGARLVCGGKVPSTPELAGGFY
                     VEPTVFVDVTPDMRIAREEVFGPVLSVLRWSDETALFEAINSVEYGLTGSVWTKDLAT
                     AHRAAARIESGYIWVNGAGPHYLSVPFGGYKMSGLGREEYIDELFHFTETKNIHITL"
     gene            complement(867..1567)
                     /locus_tag="VGB93_04710"
                     /pseudo
     CDS             complement(867..1567)
                     /locus_tag="VGB93_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581353.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          333 a          486 c          445 g          303 t
ORIGIN      
        1 ggtctggaag ccggcaccgc tctcgtcgaa catccgcgcg tcccttcggt aagtctggtc
       61 ggcagcgtgc aaagtggcgt cgccgtcgca aaaggcgcgg cggaacggct gaagcatgtc
      121 ggtctcgaac tcggcggcaa gaacgcactt gtcgtctatc ccgacgccga tctcgccaag
      181 gcagccgacg gcgcgatcaa aggtatgaac ttcctctggt gcggccaatc ttgcggttcg
      241 acctcgcggc tgtttctgca cgagtcgatc tatgaccagg tcctggccga tgtccttgcc
      301 ggaatcggcc gacataagcc cggcctgccg accgatccaa cgacgacgat gggcgcgctt
      361 atttcccgcc accagcagga aaaagtgctg cgctatatcg agatcgccaa aggcgaaggc
      421 gcacggctcg tctgcggcgg caaggtgccg tcgacaccgg aactcgccgg cggcttctac
      481 gtcgagccca cggtctttgt cgatgtcacg cccgatatgc gcattgcccg ggaggaagtg
      541 ttcggccccg tgctttcagt gctgcgctgg tccgacgaaa cggctttgtt cgaagcgatc
      601 aacagcgttg aatacggact gacgggatcg gtctggacaa aagatctcgc gaccgcgcat
      661 cgcgcagccg cacgcatcga gtcaggctat atctgggtaa atggcgcggg gccgcattat
      721 ctcagcgtgc ctttcggcgg ctacaaaatg tcgggactcg gcagagaaga atatatcgac
      781 gaactcttcc acttcaccga aaccaagaac atccacatca cgctctagcg acatgcgttc
      841 ccgccaggcg cactttcgaa cgcgcgctaa agcgccagca aattctgtag cgtctctcgc
      901 gtcgaaagat cggctttgcc atcgacgcgt tgcggccgga aatggcgctg gaatgccgtc
      961 accactttct cggttttgtc gtcataaaca ccggtgacat caagcccgta accgtaggcc
     1021 accaggttac gctgaaggtc ccaaacctac gcgccttcgt caccgagact caatcccata
     1081 tcggacccta acggatacgg cggcaaataa tggccgatac ccgccgccgc gagcctcacc
     1141 cagggaaaaa gctcacccgg atcggccttg cgctccgggg caatatcgga atgcgcgaga
     1201 atgttctgcg gcgggatttc atttcgttcg acgatgtcgc gacagagcgc gatcacggca
     1261 tcgatttgcg catcaggata aggaggcagc ccgaaatcgt gaccgccgtt gacgatctcg
     1321 atgccgatcg aaaccgcatt catatcggtt tcatccgccc agaaactttt gccggcgtgc
     1381 caggcgcggt aggcttcatg cacaagctga aatatgcggc cgtcttcctc gacgacgtaa
     1441 tgcgccgaga cttccgaatt cggatcgccc agtcgttcaa atgccgcggc gccggtcgtc
     1501 atccccgtat aatgcaaaac gatcgtatcg ggctcacgcc catgccttaa cccatagttg
     1561 ggcgaag
//
LOCUS       DASWOG010000229         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_41664,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000229 DASWOG010000000
VERSION     DASWOG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41664"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGB93_04715"
     CDS             complement(<1..297)
                     /locus_tag="VGB93_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY0145446.1"
                     /translation="MTVNRALTPHLRRLEAESIFIMREVVAEFAKPVMLYSIGKDSGV
                     MLHLAQKAFYPGKLPFPLLHVDTTWKFKEMIAFRDAMATQLGVELLVDTNQDGLR"
     gene            complement(385..930)
                     /gene="cobU"
                     /locus_tag="VGB93_04720"
     CDS             complement(385..930)
                     /gene="cobU"
                     /locus_tag="VGB93_04720"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY0145447.1"
                     /translation="MAGEIRSLLVLGGARSGKSRYAQTLVEQSGKTHVFIATAQPGDG
                     EMAARIAAHKDERGAHWRTVEAPLLLDAALRQVAAPDTIVLVDCLTLWLSNLMFASCD
                     VVSESLRLTETIRSLPGPAVFVSNEVGTGIVPDNRLAREFRDAQGRLNQIAAAACDMV
                     VFVAAGLPLTLKPGSHNAPML"
     gene            989..2083
                     /locus_tag="VGB93_04725"
     CDS             989..2083
                     /locus_tag="VGB93_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0145448.1"
                     /translation="MKPRRLAFTILLFAALAGAKPACAESQVNILGWADYVDAHVLED
                     FTKETGIKIVYDTFDSQTALESQLANGNGQYDVVMPSATSLHSLIREGLLQQLDMSRL
                     PNAKNLWPQIMAQLARDDPGNRYAVNYMWYTAGIAYDADKAKERLGDVAPDSWDIVFQ
                     PDNLAKFADCGVDVLDDPHTMFALALIYLKRNPATRNFNDLRRAADLLAGLHRNITRF
                     TSTDYVSALGNGDICLAVGWSGDASQARRGADEADNGTELGDATPKEGSVLGLDNLAI
                     PRDAPHLAAAYVFLDYLMRPDIAARNSEATGFASGVLAAKALLPHDFASNKAIYPDDD
                     TMSRLYALTNPDPMVDRYITRVWSLIKAAK"
     gene            2207..>3360
                     /gene="zapE"
                     /locus_tag="VGB93_04730"
     CDS             2207..>3360
                     /gene="zapE"
                     /locus_tag="VGB93_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY0145449.1"
                     /translation="MPNILLERYSERVKRGEFERDPAQEQVLEQLQALSTQLAFYRPT
                     RKSAALGWLVGARQKAAPLFGIYIWGSVGRGKTALMDMFFEETELSHKRRTHFHAFIA
                     DVHARIFAVRQQIKDGRVKGDDPIGPVAEALARESWLLCLDEFTVTDIADAMILARLF
                     KALLQAGVIVVATSNVPPEDLYKDGLNRDLFLPFIDILDAHMTVMRLDARTDFRLEKL
                     KGEPVYHIPADDRAHAALTRAFAALTGVEKASGKNLEFLGRSLRVPQAKNRVARFHFA
                     DLCAAALGARDFLAIAENFHTVIVDDIPVLDNTQVNEAKRFILLIDALYDQRVKLIVS
                     AAAEPQELYLAKAGREAFEFARTVSRLTEMRSAAYLASPHGRFGPGTQSGL"
BASE COUNT          697 a         1054 c          962 g          647 t
ORIGIN      
        1 ccgcaagccg tcttgatttg tgtcaacgag gagttcgacg ccgagctgtg tcgccatcgc
       61 atcgcgaaag gcgatcattt ccttgaactt ccaggtcgta tcgacgtgaa gcaagggaaa
      121 cggcagcttg cccggataaa atgccttctg cgccagatga agcatgacgc cggaatcctt
      181 gccgatcgag taaagcatga ccggcttggc aaattccgcg acgacctcgc gcatgatgaa
      241 gatggattcg gcctcgaggc ggcgcagatg cggcgtcaaa gctctattta cagtcatcat
      301 aagttatata ccgaattcta caatttggac tgctcataag ccccacaggt aggcggcgca
      361 acgccgattt gcgagccttt tagtctacaa cattggtgca ttgtgtgagc cgggcttgag
      421 ggtgagcggc aggccggcgg cgacgaacac caccatgtca catgccgcgg cggctatctg
      481 gttgagccgc ccctgcgcgt cgcgaaattc ccgcgcaagc cggttatccg gcacaatccc
      541 ggtgccaacc tcgttggaga cgaaaaccgc cggcccggga agcgagcgta tcgtctcggt
      601 gaggcgaagg ctctcagata ccacatcgca actggcgaac atgaggttcg aaagccaaag
      661 cgtcaggcaa tcgacgagca cgatcgtgtc cggcgcggcc acctggcgca aggccgcgtc
      721 cagcagcaat ggcgcctcga ccgtgcgcca gtgcgcaccc cgttcgtcct tatgcgcggc
      781 aattcgcgcc gccatttcgc cgtcgccagg ttgggccgtc gcgatgaaga cgtgcgtctt
      841 gcccgattgc tcgaccaatg tctgcgcgta ccggcttttg ccgctgcggg cgccgccaag
      901 cacgagcaga gagcgaattt cacctgccat tcaatcccat cccgcccgag gcttgacaaa
      961 ggccgcccgg cgccgcgaca ccaagcaaat gaaaccccgc cgccttgctt ttacgatctt
     1021 gctgtttgca gcactggctg gtgcgaagcc cgcctgcgcc gaaagccagg tcaatattct
     1081 cggttgggcg gattatgtcg atgcccatgt gcttgaggat ttcaccaagg aaacgggcat
     1141 caaaattgtc tacgacacat tcgattctca aaccgcgctc gaaagccagc tcgcgaacgg
     1201 caacggccaa tacgatgtcg tcatgccctc tgcgacgtca ctgcacagcc tgatccgcga
     1261 ggggctttta caacagctcg acatgagccg cctgccaaat gccaaaaatc tttggccgca
     1321 gattatggcg cagctcgccc gcgacgatcc gggcaatcga tatgccgtca attacatgtg
     1381 gtacacggcg ggcatcgcct acgatgccga caaggcgaaa gagcggctag gcgatgtggc
     1441 gccggactcc tgggacatcg tctttcaacc ggataatctg gcgaaattcg ccgattgcgg
     1501 cgtcgatgtt ctggacgacc cgcacacgat gttcgcgctc gcactgatct atctgaagcg
     1561 caatccggca acgcgaaact tcaacgacct gcgccgcgcc gccgaccttc tcgccggcct
     1621 tcatcgcaac atcacccgct ttacatcgac cgattatgtg agcgcactgg ggaacggcga
     1681 catttgcctc gccgtgggct ggtcgggcga cgcgtcgcaa gcgcgccgcg gggctgatga
     1741 agcggacaac ggcaccgaac ttggcgacgc gacgccgaag gaaggcagcg tgctgggcct
     1801 cgacaatctg gcgatcccca gggatgcgcc gcatcttgcc gccgcctatg tctttctcga
     1861 ttatctcatg cggcccgaca ttgccgcgcg caacagcgaa gcgacgggct ttgccagcgg
     1921 cgttctagcg gcgaaagccc tgcttccaca cgatttcgcc agcaacaagg cgatctatcc
     1981 cgacgacgac acgatgtccc ggctctacgc cctcaccaac ccggacccga tggtcgatcg
     2041 ctacatcacc cgtgtctggt cgctcatcaa ggcagcgaaa taggcggatc aaagaataca
     2101 gagcggtttg gccgcgtcgc aaaatcacac ttcaccgcgg cgacattgtt tgcacaatgg
     2161 ccgcactgaa cccaaactcg ccgcataaac atcgcccgga gtccgcgtgc cgaacattct
     2221 tcttgagcgt tattcggagc gcgtcaaacg cggcgaattc gagcgcgacc ccgcacaaga
     2281 gcaagtgctg gaacaattgc aggcgctttc gacgcaactc gccttttacc ggccgacacg
     2341 caagagcgcc gcgcttggct ggcttgtcgg cgcgcgtcaa aaagcggcgc cccttttcgg
     2401 catttatatc tggggctcgg tcgggcgcgg caagaccgcg ctcatggaca tgtttttcga
     2461 ggagaccgaa ctcagccaca aacggcgaac ccatttccat gccttcattg cggacgtgca
     2521 cgcgcgcatc ttcgccgtcc gccaacagat caaagacggc cgcgtgaaag gcgacgatcc
     2581 aatcggaccc gtcgcggaag cgctggcgcg cgagtcgtgg ctgttgtgtc tcgatgaatt
     2641 caccgtcacc gacatcgccg atgcgatgat cctggcgcgg ttgttcaagg cgctcctgca
     2701 agccggcgtt atcgttgtcg caacgtcgaa tgtcccgccg gaggacctct ataaggacgg
     2761 cttgaaccgc gatctgtttc tgccgttcat cgacatattg gacgcgcata tgacggtcat
     2821 gcggcttgat gcacgcaccg acttccggct cgaaaagcta aaaggcgaac cggtctacca
     2881 catccccgcc gacgacaggg cccacgcggc gctgacgcgg gcgttcgctg cgctcactgg
     2941 cgtcgaaaag gcaagcggta aaaatcttga atttctgggg cgcagcctgc gcgtgccgca
     3001 ggccaagaac cgcgtcgcac ggtttcactt cgccgacctt tgcgccgccg cgctcggggc
     3061 gcgcgatttc ctcgctatcg ccgaaaattt tcataccgtt atcgtcgatg acattccggt
     3121 gttggataat actcaggtga atgaggccaa acgcttcatt ctgctgatcg atgcgcttta
     3181 cgatcaacgc gtgaagctga tcgtctcggc cgctgcagaa ccgcaggaac tttatctcgc
     3241 caaggccggt cgcgaagctt tcgaattcgc ccgaactgtt tcgcggctca cggaaatgcg
     3301 ctcggcggct tatctggctt cgccgcatgg cagatttggt cccgggacgc agagcggcct
//
LOCUS       DASWOG010000230         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_5544, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000230 DASWOG010000000
VERSION     DASWOG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5544"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VGB93_04735"
     CDS             complement(<1..847)
                     /locus_tag="VGB93_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0145450.1"
                     /translation="MNKQSKQNIKRCVRWVINKVGLDVTFAELQQTGTVDLRALPHVL
                     PRILKLSGRHRARLTLAIIATLGATFVTLVIPHLLGVAVDQAHTLLSAGAGSYANARH
                     ALWVTATFLIAAATGRGLLTMISSYQCEWIGQKIAYDLRLAFFEKLQRLSFEYHDSVH
                     SGDLITRGMLDLEGMRMFIENGVQKALTLGLLLVVGAYLMFRTDPILAALAFSFVPFV
                     SWRAVRTGLFLRLTWTRLQQRMSILTRTIEENLQGVRVVRAFAAEPYELDKFDESAND
                     ALEMSN"
     gene            complement(872..1447)
                     /gene="msuE"
                     /locus_tag="VGB93_04740"
     CDS             complement(872..1447)
                     /gene="msuE"
                     /locus_tag="VGB93_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEY0145451.1"
                     /translation="MSGALTIPPKVVVFSANTHRPSKARFLATAIVEEVTAITPLDVR
                     FFDLADAGPGVGAFTRAELPPQALEIVEAIESADALIVSAPVHKGSYPGLFKHLIDFV
                     DLTTLINKPVLLAAKGGGTRHALVIEHQLRPLFGFFLAMTVPTAVYASDADFSDHVIV
                     DPVLKERIRIAASQFAVLLKAYERHFVTAAV"
     gene            complement(1459..1938)
                     /locus_tag="VGB93_04745"
     CDS             complement(1459..1938)
                     /locus_tag="VGB93_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0145452.1"
                     /translation="MAFALRKHDEDFGPAPMSDMNVTPLVDVMLVLLIVFMVAAPLMA
                     QGVPINLPKVQTKPLSDQKPPIGVSVDAAGQYYVDTKPVAAQQLVPTLDSEDPSKDRR
                     IHVRADANLPYGKVVEVMGLINSAGFSKVALVSQQPGAAAPASPATPAPPIPATPKP"
     gene            complement(1919..>2536)
                     /locus_tag="VGB93_04750"
     CDS             complement(1919..>2536)
                     /locus_tag="VGB93_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0145453.1"
                     /translation="WGWAIIVDKLIRLSVLNRRATKLIASVKSGLPVSTIAESFVGAS
                     EKDPFVAVYHAMVEENRRSADLIHTEAQTDSLQDRVHRVAQLASLGALEHLQNRLPNL
                     ATIGAVAPFVGLFGTVWGIMNSFQGIASSNNTSLSVVAPGIAEALFATALGLAAAIPA
                     VVFYNRISTDIGAYAKKLNTFIGVFEVELSRQLSRRGDRNGLRVA"
BASE COUNT          554 a          732 c          755 g          495 t
ORIGIN      
        1 gattggacat ttcgagcgca tcattggcag attcgtcaaa cttatcgagc tcataaggct
       61 cggccgcaaa ggcacggacc acgcgaacgc cttgcagatt ttcctcaatc gtgcgcgtta
      121 aaattgacat gcgctgctgg agtctggtcc atgtcagcct gagaaacaaa cctgtccgca
      181 cggcgcgcca gctaacaaaa ggtacaaagc tgaaagccaa agctgcgagg attgggtccg
      241 tgcggaacat cagataggcg cccacgacga gcaaaagccc tagcgtcaac gctttctgga
      301 ctccgttttc gataaacatg cgcatgccct cgagatcgag catgccgcgg gtaatcagat
      361 cgcccgagtg aacggaatcg tgatactcaa agctcagtct ctgcagcttt tcgaagaatg
      421 cgagccgcaa atcgtaggcg atcttctggc caatccactc gcattggtag ctcgagatca
      481 tcgtgagcag gccacgcccc gttgcagctg caatcagaaa cgtcgccgta acccaaagtg
      541 catgccgcgc gttcgcgtaa gatccggcgc cggcgctcaa tagcgtatgc gcctggtcaa
      601 ccgcgacgcc aagcaggtgg ggaattacga gagtgacaaa cgtcgcgccg agcgtcgcaa
      661 tgatcgcgag tgttaatcgc gccctgtggc gtccagaaag cttcagaata cgcggcagaa
      721 cgtggggcag cgccctgaga tcgactgttc ccgtctgttg cagttctgca aaagtaacgt
      781 ccagccctac tttgttaata acccagcgta cacatctctt gatgttttgc ttcgattgct
      841 tgttcacatg ccccgccaga aactttttta attacactgc tgcagtcaca aaatgccgct
      901 cgtatgcctt gaggagaact gcgaattgcg atgcggcgat tcgaattcgc tctttaagga
      961 ccggatcgac aatcacatgg tcgctaaaat cggcgtcgct ggcatagacg gcggtcggaa
     1021 cagtcatcgc caaaaagaag ccgaacaacg gccgcaactg atgctcgata accaaggcgt
     1081 gacgcgtgcc accgcccttg gcggcgagca gtacaggctt gttaatcaag gtcgtcagat
     1141 cgacgaagtc gatgagatgc ttgaatagtc ccggatatga gcccttgtgc accggggcgg
     1201 aaacgatgag tgcgtcggca ctctcgatcg cctcgacgat ttccagcgcc tgcggcggaa
     1261 gctcggcccg cgtaaaagca ccgacgcccg gcccggcgtc agccaagtca aagaaacgaa
     1321 cgtcgagcgg cgtgatcgcc gtcacctctt ccacgattgc cgtcgcgagg aatcgtgcct
     1381 tcgaggggcg atgcgtattg gcgctgaaga cgacgacctt cggcggtatt gtcagggctc
     1441 cggacatctg caaacctttc aaggcttcgg cgttgcggga atcggcggcg ccggtgtggc
     1501 tggcgaggct ggtgcggctg cgcctggctg ctgggagacg agggcgacct tgctgaagcc
     1561 ggcgctgttg atcaggccca tgacttcgac gaccttgccg taaggcaggt tcgcatcggc
     1621 gcgaacatgg atgcggcgat cctttgacgg atcctcgctg tcgagcgtcg ggacaagctg
     1681 ctgcgccgca accggcttgg tatcgacgta atattggccg gccgcatcga cgctgacgcc
     1741 gatcggcggc ttctgatcgc tgagcggctt ggtctgcacc ttgggcagat tgatcggcac
     1801 gccttgcgcc atcagcggtg ctgcgaccat gaacacgatg agaagaacga gcatcacgtc
     1861 gaccagcggc gtgacgttca tatcgctcat tggcgcaggg ccgaaatcct cgtcgtgttt
     1921 acgcaacgcg aaggccattg cgatctcctc tcctggaaag ctggcgcgag agctcgacct
     1981 cgaacacgcc gataaacgtg ttcagcttct tcgcgtaggc gccgatgtcc gtgctgatgc
     2041 gattatagaa aacgaccgcc ggaatagccg cggccagccc cagcgccgtc gcaaacaggg
     2101 cctcggcgat gccaggcgcc acgaccgaaa ggcttgtatt gttggaactg gcgatgccct
     2161 ggaacgagtt catgatgccc cacacggttc cgaagagacc gacaaagggg gcgacggcgc
     2221 cgatcgtcgc gagattggga agccggttct gcaaatgctc aagcgcaccc aaactggcga
     2281 gttgggcgac gcggtgtacg cgatcctgca agctatccgt ttgggcctca gtgtggatga
     2341 ggtcggccga acgccggttc tcctcgacca tcgcgtgata gactgcgacg aaaggatcct
     2401 tttccgaagc gccgacgaaa ctttcggcaa tcgtcgacac cggaaggccg gacttgacgc
     2461 tggcgatgag ttttgtcgcc cgtcggttca gcacgctgag ccgaatgagc ttgtcgacga
     2521 tgatcgccca gcccca
//
LOCUS       DASWOG010000231         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_188982,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000231 DASWOG010000000
VERSION     DASWOG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188982"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..585)
                     /gene="greA"
                     /locus_tag="VGB93_04755"
     CDS             complement(112..585)
                     /gene="greA"
                     /locus_tag="VGB93_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY0145454.1"
                     /translation="MEKVPMTAQGYSDLTEELRRRQQVERPRIIQAIAEARAHGDLSE
                     NAEYHSAKEAQSLNEGRVAELEDKLSRAEVIDVSKLSGDTVKFGATVTLVDEDTEEKK
                     VYRIVGESEADVKSGRVSITSPTARALIGKKVGDTVEVNTPGGGKSYEVLKVAFK"
     gene            complement(926..>3686)
                     /gene="carB"
                     /locus_tag="VGB93_04760"
     CDS             complement(926..>3686)
                     /gene="carB"
                     /locus_tag="VGB93_04760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0145455.1"
                     /translation="NKQEFIEIIERGIDASPTSEVLVEESVLGWKEFEMEVVRDKADN
                     CIIICSIENIDPMGIHTGDSMTVAPALTLTDKEYQIMRDASIAVLREIGVETGGSNVQ
                     FAVDPANGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEIANDITGGA
                     TPASFEPTIDYIVTKIPRFAFEKFPGAEPLLTTAMKSVGEAMAIGRTFAESLQKALRS
                     LETGLTGLDEVEIEGIELDDRHKVLRAALGRPTPDRLLVVAQALRYGMSLDEIHDACK
                     IDRWFIARVKEIVDLEDKVRSLGLPQDSENLRMLKAAGFSDMRLATLTGTDEASVRAL
                     RHELDVRPVFKRIDTSAAEFASPTAYMYSTYETPFAGVLANEALPSAREKIVILGGGP
                     NRIGQGIEFDYCCCHASFALRAAGYETIMINCNPETVSTDYDTSDRLYFEPLTAEDVL
                     EILDVERSAGRLRGAIVQFGGQTPLKLASTLQQAEIPILGTSVDSIDLAEDRDRFKRL
                     LDKLGLKQPMNGIAYSVEQSRLVAADLGLPLVVRPSYVLGGRAMAIIRDATTFDDYLL
                     DTLPGLVPSDIKARYPNDKTGQINTVLGKNPLLFDRYLSDAVEIDVDALCDGKDVFIC
                     GIMEHIEEAGIHSGDSACSLPPHSLAPDMIVRLEDETRKLALALNVGGLMNVQYALKD
                     DEIYVLEVNPRASRTVPFVAKVVGIPIAKIAARVMAGESLASFHLVSKPLGHVGVKEA
                     VFPFARFPGVDTVLGPEMRSTGEVIGLDRSFAIAFAKSQLGGGTKVPVSGTVFVSLRD
                     NDKPRIVPAAKKLLGIGFKFCATGGTARFLESQGIPTQKVNKVSEGRPHIVDAIKNGG
                     VQLVFNTTEGAQALADSRSLRRAALLHKVPYYTTLAGAMAASEAIVGYKAGDLEVKAL
                     QDYFA"
BASE COUNT          699 a         1132 c         1121 g          734 t
ORIGIN      
        1 aattcagccg cgaatatgcg cgcatgttcg gcgcgccgcc cgggcgcgat gcgcagcgtc
       61 tgcgcggcgt cgaatctcta caggacgata tgattgtgag cgcttaagcg cttacttgaa
      121 agctaccttc agcacttcgt agctcttgcc gccgccaggt gtattgacct cgacggtgtc
      181 gccgaccttc ttgccgataa gggcgcgggc cgtcggtgac gtgatcgaca cgcggccgga
      241 cttcacatcc gcctcgctct cgccgacgat ccgatagact ttcttttctt cggtatcctc
      301 atcgacgagc gtcaccgtgg cgccaaattt gaccgtgtcg ccggacagtt tcgagacgtc
      361 gatcacctcg gcgcgggaaa gcttgtcctc aagctcggcg acccggccct cattcaacga
      421 ttgagcttct ttggcggaat gatattcggc gttttccgaa aggtcgccat gcgcgcgggc
      481 ctccgcgatc gcctgaatga tacgcggccg ttccacctgc tggcgccgcc gcaactcctc
      541 cgtcagatcg gaatagcctt gcgcggtcat cggaactttt tccattctct caatctcttc
      601 ttggaccggc ggtcatcgcc gttgttgaac ccaaaatatt ccgcctgcct acgcggacag
      661 catcccgggc gggacgcagc ctcgcgtgcc cgctgctcat gcttccaaaa acagtatctt
      721 cgccgttcgg ctattccggc acggcgacgg atcagacagt ttaatgcatt cttcccgcga
      781 aacgcgacgg tttcaattgc aaatgcaaag ggaagcacac gtcgcaggtg gccgtgaccg
      841 aacgtcgcgg cggcgccacg acggatggag cgttccgccg tctgcagcca ttatattcca
      901 ataagttagg ccctcgcggc cggcctcagg cgaaataatc ctgcagcgcc ttgacctcca
      961 gatcgccggc tttgtacccg acgatcgcct cgctggcggc catggcgccg gcgagcgtcg
     1021 tataataggg cactttgtga agcagcgccg cacggcggag cgagcgcgaa tcggccaagg
     1081 cctgcgcccc ttccgtcgtg ttgaagacga gctggacgcc gccgttcttg atcgcatcga
     1141 cgatgtgcgg acggccctcg gaaaccttgt tgaccttctg cgtcgggatg ccctggcttt
     1201 cgaggaagcg ggcggtgccg cccgtcgcgc agaatttgaa gccaattccg agcaatttct
     1261 tcgccgccgg cacgatgcgc ggcttgtcgt tatcgcgcag cgagacgaag acagtgccgg
     1321 acactggcac tttggtgccg ccgccgagct ggcttttggc gaaggcgatg gcaaacgaac
     1381 gatcgaggcc gatgacctcg ccggtcgaac gcatttccgg cccgagcacg gtatcgacac
     1441 cggggaaccg cgcgaaagga aacaccgcct ccttcacccc gacgtggccg agcggcttcg
     1501 aaacgagatg gaagctcgcg aggctttcgc cggccatcac ccgcgccgcg atcttggcga
     1561 tcggaatgcc aaccaccttg gcaacgaacg gcaccgtgcg ggaggcacgg ggattgacct
     1621 caaggacgta gatctcgtca tccttgagtg cgtattgcac gttcatcagg ccgccgacat
     1681 tgagcgccag cgcgagcttc ctggtctcgt cttcgaggcg cacgatcatg tccggcgcga
     1741 gcgaatgcgg cggcagcgaa caggcgctgt cgcccgaatg gatgcccgcc tcctcgatat
     1801 gctccatgat gccgcagatg aagacatcct tgccgtcgca gagcgcatcg acatcgatct
     1861 cgactgcatc ggagagataa cggtcgaaga gcagcggatt tttgccgagt accgtgttga
     1921 tctgcccggt cttatcattg ggatagcgcg ccttgatgtc cgacggcacg aggcccggca
     1981 gcgtatcgag cagataatcg tcgaatgtcg tcgcatcgcg aatgatcgcc atggcgcggc
     2041 cgcccagcac ataggacggg cgcacgacaa gcggcaggcc gagatcggcg gcgacgagac
     2101 gcgattgctc gaccgaatat gcgatcccat tcatcggctg tttcagtccc agcttgtcga
     2161 gcaggcgctt gaagcggtca cggtcctcgg caagatcgat cgaatcgaca ctggtgccga
     2221 ggatcggaat ttccgcctgc tgcaaagtgg aggcgagttt caaaggcgtc tggccgccga
     2281 actgcacgat ggcgccgcgc agccggccgg ccgaacgctc aacgtctaaa atctccagca
     2341 cgtcttcagc ggtcagcggc tcgaaataaa gccgatcgga cgtgtcgtaa tccgtcgaga
     2401 cggtctcggg attacaattg atcatgatgg tttcatagcc cgccgcgcgg agcgcgaagc
     2461 tcgcatggca gcagcaataa tcgaattcga tgccctggcc gatgcggttc gggccgccgc
     2521 cgaggatgac gatcttctcg cgtgccgacg gaagcgcctc attggcgagg acgccggcaa
     2581 acggcgtctc atatgtcgaa tacatatagg cggtaggcga ggcgaattcg gcggcgctcg
     2641 tgtcgatgcg cttgaacacg ggtcgcacgt cgagctcatg gcgtagcgcc cgcacgctgg
     2701 cttcgtccgt accagtgagc gttgcgagcc gcatgtcgga aaagcctgcg gctttcagca
     2761 tccgcaaatt ttcggaatct tgcggcaggc cgagacttct caccttgtct tcgagatcga
     2821 cgatctcctt gacgcgggcg atgaaccagc ggtcgatctt gcaggcgtca tgaatttcgt
     2881 cgagcgacat gccgtagcgc agcgcctgcg ccacgacgag aaggcgatcc ggtgtcggcc
     2941 gcccgagcgc tgcgcgcaga accttatgcc tgtcatcaag ctcgatgcct tcaatctcga
     3001 cttcatcgag accagtgagg ccggtttcga gcgagcgcag cgctttctgc aaactctccg
     3061 cgaatgtgcg gccgatcgcc atggcttcgc cgacggattt catggccgtc gtcaggagcg
     3121 gctcggcgcc ggggaatttc tcgaaggcga aacgcggaat tttggtgacg atataatcga
     3181 tcgtcggctc aaacgaggcg ggcgttgcgc caccggtgat gtcgttggcg atctcgtcga
     3241 gcgtgtagcc gacggcgagt ttcgccgcga ccttggcgat cggaaagccg gtggctttcg
     3301 aggccaatgc cgaagaccgc gagacgcgcg gattcatttc gatcacgacg aggcgaccat
     3361 tcgcgggatc gacggcgaat tggacgttcg aaccgcccgt ctcgacgccg atctcgcgca
     3421 aaaccgcgat cgaggcgtcg cgcatgatct gatattcttt gtcggtgagc gtcagggccg
     3481 gcgcgacagt catcgaatcg ccggtgtgga tgcccatcgg atcgatgttc tcgatcgaac
     3541 agataatgat gcaattgtcc gccttgtcgc ggaccacttc catctcgaac tctttccagc
     3601 cgagcacgct ttcctcgacc agcacttcgc tggtcggcga ggcgtcgatg ccgcgctcga
     3661 tgatctcgat gaattcctgc ttgtta
//
LOCUS       DASWOG010000232         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_151899,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000232 DASWOG010000000
VERSION     DASWOG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151899"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..347
                     /locus_tag="VGB93_04765"
     CDS             114..347
                     /locus_tag="VGB93_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY0145456.1"
                     /translation="MPLGTHTTSMPVQVNADKCIAGKGCTVCVDVCPLDVLRIDELKG
                     LAYMRYDECWYCLPCEVDCPTQAITVNIPYLLR"
     gene            438..1304
                     /locus_tag="VGB93_04770"
     CDS             438..1304
                     /locus_tag="VGB93_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0145457.1"
                     /translation="MAELADLGDNSAVPAVVAALKDDDPGVREAAARALDAFDGAQAA
                     SALVGALADPEPSVRCAATEVLADRKDRAAAPLLIDLLAHPEAHVRAAALSALVGLRD
                     PAALTPALAALHDPDAEVRKNAAEVLGWLRAPEATPGLVAASRDENPEVRRAAIAALA
                     FAGGAAQAALVAAVSDSDWQVRESAASVLGKLVLPESVQALIAAMADDIWQVRVKAAA
                     SLGKLRDPSAIPALGAALDHPISNLRKEATAALGEIGDGTALPFLVAHADDPDPDVRK
                     LNRWATSKVTAL"
     gene            complement(1305..2165)
                     /locus_tag="VGB93_04775"
     CDS             complement(1305..2165)
                     /locus_tag="VGB93_04775"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145458.1"
                     /translation="MTESTSAQPGEIIIRNVSKSYGHGPFATPVIEDCSFTIEPNKFT
                     VMIGPSGAGKSTLIRLIAGFEKPTHGEILAGGTPVTGPGKDRLVVFQETALFPWMTTA
                     KNVLYGPQARGEANAATKILIDFLLDKVGLRAFRDRYPAQLSGGMQRRAELARALVNN
                     PAVMILDEPFRGLDAMTKKLMVDYLAKLCEDEKRTNFFVTTDIDEAIFLADRLIVMSA
                     CPTKVRAIIEVDLSRPRDYAAIFRDDRANAIKLEALSLLHEEAMKAFAGGSKAAADFI
                     EAYSRRSATV"
     gene            complement(2168..3034)
                     /locus_tag="VGB93_04780"
     CDS             complement(2168..3034)
                     /locus_tag="VGB93_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0145459.1"
                     /translation="MSADIANTVTGDAATRSVRARPSNNPFLALGTRRALISIAVALA
                     IWEIGSTSRVWLGHALPILGAVPPPVSVAVAWSQLVRDPGYWQSWYWSFLRVLLGFSA
                     ATLVGVPFGLFLAISKTFRGIAFPVFELLRPIPPLAWVPASIIFWPTQELSIDFVIFL
                     GAFYTIVLNVLGGARSIDLRLIQAARSMGATRWNIFRRILLPATLPSICTGMEVGIGI
                     TWEVVVAAEMISGGGASSGGGLGFFIWNSYIGGSYPDIIVGMFSIGIAGYLSSVAVRL
                     IEYRMTPWRRLR"
     gene            complement(3031..3879)
                     /locus_tag="VGB93_04785"
     CDS             complement(3031..3879)
                     /locus_tag="VGB93_04785"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145460.1"
                     /translation="MSLATISPAPRGALRIDNAVKLYDPDGARVLAIDHCSFDVAAGE
                     ICMIVGPSGCGKTTLLNAIAGFHGLDGGAIYLDNKLLCGPGQPQAPVGSDRIVVFQNG
                     ALFPWKTNLENIAFGPLVQKKLSRVEAFEKARYLMAEAGLEGHEDEYPGEISSGLRRR
                     VEIVRALINDPTVLLLDEPYRALDALTKSVMHEALLETHRRTGITIFFITHDLEEAIF
                     LGHRVIVMTARPCRPKRIIDINIPYPRNYTVLTSPRFRELAADISAAVDEEAVKSFEL
                     GEAEGQ"
     gene            complement(3882..>4561)
                     /locus_tag="VGB93_04790"
     CDS             complement(3882..>4561)
                     /locus_tag="VGB93_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0145461.1"
                     /translation="QVLQEWLSRDPTYGVSIYTPVYYQHILVSVQRIAIAFTLATTLG
                     VPFGLLLGWSRAFKDYLFPLFEIVRPIPILAWVPLAIIMFPGSELPVIFLTFLASFYA
                     TALNTMLGVKSIDKSYVLAASCLGASKLQIFSHVIVPGAMPFIFTGLQISVGVAWFSL
                     VAAEMVSGQFGLGYLINTSYTTVEYPTIVIAMLTLGIVGYLTSALVRVAGEWAMRARA
                     RQLAMEA"
BASE COUNT          914 a         1329 c         1487 g          831 t
ORIGIN      
        1 tcgcgcaaac ttccggtcgc gccgtacatc gtgccggtgc ctgaagccga tcgcgcgtct
       61 tacaacaaca tgcggctgaa atcggccgct ttggctgcgg aataggagtc atcatgccgc
      121 ttggcacgca tacgaccagc atgccggttc aggtcaatgc cgacaaatgt atcgccggaa
      181 agggctgcac ggtctgcgtt gatgtctgtc cgctcgacgt tctacggatc gacgaattga
      241 agggtttggc ctacatgcgc tacgacgaat gctggtattg cctgccgtgc gaggtcgatt
      301 gcccgacgca ggcgatcacg gtcaatattc cttatctgtt gcggtgacgt atgggtctgt
      361 tcgaggatca gatcggtgat cttgacgatt tgctcgaaaa gcttgccgcg ccggacgcct
      421 gagcgcggcg cgtcgccttg gccgagcttg ccgatctcgg cgacaattcc gcggtgcccg
      481 ctgttgtcgc ggcgctgaag gatgatgatc ccggtgttcg ggaggctgcg gcgcgcgcgc
      541 tcgatgcttt cgatggcgcg caagccgcgt cggcgctcgt tggcgcgctg gccgatcccg
      601 aaccttccgt gcgttgcgcg gcgacggagg tccttgccga tagaaaggat cgcgccgcgg
      661 caccgctgct gatcgatctg ttggcgcatc cggaagcgca tgtacgggcg gcggcgcttt
      721 ccgcactagt aggcttgcgc gacccagcgg cgctgacccc ggctctggca gcgctgcatg
      781 atcctgatgc cgaagtccgc aaaaatgcgg ccgaggtgct cggatggctg cgcgccccgg
      841 aggcaacgcc aggtcttgtt gcggcatcgc gtgatgaaaa cccggaggtt cgacgcgcgg
      901 cgattgccgc gctcgctttc gctggcggtg ctgcgcaggc cgctcttgtc gccgctgtaa
      961 gcgattccga ttggcaggtg cgtgagagcg cggcgagcgt cttgggtaag cttgtgttgc
     1021 ccgaaagcgt tcaggcgctg atcgcggcga tggccgacga catctggcaa gtgcgcgtca
     1081 aggccgcggc aagtctcggc aaattgcgcg atccctcagc cattccggcg ctcggcgcgg
     1141 cgctcgatca tccgatcagc aatctgcgca aggaggcgac ggcagcactg ggcgagatcg
     1201 gcgacgggac ggcgctacca tttcttgtcg cgcacgcaga tgatcccgat ccggatgtgc
     1261 gcaaactcaa tcgctgggcg acgtccaaag tcaccgcgct ctgatcatac cgtcgcgctt
     1321 cggcggctgt aggcctcgat aaaatccgcc gccgccttgc tgccgccggc gaacgctttc
     1381 atcgcctctt cgtggagaag cgataaggct tcgagcttga tggcgttggc gcgatcgtcg
     1441 cggaaaatcg cggcgtaatc gcgcgggcgt gacagatcga cctcgataat cgcgcggacc
     1501 ttcgtgggac aagcgctcat gacgatcagc cgatcggcaa ggaaaatcgc ctcgtcgatg
     1561 tccgtcgtga cgaagaagtt cgtgcgcttt tcgtcctcac agagcttggc caaataatcg
     1621 accatcagct ttttcgtcat cgcgtcgagc ccgcggaagg gttcatcgag gatcatgacc
     1681 gcgggattgt tgacgagcgc gcgggcgagt tcggcgcggc gttgcatgcc gccggaaagc
     1741 tgcgccggat agcgatcacg gaacgcgcgc aggcccacct tgtcgaggag gaaatcgatg
     1801 aggattttcg tcgcggcgtt ggcctcgccg cgcgcttgcg gcccgtagag aacattcttc
     1861 gcagtcgtca tccagggaaa caacgccgtt tcctgaaaga caacgaggcg gtccttgccg
     1921 ggcccggtga caggcgtgcc gcccgcaagg atttcgccgt gcgtcggctt ctcgaatccc
     1981 gcgatgaggc ggatcaatgt gcttttgccg gcgccggatg ggccgatcat cactgtgaat
     2041 ttgttaggct cgatggtgaa agaacaatcc tcaatgacgg gcgtcgcgaa cgggccgtga
     2101 ccgtaagatt tgctcacatt gcggatgatg atctcgcccg gctgtgcgga tgtggattcg
     2161 gtcatgctta gcgcagcctc cgccaagggg tcatgcgata ttcgataagc cgtaccgcga
     2221 cgctcgacag atatccggcg atgccgatcg agaacatgcc gacgatgatg tcgggatagg
     2281 agccgccgat ataggagttc caaatgaaga agcccaatcc gccgcccgaa gacgcgccgc
     2341 cgccggaaat catctcggcg gcgacgacca cctcccaggt gattccgatg cccacttcca
     2401 taccggtgca gatcgaaggc agggtggcgg gaagcagaat tcggcggaaa atattccagc
     2461 gcgttgcgcc catcgagcgc gcggcctgaa tcaggcgcag atcgatcgac ctggccccgc
     2521 cgagcacatt aagcacaatc gtatagaagg cgccgaggaa gatgacgaaa tcgatcgaaa
     2581 gttcctgcgt cggccagaag atgatcgagg cgggcaccca ggcgagcggc ggaattgggc
     2641 gcagcaattc gaagaccggg aatgcaatgc cgcgaaaggt cttgctgatg gcgagaaaca
     2701 gaccaaaggg aacgccaaca agcgtggccg ccgagaagcc gagcagcacg cgcagaaagc
     2761 tccaatacca gctttgccaa tagccgggat cgcgcacgag ttgcgaccat gcgacggcga
     2821 ccgagaccgg cggcgggaca gcgcccagaa tgggcaatgc gtggccgagc cagacacggc
     2881 ttgtcgagcc gatctcccaa atggcgagcg caacagcgat cgagatgagc gcccgccgcg
     2941 ttccgagcgc gagaaaggga ttattcgaag gcctcgcgcg gacgctgcgc gttgcggcgt
     3001 cccccgtcac ggtgttggca atgtcggcgc tcattggccc tccgcctcgc cgagttcgaa
     3061 ggattttacg gcttcctcat cgaccgccgc ggaaatgtcg gcggcgagtt cgcgaaagcg
     3121 cgggctcgtc agcacggtgt agttacgcgg ataggggata ttgatgtcga tgatccgctt
     3181 tggccggcac ggccgcgctg tcatgacgat gacccggtgg ccgaggaaga tcgcttcttc
     3241 aagatcgtgc gtaatgaaga aaatcgttat gccggtccgc cggtgtgttt cgagcaaggc
     3301 ttcgtgcatt accgatttag tgagcgcgtc gagcgcgcga taaggctcgt cgagcagcag
     3361 gacggttgga tcgttgatca gcgcgcgaac gatctcgacg cggcgccgca ggccggagga
     3421 aatttcaccg gggtattcgt cttcgtgacc ttcgagcccg gcttcggcca tcagatagcg
     3481 cgccttttcg aaagcctcaa cgcggctgag ctttttttgg accagcgggc cgaacgcgat
     3541 attttcgaga ttggtcttcc acgggaacaa tgcgccgttc tggaagacga cgatacggtc
     3601 ggacccgacg ggcgcctgtg gctgaccggg gccgcagagc aatttgttgt cgagatagat
     3661 agcgccgccg tcgaggccat gaaaaccggc aatcgcattc agcaacgtgg tcttgccaca
     3721 gcccgaaggg ccgacgatca tgcaaatctc gcctgccgcg acatcgaagc tgcaatgatc
     3781 gatggcgagc acgcgtgcgc cgtctggatc atagagcttc accgcattgt cgatcctgag
     3841 cgcgccgcgc ggcgcggggc tgatggtcgc gaggctcatg ctcaggcctc catcgcgagc
     3901 tggcgcgcac gcgcgcgcat cgcccactcg ccggcgacgc gaaccagcgc gctcgtgaga
     3961 tagcccacga tcccgagcgt cagcatggcg atcacgatcg tcgggtattc gaccgtcgtg
     4021 taggacgtgt tgatgagata gccgaggccg aattggcccg acaccatctc ggctgcgaca
     4081 agcgaaaacc aggcgacgcc gaccgagatt tgcaatccgg tgaagatgaa gggcatcgca
     4141 cccggcacga tcacatggct gaaaatttgt aattttgaag cgccgaggca actcgccgca
     4201 agcacgtagg atttgtcgat cgatttgacg ccgagcatcg tgttgagcgc cgtcgcatag
     4261 aaagaggcga ggaaggtcag gaagatcacc ggcagttccg agccggggaa catgatgatc
     4321 gcgagcggca cccaggcgag aatcgggatc ggccggacaa tctcgaacaa agggaaaaga
     4381 taatccttga aggcgcgcga ccagccgagt agcaggccga acggcacgcc gagcgtcgtc
     4441 gccagcgtga aggcgatcgc gatccgctgc acgctgacaa gaatgtgctg ataatagacg
     4501 ggcgtataaa tcgagacgcc ataggtcggg tcgcggctta gccattcctg cagaacctgc
     4561 g
//
LOCUS       DASWOG010000233         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_151926,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000233 DASWOG010000000
VERSION     DASWOG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151926"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGB93_04795"
     CDS             <1..336
                     /locus_tag="VGB93_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0145462.1"
                     /translation="HPKASTLGVDKGYGSGDFVNELRTMNVRPHIAQNISGRRSTIDR
                     RTTRHQAYSASQRIRKRIEEAFGWMKTIAGQRKTKFRGVDRVGWAFAFAAAAYNLVRL
                     PKLIEEMTP"
     gene            complement(466..1878)
                     /locus_tag="VGB93_04800"
     CDS             complement(466..1878)
                     /locus_tag="VGB93_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0145463.1"
                     /translation="MCGTVLGYVAGLVSVIGHVDRARPLRDYCTGLLMPVERKSVEPM
                     AAVTAPERTAAQHQSLLHFVGEGRWSDEAVLAKVQEMVLPKIEDHGPIQAWIIDDTGF
                     PKKGRHSVGVARQYCGQLGKQDNCQVAVSLSLANRHASLPVAYQLYLPHDWADDELRR
                     CKAHVPQEIGFKTKPEIALAQIRAAYDAGLPRGVVLMDAGYGANTELRRAIAALGLTY
                     VAGILPNTTVWAPGTAPARPKAWSGRGRPPSLMRRDSHHQPISVKALAQSLPAKAWRT
                     VTWREGTAEPLTSRFARLRVRAAHRDYNLSEARLEEWLLIEWPKGEAEPIKYWFSTLP
                     EKIAFTRLVDLTKLRWRIERDYQDLKQEIGLGHFEGRGWRGFHHHATLCIASYGFLIC
                     ERQTIPPSGMRPGGVFQTPCLSANQRPRGSAAAHRTSHPKLHRDHATTINGRARQTTR
                     AMSLLCTTDQESQATKFMTQ"
BASE COUNT          383 a          629 c          595 g          373 t
ORIGIN      
        1 catccaaaag ccagcacgct cggcgtcgac aagggttatg gctcgggcga tttcgtcaat
       61 gagctacgga ccatgaatgt gcgtccgcac atcgcgcaaa atatcagcgg ccggcgttca
      121 accatcgacc ggcgaacgac gcgccatcag gcctactcgg cgagccagcg tatccgcaaa
      181 cggatcgagg aggcctttgg ttggatgaag accatcgccg ggcagcgcaa gacgaagttt
      241 cgcggcgtcg atcgcgttgg ctgggccttc gccttcgccg ccgcagccta caacctcgtc
      301 agactaccga aattgattga ggagatgaca ccatgatgcc acctaaggcg tcgctcatca
      361 cagccacatc cgccgaccgt catcatcgac aaagccagtc tcggaaaatc agcaacaact
      421 ctgtccgcgc ccgaagcgcc gcaatttttc agcagcctgc tagtcttact gcgtcataaa
      481 tttcgtagcc tgactttctt gatcggtcgt gcacagcagg gacatcgcgc gagttgtctg
      541 gcgagcgcga ccgttaatcg tcgtcgcatg gtcgcgatgg agtttgggat gtgacgttct
      601 gtgcgcagcg gcagagccgc ggggtcgctg attggcggaa agacaaggcg tttggaagac
      661 cccgccggga cgcatgcccg aggggggaat cgtctgcctt tcgcagatca ggaatccata
      721 ggaggcaatg cacagcgtcg cgtgatgatg gaagccgcgc cagccgcggc cttcgaagtg
      781 ccccaggccg atctcctgct tgagatcctg ataatcgcgc tcgatgcgcc aacgcagctt
      841 tgtaaggtca accagacgcg tgaatgcgat tttctccggc agcgtcgaga accagtattt
      901 tatcggttca gcttcgccct tgggccactc gatcaatagc cattcttcaa gccgcgcctc
      961 ggacagattg taatcgcgat gggcggcacg aacgcgcagg cgtgcgaaac gcgaggtgag
     1021 cggctcggcg gttccttcgc gccatgtgac cgtgcgccag gctttggccg gcaggctctg
     1081 cgccagcgcc ttcaccgaga tcggttggtg gtggctgtcg cggcgcatca ggctgggtgg
     1141 gcggccgcgg cctgaccacg ccttgggacg agccggtgcg gtgcccggcg cccacactgt
     1201 ggtgttgggc agaatgccgg cgacataggt caagcccaag gcggcaatgg cccggcgcag
     1261 ctccgtgttg gcgccatagc cagcgtccat caggacgacg ccgcgaggca gtcccgcgtc
     1321 ataggccgcg cggatttgcg cgagcgcgat ctcgggcttg gtcttgaagc cgatctcctg
     1381 aggcacatgg gctttgcacc gccgcagttc gtcgtcggcc cagtcgtgcg gaagatagag
     1441 ctgataagct accggcaggc tggcgtgcct gttggccagc gacagcgaga cggcgacctg
     1501 acaattatcc tgcttgccca gctggccgca atattgccgc gccacgccga ccgaatgccg
     1561 gcctttcttg gggaaccctg tgtcatcgat gatccacgcc tggattggtc catggtcctc
     1621 gatcttcggc aggaccatct cctgcacctt ggccaaaacc gcctcatcgg accagcgtcc
     1681 ctcacccacg aagtgcaaca gggattggtg ctgtgccgcc gtccgttccg gcgctgtcac
     1741 cgccgccatc ggctcaacgc tcttgcgttc gaccggcatc agaagcccgg tgcaataatc
     1801 gcgtagcgga cgagctcggt ccacatgacc gatcacgctc accagccctg caacgtaacc
     1861 caaaaccgtt ccgcacagcc atccgttcga agatccatcc tggcctccgt gccgaaatga
     1921 atctcccaag cctacgtgag tcgcctccgc aaaccgccgc ggctttctga ctcagtaaga
//
LOCUS       DASWOG010000234         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_243617,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000234 DASWOG010000000
VERSION     DASWOG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243617"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGB93_04805"
     CDS             complement(<1..275)
                     /locus_tag="VGB93_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145464.1"
                     /translation="MIMKIPAQDGDMRTAMNCAWALAAGIVIIDCAWLPLAGFHAAPA
                     PLLRDAAIFAALCGLWGIYAFLRPDATLAALIETAAFLIIFTLALAL"
     gene            900..1151
                     /locus_tag="VGB93_04810"
     CDS             900..1151
                     /locus_tag="VGB93_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145465.1"
                     /translation="MSIADHFDHTPDAGFVRSYDTRSARRQFQVSLALVVVLGISAFA
                     LGLLVRFDPPAGVSQTPIAAHDVHIAGVMPGLLGFAPHT"
     gene            1318..1974
                     /locus_tag="VGB93_04815"
     CDS             1318..1974
                     /locus_tag="VGB93_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145466.1"
                     /translation="MSTPPAKSFGRRTWAESPTRAPAAKPRAAATNLPSNLPPDLVAA
                     LVRGSGPRPTTSAGHAAVISVAWSVRAAVLGGLIVGLINAAARATTFLSFGGLAGADK
                     LPLENTLLNQFAPEALGHASLGQATVPIAVLVALAGLWAGARASAFALLFAHKILARL
                     GQTSLVAYSLGGAAASLAYALIAALFWAETTPVSILIETLSGLGAGFFYRLFAPTERG
                     "
     gene            2016..>2258
                     /locus_tag="VGB93_04820"
     CDS             2016..>2258
                     /locus_tag="VGB93_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145467.1"
                     /translation="MPKPPRKASRSKAGKPELKPLGDHLASLLNPALTSTPPTGFAEA
                     PATYLAALGTSGIEATAASLKDLLERGDPNLLDKAPW"
BASE COUNT          437 a          710 c          657 g          454 t
ORIGIN      
        1 aacagcgcca gagccagcgt gaagatgatg agaaacgccg cggtctctat cagggcggcg
       61 agcgtcgcgt caggacgtaa aaaggcatag atgccccaga gcccgcacaa ggcggcgaag
      121 atcgcggcgt cccggagcaa tggtgccggg gcggcgtgaa agcctgccaa tggcaaccag
      181 gcgcaatcaa tgatgacgat cccggcggcg agcgcccagg cgcaattcat cgccgtccgc
      241 atatcgccat cttgggctgg aattttcatg atcattcgcg gctccgccgg ccggaagatg
      301 ccgcgggaat tggcaagcaa ctgtaaaatc ggacggacaa atcgggcggg tgatatttgt
      361 ggcgaatact taaagccgaa ggctcggatt taagcagacg tgttcaataa atttcagtcc
      421 tattggccaa aaatacttct ttgacgttgc cgtaactata cggcgatgca gtcagaaaaa
      481 ttttcatcaa ctttattgcc tgtctcgccc cacgatgcgc cggcggcctg caccggcacc
      541 gttagaaaat attaaatcat tgttttatat acgatttatc ctgcgcttgg gcgccgccgg
      601 cctcggcaac ggcggagcga tggaacgtaa aagacatcca aaggttgagc tgaaagaggc
      661 gaaaggggtc tcaaactgcg ctattcagac aactgtcaag aattttgatg cgcattttga
      721 aaccttttcc gtcgatgtgg cgttcgagag acgtacgaga tgtttactgc caagccagca
      781 aagatagtcg cgtcaagacc gcagccgggc tctccaatgt gcttttgcgc acaggcaaag
      841 aaaggggaac ccaataattg ttggtggtct tcgaggccgc gtgctgagag gagttaggaa
      901 tgtcgatcgc ggatcacttt gatcatacgc cggatgcagg tttcgtccgc agttacgata
      961 cgcgctccgc gcgtcgacag tttcaggttt cgctggcgct ggttgtcgtt cttggcatct
     1021 ccgccttcgc actgggtctt ttggtccgct tcgatccgcc ggctggcgtt tcgcagactc
     1081 cgattgcggc ccatgacgtt catattgccg gcgtaatgcc gggacttttg ggtttcgcgc
     1141 cgcacactta ggcggcgcaa agttaccacg acccccgatg ccccccgtag gaggtcgtgt
     1201 ctgagggccg cgctccctaa aaggacgcgg ccctttcttt ttgcctccag gcagaaaggg
     1261 gcgagtttcc ccgatcgcat caatcgtttc gtaggaggcg attgctaggc tgcggccatg
     1321 tcaacgccgc ccgcaaaatc attcggccgc cgcacctggg cagaaagccc gacgcgggcc
     1381 cccgccgcga agccgcgcgc cgctgctaca aacttgccat cgaacttgcc gccggatctc
     1441 gtcgcggctc tggttcgcgg ctccgggcct cgtcccacca cctccgcggg ccacgcagcc
     1501 gttatcagcg tggcctggtc ggtccgcgcc gccgttctcg gcgggctgat cgtcggcctt
     1561 atcaatgccg cggcccgtgc cacgaccttc ttgagttttg gcggcctcgc tggcgccgac
     1621 aagctgccgc tggaaaatac acttctcaat caattcgcgc ccgaagcact aggccacgca
     1681 tcgcttgggc aagccacagt gccgatcgcc gtgctcgtcg ccctagccgg gctctgggcc
     1741 ggcgcccgcg ccagcgcctt cgcgcttttg tttgcgcata aaatcctcgc gcgcctcggc
     1801 cagaccagcc tggttgccta tagtctcggc ggcgcggcgg cgtctctggc ctatgcgctc
     1861 atcgccgcgc tcttctgggc cgagacgacg cccgtctcga tcctcatcga aacactctcg
     1921 ggtctcggcg ccggcttttt ctatcggctt tttgcaccga ccgagcgcgg ctaattccgc
     1981 ttcacactcg ggcggtgcgc acccatattg ggcctatgcc caaaccaccc cgcaaagcca
     2041 gccgcagcaa ggccggaaag ccagaactga agccgctcgg cgaccatctg gcgtcgctgc
     2101 tgaacccggc gctgaccagc acgccgccga ccggctttgc cgaggcgccg gccacttacc
     2161 tcgccgctct cgggacaagc ggaattgaag ccaccgccgc gtcgctgaaa gacctgctcg
     2221 aacgcggcga tcccaatctg ctcgacaagg cgccgtgg
//
LOCUS       DASWOG010000235         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_41815,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000235 DASWOG010000000
VERSION     DASWOG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41815"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1051)
                     /locus_tag="VGB93_04825"
     CDS             complement(272..1051)
                     /locus_tag="VGB93_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145468.1"
                     /translation="MIDFLTTAPLWVEILLVVIVPTVLAMLGPVLIRRFVELEHLAIN
                     NEVAGFKFATIGVTYAVLLAFSIVVVWEKASDAESTVEKEAGAAATIYRLSDGLGAPK
                     STAVRTAMTKYLTSTINDEWPAMENGHANKPTWEALGGLYAAMLTPEAVDKSDVSIKS
                     EVFNQLSIITQARRARLMAADGVVPDPVWAIVFICAFLVIGFTFFFGTRNLRAQTLMT
                     GLLAFLVFSELVIVIAIERPFAGGVKEAPTPLVHVLRDFAS"
     gene            complement(1157..1972)
                     /locus_tag="VGB93_04830"
     CDS             complement(1157..1972)
                     /locus_tag="VGB93_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0145469.1"
                     /translation="MRRNPGEDEPDGLPAATALPIWLISACAALALHAGSIATAAYYM
                     KPAEVEDDLGAPAVEVGIEFAAPHLQPVDLPPGPPADDAVATPAVVAQKANVEETQLP
                     TDQPIESDDPDRLVTPDSSSKPKEDDPTIKAVEAMPSEESIASEAAAPPVADAAKESQ
                     QSTAPVEGTGESETRIAMTWERELGIHLNKHKRYPTGAKNRNVQVTLSFTIDRLGHLM
                     SANVVTSSGDPAFDQAAVAMMHNSDPVPAPPAVVADKGLTFTLPVIFKAHDRN"
     gene            complement(1974..2423)
                     /gene="exbD"
                     /locus_tag="VGB93_04835"
     CDS             complement(1974..2423)
                     /gene="exbD"
                     /locus_tag="VGB93_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEY0145470.1"
                     /translation="MGVTLADSDLDDDLEEAHEINVTPFIDVMLVLLIIFMVAAPLST
                     VDLPVDLPTSTADPLKRPDKPTYLTIKSDLSLALGETAVKRQDLIGAVDTAVNNAKEH
                     RIFLRADRTVPYGEMMSVLEILRRGGYLKVSLVALEGVPDVPSTNGK"
     gene            complement(2423..>2617)
                     /locus_tag="VGB93_04840"
     CDS             complement(2423..>2617)
                     /locus_tag="VGB93_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0145471.1"
                     /translation="AEALLATAIGLVTAIPAVIIYNHFTRSTKSYLELVARASGLLTR
                     LLSRDLERRHMGVPISRAAE"
BASE COUNT          520 a          768 c          793 g          536 t
ORIGIN      
        1 cagctggtta gagcgccggc ctgtcacgcc ggaggtcgcg ggttcgagcc ccgtctctcc
       61 cgccattaaa tcaatgattt agaggctatt agagtcgaaa cggttccaac ttttgtttgg
      121 aaccagtttc agcttctgaa ctcattttgt ttcgatgcga gcttgcagcg gcctatacgc
      181 cacgattcat cggcagcgcg gacagctgcc taaccggcca gatcgcccac gcgcagttca
      241 gcgtggcagc atcgctttcg ccacgcttgc gtcatgacgc gaagtccctc agcacatgaa
      301 cgagcggcgt cggcgcttct ttgacgccgc cagcgaacgg cctctcgatc gcgatgacga
      361 tgaccaattc ggaaaatacc agaaatgcca gcagcccggt catcagcgtt tgggcgcgca
      421 gatttctcgt tccgaagaag aaggtgaagc cgatgaccag aaacgcgcat ataaaaacga
      481 tcgcccaaac cggatcaggg acgacgccgt cagcggccat gagcctggcc cggcgtgcct
      541 gcgttatgat ggagagttgg ttgaagacct ccgacttgat tgacacatcg ctcttatcaa
      601 cggcttccgg cgtaagcatc gcggcataga gcccgccgag agcctcccat gtgggcttgt
      661 tggcatggcc attttccatc gccggccatt cgtcgttgat cgtggacgtg agatatttgg
      721 tcatggcggt ccgaacggcc gtactcttcg gcgcgccgag tccatcggac aagcgataga
      781 ttgtcgccgc ggcgccggct tctttttcga ccgttgattc cgcgtcgctg gccttctccc
      841 aaacgactac gatcgaaaag gcgagaagga cggcgtatgt cacgccgatg gtcgcaaact
      901 tgaagccggc gacttcgtta tttatggcca gatgttcgag ctctacgaaa cgtcggatca
      961 atacgggtcc gagcatcgcg agcacggtcg gcacgatgac cacgagcaaa atttcgaccc
     1021 aaagaggcgc ggtcgtcaga aaatcaatca tgtcaagcca cccaattcca agggaggcca
     1081 ataaagcacc gcttacgcta gctcggcaat tcccgaatgt atgtccattc gccggtccga
     1141 aaaagctcgc tcgcaactaa ttcctgtcgt gtgctttgaa aatgaccggc agcgtgaaag
     1201 tcagcccttt gtcggccacg actgcgggcg gggcagggac aggatcggaa ttgtgcatca
     1261 tcgccaccgc agcctgatcg aaagccggat cgccggaact cgtcacgaca ttcgctgaca
     1321 tgagatggcc gaggcggtcg atcgtaaagc tcaatgtcac ttgcacgttg cggttcttgg
     1381 cacccgtcgg atagcgtttg tgcttgttga gatggatgcc gagttcgcgc tcccaggtca
     1441 tcgcgatgcg cgtctcgctt tcgccggtgc cctcgaccgg cgcggtcgat tgctgggatt
     1501 ctttggcggc atcggcaacg ggaggcgccg cggcctcgga ggcaatcgat tcctcggatg
     1561 gcatcgcctc caccgccttg atcgtgggat cgtcttcttt gggcttcgac gaactatcgg
     1621 gagtcacaag gcgatccgga tcgtcactct caatcggctg gtctgtggga agctgcgtct
     1681 cctcgacgtt tgccttctgc gcgaccactg ccggcgttgc cacggcatcg tccgctggtg
     1741 ggccgggcgg cagatcgacg ggttgaagat gcggtgcggc gaattcgatc ccgacctcga
     1801 cagcaggggc gccgagatcg tcctcgacct cggccggttt catgtaatag gccgccgtcg
     1861 cgatcgaacc ggcatggagc gcgagcgccg cgcaggccga gatgagccag atcggcaacg
     1921 ccgtcgccgc cggcaggcca tcgggttcat cttcgccagg attacgacgc atcttacttg
     1981 ccgtttgtgg acggcacatc gggcacacct tcgagtgcga cgagcgaaac cttgaggtag
     2041 ccgccccggc gcaggatttc gagcacactc atcatttcac cgtaaggcac ggtgcggtcg
     2101 gcgcgcaaaa aaatgcgatg ttccttggcg ttattgactg cggtatcgac cgcgccgatc
     2161 aagtcttgcc gtttcaccgc cgtttcgccg agcgccaggc ttagatcgct tttaatggtg
     2221 agataagtcg gcttatccgg gcgcttcagc ggatccgcgg tcgatgtcgg cagatcgacc
     2281 ggcaggtcga ccgtcgacaa gggcgccgcg accatgaaga taatgagcag caccagcatc
     2341 acgtcgatga acggcgtgac gttgatctcg tgcgcttcct ccagatcgtc gtcgaggtcg
     2401 gaatcggcga gggtaacgcc cattattccg ccgcccggct gatcggcacg cccatatgcc
     2461 gtcgttcaag atcgcgcgac aaaagccgcg tcagcagccc cgaggcgcgg gcaacgagtt
     2521 cgagatagct tttggtcgac cgggtgaagt gattgtaaat gatgacggcg ggaatggcgg
     2581 tgacgaggcc gattgcggtg gccagcaggg cttcggc
//
LOCUS       DASWOG010000236         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_60415,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000236 DASWOG010000000
VERSION     DASWOG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60415"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGB93_04845"
     CDS             <1..663
                     /locus_tag="VGB93_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0145472.1"
                     /translation="GGKPAIDDHHIALITEMYQGDSLAAAVQEGFHVRDDVYRAISRE
                     SDVANRGAVSPKGFELSGRRIAQVMKGGYNLAFVDVGGWDTHVNQGGATGYLADRLGE
                     LGRGLVAFSDELGPQDWRDTVVVVISEFGRTFRENGDKGTDHGHGSTYWVLGGSVNGG
                     RFHGPQVKVEEASMFQNRDFQVLTDYRSLFGGLLGKMYGLNSAALNTIFPTAKPVDLA
                     LV"
     gene            829..>2451
                     /locus_tag="VGB93_04850"
     CDS             829..>2451
                     /locus_tag="VGB93_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0145473.1"
                     /translation="MTRSAWKAEPAGPRSVALVGPYGTGKSILFEALLASAGAAPKRP
                     SARAMTTELRLGHCTFMGEPWALIDCPGSVEFAQETAAALAVVDFAIVVCEPSADRLA
                     NLPFLLKTIEAAGLPHLIFINKIDALTGQVREIVAALQPHSQYPLVLRQIPIRKHDAV
                     TGYIDVVSERAYHYCREDSERCMLPSELLIPEQEALAGLTEVLADHDDALLEKVIEDI
                     APSPDEIFATLHKDQESGTIVQVLFGAAARGHGIFRLWKALRHDVPAASQTAQRRAIA
                     PSDQPLVQIFKTVHAGQAAYAGKLSYARIWRGGLRDGAPLNGGRVGGLYRFVNGEIAR
                     TQTADPGELVAIGRLEGAMTGAVIGEGSAELPFPHPPVAVYAFAIAAKDRKDDVKLSG
                     ALHKIVEEDPSLSIAHRRETGETLLCGQGEIHLNGTLERLASVYNCQIDVAPAKVAYK
                     ETIKRGVKQHARLKRQTGGHGQFADVVLEIAPRARGEGFSFVDKIVGGAVPRQYIPSV
                     GEAAEEAMHKGPLGYPVVDVEVILLAGSFHAVD"
BASE COUNT          453 a          740 c          776 g          482 t
ORIGIN      
        1 ggcggtaagc cggctatcga cgatcaccac atcgccctga tcaccgaaat gtatcagggc
       61 gatagccttg cggccgcggt gcaggaaggc tttcatgtcc gcgacgacgt ctatcgcgcc
      121 atctcgcgcg agagcgacgt cgccaatcgc ggcgccgttt cgccgaaggg cttcgagctg
      181 tccgggcgcc gcatcgccca agtgatgaag ggcggctata atctcgcctt cgtcgatgtc
      241 ggcggctggg acacacacgt caaccaaggc ggtgcgaccg gctatctcgc cgatcgtctc
      301 ggcgaactgg ggcgcggcct tgtcgctttc agcgacgagc taggcccgca agattggcgt
      361 gacacggtgg tcgtggtgat ctccgaattc ggccgcacct ttcgcgaaaa tggcgacaag
      421 ggcacggatc atgggcacgg cagcacctat tgggtgctcg gcggcagtgt gaacggtggc
      481 cgcttccacg gcccgcaggt gaaggtcgaa gaggctagca tgttccagaa tcgagatttt
      541 caggttttga cggattatcg cagtctgttc ggcggcttgc tcggcaagat gtatgggctc
      601 aattccgcgg cgctcaacac gatcttcccg acggcgaagc ctgttgatct cgcgctcgtc
      661 taaagaatca cattgattgt ggtgcgggat aggtttgatc gctcctgtcc gacaagcgac
      721 ttgcgcgacc cgcgtatcgg atcaatcatg acatctggaa ggagcgggat cgctccagcg
      781 gtctgctccg cgtgtggaaa aatccgcacg tgataggagg agatgcgcat gacaagatcc
      841 gcatggaagg cggagcctgc gggcccacgc tccgtcgcgc ttgtaggccc ttacggcacc
      901 ggcaaatcca ttttgtttga ggcactgctg gcaagtgccg gcgcggcgcc gaagcggccc
      961 tcagcccgcg cgatgaccac cgagctgcgg ctcgggcatt gcacgttcat gggtgaaccc
     1021 tgggcgctca tcgattgccc tggctcggtc gaattcgcgc aggagacggc ggcggcgctc
     1081 gctgtcgtcg attttgcgat cgtcgtctgc gaaccttccg ccgaccggct cgccaatctt
     1141 ccttttctct tgaagacgat tgaggcggcg ggtctgccgc atcttatctt catcaataaa
     1201 atcgatgcgc ttaccggcca ggtccgcgag atcgtcgcgg cgctgcagcc ccattcccaa
     1261 tatccacttg tcttgcgaca aattccgatc cgcaaacatg acgccgtgac cggctatatc
     1321 gatgtcgtaa gcgagcgtgc ctatcattat tgccgcgaag attccgaacg ttgcatgttg
     1381 ccgagcgaat tgctgatacc cgagcaggaa gcgctcgccg ggctcacgga ggtgctcgcc
     1441 gatcacgacg atgctttgct cgaaaaggtg atcgaggata tcgcgccgtc gcccgacgag
     1501 attttcgcga cgctgcataa ggatcaggaa agcggaacga tcgtccaagt gctcttcggc
     1561 gccgcggcgc gcgggcatgg cattttccgg ctctggaagg ctctgcgcca cgacgtgccg
     1621 gcggcttcgc agaccgcaca acgacgcgcc attgccccgt ccgatcaacc gctcgtgcaa
     1681 atcttcaaga cggttcatgc cgggcaggcc gcctatgcgg gcaaactctc ctatgcgcgc
     1741 atctggcgcg gcggtttacg cgacggcgcg ccgctcaacg gcggccgtgt cggcggcctc
     1801 tatcgcttcg tcaacggcga gattgcgcgc acgcaaacgg cagatccggg cgaactggtc
     1861 gccattgggc ggctcgaagg cgcgatgacc ggcgccgtta ttggcgaagg cagcgcggag
     1921 cttccttttc cgcacccgcc tgtggcggtt tacgccttcg ccatcgccgc caaggaccgc
     1981 aaagatgatg tgaaactgtc cggggcgctg cataaaatcg tcgaggagga cccgtcactc
     2041 agcatcgcgc atcggcgcga gaccggcgag accctgcttt gcggccaagg cgaaattcat
     2101 ctgaacggca cgctggagcg tctggcctcc gtctacaatt gccagatcga tgtcgcgccg
     2161 gcgaaggttg cctataagga gacgatcaag cgcggggtga aacagcatgc acggctgaaa
     2221 cgccagaccg gtggccacgg gcaatttgcc gatgtggtgc ttgagattgc gccgcgcgcg
     2281 cggggcgaag gtttttcctt cgtcgacaag atcgtcggcg gcgccgtgcc gcgccaatat
     2341 attccctcgg tcggcgaagc ggcggaagaa gcgatgcata agggtccgct cggctatcct
     2401 gttgtcgacg tcgaagtgat cctcctcgct gggtcgtttc atgcggtcga t
//
LOCUS       DASWOG010000237         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_41867,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000237 DASWOG010000000
VERSION     DASWOG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41867"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VGB93_04855"
     CDS             <1..751
                     /locus_tag="VGB93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0145474.1"
                     /translation="GRSIVGNARTLLSPVTLELGGDDAAILLPDGSIEAMTTAATLTG
                     LANCGQFCGAVKRIYAPRNLYEAVCESLTERAKKIVIGDGLSPGVEMGPIQNRAQFDK
                     VCAIVDDARAAGGRILTGGEPMAGDGYFYPPTLVADLTDGVRLVDEEQFGPVIPIVAY
                     DDLNHVIDNLNAGPYGLTSSIWTADLDRGAELASRLAVGTGLINQHGGFDASVPMSLI
                     KQSGIGVDYAEYGVKGAMDLQVINVNKTVRH"
     gene            1478..2530
                     /locus_tag="VGB93_04860"
     CDS             1478..2530
                     /locus_tag="VGB93_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEY0145475.1"
                     /translation="MIKFLSSPLRNLAAGLAFMLVVSACATAAYVANGWRLGDALYMV
                     VLTVYTVGYDEVVPINTPELRAITIALIVTGCTGMIFLTGTLIQLITASQLQQLFGFR
                     RMQKEIDRLTDHVIICGYGRIGQMLARELQAGKSDFVIVERGDGRMTAARELGFLSLQ
                     GDATDEDVLRLAGISRARALATVLPDDAANVFITLSARSLNRELTIIARGEAASTEGK
                     LIQAGADRVVLPTRIGAERMAELLLYQNVAKVLSGIKAGDLDRLGRDLRRLGLEIEVV
                     AAETGSQSVGATIGELEATAAGAFLVVAIERQNGEILLQPAANVAIQEGDGVAIVGRP
                     GRAQTVVSVFTRSTVS"
     gene            2589..3002
                     /locus_tag="VGB93_04865"
     CDS             2589..3002
                     /locus_tag="VGB93_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY0145476.1"
                     /translation="MKSSATTEGEHPSGLIDAKIKELGDWRGAMLAHVRALIRQAHPD
                     IVEEWKWRGVPVWSHDGIICTGEAYKSIVKLTFAKGASLEDLSSLFNASLEGNTRRAI
                     DLHEGDKVDADAFKELIRAAVALNESSARLKKRKP"
     gene            complement(3009..>3264)
                     /locus_tag="VGB93_04870"
     CDS             complement(3009..>3264)
                     /locus_tag="VGB93_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145477.1"
                     /translation="VAASALTRGLAAIQFPPEACCIQSGALYVYDRAALAALLQVRAV
                     AAAAAGLPVRVDAFAAHIASTWFERGHPAHPIIAAAFGES"
BASE COUNT          689 a          974 c          977 g          624 t
ORIGIN      
        1 gggccgctcg atcgtaggca atgccagaac gcttctgagc cccgttacgc tggaactcgg
       61 cggggacgac gcagcaatcc ttctgcccga cggatcgatc gaggccatga cgacggccgc
      121 gaccctgacc ggccttgcga attgcggcca gttctgcggc gctgttaaac gcatctatgc
      181 gccccgcaac ctttacgaag cggtctgcga gtctttgacg gagcgggcca aaaaaattgt
      241 tatcggcgac ggcctgtcac ccggcgtcga gatgggtccc atccaaaaca gggctcagtt
      301 cgacaaggtt tgcgccatcg tggatgacgc tcgtgctgct ggcggccgta ttcttactgg
      361 cggcgaaccc atggctggcg acggatattt ctacccgccc acattggttg cggacctcac
      421 cgacggcgtt cgactggtcg acgaggaaca gtttgggccg gtcattccca tcgtggccta
      481 tgacgacctg aaccacgtga tcgataatct caatgccggc ccttatggtc tcacaagttc
      541 gatctggacc gccgacctcg atcgcggggc ggagctggca tcacgcctgg ctgtcggtac
      601 gggtctgatc aatcaacatg gcggattcga cgcgtccgtt cccatgtcgc tcatcaaaca
      661 atcgggtatc ggcgtggatt atgcggagta cggcgtcaaa ggcgccatgg accttcaggt
      721 gatcaacgtc aacaagactg tgaggcacta gcgaagagag tcgcgtcgag cgataggcgc
      781 gggaacttat ggttaatgac accaatcaag ctaccgctta gtgcgtcatg tccaacgccg
      841 gttcgttcat acgccaagcg cttcgagcag cgcggtgcgt agtttttcaa atctcggacc
      901 aaagtgacga cgggccattc aagcggaatg tgcaattcgg ttcggaggcg tcccgcctcc
      961 aggacgacgg ctcagtcagc aggcagcaac gcctcatcca cgtcatgcgc taggagaaga
     1021 accgaacaat gatgcttggc ccaaagctcc gaaacaagcc gctgcgcacg atttgatcag
     1081 agttcaaacc gaggaggcca taaaccagga gggaggcggc cacgatattc cttcccttag
     1141 gacgcctccg gagacgtcag acgctcccac aagcttccat cttcccaaag aggccagatg
     1201 cttctttggc cgccctcggg actgcgcaag caatcgtggc cgacgcccac cccgtaaagg
     1261 ttgccggcag acggcgcgca gcattcgacg taagtgccag ctcgcagccg cacctttaga
     1321 aaattccgat tgaatttcgg ccccgattca attccccttg aaactgttcc accctgattg
     1381 gtgggaccaa atattgcttc caaaagattt cctgtggctt ttcccgccgg acgtctgggt
     1441 cgccgccgcc gcgtcgacac gctcagattt ctgattgatg attaagttcc tatcctctcc
     1501 acttcgaaat ctggctgccg gcctcgcgtt catgctcgtg gtctcggcct gcgcgaccgc
     1561 ggcgtatgtg gcgaacgggt ggaggctcgg cgacgcgctc tatatggtcg tgctcaccgt
     1621 ttacacggtc ggctatgacg aagttgtacc gatcaacacg ccggaattgc gcgccattac
     1681 catcgcattg attgtcacgg gctgcaccgg gatgatcttt ctgaccggca cgctcattca
     1741 gctcatcacc gccagccaac tccaacaatt atttgggttc cgccggatgc aaaaagaaat
     1801 cgaccgcctc accgatcacg tcatcatctg tggttacggc cgcatcggcc agatgctcgc
     1861 gcgagagctt caggcaggaa agtccgactt tgtcatcgtc gagcgcggcg acggccgcat
     1921 gaccgccgcg cgcgaactgg gcttcctcag cttgcagggc gacgccacgg atgaagacgt
     1981 cctgcggctt gccggtatct cccgggcgcg ggccttggcg accgtcttgc cagatgacgc
     2041 ggcgaatgtg ttcatcacgc tcagcgcccg cagtcttaac cgcgaactga cgatcattgc
     2101 gcggggcgaa gccgcatcca cggaaggcaa gctcattcaa gccggtgccg atcgtgtcgt
     2161 gctgccgacg cggatcggcg cagaacggat ggctgagctt ttattgtacc agaacgtcgc
     2221 caaggtcttg tcgggaatta aagccggcga cctcgaccgg ctcggccgcg atctgcgccg
     2281 tctaggtctt gaaatcgagg ttgtcgccgc ggaaaccggc agccagagtg tcggggctac
     2341 cattggcgag cttgaggcca ctgccgcagg cgcgttcctc gtcgtcgcga tcgagcggca
     2401 gaatggggaa atcttgctcc agccggcggc aaatgttgca atccaggaag gcgacggcgt
     2461 tgcgatcgtc ggcaggccag gccgagccca aaccgtcgtg tccgttttca cgcggtcgac
     2521 cgtgagctga atcttcggct acgccgccgg cgccaaattc acggacgtat ttgggaggcc
     2581 gcagcaagat gaaaagcagc gcaacgaccg agggcgaaca tccttccggg ctgatcgatg
     2641 caaagatcaa ggaattaggc gattggagag gcgcgatgct cgcccatgtt cgcgcgctca
     2701 tcagacaggc gcaccccgat attgtcgaag agtggaaatg gcggggcgtt cctgtgtggt
     2761 ctcatgacgg gatcatctgc accggggaag cctacaagag catcgtgaag ctgaccttcg
     2821 ccaaaggcgc ttcgctcgaa gacctctcaa gcctgttcaa cgccagcctc gaaggcaaca
     2881 caaggcgcgc gatagatctg catgaaggcg ataaagtcga tgcggatgcc ttcaaagaac
     2941 tcatccgcgc cgccgtggcc ttgaacgagt cgtcggcgcg attgaaaaag cggaagccgt
     3001 aggcagactc agctctcgcc aaacgctgcc gcgatgatcg gatgcgcggg atggccgcgt
     3061 tcaaaccagg tggaggcaat gtgagctgca aaagcatcga ctcggacagg aagtccagcg
     3121 gcggccgccg cgacggcacg tacctgtaga agagcggcaa gcgccgcgcg gtcatagaca
     3181 taaagtgcac cgctctggat gcagcacgct tcaggcggga attgtatagc agccaatcca
     3241 cgtgtcaggg cggaggcggc gaca
//
LOCUS       DASWOG010000238         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_60460,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000238 DASWOG010000000
VERSION     DASWOG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60460"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..814
                     /gene="fabI"
                     /locus_tag="VGB93_04875"
     CDS             14..814
                     /gene="fabI"
                     /locus_tag="VGB93_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0145478.1"
                     /translation="MAGKRGLVLGAANNRSIAWGIAKAAHAAGAELAFTYQGESLEKR
                     VRPLAEELQAAVVGHCDVTDEASIDAVFGEIERLWGSLDFVVHCVAFSDKDQLTGRYV
                     ETTADNFTNTLLISCYSFTAIAQRAEKLMTNGGSLLTLTYYGAEKWMPHYNVMGVAKA
                     ALEASVRYLAADLGQKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRSVTI
                     DEVGESAAFLLSPMSRGITGEIMHVDAGYHIVGMKNPAAPDMSALIKE"
     gene            869..1957
                     /gene="aroC"
                     /locus_tag="VGB93_04880"
     CDS             869..1957
                     /gene="aroC"
                     /locus_tag="VGB93_04880"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0145479.1"
                     /translation="MSHNTFGHLFRVTTFGESHGPAIGCVVDGCPPGIALTEADIQHY
                     LDERRPGTSRFTTQRQESDKVRILSGVFEDRHTGAQVTTGTPIGLLIENEDQRSKDYE
                     AIKDKFRPGHADYTYEAKYGIRDYRGGGRSSARETAMRVAAGAIARKVLGNVIVRGAL
                     VAMGSEWIDRAKWDWEETRRNAFFSPDADKAREFEAQLDAIRKSGSSIGAVIEIVAEN
                     VPAGWGAPIYGKLDSELASALMSINAVKGVEIGDGFAAAALSGEENADEMRMSNDGLP
                     IFSSNHAGGILGGISTGQAIVVRFAVKPTSSILAPRKTIDLSGAETEIVTKGRHDPCV
                     GIRAVPVGEAMVACVLADQFLRHRAQVG"
     gene            complement(1954..>2179)
                     /locus_tag="VGB93_04885"
     CDS             complement(1954..>2179)
                     /locus_tag="VGB93_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY0145480.1"
                     /translation="NGVGVHDGHIVVFAISDDPVTFYQFATLFRDRLNCPNALFLDGS
                     VSSLYAPAVGRDDALLPLGPIVGVINNGLR"
BASE COUNT          441 a          641 c          678 g          419 t
ORIGIN      
        1 cgcccccgga attttggccg gtaaacgcgg cctcgtgctg ggcgccgcca ataatcgttc
       61 gattgcctgg ggcattgcca aggcggccca tgcggcgggc gccgagcttg ccttcaccta
      121 tcaaggcgag tcgcttgaga agcgcgtgcg tccgcttgcg gaagaattgc aggcggccgt
      181 tgtcggccat tgcgacgtga ccgacgaagc gtccatcgac gcggttttcg gcgagatcga
      241 acggctctgg ggcagtctcg atttcgtcgt ccattgcgtg gcgttttccg acaaggatca
      301 actcacgggc cgctacgtcg agacgacggc agacaatttc accaacacac tgctgatctc
      361 ctgctattcc ttcactgcga tcgctcagcg tgccgaaaag ctgatgacga acggcggctc
      421 gctgctgacg ctcacttatt acggcgccga gaaatggatg ccgcattata acgtgatggg
      481 cgtcgctaag gcggcccttg aagcctccgt gcgctatctc gccgccgatc tcggccagaa
      541 aaacatccgc gtcaatgcga tttcggccgg cccgatcaag acgcttgccg cgtccggcat
      601 cggcgacttc cgctacatcc tcaagtggaa cgaatataat gcgccgctgc gccgctcggt
      661 gacgatcgac gaagtcggcg agagcgctgc gtttctactc tcgcccatgt cgcgtggcat
      721 caccggcgag atcatgcatg tcgatgccgg ctatcatatc gtcggcatga aaaatcctgc
      781 cgcgccggat atgtcggccc ttatcaagga atagacgctt gatcacgaaa agtttcagac
      841 ttttcggata acatcatgcg cgaaaacaat gtcgcataat acgttcggcc acctgtttcg
      901 cgtcacgacc tttggcgaga gccacgggcc ggcgatcggc tgcgtggttg atggctgtcc
      961 gcccggcatc gcgctcaccg aggctgacat tcaacattat ctcgacgagc gtcggccggg
     1021 cacgtcgcgg ttcacgacac agcggcagga gagcgataaa gtccgcatcc tctccggcgt
     1081 gtttgaagat cgccacacgg gcgcacaagt gacgaccgga acgccgatcg gtttattgat
     1141 cgagaatgag gatcagcgct cgaaggatta cgaggcgatc aaggacaagt tccgtccggg
     1201 tcatgccgat tacacctacg aagcgaaata cggcattcgc gattatcgcg gcggcggccg
     1261 gtcctcggcg cgcgagacgg cgatgcgcgt cgcggccggc gccatcgcac gaaaagtcct
     1321 tggcaatgtg atcgtccgcg gcgcgctcgt tgcgatgggc agcgaatgga tcgatcgcgc
     1381 caaatgggat tgggaagaga cgcgccgcaa tgccttcttt tctccggatg ccgacaaggc
     1441 gcgggagttt gaggcgcagc tcgatgccat ccgtaagtct ggctcgtcga tcggcgcggt
     1501 gatcgagatc gttgccgaga acgtgccggc gggctggggt gcgccgatct atggcaagct
     1561 cgattcggaa ctcgcttcag cgctgatgtc gatcaacgcg gtcaaaggcg tcgagattgg
     1621 cgatggcttt gccgcagcgg cgctatcggg cgaagaaaac gcggacgaga tgcgcatgag
     1681 caatgacggc ctgccgatct tttcgagtaa tcatgccggc ggcatcctcg gcggcatttc
     1741 gacagggcag gcgatcgtcg tgcgcttcgc ggtgaagcca acctcttcaa tcctggcgcc
     1801 acgtaaaacg atcgatcttt ccggcgcgga aacagagatc gttaccaaag ggcgacacga
     1861 tccctgcgtc ggcatccgcg cggttccggt cggtgaggct atggtcgcct gcgtgctggc
     1921 cgaccagttc ctccgtcatc gcgcacaggt cggttaacgc aagccgttgt tgatgacgcc
     1981 gacgattggg ccgagcggca acaaggcatc atcgcggccg acagccggcg cgtagagact
     2041 cgaaactgag ccgtcgagaa aaagcgcgtt cggacaattc agccggtcgc gaaacagcgt
     2101 ggcgaattga tagaacgtca ccggatcgtc agagatcgcg aagacgacga tatggccgtc
     2161 atgcactccg acgccgttg
//
LOCUS       DASWOG010000239         4547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_262435,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000239 DASWOG010000000
VERSION     DASWOG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262435"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1677
                     /locus_tag="VGB93_04890"
     CDS             142..1677
                     /locus_tag="VGB93_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0145481.1"
                     /translation="MSEADRLAVASGVPSSTLMERAGAAVAKAAGRLIGGRSAQGARV
                     AVFCGPGNNGADGFVAARHLRAEGFNISVGLLGTPSQLKGDAARAAEAWGETILPFDK
                     VPLDEANLVVSAIFGAGLSRDVDGEARAVILRLNDWTRRTRKPILSIDIPSGIDGSTG
                     LVRGVAIEATQTITFFRLKPGHLLLPGQIHCGEISLVDIGIPESVLATIKPQTFANGP
                     ALWGAHYPQPHVDGHKYKRGHALVLSGSLAHTGAARLSARGALRAGAGLVTVATPTDA
                     LAVHAAALTAIMTRVCDSAADLTELLGDKRKNAIVMGPGLGVGEATREFVLTALAQPD
                     APGEPRAVVLDADALTSFAGDAMGLASAIKASGKIVVLTPHDGEFAQLFATKEGELRD
                     HPRVELLASPSKLDRTRAGAALMGAILLLKGSDTVVAEPNGRASINHGAPPWLATAGS
                     GDVLSGIIAGLLAQAMPAFEAASAGTWLHAAAAREFGPGLIAEDIPEYLPPVLRRLLE
                     NDL"
     gene            complement(1674..3005)
                     /locus_tag="VGB93_04895"
     CDS             complement(1674..3005)
                     /locus_tag="VGB93_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0145482.1"
                     /translation="MILSRRVLLGSTLATLIAHHHERRASPEPSVPKAPPRRLIAQKA
                     QVRLMPAAAPETGILGFDGTAPGPLLRCKLGDELAIRLVNQTDQPLTLANWGLRIPNA
                     LDGVAPLTQEPVPPGGSFDYTFKPVDAGLFGYRSFVAPDAGNQLRHGLYGALIVDEAD
                     PPPADKDIVAILSDWQLDAKTQILASAQAGMSLATLNSRPLATEETHKPGSRIRLRLL
                     NAAAARLFFVAFENVSPHVLAIDGQPCAAFSPGGNMLPIGPGGRFDVMFDLPDKPGTA
                     RLLLRDDATHDRPLMVFRTEGNKWPDIDPIPNLPPNPLLPAKIRLEAAKNLQILISGG
                     GAAPFGLNSAAAKDFDKASLFSIKRGVPATLALVNKSSAAQQMRVHGHALRILHDLDD
                     GWDPYWRDSVIAPPGHTKHIAFVADNPGKWAIEMLPLDVPAGDMVTWFEVL"
     gene            3148..3232
                     /locus_tag="VGB93_04900"
     tRNA            3148..3232
                     /locus_tag="VGB93_04900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3182..3184,aa:Leu,seq:tag)
     gene            3331..>4547
                     /gene="tig"
                     /locus_tag="VGB93_04905"
     CDS             3331..>4547
                     /gene="tig"
                     /locus_tag="VGB93_04905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0145483.1"
                     /translation="MQVTETRSEGLKREYKVVLPAGDLASRVETQLNEIKAKAQIKGF
                     RPGKVPVTHLRRLYGRSIMAEVMQEAVNDANRKIIEDNSLRLAGAPRIDLPKDQDELE
                     KAFESKADLSYIVALEVLPKVEVGSFDDVQIERLVADIPDADVDAAIKTIAERNRPFT
                     PKEGEAIAENGDKVTIDFVGKVGGEEFQGGSAQDIDIVLGAGSFIPGFEEQVVGLKVG
                     EQRTINVTFPETYSEATLAGKPAEFDVTLKAVAAPGTLDINDEFAKGLGFDDIEKLRQ
                     QMRGSLEREASVVSRRKWKRELLDALDRKYSFELPQELVDREFDAIWQQAEAELKASG
                     RTLADEGTTEEQERGAYRRIAERRVRLGLALAEIGDSAGVKVSDEEVAQALYERARQY
                     PGMEKQFVDFYRN"
BASE COUNT          838 a         1341 c         1509 g          859 t
ORIGIN      
        1 gacgcccccg cagaagcggc ataaatatgt tgctacgcca cggcgatgtc tcgattttct
       61 gcgagtttgc ggtgggctgc acaaggctgg gtgatcatgt tgtcgcgttt tttcccccct
      121 gaattattga cgacgcgcga aatgagcgag gcggaccgcc tcgccgtcgc gtcaggcgtg
      181 ccgagttcga cattgatgga gcgcgccggc gcggctgttg ccaaggccgc gggacggttg
      241 atcggcggcc ggtccgcgca aggggcgcgc gtcgccgtct tctgcggccc gggcaacaat
      301 ggcgcggatg ggtttgttgc cgcgcggcac ctgcgcgcgg aaggattcaa tatttccgtt
      361 ggtctgctcg gtacgccatc gcagctcaag ggcgatgccg cgcgggcggc cgaagcctgg
      421 ggcgaaacta ttctgccgtt cgataaagtt ccgttggacg aagcgaacct ggtcgtttcg
      481 gcaatcttcg gcgctggcct ttcgcgtgac gttgacggcg aagcacgcgc cgtgatcctg
      541 cggcttaacg attggacgcg gcgcacgcgc aagccgatcc tctccatcga tattccctcg
      601 gggatcgatg ggtcgacagg actggtgcgt ggcgtagcca tcgaggcgac gcagacaatc
      661 acgttttttc gattgaagcc cggacatctt ttacttccgg ggcagataca ttgcggcgag
      721 atcagcctcg tcgatatcgg catccccgag tccgtactcg cgacaatcaa gccgcagaca
      781 tttgccaacg gtccggcgct ctggggtgcg cattatccgc aaccgcatgt cgacggacat
      841 aagtacaagc ggggccatgc gctcgtcttg tccgggtcgc tcgcgcatac cggcgcggcg
      901 cgcttgtccg cgcggggtgc attacgtgcc ggtgccggtt tggtaactgt tgcgactccg
      961 accgatgcgc tggcggttca tgcagcagcc ttgaccgcga ttatgacccg cgtttgcgac
     1021 agcgcggcgg atttaaccga gcttcttggt gacaagcgca agaacgcgat cgtcatgggc
     1081 ccgggtctcg gtgttggcga agcgacgcgc gaatttgtgc tgaccgcttt ggcgcaaccc
     1141 gatgcgccgg gcgagccgcg cgccgtcgtt ctcgacgccg atgctttgac gagtttcgcc
     1201 ggtgacgcga tgggacttgc gagcgcgatc aaggcttcgg gcaaaatcgt cgtgctcacg
     1261 ccgcacgatg gcgaattcgc gcaattgttc gccaccaaag agggcgaatt gcgcgatcat
     1321 ccgcgggtcg aattgctcgc gtcgccctcc aagctcgatc ggacccgcgc cggcgctgcc
     1381 ttgatgggcg cgatcctttt gctgaagggg tcggatacgg tggtggcgga gcccaatggg
     1441 cgcgcctcga tcaatcatgg cgcgccgccg tggctcgcga ccgcgggctc tggcgatgtg
     1501 ctctctggta tcatcgcggg gctgttggcg caggcgatgc ccgcgttcga ggctgcgagc
     1561 gccggaacgt ggcttcatgc cgccgcagcg cgcgaattcg gtcccggtct catagccgaa
     1621 gatatcccgg aatatctgcc gccggtgttg cggcgcctgc tcgaaaatga tctttagagc
     1681 acctcaaacc acgtcaccat gtcgcccgca ggcacgtcga gcggcagcat ttcgatggcc
     1741 catttgcccg gattatctgc gacaaaggcg atgtgtttgg tgtggcctgg cggcgcgatg
     1801 acgctgtcgc gccaataggg gtcccagccg tcgtcaaggt cgtgcaggat acgcagcgcg
     1861 tgtccgtgaa cgcgcatctg ctgcgcggcg ctggatttat tgacgagtgc aagcgtcgcc
     1921 ggcacgccgc gcttgataga aaacagcgac gctttgtcga aatcctttgc tgccgcgctg
     1981 ttgagaccga acggagccgc gcctccgccg gagatgagga tttgaaggtt cttggccgcc
     2041 tccagccgaa tctttgccgg caaaagcgga tttggcggca aattcgggat cggatctatg
     2101 tccggccatt tgttgccctc cgtccggaag accataagcg gccggtcgtg cgtggcgtcg
     2161 tcgcgcaaaa gcaggcgcgc cgtgccgggc ttgtccggca gatcgaacat cacgtcgaac
     2221 cgtccgcctg ggccgatggg cagcatgttg ccgcccggcg aaaaggccgc gcatggctgg
     2281 ccgtcgatcg cgagcacatg cgggcttaca ttttcgaaag cgacaaagaa gagccgcgcg
     2341 gcggccgcat tcaacagccg caaacggata cggctgccgg gcttgtgagt ctcttccgtc
     2401 gcaagcggcc tcgaattaag agttgcgagc gacatcccgg cttgcgcgga agcaaggatt
     2461 tgggttttgg cgtcaagctg ccagtcgctc agaatggcca cgatatcttt gtcggccggt
     2521 ggaggatcgg cttcatccac gatcagcgcg ccgtagagtc cgtgccggag ctggttcccg
     2581 gcgtccgggg cgacgaagga gcgatagccg aagaggccgg catccaccgg tttgaaggta
     2641 tagtcgaagc tcccgcctgg cgggacgggc tcttgcgtca gcggcgcaac cccgtcgaga
     2701 gcatttggaa tgcgcaaccc ccaattcgcc agggtcagtg gctggtccgt ctgattgacc
     2761 agccgaatcg caagctcgtc gccgagcttg caccgcaata aagggccggg ggcggtgccg
     2821 tcgaaaccga gaatgccggt ttcgggcgcg gctgccggca tcaagcggac ttgtgccttc
     2881 tgggcgatga gccgccgcgg gggggccttg ggcacggagg gctctggcga cgcgcgtcgc
     2941 tcatgatgat gggcgatcaa ggttgcgagc gtcgagccga gcagcacgcg gcgggaaagg
     3001 atcatgcgag ggttccagtc atgccctttt gctagccatg attccggtaa aacgataggc
     3061 ggcggaaacg tcactttttt gctgcggccg ccatcgggcg tgctatagac gcggcccgag
     3121 cggtaaacgc cgcgctctgc ggcgcgcgcg ggcgtggcgg aactggtaga cgcgccggat
     3181 ttaggttccg gtggtgaaaa ccgtgggggt tcgagtccct ccgcccgcac caatcgcata
     3241 atttcgcgcc ctgtccgaag cttaacggcg ccggccgcgg cttttgtttt gaaggcagcg
     3301 gtcgaaaatt taaagaaggt ggatgagccg atgcaagtga cggaaacacg ctccgagggc
     3361 ctgaagcgcg aatataaggt cgtgctgcca gcgggcgatc tcgcctcgcg ggtcgagact
     3421 cagctcaacg agatcaaggc gaaggcgcag atcaagggtt tccgccccgg caaggtgccg
     3481 gtcacgcatt tgcgccgcct ctatggccgc tcgatcatgg ccgaggtgat gcaggaggcg
     3541 gtcaacgacg ccaatcgcaa gatcatcgaa gacaattcgc tacgcctcgc cggtgcgcct
     3601 cgaatcgatc tgccgaagga tcaggacgaa cttgaaaagg ccttcgaatc caaggccgat
     3661 ctctcctata ttgtggcgct tgaagttctg ccaaaagttg aagtcggcag cttcgatgac
     3721 gtccagatcg aacgtctcgt cgccgatatt cccgatgccg acgtcgatgc cgcgatcaag
     3781 accatcgccg agcgcaaccg cccgttcacg cccaaagaag gcgaggcaat tgccgagaac
     3841 ggcgacaagg tgacgatcga tttcgtcggc aaggtcggcg gcgaggaatt ccagggcggc
     3901 agcgcgcagg acattgatat cgtccttggc gccggctcgt tcattccggg cttcgaagag
     3961 caggtcgtcg gcctgaaggt cggcgagcag cgcacaatca atgtcacctt ccccgagacc
     4021 tattcggaag ccacgctcgc cggcaagccg gccgaattcg acgtgacgct gaaggccgtc
     4081 gcggcgccgg gtacgctcga catcaacgat gagttcgcca agggcctcgg tttcgatgat
     4141 atcgagaagc tgcgtcagca gatgcgcggc tcgctcgagc gtgaagcgag cgtcgtctcg
     4201 cgccgcaagt ggaagcgcga actgcttgac gcgctcgaca ggaaatattc cttcgaactg
     4261 ccgcaggaac tggtcgaccg cgagttcgac gcgatctggc agcaggccga ggccgagttg
     4321 aaagcctcgg ggcgcacgct tgccgatgaa ggcacgaccg aggagcagga acggggcgcc
     4381 tatcgccgga tcgccgagcg ccgcgtgcgt cttggcttgg ccttggccga aatcggcgac
     4441 agcgccggtg ttaaagtttc ggatgaggaa gtcgctcagg cactttatga gcgtgcgcgc
     4501 caatatcccg gaatggaaaa gcaattcgtc gatttctacc gcaacaa
//
LOCUS       DASWOG010000240         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_170559,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000240 DASWOG010000000
VERSION     DASWOG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170559"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VGB93_04910"
     CDS             <1..1336
                     /locus_tag="VGB93_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0145484.1"
                     /translation="SALSHSAAPSLWLALPFAGLLLSIALGPILARRIWHVHYGKAAA
                     FWAALALVSLLATQGLMPTLAPVAHTLLADYLPFILMLFALYTTAGGIVVSDLAPATP
                     RVNTLLLATGTMAASLIGTTGAAMILIRPVLQANAARQHKVHVVIFFIFLVANIGGAL
                     SPLGDPPLFFGFLRGVNFFWPARHLWPQFALVAALLLAIFFALDSYFFAKEPKIAPVQ
                     TWLEVRGLKNVALIAAAVAAIIGSAIWRPRINFEILGTHLALQDLVRSVALLAIGLLS
                     IALTPRADHEANHFSWEPLEEVAKLFAAIFICIIPVMAMLAAKTQGPFAPVIALLSHP
                     NGAPHDAAYFWATGLLSSMLDNAPTYLVFFGLAGGNATALSGELARTLAAISLGAVFM
                     GALTYIGNAPNFMVYALARRARVPMPSFFGYLAWSGVILVPVFVLVTLVFFM"
     gene            complement(1340..1993)
                     /locus_tag="VGB93_04915"
     CDS             complement(1340..1993)
                     /locus_tag="VGB93_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY0145485.1"
                     /translation="MTETAEKPLAAKPSPWLKITLELGPLLLFFFANSRPKLFAPYAH
                     LVLPAHLLVGENAGLFTATVVLIPAVLIALVVSYVQLRRLPVMSLVTAILVVIFGALT
                     LYFQDPRFIKMKPTFLYAIFGLALLGGLYLKKPILEIVLDNAMPLTEEGWRILTLRWG
                     VFFLGLAILNEIIWRTQSNNVWVAFKFPGVVIIIFLFTIAQVPLMMKHELKDANTAD"
     gene            complement(2004..3137)
                     /gene="ftsY"
                     /locus_tag="VGB93_04920"
     CDS             complement(2004..3137)
                     /gene="ftsY"
                     /locus_tag="VGB93_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0145486.1"
                     /translation="MADADPTGAKPKRSLFGRLLGRTENESAPTPDALLPEAPQPAES
                     VVPDASAPEPVSETPPITPDAPEPPPKLSWWKRLREGLSRSSQAIGGGINDIFSKRKL
                     DADTLQELEDVLIRADLGATASLRITEAVAKGRYDKNVSPDEVKNILATEIESVLEPL
                     AQPLEIDRSKRPFVILVIGVNGSGKTTTIGKLAAKFSRDDGLKVLLAAGDTFRAAAIE
                     QLRIWGARLNCEVIARESGGDAAGLGFDALKAAREQNADLLIMDTAGRLQNRTELMAE
                     LEKIIRVMKKFDPEAPHAVLLVLDATVGQNALNQVEIFSRVAGVTGLVMTKLDGTARG
                     GILVAIAENFKLPIHFIGVGESADDLESFTARDFARAIAGLDE"
     gene            complement(3259..>3674)
                     /locus_tag="VGB93_04925"
     CDS             complement(3259..>3674)
                     /locus_tag="VGB93_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145487.1"
                     /translation="GYVFVAKALPLCKAPGVRAMVPLSKLIGAASLFLAMTCAFAAPS
                     LAASPYELTVVPPVPSGSPFIYRINVASGEVSYVSGNNFLALKDARPIPPGSYHLHLQ
                     MSSDGKGTYWLYRMDTETGRTWFLGAGAWTEIVLK"
BASE COUNT          668 a         1083 c         1130 g          793 t
ORIGIN      
        1 aagcgcattg agccattccg cggcgccgtc cttatggctc gcgctgccgt tcgcggggct
       61 tttgctttcc attgcgctgg ggccgatcct cgcgcggcgg atatggcatg tgcattacgg
      121 caaggcagcc gccttctggg cggcgctggc gcttgtctcg ctgcttgcca cgcagggttt
      181 gatgccgacg cttgcaccgg tcgctcacac tctgctcgcg gattatctgc ccttcatcct
      241 gatgctgttc gcgctttata cgaccgcagg cggcattgtc gtctccgatc ttgcgccggc
      301 gacaccacgc gtcaatacgc tgcttctggc gaccggcaca atggcggcga gtcttatcgg
      361 cacgacaggt gccgcgatga tcctgatccg gccggtgctg caggccaatg ccgcccggca
      421 gcacaaagtc catgtcgtca ttttcttcat ttttcttgtc gccaacatcg gcggcgcgtt
      481 gagcccgctt ggtgatccgc ccctattctt cggctttctg cggggtgtca atttcttctg
      541 gccggcgcgt catttgtggc cgcagttcgc gctggtggcg gccctgctac ttgcaatctt
      601 cttcgcgctc gactcctatt tcttcgcgaa ggaaccaaaa attgcgccgg tgcaaacctg
      661 gcttgaagtt cgcgggctga agaatgtcgc gctgatcgcc gccgcggtcg ccgcgatcat
      721 cggtagcgcg atctggcgtc cccgcatcaa tttcgaaatt ttgggtacac acctcgcctt
      781 gcaggatctt gtgcgcagcg tcgccttgct tgccatcggc ttgctttcga ttgcgctgac
      841 accgcgggcc gatcacgaag ccaatcattt ttcctgggag ccgttagaag aggtcgccaa
      901 actctttgca gcgattttca tctgcattat tccggtgatg gcgatgctgg cagcgaagac
      961 gcaggggccg ttcgcgccgg tgatcgcact cctgtcccac ccgaatggtg cgccgcatga
     1021 cgcggcctat ttctgggcga ccgggctgtt gtcgtcgatg ctcgacaacg cgccgaccta
     1081 tctggtcttc ttcgggctcg ccggtggcaa cgcaacggcg ctttcagggg agttggcgcg
     1141 cacgctcgcg gcgatctcgc tcggtgcggt cttcatgggc gcgctgacct acattggcaa
     1201 tgcgccgaat ttcatggtct acgctttggc gcgccgcgcc cgcgtgccga tgccgagttt
     1261 cttcggctat ctcgcttggt cgggcgtgat tctggtgccg gtttttgtct tggtgacgct
     1321 cgtatttttt atgtgacgtt caatccgcgg tgttcgcgtc cttcaactca tgtttcatca
     1381 tcagcggcac ctgcgcgatg gtaaacagga aaatgatgat cacaacgccg gggaatttga
     1441 acgcgaccca gacattgttg gattgcgtgc gccagatgat ctcgttgagg atcgcaagcc
     1501 ccagaaagaa cacgccccag cgcaaggtga gaatacgcca accttcttcc gtcaacggca
     1561 tcgcattgtc gaggacgatt tcgaggatcg gtttttttag ataaaggccg ccgagcaggg
     1621 ccagaccgaa gattgcatag aggaacgtcg gcttcatctt gatgaagcgc gggtcttgaa
     1681 aatagagcgt caacgcgccg aagatcacga cgaggatcgc ggtgacgagc gacatcaccg
     1741 gcaggcggcg caattgcacg taggagacga caagcgcgat cagcacggcc gggatcagca
     1801 ccaccgtcgc ggtgaagagg ccggcatttt cgcccacaag cagatgcgcc ggcaggacga
     1861 gatgcgcgta gggcgcaaag agcttcggcc gtgaattggc gaagaaaaag agcagcagcg
     1921 ggcccaattc gagcgttatt ttcagccagg gcgacggctt ggcagcgagc ggcttttcgg
     1981 cggtttcagt catgtattga tgctcattcg tcgagcccgg cgatggcgcg ggcaaaatcg
     2041 cgtgcagtga aggattcgag atcgtcggca ctttcgccga cgccgatgaa atggatcggc
     2101 aatttgaagt tttccgcgat ggccacgaga atgccccccc gcgcggtccc gtcgagcttg
     2161 gtcatcacga ggccagtgac accggccacg cggctgaaaa tctcgacctg gttcagcgcg
     2221 ttctggccca cggtggcgtc gagcacaagc agcaccgcgt gcggggcctc ggggtcgaat
     2281 ttcttcatca cacggatgat cttttcgagt tcggccatca attcggtgcg gttctgcaat
     2341 cgcccggcgg tatccatgat gagcagatca gcgttttgct cgcgtgcggc cttcaaggcg
     2401 tcgaagccga ggcccgcggc gtcgccaccg ctctctcgcg cgatgacctc gcaattgaga
     2461 cgcgcacccc agatgcgcaa ttgctcgatc gcggcggcgc ggaatgtatc gccggccgca
     2521 agcaacacct tcaaaccgtc atcgcgggaa aatttggccg cgagcttgcc gatggtcgtt
     2581 gttttgcccg agccgttgac accgataacc aggatgacga agggccgctt gcttctgtcg
     2641 atttcgagcg gttgagcgag gggctcgagc acgctctcga tttcagtcgc gagaatattc
     2701 ttcacttcgt cagggctgac gtttttgtcg tagcggcctt tggcgaccgc ctcggtgatc
     2761 cgcagcgatg ccgtcgcgcc gagatcggca cggatcagca catcctcaag ctcttgcagc
     2821 gtgtccgcat cgagctttcg cttcgagaaa atatcattga tgccgccgcc aatggcctgc
     2881 gacgagcgcg acaagccttc gcgcaggcgc ttccaccagg aaagttttgg tggcggttct
     2941 ggcgcgtcgg gagtgattgg tggggtttcg gagacgggct ccggagccga ggcgtccggt
     3001 accaccgatt ccgcaggttg cggtgcctcc ggcagtaacg cgtccggggt cggcgcactc
     3061 tcattttccg tacggccgag cagtcggccg aaaagagagc gtttcggttt cgctccggtg
     3121 gggtctgcat ccgccaatat gcacctgatt tgctcatccg ctcccaccta tatggcggtt
     3181 ggcagggcga tacaaagtgg gaatgcgccg gaataagggc gccgcgaccc gaggcgccct
     3241 ttgctggctg gccggcgctt atttcagcac aatctccgtc caggcgccgg cgccgagaaa
     3301 ccacgtccgg cccgtctccg tatccatccg gtagagccaa taggttccct tgccgtcgga
     3361 actcatctgc aggtggagat ggtaggagcc cggcgggatc ggccgggcgt ccttgagggc
     3421 cagaaaattg tttccggaaa cataggagac ttcgccgctg gccacgttga tccgatagat
     3481 gaatggtgag ccactaggga ctggcggaac cacggtaagt tcataaggcg acgcggcaag
     3541 agatggcgcg gcgaaggcgc atgtcatcgc gagaaaaagc gaagcagctc caatcaattt
     3601 ggacaaaggc accatggcac gaactcccgg cgctttgcac agcggcaaag ctttcgccac
     3661 aaatacgtag cctg
//
LOCUS       DASWOG010000241         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_134142,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000241 DASWOG010000000
VERSION     DASWOG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134142"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VGB93_04930"
     CDS             complement(<1..447)
                     /locus_tag="VGB93_04930"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY0145488.1"
                     /translation="MKDKKKPAKAKSDDRDSSLKLKKSAAKTREIGDAIFVNDFVVDC
                     NVGVYAEEQGVTQKVRFTVEARLSKDVFSVEDEMAEVPSYADIIDIIVALARGGHIKL
                     VETFAERIAAQVLADERIIAVRVALEKLERGPLRGVEIIRPATRAAA"
     gene            complement(444..1178)
                     /locus_tag="VGB93_04935"
     CDS             complement(444..1178)
                     /locus_tag="VGB93_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HEY0145489.1"
                     /translation="MIDGLRLGKPAFLASVTNADEAEAALAGGADIIDCKDPASGALG
                     ALGADTVRDIVARIGGRLAVSATVGDLPSDPDILVKAAAQMAATGVDIVKVGFFGGDD
                     PGAAISALGIADLGPARLVAVLMADREPDFAVVPELAASGFAGVMLDTADKTAGRLTS
                     VLSIARLTKFVGLARDNDLFVGLAGSLKEGDISVLAGLEPDMLGFRGALCPAGRKSAL
                     ERSRVAAIRRALDAAKLEKMRETSVA"
     gene            complement(1212..>1699)
                     /locus_tag="VGB93_04940"
     CDS             complement(1212..>1699)
                     /locus_tag="VGB93_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145490.1"
                     /translation="FIFQTPRLLPWRTVYENIALVLPDGDPRHVKIPAMLERVGLTQA
                     ANAYPERLSLGMQRRAALARGFILEPEILLMDEPFVSLDDPTAQSLRELLMELWRSHP
                     TTVIFVTHDRTEAIQLGTRILRLAPGKASVAQDVTINLTEAQRHDRAAVLAEQNRIFA
                     A"
BASE COUNT          312 a          565 c          509 g          313 t
ORIGIN      
        1 cgcagccgca cgcgtcgcgg gccgaatgat ctcgacgccc cggagcgggc cgcgctccag
       61 cttttcgagc gccacgcgaa cagcaatgat ccgttcgtcc gcgagaactt gcgcagcgat
      121 ccgctcggcg aaagtttcga cgagcttgat gtgtccgcct cgtgccagcg cgacaatgat
      181 gtcgatgatg tcggcgtagg atggaacctc cgccatctcg tcctcgacgc tgaacacgtc
      241 cttcgaaagc cgcgcttcga ccgtgaagcg caccttctgc gtcacgccct gctcttccgc
      301 atagacgccg acgttgcaat cgacgacgaa gtcattgacg aaaatcgcgt caccgatctc
      361 tcgcgtcttc gcggcggatt tcttgagctt cagcgaactg tcgcggtcat cggacttggc
      421 ctttgccggc ttcttcttgt ctttcatgca accgacgtct cccgcatctt ttcgagcttc
      481 gcggcgtcga gcgcgcgccg gatggcggct acgcgactac gttccaacgc gctcttgcgc
      541 cccgctgggc aaagcgcgcc gcgaaatccc aacatatccg gttcgagccc cgcgaggacg
      601 gagatgtcgc cctccttcaa cgatccggca agaccgacga agaggtcatt gtcgcgggcc
      661 aaacctacga atttcgttaa ccgcgcgatg gaaagcaccg atgtcaaccg gcccgcggtt
      721 ttgtccgccg tgtcgagcat gacacccgcg aagccggacg cggcgagttc cggcacgaca
      781 gcgaaatccg gttcccggtc ggccatcaga accgcgacga gccgcgccgg acccaaatcc
      841 gcgatgccca gcgccgagat cgcggcacca gggtcgtcgc ctccgaaaaa gccgaccttg
      901 acgatgtcga cgccggttgc agccatttgc gctgcggctt tcaccagaat atcgggatcg
      961 gacggcagat cgccgaccgt cgcgctgacg gccagccgtc ctccgatgcg cgcgacgatg
     1021 tcgcgaaccg tatcggcgcc gagagccccg agcgcaccgg atgccggatc cttgcagtcg
     1081 atgatatccg cgccgcccgc aagcgccgcc tccgcttcgt ccgcgttggt cacgctggcg
     1141 agaaaggcgg gcttgcctag tctcaaaccg tcaatcattc cgttgttccg agatgggtac
     1201 gccgatggcc ctcaggccgc gaagatgcga ttttgttctg cgagcacggc ggcgcgatcg
     1261 tgacgctgtg cctcggtcag gttgatggtc acatcttgcg ccacactcgc ctttccgggc
     1321 gccaagcgca agatgcgcgt tccgagctga attgcctcgg tcctgtcgtg ggttacaaat
     1381 attaccgtgg tgggatggct gcgccaaagt tccatcagca gctcccgcag gctctgcgcc
     1441 gtgggatcgt ccagcgagac gaaaggctcg tccatcaaca ggatttcggg ctctaggata
     1501 aacccgcgcg ccagcgctgc gcggcgctgc atgccgagcg acaaccgctc cggataggcg
     1561 ttcgcggcct gtgtcaggcc aacgcgctcc agcatcgccg gaattttcac gtgcctcgga
     1621 tcaccgtcgg gaagaacgag cgcaatattc tcgtagaccg tccgccacgg caaaagccga
     1681 ggggtctgaa agatgaagg
//
LOCUS       DASWOG010000242         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_189309,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000242 DASWOG010000000
VERSION     DASWOG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189309"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGB93_04945"
     CDS             <1..369
                     /locus_tag="VGB93_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145491.1"
                     /translation="SMSAVTHIAPRAGFFDRMSFERILINLIDNANNSAPGRILVTTD
                     ETGGMLTLSVRDFGRGLDPKLQAKLTALVQPGGGAKDGFGLGLVVVRELAETNGGHVN
                     VENADPGLRVTATMKIKESA"
     gene            381..1076
                     /locus_tag="VGB93_04950"
     CDS             381..1076
                     /locus_tag="VGB93_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145492.1"
                     /translation="MSILIAEDDQHLRDGLVDLLSLEGYSCRVADDGAKALALFLQEK
                     PDICIFDVMMPVMDGLTLLREVRKLDTAVPILLLSARGEEGDRVRGLEIGADDYIGKP
                     FSARELVARIKAALRRTKVQAPMHEPEGRIHMGDIAIDSKAMRAFRGDHKIDLTNREV
                     AILAALFERRGQAVSRDELFDLAWGRDYMPNSRARDQDVSALRKKIELDPAAPRIIRT
                     VHGVGYRYDVDEA"
     gene            1083..>1564
                     /gene="recO"
                     /locus_tag="VGB93_04955"
     CDS             1083..>1564
                     /gene="recO"
                     /locus_tag="VGB93_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY0145493.1"
                     /translation="MEWHDDGLIIGLKKHGETSLILEAMTPAHGRHLGLVRGGRSKRW
                     QPLLQPGNSVALVWRARLDEHLGLYAVEVVKPRAANLMASALALHGLNHLATLLRLLA
                     ERDPHPALYETASTIADHLSETRTAAALVRFELAILAELGFGLDLATCAATGTREDLI
                     "
BASE COUNT          294 a          476 c          499 g          295 t
ORIGIN      
        1 tccatgagcg cggtcacgca tatcgcgccg cgcgccggat ttttcgaccg catgagcttc
       61 gagcgcattc tcatcaacct catcgacaac gccaataact ccgcgccggg gcgtattctc
      121 gtcacgaccg acgagacggg cggcatgctc acgctcagcg tgcgcgattt cggccgtggc
      181 ctcgatccca agctgcaagc gaaattgacg gcgcttgtgc agccgggcgg tggcgcgaaa
      241 gatggcttcg gcctcggcct tgtcgtcgtt cgcgaattgg ccgagaccaa cggtggccat
      301 gtgaatgttg aaaatgccga tccggggctg cgcgtaacag ccacgatgaa aatcaaagaa
      361 tcggcttagg aggaatggga atgtccatct tgatcgccga agacgatcaa catttgcgcg
      421 acggcctcgt cgatcttctg agcctcgaag gctatagttg ccgggtcgcg gatgacggcg
      481 ccaaggcgct ggctctgttt ctgcaggaaa agcccgacat ctgcatcttc gatgtcatga
      541 tgccggtgat ggatggtctg acgctgctgc gcgaagtgcg caagctcgat acggcggtgc
      601 cgatcctgtt gctttcggcg cgtggcgagg agggtgatcg cgtgcgcggc ctcgaaatcg
      661 gtgctgacga ttatatcggc aagccgttca gcgcgcggga actggttgcc cgcatcaagg
      721 cggccttgcg gcgcaccaaa gtgcaggcgc cgatgcatga acccgaaggc cgcatccaca
      781 tgggcgacat cgccatcgat tcgaaggcga tgcgggcgtt tcgcggcgac cataaaatcg
      841 atctgacaaa ccgtgaagtt gcgatcttgg ccgctctttt cgagcggcgc gggcaggccg
      901 tctctcgcga tgaattgttc gatctcgcct ggggccgcga ctacatgccg aacagccgcg
      961 cgcgcgacca agatgtctcg gccttgcgca agaagatcga gcttgatccc gccgcgccgc
     1021 gcatcatccg cacggttcac ggcgtcggct atcgctacga cgtggacgag gcctaggccc
     1081 gcatggaatg gcatgacgac ggactgatca tcggcctcaa gaagcacggc gagacaagcc
     1141 taatcctcga agcgatgacg ccagcgcatg ggcgccatct cggccttgtg cgcggcgggc
     1201 gttcgaagcg ctggcagccg ctgttgcagc ccggcaatag cgtggcgctc gtctggcggg
     1261 cgcggctcga tgaacatctc gggctgtatg cggtcgaggt ggtgaagccg cgcgccgcga
     1321 acctcatggc aagcgcgctc gcgttgcacg gtctcaatca tcttgccacg ctactgcgac
     1381 ttctggccga gcgcgatccg catccggcgc tttacgagac ggcatcgacg attgccgatc
     1441 atctcagcga aacgcgaact gccgccgcat tggtacgctt cgaactggcg atcctcgcgg
     1501 agcttggctt cgggcttgac ctcgcaactt gcgccgcgac cggcacgcgc gaagacttga
     1561 tcta
//
LOCUS       DASWOG010000243         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_78847,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000243 DASWOG010000000
VERSION     DASWOG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78847"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1502
                     /locus_tag="VGB93_04960"
     CDS             3..1502
                     /locus_tag="VGB93_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0145494.1"
                     /translation="MLFAAFSVHNDVEATGTVVKSALPYLLLGVALVTALGFEFVNGF
                     HDTANAVATVIYTRSLPAQVAVVWSGTFNFLGVLTSTGAVAFGIVSLLPVELILQVGS
                     EAGFAMVFAMLIAAIIWNLGTWWLGLPASSSHTLIGSIIGVGVANALMRGRDGTSGVD
                     WGKATEIGYALLLSPLVGFALSALMLLIFKALIKNPDLFKEPKGDQPPPAWIRGLLVL
                     TCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRAVPAHHIQTFVATSEAASKVV
                     DAKASGYNIIGDPRPAVTAYVSQHQLNEGTYPALAALIQDIGKQVQTYGSLAKVPANQ
                     VSNTRNDMYLASEAIRVLSKDKASSLSASDVKTLTAYKKELDLSTKFIPIWVKICVAL
                     ALGLGTMIGWKRIVVTVGEKIGKEHLNYGQGAAAEIVAMGTIFAADNFGLPVSTTHVL
                     SSGIAGTMAANGSGLQLATLRNIALAWVLTLPCAITLSATLYWIFRHIF"
     gene            1633..3018
                     /locus_tag="VGB93_04965"
     CDS             1633..3018
                     /locus_tag="VGB93_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0145495.1"
                     /translation="MAQIDAALAKIDANLDAALQRLFALVSIPSISTDPAYAGDCQNA
                     AEWLKDELHNLGFDASVRPTAGRPMVVAKAKARRTGAPHVLFYGHYDVQPSDPLDLWK
                     TPPFEPRLADALMGKQIVGRGTSDDKGQLMTFVEACRALKETGDLPCDITILFEGEEE
                     SGSHSLPAFLAENRDDLRADVALICDTSMLDRTTPAITTMLRGIVTQEVVIKGADRDL
                     HSGLFGGAAVNPIHVLAKVIADLHDENGRVTLPGFYDGVAELPAETAAQWRDIGFDGT
                     QFLKDVGLSVPVGEKGRSVLEMIWARPTCEINGIIGGYTGKGFKTVLPAQASAKISFR
                     LVGAQDPEKIAASFRAFVEAHLPADCRAEFISHGGSGALQLPFDSAPLTRARAALRDE
                     WGKEPVLIGSGGSIPIVGAFKRDLDMESLMIGFGLEDDRIHSPNEKYELSSFHKGARS
                     WARILTALGAA"
     gene            3210..>4439
                     /locus_tag="VGB93_04970"
     CDS             3210..>4439
                     /locus_tag="VGB93_04970"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY0145496.1"
                     /translation="MATDIEIARAAKLEPILKIAEKIGIPAEAVEPYGTKIGKVDLGF
                     LAEAQERAKSRAKSKLILVTAINPTAAGEGKTTTSVGLADALRHVGKKSVVALREPSL
                     GPCFGMKGGAAGGGYAQVVPMEAINRHFTGDFHAISAAHNLLAAMIDNHIYWGNQPAI
                     DSRRVALRRVVDMNDRALREIVVSLGGVANGYPRENGFDITVASEVMAIFCLARNLQD
                     LQERLGKIVVAYGPDKKPITAEDLKAAGAMTVLLKDALMPNLVQTLEGTPAFIHGGPF
                     ANIAHGCNSVIATTAGLALADYTVTEAGFGADLGAEKFMDIKCRKAGLAPSAAVIVAT
                     LRALKAQGGVVKADLGKENHAAVKAGLANLGRHVANVRKFGLPVVVAINHFGGDHEDE
                     LEIIAKTSRDEFGAEAIV"
BASE COUNT          807 a         1445 c         1342 g          845 t
ORIGIN      
        1 gactgctgtt cgctgctttt agtgtgcata acgacgtcga agcgaccggg acggtcgtca
       61 aaagtgcttt gccttacctc ctgcttggcg tcgctttggt gaccgccctt gggttcgaat
      121 tcgtcaacgg cttccacgat accgccaacg ctgtcgctac cgtcatctac acccgctcgc
      181 tgcccgcgca ggtcgccgtg gtctggtcgg gcaccttcaa cttccttggc gttctaacct
      241 cgaccggtgc cgttgccttc ggcatcgtct cgttgctgcc agtggagctg atcctccagg
      301 tcggctccga ggcaggtttc gccatggtgt tcgccatgtt gatcgccgcg atcatctgga
      361 acctcggcac ctggtggctc ggcctcccgg cttccagctc ccacacgctg atcggctcga
      421 tcattggcgt cggcgtcgca aacgcactca tgcgggggcg tgatgggacg tcgggcgtgg
      481 attggggcaa ggcgaccgaa atcggctacg cgctgctcct gtcgccgctg gttggcttcg
      541 cgctttcagc gcttatgctg ctgatcttca aggccctcat caagaatccc gatctcttca
      601 aagaaccgaa gggcgatcag ccgccgccgg cctggatccg cggccttctg gttctgacct
      661 gcacgggcgt ttcgttcgcg cacggttcga atgacggtca gaagggcatg ggccttatca
      721 tgctgatcct gatcggcacg gtgccaacag cctatgcgct caaccgcgcg gtgcctgcgc
      781 atcacattca gacctttgtc gccacctcgg aagcggcatc gaaggtcgtt gacgccaagg
      841 cgagcggcta caacatcatc ggcgaccctc gtcccgcggt gaccgcctat gtctcccagc
      901 atcagctcaa cgagggcacg tatcccgccc tcgccgcgct gatccaggac atcggcaagc
      961 aggtgcagac ctacggctcg cttgccaaag tgccggcaaa ccaggtctcg aacacccgta
     1021 acgacatgta cctcgcctcg gaagcgatcc gcgttctgag caaagacaaa gcgagcagcc
     1081 tttcggcgtc cgacgtgaaa acgctgaccg cctataagaa ggaactcgat ctctcgacga
     1141 agttcatccc gatctgggtg aagatctgcg tcgccttggc gctcggcctc ggcacgatga
     1201 tcggctggaa gcgcatcgtc gtcactgtcg gcgaaaagat cggcaaggaa cacctgaact
     1261 acggtcaggg tgctgccgcc gaaatcgtcg cgatgggtac gattttcgcg gccgacaact
     1321 tcggccttcc ggtctcgacc acgcacgtcc tctcgtccgg catcgccggc acgatggcgg
     1381 ccaacggctc gggactccag cttgccacgc tgcgcaatat cgccctcgcc tgggtgctca
     1441 ccctgccgtg cgcgatcacg ctctccgcaa cgctctactg gatcttccgc cacattttct
     1501 gatcggaagt tctctttgag caaagaggcc ggccctgccc ccggggccgg ctttttgttt
     1561 ggggcgagca ggcttgccgc ttcaacgcca agcggttaag ccagccggca tcccacacct
     1621 tatggaggcc gcatggctca gatcgatgcc gcgctggcga agatcgatgc gaatttggac
     1681 gcggcgttgc agcgtttatt cgcccttgtc tcgatcccgt ccatttcgac cgatccggcc
     1741 tatgccggag actgccaaaa tgcagcggaa tggctgaagg acgaattgca caacctcggc
     1801 tttgacgcct ccgtccggcc gaccgctggc cgccccatgg tcgtcgccaa ggccaaggcg
     1861 cgccgcacgg gcgcgccgca cgtgctgttc tacgggcatt acgacgtcca gccgtccgac
     1921 ccgctcgatc tctggaagac cccgcccttc gagccgcgcc ttgccgacgc gctgatgggt
     1981 aagcaaatcg tcggccgcgg cacctccgac gacaaaggcc agttaatgac ctttgtcgag
     2041 gcctgccggg cgttgaaaga aaccggcgat ctcccttgcg acatcaccat tctctttgag
     2101 ggtgaagagg aatcgggttc gcactcgttg cccgccttcc tcgcggagaa tagggatgac
     2161 ctccgcgccg acgtcgcgct gatctgcgat accagcatgc ttgatcgcac cacgccggcc
     2221 atcaccacca tgctgcgtgg aatcgtgacg caggaagtcg tcatcaaggg agcggatcgc
     2281 gaccttcatt ccggtctgtt cggcggcgct gcggtcaatc cgatccatgt gctggcgaaa
     2341 gtcatcgccg atctgcatga cgaaaatggc cgcgtcaccc tgcccggctt ttacgatggc
     2401 gtcgctgaat tgccggcgga gaccgccgcg caatggcgcg acattggctt cgacggcacg
     2461 caattcctca aggatgtcgg cctttccgtt ccggtgggcg agaaaggccg cagcgtgctc
     2521 gaaatgatct gggcgcggcc gacctgcgag atcaacggca tcatcggcgg ctataccggc
     2581 aagggattca agacggtgtt gccagcgcag gccagcgcaa aaatctcctt ccgtctcgtc
     2641 ggtgcccagg accccgagaa gatcgccgcg agcttccgcg ctttcgtcga ggcgcatctg
     2701 ccagctgatt gccgcgccga attcatttcg catggcggct ccggcgcgtt gcaattgccg
     2761 ttcgactcgg cgccgctcac gcgcgcccgc gccgcgttgc gcgacgaatg gggcaaagag
     2821 ccggtgctga tcggctcggg cggctcgatc ccgatcgtcg gggcgttcaa acgcgacctc
     2881 gacatggaaa gcctgatgat cggcttcggc ctcgaagacg atcgtatcca ttcgccgaac
     2941 gagaagtacg agctttcgtc atttcataag ggcgcgcgaa gctgggcacg cattcttacg
     3001 gcgctcggcg cggcataagt cagcgcgctc gaaatgctgc aatgcccgcg ctttggtgcg
     3061 gcgcagaagc gcgctctgcg tcaaactggg cattgacctt ttggcgcgtt gacggcagga
     3121 ccacgcttcg cccaagaagg cttatatgtt tccgccatca acaacttgcg cggcgtcaac
     3181 cagcgcgagg ccgaacccga gggaccgcca tggccaccga tattgaaatt gcccgcgctg
     3241 ccaagctcga acccatcctg aagattgccg agaagatcgg cattccggca gaggcggtcg
     3301 agccatacgg cacgaagatc ggcaaggtcg atctcggctt cttggccgag gcgcaggaac
     3361 gcgccaaaag ccgcgcgaag tcgaagctaa tcctcgtcac cgccatcaac ccgacggcgg
     3421 ccggcgaagg caagacaacg acctctgtcg gccttgccga tgcgttgcgc catgtcggca
     3481 agaagagcgt ggtcgccctg cgtgaaccat cgctcggccc ctgcttcggc atgaagggcg
     3541 gcgccgccgg cggtggctat gcccaggtcg tgccgatgga ggcgatcaac cggcacttca
     3601 ccggcgattt ccacgccatt tccgctgcgc ataatctgct cgcggcgatg atcgacaatc
     3661 acatctattg gggcaatcag ccagcgatcg actcgcgccg cgttgcgctg cgccgtgtgg
     3721 tggatatgaa cgaccgcgcg ctgcgcgaga tcgtcgtctc gctcggcggt gtcgccaacg
     3781 gctatccgcg tgaaaacggc tttgacatca cggtcgcttc ggaagtgatg gcgatattct
     3841 gcctcgcccg taaccttcag gatttgcagg agcggctcgg caaaatcgtc gtcgcctatg
     3901 ggcccgacaa gaagccgatc acggccgagg acctgaaggc cgccggtgcc atgaccgttc
     3961 tgctcaagga cgcgctgatg ccgaacctgg tgcagacgct ggaaggcacg ccggcgttca
     4021 tccatggcgg tcccttcgcc aatatcgcgc acggctgcaa ttcggtgatc gcgacgacgg
     4081 cgggcctcgc gctggccgat tacacggtga cggaagcggg cttcggcgcc gaccttggcg
     4141 cggaaaaatt catggacatc aagtgccgca aggcaggcct cgcaccatcc gccgccgtca
     4201 tcgttgcgac gctccgcgcg ctcaaggcgc agggcggcgt cgtcaaagcc gatctaggca
     4261 aggagaacca tgcggcggtg aaggccggcc tcgccaacct cggccgccat gtcgccaatg
     4321 tgcgcaagtt cggtctgccg gtcgtcgttg cgatcaatca tttcggcggc gatcacgagg
     4381 acgaactcga gatcatcgcc aagacctcgc gcgacgaatt cggcgccgag gcgatcgtc
//
LOCUS       DASWOG010000244         5671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_335488,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000244 DASWOG010000000
VERSION     DASWOG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335488"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VGB93_04975"
     CDS             complement(<1..190)
                     /locus_tag="VGB93_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0145497.1"
                     /translation="MITAFCLGFLLAGGGVAQAADTLRIAAQKTGTFSWELDVIKDHG
                     LDKKAGIDLDVTELATTEA"
     gene            374..775
                     /locus_tag="VGB93_04980"
     CDS             374..775
                     /locus_tag="VGB93_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0145498.1"
                     /translation="MKQNIIVFAAAGLFAMAGLAHPAYAAGDAAAGEQIFGHTCNLCH
                     QISPGAKNFVGPELNGLNGRKAGTAAGYDYSDAMKKSGITWGEDSFKKYITNPQADIP
                     GVKMIFAGLEKHSQRDNIWAYISQFKADGSK"
     gene            complement(850..1698)
                     /locus_tag="VGB93_04985"
     CDS             complement(850..1698)
                     /locus_tag="VGB93_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0145499.1"
                     /translation="MTSVTTTAPRRGFTFAQYMICMGGIIWREALRFVHQRGRFVSAL
                     VRPLVWLFIFAAGFRSVLGVSIIPPYETYILYDVYITPGLMAMIQLFNGMQSSLSMVY
                     DRETGNMRTLLVSPMPRWYLLTSKLLAGTAVSILQVYAYLLIAYFWGIEPNSLWGYLT
                     VLPALLLSGLMLGALGMALSSMVRQLENFAGVMNFVIFPMFFASSALYPIWKVQEGSP
                     WLATVCQLNPFTYAVELIRFALYLQVDWLSLAVVAGCLVVFLGAAILAYDPSRGMLIR
                     RGGAAE"
     gene            complement(1695..2459)
                     /locus_tag="VGB93_04990"
     CDS             complement(1695..2459)
                     /locus_tag="VGB93_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145500.1"
                     /translation="MQTSASPPALEVQNVTHFYGKRKALDNVSFNVAPGTFTVLLGLN
                     GAGKSTLFSLITRLYVTRTGTIKIFGYDVDRQSGEALRRLGVVFQARTLDLELTVTQN
                     LIYHAALHGIGPFEAKRRMADVLTRAGLADRAKERVRNLSGGLIRRVEIARAFLHQPQ
                     LLLLDEPTVGLDIKARADIISQVRSLINVDGVGVLWTTHLIDEIALTDSVVVLHQGKV
                     LDQGIASDIIARTGSPDMRSAFVKITGADISQGVAP"
     gene            complement(2638..3360)
                     /locus_tag="VGB93_04995"
     CDS             complement(2638..3360)
                     /locus_tag="VGB93_04995"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0145501.1"
                     /translation="MQILITRPAEQAYETAAVLSVLGYDAVLSPAVEIVPTDTPWPYG
                     TIDLLVATSARAFEALLTQPDFPTAEARRLMPLYVVGEKTLEAARLAAFAGPAVMADT
                     AKDLAPKLIAQLRSYTRALYLAGRERKPKLEKMCVDAGFTLDVLETYAAAAVAHLSDA
                     ALAALDDGAVGAVLHYSQRSAAIFLELLEAEGFDPAVLRHIAISEDAAIPLRELGLPH
                     IDVAAEPDENSMLALLPVGDRV"
     gene            complement(3376..4323)
                     /gene="hemC"
                     /locus_tag="VGB93_05000"
     CDS             complement(3376..4323)
                     /gene="hemC"
                     /locus_tag="VGB93_05000"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0145502.1"
                     /translation="MPDSLAAGALPARLRIGTRGSRLALTQADQVTKSIAAALGAQTP
                     LMEIIVIRTTGDQIQDRALGESGGKGLFTKELDSALMHGDIDLAVHSAKDLPTYLPQG
                     IDIVGYLPREDARDAWISNKAKDPRTLPSGSVVGTASLRRSAMLLRLRPDLAITLLRG
                     NVETRLAKIERGEIDATILALAGLKRLNFTDKATGLLDTHDFVPAVGQGAIAITVRSG
                     DAPVAAALKPILDAETAVALACERALLQVLDGSCRTPIGGHAWLENDVLHLRAIILRP
                     DGSQHFATQVQGSPADPQHLGESAARELLAQAPPGFLAV"
     gene            4489..5547
                     /gene="tsaD"
                     /locus_tag="VGB93_05005"
     CDS             4489..5547
                     /gene="tsaD"
                     /locus_tag="VGB93_05005"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0145503.1"
                     /translation="MRFLGIETTCDETAAAVVALRPEGGGEILSNEVFSQIAEHQAYG
                     GVVPEIAARAHIEVLDRLIVRALEDAKLELVDLDGIAAAAGPGLIGGVIIGLTTGKAL
                     ALAARKPFVAVNHLEAHALTARLAGGLDFPYLLLLVSGGHTQLVAVRGVGDYLRLGAT
                     VDDAVGEAFDKVAKMLGLPYPGGPQVEHEALKGDPTRFDFPRPMLGRPKPDFSFSGLK
                     TAVRLEAEKIAPLNPAAVADLCASFQAAVVDTLVDRLRAGIRVFGERVGPCQAMVVAG
                     GVAANSAIRRALMRFCGESGLRLIVPPVALCTDNGAMIAWAGIERLRLGLSDDMTFAA
                     RPRWPLDSDAETLHHGKA"
     gene            5548..>5671
                     /locus_tag="VGB93_05010"
     CDS             5548..>5671
                     /locus_tag="VGB93_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY0145504.1"
                     /translation="MPERTHIAVLGAGAWGTALANLAARNAAEVHLWAHDPAHVA"
BASE COUNT         1166 a         1732 c         1739 g         1034 t
ORIGIN      
        1 cggcttcggt cgtcgcaagt tccgtgacat ccagatcgat tccggccttt ttatcgaggc
       61 cgtgatcttt gatgacgtcc aattcccagg agaaagtgcc cgtcttctga gccgcgatcc
      121 gcagcgtatc ggcagcctgc gcgacgccgc cgccagcgag cagaaaaccg aggcaaaaag
      181 ccgtaatcat gcggagggaa tggttagacc cggcgaaccg aacgcaggaa agatcgttca
      241 tatggtgtga ctcactcatt cacaattgtg ttgcgcattg ccagaccgct gcagatcggg
      301 ctaccacatt tgttggaagc gctctaaatt ggcgcgcgct ggcgagaggc gcttggacta
      361 ggggaaggaa acgatgaagc agaacattat cgtctttgct gcggctggat tgtttgcgat
      421 ggccggactg gcgcatccgg cctatgccgc gggtgacgcc gctgccggtg aacagatttt
      481 tggccatact tgcaatcttt gccatcagat cagccccggc gcgaaaaact tcgtcggccc
      541 cgaactgaac ggcctcaacg gccgcaaggc cggcacggcg gccggctacg actattccga
      601 cgcgatgaag aagtccggca tcacgtgggg ggaagattcg ttcaagaaat acatcaccaa
      661 tccgcaggcc gatattccgg gcgtgaagat gatcttcgcg ggcctcgaaa agcacagcca
      721 gcgcgacaat atctgggcct acatttcgca gttcaaggcg gacggctcga agtaagccgg
      781 caacaacgtg ttgccaaagg aatcgaacgc tccggtcctc gccggagcgt ttttgttttg
      841 ccgcggcgct cattcggcgg ctccgccgcg gcggatgagc attccgcgtg aagggtcata
      901 agcgagaatc gcggcgccca ggaaaacgac caggcagccg gcgacgacgg cgagcgacag
      961 ccagtcaact tgcagataaa gcgcgaagcg gatcagttcg actgcgtagg tgaacggatt
     1021 gagctggcaa accgtcgcga gccacggtga gccctcctgc accttccaga tcggatagag
     1081 cgcgcttgag gcaaagaaca tcgggaagat gacgaaattc atcaccccgg cgaagttctc
     1141 aagctggcgc accatcgacg aaagcgccat gccaagcgcg ccgagcatca ggccggagag
     1201 cagcaacgcc ggcagcaccg tcagatagcc ccagagcgaa ttcggctcga tcccccagaa
     1261 ataggcgatg agcaaatagg catagacttg caggatcgac accgccgtcc cggccagcaa
     1321 tttcgaggtc aggagatacc agcgcggcat cgggctcacg agcaatgtgc gcatgttgcc
     1381 ggtctcgcgg tcgtagacca tcgaaagcga agattgcatg ccgttgaaga gctggatcat
     1441 cgccatcaga ccgggcgtaa tgtagacgtc gtagagtatg taggtctcgt acggcgggat
     1501 gatcgagaca ccgagaaccg agcgaaagcc cgcggcgaag atgaagagcc agacaagcgg
     1561 gcgcaccagc gccgaaacga agcggccgcg ctgatggacg aagcgcaacg cctcgcgcca
     1621 aatgatgccg cccatgcaga tcatatattg cgcgaacgtg aagccgcggc gcggcgccgt
     1681 cgtcgtcaca ctcgtcacgg cgcaactccc tggctgatat cggcgccggt gatcttgacg
     1741 aaggcgctgc gcatgtccgg gctgccagtg cgtgcgatga tgtcgctcgc gataccttga
     1801 tcgagcactt ttccttgatg cagcacgacg acggaatcgg tcagtgcgat ctcgtcgatc
     1861 agatgcgtcg tccacaaaac gccaacacca tcgacattaa taagactacg cacctgcgag
     1921 atgatgtcgg cccgggcctt gatatcgaga ccaaccgtcg gctcgtcgag cagcagcaat
     1981 tgcggttgat gtagaaaagc gcgggcgatc tcgacgcggc ggattaaacc gccagaaaga
     2041 ttccgcactc tctccttcgc acgatcggca agaccggcgc gcgtcagaac atcggccatg
     2101 cggcgcttgg cttcgaatgg gccaatgccg tgcagggcgg catgatagat cagattttgc
     2161 gtcaccgtca gttcgagatc gagcgtgcgc gcctggaaca cgacgccgag ccggcgcaaa
     2221 gcttcgccac tttggcgatc aacgtcataa ccgaaaattt tgatcgtgcc ggtgcgcgtg
     2281 acgtaaagcc gggtgatcaa cgaaaagagc gtactcttgc cggcaccgtt caatccaagc
     2341 agaacggtga aagtgcccgg cgcaacattg aaggacacat tatcgagagc tttgcgcttg
     2401 ccgtaaaaat gcgtgacgtt ctgaacttcg agcgcagggg ggctcgcaga cgtctgcatc
     2461 ggcactccgg cctcgttctg caacgcttct tccctctgaa ccgacattct tgccattcgt
     2521 attatgcgct gacgaccggg aaaaaccggc aatccttcgg gattttatca cgcgatatca
     2581 ggaaacatct atcaccgata catcatccac cataccagcc ggcttgtaag atatttgtta
     2641 gacgcgatct cccaccggca gcagagcgag catcgaattt tcgtccggct cggccgccac
     2701 gtcgatatgc ggcaggccga gttcgcgcaa tggaatggcc gcgtcctcgg aaatcgcaat
     2761 gtggcgcagc acggctggat cgaaaccctc ggcttcgagc aattccagaa aaatagctgc
     2821 gctgcgctgg gaataatgca gaaccgcacc gacggcgccg tcatccagcg cagccaacgc
     2881 cgcatcgctt aggtgtgcaa cagctgcggc ggcataggtt tcgagaacat cgagcgtaaa
     2941 accggcatcg acgcacattt tctcaagctt cggcttacgc tcgcgtccgg cgagatagag
     3001 cgcccgcgtg tacgagcgca attgcgcgat cagcttcggc gccagatcct tcgccgtgtc
     3061 ggccatcaca gcaggaccag cgaaggccgc aagacgcgcg gcttcgagag tcttctcgcc
     3121 cacgacatag agcggcatca gccggcgtgc ctcggccgtc gggaaatccg gctgcgtcag
     3181 caaagcctca aaagcgcggg cactcgtcgc cacgagaaga tcgatcgttc catacggcca
     3241 cggcgtatcc gtcgggacga tttcgacggc cggtgacaat actgcgtcgt agccgaggac
     3301 ggacagcaca gcggcagtct cataagcttg ttcagcaggc cgcgtgatca ggatctgcat
     3361 ggactgtcag cctcgtcata ccgccaaaaa gccgggcggt gcttgcgcga gaagttcgcg
     3421 ggccgcgctt tcgccgaggt gctgcggatc agcgggcgag ccttgaacct gcgtcgcgaa
     3481 atgttgcgag ccgtccggcc gcaagatgat cgcgcgcaaa tgcagcacgt cgttttcgag
     3541 ccaagcatga ccgccgatcg gcgtgcggca ggagccatcg agaacttgaa gcaacgcgcg
     3601 ttcgcaggcg agcgcaaccg ccgtttccgc atcaagtatc ggtttaagcg ccgccgcgac
     3661 cggcgcatcg ccactgcgaa cagtaatggc gattgcgccc tgcccgacgg cggggacgaa
     3721 atcatgcgtg tcgagcaatc ccgtcgcctt gtcggtgaag ttcagacgct tcagcccggc
     3781 gagggcgagg atcgtcgcat cgatctcccc acgctcgatc ttggcaagcc gggtttcgac
     3841 attgccacgc aaaagcgtaa ttgcgaggtc gggccgcagc cgcagaagca tcgcgctccg
     3901 ccgcaaggac gccgtaccga cgacgctccc cgacggcaac gtgcgtggat ccttcgcctt
     3961 gttggaaatc caggcatcgc gcgcatcttc gcgcggcaga tagccgacga tgtcgatgcc
     4021 ctgcggcaaa taggtcggca agtctttggc ggaatggacg gcgagatcga tgtcgccgtg
     4081 cataagcgcg ctgtcgagct ccttggtgaa gagccctttg ccgcccgatt ctccaagcgc
     4141 gcgatcctga atctgatcgc cggtcgtccg gatgacgata atttccatca gaggcgtctg
     4201 cgcgcccagc gccgccgcga tgcttttcgt gacttggtcg gcctgcgtta gcgccagacg
     4261 gctaccgcgc gtgcctattc tcagtcgcgc cgggagggcg cctgccgcaa gagaatcggg
     4321 aattgtcgat ttccttgtat tttggggtag atgtgaagaa cgaggcgtct tttgcacgaa
     4381 aagccggccc caagccagcc tgagggcttt agctgagacg agatcgatat tatccctttg
     4441 cggcgaggcg gtggcaccgg ccgtttcccc aagccattga gatcattaat gcgttttctc
     4501 ggaatcgaaa ccacctgcga cgagacggca gccgccgtcg ttgccctcag acccgagggc
     4561 ggcggcgaaa tcctctccaa cgaggtgttc agccagattg cggaacatca ggcctatggc
     4621 ggtgtggttc cggagattgc cgcgcgcgcc catatcgaag tgctcgaccg cctgatcgtg
     4681 cgggcgcttg aagatgccaa gctcgagctt gtcgacctcg acggaattgc cgccgccgcc
     4741 ggccccggcc tcatcggcgg cgtcataatc gggctgacca ccggcaaggc gctggcgctc
     4801 gcggcacgca aacccttcgt cgccgtcaac catctcgaag cccatgcgct caccgcgcgg
     4861 ctcgccggcg gtctggattt tccctatctg cttctgcttg tctcgggcgg ccatacgcag
     4921 ctcgtcgcgg tgcgtggcgt cggcgattat ctgcggctcg gcgcgacggt cgacgacgcg
     4981 gtcggcgaag ccttcgacaa agtcgcgaaa atgctcggcc tgccctatcc gggcggtcca
     5041 caagtggaac acgaagccct gaaaggcgat ccgacgcgtt tcgactttcc gcgcccgatg
     5101 ctcgggcgtc cgaagcccga cttctcgttc tcgggtttga aaaccgccgt gcggctcgaa
     5161 gccgagaaaa tcgcgccgct caatcctgcc gcggtcgccg atctttgcgc ctcgtttcag
     5221 gccgccgtgg tcgatacgtt ggtcgatcgg ctacgcgccg gcatccgcgt cttcggcgaa
     5281 cgcgttgggc cctgtcaggc gatggttgtc gctggtggcg tcgctgccaa ttcggcgatc
     5341 cgacgcgccc tgatgcgctt ttgcggcgag agcggcctgc ggctgatcgt gccgccggtc
     5401 gcgctttgca ccgacaatgg cgcgatgatc gcgtgggcgg ggatcgagcg gctgcggctc
     5461 ggcctcagcg acgacatgac cttcgccgcg cggccgcgct ggccgctcga tagcgacgcc
     5521 gaaactctcc accacggcaa agcctgaatg cccgagcgaa cgcatattgc cgtgctgggt
     5581 gcaggcgcct ggggcacggc gctcgccaat ctcgccgccc gcaatgcagc cgaggttcat
     5641 ctttgggcgc atgatcctgc ccatgttgct g
//
LOCUS       DASWOG010000245         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_78867,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000245 DASWOG010000000
VERSION     DASWOG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78867"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGB93_05015"
     CDS             complement(<1..225)
                     /locus_tag="VGB93_05015"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0145505.1"
                     /translation="MTVNGWIQIALYSVVIIALTKPVGFYLTRVFSGERTFLSPILRP
                     VERAIYAVCGVNEAEEQHSVTYAISMAFFSA"
     gene            complement(237..326)
                     /locus_tag="VGB93_05020"
     CDS             complement(237..326)
                     /locus_tag="VGB93_05020"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEY0145506.1"
                     /translation="MIIDYTLGGLAVIFILGYLVYALVRPERF"
     gene            643..1965
                     /gene="hemN"
                     /locus_tag="VGB93_05025"
     CDS             643..1965
                     /gene="hemN"
                     /locus_tag="VGB93_05025"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY0145507.1"
                     /translation="MSSFRALAERNVPRYTSYPTAPHFSAAIGPGEYAQWLGALAPDA
                     RLSLYIHVPYCSDICLYCGCHTKAVHQRAPVDFYAERLSDEIARLAEIAAGRRVTHLH
                     WGGGTPSILGAYWLRQIVQSLDASFDLSEIKEHAIEIDPRHISTELTQTLAEIGVNRA
                     SLGVQDFSPHVQRAIGRIQPFEKVAHAVGLLHEVGISNINIDLMYGLPHQSADDVDAS
                     AQLAALLSPQRIALFGYAHVPWLKANQKCIDEAVLPGVVVRLAQAQKAAETLAAFGYV
                     AIGLDHFALPDDPLAVAAREGRLHRNFQGYTVDDAEALIGLGASAIGKLPQGFVQNAV
                     DMAGYMRRIDGASLATTKGFATSVEDERRAMIIERLMCGLAVDLSELAGDYSAEMESL
                     RPMIQDGLVEFDGSRLAITKRGRPFSRIVAAAFDAYLPANAARHSMAV"
BASE COUNT          422 a          564 c          619 g          409 t
ORIGIN      
        1 cgcgctgaag aacgccatcg agatcgcata cgtgaccgaa tgttgctctt cggcttcgtt
       61 gacgccgcag accgcgtaga tggcgcgttc aaccggccgc aggatgggcg aaaggaaagt
      121 ccgttcgccc gaaaaaacac gcgtcagata gaagccgacc ggcttcgtca acgcgatgat
      181 caccactgag tagagggcga tctgaatcca tccgttcaca gtcatagcaa aaactcctag
      241 aagcgttcgg gacggacgag cgcgtagacg aggtaaccca ggatgaagat gacggccaag
      301 ccgccgagcg tgtaatcgat gatcatggtc ggctcacaat ctgtcgcagg cgaaaacgta
      361 aagggtgagg atcagaaacg cggcaatgcc gatcgcgaga taaagaatgt caaacatgcg
      421 gacgacctcc gtggcgcgcg ctagaaagag ccagcgggtg atcgcaggac acgatctgcc
      481 gccttcggct cgtggtcgtg atgtggccca aggcgcataa aggttcgagc ggtcttcgcg
      541 gctgcggaaa tataaatttc atataaatgc ggggtgcggc ttccccctgg cggctttgac
      601 gcagatcaaa gccgcgtcag gatttgcagc tacggttcga caatgtcttc gttccgtgcc
      661 ctcgctgaac gcaacgtacc gcgctacacg tcttacccga cggcgccgca tttttctgcc
      721 gcgatcgggc ctggggaata tgcgcaatgg cttggtgcgc tcgcaccgga tgcgcggctc
      781 tccctttata ttcatgtgcc ctattgttcg gacatctgcc tctattgcgg gtgtcacacc
      841 aaggcggtgc atcagcgcgc gccagtcgac ttctatgccg agcggctcag cgacgagatt
      901 gcgcggctcg cggagatcgc ggccgggcga cgcgtgaccc atctgcattg gggcggcggt
      961 acaccgtcga tcctcggggc atactggctg cgccagatcg tccaaagcct agacgcgtca
     1021 ttcgatcttt cggagatcaa ggagcacgcg atcgaaatcg atccgcggca tatttcgacg
     1081 gagttgacgc aaacgctggc cgagattggc gtcaatcgcg ccagtctggg tgttcaggat
     1141 ttctcgccgc atgtgcaaag ggcgattggc cgtatccagc cgttcgaaaa ggtcgcacat
     1201 gccgtcggcc tgttacacga ggtcgggatt tcgaatatca atatcgatct gatgtatggt
     1261 ttaccgcatc aaagtgccga tgatgtcgac gcgagcgcac aactcgccgc tttactttcg
     1321 ccgcaacgga tcgcactgtt cggctatgcg catgtacctt ggttaaaggc aaatcagaag
     1381 tgcatcgatg aggcagtcct gcctggcgtc gtggtgcggc tcgcgcaagc gcaaaaggca
     1441 gccgagacgt tggcagcctt cggttatgtc gcgatcggtc ttgaccattt cgcgttgccc
     1501 gacgatccac tggcggtcgc cgcgcgtgaa ggccgtctcc accgcaattt ccagggctat
     1561 acggtcgatg acgcggaggc gttgatcgga ctcggtgcgt cagcgattgg caagctgcca
     1621 caaggttttg tgcagaacgc tgtggacatg gcaggctata tgcgccgcat tgatggcgcc
     1681 agcctagcca cgacgaaggg ttttgccacc tcggtcgaag acgagcgccg ggcaatgatc
     1741 atcgagcggc taatgtgcgg tcttgcggtg gacctcagcg aactcgcagg tgattacagt
     1801 gcggagatgg aatcgttgcg gccgatgata caggacggac tggtggagtt cgacggttcg
     1861 cggctcgcaa ttaccaagcg cggccgcccg ttctcacgga tcgtcgctgc tgctttcgat
     1921 gcgtatctgc cggcaaacgc cgcacggcat tctatggcgg tataaaaacg cagcctattc
     1981 gcccgtggcc gctatcggca gtacagcgac attc
//
LOCUS       DASWOG010000246         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_42034,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000246 DASWOG010000000
VERSION     DASWOG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42034"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VGB93_05030"
     CDS             <1..1280
                     /locus_tag="VGB93_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0145508.1"
                     /translation="HGGWRADPTTGSVAVPIHQTSSYLFHDTEHAENLFGLQELGFIY
                     TRIGNPTNDVLEKRVAALEGGAAALAVASGQAASAYALQNIASAGDNIVSATDLYGGT
                     WNLFANTLKSQGIEVRFVDPSDPENFRHATDERTRAYYLETLPNPKLVVPPIAEIAAI
                     GREQGIPLVVDNTATPLIARPLQHGAAIVVYSATKYLGGHGNSIGGIIVDGGNFDWQK
                     HPQRQPALNTPDPSYHGTIWTEAAKPIGPIAYILRARTVLLRDLGAALSPFNAFLILQ
                     GIESLPLRIERHVENAGKVVEYLRQRPEVARVIHPSRQTGAERARADKYLNGQYGGLV
                     GFELAGGRAAGRRFIDGLELFYHVANIGDSRSLAIHPATTTHSQLSAEDRLATGVSDD
                     YVRLSVGLEHIDDIIVDLDRGFAAARIFAQAAE"
     gene            1334..>1799
                     /locus_tag="VGB93_05035"
     CDS             1334..>1799
                     /locus_tag="VGB93_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY0145509.1"
                     /translation="MASEKARVTLVSSSPTPLRQVFSDDNLWERLRQEAAEVLTREPI
                     LATLILTTIINRDTFEEAVIHRVALRLANSTVSTDLIVDAFTAALRSDPEIGKAFRAD
                     IAAVVDRDPATARLLEPLLYYKGFHAIQTHRLAHYLWNANQRDLAFYLQSRSS"
BASE COUNT          339 a          578 c          509 g          373 t
ORIGIN      
        1 tgcatggcgg ctggcgggcc gacccgacca cagggtcggt cgccgtcccg atccatcaaa
       61 cttcgtcata tctgttccac gataccgagc acgccgaaaa cctgttcggc ttgcaggaat
      121 tgggtttcat ctacacgcgt atcggtaacc cgaccaacga cgtattggag aagcgcgtcg
      181 cagcgttgga aggtggggcc gcggcgctcg ccgtcgcttc aggccaagcg gcctcggcct
      241 acgcgctgca aaatatagcg tctgccggcg acaacatcgt ctccgcgacc gacctctacg
      301 gcggcacctg gaatcttttc gccaatacgc tcaagtcgca gggaatcgag gttcgcttcg
      361 tcgatccctc agaccccgaa aacttccgcc acgccaccga cgagcgcacg cgcgcctatt
      421 atcttgagac cttgcccaat ccgaagctcg tcgtgccgcc cattgccgag atcgccgcga
      481 tcggccgcga acaaggtatt ccactcgtcg tcgataacac tgcaacgccg ctcatcgcgc
      541 ggcctttgca gcatggggca gcgatcgttg tttattccgc aacgaaatat cttggcggcc
      601 acggcaattc gatcggcggc atcattgtcg atggcggcaa tttcgactgg cagaaacatc
      661 ctcaacgcca gcctgccctc aacacgccgg acccgagcta tcacggcacg atctggacgg
      721 aggccgcaaa accaatcggt cctatcgcct atatcttgcg cgcgcggacg gttctgttac
      781 gcgacctcgg cgccgcgctc tcgcctttca atgcattcct catcctgcaa ggcatcgaat
      841 ccttgcccct gcgcattgag cggcatgtcg agaacgccgg caaagtggtc gaatatctgc
      901 ggcagcggcc ggaggttgcg cgcgtcattc atccttcgcg gcagacaggc gccgagcgcg
      961 cccgtgccga caaatatctg aacggccaat atggcggcct cgttggtttt gaattggcag
     1021 gcggccgcgc ggcggggcgg cgctttatcg atgggctcga actattttac cacgtcgcca
     1081 atatcggcga ttcccgcagt ctcgccattc atccggcgac gacgacccac tcgcaattgt
     1141 cggccgaaga tcggcttgcc acaggtgtct ctgatgatta tgtccggctg tcggtcggcc
     1201 ttgagcatat cgacgatatc atcgtcgatc tcgatcgcgg ttttgccgcc gcccgcatat
     1261 ttgcgcaggc ggcagaatga gtgagcgcga agggattttg tcttcgttat tatcggtgaa
     1321 cgggatttcg cctatggcct cggaaaaagc gcgcgtgacg ctcgtctctt cttcaccgac
     1381 gccgttgcgg caagtctttt cggacgacaa tctttgggaa cgccttcgcc aggaggcagc
     1441 cgaagtgctc acgcgcgagc caatcctcgc gacattgatc ctgaccacca tcatcaatcg
     1501 cgacacgttt gaggaggcgg tcattcaccg cgtcgcgttg cggctcgcga atagcacggt
     1561 ctcgaccgat ctgatcgtcg atgcgttcac ggcagcgctg cgcagcgatc cggagatcgg
     1621 caaggctttt cgcgccgaca tcgcggcggt tgtggatcgc gatcctgcaa ccgcgcgcct
     1681 gcttgagccc ctgctctatt acaagggttt ccatgcgatc cagacccaca ggctggcgca
     1741 ttatctgtgg aacgccaacc agcgcgatct cgccttctat ctgcagagcc gctcgtcgg
//
LOCUS       DASWOG010000247         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_299388,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000247 DASWOG010000000
VERSION     DASWOG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299388"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VGB93_05040"
     CDS             <1..857
                     /locus_tag="VGB93_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0145510.1"
                     /translation="LSFFAAELTSDDGWDAAVAGCDYVLHVASPLGRDDPKDPNELII
                     PARAGTLRVLKAATRAGVERVVFTSSAATCRPPHNSPDSVSDETVWTDPTEPRLGAYP
                     LSKTLAERAAWDFMATQPSTRTTLTTILPTAVLGPLLTAENLGSVQLLQRLMNGSMPG
                     YPRLGFGVVDVRDVADIHIRAMTAPEAAGQRYLAISEWLWMKQMAEIGRSGLGSRAKV
                     PTRQMPDFVLRLISIFDRPLRFIIPALGKKHANTSAKAQAVLGWKPRPVRETIVDCAE
                     SLIAKGAV"
     gene            complement(1130..>1674)
                     /locus_tag="VGB93_05045"
     CDS             complement(1130..>1674)
                     /locus_tag="VGB93_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145511.1"
                     /translation="KYGRADATDDEVYAAAKLAQIDDFVRALPAGYDTMVGERGLKLS
                     GGEKQRVAIARTILKAPPILLLDEATSALDSHTEKEIQDALDRVSKNRTTVVIAHRLS
                     TIVHADTILVFEAGRVAEQGTHAELIAKNGLYASLWARQRQAEKAREVLAHALEEAGE
                     SPPRFGSSEEESTLARTGTA"
BASE COUNT          295 a          547 c          505 g          327 t
ORIGIN      
        1 ggctttcgtt tttcgccgcc gaactgacaa gcgacgacgg atgggatgct gctgtcgcgg
       61 gctgcgatta cgttcttcac gtcgcttctc cgcttggcag agatgatccc aaagatccca
      121 acgagctgat catccccgcg cgcgcgggca cgttgcgggt gctgaaggcg gcaacgcgcg
      181 ctggcgtcga gcgtgtcgtg ttcacgtcct ccgcggccac atgccggccg ccgcacaaca
      241 gtcccgatag cgtaagcgac gagaccgtct ggaccgaccc gacggaaccg cgtctcggcg
      301 cttatccgct ctccaagacg ttggccgagc gcgctgcgtg ggatttcatg gcaacgcagc
      361 catcgacccg aacgaccctg acaacgatcc tgccgacagc cgttctcggg ccgctgctga
      421 cggcggagaa tctagggtcc gtgcagcttc tccagcgact gatgaatggc agcatgccgg
      481 gctacccgcg cctcggtttc ggtgtcgtcg atgtgcgcga cgtcgccgat attcacatcc
      541 gcgccatgac ggcgccagaa gccgcgggcc agcgctacct cgctatcagc gaatggcttt
      601 ggatgaagca gatggccgag atcgggcgct caggcttggg gtcgcgtgca aaagttccga
      661 cgcggcaaat gcccgacttc gtcttgaggc tgatttccat cttcgatcga cccctccgct
      721 tcatcatccc cgcgctcgga aaaaagcacg cgaacacctc tgcgaaagcg caagccgtct
      781 tgggctggaa accgcgcccc gtgcgcgaga cgatcgtcga ctgtgccgag agcctcatcg
      841 ccaaaggcgc cgtgtgagcc accaaacctc tgttgtcatc ctcgggcttg tcccgaggat
      901 tcaggccgcc gcaaatcttg tgataaccca gccctatatc gtcatcccgc gcaggcgggg
      961 atccgtccaa gtgtcagcaa cgcgacgaac atcgatctgg atggatccca gcctccgccg
     1021 ggatgacgtt gggagagatt gagactctct actttcatca tcttcgggct cgtcccgagg
     1081 atccattttc catccgcgct tttgagatgg aaacccgagg atgacaatct taggctgtgc
     1141 cggtccgcgc taacgtcgat tcctcttctg aggaaccgaa ccgcggcggc gattccccgg
     1201 cttcttccag agcgtgggcg aggacctcgc gcgctttctc ggcttgccgc tgacgcgccc
     1261 agagacttgc ataaagccca ttcttggcga tgagctcggc gtgcgtccct tgctccgcga
     1321 cgcgcccggc ctcgaacacc aaaatcgtgt cggcatgaac gatcgtcgac aaccgatgcg
     1381 cgatgacgac cgtcgtccgg ttctttgaaa cgcgatcgag cgcgtcctgg atttcctttt
     1441 ccgtgtggct gtcgagcgcg ctcgtcgctt cgtccagcag caggatcggg ggcgccttca
     1501 aaatcgtgcg cgcgatcgcg acgcgctgct tctcgccgcc ggaaagcttg aggccgcgct
     1561 cgccgaccat cgtgtcgtag ccggcgggga gggcgcgaac gaaatcgtcg atctgagcga
     1621 gcttcgccgc cgcatagacc tcgtcgtcgg tggcgtcggc gcggccatac ttga
//
LOCUS       DASWOG010000248         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_262596,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000248 DASWOG010000000
VERSION     DASWOG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262596"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VGB93_05050"
     CDS             complement(<1..1486)
                     /locus_tag="VGB93_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0145512.1"
                     /translation="MSAASSHDETAEERFRALVSGYRPLANVPDEFIGPDGQPRSHWL
                     KFFHALSELGADEIDRRFATADRNIRDTGVSYRAYADTSERAWPLSHLPLLIEADEWH
                     AIAAGVTQRARLLEAVARDIYGPAELVENGDLPAAAVTGSSDYLPQLQGLQPTAGNFL
                     YLYAADIGRGPDGNWWVIGDRTQAPSGAGYALANRLVQSRAFPALYRDMNVKRLAPFF
                     QAFRDGLIGAASHADPRICLLTPGPYNETYFEQAYLARYLGFLLVEGGDLTMRDNEIN
                     VRTIAGLKRADVIWRRIDSDYADPLELNARSRLGVPGLVDTLREGGVVMANALGSGVL
                     EAPVMMSFLPRLCRRILGEDLLMPNVATWWCGQPRERERVLARPERMAIAGAFGNPIL
                     GHPANQAIVGASLTPDEKRQLLDDIERRGVDYVGQEVVNLSTTPVWEGGKLEPRPFVL
                     RVYAARTRDGWSIMPGGFCRISGRPDARAVSMGEGVRSADVWVLS"
     gene            complement(1489..2034)
                     /gene="mog"
                     /locus_tag="VGB93_05055"
     CDS             complement(1489..2034)
                     /gene="mog"
                     /locus_tag="VGB93_05055"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY0145513.1"
                     /translation="MPDKHKDTALIGIVTASDRASQGIYRDESGPAIVAYLNRTLTSP
                     FNIAQRVIPDGFESVRDTLINLADDEGCDLILTTGGTGPAPRDLTPEATLAVAPRELP
                     GFGELMRAESLKQVPTAILSRQIAAHRGACLIINLPGKPAAIETCLNAVFPAVPYCLD
                     LIGAARLETDPTVVKSFRPKS"
     gene            complement(2076..2282)
                     /locus_tag="VGB93_05060"
     CDS             complement(2076..2282)
                     /locus_tag="VGB93_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0145514.1"
                     /translation="MPPRISQHSLDEVLKEQAAVSEALQDMIDVVTEPWGVKVERVQM
                     KDVEIPDTMQRHRRRHREARMTIA"
     gene            complement(2359..3510)
                     /locus_tag="VGB93_05065"
     CDS             complement(2359..3510)
                     /locus_tag="VGB93_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145515.1"
                     /translation="MNIAQPLEALAARREALTPYSLDTLVTGAARLRPDGLAFADRLG
                     PWPFGIVAAQVSALARILADCGLQPGERILIVGGAEVSVVTAAIAALRGGFVPALAPI
                     DLDAAALAAYGQAIDAAAIAGPTSYGDFASFETCLAAAAAMPSVRLVATLGPTEIDGA
                     VDLSLGAMLKYAAQHPDFGLERGKPLPEPPPVITYDRNRARPVHHRQAALIAAGLDFV
                     ARARIGRVTPILSTLAPVSAAGFIAGPCAALLSGAPLYLHGPFSEEDFLAARNQADSA
                     HLILPAGLAGDFVGIFDGLASVVLVSRLAAEQPYTPPPAIEAPCPVVDLYAVDETSVI
                     AEPRRNHIPVSPAQEPHFVGFDEARVLTFEKAADSHALAFKGAGVTAAD"
     gene            complement(3507..5381)
                     /locus_tag="VGB93_05070"
                     /pseudo
     CDS             complement(3507..5381)
                     /locus_tag="VGB93_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT         1079 a         1620 c         1772 g          924 t
ORIGIN      
        1 cggacagcac ccagacgtcg gccgagcgca cgccttcacc catcgaaacg gcgcgagcat
       61 cggggcgtcc ggaaatgcgg cagaagccgc cgggcatgat cgaccagccg tcacgggtgc
      121 gcgccgcata gacgcgcagg acaaacgggc gcggttcgag cttgccgcct tcccacacag
      181 gcgtcgtcga aagattgacg acttcctggc cgacgtaatc gacgccgcgc cgctcgatat
      241 cgtcgagcaa ttggcgcttc tcatccggcg tcagcgaggc gccgacaatc gcttggttcg
      301 ccggatggcc gagaatggga ttgccgaaag cgccggcgat cgccatgcgt tcgggacgtg
      361 caagaacgcg ctcgcgttca cgcggctggc cgcaccacca ggtcgcgaca ttcggcatca
      421 gcaggtcttc gccgagaatg cgccggcaga gacgcggcag gaagctcatc attaccggcg
      481 cttcaagaac gccggagccg agcgcgttcg ccatcaccac gccgccctcg cgcaatgtat
      541 cgacgagccc cggcacgcca agccgcgagc gcgcgttgag ttcaagcgga tcggcataat
      601 cggaatcgat acgccgccag atcacgtcgg cgcgtttcag gccggcaatg gtgcggacgt
      661 tgatctcatt gtcgcgcatg gtgagatcgc caccctcgac cagcagaaaa ccgagataac
      721 gcgcaagata ggcctgctcg aaataggtct cgttgtaggg gccgggcgtc agcaggcaga
      781 tgcgcggatc ggcatggctc gcggcgccga tgaggccatc gcgaaaggcc tgaaagaacg
      841 gcgccagccg tttgacgttc atgtcgcggt agagcgccgg gaacgcgcgc gattgcacca
      901 gcctgttggc cagcgcatag ccggcgccgg acggcgcctg cgtgcggtca ccgatcaccc
      961 accagtttcc gtccggcccg cggccaatgt cggcggcata gagatagaga aaatttccag
     1021 ccgtcggctg caggccctgg agttgcggca ggtagtcact gctgccggtg acggccgcgg
     1081 ccggcaggtc gccgttctcg acaagctcgg cggggccgta aatgtctctg gcaaccgcct
     1141 ccagcagacg cgcgcgctgc gttacgcctg ctgcaatggc gtgccattcg tccgcctcga
     1201 tcagcagcgg cagatggctc aaaggccacg cgcgctcgct cgtgtcggca tacgcacgat
     1261 aagagacgcc agtgtcgcgg atgttgcggt cggcggtcgc gaagcggcgg tcgatctcgt
     1321 cggcgccaag ttcgctcaac gcatgaaaga atttcagcca atgtgaacgc ggctgaccgt
     1381 ctggcccgat gaattcgtcc ggcacattgg cgagcggccg atagccgctg acaagcgcgc
     1441 ggaaacgctc ctcggcggtt tcgtcgtgag aggaagctgc gctcatcgtc atgatttcgg
     1501 ccggaaggat ttgacgacag tggggtcagt ttcgagccgc gccgcgccga tcagatcgag
     1561 gcaatagggc accgccggaa acacggcgtt gagacaggtt tcgatcgccg ccggtttgcc
     1621 cggcagattg ataatgaggc aggcgccgcg gtgcgcagcg atctggcgcg acaggatcgc
     1681 ggttggcacc tgtttcaagc tttctgcgcg catcaattcg ccgaaaccag gcaattcgcg
     1741 cggcgcgacg gccagcgtcg cttccggcgt cagatcgcgc ggtgcaggcc ccgtgccgcc
     1801 cgtggttagg atcagatcgc aaccttcgtc gtcggcaaga ttgatcagcg tatcgcgaac
     1861 ggattcaaag ccgtccggaa tgacgcgttg cgcgatgttg aagggcgagg tcagggtgcg
     1921 attcaaatag gcgacaatcg ccggtccgct ttcatcgcga tagatgccct ggctcgcccg
     1981 gtcggatgcg gtgacgattc cgatgagcgc ggtatcttta tgtttatctg gcatttgaat
     2041 cgtgttcgat caatgatccg cgctcggtcg caagcttatg caattgtcat tctcgcttca
     2101 cggtgccggc gccgatggcg ctgcatagtg tcgggaattt cgacgtcctt catctgcacg
     2161 cgttcgactt tcacgcccca aggctcggtc acaacatcta tcatgtcctg gagggcttcg
     2221 gagaccgctg cctgttcttt caacacttca tcgagggagt gttggctaat ccgcggcggc
     2281 aaagaggatc gcggcgagaa tgaacatgac gcgcgtcctc cgcggccccg gcgattggcg
     2341 ggtgcggact atgcccgatc aatctgccgc cgtcacgccc gcgcctttaa aagcgagtgc
     2401 gtgagaatcc gctgcctttt caaaggtcag gacgcgggct tcgtcgaaac cgacgaaatg
     2461 gggctcctgc gctggtgaca cgggaatgtg attgcggcgc ggttccgcga tgacgctcgt
     2521 ttcatccacg gcgtagagat cgacgaccgg gcagggcgct tcgatcgctg gcggcggtgt
     2581 ataaggttgc tcggccgcaa gccgcgatac gagcacgacg gaggcgaggc cgtcgaaaat
     2641 gccgacgaaa tcaccggcca gtccggccgg taaaataaga tgcgcggagt cggcctgatt
     2701 acgggctgca agaaaatctt cttccgaaaa tggcccatgc agatagagcg gggcgccgga
     2761 gagaagggcg gcgcaaggcc cggcaatgaa gcccgccgcc gaaaccggtg ccaacgtgga
     2821 caggatcggc gtgacgcggc cgatgcgggc gcgggcaaca aagtcgagcc cggcggcgat
     2881 cagagccgct tgccgatgat gcaccggccg cgcacgattt cgatcatagg tgatgacggg
     2941 cggcggttcc ggcagcggct tgccgcgttc aagtccgaaa tcgggatgct gcgcagcata
     3001 tttcagcatc gcgccaaggc tgagatcgac agccccgtca atttccgtcg gcccgagggt
     3061 cgcgaccaac cggaccgaag gcatcgcggc tgcggccgcc aggcaggttt caaacgaggc
     3121 gaaatcgcca tagctggtcg gcccggcgat ggcggcggcg tcgatcgcct gcccgtaggc
     3181 ggctaaggcc gcggcgtcga gatcgatcgg cgccaacgcg ggcacgaagc cgccgcgcaa
     3241 agccgcaatt gcagccgtta cgactgagac ttccgcgccg ccgactatga ggatgcgctc
     3301 accgggttgc agaccgcagt cggcgagaat gcgcgccaaa gccgagactt gcgcggcgac
     3361 gattccgaaa ggccacggcc ccagacgatc ggcaaaggcg agaccatccg gccgcagccg
     3421 cgccgcgccc gtcacgaggg tgtcgagact atagggcgtc aacgcctctc ggcgggcggc
     3481 caaggcttcg agaggttggg caatgttcac ggcttcttcc tccaccagga atctagcgcg
     3541 tcatcgagtg ttggagaacc gtaggcgggc aatttcgcgg gtctggcaag cgtcgtggag
     3601 gtcgcaatcc actgatttcg cgcgtggtag agggggacaa tataaaaacc ggacaaaagc
     3661 acccggtcga gcgcgcgcac actcatcaca aaatcgtcct gtgattgagc cgcgagcaat
     3721 gagtcgacga ccgtatccac cgcgggcgat tcgacaccgg caaggttgta ggacgccggc
     3781 tcgctggccg cctccgaacc ccagcgggtg cgctcctcgc tgcccggcga cgcggaggcg
     3841 agccacgagc cgatcatcat gtcgaaatcg aaattctgcc gccggcgctc gtattggacc
     3901 tcatcgacga gacgcacgcg cgcgtcgacg ccaatgcggg cgagagaatc cgcataattg
     3961 agggccagac gctcctgcaa tcgatcctgg accatgatct cgaaacgaag ttgctggcct
     4021 gtgtggatca gcttgccgtc gttcatctga tagcccgcag agccaagcag cgtcagggcg
     4081 cgaaacggcc agattcgatc gtgccccgag ccgtcggttt gcggcggctg ccagagacct
     4141 tcgagaatgt cgggccgcac ggcgcgcggg aacggcttca gcagagcgcg ttcggcgagg
     4201 ctcgtgggac gctcggtcta catgaagatc gaatcgtcga agaagctttt cgtgcgcgtg
     4261 tagagaccgc agaaaagatt ggcgttgacc cattcaaagt cgaacgccat gcccagcgcc
     4321 tcgcgtacgc gaatgtcctt gaagagatcg cgccgcagat tgaagacaaa accatccatg
     4381 cctttcgggc cgccgattgg caggctttca atggcgtcac gatgcgcacg cacggcggga
     4441 aaatcgtatc ccgttgtcca gcgcaaagga tcggtttcga tgcggaaatc gacgaggccg
     4501 gtgcggaatg cctcaaaaag gcttgtcggg tcccgataat aatcgatctc gaccgcgtcg
     4561 aagttgaaaa acccgcgctg gctgggcagg tccttcgccc aataattggg attgcgcttc
     4621 aaaatgagct tttggcccgg atcgacagtc gcgatccggt aggggcccga gccgatggga
     4681 atatcgaaac tcgcggattc gaaattggcg gcgtcggtcg ccttgtgcga aagcaccggc
     4741 atcaatgcga ggatcagcgg aagttcacga tcgccgagac cgggaaaatc aaagcggatc
     4801 gtttcgtcgt ccggcgtttc cacgcttttg acgagcgcat aggcgctgcg ttgttgcggg
     4861 cgccccttcg tcttcagcag atcgaaggtg aaacgcacgt ccgccgcatt gattgggctg
     4921 ccatcggaaa aacgcgcgcg cggatcgaga tggaagagca cgtaatcgcg cgcggcgtcg
     4981 gtctcgacgc ttttagcgat cagcccgtac atcgtgaacg gctcgtccat cgaacgcgtc
     5041 atcaggctct ggaaaacgtc aggaaccaag ccttctgccg ccgagccgga tttgacgttg
     5101 aaaggattga ggctgtcgaa tgtgccgaga acgccgagcg tcagcctgcc gcctttctgc
     5161 gcattggggt cggcataagg aaaatgcgta taatcggcgg gaagggccgg atcgccgtgc
     5221 atggcgatgg cgtatgccgg cgcggcgagc gcgggattgg cgaacgttat ggcggcggca
     5281 atcgccgcga gcactggcgc ggagcccacg cgcgaccggc gcggtgcata gaaaagcggc
     5341 ccccccaaca ggccgcgcgg acgaatcgtt tcgggacgca tttggctagt ctaag
//
LOCUS       DASWOG010000249         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_152276,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000249 DASWOG010000000
VERSION     DASWOG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152276"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..317
                     /gene="rpsJ"
                     /locus_tag="VGB93_05075"
     CDS             9..317
                     /gene="rpsJ"
                     /locus_tag="VGB93_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY0145516.1"
                     /translation="MNGQNIRIRLKAFDHRILDSSTKEIVSTAKRTGAQVRGPIPLPT
                     KIEKFTVNRSPHVDKKSREQFEIRTHKRVLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            450..1190
                     /gene="rplC"
                     /locus_tag="VGB93_05080"
     CDS             450..1190
                     /gene="rplC"
                     /locus_tag="VGB93_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0145517.1"
                     /translation="MRSGVIAQKLGMTRVFTDGGEHVPVTVLKLDGCQVVAQRTKEVN
                     GYTAVQLGIGHAKVKNVSKAERGRFAIAKVEPRLKLAEFPVEEATLLPVGAEITADHF
                     VVGQFVDVTGTSMGKGFAGPMKRWNFGGLRATHGVSISHRSHGSTGGRQDPGKTFKNK
                     KMAGHMGADRVTTLNLRVVQTDVERGLILVEGAVPGTKGGWIYVRDAVKKALPKDVPQ
                     PGKYRLATEAQGETPAEAPAAPAEGASS"
     gene            1187..>1604
                     /gene="rplD"
                     /locus_tag="VGB93_05085"
     CDS             1187..>1604
                     /gene="rplD"
                     /locus_tag="VGB93_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0145518.1"
                     /translation="MKIDITSLEGGSAGEIELNDAIFGLEPRADILARMVRYQLAKRR
                     AGTHQTQGRADVARTGKKMYKQKGTGNARHGSARVPQFRGGGRAFGPLTRSHAHDLPK
                     KVRALALKNALSAKAKDGGIIVWENAEFAEGKTKALL"
BASE COUNT          340 a          447 c          527 g          290 t
ORIGIN      
        1 aaaaaaagat gaacggccaa aacattcgca tccgcctcaa ggcgttcgat caccgaattc
       61 tcgacagctc gaccaaagag atcgtgtcga cggcgaagcg gacgggtgcg caggtgcgcg
      121 gcccgattcc actgccgacg aagatcgaga agttcacggt caaccgctcg ccgcatgtcg
      181 ataagaaatc gcgcgagcag ttcgagatca gaactcacaa gcgggtgctc gatatcgtcg
      241 acccgacgcc gcagacagtc gatgctttga tgaagctcga cctcgctgct ggtgtcgatg
      301 tggagatcaa gctttaagcg tcgctggcga gaagtgcaga cttctccgac tttggcgatg
      361 ccatagagat ctatgcactg tcttatgacg gtgcgatgaa aaggacgaac gggccggggc
      421 ggaagctttg gtcttgaagg acaaagaaca tgcggtcagg tgttatcgca cagaagctgg
      481 gcatgacccg cgtcttcaca gacggcggcg agcatgtgcc ggtcacggtt ctgaagctcg
      541 atggctgtca agtcgtcgcg cagcggacca aagaggtgaa cggctatacc gctgtgcagc
      601 tcggcatcgg tcatgccaag gtgaaaaacg tgtcgaaggc cgagcgcggc cggttcgcga
      661 tcgccaaggt cgagccgcgt ttgaagctgg ctgagttccc tgttgaggaa gcgacgctgt
      721 tgccagtcgg ggccgagatt acggctgatc atttcgtggt cggtcagttc gtcgatgtca
      781 ccggcacgag catgggtaag ggcttcgccg gtccgatgaa gcgctggaac ttcggcggtc
      841 tgcgcgcgac acacggtgtc tcgatctcgc accgctcgca tggttcgacc ggcggccgtc
      901 aggatccggg caagacgttc aagaacaaga aaatggccgg tcatatgggc gctgaccgtg
      961 tcacgacgtt gaatctgcgc gtcgtgcaga ccgatgttga gcgtggcctc atcctggtcg
     1021 agggcgctgt gcctggcacc aagggcggct ggatctatgt tcgcgatgcc gtgaagaagg
     1081 cattgccgaa ggacgttccg cagcccggta aatatcgttt ggccacggaa gcgcaaggcg
     1141 agacgcctgc agaggcgcct gcggcgccgg cggaaggagc ttcgtcgtga agatcgatat
     1201 cacctcgctc gaaggcggca gcgccggcga aatcgaattg aatgatgcca tttttggcct
     1261 ggaaccccgc gcggacattc ttgcccgcat ggtgcgctac cagcttgcca agcgccgggc
     1321 cggcacgcat cagacgcagg gccgcgccga cgtcgcgcgg accggtaaga aaatgtacaa
     1381 gcagaagggt accggcaatg ctcgccacgg ctcggcccgc gtgccgcagt tccgcggcgg
     1441 cggtcgtgcc ttcggtccgc tgacgcgttc gcacgcacat gatctgccga agaaggttcg
     1501 cgcgctggcg ttgaagaatg cgctctcggc caaggctaag gacggcggca tcatcgtttg
     1561 ggaaaacgct gaattcgccg aaggcaagac caaggcactg ctcg
//
LOCUS       DASWOG010000250         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_134351,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000250 DASWOG010000000
VERSION     DASWOG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134351"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1188)
                     /locus_tag="VGB93_05090"
     CDS             complement(106..1188)
                     /locus_tag="VGB93_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY0145519.1"
                     /translation="MADKPDRDSQTEEATDKKVRDAIERGNVPVSREVSIFAFVAGLL
                     VVMAFFLKTGSASLVLMLQQLLDNADGWHFSTGYDAVALCALIAQQAGALLVPAFIVF
                     IVAGLAASFSQNPPRIVFERIKPDLSRLSLAEGWHRMYSIRGATEFLKSVAKFIAIAI
                     IVTSMLQADRLQFVNALFVDPNALPDLISQMAMRFVSGIAVATLLLVAIDLIWSRRNW
                     RKELRMSRQEVKDEVKQGEGDPMLKARMRSLALDRRRRNMIAAVSRATIVVANPTHYA
                     IAIRYVREEGGAPLVLAKGKDFLALRIRAAAEENNIPVIEDKALARSMYENVEIDRMI
                     PPEFYRAVAELIHMLQRHARRRAPIH"
     gene            complement(1206..>2062)
                     /locus_tag="VGB93_05095"
     CDS             complement(1206..>2062)
                     /locus_tag="VGB93_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEY0145520.1"
                     /translation="PMAVIEDMIEEFAGNFARGAGLMISPNDAEHLLTGLLSPEEISD
                     IMSDVTGNSNQSIWHRLSGVPEALFAGYLVKEHPQTVAAILSKVTPVCAAKVMGHLPR
                     EFRNEVMRRMVGIAVVSEPAMRLIESTLQEDLLVNVARNNRNAHNGRMADIINKMERE
                     QMEDVLQSLAEARPKAAETLRGLLFTFDDIIKLPAKSRLALFDQIPTERVVLALKGTD
                     ATFRNSILSTLSGRARRVVETELSSGGPAPHREVVKARRMIADLALEMAERGELELNS
                     EEDDEEIY"
BASE COUNT          443 a          600 c          584 g          435 t
ORIGIN      
        1 ttcatcatcc gaagttcggc cgccacgtcc atgatcccct ctgcgatggc tttagcccgc
       61 aagagtgatt gatcaccgcg cgaatctgct agcattagcg tactttcaat gtatcggcgc
      121 ccggcggcgc gcgtgtctct gaagcatgtg gatcaattcc gccacggcgc gataaaattc
      181 aggcggtatc atccggtcaa tttccacatt ctcgtacatg gaccttgcga gagccttgtc
      241 ttcgataacc gggatattat tctcttctgc cgccgcacgg attcgcagag cgagaaaatc
      301 cttgcccttg gccaggacaa gcggcgcccc gccctcctct cgcacgtagc ggatcgcaat
      361 ggcatagtgc gtagggttgg cgacgacgat cgtcgcgcgg gaaacggcgg caatcatatt
      421 ccgccgccga cggtcgagcg cgagcgaacg catacgcgcc ttgagcatcg ggtcgccttc
      481 gccctgcttg acttcgtcct ttacctcctg ccggctcatg cggagttctt tgcgccaatt
      541 tcttcgcgac caaatgagat cgatcgcgac cagaagcagg gtcgcgacgg caatgccgga
      601 gacaaaccgc attgccattt gactgatgag atccggaaga gcattcggat cgacgaagag
      661 cgcgttgacg aattgcagcc tgtcggcctg cagcatcgac gtaacgatga tggcgatcgc
      721 gatgaatttc gccacgctct tgagaaattc ggtcgctccc ctgatgctgt acatgcggtg
      781 ccaaccctcc gccagcgaaa gccgcgaaag atctggtttg atgcgctcga acacaatgcg
      841 cggtggattc tgtgaaaagg atgcggcgag gcccgcgacg ataaagacaa taaatgcagg
      901 caccaacagc gcaccggcct gctgcgcaat gagcgcgcat aatgcaactg catcataccc
      961 tgtcgaaaaa tgccaaccat cggcattatc gagaagctgc tgcaacatga ggaccagaga
     1021 agctgaaccg gtcttcagga agaaggccat gaccacgagc agaccggcta cgaaggcaaa
     1081 aatcgagact tcacgcgaaa ccggaacatt gccgcgctcg atcgcgtcgc gaaccttttt
     1141 gtcggtggct tcttctgttt gactgtccct gtccggttta tccgccatgc gagaatctca
     1201 accggtcagt agatctcttc atcgtcttct tcggaattca gttcaagttc tccgcgctcg
     1261 gccatttcaa gcgcaagatc ggcgatcata cgccgcgctt tgacgacctc gcgatgcggc
     1321 gccggccctc cactggaaag ctcggtctcc acgacgcggc gcgcgcggcc cgaaagggtc
     1381 gacagaatcg aattgcggaa ggttgcgtcg gtgcccttga gtgcaaggac gacgcgttct
     1441 gtcggaatct gatcgaacag cgccaagcgg gatttcgccg gcagcttgat gatgtcatcg
     1501 aatgtgaaga gcaatccgcg caaggtttcg gcggctttcg gccgtgcttc ggcaaggctt
     1561 tgcaagacgt cttccatctg ctcgcgctcc atcttgttga tgatgtcggc catgcggccg
     1621 ttatgcgcgt tgcgattgtt gcgggcgacg ttgacgagaa gatcttcctg cagagtgctt
     1681 tcgataaggc gcatggccgg ctcggaaacg accgcgatgc cgaccatgcg ccgcatgact
     1741 tcgttgcgga actcgcgcgg caaatgtccc ataacttttg ccgcgcaaac tggcgtgact
     1801 tttgacagga tggcggcaac agtttgcgga tgttccttca ccagatagcc agcaaatagt
     1861 gcctccggga cgcccgaaag ccggtgccag atggactggt tggaattgcc cgtgacatcc
     1921 gacatgatgt cggaaatctc ttcgggcgaa agaagccccg tgagaagatg ctcggcgtca
     1981 ttcggcgaga tcatcaaccc ggcacctctg gcgaaattgc cggcgaattc ctcgatcata
     2041 tcctcgatga ccgccatcgg ga
//
LOCUS       DASWOG010000251         6095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_335620,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000251 DASWOG010000000
VERSION     DASWOG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335620"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /gene="cutA"
                     /locus_tag="VGB93_05100"
     CDS             complement(<1..276)
                     /gene="cutA"
                     /locus_tag="VGB93_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY0145521.1"
                     /translation="MSGEVAKDYAIVLTTCSAEVAPRIAEALVGAKLAGCVQRLPIES
                     TYRWKGEVETAWEVLLLIKIRAADYAAVKQAILALQEYETPEIIMLPV"
     gene            complement(273..1289)
                     /locus_tag="VGB93_05105"
     CDS             complement(273..1289)
                     /locus_tag="VGB93_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0145522.1"
                     /translation="MTERKKVNLALQGGGSHGAFTWGVLDYLLEDDRLDIAAVSGTSA
                     GAMNAVVMAEGWLENGRDGARENLRRFWRSISTENALSPFQAKFFDLFLGPQTLGGQF
                     SALWGDLLLHFASPYQFNPFNVNPLRKYLVDTIDFEKVRAFDGFKIFIAATNVRTGKI
                     RVFKGAELTADHVMASATLPTLFQTIVIDGEPYWDGGYMGNPALFPFFYASSTPDIVI
                     IQVNPLERDTIPHSARDIQNRLDEISFNGALMGELRAIDFVVRLIDAGKLPLGEYTRP
                     LVHRIDGGDLLRPFSAATKSDASWQLINSFFEFGRTCGKQWLDETYDRVGKEGTLDLR
                     MAYS"
     gene            complement(1316..2101)
                     /locus_tag="VGB93_05110"
     CDS             complement(1316..2101)
                     /locus_tag="VGB93_05110"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY0145523.1"
                     /translation="MTLKTRNAIVTGSTSGIGLAIARGLAAEGANVVINGFGDSGAIE
                     KERSGIEKDFGVKAIYSSADGTKPEEVRGMVKTAENDLGSVDILVNNAGIQHVDAIEN
                     FPIELWDKIIALNLSAAFHAIAAAIPGMKSRKWGRIISTASAHSKVASPFKSAYVSAK
                     HGIDGLTKTAALELATFGVTVNCISPGYVWTPLVEKQIPDTMKARNLTREQVINDVLL
                     TAQPTKEFVTVDQVAALALFLCSDAASQITGANISIDGGWTAE"
     gene            2234..2740
                     /locus_tag="VGB93_05115"
     CDS             2234..2740
                     /locus_tag="VGB93_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0145524.1"
                     /translation="MTASRVPRPRPKKALTVAALRSALNNAEDLCRREGTKLTPGRRR
                     VLEILAEEGRPLGAYDLIEKIATTTGKHPAPISIYRALDFLLENGLVHRLASRNAFLA
                     CAHGHRHEEPVVFLICETCGSVTEATSKALRREIAALGAESGFTPHSQVLELTGLCGA
                     CEGKGSTA"
     gene            2878..4173
                     /gene="ispG"
                     /locus_tag="VGB93_05120"
                     /gene_synonym="gcpE"
     CDS             2878..4173
                     /gene="ispG"
                     /locus_tag="VGB93_05120"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0145525.1"
                     /translation="MSETLTRLIDPANVLASGPAPHRRSVGVRVGSGKGAIMVGGGAP
                     VIVQSMTNTDTADIEGTTKQVADLARAGSELVRITVDRDEAAAAVPHIRERLDRMGVG
                     VPLIGDFHYIGHKLLADHPACAQALAKYRINPGNVGFKDKRDTQFSAMIECAIENGKA
                     VRIGANWGSLDQELLTRLMDENANSPKPLDASAVTREAMVQSALLSAARAEELGLGRD
                     RIILSAKVSNVQHLITVYQMVAARSDYAIHLGLTEAGMGSKGIVASSAALGVLLQQGI
                     GDTIRISLTPEPGGDRTVEVKVGQEILQTMGFRTFVPLVAACPGCGRTTSTVFQELAR
                     DIQAYIRDEMPNWKTRYPGVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPAAPV
                     FIDGKKSVTLRGDGIAAEFKVMVQDYIERRFGGAKDAAE"
     gene            4370..4876
                     /locus_tag="VGB93_05125"
     CDS             4370..4876
                     /locus_tag="VGB93_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145526.1"
                     /translation="MRKGWASYIFAGALAFAFGSTGAEAASKHHHHAQTHYSHYQNVG
                     GMSVGVMEPGKVYSKYGHYTELAGDPHSGLGFYPLPYQYRVGAWRYHMRHAPPIPPVI
                     QAARDQAVESYSWEMPTPDDAYAYGVYDPVDGVGSPFFAGYYGPAGEDDDDDSDSGFP
                     FGHSYAPR"
     gene            complement(4873..5508)
                     /locus_tag="VGB93_05130"
     CDS             complement(4873..5508)
                     /locus_tag="VGB93_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0145527.1"
                     /translation="MFVCLLFGVLVGVAMPSDLRHVTRVLIGWNAGMFAYFVAVGVMV
                     WSSDNRSLARRAAAQDDGRFAILFLTIFAAAAAFGAIFYQLLLVKDEHGLNRSFHVGL
                     AALTVISSWAFIHIMFALHYASECFLVRNTKVSPEAKVHGGLRFPGEDEPPDYLDFFY
                     FSFVIGVACATADVNITSRSIRRTALIHSVVAFFFNSAVLALTVNIAAGLV"
     gene            complement(5577..>6095)
                     /locus_tag="VGB93_05135"
     CDS             complement(5577..>6095)
                     /locus_tag="VGB93_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0145528.1"
                     /translation="ADPATDADPAIRAALVLALARAAGIGNMVGGQVMDLEAELSQAP
                     HSPEHVIQVQAMKTGALLHYSVEAGAIFARADEKTRTALSRYGKALGAAFQVADDILD
                     VEADEAALGKRAGKDADRNKATLVAAVGLDAARKRRDALAQEAISALDVFPAERAAVL
                     KEAARFTVARKN"
BASE COUNT         1230 a         1903 c         1816 g         1146 t
ORIGIN      
        1 gaccggcagc atgatgatct ccggcgtctc atattcctgc agtgcgagga tcgcctgctt
       61 caccgcggca taatccgcag cgcgaatttt aatcagcagc agaacttccc acgccgtctc
      121 gacctcaccc ttccagcgat aggtgctttc gatcggcagc cgctgcacgc agccggcgag
      181 tttggcgccg accagcgcct cggcgatgcg cggtgcgacc tctgcgctgc aggtggtcag
      241 gacgatcgcg tagtccttgg ccacctcgcc gctcacgaat aggccatgcg caggtccagc
      301 gtaccttcct tgccgacacg gtcataggtt tcgtcgagcc attgtttgcc gcacgtgcgc
      361 ccgaactcga aaaagctatt gatgagctgc cagctcgcgt cggatttggt tgcagcgctg
      421 aagggccgca acagatcgcc accatcgatg cgatgaacca gtggccgcgt atattcgccc
      481 agcgggagtt taccggcatc gatcaaacga acgacgaaat cgatggcgcg caattcgccc
      541 atcagcgccc cgttgaaact gatttcatcg aggcgatttt gaatgtcgcg agccgaatgc
      601 ggaatcgtgt cgcgctcaag cggattgacc tggatgatga cgatatcggg tgtgctcgac
      661 gcatagaaaa atggaaacag cgccggattg cccatgtatc cgccgtccca ataaggctca
      721 ccgtcgatga cgatggtttg aaacagggtc ggcaatgtcg ccgaagccat gacgtgatcc
      781 gccgtcagct ctgcgccctt gaaaaccctg atcttgccgg tgcgcacatt ggtggccgcg
      841 atgaaaattt tgaacccgtc gaaagcacga accttctcga aatcgattgt gtcgacgaga
      901 tatttgcgca gcggatttac attgaatgga ttgaattgat aggggctggc aaaatgcagc
      961 agcaaatcgc cccatagagc ggagaattgg ccgcccaatg tctgcggccc taaaaacaga
     1021 tcgaaaaact tcgcctgaaa tggcgagagc gcgttctccg ttgaaatcga gcgccagaat
     1081 cgcctgagat tctcgcgcgc gccgtcacgg ccgttttcga gccagccttc ggccatgacg
     1141 acggcattca tcgcgccggc gctggtgccg ctgacggcgg cgatatcaag gcgatcgtcc
     1201 tcgagcagat agtcgagaac gccccatgtg aatgcgccgt gcgagccgcc gccttgcagc
     1261 gcgagattga ctttcttgcg ttcggtcata tgcgacggct ctccttatgt tgcgattatt
     1321 cggccgtcca gccgccgtcg atcgagatat tggcgccggt gatctggctt gccgcgtccg
     1381 aacacaggaa gagggcgagc gccgcgacct gatcgacggt gacaaattct ttcgtcggct
     1441 gtgcggtgag cagaacgtcg ttgatgactt gttcgcgcgt cagattgcgc gccttcatcg
     1501 tatccgggat ctgcttctcg acaagcggcg tccagacata gccgggcgag atgcaattga
     1561 ccgtgacgcc gaacgtcgcg agttccagcg ccgccgtctt cgtcaaaccg tcgataccat
     1621 gtttcgccga cacgtaagcc gacttgaacg gcgaggcgac tttcgaatgc gccgaggcgg
     1681 tggagatgat gcggccccat ttgcgtgatt tcatgccggg gatcgccgcg gcaatcgcat
     1741 gaaacgccgc cgacagattg agcgcgatga tcttgtccca gagttcgatt ggaaaatttt
     1801 cgatcgcatc gacgtgctga atgccggcat tgttgacgag aatatcgacg ctgccgagat
     1861 cgttttccgc cgtcttcacc atgccgcgga cttcttctgg cttggtgccg tcggcggacg
     1921 agtagatcgc tttgacgccg aaatcctttt caatgccaga gcgctccttc tcgatcgcac
     1981 cggaatcgcc aaatccgtta atgaccacat tggccccttc ggccgcgagg ccgcgggcga
     2041 tggcaaggcc gattccgctt gttgagccgg tcacgatggc attgcgtgtc ttgagcgtca
     2101 tgcataactc catgaggtct tgaacgtgcc ccgccggcac gcagcgatct cagccgaaac
     2161 attaggctcg cgcaatgcgg gctgaagggt ggcggctgtc gcctttgtga tcgcagttgg
     2221 atataatgaa atcatgactg catcccgcgt gccgcggccg cgccccaaaa aagctctcac
     2281 tgtcgctgcg ctccgtagcg cgttgaacaa tgccgaggat ctttgccgcc gggaaggcac
     2341 caagctgacc cccggacgcc ggcgtgtgct ggaaattctc gcagaagaag gccgaccgct
     2401 gggtgcctac gacctgatcg aaaagatcgc caccacgaca ggcaagcatc cggcgccgat
     2461 ctcgatctat cgcgccctag acttcctgct cgaaaacggc ctcgtgcatc gccttgcctc
     2521 ccgcaatgcc tttctcgcct gtgcgcacgg acatcggcac gaggagccgg tggttttcct
     2581 gatctgcgag acttgcggct cggtgacgga agcgacatct aaggccttgc gccgtgaaat
     2641 tgccgcgctc ggcgctgaat ccggcttcac gccgcatagt caagttcttg aattgacagg
     2701 actttgcgga gcgtgcgaag gaaagggcag cactgcttga attataagac gccgcgcgcc
     2761 gccgcttgac ggctgcggca attttgagca ccactccggc atgtgcctgt catgacgggg
     2821 tcatgctgag ggacaggcta gggcttgggc ccgcggtcag ccaatgagaa ggttttcatg
     2881 tccgagacac tgacacgctt gatcgatccc gccaacgtgc tggccagcgg gccggcgccg
     2941 catcgtcgct cggtcggcgt gagggtcggc tcgggcaaag gcgcaatcat ggtcggcggc
     3001 ggcgccccgg tgatcgtgca gtcgatgacc aatacggaca cggccgatat cgagggaacg
     3061 acgaaacagg tcgcagacct cgcccgcgcc ggctccgagc tggtccgcat caccgtcgat
     3121 cgcgacgaag ccgcggccgc cgtgccgcat attcgcgagc ggctcgaccg catgggcgtc
     3181 ggtgtcccgc tcatcggcga ttttcattat atcggccaca aactgctcgc cgatcatccg
     3241 gcgtgcgcgc aggcgcttgc caaatatcgt atcaatcccg gcaatgtcgg cttcaaggac
     3301 aagcgcgaca cccaattctc cgcgatgatc gaatgtgcca tcgagaacgg caaagcggtt
     3361 cgcatcggcg ccaattgggg ctcgctcgat caggaacttc tgacccggtt gatggacgag
     3421 aacgccaatt ctccgaagcc gctcgatgcc agcgccgtga cgcgcgaagc catggtccag
     3481 tcggcgctgc tctcggccgc ccgagcagaa gaactcggcc tcggccgtga ccgcatcatt
     3541 ctctcggcca aggtctccaa cgttcagcat ctgatcaccg tctatcagat ggtcgccgcg
     3601 cgcagcgatt acgcgatcca tctcggcctc accgaagccg gcatgggctc gaaaggcatc
     3661 gtcgcctcgt ccgccgctct cggcgtcctg ctccagcagg gcattggcga cacgatccgc
     3721 atctcgctga cgcctgagcc cggcggcgac cgcaccgtcg aagttaaggt cggtcaggaa
     3781 atcctgcaaa cgatgggctt ccgcaccttc gtgccgctcg tcgctgcctg ccccggctgc
     3841 ggccgcacga cctcgaccgt gttccaggaa ctcgcccgcg acatccaggc ctacatccgt
     3901 gacgaaatgc cgaactggaa gacgcgctat cccggcgtcg agacgctcaa cgtcgcggtg
     3961 atgggctgta tcgtcaacgg acccggcgaa tccaagcacg ctgatatcgg catttccctg
     4021 cccggcaccg gcgagacgcc ggccgcgccc gtcttcatcg acggcaagaa gtccgtaacc
     4081 ctgcgtggcg acggcatcgc cgccgaattc aaggtcatgg tgcaggatta tatcgagcgc
     4141 cgcttcggcg gggccaaaga cgcggcggaa taggccgcgg ccgcacttcg ttaacgcggc
     4201 cgcacttcgt taaaatgtga acgaagtctt ttgcgcaacc tttgcgcaag tgacatcgtt
     4261 ttgatcccta tcagggcgga aactcggcat cggaagacta tattaaaggt cggcggtgag
     4321 agttgtcgcg ggaagcgacg ggattcaacc gccgcatgga gattgaccga tgcgcaaggg
     4381 ctgggcttct tacattttcg cgggcgcact cgcgttcgcc ttcggctcga ccggcgctga
     4441 ggctgcgagc aaacatcatc atcatgcaca gacgcattac agccactatc agaacgtcgg
     4501 cggcatgtcg gtcggcgtga tggagcccgg caaggtctat tccaaatacg ggcactacac
     4561 cgaactcgcg ggcgacccgc atagcggcct cggcttttac ccgctgccct accaatatcg
     4621 cgtcggcgcc tggcgttatc atatgcgcca cgccccaccg atcccgccgg tgatccaggc
     4681 ggcgcgcgac caagcggtcg aaagctacag ctgggaaatg ccgacgcctg acgacgccta
     4741 tgcctatggc gtctatgatc ccgtcgatgg cgtcggctcg ccgttctttg ctggctatta
     4801 cgggccggcc ggcgaagatg atgacgacga cagcgattca ggcttcccgt tcggtcattc
     4861 ctacgcgccg cgctagacca agcccgctgc gatattgacc gtcagcgcca gcacggcgct
     4921 gttgaagaaa aaggccacga cgctgtgaat aagcgccgtg cgccggatcg agcgtgacgt
     4981 gatgttgacg tccgccgtgg cgcaggcgac gccgatgacg aaagagaaat aaaagaagtc
     5041 gagataatcc ggcggctcgt cctcgccggg gaagcgcagg ccaccatgga ctttggcctc
     5101 gggcgacact ttcgtattgc gcaccagaaa acactcgctc gcgtaatgca gcgcgaacat
     5161 gatatggatg aacgcccagg agctgatgac ggtcagcgcg gcaagcccga catggaacga
     5221 acgattcaga ccgtgctcgt ccttcaccag caataattga tagaagattg cgccgaaggc
     5281 cgccgccgcc gcgaagatcg tcaggaaaag aatagcaaac cggccatcgt cctgcgcggc
     5341 cgcacgacgc gccaaagaac gattgtcgct cgaccaaacc atcacgccaa ccgcgacaaa
     5401 ataggcgaac attccggcat tccagccgat gagtacgcgt gtcacgtgcc gcaagtcgct
     5461 cggcatcgcc acaccgacaa gcacgccaaa cagcaggcaa acgaacaagc gcggtcgcga
     5521 ccggatcacg cggaccggcc agccgaaacg ccggcgatgc gagagacctg gcccggtcaa
     5581 tttttacgcg cgacggtgaa acgcgccgct tctttcaaaa ccgcggctcg ttccgcgggg
     5641 aagacatcga gggcgctgat ggcctcctgc gccagagcat cgcgacgctt gcgcgctgcg
     5701 tcgagcccga ccgcagcaac aagcgtcgcc ttgttgcgat cggcgtcctt gccggcgcgt
     5761 ttgccgagcg ccgcttcgtc ggcctcgaca tccagaatat cgtcggcaac ctgaaaggcc
     5821 gcgcccagcg ccttgccata acgtgatagc gccgttcgtg tcttttcgtc ggcgcgggcg
     5881 aagatcgcac cagcttcgac cgaataatgc aacagtgccc ccgtcttcat cgcctgaacc
     5941 tgaatgacat gttccggcga gtgcggcgct tgtgaaagct cagcttcgag gtccattacc
     6001 tgaccgccga ccatgttgcc gatgcctgcg gcacgcgcga gcgccaggac cagcgcggcg
     6061 cggatcgcgg ggtctgcatc cgtcgcggga tccgc
//
LOCUS       DASWOG010000252         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_115917,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000252 DASWOG010000000
VERSION     DASWOG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115917"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="sucD"
                     /locus_tag="VGB93_05140"
     CDS             complement(<1..769)
                     /gene="sucD"
                     /locus_tag="VGB93_05140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0145529.1"
                     /translation="MSILIDEKTRIIVQGLTSEKATFHAKDMIAAGSNIVGGVVPGKG
                     GRTHIGLPVFNTVKDAVREVGAEASITFVAPSFAADAIMECADAGIRLVCSITDGIPA
                     QDMMRVKRYLMRYPKDRRTMMVGPNCAGIISPGKAMLGIMPPSIYVRGTVGVISRSGT
                     LGYEAATQMKALGIGITTSVGIGGDPINGSSFMDHLALFNEDDETEAVLMIGEIGGPQ
                     EAEAAAWVKDNMMKPVVGFVAGLTAPKGSRMGHAGAII"
     gene            complement(780..1970)
                     /locus_tag="VGB93_05145"
     CDS             complement(780..1970)
                     /locus_tag="VGB93_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HEY0145530.1"
                     /translation="MDIHEYQAKQLLAQFGVAVAPGAVAFTPEQAVYVATELGGSRWA
                     VKAQIHSGARGKAGGIKLCSHYKEVRQAAHDLLGKRLVTIQNAPDGQPVQRVYIEVAA
                     AYEKEFYLGFVLDRKTERVRVIASQHGGMEIEEIVKTDPNAVIQIVVEPAVGLQAFQA
                     REIAFALGLNIKQVARASAVILASYRAFRDCDALMLEINPLVLTADDKIIALDAKMSF
                     DDNALFRRPNIAAMHDASQQDPREAQAAEHNLNYIGLDGEIGCIVNGAGLAMATMDMI
                     KHAGGSPANFLDVGGGASAERVATAFRLVLSDENVKSVLVNIFAGINRCDWVAEGVVQ
                     AVKETEIKVPLIVRLAGTNVEAGRKIIKESGIPIISAETLGEAANKAVAACRDHKASL
                     AAVQ"
     gene            2316..3026
                     /locus_tag="VGB93_05150"
     CDS             2316..3026
                     /locus_tag="VGB93_05150"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0145531.1"
                     /translation="MDERSARDDSALLPARLVEGYEMFLRGRFRREQDRYRDLADRGQ
                     SPNVLLIGCCDSRVSPEVIFDADPGEIFVLRNIANLIPPYASDCERHGTSAALEYAVK
                     VLKVGHIVIMGHAMCGGVRAYVETQSGFDVKLAPGDFIGQWISLLGEAAAKLGPRTEP
                     AADYAERLGRAAIVESIANLRTFPDVRAAEAAGELVLHGAYFGVSDGKLFGLDEATGK
                     FELLAANAHAAALGTPRF"
     gene            3036..>3224
                     /locus_tag="VGB93_05155"
     CDS             3036..>3224
                     /locus_tag="VGB93_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HEY0145532.1"
                     /translation="MPATFDLQRFVAAHAPVYAQVRAELRNGHKTTHWMWFIFPQVAG
                     LGRSPMAVRFAIGSTAEAR"
BASE COUNT          570 a          981 c         1011 g          662 t
ORIGIN      
        1 atataatcgc gccggcatgg cccattcggc tgcccttcgg cgccgtgagg ccggcgacga
       61 aaccaaccac gggcttcatc atattgtcct tcacccaggc tgcggcctcg gcttcctgcg
      121 ggccgccgat ttcgccgatc atcaacacgg cttccgtctc gtcgtcctcg ttgaagagcg
      181 cgaggtgatc catgaacgaa ctgccgttga tcgggtcgcc gccaatgccg acgctggtgg
      241 tgatgccgat gccgagcgcc ttcatctgcg tcgcagcttc atagccaagc gtgcccgagc
      301 gcgagatgac gccgaccgtg ccgcgcacat agatgctcgg cggcatgata ccgagcatgg
      361 ccttgccggg gctgatgatg ccggcgcagt tcggcccgac catcatcgtg cgcctgtcct
      421 tgggatagcg catcagatag cgcttcacgc gcatcatgtc ctgtgccggg atgccgtcgg
      481 tgatcgagca gacgagccgg atacccgcat ccgcgcattc catgatggcg tcagcggcaa
      541 aagacggcgc aacgaaggtg atcgaggctt cagcgccgac ttccctcacc gcgtccttca
      601 cggtgttgaa gaccggcagg ccgatatggg tgcggccgcc tttgccgggc accacgccgc
      661 cgacgatgtt gctgccggcc gcgatcatgt ctttcgcgtg gaaggtcgcc ttctcgctgg
      721 tcaggccttg tacgataatt cgggttttct cgtcgatcag aatgctcatg gtgcgtccct
      781 cattgtacgg cggcgagcga ggctttgtgg tcgcggcagg cggcgaccgc cttgtttgcc
      841 gcttccccca aagtttcggc actgatgatc ggaatgccgc tttctttgat gatcttgcgg
      901 ccggcttcga cattggtgcc ggcgaggcgg acgataagcg gaaccttgat ctccgtctcc
      961 ttcaccgcct gaacgacgcc ttcggcaacc cagtcgcaac ggttgatgcc ggcgaaaatg
     1021 ttgacgagca cgctcttcac gttttcgtcc gagaggacga gacggaaggc ggtcgcgacg
     1081 cgctcggccg aggcgccgcc gccgacgtcg aggaagttcg ccgggctgcc gccggcatgt
     1141 ttgatcatgt ccatcgtcgc catggccagg ccggcgccgt tgacgataca accgatctcg
     1201 ccgtcgaggc cgatgtaatt gagattgtgc tccgccgcct gcgcttcgcg cggatcctgc
     1261 tgcgaggcgt cgtgcatcgc cgcgatattg ggccggcgga agagcgcgtt gtcgtcgaac
     1321 gacatttttg cgtcgagcgc gatgatcttg tcgtcggcgg tcagcaccag cgggttgatt
     1381 tcgagcatca gcgcatcgca atcgcggaag gcgcgatagg aggcgaggat cacggcggat
     1441 gcgcgcgcga cctgcttgat gttcagcccg agtgcgaagg caatttcgcg cgcctggaaa
     1501 gcctgaagac cgacggcagg ctcgacgacg atctggatca ccgcgttggg atcggtcttg
     1561 acgatctctt cgatctccat gccgccatgc tgtgaggcga tgacgcgcac acgctcggtc
     1621 ttacgatcaa gcacgaagcc gaggtagaat tccttctcgt acgccgccgc gacttcgata
     1681 tagacgcgct gaaccggctg gccgtccggc gcgttctgga tcgtgacgag ccgcttgccg
     1741 aggagatcat gcgccgcctg gcgcacttcc ttatagtgcg agcagagctt gatgccgccc
     1801 gctttgccgc gcgcgccgga atgaatctgc gcttttaccg cccagcgcga cccaccaagt
     1861 tcagttgcaa cataaaccgc ttgctctggc gtaaaggcca ccgcacctgg cgcaacggcg
     1921 acgccgaatt gcgccagcaa ttgcttggcc tgatactcat gaatatccat taccggcaat
     1981 ctcctccgag aaaggcgtcg tctaaaaggt tggttagccg agtttattcg gcttcgccag
     2041 atagcaaagc ggacgagatg gccgaagcct ttgtctccga gcgagttttt cgcgttgcgg
     2101 aatcgctgcg gtgacttatg gtcggcggct catgcgtgcg gtttacgaga caaaaccggt
     2161 caagagcgct tcggcgaagt gcggcggcgg tccggaaaac tgcgctaacg tgccgcaatt
     2221 ttattgcagt gccataaacg gctcagcttg caggttgtcg cgcgattttg caaaagctga
     2281 aacaaaaagt tcatcgattt aacgggaggt ttggcatgga cgaacgcagc gctcgggatg
     2341 atagcgcgct tttgcccgcc cggcttgtcg aaggctacga gatgtttctg cgtggtcgct
     2401 tccgtcgcga gcaggatcgc tatcgcgatc tcgctgaccg cggccagagc ccgaacgtcc
     2461 tgctcatcgg ctgctgcgat tcccgcgtct caccggaggt gatcttcgat gccgacccgg
     2521 gcgaaatctt cgttctgcgc aacattgcca atttgatccc gccttacgcg tctgactgcg
     2581 aacgccacgg cacatcggca gcgctggaat atgcggtcaa ggttttaaaa gtcgggcaca
     2641 ttgtcatcat gggccatgcg atgtgcggcg gcgtgcgcgc ctatgtcgaa acgcaatccg
     2701 gcttcgatgt gaagctggcg ccgggcgatt tcatcggcca gtggatttcc ctgctgggcg
     2761 aggctgcagc caaactcggc ccgcggacgg agccagcggc agactatgcc gaacggctcg
     2821 gccgcgccgc gatcgtcgaa tcgatcgcca atctgcgcac attcccggat gtccgtgccg
     2881 cggaagcggc gggcgaactg gtcttgcacg gcgcctattt cggcgtctcc gatggcaagt
     2941 tgttcggcct cgacgaagcg acaggcaagt tcgagctgct cgcggcaaac gctcatgcgg
     3001 cggcgcttgg cacgccacgg ttttgacgcg cggcgatgcc ggcgactttc gatctgcaac
     3061 gcttcgtcgc ggcacacgcg cccgtttatg cgcaggtccg cgcggaatta cgaaatggcc
     3121 ataaaacgac gcattggatg tggttcattt tcccgcaggt cgcgggtctt ggccggagcc
     3181 cgatggcggt tcgcttcgcg atcggttcga ccgcggaagc gcgt
//
LOCUS       DASWOG010000253         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_280812,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000253 DASWOG010000000
VERSION     DASWOG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280812"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /gene="mnmG"
                     /locus_tag="VGB93_05160"
     CDS             <1..1379
                     /gene="mnmG"
                     /locus_tag="VGB93_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY0145533.1"
                     /translation="RVPAGRVGEPPAQQLSASLLAHGFALGRLKTGTPPRLDGTTIDW
                     AMLERQPGDEEPEPFSFLTSAITSEQVDCFITRTTEAGHDLIRANIGHSPVYSGAIAG
                     RGPRYCPSIEDKVMRFGDREAHQIFLEPEGRDDPTIYPNGISTALPATLQDAFLRTIP
                     GLANVRMLQPGYAIEYDFIDPRELTPSLETKRVAGLFLAGQINGTTGYEEAGAQGLVA
                     GLNAAALAGGTAPITFDRSEAYLGVMIDDLVTRGVTEPYRMFTSRAEYRLSLRAENAD
                     QRLTGKGIRLGAVGQERAIAFESKQAQLEAARTQLMALSLTPNEAARHGLKINQDGIR
                     RTAFDLLGFPVVEMAVLGRIWPELAAIAPKIAAQIEIDAKYAVYLERQAADIASFRRD
                     EALRIPDTLDYRAIAGLSAELRDKLISIRPQTVGQAGRIEGITPAALTLLAAHSKRRL
                     NESRAIGK"
     gene            1369..2028
                     /gene="rsmG"
                     /locus_tag="VGB93_05165"
     CDS             1369..2028
                     /gene="rsmG"
                     /locus_tag="VGB93_05165"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY0145534.1"
                     /translation="MASDFESDRDAARALFPLPPETVRRLDIYADLLRKWQKTINLVA
                     NSTLPQLWTRHIADSLQVSAAVPEARIWADLGSGGGFPGLVTAIRLAETPGARVHLIE
                     SDQRKCAFLRDVSRETSAPAIVHNARIETIIDNLGDPIDAVSARALAPLPVLIDYTKN
                     LLAKGAVGVFLKGQDYEAEVKALSAAGQYRIMSASSVTAPVSRLLLVFDKEAETQARS
                     G"
BASE COUNT          408 a          660 c          663 g          419 t
ORIGIN      
        1 agcgagttcc tgcgggacgc gttggcgaac cgccggctca gcagctttcc gcgagtcttt
       61 tggcgcatgg gtttgcgctc ggacgtctga agaccgggac gccgccgcgt ctcgacggca
      121 cgaccatcga ttgggcgatg ctcgaacgcc agccgggcga cgaagagccg gagccgtttt
      181 ccttcctcac gtcggcgatc acgagcgagc aggtcgattg cttcatcact cggacaaccg
      241 aggcgggaca tgacctaatc cgcgccaata tcggccattc gccggtctat tccggcgcca
      301 ttgccggtcg cggcccgcgc tattgccctt cgatcgaaga taaggtgatg cggttcggcg
      361 atcgcgaggc gcaccagatc tttctggagc cggaggggcg cgacgatccg acgatttatc
      421 cgaatggcat ttcgacagcc ctgcccgcga cgttgcagga cgcgtttctg cgcacgatcc
      481 ctggcctcgc caatgtgcgg atgctgcagc ccggctacgc gatcgaatat gatttcatcg
      541 atccacgcga actgacgcca agcctggaaa caaagcgcgt cgcagggctg ttcctcgccg
      601 gccagatcaa tggcacgacg ggctatgagg aggccggcgc acaaggcctt gtcgccggct
      661 tgaacgcggc cgcgctggcg ggcggcactg cgccgatcac cttcgatcgg tctgaggctt
      721 accttggcgt gatgatcgac gacctcgtga cgcgcggcgt cacggagccc tatcgcatgt
      781 ttacctcacg cgccgaatat cggcttagcc tgcgggccga aaatgccgac cagcggctga
      841 ccggcaaggg catccgtctc ggcgcggtag ggcaagaacg ggcgatcgcc tttgagagca
      901 agcaggcgca actcgaggcg gcgcggacac agcttatggc gctgagcctc acgccaaacg
      961 aagccgcgcg gcatgggctc aagatcaatc aggatggcat ccgccgcacc gcgttcgacc
     1021 tgcttggctt tcccgttgtc gaaatggcgg ttctcgggcg catctggccg gaacttgcgg
     1081 cgatcgcgcc gaaaatcgca gcgcagatcg agatcgacgc caaatatgcc gtctatctgg
     1141 agcgccaagc cgccgatatc gcaagtttcc gacgcgatga ggcgctgcga attccggata
     1201 cgctcgacta tcgggcgatt gcgggtctct cggccgagct tcgcgacaag ctcatttcta
     1261 ttcgtccgca gaccgtgggc caggccggcc ggattgaagg catcaccccg gcggcgctga
     1321 cactgctcgc ggcccattcc aagcgccgtc tcaacgagag ccgcgcgatt ggcaagtgat
     1381 ttcgaaagtg atcgcgacgc ggcgcgggcg ctttttccat tgccgccaga gaccgtccgt
     1441 cgcctcgata tctacgctga tctgctgcgc aaatggcaga aaacgatcaa tctcgtcgct
     1501 aattcgactc tgcctcaact ctggacccgg catatcgcgg attccttaca agtctctgcg
     1561 gccgtgccgg aggcacgtat ctgggccgac cttggctcgg ggggcggctt ccctggcctt
     1621 gtgaccgcta tccggctcgc tgagacgccc ggcgcgcggg ttcatctgat cgaatcggat
     1681 cagcgcaaat gtgctttcct gcgcgatgtt tcacgtgaaa catcggcgcc cgcgattgtt
     1741 cataacgcgc gcatcgaaac aatcatcgac aatctgggtg acccgatcga tgccgtaagc
     1801 gcccgcgctc tggcgccttt gcctgtcttg atcgattaca cgaaaaatct gctcgccaaa
     1861 ggcgctgttg gcgtttttct caagggacaa gactacgaag cggaggtcaa agccttgtcc
     1921 gcggcgggcc aataccgcat catgtcggca tcaagcgtaa cagcgccggt gagtcggttg
     1981 ctccttgtgt tcgacaaaga ggccgaaaca caggcgcgga gcggatgagt ttgcggcgtg
     2041 acgcgcgaaa agtctccaaa aacaatcatt tagagatatc gcatgggtgc gcaggtagtt
     2101 ggattacgga gacggcgtgt ggcttattct cccgatcttc gcgttctggt
//
LOCUS       DASWOG010000254         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_170834,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000254 DASWOG010000000
VERSION     DASWOG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170834"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VGB93_05170"
     CDS             <1..945
                     /locus_tag="VGB93_05170"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0145535.1"
                     /translation="ICHSDLHQARNQWNNAVYPMDPGNEIVGRVVKVGAHVKKLQVGD
                     FAGVGCMVDSCRQCSACNDDLENYCEHKPTYTYNDRERGGDRLTFGGYSEQIVVEERF
                     VVKVPASLDLKAVAPLLCAGITTYSPLRHWKVGPGDKVGVIGLGGLGHMGIKFAKALG
                     AHVVMITTSPGKAQDAKKLGADEVLISTDAAAMQAHAASFDFLLNTVPVPHDLNPYIA
                     LLKRDRAMVLVGVLTELDPPVQGGGLIMGRKSVAGSAIGGMKETQEMLDFCGEHNIVA
                     DIEMVDIQTVNEAYERLIKNDVKYRFVIDMDSLRKEAA"
     gene            complement(1044..2186)
                     /locus_tag="VGB93_05175"
     CDS             complement(1044..2186)
                     /locus_tag="VGB93_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0145536.1"
                     /translation="MTLPNNSISADRRDVICSGGAFAFSSFISLLFSTSETVQAAALQ
                     NKPPEIDQLSVRIVTDSYQMAVAPNIKVGNLEIKRFGFAVGDHPPEKAILSEFGLSLH
                     ATSLIGGDSRRVLIDFGYTPQTLLNNFDLLGLDPGSIDALVLSHGHYDHFGGLVGFLD
                     HSKGKLKPKLPIFLGGEECFCARQWVAPPMKGDFGALNRDAIEKANLSIMFAPGPAVV
                     ADHGFTTGRIELTSFEKVLSPSRMKIGRTGSFGCYPEDFTQDERNKGIIPDQFRHEIA
                     TAFNLKGRGLIILTSCSHRGLVNTIKQTQSASGVEKIHAIIGGFHLAPQKEDYIRETI
                     KELAAVNPDYVIPLHCSGELFYDIMKAELPTKLIRGYTGSEIIFRA"
     gene            2454..3395
                     /locus_tag="VGB93_05180"
     CDS             2454..3395
                     /locus_tag="VGB93_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0145537.1"
                     /translation="MAPKTVLIIAPPRVTLQDVTGPWEVFCRAEGLRPGTYDVAVVSA
                     GLQKKVETKFGLGIVCERSVRDFAGPLDTVLVAGSDEGVSGEADPAFLDWLRDAATRT
                     RRMGSICTGSFYLAHAGLLAGRHATSHWRYLDRLADSFPDVSVERDPIFVRDGDIYTS
                     AGITAGIDLALALVEADCGHDVSQAVARDLVVFLQRQGDQPQLSTALALRMADRDPIR
                     SLQQWMPDNLQSVVRVEDMAAYVHMSPRNFTRLFKQQTGMTPGEYLRQLRIEAARRRV
                     QELSGNQEAVAASVGFGSSRTLQRSLKPAVRAALRNN"
     gene            3438..>3677
                     /locus_tag="VGB93_05185"
     CDS             3438..>3677
                     /locus_tag="VGB93_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145538.1"
                     /translation="MTNHNEDARLDRRTFAKLSALGLSAAVVGVPGVTLAHTGIVSKP
                     EAFTALRDKPMNIGILIFPNMDQIDFTGPFEVLSRL"
BASE COUNT          773 a         1066 c         1060 g          778 t
ORIGIN      
        1 atctgccatt ccgatctgca ccaggcgcgc aaccagtgga acaacgcagt ctatcctatg
       61 gacccgggga acgagatcgt cgggcgcgtc gtcaaggtcg gggcgcacgt gaagaagctg
      121 caggtcggtg atttcgccgg ggtcgggtgc atggtcgatt cctgtcgcca atgctcggcc
      181 tgcaacgatg atctggaaaa ttattgcgag cataagccga cctatactta taatgaccgc
      241 gagcgcggcg gcgaccggct gaccttcggc ggctattccg aacagatcgt cgtcgaggag
      301 cgcttcgtcg tgaaggtgcc ggcaagcctc gatctcaagg cggtggcgcc gttgctctgt
      361 gccggcatca ccacctattc gccgctgcgg cattggaaag tcggcccagg cgacaaggtc
      421 ggcgtgatcg gcctcggcgg cctcggccat atgggcatca aattcgccaa ggcgctcggc
      481 gcgcatgtgg tgatgatcac cacgtcgccc ggcaaagcac aggatgcgaa aaagctcggc
      541 gccgacgagg tcttgatctc aaccgatgcg gcggcgatgc aggcgcacgc ggcgagtttc
      601 gacttcctcc ttaataccgt gccggtgccg cacgacctca atccctatat cgcgctgctg
      661 aagcgcgatc gcgcaatggt gctggtcggc gtcctgaccg agcttgatcc gccggtccaa
      721 ggcggcggcc tcatcatggg ccgcaagagc gtcgccggct ccgccatcgg cgggatgaag
      781 gagacgcagg agatgctcga cttctgcggc gagcataaca tcgtcgccga catcgagatg
      841 gtcgacatcc agacggtcaa cgaggcctac gagcggctca tcaagaacga cgtgaaatac
      901 cgcttcgtca tcgacatgga ttcgctgcgc aaagaagcgg cgtaaaggcc gaaacattcc
      961 ggcgccgcgc tccgaacgca cagacttcat ggcgaatatc aacatcgccg atctcgtccg
     1021 cgagatcggc gatggcacgt acctcacgcg cggaaaatga tctccgatcc ggtataacca
     1081 cggatcagtt ttgtcggcag ctccgctttc atgatgtcgt agaacaattc gccgctgcaa
     1141 tggagcggaa taacataatc aggattgact gccgccagct ctttgatcgt ctcgcgaata
     1201 taatcttcct tttggggtgc cagatgaaag cccccgataa tcgcgtgaat cttctcgacc
     1261 ccggaggcac tttgcgtctg cttgatcgtg ttgaccaaac cgcggtgact acaagaagtt
     1321 aatatgatca gcccccgccc ctttaaattg aaggcggtcg cgatttcatg gcgaaactgg
     1381 tccggaataa ttcccttatt tctctcgtcc tgtgtgaagt cctccggata gcaaccgaac
     1441 gaacctgtcc tgccgatctt cattcggctc ggtgaaagca ctttctcaaa agatgtcagt
     1501 tcgattcgtc ccgtcgtgaa cccatgatca gcaacgacgg cgggtccagg cgcaaacatg
     1561 atcgacagat tggctttctc aatcgcatct cgattcaacg cgccaaaatc gcctttcatg
     1621 ggtggagcta cccattggcg cgcacagaaa cattcctcac cgccgagaaa gattggtaac
     1681 ttcggcttga gctttccttt ggaatgatcg aggaagccga ccagtccgcc gaaatgatcg
     1741 tagtgcccat gcgataagac aagcgcgtca atcgaacccg gatctagacc gagaagatcg
     1801 aaattgttca gtagcgtctg cggcgtataa ccgaaatcga tcaacacccg gcgtgaatca
     1861 ccgccgatta gcgatgtcgc atggagcgac agcccgaact cgctcagaat tgctttttcc
     1921 ggcggatgat cgcctacagc aaatccaaag cgctttattt ccaggtttcc aactttgatg
     1981 ttgggcgcca cggccatttg gtagctgtcg gtaacgatac gaaccgacag ttgatcgatt
     2041 tccggcggct tgttttgaag tgccgcggct tggactgtct cgcttgttga gaatagcaac
     2101 gaaatgaaac tgctgaaggc gaatgccccg ccgctgcaaa taacgtcgcg gcgatctgct
     2161 gaaatggaat tgttaggcaa agtcattggt gcctccttcg tctcgcctgc gagaagccgt
     2221 tgagaggagc agcgaagttt gtaggacgtt atcctttttg tcgtcagcag gccgagtgaa
     2281 ccaaggttcc accccgaggc ctgaaaagca atacggactc gctgcgcaaa gaagtggctt
     2341 agccttagct cgggcagcaa agttggcgcg atctgtccgg tttttgtcct ggcccgcgag
     2401 atggcacggc cgtaacttca tggcgttagg acaaagccgg acggacgttt aagatggccc
     2461 ccaagacagt tctcatcatc gccccacccc gcgtcacgct tcaagacgtg accggtccgt
     2521 gggaagtctt ctgtcgtgca gaagggctga gacccggcac ctatgatgtc gcggtggttt
     2581 ccgcgggatt gcagaagaag gtcgagacga aattcggctt aggaatcgtc tgcgaacgat
     2641 ctgtccgcga tttcgccggg cccctcgata cagttctggt cgccggcagc gacgaaggtg
     2701 tatcgggcga ggctgaccct gctttcctcg attggctccg cgatgcggcg acacgcacgc
     2761 ggcgcatggg atcgatctgc acggggtcgt tctatctcgc acacgcaggt cttctcgcgg
     2821 gacgccacgc gacaagccat tggcgctatc tggataggct tgcggattca tttccagatg
     2881 tttcggtcga gcgcgatcct atttttgtcc gtgacggcga tatctacacg tccgccggca
     2941 tcaccgctgg gatcgatctc gcactcgctt tggtagaagc ggattgcggg catgatgtct
     3001 ctcaggccgt ggcacgcgat cttgtggtct ttttgcagcg gcaaggcgat cagccgcaac
     3061 tcagcacggc gctcgcgctg cgcatggccg atcgcgatcc gatccggtcc ttgcagcaat
     3121 ggatgccgga taatctccaa tccgttgtgc gggtcgagga catggcggct tacgtccaca
     3181 tgagcccgcg caatttcacg cgtctgttca agcagcagac cgggatgacg cccggcgagt
     3241 atctccgaca attacgcatc gaagccgcgc ggcgccgtgt tcaggaattg tcgggcaatc
     3301 aggaagccgt ggcagcttcc gttggattcg gcagcagccg aaccttgcag cgatcgctga
     3361 aacccgcagt ccgtgcggcg ctgcgcaaca actgacagca aaccagcttt cccttccccc
     3421 taatcaatgg gtgtcctatg acaaaccata atgaagatgc acgcctcgat cgtcgcactt
     3481 tcgcgaagct ttcggccctc ggcctgtcgg cggctgtggt cggggtgccg ggcgtgacgc
     3541 tcgcgcatac cgggatcgtc tccaagcccg aagcctttac ggcgcttcgc gataagccta
     3601 tgaatatcgg catcctgatc ttcccgaaca tggatcagat cgacttcacg gggccgttcg
     3661 aagtcctgtc gcgcctg
//
LOCUS       DASWOG010000255         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_134452,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000255 DASWOG010000000
VERSION     DASWOG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134452"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VGB93_05190"
     CDS             <1..108
                     /locus_tag="VGB93_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEY0145539.1"
                     /translation="KGDEEKIRQGGCEAYLSKPISVVKFLETVRSYIAE"
     gene            130..1500
                     /locus_tag="VGB93_05195"
     CDS             130..1500
                     /locus_tag="VGB93_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEY0145540.1"
                     /translation="MTAKILVVDDILPNVRLLEARLTAEYFDVTTAMNGAEALAVCAQ
                     DDCDLILLDVMMPGMDGFEVCRRLKKNPATTHLPIVMVTALDQPSDRVKGLEAGADDF
                     LTKPIDEIALIARVRSLTRLKVVLDDLRNRASTAVALGMPDPFTAALAEDGLGGRILL
                     VDDRSASAAQLAFMLRDKYIVEIEPNPNDVAAKVASGAYDMLIVSLGLHGFDALRLCG
                     QLRSQERTRQLPILCLAEMEDRTRVLRALDLGVNDFLMRPVDGNELIARVRSNLRRKR
                     YADKLRDNVQASLELAVVDPLTGLNNRRYFEMHLSILVKQAVDCDRPLSMMILDIDHF
                     KGINDTYGHATGDQILKGFAKRVRKVIRIPDVICRIGGEEFVVVMPDTNMEVAARIAE
                     RVRAEIEQERFVVPHSGAAVGITVSIGLAERGDDDLPDGLFSRADRALYRSKSNGRNR
                     VSADAA"
     gene            complement(1905..2072)
                     /gene="rpmG"
                     /locus_tag="VGB93_05200"
     CDS             complement(1905..2072)
                     /gene="rpmG"
                     /locus_tag="VGB93_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY0145541.1"
                     /translation="MAKAAMIKIKLQSSADTGYFYVTKKNARTKTEKLSFKKYDPVAR
                     KHVEFKETKIK"
     gene            complement(2195..>3341)
                     /locus_tag="VGB93_05205"
     CDS             complement(2195..>3341)
                     /locus_tag="VGB93_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145542.1"
                     /translation="KTHSETHAFVVAAAITTVMIAAIGLSLSATLIAVRLAQAGYSAR
                     AIGLHPAASSLVGLVTAVYVPWLARIIGVRRLLIYALLLGAVCLAGLSLTHGYWPWLA
                     VRCVYGIALTVLFILAEFWISAIVWPRRRGLVMGIYATAVAAGFAAGPFVLAATGPSG
                     ATPFMIAALLSLAAIMPIGLAGAEAPALEEAQPLPFKAILRGAPAATAAAFIFGAIEA
                     AAFGLFPVFALREGWGAGAAAVATAIFALGNALFQIPTGFLADRFGQRRLLILMGALG
                     TIGALILPLAARSSYASYVMLLFAWSGIVGGLYAVGLTSLASRYPEAGALARANAAYI
                     LLYTAGSLVGPPIFGFSLDLAPSGLFFSLAVVLALFFALAMKRDANP"
BASE COUNT          703 a          945 c         1042 g          651 t
ORIGIN      
        1 aagggggatg aagaaaagat tcgtcagggc ggctgcgagg cttacctgtc gaagccgatt
       61 tcggtggtga aattcctcga aacggtgcgc agttacatcg ccgaataatc cggcggggga
      121 acgtttctga tgacggctaa gattctcgtt gtcgatgata ttttgccgaa cgtgaggctt
      181 ctcgaagcga ggcttacggc ggaatatttc gatgtcacga cggcgatgaa tggcgccgaa
      241 gcgctcgcgg tttgcgcgca ggatgattgc gacctcattc ttctcgatgt gatgatgcct
      301 ggcatggacg gatttgaggt ctgccgacgg ctcaaaaaaa atccggcgac gacgcatctg
      361 ccgatcgtga tggtgacggc gctcgatcag ccaagcgacc gggttaaggg cctcgaggcc
      421 ggtgcggacg attttctgac aaaacccatc gatgaaatcg cgctcattgc gcgcgtacgt
      481 tcgctcaccc ggttaaaggt ggtgctcgac gatctacgta atcgcgcttc gactgccgtc
      541 gctctgggta tgcctgatcc attcactgca gcgctggcgg aagacggcct cggtggccgt
      601 attctgcttg ttgacgatcg ttccgcttcg gcagcgcaac tcgccttcat gttgcgcgac
      661 aaatatatcg tcgaaatcga gcccaatccg aatgacgtcg cggcgaaagt tgccagcggc
      721 gcgtatgata tgttgatcgt gagtctcggg cttcacggct tcgatgctct gcgcctgtgc
      781 ggccaactcc gttcgcagga gcgcacacgc caattgccga tcctttgcct ggccgagatg
      841 gaagaccgca cgcgcgtgct tcgcgcgctc gatcttggtg tcaacgattt cctcatgcgg
      901 cccgttgacg gcaacgagtt gatcgcgcgc gtgcgcagca atctgcggcg caagcgctat
      961 gccgacaagc tgcgcgacaa cgtgcaagct tcgctcgaac tcgcggtggt cgatccgctg
     1021 accgggctca acaaccgccg gtatttcgag atgcatcttt cgattctcgt aaagcaggcg
     1081 gttgattgcg accggcctct ctccatgatg atccttgata tcgatcattt caaaggcatc
     1141 aacgacacct acggacacgc gacgggcgat cagatattga agggctttgc gaagcgcgtc
     1201 cgcaaggtga tccgcatccc tgacgtcatc tgccgtattg gcggcgagga attcgtcgtc
     1261 gttatgccgg atacgaatat ggaggttgcc gcgcgcatcg ccgagcgcgt ccgcgccgaa
     1321 atcgagcagg agcgtttcgt cgtgccccac agcggcgcgg cggttggcat tacggtttcg
     1381 atcggcttgg ctgagcgggg tgatgatgat cttccggatg gccttttcag ccgcgccgac
     1441 cgggcgctct atcgctccaa atcgaatggc cgaaaccgcg tttcggcgga cgccgcctga
     1501 atcgttttgg caagcccacg gccaaaaaga ttagatattt aggtaaataa acggaggcgg
     1561 gacgtctcta caattaagca gttttctttt cgtgagccgc acgctaactt gaacatctcg
     1621 gtgacggcgc cgctgcgtta cgcgaatgac gcctcggtcg cgaatactaa atatttcaac
     1681 agaataccct acggagtgct caggtccgcc gcatctcctg ggtccgtggg gtcggccggc
     1741 gcgcaaatgg ttaggagtgg cctcctcgag ataccatttg ccgccggcca ccattcttcc
     1801 tgtcgaagga caagggtggc ggcggtgagt cgcgcgcctc ataaattaaa ttaagaaaat
     1861 agattatctt attgggcgga ctgctaaatt gcgtccgctc cgaatcactt gatcttggtt
     1921 tctttgaact cgacgtgctt gcgcgcgacc gggtcgtact tcttgaacga cagcttctcg
     1981 gtcttcgtgc gggcgttctt cttggtcacg taaaaatagc ccgtgtccgc cgaggactgg
     2041 agcttgatct tgatcattgc ggccttggcc atctgcggct tccttcaaac ctgagcgcgt
     2101 cgcgtggcga aaggcgccga cgcgttaaat gctgacgatg cgcgaaagtg cgcgcacctt
     2161 aaggatcggc cttcgcttgt caaggattgg cttgtcaagg attggcgtct cgcttcattg
     2221 ccaatgcgaa gaaaagcgcc agaacgacag ccaaactgaa gaagagaccg gagggggcaa
     2281 ggtcgagact gaagccgaaa atcggcggcc cgacgaggct ccccgcagtg tagagcagga
     2341 tataggcggc gttggcgcgt gccagcgcgc cggcctctgg atagcggctg gcgaggcttg
     2401 taaggccaac tgcgtaaagg cccccgacaa ttccgctcca ggcgaacagc agcatgacat
     2461 agctggcgta ggacgagcgc gcggcgagtg gcaaaatcag cgcgccgatg gtgccaaggg
     2521 cgcccatcag gatcagcaat cggcgttggc cgaaacggtc tgccagaaag ccggttggga
     2581 tctggaacaa tgcgttgccc agggcgaaga tcgcggtggc gactgcggcc gcgccagcgc
     2641 cccagccttc gcgtaaggca aagacgggaa aaagaccaaa ggcggcggct tcaatcgcgc
     2701 caaaaatgaa ggccgccgcg gtcgctgcgg gcgcgccgcg caagatcgcc ttgaagggca
     2761 gcggttgcgc ctcttccagt gccggcgcct ccgctccagc aagcccgatt ggcatgatgg
     2821 ccgccagtga gagcagggcg gcgatcataa aaggcgtcgc accagaaggg cctgttgcag
     2881 cgagaacgaa aggtcccgct gcgaagcccg ccgcaacggc tgttgcgtaa atccccatca
     2941 caagaccgcg ccggcgtggc cagacgatcg cgctgatcca gaattcggca agaatgaaga
     3001 ggacggtcag cgcgatccca tagacgcacc gcactgcaag ccaaggccaa tagccgtgcg
     3061 tgagcgaaag gccggcaagg cagacagcgc ccagcaatag tgcgtagatc aggagccgcc
     3121 ggacgccgat gatgcgcgcg agccagggca cataaaccgc tgtgacgagg ccaacgaggc
     3181 tgctcgccgc gggatggagg ccgatggcgc gtgccgaata gccggcctgc gccagccgca
     3241 cggcgatcaa agtcgccgac agcgacagac ctatagcggc gatcatcacc gttgtgatgg
     3301 ccgcagcgac aacgaacgcg tgcgtttcgg aatgtgtttt c
//
LOCUS       DASWOG010000256         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_226074,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000256 DASWOG010000000
VERSION     DASWOG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226074"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGB93_05210"
     CDS             <1..813
                     /locus_tag="VGB93_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0145543.1"
                     /translation="VATIESLKGPASLSRFDGARSMNVEADLTDGAALGDVEKALDAL
                     PIMKHMPPGVRAHKSITAEAMQDLSSGFAEAMRHGLIMVYIVLMLLFARVLQPITILF
                     SLPLSIGGAIVALLITGRPLSLPVIIGILMLMGIVTKNAIMLVDFATRAMARGMPRTA
                     AIAEAGTKRARPIVMTTIAMVAGMVPSALGLGAGGEFRSPMAIAVIGGLIASTFLSLL
                     FVPAVFAVMDDVRNAGFAGLHALIAVFRGLPKLHDLRFAFTRRPRPVSGPAE"
     gene            840..1811
                     /locus_tag="VGB93_05215"
     CDS             840..1811
                     /locus_tag="VGB93_05215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0145544.1"
                     /translation="MKENWPRQAPVVALDAAAVERLVLPLFPGDSVRSFAPVGGGLTN
                     TNYKVTLARRAAPLLLRLYQRGVAPAQKEMAIDTLIAPRVPVLHFFHLGEDNPVTGHP
                     YAVLDWIDGPDLQHILPGLSQDRLLAFAGRVGKVLAAIHSFTFEIFGFFDAKLSVQGP
                     IDFDRAGMLAYLDQTLVRGSGGERLGRPLVDALIAYAEKNGHILKDWLKQPCLVHGDF
                     NAPNILVLPERDDAIAAVIDWEYALSASAAMDFGNLLRPPFDGNAAFAEAVARAYVEA
                     GGFLPKNWQRIARLADVYSFADFLSRPYVADVVIKDARQVIAQLVAD"
     gene            complement(1817..2959)
                     /locus_tag="VGB93_05220"
     CDS             complement(1817..2959)
                     /locus_tag="VGB93_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145545.1"
                     /translation="MRSRPCILAVDDVFENLEIVTLRLEANGYEVITAMDGIEALEQT
                     RRHLPDMILLDIMMPKLDGISVLKELKADPTLGFIPVLLLTAKADRSDVVAGLEAGAD
                     DYLTKPLDQAALIARVRSMLRIKALHDKVQDQARQLAIQAEELAEWNRGLEARVAEQV
                     SEIESISRLKRFLAPQVVDVIALSGDPDALLASHSAEITVLFCDLRGFTAFTEAAEPE
                     QVMKVLSEFHATLGNLIHHYEGTLERFAGDGLLTLFNDPLPCPDHSERAVRLAIDMRA
                     GLQGLSQGWRQRGHELGFGIGIARGIATLGKIGFDRRFDYAAIGSVTNLASRLCDEAK
                     SGQILIDRNAFAATENLFEAERLPPLSLKGFSRQIEAFAILGARSG"
     gene            complement(2996..3361)
                     /locus_tag="VGB93_05225"
     CDS             complement(2996..3361)
                     /locus_tag="VGB93_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145546.1"
                     /translation="MTKRILVVEDTEDNRQILRDVLSDAGYDLIEAGDGEEGVRMAAE
                     FQPDLVLMDIQLPLVDGYEATRRIKADPVSRHIPVIAVTSYALAQDEKRALAAGCSGY
                     VAKPFSPRALLAKIREFLP"
     gene            complement(3358..>5257)
                     /locus_tag="VGB93_05230"
     CDS             complement(3358..>5257)
                     /locus_tag="VGB93_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145547.1"
                     /translation="THHCPTIQISVALSGKQPGVSVATVELKFLSDILNGIQAAAGMY
                     AYIANTKGDLIAHTDSSMLLHHPDLLRLPQVAALARDPEGTVSVGRSLDGQSVLAASS
                     PVSSMQWWLFVEQPLAQAYAPVTDLLWRLFIILVLALVLCIGAGLILARWMTVPIQAV
                     RDGAAHLAEGDFTHKIEVHTGDEIEDLADDFNLMASQLQDSYAKLEHKVEERTRDLER
                     SVSELRALEEVGLALAASLNLNEVLATIVVRSVEVTGAHGGAIYSFDPARKAFNLAEA
                     YGLDPDFVAAIREVKIRRPHGLLSEVAERKVPIQIPHIAAARDFPLRGATLAAGLQSA
                     LIAPLIGSAELLGVLVIERRESGYFPDNTVALIKTLANQSVLAMSNAKLFEQVQEKNK
                     QLAIANEHKSLFFANMSHELRTPLNAVLGYTELMQDGIYGEVPDRAKQVLLRIQTNGA
                     HLLSLINDVLDISKIEAGELSLSLSDYSLRAVIETVVNATGSLAQAKGLRISTDIAPD
                     LPIGRGDERRLTQVLLNIVSNAIKFTDKGGVMIRAKASGNDFKLAVKDTGPGIAPEDQ
                     ARIFEAFHQVDSTITKQKGGTGLGLSISKRFIEMHGGVIGLTSTLGEGSTFKVQIPVR
                     VDRQMEAA"
BASE COUNT          971 a         1561 c         1640 g         1085 t
ORIGIN      
        1 gttgcgacga tcgagtcgtt gaagggcccc gcgagcctca gccggttcga cggcgcacgc
       61 tcgatgaatg tcgaggcaga tctgacggat ggcgctgcgc tgggcgatgt cgaaaaggcg
      121 ctcgatgcgc tgccaatcat gaagcacatg ccgccaggcg tgcgggcgca caaatccatc
      181 acggccgaag cgatgcagga tctgagttcc ggcttcgccg aggcgatgcg ccacggcctc
      241 atcatggtct atattgtgct gatgctgctg ttcgcccgtg ttctgcagcc gatcacgatc
      301 ctcttttctc tgccgctctc gatagggggc gcgatcgtcg ccctgctcat cacgggccgt
      361 ccgctgtcgc tgccggtcat cattggcatt ctgatgctga tgggcattgt gacgaaaaat
      421 gccatcatgc tggtcgattt cgccacccgc gcgatggcgc gtggcatgcc acgcacggcg
      481 gccattgcgg aggcggggac gaagcgggca cggccgatcg tgatgacaac gatcgcgatg
      541 gtcgcgggca tggtgccgag tgccctcggc cttggcgccg gcggcgaatt ccgctcaccc
      601 atggcgattg cggtcatcgg cggcctgatc gcttcgacgt tcctgtcgct gctcttcgtc
      661 ccggcggtct tcgcggtgat ggatgatgtc cgtaacgccg ggtttgcagg tctgcatgcg
      721 cttatcgcgg tatttcgcgg tttgccgaaa ttgcatgatc tgagatttgc ctttacacgc
      781 agaccccgcc cggtcagcgg accggccgag tagccagaaa tccagcaggc gtctgcggaa
      841 tgaaagaaaa ttggccccgg caggcgccgg tcgttgcgct cgacgcggcc gctgtggagc
      901 ggctcgtact gccactcttt cccggcgatt ctgtccgcag cttcgcgccg gttggcggcg
      961 gtctgaccaa cacgaactac aaagtgacgc tcgcgaggcg ggcggcgccg ctgctcttgc
     1021 ggctttatca gcgtggcgtc gcgccggccc agaaggaaat ggcgatcgat actttgatcg
     1081 cgccccgtgt gccggtcctg catttttttc atctgggcga ggataatccc gttaccgggc
     1141 atccctatgc tgtgctcgat tggatcgacg gccccgatct tcagcatatt ctgcccggct
     1201 tgtcgcaaga ccggctgctc gcgttcgcgg gcagggtcgg caaagtgctc gccgcgattc
     1261 atagtttcac gttcgagatt tttggctttt ttgacgccaa gctcagtgtg caaggaccga
     1321 tcgatttcga tcgcgctggg atgcttgcgt atctcgatca gacgctcgtc cgtggatctg
     1381 gtggcgagag gctggggcgc ccgcttgttg atgcactcat cgcctatgcc gaaaagaacg
     1441 gccatattct aaaggattgg ctcaagcagc cctgcctcgt gcatggcgat ttcaacgccc
     1501 cgaatattct cgtgctcccg gagcgggatg acgcgatcgc ggcggtgatc gattgggagt
     1561 atgcgctgag cgcgtcggcg gcgatggatt tcggcaatct gttgcggccg ccgttcgacg
     1621 gcaacgcggc gttcgcggag gcggtggcgc gtgcctatgt cgaagctggc ggtttcctgc
     1681 cgaagaattg gcagcggatc gcccggcttg cggacgttta ttcctttgcc gatttcctga
     1741 gccggccgta tgtcgccgat gtcgtcatca aggatgcgcg acaggtgatc gcgcaacttg
     1801 tcgccgactg acggcgctac cccgagcggg cgccgagaat cgcaaaagct tcgatctgac
     1861 ggctgaaacc tttcaaggac aatggcggca gacgttcggc ctcgaacaga ttttccgtgg
     1921 cggcaaaggc attgcggtcg atgagaatct ggccggactt tgcttcgtcg cagaggcgcg
     1981 aggcgagatt ggtcacgctg ccgatcgccg cataatcgaa acgccgatcg aagccgatct
     2041 tgcccaacgt cgcgatgccg cgcgcgatac cgatgccgaa gccgagttcg tgtccgcgtt
     2101 gtcgccagcc ctgcgacagc ccttgaagtc ccgcccgcat atcgatcgcg agccgcaccg
     2161 cgcgctcgct atgatcgggg caggggagag gatcgttgaa gagcgtcaaa agcccatcac
     2221 cggcgaaacg ctccagcgtg ccttcgtaat gatgaatgag attgcccagt gtggcgtgga
     2281 attcgctcaa gactttcatc acctgctcgg gttcggccgc ctcggtaaaa gcggtgaatc
     2341 cgcgcaaatc gcagaagaga acagtgattt cggcgctatg gcttgccagc aacgcatccg
     2401 gatcgccgga aagcgcgata acgtcgacga cctgcggcgc cagaaagcgt ttgagccggc
     2461 tgatactttc tatctcggag acttgttcgg cgacgcgcgc ttcgagcccg cggttccatt
     2521 cggccagttc ttcagcctga atcgccagtt ggcgcgcctg atcctgtact ttgtcgtgga
     2581 gggccttgat ccgcaacatc gagcgcacgc gcgcgattag cgccgcctga tcgagcggct
     2641 tcgtcagata atcgtccgcg ccggcttcta ggcccgcgac gacatcgctg cggtcagcct
     2701 tcgcggtcaa aagcagaacg ggaatgaagc ccagtgtcgg gtcggcctta agctccttca
     2761 agaccgaaat gccgtcgagt ttcggcatca tgatgtcgag caggatcata tcgggcagat
     2821 gccggcgcgt ttgttcgagc gcctctatgc catccatggc cgtgatcact tcatagccgt
     2881 tggcttcaag ccgcagcgtg acgatttcga gattttcgaa aacgtcgtca acggcaagga
     2941 tgcaagggcg actacgcaat ctatggcctc tcagcttcct gcatttgcgc cccggtcatg
     3001 gcagaaattc gcggattttg gcgagcaatg cacgcgggct aaacggcttc gcgacatagc
     3061 cgctacagcc cgcagccaag gcccttttct catcttgcgc gagcgcatat gacgttaccg
     3121 cgatgacggg gatgtggcgc gagacagggt ccgctttgat gcgccgcgtc gcctcatagc
     3181 catcgacgag cggaagctgg atatccatca aaacgagatc gggctggaat tcggcggcca
     3241 tccgcacgcc ttcttcaccg tcgcccgcct cgatgagatc gtagccggcg tcgctcagca
     3301 cgtcgcgcaa aatctggcgg ttgtcctcgg tgtcctcgac gacaagaatg cgtttggtca
     3361 tgccgcctcc atctgccgat cgacgcgcac cggaatttgt actttgaatg tagagccttc
     3421 gccgagcgtg gatgtgagac cgatcacacc tccgtgcatt tcgataaacc gcttgctgat
     3481 ggaaagcccc agaccggtgc cacccttttg cttggtgatc gtgctgtcga cctgatggaa
     3541 ggcttcaaag atccgcgcct gatcctcggg tgcgattcca gggcctgtat ccttgacggc
     3601 gagcttgaaa tcgttgccgg aggccttcgc acgaatcatc acaccgccct tgtctgtgaa
     3661 tttgatggcg ttgctgacga tgttcaagag cacctgcgtc agccggcgct cgtcgccgcg
     3721 tccgatcggc aggtccggcg cgatatcggt ggaaatccgc agccccttgg cctgtgccaa
     3781 cgagcctgtg gcattgacga cggtctcgat cacggcgcgc agcgaataat cgctgaggga
     3841 cagggagagt tcacccgcct cgattttgga aatatcgagc acatcgttga tgaggctgag
     3901 aaggtgcgcg ccgttggtct gaatacgcag gagcacctgc ttggcgcggt ccggcacctc
     3961 gccatagatg ccgtcctgca taagttcggt gtatccaagg acagcattga gcggcgtccg
     4021 caattcgtgg ctcatattgg caaagaacag cgacttgtgc tcgttagcga tggcgagctg
     4081 cttattcttt tcctgcacct gctcgaacaa tttggcgttg ctcatcgcca gcacggattg
     4141 gtttgcgagc gtcttgatca gcgcgacagt attgtcaggg aaatagccgg attcccggcg
     4201 ctcgatgacc agcacgccga gcagttccgc cgaaccgata agcggcgcaa tcagcgccga
     4261 ttgcagaccg gccgccagcg tggcgccgcg cagcgggaaa tcgcgggccg ccgcaatatg
     4321 cgggatttga atcggcacct tgcgttccgc aacttcgctt aaaagtccgt gcgggcgccg
     4381 gatcttcacc tcgcggattg cggcgacgaa atccggatcg agcccgtagg cctcggcgag
     4441 attgaacgcc ttgcgcgccg gatcgaaact gtagatggcg ccgccatgtg cgccggtcac
     4501 ctcgacggag cgcacaacga tcgttgccag aacctcattg agattaagcg aggcggcgag
     4561 ggcgagaccg acttcctcga gcgcccgcag ctcgctgacc gagcgttcga gatcgcgggt
     4621 gcgctcttcg accttgtgct cgagtttggc gtaggaatcc tgaagctggg acgccatgag
     4681 attgaaatcg tcggcgaggt cttcaatctc gtcgcccgta tgcacctcga ttttgtgcgt
     4741 gaagtcgcct tcggcgagat gcgccgcgcc gtctcgaacg gcctgtatag gcaccgtcat
     4801 ccagcgcgcc aggatcagcc cggccccgat gcagagcacg agcgccagca ccaggatgat
     4861 gaacagccgc cacagcaagt ccgtgaccgg cgcataggct tgggccagtg gctgttccac
     4921 gaacagccac cattgcatcg aggagacggg ggaggaggcg gcgagaacgg attgtccatc
     4981 aaggctgcgg ccgaccgaca ccgtaccctc cggatccctc gccagtgctg cgacttgcgg
     5041 caatctcagc aggtccgggt gatgcagcag catcgaggaa tcggtatggg cgatcagatc
     5101 gcctttcgtg ttggcgatgt aagcgtacat gccagcggcg gcctggatgc cgttcaggat
     5161 atcggagagg aatttcaact cgaccgtcgc gacggagacg cccggctgct tccccgacaa
     5221 cgcgacggaa atctgaatcg ttgggcaatg gtgggta
//
LOCUS       DASWOG010000257         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_299602,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000257 DASWOG010000000
VERSION     DASWOG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299602"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGB93_05235"
     CDS             <1..193
                     /locus_tag="VGB93_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEY0145548.1"
                     /translation="VTSIVLQLDEKNPQVAARLLGAFKSWRALETHRRLLAENALRRV
                     ASHGRLSADVKDIVERSLA"
     gene            317..2713
                     /locus_tag="VGB93_05240"
     CDS             317..2713
                     /locus_tag="VGB93_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145549.1"
                     /translation="MIRADAAGLLARADLFYARRPKASRLKASDQTYIKFEPWLRLAV
                     PALIGLFVVALSATLIIILLDAHDRAIAAAVDDLELVAAATASDLAGLAKASDRDPSV
                     ALLHALPARALTRGRRIFISNKKGNIVAALPNALHHGGALADCLGSGRLLTSLAERGG
                     VLRVHLDDGTDALAIVRTLRAPYGQIAFVHPMSAVLADWRDGVLRMAAALVATIVVLL
                     SVAAAYFWQAARARKADIFCNRIRDRIDTALNRGRCGLWDWDLARGHVYWSDSMYDML
                     GMKPERAFLSFGEINALVHPHDGDLAHVAEMLAASQAKAVDHSFRMRHADGHWIWLRA
                     RAEIVREGPGEAAHLVGIAVDTSEQKILAERTATANMRLRDALETVSEAFVLWDKENR
                     LVMCNSKFQRFHNLPDTAIISGQPYQQVMAQGTPPQVQSQFALGERPAIGARTYEAQL
                     GDGRWLQIDERRTKDGGYVAIGTDITALKQHESQLLDSERRLTATVADLHKSRQTLEH
                     QAQQLAELAEKYLEQKAEAESASRAKSEFLANMSHEFRTPLNAIIGFSDMMIGEVFGA
                     LPARYLDYSKDIRSSGQQLLALISDVLEMSRLDAGRVQLQKAEVTIGALVSAALKSIE
                     AAAREKSIALTSQIAASVRLHADPAALERVIAALLHNAVKFTPDHGSVNIRARHVNGA
                     LNIYVEDNGVGIAPEALERLGRPFEQWNAPLANGMKGSGLGLAIARSLVALHDGTIRI
                     RSTPGNGTIVQIHLPVVKPVCAVASKPAAARRSLTSVQPHLAGRTISRPEVQVSRRPH
                     "
     gene            complement(2694..>3031)
                     /locus_tag="VGB93_05245"
     CDS             complement(2694..>3031)
                     /locus_tag="VGB93_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0145550.1"
                     /translation="DFLAQYLCLKHAHAAPMLLAGAPADIFMRAQSLGFLAVADAASL
                     LAADQLYAHVIQVLRAILEGNVRLVQAGDAVKRRLATALNLPSFGQLEAELLETEARV
                     RAIFNAAVG"
BASE COUNT          592 a          925 c          913 g          601 t
ORIGIN      
        1 cgtgacgtcg attgtcttgc agctcgatga gaaaaatcca caggtcgcgg cgcggcttct
       61 gggcgctttc aaaagctggc gggcgcttga gacgcatcgg cgtttgctgg ccgaaaatgc
      121 gctgcgtcgc gttgcatcac acgggcgtct gtcggcagac gtcaaggata tcgtcgagcg
      181 ctcgctcgcc tgacctaatc aattttcttt tcaggattca ttaatctctg gacaaatcac
      241 cgctctcgga ttcagataca cgggattcga agagatgcgg cacatcgact tcggatcgta
      301 tcgagggggc tccacgatga tccgtgcgga tgcagcgggc ctgttggcac gtgctgatct
      361 gttctacgcc aggcggccaa aggcgtctcg cctgaaggct tccgatcaaa cctatattaa
      421 gtttgagcct tggctgagat tggccgttcc ggcgctcatc ggtctcttcg tggtcgcgtt
      481 aagcgctact ctcattatca tcttgctcga cgcgcacgat cgcgcaatcg ccgcggcggt
      541 tgacgatctg gagcttgtcg ccgccgcgac ggcgagcgac ctcgccggcc tcgcgaaagc
      601 gtcggatcgc gatcccagtg tggcattgct acacgccctc cctgcccgcg ccctgacgcg
      661 cggtcgccgc atcttcatca gcaataagaa aggcaacatt gtcgcggcgc tgccgaacgc
      721 gcttcaccac ggcggcgcgc tagccgattg tctcggcagc gggcggttgt tgacgagtct
      781 ggcggaacgc ggtggtgttc tgcgtgtcca tctcgacgac ggcacggatg cactggcgat
      841 tgtgcgtacg ttgcgagcgc cgtacggcca gatcgctttt gtgcatccga tgagcgccgt
      901 gctggctgat tggcgcgatg gtgttctccg aatggccgcg gcactcgtag ccacgatcgt
      961 cgtgcttctg tccgtcgctg cggcctactt ctggcaagcg gctcgggcac gtaaggccga
     1021 tattttctgc aatcgcattc gcgacaggat cgatacggcc ctcaatcggg gccgctgcgg
     1081 actttgggat tgggatctcg cccgcggcca cgtctattgg tcggattcga tgtacgacat
     1141 gctcggcatg aagccggagc gagcctttct ctccttcggc gagatcaacg cgctcgttca
     1201 cccgcacgac ggagatctcg ctcacgtcgc tgaaatgctc gccgcctcac aggccaaagc
     1261 tgtcgaccat tcgttccgga tgcgccacgc cgatggccat tggatctggc tgcgggcgcg
     1321 cgccgagatc gtgcgcgagg gaccgggcga agcggcgcat cttgtcggca tcgccgtcga
     1381 tacatcggag caaaagatcc tcgccgaacg gacagctacg gcaaacatgc ggctgcggga
     1441 tgcgctggag accgtctccg aagcctttgt tctttgggac aaagaaaatc ggctggtcat
     1501 gtgcaattca aagttccagc gatttcacaa tcttcctgac accgctatca tcagcgggca
     1561 accttatcag caggtgatgg cgcaaggaac gccgccgcag gttcaatcgc aattcgccct
     1621 cggcgaacgg cccgccattg gcgcaaggac ttacgaagcg caactcggcg acggccgatg
     1681 gctccagatc gacgagcgtc gcaccaagga tggcggctat gttgcgatcg gcaccgacat
     1741 cacagccctt aagcagcacg aaagccaatt gctggactcc gaacggcggc tgacggcgac
     1801 ggtcgcggat ttgcacaagt cgcgtcagac cctcgaacat caggcgcagc agctcgccga
     1861 actcgccgag aaatatctcg aacaaaaagc cgaggccgaa tccgcaagcc gggcgaaatc
     1921 cgaatttctc gccaacatga gccacgaatt tcgcacgcca ctcaatgcga tcatcgggtt
     1981 ctcggacatg atgatcggcg aagttttcgg cgcactcccc gcgcgctatc tcgattattc
     2041 caaggatatc cgaagcagcg gtcagcaatt gctggcgctg atttcggatg ttctcgaaat
     2101 gtcgcggctc gacgcgggac gcgtgcaatt gcagaaagcc gaggttacga tcggtgcgct
     2161 tgtttccgcc gcgctgaaga gcatcgaggc ggcggcgcgc gagaagtcga ttgcgcttac
     2221 aagtcagatc gcggccagcg tcaggcttca tgccgatcct gcggcactcg aacgggtcat
     2281 cgcggcgcta ttgcacaatg cggtgaaatt cacccccgac catggcagcg tgaatatccg
     2341 tgcgcgtcac gtcaatggcg cgttgaatat ctatgttgag gacaatggcg ttggtatcgc
     2401 accggaggct ttggaacggc ttggccggcc gttcgagcaa tggaatgcgc cgctcgccaa
     2461 cggcatgaag ggctcgggcc tgggcctcgc catcgcccgc tcccttgtcg cattgcatga
     2521 cgggacgatc cgcatccgct cgacaccagg caacggcacg atcgtgcaga tccatcttcc
     2581 cgtcgttaag cccgtctgcg ctgtcgcgtc caagccggcg gcggcacggc gctcgctgac
     2641 cagcgttcag ccgcatctgg ccgggcgtac gatatcgcgg cctgaagtgc aggttagccg
     2701 acggccgcat tgaagattgc gcgtacccgc gcttcggttt ccaaaagctc cgcctcaagc
     2761 tggccgaagc tcggcaggtt cagcgccgtc gcgaggcggc gcttcacggc atcgccagcc
     2821 tgcaccaatc gaacattacc ttcgaggatc gcgcgcagca cttgtatgac gtgtgcgtaa
     2881 agctgatcgg ccgccaaaag actcgccgca tcggcgaccg ccaaaaaacc cagcgattgc
     2941 gcgcgcatga aaatatccgc cggcgctcct gcaaggagca ttggagccgc atgtgcatgt
     3001 ttcagacaaa gatattgcgc gagaaaatcg a
//
LOCUS       DASWOG010000258         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_299647,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000258 DASWOG010000000
VERSION     DASWOG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299647"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGB93_05250"
     CDS             complement(<1..511)
                     /locus_tag="VGB93_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0145551.1"
                     /translation="MFIRWAGAFSGAALWAIGATAATANPAPAQTVTIYAAGSLKAFV
                     SLLAKANDPSLAGLDFKPTFKSAGLLRAQIEAGEEPDLFLSADMNAPRRLAAEGRALL
                     PPVAFARNRMCFYVPRRLGVTAGNLVARLLTASLRIKTSAPVADPGGDYAVAIFDRLD
                     HLHPDAGNIL"
     gene            complement(512..2515)
                     /locus_tag="VGB93_05255"
     CDS             complement(512..2515)
                     /locus_tag="VGB93_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0145552.1"
                     /translation="MADTPQSVTIVPESLIENAEAQTVNDCLRYLPSVEVRDQQGSEV
                     SRPQSRGFEGSIVQNVRLDGLNYIGTSAIDCENLRGVEVLNGLAGALYGPESPSGVFN
                     YLLKHPTDQSFFTYREDYDSQSIFTEQIDAGGRVGQDGRLGYRVNIVHGAGEGYVDDS
                     KTDRTLGSIDLDYRFDNNTDVEGYYGHYATDITGMPGEIVFGSSQTSKTNHSTFLPQA
                     VNPTQPGLGQPGAGDNLVSDTGMLKIRHEINQNWNFEAGGLYENANRGLFGITDILQD
                     NSGNYNVVKNFTAVPRFTVGSNSAYLNGRVWLYGMENDVALGTNGFINGQYNYNPAVA
                     TPTIGTGNLFAPTVLTSIPSFPAHGPEYEAGKIFSQSIITGDTLHFNRQWALQAVLST
                     AWIHSSSFNPTGATTSSTGANATLSPTISLIYNPISPLTTYFTWANSTELGDQAPTSG
                     VTNPSVFLSPYHDTMYELGAKYAFNPNFLVTVDGFRMTRPLAESVVTGPTTSIFEVTG
                     TQTNWGGEVFAQGDITPDISAFGGVTYIDARLVNTGNVATNDMLVIGVPHWKSDIAID
                     LHPDYWRGFAVTGTIHYESSRAANNTNTTFADPYATLDLGARYSTVIYGHRTTARFQV
                     VNVTDKFYYASIADGASIVGANGGNTAWLGTPRTFEASVEVNF"
     gene            2912..3727
                     /locus_tag="VGB93_05260"
     CDS             2912..3727
                     /locus_tag="VGB93_05260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0145553.1"
                     /translation="MLTRALLISGMASGSNRAILEGSNWEDHVPQLAEGLRIYAIGDV
                     HGRADLLERLFALIDADIEQRPVERAIQVMLGDYVDRGPASREVIDLILARAERHELV
                     ALKGNHDALLLQAIDDPAAMGDWLMMYGIETLASYGLTSATVAGSRLSDLGRAFAAAL
                     PEAHRKFFRELKLSYSHGDFFFVHAGVRPGIDLSLQAEEDLIWIRQEFLRYGGDFGKI
                     VVHGHSPVRDVERHANRIAIDTAAYASGKLTAIVIEGSDLRIIDTAEADAHAA"
     gene            3830..4153
                     /locus_tag="VGB93_05265"
     CDS             3830..4153
                     /locus_tag="VGB93_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145554.1"
                     /translation="MQFKGEYKDVLATRETMAREKMRQAVLCAFCHVVQTTGLKPMAV
                     LGLAASAIGAIYKDVATAHVCNDDCPCGWQPQTDLDIEALQRAIAAEAKQQTDLRWLK
                     VAGKA"
     gene            complement(4178..4369)
                     /locus_tag="VGB93_05270"
     CDS             complement(4178..4369)
                     /locus_tag="VGB93_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145555.1"
                     /translation="MPNAAALAALDQEIADVRENIRDLTEQAAAYSGAEDEALTSDRL
                     AEQEALLARLLKDREAAAN"
BASE COUNT          926 a         1226 c         1382 g          967 t
ORIGIN      
        1 gcaagatgtt cccggcgtca ggatggagat ggtccaggcg atcgaatatc gcgaccgcat
       61 aatcgccgcc cgggtcggcg acgggcgccg atgtcttgat cctgagactg gctgtcagca
      121 ggcgcgcgac gagattgccg gctgtcacgc caagccgccg cggcacataa aagcacatcc
      181 gattgcgcgc gaaggcgaca ggcggcaaaa gagcccggcc ttccgccgcg agtctgcgcg
      241 gtgcattcat atcggcggac aagaagagat ctggctcttc gccggcctca atctgggccc
      301 tcagcagccc ggcgctcttg aaggttggct tgaaatcgag cccggcaaga gatgggtcgt
      361 tcgccttagc gagcaagcta acaaaggcct tcaacgagcc ggcggcatag atcgtcactg
      421 tctgcgcggg cgctggattg gccgtggcag ccgtcgcacc gattgcccac agtgccgccc
      481 ctgaaaacgc tccggcccaa cgaatgaaca tctagaaatt cacctcaacg ctcgcctcga
      541 acgttcttgg cgtgccgagc caggcagtgt tgccaccatt ggcaccgacg atagaggcgc
      601 catcggcgat tgaggcataa tagaatttgt cggttacgtt gacgacctgg aaacgcgctg
      661 ttgtgcgatg gccgtaaatg acggtcgaat agcgtgcgcc aagatcgagt gtcgcatagg
      721 ggtcggcgaa ggtcgtgttg gtattgtttg ctgcgcgaga gctttcgtag tgaatcgttc
      781 cggtgacggc gaagccacgc caatagtccg ggtgcagatc aatggcgatg tcgcttttcc
      841 aatgtggcac accgataacg agcatgtcgt tcgtcgcaac gttgccggtg ttgaccagcc
      901 gcgcgtcgat ataggtcacg ccgccgaacg cgcttatgtc cggggtgata tcaccctgtg
      961 cgaaaacctc gccgccccag ttggtttggg tgcctgttac ttcgaaaatg ctcgtggtcg
     1021 gccccgttac gacgctttcc gcgagggggc gcgtcatccg gaagccgtcg accgtgacga
     1081 ggaagtttgg attgaaggca tatttggcgc cgagttcgta catcgtatcg tgatagggcg
     1141 acaggaacac gcttggattg gtgacgcccg aagtcggcgc ctgatcaccg agttcggtgc
     1201 tgttggccca ggtgaaatag gtcgtgagcg gtgagatggg attatagatc aggctaatcg
     1261 tcgggctgag agtcgcgttg gcgcccgtcg aactcgtggt tgcgcctgtc ggattaaagc
     1321 tcgacgagtg gatccatgct gtactcaata ccgcctggag cgcccactgc cgattgaaat
     1381 ggagtgtatc cccggtgatg atcgactgtg agaaaatctt tcccgcttca tattccggcc
     1441 cgtgcgccgg gaagcttgga atcgaggtaa gcactgtcgg cgcaaagagg ttgcccgtgc
     1501 cgatcgtcgg cgtcgcaact gccggattgt aattgtactg accgttgatg aaaccgttgg
     1561 tgccaagcgc aacatcgttt tccatcccat agagccagac acggccgttg agataggcac
     1621 tgttgctgcc gacggtgaag cgcggaacgg cggtaaagtt ctttacgacg ttgtaattgc
     1681 cgctgttgtc ttgcaggata tcagtaatgc cgaagaggcc acgattggca ttctcgtaaa
     1741 ggccgccggc ctcgaagttc cagttctgat tgatctcgtg ccgaattttg agcatgcccg
     1801 tatcgctgac gaggttgtcg ccagcacccg gctgtcctaa gcccggttgc gtgggattaa
     1861 ccgcctgcgg caaaaacgtg ctgtggttcg ttttactcgt ctggctgctg ccgaagacga
     1921 tttcgccggg catgccggtg atgtccgtcg cgtaatggcc gtaatagcct tcgacgtcgg
     1981 tattattgtc gaaacgatag tcgagatcga tactgccgag cgtgcgatct gtcttgctgt
     2041 catcgacata accttcgccg gcgccatgga caatattgac gcggtagcca aggcgaccgt
     2101 cttggccgac gcggccaccg gcatcgatct gctcggtgaa gatgctctgt gaatcgtaat
     2161 cttctcgata ggtgaagaag ctctgatcgg tcggatgctt gagaagatag ttgaacacgc
     2221 cggacgggga ttctggtccg tagagcgcgc cggcgaggcc gttcaaaacc tcaacaccgc
     2281 ggaggttttc gcaatcaatg gccgatgtgc cgatataatt gagaccgtcg agacggacgt
     2341 tctggacgat cgagccttca aagccgcgcg actgcgggcg cgaaacttcg gaaccttgct
     2401 gatcgcgaac ctcgaccgat ggtaaatagc gtaggcagtc attgacggtt tgtgcctccg
     2461 cattctcgat caatgactcg ggaacgatgg tcacggattg gggcgtatcg gccacgctct
     2521 gtccgctgcc caacgggccg agatcggccg gtggtggctg gtagggcgcg cgagaagcat
     2581 ccgctggaag gggattggcg acattcgttg gttgagcgcc ttgaacatta atcgtcggca
     2641 acgcggaaga cgctgtcgcc tgctgggcgt gagcggagca attcacgaaa ccaagcaaag
     2701 ccgagatcag gctaatatct acgaaacgcg caaaaattct ctgtgcgcgg ccacgcggcg
     2761 cgcacatctg atttgacagc atttgggatg gccccccggc cgttgcgttc tgagattttg
     2821 caggcttatg caagccgcac agcgcacaag ctagctatat actcacgggt atagcaatta
     2881 aaatattctt taaagctaga agcttagctc aatgctgacc cgcgctctcc tcatatcggg
     2941 gatggcgagc ggatcgaatc gcgctattct tgaaggctcg aattgggaag atcacgtgcc
     3001 gcaactcgct gaaggtttgc gtatctacgc cattggcgac gtgcatggcc gcgccgacct
     3061 gttggagcgt cttttcgcct tgatcgatgc cgatatcgag caacgcccgg tggagcgcgc
     3121 gattcaggtc atgctcggtg attatgtcga tcgcgggccc gcgtcgcgcg aagtgatcga
     3181 cctcattctg gcgcgggcgg aacggcacga gcttgttgcg ctcaagggca atcatgatgc
     3241 gctcttgctt caggcgatcg atgatcctgc tgcaatgggt gactggctca tgatgtacgg
     3301 aatcgagacc ttggcctcct atgggctgac ctcggcgacc gtcgctggca gccggctcag
     3361 cgatctcgga cgcgcctttg cggcggcgct gccggaagcc caccggaaat ttttccgtga
     3421 gctgaagctt tcttacagcc acggcgattt cttcttcgtt catgccgggg tgcggcccgg
     3481 catcgatctc tcgcttcagg cggaagagga tctcatctgg atacggcagg aatttctgcg
     3541 ctacggcggg gatttcggca aaattgtcgt ccacgggcac agccctgtcc gcgacgtcga
     3601 gcgccatgcg aatcggattg ccatcgacac cgcggcttat gcatctggga agcttaccgc
     3661 tatcgtgatt gaaggcagcg acctgcggat cattgatacg gcagaagccg atgcccacgc
     3721 ggcatgacgg cagtttcaaa ttattctatt tatcaaataa aatcatagac ttgtggatat
     3781 tacgcaaggc tcttattcgt tgatgcgggg cgcggcgacg gagcaaatca tgcaatttaa
     3841 aggcgaatac aaagacgttc tagcgacacg ggagacgatg gcgcgtgaga agatgcgcca
     3901 ggcggtgcta tgcgccttct gccatgtcgt gcagacgaca ggcttgaaac cgatggccgt
     3961 gctgggcctt gccgcgtccg ctattggggc gatctataag gacgtcgcta cggcgcacgt
     4021 atgcaacgac gattgtccgt gtggctggca gccacagaca gatctggata tcgaggcact
     4081 ccaacgcgcc attgcagcag aagcgaagca gcaaaccgat ctgcgttggc tcaaagtggc
     4141 cggcaaggcc tagggttagg gtagggcatc cgctgctcta attcgccgcg gcctcgcggt
     4201 cttttaaaag ccgtgcaagc aaagcctctt gctcagccag acggtcggag gtgagcgcct
     4261 cgtcctcggc gccggaataa gcggcggcct gttccgtcag atcacgaata ttttcacgaa
     4321 cgtcggcaat ttcctgatcc agcgcggcaa gcgcggcagc attgggcatt ggaactcctt
     4381 gtgggcctct tcgaacagcc gggctggacc ctacggcgat ttttacgcaa ggcaagagcc
     4441 cgctctccac ggagaagaat atcctccgga ggccccactg ccgtcaatgt tgttgccgat
     4501 a
//
LOCUS       DASWOG010000259         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_317896,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000259 DASWOG010000000
VERSION     DASWOG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317896"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /gene="pyk"
                     /locus_tag="VGB93_05275"
     CDS             complement(<1..968)
                     /gene="pyk"
                     /locus_tag="VGB93_05275"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0145556.1"
                     /translation="MRRLRRCKIVATLGPATAQRSAIGDLFRAGADVFRINMSHASHD
                     DMRERVLAIRSLEQEFGRPIAILLDLQGPKLRVGLFKDGAVELKRGDIFVFDSDPTPG
                     DASRVHLPHPEILEALRPGHNMLVDDGKVRLHITEASPKRAVAVVDIAGRVSNRKGVS
                     LPDTEIPLASMTPKDHADLLAGLEAGVDWVAVSFVQRAEDVVEVKKIVGSRALVLAKI
                     EKPQAITRLEEIMEASDAFMVARGDLGVEMPLEKVPGLQKRITRIARRLGKPVIVATQ
                     MLESMIALPVPTRAEVSDVAAALFEGADAIMLSAESASGRYPIEAVA"
     gene            complement(1131..1580)
                     /locus_tag="VGB93_05280"
     CDS             complement(1131..1580)
                     /locus_tag="VGB93_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEY0145557.1"
                     /translation="MSPSRKIFRRGLTLLLFGSAALGATPVRADFRLCNMSENRVSVA
                     IAYTDGSHWVSEGWWNLKAGACDNLVRGSLASEYYYVYAMDERGAEWMGKAFMCTADH
                     EFRITGRQDCFVRGFDRTGFFEVDTGRDARNWTVQLTDPTNPGHRGP"
     gene            1776..>2545
                     /locus_tag="VGB93_05285"
     CDS             1776..>2545
                     /locus_tag="VGB93_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0145558.1"
                     /translation="MAKELSSDGFEPVERIEGRADAGVLLLCDHASNALPQSYGTLGM
                     ASADLAAHIGYDIGAADLTRRLAAQLGAPALLTRFSRLLIDPNRGAKDPTLVMRLSDR
                     RLIPGNARIDAAEIGRRRALYWQPYRAAIDDMIAKLSARGPVPAVISVHTFTPVWHGT
                     PRPWQIGILWDNDPRLPHPLMAALEARGIVVGDNEPYDGALPGDTLHEHVTARGLAGL
                     LIEVRQDLVATPEAAHDWADLLAEVLSPILTRPEMHEIA"
BASE COUNT          486 a          805 c          792 g          462 t
ORIGIN      
        1 gcgacggctt cgatcggata gcggcccgag gcgctttcag ccgaaagcat gatggcatcc
       61 gcgccctcaa acagcgcggc ggcgacgtcg gaaacttccg cgcgggtcgg caccggcagg
      121 gcgatcatgg attctagcat ctgcgtcgcc acgatgaccg gcttgccgag gcggcgcgca
      181 atgcgtgtga tgcgcttttg caggccgggc accttctcaa gcggcatttc cacgccaaga
      241 tcgccgcgcg cgaccatgaa ggcatcggag gcttccatga tttcttcgag ccgggtgatc
      301 gcctgcggct tctcaatctt ggcgaggacc agggcgcggc tgccgacgat cttcttgacc
      361 tcgaccacat cttcggcgcg ctggacgaag gagacggcca cccaatcgac gccagcttcg
      421 aggcccgcga gcagatcggc gtgatccttc ggcgtcatcg aggcgagtgg gatttccgta
      481 tcgggaaggc tcacgccctt gcggttggag acgcgcccgg cgatgtcgac cacagcaaca
      541 gcccgcttcg gcgaagcttc ggtgatgtgc aaacgcacct taccgtcatc gaccagcatg
      601 ttatggccgg gccgcagcgc ttccagaatt tcgggatgcg gaagatgcac gcggctcgcg
      661 tcgccgggtg tcggatcgga atcgaagaca aagatatcgc cgcgcttcaa ttcgacagcg
      721 ccgtccttga acaggccgac gcgcagcttc ggtccctgca gatcgagcag gatggcaatc
      781 gggcggccga attcctgctc gagcgaacga atggccagca cgcgctcgcg catgtcgtca
      841 tggctcgcat gactcatatt gatgcgaaac acgtcggcgc cggcgcgaaa taaatcgcca
      901 atggcggagc gttgggctgt tgccggaccc agggtcgcga cgatcttgca gcggcgcagc
      961 cgtcgcattt gagtctcctt caacaatttc aagcgacgtt cgacaggcga tctttgcggc
     1021 tttaatacga aatccccgca taaatcatcc cttagatgaa attttgtgcg ctttggtgaa
     1081 gccaacggga aataaactgc tgtcactgag caaggcgcgg ccgcagcgga tcacgggccc
     1141 cggtgtccgg gattcgtcgg atcggtcaat tgcacggtcc agttgcgggc gtcccgtccg
     1201 gtatcaacct cgaagaagcc ggtccggtcg aaaccgcgaa cgaagcaatc ctgccggccg
     1261 gtgatacgaa actcgtggtc ggccgtacac atgaaggcct tacccatcca ttctgcgcca
     1321 cgctcgtcca tcgcatagac atagtaatat tccgacgcta gcgagccgcg gacgagattg
     1381 tcgcatgccc cagccttcaa attccaccag ccctcgctca cccaatggct gccgtcggtg
     1441 taggcgatcg cgacgctaac gcgattctcg ctcatgttgc agaggcggaa gtcggcgcgt
     1501 acgggcgtgg ccccgagcgc ggccgacccg aagagcagaa gcgtcaggcc gcgcctgaat
     1561 attttgcgtg atggactcat gcggagggtt ctagaccaag gcgaaaactt tgacgcaaca
     1621 atggccatct cgggaggcgc gccagtcgag ccagaatagc gggcctgcgc gcccggcagg
     1681 caagcgaaat ctggctgcgg tgccgcttca tccaaagatc gatccagtga tgtgtggaga
     1741 ttgactccat gcgattcgcc ctttgaaaat agctcatggc gaaagaattg agcagcgacg
     1801 gtttcgagcc ggtcgaacgg atcgaaggac gcgccgatgc aggtgtcttg ctgctctgcg
     1861 atcatgcgag caacgcactg ccgcaaagct acggcacgct tgggatggct tcagccgacc
     1921 tggcggccca catcggctac gacatcggcg ctgctgatct tacccgaagg ctcgcggcgc
     1981 agcttggcgc gccggccctt ttgacgcgct tttcgcgcct gctgatcgac cccaatcgcg
     2041 gcgcgaaaga tccgacgctg gtcatgcggc tttcagatcg ccgcctgata cccggcaatg
     2101 cccgcatcga cgccgcggaa atcgggcgcc gccgcgcact ttactggcag ccctatcgcg
     2161 ccgccatcga cgacatgatt gccaagctct cggcgcgggg ccccgttccg gcggtgattt
     2221 cggtgcacac gttcaccccc gtctggcacg gcacgccgcg gccctggcag attggcattc
     2281 tctgggacaa cgatccacgt ttgccgcatc cgctgatggc ggcgctcgag gcgcgcggca
     2341 ttgtcgtggg cgacaacgag ccttacgacg gcgcgctgcc gggcgatacg ctgcacgagc
     2401 atgtcaccgc ccgcggcctt gcagggctcc tcatcgaagt ccggcaggat ctcgtcgcga
     2461 cccccgaggc agcgcacgat tgggccgatc ttctggccga agtgctgtcg ccgatcctga
     2521 cacggccgga gatgcacgag atcgc
//
LOCUS       DASWOG010000260         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_97936,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000260 DASWOG010000000
VERSION     DASWOG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97936"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VGB93_05290"
     CDS             <1..656
                     /locus_tag="VGB93_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145559.1"
                     /translation="ATVGTPATGIVGAHNDTTGWGAGGGFTFGLLPHWVDVQGSAMIG
                     RGIGRYGSSQLQAATFNPNGSLDALPELMFLGGAVVHATPALDIYAYGGEERILSSDF
                     TPGVTGISFGSPTADNSGCGIIGGTCTGNVRDAWEITGGFWDKVYQGSFGSVRVGLQY
                     SYVQDGLFSGSGHTQSALPGGLAAGSSVHYSDNQVYASFRYYPFDPPPAAPPVIAKY"
     gene            926..1642
                     /locus_tag="VGB93_05295"
     CDS             926..1642
                     /locus_tag="VGB93_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145560.1"
                     /translation="MMTMTAGKFEPEDCPAVLLIEDEFETAQEVSLFLERQGYAVRIA
                     DSIQQVPDAIRLHAPAVMILDRMLHGTDVLPMIEAWREQGNHVPVLVVSGLSSVDDRI
                     RGLRAGGDDYLIKPFSLEELIARVEVLRRRTADARISVLRVGPLTMDLIERDVRRADR
                     ELDLLPREFELLEYFMRHPNLVITRAMLLTDVWNFKTLPQTNVVDVHIGKLRRKIDGP
                     GEVALIETVRATGFMLHGDR"
     gene            1629..>3034
                     /locus_tag="VGB93_05300"
     CDS             1629..>3034
                     /locus_tag="VGB93_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0145561.1"
                     /translation="MGIGELLRSAAFRLAVAFAVVVTLSTTIVFGFVYWHVANADMMR
                     ARISLADEVTTASVEPDAQLMPELQRRLTRDLRQIEYVGLFDANGKHIYGNVQTLPPI
                     PLDGVYHTIWTTSLDGTGQESAFFVGRPRPGGGALVLGRSLYEIHALQQSVVRALAIG
                     IGPTILVALLAGIVLSLRATRRLNAIHLAIDRVIEGNLHERLPSHRIPDGIDEVVRSV
                     NRMLDEITRLMNQLKQVGDNIAHDLRTPLTVAHIRLQRALDEPEDQLRATTRQIVDDL
                     NRAVTTVTALLRISEIESGLQRGAFAQIDLTKLCRDVFDFYAPLAEAKSIKMTLRAQN
                     KVPLVGDADLLREALMNLLDNAIKFTPRGGIVQMAVENSAQGPRVEIRDNGPGVLPSE
                     RDRIFKRFFRSAATDHTPGNGLGLSMAATIVEMHGLSLRLGEATDGACFEISASQKST
                     VENRKPSHEIERRTSLHNA"
BASE COUNT          586 a          880 c          888 g          680 t
ORIGIN      
        1 atgcgacggt cggaacgcca gcgaccggaa ttgtgggcgc ccacaacgac acgaccggct
       61 ggggtgctgg cggcggcttc accttcggcc tccttccgca ttgggtcgat gtgcagggtt
      121 ctgccatgat tggccgcggt atcggccgct acggttcttc gcaacttcag gctgcgacgt
      181 tcaacccgaa cggctcgctc gacgctctgc cggaactcat gttcttgggt ggcgctgtcg
      241 tccacgcgac tccggccctc gatatctacg cttacggtgg cgaagagcgt atcctgtcct
      301 ccgactttac gccgggcgtg acgggcatct cgtttggcag cccaacggct gacaattcgg
      361 gttgtggtat catcggcgga acctgcaccg gcaacgtccg tgacgcttgg gaaatcaccg
      421 gcggcttctg ggacaaggtc taccagggct cttttggttc ggttcgcgtc ggcctgcaat
      481 attcctacgt gcaggacggt ctcttctccg gatcgggtca cacgcagagc gccttgcccg
      541 gtggcttagc ggctggcagc tcggtccact acagcgacaa ccaagtttac gcctcgttcc
      601 gctactatcc gttcgatccg ccgccggcgg ctccgccagt catcgcgaaa tactgatcgc
      661 gatacgcaac acagacatgc taaaagcggg ctccggcccg cttttttcat ttaaccttgc
      721 cctgctaaat ctctgattat gaggcaggaa cgggcgtctg gatcaacaga gcgtgtccgg
      781 caaatattaa atgctttttt atgtacttgg ctgatttgtc tcagtatttt ttggcctcct
      841 tgggcggttt taatgtaatt tgcggcctcc tttccgaccg cggcgacacc ggggaagcgg
      901 agcaaatttc gagatgttta gcgagatgat gacgatgact gccggcaaat tcgaacctga
      961 agattgtccg gcggtcttgc tgatcgagga cgagttcgaa acagcgcagg aagtctcgct
     1021 cttcctcgag cggcaaggat atgccgttcg aatcgccgat tcgatacagc aggttccaga
     1081 tgcgatccgg ctgcatgcgc cggccgtcat gattctcgac cgcatgttgc atgggaccga
     1141 cgttctgccg atgatcgaag cttggcgcga gcagggcaac catgtgccgg tgctcgtcgt
     1201 cagtggactt tcctcggttg acgatcgcat ccgtggcctg agagcgggtg gcgacgatta
     1261 tctcatcaag cctttctcgc tcgaagaatt gatcgcccgg gtcgaagtct tgcgacggcg
     1321 cacggcagat gcgcgcatat ccgtgttgcg cgtggggccg ctgaccatgg atttgatcga
     1381 acgtgacgtc cgccgtgccg acagggaact tgacctcctg ccgcgcgagt tcgagcttct
     1441 cgaatatttt atgcgccatc cgaatctggt catcacccgc gccatgctgc tgaccgatgt
     1501 ctggaacttt aaaacattgc cgcaaaccaa tgttgtcgac gtgcacatcg gcaagctgcg
     1561 tcgcaaaatc gatggccccg gcgaagtcgc gttgatcgag acggtccgtg cgacaggatt
     1621 tatgctgcat ggggatcggt gaacttcttc gatcggcggc ttttcgcctc gccgttgctt
     1681 ttgcggtcgt cgtcacgctt tcgacgacga ttgttttcgg attcgtttat tggcacgtcg
     1741 ccaatgccga catgatgcga gcgcggatat cgcttgccga tgaagtcact acggcctcag
     1801 tcgagcccga tgcgcagctg atgcccgagc ttcagcggcg cctgacgcgt gacctacgcc
     1861 aaatcgaata tgtcggcctc ttcgatgcga acggcaagca tatctatggc aacgtgcaga
     1921 cattgccgcc aatcccgctt gatggtgttt atcatacgat ctggacgacg tcgctcgatg
     1981 ggactggtca ggaatcggcg ttcttcgtcg ggcgcccacg gcccggcggc ggtgccctgg
     2041 tgttggggcg cagcctctat gagattcatg cgcttcagca atccgtcgtg cgcgccttgg
     2101 cgatcggcat cggtccgacg atcctcgtcg cgctgcttgc cggtatcgtt ctgagcctgc
     2161 gtgccacgcg gcggctcaac gctattcatc ttgccatcga ccgggtgatc gaaggcaatc
     2221 tgcacgaacg tctgccatcg catcgaatcc cggatgggat tgatgaggtg gtgcgctcgg
     2281 tcaaccgaat gcttgacgaa atcacccgcc tcatgaatca gctcaagcaa gtcggcgaca
     2341 atattgcgca tgatctgcgc acgccattga cggtcgcaca tatcaggctt cagcgagcgc
     2401 ttgacgaacc agaagaccaa ttgcgtgcca cgacgcggca aatcgttgat gatctcaatc
     2461 gcgcagtgac gacggtgacg gcgctgctgc gcatctccga aatcgaaagc gggctccagc
     2521 gcggcgcctt tgcgcagatc gacctgacca aactttgccg cgacgttttc gatttttatg
     2581 cgccgctcgc ggaggccaag tcgatcaaga tgacactccg tgctcaaaat aaagttcctc
     2641 ttgtcggcga cgccgatctc ctccgtgaag cgctgatgaa ccttctcgat aatgccatta
     2701 agttcacgcc gcgggggggc attgtacaga tggcggtcga aaatagcgct cagggaccgc
     2761 gcgttgaaat tcgcgacaac gggccgggcg ttttgcccag cgagcgcgac aggattttca
     2821 aaagattctt tcgctcggcc gccaccgacc atacgccggg caatgggctc gggctgagca
     2881 tggccgcgac aatcgtggaa atgcacgggt tatcgcttag gcttggcgaa gccacggacg
     2941 gtgcgtgttt cgaaatttcc gcttcacaaa agagcactgt cgaaaacagg aagccaagcc
     3001 acgagataga gcgacggaca tccttgcata acgc
//
LOCUS       DASWOG010000261         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_97947,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000261 DASWOG010000000
VERSION     DASWOG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97947"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1615
                     /locus_tag="VGB93_05305"
     CDS             110..1615
                     /locus_tag="VGB93_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0145562.1"
                     /translation="MRDFVTVNQQPYSNNRSGGIMQGLQGRRPRFGLATRIVISVVVF
                     VLTAEFMIYVPLIAHYRDNWLRNRLSAAYTATLVLEAAPRAMVSPQLSRELLDSVGAR
                     IIVLKAHGTRRILAATTLPPAVDEIYDLRHWSFLQSLASAWRTYFAADNRVITVLGAA
                     PMGGDGIEVTMDEGPAKEALHSSAARALMVSLTISALVSALAVIILHVVVLGPMRRLT
                     TNITEFGADPENASRIIVPSGTTHEIGRAEEALATMQDTLVRELTQKKHLAALGLAVA
                     KINHDLRNMLAAAQLLSDRLGNVTDPLAQRLAPKLVATLDRAIRFCQTTLTYGRATDE
                     APRPRFVDLHNVVAEAAESVCPSCGSRVAIVNNVNVGFVLWADSEQLFRVLMNLMRNG
                     VEALDRAGPARGRSAQIMIAADFAGDDVIIEVADTGPGVPPSVRAQLFTPFSSSSRPG
                     GSGLGLAIAADLVRAHGGSIELAPVDDDADENTGATFRITLPQRRRPAVAA"
     gene            1730..1806
                     /locus_tag="VGB93_05310"
     tRNA            1730..1806
                     /locus_tag="VGB93_05310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1764..1766,aa:Arg,seq:acg)
     gene            2055..>2257
                     /locus_tag="VGB93_05315"
     CDS             2055..>2257
                     /locus_tag="VGB93_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY0145563.1"
                     /translation="MAKRRLVWLLFCVTAVAIFILDAVTSVDMPISVLYVTLIMIVLR
                     SGTARDILIAGMTCCVLTIVSYWT"
BASE COUNT          415 a          712 c          658 g          472 t
ORIGIN      
        1 caatgcgcca gcgctgacgc aatccatctc cgaggctgcg cttgcatcta cgccctatct
       61 cggcaaagct ctggggcagc ggttctttcc gcgctggacg agataggcaa tgcgcgactt
      121 cgttaccgtc aaccagcagc cttattccaa caatcgttct ggcggcatta tgcagggcct
      181 gcaaggacgg cgcccgcgct ttggcctcgc cacgcgaatc gtcatatccg tcgtcgtttt
      241 cgtgttgacg gcggaattta tgatctatgt gccgctgatc gcgcattacc gcgacaattg
      301 gctgcgcaac cgtttaagcg cggcctatac cgcgaccctg gttctcgaag cggcgccgcg
      361 ggcgatggtc tcgccgcaat tgtcgcgcga actgctcgac agcgtcggtg cacgcatcat
      421 tgtcctgaaa gcgcacggta cgcggcgcat tctcgcggcc acgaccctgc ccccggcggt
      481 cgatgagatc tacgacctgc ggcattggtc cttcctgcaa tctcttgctt cggcatggcg
      541 gacctatttt gccgccgaca atcgcgtcat cactgtcctt ggcgccgccc cgatgggcgg
      601 tgacggcatc gaagtgacga tggacgaggg accggcgaaa gaggcgttgc acagcagcgc
      661 agcccgcgct ctgatggtct cgttgacaat atcggcgctt gtctcggcgc tcgcggtcat
      721 catcctccat gtcgtcgtcc taggcccgat gcgccgcctc acgaccaaca tcaccgaatt
      781 cggcgccgat ccggaaaatg ccagccggat tatcgtgccg tcgggcacca cgcatgaaat
      841 cggccgcgcc gaagaggcgc tggcgacgat gcaggacaca ctcgtccgcg agttgacgca
      901 aaagaagcac ctcgccgcgc ttggtctcgc cgtcgccaag atcaaccatg atctgcgcaa
      961 catgctggcc gccgcgcagc ttctctccga tcgcctcggc aatgtcaccg atccgctagc
     1021 gcaacggctg gcgcccaagc tcgtcgcgac gctcgaccgc gccatccgct tctgtcagac
     1081 cacactgacc tatggccgcg cgacagacga ggcgccccgt ccacggtttg tcgatttgca
     1141 caatgtcgtc gcggaggcag ccgaatcggt ttgtccgagc tgcggcagcc gcgtcgcaat
     1201 tgttaacaac gtcaacgtcg gtttcgtgct ctgggccgat tcggagcaat tgttccgggt
     1261 gcttatgaat ctgatgcgca acggcgtgga ggcgctcgac cgcgccggtc cggcgcgtgg
     1321 ccggtcggcg caaatcatga tcgccgcaga cttcgccggc gacgacgtga tcattgaagt
     1381 cgccgatacg ggccccggcg tgccgccgag cgtgcgcgcg cagctcttca cacctttttc
     1441 gagctcgtcg cgcccaggcg gctcggggct gggactcgcc atcgcggccg atcttgttcg
     1501 cgcgcacggc ggcagcatcg aactggcgcc tgtggatgac gatgcggacg agaataccgg
     1561 cgcgacattc cggatcacat tgccgcagcg tcgccgccct gcagtcgcag cctgaacatc
     1621 aaagcagcct gttgccatcc tccgcccgag ccgctaagga agccgtccat ccttgggatc
     1681 gacctcttcc cgatcatcgg caagttttcg gcttcccttg cggatttgcg cgcccgtagc
     1741 tcagctggat agagcactag actacgaatc taggggtcag gagttcgaat ctcttcgggc
     1801 gcgccattct gcactcatcc gcgaacctgg gtccaagtct gttcggctgg ccagaacagc
     1861 cttttccgga acgtccttac gccctttgat gcggatgtac gtgtcctcga cctcgatgac
     1921 gtcgatcgga agtaagcttt tcggaacggc acagcgtgca aaaaaatgac ctcgcccgag
     1981 gccctgaggt aaggtagctc gagcgatagg tacagattag caagttaggt atatagaatg
     2041 gcggagactt aaaaatggcg aagcgtcgtc ttgtctggct cctattctgc gtaacagcgg
     2101 tcgcaatatt tattttagac gccgtcacaa gcgtcgatat gccgatttct gtcctgtacg
     2161 tgacgttgat catgatcgtt ctgcgatccg gcacggccag ggatatttta atcgccggca
     2221 tgacgtgttg cgttttgaca atcgtcagct attggac
//
LOCUS       DASWOG010000262         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_79190,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000262 DASWOG010000000
VERSION     DASWOG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79190"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VGB93_05320"
     CDS             complement(<1..331)
                     /locus_tag="VGB93_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004127338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0145564.1"
                     /translation="MGELTGKVAIVTGASKGIGAGIAKHLAAAGASVAVNYASSREGA
                     DKVVAEITGKGGKAIAVHGDVAKKADVKKIFETALASYGRLDILVNNAGVFKFDPIET
                     VTETEFHR"
     gene            425..1336
                     /locus_tag="VGB93_05325"
     CDS             425..1336
                     /locus_tag="VGB93_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0145565.1"
                     /translation="MSDRLSALRLFTRVARTGSFSQAGREFQLSQPSASRIVAELERE
                     VGVSLFTRTTRAVTLTEAGAEYLARVEPILAELEEADHVARGTGEIRGLLRVGLSSSF
                     AVRAVIPLLPPFMACHAELRIDLLMNDQRQDLLGDGVDVALRFGPLPDTSATARRIGS
                     VPRLLAASTAYLERAGVPKTPDDLADHAIILGPAGLDPGAWTLRRDNHVVTARVEGRL
                     RISANEGAIAAAVAGLGIVSTGWWGCLAELQSGTLVRVLTDWEMETADVHAVFPAGRA
                     TKPAARAFVDYLMRELDKGTAAHTVCM"
     gene            complement(1371..1727)
                     /locus_tag="VGB93_05330"
     CDS             complement(1371..1727)
                     /locus_tag="VGB93_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY0145566.1"
                     /translation="MTITIYGIKNCDTMKKARAWLEGHGVEYAFHDYKAVGIDRAHLE
                     KWVKKTGWETLLNRAGTTFRKLPAADKTDLDEHKAIALMLEYPSAIKRPVLELGGGKI
                     LVGFKPEAYENARLGG"
     gene            complement(1795..2709)
                     /locus_tag="VGB93_05335"
     CDS             complement(1795..2709)
                     /locus_tag="VGB93_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0145567.1"
                     /translation="MELRHLKFFVAVAEEGSLTTAAEKRLHTAQPSLSRQMRDLEYEV
                     GAQLFVRSAKGVELTPAGRAFLDHARLALAQVEAATIAARRAAEPAKASFALGFLTGQ
                     EMDWLSEAMHILRDELPNLNVTVSSQYSPLLADALMRGKLDLAFLRPEPNLPDIAYKV
                     VTTEPLVVVMPSDHRLATRKVIAVQDIARETFLGMSKTAPVLQTIMADYFERVGLDIR
                     ASHEIDNLGMAMSLVASTRGVTLLPAYAQNFLPWSVVARPLAGEAPTIDLVMGYHKAN
                     TSPILKLFLSRSDDLIARVRKKLRAAAN"
     gene            2852..>3104
                     /locus_tag="VGB93_05340"
     CDS             2852..>3104
                     /locus_tag="VGB93_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0145568.1"
                     /translation="MSQVVLITGGLTGIGRATAVAFAFAKEGAKVVVSGRHEKEGHAL
                     VDELKGLGADAAFVKTDVRNDAEVARLVDETIARFSRLDI"
BASE COUNT          562 a          969 c          927 g          646 t
ORIGIN      
        1 cgcgatgaaa ctcggtttcc gttacggtct ctatcgggtc gaatttgaac acgcccgcgt
       61 tattgacaag gatgtcgagg cggccgtagc tcgcaagcgc cgtctcgaaa atctttttga
      121 cgtcggcttt cttggctacg tcgccgtgga cagctattgc cttgccacct ttgccggtaa
      181 tttccgcgac aaccttgtcc gccccttcgc ggcttgatgc ataattcacc gcgacagaag
      241 cgccggccgc agcgagatgt ttcgcgatcc ccgcgccgat gcccttggac gccccggtaa
      301 caatcgcgac cttgcctgtg agttcaccca tatgaccctc ctggttggta gtcggggctc
      361 aaaatggcta ttcgtccgtc agggggtagc gcatctcgcc aactattata tatttgtaat
      421 tcaaatgagt gaccggcttt ccgccctgcg cctttttacc cgcgtcgcgc gaacgggcag
      481 tttttcccag gcggggcgcg agttccagct ctcgcaaccc tctgcctcgc gcatcgtcgc
      541 ggaattggaa cgcgaagttg gcgtgtcgct cttcacgcgc acgacacgcg cggtcaccct
      601 gaccgaggcc ggcgccgaat atctggcgcg cgtagagccg atccttgctg agctagaaga
      661 ggcggatcac gtcgcgcgcg gcaccggtga aatccgtggc ctcttgcgcg tcggcctctc
      721 gtcgagcttc gcagtgcgcg ccgtcattcc gcttttgcca ccattcatgg cctgccacgc
      781 cgagctgcgg atcgatcttc tcatgaatga tcagcgccag gatttactcg gcgacggcgt
      841 cgatgtcgct ctgcgcttcg gcccgcttcc agatacgagc gcgacggcac gacgtatcgg
      901 ttcggttcca cgtctgctgg ccgcgtcaac cgcgtatctc gaacgggccg gcgtgcccaa
      961 gacgccggac gatctcgccg accacgcgat cattctcggg ccggccgggt tggaccccgg
     1021 cgcttggact ttgcggcgcg acaaccacgt tgtaacggcc cgtgtcgaag gtcggctcag
     1081 gatttccgcg aatgaaggcg cgattgccgc cgccgtcgcc ggtctcggaa tcgtttcgac
     1141 cggctggtgg gggtgccttg ccgagcttca gagcggcact ctggtccgtg tgttgaccga
     1201 ctgggagatg gagacggcgg acgtccacgc ggttttccca gccggccgcg caacaaagcc
     1261 cgccgcgcgc gccttcgtcg attatctgat gcgggaactc gacaaaggta cggcggccca
     1321 cacggtctgt atgtgacgtc aaatgttcat cggccgctcg ccgatgttgg tcagccgccc
     1381 aatctcgcgt tctcataggc ctcgggtttg aagccgacga ggatcttgcc accgccgagt
     1441 tcgagcacgg gacgtttgat ggccgacgga tattcgagca tcagcgcaat agctttatgc
     1501 tcatcaaggt ccgtcttatc ggcggcaggg agtttgcgga aggtcgtgcc ggcacgattg
     1561 agcaaagtct cccagccggt tttcttcacc cacttttcga gatgcgcgcg atcgatgcct
     1621 acggccttgt aatcgtggaa agcgtattcg acgccgtgcc cttcaagcca ggcgcgtgcc
     1681 ttcttcattg tgtcgcagtt tttgatgccg tagatcgtga tcgtcatcgc gaagaatcca
     1741 gccgtttgtc aggcggcgtt taatagcgtt tgctcctccg aagtccaaac ggctttaatt
     1801 cgccgccgcg cgcagcttct tcctcacgcg tgcgatcaaa tcgtcgctgc gagacaggaa
     1861 cagtttgagg atcggcgatg tgttcgcctt gtgatagccc atgacgagat cgatggtcgg
     1921 cgcttcacct gccagcggcc gggcgacaac ggaccagggc aagaagttct gcgcataggc
     1981 tggtagcagt gtcacgccgc gcgtcgaggc gacgagcgac atcgccatgc cgagattgtc
     2041 gatctcgtgg ctcgcgcgaa tgtcgagacc gacgcgctca aaataatcgg ccatgatcgt
     2101 ctgcaaaacc ggcgcggttt tcgacatgcc gagaaacgtc tcccgcgcga tatcctggac
     2161 cgcgatcact ttgcgcgtcg ccaacctgtg gtcgcttggc attacaacga cgagaggctc
     2221 ggtcgtcacc accttatagg cgatgtccgg aaggttcggc tccggccgca gaaaggcgag
     2281 atcgagcttg ccgcgcatca gggcgtcggc aagcagcggc gaatattggc tcgatacagt
     2341 gacattgagg ttcggcaatt cgtcgcgcag aatgtgcatg gcctcggaca gccaatccat
     2401 ctcctgcccg gtgaggaagc cgagtgcaaa gctcgccttg gcgggttcag ctgcccggcg
     2461 cgcggcaata gtggcggcct cgacctgcgc cagcgccagc cgcgcgtgat caagaaaagc
     2521 acgacctgcc ggcgttagct cgacgccctt cgcgctgcgc acgaagagct gcgcaccaac
     2581 ttcatattcg aggtcgcgca tctggcggct gagcgaaggt tgcgccgtgt gcagacgctt
     2641 ttccgccgcc gtcgtcagac tcccctcctc cgccacggcg acgaagaact ttagatggcg
     2701 taattccatt agccatacct cgaaagcatg gcccgcatcc tacaaagtct tttccatcgc
     2761 accaggagag cctatctgac cgtcatgcgg gagcgcaccg ctctagcagt cataccccgc
     2821 cggtaggcgg ggcacttacg aggacaaagc catgagccaa gttgttctaa tcactggcgg
     2881 cctcaccggc atcggtcgtg ccaccgccgt cgccttcgcc ttcgccaagg aaggcgccaa
     2941 agtcgtcgtc tcgggccgtc acgaaaaaga gggtcacgcc ctcgtcgacg aattgaaagg
     3001 tctcggcgcg gacgcggcgt ttgtcaagac cgatgtccgc aacgatgcgg aagttgcgcg
     3061 ccttgtcgac gagacgattg ctcgcttcag ccggctcgac attg
//
LOCUS       DASWOG010000263         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_134611,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000263 DASWOG010000000
VERSION     DASWOG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134611"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGB93_05345"
     CDS             complement(<1..297)
                     /locus_tag="VGB93_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145569.1"
                     /translation="MKARAALSFDIAPKASTRAPIETHPSIFATALHESGVGLCLIAI
                     ALWALTHPFQGIAGDTNVYIGRALADLNPNGIGRDRMFVDDGQSRFSLFPFLLDH"
     gene            complement(294..758)
                     /locus_tag="VGB93_05350"
     CDS             complement(294..758)
                     /locus_tag="VGB93_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0145570.1"
                     /translation="MSVVDSVSHVERGAPLAPSKSLRVLIADLFGYGLVSAAALGLDW
                     GLLITFVKAGLNYQVASAMSFTLGMLLAYAGSVLLIFRGRRQRSIGAEAVGFFAIGFL
                     GLALNQALLFAFVHFGGLGVGLAKAPTAGFVFTFNFFARRTLLFVSEAAAPK"
     gene            complement(760..>1571)
                     /locus_tag="VGB93_05355"
     CDS             complement(760..>1571)
                     /locus_tag="VGB93_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145571.1"
                     /translation="ALGGEIVMDRKLRSLSYDKHTGNWSVAVESSNGQVEHYTASHVV
                     SSAPVRELVSALKPTALTLLHARELKYRDFLTVVLIGETKSELPDNWIYIHDPKVKVG
                     RVQNFRSWSPEMLPDETSTCLGLEYFCFEGDGLWNSTDANLIARAKGEIGEIGLMDPA
                     DVRDACVVRQKKAYPVYDETYADHMKEIRRELRLSCPNLHLVGRNGMHKYNNQDHAMM
                     TGMLTAANIIAGEELYDVWQVNEDAEYGEAGASGAQEALKSLRLVPKRVAA"
BASE COUNT          327 a          464 c          495 g          285 t
ORIGIN      
        1 atggtcaagc agaaacggaa acaggctgaa acgcgattgt ccgtcatcga cgaacatcct
       61 gtcacggccg atgccgtttg ggttgagatc ggcgagggcg cggccgatgt acacgttcgt
      121 atcgccggcg atgccttgga acgggtgggt cagcgcccaa agcgcgatgg caataaggca
      181 aagcccaaca ccgctctcat gtagagccgt ggcaaatatc gatgggtgcg tttcaatagg
      241 ggcccgcgtc gacgccttgg gtgcgatatc gaacgaaagg gccgcgcgcg ccttcatttc
      301 ggggcggctg cctccgacac gaacaacagg gtgcgccggg caaagaagtt gaatgtgaag
      361 acgaaccccg cggtcggcgc cttggcgagg ccgacgccga ggccaccgaa atgtacgaag
      421 gcgaagagca gcgcctggtt cagggcgagg ccgagaaagc cgatggcgaa aaaaccgact
      481 gcctccgccc cgatgcttcg ttggcggcgg ccgcgaaaga tcagcagcac gctaccggca
      541 taagcgagca acatgccgag cgtgaagctc atcgccgacg cgacctgata attcaggccg
      601 gctttgacga aggtgatcag gagcccccaa tcgagaccga gcgccgcggc gctcacgagg
      661 ccatagccga aaagatcggc aatgagcaca cgcagcgact tgcttggagc caatggcgcg
      721 ccgcgctcga cgtgcgatac agagtcgacg acgctcatgt caggccgcca cgcgcttcgg
      781 cacgagccgc aagctcttca gcgcctcctg cgcgccggaa gcgccggcct cgccatattc
      841 ggcgtcctca ttcacctgcc agacgtcgta cagctcttca ccggcaatga tgttggcagc
      901 ggtcagcatg ccggtcatca tcgcatggtc ctgattgtta tatttgtgca tgccgttgcg
      961 gccgacgaga tgcaggttgg ggcaggagag ccgcagctcg cgccggattt ccttcatatg
     1021 gtcggcataa gtctcgtcat agacgggata ggccttcttc tgccgcacga cgcaggcgtc
     1081 gcgaacatcg gcagggtcca tcaggccaat ctcgccgatt tcgcctttgg cgcgggcgat
     1141 caggttcgcg tcggtcgaat tccacagacc gtcgccttcg aagcagaaat attcgaggcc
     1201 gaggcaggtc gaagtttcat cgggtagcat ttccggcgac caggaccgga agttctgcac
     1261 gcggccgacc ttcaccttcg gatcgtggat atagatccaa ttgtcgggca actcgctctt
     1321 ggtttcgccg atcaggacga cggtgagaaa atcgcggtat ttgagttcgc gcgcgtggag
     1381 cagcgtcagc gccgtcggct tcagggcgga gaccaattcg cgcaccggtg ccgaagagac
     1441 gacatgcgag gcggtataat gttcaacctg cccgttgctg ctttctacgg cgacagacca
     1501 attgcccgtg tgcttgtcat agctgagcga gcgcagcttt cgatccatga cgatttcgcc
     1561 gccgagcgct t
//
LOCUS       DASWOG010000264         6093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_317972,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000264 DASWOG010000000
VERSION     DASWOG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317972"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..120)
                     /locus_tag="VGB93_05360"
                     /pseudo
     CDS             complement(1..120)
                     /locus_tag="VGB93_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241357.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(183..986)
                     /locus_tag="VGB93_05365"
     CDS             complement(183..986)
                     /locus_tag="VGB93_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0145572.1"
                     /translation="MFEYDFMRNAFAAATVVAIVAGCVGYFLVLRGQTFAGHALAHVG
                     FTGATGAVLVGIAPLWGLVAMTVAAGILMGFMSEKLAQRDVAIGIVLAFSLALGLLFL
                     HFFTSFATHATALLFGNVLGVDLPTVGILVVLGAICLVTLAIVSRPLLFASLQPELAE
                     AKGVSLRLYSVVFLAIVALATAECAQIVGVLLVFALMVGPAATAQRLVSGIGAGVLLS
                     AVLALVEAWGGLTLAFYTDWPTSFWITALSTLIYALAAAPRQIGAWRAA"
     gene            complement(979..1815)
                     /locus_tag="VGB93_05370"
     CDS             complement(979..1815)
                     /locus_tag="VGB93_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145573.1"
                     /translation="MNAIELRDVTLALGGHNVLANISLNIPAGQFIGVLGPNGAGKTT
                     LMRAILGLLSPTNGTIRVLDKPATRGNPAIGYRPQTAAALPALRISGWDFVACVADGF
                     RPGLPILTAETRAQVHRALELVGAEQLARRPLTELSGGERQRLLLAQALIGDPKLLLL
                     DEPLISLDPRRQQEAVELVKRLQTDLGITVLFSAHELNPLLNAIDQVLYLGSGHAALG
                     RVEDVVTPPVLSKLYGAPIDVIRVRDRIFVMSGGHDVERDDHRHEHDHPHSHAHEDHS
                     HV"
     gene            complement(1822..2718)
                     /locus_tag="VGB93_05375"
     CDS             complement(1822..2718)
                     /locus_tag="VGB93_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0145574.1"
                     /translation="MPFPIGAMRAHRQKLFFALAVASAAMSPVGAQAAPVAVVAAENF
                     YGDVAKQIGGDDVSVTSILSNPDEDPHLFEASPSVARAISGAKIVIASGADYDPWVQK
                     LLSASNSADRKSIVVADLVGKKSSDNPHIWYDIRIMTAFAKAFAADLGAIDPSHKAAY
                     DKRLAQFEASLQPIHTKIVTLQARLAGRAVTATEPVFGYMLDALGLTSRNQGFQIAVM
                     NNTEPSASDVAGFEDDLKNHKVVMLVYNSQASDPVAERMEKLAKQSSIPVVGATETEP
                     RGVAYQQWMSSELDAVDHALPK"
     gene            2909..3490
                     /locus_tag="VGB93_05380"
     CDS             2909..3490
                     /locus_tag="VGB93_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0145575.1"
                     /translation="MSRRLFLPFAILVLGFAGVGLGSFLTLSKQAGVPIPSIGGPFRL
                     ASSNGGEATDADFKGHPFLVFFGYTHCPDICPTELFQMSQMLKAAGADKPLKVLFITA
                     DPERDTAPVMKQYLSSFDSRIIGLTGTRAQINAVEKEYRVYARKVPSANGKDYSMDHT
                     AVIYLMDKQGQFVSAFDIERPPQVAAQDLEAYL"
     gene            complement(3497..4354)
                     /locus_tag="VGB93_05385"
     CDS             complement(3497..4354)
                     /locus_tag="VGB93_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0145576.1"
                     /translation="MGILSKTYFRAKRAAGILLLVALTAPTLALGQAATVPGYFDLQN
                     RLEKPDLGSLHTLRFLTDDTYPPFHFALADGTLTGFDIDLARAICEELKIACTIQARR
                     FDLLENALNAGDGDALIASLRIDAASHTRFEFTAPYYTTPARFVARTGATFEATPEGL
                     DGKPVGIIGGTAHEAYLKAFFPHVRREAFANRGELYAALRGGQTDAGVDEAITSSFWL
                     AGKDSNNCCAFRGGPYTDSNYFGEGVGIAVKKGNDDLRRALDYALVDLARRGVYTDIY
                     LKYFPIGFY"
     gene            4459..4842
                     /locus_tag="VGB93_05390"
     CDS             4459..4842
                     /locus_tag="VGB93_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145577.1"
                     /translation="MRLGKSARANPVPARKTSAAKPSEPLALAPSTFSPARGTSHLPS
                     EIGFLAHYGVSFVRLANAVETARRQGVTADRVLIAEGQISEDYFYRALARHLRLTFID
                     ADVALKAAAPYPQMIEAGAVALAPG"
     gene            5420..5920
                     /locus_tag="VGB93_05395"
     CDS             5420..5920
                     /locus_tag="VGB93_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0145578.1"
                     /translation="MDVAPPGAPRTKPRALNVALPLLRGELVAVYDAGDIPDPQQPRL
                     AASRFAAAPARLACLQAQLAIDNIGDGWLPNCLMAQTRVCAIRPLPKLFAIEYAELFD
                     VINTGLSALRIPFPLGGRSNHFRTDVFRRVGGWDAWNVTEDADIGFRLARFGYDSETL
                     ASTTYE"
BASE COUNT         1136 a         1871 c         1896 g         1190 t
ORIGIN      
        1 ttcattgacg atgacggcga atttcttgcc gtgcggttcg ctcaggatac gattgagcag
       61 cgtcgttttg cccgcgcaga gctagccggt gcgcccggtg ccgggcaatt tttcggccat
      121 gccaagctcc ggggaaatga tcgcgggcgc caatctagcg cggatcgtgg ccagaatggg
      181 ggtcaggccg cgcgccaggc gccgatctgc cgcggcgccg cggccagggc atagataagg
      241 gtgctgagcg ccgttatcca gaagctggtc ggccagtccg tgtagaaggc aagtgtcagg
      301 ccgccccagg cttcgacaag tgccaggact gccgaaagaa gcactcctgc gccgatgccg
      361 ctcaccagcc gttgcgctgt cgcggcgggg ccgaccatca gggcgaagac gagcagcacg
      421 ccgacgatct gggcgcattc ggccgtcgcc agcgctacga tggcgagaaa cacgaccgaa
      481 taaagccgca gcgagacgcc cttggcctcg gcaagttccg gttgcaggct ggcaaagagc
      541 agcggccgcg aaacgatcgc aagcgtcacg aggcagatcg cgccgaggac cacaaggatt
      601 ccgaccgtcg gcaaatcgac accgagcaca ttgccgaaca acagcgccgt ggcgtgggtc
      661 gcaaagctcg taaagaaatg cagaaacagg aggcccagcg cgagcgaaaa ggcgagcacg
      721 atgccgatcg cgacatcccg ttgcgctaac ttttcgctca tgaagcccat gaggatgccg
      781 gcggcgaccg tcatcgcgac caggccccag agcggcgcga tgccgacgag cacggcgccc
      841 gtcgcgccgg tgaagccgac atgtgccagc gcatggccag caaaggtctg cccgcgcagc
      901 accaggaaat agccgacgca accggcgacg atcgcgacca ccgtcgccgc agcgaaggcg
      961 ttgcgcataa aatcatattc aaacatgcga atggtcttcg tgggcgtggc tatgcgggtg
     1021 gtcgtgctcg tgccgatggt catcacgctc gacatcgtgg ccgcccgaca tcacgaagat
     1081 acgatcgcgg acccggatga cgtcgatcgg cgccccgtaa agcttcgaca ggaccggcgg
     1141 cgtcaccacg tcttcgaccc ggcccagcgc ggcatggccg ctgcccagat aaagcacctg
     1201 atcgatagcg ttgagcaggg ggttcaattc atgtgcgctg aagagcacgg tgattccgag
     1261 atcggtttgc aaccgcttga ccagttcgac agcctcctgc tggcggcgtg gatcgaggct
     1321 gatcaggggt tcgtcgagga ggagcaattt ggggtcgccg atcagcgcct gtgccaataa
     1381 caggcgctga cgctcgccgc ccgacaattc cgtcagcggc cggcgcgcga gctgctcggc
     1441 gccgaccaat tcgagcgccc ggtgcacctg cgcccgggtc tcggcagtga gaatgggcag
     1501 accgggccgg aaaccgtccg caacgcaggc gacgaaatcc cagccgctga tccgaagcgc
     1561 cggcaaagcg gcggcggtct gcggccgata gccgatcgca ggattgccgc gcgtcgccgg
     1621 cttatcgagc acgcggatcg taccattcgt cgggctcagc aggccaagaa tggcccgcat
     1681 caatgtcgtc ttgccggcac cattcggccc tagcacgccg atgaattggc cggcgggaat
     1741 gttcaggctg atatttgcaa ggacattatg gccgccgagc gccagtgtga cgtcacgcaa
     1801 ttcgatggcg ttcatgagcc tctatttcgg cagggcgtga tcgacagcat cgagttcgct
     1861 tgacatccat tgctgatagg caacgccgcg tggctcggtt tcggtcgcgc cgacgacggg
     1921 gatgctggat tgcttcgcca gtttctccat gcgttccgca acggggtcgg acgcctgact
     1981 gttatatact aacatgacca ctttgtggtt ttttagatcg tcctcgaagc cggcaacgtc
     2041 ggaggcgctc ggctccgtgt tgttcatgac cgcgatctgg aacccctgat tgcggctggt
     2101 gaggcccagc gcatcgagca tatagccaaa aacgggttcc gttgccgtaa cagcacggcc
     2161 tgcgagccgg gcttggagcg tcacgatctt ggtgtggatc ggctgcaatg aggcctcgaa
     2221 ttgcgccagg cgcttgtcgt aagccgcctt gtgcgacggg tcgatcgcgc caaggtcggc
     2281 cgcgaacgcc ttggcgaagg cggtcatgat acggatgtcg taccagatgt gcggattgtc
     2341 gctggacttc ttgccgacga gatcggccac gacaatgctc ttgcgatcgg cgctgttgct
     2401 tgcgctcaga agcttctgca cccaggggtc gtaatcggcg ccgctggcga tgacgatctt
     2461 ggcccccgag atcgcccgcg cgacggaggg gctcgcctcg aaaagatgcg gatcttcgtc
     2521 aggattgctg agaatgctcg tcacgctgac gtcgtcgccg ccgatctgct tggccacgtc
     2581 gccatagaaa ttttccgccg cgacgaccgc gacgggcgca gcctgtgctc cgacagggct
     2641 catggcggcg ctggcgacgg cgagagcgaa gaaaagcttt tggcgatgcg cgcgcatcgc
     2701 accgatgggg aacggcatgg aaaacctcac aagactgcca cggacaaaaa ccgctgcgtg
     2761 taatagtata acatatatgg caatcttcgg gcaccgcccc agtcacgatt attttatgaa
     2821 gagcgccaca ccgcgtcggc ccgcgcggtt gcggactttt tatcgcccga gtcttcttat
     2881 cgcccgagtc ttcggctaat cgcggcgcat gtcacgacgt ctttttcttc ccttcgccat
     2941 cctggtcctc ggtttcgccg gtgtcgggct cggatcgttc ctgactcttt ccaaacaggc
     3001 cggtgtgccc attccgtcca tcggtggccc gttccgcctc gcgtcgtcga acggcggcga
     3061 ggcgaccgac gccgatttca aggggcatcc gttcctggtc ttcttcggct atacgcattg
     3121 ccccgacatc tgcccgacgg aattgtttca gatgtcgcag atgttgaagg cagccggggc
     3181 ggacaagccg cttaaggtcc tcttcatcac ggccgatccc gagcgggata cggcgccggt
     3241 gatgaaacaa tatctgtcga gcttcgactc acgcatcatc ggcctgacgg ggacgcgggc
     3301 gcaaatcaat gcggttgaga aggaatatcg cgtttatgcc cgcaaggtgc cgtccgcgaa
     3361 cggcaaggat tattcgatgg accacaccgc tgtcatttac ctgatggaca agcagggcca
     3421 gttcgtctcg gctttcgata tcgagcggcc gccgcaggtc gcggcgcagg atctcgaagc
     3481 ctatctctga gcagcctcag taaaacccga tcgggaaata tttgaggtag atgtcggtat
     3541 agacgccgcg ccgggcgaga tcgaccaagg cataatcaag cgcgcggcgt aaatcgtcgt
     3601 tgcccttctt gaccgcgatg ccgacaccct cgccgaaata attgctgtct gtgtaaggac
     3661 cgccgcgaaa ggcgcagcaa ttgttcgagt ccttaccggc aagccagaag ctcgatgtga
     3721 tggcctcgtc gacaccggcg tcggtctggc cgccgcgcag cgccgcgtag agttcgccgc
     3781 gattggcgaa ggcctcccgc ctgacatgcg gaaagaaggc tttgagataa gcctcatggg
     3841 cggtgccacc gatgattccg acaggcttgc catccaaccc ctcgggcgtt gcttcgaacg
     3901 tcgcacccgt gcgggccacg aagcgcgccg gcgtcgtgta atagggcgcg gtgaattcaa
     3961 accgcgtgtg gcttgcggca tcgatccgca acgaggcgat gagggcgtcg ccgtcccccg
     4021 cgttcagcgc gttttcaaga aggtcgaagc ggcgggcctg gatggtgcag gcaatcttga
     4081 gttcttcgca aatcgcgcgc gccagatcga tatcgaaacc ggtcagcgtc ccgtccgcca
     4141 aagcaaaatg aaatggcgga tacgtgtcgt ccgttagaaa ccgcagagta tgcaggctgc
     4201 cgagatcggg cttttccaga cgattttgca gatcgaaata gcctgggacc gtggcggctt
     4261 gtccaagggc gagagtcggc gccgttaacg caacgagaag aagaattccc gccgcgcgct
     4321 ttgcgcgaaa atatgtcttg ctgagaatgc ccatggttaa gctaatgtaa aaatccgttc
     4381 cagcggttgg cgtcagcgtt ttccagggcg tttgtcttgt ccccaacatg gtcacagcag
     4441 gcgtctcttc gccgcgacat gaggcttggc aaatcggcac gcgccaaccc agtcccggcg
     4501 cgaaaaactt ccgccgccaa accaagcgaa cccctcgccc tcgcgccttc gacattctcg
     4561 cccgcgcgcg gcacgtcgca tctgccaagc gagatcggct ttctcgcgca ctatggcgtc
     4621 tccttcgtgc gtttggcaaa tgccgtggaa acggcccgta ggcagggcgt cacggccgat
     4681 cgcgttctca tcgccgaagg gcaaatatcc gaagactatt tttatcgggc actcgcgcgt
     4741 catttgcggc tgacgttcat cgatgcggat gtcgcgctca aggctgccgc gccctatccg
     4801 caaatgatcg aagccggcgc cgtcgctttg gcgccgggct agggcgcggc cttcctcatc
     4861 gccccgcgcg ggcatggcga tcggccgttt gatcgccgtc ttgagccggc gtgggccggg
     4921 taccaatatc gctcttacga caccgtcaca cttgtcacga ctcgttcgcc aagctgcgcg
     4981 ccgcgagctt tgaccttcgc gacttcgaca ccaatctttg cgccaggggc agtatgaatg
     5041 tgtcgcaggg tatcgcgctc gcggccctcg ccagccttgc ggcatttttt ctgcccctgg
     5101 cgccggccgt ctctgtcatg acgggcgccg ctttcctcgg ccttattctt cttgcgacat
     5161 tttggctgcg tctttcggct tgcgctgcgt ctcctaaaac agcacggccg ccgtcgcgcg
     5221 cgctcagcga tgaggaactg cctttatatt caatcgtgat cgcgctctat cgcgaggcgc
     5281 gcgtcctgcc gcagcttctc ggtgccctcg acgcgctcgc ctatccgcgc gccaagctgg
     5341 acgtgaaact ggtcctcgaa caagacgatc gcgaaacgct cgaagccttg cagagtcttg
     5401 gggtaccgcc gttctgcgaa ttgacgtcgc gccgccggga gcgccgcgga caaagccacg
     5461 cgcgctcaat gtcgcgttgc cgctgctcag aggcgaattg gttgcggtct atgatgccgg
     5521 ggatattccc gatccgcagc agcctcgcct cgcggcttcg cgtttcgccg cggcgccagc
     5581 gcgacttgcc tgccttcagg cgcaactcgc catagacaat atcggtgacg gctggctgcc
     5641 gaactgtctt atggcgcaaa cgcgcgtttg cgccataaga ccgctgccga agcttttcgc
     5701 cattgaatac gccgagctct ttgatgtgat caatacagga ttatcggcgc tgaggattcc
     5761 gtttccgctg ggtggcagat ccaaccattt ccgcacggat gtctttcggc gtgttggcgg
     5821 ctgggatgct tggaatgtca cggaggacgc cgacatcggg tttcgcctcg cgcgctttgg
     5881 ctatgattcc gaaacattgg cctcgacgac ctatgagtaa gcacggatct cgctcgccgc
     5941 gttcttcaag caacggcggc gctggtgcaa gggctggtat cagactttca tcgtcctttg
     6001 gcgccggccg tggcgcctgt tgcagcaggc agggtttgcg cgcagtggcg cgctgacact
     6061 ggctctgctg gcgaatattt tgggttcgtt ggc
//
LOCUS       DASWOG010000265         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_299744,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000265 DASWOG010000000
VERSION     DASWOG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299744"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1008)
                     /gene="ispH"
                     /locus_tag="VGB93_05400"
                     /gene_synonym="lytB"
     CDS             complement(76..1008)
                     /gene="ispH"
                     /locus_tag="VGB93_05400"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0145579.1"
                     /translation="MKVILAQPRGFCAGVVRAIDIVEGALEKYGAPVYVHHEIVHNGH
                     VVDGLRARGAQFVESLAEVPRGAATVFSAHGVSRAVERQAKRRNLAVFDATCPLVAKV
                     HTHATSYVEEGRVLILIGHAGHPEVEATIGRVAAPVHLVQSLEDVAALVLADSTPVAY
                     VTQTTLSVDDTKSIIAALEERFSNIVGPDSKYICYATQNRQAAVRALSRLVGVVLVVG
                     ATNSSNSNRLREIAVRAGVPSYLIADRAELDLGLVREANTIGLTAGASAPEVLVEGVI
                     EALRGLGPLDVVLLPGVQENVEFRLPFEFLATSA"
     gene            1248..>2513
                     /locus_tag="VGB93_05405"
     CDS             1248..>2513
                     /locus_tag="VGB93_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0145580.1"
                     /translation="MDSQLNGAAPAKAPQIGLIGRAIVALVDRCSRSPWTVIIAALLI
                     AGASAWYAGAHFAINTNTNDFLSAKLPWRQRLAELDKAFPQRQDDIIVVIDGRTPELA
                     EGAAQKLFARLTTRPDLYRSVTRPDGGPYFDRVGLMFQPVSLVQRNVGGLLKTEPFLA
                     ALSSDPSLRGVMDAFAFINRGVRVQAGTFDDFSRPIFALSGALENVLAGRQQFFSWQA
                     LFSGQPPEQRQLRHFLSVQPHLDYAALQPGIIPSNFIHQSAKDLGLTPADGVTVRLTG
                     TVPLADEEFSTIKEGSGLNGLLTALIVIGIIWWALRSVRIVFAVAFSVAVGLCVTAAV
                     GLLMVGALNLISIAFAVLFVGIGVDFGIQFSVRYRQERFTHPDFYTALRAAAVRAGSQ
                     VAFAAAATTCGFYSFLPTDYRGVSELGLIA"
BASE COUNT          492 a          814 c          695 g          512 t
ORIGIN      
        1 taaccgccga tttgcagcgc ctgcagtaac ggaataccca tctaatgccg gctcctatat
       61 gagtggctca attgttcaag ctgacgtcgc aaggaattca aaaggcagcc ggaattcgac
      121 attctcctga acgccgggca gaagcacgac gtccagcggc cccaaccctc gcaaagcttc
      181 gattacccct tcgaccagaa cctccggtgc cgaagcgcca gctgtcaggc caattgtgtt
      241 tgcctcacgc acaagaccaa ggtcaagttc cgccctgtcg gcgatcaaat agctcggcac
      301 tccggcgcgc acggcgattt cacgtaagcg attggaatta gagctatttg tggccccgac
      361 aacgagaacc acgccaacca gccggcttaa agcccgcacc gccgcctgac gattttgtgt
      421 cgcgtagcag atatacttcg agtccggtcc gacgatgttc gaaaaccgct cttccaacgc
      481 cgcgatgatc gatttcgtgt catcaacgct gagcgtcgtc tgcgtcacat aggcgaccgg
      541 cgtgctatca gcaagcacaa gcgcggccac atcctccaag ctttgcacca gatggactgg
      601 cgccgctacg cgcccgatcg tcgcctcgac ctcgggatga ccagcatggc cgatgagaat
      661 caggacgcgg ccttcttcaa cataagaggt ggcatgcgta tgaaccttgg ccacgagcgg
      721 acaggtcgca tcgaaaactg caagattgcg gcgcttagcc tgccgttcga ccgcgcggga
      781 gacgccatga gcactgaaaa ccgtcgcagc tccacgcggc acctccgcca ggctttcaac
      841 gaattgcgcg ccccgcgccc tcagcccgtc aaccacatgg ccgttatgga cgatttcatg
      901 gtgaacgtag acgggcgcgc catacttttc gagcgccccc tcgacgatat cgatggcccg
      961 aaccacgccg gcacaaaatc ctcgcggttg ggccaaaatc accttcattc cgagaccaac
     1021 cccaatatta gcccgcgcgg gtcataacgg cggacactag agcttgcttt tttgcaccgc
     1081 gcgcttttaa gaaagtcccg gcgaggtctc cctttgccga actaaactcc aaacgtttta
     1141 gttaaggcag cattgcccaa atacaccatc gtgaaattgg tctgacggcc gaaatcgcgc
     1201 tgcccttgca aagatgccac acatttcgat cgttgatgtt ggagatcatg gatagtcaac
     1261 tgaacggcgc cgcgccggcg aaagcccctc aaattgggct cattggccgc gcaattgtcg
     1321 cgctcgtcga tagatgctca cgctcgccct ggacggtgat tatcgccgcg ctgctcatcg
     1381 cgggcgccag tgcctggtat gcgggcgcgc atttcgccat caataccaac accaatgact
     1441 ttctgtcggc gaaactgccc tggcggcaac ggctcgccga actcgacaaa gccttcccac
     1501 aacgccagga cgacatcatc gtcgtcatcg acggcaggac gccagagctc gccgagggcg
     1561 cagcgcagaa gctttttgcg cggctgacga cccggcccga tctgtaccgg agtgtgaccc
     1621 ggccggacgg cggcccttat ttcgatcgcg tcggtctgat gtttcagccg gtttcgctgg
     1681 ttcagcgtaa cgtcggcggc ctgctgaaga ccgagccctt cctggctgcg ctcagctcag
     1741 atccgtcgct gcgcggcgtg atggatgcgt tcgcctttat caaccgcggc gtgcgggtgc
     1801 aggccgggac gttcgatgat ttctcacgcc ccatattcgc cctgagcggt gcgcttgaaa
     1861 atgtactggc tggccggcaa caattcttct cctggcaggc actcttcagc gggcagccgc
     1921 cagagcaaag gcaattacgc cacttcctct cggtccagcc gcatctcgac tacgcggcgc
     1981 tgcagccggg tatcatcccc tccaatttca tccatcaaag tgcaaaagac ctcggcctga
     2041 cgccagcaga cggcgtcacg gtgcgcctca cgggaacggt gccactcgcg gacgaggaat
     2101 tctcgacgat caaagaaggc tcgggcctga atggcctgct caccgcactc atcgtcatcg
     2161 gtatcatctg gtgggctttg cgctcggtcc gcatcgtgtt cgccgtggcg ttcagcgtcg
     2221 ctgtcgggct ttgcgtgacg gcggccgtcg gccttttgat ggtcggggcg ctcaacctga
     2281 tttcgatcgc cttcgctgtg cttttcgtcg gcatcggcgt cgatttcggc atccagttca
     2341 gtgtccgcta tcgccaggaa cgcttcacgc atcccgattt ctacaccgcg ctacgtgctg
     2401 ccgcggtgcg cgccgggagc caggtggcgt tcgccgccgc ggcgacgacc tgtggtttct
     2461 attcattcct gccgaccgat tatcgcggcg tctccgagct tggcctgatt gcc
//
LOCUS       DASWOG010000266         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_244296,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000266 DASWOG010000000
VERSION     DASWOG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244296"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /gene="nusA"
                     /locus_tag="VGB93_05410"
     CDS             <1..1446
                     /gene="nusA"
                     /locus_tag="VGB93_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0145581.1"
                     /translation="KTGEVRFSRLLQVVEQIENDAKELTLTEAHKKNPAAQVGDWIAE
                     TLPPFDFGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGEIVNGVVKRVEYGNVIID
                     LGRGEATIRRDEMIPREQFRPGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFRQE
                     VPEIYDGVIEVRAVARDPGSRAKIAVISRDSSIDPVGACVGMRGSRVQAVVNELQGEK
                     IDIIPWSGDAATFIVNALQPAEVVKVVLDEDSERIEVVVPDDQLSLAIGRRGQNVRLA
                     SQLTGWDIDILTEAEESERRQKEFVERTNTFMNSLDVDEVVGQLLASEGFRSVEELAF
                     VEPSELASIEGFDADTAEEIQNRARNHLAKIEAELEAKRKELGVADDLHEIDGLTTAM
                     LVKLGENDVKTVEDLAGCATDDLTGWSERNKDNETVKTAGYFDGMDISREDAEAFIMK
                     ARVKAGWIDEAALAAPEAAAEAEDAQAPAAD"
     gene            1479..2144
                     /locus_tag="VGB93_05415"
     CDS             1479..2144
                     /locus_tag="VGB93_05415"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0145582.1"
                     /translation="MDRQTKAELASERTCIVTRTKGAPESLLRFVCAPDGAVVPDLRH
                     KLPGRGVWVTADAATVAKAVKRKAFARGFKAEVRVAESLAADVADLMRRDCLNSLSLA
                     KKAGQVVAGFAKIGAAAQDGSVAALINATDGAQDGTRKLRQVLRRAGADVPEINLFTE
                     AQLSLALGGANVVHAALARGALAANFMSQCRRLELYLSPPLAEAVERAESALSTTVDE
                     SMG"
BASE COUNT          505 a          749 c          837 g          453 t
ORIGIN      
        1 aagaccggtg aggtgcgctt ctcgcgcctg ttgcaggtgg tcgagcagat cgagaacgac
       61 gccaaggaac ttacgcttac cgaggcgcac aagaaaaatc ctgccgcgca ggtcggtgac
      121 tggatcgccg agaccttgcc gccgttcgac ttcggccgca tcgccgcgca atcagctaaa
      181 caggtgatcg tgcagaaggt gcgcgaggcc gagcgcgacc ggcaatatca ggaattcaag
      241 gatcgcatcg gcgagatcgt caacggcgtc gtcaagcgag tcgaatacgg caacgtcatc
      301 atcgatcttg gccgcggcga ggccaccatt cgccgcgacg agatgatccc gcgcgaacaa
      361 ttccgtcccg gcgatcgcgt ccgcgcctat gtctatgacg tgcggcgtga gcagcgcggc
      421 ccgcagattt tcctgtcgcg cacgcatccg caattcatgg ccaagctctt ccgccaggaa
      481 gtgccggaaa tctatgatgg cgttatcgag gtgagggcgg tcgcccgcga ccccggctcg
      541 cgcgccaaga tcgcggtcat ctcgcgcgat tcctcgatcg atccggtcgg cgcctgcgtc
      601 ggcatgaggg gttcgcgcgt ccaggcggtc gtcaacgaat tgcagggcga gaagatcgac
      661 atcatcccct ggtcgggcga cgccgcgacc ttcatcgtca atgcgctaca gccggcggaa
      721 gtcgtcaaag tcgtgctcga cgaagattcc gagcgcatcg aggtcgtcgt gccggacgat
      781 cagctttcgc tcgccatcgg ccggcgcggc cagaacgtcc gtctcgcttc gcaactcacc
      841 ggctgggaca tcgacatcct gacagaggcc gaggaatccg agcgccggca gaaggaattc
      901 gtcgagcgca ccaatacgtt catgaattcg ctcgatgtcg atgaggtcgt cggccaattg
      961 ctggcgtccg agggcttccg ctcggtcgaa gaactcgcct ttgtcgagcc gtcggaactc
     1021 gcctcgatcg aaggtttcga cgccgatacg gcggaggaaa tccagaaccg ggcgcgcaac
     1081 cacttggcga agattgaggc cgagctcgaa gcaaagcgca aggaactcgg cgtcgccgac
     1141 gatttgcacg agatcgacgg tctcaccacc gccatgctgg tcaagctcgg cgagaacgac
     1201 gtcaaaacgg tcgaagatct cgcgggttgc gccaccgacg acctgactgg ctggagcgag
     1261 cgcaacaaag acaacgagac ggtcaagaca gccggctatt tcgacggtat ggacatctct
     1321 cgcgaggacg ccgaagcctt catcatgaaa gcacgcgtca aggcgggctg gatcgacgag
     1381 gcggcgcttg cggcgccgga agcggcggcg gaagctgaag acgcccaggc gcccgcggcg
     1441 gattaggcct ctgcggcgga ttgattggag atgtgcggtt ggaccgccag acgaaagccg
     1501 aacttgcctc cgagcgcacc tgcatcgtca cgaggacgaa aggtgcgccg gagtccctgt
     1561 tgcgcttcgt ctgcgcgccg gatggcgccg tcgtgccgga tttacggcat aaattgcccg
     1621 gacgcggcgt ctgggtaacg gcggacgccg cgacggtcgc caaggcggtc aagcgcaagg
     1681 ctttcgcgcg cggcttcaaa gccgaggtgc gggtcgcgga aagcctggcg gcggatgtgg
     1741 ccgacctgat gcggcgcgac tgcctgaatt cgctctcgct ggccaaaaag gccggacagg
     1801 tcgtcgcggg ctttgccaag atcggggccg cggcacagga cggaagcgtt gcggcgctga
     1861 taaatgccac cgatggcgct caagatggca cccgcaagct gcgccaggtc ttgcgccgtg
     1921 ccggcgcaga cgttccggaa attaatctct ttaccgaagc acaattgagc ttggcactcg
     1981 gcggcgcaaa tgtggtacat gctgcgctcg cgcgcggcgc cctggcggct aatttcatgt
     2041 cccagtgccg gcgtctcgag ctttatctat cgccaccctt ggcggaggcg gtcgagcggg
     2101 ccgagagcgc gctttcgacg acggtcgatg agagcatggg gtaacgacgg cttaaacgtg
     2161 gtagtattct ggccaattct gcaggcgctt cttcgggcag cttcttgtag taaggcttgg
     2221 gaaaaacgac ataggttcta gaaagtcgcc caatagggcg cctcgcggtt caggcgagat
     2281 gtgtcgaggc tccgctgaag gtcgaaggat ccgaggggcc ggggccggcc gttgcgccgg
     2341 cttttggttt tgatcgctgc gcgctttccg gctggataaa tatctggccg atcggaaatc
     2401 gcgccagacc ttgatgttgg ggcctgttcc gaaaaagtcg tacaactttt tcggaacagg
     2461 cctggagctt ttgattgggc ttaagcccgg attgggaacg aatgactgac tcgaaaaatt
     2521 ccggcgacag cacactgacc gtcg
//
LOCUS       DASWOG010000267         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_152572,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000267 DASWOG010000000
VERSION     DASWOG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152572"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..554
                     /locus_tag="VGB93_05420"
                     /pseudo
     CDS             1..554
                     /locus_tag="VGB93_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173493.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            644..1669
                     /locus_tag="VGB93_05425"
     CDS             644..1669
                     /locus_tag="VGB93_05425"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0145583.1"
                     /translation="MFLSLRSLLVSALLPLSMAASGAEKAPDSIDAGLRQQYVRPSAT
                     PYPDDDPYSPAKETLGRALFFDHLLSGSGTHSCATCHSPSLSFGDRLPRAVGDAGRPM
                     AVRSPTLLDVAFIDRYGWTGKFPTLEKVSFAPIMAAGNLNLTEQALIARLSAQAVYVK
                     AFAQVFPNKKIDRENISAALATYVRSIRSTQAPFDRWIMGDETAVSPAAKRGFAIFNG
                     KGNCAQCHSGWTFTDGSFHDIGIGKGKDIGRGALFPTSIKLRYAFKTPTLRNVAERGP
                     YMHDGSVATLEAVIDLYNSGGVDRPSRSELIHPLRLSANEKADLIAFLKTLSGDTEVV
                     SLPASAR"
     gene            complement(1682..1930)
                     /locus_tag="VGB93_05430"
     CDS             complement(1682..1930)
                     /locus_tag="VGB93_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145584.1"
                     /translation="MLEEKRPRKGIGKAMNAIIASLQLPNEEKMISALSVLSLLIGTS
                     LAYAAQRVPSYNVRLEQSGGVLFILGLALVGTVLPRFC"
     gene            2043..>2308
                     /locus_tag="VGB93_05435"
     CDS             2043..>2308
                     /locus_tag="VGB93_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145585.1"
                     /translation="MELRWLGWRHLTAVMGLAVAGSFCWVAEASAHVKWFCFYNVAGQ
                     PRGLENVLCPNFEMLLLTAFTLLLFGYIVEWSVVGTALLAALNRV"
BASE COUNT          464 a          694 c          647 g          503 t
ORIGIN      
        1 gtggcatgat cctgtcacgc ctctaattca tcggcaccca catccataac ttctttcgtc
       61 agaccggccc tgcgctttcg cctttcaatg ccggggtgat gttgaaggcg ctcgaaaccc
      121 ttccgctgcg cgtcaaccat caggccgcga gcgcggcgcg catcgccgat gcacttgccg
      181 agcatccgaa cgtgacacgt ttgatctatc cggggcgccc ggaccatcct caggccgcca
      241 tcatccggcg gcagatgtcg ggcggtggca ccctcgtgac gtttgaagtc ccgggtggca
      301 aggcggccgc cttcgccgta tcgaatgcgc tctcgatcat caaaatctcc aataatctgg
      361 gtgacgcgaa aagcatcatc acccaccctg caacgaccac tcaccagcgg ctgacgccgg
      421 aggcacgggc gaagatgggg atcagcgacg gaatgctgcg gctgtcggtc ggcctcgaag
      481 atcccaacga cctgatcgcg gatttgtcgc atgcgctcgc ggccctcgcc caaccgggct
      541 tggcggcgga ataggccacg tcatgcgcgc cgtggcgaaa gcggcgcaaa aataacgaat
      601 tatcaacggt ttccgcgcca ggctgcaggc gccggagacc gcgatgtttt taagccttcg
      661 cagtctgctg gtgtcggccc ttctgccgct ttcaatggcc gcctcaggcg ccgagaaagc
      721 accagattcg atcgatgccg ggctgcgcca gcaatacgtg cgtccgagtg cgacgcccta
      781 tcccgacgac gatccctatt cgccggcgaa ggagacgctt ggccgcgcgc tgtttttcga
      841 tcacctgctt tcgggttccg gcacccatag ctgcgcaacc tgccacagcc cgtcattgtc
      901 gttcggcgat cgtcttccgc gcgctgtcgg cgatgcagga cgtcctatgg cggttcgctc
      961 gcccacactc ctggacgtgg cctttatcga ccgttacggc tggaccggca aattcccgac
     1021 acttgagaaa gtctcgttcg cgccgattat ggcagcggga aatctcaatc tgaccgaaca
     1081 agccctgatc gcgcggctct cggcccaggc tgtctatgtc aaagcgttcg cgcaggtttt
     1141 tccgaataag aaaatcgacc gcgaaaatat ctcggcagcg cttgcgactt atgtacgttc
     1201 gatccgctca acgcaggcgc cgttcgaccg ctggatcatg ggcgacgaga ccgccgtcag
     1261 ccctgcggcc aaacgcggct ttgcgatctt caatggcaag ggcaattgcg cgcaatgcca
     1321 tagcggctgg acgttcaccg atggatcatt ccacgatatc ggaatcggca aaggaaaaga
     1381 catcggtcgc ggcgcgctat tccccacttc gatcaagctg cgctacgcgt tcaaaacgcc
     1441 gactctgcgc aatgtcgccg agcgcggccc ctatatgcac gacggttctg tcgcgaccct
     1501 cgaagcggta atcgatctct acaatagcgg cggcgtcgat cgtccgagcc gttcggaatt
     1561 gatccacccg cttagattgt cggcaaacga aaaggccgat ctgatcgcgt ttctcaaaac
     1621 gctgagcggt gacaccgagg ttgtcagcct gcccgcatct gcgcgctgaa cgtgccgtgc
     1681 tttagcaaaa gcgcgggagc accgttccaa ctaacgcgag ccccaaaata aaaagcacgc
     1741 cgcccgactg ttcgaggcgc acgttatagc tcggcacccg ttgtgcggca taggccaagc
     1801 tcgtgccaat cagaagactc aaaacgctca aagcggaaat catcttttcc tcgttgggaa
     1861 gttgaagaga cgcgatgatc gcgttcattg ccttaccaat cccttttcgt ggccgttttt
     1921 cttccagcaa cgttaatgcc gtgggtttta tccggcgcga ttaaccactt gttaagaata
     1981 gcgaagacgt agcgttaaaa ttcgattaaa ttacttctat tggaatttgt acggggacta
     2041 aaatggaatt gcgttggttg ggctggcgtc atttaacagc tgtcatgggc ctcgctgtgg
     2101 ccggaagttt ttgctgggtc gcggaagcta gcgctcatgt gaaatggttc tgcttttaca
     2161 atgtcgccgg gcagccccgc ggccttgaga acgttctttg cccaaatttt gaaatgttac
     2221 tgctgacggc gttcactctg cttctgttcg gttatatcgt tgaatggagc gtggttggga
     2281 cggcgttgct tgcggccctc aatcgcgt
//
LOCUS       DASWOG010000268         6907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_24459,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000268 DASWOG010000000
VERSION     DASWOG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24459"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGB93_05440"
     CDS             <1..264
                     /locus_tag="VGB93_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY0145586.1"
                     /translation="LVSLVLAGFVKTPLNDSIDAVKPFEVSDAQAAAIIKNGLARGRA
                     VIAFPMALYFATRLARILPPRFVDRLFTRFSVNVPETQERVKS"
     gene            373..1668
                     /locus_tag="VGB93_05445"
     CDS             373..1668
                     /locus_tag="VGB93_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0145587.1"
                     /translation="MILIEIVRSILEVILGIAIFGLVAVGVSFLILIAINIVELATGK
                     LWREPVPPLKLSDAELPHVLVQIPAFNEGKIAADALFAAATLSWPKDKLHIQLLDDST
                     DETSELAEAATRQLRVQGFDVAHVRRDDRDGFKAGALAHGMTKNDAPIVALLDIDFRA
                     PRDWLLIAVPYLLADPKAGFLQSRCEFSNYRTNWLTRVQGLMLDAHYVMEQATRYRAG
                     WLFQFNGTGGLWRREAIEAAGGWMGDSLSEDLDLVVRAELAGWHGVFLMDPVVPGLVP
                     DRLDHWRVQQRRWSNGFVQVARKLLGEVWGSDWALSRKISASALILIQAFYLCAATAT
                     LSLLACVVLRGFNVVPYLPVIEFLLVLIAIVALGMTLLPYLILRRGSVWQYLTTVVSV
                     PPMMIYMSVSNAPSILKTVFRGSRESFMRTPKSHQNAGT"
     gene            complement(1754..2779)
                     /locus_tag="VGB93_05450"
     CDS             complement(1754..2779)
                     /locus_tag="VGB93_05450"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0145588.1"
                     /translation="MNMAVRDTGAESKEPPHDFLVAEPMRPHPQRRRAILAAHPEVAK
                     LFVTDPLTAFITLGVVIGQTAIAGYLGHLGLGYWWLTLIVAYFVGAFANHAMFVVIHD
                     AVHNTIFKTPDGNKWVAILADLPNAFPTAMGFRCYHQKHHSHLGDYEYDADLPGHLEA
                     KLAGNRWYGKAFWLFGFAFWQLSRLGRLKGTVPIWGKWTYINTVAVIAYDVAVLYFFG
                     WNALFYLFASFWFSVGLHPLGARWIQEHFTFDPQQETYDYYGPLNIVALNIGYHNEHH
                     DFPEIPWRLLPELKRMAPEFYDNLKAHKSLTKLFLTFIFDPRYTLYSRVDRSILRRAN
                     EAAAATR"
     gene            complement(2924..4093)
                     /locus_tag="VGB93_05455"
     CDS             complement(2924..4093)
                     /locus_tag="VGB93_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145589.1"
                     /translation="MSEIKIVPVKGLGGFLEFCKVQRLIYKGMKGFAAQLDAERWALY
                     GSKLNPHFKSVDWQAWLAYKDGKIAGRIQAQIYKELVPLGASPAQFGCLDTIEDDAVL
                     GALLEAAETWLRDRGAGIVHGPFSPYIWGEVGMLVEGYTAVPMIFMPWNPSYLPAMLE
                     RHGYAKARDLISYRYAITEKDKDRGAGIVSRPEWRDRLKIRTIDLNNIKAEAVQIVDI
                     FNDAWSENWGFVPFTYEEFMASADSLKYIMPPECGFMVELDGVAQAFAVVLPNLNEIT
                     EDLEGKLLPFGLPKVVSRVRNHQYKTARLMLFGVRKALQRKAIGGAVILAFIQEIRRR
                     SAQYPSIEHCEFGWVLEDNMGMRKPIEMSGAEIDKVHRIYGKDLTAAAAPKAVAA"
     gene            complement(4201..5430)
                     /locus_tag="VGB93_05460"
     CDS             complement(4201..5430)
                     /locus_tag="VGB93_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0145590.1"
                     /translation="MAHRIKSLADYANRRFGLAGKAYLEQYNPFFVETDTARHDAQAH
                     GRNFVSFANYDYLGVSGHPAVKAAALNALDEQGVGALASRLVGGQRSTHHVLEDKLAS
                     FLGVESVLTLVSGYLTNVTAISHMMNTHDAIFLDELSHNSIISGAKAASATSHFFQHN
                     DLDHLESLLEQKRGSYRHVLIAVESLYSMDGDIVDLPRLIEIKDRYNCWLLVDEAHSI
                     GVLGQDGRGLCEHTGVDPNQIDLIVGTLSKTFASCGGFIAGKKPVTEWLRYTLPGFVF
                     SVGISPVISAAASTALDIVQKETWRIEKLRRNAELFLELAHEAGLDTGPAIGRGVVPI
                     LFSDGLETVAASRHLMANGYYVPPIFQIGVPKNQPRLRFFLSATHREEDIRGVINLLA
                     RPQNQALVKGEPVSATP"
     gene            5683..6456
                     /locus_tag="VGB93_05465"
     CDS             5683..6456
                     /locus_tag="VGB93_05465"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0145591.1"
                     /translation="MASDTILVETHEKVGLIRLNRPQALNAINAELMVALNEALDALE
                     ADAGISAIVLTGSAKAFAAGADIKEMQHKTYAEALIPDFIANWQRLSRVRKPTIAAVA
                     GFALGGGCEIALMCDFIIAADNAKFGQPEIKLGIMPGGGGSQRLTRAIGKARAMELCL
                     TGRIIDAAEAERAGLVARIVPADQLEAEALKTAAAIAEHSLPALLLTKEAINRVAESS
                     LAEGLLFERRAFQSLFATHDQKEGMAAFVEKRPAKFENR"
     gene            6641..>6907
                     /gene="rpsT"
                     /locus_tag="VGB93_05470"
     CDS             6641..>6907
                     /gene="rpsT"
                     /locus_tag="VGB93_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0145592.1"
                     /translation="MANTSSAKKAVRKIEHRTTVNRSRRSRMRTFVHKVEEAIAAGNH
                     EAASEALKAAEPVIVRAAQNGIIHRNTASRKVSRLTHSIRALAKA"
BASE COUNT         1408 a         1995 c         2101 g         1403 t
ORIGIN      
        1 ttggttagcc ttgtcctcgc gggcttcgtc aaaacgccgc tgaatgattc gatcgacgcc
       61 gtcaaaccat tcgaggtcag tgacgctcag gcggcggcca ttatcaaaaa cggcctggcg
      121 cgggggcgcg cggtgatcgc ctttccaatg gcgctctatt tcgcgacgcg gctggcgcgt
      181 attctgccgc cgcgtttcgt cgatcggctg ttcactcgct tttcggtcaa tgttcccgaa
      241 acgcaagaaa gagtgaaatc ataaacttag cgcctgccgg gttttccttg ttttgattat
      301 tgttttcgtg cttcaaacgc gaacgaggct ggccagagag gccgcgcaga tcaacccgga
      361 aaggcgcgca gcttgatcct catcgaaatt gtgcgctcga ttctcgaagt cattctcgga
      421 attgccattt tcggcctcgt tgccgtcggc gtcagctttc tcatcctcat cgccatcaac
      481 atcgtcgaac tcgcaacggg caagctttgg cgcgagcctg tgccgccact caagttgagc
      541 gacgccgaac tgccgcatgt gctcgtccag attcccgctt tcaacgaagg caagatcgca
      601 gcggatgcgc tcttcgccgc cgcgacgctc tcctggccaa aggacaagct gcacatccag
      661 cttctcgacg acagcaccga cgagacatca gaacttgcgg aagcagccac acggcaatta
      721 cgggtgcagg gcttcgatgt cgcacacgtg cgccgcgatg accgcgacgg ctttaaggcg
      781 ggcgcgctgg cgcatgggat gacgaagaac gacgcgccaa ttgtcgcctt gctcgacatc
      841 gactttcgcg cgccgcgcga ctggctgctc atcgcggtgc cttatcttct cgccgatccg
      901 aaggctggtt tcctgcaatc gcgctgcgaa ttttccaatt accggacgaa ttggctgacg
      961 cgcgtgcaag gcctgatgct cgacgcgcat tacgtgatgg agcaggcgac acgctatcgc
     1021 gccggctggc tgttccagtt caacggcacg ggcggcctct ggcggcgcga ggctatcgag
     1081 gctgccgggg gttggatggg cgattccctc agcgaggatc tcgatctggt ggtgcgcgct
     1141 gagcttgccg gctggcacgg ggtctttctg atggaccctg tagtgccggg cctcgtaccg
     1201 gatcgactcg accattggcg ggtgcagcag cggcgctggt cgaacgggtt tgtgcaggtt
     1261 gcgcgtaagc tgcttggcga agtatggggc tcggattggg cactgagccg gaagatctcg
     1321 gcgagcgcac tgatcctcat tcaggcattt tatctgtgcg cggcgaccgc gactttgtcg
     1381 cttctcgcct gcgtcgtact gcgcggcttt aatgtcgttc cttatttgcc ggttatcgag
     1441 ttcctgctgg tcctgatcgc gattgtggcc ctaggcatga cgctgctgcc ttatcttatt
     1501 ttgcggcgcg gctcagtctg gcaatatctc accaccgtcg tctcggtgcc gccgatgatg
     1561 atctatatga gcgtttcgaa cgcgccgtcg atcctgaaga cggttttccg cggcagccgc
     1621 gagagtttta tgcggacgcc gaaatctcat cagaacgccg gcacgtagga attctagcct
     1681 ggccgcgatt acggccaggc attcattatc ttggtaaggc cgaagccgtt tcgttgcatt
     1741 tgcgacgcaa attctagcgc gtcgcggcgg cggcctcgtt ggcgcggcgc aggatcgaac
     1801 gatcgacccg cgaatagagc gtgtaacgcg gatcgaagat gaaggtgagg aacagcttcg
     1861 tcagcgattt gtgcgccttc agattgtcgt agaactccgg cgccatgcgc ttcaattcgg
     1921 gaagcagccg ccacggaatt tcgggaaagt cgtggtgctc gttgtggtag ccgatgttga
     1981 gcgccacgat gttgagcggc ccgtaataat cgtaggtttc ctgctgcgga tcgaaggtga
     2041 aatgctcctg gatccagcgc gcgccgagcg ggtggagacc aacggaaaac cagaatgacg
     2101 caaagagata gaagagcgcg ttccagccga agaaatagag caccgcgaca tcataggcga
     2161 tgacggccac cgtattgata taggtccact taccccagat cggcaccgtg cccttgagcc
     2221 ggccgaggcg ggagagctgc cagaaggcaa atccgaagag ccagaacgcc ttgccatacc
     2281 agcgattgcc ggcaagcttc gcttcgagat ggccgggcag atcggcgtca tactcatagt
     2341 cgccaagatg cgaatgatgt ttctggtgat agcagcgaaa gcccatcgcg gtcgggaagg
     2401 cattcggcag gtcggccagg atcgccaccc atttgttgcc atccggcgtt ttgaaaatcg
     2461 tgttgtgaac cgcgtcgtgg atgacgacga acatcgcgtg gttggcgaag gcgccgacaa
     2521 aataggcaac gatcaaggtc agccaccaat agcccaggcc gagatggccg agataaccgg
     2581 cgatcgcggt ctggccgata acgacgccga gcgtgatgaa cgcggtgagc ggatcggtga
     2641 cgaaaagctt ggcgacctcg ggatgcgcgg ccagaatggc tcggcgccgc tgcggatgcg
     2701 gccgcatcgg ctccgcgacg aggaaatcat gagggggctc tttgctctca gcccctgtat
     2761 ctcgaaccgc catattcatg tgcgaagcct cgcttatcgc gtcccgagtt ctcgcccccc
     2821 agcgaattca tatctaccag taaccaaaaa cccgcgcctt gcgaaggtct acggtcaact
     2881 ttggaagcga accaatcgtc gaacgaaggg tccgatcctc gtcttaggcc gctaccgcct
     2941 ttggcgccgc agccgctgtc aggtccttgc cgtagatgcg gtggactttg tcgatctccg
     3001 cgcccgacat ttcaatcggc ttgcgcatac ccatattgtc ctcgagcacc cagccgaatt
     3061 cgcaatgctc gatcgacgga tattgcgcgg atcggcggcg gatttcctgg atgaaagcaa
     3121 ggatgaccgc gccgccgatg gccttgcgct gcaaggcttt gcgcacgcca aagagcatca
     3181 gacgtgcggt cttgtattga tggttgcgaa cgcgcgagac caccttcggc aaaccgaacg
     3241 gaagcagctt gccttcaagg tcttcggtga tctcgttcaa attcggcagg acgaccgcga
     3301 aggcctgcgc aacgccgtcg agctcgacca tgaacccgca ttcgggcggc atgatatatt
     3361 tcagcgagtc ggccgaggcc atgaattctt cgtacgtaaa cggcacgaag ccccagttct
     3421 cgctccaggc gtcgttgaaa atatcgacga tctgtaccgc ttcagccttg atgttgttga
     3481 ggtcgatcgt gcggatcttc agccggtcgc gccattcagg ccgggagacg atcccagccc
     3541 cccggtcctt atctttctcg gtgatcgcat agcgatagga gatcaagtcg cgggctttcg
     3601 cgtagccgtg gcgttcgagc atcgccggca aataggacgg gttccagggc atgaaaatca
     3661 tcggcacggc cgtgtagcct tcgacaagca tacccacttc gccccagatg tacggcgaga
     3721 aaggtccgtg cactattccg gcgccgcggt cgcgcagcca ggtctccgcc gcttcgagca
     3781 gggcgccgag tacggcgtcg tcttcgatcg tatcgagaca gccgaattgc gccggcgaag
     3841 cgccgagcgg cacgagctct ttgtagatct gcgcctgaat gcggccagcg atcttgccat
     3901 ccttataggc aagccacgcc tgccaatcga ccgacttgaa atgcgggttg agcttcgagc
     3961 cgtagagggc ccaacgctcg gcgtcgagct gcgcggcaaa acccttcatg cccttgtaga
     4021 tgagccgttg gactttgcag aattcgagga aaccgccaag ccctttgacg ggaacgatct
     4081 tgatttcact catccgattt ccgttgccga cgctcatcga gacttgaagc ggcgctgata
     4141 cgaattctca tttcgaagcg ttcatgcgac cgaaaagtct cactttttcg gcgttggtct
     4201 tcagggcgtg gcgctaacag gctcgccctt gaccagcgcc tggttttgcg gccgggcaag
     4261 gagattgatg acaccgcgga tatcttcttc gcgatgcgta gcggagagga aaaagcgcag
     4321 ccgcggctga ttttttggca cgccgatctg gaagatcggc ggcacataat agccattcgc
     4381 cataagatgc cgcgaagcgg cgacggtctc aagaccgtca ctaaagagga tgggcacgac
     4441 gccgcggcca attgccgggc cagtatcgag cccggcctca tgcgcgagtt cgaggaacag
     4501 ctccgcgttg cggcgcaatt tttcgatacg ccaggtttct ttctggacga tatccagtgc
     4561 cgtcgaagcc gctgccgaga tcaccggcga gataccgacg ctgaaaacaa agccgggcag
     4621 ggtatagcgt agccattcgg tgacaggctt cttgccggcg atgaagccgc cgcaagaggc
     4681 gaaggtcttc gagagcgtac cgacgatgag atcgatctgg tttggatcga caccggtatg
     4741 ttcgcaaagg ccgcgaccgt cctggccgag cacgccgatg gaatgtgcct cgtcgaccag
     4801 gagccagcaa ttatagcggt ccttgatttc gatgagacgc ggcaaatcga cgatgtcgcc
     4861 gtccatgctg taaagcgatt cgactgcgat cagcacatgg cggtaggagc cgcgcttctg
     4921 ctcaagcagc gattccagat gatcgagatc gttgtgctga aagaaatggc tcgtcgcgct
     4981 tgcggccttg gcgccgctga tgatgctgtt gtgggacaat tcgtcgagaa aaattgcatc
     5041 atgcgtgttc atcatatgcg aaatcgctgt cacattggtc agataacctg aaaccagcgt
     5101 gagaaccgat tcgacgccga ggaaactggc gagcttgtct tccaaaacat gatgggtcga
     5161 gcgttgaccg ccgacgaggc gcgaggcgag ggcgccgacg ccctgttcgt caagcgcgtt
     5221 gagcgccgcg gccttcacgg ccggatggcc ggagacgccg aggtaatcgt aattggcgaa
     5281 gctgacaaaa ttccgcccgt gcgcctgggc gtcgtgacga gccgtatcgg tctcaacaaa
     5341 aaacggattg tattgttcga gataggcttt gccggcgagc ccgaagcgtc tgttcgcata
     5401 gtctgcgaga ctttttatgc gatgtgccaa gtcagattgt ccatatgttc gagcctagcc
     5461 ttgcgagaat cgacaaggcg gaccgggcct tctatgtccc tggagccata acattcccga
     5521 cggcagccgg caaaagacgc gaacaatcgg ccgtgggcgg cgtcgggcta tagagcccgc
     5581 aattagctca acgtctttag aagcacaagt ttaggatgat aagtcaaata gggttacaaa
     5641 gcgtaacaac cgcagcgcac aaagcagaaa agggcctcgc gcgtggcaag tgacacgatt
     5701 ctcgtcgaaa cccatgagaa ggttgggctg attcggctca accgcccgca ggcgctgaac
     5761 gccatcaacg ccgaactgat ggtagcgctc aatgaggcgc tcgatgcctt ggaggctgac
     5821 gccggaattt ccgcgatcgt gctgacgggc tccgccaagg ccttcgcggc tggcgccgac
     5881 atcaaggaaa tgcagcacaa gacctatgcc gaggcgctga tcccggattt catcgccaac
     5941 tggcagaggt tgtcgcgcgt gcgcaagccg acaattgcgg cggtcgccgg ctttgcgctc
     6001 ggcggcggct gcgaaatcgc cctgatgtgc gacttcatca tcgccgccga caacgccaaa
     6061 ttcggccagc cggaaatcaa actcggcatc atgcccggtg gcggtgggtc gcagcgcctg
     6121 acacgggcga tcggcaaggc cagggcgatg gagctttgcc tgactggccg gataatcgat
     6181 gctgcggagg ccgagcgggc ggggctcgtc gcccggatcg tgcctgccga tcaattggag
     6241 gccgaagctt tgaagactgc ggccgccatc gcggaacatt cgttgcccgc actgctgctg
     6301 acgaaagagg cgatcaatcg cgtcgccgag tcgagcctcg cggaagggct tctcttcgag
     6361 cggcgcgcct tccaatcgct tttcgccacg catgatcaga aagaggggat ggcggctttt
     6421 gtcgagaagc ggccggccaa attcgagaac cgctgattta tctggtgctt ccaggccggt
     6481 cttgccgcag caaagcgtat acggcgcggt gtgaaaacac tcgtggggcg ttgacggccc
     6541 gcgcgagcgc aattataacc tgccgcttcg agtttgcgca tccgccgatg cgaccttttc
     6601 cggttgactt cggccgagcc tttccaggac atttctatct atggcgaata cctcgtcggc
     6661 caaaaaggcc gtccgtaaaa ttgagcatcg cacaacggtc aaccgttcgc gccgttcgcg
     6721 catgcgcacc ttcgtccaca aggttgagga agcgattgcc gccggcaatc atgaagccgc
     6781 ctccgaggcg ctgaaggctg ccgagccggt gatcgtccgc gctgcccaga atggcatcat
     6841 ccaccggaac acggcgtccc gcaaagtctc acggttgacg catagcatcc gggcgctggc
     6901 aaaggca
//
LOCUS       DASWOG010000269         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_263004,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000269 DASWOG010000000
VERSION     DASWOG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263004"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..978)
                     /locus_tag="VGB93_05475"
                     /pseudo
     CDS             complement(1..978)
                     /locus_tag="VGB93_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(1084..>1516)
                     /locus_tag="VGB93_05480"
     CDS             complement(1084..>1516)
                     /locus_tag="VGB93_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY0145593.1"
                     /translation="KFSKLVRKFEQRCEDLEDVSATERHRTRIHAKNLRYGVEFLDSL
                     VRITGRKEHRKNQRAFVSALKRIQNFLGEENDVLMAHSYFAGLGQDEDTVAADEAAQV
                     AGAAVAAHARASSKRKFHKTVAKARRDLFEVKPFWLDLARH"
BASE COUNT          322 a          464 c          423 g          307 t
ORIGIN      
        1 gcggtcgagc accagcacgg cgtcctgcgt cgtaacaacg atgacattat cgacgccgac
       61 caccgtcgtg agatgatcgt cggaacggat atgcacgttc ttggcgtgca tcacgatgcc
      121 atggccacgc accgaattgc cgtcgctgtc atgcggcgat aattcatcga ccgccgccca
      181 ggtgccgaca tccgaccagc cgatatccgc cggcaccacg gcggccttgt cggtatgttc
      241 catcactgca taatcgatcg aaattttcgg cgcccgcgag aaggcgtcct tatcgagaag
      301 caggaattcg agatccttgc gcgctttgcc aagagcttcc gccgccgcat caaggacgag
      361 cggcgcgtgc tggcgtaatt ccgcctgcat gacgtcggcg cgaaagatga aattgccggc
      421 gttccagagg tagccttcgg cgcaataccg ggccgcggtt tcggcgtcgg gcttttcgac
      481 gaaggcttcc gcctgcaaaa ccgagccgtc atccatgacg ggtttgccgg tcttgatgta
      541 gccgtagccg gttgcgggcc ccgcgggctt gacgccgagc gtcacgatat aaccggctgc
      601 ggcggcaccg gcagcagcac ggcacgacgc gatgaacgcc gcgtggtcct tgatgacatg
      661 atccgcagca aagatcgcaa cgatcgtttg cggcgagatt tccaccgcga gttccgtgcc
      721 gaccgcgaca gccggcgccg aatcccgccg gattggctcc agcaccacgc gcgcggcgcg
      781 gccggtttac tttacctgct cagagacgag gaaacggtaa tcctggttcg aaatcacaat
      841 tggcggctcg aacaacggat cgtcgacgtt ctccaacgcc agttgaaagg tcgaacgcgc
      901 gccaaacagc gaaatgaatt gcttcggcag actttcgcgc gattccggcc aaacccgggt
      961 gccggaaccg ccgcacatga tgatcgggag aattttcaaa atcgtcgttc cgaaagttga
     1021 aatcccaact taggctttgc cgccgctagc gggcaagcca aaagccaaat tagcatgaat
     1081 aagctaatgc cttgccagat caagccaaaa tggcttcacc tcaaaaaggt cccggcgcgc
     1141 ttttgcgacg gttttatgaa atttccgttt gctgctcgcg cgcgcatggg cggcgacagc
     1201 agcacccgcg acctgagcgg cttcgtccgc cgccaccgta tcttcgtcct ggcccagacc
     1261 tgcaaaatag gaatgtgcca tcagcacatc gttttcctcg cccagaaaat tctgtatgcg
     1321 ttttagcgca ctgacaaagg cgcgctgatt cttgcgatgc tctttgcgcc cggtgatccg
     1381 cacaagacta tctagaaatt ccacgccata gcgaagattt ttcgcatgga tccgcgtgcg
     1441 atgacgctct gtggcgctca cgtcctccaa atcctcgcag cgctgttcaa attttcggac
     1501 gagcttactg aatttg
//
LOCUS       DASWOG010000270         7356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_354225,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000270 DASWOG010000000
VERSION     DASWOG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354225"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGB93_05485"
     CDS             complement(<1..353)
                     /locus_tag="VGB93_05485"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0145594.1"
                     /translation="MGRADALRPDRHKHDYRPLARSELPIDTEALARFLIGKTVVREL
                     SEGITSGRIVETEAYVIGDAAGHGFRGMTQRNHSLFLERGHAYVYLAYGISNMLNVSS
                     EASGVGAGVLIRALEP"
     gene            complement(332..712)
                     /locus_tag="VGB93_05490"
     CDS             complement(332..712)
                     /locus_tag="VGB93_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0145595.1"
                     /translation="MDDREIRAALDRHWAASDANDFDEEHQIYREDAVLEYPQSGERI
                     RGRRNIQSSRAAQPNLKRFTIRRVLGAGDLWITEYVLTYDARPSYTVSIMEFVDGKVA
                     RETQYFGDPFEPGPSRAQWVERMP"
     gene            complement(801..1772)
                     /locus_tag="VGB93_05495"
     CDS             complement(801..1772)
                     /locus_tag="VGB93_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY0145596.1"
                     /translation="MLTAYREKGDDWRVEGADLPDNIIWLDLLDPTPEEQKLVETRAK
                     IRVPSKEALSEIEASSRLIIQHGVLYLSTPMVAKGEAESTEISPAGFVLTRNLLVTVR
                     FKQLAPFDNVAERIRTDETLDCSVGVFTAILEAIVDRGADVLEHLAATTDKVSKSIFH
                     AGSSDPKHPTRSTARLHQILTDIGINSNRLAQARDSLLGVGRLAAFVSEIAHEWVPEP
                     FSVRLHAISKDVASLTDYETHLSDKVQFLLDATLGYISIEQNDLFKVLTIVSVVGVPP
                     TLLAGIWGMNFKNMPELNWAWGYPLAWLAIILSAILPLMWFKRRGWF"
     gene            complement(1848..3215)
                     /locus_tag="VGB93_05500"
     CDS             complement(1848..3215)
                     /locus_tag="VGB93_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0145597.1"
                     /translation="MGEHFIPVEAPFHSRPLNRLEIALVARRNALEIIPALAYRQPIV
                     SGDMLVRWHMLADPVGLKRVLLDNQQNYPKSEIMRRMLRPAIGESLFNADGAEWKWQR
                     QAVAPIFTHRNVVALAPAMSATAARACERLAACGDRAELVGEMLTATFDVICDVALSG
                     RKHFDGRAFGDAVTRYFKMAGRPSLLDFFGFPRWFPRPGEWRVGSSVRTMHRMVAQAI
                     EARRGRSTGLDTGLGDDLLDRMLAAEDPESGRKMTPEDLVHNMQFFIVAGHETTALAI
                     SWALYLLANMPEHQDRARAEATAQLSGRVAEAGDLDAMPFIRQVLDEAMRLYPPVGLV
                     ARTVLESDELCGRVMRPNDTIFLPIWALHRHEMLWDTPNAFDPDRFSPSNSAGRDRYQ
                     YLPFGAGPRVCVGANFAMMQAQIILATLVQRFRFTPSLPAPRPIMTMTVRPEPGVFLS
                     LEPLH"
     gene            complement(3238..4041)
                     /locus_tag="VGB93_05505"
     CDS             complement(3238..4041)
                     /locus_tag="VGB93_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145598.1"
                     /translation="MRLRPFLLNIGCIFSLFIVGTLAGPSRSFASDESWGVPAWLRAH
                     IGEGEDDIAPIVLQRARALYLQKVHEGAVRNSCYFAMDATRPNDMGGSGHRFYVICES
                     SQSFRAISAGQEAVFTRAFLQFDGEGETANARERRVGGHAAVALKGLCLMKRPSSPYA
                     DQDGYVPRGTLVEYSGGRSDGCTSWTPSDARQIMSMVRDDPTTLYIYPDAADIRAAAR
                     GGSYWNASCLREIGSPRFWSRETLEPLLAEYKQAHPAPPPRPIPICKGP"
     gene            complement(4179..5618)
                     /locus_tag="VGB93_05510"
                     /pseudo
     CDS             complement(4179..5618)
                     /locus_tag="VGB93_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
     gene            5765..>7356
                     /locus_tag="VGB93_05515"
     CDS             5765..>7356
                     /locus_tag="VGB93_05515"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY0145599.1"
                     /translation="MNFQPRGVSDPAIQGSLSYQSEIEAAEALLKTKETWNGVSAEAV
                     ARMRLQNRFKTGLDIARYTAALMRADMAAYDADSTKYTQSLGVWHGFIAQQKMISIKK
                     HFGSTDRRYMYLSGWMVAALRSEFGPLPDQSMHEKTSVPALIAELYTFLRQADARELN
                     DIFRSLDAARKEGDTAKEKALIEKIDNFQTHVVPVIADIDAGFGNAEATYLLAKKMIE
                     AGACALQIENQVSDEKQCGHQDGKVTVPHEVFLAKIRACRHAFLELGVEDGIIVTRTD
                     SLGAGLTQQIAVSHTPGDLGDQYNAFLDCEEVTAADARNGDVIINRNGKLLRPKRLAS
                     NLYQFRAGTGVDRCVLDCITSLQNGADLLWIETEKPHIEQIAEMVDRIREVVPNAKLT
                     YNNSPSFNWTLNFRWQVYDAMKKAGKDVSQYNRAELMKEEYDTTPLAIEADERIRTFQ
                     ADSAKRAGIFHHLITLPTYHTAALSTDNLAKEYFGEQAMLGYVKNVQRAEIRQGIACV
                     RHQNMAGSDIGDDHKEYFAGEAA"
BASE COUNT         1530 a         2222 c         2192 g         1412 t
ORIGIN      
        1 ggctcgagcg cgcggatcag aaccccggcg ccgacccctg atgcctcgct cgaaacattc
       61 agcatgtttg agatgccgta ggcgagataa acataggcgt gcccgcgctc aagaaacagc
      121 gagtgattgc gctgcgtcat cccgcggaag ccgtgccctg cggcgtcgcc gatgacatag
      181 gcttcggtct cgacaatacg accgctggtg atgccttcgg ataactcgcg caccactgtc
      241 ttgccaatga gaaatcgggc gagggcctcc gtgtcgatcg gcagctcgga gcgcgcgagc
      301 ggccgatagt catgcttgtg gcggtcaggc ctcaaggcat ccgctcgacc cattgcgcac
      361 gcgaaggccc cggctcgaac ggatcgccga aatactgcgt ctcgcgggcc acctttccat
      421 cgacgaactc catgatgctc acggtgtaag acggccgcgc gtcataggtt aggacatatt
      481 ccgtgatcca gagatcgccc gcaccgagaa cccgccgtat cgtaaagcgt ttcaggttcg
      541 gctgcgccgc gcgagacgac tgaatgttcc gccggccgcg gatgcgctcg ccggattgcg
      601 gatattcaag caccgcgtcc tctcgataga tctggtgctc ttcgtcgaag tcgttggcgt
      661 cagacgcggc ccaatggcga tccagagccg cccgaatctc ccggtcgtcc atagaaatct
      721 cctgaccttc accgtcgatg ggcaagccat cgtagcatgc gaagacctgg aacgggtctt
      781 ctagatgaac ggcggcgtgc ctagaaccag ccgcgcctct tgaaccacat gagtggcaat
      841 atcgcgctca gaatgatggc gagccacgcc aaaggatatc cccacgccca gttcagctcc
      901 ggcatgttct tgaagttcat gccccagata ccggcgagca acgttggcgg cacgccaacc
      961 actgagacga tagtcagcac tttaaacagg tcattttgct cgatcgaaat atagcctagc
     1021 gtcgcatcta gcaggaattg caccttatcg gacagatgcg tctcgtaatc ggtcaaactc
     1081 gccacgtcct tggagatggc atgcaaccgg acgctgaatg gttctggtac ccattcgtgg
     1141 gcgatctcac tgacgaaggc ggcgagacgt cctacaccca aaagagaatc gcgtgcctgg
     1201 gccagacgat tagagttgat accgatgtcg gtaagtatct ggtgtaaacg cgccgttgat
     1261 cgtgtgggat gtttagggtc agatgagccg gcatgaaaaa ttgattttga gaccttgtcg
     1321 gtcgtcgcgg caaggtgttc gaggacgtca gcaccgcgat cgacgatcgc ctccagaatg
     1381 gccgtgaaaa ctccgacgct gcaatcaagc gtttcgtcgg ttcgtatcct ttccgccacg
     1441 ttatcgaagg gtgcaagctg tttgaatctc acggtcacca gaagattgcg cgtcagcacg
     1501 aatccggccg gtgagatttc tgtactctcc gcttcgcctt tcgcgaccat tggcgtgctt
     1561 aagtagagaa cgccatgttg gatgatcagc cggctggagg cttcaatctc gctcagtgcc
     1621 tctttcgagg gtacccgtat tttcgcacga gtctcaacga gcttctgttc ttccggcgtg
     1681 ggatcaagca gatcgagcca gatgatgttg tctggaagat ctgcaccctc gacgcgccaa
     1741 tcgtcgcctt tctcgcggta cgccgtcagc aaaatacaat actcctgtca acgcgtatga
     1801 tgatcgggct cagtgaaccg gggcgcatgc ccaagtcttt aggtccttca atgcaggggt
     1861 tcaagcgaca ggaagacacc aggttctggc cgaactgtca tcgtcatgat cggacgcggg
     1921 gccggcaggc tcggcgtaaa ccggaagcgt tgcaccagcg tagcgagaat gatttgtgcc
     1981 tgcatcatcg cgaaattcgc accaacgcag acccgcggac ccgccccaaa cgggagatat
     2041 tgatacctgt ctcgtcccgc actgtttgac ggcgaaaatc tatcgggatc gaaagcattg
     2101 ggtgtgtccc acaacatctc gtggcggtgg agagcccaga tcggcagaaa gatggtgtcg
     2161 ttcggccgca tgacccgccc gcataattcg tcgctttcca acacagttcg cgcgacgagg
     2221 ccgacgggcg ggtaaagccg catcgcttca tccaggacct gcctgatgaa tggcatcgca
     2281 tccaaatcac ctgcttccgc gacacgcccg ctcaactggg ctgtggcctc tgcccgcgct
     2341 cgatcctgat gttcgggcat gttcgcgagc agatacaggg cccatgaaat cgccaatgcg
     2401 gtcgtctcgt ggccggcaac gatgaagaac tgcatattgt ggacgagatc ttcgggcgtc
     2461 atctttcgcc cgctctctgg gtcctcggca gccagcatgc gatcgaggag gtcgtcgcca
     2521 agacctgtgt caagacctgt gctgcggccc cgccgtgcct cgatcgcctg cgcgaccatg
     2581 cggtgcatgg tgcgcacgga agatccgaca cgccattcgc cggggcgggg aaaccagcgg
     2641 ggaaagccga aaaaatccaa tagcgagggg cggcccgcca tcttgaagta acgcgtgacg
     2701 gcgtccccaa aagcgcggcc gtcaaaatgt ttgcgccccg acaaagcaac atcgcatatg
     2761 acgtcaaacg tcgcggtcag catttcgccg accagttccg cgcggtcgcc gcaggccgcg
     2821 agccgttcgc aggcgcgggc cgctgtggcg ctcatcgcag gcgccaacgc aaccacattg
     2881 cgatgggtga agatcggcgc cacggcctgg cgctgccatt tccattccgc cccgtccgcg
     2941 ttgaacaggc tttccccgat ggcggggcgc aacatgcgcc gcatgatctc cgatttcgga
     3001 taattctgct gattgtcgag cagaacccgt ttgagtccca cgggatcggc gagcatgtgc
     3061 cagcgaacga gcatgtcccc cgaaacgatc ggctggcgat aggccagggc cggaatgatc
     3121 tccaacgcat tccgccgcgc tacgagcgcg atttcgagac ggttcagggg ccggctgtga
     3181 aagggggctt cgaccgggat aaaatgctcg cccactacga tcccctagag gccaacttca
     3241 cggccccttg caaatcggta tcgggcgtgg cggcggcgcc ggatgagcct gtttgtattc
     3301 ggcgagcaac ggctcgagag tttctctcga ccaaaatctc ggagatccga tttctctcag
     3361 gcaggacgca ttccaatacg atccgccccg cgcggcggcg cggatatcgg ccgcgtcagg
     3421 atagatatag agcgtcgttg gatcgtcgcg caccatcgac ataatttgcc gtgcatccga
     3481 tggcgtccag ctcgtgcagc cgtcgctgcg gccgccggaa tattccacca gcgttccgcg
     3541 cgggacgtaa ccatcctggt ccgcgtaggg gctgctaggt cttttcataa gacacaagcc
     3601 cttcaaggcc acagcggcat ggccgccgac ccggcgttcc ctggcgttcg cagtctcgcc
     3661 ttcgccatcg aactggagaa acgcgcgtgt gaaaaccgcc tcctgtcccg ccgaaatcgc
     3721 tcgaaacgac tggctcgatt cgcagatgac gtagaaccgg tgacccgaac cgcccatatc
     3781 gttggggcgg gttgcgtcca tggcaaaata gcaggaattt ctaaccgcgc cttcgtgcac
     3841 tttttgcagg taaagtgcgc gcgccctctg caagacaatc ggcgcaatat cgtcttcgcc
     3901 ttcgccgata tgcgccctca gccaagccgg aacgccccac gattcgtccg aggcgaaaga
     3961 ccgggacggt cccgctaacg tgccgactat gaacaggctg aaaatgcagc cgatattcag
     4021 aaggaaaggt ctcaaccgca tcggatcaat tctcttcgaa gcatcgtctc gttcgggatt
     4081 gatcatatag ggcctcgcgc cgatcggcga tgaacttctt cgtcagtgtt cctcgacgat
     4141 aagcctgcac cgaaggcttg ttgtctggct aagacccctc aatccgcgga gaatccaaac
     4201 gggacgcttg aggtcctgaa gtcgtcggga aggatctcgc gcccgatcgg aggcgccgag
     4261 cgtgctgcac atttgggacg atgacagaca cggcatgcca cgccgatcgg tgtgggctcg
     4321 tcggcacgca gtccctgacc accagcggtg tagatcagct gtcctgcatg ctcagccgcg
     4381 caaccgattg caatggcccg ctcaacgcga gttgcgccga acgagccgcc gcccgcggtc
     4441 acggtgcgcg cgatcgaaaa gaatcgctgg ccatccggca attccagcca ttgcgtaacg
     4501 atttggcgcg gcgtcttgaa gattccataa acctaccata gcgggcaggc gccgccgtgt
     4561 ttggcaaatg ggaagccggc gccgtcgagc agcttggtaa tattgccggc cggatcgaca
     4621 cggatcagga agaacgggac cttctcgaga cccggcctct gcaaggtcgt gacgcgatgg
     4681 gcggtttgct cgaagctggt gccgaagtga cgcgcaagcg cttcgagatc gtaacggcgc
     4741 gtgtcgacag cctttgcaaa ggcggaatac ggcatgatga tcgcggcggc ggcgtaagcg
     4801 gcgagggcgc ggcgcgccag taactcggcg ctcttgctcg cgaacttgcc ggcttccagc
     4861 gccgcatcga tctcatcttc catttcaaga taggcgagct gcttggccag ctcgaagttg
     4921 aggcttgcag tatcgagcga gtcctcgatc aggagctgct tgtgatgcag gtccagacgc
     4981 ctcaccgatc cgagcatgac gctgggcggc agataacgga cctgaaggtt gtggcgaagg
     5041 cgaagccgtt cgatgaatcc ggccgccccg gaaggcgcca agacaaggcg ctccgccgca
     5101 tcgtcgagat tggcaaagtt gttgcggcgt gcggcaagaa accgtctgac gtcggcgacg
     5161 ggatcgtttg catcaggctt gccctggccc caacgcgccg cgccgccctt catcgtcggc
     5221 gctctttgct ccgccaacgc aagctgttct tcgcgatagg ccgtgtagag ccgaaggaag
     5281 gcttcggtca ttccgggata gctgacggca aggtcgctaa tttcgagcgg agggatatcg
     5341 atgtcggaaa acatcggatc cttcaggatg gactgcatcc gcgccgtgtg atcggcgccg
     5401 ccgtcgccgg cgaggtcggc aaggtcgatc ttgtaggtgc gggccagacg aagcagcata
     5461 tccgcggtca ccggccgttg attgcgctcc agaagcgcaa catagggggc ggaaatctcg
     5521 agatcggccg ccatatctgc ctgggtgagc cccagctcgc gccgaagccg tcgcagtcgc
     5581 ggtcccatga aaatcgagcg ggcgccggag gttgccatgt ccagtccttg cgttatgtaa
     5641 tgttcttaca gatatacaat attttattgt aaagcgtgac aagttttctt tcaaacggct
     5701 tgtgagtagc gatgtttcgc gtacaaacgc agcaggtttt ttcgttatta agggatcgat
     5761 cgacatgaac ttccaaccgc gcggggtcag cgacccggcc attcagggct cgttatccta
     5821 tcagagcgag attgaggcgg ctgaagcact cctcaagacc aaggagacct ggaacggcgt
     5881 gagcgccgaa gccgtcgcgc gcatgcgctt gcagaaccgc ttcaagaccg gcctggatat
     5941 cgcccgatat accgcggcgc tgatgcgggc tgacatggcc gcctatgacg cggacagcac
     6001 gaagtacacc cagtcgctcg gcgtctggca cggcttcatc gcccagcaga agatgatttc
     6061 gatcaagaag catttcggct cgaccgatcg ccgctatatg tacctctccg gctggatggt
     6121 cgccgcgctg cgctccgagt tcggaccgct gcccgatcag tcgatgcacg agaagacctc
     6181 ggttccggcc ctgatcgccg aactctacac cttcctgcgt caggcggatg cccgcgaatt
     6241 gaatgatatt ttccgcagcc tcgacgctgc ccgcaaggaa ggcgacacgg cgaaggaaaa
     6301 ggcgctgatc gagaagatcg acaatttcca gacgcacgtc gtgcccgtca tcgccgatat
     6361 cgatgcgggc ttcggcaatg ccgaggcgac atatcttctc gccaagaaga tgattgaggc
     6421 gggcgcgtgc gccctgcaga tcgaaaacca ggtctccgac gaaaaacagt gcggccacca
     6481 ggatggcaag gtgactgtgc cgcatgaggt tttcctggcg aagatccgtg cctgccgtca
     6541 cgccttcctc gaactcggcg tcgaagacgg catcatcgtg acccgcaccg actcgctcgg
     6601 cgccggcctc acgcagcaga ttgccgtgag ccacacgcct ggcgacctcg gtgatcagta
     6661 caacgccttc ctggattgcg aggaagtgac ggccgccgat gcccgcaacg gcgatgtcat
     6721 catcaaccgc aacggcaagc tgttgcgtcc gaagcggctg gccagcaatc tctaccagtt
     6781 ccgcgccggc acaggtgtgg atcggtgtgt gctcgactgc atcacctcgc tgcagaacgg
     6841 cgccgacctg ctgtggatcg aaaccgagaa gccgcatatc gagcagatcg ccgagatggt
     6901 cgatcgcatc cgcgaagtgg ttccgaacgc gaagctgacc tacaacaact cgccgtcttt
     6961 caactggacg ctgaacttcc gttggcaggt ctacgatgcg atgaagaaag ccggcaagga
     7021 tgtgagccag tacaaccgcg ccgagctgat gaaggaggaa tacgacacca cgccgctggc
     7081 gatcgaggcc gacgagcgta tccgtacctt ccaggccgat tcagccaagc gcgccggcat
     7141 cttccatcat ctgatcacgc tgccgaccta tcacacggcg gctctgtcga ccgacaatct
     7201 cgccaaggaa tatttcggcg aacaggccat gctcggctat gtgaagaacg ttcagcgtgc
     7261 cgagatccgt cagggtatcg cctgcgtgcg gcatcagaac atggccggct ccgacatcgg
     7321 cgacgaccac aaggaatatt tcgctggcga ggcggc
//
LOCUS       DASWOG010000271         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_335924,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000271 DASWOG010000000
VERSION     DASWOG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335924"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..>2420)
                     /locus_tag="VGB93_05520"
     CDS             complement(13..>2420)
                     /locus_tag="VGB93_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0145600.1"
                     /translation="ITRSLAQGLVEKGRIILGGDMSFDLVQREVTADELRVLASHGRV
                     TSVALVRAMARRAGGDSALVEIKAVDQSYPTGGTLSLHPAQSLADAFELRNGAYGVAA
                     DATLLARLDLKLGDRFSIGAATFALRAILTNEPDNLAAGIGFGPRVLMSQPALRATQL
                     LRPGALVRWLYRLSLRSGEAPNDVKADVKKALHTAGFEIRTWKNVAPEFSRDLDRFSL
                     FLILVGLSALLIGGIGIANAVASFVDRKTPTIAKLKALGASGIFVFCMMLIEVMAMAG
                     LGMVLGIVAGAALPFVLAAALGALLPFPFAPALFPSEIGAGFLYGILTVLTFAMLPLG
                     RVHDLPVSALFRDEIAPQRPRLKRFYLLLFGFAAGGLALSIVIFSPDRKLALIFIIAA
                     LGAFALLSGAAALVMFGARNIRARDVELRLALANLHRPGALTPIIVLSLGLGLTLVVA
                     LALINGNLQTLFNHGGTGATPDFFFLGVEKTQTPDFRRFVEAHAPQGKLELVPMLRGR
                     IVRLNGVASENAKVSSDSAWALQGDRGITFSAALPEGSKLVAGAWWPKDYAGPAEVSM
                     AADIAAGLGLKIGDAISVNVLGREIAARITNLRKVDWTNLGINFVLVFPPNAFAGAPY
                     SNLATLTFAAPPSDAQITRLVRDVAKAYPDIVSLRVKDALDAVRPVADELAAAVRGAA
                     AIALLAATLVLAGALAAGQQARLYDVVVLKVLGATRWRLLVALICEFGIIGAATALFG
                     LIAGSAAAFAIVRFVMKLDFAWLWPRAFEAAAIALIAAIAFGVLGTWRILGRKPAQFL
                     RNL"
BASE COUNT          561 a          757 c          736 g          366 t
ORIGIN      
        1 ccatgccgaa acctataaat ttcgtaagaa ttgggcgggt ttacgcccca aaatgcgcca
       61 tgtgcccaga accccgaaag caatcgcggc aataagcgcg atggcagcgg cctcgaaggc
      121 acgcggccaa agccaggcga aatccagctt catgacaaaa cgcacgatgg caaacgcggc
      181 ggcgctgcca gcgatgaggc cgaaaagcgc cgtcgccgcg ccgatgattc caaattcgca
      241 gatcagtgcg acgagaagcc gccaccgcgt cgcgccgagg actttcaaca ccacgacatc
      301 atagagccgc gcctgctgcc ccgccgccag cgccccggcg agaaccagcg tcgcggccag
      361 caaggcgatc gccgccgcgc cacgcacggc tgcggccaat tcgtcggcga ctggccgcac
      421 ggcgtcgagc gcgtctttga cgcgcaggct gacaatatcc ggataggctt tcgcgacatc
      481 acgcacgagg cgcgtgatct gtgcgtctga tggcggcgcg gcgaaggtca gcgtcgcgag
      541 atttgaataa ggcgcaccag cgaacgcgtt gggtggaaag accagcacga aattgatgcc
      601 aaggttcgtc caatcgactt ttcgcaaatt cgtaatccgc gccgcaatct cccggccgag
      661 aacattgacg ctgatcgcgt cgccaatctt gaggccgagc cccgcggcga tatccgccgc
      721 catagaaacc tctgccggcc cggcataatc tttcggccac caggcacctg cgacgagttt
      781 ggacccctcg ggcaacgcgg ccgaaaacgt aatgccgcga tcgccctgta gcgcccaggc
      841 gctgtcgctg ctaactttcg cattttcgga cgccacgccg ttgagcctga cgatccgacc
      901 gcgcagcatc ggcacgagtt cgagtttccc ctgcggtgca tgagcttcga cgaagcgacg
      961 aaagtctggc gtttgggttt tttcgacacc gagaaagaag aaatccggcg tcgcgcccgt
     1021 cccgccgtgg ttgaagaggg tctgtagatt gccattgatg agggcgagcg ccacgaccaa
     1081 tgtgagccct aatccaagcg agagaacgat gatcggtgtc agcgcgccgg ggcgatgcag
     1141 attggccagc gccagccgca gttcgacgtc gcgcgcacgg atatttcggg cgccaaacat
     1201 gacaagcgcc gccgcgccgc tcagcaaagc gaaagcaccg agcgccgcga tgatgaagat
     1261 cagcgccaat ttgcggtcgg gcgaaaaaat cacgatggat aatgcgaggc cgccggcagc
     1321 aaagccgaaa aggagaagat aaaatcgctt gagccgcggc cgttgcggcg cgatttcatc
     1381 gcgaaacagc gccgagacgg ggagatcgtg cacccggcca agcggcaaca ttgcgaaagt
     1441 taaaaccgtc aaaatgccat agagaaaacc agccccgatc tccgatggaa acagagccgg
     1501 cgcgaaggga aatggcagca aagccccaag ggccgccgcc aacacgaacg gcagtgccgc
     1561 gccggcaacg atgccgagca ccattccgag gccggccatc gccatcacct cgattagcat
     1621 catgcagaag acaaagatgc ccgaggcgcc caatgctttg agtttcgcaa tggtcggcgt
     1681 tttgcggtca acgaaactcg cgactgcgtt ggcgataccg atgccgccaa tcagcagcgc
     1741 gctgaggccg acgaggatca ggaataggct gaagcggtcg aggtcgcgcg aaaattccgg
     1801 cgcgacgttc ttccaagttc gaatttcgaa gccggcggtg tgaagcgcct tcttgacatc
     1861 agccttcaca tcgttcggcg cttcaccgga tcgcaacgac aggcgataaa gccagcgcac
     1921 caaggcaccg ggccgcaaca attgcgtcgc ccgcaatgcc ggctgcgaca tcagcacacg
     1981 cggaccaaag ccgatgccgg cggcgaggtt atcgggctcg ttcgtcagaa tggcacgtag
     2041 agcgaacgtc gcggcgccga tcgaaaagcg gtcgccaagt ttgagatcaa gacgcgcgag
     2101 caaggtcgca tcggcagcga cgccgtaggc cccatttcgc aactcgaagg catcggcaag
     2161 actttgcgcc ggatgcaggc tcaaagtgcc gccggtcgga taggactgat cgacagcctt
     2221 gatctcgacg agcgcactat cgccaccggc acgccgggcc atcgcccgca ccagcgcgac
     2281 ggatgtgaca cgcccatgcg aggccaggac gcgaagttcg tcggccgtca cctcccgctg
     2341 aacgagatcg aaggacatat cgccgccgag gataatgcgg cctttttcga ccagaccttg
     2401 tgccagcgaa cgggtaatcg
//
LOCUS       DASWOG010000272         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_98100,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000272 DASWOG010000000
VERSION     DASWOG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98100"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGB93_05525"
     CDS             complement(<1..371)
                     /locus_tag="VGB93_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145601.1"
                     /translation="MFFLIRCVFWLWVVFSTIFAQPSGEHGTPHASPSAPQAVALQPI
                     ASNAQAWLERAATRVADRCGSAPATCLAVAAQLSKLAGSGIPASAWPSAQVTQIAPEV
                     PRIPDVPLPPRRPQNFARTARP"
     gene            751..2562
                     /locus_tag="VGB93_05530"
     CDS             751..2562
                     /locus_tag="VGB93_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0145602.1"
                     /translation="MSFALNIEKQITSLVHPNVLPRPAERLRHETFILRRLASSMAVI
                     CLAPLFLAIYGAPALWHALVFIWCIVPIGAVLLLSRSGNLLLAQTICIASFIGAAASV
                     AAAGGPWEAALVWLVLAPFEGALSQNSRLVLGAGALSALAALALILVRSSLASGHAPA
                     LVLPAIAYATFVAYGLVAFQHRRETSAHARSDYYRAVYEMVGDLIVHHASDGTAEFVS
                     PACYELFGLPSADLLGRGLFEHIHVADRPAFLKAISDAAISSRVATATLRLRAPGPHG
                     EVSADTDFRWIELRAQHCSSEAGDATAHSGRVVSTLHDITQIKQREQDYEAARATAED
                     ANLWKDQFLANVSHELRTPLNAIIGFSEILSDEGLSPQEPGKQREYAAIIHKSGQHLL
                     AVVNTILDMSKIQSGAFEIRAEPFPLPPLIDLCCDMVKLKATEGSVELVRAYAEKIDE
                     IIGAKRAYRQVLLNLLSNAIKFTPPRGRVTIKLRPDGNHIIISVADTGIGIAQNDLAS
                     LGSPFFQAGASYDRPYEGTGLGLSVVRGLVGLHGGTISIESEPDRGTCVSVRLPLDSR
                     RLPASKTGTAKIETLPRRSRGDDQRVFSNEMMVKKIA"
BASE COUNT          460 a          791 c          774 g          543 t
ORIGIN      
        1 ggccgggccg ttctggcaaa gttttgcggc cggcgcggtg gcagaggcac atccgggatc
       61 cgtggaactt ccggcgcgat ttgcgtgacc tgcgcgctcg gccatgcgga agccggaatg
      121 ccgctgccgg ccaatttgga gagctgcgcg gcgacggcaa ggcaagtagc cggcgcactc
      181 ccgcagcgat cggcaaccct ggttgcggcc ctctcgagcc aggcttgtgc gttcgaggca
      241 atcggctgca gagccacggc ttgcggcgcg gacggcgacg catgcggcgt gccgtgctcg
      301 cccgaaggct gcgcaaagat ggtgctgaac accacccaaa gccagaaaac gcagcggatc
      361 aaaaagaaca tattcgtctc gtctcaggca gccgcagaac gaccgatgcg ataccctagc
      421 cggagcgcat aaaaatcgtg cgattatcgg cacgacaatt cgagccgcaa gatttacctt
      481 ttgatgacca ttcgccgagc gcgcgggaag ctatctggca agaccttcta aaccatagcg
      541 ggagaattcg cgtctcccgc cgctgcgctt cctctcgggc gtttcatcgc tgccagcgtt
      601 ttgatggtgt ggcttagtgt tcacttaaga cgcgcctgcg aacttgtttt cgtcgaaagg
      661 cggagattcg cagacgagtt gcgatcccct tcgctttgcg gccgtgcccg cagcttgctg
      721 cgccgtcgcg cggaagccgg agttcagcgg ttgagttttg cgttaaatat cgagaagcag
      781 atcacctccc tggtgcatcc aaacgttctg ccgcgtccgg ccgagcgcct gcgccatgag
      841 accttcattc ttcgccgctt ggcgagttcg atggcggtca tatgcctcgc gccgctgttt
      901 ctcgcgatct atggcgcgcc agcgctgtgg catgcccttg tcttcatctg gtgtatcgtc
      961 ccgattggtg ctgttctgct gctgtcgcgc agcggcaatc tgttgctggc ccagacgatt
     1021 tgcatcgcca gtttcatcgg cgcagcggcg tcggttgcag cggctggtgg tccatgggaa
     1081 gccgctcttg tctggcttgt cctcgcgcca tttgaaggtg cgctttcaca gaattcccgg
     1141 ctcgtgctcg gcgccggagc tctgtccgcg cttgcggcgc tcgcgctgat ccttgtgcgt
     1201 tcgagccttg ccagcggtca cgcgccggct ctcgtactgc cggcgatcgc ctacgcgacg
     1261 ttcgtcgcct atggtctcgt cgctttccag caccggcgcg agacgtcggc tcacgcacgt
     1321 tcggactatt atcgcgcggt ctacgagatg gtcggcgact tgatcgtgca tcatgccagc
     1381 gacggcaccg cggaatttgt cagtccggct tgctatgaac ttttcggcct gcccagcgcc
     1441 gatctcctgg gccgtggcct ctttgaacat atccacgttg ctgataggcc ggcgtttctc
     1501 aaagcgattt ccgatgccgc gatatcgtcg cgcgttgcga cggcgaccct gcgtttgcgt
     1561 gcacccggtc cgcatggcga ggtcagcgcc gacacagact ttcgctggat cgaattgcgc
     1621 gcacagcatt gctcatccga ggccggggat gcaacggcgc actcggggcg cgtcgtatcg
     1681 acgttgcacg acatcacgca aatcaagcag cgcgagcagg actatgaggc cgcacgcgcg
     1741 accgccgaag atgcaaatct ttggaaagat cagttccttg ccaatgtcag ccatgaattg
     1801 cgcacgccac tcaacgcgat catcggcttt tcggagattc tgtccgacga gggattgtcc
     1861 ccgcaggagc ccggcaaaca gcgcgaatat gctgcgatca tccacaaatc cggccagcat
     1921 ctgctcgccg tcgtaaatac cattctcgat atgtcaaaga ttcagtctgg cgcgttcgag
     1981 atcagggctg agcctttccc gttgccgccg ttgatcgatc tctgctgcga tatggtgaaa
     2041 ctcaaagcga ccgagggtag cgtcgagctt gttcgcgcct atgccgagaa gatcgatgag
     2101 atcatcggcg ccaagcgagc gtatcggcag gttcttctca atctgctttc caacgcgatc
     2161 aagttcacgc cgccgcgcgg ccgtgtaacg atcaagctgc ggccggatgg caatcatatc
     2221 atcatctcgg tcgcggatac cggcatcggt atcgcgcaga acgacctcgc cagtctcggc
     2281 agcccattct ttcaggccgg cgcatcgtac gatcggcctt acgagggcac ggggctcggg
     2341 ctctctgtcg tgcgcggcct cgtcggtctc catggcggga cgatttcgat tgagagcgaa
     2401 cctgatcgtg gaacctgcgt cagcgtccga ctgccgctcg acagccgccg tctgccagcg
     2461 agcaagaccg gcacagccaa aatcgaaacc ttgccgcgcc gctcgcgcgg tgacgatcaa
     2521 cgtgttttca gcaacgagat gatggtgaag aaaattgcgt gaagctct
//
LOCUS       DASWOG010000273         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_318063,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000273 DASWOG010000000
VERSION     DASWOG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318063"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..606)
                     /locus_tag="VGB93_05535"
     CDS             complement(214..606)
                     /locus_tag="VGB93_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0145603.1"
                     /translation="MVKSELSTSLSHADDLSGVIAAAKGEDGARTVTRVNLAEAVYQQ
                     VGLSRKESAALVEQVLDEITDRLIVGEGVRLSSFGSFSVRHKGQRVGRNPKTGVEVPI
                     KERRVLIFKSSNLLKAQINGVHAEPDSE"
     gene            complement(910..1908)
                     /locus_tag="VGB93_05540"
     CDS             complement(910..1908)
                     /locus_tag="VGB93_05540"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0145604.1"
                     /translation="MSLTRPQNAVLRSVVAGLGSYLPKRILSNADLEKMLDTSDEWIT
                     QRTGIKMRHIAAEDERTSTLATHAARAALADAGLTGEDIDLVIVATATPDYTFPAVAT
                     QVQAAIGMTGGVAFDLQAVCSGFIFALTTADKFLTTGSHKRALVIGAETFSRLLDWQD
                     RGTCVLFGDGAAAMVLEARPGEGTSADRGVLTAHLRSDGRHLSKLYTDGGPSSTGTAG
                     HVRMEGREVFKLAVTMITDVVDAAFAATGTNGEQLDWFVPHQANARIIDASAEKLKIA
                     PQKVVKTVAFHGNTSAASVPLALAAARDDGRIKPGDLVMLEAMGGGFTWGAVLLRW"
     gene            complement(2002..2473)
                     /locus_tag="VGB93_05545"
                     /pseudo
     CDS             complement(2002..2473)
                     /locus_tag="VGB93_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
BASE COUNT          512 a          770 c          705 g          486 t
ORIGIN      
        1 attcgcataa ccgcaagaca ttctgcggca aatcgtggtc gctctgtacc tcgttaatgg
       61 gcctaaatgc gtctgctgtc ttttccattg ttccgcggct ccgattccgg caacttcccg
      121 ctctgatcta acgcaaggaa caatgccagc gcgtttcggc gcaaacgccg aatatcgcgg
      181 cgccgccaat cacatggcgc tcggcgaatc agactactcg gaatcgggct cggcgtgaac
      241 gccgttaatc tgcgctttca gcagatttga cgatttgaag atgagaacgc gccgctcctt
      301 gatcggcact tcgacgccgg tcttgggatt acggccgacg cgctggccct tgtggcgcac
      361 gctgaacgat ccgaacgacg aaagccgaac cccttcgccg acaatcaatc gatcggtgat
      421 ttcgtcgagc acttgctcga caagcgccgc ggattctttg cgcgacaaac cgacttgctg
      481 atagacggct tcggcgagat tgacgcgcgt gacagtgcga gcgccgtcct caccttttgc
      541 agcggcgatt acgccggaca aatcgtccgc gtgtgaaagc gatgtcgaca attcactttt
      601 gaccatcttg ggactcgatg atttctgaat gcgatgactt ggcagcgaag gccgtaattt
      661 tggtctgcgg acaatcgccc gagctcaatg aaattgaaag gggcgccaaa aaaacgtgcg
      721 cctcggcaaa caaaattctc ctgaacattt gatcagcccc gcagttccct tccccgcacc
      781 gcaaatggat taagcccttt gcttgtcttg aactttggcg caaaacatac gtcagcacaa
      841 tagacagaaa gatttgtcct gcaaactaca atgcggacaa aaggcgccgt tgttgcaaag
      901 cggcagcatc taccagcgaa gaagcactgc gccccaggta aaaccgccgc ccatcgcttc
      961 gagcatcaca agatcacccg gcttgatgcg gccgtcgtca cgcgccgccg ccagcgccag
     1021 cggcaccgac gccgccgacg tattgccgtg aaaggcgacg gtcttcacga ctttttgcgg
     1081 agcaattttg agcttctccg ccgacgcgtc gataatgcgg gcgttggcct gatgtggcac
     1141 gaaccagtca agctgctcgc cgttcgtccc ggtcgcagca aaggcggcat ccaccacatc
     1201 agtaatcatc gttacggcta gcttgaaaac ttcccgccct tccatgcgca catggccggc
     1261 tgttccggtc gatgacggac cgccgtcggt ataaagtttc gagagatgcc ggccatcaga
     1321 ccgcagatgc gcggtcaaga cgccgcgatc ggcgctcgtt ccttcgccgg gccgcgcttc
     1381 aagtaccatc gctgccgccc cgtcgccgaa cagcacgcag gtgccgcggt cctgccaatc
     1441 gagtagccgc gaaaacgtct cggcgccgat gacgagcgct cttttgtgcg atcccgtggt
     1501 tagaaattta tccgcggtgg tcagcgcgaa aatgaaccct gaacacaccg cctggagatc
     1561 gaacgcaacg ccgccggtca tgccgatcgc tgcctgaacc tgcgtcgcaa cggcgggaaa
     1621 agtatagtcc ggcgttgcgg tcgcgacgat gacgagatcg atatcctcac ccgtgagccc
     1681 cgcatcggcg agcgcggcgc gcgccgcatg cgtggcaagc gtcgaggtgc gctcgtcctc
     1741 cgccgcgata tggcgcattt tgatgccggt gcgctgggtg atccactcat cgctggtgtc
     1801 gagcattttc tcgagatcag cgttgctcaa aatccgcttt ggaagatatg accccaggcc
     1861 agcgacgacg gagcggagaa cggcgttttg cggtcgggtc aaagatattt gtcctgtttc
     1921 aaacaacgtg acggtcgcct tagagcattt tccgccgcga cagaagtccg aactacctgc
     1981 caaaagtggg gatcaaacgg ctcaggcctc ggcctccgga tcgatggcca gcactgccgc
     2041 gggcacgtcg cgctcggaga gggcatcctg gatcttgcgc agaaactggt cgcggaccag
     2101 tccgtggccg atttccagcg cccgcgcggt gccgaccgcg tcggagccgc cgtggctctt
     2161 gatcaccacg ccttcgaggc caagcaggat accgccgttg acgcggcgcg ggtccatctt
     2221 gttcgacagc gaacggaacg cctgccgcgc cagcaattag ccgagtttcg aggcgaaact
     2281 gccgcgcagc gcctcgcgca gataggcgct gatctgcttg gccgtcccct cggcagtctt
     2341 gagcgcgata ttgccggcaa agccttcggt cacgaccaca tcgacgctgc ctttgccaat
     2401 gtcgtcacct tcgacaaagc cgcgatagtc gaaatcgggc gaattgagtt cgcgcaaaat
     2461 ccgcgaggcg gcc
//
LOCUS       DASWOG010000274         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_171127,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000274 DASWOG010000000
VERSION     DASWOG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171127"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB93_05550"
     CDS             <1..207
                     /locus_tag="VGB93_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY0145605.1"
                     /translation="ASLMTKHKLQAALYAPLRVVLYENSNGGTTFEYDKPSTLFGQFG
                     DAQVDKVAHDLNQELEQALRRAAE"
     gene            268..720
                     /locus_tag="VGB93_05555"
     CDS             268..720
                     /locus_tag="VGB93_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017965601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0145606.1"
                     /translation="MKVLSINGQSHQVDVPDDMPLLWVLRDVLGMTGTKFGCGIAQCG
                     ACTVHLAGQPVRSCLLPVSAIGARPVATIEAVGQTPNGHKIQGAWLDKEVVQCGYCQS
                     GQIMSATALLNQTPHPTDADIDAAMSGNICRCGTYVRIREAIHAAASV"
     gene            731..2887
                     /locus_tag="VGB93_05560"
     CDS             731..2887
                     /locus_tag="VGB93_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017965600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0145607.1"
                     /translation="MGYDLPSRRFVLKAGAAVGGGLLLGLHLPSLARASADMSSDFEP
                     NAFLRIDPHNTITLILPHTEVGQGIYTSAAMLMGEELEIGLDQVNIEAAPPDIKKYID
                     PLLGDQATGGSASTRADWVRLREAAAVARVMLISAAAKKWNVDPASCRAERGIIHHDA
                     SGKSASYGDVASDAAQLPVPSNVKLKDPSQFKLIGTSAKRIDTPSKVNGTALYGIDII
                     VPDMRFGTVAITPVKGGKLASMDEAAARRVPGVHDVIRNDEGDAIAVIGDHMWAAKQG
                     LEALNLKWDAGENGDVTLETIISTMDRASHNEGVVAKKEGDAAAAIKGAATQLSAIYQ
                     SPFLSHSPMEPLNCTLHIRPDSAEIWLGTQVPVRAQKAVASVAGLPQDKVTVYNQLMG
                     GAFGRRLDIDSVEVAASIAKHVSYPVKLVWTREEDLRHDYYRPYYYDRVAAGLDANGK
                     LVGRTHRVTGPSILARWAPPAFKNGLDDDAVLCAAETPYEIPNEYVDYVRDEPRAMNT
                     SWWRGVGPTHNLFVVESFIDELAHAAKQDPVEFRRGMLKKNPRALAVLNLAAEKSGWG
                     SPLSRGTGRGVELQFAFGSYLCCVLEVEVTPPGEISVRRAVVAIDCGMTVNPDTVQAQ
                     IQGGLILGLGTAMYNEITLTGGAIDQSNFHDYRAMRINEAPKIEVYQIRNGEKPGGIG
                     ETGTAAAAPALGNAIFAATGKRLRRLPFGRGQLSGT"
     gene            2930..4222
                     /locus_tag="VGB93_05565"
     CDS             2930..4222
                     /locus_tag="VGB93_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0145608.1"
                     /translation="MKKWLAFAFGLAALAGVAAAVFFFLPAGLEPVAASAAQPSGDAL
                     IARGEYLTKAADCEACHTNPGGASFAGGRRFDLPFGTLYAPNLTPDRAHGIGAWSNAE
                     FVRALHHGIGRNGEDLYPAFPYASYALLSDDDALAIRAYLATVKPVAATPPANDLKFP
                     FNQRYLMRAWKLLFVPGHRFEADTSKDATWNRGAYLIGPLGHCGECHTPRDLMQGLDD
                     SRKFAGTKQVGWLAYNLTSDPDHGLGGWSDAQLAQYLSVGHAEGHGPASGPMAEVIGF
                     SLRYLRPDDIRAMVTYLRSIPPQPDGPPAAGRGAPAGVQKPPLGARIFVEACAGCHLP
                     SGHGRQSPWAALAGSHTTADPNANNLLQVLAQGTQIKTSQGLMFMHAFTSAYTDEEVA
                     ALANYTCAQFGQTTCHVTADQVHKVRGDVPAKTEEASQ"
     gene            complement(4223..5365)
                     /locus_tag="VGB93_05570"
     CDS             complement(4223..5365)
                     /locus_tag="VGB93_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0145609.1"
                     /translation="MRRWLSAALVLVCMNLMMGCAARPDSGFLATTAYEAPSATEHTI
                     LIASTRERDARPGTLYSGFRADWLDYASATMSVPPTHVDGKIEWPQTPPGDPRRDFAV
                     RDAGYIDGDRAFLATVNAELAKRPPGHRKIFLFVHGFNTMFAEALYGFTQVVHDAHTD
                     HVPVLFTWASRGKLTDYLYDNNSATAARDQLAHTIRLLTDSNAEEVNILAHSMGNWVF
                     VEAMRQIKMQGGLKHPEKIGLVLLAAPDIDIDVFKSDLRAYGKPKKPYYIVLSKDDKA
                     LALSKFLAGGKARVGDDGNVQDLAKLGAVVIDLTNVHGDDPSDHNKYAQIAAIAPQLE
                     HVLQSGIQKNHVGMSDQTDQMASALGAVVSVPVNIMGNSFSIVSSR"
BASE COUNT         1123 a         1691 c         1658 g         1029 t
ORIGIN      
        1 gcatcgctga tgaccaagca caaattgcaa gccgcgctct acgcgccgct tcgtgtcgtg
       61 ctctacgaga attcaaatgg cggaacgaca tttgaatacg acaaaccttc aacacttttc
      121 ggacaattcg gtgatgcgca ggtcgataag gtcgcgcacg acctcaatca ggagctggag
      181 caggcgttgc ggcgggccgc cgagtgaagc gccgcgcctg taatgaatca tattggacat
      241 gaagcgctgg gcacgaggac aggaagcatg aaagttcttt cgatcaacgg acaatcgcac
      301 caggtcgacg tgccggatga catgccattg ctctgggtct tgcgcgacgt cctaggcatg
      361 acaggcacga agttcggctg cggcatcgcc caatgcggcg cttgcaccgt ccatctggcc
      421 ggccagccag tgcgctcttg cctattgcca gtgagcgcca tcggcgcccg gccggtggcg
      481 acgatcgaag cggtcgggca gacgccgaac ggccacaaga tccagggggc atggctcgat
      541 aaggaagtcg tccagtgcgg ctattgccag tccgggcaaa tcatgtcggc cacggcgctc
      601 ttaaaccaga ctccgcatcc gaccgatgcc gatatcgatg ctgcgatgtc cggcaacatc
      661 tgccgttgcg ggacttatgt ccgaattcgc gaggccatac acgcggccgc gtcggtttag
      721 ggaggcagcc atgggatatg acctgccctc acggcggttt gtgctgaagg ccggcgcggc
      781 agtgggcggt ggcctgttgc ttggcctgca tctgccgagc cttgcgcgcg cgtctgccga
      841 tatgtccagc gactttgagc cgaatgcgtt cctgcggatc gatccgcata atacgatcac
      901 attgatcctg ccgcacaccg aagtcggaca aggaatctat acgagtgccg ccatgctcat
      961 gggcgaagaa ctcgaaatcg gcctggatca ggtcaatatc gaagccgcgc cgccggacat
     1021 caaaaaatat attgatcccc tgcttggtga tcaggcgact ggcggctccg cctcgacacg
     1081 cgcggattgg gtgcgcctgc gcgaggccgc ggctgttgca cgtgtcatgc tgatctcggc
     1141 tgctgcgaaa aagtggaacg tcgatccggc aagctgccgc gccgagcgtg gaatcataca
     1201 tcacgatgca agcggcaaat cagcctccta tggcgacgtc gcgagcgatg ccgcgcaatt
     1261 gccggtgccc tccaatgtca agctgaagga tccgtcgcag ttcaagctga tcggcaccag
     1321 cgccaagcgg atcgatacgc cttccaaggt caacgggact gcactctacg gcatcgacat
     1381 catcgtgccc gatatgcgtt tcgggaccgt cgcgatcaca cccgtgaagg gtggcaaact
     1441 cgccagtatg gatgaggcgg cagcgcgccg cgtgcctggt gtccacgacg tcatccgcaa
     1501 cgatgagggc gatgcaattg ccgtgatcgg cgatcacatg tgggcggcga agcaagggct
     1561 cgaagcgctc aatctcaagt gggacgccgg agagaacggt gatgtcacgc tcgaaacgat
     1621 catctcgacg atggatcggg cgtcgcacaa cgagggcgtc gtggcaaaga aggaaggcga
     1681 tgccgccgcc gcgatcaaag gcgcggccac gcaactttcg gcaatctatc aatcaccgtt
     1741 tctgtcgcat tcgccaatgg agccactgaa ttgcacgctg cacatccggc cggacagcgc
     1801 cgaaatctgg ctcgggacgc aggtgccggt gcgggcacaa aaggcagtcg cgagtgtcgc
     1861 cggcctgccg caagacaaag tgacggttta caatcagctc atgggcggcg cgttcggccg
     1921 gcggctcgat atcgatagcg tcgaagtcgc ggcctcgatc gccaaacatg tctcttatcc
     1981 ggtcaagctc gtatggacgc gcgaggaaga cctgcgccac gattattatc ggccctatta
     2041 ttacgaccgt gtcgccgccg gcctcgatgc gaacgggaaa ctcgtcggcc gcacgcatcg
     2101 cgtcaccggc ccgtctattc tggcacgctg ggcgccgccg gcttttaaaa acggcctcga
     2161 tgacgatgcc gtcttatgcg cggctgagac gccttacgaa attcccaatg aatatgtgga
     2221 ctatgtccgc gacgaaccgc gggcgatgaa cacaagttgg tggcgtggcg ttggcccgac
     2281 gcacaacctc ttcgtcgtcg agagcttcat tgacgaattg gcccacgcgg ccaagcagga
     2341 cccggtcgaa tttcgtcgcg gcatgctcaa gaagaatccg cgagccctcg cggtcctcaa
     2401 tctcgcggcg gagaaatccg gctggggcag tcccctttcg cgcggcaccg gacgtggtgt
     2461 cgaattgcaa ttcgcgttcg gcagctacct ctgctgcgta ctcgaagtcg aagtgacgcc
     2521 gcccggcgag atcagcgtgc gccgcgccgt cgtcgcgatc gattgcggca tgaccgtcaa
     2581 tcccgacact gttcaagcgc aaatccaggg cggtcttatc ctcggtctcg gcacggccat
     2641 gtacaacgag atcacgctga ccggtggcgc aatcgaccag agcaacttcc acgattaccg
     2701 ggcaatgcgc atcaacgaag cgccgaagat cgaagtctat cagatccgca atggcgaaaa
     2761 gcccggcggc atcggcgaga cagggacggc cgccgcggcg ccggccttgg gcaatgcgat
     2821 tttcgccgcc acgggcaaga gattgcgtcg gcttccgttc gggcgcggac aattgagcgg
     2881 aacctagcag acggcaacat aatcccattg tgaggatgga cgctcgcgca tgaaaaaatg
     2941 gctcgctttc gcctttggtc ttgccgcgct cgccggtgtg gccgcggccg tatttttctt
     3001 cttgcccgcc gggctcgaac ccgtggcagc aagcgctgcg cagccgagcg gcgacgcgct
     3061 gattgcgcgt ggcgaatatc tgaccaaagc cgccgattgc gaagcctgcc acacgaatcc
     3121 cggcggcgcg tctttcgccg gcggtcggag gtttgatctg ccgttcggga cactttatgc
     3181 gccgaatctc acgccggatc gcgcgcatgg catcggcgcg tggagcaatg cggaattcgt
     3241 gcgcgccctg caccacggca tcggccgcaa tggcgaagac ctctatccgg catttcccta
     3301 tgcgtcttat gcgttgctat cagacgacga cgcgctggcg atccgggcct atctcgcgac
     3361 cgtcaagccc gtcgcggcga cgccgcctgc gaacgatctg aaattcccgt tcaaccagcg
     3421 ttatctcatg cgggcctgga agctcctgtt cgtcccgggc catcgcttcg aggcggatac
     3481 atcaaaggat gcgacctgga atcgcggcgc ctatctcatc gggccccttg gccattgcgg
     3541 cgaatgccac acgccgcgcg acctgatgca aggactggat gacagccgca aatttgccgg
     3601 cacgaaacag gtcggctggc tcgcctataa tctcaccagc gatcccgacc atggactcgg
     3661 cggctggagc gacgcgcaat tggcgcaata tctctccgtc ggccatgccg aaggacacgg
     3721 accggcgtct gggccgatgg cagaagtcat tggatttagc ttgcgctatc tgaggcccga
     3781 cgatatccgc gcgatggtca cctatctgcg cagcatcccg cctcagcccg acgggccgcc
     3841 tgctgcgggc cgcggcgcgc cagccggcgt acaaaagccc ccgctcggcg cgcgtatctt
     3901 tgtcgaggcc tgcgcgggct gccatctgcc gagcggccac ggccgccaat cgccttgggc
     3961 agctctggcc ggttcgcaca cgaccgccga tcccaacgcc aacaatctgc ttcaagtgct
     4021 ggctcaggga acgcagatta agacttcgca gggcctgatg ttcatgcacg cgttcacgag
     4081 cgcctataca gatgaagagg tggcggcgct cgctaattac acctgcgcgc aatttggtca
     4141 gaccacgtgc catgtcacgg cggatcaggt gcataaagtg cgtggggacg ttccggcgaa
     4201 gacggaagaa gcctcgcaat gatcagcggc tggaaacgat cgagaacgaa ttgcccataa
     4261 tgttcaccgg gacgctgacg acggcgccga gcgcactggc catttgatcc gtctggtcag
     4321 acattcccac atggttcttc tggatcccgc tttggagcac atgctccaac tgcggggcga
     4381 tcgcggcgat ctgtgcatat ttgttgtgat ccgacggatc gtcgccatgc acgttcgtca
     4441 aatcgatgac cacagcgcca agtttcgcca aatcttggac gtttccatcg tcgcccacgc
     4501 gggcctttcc gcctgccaga aactttgaca aggccagagc cttatcatcc ttcgagagca
     4561 caatgtaata aggttttttg ggctttccgt aagcacgcaa atccgatttg aaaacgtcga
     4621 tatcgatgtc cggcgccgca agaagaacca acccgatctt ctccggatgt ttcagaccgc
     4681 cttgcatttt gatctgacgc atagcttcga caaacaccca attgcccatg gaatgcgcga
     4741 ggatgttgac ctcttccgca ttgctgtcag tcagtaagcg aattgtgtgg gcaagctgat
     4801 cgcgcgccgc tgtggcgctg ttattgtcgt agagatagtc cgtcaatttg ccccgcgaag
     4861 cccaggtgaa cagcaccggc acatgatccg tgtgcgcatc gtgaacgacc tgcgtaaagc
     4921 catagagcgc ctcggcaaac atcgtgttga agccgtggac gaagaggaag attttgcgat
     4981 ggccgggcgg acgtttcgcc aattcggcat tgaccgtagc gagaaacgcc cgatctccat
     5041 cgatatagcc agcgtcgcgg accgcgaaat cgcggcgcgg atcgccgggc ggcgtctgcg
     5101 gccattcgat cttcccatcg acatgcgtcg gaggcaccga catggtcgct gacgcgtaat
     5161 cgagccagtc agcgcgaaag ccgctgtaca atgtgcccgg ccgcgcgtcg cgctcacgcg
     5221 tcgaagcgat gagaatcgta tgctcagtcg cgctgggcgc ttcgtaggcc gtggtcgcca
     5281 gaaacccgct atcgggccgc gccgcgcagc ccatcatcag gttcatgcag acgagcacga
     5341 gcgcggctga cagccagcgt cgcaccctca ccctggtcag gcctgagaac aacgagtccc
     5401 cccgagcaaa tgctgccggc ctccaaaacc gaacagcttc aacaacggcg ctcgaaacta
     5461 tgtagggttt gactgccttt gtcacgcgct gacgagacag c
//
LOCUS       DASWOG010000275         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_335961,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000275 DASWOG010000000
VERSION     DASWOG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335961"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..574)
                     /locus_tag="VGB93_05575"
     CDS             complement(89..574)
                     /locus_tag="VGB93_05575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383747.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEY0145610.1"
                     /translation="MHFDAEFWDFVAFIVFAGLLGYFGLHTKIATTLDARAARVKSQI
                     DEAVALRKEAEALLASFVQKKKEAEDEAKAIIAQAQVEAELIAKEAHERASDFVQRRT
                     KQAEDKIASAEMQALDQVRGAAADAAAKASEIVLKSQVKGSYGDELVEKDIDGLKRLL
                     H"
     gene            complement(590..1135)
                     /locus_tag="VGB93_05580"
     CDS             complement(590..1135)
                     /locus_tag="VGB93_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEY0145611.1"
                     /translation="MANDLEHTTATQAQPAPAEEPFPPFDTANFTSLLVWLVLTFGAL
                     YLLMSRIALPRVANILETRHNKINADVLAAHAKRKEADQAAADYQKTLADARTNAQNL
                     AQETHAKLAAEADAKRHALDSELAAKLAVAEKQIEETKAKAMANVGQIAQEATTAILE
                     HITGKPADPAAVADAIAKLKV"
     gene            complement(1207..1437)
                     /locus_tag="VGB93_05585"
     CDS             complement(1207..1437)
                     /locus_tag="VGB93_05585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY0145612.1"
                     /translation="MDPTAARYVGAGLAAIGMGAAAIGVGLIFGHFLSGALRNPTASD
                     GQFGRAFIGVALAEGLGIFAFLVAILLLFVLH"
     gene            complement(1491..2231)
                     /locus_tag="VGB93_05590"
     CDS             complement(1491..2231)
                     /locus_tag="VGB93_05590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0145613.1"
                     /translation="MDPIHQFAVEPFVPVHLFGLDLSFTNASLFMVLVVALVCVVMLI
                     GTAQHAMVPGRLQSAAEMSYEFVASTIRMTNGNEGMKFFPLIFTIFMFVLACNLLGLL
                     PFSFSITSQIVITFALAIMVILIVIGVGIYRHGLRFFKVFAPSGVTPILLPFIVLVEI
                     ISFATRPISLSVRLFANMLAGHITLKVMAGFVVALLGAGAAAVIAPLPFIANVVLLAF
                     ELFVAVLQAYVFAILSCVYLNDAIHPGH"
     gene            complement(2289..>2549)
                     /locus_tag="VGB93_05595"
     CDS             complement(2289..>2549)
                     /locus_tag="VGB93_05595"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0145614.1"
                     /translation="EHRNPDESETMTTPVLSGQSLGAANLGLRVLVEFVSAIIVGSLI
                     GWQIDSWAHTGPIFLIAFLMLGLAAGMLNIYRIAIGPKKPER"
BASE COUNT          547 a          756 c          763 g          483 t
ORIGIN      
        1 tgcctcgcgc ctgcggcgaa acaagcgcgt cggcgcttgc cgcgtttagc catgcgaccg
       61 cgaccttttc aaggccgcgt tgcggacctt aatgcagcag gcgtttaagg ccgtcgatgt
      121 ccttctcgac aagctcgtcg ccataggaac ccttgacctg cgatttgagc acgatctcgg
      181 aagccttggc ggccgcatcc gccgcggcgc cgcgaacctg atcgagggcc tgcatttcgg
      241 ccgaagcgat tttgtcttcc gcctgtttgg tgcggcgctg gacgaaatcg ctggcgcgct
      301 cgtgcgcttc tttggcgatg agttccgcct cgacctgggc ctgcgcaatg atcgccttgg
      361 cctcgtcctc ggcttctttc tttttctgca cgaacgaggc gagcaaagcc tccgcctcct
      421 tgcgcaaggc gacggcttcg tcgatctgcg acttcacccg cgctgcgcgc gcgtcaagcg
      481 tcgtcgcgat tttcgtgtgt aggccgaaat agccgagcag gccggcgaag acgatgaatg
      541 cgacgaaatc ccagaattca gcatcgaaat gcatggcgcc gctccgctct caaaccttga
      601 gtttcgcaat agcatcggca acggccgcag ggtccgccgg cttgccggtg atatgttcga
      661 gaatggccgt cgtcgcctcc tgcgcgatct gcccgacatt ggccatcgct ttggctttcg
      721 tctcttcgat ctgcttttcc gcaacggcca gtttcgccgc aagttcgcta tcaagcgcgt
      781 ggcgcttagc gtccgcctcg gcggcgagct tggcgtgcgt ttcctgcgcc aggttttgcg
      841 cattcgtgcg cgcatcggcg agagtcttct gataatccgc cgcggcctga tcagcctcct
      901 tgcgcttggc atgcgccgcc agcacatcgg cattgatctt gttatggcgg gtctcaagaa
      961 tattcgcgac acgcggcagc gcgatacgcg acatcaagag atagagcgcg ccgaaggtca
     1021 gcacgagcca caccagcaac gaggtaaaat tagccgtatc gaatggcgga aacggctctt
     1081 cggccggagc cggctgagcc tgcgtggctg tcgtatgttc gagatcgttt gccattcgaa
     1141 atttccgatc caagccctaa acattaaaga cagacgcggg ccgtacgcga cccgcgccgt
     1201 ttcaaatcaa tgaagcacga acaacagaag gatcgcgacc aagaaggcga aaatgccaag
     1261 gccttctgcc agcgcgacgc cgatgaatgc acggccgaac tggccgtcag acgcggtcgg
     1321 gttgcgcagc gcgccagaga ggaagtggcc gaagatgagg ccaacgccga tagcggcggc
     1381 acccatacca atcgccgcaa ggccggcgcc gacgtatcga gctgctgtgg gatccatttt
     1441 atgctcctga caattttgaa gggcgaccga tctgttgttg cgctctttat tcagtggccc
     1501 ggatgaattg cgtcattcaa atagacgcaa gagaggatgg cgaaaacata agcctgtagt
     1561 accgccacga agagttcgaa cgccagaagg acgacgttcg cgataaaggg caggggcgcg
     1621 atcaccgccg cggcgccggc gccgagcagt gcaacgacga agcccgccat aactttcagc
     1681 gtaatgtgcc ccgcgagcat gttggcgaag agacgaaccg acaggctgat gggccgcgtc
     1741 gcgaaggata tgatctcaac gagcacaatg aacggcagca ggatcggcgt cacgcccgaa
     1801 ggcgcgaaaa ctttgaaaaa gcgcagcccg tgccggtaga tgcccacgcc gatcacgatc
     1861 aggatcacca tgatggcgag ggcgaaggtg ataacgatct ggctggtgat cgaaaagctg
     1921 aacggcagca ggcctaggag attgcaggcc agcacgaaca tgaagatcgt gaagatcagc
     1981 gggaagaatt tcatgccttc gttgccgttg gtcatgcgga tcgtcgaggc gacgaattcg
     2041 taagacattt cggcggccga ctgcaggcgc cccggaacca tcgcatgctg tgccgtgccg
     2101 atgagcatga cgacgcaaac gagcgcgacg accagcacca tgaacagcga tgcattggtg
     2161 aagctgagat cgagcccgaa aagatgcaca ggcacgaaag gttcgacggc aaattgatga
     2221 ataggatcca ttgctaaacc cgcaccctca cgtctcgacc cgcgccgtcg cgccggctca
     2281 gctgtttctt agcgttccgg ctttttcggc ccgatcgcga tccgataaat gttcagcatc
     2341 ccggccgcta gccccagcat gaggaaagcg atcagaaaaa tcgggcccgt atgcgcccag
     2401 gagtcgatct gccagccaat gaggcttccg actatgatcg cagagacgaa ctcgacgagc
     2461 acgcgaagtc cgagattcgc tgcgccaagc gattggccgg agagaaccgg cgtcgtcatc
     2521 gtctccgatt cgtccggatt tcgatgctc
//
LOCUS       DASWOG010000276         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_42499,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000276 DASWOG010000000
VERSION     DASWOG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42499"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="VGB93_05600"
     CDS             <1..2529
                     /locus_tag="VGB93_05600"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY0145615.1"
                     /translation="AARSLVIAAVGGYGRATLAPGSDIDLLFLLPSDDDNWGKRVTES
                     VLYLLWDLKQKVGHSTRSVDECLVQSRRDMTVRTALLEARFILGDEKLFADMYSRFDK
                     EIVQHNPREFVVAKLAERDTRISKEGQSRYLVEPNVKESKGGLRDLNSLFWIAKYVYR
                     MREASDLVAAGLFTPRQYRRFLRCEEFLWLVRCHMHFLAGRPEEILSFDMQRPIAERL
                     GYAGRGGLSGIERFMKHYFLIAKEVGDLTAVLSAALEERHAKPPAMLDRFHIRIRRQD
                     KRITSMGFAVENGRLKAARPDVFTNDPVNLIRMFWLADRDSVALHPDLAHLAISHLYL
                     IDAQLRENPEANRLFLDILSSRKSPEAVLRLMNEAGVLGRFVPPFGRIVAMMQFNMYH
                     HYTVDEHLLRALGFLADIDAHRHRDDLPLTDTIISSIEKRRALYVAVFLHDVAKGRME
                     DHSIAGAAVARHLCPRLGLTPAETETVVWLIENHLVMSDTAQRRDLADRRTIETFAAR
                     VQTIERLKMLFILTVCDIQAVGPGVWNGWKGELLRTLFLETQIVLAGGHSKTERTERI
                     EKAKAELHAQLPAWSDTEFDAYASRFPQAYWLRVDPPRKIRHAQLLTRCAANRLEPII
                     DVQTDAFRAVSEVTLIAPDHPRLLSIVAGACAAAGANIVDAQIFTTNDGMALDSFFVS
                     RTFERDDDEIRRGERIALAIEQALRGQIRLADLVAAKSAAAAAKGTPFSVPPQVSIDN
                     ELSNRYTVIEVSGRDRVGLLYSITDILSRLNLNIGSAHIVTFGEKAADVFYVTDLTGA
                     KITTSGRRASIRQKLLDAFPSRGPGAAKPMPVAPSNAGGDGATA"
     gene            2620..3192
                     /gene="grpE"
                     /locus_tag="VGB93_05605"
     CDS             2620..3192
                     /gene="grpE"
                     /locus_tag="VGB93_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0145616.1"
                     /translation="MPDETLPETNFSAADGEAPSTAEGNQDPFVVLQNLQRENGELKD
                     RLLRALADMENMRRRTEKEVADAKLYGVSNFARDMLSFADNLRRAVENIPDELHKSTD
                     SAITTLIGGIEVIERDFISRLARHGARKMEPLGTKFDANLHEALYEVPDETVPNGTVV
                     KVVEDGYTIGDRMLRPAKVGISRGGTKSAR"
     gene            complement(3214..3822)
                     /locus_tag="VGB93_05610"
     CDS             complement(3214..3822)
                     /locus_tag="VGB93_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY0145617.1"
                     /translation="MMQKDREKNDDRQRDTDQPKKQTFSETHDPSSFRIWRNNGVAAV
                     SVPCAGSRGSAELFKERIVSPKGELRCECSMKDSFSRFAAWCAHIAGHPMAFVISTLL
                     IAIWAVLGPIYHYSDTWQLVANTATSIITFLMVFVIQNSQNRDGAAIQAKLDELIRSG
                     NAENRFVGIDLLTEDEVGKLRERSAARAKQDTRADNVTGQDV"
     gene            complement(3889..4521)
                     /locus_tag="VGB93_05615"
     CDS             complement(3889..4521)
                     /locus_tag="VGB93_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0145618.1"
                     /translation="MTLFAAQRETFADGLIYVPGYLADAEQRALLRDIEAVIAAAPLF
                     QPRTPKTGKPFSVEMTNCGPLGWVSDRDGGYRYQAMHPETGLPWPPIPELALRAWNEL
                     GAYAHAPEACLVNVYGPAAHMGLHQDKDEMHLGAPVVSLSLGESCVFRWGGTSRGGPT
                     QSIVLESGDALVLAGKARLAYHGVPRISKGTSDLLPGRRINLTLRRVTNP"
     gene            complement(4518..4901)
                     /locus_tag="VGB93_05620"
     CDS             complement(4518..4901)
                     /locus_tag="VGB93_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEY0145619.1"
                     /translation="MLRFTDHHEWLRLDGDIALIGVTSYAAHEAGTFISAELPKLGAI
                     VKKGERVALLVSVTIAFEVMAPVDGEIIEVNTEVIAVPSRVNADPQGSGWLFRLCIDD
                     AALLDGLMDEDAYTQFAATGQRSVP"
     gene            complement(4901..5536)
                     /locus_tag="VGB93_05625"
     CDS             complement(4901..5536)
                     /locus_tag="VGB93_05625"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0145620.1"
                     /translation="MQRLLVLVRHGQSEWNLRNLFTGWKDPGLTARGVAEARHTGGAL
                     KAEGLGFDKAFTSELSRAQKTLGLILEMLGTPDVTTLRDLALNERDYGDLSGLNKDEA
                     RQRWGEEQVHLWRRSYDVPPPGGESLKDTIARVLPYYCQKILPCVLRGKRTLVVAHGN
                     SLRALLMVLDQLTPDTILDAEIATGAALIYHLNPDATVATKKVLSPHIDLL"
BASE COUNT         1148 a         1730 c         1624 g         1105 t
ORIGIN      
        1 gcagcccgga gcctcgtgat cgcagctgtc ggcggctatg gtcgcgcgac gctggcgccc
       61 ggctccgaca tcgatctgct tttcctgttg ccctcggacg acgacaactg gggcaagcgg
      121 gtgaccgaat ccgtgctcta tctgctctgg gacctgaagc agaaggtcgg ccattcgacc
      181 cggtcggtcg acgaatgcct cgtccagtcg cggcgcgaca tgacagtacg caccgcgctg
      241 ctcgaagctc gcttcatcct cggcgacgaa aagctttttg ccgatatgta cagccgcttc
      301 gacaaggaaa tcgtgcaaca taatccgcgg gaattcgtcg tcgccaagct cgccgagcgc
      361 gatacaagga tttccaagga aggccagtcg cgctatctcg tcgaacccaa cgtcaaggaa
      421 agcaaaggcg gcctgcgcga tctgaactca ctgttctgga tcgccaaata tgtctatcgc
      481 atgcgcgagg cgtcggatct cgtcgcggcg gggctattca cgccgcgcca atacaggcga
      541 tttctgcggt gcgaggaatt tctctggctc gtgcgctgcc acatgcattt tctggccggc
      601 cgccccgagg aaatcctgag cttcgacatg cagcgcccca tcgccgaacg gctgggctat
      661 gcgggccgcg gcggcctgtc cggcatcgag cgtttcatga aacattactt cctcattgcg
      721 aaggaggtcg gcgacctgac ggctgtgctg agcgcggcgc tggaagaacg ccacgccaag
      781 ccccccgcca tgctcgaccg gttccatatc cggatacgcc gtcaggacaa gcgcatcaca
      841 agtatgggct ttgccgtcga gaacggacgg ctgaaagcag cacggccgga tgtcttcaca
      901 aatgacccgg tcaacctcat ccgcatgttc tggttggcgg accgggatag tgtcgcgctg
      961 catccagatc tcgcgcacct tgccatttcg catctttatc tgatcgacgc gcagctgcgc
     1021 gaaaaccccg aggcgaaccg tctttttctc gacattctct catcgcgaaa atcgccggaa
     1081 gcggtgttgc ggttgatgaa cgaagcgggc gtgctcggcc gcttcgttcc gcctttcggc
     1141 cgtatcgtcg cgatgatgca attcaacatg tatcatcatt acacggttga cgagcatctg
     1201 ctgcgcgcct tgggttttct tgccgacatc gacgcgcacc gccatcgcga cgatctgccc
     1261 ttgaccgata cgatcatatc gtcgatcgaa aaacgtcggg cgctctatgt cgctgtcttc
     1321 ctgcatgatg tcgccaaggg tcggatggag gatcattcga tcgcgggcgc tgctgtcgcg
     1381 cgccatcttt gtccccgcct cggtctgacg ccggcagaga cagagacggt cgtctggctg
     1441 atcgaaaatc acctcgtcat gtcggatacg gcacagcgac gcgatcttgc cgaccgccga
     1501 acgatcgaga ctttcgcggc ccgcgtacag acgatcgaac ggctgaagat gctgttcatt
     1561 ttgacggtct gcgacatcca agccgtcggc cccggcgtct ggaatggctg gaagggtgag
     1621 ctgctgcgca cgctctttct cgagacgcag atcgtcctcg ccggcggaca cagcaagacc
     1681 gaacggaccg agcggatcga gaaggccaag gccgaattgc acgcgcaatt gccggcttgg
     1741 tccgacaccg aattcgacgc ttatgcgtcg cgctttccgc aggcctattg gctgcgggtc
     1801 gatccgccgc gaaaaatccg tcatgcgcaa cttctgaccc gctgcgcggc gaatcggctc
     1861 gaaccgatca tcgatgtgca gacggacgcc ttccgcgccg tgagcgaggt cacgctcatt
     1921 gcgcccgacc atccgcgttt actctcgatt gttgccggcg cctgcgccgc ggccggggcg
     1981 aatattgtcg atgcgcaaat tttcacgacc aatgacggca tggcactgga ttcatttttc
     2041 gtttcgcgaa ccttcgaacg tgatgacgat gaaattcgcc ggggcgagcg gattgctttg
     2101 gcaatcgagc aggccttgcg tggccagatc cgcctcgccg atctcgtcgc ggccaaaagc
     2161 gcggcggcgg cggccaaggg gacgcccttc agcgtcccgc cgcaggtttc gatcgataac
     2221 gaattgtcga atcgttatac cgtcattgaa gtctcgggcc gcgacagggt gggcctgctc
     2281 tattcgatca cggatattct ctctcggctg aacctcaaca ttggctcggc ccatatcgtg
     2341 acctttggcg agaaggccgc cgacgtgttc tacgtgaccg acttgaccgg cgccaaaatc
     2401 accacgagcg gccggagagc ctccatccgc cagaagctgc tcgatgcctt ccccagccgc
     2461 ggccccggcg cggccaaacc gatgccggtt gccccttcca acgccggcgg agacggggca
     2521 acggcttgat tttcgccatc cgaacgctga tatcggcgtt aattccagaa ttgtgcgtcc
     2581 cagcgcatcc acccatcgat tgacgagtaa gcggacccca tgcccgacga aacattaccc
     2641 gaaaccaatt tttccgcagc ggatggcgaa gcgccgtcaa cggcagaggg caaccaggat
     2701 cccttcgtcg tcctgcaaaa tctgcagcgc gagaacggcg aactgaaaga ccggctgttg
     2761 cgcgcgcttg cggacatgga aaacatgcgc cgccgcaccg aaaaggaggt ggcggacgcc
     2821 aaactctacg gcgtctccaa cttcgcccgc gacatgctga gcttcgccga caatctgcgg
     2881 cgcgcggtcg agaacattcc cgacgaattg cacaaaagta cagatagcgc gatcacgaca
     2941 ctgatcggcg gcatcgaagt catcgagcgc gactttatct cgcgcctcgc acgccacggt
     3001 gccaggaaaa tggagccgct cggcaccaag ttcgacgcca atctgcacga ggcgctttat
     3061 gaggtgcccg acgaaacggt tccgaacggc accgtcgtca aagtggtgga ggatggctat
     3121 accatcggcg atcgcatgct gcggcccgcg aaagttggca tttctcgcgg cggcaccaaa
     3181 tcggcaaggt aatccaaacg acgcggcagg actttagacg tcctgccctg tgacattgtc
     3241 ggcgcgcgta tcctgtttgg cgcgcgccgc gcttctttcg cgcaatttgc cgacctcgtc
     3301 ctcggtgaga agatcgatgc cgacaaatct attctcggca ttgccagaac gaatgagctc
     3361 gtcgagcttg gcttggatcg ccgcgccatc gcggttctgc gaattttgaa tcacaaatac
     3421 catgaggaac gtgatgatgc tcgtcgcggt attggcgacg agctgccacg tatccgaata
     3481 atgatagatg ggcccaagca ccgcccagat ggcaatcaat agcgtagaaa ttacgaacgc
     3541 catgggatgg cccgctatat gcgcgcacca cgcggcaaat cttgaaaatg aatctttcat
     3601 cgaacactca cagcgtagct cacccttcgg cgaaacgatc ctctctttaa aaagttccgc
     3661 tgagccccgt gagcctgcac acggaaccga aacggcagcg accccgttat tccgccatat
     3721 tctgaaggag gacggatcat gggtctcgga aaaggtttgc ttctttggct gatcggtatc
     3781 ccgctgccga tcatcattct tctcgcgatc tttttgcatc attaagccga cgtgccatgg
     3841 cacagcgatc tctggatcgc tgtgccatcg tgcttaggag aagttgatct agggatttgt
     3901 cacgcgtctg agcgtaagat tgatacggcg tccgggaagc agatcggacg ttcccttcga
     3961 gatacgtggg acgccgtgat aggcgaggcg cgcttttccc gctaacacca aagcatctcc
     4021 ggactcgagg acgatggatt gcgtcggtcc accacgcgac gtcccgcccc aacggaaaac
     4081 gcaggattcg ccgagcgaca gcgagacgac cggcgcccct aaatgcattt catccttatc
     4141 ctgatgcaat cccatatgcg cggccggccc gtagacattc acgaggcagg cttccggcgc
     4201 atgcgcgtac gcgccaagtt cgttccaggc cctcagcgca agctccggaa ttggcggcca
     4261 tggcagaccg gtttcgggat gcattgcctg atagcgataa ccgccatctc gatccgacac
     4321 ccaaccgagc ggcccgcagt tcgtcatctc gaccgaaaat ggcttgcccg ttttcggcgt
     4381 ccgtggctga aacaaaggtg cagcggcaat tacggcttcg atgtctcgca ataaagcccg
     4441 ctgctcggca tcagccaaat agccgggcac atagataagt ccgtccgcaa aggtttctcg
     4501 ctgcgcggcg aacagcgtca cggcaccgac cgctggcctg ttgcagcaaa ttgcgtgtag
     4561 gcatcttcgt ccatcaatcc atcaagtagg gccgcgtcat cgatgcagag acggaagagc
     4621 cagccgctac cttgcggatc ggcgttgacg cgcgacggca cggcaatgac ctcggtattc
     4681 acttcgatga tttcgccatc gaccggcgcc atgacctcga atgctatcgt gaccgagacg
     4741 agaagcgcga cccgctcgcc cttctttacg atcgcgccga gcttgggcaa ttcggcggag
     4801 ataaaagtcc cggcctcatg cgccgcatag gaggtgacgc caatcagagc aatgtcacca
     4861 tcgagccgca accattcatg gtgatcggtg aaccgcaaca tcagagtaaa tcgatatgcg
     4921 gtgacaggac tttcttcgtt gccaccgtcg catctggatt gagatggtag atcaacgccg
     4981 cgccggttgc gatttcggcg tcgaggatcg tgtcgggggt cagttgatcg agcaccatga
     5041 gcaaagcgcg cagggaattg ccgtgcgcga cgacgagcgt gcgtttgcca cgcagaacgc
     5101 aaggcaaaat cttctgacaa taatagggaa gaacgcgggc gatcgtgtcc ttgaggcttt
     5161 cgccacccgg cggcggcacg tcgtaggagc ggcgccacag atgcacctgc tcttcgcccc
     5221 aacgctggcg tgcctcgtcc ttgttgaggc cggagagatc gccatagtcg cgctcgttca
     5281 gcgccaggtc gcgaagggtc gtcacgtccg gcgtcccaag catttcgagg atcagtccga
     5341 gtgtcttttg cgcgcgcgaa agttcggagg tgaaggcttt atcgaaaccc aacccctctg
     5401 ccttcaacgc gccgccggtg tgtcgcgcct cggcgacgcc gcgggccgtc aaccccggat
     5461 ccttccagcc ggtgaacagg ttcctgagat tccattcgct ctggccgtgc cgcacgagaa
     5521 cgagaagccg ctgcatgaaa ggttcctact tcaagccgag cacgtcggcc atcgaataga
     5581 ggccgggctt gcgcccgcgc gcccaga
//
LOCUS       DASWOG010000277         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_171183,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000277 DASWOG010000000
VERSION     DASWOG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171183"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3430
                     /gene="mfd"
                     /locus_tag="VGB93_05630"
     CDS             <1..3430
                     /gene="mfd"
                     /locus_tag="VGB93_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0145621.1"
                     /translation="IADFSRALAPTAEERAVVLVHIARDAQRARAFNETLAFVAPEIE
                     ILDFPGWDCQPYDRVSPNAAISARRMTVLSRLARSRTAAGRPRILSTTINAVLQRVPP
                     REKVAAESFSAAPGNAVNMTSLTEWLENNGFARASAVRDTGEYAVRGGILDLFAPSTL
                     EPIRLDFLGDTLESIRTFDPETQRTVAPLRAVDLVPVSEVQLTTDSMRRFRQGYVGAF
                     GGGLRGDTLYEAVSEGRRHPGLEHWLPLFYDGLDTLFDYIGGAPLVLDPLTDEAATER
                     LEQIRDYYDARKHAHDSDPSNSAYKPLPPEALYLPVDEWTKQLAAQGTARVTAFSPPE
                     GGKTLVIDCGGKLGRNFVAERADEGANVFHAAAEHVQALQAQGKRVVVAGWSEGSRER
                     LGHVLKDFGLRAVEPVSSFADALRASKGTVALAVIGLEAGFEAPDLAIVGEQDILGDR
                     LIHARKKARRAQDFIAEVGVLSAGDLVVHVGHGIGRFQGLTPVEAAGAPHDCLEIHYA
                     GGDKLFLPVENIELLSRYGSEGSDVELDRLGGAGWQKRKARMRKRVLEMAAGLLRIAA
                     ARQTQAAPRLMPPEPLYEAFCAGFPYDETEDQDAAINAVLDDLASGPQMDRLICGDVG
                     FGKTEVALRAAFAAAINGRQVAVVVPTTSLARQHAKNFAARFAGLPVQVAQLSRMVGS
                     ADTKVVKAGLTSGDIDIVVGTHALLGGQIAFRDLGLVIIDEEQHFGVKHKERLKELRA
                     EVHVLTLSATPIPRTLQLALTGVRELSIIATPPVDRLAVRTAISPFDALLVREALLRE
                     RYRGGQSFYVCPRIEDIEEAGAFLRQNVPEAKFIIANGQLPASELEARMSAFYDGKYD
                     ILLSTETVQSGLDIPRANTLIVHRADMFGLAQLYQLRGRVGRAKTRAYAYFTTPMNKK
                     MTPQAEKRLGILQSLDTLGAGFQLATHDLDIRGAGNLLGDDQSGHIKEVGYELYQQML
                     EEAVLALKSGIDEPVEEAWSPAITLGTPVTIPEDYVADLDLRLGLYRRLSTLQTDAEI
                     EAMAAEMIDRFGPLPRAVEQLLALVSIKALCLKANVEKVDAGPKGVTLAFRDNQFANP
                     QALVRYVAEQGPQGKVRPDMRVVFIRDFENLPERLEGTRTILRALAAMATKKAA"
     gene            complement(3439..3957)
                     /locus_tag="VGB93_05635"
     CDS             complement(3439..3957)
                     /locus_tag="VGB93_05635"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HEY0145622.1"
                     /translation="MAGVIHFAFRNFTLTLFLVGLIASFIALARAPRPLSSAAIIEAM
                     FSYFLLFSIGVSFFYNFVIHTFFGAIAARFIGWADSPFQAEVGFASLGFAAVGVLAFR
                     GSFGLRLAAVVGPSVFLLGAAAGHIYQIAKTQNFAPGNAGVILYSDVLIPVIGLAFLW
                     LQSRARPKGRAQ"
     gene            complement(3981..>5167)
                     /locus_tag="VGB93_05640"
     CDS             complement(3981..>5167)
                     /locus_tag="VGB93_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY0145623.1"
                     /translation="ILDLLNGNGEEARLVGGAVRNALLGKPVSEFDIATTATPETVAA
                     RAQAANLRAVPTGLAHGTVTVIIDGHPFEVTSLREDIETDGRHAVVRFSRDFAADAQR
                     RDFTMNALSLSREGVLYDYVGGLDDIAAKRLRFIGDPAARIKEDYLRVLRFFRFSAGY
                     AEGPLDTEGLLACIRARDGLARLSRERVRNEVLKLLAAPRGGEITREMSQAGLLGPLI
                     ASAPNPRRLENLLNLRSGEAADPVLNLAALCLNLPEDAHRLREKLRLSNPEHQRLVKA
                     ADALETLHDFDTPPDERRLLALLFRYGRRGACDALLLAASQARDAADLWRKALDFLRD
                     AKEPRLPFGGNDLLARGIKDGKAVGETLRLLETRWIEAGFPDDPTKLKIMLDEATQSH
                     RD"
BASE COUNT          973 a         1588 c         1643 g          963 t
ORIGIN      
        1 cattgccgac ttcagccggg ctctcgcgcc gacggccgaa gagcgcgccg tcgtgcttgt
       61 ccatatcgca cgcgacgcgc agcgtgcccg cgccttcaat gaaacgctgg ctttcgtggc
      121 gcccgagatc gagattctcg attttcccgg ttgggattgt cagccctacg atcgcgtctc
      181 gcccaatgcg gcgatctcgg cgcgtcggat gacggtactg tcgcggctcg cacgttcgcg
      241 caccgcggcc ggacggccgc gcattctctc gacgacgatc aacgccgttc tgcaacgtgt
      301 gccgccgcgc gaaaaagtgg cggcggaatc cttttccgca gcgcccggta atgccgtcaa
      361 catgacgagc ctgaccgaat ggcttgagaa caatggtttc gcgcgcgcct ctgcggtgcg
      421 cgacacgggt gaatatgccg tgcgcggcgg catcctcgat ctttttgcgc cgtcgacgct
      481 ggagcccatc cggctcgatt tcctcggcga tacgctcgaa tcgatccgca cattcgaccc
      541 ggagacacaa cgcaccgttg cgccgcttcg cgcggtcgat cttgtgccgg tcagcgaagt
      601 gcaactgacg acggactcca tgcgtcgctt ccgccagggc tatgtcggcg ctttcggcgg
      661 cggcctgcgc ggcgatacgc tttacgaggc ggtgagcgaa gggcgccggc atcccggcct
      721 cgaacattgg ctgccattgt tctacgatgg gctggatacg ctgttcgatt acattggcgg
      781 cgcaccgctg gtgctcgatc cgctgactga cgaggctgcc accgaacgcc tcgagcaaat
      841 ccgcgattat tacgatgcgc ggaaacacgc gcacgatagc gacccgtcca attcagccta
      901 taagccgctg ccgccggagg cgctgtatct gccggtggac gaatggacga aacaacttgc
      961 ggcgcaaggc accgcgcgcg tcacggcttt ttcgccgccg gaaggcggca agacgctcgt
     1021 catcgattgc ggcggcaaac tcggacggaa tttcgttgcc gagcgcgccg acgagggcgc
     1081 aaacgttttc cacgctgcgg ccgaacatgt tcaggcgctc caggcgcaag gcaaacgcgt
     1141 cgtcgtcgcc ggctggtcgg aaggttcgcg cgagcgtctc ggtcatgtgc tgaaggattt
     1201 cggcctgcgt gcggtcgagc cggtgtcgtc tttcgccgat gcgctgcgtg cgtcgaaagg
     1261 aaccgtcgcg ctcgccgtca ttgggcttga ggccggattc gaagcgccgg acctcgccat
     1321 cgtcggcgag caggacattc tcggcgaccg tctgattcat gcccgcaaga aagcccgccg
     1381 cgcgcaggat ttcattgccg aagtcggtgt gctttcggcc ggcgatcttg tcgttcacgt
     1441 cggccatggc atcggccgct ttcagggcct caccccggtc gaggccgcgg gcgcgccaca
     1501 tgattgtctc gaaatccatt atgccggtgg cgacaagctc ttcctgccgg tcgagaacat
     1561 cgagcttttg tcgcgctatg gttctgaagg ctcggatgtc gaactcgaca ggttgggcgg
     1621 tgccggctgg cagaagcgca aggcgcggat gcgcaagcgc gtgctcgaaa tggccgcggg
     1681 cctgctgcgc attgccgccg cgcggcagac gcaggcggcg ccgcgcttga tgccgcccga
     1741 gccgctttat gaagcgttct gcgccgggtt cccctatgac gagacggaag accaggatgc
     1801 cgcgatcaat gcggtgctcg acgatttggc gtcaggcccg cagatggacc ggctgatctg
     1861 cggcgacgtc ggcttcggca agacagaagt tgcgctccgc gctgcgttcg ccgcggctat
     1921 caatggcagg caggtcgccg tcgtcgtgcc gaccacatcg ctcgcacgcc agcacgcaaa
     1981 gaatttcgcc gcgcgcttcg cgggtctgcc ggtgcaagtg gcgcagctct cgcgcatggt
     2041 tggctcagcc gatacaaagg tcgtcaaggc cggcctcaca tcgggcgaca tcgacatcgt
     2101 tgtcggcacg cacgctttgc tcggtggtca gatcgccttc agagatctcg gcctcgttat
     2161 catcgacgaa gaacagcatt tcggcgtcaa gcacaaagag cggctgaagg aacttcgcgc
     2221 cgaagtgcat gtgctgacgc tttcggcgac gccgatcccg cgcacattgc agcttgcgct
     2281 gacgggtgtg cgcgaactct cgatcatcgc gacgccgccg gttgaccggc ttgccgtgcg
     2341 cactgcgatc agccccttcg atgcattgct ggtgcgcgaa gcgctgctgc gcgaacgtta
     2401 tcgcggcggc cagagttttt acgtctgccc aaggattgag gacatcgaag aggccggtgc
     2461 cttcctgcgc cagaacgtgc cggaggcgaa attcatcatc gccaacgggc agcttccagc
     2521 ctccgaactc gaagcgcgaa tgtcggcatt ctacgacggc aagtatgaca ttctgctctc
     2581 gacggagacc gtccagtctg gcctcgacat tccgcgcgct aacacgctca tcgtccatcg
     2641 cgccgatatg ttcggcctcg cgcagctcta tcaattgcgt ggccgcgtcg gacgcgcgaa
     2701 gacgcgtgcc tatgcctatt tcacgacgcc gatgaacaag aaaatgacgc cgcaggcgga
     2761 aaagcggctc ggcatcctgc aatcgctcga tacgcttggc gcgggcttcc agcttgcgac
     2821 acatgatctc gacatccgcg gcgctggcaa tctgctgggc gacgaccagt cgggtcacat
     2881 caaggaagtc ggctatgagc tttatcagca gatgcttgag gaggcggtgc tggcgttgaa
     2941 gtcgggcatc gacgagccgg tggaagaagc ctggtcgccg gcgatcacgc tcggtacgcc
     3001 tgtgactatt cctgaggatt atgtcgccga tctcgatctg cggctcggtc tctatcgtcg
     3061 cctttcgacg ctccaaacgg atgccgagat tgaggcgatg gcggccgaga tgatcgaccg
     3121 cttcgggccg ctgccgcgtg cagtcgagca attgctagcg cttgtttcga tcaaggcgct
     3181 ttgcctcaag gcgaatgtcg aaaaggtcga tgcggggccg aagggcgtga cgcttgcgtt
     3241 ccgtgataat cagttcgcca atccgcaagc cctcgtccgc tatgtcgcgg agcaaggccc
     3301 gcaggggaaa gtgcgtcccg acatgcgcgt cgtgtttatc cgcgatttcg agaatctgcc
     3361 cgagcgactc gaagggacac gtaccatcct gcgcgctctc gccgcgatgg cgacgaagaa
     3421 ggcggcgtga caacgcggct attgtgcgcg tccttttggc ctcgcgcgcg attgcagcca
     3481 aagaaacgcc aagccgataa ctggaatgag tacgtcgctg tacagtatca cgccggcgtt
     3541 gcccggcgcg aagttttgtg ttttggcgat ctggtagata tgtcctgcag cagcgccaag
     3601 cagaaagact gatgggccaa caacagctgc aagtcgcaat ccgaaacttc cgcgaaacgc
     3661 gagaacgccg acggcggcga agccgagact cgcgaagccg acctcagctt gaaacgggct
     3721 atcggcccac ccgatgaatc gcgcggctat tgcaccaaaa aacgtatgaa tgacgaaatt
     3781 gtagaaaaag cttacgccga tcgaaaacag caaaaaatag gaaaacatgg cctcgataat
     3841 agcggcgctg cttagcggcc tgggcgcgcg ggcaagcgcg ataaacgacg ctataagacc
     3901 caccaaaaat agggtgagag tgaaattcct gaacgcaaag tggatgacac ccgccattgg
     3961 ggcagccttc tgaaggacgg ttaatcgcgg tgcgactgtg tcgcctcgtc gagcataatc
     4021 ttcagctttg tgggatcgtc gggaaaccct gcctcaatcc agcgcgtttc taaaagtctc
     4081 agcgtttcgc cgacagcctt gccgtctttg atgccgcgcg cgagaagatc gttaccgccg
     4141 aagggcagtc gcggttcttt cgcgtcccgc agaaaatcga gcgcttttcg ccacagatcg
     4201 gcagcgtcgc gagcttgcga cgcggcaagg agcagggcgt cgcaggcgcc ccggcggcca
     4261 tagcgaaaca gcagcgctaa aagcctgcgc tcatccggcg gcgtgtcgaa atcatgcagc
     4321 gtttcgagcg catcggcagc cttcacgaga cgctgatgtt cgggattcga gaggcgaagc
     4381 ttctcgcgca ggcgatgggc gtcttcgggc agattgaggc agagcgcggc aagattcaga
     4441 acgggatcgg ccgcttcgcc ggatcgcaga ttcaacaaat tctcgaggcg tcttgggttg
     4501 ggtgccgagg cgatgagagg gccaagcagg cctgcttgcg acatctcgcg cgttatctcg
     4561 cccccccgag gcgcagcgag aagcttgagc acttcgttgc gcacgcgctc gcgcgacagt
     4621 cgcgccagac cgtcgcgggc gcggatgcag gcgagcagcc cttccgtatc gagaggccct
     4681 tcggcatacc cggccgagaa gcggaaaaag cgcagaacgc gcagataatc ttctttgatc
     4741 cgcgccgccg gatcgccaat gaagcgcagt cttttcgcgg cgatgtcgtc gagaccgccg
     4801 acataatcgt agagcacacc ttcgcggctc agcgacaaag cgttcatcgt gaagtcgcgt
     4861 cgctgcgcgt cggcggcaaa atcgcggctg aagcgcacga cggcgtggcg gccatccgtc
     4921 tcgatatcct cgcgcaggct cgtcacctcg aacggatggc catcaatgat gacggtcacc
     4981 gtgccgtgcg cgaggccggt cggaacggcg cgcaaattcg cggcttgcgc ccgtgcggcg
     5041 accgtctccg gcgttgctgt cgtggcgatg tcgaattccg agaccggctt gccgagcagg
     5101 gcgttgcgga ccgcgccgcc gacgaggcga gcctcctcgc cgttgccgtt gagcaggtca
     5161 aggatgc
//
LOCUS       DASWOG010000278         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_354316,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000278 DASWOG010000000
VERSION     DASWOG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354316"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGB93_05645"
     CDS             <1..504
                     /locus_tag="VGB93_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145624.1"
                     /translation="SVPDYDVIFVVDADEAFGDYLLYNSTDPHPVVGTQGLVAVAWHP
                     AFTEFSGKEMQRHFLRLAHRTMTERDYEAWLGVRIFGEAMLRTNATDTKSLRDYLLSD
                     KFTIAAFKGQGLSFRRWDLQLRQPILLAGPRALVSIAPIDGFLHPRFNTDTLGYDEPE
                     TKCHAVE"
     gene            491..1480
                     /locus_tag="VGB93_05650"
     CDS             491..1480
                     /locus_tag="VGB93_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEY0145625.1"
                     /translation="MRLNKYIGAARGLAITSGVLMLVATPCFAAKLFITSERDNTVTV
                     LDSNTLTVKKVINVGTRPRGIAITPDYREIFVCIGDDDRMDVIDTKTLAVSRSVPNVL
                     DPELVAIDPVGKRVYAANEEDSQVTVIDRASGTIVGTVGVGAEPEGLSISPDLSTVVN
                     TSELTSMAHFINTKTLTIEDNVLVDQRPREAQYTHDGKQVWVSAEVGGTVSVIDAQKR
                     TIIKKIEFDIQGVQPELIQPVGIVFSKDGKLAFVALSHANRIAVVDTSTLSPTSYILV
                     GERPWHMALDPDGSKLYVANGNTNDMTVIDVASLKAVRSVNVGHLPWGVVAMP"
     gene            complement(1511..2131)
                     /locus_tag="VGB93_05655"
     CDS             complement(1511..2131)
                     /locus_tag="VGB93_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145626.1"
                     /translation="MKLLIVDDHVVVRAGVRRLFAALPGTTVFEAGSTHEALSLYRSE
                     TPDVLLLDLNLPNSSGFELLRRLLIENRNVKVVVLSMHAEPIYVSRTLNAGARGYVSK
                     TASADELIAAVREVAAGGRYVEREIAAQLVVSQYGGVNPLEKLTTREVDIVRLLGEGK
                     NFVAIASALGITYKTVANACSIIKGKLGVERTSDLVRLTYQMQERQ"
     gene            complement(2128..3474)
                     /locus_tag="VGB93_05660"
     CDS             complement(2128..3474)
                     /locus_tag="VGB93_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145627.1"
                     /translation="MSLRLRLVVSIAGVLALTLIVGCALIYFDAVKKVDTEMTAALSV
                     GIRTVQNATDDAEEAANPLRQLELLIGDFDGNRHVQAFLMNPQGLPIARSMLLIPTDP
                     SPEWFDHLLARDPQIVHLDLPPPFAHYGSILLSTDSHNEIDEAWSDFQVTLVILTLFC
                     VLVLVLVYFTLGRPLALLQKMTAAFRTIGRGDYSPRMEESGAPEIADLSHCFNDMVAR
                     LANTEQQNVKLQQQLTNVQEEERADLARDLHDEIGPLLFALSVDVATLQRRSAEGKKQ
                     TGTDFDIVANAVSEIQLHVRSMLGKLRPAVLLDLGLDQAVDNLVGFWSKRRDDVHISF
                     DLDCESFGETLDSIIYRVFREGLNNALRHGKPTAVELKAKIDGESHVKVIVSDDGAGL
                     PIGGGRFGYGLVGMRERVEDAGGSLSISNRTDGKGVTVVATLPLDVVESSVSEPVT"
     gene            complement(4033..>4574)
                     /locus_tag="VGB93_05665"
     CDS             complement(4033..>4574)
                     /locus_tag="VGB93_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0145628.1"
                     /translation="SILLFVPRAAKLKAAELVVTPERVVRERLPSDPVDPNLLIYDLE
                     GELFFGAAPELDRYLEVLKKRIDAQDIKFVILRLKRARNPDVVCIERLEHFLREETAH
                     GVTILLAGVRPDTLRSLRNTGFDRWFPVDQVFPEEEEEYSATLRAVRLAYSRIPRIPE
                     SNDADSFADPLVGKLYYLV"
BASE COUNT         1015 a         1323 c         1279 g          957 t
ORIGIN      
        1 agcgtgccag attatgatgt gatcttcgtc gtcgatgcgg acgaagcctt cggcgattat
       61 ctgctttaca atagtacaga tccgcatccg gtcgtcggaa cgcaagggtt ggtcgccgtc
      121 gcctggcatc cagcgtttac ggagttttcg ggtaaggaaa tgcagcggca ctttttgcgc
      181 ctcgctcatc gcacaatgac agaacgcgac tatgaggcgt ggcttggcgt gcgtattttc
      241 ggcgaggcga tgctgcgcac gaacgcaacc gataccaaat cgttgcgcga ttaccttctt
      301 tccgacaagt tcacgatcgc ggccttcaaa ggtcagggcc tctccttccg acgatgggac
      361 ttgcagcttc ggcagcccat tcttctcgcc ggtcctcgcg ctttggtctc aatcgcgccg
      421 atcgatggat tcttgcatcc gcgtttcaac acggacacgc ttggctacga cgaaccggag
      481 acgaaatgcc atgcggttga ataaatatat tggcgcagcc cgcggcttgg cgattacgtc
      541 cggggtactg atgctcgtgg cgacgccctg ctttgccgcg aagctgttca tcaccagcga
      601 gcgggacaat accgtcacgg ttctcgacag caacaccctc accgtcaaga aggtgataaa
      661 tgtcggaacg cggccgcgag gcatcgccat cacgccagat tatcgcgaaa tctttgtctg
      721 catcggcgat gacgaccgga tggacgtgat cgacacaaaa accttggcgg tgtcgcgcag
      781 cgtccctaac gtcctcgatc cggagcttgt cgccatagat cccgtcggta aacgggttta
      841 cgccgcgaac gaggaagatt ctcaggtcac ggtcattgac cgcgcaagcg ggacaattgt
      901 cggcaccgta ggcgtcggcg cggagccgga aggcctctca atcagccccg atctctcgac
      961 ggtggtcaac acgtccgagc tgaccagcat ggcgcatttc atcaacacaa agaccttgac
     1021 gatcgaggac aatgtactcg tcgatcagcg cccgcgtgag gcgcaatata cccatgatgg
     1081 aaaacaggtt tgggtctctg ccgaagttgg tggcaccgta tctgtcatcg acgcgcaaaa
     1141 gcgcacgatt atcaaaaaga tcgaatttga tattcagggt gtgcagccgg agcttattca
     1201 gcctgtcggc atcgtctttt cgaaagacgg caagctcgct ttcgtcgctt taagccacgc
     1261 caatcgtatc gccgtcgtcg acacttcaac gctctcgcca acgtcctata tcctggtagg
     1321 tgagcggccg tggcatatgg cgctcgatcc cgacggctca aagctctatg tcgcaaacgg
     1381 aaatacgaac gacatgaccg tcattgatgt cgcgagcctg aaggcggtcc ggtccgttaa
     1441 cgtcggacat ttgccatggg gcgtcgttgc aatgccgtaa gtagcggccg agcacgatgg
     1501 ccgcaccggc ctattgccgc tcctgcatct gataggtcag gcggacgagg tcggaggtgc
     1561 gttcgacgcc gagcttgcct ttaatgatgc tgcaggcgtt cgccacggtc ttataggtga
     1621 tccccaaggc cgaagcgatc gcaacgaagt tcttcccttc gccaagcaga cgaacaatgt
     1681 cgacctcgcg cgtggtgagt ttttcaagcg gattcacacc gccgtattgc gagactacca
     1741 attgcgccgc gatctcacgc tccacgtaac gcccaccagc cgcgacttcg cgcacggcag
     1801 caatgagttc gtcggcggac gctgtcttgc tgacatatcc gcgtgcgccg gcgttgagtg
     1861 tccgcgacac atagatcggt tcggcatgca tactgaggac aacgactttt acgttccgat
     1921 tttcgatcag tagccggcgt agaagctcaa atccactcga attcggcaag tttagatcga
     1981 gcaacaaaac gtccggcgtc tctgaacggt acaacgacag cgcttcgtgc gtggaccccg
     2041 cctcgaagac agtcgtaccc ggaagcgcgg cgaacaggcg ccgcacaccc gcgcgcacca
     2101 cgacgtgatc gtcaacaatt agaagcttca tgtcaccggt tccgatacac tcgattctac
     2161 cacatcgagc ggtagagtag caacgacagt gacgccttta ccgtcggtgc ggttggagat
     2221 cgaaagggag cccccagcgt cttcaacacg ctcgcgcatg cccacaagac cgtaaccaaa
     2281 acggccgccg ccaatgggca gtcctgcgcc atcatcgctc acgataacct tgacgtgact
     2341 ttcgccgtca atcttcgcct tcagctctac agccgtaggt ttgccatgcc taagggcatt
     2401 gtttaagccc tctcggaaca ctcggtaaat tattgaatcg agcgtctcgc cgaaactttc
     2461 gcaatcgaga tcaaacgaga tatgaacgtc atcccggcgt ttgctccaga agccaacgag
     2521 attgtcaacg gcttgatcga gaccgagatc gagcagcacc gcggggcgca gcttgccgag
     2581 catagaacgg acgtggagct ggatctcgga gactgcgttc gcgactatat cgaaatcagt
     2641 cccggtctgc tttttgcctt cggcgctacg gcgttgcaag gttgcgacgt cgacgctgag
     2701 cgcaaatagc aacggcccaa tttcgtcatg caggtcacgt gcgagatccg cgcgttcttc
     2761 ctcctgcaca ttggtgagtt gctgctgcaa ttttacgttt tgctgctctg tattggcgag
     2821 acgcgccacc atatcgttga agcaatgcga gaggtccgcg atctcgggcg cgccgctctc
     2881 ctccatgcgt gggctgtaat cgccgcggcc aatggttcga aacgcagcgg tcattttttg
     2941 caagagcgcc agcgggcgcc cgagcgtgaa ataaaccagc acaagaacaa gaacacagaa
     3001 caacgtgaga atgacaagcg tgacctgaaa atcgctccag gcttcgtcga tctcgttatg
     3061 agaatcggtc gacaggagaa ttgacccata atgcgcaaac ggcggcggca gatcgagatg
     3121 aacgatttgc ggatcgcgcg ccaacaaatg gtcgaaccat tccggtgacg gatcggtcgg
     3181 aataagcagc atggaccggg cgatcggcag cccttgcgga ttcatcaaga acgcctgaac
     3241 gtgacgattg ccatcgaaat cgccgatgag cagctcaagt tggcgcagcg ggttcgcggc
     3301 ttcctccgcg tcgtcggtgg cgttttgcac cgtgcgaatc ccgacgctga gcgccgctgt
     3361 catctcggtg tcgaccttct tgaccgcatc gaaataaatc agcgcgcagc cgacaatcag
     3421 cgtgagggcc agcacgccgg ctatcgaaac aactagacgc agcctgagtg acaagaccaa
     3481 ccccgttcca gtttccgagc acgtatgtgc ggacgctcga ccgggagtat agtggcggct
     3541 gcctcgtttt ggggaggaca atgggcgccc cctgatctac aaatgttccc aaataacggc
     3601 agaaactctc ccgcggcacc ctcctacgga accgcagaaa tcaaatcgac agcagggagg
     3661 ccccgataac gaattggcaa attttttgcg ttatcttaac tttgagcgga gctttaggtc
     3721 cgcctcggcg ttgagtttcc ttcaaaacgg gagttcgcaa tgcgcgattt catcaaagct
     3781 ctttttcggc gcagcttgga cgtagccggt gcttggttgg gcgataacac cgcccgcttc
     3841 tacggcgtgc tcgtgtccac gccgcgagat caagcgtagc cgcgaccgat catcgacgga
     3901 aaacgcgtgc ggcttgataa ccagacttgg cgctcgtgag cggcttttcg tgagcctgat
     3961 gtaacacgcc tgctcaacgc gtcgatcgct ggaggcagtt tccactacgc gatctgcgat
     4021 gggttccgct gttcacacca aataataaag tttgccaaca agaggatcag cgaagctgtc
     4081 ggcatcattg ctttctggaa ttctcggaat ccggctgtac gcgagacgta cggctctcaa
     4141 cgtggccgaa tattcctctt cttcctccgg gaagacttga tccacgggga accagcggtc
     4201 gaagccggta tttcgcagag agcgcaacgt gtcgggtcga acgcccgcga gaagtattgt
     4261 tacaccatgc gcggtttcct cccgcagaaa atgctcgagc cgttcgatgc acacgacatc
     4321 cgggttgcgg gcgcgtttca agcgcaggat cacgaacttg atgtcctgcg cgtcgatgcg
     4381 cttcttgagt acctccagat aacggtcgag ttccggagcc gcaccgaaga acaattcgcc
     4441 ttcgagatca tagatgagaa gattgggatc aacagggtcc gaaggcaggc gttcacgcac
     4501 gacacgttcc ggtgtcacga ccagttccgc agctttcagt ttcgccgcgc gcggcacgaa
     4561 aagcagaatc gaca
//
LOCUS       DASWOG010000279         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_189917,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000279 DASWOG010000000
VERSION     DASWOG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189917"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1123
                     /gene="hisN"
                     /locus_tag="VGB93_05670"
     CDS             329..1123
                     /gene="hisN"
                     /locus_tag="VGB93_05670"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEY0145629.1"
                     /translation="MTAIDLTACIENLADVAGQTILPFFRTTFGAEDKSAGGVFDPVT
                     EADRAAEVAMRRLIRETFAHHGIIGEEFGNDREDAEYVWVLDPIDGTKSFISGFPTWG
                     TLIGLLHKGRPIYGAMAQPFTREYFFGDGKVTTWRGPGHDHKLIQRRLYVRACEALSR
                     ATLLTTSPLLYPPEELAKFRRVEEKTRLSRYGYDCYAFAMLAAGHVDCVIEAGVKAYD
                     IAPLIPIVEGAGGVVTTWSGGDAAAGGDVIASGDARVHEAALALLA"
     gene            complement(1120..>1698)
                     /locus_tag="VGB93_05675"
     CDS             complement(1120..>1698)
                     /locus_tag="VGB93_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0145630.1"
                     /translation="SPMIDLYLLRFKAGARLFIEGLDIIGLGGSYIPGASGRSIYLRP
                     FAQQVLTSDETRHARTVATLSAAPQLAIGGPTVSWANAALRLMRQFENPDYARRTLTP
                     ALVIATGADRIVATPAIEAFASRLKAGHFITLPQARHDILMERNVFRSQFWAAFDAFI
                     PGAEDEIAKTLRAAPPRRRRRGLLRWPLAARV"
BASE COUNT          332 a          488 c          528 g          350 t
ORIGIN      
        1 tgcaatgaag caagcggccc aagtctaggg aggaaacgcc caaggagggc atcggcgaca
       61 accgggttgt cgcaccgcaa taatatggac gtgcgtcgca caaagcgcaa gtcttattga
      121 cctatttgtg ggttgtgcgg cggtgcgacg catcgagccc gttaaagtat tattcgtgca
      181 cggatttttc ccttgaaatc gcgactttca ggcgcttctt acgcgttgcc cttgtgccac
      241 gcgggctgga tttagaggct tagttcgagt tcggcgcgtc gttcaaaatc cattgcctct
      301 atggtccgtt cgattctatc ggtctcatat gacagccatc gatctcaccg cctgtatcga
      361 aaatctcgcc gacgtcgccg gccagacgat cttgccgttt ttccgcacga cctttggcgc
      421 cgaggataaa agcgccggtg gcgttttcga tccggtcacc gaagcagacc gtgctgctga
      481 ggttgcgatg cgccgcctca tccgcgaaac gttcgcccat cacggcatca tcggcgagga
      541 attcggcaat gaccgcgagg atgcggaata tgtctgggtg ctcgatccga tcgacggcac
      601 taaaagcttc atttcgggtt ttccgacctg gggaactttg atcggtctct tgcacaaagg
      661 ccggccgatc tatggtgcaa tggcgcaacc ttttacgcgc gaatattttt ttggcgacgg
      721 caaggtcacg acctggcgcg ggccagggca cgaccacaag ctgatacaac gcaggctcta
      781 tgtccgcgcc tgcgaggcct tatcgcgcgc aacattgctc acgacttcgc cgctacttta
      841 tccgccggag gaactggcca aatttcgtcg cgtcgaggaa aagacgcggc tctcgcgtta
      901 cggctatgat tgctacgcct tcgcgatgct ggccgcaggc cacgtcgatt gcgtcatcga
      961 agccggcgtc aaggcttacg atattgcgcc actaatcccg atcgtcgaag gggcaggggg
     1021 cgtggtgacg acctggagcg gcggcgatgc ggccgctggc ggcgatgtta tagcttcggg
     1081 cgatgcgcgt gtgcatgagg ccgcgctggc attgctcgcc taaactcgtg cggccagtgg
     1141 ccaacgcaaa agaccacgtc ggcgccggcg tggcggcgca gcgcgtagcg tctttgctat
     1201 ctcatcttct gcgcctggta tgaaggcgtc gaaggccgcc cagaactggc tgcgaaaaac
     1261 gttgcgttcc atcaaaatgt catggcgcgc ctgcggcagg gtaatgaaat ggccggcctt
     1321 gagacggctc gcaaaggctt cgatcgccgg cgttgcgaca atccggtcgg cgccggtggc
     1381 aatcacgagc gccggtgtca gcgtcctgcg cgcataatcg ggattctcga actggcgcat
     1441 caatcgtagc gccgcgttcg cccagctcac ggttggaccg ccgatggcaa gttgcggggc
     1501 ggcactgagc gtcgcgacag tgcgcgcatg gcgcgtttcg tctgatgtga ggacttgctg
     1561 cgcaaagggc cgcaggtaga tcgaccggcc gctggcgccg ggaatgtaag agccgccgag
     1621 gccgatgatg tccaggcctt cgatgaagag ccgcgcgcca gccttgaagc gcaacaaata
     1681 caaatcaatc ataggcga
//
LOCUS       DASWOG010000280         8489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_6478, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000280 DASWOG010000000
VERSION     DASWOG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6478"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VGB93_05680"
     CDS             complement(<1..677)
                     /locus_tag="VGB93_05680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HEY0145631.1"
                     /translation="MPSLKDLRNRIASVKATQKITKAMQMVAAAKLRRAQLAAEAARP
                     FAERMEKILTAISGGFSGTKPPLLGGTGGDAVQLLVVCTAERGLCGAFNSSIARLARD
                     KAVELQAQGKTVKFLCVGRKGYDLLKRQFGSQIIEVIELRGVKQLGFGNADEIGRKVI
                     DLFEKGEFAVATLFFSRFRSVIQQIPTALQIIPAELPEVVGAHTHVHCETEPEPDELL
                     SVLLPRN"
     gene            complement(726..2255)
                     /gene="atpA"
                     /locus_tag="VGB93_05685"
     CDS             complement(726..2255)
                     /gene="atpA"
                     /locus_tag="VGB93_05685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0145632.1"
                     /translation="MDIRAAEISSILKKEIASFGTEAEVTEVGEVLSVGDGIARVYGL
                     DNVQAGEMVAFESGVRGMALNLETDNVGIVIFGSDREIMEGQTVKRTGAIVDVPVGKE
                     LLGRVVDALGNPIDGKGPINTSRRARVDVKAPGIIPRKSVHEPMATGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAVALDSILNQKSINQSTDESAKLYCVYVAVGQKRSTVAQFVK
                     VLEEQGALEYSIVVAATASDPAPMQFLAPFAGCAMGEYFRDNGMHALIVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDEHGAGSLTALPIIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSSAQTKSMKKVAGKIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLARGSRLTELLKQSQFSPLKIEEQVCVIYAGVN
                     GYLDKLPVEKIKPFEDGLLALLRANHADLLETIRASKDLSDGSAASLKSIIENYLKSF
                     A"
     gene            complement(2255..2818)
                     /locus_tag="VGB93_05690"
     CDS             complement(2255..2818)
                     /locus_tag="VGB93_05690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0145633.1"
                     /translation="MAQDESIVSGVGGRYALALYELAKEQGLTDQVAKDLENFAALIA
                     ESKDLKRFLSAPIYSAEEQLRALNDILTGAGISGISANFVKLVALKRRLIYIGDMIAG
                     FHKLDDQDKGRVRAEVTAAAPLKDAHIEALKTALVDFTGGKSVAVDLKIDPEIIGGLV
                     VKLGSRMIDGSLKTKLNSLRTRLKEVG"
     gene            3085..3642
                     /locus_tag="VGB93_05695"
     CDS             3085..3642
                     /locus_tag="VGB93_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0145634.1"
                     /translation="MATAVSSIALDTPAADFRLPATDGKIYSLADIAGEKASVIVFIC
                     NHCPYVIAVIDRLVADARLLLAEGIGFAAICSNDAKSHPEDSFENMRRFAERHHFPFP
                     YLHDESQDVARAYDAVCTPDFFGFDRHLKLKYRGRLDEGRTTPPPAGALRELLDAMRT
                     IAKTGHAPDHQIPSIGCSIKWKQNS"
     gene            3715..3972
                     /locus_tag="VGB93_05700"
     CDS             3715..3972
                     /locus_tag="VGB93_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145635.1"
                     /translation="MVALEVGEGYEVFLADGQKAFGAVREVSRNRQDIIVYVENAGDF
                     VIHASAVTGVHAQKVIVDRHKLEARLIEAITRAHTAEDPNI"
     gene            complement(3973..6177)
                     /locus_tag="VGB93_05705"
     CDS             complement(3973..6177)
                     /locus_tag="VGB93_05705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0145636.1"
                     /translation="MADVLVPVAIDQTYSYRVPAEFDLAEGDFVEVPLGTRQTVGAVW
                     EITDGALGSNLKALSARFDIPPLGARLRAFVDWVARWTLSPRGMVLRMCARAPFYAVE
                     EKPRIGLRRAGPEPKRMTPARARVLAAIEGNLAIVKSDLAERAACSTSVIDSLVDEGT
                     LEAVALPQEPIAASCDPDFAPPQLETAQALAAAALVDDVKAHGFSVTLLEGVTGSGKT
                     EVYFEAVSQALREGRQTLILMPEIALTAQFLDRFAGRFGARPAEWHSGISPRKRARIW
                     TGVAKGEVKVVAGARSALFLPFDDPALIVVDEEHETAYKQDDGVSYHARDMAVVRGQI
                     DKAAVILASATPSIESRVNAEQGRYKHLRLNARFGGAKLPSIEAVDLRRISPPRGKWI
                     SPPLAAAVSQALAAGEQALLFLNRRGYAPLTLCRACGHRFQCPNCTSWLVEHRFRRAL
                     VCHQCGHIERVPQHCPECDNVDTLTPCGPGVERLAEEVATLWPQAKALILSSDFPGGT
                     ARLRDELDAVANGACDIIIGTQLVAKGHNFPLLSLVGVIDADVGLSNGDPRAAERTFQ
                     VLQQVTGRAGRMGTLGKALVQTWQPEHPVIRALLSGDAERFYSEETEQRRRAGLPPFG
                     RLAALIVSAKDRASAENHARALVRTAHALPSNPKWKLAALGGLPQDDEITLLGPAEAP
                     IAVIRGQHRFRLLVRAPKSADMQGFLRALRATAPPARGSVRVAVDVDPQNFM"
     gene            complement(6366..7199)
                     /locus_tag="VGB93_05710"
     CDS             complement(6366..7199)
                     /locus_tag="VGB93_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0145637.1"
                     /translation="MRIKVGCDLHFEFSQPTPMIVVLNVHYSRASDLEQPDYVITRPS
                     VPLEGYRDSFGNWCYRFLAPAGHFEILTDTIVRDSGEWDAVAHTGAVQHPVEELPTET
                     LQFLLGSRYCETDRLADEAWRLFGNMEPGWARVQAVCDYVHDIMNFGYQYSRPTRSAY
                     EAYNERQGVCRDFAHLATTFCRCLNVPTRYCTGYITDIGLPPPYEAMDFAAWIEVYLG
                     GRWHVFDPRNNAPRIGRILVAYGRDASDVPLTHTFGPNMLSKFLVWAEEVPPLPAAAL
                     A"
     gene            complement(7336..>8489)
                     /locus_tag="VGB93_05715"
     CDS             complement(7336..>8489)
                     /locus_tag="VGB93_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0145638.1"
                     /translation="EIRAEIASFKEKALEAGGLYIIGTERHESRRIDNQLRGRAGRQG
                     DPGRSKFFLALTDDLMRIFGSDRMDSMLVRLGLKEDEAIVHPWINKALEKAQQKVEGR
                     NFDMRKNILKYDNVMNDQRKVVFEQRRDIMGQDTLEEMVTDMRHGVIDDLVGKHAPAD
                     AYPETWNIEEMAKAVEAELNITPPLADWAKEEGITDEILRERLEQASDEAYAARIEKN
                     GAGLMRYVEKQIFLQSLDHLWREHLVTLDHLRQVIGWRGLAQRDPLNEYKSEAFELFG
                     ELITQLRETTTAQLSHVEIAYEAPPLQTQSFDSLSTPANFMEALTPPAAALPPPAQAP
                     VALQPQIQRSAAPEQRVPTDPSTWGKVGRNELCPCGSGKKYKHCHGQIG"
BASE COUNT         1632 a         2606 c         2642 g         1609 t
ORIGIN      
        1 atgttgcgcg gcaggagcac cgagagaagt tcgtccggct ccggctctgt ctcacaatga
       61 acgtgcgtat gcgcgccgac gacttcgggc aattcggcgg gaatgatctg caacgcggtc
      121 ggaatctgct ggatcaccga acggaagcgc gagaagaaca acgtcgcgac ggcgaactcg
      181 cccttttcga agagatcgat gaccttgcgg ccgatctcgt cagcattgcc gaagccaagt
      241 tgcttgacgc cgcgcaattc gattacttcg atgatctgcg aaccgaattg gcgcttcaga
      301 agatcgtagc ctttgcgtcc gacgcagagg aacttgaccg tcttgccttg ggcttgcagt
      361 tccaccgcct tgtcgcgcgc caagcgtgcg atcgaggaat tgaaagcgcc gcagagccca
      421 cgttccgccg tgcagacaac gaggagttgc acggcatcgc cgccggtgcc cccgagcagg
      481 ggcggtttcg tcccgctaaa cccgccggaa atcgcggtca ggattttttc catccgctcg
      541 gcgaaaggcc gcgcagcctc ggccgccaat tgcgcccggc gcagtttcgc cgccgcgacc
      601 atctgcatgg ccttggtgat cttctgcgtc gccttgaccg aggcgatacg gttgcgcagg
      661 tccttcaacg agggcatgtc ggttccgctt tagctcattt ggctcggggc gcgcgaggac
      721 gggcctcagg cgaaagattt gaggtagttt tcgatgatcg acttgaggct cgccgccgag
      781 ccgtcggaca aatccttcga tgcgcggatg gtctcaagaa gatcggcgtg gttcgcacgc
      841 aggagcgcga gcaaaccatc ttcgaacggc ttgatcttct caaccggcag cttgtcgaga
      901 tagccgttga cgcctgcgta gatcacgcaa acctgctctt cgatcttgag cggcgagaac
      961 tgcgactgct tcagaagctc ggtgaggcgc gagccgcggg cgagaagccg ctgcgtcgtc
     1021 gcgtcgaggt cggagccgaa ctgggcgaag gccgccattt cgcgatattg cgcgagttcg
     1081 cctttgatct tgccggcgac cttcttcatc gacttcgtct gcgccgagga gccgacgcgc
     1141 gacaccgaga ggccgacgtt caccgccgga cggatgccct gatagaaaag atcggtctca
     1201 aggaatatct ggccatcagt gatcgaaatg acgttggtcg ggatataggc cgacacgtca
     1261 ttggcctgcg tctcgatgat cggaagcgcg gtgagcgacc ccgcgccatg ctcgtcgttg
     1321 agcttggcgg cgcgctccag cagacgcgaa tgcagataga agacgtcgcc cggataggct
     1381 tcgcggcccg gcggacggcg cagcagcaac gacatctgac gataggcaac ggcctgtttc
     1441 gagagatcgt cataaacgat cagcgcgtgc atcccgttgt cgcggaaata ttcgcccatg
     1501 gcgcagccgg cgaagggcgc caggaactgc atcggcgccg gatcggacgc ggtggcggcg
     1561 acgacgatcg aatattcaag cgcgccctgc tcttcgagca ccttcacgaa ttgcgcgaca
     1621 gtcgagcgct tctggccgac ggcgacatag acgcaataga gcttggcgct ctcatcggta
     1681 ctctggttga tcgacttctg attgagaatg gaatcgagcg ccaccgccgt cttgccggtc
     1741 tggcggtcgc cgatgatgag ttcgcgctgg ccgcggccga tcgggatcag cgcgtcgatg
     1801 gccttgagcc cggtcgccat cggctcatga accgatttgc ggggaatgat gccgggcgcc
     1861 ttcacgtcga ctcgggcgcg gcgcgacgta ttgatcgggc ccttgccatc gatcggattg
     1921 ccgagcgcat cgacaacgcg gccgagaagc tccttgccga ccggcacgtc aacgatggcg
     1981 ccggtgcgct tgaccgtctg gccttccatg atttcgcggt cactgccgaa aataacgata
     2041 ccgacattgt cggtctcgag attgagcgcc atgccgcgca caccggattc aaaggcgacc
     2101 atttcgccgg cctggacgtt atcgagacca taaacgcggg cgatgccgtc gccgaccgaa
     2161 agaacctcgc caacttcggt gacttcggct tcagtgccga aactggcgat ttccttcttc
     2221 aggatcgacg aaatttctgc ggcgcggatg tccatcagcc gacctctttc aagcgtgtgc
     2281 ggagtgaatt gagtttggtt ttgagcgatc cgtcgatcat gcgcgaaccg agcttgacga
     2341 cgagaccgcc gatgatctcg ggatcaatct tcagatcgac ggcgacggat ttgccgccgg
     2401 tgaaatcgac gagcgccgtc ttgagggcct cgatatgggc gtccttcaac ggcgcagcag
     2461 ccgtcacttc ggcgcggacg cggcctttgt cctgatcgtc gagcttgtgg aagccggcga
     2521 tcatgtcgcc gatatagatc agccggcgct tgagcgcgac gagtttgacg aaattggcgg
     2581 agatgccgga aatgccagcg cccgtgagaa tgtcattcag cgcccgcaac tgctcttcag
     2641 ccgaatagat cggcgccgag aggaacctct ttaggtcctt gctttcggcg atcagcgcgg
     2701 cgaaattttc aagatccttg gcgacctgat cggtcagacc ctgctccttg gcgagttcat
     2761 agagcgccag agcgtaacgg ccgccgaccc ccgatacaat gctctcgtct tgcgccaaga
     2821 caataaccct taaatccgtt ggcgaaacgc cccggcttcc aaagctcgcg cggcaattcg
     2881 cggcgatggc gagcgggcgt catagaagcc cggtcaggca aggaaaaacg ccaacccctc
     2941 ccaaggcgaa gggtccctaa catatggcat ttttgtgcgc aacccgtcct ggagtgcata
     3001 gtggttcggc aatttctctg tgcgctgtga ggttggcgcg ggctgacctt ttgcctatat
     3061 tttggcctca cctcggaggc gatcatggcc actgcagtca gctcgattgc tctcgacacg
     3121 ccggcggcgg actttcggct tcccgcgaca gacggcaaga tttattcgct tgccgatatc
     3181 gcaggcgaaa aggcgagcgt catcgtcttc atctgcaatc attgccctta tgtgatcgcc
     3241 gtgatcgacc gtctcgttgc cgacgcgcgg cttctgctag ctgagggcat tggcttcgcc
     3301 gcgatctgtt ctaacgacgc gaaaagccat cccgaagatt cattcgagaa tatgcgccgt
     3361 ttcgccgaga gacatcactt cccttttcct tatctccatg atgagagtca ggatgtggcc
     3421 cgcgcgtatg atgccgtctg cacgccggat ttcttcggct tcgaccggca tctgaaattg
     3481 aagtaccgtg ggcgactgga cgagggtcgc acgactccgc cgccggccgg tgcgctccgc
     3541 gaactgctcg acgcgatgcg gacgatcgcc aagaccggcc atgcgccgga ccaccagatt
     3601 ccttcgatcg gctgttcgat caaatggaag cagaattcct aggattttcg gcgcgtttct
     3661 tcacgcgaac cggcatccac ttcgctcgaa aacgctttaa caggagagag aacaatggtc
     3721 gcgcttgaag ttggcgaagg atacgaagtg tttctggccg acggccagaa ggcgttcggc
     3781 gccgtgcgcg aagtctcgcg caaccgccag gacattatcg tctatgtcga gaatgccggc
     3841 gatttcgtca ttcacgcttc cgccgtcaca ggcgttcacg ctcagaaggt gatcgtcgat
     3901 cgccataagc ttgaagcgcg tctcatcgag gcgatcacgc gcgcacacac ggcggaagat
     3961 ccgaatatct agctacataa aattctgcgg atcgacatcg acggcaacgc gcacgctgcc
     4021 gcgcgctggt ggcgccgttg cccgcagtgc gcgcagaaag ccctgcatat cggccgactt
     4081 cggcgcccgc accaaaaggc ggaaacgatg ctggccccgg atgacagcga tcggcgcctc
     4141 ggccggccca agcaaggtga tttcgtcgtc ctgtggcagg ccgccgagcg cggcgagctt
     4201 ccatttcgga ttggacggca gcgcatgtgc cgtacgcacc aaagcgcggg cgtgattttc
     4261 cgccgacgcg cggtccttgg ccgagacgat cagcgccgcg agccgcccga agggcggcag
     4321 gccggcgcgg cggcgctgct ccgtctcctc gctgtaaaag cgctcggcat cgcctgagag
     4381 caacgcgcgg atcacgggat gctccggctg ccaggtctgc accagagcct tgcccagcgt
     4441 ccccatgcgc ccggcgcggc ccgtcacctg ttgcaggacc tggaacgtcc gctccgccgc
     4501 gcgcggatcg ccgttcgaga gcccgacatc ggcatcgatg acgccgacga gcgagagaag
     4561 cggaaaatta tgtcccttgg cgacaagctg cgtgccgatg atgatatcgc aagcgccgtt
     4621 ggcaacggca tcgagttcgt cgcgcagccg cgcagtgccc cccgggaaat ccgacgagag
     4681 gatcaacgcc ttcgcctgcg gccaaagcgt cgcgacttct tctgccagtc gctcgacgcc
     4741 ggggccgcac ggcgtcagcg tatcgacgtt gtcgcattcc ggacaatgct gcggcacgcg
     4801 ctcgatatgg ccgcattgat ggcagacgag cgctcggcgg aagcgatgtt caacgagcca
     4861 ggatgtgcaa ttcggacatt ggaaacgatg cccgcaggcg cggcagagcg tcagcggcgc
     4921 atagccgcgt cgattgagaa aaagcaaagc ctgttcgccg gcggcgagcg cctggctcac
     4981 tgcggcagcg agcggcggcg aaatccattt gccgcgcggt ggtgatatgc gacgcagatc
     5041 gacggcttcg atcgacggta atttggcacc gccgaagcgc gcattaagcc tgagatgttt
     5101 gtagcggccc tgctccgcat tgacgcgcga ttcaatcgac ggtgtcgccg aggcgaggat
     5161 caccgccgcc ttgtcgatct gcccccgcac cacggccatg tcgcgcgcat gataggagac
     5221 gccatcatcc tgcttgtagg ccgtttcatg ctcttcatcg acgacgatga gcgcgggatc
     5281 gtcgaaggga aggaacaacg ccgaccgcgc gccggcgacg actttcacct cgcctttggc
     5341 gacacctgtc cagatgcgag cgcgcttgcg cggactgatg cccgaatgcc attcggcggg
     5401 acgcgcccca aagcgccccg caaaacggtc gaggaattgc gccgtcagcg cgatctccgg
     5461 cattaggatc agcgtctgac gcccctcgcg cagcgcctgc gagacggcct cgaaataaac
     5521 ctcggttttg ccagagccgg tgacgccttc gaggagcgta acggagaagc catgcgcttt
     5581 gacgtcatcg acgagtgccg cggcggctag ggcttgcgcc gtctcaagct gcggcggcgc
     5641 gaaatccgga tcgcacgagg ctgcaatcgg ctcctgcggc agtgcgacgg cttccagcgt
     5701 gccttcatcg acgagtgaat cgatcacgct cgtcgaacac gcggcgcgtt cggcgagatc
     5761 acttttgacg atggcgagat tgccctcgat tgcagcaagg acacgcgccc gcgcgggtgt
     5821 catacgcttg ggctccggcc cggcacggcg cagaccgatg cgcggctttt cctcgacggc
     5881 ataaaacggc gcacgggcgc acatccgcaa caccatgccg cgcggactta acgtccagcg
     5941 cgcgacccaa tcgacgaacg cgcggagccg ggcgccgagc ggtggaatat caaaccgcgc
     6001 agagagggct ttcaggttgc tgccaagcgc gccgtccgtg atctcccaga ctgcgccgac
     6061 cgtctggcgc gtaccgagcg gcacttcgac gaaatcgccc tcggcaaggt cgaactcggc
     6121 cggaacacgg taagaatagg tctgatcgat cgcgacgggg acgagaacat cggccacgcg
     6181 cggcccagga gcgggatgcg aaaaagtgga ccccggtttt tcgcgtaaat cccgctccga
     6241 tctaacccct ttacgactcg gcgtgcgctc gctcatgcgc gagccttaaa acatgttgcg
     6301 gccaagttgc acgacttttt cggtccgttg gtccgacggc agaaccacgc gcggcgactt
     6361 caaagtcagg ccagtgcagc ggccggcagc ggcggcactt cttcggccca gaccaaaaat
     6421 ttcgacagca tgttgggtcc gaaagtgtgc gtcagcggca catcggaggc gtcgcggccg
     6481 taggcgacga ggatgcggcc gatgcgcggc gcattgttgc gcggatcgaa gacatgccaa
     6541 cggccgccga gataaacttc gatccaggcg gcaaaatcca ttgcctcata cggcggcggc
     6601 aggccgatat cggtgatgta gccggtgcaa taacgcgtcg gaacgttgag gcagcggcaa
     6661 aacgttgtgg cgagatgtgc aaagtcgcgg cacacgcctt ggcgctcgtt ataggcctcg
     6721 tacgccgacc gcgtcgggcg cgaatattgg tagccgaaat tcatgatgtc gtggacgtaa
     6781 tcgcaaaccg cctgcacgcg cgcccagcca ggctccatat tgccgaagag gcgccaggcc
     6841 tcgtccgcca gccgatcggt ctcgcaatag cggctgccga ggaggaattg cagcgtctcg
     6901 gtgggaagct cctcaaccgg gtgctgaacg gcgcccgtgt gtgcgacggc gtcccattcg
     6961 ccggaatcgc ggacgattgt atcggtcaaa atctcgaaat ggccggccgg cgcgaggaag
     7021 cgatagcacc aattgccgaa actatcgcga tagccttcga gcggcacgct cgggcgcgtg
     7081 atgacataat ccggctgttc gagatcggat gcgcgcgaat aatgcacatt gagcacgacg
     7141 atcatcggcg tcggctgcga aaactcgaaa tggaggtcgc acccgacctt gatgcggatc
     7201 ataggacttt cctgcgcgag agagccgggg ccggaaagac acgaagcaag tcccacgccg
     7261 gtctgcgaca acccttggct tggctttaag ttccgcgcgg aatcccttcg acgaaggtcg
     7321 aagccgcgtg ccgccttagc cgatctggcc gtggcagtgc ttgtatttct tgcccgaccc
     7381 gcacgggcaa agttcgttgc ggccgacctt gccccaggtc gaagggtcag tcggcacccg
     7441 ctgctccggc gccgcgcttc gctggatctg cggttgcagc gcgaccggcg cttgcgccgg
     7501 cggcggcaac gcggccgcag ggggcgtcag cgcctccatg aaattcgccg gtgtcgatag
     7561 cgaatcgaag ctctgcgtct gcagcggtgg cgcctcgtaa gcaatctcga catggctcaa
     7621 ctgcgccgtc gtcgtctcgc gaagctgcgt gatcaattcg ccaaaaagct cgaaagcctc
     7681 cgacttatat tcgttcagcg gatcgcgctg cgccaagccg cgccagccaa tcacctgacg
     7741 cagatggtcg agcgtgacga gatgttcacg ccagagatga tcaagcgatt gcagaaagat
     7801 ctgcttctcg acatagcgca tgaggccggc gccattcttc tcgatgcgcg ccgcataagc
     7861 ctcgtcggac gcctgttcca ggcgctcgcg caaaatttcg tcggtaatgc cttcttcctt
     7921 cgcccaatcg gcaagcggcg gagtgatgtt gagttcggcc tcgacggcct tcgccatttc
     7981 ctcgatgttc caggtctcgg gataggcgtc ggccggcgca tgtttgccga cgagatcgtc
     8041 aatgacaccg tggcgcatgt cggtgaccat ttcttcgagc gtgtcctgac ccatgatgtc
     8101 gcggcgctgc tcgaagacca ccttgcgctg gtcgttcatg acgttatcgt acttgagaat
     8161 gttcttgcgc atgtcgaagt tgcggccttc gactttctgc tgcgcttttt cgagcgcctt
     8221 gttgatccag ggatggacga tcgcctcgtc ttccttgagg ccaagccgca ccagcatcga
     8281 atccatgcga tccgaaccga agatgcgcat gagatcatca gtcagcgcta ggaagaattt
     8341 cgagcggccc gggtcgccct gacggccggc gcggccgcgc aactggttgt cgatgcggcg
     8401 gctttcgtgc cgttcggtgc cgatgatgta gagaccgccg gcttcgagcg ccttttcctt
     8461 gaaactcgcg atttcggcgc gaatctcac
//
LOCUS       DASWOG010000281         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_336029,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000281 DASWOG010000000
VERSION     DASWOG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336029"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..837)
                     /locus_tag="VGB93_05720"
     CDS             complement(139..837)
                     /locus_tag="VGB93_05720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY0145639.1"
                     /translation="MSVINSIRKQIPPIHPEGYIFIAIFAVITLVAGWIFDPLFWIGL
                     LLTIWCALFFRDPLRVTPQASDLVISPADGKVSAVGLAAPPPELGLGADPLLRISIFM
                     SVFNVHVNRAPMAGRVAKIAYRPGVFLNADLDKASEDNERNGLVIDTAAGRIGVVQIA
                     GLIARRIVCFVEQGDSVDAGERFGLIRFGSRVDVYLPHGTRALVAEGATAIGGETILA
                     DFHADEPARLFKLA"
     gene            complement(850..2823)
                     /locus_tag="VGB93_05725"
     CDS             complement(850..2823)
                     /locus_tag="VGB93_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0145640.1"
                     /translation="MLQTNPISSSALVKHSWSANLAASVAGISEVWRKLWPYVWPSGR
                     RDLQMRMYATFVLLIVGKLVTVAVPYAFKWATDELSGHHLTHHILGWVVGPLALTLLY
                     GLMRIIMAALTQARDGIFADVAMHAVRRLANDVFIHLHLLSLRFHLQRRIGGLTRILE
                     RGRDAIETIVRTVVLVAAPTAVEFLLILAVVFFQFDWRYTAAVAVMIAAYTAFTLVAT
                     NWRIGIRRTMNESDVDANSKALDSLLNYETVKYFGAEGREGARYDKSMARYEENSVKS
                     YTSLTFLNFGQALIFSLGLTVVMAMSIMGVRAGHNSVGDFVLINAMMIQLYQPLNFLG
                     SIYRDIRQATIDLEMMFGILSEYPDIADKPGAKPLVVSGGDVRFDNVRFNYDPEREIL
                     KGVSFEVPAGKTVAIVGPSGAGKSTLSRLIFRFYEPSSGAITIDGQNIDNVTQKSLRA
                     VIGMVPQDTVLFNDTIAYNIRYGRADASDADMREAARHAQIDKFIESLPSGYQAQVGE
                     RGLKLSGGEKQRVAIARTILKAPPILVLDEATSALDSFTEGEIQDALDRVSRGRTTLV
                     IAHRLSTVVNADQILVLDQGRIVERGSHTVLLEKNGVYAAMWNRQNEVRRAEEVLRRA
                     AAEEQGATHPAEDDTAELVGRSYAEDDADIALG"
     gene            complement(2959..4161)
                     /locus_tag="VGB93_05730"
     CDS             complement(2959..4161)
                     /locus_tag="VGB93_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0145641.1"
                     /translation="MRPPQIAALGLGAAVVAAGAGLVWHEYRANAPAPRPLSFAAVEP
                     AKPEPQVPATPPPQQTAKVEPAVPPEVPAPPPAADLAPTLPQFDTVRVEPSGDAVVAG
                     HGAPGAQVALLANGKPIANEVSDGAGNFVIIPTQLPPGNYNLTLRSERPGKPAEVSVQ
                     VVTVSVPARGQKNLVVAVAEPGKPTRILADTSAPAKPIVPPAPGAMQAPSVNFKTAEV
                     DKDGFYATGSATPAGVHLRIYLNGTHIADLAAAADDHWSLTVKRGLSPGHYSLRADAL
                     DGAQKVSARAEVGFDVPLLTASAAPTPPRSAVTQSDAEPTPAMSAPGSMQQRAEAPPA
                     ASTLNDAVVPTLGTATVIHGDSLWRISRKILGHGVRYTLIYEANASQIRDPNLIYPGQ
                     VFVVPPQP"
     gene            4347..>4635
                     /locus_tag="VGB93_05735"
     CDS             4347..>4635
                     /locus_tag="VGB93_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145642.1"
                     /translation="MTATIDFQDSPLATPGNGLIRNVCVYCGSGQGTTPIHVEAAAAF
                     GRVLAGAGIGLVYGGGNTGLMGAVARAVLAEGGHVTGIIPQFLSDREVALEA"
BASE COUNT          898 a         1441 c         1454 g          842 t
ORIGIN      
        1 cccgcgcacg agtccgagcc ggcgctcgcc ccgtcccggc tcgcgttcat gcacagaaaa
       61 tcgtcgtggc aaaggatcgc tcatcggcgc cttcataaag caaattgtgg cacgacggaa
      121 acgggcctga tctcggccct aggccagctt gaacaggcgc gccggctcat cggcgtggaa
      181 atcggccagg atggtctcgc cgccgattgc ggtcgcgcct tccgccacca gcgcccgtgt
      241 cccatgcggt aggtagacat cgacccgcga accaaagcga atcaggccga agcgctcgcc
      301 cgcgtcgacg ctgtcgccct gttcgacgaa gcagacgatt cgccgcgcga tgagtccggc
      361 gatctgaacg acgccgatcc gccccgcggc ggtatcgatc acaagtccgt tgcgctcgtt
      421 gtcttcgctg gccttatcga gatcggcgtt cagaaagaca cccggccggt aggcgatctt
      481 ggcgacgcgg cccgccatcg gcgcgcgatt cacatgcaca ttgaagaccg acataaaaat
      541 cgagatgcgc agcaaggggt cggcaccgag acccaattcc ggtggcggcg cggcaagacc
      601 cacggcggaa accttaccgt ccgccggtga aatgacgaga tcactcgcct gaggcgtcac
      661 ccgcagcgga tcgcggaaga aaagcgcgca ccagatcgtc agcagcaagc cgatccagaa
      721 caagggatcg aagatccagc cggcgacaag cgtaatcacc gcgaaaatcg cgatgaaaat
      781 atagccttcc ggatggatcg gcggaatctg cttgcggatc gagttgatga cgctcatgcc
      841 tgcaaggctt tatcccaatg cgatgtcggc gtcatcttcg gcgtaagagc gcccaacaag
      901 ctcggctgta tcatcctctg ccgggtgcgt ggcaccctgc tcttccgccg cggcccggcg
      961 caagacttcc tcggcgcgcc gaacctcgtt ctgccggttc cacatcgcgg catagacgcc
     1021 atttttttcc agcaacactg tgtgcgatcc gcgctcgacg atccgcccct gatcgagaac
     1081 caggatttga tcggcattga cgacggtaga gagtcggtgc gcgatgacaa gcgtcgtgcg
     1141 cccgcgcgag acgcgatcga gcgcatcctg gatttcgccc tcggtgaagc tatcgagcgc
     1201 cgaggtcgcc tcatcgagca cgaggatcgg cggcgctttc agaatcgtcc gggcaatggc
     1261 gacgcgttgt ttctcgccgc ccgatagttt cagaccacgc tcaccgacct gcgcctgata
     1321 gccgctcggc aggctctcga tgaacttatc gatctgggca tggcgcgccg cctcgcgcat
     1381 gtcggcatca ctcgcgtcgg cgcgcccgta gcgaatgttg taggcgatcg tatcgttgaa
     1441 gagcacggtg tcctgcggca ccatgccgat cacggcgcgc agtgatttct gcgtcacgtt
     1501 gtctatattc tggccatcga tcgtgatcgc gcccgatgag ggctcataga aacggaagat
     1561 cagccgcgag agagtcgatt tgccggcgcc ggacggaccg acaatggcaa cggtcttgcc
     1621 cgctggaacc tcgaagctca cgcctttcaa aatctcacgc tcgggatcgt aattgaagcg
     1681 cacattgtca aagcggacat cgccgccgga gacaacaagc ggcttggcgc cgggcttgtc
     1741 ggcgatatcg ggatattcgc tgagaatgcc gaacatcatc tctaggtcga tcgtcgcctg
     1801 gcgaatgtcg cgatagatcg agccgagaaa gttcaacggc tgataaagct ggatcatcat
     1861 cgcgttgatc agcacgaaat cgccgacgct gttgtgtccg gcgcgaaccc ccatgatgct
     1921 catggccatg acgacggtga gccccagaga aaagatcagc gcctgaccga aattcagaaa
     1981 cgtcagtgat gtataagatt tgacgctgtt ctcctcgtaa cgcgccatcg atttgtcgta
     2041 gcgtgccccc tcgcggccct cggcgccgaa atatttgacc gtctcgtaat tcagcagtga
     2101 atcgagagcc ttactgttcg catcgacatc gctctcgttc atcgtccggc gaatgccgat
     2161 tcgccaattg gtggcaacga gtgtgaaggc ggtataggcc gcgatcataa ccgcgacggc
     2221 cgcggtgtag cgccagtcga actgaaagaa gacgacggcg agaatcagca agaattcgac
     2281 ggccgttggc gcggcaacga gcacgaccgt gcgcacgatc gtttcgatag cgtcgcggcc
     2341 gcgttcgagg atgcgcgtca ggccgccgat gcgccgctgc aaatgaaagc gcagcgacag
     2401 aagatgcaaa tggatgaaga cgtcattggc gaggcgccgc actgcgtgca tcgctacatc
     2461 agcaaagatc ccgtcgcgcg cctgcgtgag ggcggccatg atgatgcgca tcaagccgta
     2521 aagcagcgtc aaagccagcg gcccaaccac ccagccgaga atgtgatgcg tcaggtgatg
     2581 gccggagagt tcgtcggtcg cccatttgaa cgcatagggg acggcaaccg tgacgagctt
     2641 gcccacgatg agcagcacga aggtcgcgta catccgcatt tgcagatcgc ggcggccact
     2701 cggccagaca tagggccaga gtttcctcca aacctctgag atgccagcca cgctggccgc
     2761 gagatttgcg gaccacgaat gtttgacgag cgcggaggac gatatcggat tggtttgcag
     2821 catatttctc tttgaacgac agatcggcgc tggtcatcgc aagcgctcgg cagcggcgct
     2881 ggctttcata taggctcacc cgtgcaaatc cctagtcggc cgcccagatg ccgcggacgc
     2941 ccttgagcta aagccgcttt agggctgcgg cggcaccacg aaaacctgac cgggatagat
     3001 cagattcgga tcgcgaattt gcgacgcatt ggcctcatag atcaacgtat agcggacgcc
     3061 atggccgagg attttacggc tgatccgcca gaggctgtcg ccgtgaatga ccgtggccgt
     3121 tccgagggtc ggcacaacgg catcgttcaa ggttgaagcg gccggcggcg cctcagcccg
     3181 ctgctgcatc gacccgggcg cggacatggc gggcgtcggt tcggcatcgg attgcgtcac
     3241 cgcgctacgc ggtggggtcg gtgccgccga ggccgtgaga agcggcacgt cgaagccgac
     3301 ctccgcgcgg gcgctgacct tttgcgcgcc gtccaaagcg tcggcgcgca aggaataatg
     3361 gcccggcgag agaccgcgct tcacggtcag cgaccaatgg tcgtcggccg cggcggcaag
     3421 atcagcaatg tgggtgccat tgagatagat ccgcaaatgc accccggccg gcgtcgccga
     3481 gcccgtggca tagaatccat ccttatcgac ctcggccgtt ttgaaattga ctgagggcgc
     3541 ctgcatcgcg ccaggcgcag gcggcacaat cggcttcgcc ggtgcggacg tgtcggcgag
     3601 gattcgcgtc ggcttgcccg gctcggccac ggcgacgacc aggtttttct ggccgcgcgc
     3661 cggcacagat acggtcacga cctgaacgga cacttcggca ggcttgccgg gccgctcgct
     3721 ccgcaatgtc agattataat tccccggcgg cagttgcgtc gggatgatga cgaaattgcc
     3781 agcgccgtcc gagacttcgt tagcgatcgg cttgccgttg gcgagcagcg cgacctgcgc
     3841 gccgggcgcg ccatgacccg cgaccactgc gtcgcctgag ggttcgaccc ggaccgtatc
     3901 aaactgtggc agggtcggcg cgaggtccgc cgcgggcggc ggcgccggca cttccggcgg
     3961 cactgccggc tcgacttttg cggtttgctg cggcggcggt gtcgcgggaa cctgcggctc
     4021 gggcttcgcg ggctcgacgg ccgcaaaaga aagcgggcgc ggcgcgggcg cattcgcccg
     4081 atattcgtgc caaacgagcc cagcaccggc ggcgaccacg gcggcaccga gcccaagggc
     4141 tgcaatttgt ggtggcctca tcggcgcgtc ctcctcgcgg acttacttat ccgtcgtttg
     4201 gttctcagtt tcatttaact gcattatttg aatagtgaag cggaatcata gaggtcgcga
     4261 ggcgccgtcc cccgtgtctt gcggcgcgac atcacttttt cccggaattt cttgaccctt
     4321 ttgggcgcgg gcgcgacaag cgcgccatga cagcaacaat cgattttcag gattcgcccc
     4381 tcgccacccc aggcaacggc ctcatccgca atgtctgcgt ttattgcggt tcggggcaag
     4441 gaacaacgcc tattcacgtc gaggctgcgg ctgccttcgg gcgcgtcctc gctggcgcgg
     4501 gcattggcct cgtgtacggc ggcggcaaca ccgggctgat gggcgcggtc gcgcgcgccg
     4561 ttctcgccga aggcggccat gtcaccggca tcatcccgca attcctgtcg gatcgtgagg
     4621 ttgcgctcga agccg
//
LOCUS       DASWOG010000282         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_24631,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000282 DASWOG010000000
VERSION     DASWOG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24631"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..>1585)
                     /gene="rpoN"
                     /locus_tag="VGB93_05740"
     CDS             complement(290..>1585)
                     /gene="rpoN"
                     /locus_tag="VGB93_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0145643.1"
                     /translation="TDRGPLEASLGTELSNTFDDDRPPTPAEHAPSGEGLLLSSNSWS
                     GAPGPASDSSDATNLEAYVAAQTSLREHLSAQLVLATKDPVERVIGQAIIDSIDDSGY
                     LMDPLAEVALRLGAPLERVDKVLRVIQTLEPAGVGARNLAECLSIQLREKDRFDPAMQ
                     MLVSRLDLVAKRDYAGLRKLCGVSAEDVTDMIAEIKRLDPKPGRVFGGGVVQPVVADV
                     IIRALPDGSWHVELNSEVLPRLLVNQVYATRVARAKANDVDRSFLSNCLQTANWLTKS
                     LEQRARTILKVASEIVRQQDAFFAHGVEHLRPLNLKAIADAIGMHESTVSRVTSNKYV
                     STPRGLFELKYFFTASIASHSGGDAHSAEAVRFRIKQMIEHESENDILSDDAIVERLK
                     KSNVEIARRTVAKYRESLRIPSSVERRRMRSLHYTSSVT"
BASE COUNT          326 a          446 c          491 g          322 t
ORIGIN      
        1 cgacaaggtc gagtcgcgca tcgacataag tgcgaagcga agccccgata tcgatatttt
       61 ttccagagac gcgcaatgtc attggtttac ctccaatcca ctgtcgcccc aacacaatcc
      121 gccgtttacg gcagttgctt cagggttagg agcctcgcgc ggttaagtca atgggcgccg
      181 ccggcgcatg gcgcaaaggg ggaaaactcc gcctgaacgc aacggcaacg ctttacgcca
      241 agtgcgctat ttgcgaagcg tttccagctt ttgtggaaat gttcaaagtt cacgtgacgc
      301 tcgaagtgta atgcaggctg cgcatacgcc gacgctcgac cgatgacgga atccgcaaac
      361 tttcacgata tttcgccact gtgcggcgcg caatttcgac attcgatttt ttcaatcgtt
      421 ctacgatcgc atcatccgac agaatatcat tttcggattc atgctcgatc atctgtttga
      481 tgcgaaaccg caccgcttcc gccgagtgag cgtcgccgcc gctgtgcgat gcgatagagg
      541 cggtgaagaa atatttcagt tcaaagaggc cgcgcggcgt cgaaacatat ttgttcgacg
      601 tgacgcgcga aacggttgat tcatgcatgc cgatggcgtc ggcgatcgcc ttcagattga
      661 gcggtcgcag gtgttcgacg ccatgcgcga agaaagcgtc ctgctgacgg acgatttcgc
      721 tcgcgacttt cagaatcgtg cgggcgcgct gttcgaggct cttcgtcaac caattggcgg
      781 tctgcagaca gttggacaga aaagagcgat cgacatcatt ggctttggcg cgcgcgacgc
      841 gcgttgcata aacctgattg acgagtaggc gcggcaaaac ctcggaattc aattcgacat
      901 gccaggagcc gtcgggcagg gcgcggatga tgacgtccgc gacgaccggt tgcacgaccc
      961 cgccgccgaa gacacggcca ggtttcgggt cgaggcgctt gatctcggcg atcatgtcgg
     1021 tcacgtcttc cgcgctgacg ccgcaaagct tgcgcagacc cgcgtagtcg cgcttggcga
     1081 caaggtcgag acggctgacg agcatctgca tcgccggatc gaaacggtct ttctcacgta
     1141 attggatcga caggcattcg gcgaggttgc gggcgccgac gccggccggt tccaatgtct
     1201 gaatgactcg tagaacttta tcgacacgct caagcggcgc gccgagtcgc aacgcgacct
     1261 cggccagagg gtccatcaga tagccggaat cgtcgatgga atcgataatc gcctggccga
     1321 tcacccgttc gaccggatct ttggtggcga gcaccaattg cgcggaaaga tgctcccgca
     1381 agctggtttg ggccgcgaca taggcctcga gattggtcgc gtcagaactg tcggacgcgg
     1441 ggccaggggc gccggaccag gagttggacg acagcagcag cccctcgccg gacggggcat
     1501 gttcggcggg cgtcgggggg cggtcgtcat cgaatgtgtt ggacagttcg gtgccgagac
     1561 tggcttccag cgggccgcgg tcggt
//
LOCUS       DASWOG010000283         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_354408,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000283 DASWOG010000000
VERSION     DASWOG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354408"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGB93_05745"
     CDS             <1..457
                     /locus_tag="VGB93_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0145644.1"
                     /translation="NITRFTSTDYVSALGNGDICLAVGWSGDASQARRGAEEADNGTE
                     LGDATPKEGSVLGLDNLAIPRNAPHLAAAYVFLDYLMRPEIAARNSEATGFASGILAA
                     KALLPHDFANNKAIYPDDDTMSRLYALTNPDPMVERYITRVWSLIKAAK"
     gene            580..>1503
                     /gene="zapE"
                     /locus_tag="VGB93_05750"
     CDS             580..>1503
                     /gene="zapE"
                     /locus_tag="VGB93_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY0145645.1"
                     /translation="MPNILLERYSERVKRGEFERDPAQEQVLEQLQALSTQLAFYRPT
                     RKSAALGWLVGARQKAAPLFGIYIWGSVGRGKTALMDMFFEETELSHKRRTHFHAFMA
                     DVHARIFAVRQQIKDGRVKGDDPIAPVAEALARETWLLCLDEFTVTDIADAMILARLF
                     KALLQAGVIVVATSNVPPEDLYKDGLNRALFLPFLDILDAHMSVMRLDSRTDFRLEKL
                     KGEPVYHVPADYKAHAALTRAFAALTGVEKASGKNLDFLGRSLRVPQAKNRVARFHFA
                     DLCVAALGARDFLAIAENFHTIIIDDIPVLDN"
BASE COUNT          313 a          469 c          431 g          290 t
ORIGIN      
        1 caacatcacc cgcttcacat cgaccgatta tgtgagcgca ctgggcaacg gcgacatttg
       61 cctcgcagtc ggctggtccg gcgatgcgtc gcaagcgcgc cgcggggccg aggaagcgga
      121 caacggcacc gaacttggcg acgcgacgcc caaggaaggc agcgtgctgg gcctcgacaa
      181 tctggcgatc cccaggaatg cgccgcatct tgccgccgcc tatgtcttcc tcgattatct
      241 catgcggccc gaaattgctg cgcgcaacag cgaggcaacg ggctttgcca gcggcattct
      301 tgcggcgaaa gccctgctcc cgcatgactt cgccaacaac aaagcgatct atcccgacga
      361 cgatacgatg tcccggctct acgctctcac caacccggac ccgatggtcg aacgctacat
      421 cacgcgtgtc tggtcgctga tcaaggcagc gaaataagca cgtcaagaaa tacgggtggt
      481 ttggccgcgt cgcaaaatca cacttcaccg cagccgcata gtttgcacaa tggctgcact
      541 gaacccaaaa tcgacgcatg aacatcgtcc ggagtccgcg tgccgaacat tctccttgag
      601 cgttattcgg agcgcgtgaa acgcggcgaa ttcgagcgtg atccggcgca agagcaagtg
      661 ctggaacaat tgcaggccct ttcgacgcaa ctcgcctttt accggccgac gcgcaaaagc
      721 gccgcgctcg gctggcttgt tggcgcgcgt caaaaagcag cgccgctttt cggcatttat
      781 atctggggct cggtcgggcg cggcaaaacc gcgctcatgg acatgttttt cgaggagacg
      841 gaactcagcc ataaacggcg gacgcacttc cacgccttca tggccgacgt acatgctcgc
      901 atctttgcgg tccgccagca gatcaaggac ggccgcgtga aaggcgacga tccgatcgcg
      961 ccggttgcgg aagcgctggc acgcgagaca tggctgctgt gcctcgatga attcaccgta
     1021 acagatatcg ccgatgcgat gatcctggcg cggctgttca aggcgctttt gcaagccggc
     1081 gtcatcgttg tcgcaacgtc gaatgtcccg ccggaggacc tttataaaga cggcttgaac
     1141 cgcgcgttgt ttttgccgtt cctcgacata ttggatgcgc atatgtcggt gatgcggctt
     1201 gattcacgca ccgacttccg gcttgaaaag ctcaagggcg aaccggtcta ccacgtcccc
     1261 gccgactaca aggcacacgc ggcattgacg cgagcgttcg cggctctcac cggcgtcgaa
     1321 aaggcgagcg gtaaaaatct tgactttctg gggcgcagtc tgcgcgtgcc gcaggcgaag
     1381 aaccgcgtcg cacgattcca cttcgccgac ctctgcgtcg ccgcgctcgg ggcgcgcgat
     1441 ttcctcgcca tcgccgaaaa ttttcacacg attatcatcg atgacattcc ggtcctggat
     1501 aat
//
LOCUS       DASWOG010000284         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_190006,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000284 DASWOG010000000
VERSION     DASWOG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190006"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /locus_tag="VGB93_05755"
     CDS             <1..1724
                     /locus_tag="VGB93_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEY0145646.1"
                     /translation="VESGTLEVGDAADLGATLTSTTPIDVFGTLAGHGTIIGGATIEN
                     GGTLAPGGSIGTLTIDGNLAFDPGGFYSVAIAPGQNSLTQVDGSVMLGGARVVASLSG
                     FALGRHTGSTETILTTTNPLGARNQFNPAVAFRFEGTSAEQFDPLGAVVLAETSNSVL
                     LTLPDFAVTLALPSNAPTNAQHVAGAINQFIVGGGTLPVGLQGLANLTGVALNNAANS
                     LAGQTQGSFVPVGFDAGTMFLNLLLNPFVDGREAAGEFDSPSSGSRLAYAADEAKPAA
                     YQALASALSPAAIGFAPHISLWASAYGGEGTIGGNVATGASSATSQIYGFAAGADDHI
                     ASDTMFGLAIGGGGTNWQLNGLGGGSSGLFQMGAYGTRLFGPAYVSGALAYSLQDVRN
                     SRDAEVGTSDPLHGDFVANVLSGRIESGYHLPFGAITLTPYGAVQSQTMFLPSYGEVG
                     AAGASSFALTYDGRTTTDTRTEVGAWFDTDLPSLFSGNVKLYGRAAFAHDFENEGSTT
                     ALFQSLPGSGSFIINSAKPDDNSALVTAGLEYKLGAGWSVLAKFDGEFSGNSTIVGGT
                     GVLRKVW"
     gene            2162..2569
                     /locus_tag="VGB93_05760"
                     /pseudo
     CDS             2162..2569
                     /locus_tag="VGB93_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
     gene            complement(3047..3229)
                     /locus_tag="VGB93_05765"
     CDS             complement(3047..3229)
                     /locus_tag="VGB93_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145647.1"
                     /translation="MIIIRGLAAGNGIISLLRISAEIALSEAAMRDDERHQGADEAIG
                     RVEERDGYGKYIDLKH"
     gene            complement(3658..>4715)
                     /locus_tag="VGB93_05770"
     CDS             complement(3658..>4715)
                     /locus_tag="VGB93_05770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145648.1"
                     /translation="YHNFIGAPRGEIPCVAIARAGDGTIACLLPFAIRRAGFLRELTW
                     LGDELCDYNAPLLAPSFATNFGQGEFAIVWRNVLTKLQADPRTSFDVVRLTKMARQAG
                     GQSNPFVAMLSTTLNASGSYVTSLTRNWDAFYAAKRSSSTRSRDRSKRKRLAECGEIV
                     FQTPQDVQGIASALDMLMQQKSAVFAERGIGNIFMRPGYVEFYRAVATDPRFNRIVHV
                     SKLEVGAETAAVNLGLVFRGRYYYVLSSYTDGSLARFGPGAIHLRELMRYAIDRGLAS
                     FDFTIGDEPYKRDWCDGVSPLYDHIATATARGALVALPLQLGQQIKRAIKQTPALWAL
                     FSKARAVLRRGEAMKRG"
BASE COUNT          923 a         1404 c         1404 g          984 t
ORIGIN      
        1 ccgtcgaaag cggcacgctg gaagtcggtg acgcggccga cctcggcgcg accctgacca
       61 gcacgacacc gatcgatgtc ttcggcacgc tcgctggcca cggcacaatc atcggcggcg
      121 cgacgatcga gaacggcggc acgctggcgc cgggcggctc gatcggcaca ctgacgatcg
      181 acggcaatct tgctttcgat ccgggcggtt tctattcggt cgcgattgca ccggggcaga
      241 actcgctgac acaagtcgat ggcagcgtga tgctcggcgg cgcgcgggtc gtcgcctcat
      301 tgagcggttt tgcgctcggc cgccataccg ggagcaccga aacgattctc accaccacga
      361 atccgctcgg tgcccgcaat cagttcaatc ccgctgtggc attcagattc gaaggaacga
      421 gcgccgagca attcgatcca cttggcgccg tcgtgctggc tgaaacgagc aacagcgttc
      481 tcctgacgtt gccggacttc gccgtcaccc tcgctctgcc atcgaatgcg ccgacgaatg
      541 cgcagcatgt cgcgggcgcg atcaatcagt tcattgtcgg tggcggcacg ttgccagtcg
      601 gcttgcaagg cctggccaat ctcaccggcg ttgctttgaa caatgccgcc aatagtctgg
      661 ccggccagac gcagggatcc ttcgttcccg tcggtttcga cgccggcacg atgtttctca
      721 atctgctgct caatcccttt gtcgacgggc gcgaggcggc gggcgaattc gattcaccct
      781 cctccggctc aaggctggcc tacgcggcgg atgaggccaa gcccgcagcc tatcaggcgc
      841 tcgccagcgc gctttcgccg gcagcgattg gctttgcgcc acacatttca ctctgggcct
      901 cggcctatgg cggcgaaggc acgatcgggg gcaatgttgc gactggcgcc tcgagcgcga
      961 cgagccagat ttatggcttc gcggccggtg cggacgatca catcgcgtcc gatacgatgt
     1021 ttggcctggc aatcggcggc ggcggcacaa actggcaatt gaatgggctc ggcggcggct
     1081 cgtccggcct gttccagatg ggcgcctacg gcacacgcct cttcggcccg gcctatgttt
     1141 ccggcgcact ggcctattcg ttacaggacg tgcgcaattc gcgcgacgcc gaggtcggaa
     1201 cgtccgatcc gctgcacggc gatttcgtcg ccaacgtcct ttcagggcgc atcgagagcg
     1261 gctatcatct gccgtttggc gcgatcacgc tgacacccta cggtgccgtg caatcgcaga
     1321 ccatgttcct gccgtcctac ggcgaagtcg gcgccgccgg cgcttcgtct ttcgcgctga
     1381 cctacgatgg ccgcacgacg accgacacgc gcaccgaagt cggcgcctgg ttcgataccg
     1441 acctcccctc actcttcagc ggcaacgtga agctttatgg ccgcgccgcc ttcgcgcatg
     1501 atttcgagaa tgaggggtcg acgacggctc tcttccagtc attgcccggt tccgggagtt
     1561 tcatcatcaa ctccgccaag cccgacgaca attccgcgct cgtgaccgcc ggcctcgaat
     1621 ataaactcgg cgccggctgg tccgtgctcg ctaaattcga cggcgaattc tccggcaaca
     1681 gcacaatcgt gggtggcaca ggtgtcctcc gaaaagtgtg gtgacaccac gaaaagatgg
     1741 ccacgacgcg agaagctgta aaatatctgc tcaggtatgc ccactttcaa gcggagcgga
     1801 tgttgatccc aaacggcaac tatggtgaag gtttttggat gccggctgcc gcggtcgagc
     1861 ggctggttgg ggccggcgtc gggaaaaagc gcgattgaga gagcggtttg acgctcttct
     1921 cgtttgataa gagtgttcgt acgtcctttt ggatccggtt ttggagcggg tagctactcc
     1981 ggcgattgtg cgccgtcgac cgaagtctga ccctttcagc cctgtggcat acggtatttt
     2041 gccggctgag gtcgtgaaga ggccttaaat ctcctcaaca catgattgac cacggcccgc
     2101 atccgctgta gaacgtaaca ggaacgccga cccttatcaa gtctgcggat ggggtcgggg
     2161 gatggccgca gcgcatctgg aaaaactgaa cgaccgtcaa cgcgcggccg tcgaacacgg
     2221 cgtcgggctc gaggacggga agctcggggg tccgctactc atcatcgccg gcgctggctc
     2281 tggcaaaacc aacactctcg cgcatcgcgt cgcacatctg atcgtcaaca atgccgatcc
     2341 gcggcgcatc cttttgatga ctttttcgcg tcgcgccgcc tccgagatgg cgcggagggt
     2401 tgaaaggatt tgcgcacgcg ttctcgatga caaagctcac gttctggctg gcgggctgac
     2461 ctgggcgggc acgttccacg gtatcggcgc ccgcatcctc cgcgagcacg ccggcgaaat
     2521 cggcctcgac ccgcagttca cgatccacga ccgcgaagat tcggcggacg ctttcgctcc
     2581 aaaggaaggc ccgacgcgct tcctgagcac gacgccgttc gtttggctcg gcgccgtttc
     2641 gtattccatt tatatggttc atgcggcagt cgaagcgtct gttaacctcg tgctccgcgt
     2701 cgtgacgcat gcaaaagaaa ttcagttgcc gcttcacgat gcaccggttc ttctagttca
     2761 aaataatgtc ggttttggtt tagcggcggt gacggttatc ctcgtcctaa ttatctctca
     2821 tttcacgttt gcttggatcg aaaagccgtg gcgcgattgg tcgaagtccg cgtggccgaa
     2881 gctgcggact aaactggcgc caaacgcacg ggctttctag tattaatcgc agcggcgaag
     2941 acgatgacga tcctatgacg attgaaccga ttgagtcgga atttgcctcg acctcccacg
     3001 gcttcattgt ttcggaggcg cccgcacaac ggcggcgcgg ctgacctcaa tgttttaaat
     3061 cgatgtattt gccataacca tcacgctctt caacgcggcc gatggcctcg tcggcaccct
     3121 gatgacgctc atcatcacgc atcgctgctt cgctcaatgc gatttcagcg gaaattctca
     3181 acaaacttat gatgccattt ccagctgcta agcctctaat aatgatcaaa gcgagaccgg
     3241 cacttattat tgcattcgat gacctgtagt tctcgcttga gctagaggta tttaatttgg
     3301 acgtgagtcg tcgaatcaaa aagggccgct tttctttcgt gccgacggac gcccacgaag
     3361 gggcggattt cgtgatcggt gtgggtcaaa cgatccctca tctttccgaa aaggcgtaga
     3421 aggacctaca aagcgggatt attaccagaa taaagaataa gcagggtctg cgtccgcttc
     3481 agcatgaatt tcggattgcg ggcgctatga gtttcctcga ttacgacgtg atcttcgcgg
     3541 atcgtgaggg aggccgtggc ggatttgatt ttctcgcgca ggcgaatgac cgagctttcg
     3601 aggttgaagg caaatgcgtc cccgcctttt tgggaggcgg cgggctgacc cttgacgtca
     3661 accgcgcttc atcgcctctc cgcgacggag cacggcgcgg gctttgctga agagcgccca
     3721 cagtgcggga gtctgtttga tcgcgcgttt gatctgctgg ccaagttgca aaggcagcgc
     3781 gaccagagcg ccgcgcgcgg ttgcagttgc gatatgatcg tagagtggcg aaacgccatc
     3841 gcaccaatcg cgtttgtaag gctcgtcgcc gatggtgaag tcgaatgaag ctagcccccg
     3901 gtctatggca tagcgcatca gttcgcgtag atggatagcg cctgggccaa agcgtgccag
     3961 cgacccgtca gtatagctcg acaacacata atagtatcgg ccgcgaaaga cgagaccaag
     4021 attgaccgcc gcggtctcgg cgccgacttc gagtttgctc acatgcacaa tacgattgaa
     4081 acgcgggtcg gtcgccaccg cacgatagaa ctccacatag cctggacgca tgaaaatatt
     4141 gccaattccg cgttcagcga agaccgcaga cttttgctgc atcaacatgt cgagcgccga
     4201 cgcgataccc tgcacgtcct gcggcgtctg aaagacaatt tcgccgcatt cagccaagcg
     4261 cttacgtttg ctgcgatcac ggctgcgcgt cgaagatgaa cgctttgcag cgtagaaggc
     4321 gtcccagttt ctggtgagcg aagttacata tgagccgctg gcattgagtg tcgttgacag
     4381 catcgccacg aacggattgg actgcccgcc cgcttgccgg gccattttgg tgagccgcac
     4441 aacgtcgaac gacgtgcgcg ggtcggcttg cagcttcgtc agtacgttac gccagacaat
     4501 cgcaaattcg ccttgtccga aattggttgc aaagctcggc gcgagcagcg gggcgttgta
     4561 atcgcagagt tcatcgccaa gccaggtcag ctcgcgcagg aagccggcgc ggcggatggc
     4621 aaacggcagc agacaagcta tcgtgccatc gcccgcacgg gcgatggcaa cgcaggggat
     4681 ctcgccgcgc ggcgcgccga tgaaattgtg atagg
//
LOCUS       DASWOG010000285         5478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_300038,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000285 DASWOG010000000
VERSION     DASWOG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300038"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VGB93_05775"
     CDS             complement(<1..1274)
                     /locus_tag="VGB93_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0145649.1"
                     /translation="MLRPWNPNLIVRAEGRGSLSVRIAQAIIEEIQRGRLTAGDSLPG
                     TRDLAKDLEVNRKTVVQAYDELAAQGWVTTDQRRGTFIARNIRDGAPEKFEPEPAQTF
                     PEARGRAVKPPLLWPREAKAGFDDGVPDQRLIPTMAIAQAYAGAARAASRQRLLGYGD
                     PRGTEALRQSISKMLNMTRGLNTTPENICVTRGSQMALYVIAHSIVAKDDTVIFEELT
                     YPPAVQAFTLAGAKVQAAKLGPEGIDLNHLEAICRRTRVRAVYLTPGHQFPTTVVLRP
                     SARLRLRALAAQFGFIVIEDDYDHEFQFDHQPMFPLASNDHAGQIIYVGSFSKVLSPS
                     LRVGYVAGPSKLIQQIGNWIGAIDRQGDPITELAIVELIESGQLRRHIKRVHAIFDRR
                     RLAFADALQNELGDFASFDTPTGGLAFWVRFA"
     gene            1707..3131
                     /locus_tag="VGB93_05780"
     CDS             1707..3131
                     /locus_tag="VGB93_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY0145650.1"
                     /translation="MAIATIPGKLKANCLGFPELLAQNIALISPTMTAALIVPVMYSN
                     TADWSWLSYALGTIMLLFVAFNLNQFAKRSTSSGSMYAYICRGLGLTAGAIGGWALIW
                     SYLGIAMAGVTGFTVFSGKLLSMMGINAPPILLFAVCVAVSWFCAWKNVNLSARAMLL
                     CEGISMVLITILGFIVMGHHGFSIDTPQFDTKTIPWSSMGLGVVVAVFSLVGFEAATA
                     FGDEAKDPLKTVPSSVYWSLIISGVFFVFVTYFEVQGTRGYSTTLDKIDQPLNILAQL
                     VHIPLLQIPLSLGAMLSFFALNLSCVSAGGRIIYALGRHGILPAATASAHNTNETPHI
                     AVTIMALMSFIVPTVATLVGKVALLDLFNDVGTCAAFGFVTVYALITVAAPVYLKKIG
                     ELKPIDIAGCVASLLLLAVPAVGSVYPVPSPPVNYFPYAFLAYLAVGAIWIAAFYRRT
                     PTAPELIKGDLDQSHARYSAVAGE"
     gene            complement(3207..4109)
                     /locus_tag="VGB93_05785"
     CDS             complement(3207..4109)
                     /locus_tag="VGB93_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145651.1"
                     /translation="MTSQTDIAAAQSAPELQMTLKRIAEALERLAPPPAPAPDLSRAD
                     AFVWQAKGGLLHPVIKVNRVEIELLRGIDQVRDILIENTERFAKGFAANNALLWGARG
                     MGKSSLVKAVHADINVRLAKTPGYRPMKLIEIHREDIEHLPDLMALLRDSPYRFLIFC
                     DDLSFDGEDTSYKSLKTVLEGGIEGRPANVILYATSNRRHLLAREMIENERSTALNPG
                     ETVEEKVSLSDRFGLWLGFHKCSQYDYIAMVFAYIAHYGLEVDRVKTTAEALEWATTR
                     GARSGRTAWQFIQDLVGRAGKTTN"
     gene            4267..4581
                     /gene="yajC"
                     /locus_tag="VGB93_05790"
     CDS             4267..4581
                     /gene="yajC"
                     /locus_tag="VGB93_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0145652.1"
                     /translation="MFSQASGGASDIFSFVAPFVLIVIIMYFLVIRPQQKSARAHSDL
                     VKNVRRGDTIVMTGGLIGKVSRVIDDNEVELEIAPNVKVRVSRAQISDVRSKGEPVKD
                     AA"
     gene            4619..>5478
                     /locus_tag="VGB93_05795"
     CDS             4619..>5478
                     /locus_tag="VGB93_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0145653.1"
                     /translation="MLRFATWKVVSILSATLLAVLVIAPSLLSPGARAALEAHVPSWL
                     PMRTIVLGLDLQGGSHVLLEVDAASVLKTEVQNLRDDTRRLLREQKIPISGGVAMLQK
                     GVQFHLLDAADGQKIMPKLSQLAQPIGGTLLGGTAAPGLDVKDTGNGTLQLLVTDDAL
                     HDKVRHAVDQSIEVLRRRVDALGTTEPNIQRQGDDRILVEVPGLQNPEKLKQILGTTA
                     KLEFRLVGQPNEDPNDVEQLDQTEEPGKIPVEKQVMVQGEDLTDAQPGFDPQTGEAIV
                     DFRFNIRGGER"
BASE COUNT         1003 a         1656 c         1692 g         1127 t
ORIGIN      
        1 tccgcaaaac gcacccagaa ggcgaggccg ccggtcggcg tgtcgaagct cgcgaaatcg
       61 ccgagttcat tctgcaaggc atcggcgaat gccagccgcc gccggtcgaa gatggcgtgg
      121 acgcgtttga tgtggcggcg caattgtccg gattcgatca attcgacgat ggcgagttcg
      181 gtgatcggat cgccttgccg gtcgatggcg ccgatccaat tgccgatctg ctggatgagc
      241 tttgacggtc cagcgacata gccgacgcgc aggctcggcg agagcacttt cgagaacgag
      301 ccgacataga tgatctgccc ggcgtggtcg ttactcgcca gcggaaacat cggctgatga
      361 tcgaactgga attcgtgatc gtaatcgtcc tcgatgacga tgaagccgaa ttgcgcggcg
      421 agcgcgcgca gccgcagccg ggcacttggg cgcaacacga cggtcgtcgg gaattgatgg
      481 ccgggcgtga gatagacggc gcgcacgcgc gtgcggcggc agatcgcttc aagatgattg
      541 aggtcgatgc cctctgggcc aagtttggcc gcctgaactt tggcgccggc cagcgtgaag
      601 gcctgcaccg cgggcggata ggtcagctcc tcgaagatca cagtgtcgtc tttggcgacg
      661 atactgtggg cgatgacgta aagcgccatc tggctgccgc gcgtcacgca gatgttctcc
      721 ggcgtcgtat tgaggccgcg cgtcatgttg agcatttttg agatcgattg acggagcgcc
      781 tcggtgccgc gcggatcgcc atagccgagc agccgctgcc gcgaggcggc gcgtgcggcg
      841 cccgcatagg cctgcgcgat cgccatggtc gggatcagcc gctggtccgg cacgccgtca
      901 tcgaacccgg ccttggcctc gcgcggccag agcaggggcg gtttgacggc gcggccgcgc
      961 gcctcaggga acgtctgcgc cggttccggc tcgaacttct ccggcgcgcc gtcgcggata
     1021 ttgcgcgcga tgaacgtgcc gcgcctctgg tcggtcgtca cccagccctg cgccgccaat
     1081 tcgtcgtaag cttgaacaac ggttttgcgg tttacctcga gatctttggc aaggtcgcgg
     1141 gtgcccggca gactgtcgcc cgcggtgagc cgcccgcgct ggatttcttc gatgatcgcc
     1201 tgggcgatcc gcacactgag actgccgcgc ccctcggcgc ggacgataag attcggattc
     1261 cacggacgta gcattctggc ccctgaagct gaggtctgga gacctggcga gggcgccagg
     1321 cacgtttcct cataagcaaa gattgtgctt gtggtcccct ctgcggtgtt ttgagccagg
     1381 gtaggcgggg ccttggggcc aagcggtttt ttagattaga ataagtgcaa aaagaagttc
     1441 cagtgtggcg cggaataagg ctgccagagc ctaaagaaag tgccgttttc tctcggaaag
     1501 cgtcaaaaca cgcgcttcag tgtgcattgc cagaagctat cgccgcgagt gggcctcctg
     1561 gaccaccttg catttaagaa ctggatgttc agggcgggcc aaggcccccc tagccttaaa
     1621 tgcaattgcg acccaacgtc gcgaggcagg aaggctcgtg accgctggcc gcaagaggtg
     1681 caaccctctt tcgaaagttt gtcgccatgg caatagcaac gatccccgga aagcttaagg
     1741 cgaattgcct cggcttccct gaactgctcg cgcagaacat cgcgctgatc tcgccgacga
     1801 tgaccgccgc gctcatcgtc cccgtcatgt attcgaacac ggccgattgg agctggctct
     1861 cctatgcgct cggcacgatc atgctgttgt tcgttgcctt caacctcaat caatttgcca
     1921 aacgttccac ctcatccggt tcgatgtacg cttatatctg ccgcggcctg ggattgacgg
     1981 cgggagccat cggcggctgg gcgctcatct ggtcttatct cggcatcgcc atggcgggtg
     2041 tcaccggctt cacggttttc tccggcaagc tattgtcgat gatgggtatc aacgcgccgc
     2101 caatccttct tttcgcggtc tgcgttgcag tttcctggtt ctgcgcctgg aagaacgtca
     2161 atttgtccgc ccgcgcgatg ctgctctgcg aaggcatttc gatggtgctg atcaccattc
     2221 tcggcttcat cgtgatgggc catcatggct tttcaatcga cacgccgcag ttcgacacga
     2281 agaccattcc gtggtcgagc atgggtctcg gcgtcgtggt tgcagtcttc tcgctcgtcg
     2341 gcttcgaggc agcgaccgcc ttcggcgacg aggcgaaaga cccgctgaag acagtgccaa
     2401 gctcggtcta ttggagcctg atcatctccg gcgttttctt cgtctttgtt acttacttcg
     2461 aagtgcaggg aactcgcggt tattcgacga cgcttgacaa gatcgaccag ccgctcaaca
     2521 tcctggcgca actggtccat atcccattgc tgcagattcc gctgtcgctc ggcgcgatgc
     2581 tgagcttctt cgcgctgaac ctgtcctgcg tcagcgcagg cggccgcatc atctatgcgc
     2641 tggggcgtca cggcattctg ccggcagcaa cggcttccgc gcataacaca aacgagacgc
     2701 cgcatatcgc ggtgacgatc atggcgctca tgtcgttcat tgtcccgacg gtggcgacgt
     2761 tggttggaaa agtcgcgttg ctcgacctct tcaacgatgt cggcacctgc gcggcctttg
     2821 gcttcgtgac cgtctatgcg ctgatcaccg tcgcggcgcc ggtctatctc aagaagatcg
     2881 gcgaactgaa gccgattgat atcgccggct gcgtcgcgtc gctgctgctc ttggcagtgc
     2941 cggcggtcgg cagcgtctat ccggtgccgt cgccgccggt gaactacttc ccctatgcct
     3001 tccttgccta tctggcagtc ggcgccatct ggattgcggc gttctaccgc cgcaccccga
     3061 cggctcccga actgatcaag ggcgacctcg accagagcca tgcacgttac agcgcggtcg
     3121 ccggcgaata acaatccacc atccgccgaa acgagaagcg cgccatcatg gcgcgctttt
     3181 caattttgag cgttgatttg gggcgttcag ttcgtcgtct tgccggcgcg accgacgaga
     3241 tcctggatga actgccaggc ggtacggcca gagcgggcgc cacgcgtcgt ggcccattcg
     3301 agagcctcgg ccgtcgtctt gacccggtca acttcaaggc cgtaatgcgc gatataggcg
     3361 aagaccatcg cgatatagtc gtactggctg catttgtgga agccgagcca gagaccgaaa
     3421 cggtccgaga gcgagacctt ttcttccacc gtctcgccgg ggttgagcgc tgtcgagcgc
     3481 tcattctcga tcatctcgcg cgccagcagg tgtcgccggt tcgacgtggc atagaggatg
     3541 acattcgccg ggcgcccttc gatgccgcct tcgagcaccg ttttcagcga cttgtaactc
     3601 gtgtcttccc catcgaagga gagatcgtcg cagaagatga gaaagcggta gggcgagtcg
     3661 cgcaaaagcg ccatcaagtc cggcaaatgc tcgatatcct cgcggtggat ttcgatgagc
     3721 ttcatcggcc gatagcccgg cgtcttggca agccgcacat tgatgtcggc atgcacggcc
     3781 ttgaccagcg aagacttgcc catgccgcgg gcaccccaga gcagagcgtt attggcggca
     3841 aatcctttgg cgaaacgctc ggtgttctcg atcaggatgt cgcgcacctg atcgatgccg
     3901 cgcagcaatt cgatctcgac gcggttgacc ttgatgacgg ggtgcaaaag gccgcccttc
     3961 gcttgccaga cgaaagcgtc ggcgcggctt aggtccggcg cgggtgccgg cggcggcgcg
     4021 aggcgctcca gggcctcggc gatgcgtttc aaggtcattt gcagctcagg cgcactctgt
     4081 gcggccgcga tatccgtctg gctggtcatt ttttctcgca ttcccaaggt gatgcggatg
     4141 taatgaaacg cgctgtctgc gtccagcagg gcgccattgc tttcgcgccc atgctggtat
     4201 agtcccggcg ccaacgaggg tgaaataccc gcctagaggg gcaacaagcc cctgaaggag
     4261 aagaagttgt ttagccaggc ttccggcggc gcgtccgaca ttttcagttt tgttgcgccc
     4321 ttcgtgctca tcgtcatcat tatgtatttc ttggtcattc ggccgcagca gaagagcgcc
     4381 cgtgcgcatt ctgatctagt caagaacgtc cgccgtggcg acacgatcgt catgaccggc
     4441 ggcctcatcg gcaaagtctc tcgcgtgatc gacgacaacg aagtcgagct tgaaattgcc
     4501 cccaatgtca aagtgcgggt atcgcgggcg cagatttcgg acgtgcgttc caaaggcgag
     4561 ccggtcaagg atgccgcctg acgcgtttgc gtctaactag aaaagataat tctcgccgat
     4621 gctgcgcttt gcgacctgga aagttgtttc gatcctatcg gcgaccctgc tcgccgtttt
     4681 ggtgatcgcc ccgagcctgt tgtcgccagg cgcgcgcgcc gcgcttgagg cgcatgtgcc
     4741 gagctggctt ccgatgcgca ccatcgtgct cggcctcgat ctgcaaggcg gctcgcatgt
     4801 cctgctcgag gtcgatgccg cctcggtcct gaaaaccgaa gtccagaatc tgcgcgatga
     4861 tacgcggcgc ctgctgcgcg agcagaaaat cccgatcagc ggcggcgtcg ccatgctgca
     4921 gaagggcgtg caattccatc tgcttgatgc ggccgacggc cagaagatca tgccgaagct
     4981 cagccagctt gcccagccga tcggcgggac tttgcttggc ggcacggcgg ctccggggct
     5041 cgatgtcaaa gatacgggca atggcacgct tcaattgctg gtgacggacg acgccctcca
     5101 cgacaaggtg cgccacgcgg tcgatcaatc gatcgaagtg ctgcgacgcc gcgtcgatgc
     5161 cctgggcacg accgagccga acattcagcg ccagggcgac gatcgtattc tggtcgaagt
     5221 gccggggctg caaaaccccg agaagctgaa gcaaattctc ggcacgaccg ccaaactcga
     5281 attccgcctc gtcggccagc caaacgagga cccgaacgac gttgagcagc tcgaccagac
     5341 cgaggaaccc ggcaagatac cggtcgaaaa acaggtcatg gtgcagggcg aggatttgac
     5401 tgacgcgcag ccgggtttcg atccgcagac cggcgaagcc atcgtcgatt tccgcttcaa
     5461 tatccgcggc ggcgagcg
//
LOCUS       DASWOG010000286         5774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_318267,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000286 DASWOG010000000
VERSION     DASWOG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318267"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGB93_05800"
     CDS             <1..325
                     /locus_tag="VGB93_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0145654.1"
                     /translation="DDIMALAIRVNDFLTGLFLGVGIRLVDFQMETGRLWEGDTMRIV
                     VADEISPDSCRLWDIKSNDKLDKDRFRRDMGGLVEAYTEVARRLGILQENEQPRASGP
                     KLVES"
     gene            429..668
                     /gene="purS"
                     /locus_tag="VGB93_05805"
     CDS             429..668
                     /gene="purS"
                     /locus_tag="VGB93_05805"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0145655.1"
                     /translation="MKANIIVTLKNGLLDPQGKAIEGALKSLDIPGIESVRQGKIFEV
                     EIVEADRAKAQALLQTACEKLLANTIVENYRIELA"
     gene            677..1363
                     /gene="purQ"
                     /locus_tag="VGB93_05810"
     CDS             677..1363
                     /gene="purQ"
                     /locus_tag="VGB93_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0145656.1"
                     /translation="MHAAIILFPGSNREGDVFRALASVTRQKPSIVWHGEPELPAGTD
                     LVVLPGGFSYGDYLRCGAIAARSPVMDAVRAHAKRGGRVLGICNGFQIACEAGLLPGV
                     LMRNTNLRFACHMQHLRVERADTGFTAQYQKGQVIKVAIAHGEGNYTADSETLARLEG
                     EGRIAFRYCDAAGALGGAANPNGSVHDIAGIYSANLNVLGLMPHPENLIDPLVGGIDG
                     RALFESLVAA"
     gene            1397..2653
                     /locus_tag="VGB93_05815"
     CDS             1397..2653
                     /locus_tag="VGB93_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY0145657.1"
                     /translation="MTTHERKISAAIDEAKLDKLAEVVVKVGLNLQEGQDLFLTAPVT
                     ALAFVRRIVEHAYKAGAGLVTPMLADEQMTLARFRFAHDASFDRAPGWLYEGIGKAFA
                     ANTARLAIVGDNPMLLAGEDPAKVSRANKANSIAYKPALEKIAGFDINWNIAAYPGAA
                     WAHQVFPEDEPDVAVAKLSDAIFAASRVDKADPIAAWAAHNAALRSRTQGLNGQRFAA
                     LHYTGPGTNLTIGLADGHEWEGGASVAKNGVVCNPNIPTEEVFTTPHAHRVDGTVVST
                     KPLSYQGTLIDRIAVRFEAGRIVEATAAHGQDVLRKVLETDDGARRLGEVALVPHSSP
                     ISASGLLFFNTLFDENAASHIALGQCYAKCFIDGDKLTPEQIAAQGGNTSLIHIDWMI
                     GSDKIDIDGIKTDGSRVPVFRKGEWA"
     gene            complement(2683..3081)
                     /locus_tag="VGB93_05820"
     CDS             complement(2683..3081)
                     /locus_tag="VGB93_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145658.1"
                     /translation="MTKSEELGLMLNRYYNLEEKRQLVESIIEPAKAYLNGKAGSIET
                     TRALSQFVGRDRDLDRLLFPLVGVNSETDALPVGNVRKYWDTEALKQEDIKIAAAEAR
                     YASIVSDACHALVASLVPLLSDLRDQHPKS"
     regulatory      3177..3302
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3291..4316
                     /locus_tag="VGB93_05825"
     CDS             3291..4316
                     /locus_tag="VGB93_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145659.1"
                     /translation="MSDFPKKIRILGAGVAGLTAAYEFAAAGQTVEIVERQAAPGRGC
                     SYFAGGMLAPWCEAESAPPIVIEWGREGLAFWTETLEVATVNGSLLVAPARDRADLIR
                     FSRRTSGHEWFDADQLAACEPHLGGRFDRALFFAGEAHLDPRAAIEKLVARLREFERV
                     TLRFDTPAEAITDEADWTIDCRGFAARDALPDLRGVKGEMLVVKTDEISFQRPVQLLH
                     PRHPVYVVPRGDGRFMVGATAVENEERPRITARSMLELLSAVYALHPAFGEAEIVETG
                     ADLRPTFPDNLPRIRREGRTIFINGLYRHGFLLAPALARRAVAVALKDETFPEVMDAN
                     PRQRQRA"
     gene            4285..4482
                     /gene="thiS"
                     /locus_tag="VGB93_05830"
     CDS             4285..4482
                     /gene="thiS"
                     /locus_tag="VGB93_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0145660.1"
                     /translation="MQIRVNGKEHDVAATTLAALLAELDYQDRLVATAVNQTFVRAID
                     RPATPLRTGDAVEILVPRQGG"
     gene            4495..5295
                     /locus_tag="VGB93_05835"
     CDS             4495..5295
                     /locus_tag="VGB93_05835"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0145661.1"
                     /translation="MQNTPFEVYGVTLGSRLLIGTAQYPSPAVLADAVKASGAEVVTV
                     SLRREAGKGRAGESFWSLIRDLGVKVLPNTAGCRSVKEAVTTAEMAREVFATDWIKLE
                     VIGDDDLLQPDVFGLVEAARILSRAGFKVFPYTTEDLLVAERLVGTGCEVLIPWGAPI
                     GSGRGLNNVFGLQALRKNFPSMPLIIDAGIGTPAHAAAAMELGYDAVLLNTAIAKAGD
                     PVAMARAFAFGIEAGRLGYCADPILPRDMAEPSTPQIGRAFADLPKRA"
     gene            5288..>5774
                     /locus_tag="VGB93_05840"
     CDS             5288..>5774
                     /locus_tag="VGB93_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0145662.1"
                     /translation="MLDRFYPIVDSADWVARLLTCGARFVQLRVKDQPIEDVRREIRT
                     ARTLCLAAGAELVVNDYWQEAIDLSCTHVHLGQGDLETADVAMIRGANIKIGISTHDE
                     AELARALALKPDYIALGPIYPTILKAMAFAPQGLDRIGEWKKKIGTIPLVAIGGITLE
                     RA"
BASE COUNT         1096 a         1727 c         1779 g         1172 t
ORIGIN      
        1 cgatgacatc atggcgctcg ccatccgcgt caatgatttc ctgaccgggc ttttcctcgg
       61 cgtcggcatt cgtcttgtcg atttccaaat ggaaactggg cgcctatggg aaggcgatac
      121 catgcgcatc gttgtcgccg atgaaatctc ccccgattca tgccggctct gggacatcaa
      181 gtcgaacgac aagctcgaca aggaccgttt ccggcgcgac atgggcggtc tcgtcgaagc
      241 ttatacggaa gtggcgcgcc ggctcggtat cctgcaggag aacgagcaac cgcgggcgtc
      301 gggcccgaag cttgtcgaat cttgagcttc actttggctt aaaccaaaac cccgccgctg
      361 acgcggcggg gttttcttat gcctgccagc cgatatagta cgcgtgggca cgacacgagg
      421 catcgatctt gaaagccaat atcatcgtta cgctgaaaaa tgggcttctc gatccgcaag
      481 gcaaagcgat cgaaggcgcg ctgaagtcgc tcgatattcc tggcatcgaa agcgtgcggc
      541 aaggcaagat tttcgaggtt gaaattgtgg aagcggaccg cgcgaaggcg caggcgctgt
      601 tgcagacggc gtgcgagaaa ttgctcgcca acacgatcgt cgagaactat cgcatcgaac
      661 tcgcctgagg tcttcattgc acgctgcgat cattctcttt cccgggtcca atcgcgaagg
      721 cgatgtattt cgcgcgctcg ccagtgtaac caggcaaaag ccctcgattg tctggcatgg
      781 cgagcctgaa cttccggctg gcacagacct tgtggttctg cccggcggct tttcctacgg
      841 tgattatctg cgctgcggtg ccatcgcggc gcgctcgcct gtgatggacg cagtccgggc
      901 gcacgcgaag cggggcggcc gtgtgctcgg catctgtaat ggcttccaga tcgcttgcga
      961 agcgggcctg ctgccgggcg ttctcatgcg caacacgaat ttgcgcttcg cctgccatat
     1021 gcagcatctg cgcgtcgaac gcgctgacac gggctttacc gcgcaatatc aaaagggtca
     1081 ggtgattaag gtcgccatcg cgcatggcga aggcaattac accgccgata gcgagacgct
     1141 ggcgcggctc gaaggcgagg ggcgcatagc cttccgctat tgcgatgctg ccggcgcgct
     1201 cggcggcgcg gccaatccga atggctcggt gcatgacatc gccggcatct attccgcaaa
     1261 tctgaacgtg ctcggactga tgccgcatcc ggaaaatctg atcgacccgc tcgtcggtgg
     1321 catcgacggg cgggcgcttt tcgaaagcct ggtcgcggct taagccgcct gtctcgtctc
     1381 gaacccgcgg agccctatga caacgcacga gcgcaaaatc tctgccgcca tcgatgaagc
     1441 gaagcttgac aagctcgccg aggtcgtcgt caaggttggg ctgaatctgc aagaaggtca
     1501 ggacctattt ctgacagcgc cggtcacggc gcttgccttc gtgcggcgca tcgtcgaaca
     1561 cgcttataaa gccggcgccg gcctcgtgac gccgatgctc gccgacgagc agatgacatt
     1621 ggcgcgtttc cgctttgcac atgacgcgag tttcgatcgc gcgcccggct ggctgtatga
     1681 aggcatcggc aaagccttcg ccgcgaacac cgcgcggctc gctattgtcg gcgacaatcc
     1741 aatgctgctt gcgggcgaag acccggccaa ggtctcgcgc gccaacaagg ccaattccat
     1801 cgcctacaaa ccggcccttg aaaagatcgc gggctttgac atcaattgga acatcgctgc
     1861 ttatccgggc gcggcctggg cgcatcaggt ttttccggag gacgagccgg acgttgcggt
     1921 cgcgaaacta agcgacgcga ttttcgccgc ctcgcgcgtc gacaaggccg atcccatcgc
     1981 ggcctgggcc gcgcataacg ctgcgctgcg cagccgcacc caagggctca acggccagcg
     2041 cttcgccgcc ctgcattaca cggggccggg cactaatctg acgattggtc tcgcggatgg
     2101 ccacgaatgg gaaggcggcg cgtcggtcgc caagaacggc gttgtctgca atccgaatat
     2161 cccgactgaa gaggtcttca cgacaccgca cgcacatcgc gtcgacggca ctgtcgtcag
     2221 caccaagccg ctctcttatc agggcacgct gatcgaccgg atcgcggtgc gtttcgaggc
     2281 gggccgaatc gtcgaggcga cagccgcgca tggtcaggac gtgctgcgca aggttctcga
     2341 aacagatgat ggcgcccggc ggcttggcga agtcgcgctg gtgccgcatt cctcaccgat
     2401 ctcggcgagc ggtcttctgt tcttcaacac tctgttcgat gagaacgcgg caagccacat
     2461 cgcgcttggt cagtgctatg ccaaatgctt catcgacggc gacaagctga cgcccgaaca
     2521 gatcgcggcg cagggcggca atacgagcct tatccacatc gactggatga ttggctccga
     2581 caagatcgac atcgacggca tcaagaccga tgggtcgcgc gttccggtct tccgcaaggg
     2641 cgaatgggcg taattcggcg ataccgcgat cacactagaa gctcaacttt tcgggtgttg
     2701 atcgcgaagg tccgataaaa gaggtaccaa cgaagccacc aaggcgtgac acgcgtctga
     2761 gacgatgctc gcatagcgag cctctgccgc cgcgatctta atatcttctt gcttcaaggc
     2821 ctcggtatcc caatatttcc gaacgttgcc tacgggcagc gcgtcggtct cgctattcac
     2881 accgaccaag gggaacagaa gtctgtctaa atcgcggtcg cggccaacga attgagaaag
     2941 agcccgcgtc gtttcgatag agccagcctt gccattgaga tatgccttcg ccggttcaat
     3001 tatcgattcg actagctgac gtttttcttc aagattgtag tagcgattta gcatgagtcc
     3061 caattcctcg gacttcgtca ttagcgggga tgccaactct gaggagccgc gcatcctaat
     3121 cggcaaggca gtgacgagcc aattgttgac ctaccggggc gttcggccta tctaacatct
     3181 gtgcctaggg gatccccgtc agggggctga gaggctggca gcgcttcggc tcagaagcgg
     3241 cccggcgacc cttcgaacct gatccagttt gtactggcgt agggatggtc atgagcgatt
     3301 ttccgaaaaa aatccgcatt ctcggcgccg gcgtcgccgg cctcacggca gcctatgaat
     3361 tcgccgccgc cggccagacg gtcgaaatcg tcgagcgcca agccgcgccc ggccgcggct
     3421 gttcgtattt tgccggtggc atgttggcgc cctggtgcga agctgaaagt gcgccgccaa
     3481 ttgttatcga atggggccgc gaaggtctcg ccttctggac cgaaacgctc gaagtagcga
     3541 ccgtcaacgg cagcctgctt gtcgcgccag cacgtgaccg tgccgatctc atccgtttct
     3601 cgcggcggac gtctgggcat gaatggttcg atgccgatca gcttgcggcc tgtgagccgc
     3661 atcttggcgg ccgcttcgat cgcgcgctgt ttttcgcggg cgaagcgcat ctcgacccgc
     3721 gggcggcgat cgaaaagctc gttgcgcggc tgcgtgaatt cgagcgcgtg acacttcgtt
     3781 tcgacacccc agccgaggcg atcaccgacg aagccgattg gacgatcgat tgtaggggtt
     3841 ttgccgcgcg tgatgcattg cctgatttgc gcggcgtgaa gggcgagatg cttgtcgtca
     3901 aaaccgacga gatttctttt cagcggccgg tgcaattgct gcatccgcgc catccggtct
     3961 atgtcgtgcc gcgcggcgat ggccgcttca tggtcggcgc gacagcggtc gaaaacgaag
     4021 agcgtccgcg catcacggcg cgttcgatgc tcgaactttt aagcgcggtt tatgcgctgc
     4081 atccggcttt tggcgaggcc gagattgtcg aaaccggcgc cgatctgcgt ccaacttttc
     4141 ctgataacct tccacgcata aggcgcgagg gccgcacaat ctttatcaac gggctttatc
     4201 gacacggctt tcttctggcg ccggcattgg cgcgccgcgc cgtcgccgtg gcgctcaagg
     4261 atgaaacttt tccggaggtg atggatgcaa atccgcgtca acggcaaaga gcatgacgtt
     4321 gccgcgacga cgctcgcggc tttgcttgct gaacttgact atcaggatag actcgttgcg
     4381 acagcggtga accaaacctt tgtccgcgcg attgatcgcc cggccacgcc gcttcgcacc
     4441 ggtgatgccg tcgaaatcct cgtgccgcgg caagggggct gatcaggatt gtccatgcag
     4501 aatacgccct ttgaagttta cggtgtgacg cttggctcgc gtttgttgat cggcacggcg
     4561 caatatccct cgccggccgt tcttgccgac gcggtcaaag cgtccggcgc cgaagtcgtc
     4621 acggtctcgc tgcggcgtga ggcgggcaag ggccgcgcgg gcgagagctt ctggagcttg
     4681 atccgcgacc ttggcgtcaa ggttctgccg aatacggccg gttgccgcag tgtcaaggaa
     4741 gctgtgacga ccgccgagat ggcgcgcgag gttttcgcca ccgactggat caagctcgaa
     4801 gtgatcggcg acgatgatct gttgcagccg gatgtcttcg gacttgtcga ggcagcaagg
     4861 attctttcgc gcgcgggctt caaggtattt ccctacacga cggaagattt gctcgtcgcc
     4921 gaacggcttg tcggcacggg ctgcgaagtc ctgattccct ggggggcgcc gatcggctct
     4981 ggccgcgggc tcaacaatgt cttcgggctc caggcgttgc gaaagaattt tccgtcgatg
     5041 ccgctcatca tcgatgcggg aatcggcacg ccggcacatg ccgccgcggc gatggaactt
     5101 ggctacgatg cggtcctatt gaacactgcc atcgccaaag ctggcgatcc tgtcgcgatg
     5161 gcccgcgcct tcgcgttcgg catcgaggcg gggcgtctcg gctattgtgc cgatccgatt
     5221 ttaccgcgtg acatggcgga gccgtcgacg ccgcagatcg gccgcgcctt cgcggacttg
     5281 cccaaacgtg cttgatcgct tctaccccat tgtcgatagc gcggactggg tcgcgcgact
     5341 cctcacatgc ggcgctaggt tcgtgcaatt gcgagtcaag gatcagccga tcgaggacgt
     5401 caggcgtgag atcagaaccg cacggacgct ctgtctcgct gcgggcgccg aactggtcgt
     5461 caacgattat tggcaggagg cgatcgacct cagttgcacg catgtccatc tcgggcaagg
     5521 cgatctcgaa accgccgatg tggcaatgat ccgcggcgcg aatatcaaaa tcggtatttc
     5581 gacgcatgac gaggccgagc ttgcgcgcgc gctcgcactg aagccggatt acatcgcgct
     5641 cgggccgatt tatccgacga tcctgaaagc gatggctttc gcaccgcagg ggctcgatcg
     5701 catcggcgag tggaagaaaa aaatcggcac cattccgctc gtggcgattg gcgggattac
     5761 gctggagcgc gccc
//
LOCUS       DASWOG010000287         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_42688,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000287 DASWOG010000000
VERSION     DASWOG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42688"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VGB93_05845"
     CDS             complement(<1..335)
                     /locus_tag="VGB93_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEY0145663.1"
                     /translation="MSFQGRVLKPALFALSAAAMLAGAGTAKAAQVGLLECNVAPSVG
                     LIITSSKALACRFTRTYAPTDYYVGTITNFGLSIGATGAGRIVWGVFAATHELGHRAL
                     AGHYAGASA"
     gene            complement(457..1359)
                     /gene="hemF"
                     /locus_tag="VGB93_05850"
     CDS             complement(457..1359)
                     /gene="hemF"
                     /locus_tag="VGB93_05850"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY0145664.1"
                     /translation="MTANTNAIAQQKTEAHAWFEALQARICAAFEALERDCAGPFAAA
                     DMAPGRFVVTPWHRKDASGADGGGGKMGLLHGRVFEKAGIHTSAVHGTFAPEFAKQIH
                     GADSDPHFWAAGISLIAHPWNPNVPTVHMNTRFVVTQKAWFGGGADLTPVLDRRRTQT
                     DGDSIAFHAGFEAACRRHAEVADYQRFKEWCDEYFFLKHRNEPRGIGGIFYDHLASPL
                     GFAADFAFTRDVGGTFLSLYPEIVRRNFANAWSEADRDEQLVRRGRYVEFNLLYDRGT
                     IFGLKTGGNVDSILSSMPPLVKWP"
     gene            complement(1356..1847)
                     /locus_tag="VGB93_05855"
     CDS             complement(1356..1847)
                     /locus_tag="VGB93_05855"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0145665.1"
                     /translation="MTQHSGPALTLALYQPDIPQNCGTMLRLCACLGIDAAIIEPAGF
                     PSSDRAFRRAGMDYLDHVAIARHIGFAAFLTWRKAQARRLVLLSTKGAQDYCDFHYAP
                     GDILLVGRESAGVPEDVRAAADASVRISIRAPLRSLNVAVAAAMVLGEAVRQIGKEDL
                     KTG"
BASE COUNT          384 a          597 c          575 g          336 t
ORIGIN      
        1 ttggcactgg cgcccgcgta atggccggcg agtgccctgt ggccgagttc atgcgttgcg
       61 gcgaacacgc cccaaacgat gcggccagcg ccggttgcgc cgatcgacaa gccgaaatta
      121 gtgattgtgc cgacataata atcggtcggc gcgtaggtgc gagtgaagcg gcaggccagc
      181 gccttgcttg atgtgatgat gagtccgacg ctgggcgcca cattgcattc cagaaggccg
      241 acttgcgccg ccttggccgt gccggccccc gccagcatcg ccgccgcgct gagtgcgaaa
      301 agcgccggct tgagcacccg tccctgaaag ctcataaata atctccctct taacgtcgta
      361 tgaaaatagc cgagtccacg ctgccttcca cgcaagctgg acttattcgt gtccacgccc
      421 caccggcggc gggtttgcgg ccagattatg gaagcgtcac ggccatttga ccagcggcgg
      481 catagacgaa aggatcgaat cgacattgcc gccagtcttc aggccgaaga tcgtgccgcg
      541 atcgtaaagc aggttgaact cgacatagcg gccgcggcgc acaagctgct catcgcggtc
      601 ggcctcactc caggcgttgg cgaaattcct gcgcacgatc tcgggataga gcgagaggaa
      661 cgtcccgccg acatcgcggg taaaggcgaa atctgccgca aagccgagcg gcgaggcgag
      721 atgatcgtag aaaatgccgc cgatgccacg cggctcgttg cgatgtttca ggaagaaata
      781 ctcgtcgcac cattccttga atcgctgata gtccgcgacc tcggcatgac gccggcaggc
      841 cgcctcgaaa cccgcatgga aagcaatcga atccccgtcc gtctgcgtgc gccggcgatc
      901 gagcaccggc gtcagatcgg cgccaccgcc gaaccaggcc ttctgcgtca cgacgaagcg
      961 cgtgttcatg tgaacggtcg ggacattcgg gttccaggga tgtgcgatga gcgaaatgcc
     1021 ggcggcccaa aaatgtggat cggaatcggc gccgtggatc tgcttggcga attccggtgc
     1081 gaacgtgcca tgcaccgccg atgtatgaat gccagccttt tcaaagaccc ggccatgcag
     1141 caagcccatc ttgccgccgc cgccatcggc gccagaggcg tccttgcgat gccagggcgt
     1201 aacgacaaaa cggccaggcg ccatatcggc cgcagcaaac gggccggcgc aatcgcgttc
     1261 aagcgcctcg aaggcggcgc atatccgggc ctgcaaagcc tcgaaccagg catgggcttc
     1321 ggtcttttgc tgcgcgatgg cgttcgtatt tgccgtcatc cggttttcaa atcctctttg
     1381 ccaatctgcc tgacggcctc accgagcacc atggccgccg cgaccgcgac gttcaaggag
     1441 cgtaacggcg cccggatcga gatgcgcacg ctcgcatccg ccgccgcccg cacgtcttcc
     1501 ggcacgcccg ccgattcgcg accgacgagt agaatgtcgc ctggcgcata gtgaaagtcg
     1561 caataatcct gcgcaccctt tgtcgacagc aacacgagcc ggcgcgcctg cgctttgcgc
     1621 caggtcaaga aagccgcgaa tccgatatgc cgggcgatcg ccacatgatc gaggtaatcc
     1681 atgccggcgc ggcggaaggc gcggtcgctc gatggaaatc ccgccggctc gatgatcgcg
     1741 gcatcgatcc cgagacaggc acacagacgc agcatcgttc cgcaattttg cggaatgtcc
     1801 ggctgataga gcgctaaggt caatgccggg ccagaatgtt gcgtcatcgt tcaagcaccg
     1861 tgcgcgcttt tcatttgatt tcgtgtcaag tg
//
LOCUS       DASWOG010000288         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_134928,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000288 DASWOG010000000
VERSION     DASWOG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134928"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1881)
                     /locus_tag="VGB93_05860"
     CDS             complement(91..1881)
                     /locus_tag="VGB93_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0145666.1"
                     /translation="MATLDLAAIGNCAIASLIDRDARHVWFCFPRLDGDPVFSALING
                     EDPQQGFMDVVVCDAVETTQRYIRNTAVLETIITDKNGGQVRIVDFAPRAPRFGRSFR
                     PPMLVRRIEPLTKRVRLKVRIRPHFNYGATRPTISFGSNHVRYTGGQMTLRVTTDMAI
                     AYLLEEAEFALDKPVNLFIGPDESVPEAPDTLAQSFLAATIEDWQAWVRGLSIPFEWQ
                     NEVIRSAITLKLCSCEDTGAIVAALTTSIPEAPHTARNWDYRFCWLRDAFFTVGALNR
                     LGATRTMENFIRFIIDAVLRADDVAIAPLYPISTSTSSEERLASALAGYQGMGPVRIG
                     NAAVSQIQNDAYGSIILTAAQIFWDARLTYKGLATLYQQLRGVGRLAARCALTPDAGP
                     WEYRGRASIHTYSAAMCWAALHRLALIAERVGESAEAQEWEALATKLRTEILNRATMP
                     EGWISGVLDAPFVDATCLLLAEVGLLPANDPRVVKTLDVIGQRLVRDGFVMRYDEADD
                     FGHPETAFLVCTFWYVDALALAGRRDQAREIFNNVLAVRNGAGLLSEDVMAGTRTLWG
                     NFPQAYSHVGLIHSAARLSRSWEEALWRAS"
     gene            complement(1894..2616)
                     /gene="otsB"
                     /locus_tag="VGB93_05865"
     CDS             complement(1894..2616)
                     /gene="otsB"
                     /locus_tag="VGB93_05865"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0145667.1"
                     /translation="MRDNRPSAPPTPARNWALFLDLDGTLLDIAATPSSVIVPRDLIS
                     DLQGAARALNGAVAIVSGRALADIDRLLSPLRLAGGSEHGVVVRLPDGTEEGMGQPVP
                     ISWTQAFIDLQEVYPGVATEVKPHNVVAHFRNAPEREGDVMRLAQELVARNPRDFELI
                     EAKMAVEIRPRAINKARPVERLMSMPPFKGRFPVFVGDDVTDEDGFEAARAHGGLGLH
                     VATRFDGQPQNVRDWLKRVADL"
     gene            complement(2866..3603)
                     /locus_tag="VGB93_05870"
     CDS             complement(2866..3603)
                     /locus_tag="VGB93_05870"
                     /inference="COORDINATES: protein motif:HMM:NF021376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HEY0145668.1"
                     /translation="MSPEERQLLTGLFDRIRASANTPRDPEAEALIADAVKTQPYSPY
                     FLAQAVIVQNQALEAANQRLNELQAQINAQQPQGQPAGGGFLSSLGSIFGGPSAPPEP
                     RPQQPLPGGPWGQSPQGQAPRSYPPQPSYPPQAPWGAPPVGGGGGGFLSGALQGAAGV
                     AGGVLLADSIRSLFGGHGGGFGGLGIGTGVGGLGSGLGGGETVVNNYYDDDGSKSGAD
                     SVDIYDDSNNSTDNFDTGNDNSGSFDV"
     gene            3744..>5071
                     /gene="hisS"
                     /locus_tag="VGB93_05875"
     CDS             3744..>5071
                     /gene="hisS"
                     /locus_tag="VGB93_05875"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0145669.1"
                     /translation="MNDIKKTPRTQAKLPRGFGDRGPAEIAATERMLAAIKESYRLYG
                     FEAVDTPFIEYTEALSKFLPDLDRPNEGVFSFQDDDEQWLSLRYDLTAPLARYVAENY
                     DRLPKPYRSFRAGYVFRNEKPGPGRFRQFMQFDADTVGTSSVAADAELCMMAADTLER
                     LGVQRGDYVIKVNNRKVLDGVLEAIGLGGDENTGRRLTVLRAMDKFDKFGDEVRLLLG
                     NGRKDESGDFTRGANLDEDQQHEIMTWLHSNAQLVDVSSLRENLRADAALLPEAPQNA
                     ISNDETLKVFRGHFGNNEKVEKGLDELQAISDICSAAGFGTRRIRIDPSVVRGLEYYT
                     GPVFEAELTFEIVEEDGRPVRFGSVGGGGRYDGLVGRFRGEDVPATGFSIGVSRLYAA
                     LKAINSPVVAGAQQRGPVIVLVLDQAELARYQSFVSMLRNAGIAAELYLGS"
BASE COUNT         1007 a         1517 c         1584 g          963 t
ORIGIN      
        1 gcgcgccttg cgtgaaaaca gctttcaacg cgaccgtcag gccgccagcc ttcgcgcctt
       61 gctacggcaa gggcacgcga tttgtgacga ttacgacgcg cgccataaag cttcctccca
      121 gctgcgcgaa agccgcgccg ccgaatgaat gagtcccaca tgtgagtagg cttgtgggaa
      181 attgccccag agcgtccgcg ttcccgccat cacatcctcg gagagaagac cggcgccgtt
      241 gcggacggct aaaacattgt tgaagatctc ccgcgcctga tcgcgccggc cggccagggc
      301 gagtgcatcg acgtaccaga aagtgcagac gagaaatgcc gtttccggat ggccgaagtc
      361 gtccgcctcg tcatagcgca tcacgaaacc gtcgcgcacg agtcgctgtc cgatgacatc
      421 gagcgttttc acaacgcgcg gatcatttgc cggcagaagc ccaacctccg ccaggagcag
      481 acaggtggca tcgacgaacg gcgcatcgag cacgccggaa atccagccct ccggcattgt
      541 cgcgcgattc aaaatctccg tgcgcagctt ggttgcgagt gcttcccatt cctgggcctc
      601 ggcggattcg ccgacgcgct cggcgatgag ggccagacga tgcagcgccg cccagcacat
      661 ggccgccgaa tacgtatgga tgcttgcccg tccgcgatat tcccacggcc cggcatcggg
      721 cgtaagcgca cagcgcgccg cgagccggcc gacgccgcgc aattgctgat aaagcgtcgc
      781 caggccttta taggtcagcc gcgcatccca gaaaatttgc gcggcggtga gaatgattga
      841 gccgtaggcg tcgttctgga tttgcgagac agcggcattg ccgatgcgca ccggtcccat
      901 gccctgatag ccggccagcg ccgacgccag ccgctcctcg gagcttgtcg aggtcgagat
      961 cggatagagc ggcgcaatcg caacatcatc ggcgcgaaga accgcgtcga tgatgaaacg
     1021 gatgaaattt tccatcgtcc gtgtcgcgcc gagccgattc aaggcgccca cagtgaagaa
     1081 ggcgtcgcgc agccagcaga aacgataatc ccaattgcgc gccgtgtgcg gcgcctcggg
     1141 aatggaggtc gtcaaagcgg cgacgatggc gccggtatct tcgcagctac agagcttcag
     1201 cgtgatcgcc gagcggatga cctcgttctg ccattcgaac ggaatggaga gaccgcggac
     1261 ccaggcctgc caatcttcaa tcgtcgcggc gagaaaactt tgtgccagtg tgtcgggtgc
     1321 ttccggcacg gattcgtcag ggccgatgaa aagattgacc ggcttgtcga gcgcgaattc
     1381 ggcttcttct aaaagatacg cgatcgccat gtcagtggtc acgcgcaagg tcatctggcc
     1441 gccggtatag cgcacatgat tgcttccaaa actgatggtc ggtctggtcg cgccataatt
     1501 gaaatgcggg cggatgcgga ccttaagccg tacgcgtttg gttagcggct cgatgcggcg
     1561 gacgagcatg ggtggccgga agctgcggcc gaaacgcggt gcgcgcggtg cgaagtcgac
     1621 aatgcgaacc tggccaccgt ttttgtcggt aatgatcgtc tcgagcacag ccgtgttgcg
     1681 gatatagcgc tgcgtggttt cgacggcgtc gcagacgacg acatccataa agccctgctg
     1741 cgggtcctcg ccattgatga gcgccgaaaa gaccggatcg ccgtcgagac gcggaaagca
     1801 gaaccacacg tgccgggcgt cgcggtcgat aaggcttgcg atggcgcaat tgccgatagc
     1861 cgcgagatca agcgtagcca tggaccgggc gcctcagagg tcggcaacgc gtttcagcca
     1921 gtcgcgcaca ttctgcggct ggccgtcgaa tcgtgttgcg acatggaggc caaggccgcc
     1981 atgcgcccga gcggcttcga atccgtcctc gtcggttaca tcgtcgccga caaagaccgg
     2041 gaaacggcct ttgaaaggcg gcatagacat cagacgctca acagggcgcg ccttatttat
     2101 cgcgcgcgga cgaatttcca ccgccatctt ggcctcgatc aattcgaaat ctcgcggatt
     2161 gcgtgcgaca agttcctgcg caagtcgcat tacatcgcct tcgcgctctg gcgcgttgcg
     2221 aaaatgcgcg acgacattat gcggtttcac ttctgtcgcc acgcccggat agacctcctg
     2281 caagtcgata aaggcctggg tccagcttat gggaacaggt tgacccattc cttcttcggt
     2341 gccgtccggc aagcggacca caacgccatg ttcgctgccg cccgctaacc gcaaagggct
     2401 cagaaggcgg tcgatatcgg ccaaggcccg gccactgaca atcgcgacgg cgccgttcag
     2461 cgcacgcgcg gcaccctgca ggtcgctgat aaggtcgcga gggacgatga cagagctggg
     2521 agtcgccgcg atatcgagca gggttccgtc taggtcgaga aacagcgccc aattccgtgc
     2581 cggtgttggc ggcgcggaag gtctgttatc ccgcaaaaga ctctccataa atcacgcccc
     2641 cggctgacgg tgctcattgg ctatccggcc gcgaccctgg cgggccatca tgagttgccg
     2701 gtatgagcga tccgcgcgcc aatgcataac gcacccgcac gataacagtc gggcggcaaa
     2761 atcgttccgg tctagctgtc agaaatttgc gtgccggagt aagaaagaaa aagcctcgcc
     2821 ggcggacggc gaggctaatt ggattgtggc cgttggatgc ggcgttcaga cgtcgaacga
     2881 gccgctattg tcgttgccag tgtcgaaatt atccgtgctg ttgttcgagt cgtcgtagat
     2941 atcgacgcta tcggcgccgc ttttgctgcc gtcgtcgtca taataattat tcacgaccgt
     3001 ctcgccgccg ccaaggccgc ttcccaagcc accgacgcca gtgccaatgc cgaggccgcc
     3061 gaaaccgcca ccgtggccgc cgaaaaggct gcggatggaa tcagccaaca gcacgccacc
     3121 ggcaacaccc gccgcgccct gcaaggcgcc gctcaggaaa ccgccgccac cgccgccgac
     3181 gggcggagcg ccccacggcg cttgcggggg ataggacggt tgtggcgggt aggagcgcgg
     3241 cgcctgccct tgtgggcttt ggccccaggg gccgccgggc agcggctgct gcgggcgtgg
     3301 ctcgggcggt gccgaagggc cgccgaagat cgaaccgaga ctgctcagaa aaccgccgcc
     3361 ggccggctgg ccctgcggct gctgcgcgtt gatctgcgcc tgcaattcgt tgagccgctg
     3421 attggcagct tccagcgcct gattctggac gatgaccgcc tgtgccagaa aatagggcga
     3481 atagggttgg gttttgacgg cgtccgcaat caacgcctcg gcttcggggt cgcgcggcgt
     3541 gttggccgac gcacgtatgc ggtcgaaaag accggtgagc aactggcgtt cttcgggtga
     3601 catgactttc tcccgtggtt cgggtttccc cgcggcacag atatgggagg cgattgcggc
     3661 gccaccaagg ggtgagatct ccgcggccag ctttcgcttc tctctttgat agcttagaag
     3721 cgccgcgaag aggtctgcgc agcatgaatg acattaaaaa gacgccacga acccaggcta
     3781 aattgccccg cggtttcggt gatcgcggcc cggccgagat cgccgcgact gagcggatgc
     3841 tggcggcgat caaggagagt taccggctct atggtttcga ggccgtcgac acgccgttca
     3901 tcgaatatac cgaggcgctg agcaaattcc tgcccgatct cgatcggcca aacgaaggcg
     3961 ttttttcgtt tcaggacgat gacgagcaat ggctgtcgct gcgctacgat ctgaccgcgc
     4021 cgctcgcgcg ctacgtcgca gagaattacg acaggctgcc gaaaccctat cgctcgtttc
     4081 gtgccggcta tgtgttccgc aacgagaagc cggggccggg acgcttccgc cagttcatgc
     4141 aattcgatgc cgatacggtc ggcacaagct ccgtcgcggc ggatgccgaa ctctgcatga
     4201 tggcggcgga tactttggag cggctcggcg tccagcgcgg cgattacgtc atcaaggtca
     4261 acaaccgcaa agtgctcgat ggtgtactcg aagcgattgg cttaggtggc gacgagaata
     4321 cagggcgcag gctgaccgtg ctgcgggcaa tggacaagtt tgacaagttt ggtgatgagg
     4381 tccgccttct tcttggcaac ggccgcaagg atgaatcagg cgatttcaca agaggcgcca
     4441 atctcgacga agatcaacaa cacgaaatta tgacgtggtt gcactccaat gcgcagcttg
     4501 ttgatgtttc cagcttgcga gaaaatctac gagccgacgc cgcgttgctg ccagaagcac
     4561 ctcaaaatgc catttcaaac gatgagacac ttaaggtttt tcgtgggcac tttggtaata
     4621 atgaaaaagt tgaaaagggc ttggatgagt tgcaagccat ctccgacata tgctctgcgg
     4681 ctggttttgg aacgcgtcgt atccgcatcg atccatccgt cgtgcggggt ctcgaatatt
     4741 ataccggccc cgtctttgaa gccgaactca cgttcgagat tgtagaggag gatggtcgcc
     4801 ccgtccgctt cggctcggtc ggcggcggcg gccgctatga tggcctcgtc ggccgtttcc
     4861 gcggcgagga tgtcccggcg accggatttt ccatcggcgt ctcgcggctc tacgcggcgt
     4921 tgaaggcgat caattcgccg gtcgtcgcgg gcgcgcaaca acgcggcccg gtgatcgtgc
     4981 tggttctcga tcaggccgaa ctcgcgcgct accagagttt tgtttcgatg ctacgcaatg
     5041 ccggtatcgc cgccgagctt tatctcggtt c
//
LOCUS       DASWOG010000289         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_300149,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000289 DASWOG010000000
VERSION     DASWOG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300149"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1553
                     /locus_tag="VGB93_05880"
     CDS             144..1553
                     /locus_tag="VGB93_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145670.1"
                     /translation="MSEPEHLDVLVLGSGQAGNLMAWHMARSGYRTALVERRWIGGSC
                     PNIACMPSKNEVWSARVAHLVRNAGQFGTLTTPGQIDMGQVRQRKRDMVSGEVAGFLE
                     GFKSSGAELIRGSGRFTGPKMLEVQLNDGGTRFLTADKVFLNIGTHSAMPDVPGLEAA
                     KPLTHIDILEIDYVPPHLIVLGGGYVGLEFTQAYRRFGSAVTVIDAGPQIISREDADS
                     AEAIARILSGEGIRILTGAKILKVEGSSGKAVTLTLQTGGGEERIEASDILVATGRVP
                     NTAGIGLEDTGVALDERGYIRVNERLETSTPDVWALGECAGSPQFTHVSVDDFRIVKD
                     NLAGGTRSTKDRLVPYCLFTDPPLAHVGLSERDAQRQGVSVRVAKLPTHAVLRAHTTD
                     EKLGFLKVLIDDNDHIVGFTMIGAEAGEVMAAVQTAMLAGLPYPKLRDAVIAHLTMAE
                     GLGPLLGNVPAKASVPEFA"
BASE COUNT          339 a          482 c          499 g          307 t
ORIGIN      
        1 ggcaatatgg ccgcgatcga acccgtctcg gctgaggtcg cagtaacttc atccaacacc
       61 ggcacgtatc aagacagtca gccacgatag tcagcgcccc agcgcgcatg ggactgtgcg
      121 tcgtcgcata gaaggagagc atcatgtcgg aaccggagca tctcgacgtc ttggtcctcg
      181 ggagtggaca ggcaggcaat ttgatggcct ggcacatggc gcgatcggga taccgaaccg
      241 ctttggttga acgccggtgg atcggcggct cctgtcccaa catcgcctgc atgccgagca
      301 agaacgaggt ttggagcgct cgggtcgctc atctcgtccg taacgccgga caattcggta
      361 cgctcacgac gccgggccaa atcgacatgg gccaagtccg ccagcggaaa cgcgacatgg
      421 tgagcggcga ggtcgccgga ttcttggagg gtttcaaatc gagcggcgcc gaactcatcc
      481 gcgggagcgg ccgttttact ggtcctaaaa tgcttgaggt gcagctcaac gacggcggca
      541 cacgctttct gacggccgac aaagtgtttc tcaatattgg cacgcacagc gccatgccgg
      601 atgttcccgg ccttgaggcc gcgaagcccc tcactcacat cgacattctc gaaatcgatt
      661 atgtgccgcc acatttgatt gttcttggcg gcggctatgt cggtcttgag ttcacacagg
      721 cttatcggcg gttcggcagc gcggtgacgg tcatcgacgc cgggcctcag atcatcagcc
      781 gcgaggacgc cgatagcgcc gaagccattg ctcgaattct cagcggcgaa ggcatccgga
      841 ttttaactgg cgctaaaatc ctcaaggtcg agggatcgtc cggcaaagct gtcaccctca
      901 cgctgcaaac aggcggcggt gaagaacgga tcgaagcgag cgatatcctc gtcgccacgg
      961 gccgcgtccc caacacagcc ggcatcggtc ttgaggacac aggcgtggct ttggacgagc
     1021 gcggctatat ccgcgtcaat gaacgattgg agaccagtac gcctgacgtt tgggcgctgg
     1081 gcgaatgtgc tggaagcccg caattcacgc atgtgtcggt cgacgatttt cgtatcgtca
     1141 aagacaatct ggccggcggc acgcgcagca caaaagatcg cctcgtaccc tactgtcttt
     1201 tcaccgatcc gccgttggcg catgtcggac taagcgaacg cgacgcgcag cgtcagggcg
     1261 tttccgtccg cgtggccaaa ttgccgacgc acgccgtgct gcgtgcccat acgacagatg
     1321 agaagcttgg atttctgaaa gtcctgatcg acgacaacga tcacatcgtc ggcttcacga
     1381 tgatcggcgc agaggccggt gaagtaatgg ccgccgtgca gacagcgatg ttggctggcc
     1441 tgccctatcc caagctgcgc gatgccgtga tcgcacatct gacgatggcg gaaggactcg
     1501 gcccgcttct aggcaacgtg ccggcaaaag cgtcggttcc tgagttcgcc taaatggcga
     1561 gatgcgcgtg cgcgccgttg ccagccgcaa caccgggccg cagcgctgct gaaagctgga
     1621 agaaatt
//
LOCUS       DASWOG010000290         9471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_244710,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000290 DASWOG010000000
VERSION     DASWOG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244710"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGB93_05885"
     CDS             <1..329
                     /locus_tag="VGB93_05885"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY0145671.1"
                     /translation="GIMESAASAHTIADPLLKTSANMLIVNAASVIAISGYASSRLQR
                     WALFGAATLLAGSLLFCGELSTHVFLGQRSFALAAPIGGTLMIAGWVIAAASAFASAV
                     RAPAKI"
     gene            452..826
                     /locus_tag="VGB93_05890"
     CDS             452..826
                     /locus_tag="VGB93_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0145672.1"
                     /translation="MNDTIQLQTAPRILVVEDEPFIAMLLEDMLDELGFKITASFPQV
                     AEAEKYLAGAEVDIALLDVNVGAEKIDPVADLLAARGCPFIFTTGYGQKGVPENHTDR
                     PVLQKPFQIADLANALQSQLGR"
     gene            complement(866..1861)
                     /locus_tag="VGB93_05895"
     CDS             complement(866..1861)
                     /locus_tag="VGB93_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0145673.1"
                     /translation="MNAKFKWLALRTTTLTFAAALALTFSLAEVPAQIVGNVGAVSPT
                     SLATPLGEKTRNLILGSRVIANELIQTSGERSAKITFLDRTTMSIGHNSTLVIDKYVY
                     DPTTGKGVLTATMAQGVLRFIGGEISHSSGVKVLTPVATIGVRGGMMTVSVGAEGVSV
                     MSHYGHIDVANGRNHQLLLRSGFTVHVSSHDDYIVAPGRTSLEALDKEYKTLSSRHSN
                     HHESSLTDSNAAQYGLADAPLPNDPDSMPGLDTLGAMNLGQDFVGNRSQQQQWNGANA
                     QLHAALPPAPPPVTPPPITPPPPPPPPPPTGGQPGGGRGNGGGWWGQGGTVLPRR"
     gene            complement(1871..3184)
                     /locus_tag="VGB93_05900"
     CDS             complement(1871..3184)
                     /locus_tag="VGB93_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145674.1"
                     /translation="MIGLCAGLGFAAQADGQPDRHTLAVAQARLLAAVLREPKNFDLT
                     FEYVRVSSALGDYEGAIGALERVLAFAPNLARADFELGTLYFRLGSYDNAIHYFKAAA
                     AAPDLDPKLRARLTVYLPEVEKQLSPVSWSGFLQTGLGYQSNVAALPNSGFVSIFGQN
                     APQGSGVAQKSDGEAFGLAKISNIYDFENQRGDTIETNFIGYGTGQFSLHQFDLAYME
                     ASIGPRLALDPVNWPGVTIKPYAIGNVSWVGGTSYLNSGGAGVSLGIDTARDWSVAPD
                     FEWRHISVHNPGAAQITALGTGDFLSFSIAGTYRFTDLISVQVQPIYSRANAFNAWQS
                     FNQGGVTAGLNVEFNPPFPLIPLQWTVMPYVSALWDRFDAPDPAVNASLRRCDFAYYG
                     GVMLDMPVTASFGVSGTVQYARTNSNLQNFTNDDVTILFGPTARF"
     gene            3661..5544
                     /locus_tag="VGB93_05905"
     CDS             3661..5544
                     /locus_tag="VGB93_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY0145675.1"
                     /translation="MIEIVSRARARHWPRKGSGKLALAGAAAALVIAAALLITQQVLP
                     QNLLRQQIVAQIQRSTGFSVQINGPARLRFFLQPRIIIQSLRVADRSGAIDLEAPEVV
                     GYLRLLPLLVGRFEIGHAVLYRPKLKVDFGRAPVSEGGVVQQALRASSTDNVRLQAPL
                     GKISVVEGEAFIKTKNIPNGISIDTINMQANWPRLSASADFNGQVNFRGAPVRLQGWL
                     ETPLDILRGGESAAILQIRSPILNLWSSGRFAGGTRLQYNGSVSATAPSLRKLAETIG
                     YSFSKHGTFANLDVSCDVDFENGDAAFTNLALHLDGNDYEGNLEIRDVAHTPHFSGTL
                     ASDFLDVTPFLIGQPDTALPDGLGDGTPFDLGPLNLADLDLRVSAARLRLYDLEVENA
                     ALSLLTKPGLIDLALAEATSNNGLIKGRFALSEKDQIFSLHVSGSGNGVDVQPMVFGG
                     QRPLSGSLNALVELDSSGANVDALARSLSGQAAVSANNGTIAGIDLVPTLAGADSRKP
                     GDRIAVVKGTTSVDHLTFDVTLAHGVATFNSGKITGANLQIDTMGTIDVGHRQLDISS
                     LAQITETAGKRAQTSPLWFELKGAWTNPGLYRRAAVDLPLKPAHPNDVLPQMTTSPPP
                     AEE"
     gene            complement(5545..6234)
                     /locus_tag="VGB93_05910"
     CDS             complement(5545..6234)
                     /locus_tag="VGB93_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0145676.1"
                     /translation="MQSLARVPFFKDLTDLDFERFDRRCTWRRYDEGEVVIDYEDETS
                     DVYFIIAGEVRILIRTQAGKEVILAEMRAGQFFGELSAIDSTKRSANVTALTRAELCI
                     MPAAVFREVIFASPTVCDRVLHLLTTRVRELNARLTEHSIFDLKHRLYSELLRMAGPR
                     PGKPGERSVTPPPFHHVLAARIGCRREQVTRELSTMAQEGLLEKTRGALVLSKPQVLE
                     ARLAEALREDH"
     gene            complement(6342..7295)
                     /gene="folD"
                     /locus_tag="VGB93_05915"
     CDS             complement(6342..7295)
                     /gene="folD"
                     /locus_tag="VGB93_05915"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY0145677.1"
                     /translation="MALATDAAAFQTDFLRPSGEKALLIDGKIASEIVLAEVAAATAA
                     RGTKIQPGLAVVLVGENPASQVYVRSKGKAAKTVGFHSIQHDLPDTTSEEELLVLIDH
                     LNADPKIHGILIQLPLPPSINATRVLERVSPLKDVDGFHPFNAGLLSIGDLERALVPC
                     TPAGSMVLLERAAKALGVSIAGADAVVVGRSNIVGKPMAQLLLSQNATVTIAHSRSRD
                     LPAIVGRADIVVAAVGRPEMVLGEWIKPGAIVIDVGINRVPGEGVDAQGKPKSRLVGD
                     VAFKSALSRAGAITPVPGGVGPMTIAMLMSNTLRAANLAAD"
     gene            complement(7373..8239)
                     /locus_tag="VGB93_05920"
     CDS             complement(7373..8239)
                     /locus_tag="VGB93_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY0145678.1"
                     /translation="MVRIKSGTTSVSISPQGAEIMAWSVEGRPLLWEARPDVWPETAP
                     LLFPVVGWTRNGEARVDGQTYPLALHGFARHRVFRLTAQETDRVQFELTSDAATRALY
                     PFDFRFTADYAAGAGSLSTAVTVANTSDRAMPYACGLHPGFRWPFAGGQPEDYRLVFA
                     GPENPVVPKIAPGGLIARETRRLPMERGVLPLSAALFSNDALCFLDAVSRSVRFEAPN
                     GAAIEVESEDFSHFGVWCRPGNGYLCIEQWTGFGDPEDFTGDLFAKPSMRVLPPGATA
                     RHAAHYRYKAAP"
     gene            8415..8792
                     /locus_tag="VGB93_05925"
     CDS             8415..8792
                     /locus_tag="VGB93_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY0145679.1"
                     /translation="MRTWLLTGATMVLATAAALAMVSSPPKPLGEWKIADGTANVSIR
                     NCGGNLCGYVSWAKESAAPLGREVLIDMKPNGHSWFGTAVNVVNGEQYTAHMSLEGAA
                     TLKVEGCIIGGMICGGQEWSRVR"
     gene            8929..>9471
                     /locus_tag="VGB93_05930"
     CDS             8929..>9471
                     /locus_tag="VGB93_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0145680.1"
                     /translation="MPSRLGWFGRRSLFGKYVITFVALVISILALSGATEMWFTYSDR
                     KAELLRTQMEKADDAAQRVEQFIAEIERQITWATRASSMTLDQRRIDYAMILQHTPAV
                     SEIFELDTKGHEILRLGRAGLFRNAYRDLSKSPAFLHATGPNAWLSPVMFTHHGPTIQ
                     ISVALSGKQPGVAVATVELKF"
BASE COUNT         1927 a         2863 c         2789 g         1892 t
ORIGIN      
        1 caggaatcat ggaatcggcg gcgagtgcgc atacgatcgc cgacccgctg ctgaaaacat
       61 ccgcgaatat gctcatcgtc aatgccgcat cggtgattgc catcagcggc tatgcatcca
      121 gccgattgca gcgttgggcc ctgtttggcg ccgccacgct cctcgccggg agccttctgt
      181 tttgcggcga actcagcacg cacgtttttc tggggcaaag gtcttttgct ttggcggccc
      241 ccatcggcgg cacgttgatg attgccggct gggtgatcgc tgcggcaagt gccttcgcca
      301 gtgccgtccg agcgcctgcc aaaatctagt cgcctgccaa ggcccagttt gccattacat
      361 tggccgatgc acgtctcgaa ttgacgcagg tcggccaatt tcccaaagcg ccctgctcgc
      421 atagcaatcg tagccgcgct ataggtccgg gatgaacgac acgatccaat tgcaaacggc
      481 cccgcgaatt ctcgttgtcg aagacgaacc tttcatcgcg atgctgctgg aggacatgct
      541 ggacgagctg ggcttcaaga tcaccgcgtc attcccgcag gtcgccgagg ctgagaaata
      601 tctcgcgggc gcggaggtcg atatcgctct gctcgacgtc aatgtcggcg cagagaagat
      661 cgatcccgtc gccgacctct tagccgcgcg gggctgcccc ttcatcttca cgacaggtta
      721 tgggcaaaag ggcgttccgg aaaaccatac tgatcgcccg gttctgcaaa agcccttcca
      781 gatcgccgat ctggccaacg cgcttcagag ccagctcggc cgctagcgaa ttaacgtcta
      841 aatgccgtcg gtccgcagtc cgcagctacc gtcgtggcag caccgtgccg ccctggcccc
      901 accagcctcc accatttccc cggccgccac ctggttgccc gcccgtgggg ggtggcggcg
      961 gcggtggagg tgggggagta attggtggcg gagtaactgg cggaggcgct ggtggcagcg
     1021 ccgcatgaag ctgcgcgttt gcgccgttcc attgttgctg ctgcgaccgg ttgccgacaa
     1081 aatcctgacc aaggttcatc gcacccaatg tgtcgagacc gggcatcgaa tccgggtcgt
     1141 tgggcagcgg tgcgtcagcc aacccgtatt gcgctgcgtt tgagtccgtc aacgaacttt
     1201 catgatgatt gctgtggcgg ctcgacaggg tcttatattc tttgtcgagc gcctccagag
     1261 acgtgcggcc aggcgcgacg atataatcgt cgtggctgct gacatgcact gtaaagcccg
     1321 agcgtagcag aagctgatga ttgcggccat tggcgacgtc gatatggccg taatgcgaca
     1381 tgaccgacac gccctcggcg ccgacactta cggtcatcat gccgccgcgc acgccgatcg
     1441 tggcgacggg cgtcagcacc ttcacgccgg agctgtggct gatctcgccg ccgatgaagc
     1501 gtaacacgcc ctgggccatc gtcgccgtca gcacgccctt cccggtcgtt ggatcataga
     1561 catatttgtc gatgaccaag gtcgaattgt gtccgatcga catggtggtg cgatcgagga
     1621 acgttatctt cgccgatcgc tcgcccgatg tctgaatgag ctcgttggcg atgacgcgtg
     1681 agccaaggat gaggttgcga gtcttctcgc cgagcggcgt ggcaagcgag gtcggcgaga
     1741 cggcaccgac gttgccgaca atttgcgccg ggacttcagc aagcgaaaac gtcagcgcca
     1801 gcgccgcagc aaacgtcagc gttgttgtgc gcaaagccag ccatttgaat ttcgcgttca
     1861 tcttctcgcc tcagaaacgc gccgtcggcc cgaataagat cgtcacgtcg tcattggtga
     1921 aattctgcaa gttcgagttc gtgcgcgcat attggaccgt tccggacacg ccgaaactcg
     1981 ccgtgacagg catatcgagc atgacaccgc cataataggc gaaatcgcac cgacgaaggc
     2041 tggcattgac ggcagggtcc ggcgcatcga atctgtccca tagtgcgctg acatagggca
     2101 tgaccgtcca ttgcagcggg atcagcggga atggcgggtt gaattcgaca tttagcccag
     2161 ccgtcacgcc accctggttg aagctttgcc aggcgttgaa ggcatttgcg cgcgaataaa
     2221 ttggctgcac ttgcacggag atgagatcgg tgaagcggta agttccggca attgagaagg
     2281 aaagaaaatc gcccgtaccc agcgcggtga tctgagcggc cccgggattg tgaaccgaga
     2341 tatggcgcca ctcgaaatcc ggcgcgaccg accagtcccg cgccgtatcg atgccgagcg
     2401 aaacgccggc gccgcccgaa ttcaggtagg acgtgccgcc cacccaggag acattgccga
     2461 tcgcgtaagg cttgatcgtc acgcccggcc agttgacggg atcgagcgcc agccgcggtc
     2521 caatgctcgc ttccatatag gcgagatcga attgatgtag cgaaaactgc ccggtgccgt
     2581 agccgatgaa attggtttcg atcgtgtcgc cgcgctgatt ttcgaaatcg taaatgttgc
     2641 tgatcttggc gagaccgaag gcctcgccgt cgctcttttg cgcgacgcct gaaccttgcg
     2701 gcgcgttctg gccgaagatg ctgacgaatc ccgaattcgg cagcgctgcg acattcgact
     2761 gatagccgag gcccgtctgt aaaaagccgc tccagctcac gggcgagagc tgcttttcaa
     2821 cctcgggcag atagacggtc agccgcgcgc gcaatttcgg atcaagatcc ggcgccgctg
     2881 ctgcggcttt gaaataatga atggcgttgt cgtaggagcc gagccggaaa tacagcgtgc
     2941 caagttcgaa atccgcccgc gccaaatttg gcgcaaacgc gagcacgcgc tccaatgcgc
     3001 cgatcgcgcc ttcataatca ccgagcgctg acgagacccg tacatattca aacgtcaggt
     3061 cgaaattctt tggttcacgc agcaccgcgg caagcaaccg cgcctgggcc acggcgagcg
     3121 tatgacggtc gggctggccg tcagcctgcg cggcgaagcc gaggcccgca cacaaaccaa
     3181 tcaccgccag acccgtaagg acccttcgca cagcagtgcc cccaacgctt acttccgtcc
     3241 gaatccagcc gattggactt tttatacctg tgcaataact ctaaagggct gcggggctcc
     3301 agcaagggaa attgcccgca aaggttggtc ccgctggcgt aatctcgcgg gctgtggcca
     3361 ccatgcatct gttttataaa ttgttgaatt tcaatgggtt aaatcgatat ggacttggcc
     3421 tcccaaactc gaaagcgagt ctgaaattat caatctacgc gcgtattctg aatgagcgcc
     3481 gcagaaattt ggtggatcga cgattacgac cgatgatcat aaagatgatg gattcgaggc
     3541 gcttaagagt aaacgtcgaa ttatagcccg aagcatctac gctgaacgcg ttttgcccta
     3601 tcctgagcgt cccgagacta ttctgcgcag cttcgctgat ttatgagccc cagttcgtcc
     3661 atgatcgaaa tcgtttcgcg cgcgcgggca aggcactggc cgcgtaaagg ctcggggaag
     3721 cttgcgctcg ccggtgcagc agcggcgctg gtcatcgccg cggccttgct gatcacgcag
     3781 caagtgctgc cgcaaaatct cttgcgccag caaattgttg cgcaaattca gcgttcgacc
     3841 ggcttttcgg tgcagatcaa tgggcccgct cggcttcgct ttttcctgca gccgcggatc
     3901 atcatccaat cccttcgcgt ggccgatcga tccggggcca tagaccttga agcgcctgag
     3961 gtcgtgggct acctccgatt attgcccctc ctcgtcggcc gctttgagat cggccacgct
     4021 gtgctttatc gaccgaagct caaagtcgat ttcggccggg ctccggtgag cgaaggtggc
     4081 gtcgttcagc aggcgctccg cgcgagcagc acagacaatg tgcgtttgca ggccccgctc
     4141 ggcaagattt ctgtcgtcga gggcgaggcc ttcatcaaga ccaaaaacat cccaaacggg
     4201 atttccatcg atacaatcaa catgcaagcc aattggccgc gcctgagcgc ttcggcggat
     4261 tttaacgggc aggtcaattt ccgtggcgcc cctgtgcgtc tgcaaggctg gctcgaaacg
     4321 ccgctcgata tcctgcgcgg cggtgaatcc gctgcaatcc ttcaaatcag atcaccgatc
     4381 ttgaacttgt ggtcatcggg acgcttcgcc ggcggaacga ggttgcaata taacggctcg
     4441 gtttccgcaa cggcgccctc gctacgtaaa ttggccgaga cgattggtta ttcgttctcg
     4501 aaacatggaa cattcgccaa tcttgacgtc tcgtgcgacg tcgattttga aaatggcgac
     4561 gcggctttca ccaatctcgc gctgcatctc gacggcaacg attacgaagg aaatctcgaa
     4621 atacgcgatg tcgcccatac accgcatttt tcgggcacgc tggcgagcga ttttctcgac
     4681 gtcacgccct ttttgatcgg acagcctgac accgcactgc ccgacgggct gggcgatggc
     4741 acgccgtttg acctcgggcc gctcaatctc gccgatctcg accttcgggt gtccgcggca
     4801 cggctgcggc tctatgatct ggaggttgag aacgcggccc tttcgctttt aaccaagccg
     4861 gggctcatcg accttgcgct tgccgaagcg acatccaaca acggcctgat caaaggccgc
     4921 tttgcgctct ccgaaaagga ccagattttc agtttgcacg tcagcggcag cggcaatgga
     4981 gtcgacgttc agcctatggt tttcggtggc caacggcccc tatcgggatc gctgaatgct
     5041 ctggtcgagc ttgatagttc aggcgctaat gttgacgcac ttgcccggtc gctcagcggt
     5101 caggcggctg tttcagcgaa taatggcacc atcgcgggaa ttgatcttgt cccgacgctc
     5161 gcgggcgcgg atagccgcaa acctggcgac agaatcgccg tcgtgaaggg cacaacgagc
     5221 gtcgatcatc tcacatttga tgtgacgctc gcgcacggcg tcgcgacgtt caatagcgga
     5281 aagatcactg gcgccaatct gcagatcgat acaatgggca cgatcgatgt gggtcatcgc
     5341 cagctcgata tttcatcact tgcccaaatc accgagacgg ccggcaaacg ggcgcagacg
     5401 tcgcctctgt ggttcgagct taagggggct tggacaaatc ctggacttta tcgccgggct
     5461 gcggtcgatt tgcccctgaa gccagcgcac ccgaacgatg tactccccca aatgacgact
     5521 tccccgccgc ccgcggagga atgatcagtg gtcttcgcgc aaggcctcgg caagccgtgc
     5581 ttccaaaact tgcggcttgg aaagaaccaa agcgccgcgc gtcttttcga gcaagccctc
     5641 ttgcgccatg gtcgaaagtt cgcgcgtcac ctgctcgcgc cggcagccga tccgcgccgc
     5701 aagcacatga tggaacggcg gcggcgtgac tgagcgctct cccggcttgc caggtcttgg
     5761 accggccatg cgcaagagtt cggaataaag ccgatgcttc aaatcgaaga tcgaatgttc
     5821 ggtgagccgg gcgttcagct cgcgcacgcg cgtcgtcaga agatgcaaga cgcgatcgca
     5881 gaccgtcggc gacgcgaaaa tgacttcacg aaaaaccgcc gccggcatga tgcaaagttc
     5941 ggcgcgcgtc aaagccgtga cattggccga ccgcttcgtg ctgtcgatgg ccgaaagctc
     6001 gccaaagaat tggccggcgc gcatttccgc gaggatcact tccttgccgg cctgcgtgcg
     6061 gatcaggatt cgcacctccc cagcgatgat gaaataaacg tcggaggttt cgtcctcata
     6121 atcgatgacg acttcacctt catcgtatcg ccgccaagtg cagcggcgat cgaagcgttc
     6181 gaagtccaga tccgttaaat ctttaaaaaa aggaacgcgg gcaaggctct gcattatctg
     6241 ggtccccgac gccgaccaaa acagtcggca ccatctttaa tccgcacgga ctttggcgat
     6301 agctctgcaa cgccaaagcc cacggatttc gcagcttaac cttagtccgc agccaggttg
     6361 gccgcccgca gggtgttcga cataagcatc gcgatggtca tcggcccgac cccgcccgga
     6421 accggagtga tggcccctgc ccgcgacagc gccgatttaa aggcaacatc gccaacgaga
     6481 cgcgatttgg gcttgccctg cgcatcgacc ccttcaccgg gaacacggtt gatccccacg
     6541 tcgatgacga tggcaccagg cttgatccat tcgccgagca ccatttccgg ccggccgacc
     6601 gcggcaacga cgatatcggc acgcccgacg atcgccggca aatcacggct gcgcgaatgg
     6661 gcgatggtca cggtcgcatt ttgcgagagc agcaattgcg ccatcggttt gcccacgatg
     6721 ttggagcggc caacgacgac ggcgtccgcg ccggcgatgc tgacgccaag cgcctttgcc
     6781 gcccgttcaa ggagaaccat cgagcccgcc ggcgtgcatg gcacgagcgc gcgctcaagg
     6841 tcgccgatcg agagaagacc ggcattgaac ggatgaaacc cgtccacgtc cttgagcggc
     6901 gacacgcgtt cgagcacgcg cgtcgcattg atcgaaggcg gcagcggcag ttggatcagg
     6961 atgccgtgga ttttcggatc ggcgttcagg tgatcgatga gcaccagcaa ttcttcttcg
     7021 ctggtcgtgt cggggagatc atgctggatg gaatggaagc cgacggtttt ggcggccttg
     7081 cccttggagc ggacatagac ctggctggcc ggattttcgc cgacgaggac aaccgcgaga
     7141 ccgggctgaa tcttcgtgcc ccgcgccgcg gtcgccgcgg cgacctccgc cagcacgatt
     7201 tccgaggcaa tcttcccgtc tatcagcagc gccttctcgc ccgaaggccg caagaaatcc
     7261 gtctgaaaag ccgccgcgtc cgtcgccaaa gccattttaa agcgtctccc tgaatagaat
     7321 ttccgcttat ctgtcaggtt tctgcacggc tgccaagcta gacgaacagc gatcagggtg
     7381 cggctttata gcgataatgg gccgcatggc gggcagtcgc gccgggcgga aggacgcgca
     7441 tggagggttt ggcgaaaaga tcgccggtga aatcctcagg gtcgccgaac cccgtccatt
     7501 gctcgataca gaggtagccg ttccccggcc ggcaccagac gccgaaatgc gaaaagtcct
     7561 cgctttcaac ctcgatcgcc gcaccgttgg gcgcctcgaa ccggacgctc cggctcaccg
     7621 catcgaggaa gcagagcgcg tcgttcgaga agagcgctgc gctgagcggc agcactccgc
     7681 gctccatggg cagccgccga gtctcgcggg caatgaggcc gcccggtgcg attttcggca
     7741 ccacgggatt ttcgggcccg gcaaagacga gccggtaatc ctcaggctgc cctcctgcga
     7801 agggccagcg gaaaccggga tgcaaaccgc atgcataggg catcgcccgg tcgctagtat
     7861 tggcgactgt aactgccgtg gagagcgacc cagccccggc ggcgtaatcc gccgtgaagc
     7921 ggaaatcgaa cggataaagc gcccgcgttg cggcgtcgct cgtcagttcg aattgaaccc
     7981 gatcggtctc ctgcgcagtc aagcgaaaca ctcgatggcg ggcaaacccg tgaagcgcga
     8041 gcggataggt ttgcccgtcg acacgcgcct cgccgttgcg cgtccaaccg acgaccggaa
     8101 aaagtaacgg cgccgtctct ggccagacgt caggtctggc ctcccaaagt aaaggacgcc
     8161 cttcgacact ccaggccata atctcggcgc cttgcgggct gatgctgacg ctggttgttc
     8221 cactcttgat acggaccatt ttggaatccc attgatatca gagagctgaa gggcgtccga
     8281 tagactaggc caatgtgcgc tagcgcacct tggagactta acgtcccggt ttcgacgacg
     8341 caaatcgaga agatttatag tagaattttc aacgatttgt tcttcaactc ctcggctaca
     8401 tggagatgtt cgtcatgaga acttggttgc tgacgggggc gacaatggtt ctggcgacag
     8461 cggcggcctt ggctatggtt tcctcacccc cgaagccgct cggcgagtgg aaaatcgctg
     8521 atgggactgc caatgtctcg atccgcaact gcgggggtaa cctttgtggt tatgtctcct
     8581 gggccaagga gagtgcggcg cccctcggcc gcgaagttct gattgatatg aagccgaatg
     8641 gccattcctg gttcggcacc gctgtcaatg tcgtcaacgg cgagcaatat accgcccaca
     8701 tgtcgctcga aggcgccgcc actctcaagg tcgagggatg cattatcggc ggcatgatct
     8761 gcggcgggca ggaatggtcg cgcgttcgct aaacaaaaag accttcgcga cattcagcgt
     8821 attggcgatt agaccggggc attctccctc cggctcttca tctgcgccgc cctttatgcc
     8881 agacttggcc cctgcgcatt gctgtgccgc gctgtgaagg gagcgagctt gccgtcgcgt
     8941 ttgggatggt ttggccgaag aagcctcttc ggcaaatatg tcatcacctt cgtcgctttg
     9001 gtcatttcca tcctggccct cagcggcgcc accgaaatgt ggttcaccta cagcgaccgc
     9061 aaagccgaac tcctgcgcac gcagatggag aaggccgacg acgcggcgca acgcgtcgaa
     9121 caattcattg ccgagatcga gcgccagatc acctgggcga cccgtgcgag ttcgatgaca
     9181 ctcgatcagc gccgcatcga ttacgccatg atcctgcaac acacgcccgc ggtatctgag
     9241 attttcgaac tcgataccaa gggccatgaa atcttgcggc tcggccgcgc cgggcttttc
     9301 cgcaatgcat accgggattt atcgaaatcg ccggctttct tgcacgcgac cggcccgaat
     9361 gcctggttga gcccggtcat gtttacccac catggcccaa cgattcagat ttccgtcgcg
     9421 ttgtcgggga agcagccggg cgtcgccgtc gcgacggtcg agttgaaatt c
//
LOCUS       DASWOG010000291         6197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_354565,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000291 DASWOG010000000
VERSION     DASWOG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354565"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="ftsA"
                     /locus_tag="VGB93_05935"
     CDS             complement(<1..945)
                     /gene="ftsA"
                     /locus_tag="VGB93_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0145681.1"
                     /translation="MSLPPRMRALSARKTAILSALDIGTSKVVCLIARLDPIEAPEAL
                     RGRTHRCRILGIGHQRSAGLKGGVIVDLEAAEGAIRRAVDAAERMAGVQVESVIVNIT
                     GGRIGSQHYQASVRIGDRAVTESDVHRVLEACATRTMAEGNAVLHSLPTGFSLGDMRN
                     VRDPKGMMSDELGADMHVVSGDATAVRNLMLAVERCHLAVEAVVATPYAAGLAALVDD
                     EVELGTTVIDMGGGTTSVGVFFGGGLVHVDALALGGNHVTMDIARGLNTRLADAERLK
                     TLYGACIASVSDERETISVTLAGDDGDHPTHVPKSQLVR"
     gene            complement(942..1922)
                     /locus_tag="VGB93_05940"
     CDS             complement(942..1922)
                     /locus_tag="VGB93_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY0145682.1"
                     /translation="MDGGRLLLRPVREAFEGFQPSAPVHAGVVHIAPTLVFSADYGRR
                     ASSRRVSSSANRVLALFIVPGVGTLVALLLFIGVGLYGAWLGGEYSTFVAAEGSVPDY
                     VARVAGFAIRTITISGAHALTEQEVITASDIGLKNSLPFLDVQDVRARLKMLPMVKDA
                     SITKLFPGHVVIDVEERKPYALWQMDGAVKIVAMDGTAIDTYDDQRFANLPLVVGAGA
                     NARLDEYVALLDAAGDLRARIKAGILVAQRRWTLQIDNQVEVALPEIGAAAAIAELAR
                     LQRKDHILDKDILSVDLRIPGRMFIRLSENAAAERAAALAQKSKSKGART"
     gene            complement(1889..2812)
                     /locus_tag="VGB93_05945"
     CDS             complement(1889..2812)
                     /locus_tag="VGB93_05945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0145683.1"
                     /translation="MKKHVAVLMGGISAERDVSLNSGAACAKALAEQGFQVTEVDVDR
                     NIGQVLADLRPDVAFNALHGPFGEDGIIQGVLEMLGIPYTHSGVLASALAMRKDRAKD
                     VLKAAGLPVAESRTVDRLTAAKTHVLPPPYVVKPLAEGSSFGVLVVKEDQLHPPQELT
                     REDWLYGEEVLVERYVAGRELTCAVIDGKAYDVIEIKASDGGWYDYKAKYAAGGSVHE
                     LPANLKENIYHFVQESTLKAHKALGCRGVSRADFRYDDRPGGTGEIVILEVNTQPGMT
                     ATSLVPEIAAYAGLTFGELVKWMVEDSSCDR"
     gene            complement(2906..3703)
                     /gene="modA"
                     /locus_tag="VGB93_05950"
     CDS             complement(2906..3703)
                     /gene="modA"
                     /locus_tag="VGB93_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0145684.1"
                     /translation="MSISIRRIMAFCAIGVSILGTGHEASAQANGKVVVFAAASLATA
                     LNAIQPIFAKDTGKPVIFHYGSSGVLAKQKEQGAPADVFISADTKWIDYLQKKNLLAK
                     GTRRDLLGNDLVLVEPAGSDTSLKIKAGFDLAKATGDGKIAVCTIGSCPAGIYAKDAF
                     TKLGIWSAVEPKLAQANNVRDALALVARGEVKFGVVYKTDAKAEPKVRVVDIFSADSH
                     APIVYPVAIVGSSKNPDAATFEAFLSSQAAVKILTGQGFKFLSKPSE"
     gene            3875..4321
                     /locus_tag="VGB93_05955"
     CDS             3875..4321
                     /locus_tag="VGB93_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145685.1"
                     /translation="MPSPSDFRAADAPLIEAVKVDDARARQGEVTYRVLEPRHEPKQK
                     NADKRAEGRHRTRLHSGKILDLTNKFLIDCQIHNRSAHGARLILAAKVKAPRRFRLFG
                     DTDGELVDAKIIWQRGQNVGVTFLEEKPEALTLAQITALRKKLYVV"
     gene            complement(4329..4892)
                     /locus_tag="VGB93_05960"
     CDS             complement(4329..4892)
                     /locus_tag="VGB93_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145686.1"
                     /translation="MASSIRNKSAKIADKGDGATAAPPLLQQAAARLGWGSIVDGLLG
                     GVGGATAVVCASFAIYMSAHPRPAFNGLEHLMIFAQPNYQDPASIAETAPPADRKGID
                     YIATGTIHAARASAPPPSIDFDVPLVSNYRLHYVRDGEALIVGKDDAVYRVGLGTMVP
                     GVGRVIAIERRGDQWVVVTPRGLIVAR"
     gene            complement(4918..5298)
                     /locus_tag="VGB93_05965"
     CDS             complement(4918..5298)
                     /locus_tag="VGB93_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145687.1"
                     /translation="MVVAFGWALLLSGAPTLQIFAAAIGHKLPFDMPVIAQATILSGF
                     GLAIVGALQTGFSALKHFFDTVLERTSRPGEPVNKKPVTKVVERGRLKDRPYVLFGDG
                     SIEVETLLGQRRFASFKDAREFIG"
     gene            complement(5385..5834)
                     /locus_tag="VGB93_05970"
     CDS             complement(5385..5834)
                     /locus_tag="VGB93_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145688.1"
                     /translation="MIKLIASCVWICLLTSAAAYAAIFVTGFHAVAPPPKEQSSPAME
                     YKKLPAISIPMIADGAVQGYVVAELGYTYDQNAKASIPPDAYLLDETFRKVYSDTTLD
                     FRHLEKYDVNGMTKDLARRVNERLHAAVVKDVLVQAMNFVPKSDIPK"
     gene            complement(5831..>6197)
                     /locus_tag="VGB93_05975"
     CDS             complement(5831..>6197)
                     /locus_tag="VGB93_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145689.1"
                     /translation="RLQALREAFSQTVERIERLVDLETATHEQYRPIDFSEFNHRKSH
                     AVLELSRSIRALGSEAHDDSARAKLEGLSGKLERNLATLEIHLKAVRQVCAVISRAIQ
                     SEESDGTYSSSIHRRYEQS"
BASE COUNT         1328 a         1892 c         1758 g         1219 t
ORIGIN      
        1 tctcaccagt tgcgattttg gcacatgagt cggatggtcg ccgtcgtcac cggccaatgt
       61 cactgaaatc gtctcgcgtt cgtcagagac cgaagcaatg cacgcaccgt aaagcgtctt
      121 gagacgttcg gcgtccgcaa gcctcgtgtt caagccgcgt gcaatatcca tggtaacatg
      181 attgccgcca agcgccagtg catcgacatg gacgagtccg ccgccgaaaa agactccgac
      241 cgaggtcgta ccgccgccca tatcgatgac ggtcgtgccg agttcaactt cgtcgtcgac
      301 caacgcggcg agaccggcgg catagggtgt ggcaacgacc gcctcaaccg caaggtggca
      361 gcgctcgacc gccagcatca gattgcggac cgccgtggcg tcaccgctga cgacatgcat
      421 gtcggcgccg agttcgtcgc tcatcatccc cttcggatcg cgaacattgc gcatatctcc
      481 aagggagaag ccggtcggca atgaatgaag gacggcattg ccttctgcca tcgtgcgcgt
      541 ggcgcaggct tcgagcacgc gatgcacatc ggattctgtg acagcgcggt cgccgatgcg
      601 aaccgaagcc tgataatgct gcgaaccaat ccggccgccg gtaatgttga cgatgacgct
      661 ctcgacctga acgccggcca tgcgttccgc cgcatcgacc gcacgccgga tcgcgccttc
      721 cgcggcctca aggtcgacga tcacgccgcc cttcaagccc gctgaacgct gatggccgat
      781 gccaaggatc cggcagcgat gcgtgcgccc gcgcaaggcc tcgggtgctt cgataggatc
      841 gagacgcgcg atcaggcaca cgacttttga tgtgccaata tcgagcgccg acaggatggc
      901 agttttacgc gcagaaagcg cccgcatacg cggcggcagg ctcatgtccg cgcgcctttc
      961 gatttggatt tctgcgccag agcggcggcg cgttcagccg cggcgttttc cgacaatcgg
     1021 ataaacatcc ggcccggaat acgcaggtcg accgacaaga tgtccttgtc gaggatgtga
     1081 tccttgcgct gcaatcgggc aagttcggca atcgccgcgg cggcgccgat ttccggcagc
     1141 gcgacttcga cctggttgtc gatctgcaat gtccagcgcc gctgcgcgac gaggattcca
     1201 gccttgatcc gcgcccgcag atcaccggcg gcgtcaagca gcgcgacata ttcgtcaagc
     1261 cgcgcattgg caccggcgcc gacaacgagg ggcaaattgg caaaacgctg gtcgtcgtaa
     1321 gtatctatcg ccgtgccatc catggcgacg atcttcaccg cgccgtccat ttgccagagc
     1381 gcatagggct tgcgttcctc aacatcgatg acaacgtgac cggggaagag cttggtgatg
     1441 cttgcatcct tgaccatggg cagcatcttg agccgtgcac gcacgtcctg gacgtcgagg
     1501 aacggcagcg aattcttcag gccaatgtca ctcgcggtaa taacttcctg ctcggtcaag
     1561 gcatgtgcgc cagagatcgt gatggtccga atggcgaagc cggcgacccg ggcgacataa
     1621 tccggcacgc taccttcggc cgcaacgaag gtggaatatt cgccgccaag ccatgcacca
     1681 tagagtccga cgccgataaa cagcagcaac gcgacaagcg tacccacacc cggcacaata
     1741 aaaagcgcga gaacgcggtt cgccgaagac gacacacgcc ggctagacgc acggcgaccg
     1801 taatcggcgc tgaaaaccag cgtcggcgct atatggacaa ctcccgcatg gaccggagcg
     1861 gagggttgga agccttcgaa agcctccctc accggtcgca ggaggagtct tccaccatcc
     1921 atttgacgag ctcaccgaat gtcaggccgg catacgcggc gatttcgggc acaagcgacg
     1981 tggcggtcat gccgggctgc gtgttgacct cgagaatgac gatttcgccc gtacctccgg
     2041 ggcggtcgtc ataacgaaag tcagcacggc ttacgccgcg acacccaagc gccttgtgcg
     2101 ccttgagcgt cgactcttga acaaagtggt aaatattttc tttaagattt gccggaagtt
     2161 cgtgaaccga ccccccagcg gcatatttgg ccttgtaatc gtaccagccg ccgtccgagg
     2221 ctttgatttc aatcacatcg taggctttgc cgtcgatgac cgcgcaggtc agttcgcggc
     2281 ctgcgacata acgctcaacg agcacctcct cgccataaag ccagtcctcc cgtgttaact
     2341 cttgtggcgg atggagctgg tcttccttga caacgagaac accgaatgaa gagccctcgg
     2401 ccagcggctt caccacataa ggcggcggaa gaacatgcgt cttggcggcg gtcagacgat
     2461 caacggtccg gctttcggcc accggcagac cggccgcctt caagacgtct ttggcacggt
     2521 cctttcgcat cgctagcgcc gaggcgagta cgcctgaatg ggtataggga atcccgagca
     2581 tttcgaggac gccctggatg attccgtcct ccccgaaagg gccatgcagc gcattgaaag
     2641 cgacatcagg cctgagatca gcgagaacct gcccgatatt tcgatcgacg tcgacctcgg
     2701 tgacctgaaa cccctgttcc gccagcgcct tggcgcaggc ggctcctgaa tttagcgaca
     2761 cgtctcgctc agcggaaatc ccgcccatca agacggcaac gtgctttttc attgatattt
     2821 ccgaaagtta ccgctggata ttccgctgaa agccgttccg cggctcttcc agatttaaac
     2881 gaaaaaacac tgctttaacc ataagttatt cggacggctt cgacaaaaac ttgaagccct
     2941 gcccggtcag gattttcact gcggcctgcg aactcaggaa agcttcaaac gtcgcagcgt
     3001 cggggttctt cgagcttccg acaatggcga caggatagac gatcggcgcg tggctgtcgg
     3061 cggaaaaaat atcgacaacg cgaactttcg gttcggcctt ggcgtccgtt ttgtaaacaa
     3121 cgccaaattt gacttcgccc cgcgcaacga gcgccagtgc gtcgcggaca ttgttggcct
     3181 gagccaattt cggctcgacc gcgctccaga tgccaagctt ggtgaacgca tctttggcgt
     3241 agatgccagc cgggcaggag ccgattgtgc aaacggcgat cttgccgtcg ccggtggctt
     3301 tcgcgagatc gaagcccgcc ttgatcttaa gactcgtatc gcttccggcc ggctcgacca
     3361 gaacgaggtc gttgcccaga agatcgcgcc ttgtcccttt cgcgagaaga tttttctttt
     3421 gcaggtaatc gatccatttg gtgtccgccg agatgaaaac gtcggccggc gcaccctgct
     3481 ctttctgctt tgctagcaca cccgaggagc cgtagtgaaa gatgactggc ttgcccgtgt
     3541 ccttggcaaa aatcggttgg atcgcattga gagcggtcgc caggctggcc gcggcgaaga
     3601 cgacgacctt cccgtttgct tgcgccgatg cctcgtgtcc cgtgcccagg atggagaccc
     3661 cgatcgcaca aaaagccatg atccgtcgga tggaaataga catgtcttcc ctcagaattg
     3721 cggtcggcag cacgccgaaa cctccggctt tatgcaatga ttaacgcgcc ctgtgcaata
     3781 atgaactatg cacgagccta catagctcgt gacacgacag tgctcgcggc ctgcgtctgc
     3841 cgaatgccgc atcggcgcgt attctgggta gcccatgcct tcgccatctg attttcgggc
     3901 tgccgacgcg ccgttgatcg aagccgtcaa agtggacgat gcgcgcgcaa ggcagggcga
     3961 agtgacgtat cgcgttctcg aacccaggca cgaacccaag caaaagaacg ccgataaacg
     4021 tgccgaaggg cgccaccgga cgcgcttgca ctcaggcaag atcctcgacc tcaccaataa
     4081 atttcttatc gactgccaga tccacaatcg ctccgcgcat ggcgcgcggc tcatcctggc
     4141 cgcgaaggtc aaggcgcccc ggcggtttcg ccttttcggc gataccgacg gcgaacttgt
     4201 tgacgccaag atcatttggc aacgcggcca gaatgtcggc gtgacttttc tggaagaaaa
     4261 gcccgaagcc cttacgctcg cacaaatcac cgcgctgcgt aaaaagctct acgtcgtctg
     4321 aaccaagatc agcgcgcgac gatcaacccg cgcggcgtga ccacaaccca ttggtcgccc
     4381 cgccgctcga ttgctatgac gcgccccacg cccggcacca tcgtcccaag tccgacgcgg
     4441 taaaccgcat cgtctttgcc aacgatgagc gcctcgccgt cgcgaacgta atgcagcctg
     4501 taattactga cgagaggcac atcgaaatct atggagggcg ggggcgcgct ggcgcgtgcc
     4561 gcatgaatgg tgccggtcgc tatataatcg atgccttttc gatccgccgg cggcgcggtc
     4621 tccgcaatag atgccggatc ctgataattg ggctgggcga aaatcatcag atgctcaaga
     4681 ccattgaatg cgggccgtgg atgggcactc atataaattg cgaagctcgc gcaaacgacc
     4741 gccgtggcgc caccgacgcc gccgagtaaa ccgtcaacga tcgagcccca tcccagccgc
     4801 gccgcagcct gctgcaagag aggcggcgcc gcagtcgcgc catctccctt gtccgcaatc
     4861 ttcgccgatt tgttccgtat ggaggaagcc atggtgccac cgccgatcta aggtctgtta
     4921 tccgatgaat tcacgcgcgt ccttgaacga ggcaaagcgg cgctgtccga gcagcgtctc
     4981 gacttcgatc gagccgtcgc cgaacaggac ataagggcga tctttcaagc gcccgcgctc
     5041 gacgactttg gtgacgggct ttttgttcac cggttcacca gggcgcgagg tccgctccag
     5101 cacagtgtcg aaaaaatgct tcagggcgct gaaacccgtc tgcagagcgc caacgatcgc
     5161 gagaccgaag ccgctcaaaa tcgtcgcctg ggcaatgaca ggcatatcga acggcagctt
     5221 atgtccgatc gccgccgcga agatttgcaa tgtcggcgcg cccgacaaca acagcgccca
     5281 gccgaacgcc acaaccatga atccgatcca agtcaatcga tcaaaagcca tttgctcctc
     5341 catctttcaa aatttgaatc cctctcaagc gcaccgttcg actctcactt cggtatgtcg
     5401 ctcttgggaa cgaagttcat cgcctgcacc agcacgtctt tgacgacagc agcgtggagg
     5461 cgttcattga cgcgtcgcgc cagatctttg gtcattccgt tcacgtcgta tttttcgagg
     5521 tgacggaaat cgagcgttgt gtcggaataa actttgcgga acgtctcatc gaggagataa
     5581 gcgtccggcg gaatcgatgc cttcgcgttc tgatcgtaag tgtagccgag ttcggcgacg
     5641 acatagccct gcacggcgcc gtcggcgatc attggaatag agatcgccgg caatttcttg
     5701 tattccatcg ccggagaaga ttgttctttt gggggcggtg cgactgcatg gaagccagtt
     5761 acgaaaatcg cggcgtaagc agcagccgat gtcagcaggc atatccagac gcagctggca
     5821 atcagcttta tcatgattgt tcgtacctac gatggatcga cgaagaatat gtaccgtcag
     5881 attcttccga ctggattgca cgggagatga cggcacaaac ctggcgcacg gccttaaggt
     5941 gaatttcgag cgtggcgaga ttgcgctcca gtttgccgct cagaccttca agcttcgccc
     6001 gcgcgctgtc atcgtgcgct tccgagccga gcgcacggat cgaacggctc aattccaata
     6061 ccgcgtggct cttgcggtgg ttgaactcgg aaaaatcgat gggacggtat tgctcatgcg
     6121 tcgcggtctc aagatcgacg agacgctcaa tacgctctac cgtctgcgaa aatgcctcac
     6181 gcagcgcctg aagtcgt
//
LOCUS       DASWOG010000292         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_6728, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000292 DASWOG010000000
VERSION     DASWOG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6728"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..683
                     /locus_tag="VGB93_05980"
                     /pseudo
     CDS             325..683
                     /locus_tag="VGB93_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589706.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            864..1280
                     /locus_tag="VGB93_05985"
     CDS             864..1280
                     /locus_tag="VGB93_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0145690.1"
                     /translation="MSQPVRHIAMLVGALSLVAASLAVPAFTQASAATALTAADTDKD
                     GTLDLNEVKAAAALEFDKLDKDKDGTLDLKEAAHHVSKKNFVAADPDKDKTLSKDEYI
                     ALAVTLFKAANPDGDSTIDSKELYSTAGIELDRVIR"
     gene            1387..>2454
                     /locus_tag="VGB93_05990"
     CDS             1387..>2454
                     /locus_tag="VGB93_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145691.1"
                     /translation="MREAFSSSASEPKPGQTQGPAASAVEHDKTDWAFARKEAEDARD
                     RAEAASEAKSRLLATMSHEIRTPLNGIMGFADLLAATELEPEQRAYVEAIRSSGKSLV
                     ALVNEILDLSKLEAGKITLNEAPFDVALLVEGVIEILAPRAHAKGLDIASSIGADVPA
                     TVMGDAARLRQVLLNLAGNAVKFTEHGGLGLRVTRAHDSIKFAVTDTGSGVAAEDAEL
                     IFEEFTQTTPPESSGTGLGLSISRRLAERMGGKLELETTLAHGATFALTLPLAAVDGP
                     SASTRIERRLLVVSPSPFEAPYLVEKLVELGAEALRAPTLKSGLAAIDQAVLAETPPD
                     TLIIDCVFGNEAIAQLQTAADA"
BASE COUNT          487 a          792 c          681 g          494 t
ORIGIN      
        1 ttttttcttg cccttcgcag aatccttgag cttggccacg ctgcgccgac ggagcggctg
       61 cggcacggca gtcttctgcg gaaactcgat gatgcctgat tgcctccggg catttcgtag
      121 ctccagagct ggccatcgcc cttccgagca gtcacgtgca ccgagtcgta accagtgcca
      181 agttcgttca ccttcgagca gatttcgatc ttcatcgcct caggcgcgtc ggcaaagggc
      241 attgccggat ccaggaagtt gtcgttggcg cctgtcgcaa tgttgcagcc tttgccgcta
      301 cgaactgctt ttttgaatcg cgccagcacg gatttgctgt tgcccccggc gtcagaaggc
      361 tccgcggagg tcgccgcgcg ctaactgccg cccgccaaat ccagatcaag cgctatgcgg
      421 ccctcggctt gccgcacgtt tcggcgaatg ccgctgcccc cggctcaatc atcgaagaag
      481 ctgcaaggtc ggacccagtg ggcctgtcgc tgctgcgtga ggcctcggag cggctcaatc
      541 tatccgcccg cggcttctac cgcgtcctga aactcgcgcg gacaatcgcc gatctcgagg
      601 gcgccgcgac ggtcggccgg gcccatctcg ccgaggccct ctcctatcgc atcaatggcg
      661 gccaggaatt gcgcgccgct tgaggcgctg atcttgtcgc aacgcgatat ccgtcgggaa
      721 aaattccaga ttgcctcagc ctttctcgat caatgcggga attcgggaac tgttggcact
      781 cgtttcaggt tgctcgatac ccatattttt ctatatgttc ccgtcattct cgcactgcac
      841 aataattcat ggaggttgaa gacatgtccc aacccgttcg tcacattgca atgctggtcg
      901 gtgccctcag ccttgtcgcc gcttcgttgg cggtcccggc ctttacccaa gcttcggctg
      961 cgacggctct caccgccgcc gacaccgata aggacggcac gctcgatctc aacgaggtga
     1021 aagccgccgc cgcgttggaa ttcgataagc tcgacaagga taaggacggc acgctggacc
     1081 tgaaggaagc ggcccatcac gtcagcaaga agaacttcgt cgccgccgat ccggacaagg
     1141 acaaaaccct gagcaaggac gaatatatcg ccttggccgt gaccctgttc aaagccgcca
     1201 atcccgacgg cgacagcacg atcgattcga aagaattgta ttcgaccgcc ggaatcgaac
     1261 tcgatcgcgt gatccgctaa gctaacggtt caagcggcat cggttccgct tgccgcttag
     1321 gacttcgcag caaaagcgct ctttcagggc aacacaatcg caaggcttcg cgcattacaa
     1381 ttatgtatgc gcgaagcctt ttcatcatcc gcgtccgagc ccaagccagg tcagacccaa
     1441 ggtcctgcag cgtctgctgt cgaacatgac aagacggatt gggcttttgc gcgcaaggaa
     1501 gcggaagacg cgcgcgatcg cgccgaggcc gcgagcgaag cgaaatcgcg cctgctcgcg
     1561 acgatgagcc acgaaatccg cacccctctc aacggcatta tgggcttcgc cgatcttctg
     1621 gcggcaaccg agcttgagcc cgagcagcgc gcttatgtcg aggccatccg gagttcgggc
     1681 aaaagccttg tggcactcgt caacgaaatc ctcgacctct caaaactcga agccggcaag
     1741 atcacgctca atgaagcgcc atttgatgtc gcgctgctcg tcgaaggcgt gattgagatt
     1801 ctggcgcccc gcgcgcatgc caaaggtctc gacatcgcaa gctccatagg cgccgatgtg
     1861 cctgccaccg tcatgggcga cgcggcgcgc ttgcgccagg tcctgctcaa tctcgccgga
     1921 aacgccgtca aattcaccga acacggtggc cttggcctgc gtgtgacccg cgctcacgat
     1981 tccattaaat tcgccgtcac tgacaccggt tctggcgtcg ccgcggaaga tgccgaactc
     2041 atcttcgaag aattcacgca gacgacgcca cccgaatcca gcggcacggg tctcggccta
     2101 tcaatctcgc ggcgccttgc cgagcgcatg ggcggcaagc ttgaactcga aaccacattg
     2161 gcgcatggcg caacctttgc gctgacattg ccgctcgcag ccgtagatgg gccgtctgca
     2221 tcaacgcgga tcgaacggcg gcttcttgtc gtctcaccgt cgccattcga agcgccttat
     2281 ctcgtcgaga aactggttga gcttggcgct gaggccctgc gcgcgccgac attgaaatcc
     2341 ggccttgccg ctatcgatca ggcagttctc gccgaaacgc ccccggacac gctcatcatc
     2401 gattgcgtgt tcggcaacga agctatcgca caacttcaga ccgctgcgga tgct
//
LOCUS       DASWOG010000293         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_79734,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000293 DASWOG010000000
VERSION     DASWOG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79734"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VGB93_05995"
     CDS             complement(<1..504)
                     /locus_tag="VGB93_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0145692.1"
                     /translation="MKKPQRAAAADLAKQEAMKAPGLAARLAAAAILRDVTVSGHTLD
                     EVFAAPTGLSRLGGLAPRDIPLIRSIVTAALRRLGTIRHALAALLDDGFPRTAPQLEW
                     TLTVAAAQILFLDVPDHAAVDLAVRMARLDSKCAPYAALVNGVARNLIRRREEFLAVG
                     PALDIDTP"
     gene            686..898
                     /locus_tag="VGB93_06000"
     CDS             686..898
                     /locus_tag="VGB93_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEY0145693.1"
                     /translation="MTRNQSLDEKTGDQIEPRALSAAAQRALTEAAARRVQKQEVTRP
                     AEIGGRDGPDPIRYGDWEVKGIASDF"
     gene            complement(909..>1698)
                     /gene="pheS"
                     /locus_tag="VGB93_06005"
     CDS             complement(909..>1698)
                     /gene="pheS"
                     /locus_tag="VGB93_06005"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0145694.1"
                     /translation="HTSPLDLGRVHPISQVMDELAAIFADLGFAIAEGPDIETDDYNF
                     TKLNFQPDHPAREMHDTFFLKPDASGERKLLRTHTSPVQVRTMLTQRPPIRVICPGRT
                     YRCDSDQTHTPMFHQVEGLVIDKQANLGNLKWVLSEFCKAFFEVPDVKMRFRPSYFPF
                     TEPSMEVDIQCSRKAGEIRFGEGEDWLEILGCGMVHPNVLRNCGLDPEVYQGFAWGIG
                     IDRIAMLKYGMPDLRAFFDADVRWLQHYGFRPLDIPTLLAGLSA"
BASE COUNT          339 a          501 c          576 g          282 t
ORIGIN      
        1 tggtgtgtcg atatcgagcg ccgggcccac ggcgaggaat tcctcgcgcc ggcgaatgag
       61 attgcgcgcg acgccgttga ccagcgcggc atagggcgcg catttcgaat cgagccgcgc
      121 catccggacg gcgagatcga ctgccgcgtg atcgggaacg tcgagaaaca ggatttgcgc
      181 ggccgcgacc gtcagcgtcc attcgagttg cggcgcggtg cgcggaaagc cgtcgtcgag
      241 gagggccgca agcgcgtggc gaatcgtgcc gagccggcgc agcgctgcgg tcacgatcga
      301 gcggatgagc ggaatgtcgc gcggcgcaag gccgccgaga cgggagaggc cggtcggtgc
      361 cgcgaaaacc tcgtcgagtg tatggccgct gacggtgaca tcgcgaagga tcgcggctgc
      421 ggcgaggcgc gcggcgaggc ccggcgcctt catggcctcc tgcttggcaa gatcggcggc
      481 ggcagcgcgc tgcggcttct tcatcaagag cgccccctga cagtccgcgg atcgcgggcc
      541 gaacgcgtgc tgccaggcag atcggccgac gcggatgatt cgtagagact taccattgcc
      601 ttagcttgtg acggaaatca gactttcaga taagctaccg ttgtgccagc gtaatgcggc
      661 ggaaagcgcg cctgcgcaca gtgcgatgac aaggaatcag agtttggacg aaaagaccgg
      721 ggatcaaatc gaaccgcggg cgctgagcgc ggcggcgcaa cgcgctttga ctgaggccgc
      781 ggcgcgccgc gtacaaaagc aggaagtcac acgcccggcc gaaatcggcg gccgcgatgg
      841 gcccgatccg atccgttatg gcgattggga agtgaagggc atcgccagcg atttttgagc
      901 gccgccggtc aggcgctcaa gcccgcgagc agcgtcggaa tgtcgagcgg ccggaagcca
      961 taatgctgca gccagcggac gtccgcgtcg aaaaaggcgc gtaaatccgg catcccgtat
     1021 ttgagcatgg cgatgcgatc gatgccgatg ccccaggcaa agccctgata aacttcggga
     1081 tcgaggccgc aattcctcag cacattggga tgaaccatcc cgcagccgag aatttcgagc
     1141 caatcctcgc cctcgccgaa gcgaatctcg ccggctttac gggagcactg gatatccact
     1201 tccatcgacg gctcggtgaa cggaaagtag ctcgggcgga agcgcatttt gacgtcaggc
     1261 acttcaaaga aggccttgca gaactcgctc aacacccatt tcagattgcc gagattggcc
     1321 tgcttgtcga taactaagcc ttcgacctga tgaaacatcg gcgtgtgggt ctggtcggaa
     1381 tcgcagcgat acgtccggcc ggggcagatg acgcggatcg gcggccgctg cgtcagcatg
     1441 gtgcgcacct gcaccggact cgtatgcgtg cgcagcagtt tgcgctcgcc cgacgcgtcc
     1501 ggcttcagga agaacgtatc gtgcatctcc cgcgccggat ggtccggctg aaagttgagc
     1561 ttggtgaaat tataatcgtc ggtctcgatg tccggcccct cggcgatggc gaagccaagg
     1621 tcggcgaaaa tcgccgcgag ttcgtccatc acttgcgaga tcggatgaac gcggccgaga
     1681 tcaagcgggc tcgtgtgc
//
LOCUS       DASWOG010000294         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_153077,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000294 DASWOG010000000
VERSION     DASWOG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153077"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGB93_06010"
     CDS             <1..328
                     /locus_tag="VGB93_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0145695.1"
                     /translation="RLGLALAEIGDSAGVKVSDEEVAQALYERARQYPGMEKQFVDFY
                     RNNAERLSEIRGPLFEEKVIDHVLGQAKVADRHVSKDDLVKEVEAAEKAAEEEAQKGL
                     APTPAG"
     gene            424..1080
                     /locus_tag="VGB93_06015"
     CDS             424..1080
                     /locus_tag="VGB93_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0145696.1"
                     /translation="MRDRDPVEIYDNYLIPQVIENTSRGERGFDIYSRLLRERIIFLT
                     GPVEDHMASVIVAQLLFLEAENPKKEISMYINSPGGVVTSGLAIYDTMQFIRPKVSTL
                     CIGQAASMGSLLLAGGEAGMRYALPNARIMVHQPSGGFQGQASDILRHAEDIMKVKRR
                     LNEVYVRHTGRDYDTIEATLDRDHFMSGDEAKAFGIVDQVIEKRPDETPAGKAAAAAA
                     "
     gene            1534..>1707
                     /locus_tag="VGB93_06020"
     CDS             1534..>1707
                     /locus_tag="VGB93_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEY0145697.1"
                     /translation="MTKVGGSDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSALVK"
BASE COUNT          370 a          465 c          543 g          329 t
ORIGIN      
        1 gcgtcttggc ttggccttgg ccgaaatcgg cgacagcgcc ggtgttaaag tttcggatga
       61 ggaagtcgct caggcacttt atgagcgtgc gcgccaatat cccggaatgg aaaagcaatt
      121 cgtcgatttc taccgcaaca acgccgagcg tctttccgag attcgcgggc cgctgtttga
      181 ggaaaaggtc atcgatcatg tcctcggcca ggccaaggtc gctgaccgtc atgtcagcaa
      241 ggacgacctg gtgaaagagg tcgaggcggc ggaaaaggcg gccgaagaag aggctcagaa
      301 ggggctggca ccgacgcctg cgggttaagg ctcccgcagg cgttgcgaaa gcggcggaag
      361 cgattatctt tcttagctga agcttctaag atctagccga gatggcatag aaaaggttgc
      421 acgatgcgcg atcgcgatcc tgttgaaatt tatgataact acctgattcc gcaggtcatc
      481 gagaacacga gccgcggtga gcgcggcttc gacatttatt cgcgcctcct gcgcgagcgg
      541 atcatcttcc tgaccggccc ggtcgaggac cacatggcct cggtcatcgt ggcgcaattg
      601 cttttcctcg aggcggaaaa tccgaaaaaa gagatctcga tgtacattaa ttcgccgggc
      661 ggcgtggtga cctcgggtct ggcgatctac gacaccatgc agttcatccg tccgaaggtt
      721 tcgacgctct gcatcggtca ggcggcctcg atgggctctc tgctccttgc cggtggcgag
      781 gcgggcatgc gctacgcact gcccaacgcg cgcatcatgg tgcatcagcc gtctggcggc
      841 ttccaggggc aggcgtcgga tatcctccgc cacgccgagg atatcatgaa ggtgaagcgc
      901 cggcttaatg aggtctacgt gcgccacacg ggtcgcgact atgacacgat cgaagccacg
      961 cttgaccgcg accatttcat gtccggcgac gaggccaagg ccttcgggat tgtcgaccag
     1021 gtgatcgaga agcgtccgga cgagacgccg gccggcaaag ctgcagcggc ggcggcttaa
     1081 acgctgcttc ctcgcgaggt ttcttgattt tcagggcgca tccggccgcg tgccgaagtg
     1141 cacggcagga atcgggtgcc cctggaatag aatgccgtgg ccgtggcggg cgcatgtatg
     1201 tggagaagag gcagcaaacc ttggcaactc ggccactggc acatagattg ctgcttagcg
     1261 gcacactcgc ccgcttaact taataaacgc aaattgatga cagtgtgaag tcagttggat
     1321 gcgccgatgt ggcataaaag ccgcgtccga cgcgtcaaat tggaccgggg cttgatcccg
     1381 ggcctcccgc gtgatgcact tgcgtttacc aaaaacgacg cagatgcgaa tgggcggggg
     1441 gcgtaaaagg cgggcgagag gttcgagatc gagctttcgc ccggcgcaag aaaacagggt
     1501 ccggcttcgg tctggcccat aaggagatag gccatgacga aggtcggcgg cagcgactcg
     1561 aagaacacgc tctactgctc attttgcggc aagagccagc acgaagtccg caagctgatc
     1621 gcgggtccga cggtattcat ttgcgatgaa tgcgtcgagc tctgcatgga catcatccgc
     1681 gaggaaaaca aatccgcgct ggtgaag
//
LOCUS       DASWOG010000295         8179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_42899,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000295 DASWOG010000000
VERSION     DASWOG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42899"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGB93_06025"
     CDS             complement(<1..693)
                     /locus_tag="VGB93_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY0145698.1"
                     /translation="MIASSCIARADETGISLWVPGFFGSLAATPQTPGFALANIFYAP
                     SVGAGGSVVFARQVSRGDITANFNGNLNARLDGRADLYLAIPSYTFSTPVLGAQAMIA
                     MAIPYGGSFAGVNATLNGVVGPREFTVSRGTSDNVLGFGDLLPMFSLRWNAGVDNYMA
                     YVTSNIPIGVYNPDNLVNLGIGHAATDAGGAYTYFNPQTGNELSGTLGFTYNYENPQT
                     QYQNGVDMHFDWG"
     gene            1722..2072
                     /locus_tag="VGB93_06030"
     CDS             1722..2072
                     /locus_tag="VGB93_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145699.1"
                     /translation="MLIAPDVVQARGGFHGGGFHGGGRGFHGGGGRWAGGGGHWHGGG
                     GRWAGGGGHWRGGGGYWRGGRWYGGGGWGWGAAAAGAAVGAAAVGAAAASSCYQQQNV
                     WNGYQYVPQWVRVC"
     gene            2323..3246
                     /locus_tag="VGB93_06035"
     CDS             2323..3246
                     /locus_tag="VGB93_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0145700.1"
                     /translation="MVFIPGAMFRMGSNKHYPEEAPVHRVTVDGFWIDTTPVTNRQFD
                     DFVRATGYVTFAEIAPDSKDYPGALPHMLYAGSLVFTPPSHRVDLRNWGEWWRFLKNA
                     DWRHPYGPGSGIDGLDDHPVVHIAHVDALAYAKWVGKDLPTEAEWEFAARGGLDGAEY
                     AWGDEFTPGGKYMANTWHGEFPRENLKLDGYDRTSPVKAFPPNGYGLHDMIGNVWEWT
                     NDWFAQKHRPDAQKACCIPENPRGGRADESFDPAQPKIKIPRKAIKGGSHLCAPNYCR
                     RYRPAARHAEAVDTSTSHLGFRCVFRKGAAL"
     gene            3243..4994
                     /locus_tag="VGB93_06040"
     CDS             3243..4994
                     /locus_tag="VGB93_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY0145701.1"
                     /translation="MNSSDNEADKTQKPREGVNRRSVLLSGTALAAASAIASGAPQVA
                     EAQQPPASGGKQPNILVIMGDDVGWFNIGAYHQGIMSGKTPNLDKLAADGMRFTDYYA
                     EASCTAGRANFITGEIPLRTGMTTVGQAGADIGIPYQAVTIATVLKSMGYTTGQFGKN
                     HLGDKNKYLPTVHGFDEFFGYLYHLDAMSDPYWFDYPQEWIDKYGPRNLVHCFATDVD
                     DPTEMPRWGKVGKQRIVDEGPLPPFPDMGADHQSWQKGRPAKYDMETFDEVLVDHTKG
                     FMDKAKQDGKPFFIWHNTTRMHVFTYLSAKYKALMNYDSNYNVEEAGMAQLDDSVGVM
                     LKHLDEIGEAENTIVIFTTDNGAEVFTWPDGGMTPFRATKGTVFEGGFRVPAIIRWPG
                     RIKPGSVENGIFSGLDWLPTLAAAAGNSNITDQLLQGAQLGDRTYKNHLDGYNQIDLL
                     TDKGPSARHEIFYFGEAQLGAIRIDDFKFQFIQQPYGWPGEKVTTGMPTIVNLRQDPF
                     ERTPSLRGETLNEEGGGYMNDFLAREFWRFVEVQQKVGALVQTAIEFPPMQAPASFNL
                     QAIKAKIDAVIKSREGQ"
     gene            5147..6781
                     /locus_tag="VGB93_06045"
     CDS             5147..6781
                     /locus_tag="VGB93_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY0145702.1"
                     /translation="MNRRNVLLSGTALAAIYAVASATPQVAQAQETPAPASTGKKPNI
                     LVIFGDDIGQSDISAYTFGLMGYRTPNIDRIAKEGLMFTDYYAEQSCTAGRSSFITGQ
                     SVIRTGLSKVGIPGATVGLQPEDITIAEALKPLGYATAQYGKNHLGDRNEYLPTVHGF
                     DEFFGNLYHLNAEEEPEDPDYPKDPAFRAKFGPRGVLKCKASETDDPTIDPRFGRVGK
                     QTIEDTGPLTRKRMETIDDETTSDAIDFIQRQVKAGKPFFCWMNTTRMHVFTHVRASM
                     RGQSGMPDNEYADGMIEHDGDVGKLLKVLDDLGIANDTIVLYTTDNGPHMNSWPDAAM
                     TPFRSEKNTNWEGAYRVPAMIRWPGHIKPGSIANDIFGSHDWFLTFLAAAGDMDVKDR
                     LLKGWTIGDRTYKVHIDGYDQLAYLTGEEPHSARRGFIYFNDDGQLVAIRFDNWKLVF
                     FEQKTPGTLDIWGEPFTARRLPLYFNLRMDPYERAQITSNEYFAWTAHKVYLLYGAQA
                     IAGAFAQSLQEYPPRQKPQSFNLDQIMDNLKQPTDD"
     gene            6924..7925
                     /locus_tag="VGB93_06050"
     CDS             6924..7925
                     /locus_tag="VGB93_06050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0145703.1"
                     /translation="MAIFLTFALTSWLSGAGSAIADPLPSWNDGPVKQSIVKFVSRVT
                     TQGGVDFVPIAERIAVFDNDGTLWCEQPYYFQLAFALDHIKALAPQHPEWEHQQPFEG
                     VLQGDVKALAAAGEKGLLQILAVTHAGMTTEEFSRIVADWIATARHPRFKRPYIELVY
                     RPMIELLTYLRANDFKTFIVSGGGVDFMRPWTEKVYGISPEQVVGSYGLTKFEMDKNG
                     IPAFRKLPKIEFIDDGPGKPVGINRFIGRRPIFAFGNSDGDQQMLEWTAAGNGVRFMG
                     LVHHTDAIREYAYDRKSRVGKLDKALDEANRRGWTIVDMKHDWRVVFVPVTPDRSQP"
     gene            7992..>8179
                     /locus_tag="VGB93_06055"
     CDS             7992..>8179
                     /locus_tag="VGB93_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope protein"
                     /protein_id="HEY0145704.1"
                     /translation="MMSKRVLTALLALSLSAFALSGAAAQSISPDEARAIAKEAYIYG
                     FPLVDSYRIQYSYFVDHD"
BASE COUNT         1889 a         2201 c         2382 g         1707 t
ORIGIN      
        1 accccagtcg aaatgcatat ccacgccgtt ttgatattgc gtctgcgggt tctcgtaatt
       61 gtaagtgaag ccgagcgtgc ctgacaattc attgcccgtt tgcggattga aataagtata
      121 tgcaccgccg gcatcggtgg cagcatggcc aattccaaga ttaacgaggt tgtcgggatt
      181 atagacaccg atcggaatgt tgctcgtcac gtaagccata taattgtcca cgcccgcatt
      241 ccaacgcagc gagaacatgg gcaagaggtc gccaaaccca aggacattat cgctcgtacc
      301 tcgcgagacg gtaaattccc tgggaccgac gacgccattc aacgtcgcat tgacccccgc
      361 gaagctgccg ccatagggga tcgccatcgc gatcattgcc tgggcgccta aaacgggggt
      421 cgagaacgtg tagctcggta ttgccagata gaggtcggcc cggccgtcga gccttgcatt
      481 tagattgccg ttgaagttgg cggtaatgtc gcctcgcgat acttgccgcg caaagacaac
      541 gcttccgccc gcgccgaccg atggcgcata aaagatattc gccaaggcga aacccggcgt
      601 ttgcggcgtt gccgcaagac ttccaaagaa acccgggacc cagaggctga tgccggtctc
      661 atcggcgcgc gcgatacacg aggaagctat caaaagcaga cctgccaggg caaaagctga
      721 cggcctagaa aatgctttct gccaaacact ccggcaatgt ttcgaaactt tgaacggcgc
      781 acaaaggggg gattccatgt tgccctccac cagctgccaa tcattattat gcgtgggagg
      841 gtaggcctct cgtgaagcga cggccatggg cgaataagaa gtttcgtcac gagatcacgc
      901 ttttcaagcg gccgtgtcga atgagtgaca gcgtgattgc aatagccgcg atggctcgct
      961 tacacgaaaa aaatgcgttc agtagactcg ccgatgcacc acaacgcccc ggcgatagta
     1021 accgtgatgg taaggtgcgt ggacgccaac agccatgacg cccgacgcaa ccggcgcgat
     1081 agatcccgcg cgcacaaacg aatgcgctgg catcattcgt gataaaagtg atgaaaacca
     1141 acccgagcgc caaagtagaa atgagaatct tcattgagat cactccaaaa gtgttggcga
     1201 cagaatgaat gcggctatgg acgatcggat taatcccgga gttagttgcc ggctgccttg
     1261 gaggcagcaa tcgcttgttg caccttctcc ttgagatcac cgaggctaaa gctggcgcca
     1321 gctttagcct cggaggatat tcgatcaagg acggcacgta tttggcgatg atctgcgcgg
     1381 gtagttgcgg cccagatatt gcgcagagaa cgtgatagcg acgtttatgg cgcatgatac
     1441 atgcgtcaca agagggaagc atatctgcaa tacatgtacg cgatgatcgg acgataacga
     1501 accacgttgg aaactgtttt aagaaaatcc gtgaagatta ttcgttttcg cagttgcgaa
     1561 gctttcagca cgacttatga atgcgaagaa atatctggtg cgcggcgatt aatcggaata
     1621 gcctatactc aagttttgaa tgtttgatac ctcgtcatat tgcggaggcg aacatgaagc
     1681 ccaatgcttt atctcttact ttaggcctgt gcgcagctgt gatgctgatt gcgcccgacg
     1741 tcgtacaagc ccgcggcggc tttcatggcg gcggctttca cggcggcgga agaggcttcc
     1801 atggtggcgg gggacgctgg gcgggcggcg ggggccattg gcacggtggt ggcggacgtt
     1861 gggccggcgg tggtggccat tggcgtggcg gcggtggata ttggcgcggc ggtcgctggt
     1921 acggcggcgg tggctggggc tggggcgcag cggctgctgg cgcggcggtg ggagctgcgg
     1981 ctgtcggcgc tgcggcagcc agttcttgct accagcagca aaatgtctgg aatggctatc
     2041 aatatgtccc gcaatgggtg cgggtttgct gagcccttcc ggtgcataga aggcgtgacg
     2101 actttaacaa ggctatcgag gcggcgggtc gtcaatgtga atatctttgc tgacatctcg
     2161 tcggaaattt tcgctcgctg aaccaagttc agcgcggatc tgagtctttg tttgcgggac
     2221 cgggcgtttc ggttcccgcg caaacatcgg agccaagacg cgggaatgaa aatatccgag
     2281 ccagtccatc gagcgattga agacgagacg gccccgtccg atatggtctt tattcccggt
     2341 gcgatgtttc gcatgggatc gaataaacac tatcccgagg aagcgccagt tcaccgcgtt
     2401 acggtcgatg gattttggat cgacacaaca cctgtcacca accggcaatt tgacgacttc
     2461 gtccgcgcga ctggctatgt gacgttcgcg gagatagcgc ccgattcgaa agattatccc
     2521 ggtgcgctgc cgcatatgct ctatgcaggc tcgcttgtct tcacgccccc gtcgcatcgc
     2581 gtcgatttgc gcaactgggg cgaatggtgg cgttttttaa agaacgcgga ttggcgtcat
     2641 ccctacgggc cgggaagcgg cattgatggg cttgacgatc atcccgtcgt tcatattgcg
     2701 catgttgacg cgctcgccta tgccaaatgg gtcggtaagg atttgccgac ggaggccgaa
     2761 tgggagttcg ctgcccgcgg tggtctcgac ggtgcggaat acgcatgggg tgacgaattc
     2821 acgcccggcg gcaaatatat ggcgaatacc tggcacggcg aatttccgcg cgagaatctg
     2881 aaactcgatg ggtatgaccg cacatcgccc gtcaaagcgt tcccaccaaa cggctacggc
     2941 ctccacgata tgatcggcaa tgtctgggag tggaccaatg attggtttgc ccagaagcat
     3001 cggcccgacg cgcaaaaggc ctgctgcatt cccgaaaatc cgcgtggagg tcgagccgac
     3061 gaaagtttcg atcccgcgca accaaaaatc aaaatccccc gcaaggcgat taagggtggc
     3121 tcgcacctgt gcgcgcccaa ttattgccgg cgctaccgcc cggcggcccg acacgccgaa
     3181 gcggtggaca cgtcaaccag tcatcttgga ttccgatgcg tctttcgaaa aggagcagcg
     3241 ttatgaattc cagcgacaat gaagctgaca aaacgcagaa accaagagaa ggcgtaaacc
     3301 ggcgcagcgt gttgctatcc gggacagcgc tcgccgccgc gtcggcgatt gcatcgggtg
     3361 cgccgcaagt cgcggaggcg cagcagccgc ccgcgtcagg cggcaagcaa ccaaacattc
     3421 tcgttatcat gggggacgat gtcggctggt tcaatattgg cgcctatcat caaggcatca
     3481 tgtccggcaa gacgccaaac ctcgacaaac tcgctgcaga tggaatgcgg tttacggact
     3541 actacgcgga ggcaagctgc acggcgggtc gagcgaattt tataactggc gaaattccgc
     3601 tgcgtacagg catgacgacc gtgggacaag cgggcgccga tatcggcatt ccgtatcaag
     3661 ccgtcacgat cgcgaccgtc ctcaaatcga tgggttatac gaccggacag ttcggcaaaa
     3721 accatctcgg cgacaagaac aaatatctgc cgaccgtgca cggctttgat gagtttttcg
     3781 gatatctcta tcaccttgac gcgatgtctg acccctattg gttcgattac ccgcaagagt
     3841 ggattgacaa atacggtccg cgcaatctcg ttcattgctt tgcaaccgat gtggacgatc
     3901 cgacggagat gccgcgctgg ggcaaagtcg gcaagcagcg cattgtcgat gaaggtccgc
     3961 ttccgccgtt cccggatatg ggcgccgatc atcagagttg gcaaaagggc cgtcctgcaa
     4021 aatatgacat ggagactttc gacgaagtcc tcgtcgatca taccaagggc ttcatggaca
     4081 aagccaaaca ggacggaaaa ccgttcttta tctggcacaa cacaacgcgc atgcatgtct
     4141 tcacctacct gtcggcgaag tacaaggcgc tgatgaatta cgacagcaat tacaacgtcg
     4201 aagaagcggg catggcgcag ttagacgaca gcgtcggcgt gatgttgaaa catctggacg
     4261 aaatcggtga ggcggagaat acgatcgtta ttttcacgac cgacaacggt gcagaagtct
     4321 tcacctggcc cgacggcggc atgacaccct tccgcgcgac gaaaggcacc gtgttcgagg
     4381 gtggtttccg cgttccggcg atcattcgct ggccgggccg gatcaaaccc ggctccgtcg
     4441 agaacggcat attttctggt ctggattggc tgccaacgct cgctgcggcg gccggaaatt
     4501 cgaatatcac cgatcagctt ctgcaaggcg cccaactcgg cgaccgcacg tacaagaacc
     4561 acttggacgg ctacaatcag atcgatttgc taaccgataa gggaccgtcg gcacggcacg
     4621 aaatcttcta tttcggcgag gcccaattgg gggcgatccg cattgatgat ttcaagttcc
     4681 agtttattca gcagccgtac ggctggcccg gcgagaaggt cacaacgggc atgccaacca
     4741 tcgtcaatct tcgtcaggat ccattcgaac gaacgccgtc gctccgtggc gaaacgctta
     4801 acgaggaggg tggcggatat atgaacgatt tcctggcgcg cgaattctgg cgcttcgttg
     4861 aggttcaaca gaaagtcggt gcgttggtgc agaccgctat cgaattcccg ccgatgcagg
     4921 cgcccgcctc gttcaacctg caggcgataa aggccaagat cgatgccgtg atcaagagtc
     4981 gcgaaggtca gtaagcgatg atggaccggc gaaggatcga ttatgaccgc cggcgttcgg
     5041 cgcgtcgagg tcgcccaaaa gtcgagaatc catatgaaat cgcattgaat ggagcaatct
     5101 tatgcgttca gatggttctg gaaataaagg cgcaagtaga gacggaatga accggcggaa
     5161 cgtcctgctg tccggaacgg cgctcgcggc tatatacgcc gtggcgtcag ccacgccaca
     5221 agtcgctcag gcgcaagaga cgccggcgcc cgcatcaaca ggcaagaagc cgaacatcct
     5281 ggtcatcttc ggggatgaca tcggccaatc tgacatcagt gcctatacgt tcggattgat
     5341 gggctatcgg acgccgaaca tcgaccgcat cgccaaggaa ggcttgatgt tcacggacta
     5401 ttatgccgag caaagctgca cggctgggcg ctcgtccttc atcaccggcc aatcggtgat
     5461 ccgcaccggc ctatccaagg tcggcattcc cggcgccacc gtcggactgc agccggaaga
     5521 cattacgatc gccgaagcgt tgaagcctct tggttacgcc accgcccaat acggcaagaa
     5581 tcatctcggc gatcgcaatg agtatctgcc gaccgtgcat ggcttcgatg aatttttcgg
     5641 taatctctat cacctgaacg cagaagagga gccggaggat ccggactacc caaaagaccc
     5701 ggcttttcgc gccaaattcg gaccgcgcgg cgtcctgaag tgtaaagcca gcgaaaccga
     5761 tgatccgacc atcgatccgc gcttcgggcg cgtcggcaaa cagacgatcg aggacaccgg
     5821 gccgttgacg cgcaagcgga tggagacgat cgacgatgaa acgacaagtg acgcgatcga
     5881 cttcatccaa cggcaggtca aagccggaaa gccattcttc tgctggatga acacgacgcg
     5941 tatgcacgtc ttcacgcatg tgcgcgcctc gatgcggggg caaagtggca tgccggataa
     6001 tgaatatgcc gacggcatga tcgagcacga cggcgatgtc ggaaaattac tcaaagtcct
     6061 cgacgatctc ggcatcgcca acgatacgat cgtgctctac acgacggaca atgggccgca
     6121 tatgaattcc tggccggacg cggcgatgac gccgttccgc agcgagaaga acaccaactg
     6181 ggaaggtgcg tatcgtgtgc cggcgatgat tcgctggccc ggccatatca agccggggag
     6241 cattgcaaac gacattttcg gcagccacga ttggtttctg acttttttgg cggcggccgg
     6301 agacatggat gtgaaagacc gtcttctaaa aggctggacg atcggcgatc gtacttacaa
     6361 ggtacatatc gacgggtacg atcagcttgc ctacctcact ggtgaagagc cgcattcggc
     6421 tcggagggga ttcatctatt tcaatgacga cggccagctc gttgcgatcc gtttcgataa
     6481 ttggaagctg gtgttcttcg aacagaaaac ccctggaacg ctagatattt ggggggagcc
     6541 gtttacagcg cggcgtttac ctttgtattt caatcttcgc atggatcctt acgaacgcgc
     6601 gcagatcacc tccaatgaat atttcgcttg gaccgcgcac aaggtctatt tgctctacgg
     6661 cgcccaggcg atcgcgggcg catttgcgca atcgcttcag gaatatccgc cgcgacagaa
     6721 gccacagagc ttcaatctcg atcagataat ggacaatttg aaacagccga cggacgactg
     6781 atcatgagcg gttaaggtca gccggcgaac cggctgaccg tgtcgaccct ggcccatatg
     6841 gcaaggacca gcgaaacagc caaaggctta gacgaccgat gcgagtttgc gtcaggaaaa
     6901 attgtctgaa ctttcgttcg cttttggcga tcttcctcac gttcgctctt acgagttggc
     6961 tgtcgggcgc aggcagcgcc attgccgatc cactcccatc gtggaacgat ggccccgtaa
     7021 agcaatcgat cgtaaagttt gtctcgcgcg ttacgaccca aggcggcgtt gatttcgttc
     7081 caattgccga gcgtattgcg gtcttcgaca atgatgggac gttatggtgt gagcagcctt
     7141 attattttca gcttgccttt gcgcttgatc acattaaggc gcttgcgccg caacatcctg
     7201 aatgggagca tcagcaaccc ttcgagggcg tgctgcaagg cgacgtcaaa gcattggctg
     7261 cggcgggcga gaaggggcta ctccaaatcc ttgccgtaac gcatgccggc atgacgacgg
     7321 aggaattttc taggatcgtc gcggactgga ttgcgaccgc gcggcatccg cgattcaagc
     7381 ggccatatat agaactcgtt taccggccga tgatcgagct gctcacatat ttacgcgcta
     7441 atgacttcaa gacattcatt gtctcgggcg gcggcgttga ttttatgcgg ccttggaccg
     7501 agaaggtcta cggcatatcc cccgagcagg ttgtcggttc atacggcctg actaaattcg
     7561 aaatggataa gaacggaata cctgctttcc gaaaacttcc caagattgaa tttatcgatg
     7621 atgggccagg aaagcccgtc ggcatcaacc gtttcatcgg acgccgcccg attttcgcct
     7681 ttggaaattc ggacggtgat cagcagatgt tggaatggac ggcggctggc aatggtgtgc
     7741 gcttcatggg actcgttcat cataccgatg cgatacgcga atacgcctat gaccggaaat
     7801 cgcgtgtcgg gaagctcgat aaagccctcg acgaggcaaa tcgacgtggg tggacaattg
     7861 tcgatatgaa gcacgactgg cgcgtcgttt tcgtcccggt gactcccgat cgaagtcagc
     7921 cctgatcgtt tcggatagtg atcagccccg atttcgtgtg acgagatctc cggcggcacc
     7981 atttggagga aatgatgagc aagcgtgttt taactgctct cctcgctttg agccttagcg
     8041 cgttcgcgct gagcggggcg gccgcgcaat cgatctcacc agatgaggcg cgtgccattg
     8101 cgaaggaggc ctatatttac gggttcccgc ttgttgacag ctaccgcatt caatattcgt
     8161 atttcgtgga ccacgacag
//
LOCUS       DASWOG010000296        12839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_244869,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000296 DASWOG010000000
VERSION     DASWOG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244869"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VGB93_06060"
     CDS             <1..785
                     /locus_tag="VGB93_06060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145705.1"
                     /translation="LSGIMLARPAALFIAGYFGWRTVFFAAAALMFVTGVALARAMPR
                     YQPKTGHRYGQVLASMIGILRDRPPVRWRTAYQGLMFFAFNMFWTTVPIFLAQKFGLS
                     EQAIALFALAGAGGALAAPIAGHLADRGYIGVATGGAAAVLGLSFLGTIGVTGIHGLI
                     WLAVLAVLIDAAVQVNQVVGQNVIFDVPPEIRGRVNAIYMTLVFSCGALGSLIGTLLY
                     AESGWRAAAICGAVVGLLGFSLQIVERFLANKKGVASVRHAD"
     gene            complement(807..3107)
                     /locus_tag="VGB93_06065"
     CDS             complement(807..3107)
                     /locus_tag="VGB93_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0145706.1"
                     /translation="MARQRQVPGVKRYNAPAGGWGALKATAKAVREQMDAGSAGVTLF
                     RTNKPDGFDCPGCAWPDKEHTSTFQFCENGAKAVTWEATKKRVSPEFFAHHTVSELWK
                     WTDYELEDAGRLTHPVIYDRATDKYQAISWDAAFERIGEVLRAQNDPNKVEFYTSGRA
                     SNEAAFLYQIFAREYGTNNFPDCSNMCHEATSVGLPHSIGLGKGTVSLEDFDHCDLIL
                     SFGHNPGTNHPRMMGTLHECAKRGVPIIVFNPLRERALERFADPQSAIEMSTFGYENI
                     ASSYYQVKVGGDAAALKGIMKALLALDRAPGRNATVIDHDFIAEHTNGYEALVADLDA
                     TTWDDIEFASALKRRDLESVAAAYSQSNATILPYGMGITQHTKGTENVQQLANLLLMR
                     GQIGKPGAGICPLRGHSNVQGDRTVGITEKPGADMLARIESTFGFVPPTHHGHDAVAA
                     MQAIAAGRSKVLICLGGNLAVALPDPAQSFPAMCQLDLGVHIATKLNRSHLLVGKESI
                     ILPCLGRTERDVQVGGPQVVTVEDSMSMVHASGGKLPPVSEHLRSEPAIIAGMASATM
                     PQSKVAWMGLVEDYDRIRDKIEAVFPDFKDFNARVKIPGGFRLPLPATERIWKTPSGK
                     AEFLLFTGLNEDPIVHDGSVLRLTTIRSHDQYNTTIYGLDDRYRGVFGRRDVVFIHQD
                     DLAAQNIQHGDAIDVETALPNGTPLRLEGFTAIAYNIARGSVAAYYPEANGLVPLEYH
                     DPQSGTPSYKSVPVRIMRHAGSSAAA"
     gene            3345..3773
                     /locus_tag="VGB93_06070"
     CDS             3345..3773
                     /locus_tag="VGB93_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0145707.1"
                     /translation="MGNSYGAEAAGDAILLAARICLVLLFLIFGWQKLTSFGGTVAYF
                     SHTGVPLPQIAAAVAVTMEFFVGLAIALGFFTRPLALLLALYTLATAIIGHHFWSLSG
                     SAQFENEINFFKNVSIIGGLLLLYVAGAGRFSVDHKIRSA"
     gene            complement(3778..4758)
                     /locus_tag="VGB93_06075"
     CDS             complement(3778..4758)
                     /locus_tag="VGB93_06075"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0145708.1"
                     /translation="MGELIDGVWVTNAMDSVVKDGVLHRPPSIFRNWVTADATAPAGA
                     RGYKAEAGRYHLYVSLACPWAHRTLIMRRLKGLAGMISISIVNWLMAEDGWTFAPGEG
                     VVPDTINGADKLRDIYVLADAHCNSRVTVPVLWDKETKTIVSNESSEIIRMFNSAFDH
                     LGAKAGDYYPEPLRAEIDAVNARIYPGLNNGVYMAGFARKQEAYEEAAERVFATLDWL
                     ERRLSRHDYLVGDTLTEADIRTFTTLVRFDAVYYGHFKCNWRAIADYPAAWNFTRRIY
                     QHPDIRPTVNFLHIKRHYYMSHRWINPTGIVPIGPERDFDAPIERLQSAS"
     gene            complement(4751..5806)
                     /locus_tag="VGB93_06080"
     CDS             complement(4751..5806)
                     /locus_tag="VGB93_06080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0145709.1"
                     /translation="MEIGIDSFAANVPDPVTGRLTAPAIRMADLIDEIEVADRVGLDV
                     FGIGEHHRPEFLDSAPVVILAAAAARTKTIRLGSAVTVLSAADPVRVFQQFATLDLIS
                     QGRAEIIVGRGSFSEAYPLFGFKFEDYDDLFVEKLHLLLRIRDATEVTWSGRFRPPLE
                     GQGIYPRPVQAKLPIYVGVGGTPESFARAGTLGLPLMVAIIGGEFRRFRPLVDLYRDA
                     GRRAGHPPEVLQVGLHAIGFVAETAQEARDTFFPGWLTMFGAIGRERGWSPPSRGQFD
                     AMAGPHGAYLVGDLESVAAKMLRASEALGGVTRITLQMSVASLDHKDMIRSIELLGTE
                     VAPRLRAGIQGGEPSHG"
     gene            complement(5827..6402)
                     /locus_tag="VGB93_06085"
     CDS             complement(5827..6402)
                     /locus_tag="VGB93_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0145710.1"
                     /translation="MTKFKWRAGFAAAMLMAAHAAFAAGAPVWTIDPAKSKLGFSGSQ
                     TGQPFSGNFGRFNAAIAFDPAHLDASQIKVTADLASAATGDPQRDGALPGDDWFDISQ
                     FPQATFVSDKVLKKPDGTYEADGNLTLRGATHPLALPFTLDINGTSARAKGHVSLVRT
                     KFGVGQGQWATGDYVALDVGIDIDIVATRAK"
     gene            complement(6399..7010)
                     /locus_tag="VGB93_06090"
     CDS             complement(6399..7010)
                     /locus_tag="VGB93_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0145711.1"
                     /translation="MVTTARAEKGNRYTAVAIFLHWVMALGILALIVIGLVMTHVKLK
                     PFDLFKLYQLHKSIGITILLAAFLRLAWRHMHKPPALPAAMPPAERKAAEATHLLLYF
                     YLFVLPLTGWAVVSAAVLNIPTYLYGIIPWPHLPILPTLHNKKPVEHVLALIHTYLAW
                     TLIAVLVLHTGAALRHHLILRDDILTRMLPWRRGRAAITGKSR"
     gene            complement(7014..7631)
                     /locus_tag="VGB93_06095"
     CDS             complement(7014..7631)
                     /locus_tag="VGB93_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0145712.1"
                     /translation="MKTHILAARALATALAFAAVSAQAQAPARDPAAVKAGTYKVEPY
                     HTQVSFTLSHFGLSEFSGFFSGASGSLKLNPANPAADKLDVAIPVDSVLTTVPKLDGE
                     LKGDKWFDAAKYPTAEFTSTKVTLAGKASAIISGTLTLHGVTKPITLKAHLVGAGVNP
                     IDKAYTVGFSATGIVKRSAFGVSAYVPYIGDDVHLTIAGVFELQQ"
     gene            7789..8694
                     /locus_tag="VGB93_06100"
     CDS             7789..8694
                     /locus_tag="VGB93_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0145713.1"
                     /translation="MLDGVSLDQLRTCIAAVEEGSFSGAARKLHRAQSVVSEMVSALE
                     AQIGVPLFDRSGRYPKLTGAGTVLLADARGIVSGVDSMKARARGMSEGLEPELSVVVD
                     VFMPIEAITAAAKEFRVKFPDTPLRLYVEALGGVFQPLLDGRASLGIAGSLPFMPSSI
                     MSEALTEFRLVIVASRDHPLASYEGPIPKTEVARHVQLVLTDRSTLSSGREFGVISPS
                     TWRLSDLFAKHAFLLNGLGFGGMPLHTVRQDLESGRLVELDIEGVPATGLILPMHAVY
                     QTTAPPGPAGRWLIERLKTCPSQIV"
     gene            complement(8691..9248)
                     /locus_tag="VGB93_06105"
     CDS             complement(8691..9248)
                     /locus_tag="VGB93_06105"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0145714.1"
                     /translation="MENIDPGKAYLLLESGPIVLVTTHHGDHANIMTMGFHMMMQHAP
                     PLIGCIIGPWDYSHKALKETGECVIAIPTVDLARKVVEIGNCTGAETDKFAEFKLTPK
                     KASKVGAPLIAECLANIECRVADATLVGKYNLFILDAVAISIDRARKERRLLHHNGDG
                     TFTIDGRKLDLRNLMIQWKQFQVPL"
     gene            9289..9900
                     /locus_tag="VGB93_06110"
     CDS             9289..9900
                     /locus_tag="VGB93_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0145715.1"
                     /translation="MPKYLLMLIALVAFVAAAPFAEADQKPDLVTSILGDPAAPVGGN
                     PKGDITIVAFLDYNCPYCRRATPDLDKFIASDGHVKVIYKDWPILAQSSIIAAKVALA
                     ANYQGKYQQAHDALMAITLRPATLPLIKAAVQSAGIDVTQLNKDLAAHNSDIGALLQR
                     NIAEANALHLQGTPVFLVGPYLIAGTLDEAGFRNAVARARAAK"
     gene            complement(9916..11859)
                     /locus_tag="VGB93_06115"
     CDS             complement(9916..11859)
                     /locus_tag="VGB93_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0145716.1"
                     /translation="MTFKDIRWHLWRRAQLWRHAHRDDEFYNYLVGSLFTSQSSLNSA
                     NFMGTAIALSGFALTKDRLFLLLTALALISGLGRTILFRSFIRNKDKLKTRADGAYYE
                     HRFFLFSFLFTAAFGMSCYELIQYPGAQAIANGAAVGYAMGVVARNAGRPSIVITQVM
                     LMIVPMIIGFGSMHNTYGNATALLLVGTIITTLAVTVSLHKNVIAVYNADKATQHLAM
                     YDSLTGLMNRYTFGEQIAHAIATQPNEGFAILYIDLDRLKDINDTLGHTAGDAVIIEV
                     ARRLRAVTRGGDLIARFGGDEFLVKISGADLAETNRIVERIVAALTQPQSVDGKVFAP
                     SASIGVALFPENGQIAGDIIKKADIALYEAKRAGGKTFRVFAPDMEHDLHTQRILRSE
                     IQIAIDRHEFVLHYQPIYELGSRNIIAVEALLRWNHPTRGLLRPDAFIGVAEETRAII
                     DIGEQVLDAACRMAVRLPEHIAVAVNLSTIQFCQPERLIAAVRGSLQRAGLRPNRLHL
                     EITESLLLANTPSARKTLETLTGTGAKLALDDFGTGYSSLSYILDFPFSKIKIDKKFT
                     GSLFLNTASPAIIKAVAQLAGDLSLEVVVEGIETPEQEAFVRTLGPTQGQGYLFSVPL
                     ASEELLRRLARQTGDWQPMSLAS"
     gene            complement(12006..>12839)
                     /locus_tag="VGB93_06120"
     CDS             complement(12006..>12839)
                     /locus_tag="VGB93_06120"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0145717.1"
                     /translation="WVTLAPEPKPVFTLGLVDSLFRLQKAVIALWGKESYARSPIRFF
                     VYRSKGPIFTLGGDLDFYLDCIAKGDRAGLLEHARLSIEDVIGNASSLSGTAITMVTI
                     EGKSIGGGVDAQVSCNIAIAEEHTSFSYPEVKFNHFPVSASAVLSRRVGNRIALKLLS
                     NGTESTASEFEALGALDAVTASGEGEAWIRKYASETLPIHAARLGLFEAFYRPQAEAF
                     RSELGYLAAAWTDHMLRLSPMEISRLQRISATQDRMLQRLYSQTKGSEMRTKSLEPGL
                     V"
BASE COUNT         2491 a         3723 c         3975 g         2650 t
ORIGIN      
        1 tgctgagcgg catcatgctg gcacgcccgg ccgctctctt tattgcaggc tattttggct
       61 ggcgcaccgt gtttttcgcc gcggccgcgc tgatgttcgt aacgggtgtt gcattggcgc
      121 gcgcgatgcc acgctatcag cctaagaccg gtcatcgcta tggtcaggtt ctggcttcga
      181 tgatcggcat tttgcgcgac cgccctccgg tgcgctggcg gacggcctat cagggcctga
      241 tgtttttcgc gttcaatatg ttctggacga cggtgccgat cttcctcgcg cagaagttcg
      301 gcttgagcga gcaggcgatt gcgctttttg cgctggcggg ggccggcggt gcactggctg
      361 cgccgatcgc gggccatctg gccgatcgcg gctatatcgg cgtcgcgaca ggcggcgctg
      421 ctgccgtgct cggtctttct ttcctcggca ccattggggt caccggcata cacggcctca
      481 tttggcttgc cgtgctcgct gtgctgatcg acgctgcggt gcaggttaat caggttgtcg
      541 gccaaaatgt cattttcgac gtgcctccgg agatccgcgg gcgcgtcaac gcgatctata
      601 tgacgctcgt tttcagctgc ggggcgctcg gctcactgat cggcaccttg ctttatgcag
      661 agagcggatg gcgggcggcg gcgatctgtg gcgcggtcgt tggcttgctg ggattttctc
      721 ttcaaatcgt cgagcgtttc ttggccaaca aaaaaggcgt ggcgagcgtc cgccacgccg
      781 attgatttat ctgagacacc gcgccgtcag gccgcggcgc tgcttcccgc gtggcgcatg
      841 atccgcactg gcaccgactt ataggacggc gtcccgcttt gcgggtcgtg atattcgagc
      901 ggtacgagac cgttcgcttc gggataataa gccgcgaccg aaccgcgcgc gatattatac
      961 gcgatggcgg taaagccctc gagccgcagc ggcgtgccgt tgggcagcgc cgtctcaaca
     1021 tcgatcgcgt caccgtgctg gatgttctgc gcggcgaggt cgtcttgatg aatgaagacc
     1081 acatcgcgac ggccaaagac accgcgatag cgatcgtcaa gcccgtagat cgtcgtgttg
     1141 tattgatcat ggctgcggat cgtcgtcagc cgcaggacac tgccgtcatg cacgatcgga
     1201 tcttcgttca ggcccgtgaa caggaggaat tcggccttgc ccgagggcgt cttccatatc
     1261 cgttcggtcg caggcaacgg caggcggaac ccaccaggga tttttacgcg cgcgttgaaa
     1321 tctttgaaat cgggaaacac ggcttcgatc ttgtcgcgga tgcggtcgta atcctccacg
     1381 aggcccatcc aggcgacttt gctctgcggc atcgtggccg acgccatgcc ggcgatgatc
     1441 gcgggttcgg agcgaagatg ctcggatact ggcggcagtt tgccgcctga agcatgcacc
     1501 atcgacatcg aatcctcgac agtgacaacc tgcgggccgc ccacctgaac gtcccgctcg
     1561 gtgcggccaa ggcacggcag aatgattgat tccttgccga cgagcagatg cgaacgattg
     1621 agcttggtcg caatgtgaac accaagatcg agctggcaca tcgcgggaaa gctctgcgcc
     1681 gggtcgggca gggcaacagc gagattgccg ccgaggcaga tcagcacttt cgatcggccg
     1741 gcggcgattg cctgcatcgc cgcgacggca tcatggccgt gatgcgtcgg cggcacgaag
     1801 ccgaaagtcg actcgatccg cgccagcatg tcagcgccgg gcttttcggt gatgccgacg
     1861 gtgcggtcgc cctgcacgtt ggaatggccg cgcaatgggc agatgcccgc gccgggcttg
     1921 ccgatctgtc cacgcatcag cagcagattg gcgagctgct ggacgttttc cgtgcccttc
     1981 gtatgctggg tgatacccat gccatagggg aggatcgtcg cattggactg cgaataggct
     2041 gcggcgactg attcgaggtc gcggcgcttg agtgcgctcg caaattcgat gtcgtcccac
     2101 gtcgttgcat cgagatcggc gacaagcgcc tcatacccat ttgtgtgttc ggcgatgaaa
     2161 tcgtggtcga tgacagtggc gttgcggcct ggggcgcgat cgagcgccag caaagccttc
     2221 atgatgccct tcaacgccgc agcgtcgccg ccgaccttca cctgataata ggacgacgcg
     2281 atattttcgt atccgaacgt cgacatttcg atcgcacttt gcggatcggc gaagcgttcg
     2341 agcgcccgct cgcggagcgg gttgaagacg atgatcggca caccgcgctt ggcgcattca
     2401 tgcagggtgc ccatcatgcg cggatggttg gtgccgggat tgtggccgaa cgagaggatc
     2461 agatcgcaat ggtcaaagtc ttcgagcgag acggtgcctt tgccaagccc gatcgaatgc
     2521 ggcaggccga cgctcgtcgc ctcgtggcac atattcgaac aatcggggaa attgttggtg
     2581 ccgtattcgc gcgcgaaaat ctgatagaga aacgcagctt cattcgaggc gcgtccggag
     2641 gtgtagaatt cgactttgtt cggatcgttc tgggcgcgaa gcacttcgcc gatccgctcg
     2701 aaagccgcat cccatgagat cgcctgatat ttatcggtcg cccggtcgta gataaccggg
     2761 tgcgtcagcc ggccggcgtc ctcaagctcg taatccgtcc acttccaaag ctccgagacg
     2821 gtatggtggg cgaagaattc gggtgagacg cgtttcttgg tcgcctccca ggtcaccgcc
     2881 ttggcgccat tctcgcaaaa ctggaacgtg gacgtgtgtt ccttgtccgg ccaggcgcag
     2941 ccggggcaat cgaaaccgtc gggcttgttg gtccggaaga gcgttacgcc ggcagagccc
     3001 gcatccatct gctcgcggac ggccttggcc gtcgctttca gggcgcccca accgcccgct
     3061 ggggcattat aacgcttaac acctggaact tggcgctgac gagccacggc atagtctcca
     3121 tctatgattg tgcgcggggc tgatttgggg tgccctcttt gggcgcgcgc caaatctcct
     3181 cttatgtgca ccgcaaacct aatggcgatg cgcggcaaaa tccaatggga ctctcaaaac
     3241 gtcatataga gaacttctat taagtcgcct tatttgcctg atcgtcagtg gccggctgcg
     3301 ggccttgtgt taagttcgcg ccagcgacgg aagagggggc gactatgggt aattcttacg
     3361 gtgcagaggc tgccggcgac gcgattctgc ttgcggcgcg catttgtctg gttctgctat
     3421 ttctcatctt cggctggcag aaactgacca gctttggcgg gactgtcgcc tatttctcgc
     3481 acacgggcgt gcccttgccg caaatcgccg ctgccgtcgc ggtcacgatg gaatttttcg
     3541 tcggtcttgc catcgcgctc ggttttttca cgcggcctct ggcgctccta ctggcgcttt
     3601 acacgctcgc cacggcgatc atcgggcatc atttctggag cctgtccggc tcggcgcaat
     3661 tcgagaacga gatcaacttc ttcaaaaacg tcagcattat tggcggcctg ctcttgcttt
     3721 atgttgccgg cgccggccgc ttttccgtgg atcataaaat ccgctcggcg taagcgatta
     3781 gctggcgctc tgcagccgct caatcggcgc gtcgaaatca cgctcgggac caatcggcac
     3841 gataccggtc gggttaatcc agcggtggct catataatag tgccgcttga tgtggaggaa
     3901 attgacggtc ggccgaatat cggggtgctg ataaatacgg cgcgtaaaat tccaggccgc
     3961 cggataatcg gcgatcgcgc gccaattgca tttgaaatgg ccgtaataga ccgcgtcgaa
     4021 acgcacgagc gtcgtgaatg tgcgaatgtc ggcttcggtg agcgtgtcgc cgacgaggta
     4081 gtcatggcgt gacaatcgcc gctcaagcca atcgagcgtc gcgaacacgc gctccgcagc
     4141 ctcctcatac gcctcctgct tccgtgcaaa gccggccata tagacgccgt tgttaaggcc
     4201 gggatagata cgcgcgttca ccgcgtcgat ctcggcgcgc agcggttcgg gataataatc
     4261 acccgctttg gcaccgagat gatcgaaggc cgaattgaac atccggataa tctcggagga
     4321 ttcgttcgag acgatcgtct tcgtctcttt gtcccaaagc accggcaccg tcacgcggct
     4381 gttgcaatgc gcatcggcca ggacatagat gtcccgcagt ttgtcggcgc cattgatcgt
     4441 gtccggcacg acgccttcgc cgggcgcgaa ggtccagccg tcctccgcca tcagccagtt
     4501 gacgatagag atcgagatca tccctgcgag ccctttcagc ctgcgcatga tgagcgtgcg
     4561 atgcgcccag gggcaggcga gcgaaacata gagatgatag cgcccggctt ccgccttgta
     4621 gccgcgcgcg ccggccggcg cggttgcgtc agccgtcacc caattgcgga agatcgaggg
     4681 gggtcgatgc aggacgccat ccttgacgac gctgtccatc gcgttcgtca cccagacgcc
     4741 gtcgatcagt tcacccatgc gacggctccc caccttgtat gccggcgcgg aggcgcggcg
     4801 cgacttcggt gccgagaagt tcaatcgacc gaatcatatc tttatgatcg agcgacgcga
     4861 cgctcatctg caatgtgatg cgcgtgacgc cgcctagtgc ctcgctcgcg cgcagcattt
     4921 tcgcagcgac cgattccagg tcgccgacca gataagcgcc gtgcggaccg gccatcgcat
     4981 cgaattgccc gcgcgacggc ggcgaccaac cgcgctcgcg cccgatggcg ccgaacattg
     5041 tgagccagcc gggaaagaac gtatcgcggg cttcctgcgc tgtctcggcg acaaagccga
     5101 tggcgtgaag gccgacttgc agcacctccg gcgggtggcc ggcgcgacgt cccgcgtcgc
     5161 ggtagagatc gacaagcggg cggaaacgcc ggaattcgcc gccgataatc gcgaccatca
     5221 gcggcaggcc caaggttccc gcgcgggcaa aggattccgg cgtgccgccg acgccgacat
     5281 agatcggcag tttcgcctgc accggacgcg gatagatgcc ttggccctcg agcgggggcc
     5341 ggaaacgccc agaccaggtg acttccgttg cgtcgcgaat cctgagcagc agatgcagct
     5401 tttcgacgaa gagatcgtcg tagtcctcga acttgaagcc gaacagcgga taggcttcgc
     5461 tgaaagagcc gcgaccgacg atgatttcgg cgcggccctg cgaaatgaga tcgagcgttg
     5521 cgaattgctg gaagacgcgc accgggtctg cggcgctgag cactgtcacg gcgctgccaa
     5581 ggcggatcgt tttcgtccgc gccgcggcgg cggcaaggat caccaccggg gccgaatcga
     5641 gaaattcagg ccgatgatgt tcgccgatgc cgaagacatc gaggccgacg cggtcggcga
     5701 cctcgatctc atcgataaga tcggccatcc gaattgcggg cgccgtcagc ctgcccgtca
     5761 cggggtccgg aacattggcg gcaaaactgt cgatgccgat ttccacgaca ttctccttcg
     5821 tggcaattat ttcgcgcgcg tggcgacgat gtcgatgtcg atcccgacgt caagcgcgac
     5881 gtaatcgccg gtcgcccatt gcccctggcc gacaccgaat ttggtgcgga cgaggctcac
     5941 gtggcccttg gcacgggcgc tcgtgccgtt gatgtcgagc gtaaagggca gagccagcgg
     6001 atgcgtggcg ccgcgcagcg ttaaattacc gtcggcctca taagtaccat ccggcttctt
     6061 gagaaccttg tctgaaacga aggtcgcctg cggaaattgc gaaatgtcga accagtcgtc
     6121 gccgggcagc gcgccatcgc gctgcgggtc gccggttgcc gcactcgcaa gatcggccgt
     6181 caccttgatc tgcgaggcat cgagatgcgc ggggtcgaag gcgatggccg cattaaaacg
     6241 tccgaaattg ccgctgaacg gctggccggt ttgcgacccg gagaagccga gtttgctttt
     6301 ggcgggatcg atagtccata cgggtgcacc ggcggcgaaa gctgcatggg cggccatcaa
     6361 catcgcggcg gcgaaccctg cgcgccattt gaattttgtc atcgagattt tccagttatg
     6421 gccgcccggc ctcggcgcca cggcagcatt cgcgtcagaa tatcgtcccg caggatgaga
     6481 tgatgccgga gcgccgcacc cgtgtgcagt acaagcaccg cgatcagcgt ccatgcgaga
     6541 taggtatgga tcaaggcgag cacatgctcg accggctttt tgttgtgcag cgtcggcagg
     6601 atgggcagat gcggccacgg aatgattccg taaagatagg tcgggatatt cagcacagcg
     6661 gcagagacca cggcccagcc cgtcaaaggc agcacgaaaa gataaaagta gagcagcaga
     6721 tgtgtcgcct cggccgcttt gcgctccgcc ggcggcatcg ccgctggcag cgctggcggc
     6781 ttgtgcatgt gccgccaggc gaggcgcaaa aacgccgcga gcaggatggt gatgccgatc
     6841 gatttatgca gttggtagag cttgaaaaga tcgaagggct tcaacttcac atgtgtcatc
     6901 actaagccaa tgacgataag ggcgaggatg cccaacgcca tgacccaatg aagaaagata
     6961 gcaaccgccg tgtagcggtt gcctttctcg gcgcgcgcgg tcgtgaccat actttactgc
     7021 tgaagctcga aaactcctgc gatcgtcagg tgaacgtcgt cgccgatata gggcacatag
     7081 gcgctgacgc cgaacgcgct gcgcttgacg attccggtcg ccgaaaagcc gaccgtatag
     7141 gccttgtcga tcgggttgac gccggcgccg acgagatgag ctttcaacgt gatcggtttg
     7201 gtcacgccgt gcagggtcaa ggtgccgctg atgatcgcgc tggccttgcc cgcgagcgtc
     7261 actttggttg aggtgaattc ggcggtcgga tatttcgctg cgtcgaacca cttgtcgccc
     7321 ttcaactcgc cgtcgagctt cgggacggtc gtcagcaccg agtcgaccgg gatcgccacg
     7381 tcgagcttat cggcggctgg attggcagga ttgagcttca gcgagccgga agcgccggag
     7441 aagaagcccg aaaattcgct gaggccgaag tgggagagcg tgaaggaaac ctgggtatgg
     7501 tagggctcga ctttataagt gccggctttg acggcggccg gatcgcgcgc gggtgcctgg
     7561 gcctgggccg agaccgccgc gaaggcgagc gcggtcgcca aggcgcgcgc ggctaaaata
     7621 tgcgttttca tggggacact cctaaggacg cccggatcgc ggcgcgaggg gcgaatttcc
     7681 gttgccgctg atgcggcgcg aatagcgcgg ctttggtctc acgattgcgg tctatttgaa
     7741 tagccgttat atttcgatcc tatccatctg tgtagccgtt agattcttat gctcgatggc
     7801 gtttcgctcg atcaattgcg cacttgtatc gccgccgtcg aggagggcag cttctctggc
     7861 gccgcgcgaa agctgcaccg cgcgcagtcc gtggtcagcg aaatggtcag cgcgcttgag
     7921 gcgcagatcg gtgtgccgct tttcgaccgc agcggccgct atccgaaatt gaccggtgcg
     7981 ggcacggttc tgctcgccga tgcgcgtggc atcgtttcgg gcgtcgattc catgaaggcg
     8041 cgggcacgcg gcatgtccga gggccttgaa ccggagcttt cggttgttgt cgacgtgttt
     8101 atgccgatcg aggcgatcac cgcagcggcg aaagaattcc gcgtgaagtt tccggacaca
     8161 ccgctgcgtc tttatgtcga agcgctggga ggcgtctttc agccgctgct cgacggccgc
     8221 gccagccttg gcattgctgg ctcgttgccg ttcatgccgt cgtcgatcat gagcgaagct
     8281 ctgacggagt tcaggctggt catcgttgcg tcgcgagacc acccactcgc ctcctatgaa
     8341 ggtccgatcc cgaaaacaga ggttgcgcgg catgtgcagc ttgtcctgac ggatcgttcg
     8401 actctttcgt cggggcgtga gttcggcgtc atatcgccgt ccacgtggcg attgtccgat
     8461 ctctttgcca aacacgcttt cctgctcaac ggcctcggct tcggtggcat gccgctgcat
     8521 acggtccggc aggatctcga gagcggccga ctagtcgagc ttgatatcga aggcgtgcct
     8581 gccaccggcc tcatattgcc gatgcacgcc gtctatcaga cgacggcgcc gccggggccg
     8641 gcggggcgct ggctgatcga gcggctgaaa acctgtccca gccaaatagt ttagagcggc
     8701 acctgaaact gcttccattg aatcatcaaa tttctcaggt cgagcttgcg gccgtcgatt
     8761 gtgaatgtcc cgtcgccgtt gtggtgcagc aggcgccgct ccttgcgcgc gcgatcgatc
     8821 gagatcgcga cggcatcgag aatgaagagg ttgtatttgc caacaagcgt ggcatcggcg
     8881 acgcggcatt cgatattggc gaggcattct gcgatgagcg gcgccccgac ctttgacgct
     8941 tttttcggcg tcaatttgaa ctcggcgaat ttatcggtct cggcgccggt gcaattgccg
     9001 atctcgacga ccttgcgggc gagatcgacg gtcgggatcg cgatcacgca ttcgccagtt
     9061 tctttcaacg ccttatggct gtaatcccaa gggccgatga tgcagccgat cagcggtggc
     9121 gcgtgctgca tcatcatgtg aaagcccatc gtcatgatgt tcgcgtgatc gccatggtgc
     9181 gttgtcacca ggacgattgg tccggattcg agaagaagat aggccttgcc gggatcgata
     9241 ttctccatac gcctgcctca cccaacaccg acatcgacgc gagaattcat gcccaaatat
     9301 cttttgatgc tcattgcgct tgtcgctttt gtcgccgctg cgccgttcgc cgaagcggac
     9361 cagaagcccg acctcgtgac gagcattctc ggcgatccgg cggcgcccgt tggcggcaac
     9421 ccaaagggcg atatcaccat cgtcgccttt ctcgactata attgcccata ttgccgccgc
     9481 gcgacgccgg acctcgataa attcatcgcg agcgacgggc acgtaaaggt gatttacaag
     9541 gattggccga tcctcgcgca atcctcgatc attgcggcga aggtcgcgct cgcggcgaat
     9601 tatcagggca aatatcagca agcgcatgat gcgctcatgg ccatcacatt gcgtccggca
     9661 acgctgcctc tgatcaaggc cgctgttcaa tccgccggca tcgatgtgac gcaactgaat
     9721 aaggaccttg ccgcgcataa cagcgacatc ggcgcgctgc tgcaaaggaa tatcgccgag
     9781 gccaacgcgc tgcatctgca gggtacgcca gtctttcttg tcgggcctta tctgattgcc
     9841 ggcacactcg acgaggcagg cttccgcaac gctgtggccc gagcacgggc ggcaaaataa
     9901 tcgctgtcag agatttcagc ttgccaagct catcggctgc caatcgccgg tctggcgggc
     9961 gagccgtctg agcaattctt cgctcgcaag cggcacgctg aacaaatagc cttggccttg
    10021 cgttgggccg agtgtgcgga cgaaagcttc ttgctctggc gtttcgatac cctcgacaac
    10081 gacttcgagc gagagatcgc cggcaagctg cgcgacggct ttgatgatcg ctggcgatgc
    10141 ggtgttgagg aaaaggctgc ccgtgaattt cttgtcgatt ttgattttgg agaatggaaa
    10201 atccaggatg taggacaagg aagaatagcc ggtcccaaaa tcgtcgagcg ccaatttggc
    10261 gccggttccg gtgagcgtct caagcgtctt gcgcgcggat ggcgtgttcg ccagcagaag
    10321 gctttcggtg atttcgagat gcagacggtt cgggcgcagt cccgcgcgtt gcagactgcc
    10381 acggactgca gcgatgaggc gctcgggctg gcaaaactgg atcgtcgaaa gattgactgc
    10441 gacggcaatg tgctcaggca gccgcacagc cattcggcag gccgcgtcaa gcacctgctc
    10501 gcctatgtcg atgatcgcgc gtgtctcctc tgcgacgcca atgaaggcat ccggccgtag
    10561 caggccgcgg gtgggatgat tccaccggag cagagcttcg acggcgataa tatttctgga
    10621 gccaagctcg taaatcggct gatagtggag aacgaattca tgccggtcga tagcaatttg
    10681 aatctcgcta cgcaggatgc gctgcgtatg caaatcgtgc tccatgtccg gtgcgaaaac
    10741 acggaatgtc ttgccgccgg cacgcttggc ctcataaagc gcgatatcgg ctttcttgat
    10801 aatgtcgccg gcgatctgtc cattctcagg aaaaagcgca acgccaatgc tggccgaagg
    10861 cgcaaagact ttgccgtcta cgctttgcgg ctgcgttaag gctgcgacga tgcgctcaac
    10921 gatgcgattg gtttcggcca ggtccgcgcc gctaatcttg acgagaaatt cgtcaccgcc
    10981 gaagcgagca atgagatcgc cgccgcgcgt aaccgcccgc aatcgccgcg cgacctcgat
    11041 aatgacggcg tcgccggccg tgtggcccaa cgtgtcgttg atatccttca atcggtcgag
    11101 atcgatatag agaatagcga acccttcatt cggctgcgtc gcgattgcat gcgcaatctg
    11161 ctcgccgaat gtgtagcggt tcatgagccc ggtaaggctg tcgtacattg cgagatgttg
    11221 cgtcgccttg tccgcattgt agaccgcgat cacgttctta tgcagggaga cggtcacggc
    11281 tagcgtggtg atgattgtcc ccaccaacag caaggcggtc gcgttgccat aggtgttatg
    11341 catcgacccg aaaccgatga tcatcgggac gatcatcaac atgacctgcg tgatcacgat
    11401 gctcgggcgc ccggcgttgc gggcgacaac gcccatggcg tagccgacgg cagcaccgtt
    11461 ggcgatggct tgtgccccgg ggtattggat gagttcgtaa caactcattc cgaaagcagc
    11521 ggtgaacagg aacgaaaaga gaaaaaagcg gtgctcgtaa taggccccgt ccgcgcgcgt
    11581 tttaagtttg tccttgttgc gaatgaagct gcgaaaaaga atcgtacgtc cgaggccgga
    11641 aatcagcgcc agcgcggtga gcagaagaaa cagccgatcc ttggtcagcg cgaagcccga
    11701 gagggcaatc gcggttccca tgaaattcgc ggagttcaag gaagattgcg acgtgaacag
    11761 cgagccgacg agataattgt aaaactcatc gtcgcggtgc gcatgtcgcc aaagctgcgc
    11821 ccgtcgccac agatgccagc ggatgtcctt aaacgtcatt gggttgcgaa cgtgcctcga
    11881 gaaatttcgt tcgcaccctg cacatagaat gattgaccgg acgttgtccg gtattattag
    11941 attttaggcc aatttgtatg tgagcgcctc aaaatggatc aaataacaac ggcgcaagag
    12001 cgaattcaga cgagcccagg ctcaaggctt tttgtcctca tttcgctacc cttcgtttgg
    12061 ctgtagagac gttggagcat gcggtcttgc gtggcgctga tgcgctgcag gcgagaaatc
    12121 tccatggggg acaacctcag catgtggtcg gtccaggctg ctgccaaata gcccagctcg
    12181 ctgcgaaatg cctccgcctg cggacggtag aaagcctcaa aaaggccgag ccgcgcagca
    12241 tgtatcggca gcgtctcgct cgcatatttg cgtatccacg cctcaccctc gccggaggcc
    12301 gtaacagcgt cgagtgcgcc caaagcttcg aactctgatg cggtcgattc ggtgccattc
    12361 gatagaagtt ttaacgcgat tcgattgccg acgcgtcggg agagaacggc cgaggcagaa
    12421 acggggaaat ggttgaattt tacttcggga tagctgaacg aggtgtgctc ttccgcgatc
    12481 gcgatgttgc aagacacctg cgcgtcgacg ccaccgccaa tgcttttgcc ctcgatcgtc
    12541 accatcgtga tcgcggtgcc cgagaggctt gaagcattac cgatgacgtc ctcgatagag
    12601 agccgtgcgt gctccaacaa accagccctg tcacccttgg cgatacaatc caggtaaaaa
    12661 tcgagatcac ccccaagggt gaaaatcggc cccttggaac gatagacaaa aaagcggatt
    12721 ggggatcgcg cgtagctctc ttttccccac agcgcgatga cggccttttg caaacggaag
    12781 aggctatcga caagcccgag cgtgaaaacc ggcttcggtt ccggcgcgag agtcaccca
//
LOCUS       DASWOG010000297         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_25041,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000297 DASWOG010000000
VERSION     DASWOG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25041"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..765)
                     /locus_tag="VGB93_06125"
     CDS             complement(274..765)
                     /locus_tag="VGB93_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145718.1"
                     /translation="MTLRPEFRQRINFAKLAVLATLLLAVSSASPARADRCDDLAAQL
                     ASQIDGLKVGITAAKVIYLSHPQTREMSLGCTSRKFSNELYAKSTSRKPKPEFIDLAA
                     GAAAIIFTLPKDEMVKGVSRCLSRMGIFRGDDVSIRYRRLDMNCQRTKTDASIAISRS
                     PDQ"
     gene            1034..>1663
                     /locus_tag="VGB93_06130"
     CDS             1034..>1663
                     /locus_tag="VGB93_06130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY0145719.1"
                     /translation="MNAPTAFPDPKPVPPYKHTPLFPLGADTTPYKKITTEGVRVEKV
                     LGKDMLVVSREALRALSEAAFGDINHYLRPGHLKQLRNILDDKEASDNDKFVAFDFLK
                     NANIAAGGVLPMCQDTGTAIIMGKKGFRVITDGDDEAALSEGARDAYLRRNLRYSQVA
                     PLSMYEEKNTANNMPAQCEIYAEGGSSEIDSAYKFMFMAKGGGSANKSFL"
BASE COUNT          335 a          480 c          520 g          328 t
ORIGIN      
        1 gttgcacgtc cggcggcttg gacgcgctgg ggtcgggcag gttgacgggt gtcggcgctg
       61 aaatgctcga aatgttcatg tcggattctc tggaagattt cttgggacga ttttccgggc
      121 taatgcgtcc gactgtgaag cggcggaagc gaaagcttta tgaatccaga ccgtaaatca
      181 cggcatatcc agcccgatcc cgtcacgtgg tgacgggccg ctcaatgcgt cggctaaaat
      241 tgtagcgatt gcgcctgatg cagccgggcg ccattactga tccgggctgc gggaaatggc
      301 gatcgatgcg tcggtcttgg ttcgctggca attcatgtcg aggcggcgat aacgtatcga
      361 gacatcgtca ccgcggaaaa tgcccatccg cgacaggcag cgcgaaacgc ccttgaccat
      421 ttcgtccttt ggcagcgtga agatgatcgc ggcggcgccg gcggcaaggt cgatgaattc
      481 cggcttgggc ttgcggctgg tgctcttggc gtacaattcg ttggagaatt tgcggctggt
      541 acagccgagc gacatctccc tggtttgcgg atgcgacaga tagatgactt tggcggcggt
      601 gataccgacc ttgaggccgt cgatctggct ggcgagctgg gccgcaagat cgtcgcaacg
      661 gtcggcgcgc gccggcgagg cgctggatac ggcaagcaaa agcgtagcta gcacggccag
      721 ttttgcgaaa ttgattcgtt ggcgaaattc cggtctcaat gtcatcgtaa atgccccttt
      781 gcgccattga agcgtctgcg aacgtctggc gcaagagtgg gtgtttattt gcctgtgatt
      841 ggcaactgtc gcgcgcgcta caggatatcg tccttccgtg tcaggtgatc cgaacatccc
      901 ggaaaagcat ttgtgggccg cccggaaggc cggccagaac ctaccctttt tgtcccaaaa
      961 gtgcttagaa cggtggtatc gccgatgcgt ccgaaaagcc ggagcatccg gcccctcaac
     1021 ctgcgagccc gccatgaacg cccccactgc cttccccgat cccaagcccg ttccgcccta
     1081 caagcacacg ccgctgtttc cgctcggcgc cgataccacg ccgtacaaga agatcaccac
     1141 cgagggcgtg cgggtcgaga aggtgctggg caaggacatg ctggtggtgt cgcgcgaggc
     1201 gctgcgggcg ctgtccgagg cggcgttcgg cgatatcaat cactatctgc ggccggggca
     1261 tctgaagcag ctgcgcaata tcctcgacga caaggaagcc agcgacaacg acaagttcgt
     1321 cgccttcgat tttctcaaga acgccaacat cgccgccggc ggcgtattgc cgatgtgcca
     1381 ggacaccggc accgcgatca tcatgggcaa aaaaggcttt cgcgtcatca ccgatggcga
     1441 cgacgaggcc gcgctgagcg agggcgcgcg cgacgcctat ctgcgccgca acctgcgcta
     1501 ttcgcaggtc gcgccgctgt cgatgtacga ggaaaagaac accgccaaca acatgccggc
     1561 gcagtgcgag atctacgccg agggcggctc ttcagaaata gactcggctt acaaattcat
     1621 gttcatggcc aagggcggcg gctcggccaa caagagtttt ctc
//
LOCUS       DASWOG010000298         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_42909,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000298 DASWOG010000000
VERSION     DASWOG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42909"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1186)
                     /locus_tag="VGB93_06135"
     CDS             complement(74..1186)
                     /locus_tag="VGB93_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEY0145720.1"
                     /translation="MTHENAPAGLARTLNLAGKPRVLQWIALIVVTLFFVAILEQVHL
                     PAALLLGPMVAAIFAAAQAAEIKVPGVAFILAQGTVGAMVARVLKPSILTEIRADWLL
                     FLVAVFAVIAASAALGWLLAWRRVLPGSTALWGSFPGAATVMALMAEAYGADIRLVAL
                     MQYLRVVCVATTASIVAAIWAHGGGPVAPVEWFPHLDPKAFAETLLLAFGGALIAPKL
                     RIPAGSLLLPLALGAWLNGAGIITIQLPPWLLAMSYAVVGWSIGLRFTRPMLVYAARV
                     LPRILLSIVALIVICALFGVVLAKVAHVDMLTAYLATSPGGADSVAIIAASTKVDVPF
                     VMALQTARFVIVLLIGPRLARFVADALSDRMESSGR"
     gene            1305..1616
                     /locus_tag="VGB93_06140"
     CDS             1305..1616
                     /locus_tag="VGB93_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0145721.1"
                     /translation="MPNAYDTLFRTLADPSLRAAVPRSEQTVGALTAQSGISQPAVSK
                     HLGVLKQAGLVRDRHEGRQTHYSAQLSALAPLIDWTNQMAGFWESRFDALEDLLKRMD
                     Q"
     gene            1613..1844
                     /locus_tag="VGB93_06145"
                     /pseudo
     CDS             1613..1844
                     /locus_tag="VGB93_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242336.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
BASE COUNT          423 a          554 c          577 g          290 t
ORIGIN      
        1 agcggtgtgc gcggcgccgc ctggctctgg ccgcgctagg ccatgctccg atattatgaa
       61 ttggcgcgat ttcttatcga ccggatgatt ccatccggtc ggaaagcgcg tcggcaacga
      121 accgcgcaag acgcggcccg atcaacagca caataacaaa gcgcgcggtc tggagagcca
      181 tcacaaacgg gacatcgacc ttggtcgagg cggcaatgat cgcgacggaa tcggcaccgc
      241 ccggacttgt cgcgagatag gccgtcagca tgtccacatg cgccactttc gccagaacga
      301 cgccgaacag ggcgcagatg acgatcaacg cgacgatgga caaaaggatg cgcggcagaa
      361 cacgcgccgc gtaaacgagc atcggccgcg tgaaacgcag gccgatgctc cagcccacca
      421 ctgcatagct catggcgagc agccacggcg gtagctggat cgtgatgatg ccagcgccgt
      481 tgagccaggc gccgagcgcc agcggcaaaa gcaatgagcc ggcgggaatg cgcagttttg
      541 gcgcgatcag cgcgccgcca aaggccagca gcagcgtctc ggcgaaagct tttgggtcga
      601 gatgcggaaa ccattcaacg ggcgcgaccg ggccgccgcc atgcgcccat atcgcggcga
      661 cgatcgaggc cgtggttgcg acgcagacga cgcgcagata ttgcatgagc gcaacgaggc
      721 ggatgtcggc gccataggcc tcagccatca gcgccattac ggtcgcggcg cccgggaagg
      781 agccccacaa ggcggtcgag cccggcagca cacgccgcca cgctagcagc cagccgagcg
      841 ccgcactggc ggcgattacg gcgaagacgg cgactaaaaa caacagccag tcggcgcgga
      901 tttccgtcag gatcgacggt ttcagcaccc gcgcgaccat ggcgcccacc gtgccctgcg
      961 cgagaatgaa ggcgacgccc ggcaccttga tctcggccgc ctgcgcggcg gcgaatatcg
     1021 ccgcgaccat cgggccaagc agcagcgccg ctggaagatg cacctgctcc aaaatcgcga
     1081 cgaaaaagag cgtgacgacg atcagcgcaa tccattgaag aacgcggggc ttgcccgcga
     1141 gattgagcgt gcgggcgagt ccggcgggtg cgttttcgtg ggtcatgtgc ggagataaca
     1201 tatatgaaga taagctgcaa agtttagctt catatatatt ggagctgcga acacataact
     1261 cgcaagttat tgatcagctc ataaccctta agttatcgat caaagtgccg aacgcttacg
     1321 acacactttt caggacgctg gccgatccct cccttcgagc ggctgtgccg cgaagcgagc
     1381 agacggtcgg cgccctcacc gctcagtccg ggatctcaca accggccgtc tcaaaacatc
     1441 tgggggttct gaagcaggcc gggctggtgc gcgaccgcca tgaaggacgc cagacgcatt
     1501 acagcgcgca gctcagcgct ttggcgccgc tgatcgattg gacaaaccaa atggccggct
     1561 tctgggagag ccggttcgac gctctcgaag acctgctgaa aaggatggac caatgaacga
     1621 agcatcggcc gaaacacgct ccgtcgtcgt ggagcaggag attccttttt cgccagaaaa
     1681 aatctggcgg gcgctaacgc aaccgcactg atcgcggaat ggttgatgaa aaacgatttc
     1741 aagccagcct tggagcatcg cttcacgctt cgcggcgact ggggttccgt cgattgtaaa
     1801 gtgctggaag tcgagccgaa taaaacgctg tcttgtacgt ggga
//
LOCUS       DASWOG010000299        12004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_135222,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000299 DASWOG010000000
VERSION     DASWOG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135222"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VGB93_06150"
     CDS             complement(<1..781)
                     /locus_tag="VGB93_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145722.1"
                     /translation="MSALRKGWCPSVLRPMASGDGLIVRVSVRNGRVSPTLARALADL
                     SRRFGNGLLDLSARANLQLRGVREASLLSLQEELRALEVVEADEEPSVARNILASPLA
                     GIDPQTVIDIAPCVRALRERLTRDLIVHQLPQKFGFVVDDGGALSLANEKIDISFFAR
                     QGTGGPRFLICLAGRIVGECAIGELADVALRLVRAFLRLRDEPAEPMAALLRGIGIKK
                     IAESAGLTETALTIKNHAPHPIGAYPIGAHYVLGLGVPFGRL"
     gene            complement(778..4098)
                     /gene="cobN"
                     /locus_tag="VGB93_06155"
     CDS             complement(778..4098)
                     /gene="cobN"
                     /locus_tag="VGB93_06155"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY0145723.1"
                     /translation="MHLLRTTTRTIDEAESAVDLGQTPGAIVFLSFSDSDLGLAAAAL
                     EDRQSADVRLASLAALRHPYSVDLYIESVAAQARFVLVRLLGGLDYWRYGAQELAAAA
                     RRHHFDLALIPGDGRADPRLDVLSTLPVATLRAISSSFDCGGSGNLANLFAWIERRSE
                     GETGQLPEPQSTPACGLFAAACRSGASDAPLGVIVFYRAYLLAGDVAPIVAVANALAA
                     RGLRVLAAYVPSLKDAEAGIWLSELLAKEKPDVILNSTAFAARMDQARSPLESADAPI
                     LQMIHASTSRASWDGDMRGLGAADLAMNVVLPELDGRIITRTISFKEETERSAALQFT
                     RVVHQPDAARIAFVADLAKAWARLRRTKRGERRPACILSDYPAKAGRAAYAVGLDTPA
                     SLVEIATRLKAAAYDIEAIHDGDAIVRTLAEGAPRESLLLPRYEEAFAALPEILRAAI
                     VAAWGAPDVDPHVEHGAFQFRAVRLGKMIVAIQPDRGGPAQHKADYHDARLPPSHFYV
                     AFYLWLRHVERIDAMIQLGTHGTLEWLPGKATALSESCAPEALLGPVPLIYPFIVNNP
                     GEAAQAKRRAGAVIVGHLTPPLGPAGSAGDLAEFEALFDEYASAQQMDPKRAALLAET
                     ILTRAGDTGLAAEGGVTLDAPQDEALAKLDAWLCDIKDMRIAEGLHTFGRQPISGAGV
                     LADTQADAAALARLDACAEREMAGLVAALDGRFVEPGPSGAPARGRLDVLPTGRNLVT
                     IDPRMVPTRTAFEIGKRAAAAVIARHLQDHGDWPRRLVIDLWGSATMRTGGEELAQIF
                     AYLGALPVWNDASGRVTGFTILPPAALDRPRIDVTLRLSGLFRDVFPAQIALMEQLIE
                     AIASLDETADDNPLAATSGDMRRIFGPASGTYGVSLGRMLAEDAATSESDLADAYLAD
                     SGTALGRDEHAAADLFRARVASADAFVHVQDLPGQDVLDSSAFFEHEGGFAAAAKQAG
                     SKASLYHLDATRTERPVVRALSEEIARAVRGRAANPKWIAGQMQHGYRGAAEIAETVD
                     SLYGFAVTSSTVTPRQFELVFDATLGNDDVLAFLMRANPEAGRAIAQKFDQAMRRGLW
                     ACRRNSVAMRLANLLEAA"
     gene            complement(4105..5142)
                     /gene="cobW"
                     /locus_tag="VGB93_06160"
                     /pseudo
     CDS             complement(4105..5142)
                     /gene="cobW"
                     /locus_tag="VGB93_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
     regulatory      complement(5244..5449)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5507..6130
                     /gene="cobO"
                     /locus_tag="VGB93_06165"
     CDS             5507..6130
                     /gene="cobO"
                     /locus_tag="VGB93_06165"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0145724.1"
                     /translation="MTIAQDAPDSDARHKEKMRKRKEAQDQEVAAKTIKKGLLIIHTG
                     RGKGKSTAAFGLLLRALGHGWRVGVVQFIKGARSTGEREILATRFADLVSWHTMGEGF
                     TWETQDRARDIAAAEAAWAKAKTLMEDAQIGLVILDELNVALRYDYLPVDDVVAALTQ
                     RRPGLHVVVTGRNAPQALIDAADLVTEMNAVKHHFAAGVKAQAGIEF"
     gene            6130..7593
                     /locus_tag="VGB93_06170"
     CDS             6130..7593
                     /locus_tag="VGB93_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY0145725.1"
                     /translation="MTARRLMFQGTGSDVGKSLIVAGLARAYRKRGLAVAPFKPQNMS
                     NNAAVTADGGEIGRAQALQARAAGLPPSVHMNPVLLKPQSDVGSQVVVQGRVVGTAKA
                     REYQGWKPKLRAAVLESFETLAAVADLVLVEGAGSPAEINLRANDIANMGFARAVDCP
                     VVLIGDIDRGGVIAQIVGTKAVLDADDSALIRGFIVNKFRGDPTLFDAGMRDIEKYSG
                     WPALGLIPYFVDAALLPAEDSVPRQRFMPSDESRPLKIVTPVLPHIANFDDLDPLAAE
                     PNVNFMMIRHGTPLPGDADLIILPGSKATRADMDALVAEGWDIDIKAHVRRGGKVLGL
                     CGGFQLLGRLIADPDGIEGPPGETPGLGLLDVDTVLGGEKMLLAVAGHDTTDGAPFNG
                     YEMHIGRTYGPDCTRPVHRLDDGRHEGARSASGLVAGTYVHGLFADDHQRRAWIASLG
                     GRGSDLAYEDQIERTLDALAAHLETHLDLDLLLSLAK"
     gene            complement(7586..8557)
                     /gene="cbiB"
                     /locus_tag="VGB93_06175"
     CDS             complement(7586..8557)
                     /gene="cbiB"
                     /locus_tag="VGB93_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEY0145726.1"
                     /translation="MAFASTFGLTAAALALEAIFGYPAFLLRAIGHPIVWIGALIGAT
                     ESQLNRQSSNAQIQKLSGVVALAFWLICVGICGGVVTRLLGRSLFGELVLALLATSLL
                     AQRSLHDHVRAVAAALEHEGIEPARAAVGRIVGRNAAHLDEAGIARAAIESLAENFSD
                     GVVAPAFWLAVGGLVGTMLYKTINTADSMVGHRTPRFVNFGWASARLDDLVNLPAARL
                     AALLFAAAAAFGGLAPAREVWHATRRDAPKHLSPNAGWPEAAIARALSLKLGGPRVYG
                     ENVVDGAFMGDGRRDANAKDIHRALALFRRAAAIQIFAYAALALVFI"
     gene            8770..9825
                     /locus_tag="VGB93_06180"
     CDS             8770..9825
                     /locus_tag="VGB93_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEY0145727.1"
                     /translation="MSVPSRSSALPLGSFALIAVIIAASAGAFAYTAGWLSPNRLTAH
                     KIIAALAPPGGPALGHRRNHAKGICFTGVFDSNGNGAVLSSSQIFTRGQYPALGRFNL
                     ATPNANAADAMVRVRGLGLQISAPNGEVWRSAMIDPPFFPVATVEGFYALQLASKSKD
                     PSAMGTFAAAHPEIKAFGGWAKSAPWTGSYAEEPYKSLDSFIFTDESGAKRAVRWSYI
                     PQAQAVPITQAELSKRGPNYLEQEIAERVGRAPQRWTLMLTVADPSDQTVDPSKAWPK
                     GRRTIKAGELVVQRIEAEADGPCRDVNFDPTILPNGVTVSDDPFPAARSSAYAVSYDA
                     RTAEAKYYPYTQSSSHP"
     gene            9822..10385
                     /locus_tag="VGB93_06185"
     CDS             9822..10385
                     /locus_tag="VGB93_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0145728.1"
                     /translation="MSTDRQRFTVLQRVLHWLMALCLLAMLFIGVGMVSTVMPKYVPL
                     LATHKTLGIAILTLALIRLGVRARYGAPTLPADLPEPMKLAAHLSHYGLYALMIAMPL
                     LGWGMMSAGSYPVVLFGNIHLPPILPPSDDVHTLLWDAHYYLAFAFFALILMHIAAAL
                     FHTLIRRDGVFETMASASFSDDATPAE"
     gene            complement(10509..10889)
                     /locus_tag="VGB93_06190"
     CDS             complement(10509..10889)
                     /locus_tag="VGB93_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145729.1"
                     /translation="MMTALQRVTFEREFDLPVRRRRAGQKALAFTRENRTPAASDSVH
                     PIVVAIPVLTAVWFLAVAWVAFAGGEGSLMLAVVTFITAIFFSLLVGGAAMAGNAAPE
                     SRQTRSLSAFLHGETEVATGRVSG"
     gene            11173..11412
                     /locus_tag="VGB93_06195"
     CDS             11173..11412
                     /locus_tag="VGB93_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145730.1"
                     /translation="MIKSFSVSATCAAILVASLSASSAFASTHHASHRESVLGMNRYY
                     ALVGQSSSSASADAAKFRMPAPTHDDPFSFPVSDQ"
BASE COUNT         2431 a         3734 c         3578 g         2261 t
ORIGIN      
        1 cgagccgtcc aaaagggacg ccgagaccaa gaacataatg cgcgccgata ggataagcgc
       61 cgatcgggtg cggggcgtga tttttaatcg tcagtgcagt ctcggtcagg ccggcacttt
      121 cggcaatttt cttgatgccg atgccccgta gaagtgccgc catgggttcc gcaggctcgt
      181 cacgcagtcg cagaaatgca cgcacgaggc gcagagcgac atccgctagt tcgccaatcg
      241 cgcattcacc cacgatccgg ccggcgaggc aaataagaaa gcgaggcccc cccgtccctt
      301 gccgcgcgaa aaaactgata tcgatctttt cgttggcgag cgaaagtgcg ccgccatcat
      361 ccaccacaaa accgaatttt tgcggaagct gatgcacgat caggtcgcgc gtcagccgtt
      421 cgcgcagcgc gcggacgcaa ggcgctatgt cgatgacggt ttgcggatcg atacctgcca
      481 gcggactcgc gagaatattg cgcgcgacgg aaggctcttc atcggcttcg acgacctcaa
      541 gcgcccgaag ctcttcctgc aaagacaaga gcgatgcttc tctgacgccg cgcaattgta
      601 aattcgcccg ggccgagaga tcgagaagcc cgttgccgaa acggcgcgaa agatcggcca
      661 gtgcgcgcgc aagcgtcggc gaaacgcgcc cgttcctcac gctgacgcgg acgatcaatc
      721 cgtcgccgct cgccatcggc cgcagcacgc tcgggcacca gcctttgcga agcgcgctca
      781 tgcggcctcc aaaaggtttg caaggcgcat cgcgaccgaa ttgcgccggc aggcccaaag
      841 cccgcgccgc atcgcttgat cgaatttctg cgcgatcgcc cttcccgctt ccggattggc
      901 gcgcataagg aaggccagaa cgtcgtcatt gccgagcgtt gcgtcgaaga cgagctcaaa
      961 ctggcgcggt gttactgtgc tgctcgtcac cgcgaagccg taaagactat cgaccgtctc
     1021 tgcgatttca gccgcgccgc gataaccatg ctgcatctgg cccgcgatcc atttcggatt
     1081 ggccgcgcgt ccgcgcaccg cgcgggcaat ttcctcgctg agggcacgaa ccactggcct
     1141 ctcggtgcgt gtcgcgtcga gatgatagag gctagctttg ctaccggctt gttttgccgc
     1201 cgccgcgaag ccgccctcgt gctcaaagaa agcactcgaa tcgagcacat cctgaccggg
     1261 taaatcctgc acatgcacga aagcgtcggc gctcgccacg cgggcccgga acaaatccgc
     1321 cgctgcatgt tcgtcgcgtc cgagcgctgt accgctgtcg gcgagataag cgtcggcgag
     1381 atcgctttcg gatgtcgcgg catcttcagc caacatccgg ccaagcgaaa cgccataagt
     1441 accggaggcc ggaccgaaaa tgcgccgcat gtcgccgctc gtcgcagcga gcggattgtc
     1501 gtccgcagtc tcgtcaagcg atgcgatcgc ctcgatcaat tgctccatca gtgcgatctg
     1561 cgccggaaac acgtcgcgaa aaagccccga gagccgcaat gttacatcaa tgcgcggacg
     1621 atcgagcgcc gcaggcggca gaatggtgaa gccggtaacg cggccggacg cgtcgttcca
     1681 gaccggaagc gcgccgagat aggcgaagat ttgcgccagt tcctcgccgc ctgtgcgcat
     1741 cgtcgccgag ccccaaagat cgatgacgag ccgtcgtggc caatcaccgt ggtcctgcaa
     1801 atgccgggcg atcaccgcgg ctgccgcgcg cttgccaatc tcgaacgccg tccgcgtcgg
     1861 gaccatgcgc ggatcgatcg tgacaagatt gcgcccagtc ggcaaaacgt ccagtcgccc
     1921 acgcgcaggc gcgcctgacg ggccgggctc gacgaaacga ccatcgagtg cggcaacaag
     1981 acccgccatt tcgcgctctg cacaagcatc gagccgcgct aaggccgccg catcggcctg
     2041 cgtatccgcc agcacgcccg cgcccgaaat tggttgccgt ccaaatgtgt gcaggccttc
     2101 ggcgatgcgc atatccttga tgtcgcaaag ccaggcgtcg agcttcgcca gcgcctcatc
     2161 ctgcggcgca tcaagcgtga cgccgccttc cgccgcgaga cccgtgtcgc cggcgcgcgt
     2221 caaaatcgtc tcggcgagca aagcggcgcg cttcgggtcc atttgctggg ccgaggcata
     2281 ttcgtcgaag agcgcctcga attccgcaag atcgccggca ctgcccgcgg gcccaagcgg
     2341 cggcgtcaaa tggccgacaa tcactgcgcc ggcacggcgc ttcgcctgcg ccgcctcacc
     2401 gggattattg acgatgaacg gatagatcag cggcaccggt ccgagaagtg cttccggcgc
     2461 acaagattct gagagcgccg tcgctttgcc cggcagccat tcgagcgtgc catgcgtacc
     2521 gagctggatc atcgcatcga tcctctcgac gtgacgcagc cacagataaa acgcgacata
     2581 gaaatgcgat ggcggcagcc gcgcatcgtg ataatccgct ttgtgttgcg cagggccgcc
     2641 acgatccggc tggatcgcca cgatcatctt gcccagccgc acggcgcgaa actggaaagc
     2701 gccatgctcg acatgcggat caacgtccgg cgcgccccag gcggcgacga tcgctgcacg
     2761 caagatttcg ggcagcgcgg caaaagcttc ttcatatctc ggcagcaata aggattcgcg
     2821 cggcgcgcct tccgccaaag ttctgacgat ggcgtcgcca tcgtgaattg cctcgatatc
     2881 ataggccgcc gccttgagcc gcgttgcgat ctcgacgaga ctcgctggcg tatcgagccc
     2941 caccgcgtaa gcggcgcgcc cagccttggc cggataatcg gacaatatgc aggcaggccg
     3001 ccgctcgccg cgctttgtgc ggcgcaaacg cgcccatgct ttggcgagat cagcgacgaa
     3061 tgcgatccgc gcagcatccg gctgatgaac gacgcgcgtg aattgcaacg ccgcgctgcg
     3121 ttcggtctct tccttgaatg aaatcgtccg cgtgatgatg cgcccgtcaa gctccggaag
     3181 gacgacgttc atcgcgagat cggcagcgcc gaggccgcgc atgtccccgt cccacgacgc
     3241 gcgcgaggta ctggcatgaa tcatttgcag gatcggcgcg tccgcagatt caagcgggct
     3301 tcgcgcttgg tccatgcgcg ccgcaaatgc cgtcgagttc agaatgacgt cgggtttctc
     3361 tttcgccaga agctccgaga gccagatccc cgcctcggca tctttgagac tgggaacata
     3421 agctgccaag acgcgcagac cacgcgcggc caaggcgttg gcaacagcga cgatcggcgc
     3481 gacatccccg gcgagaagat aagcgcgata aaagacgatg acgccgagcg gcgcatcact
     3541 cgcgccagat cggcaagccg cggcgaaaag cccgcaggct ggcgtcgatt gcggctcggg
     3601 caattggcct gtctcgccct cgctgcgccg ttcaatccag gcaaaaagat tggcaagatt
     3661 gccggaaccg ccgcaatcga aactgcttga tattgcgcgc agcgttgcga ccggcaatgt
     3721 cgatagcacg tcgagccgcg gatcggcgcg gccatcgcct ggaatgagcg cgagatcgaa
     3781 gtgatgccgc cgcgcggccg ccgcaagttc ctgcgcgcca tagcgccaat aatcgagacc
     3841 accgagaaga cgcaccagca cgaagcgcgc ctgcgccgcg acagattcaa tataaagatc
     3901 gaccgaataa ggatggcgta gcgccgccaa tgaggcgaga cgcacatcgg cgctttggcg
     3961 atcttccagt gctgccgctg cgagcccaag atcgctatcg gaaaaagaca agaagacgat
     4021 cgcacccggc gtctggccaa gatcgacagc gctctccgcc tcgtcgattg tccgcgtcgt
     4081 cgtgcgcaga agatgcatgg gcgctcagcc gaccgcggcg gcgatcgccg cgcgatcaag
     4141 ccccgtttcg ccgatgacga ccagccggcc ttgacgcgtt tcgtccttat gccacgggcg
     4201 atcgaaattc tgccgaaagc gccgtccgac gccctgcacc agcaggcgca tcggcttgcc
     4261 cgcgacctcg aaaaacccct tcatccgcag cacagcattc tgctcggcaa tggcgctcaa
     4321 cttggcaatg aattgctccg gatcgctgag cggaccaaca ttgagcacga agctttcgaa
     4381 ttcgtcgtga tcatgctcgg gctcgtcgtc atgatgcgac ttgcgtgact cgatctcatc
     4441 ctctgatgca gcttcgagcc cgagcagcaa ttagggatcg accttgcctt cgctcgtgat
     4501 gacgatcttg actgcgcgcg gcagaaccgc tgcgatctgc tcgcgcgccc gcgtcttctc
     4561 atcgtccgtt aatagatcgc ctttgttgag cacgaccatg tcggcgcaca gaagctgatc
     4621 ctcgaagact tcctcaagcg gattatcgtg atcgagcgaa ggatctgcag cgcgctgtgc
     4681 cgcgaccatt tcgggatcat cagcaaatgt gcccgcaagc acggcggcgc cgtcgacgac
     4741 ggcaatcacg ccatcgaccg tgaggcgcga gcggatggtc ggccaatcga aggctttcac
     4801 caatggcttt ggcagcgcca gcccggatgt ctcgatgatg atatggtccg gcttgtcgcc
     4861 gcggctaagc aatgcctcga tcgccgggac aaaatcatcg gcgacggtgc agcagatgca
     4921 gccattggca agctcgatga tgttttcttc cggacatgac gcgacgccgc aggtgcgcag
     4981 tatgtcgcca tcgacgccga catcgccgaa ttcattgatg atcaaggcca ggcggcggcc
     5041 ttgcgcgcgt tcgagcaaat ggcggatcag cgtcgtcttg ccggagccaa gaaagccagt
     5101 gacgatcgtg gtcggaattt tatgcgtatc gagcgccgcc attccatgct ccgcttcatt
     5161 ttgcggggtg aaggaccggc gcacttgagc gcaacgcagc gtgcaaaggc tgcagcattc
     5221 gcctcggggc accccgcccg acgcaaacgg tcaacgaaga cggtaggtct cctggctcgc
     5281 ggctcaacac atatgtcgcc cgccttccca gatcgcctcg cggcaaccca gtggcttcgg
     5341 gcgactgctc accgctcaca gttgcggggg cagctgcggc tttgagcaga cgctcagacc
     5401 gcattccctg ttttccttcc gaagaaggac cgtcatcgcg caccttgcca tcgcccggca
     5461 gccttgtcaa aacagcgcct tccgctgcgg acgctggaga tttcccatga caattgcgca
     5521 agacgcgcct gattccgacg cgcggcataa agaaaagatg cgtaagcgca aggaagcgca
     5581 ggaccaggag gtcgccgcaa agacgatcaa aaaagggctg ctgatcatcc ataccggacg
     5641 cggcaagggc aaatcgacgg cagccttcgg cctcttgctg cgcgcgctcg gacatggatg
     5701 gcgcgtcggt gtcgtgcaat tcatcaaggg cgcgcggtcg acgggcgaac gcgagatatt
     5761 ggcgacgcgc ttcgccgatc tcgtctcatg gcacacgatg ggcgagggct ttacttggga
     5821 aacgcaggat cgcgcccgcg atatcgccgc cgccgaagcc gcctgggcga aagcgaaaac
     5881 gctgatggaa gatgcgcaga tcgggcttgt catcctcgac gaactgaacg tcgccttgcg
     5941 ctacgattat ctgcctgtcg atgatgtcgt cgcggcccta acccagcggc gtcctggcct
     6001 gcatgtcgtg gtcacgggac gtaatgcgcc gcaggccctg atcgatgctg ccgatctcgt
     6061 gaccgaaatg aatgcggtca agcatcattt cgcggcgggc gtgaaagcgc aggccggaat
     6121 cgagttttaa tgacagcgcg acgtctgatg tttcagggca cgggttccga cgtcggcaaa
     6181 tccctgatcg tcgccgggct cgcgcgggcg tatcgcaagc gcggactcgc ggtcgcgccg
     6241 ttcaaaccgc agaatatgtc aaacaatgct gccgtgacgg cagatggcgg cgagattggc
     6301 cgcgcacagg ccttgcaggc gcgggcggca ggtcttccgc caagcgtgca tatgaatccc
     6361 gttctgttga agccgcaaag cgatgtcggt tcgcaggtcg tggttcaagg acgtgtggtg
     6421 ggaaccgcca aggcgcggga atatcagggt tggaagccga agctgcgtgc cgctgtgttg
     6481 gaaagctttg agactctcgc ggcagtggcg gatctggttc tcgtcgaggg cgccggcagc
     6541 ccggccgaaa tcaacctgcg cgccaacgat atcgccaaca tgggcttcgc gcgggctgtg
     6601 gactgtcctg tcgtgctcat cggcgacatc gaccgcggcg gcgtgatcgc acagatcgtt
     6661 ggcacgaaag cggtgctcga tgccgacgat tctgcgctga tccgcggctt catcgtcaac
     6721 aaatttcgtg gcgatccgac cctgttcgac gcggggatgc gcgatattga aaaatactcg
     6781 ggctggccgg cgctgggact cattccctat ttcgtcgatg cggccctgct acccgccgaa
     6841 gacagcgttc cgcgccagcg atttatgccg agcgacgaaa gccggccgct caagatcgtg
     6901 acgccggttt tgccgcacat cgcaaatttc gacgacctcg atccgctcgc tgcggagccg
     6961 aacgtcaatt tcatgatgat ccggcacggc acgcccctcc ccggcgatgc ggacctgatc
     7021 atcctgccgg gatcgaaagc gacgcgcgcc gacatggatg cgctcgtcgc agaaggctgg
     7081 gacatcgata tcaaggcgca tgtccgccgt ggcggcaaag ttctgggcct ttgcggcggc
     7141 tttcaattgc tcgggcggct aatcgccgac ccggatggca tcgaagggcc gcccggcgaa
     7201 acgccgggcc tcggcctgct cgatgtcgac accgtgcttg gcggcgaaaa gatgcttttg
     7261 gccgtcgccg gtcatgacac gacggacggc gcgccgttca atggctatga aatgcacatc
     7321 ggtcgcacct acggacccga ttgtacgcgc cccgtgcaca ggctcgacga cggacgtcac
     7381 gaaggcgcac gttcggcgag cggccttgtc gccggcactt atgtccacgg cctgtttgct
     7441 gacgatcatc aacgccgcgc gtggatcgct tcgttgggcg gcaggggctc tgatctcgcc
     7501 tatgaagacc agatcgagcg aacgctcgac gccctcgccg cgcatctcga aacgcatctt
     7561 gatctcgatc ttttactcag cctcgctaaa taaagacgag tgcgagcgcc gcataagcaa
     7621 aaatctgaat cgcggcggcg cgccgaaaca atgcaagtgc gcggtgaata tctttcgcgt
     7681 tggcgtcacg gcggccatcg cccatgaagg cgccatcaac gacattttcg ccatagactc
     7741 gtgggccgcc gagcttgagg ctgagcgccc tggctatcgc cgcctccggc catcccgcgt
     7801 tgggcgacag atgctttggc gcatcgcgcc gcgttgcatg ccaaacctcg cgcgccgggg
     7861 caaggccgcc aaacgcagcg gctgcggcga aaagcaaagc ggcgagtcgc gcagccggaa
     7921 gattgacgag atcgtcgagc cgcgccgagg cccagccaaa attcacgaag cgcggtgtgc
     7981 gatgcccgac catgctgtca gccgtgttga tagtcttgta gagcatcgtg ccaacgagac
     8041 cgccgacagc gagccagaac gccggcgcga cgacgccatc tgaaaaattc tcggcaagac
     8101 tttcaatggc ggctcgggca ataccagctt catcgaggtg cgccgcattg cggccaacga
     8161 tccggccgac cgccgcgcgt gccggttcaa tcccctcgtg ctccaaggcc gcagcgacgg
     8221 cgcgtacatg atcgtggaga gagcgttgcg ccagcaagct tgtcgccaag agcgcgagca
     8281 ccagctcacc gaagagcgac ctgccgagca gccgcgtgac aacgccgcca caaattccaa
     8341 cgcaaatcag ccagaaggcc aaggcgacaa cgccggagag tttttggatt tgcgcgtttg
     8401 aagactgccg attgagctgc gattccgtgg ccccaatcaa cgcgccaatc cacacgattg
     8461 gatggccgat cgcgcgtaat aaaaatgccg gatagccaaa gatcgcttcg agcgcgagag
     8521 ccgccgccgt gagaccaaac gtactcgcga acgccatctc ttgtgtccgg ttggcgaaat
     8581 cgacatgacg atgtccgcta aatgccggtt ggatgccgcg ccgtcaagct tgccgagact
     8641 agtgatgaat gagtgtcaat gtgggaattg accgcgttcg aagggccatg acatggattt
     8701 tagaatgttg cggttgaatt cgcctagcgg gcgttgcatg ttccgcaagt atctttctgg
     8761 agttgagcat tgtccgttcc gagccgatcc tctgcattgc ccctcggctc attcgcgctc
     8821 atcgccgtga ttatcgcggc gagtgctggc gcgtttgctt atacggcggg ttggctgtcc
     8881 cccaaccgtc tcaccgctca caaaatcatc gccgctttag ccccgcctgg cggccccgcg
     8941 ctcgggcatc ggcgcaacca cgcaaaggga atctgtttta ctggcgtgtt cgactcaaat
     9001 ggcaacggcg cggtactctc ttcatcgcag atattcaccc gcgggcaata tccagcgctc
     9061 gggcgcttca acctcgcgac tccgaacgcc aacgcggccg atgcgatggt tcgcgtgaga
     9121 ggccttggac ttcagatttc agcgccgaac ggcgaagtct ggcgctcggc catgattgac
     9181 ccacccttct ttcccgtcgc aacggtggaa ggcttttatg ctctgcaatt agcctcgaaa
     9241 agcaaagatc ccagcgcgat ggggacattt gcggccgcgc atccggagat caaagctttt
     9301 ggcggctggg caaagagcgc tccatggacg ggaagctacg ccgaagagcc ctataagagc
     9361 ttagactcct tcatctttac agacgagtcg ggtgcaaaac gcgcggtgcg ttggtcatat
     9421 attccacagg cgcaagccgt accgatcacg caagcagagc ttagcaaacg cggccccaat
     9481 tatctcgaac aggaaatcgc agagcgcgtc ggcagagcac cgcagcgttg gacattgatg
     9541 ttgaccgttg cggatccgag cgatcagaca gttgatccga gcaaggcctg gccgaagggg
     9601 cgccgaacga tcaaagccgg agagcttgtt gtgcagagga tcgaggccga ggcagacggc
     9661 ccttgccggg atgttaactt cgatccgacg atcctgccaa acggcgtcac cgtctccgat
     9721 gatccgtttc cggccgctcg ctcctccgca tatgcggtct cctacgacgc ccgtacggcg
     9781 gaggccaagt attatccgta tacgcaatcg agttcgcacc catgagcaca gaccgtcagc
     9841 gtttcaccgt tcttcagcgc gtgctgcact ggctgatggc actttgcctt ctcgcaatgc
     9901 tcttcatcgg cgttggcatg gtctcgacag ttatgccgaa atatgtacct ttgcttgcaa
     9961 cacataaaac gctcggcatt gcgatcctta ctctcgcact gatcaggctc ggcgtgcgcg
    10021 ctcgctatgg cgcgcctacg ctgccggcgg atttgcccga accgatgaag ctggcggcgc
    10081 atctatcgca ttacgggctt tacgcattga tgatcgccat gcccctgctc ggttggggga
    10141 tgatgtcggc cggctcttac ccggtcgtgc tcttcggcaa tatccactta ccgccgatcc
    10201 tcccgcccag cgacgacgta catacgctgc tctgggacgc gcactattat ctggcgtttg
    10261 ccttcttcgc cctcatcctc atgcatatcg ctgcggcgct gtttcacacg ctcattcgac
    10321 gcgacggtgt tttcgagacg atggcgtccg cgtccttcag cgatgacgcc acaccggccg
    10381 aatgatcgcg acaacgtagg cgccgctccg ggcaacggct gcagatcaaa ccgcaacgcc
    10441 ataaaggcga tggccgttgc gccaacggcg attgcaaccg gcatcacagc tgtctgcacg
    10501 agaacctctc agccgctgac gcggcctgtc gcgacctcag tttcaccgtg aagaaacgcg
    10561 ctcaggctgc gggtttgccg ggattcaggc gccgcattgc ccgccattgc cgccccgccg
    10621 acgagcagcg aaaaaaaaat cgcggtaatg aacgtgacga cggccaacat cagcgaacct
    10681 tcgccaccgg caaacgctac ccacgctaca gcgagaaacc agacggcggt cagaaccggg
    10741 atggcaacga cgatcggatg cacgctgtca gaggcagccg gcgtgcggtt ctctcgcgta
    10801 aaagccaaag ccttctgacc agcccgccgc cggcggacgg gtagatcgaa ttcgcgctcg
    10861 aaagtgactc tttgtaacgc ggtcatcata aacttctcct ttatttacca ggcccgttgc
    10921 ctgtcgaaag agaagctagt caggcgcgcc ttatcgttcc aacgaatgat tttgaattgt
    10981 ttgatcggat aatgtgatcg aactcggctt accgcggcag cgagaaccca atgagtttgg
    11041 acgccttgca ttcgatcaac aaaaaatccg gaacaatatg atctgaactt cgcgttggtc
    11101 gcgcgcactt ctgcggcaca taagcggcgg caacggcgct cacatgcgtc ttccaaagcg
    11161 aaggagatta tcatgatcaa gtcattttcc gtttctgcca cgtgtgcggc catccttgtg
    11221 gcgagcctct ccgcctcctc ggcgttcgcg agcacgcacc atgcaagtca ccgcgaaagc
    11281 gtgctgggca tgaaccgtta ttatgcgctt gtcgggcaga gctcgtcttc ggcgtcggcg
    11341 gatgccgcaa agttccgcat gccggcgccg acgcatgacg acccttttag cttcccggtt
    11401 tcggatcagt aacgcgatct tatagtgcag acgccggaag tcttttcttt gctatctgta
    11461 tagagagaag aatttgagca gaaaaagtgg tgaaagaaac aatgccaagc agtccctgct
    11521 tcaccaatgg tagaatttgg aaagtcattc tatttacagt tttaccaaat tacagttcgg
    11581 gttcggacgc ggcttggcct tagctagcca aagatcgctt tatattagct ggttgggaga
    11641 cggctctgtt ggagccgttt cttgggaggc ttgttcaagt gtgatttttg tcacctcggc
    11701 ctaagcaact ttacgtatat ttgcattgca tccggtactc attagccggg aatgcgttat
    11761 tgacctaggc ggctctccat tggggtggag ggccgtttcg tttaccggcc ttcgttgcgc
    11821 gaaaaagaac gaggtcgaga cggttcgttc acgaggctct agcgctcttt gcgttttcgc
    11881 gtgatcgttg cgatcaacgc ctgcgcgggg tgccccggct gtcgttctat ttgctggatc
    11941 agattaaacg gccgcccggg cagatcgaac ggcgcccggc tcagtaagcc ggcttcgagg
    12001 ctcg
//
LOCUS       DASWOG010000300         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_300479,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000300 DASWOG010000000
VERSION     DASWOG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300479"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..381)
                     /locus_tag="VGB93_06200"
     CDS             complement(232..381)
                     /locus_tag="VGB93_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145731.1"
                     /translation="MSFKIGGAQLLGFMRDYKIESRRCVISLPDEGATVEIFEGHVTR
                     LNDGL"
     gene            1144..1842
                     /locus_tag="VGB93_06205"
     CDS             1144..1842
                     /locus_tag="VGB93_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0145732.1"
                     /translation="MIVLYSYPTLFGVADNNGYGLKVFAFLRLVGLPFRHEHVFDASQ
                     APRGQLPYIVDDGETIGDSNTIITHLISKYRLTVDSALNARERDLDLLVVRLLDDLYW
                     VMSYSRWKDDRFWPIFSAALMREHPQLTDDDILKAREFNSKRYYFQGIGRYEPDAAYA
                     RGLADLGVLANLIKLNDYLHGAKPTSVDAAIYGFIANIHFYDIDTPLKEFVSARPVLV
                     RHCEAIHEIVMRAR"
     gene            complement(1904..2548)
                     /locus_tag="VGB93_06210"
     CDS             complement(1904..2548)
                     /locus_tag="VGB93_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145733.1"
                     /translation="MRAARSVFVALSVSALTIFMNGTPAAAQQRSGANGQLISNNQAA
                     PPQCDANCVRANAAKAADACAPRIETQAPSSFNWMSRPNAGIFQQADPSSPGDTIVRY
                     RGDSIRFLTADKSWLRVSYECAYDVRAGVVQFVKVRSGSLDQPLGPPSVQTASQHSAV
                     QAAALAAAIRAAVPGAAITSSALVKPKKRVHVWEPSQVEIQQQNPNPRLPAHST"
     gene            complement(2632..3063)
                     /gene="csgH"
                     /locus_tag="VGB93_06215"
     CDS             complement(2632..3063)
                     /gene="csgH"
                     /locus_tag="VGB93_06215"
                     /inference="COORDINATES: protein motif:HMM:NF041112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli-like amyloid fiber formation chaperone
                     CsgH"
                     /protein_id="HEY0145734.1"
                     /translation="MRVLQSPIPKPHVSRPTLIGVALAFIAAPLAAAPTGGDVLAQAA
                     SGPLHCEIQKSQSGDMVRLVGIATGTQAIAGTSRFVMIKSGTSGSSNMAQSQHFVLKA
                     QQQAVVGQATTNLDPGGHIAIDFGVRTDGGLVCNVRASLER"
BASE COUNT          679 a          964 c          929 g          717 t
ORIGIN      
        1 cgtcacatcc caaactccat cgcgaccatg cgacgacgat taacggtcgc gctcgccaga
       61 caactcgcgc gatgtccctg ctgtgcacga ccgatcaaga aagtcaggct acgaaattta
      121 tgacgcagta agactagggc cgttggtata agagaaacgt ctggcattgt gcggattgaa
      181 aaaaggctcg cgaagcgagc cttaatcgcg tgatttggcg ctcggccacc atcaaaggcc
      241 gtcgttgagc cgtgtaacgt gtccctcaaa aatctcaacg gtggcgccct cgtccggcag
      301 cgagatgaca cagcgccgcg actcgatttt gtagtcacgc atgaagccta aaagctgcgc
      361 gcccccgatt ttgaatgaca ccgctggtgc agccaccagg cgccaccgcc agaaaacact
      421 gactagcaaa ctgcaatcaa attctgttca actctctcgc ctgtcacaat attgtggccg
      481 ccgccaagcc aacttcaaat gcgaaattac tatagggggg cttcatgcgc ggcaagtcgg
      541 caagcagact gcttttgagt attgcgatga ccatatgcgg ggtcaccacg cccgcacttg
      601 ctgcacccaa ttgcaacagt ttcttttcga acgcggatgg ctcgtggagt ccgacgcatc
      661 caatcgttat tgccggaccg gcctcacaga cggtgatcac gcccagcgac aaatttcagt
      721 catggatgcc ggggttaccc gggcgcatcg ctagatcact caatgtgcat tgccgctacc
      781 aacgagtgca agtaggcgtg cacaacattc ctagaatacc gtgaaagccc gcgcaaagca
      841 ggcctttgtt ggttagccgg tgctggcgtc ggtgctaggc agcggctttc agggcggggt
      901 agacgtggca tgaatcaacc tcgatcagcg tgcctggcga ctcctcgatt tcgatcgcga
      961 ccgtctgtgt tggcctgggc atcatgattt ggagtctgat cgcgatattc agataactca
     1021 cgtttggccg agccagcgtt gtttcgtgcg atggccatgc gtttgtttgc cacttgccat
     1081 caaagctcag gcggtgattc actgcaccgc agcgccacaa gcgctcaccg gaggcactgc
     1141 attttgatcg ttctctattc ctatccaaca ttgttcggcg tcgccgacaa taacggatac
     1201 gggctgaagg tctttgcctt tttgcggctt gtaggtttgc ccttcaggca tgaacatgtt
     1261 ttcgatgcat cacaggcacc gcgcgggcaa ctgccctaca ttgtcgatga tggcgagacc
     1321 atcggcgaca gcaacaccat tataactcat cttatttcga aatatcgcct gacggtcgat
     1381 tcagcactca atgcccgtga gcgggacttg gaccttctcg tcgttcgtct gttagacgat
     1441 ctctattggg taatgtctta ttcgcgctgg aaagacgatc gcttctggcc gatattcagc
     1501 gcggccctta tgcgcgaaca tccgcagcta accgacgatg acatcctcaa ggcgcgcgaa
     1561 ttcaattcga agaggtatta tttccaaggc atcggtcgat atgagccgga cgcggcctat
     1621 gcccgtggtc tcgccgatct cggtgtgttg gccaatctaa taaagctcaa cgattatttg
     1681 catggggcca agccaaccag tgtggatgcc gcaatttatg gtttcattgc caacatccat
     1741 ttctatgaca tcgacacgcc cttgaaagaa tttgtttcgg cgaggccagt tctggtgcgc
     1801 cattgcgagg caatccacga aatcgttatg cgcgcccgct aatgcgcagc gggttcgcat
     1861 gctcgctcat ccgtcgacgg cttagaataa cccgacgctg cgcctaggtt gaatgcgctg
     1921 gcagcctcgg attggggttc tgttgctgaa tttcgacctg gctcggctcc cagacatgta
     1981 cccgtttttt cggcttgacc aacgccgaac tggtgatcgc cgcacccggc accgcggcgc
     2041 gaattgcggc cgcgagcgcc gcggcctgaa ccgcggaatg ttggcttgct gtttggacac
     2101 tcggcggccc caaaggctgg tcgagactgc cagagcgcac cttgacgaac tgcacgacac
     2161 ctgcgcggac atcgtaggcg cattcgtaac tcactcgtag ccagcttttg tccgccgtca
     2221 gaaaccggat cgaatcgccg cgatagcgga cgattgtgtc gcccggcgaa gaggggtccg
     2281 cttgctggaa aatccccgcg ttaggccggg acatccagtt gaaactgctc ggcgcctgcg
     2341 tctcgatgcg gggcgcgcag gcgtcagccg ctttggcggc attggcacgc acgcaattgg
     2401 catcacattg cggtggcgcc gcctgattgt tcgagatcaa ttgcccgttc gccccgcttc
     2461 gctgctgcgc ggccgcgggc gtgccattca taaaaatcgt cagcgcggaa accgagagcg
     2521 cgacgaagac ggatcgggca gctcgcaagg gttcgaattg caacgcttgg tactctcacg
     2581 agattgaaat ttggtccgcg acgcggagcc cgagcttcca aacacagttt tttaacgttc
     2641 caatgacgcc cggacgttgc acacgagtcc gccgtccgtc ctgacgccga aatcgatagc
     2701 gatgtggccg ccggggtcga gatttgtcgt ggcttggccg accacggcct gctgctgcgc
     2761 cttgagcacg aaatgctggc tttgggccat attcgatgat cccgaggtgc cggatttaat
     2821 catgacgaaa cgcgacgttc cagcaattgc ctgcgtgcct gtcgcgatcc cgacgagtct
     2881 gaccatatcg ccgctctggc ttttttgaat ttcacaatgc agcggtcccg aagccgcctg
     2941 cgcgagcaca tcgccgccag tcggcgcggc cgcaagcggc gcggcgataa acgcaagtgc
     3001 gaccccgatc aatgtgggcc gcgaaacgtg aggcttcggg atcggcgatt gcagaacgcg
     3061 cacaggcttc tcctttgcca attgccccaa tatcggacgg cggaccaatg cgccacgaag
     3121 aaaaagcggg gcgccgaggc gccccactct cttgagctta cttgccctgc gtgatcgaga
     3181 cgtggttgcc cgcgcccgtc tgcgaataat aggcatagtt gaagttgccg gactgagcga
     3241 ccgtcgcggc gtcgccgctg ccggactgcg ttccgtagat cgtgtcgtt
//
LOCUS       DASWOG010000301         9705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_190449,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000301 DASWOG010000000
VERSION     DASWOG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190449"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..77
                     /locus_tag="VGB93_06220"
     tRNA            4..77
                     /locus_tag="VGB93_06220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Gly,seq:tcc)
     gene            392..712
                     /locus_tag="VGB93_06225"
     CDS             392..712
                     /locus_tag="VGB93_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0145735.1"
                     /translation="MIVATTNDIAGYRIVRHLGLVRGITVRSRSVVGNFVGGLQSFFG
                     GQISAFVHLAETARQEAFDHMCEHARQGGANAIVGMRYDANEITDGITEVLAYGTAVW
                     VEPV"
     gene            complement(709..1143)
                     /locus_tag="VGB93_06230"
     CDS             complement(709..1143)
                     /locus_tag="VGB93_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145736.1"
                     /translation="MTKLRGFGQLSLRAASAWLASGLSTAVLAQSSLPAPETTQVSNG
                     DMADTVNDPRIEAARKSGMGTWKWAVPPVKMKGGFEDHDPIGLAAGKLVPADCSINWT
                     DPDTHKLYCFTSATSLVYFLDAPHTNLARAEKGWRALKGPSS"
     gene            1344..1419
                     /locus_tag="VGB93_06235"
     tRNA            1344..1419
                     /locus_tag="VGB93_06235"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1377..1379,aa:Trp,seq:cca)
     gene            complement(1501..2301)
                     /locus_tag="VGB93_06240"
     CDS             complement(1501..2301)
                     /locus_tag="VGB93_06240"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145737.1"
                     /translation="MGKTILITGASSGIGRATALYFAAKGWNVAATMRDPLKADPALK
                     HPQISLFALDVTNEDSIARALADSLTRYIKIDVLLNNAGYALFGPIEAADGRQIQQQF
                     ATNLFGLIDVTQQILPVMREAGEGLIINISSIIGRMALPYAASYIATKFAVEGLSESM
                     RYELEPFHIRVKLIEPGSIRTEFGKGSMQTAISDPYRASMTKFLAVFSKSNSRGSTPE
                     SVAKIIYSAANDPSNRLRYLAKPGPFFWLNQLLPDFLWRRVLVKAMVK"
     gene            2389..2814
                     /locus_tag="VGB93_06245"
     CDS             2389..2814
                     /locus_tag="VGB93_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0145738.1"
                     /translation="MNSWDLLQLHHQFFERITQNAERALADDKLDVKGFFVLASIEEL
                     KHPAALAKRLVLPKPTVTFLIKKLEQAQYVKRKTVAGDLRRFEIQLTPLGRKVCERGR
                     KTLSAAFDISLSRLSSSEREQYSELLRKCVQPAEAGVQI"
     gene            3138..3722
                     /locus_tag="VGB93_06250"
     CDS             3138..3722
                     /locus_tag="VGB93_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145739.1"
                     /translation="MRKMVAAWAVLALAGEIFCLSQVPAWAENSACRGLVDAMVKNSK
                     TPYHSVGTISFDPKDPPAPGTTISPKPISTESIFTGTQVFAKTPNGQWKDIHAVISDL
                     QQRVADSVESFTDCRRAPDEMIDGKRFVVYIGSQDSGALNIATKIWVSVETGTLALSD
                     IMITAPTPPDGKIRHQLMSLRYDYAGIKPPASSN"
     gene            complement(3737..5050)
                     /locus_tag="VGB93_06255"
     CDS             complement(3737..5050)
                     /locus_tag="VGB93_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145740.1"
                     /translation="MADDRPTGRQSFTPSSFIYSPTTHRLRFIAVALLVGAGCINYLD
                     RAAVAVAEPQVHQELHLSYGQVGVLLSAFAWSYGLAQIPAGTLLDRFGARRTLGVGLV
                     LWSLAQLSATFVRSLGQFIVARVALGLGESPMYIGGTRVCTDWFALDQRALPIAIFNS
                     SSGLAPALAPPLLTWLMLAFGWRQMFLIAGLAGFAVTALWVIFYRAPENADVPAADLA
                     EIRRQDSADVPHIGFRQVLWLLRFPTTWGMFLGFFGVVYISWLYATWLPAYLESSRHQ
                     SVASAGFWAAVPLAAGFFGALGGGFISEGLIRRGISPAAACRWPTVAGLLAAGLFTIA
                     ASQSGNVYVAIGLIACGLFAANVASSCGWALPAVIAPPNVVATLEAVQNVGGSVGGAL
                     APFITGTLIQAKGSFVPAFILAGIISLACALIYLFGTRGKIVASV"
     gene            5142..5732
                     /locus_tag="VGB93_06260"
     CDS             5142..5732
                     /locus_tag="VGB93_06260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0145741.1"
                     /translation="MPDRILVIYGSYRVDRLGIRLANYLVRSLSQRGAEAELIDAKAI
                     GLPMLDRMYKEYPKGTAPAAMEALAENIRRADAFIFVTGEYNWGPQPGLKNLTDHFLE
                     EWFWRPAAVASYSAGRFSGVRSGTVWHGILSEMGMVVVSSTLAAGPITQALDANAQPV
                     GEGGKALEQSFPRFADDLAWWTEAAREQRARRAPPY"
     gene            5742..6050
                     /locus_tag="VGB93_06265"
     CDS             5742..6050
                     /locus_tag="VGB93_06265"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY0145742.1"
                     /translation="MNDLLPANPSTDQLARIIGNVAAPAFLLGAVASFISVLISRISH
                     IIERSQYLHSIADDDAAKTYLKTDIPRLKRRAALYKSIAVLRHRSGDTYRAHYHCRLR
                     "
     gene            6034..6186
                     /locus_tag="VGB93_06270"
     CDS             6034..6186
                     /locus_tag="VGB93_06270"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145743.1"
                     /translation="MVAFVSAFYRVAHEYGVAFLFVAALITFCVSLIDLARETRIALR
                     DNDLHK"
     gene            complement(6264..7256)
                     /gene="egtD"
                     /locus_tag="VGB93_06275"
                     /pseudo
     CDS             complement(6264..7256)
                     /gene="egtD"
                     /locus_tag="VGB93_06275"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
     gene            complement(7217..8494)
                     /gene="egtB"
                     /locus_tag="VGB93_06280"
     CDS             complement(7217..8494)
                     /gene="egtB"
                     /locus_tag="VGB93_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY0145744.1"
                     /translation="MTAALFRSHLKTDQLLSLYQAVRATSRGLGARLSDADATPQSMP
                     DASPAKWHLAHTTWFFEKMVLVPFLPDYRCFDETYNFLFNSYYNSIGARQPRPKRGLI
                     TRPSLDDVYRYREHVDAGIARLLEKSTPEAATELIELGCHHEQQHQELLLTDILHLFA
                     QNPLHPAYRSAEPLAVESNGADALQYLSLPDGLIEVGNDGEGFSFDCEGPRHKTFIEP
                     FRLADRLVTNKEWIEFIADGGYRNPLLWLSEGWAKTQAEGWTTPLYWDERGDANYWTM
                     TLRGAQPVDPAAPVTHISYFEADAFATWAGRRLPTEFEWEYAARGVPQNGNLLDTDRL
                     RPRPAPARTGGLRQMYGDVWEWSRSTFSPYPRFRTAEGAVGEYNGKFMCGQFVLRGGS
                     CGTPASHIRATYRNFFPPVARWQFSGLRLAEDA"
     gene            8697..9296
                     /locus_tag="VGB93_06285"
     CDS             8697..9296
                     /locus_tag="VGB93_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0145745.1"
                     /translation="MRRAARAVSQAVATKVTDFASVSPIVGAGIVIALGGFCAAAMAD
                     TKPSQQTPSPPPVALAASAPKSAPAPAPAKDAVPAVVLDDKDIEGVMGREIYSIAGED
                     MGRIVDILVDHKGNLRAAIIDFGGFLGVGSRKIAVDWHAIQFGQDRALLSLTRDQVRV
                     APEYKDGEPVVILGPGSSVPPQRVSARQQQKPPVTAPTK"
     gene            9370..>9705
                     /locus_tag="VGB93_06290"
     CDS             9370..>9705
                     /locus_tag="VGB93_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145746.1"
                     /translation="MTQFPDIKANATGPHPSRRSLVGLDWFTFFLADVQTGFGPFVAV
                     YLTTAQWTQQDIGFILSITGIIGLIGRIPGGAIVDAARSERNIAAIAVVCIGISAPML
                     AAWPLFPLVF"
BASE COUNT         2028 a         2775 c         2927 g         1975 t
ORIGIN      
        1 cgcgcgggtg tagctcaatg gtagagcagc agccttccaa gctgaatacg agggttcgat
       61 tcccttcacc cgctccaaat ttgggaaagt ttatgaaaat cacattgttg gctgcgattg
      121 ccgcgattgc gctcagcctg agtggtactt cggacgtgtt tgccgcgcct gttgacggcg
      181 ccgcgatctc aggtgcatcc agtttttccg cgccgatcag caaggcccat tggtgccggg
      241 tccatcgctg gtgcgaacac ggccactgct gggtgcatcg ccactgctgg tgacggaaga
      301 gacgctatgt tgcgaaccgg gggccgcgcg atgagcgcgt tctccgggat ttatttgata
      361 ttcaaaaaca tttccgacgc gggagagggc catgatcgtc gcgacgacca atgacatcgc
      421 cggctataga atcgtccgtc atctcggcct tgttcgcggt atcactgtgc gctcgcgcag
      481 cgtggtcggc aattttgtcg gcggcttgca atccttcttc ggtggccaga tcagcgcctt
      541 cgttcatctt gccgagaccg cccggcaaga agccttcgat catatgtgcg aacatgcgag
      601 gcagggcggt gccaatgcca tcgtcggcat gcgttacgat gctaacgaaa tcaccgatgg
      661 gatcaccgag gtcctggcgt acggcactgc ggtctgggtc gagccggtct agctcgacgg
      721 ccctttcagc gcgcgccagc ccttttccgc ccgcgccagg ttcgtgtgcg gcgcgtcaag
      781 gaagtacacg agcgatgttg cgctcgtgaa gcaatagagc ttgtgcgtgt cgggatcggt
      841 ccagttgatc gaacagtctg caggaactaa tttgcccgcc gccaagccga ttgggtcgtg
      901 gtcctcgaag ccgcccttca tcttcactgg cggcacggcc catttccagg tgcccatgcc
      961 cgactttcgc gcggcctcga tccgagggtc gttcacggtg tcggccatgt ccccgttcga
     1021 gacttgggtg gtttcaggag cgggcaggct gctttgcgcc aaaacggcgg tgctcaagcc
     1081 gctcgcgagc cacgcgctgg ctgcgcggag cgataattgt ccgaaacccc gcaatttcgt
     1141 catgagtgcc tccgccgatc gacgacccta acataggccg ctcgcgcgga taacgcatca
     1201 caatcgctga gggttttcgg cggcggtcgc aaaaatcaat ggtgtcttaa cgaccgccta
     1261 acatcggcgg ctgaaacagc cgcgacccta ctcatgccgt ttcgcttgct ttggcctcgc
     1321 agctgcggca aatacggggc gctaggagtg tagctcaatt ggtagagcac cggtctccaa
     1381 aaccgggggc tgggggttcg agtccctcct ctcctgccag aatctgataa agcactttaa
     1441 aatcaataaa ttaatggccg ccggattctt tattttcaaa tagggcggct tgatcttttt
     1501 ctatttgacc atcgctttca ccaaaacccg ccgccacagg aagtcgggca aaagttgatt
     1561 caaccaaaag aaggggccgg gtttggccag gtagcggagc cgattgctgg ggtcatttgc
     1621 tgcgctgtag attattttcg caaccgactc cggggtggac cctcgagagt tcgatttgga
     1681 aaatacagcc aaaaatttgg tcatgctggc tcgataaggg tcgcttatcg ctgtttgcat
     1741 gctccccttg ccgaactcgg tcctgatgct gccgggttcg attaatttca cgcggatatg
     1801 aaacggctcc aactcataac gcatggattc gctcaatcct tcgacggcga attttgtggc
     1861 gatgtacgac gcggcgtagg ggagcgccat acggccaata atggaagaaa tattgataat
     1921 caaaccttcg cccgcttccc gcatgacggg gagaatttgt tgcgtgacat cgattaagcc
     1981 gaatagattc gtcgcaaatt gctgttggat ttggcggccg tcggccgctt cgattgggcc
     2041 gaacaaggcg tagcctgcat tgttcagcag cacgtcgatc tttatataac gagtcaacga
     2101 atcggccagg gctcgcgcaa ttgaatcttc attcgtcaca tccaacgcga acaagctgat
     2161 ttgtggatgc ttgagcgcgg gatcggcctt gagcgggtcg cgcatggttg cggcgacatt
     2221 ccagcctttt gcagcgaaat agagcgcggt ggcccgtccg ataccgctgg aggctcctgt
     2281 tatcaagatt gttttaccca atggtaacga tcctccagtt aggttacaat actattccac
     2341 atggaatagt tccatatcaa actaaatgat cgggccgtca aggaaaatat gaacagttgg
     2401 gaccttctgc agctgcacca tcagtttttc gaacgcatca cacagaacgc ggagcgcgcg
     2461 ctggcggatg acaagttgga cgtcaaaggc ttttttgtgc tggcctcaat tgaagaactg
     2521 aagcatccgg ctgctcttgc caaacgtctg gtcttgccca agccgacggt gacttttctg
     2581 atcaagaaac tggaacaagc ccagtatgtg aaaagaaaaa ctgtagcggg cgatttgcgg
     2641 cgatttgaaa tccagctcac accattggga cgcaaagtgt gcgagcgggg ccgcaaaact
     2701 cttagcgccg cctttgacat aagtctcagc cggctcagct caagcgaaag agagcaatac
     2761 agcgaactct tgaggaaatg cgtgcaaccg gccgaggcgg gagtccagat ttagccgcgt
     2821 cagaacaaag cctgcccgat ttggcggtcc agcggacttc ctaggccatt gaaaggcaat
     2881 ctggcgccaa ggcgaactga gagttcttgg acaagccgtt ttgtaagctc ttgccattgc
     2941 ttgacccgtt ttcattcaat gaaattgcct aagtcattgt ttgaaataga gaaatagaga
     3001 atggtggctc ctcctctcct gccatctgaa ttttttagct ataaaaaaca aagaattgcg
     3061 tgggttgaac tctctcgacc gggcggtgcg cgggcagcta tgagtcctct gcaaccgggg
     3121 caaaagaagg atgcggaatg cgtaagatgg tggcggcgtg ggcggtgctg gctctggcgg
     3181 gtgagatttt ctgtctcagc caggtgccgg cgtgggcgga gaactccgct tgccggggcc
     3241 ttgttgatgc gatggtcaag aattccaaga cgccttacca cagcgtcggc acaatctcgt
     3301 tcgatcccaa ggatccgcca gcgcctggca cgacgatctc accaaagcca atatcgaccg
     3361 aatcgatttt caccggcaca caggtttttg caaaaacgcc gaacggacaa tggaaagata
     3421 tccatgccgt gatctcagat ttgcagcagc gggttgcaga cagcgtcgag agttttacag
     3481 attgcaggcg ggcacccgat gaaatgatcg acggcaagcg attcgtcgtc tacatcggct
     3541 ctcaagacag cggcgcgctc aatatcgcga ccaaaatttg ggtgtcggtc gaaacgggca
     3601 cgctggccct ctccgatatt atgattacgg cacctacgcc tccggacggc aagatcaggc
     3661 atcaattgat gtcgttgcgc tacgactatg ccggcatcaa acccccggcg tcatcgaact
     3721 agtcgatcga gcagcctcat accgaggcca ctattttccc gcgtgtaccg aaaagataga
     3781 tcagcgcgca ggcaagtgaa atgattccgg cgaggataaa ggccgggacg aacgatcctt
     3841 tggcctggat cagcgtgccc gttatgaaag gcgccagtgc gccgcccacc gaaccaccga
     3901 cattttgaac ggcttcgagc gtcgcgacaa cgtttggcgg cgcgatcacc gccggcaagg
     3961 cccatccgca acttgaggcg acgttcgcag cgaaaagccc gcaggcaatc aggccaattg
     4021 caacatagac attgccgctc tgcgacgccg cgattgtgaa tagtccggct gccagcaggc
     4081 ccgcgacggt gggccaccgg caggctgctg ccgggctgat tccacgccgt atgagaccct
     4141 cggatatgaa gccgccgccg agggcgccga aaaagcccgc ggcaagcggg accgccgccc
     4201 aaaacccggc gctggcaacg ctctgatggc gcgaactttc cagataggcc ggcaaccacg
     4261 tcgcatagag ccaggaaata tagacgacgc cgaaaaagcc gaggaacatg ccccacgtcg
     4321 tcggaaagcg caggagccat aagacttgcc gaaagccgat gtgcggaacg tcagccgagt
     4381 cctgccggcg gatttccgcg agatcggccg cgggcacgtc cgcattctcc ggcgcgcggt
     4441 agaagataac ccagagagcc gtaactgcga atccggcgag gcctgcgatc agaaacatct
     4501 ggcgccagcc gaaagccagc atcagccacg tcagcagtgg tggcgccaac gccggtgcga
     4561 gccccgagga cgaattgaaa atggcgatcg gcaatgcgcg ctggtcgagc gcaaaccagt
     4621 ccgtgcagac ccgcgtgcca ccgatataca ttggggattc gccgaggccc aatgcgacgc
     4681 gcgcgacgat gaattgaccg agcgagcgca cgaaggtcgc gctgagctga gccagtgacc
     4741 ataagaccag gccgacgccc aatgtccggc gcgctccgaa gcgatcaagg agagtgcccg
     4801 ccggaatttg tgccaggcca taactccagg cgaaggccga gagcagcacg ccaacctggc
     4861 cgtaagaaag gtgaagctcc tgatgaactt gcggctcggc gaccgcgacc gcggcgcgat
     4921 cgaggtaatt gatgcatccg gcgccgacga gcagggccac cgcaatgaag cgcaggcgat
     4981 gtgtcgtcgg cgaatagatg aaagaggaag gcgtgaagct ctggcgtccg gtagggcgat
     5041 cgtctgccat gttatgcggg tgcaatgggc gaggcggatg ataaggtctt tttcattgaa
     5101 cgccccttca tgaataatgc gagctggaag cgagaatgaa tatgcccgac cgtatactcg
     5161 taatttacgg atcgtatcga gtcgaccgtc tgggaatacg gctcgcgaac taccttgtgc
     5221 ggagcctcag tcagcggggt gcggaagcgg aactcatcga tgccaaagcg atcggcctgc
     5281 cgatgctcga tcgcatgtac aaggaatatc caaaaggcac ggcgccggcg gcgatggaag
     5341 cgctggctga aaatatccgc cgcgccgacg ccttcatctt cgtaaccggc gaatacaatt
     5401 gggggccgca accgggcctc aaaaacctga ccgatcattt tctggaagaa tggttttggc
     5461 gcccggccgc ggtggcaagt tattccgccg ggcgcttctc gggcgttcgc tcgggaacgg
     5521 tctggcacgg cattctttcg gaaatgggta tggtcgtcgt gtcgagcacg ctcgccgccg
     5581 gcccgatcac gcaggcgctc gacgctaatg cgcagccggt cggtgagggc ggcaaggcgc
     5641 tcgaacagag cttcccgcgt tttgccgacg acctcgcatg gtggacggag gctgcgcgcg
     5701 agcagcgcgc gcgccgcgcg ccaccttact agtatatgca aatgaacgat ctgcttccgg
     5761 caaatccatc aaccgaccag cttgcccgca tcatcggcaa tgtcgccgca ccggcgttct
     5821 tgctgggcgc cgtcgcatca ttcatttcgg tactgatttc gcggatcagc cacataatcg
     5881 aacggtcgca atatttgcac agcatcgccg atgacgatgc cgccaagacc tatctcaaga
     5941 ccgacatccc ccgcctcaag cgccgggcgg ctctttataa atcgatcgct gttctgcgcc
     6001 atcgtagcgg cgatacttac cgcgctcatt atcattgtcg ccttcgttag cgcgttctac
     6061 cgcgtggcac acgaatatgg cgtggcgttc ttgttcgtag ctgcgctgat tacgttttgc
     6121 gtctcgctga ttgatctcgc ccgcgagacg cgaattgccc ttcgcgacaa tgatcttcac
     6181 aaatagacca tcgcggtacg aatgaatatc gagtgggaca agtagaagtg gtttttctcg
     6241 cttgcccctt cgcttgataa aattcacacg gcgctgaggc cgaagagcgc gaagcgctgc
     6301 tcggcgtccg tccagatcgc gctgacctgc cagccgcttt cgatcgcgag ttcggaaaag
     6361 ctctcgatgc tgtatttgcg cgaactttcc gtatggatcg tctcaccgtc gccgaaggcg
     6421 aatgagcagc cagcgaccgt gacattttgc tcttcgagac tgaccagatg catttcgatg
     6481 gcggattcgg cctcgttcca gcgcgcctca tgcgcgaacc gggcaagggc gaaattgccg
     6541 gagagttcgc ggttgatccg cgccagcagg ttgaggttga actcggccgt gacgccgtcg
     6601 cgatcgtcat aggcggcgat cagcgtcggg atatctttct tgagatcggc gccgatgatc
     6661 gcaaggccgc cggtgccgac gtggcggcgc atccgctgta gaaacgccag agcttcggcc
     6721 gtgtcgagat tgccgatcgt cgagccgggg aagaacgcga cgcggcgctt tgccggcaca
     6781 tgcgccggaa tgtcaaaatc ggaggtgaag tcggtcgcga ttggatggac gtcaagccga
     6841 ggaaagcggt cacgcatccg caccgcggtc tgatccagaa agttggccgc aatgtcgatc
     6901 gggacgtaaa gctgcggcgt ctccagcgcg ccgaggacga tctcggtctt cacgccggcg
     6961 cccgcgccat attcgagaag aatcgcgccg cggccgccaa gagcggcgat ccgccgggca
     7021 ttgtcgttga gaatcgcggt ctcggtccgc gttggatact attcatcgag ccgcgtgatc
     7081 gcctcgaaga gttcgcagcc gcgggcgtca tagagccagc gggccggcag tgtccgctgc
     7141 ggacgcgcga gccccgctaa cacgtcggtg cgaaaggcat cgagctggcg cgtttttgca
     7201 tcgacgagct ctagcatcag gcatcctccg ccaggcgtag gcccgaaaac tgccatctgg
     7261 caacgggcgg aaagaagttg cgataggtcg cgcgaatatg gctcgccggg gtgccgcacg
     7321 agccgccgcg cagcacgaat tggccgcaca tgaatttacc gttgtactcg cctaccgcgc
     7381 cttcggccgt ccggaagcgc gggtagggcg agaaggtgct gcggctccat tcccagacat
     7441 cgccgtacat ctgccgcagg ccgcccgtcc gcgctggcgc aggccgcggt cgcaaccggt
     7501 ccgtatcgag caggttgccg ttttgcggca cgccgcgcgc ggcgtattcc cactcgaatt
     7561 cggtcggaag ccggcgcccg gcccaggtcg cgaaggcgtc cgcctcgaaa tagctgatgt
     7621 gcgtgaccgg ggcggcggga tcgacgggct gcgcgccccg caatgtcatc gtccaataat
     7681 tggcatcgcc acgttcgtcc caatagagcg gcgtggtcca gccttcggct tgggttttcg
     7741 cccagccctc agaaagccag agcagcggat tgcgatagcc gccatcggcg atgaactcga
     7801 tccattcctt gttggtgacg aggcggtctg ccagccgaaa cggttcgatg aatgttttat
     7861 ggcgcggccc ttcgcaatcg aacgaaaagc cttcgccatc gttgccgacc tcgatgaggc
     7921 cgtcgggcag cgacaagtat tgcagagcat ctgcgccatt ggattcgacg gcgaggggct
     7981 cggcgcttct ataggctggg tgcaggggat tttgtgcaaa gagatgcaga atatcggtga
     8041 gcagaagctc ctgatgctgc tgctcatgat gacagccaag ctcgatgagc tctgtcgcgg
     8101 cttccggtgt cgatttttcg aggaggcgcg caatgccggc atcgacatgt tcgcgatagc
     8161 gataaacatc gtcgaggctg gggcgggtga tcagaccgcg ctttggccgt ggctgtcgcg
     8221 cgccaatcga attgtagtag gaattgaaga ggaaattgta ggtctcgtcg aaacatcgat
     8281 agtccggcag aaacgggacc agcaccatct tctcgaaaaa ccatgtcgta tgcgccagat
     8341 gccattttgc cggactcgcg tccggcatgg attgaggcgt ggcatccgca tcagataatc
     8401 gagccccgag acctcggctc gtcgcgcgta cagcctgata aagagaaagg agctggtcgg
     8461 tcttcagatg cgaccggaat agggcagccg tcattcctcg tgctcctccg ttctttgccg
     8521 ctatcatttg gtgtggccaa ggcgaaaggc aagcttttcc gacgcaggac cggtttcggg
     8581 caaaaatcct tctctcgcgg gaaccttatc gcggggcggc gaattacacc ctgcttaact
     8641 tccgataatg cgccgtggcg tgtcgatgga agcttggcta gctcggggga gatcgtatgc
     8701 gccgtgctgc ccgcgccgta tcgcaggccg ttgccactaa agttacggat tttgccagcg
     8761 tgtcgcctat cgtcggggcc ggcatcgtca tcgccctcgg tgggttctgc gccgccgcaa
     8821 tggccgatac aaagccatcg cagcagacgc cgtcgccacc accagttgcg ttggcagcgt
     8881 cggccccgaa atcggctccg gcacctgcgc ccgccaagga tgccgtccct gcggtggtgc
     8941 tcgacgataa agacatcgaa ggcgtgatgg gccgcgaaat ttacagtatc gcgggcgagg
     9001 acatgggacg gatcgtcgat atcctcgttg accataaagg aaatcttcgt gccgcaatca
     9061 tcgactttgg gggatttctt ggtgtcggca gccgcaagat cgcggtcgat tggcacgcaa
     9121 tccagtttgg gcaggatcgg gcactcctgt cgttgactcg cgatcaggtt cgcgtggcgc
     9181 cggaatataa ggatggggaa ccggttgtca ttttaggacc aggctcgtcg gtgccgccgc
     9241 agcgggtatc ggcgcgtcag cagcaaaaac caccggtgac ggcgccgaca aaatgatcaa
     9301 ctgaacaaag ccatttcgcc gtgctgaatg accgccggaa tttttgctgt tgtccgaacg
     9361 agaaggtcag tgacgcaatt tccggatatc aaggcgaatg cgacgggccc ccatccgtcg
     9421 cgccggagcc tcgtcggtct cgactggttt acatttttcc tcgctgacgt ccagacgggc
     9481 ttcgggccat tcgtcgcggt ctatctgacc accgcgcaat ggacgcagca ggatatcggt
     9541 ttcatcctgt cgatcaccgg aattatcgga ctgatcggcc gaataccggg cggcgctatc
     9601 gtcgatgccg cgcgctccga gcgtaacatc gccgccatcg cggttgtctg tatcggcatc
     9661 agcgcgccga tgcttgcggc gtggccgctt tttccgttgg tcttt
//
LOCUS       DASWOG010000302         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_208199,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000302 DASWOG010000000
VERSION     DASWOG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208199"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>1691)
                     /locus_tag="VGB93_06295"
     CDS             complement(33..>1691)
                     /locus_tag="VGB93_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145747.1"
                     /translation="DTALNRVRCVLWDCDLARGHVYWSDSMYDMLGLKRERAFLSFGE
                     INALVHPHDGDLAHVAEMLAASQAKAIDHSFRMRHAKGHWIWLRARAEIVREGAGEAA
                     HLVGIAVDTSEQKILAERTATANMRLRDALETVSEAFVLWDRENRLVMCNSKFQRFHN
                     LPDTAVISGQPYQQVMAQGTSPLVQSQFALGERPSIGARTYEAQLGDGRWLQIDERRT
                     KDGGYVSIGTDITALKRHESQLLDSERRLTATVADLHKSRQTLEHQAQQLAELAEKYL
                     EQKAEAESASRAKSEFLANMSHELRTPLNAIIGFSDMMIGEVFGALPARYLDYSKDIQ
                     SSGQQLLALISDVLEMSRLDAGRVKLQKAEVAIGTIVSAALQGIEAAAREKSIALTSQ
                     IAATARLHADSAALERVIAALLHNAVKFTPEHGSVNIRARHVNGALNVYVEDNGVGIA
                     PAALERLGRPFEQWNAPLANGMKGSGLGLAIARSLVALHGGSIRIRSTPGHGTIVQVH
                     LPIEKPAFAVASKPAARRPLTSVQPHVAGRAISRPEIQVSRRPY"
BASE COUNT          316 a          493 c          530 g          352 t
ORIGIN      
        1 tttggaaacc gaagcgcggg taggcgcaat cttcaatacg gtcgtcggct gacttgtatt
       61 tctggccgcg atattgcgcg cccagccaca tgcggctgga cgctggtcag cgggcgccgg
      121 gccgccggct tggacgcgac agcgaaggcg ggtttttcga tgggaagatg gacctgcacg
      181 atcgtgccgt ggcccggagt cgagcggatg cggatcgacc cgccatgcaa cgcgacgagc
      241 gagcgggcga tggcgaggcc gaggcccgag cccttcatgc cgttggcgag cggtgcattc
      301 cattgctcga acggccggcc aagcctttcc aaagccgccg gcgctatacc caccccgttg
      361 tcctcgacat agacattcaa agcgccattg acgtgacgcg cgcgaatatt cacgctccca
      421 tgttcgggcg tgaatttcac cgcgttatgc aagagcgcgg cgataacccg ttcgagggcc
      481 gcggaatcgg catgaagtct ggcggtggcc gcgatctggc ttgttagcgc aatcgacttc
      541 tcgcgtgctg ccgcttcgat gccttgcagc gcggcggaca cgatcgtgcc gattgcgacc
      601 tcggctttct gcaatttgac acgcccggca tcgagccgcg acatttcaag cacatctgaa
      661 atcagtgcca gcaattgctg accgctgctt tggatatcct tggaataatc gagatagcgc
      721 gcgggcaggg cgccgaagac ttcgccgatc atcatgtccg agaacccgat gatcgcatta
      781 agcggcgtac gcagttcgtg gctcatgttg gcaagaaact cggatttcgc tcggcttgcg
      841 gattcggcct cggctttttg ttcgagatat ttctcggcaa gttctgcaag ctgctgcgcc
      901 tgatgctcga gcgtctgacg tgacttgtgc aaatccgcga ccgttgcggt gaggcgccgc
      961 tcggagtcga gcaattggct ttcgtgccgc ttgagggcgg tgatgtcggt gccgatcgaa
     1021 acgtagccgc catccttggt gcggcgttca tcgatctgca gccaccggcc gtcaccgagt
     1081 tgcgcttcgt aagtccgcgc gccgatagag ggtcgttcgc cgagggcgaa ttgcgattga
     1141 accagcggcg acgttccttg cgccatcacc tgttgataag gctgcccgct gatgacggcg
     1201 gtgtcgggaa gattgtgaaa tcgctggaac ttggaattgc acatgaccag ccgattttct
     1261 ctgtcccaaa gaacaaaggc ttcggagacg gtttccagtg catcccgcag ccgcatattt
     1321 gccgtggctg tccgttcggc gagaatcttt tgctctgatg tatcgacggc gatgccgacg
     1381 agatgcgcgg cttcgcccgc tccctcacgt acgatttcgg cgcgcgcccg cagccagatc
     1441 caatggccct tggcgtgccg catccggaac gaatgatcga tggctttggc ctgtgaggcg
     1501 gcgagcattt ccgcgacatg ggcgaggtcg ccatcatgcg ggtgaacgag cgcattgatc
     1561 tcgccaaagg agagaaatgc ccgctcccgt ttcaggccga gcatgtcgta catcgaatcc
     1621 gaccaataga cgtggccgcg ggcgagatca caatcccaaa gtacgcagcg gacacgattg
     1681 agggcggtat c
//
LOCUS       DASWOG010000303         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_25229,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000303 DASWOG010000000
VERSION     DASWOG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25229"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1861)
                     /locus_tag="VGB93_06300"
     CDS             complement(182..1861)
                     /locus_tag="VGB93_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY0145748.1"
                     /translation="MTSTPISPSPKERASLSTSRIVFLVIAAAAPLASMIGNLPIALG
                     LGNGAGTPGAFAIAGIILLCFAVGYAAMSRRIVATGAFYTYITEGLGTTLGVSSAYIA
                     LVAYLAFTFGLAAFFGYFTSLVLGQAGLHLPWLVYAVVGIFIVAVLGYRSIDLSSAIL
                     GALMIGEVIILAIFDIAVIHAKGFSLALPANAWSPHAVFTPGLGVSLMVAFTCFVGFE
                     SAALYGEEARDPKRSIPVATYTAVISIAIFYLFTAWVAVGAIEPDAIAVRANKETGEL
                     MFNIFTRYGGEVLTDIMGVLVCFSILASYLAIHNAAARYIFALGREWLLLPWFGEAHP
                     KSGAPHRASMTVSVMTLIAVIPFALTGLDPFKAGVPVLIGIGSFGIIFLQAFAAIAIL
                     VYLRRHGGEPSWVMLATWFGAIGLICATIFVGIYFKFLATSDATLVGLLPLLFPLVVI
                     PFAIFSVLLKFVRPKAPSAQDGALIDEAMSSPKIFTDAGPEFLIRDSAAGAAAWVIKY
                     KNVAGSEIMSGSKPSFEIALAEARNLRDQRGAKILELIGLPAHEGDGGGNV"
     gene            complement(2158..>2295)
                     /locus_tag="VGB93_06305"
     CDS             complement(2158..>2295)
                     /locus_tag="VGB93_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0145749.1"
                     /translation="AFGVPFIANPDLVERLRRNVPLNTPDKATFYGGDAHGYTDYPAL
                     T"
BASE COUNT          573 a          647 c          674 g          401 t
ORIGIN      
        1 ttccgcagat tgccttttcg gccgggatcg aagccttgct gcaattgagt ggcgcataag
       61 aggctgtctg gccgaggcag gacgcgggca tgatggccga gccaagcaga acgacgagaa
      121 cgacgagacg ttgatgcacg gccattatgc cctcccaagg caaatacaat attcagcggc
      181 cctaaacgtt tccgccgcca tccccctcat gcgcaggcag gccgataagc tccaatattt
      241 tagcgccgcg ttggtcgcga agattgcgcg cttcggccag cgcaatctcg aagctcggct
      301 tgctgccgct catgatctcg ctaccagcca cgtttttgta tttgatgacc cacgccgccg
      361 cgccggcggc actatcgcga attaaaaatt ccgggccggc atcggtgaaa attttcggcg
      421 aagacatggc ttcgtctata agcgcgccat cctgtgcaga tggcgctttg ggacgtacaa
      481 atttgagcag cacgctgaaa atggcgaagg ggatgacaac gagaggaaag aggagcggca
      541 gcaggccgac cagcgtcgca tcgctcgtcg ccaggaactt gaaatagatc ccaacaaaaa
      601 tcgtcgcgca gataagaccg atagctccga accacgtcgc aagcatcacc catgacggct
      661 cgcccccatg gcggcgcaga taaaccagga tggcaatcgc cgcaaaggct tgcaggaaga
      721 tgatgccaaa tgagccgatg ccgatcagaa ccggaacgcc ggccttgaac gggtcaagcc
      781 ccgtcaatgc aaatggtatg acggcgatga gcgtcatcac gctgacggtc atgctggcgc
      841 gatgaggtgc tccggatttt gggtgcgctt cgccaaacca gggcagcagc agccattccc
      901 ggccgagcgc aaagatataa cgcgccgccg cgttatggat cgccaaataa gaggcgagaa
      961 ttgaaaagca gaccaatacg cccatgatgt ccgtgaggac ttcaccgcca taacgcgtaa
     1021 agatgttgaa catcaattcg ccggtctctt tgttcgcacg gacggcgatg gcgtcgggct
     1081 cgatggcgcc gaccgcgacc catgcggtaa agagataaaa gatcgcgatg gatatgacgg
     1141 cggtataggt cgcgactgga atcgagcgtt ttggatcgcg cgcttcctcg ccgtaaagcg
     1201 cggcggattc gaagccgaca aagcatgtga acgcaaccat caggctgacg cccagacccg
     1261 gtgtgaacac ggcatgcgga ctccaggcgt ttgccggcag cgcgagagag aaacctttgg
     1321 cgtggatcac cgcgatatcg aagatcgcca aaatgatcac ttcgccgatc atcagggcgc
     1381 cgaggatagc ggacgacaaa tcaatcgatc tgtagcccaa cacggcaacg atgaagatgc
     1441 cgacaaccgc atagaccagc cacggcaaat gcagcccggc ctggccaaga accagactag
     1501 tgaaatatcc aaaaaaggct gccagaccga aggtgaaggc tagataggca acgagcgcga
     1561 tataggcgga acttacgccc agcgtcgtgc cgagcccttc ggtgatgtag gtgtagaagg
     1621 cgcccgtcgc gacaatccgg cggctcatcg ctgcatagcc gactgcgaaa cagagaagaa
     1681 tgatgcccgc gatcgcaaac gcgcccggcg ttcccgcgcc attgccgaga cccagcgcga
     1741 tcggaagatt gccgatcatc gatgcaagcg gggccgcagc cgcgatgacg aggaaaacga
     1801 tgcgggacgt cgagagcgaa gctcgctctt ttggcgatgg agaaatcggc gtgctggtca
     1861 ttcagtcttg atatgttgca aaggattcac cgcgatcttt aacgatcgaa tgcccttgct
     1921 gtatagaccg tattagggat tgacgacaat caatgcgcgc taggcgaggc cgccgccggt
     1981 aagcacgcgc ggttagcggt cctcccccgg gagtccaatc gggaagaccg ctaacacttc
     2041 ttagcattgc acgtacaacg ctattcgcca cggggcagta aacaattagc tggaacgtga
     2101 acgacacgac atgacaaaag acacacgcta gacagatggg tcatccatgc ttcgcattta
     2161 cgttaatgct gggtaatccg tgtagccgtg cgcgtccccg ccgtagaacg tcgccttgtc
     2221 gggcgtgttc aacggcacat tgcggcgcag ccgttcgacg agatctggat tcgcgatgaa
     2281 cggcacaccg aaagc
//
LOCUS       DASWOG010000304         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_98774,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000304 DASWOG010000000
VERSION     DASWOG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98774"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGB93_06310"
     CDS             <1..519
                     /locus_tag="VGB93_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY0145750.1"
                     /translation="HAREGANLSEAVERTKPDLIIIDMALPDRDALEDVRAVSADKPV
                     VMFADSDDPSFVEDAIAAGVCSYNLSGIAFQDVKPIMASAVALFRRYRRVEDELAAAK
                     TQLEERRAIERAKAILMKHRKMTEPEAYRWLQKKAMDENRKLPQVVTQFVREHDIESS
                     PKAEEKAGEKRS"
     gene            516..1739
                     /locus_tag="VGB93_06315"
     CDS             516..1739
                     /locus_tag="VGB93_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0145751.1"
                     /translation="MSDIVSLSAARKTATPKMRIGLLHLTDGAPAMVAHEFGFFAEEG
                     IETELFVEPSWANIADKLAFGFLDAAVVVPPLAFAVQMGLRGPVQPLLIPSAISSRGS
                     TITLNNALAQRTQERAARDNLSTVEALAAVLRARPATLGIVHAYSTHNLYLRYWLATA
                     GIEVGRDVKLMVVPPARAVEALTSGHVAGFCAGAPWGEVARRAGAGVTIATSHDVWAN
                     APEKAFAVRELWAEENPQALTTALRALLRAAKFCDAPENASYIAALLSRRRYFALDSH
                     AILASLPSGAMGDSACVFYRGAATFPWRSHGLWFLNEMRRWGLIDPTVDLRDLTTRVY
                     RPDLYRAAAQTMDIPVPTVDWKNEGAHDKPWMLEAAPEALAMPADRFCDGTVFEPEKV
                     PPTGDPTAKSALHKF"
     gene            2120..3427
                     /locus_tag="VGB93_06320"
     CDS             2120..3427
                     /locus_tag="VGB93_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145752.1"
                     /translation="MKTHKVEQLIIATVGFFWCFLMWFSTAAFSPSIAAHYHLSIKAL
                     GLLASSAIWMAPIGRVVAGWASDRFGAPRTFAVILVVCGLVSIASAFTQNYELLFVER
                     VFVAIAGVSFVVGIQHVAQWFNANEIGTAEGLYAGTGNVGAGMGALVLPRLFGTDYHS
                     AFLWLGIIALFIAAWYLIRGRAAKTVEQGAIARQRSDLRSTAFVWSRYIAVALMLAYA
                     MSFGLEVAMNAWLPGYFTRGYHAAILALGFTNIAGLQIAAGTFAAVESFCASLFRPFA
                     GFMSDLFQRKGWTPLPFIAKSLPYAPRLHWLGIALILITLAMGGLTFAGLTGSLHASV
                     LTLVALGVFISFGTGGTFALVPLLFPDRPGTAAGFIGGVSTAAGIVYPLIFAASTNIH
                     MGYLYVALYMFVPIILFYFWAARYERHPEEHGIFTSALAVEDA"
     gene            3467..3628
                     /locus_tag="VGB93_06325"
     CDS             3467..3628
                     /locus_tag="VGB93_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145753.1"
                     /translation="MHAPISSDTQEPYEPWYKYGVAFLSLEMVIAIAVSAYSLFMTFH
                     GIGGFPGKH"
     gene            3655..4074
                     /locus_tag="VGB93_06330"
     CDS             3655..4074
                     /locus_tag="VGB93_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEY0145754.1"
                     /translation="MTPEQIHLVQSSFEKVVPIADDAAALFYGRLFEIAPEVRPLFKG
                     DMADQGRKLMMTLGTVVRSLDRLDSIMPAVKALAVRHVEFGVRPRHYEPVGFALLWTL
                     QQGLGEAFTPETSEAWAKAYGLLSKAMIAAATAPSEV"
     gene            4085..>5008
                     /locus_tag="VGB93_06335"
     CDS             4085..>5008
                     /locus_tag="VGB93_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145755.1"
                     /translation="MYQDKKFPTSPSNEPLRLVIIGNGMAPGRMLEHLLEEAPDRYKI
                     TIFNAEPRVNYDRIMLSPVLSGEKEYEQIVIHGDGWYIKHNITLYKGHKIVAIDRDAK
                     TVTSDHGVTEGYDKLVIASGSTPIILPVPGNDLAGVLTFRDLDDVNAMMLAAQSRAKA
                     VVIGGGLLGLEAAAGLSSHGMDVTVLHLMPTLMERQLDSAAGYLLQQAVESRGIKVIT
                     KANTKAILGDGKVEAVELADGTIIPATLVVMAAGIRPNGALAKRAGLDVNRGILVDAH
                     RQTSGPDISAIGECAEVGGHTYGLVAPLYDMA"
BASE COUNT          894 a         1604 c         1493 g         1017 t
ORIGIN      
        1 cacgcccgcg aaggcgccaa tctcagcgaa gccgtcgaac gcacgaagcc ggacctcatc
       61 atcatagaca tggcactccc cgaccgcgac gcgcttgaag acgtgcgcgc ggtgtccgcc
      121 gacaagcccg tggtgatgtt cgccgacagt gacgacccga gttttgtcga agacgcgatt
      181 gccgccggcg tctgctccta taatctttca ggaatagcat ttcaggacgt gaagccgatt
      241 atggcttcgg cggtcgcact cttccgccgc tatcgccgcg tcgaggacga actcgccgcc
      301 gcgaaaacgc aactggaaga gcgccgcgcg atcgaacggg cgaaagccat tctgatgaag
      361 caccggaaga tgaccgaacc cgaggcctat cgctggctgc agaaaaaggc catggacgaa
      421 aatcgcaagc ttcctcaggt cgtgacgcaa ttcgtcagag agcacgacat cgagtcctcg
      481 cccaaggcag aagaaaaggc gggagagaaa cgatcatgag cgacattgtc tcgctgtcag
      541 cggcgcggaa aaccgcgaca ccgaaaatgc ggatcgggct cctccatctc accgacggcg
      601 cccctgcgat ggtggcgcat gaattcggct tcttcgccga agagggcatc gagaccgaac
      661 tctttgtcga accctcctgg gcgaatatcg ccgacaagct cgcgttcggc ttcctcgatg
      721 ccgcagtcgt cgtgccgccg ctcgctttcg ctgtgcagat ggggctgcgc ggccccgtgc
      781 agccgctcct gattccctcg gcgatcagtt cgcgcggcag tactatcacg ctcaacaatg
      841 cgctggcgca gcgaacgcag gagcgcgccg cgcgggataa tctttcgacg gtcgaggcgc
      901 ttgccgcggt cttgcgtgcg cggcctgcga cgctcggaat cgtccacgcc tattcaacgc
      961 acaatcttta tctgcgctat tggctcgcga ccgccggaat tgaagtcgga cgcgacgtga
     1021 aactgatggt cgtgccaccg gctcgtgctg ttgaggcctt gacctcgggc cacgttgccg
     1081 gcttttgcgc cggggcgccg tggggcgaag tggcgcgccg tgccggcgct ggcgtgacga
     1141 tcgcgacgtc gcacgacgtc tgggcgaacg cgccggaaaa agccttcgcc gtgcgcgaac
     1201 tctgggccga agagaacccg caagccctga ccaccgcgtt gcgcgcgctt ttgcgtgcgg
     1261 caaaattttg cgacgcgccg gagaatgcct cctacattgc cgcgctgctg tcgcgccgcc
     1321 gctatttcgc gctcgacagt cacgcgatcc ttgcctccct gcccagcggc gcaatgggcg
     1381 acagtgcgtg cgttttttat cgtggcgcgg cgacctttcc ctggcgttcg catggcctct
     1441 ggtttttgaa cgagatgcgc cgctgggggt tgattgatcc cactgtcgat ctgcgcgacc
     1501 tcaccacgcg cgtctaccgc cctgatcttt atcgcgcggc ggcgcagacg atggacatcc
     1561 ccgtcccgac cgtggattgg aaaaacgaag gcgcgcacga caagccgtgg atgctcgagg
     1621 ctgcgcccga ggcgcttgcg atgccggcag atcgtttctg cgacggcacc gtcttcgagc
     1681 ctgagaaagt gccgccgacg ggcgacccga cagccaagag tgcgctgcac aagttttgat
     1741 ccatattgtt cagcatctga gcgttttgct tcgcggcaag cgcaacagct cgcttggatt
     1801 ttgcgttttc gctctgcaat ggcgctggca cacctcttgc gatccgtttt ccgtcggccc
     1861 gagctttttg ccgccgtcag caaactatct aatggcggat agtttcgtct cagcatcgtt
     1921 tctcgcgagc taatggcggt tcgcattccg gatgaatccg gcagcgctaa gcgctgatgc
     1981 gtgccaggcc gatgaaaatc tatgtgttcg tagctcgtgt gctgtcgccc gtgaattttt
     2041 cgcgcgcgag cgccatgtgc tgctgcccgg tcagcaccat cgccgcattg cgcctcttgc
     2101 agagtgagga gcagagggga tgaagacgca taaagtcgag cagctcatta ttgcgaccgt
     2161 gggcttcttc tggtgctttc tgatgtggtt ttccaccgca gcattcagcc caagcattgc
     2221 cgcgcattac catctgagca tcaaggcgct cgggttgctc gcaagctccg cgatctggat
     2281 ggcgccaatc ggccgcgtgg tagccggatg ggcctccgat cgtttcggcg ccccgcgcac
     2341 attcgccgtg atccttgtcg tctgcggcct cgtctcgatc gcctcggcct tcacacaaaa
     2401 ctatgagctg ctcttcgtag aacgtgtctt cgtcgcgatc gccggcgttt cctttgttgt
     2461 cggcatccag catgtcgcgc aatggttcaa tgccaatgag atcggcaccg ctgaaggtct
     2521 ctatgcgggc acgggcaatg tcggcgccgg catgggcgcg ctggttctgc cgcgcctctt
     2581 cggcaccgat tatcattccg cctttctttg gctcggcatc atcgcgctgt tcatcgcggc
     2641 ctggtatctc atccgtggac gcgcggccaa gacagtcgaa cagggcgcca tcgcccgcca
     2701 gcgcagcgat ctgcgcagca cagcttttgt ctggagccgc tatatcgccg tcgcgctgat
     2761 gcttgcctat gcgatgtcgt ttggtctcga ggtcgcgatg aatgcctggc tgccgggcta
     2821 tttcacgcgt ggctaccacg cggcaatcct ggcgctgggc ttcaccaata tcgcgggctt
     2881 gcaaatcgct gcgggcacgt tcgcagcggt cgaatctttc tgcgcctcgc tgttccggcc
     2941 gttcgcgggc ttcatgtccg acctgttcca gcgtaagggc tggacgccgc tgccattcat
     3001 tgccaagtcg ctcccctatg cgccgcgcct gcattggctc ggcatcgcgc tgattctcat
     3061 cacactggcc atgggcggcc tgacattcgc cgggcttacc gggtcgctgc acgcgtccgt
     3121 actgacgctc gtcgcgcttg gcgtcttcat ctcgttcggc actggcggca cattcgcact
     3181 cgtgccgctg ctcttccccg accggcccgg cacggccgcg ggcttcatcg gcggcgtttc
     3241 gacggcggcc gggatcgtct atccgctcat cttcgcagcg agcacgaaca tccacatggg
     3301 ttacctctat gtcgccctct acatgttcgt gccgatcatc ctcttctact tctgggcggc
     3361 gcgctacgag cgtcaccccg aagagcacgg cattttcacg agcgcgctcg ccgtcgaaga
     3421 cgcgtgagcc ttatccgatc attctcacat gaactacgag gttcccatgc acgcgccaat
     3481 ctcctccgac acgcaagagc catatgagcc ctggtacaaa tacggcgtcg cctttctctc
     3541 acttgaaatg gtcatcgcta tcgccgtgag cgcctattcg ctcttcatga cgttccacgg
     3601 catcggtggt ttccccggaa agcactgacg cacgatagag atcggagaac gccgatgacg
     3661 cccgaacaaa tccatctcgt acaatcgagt ttcgagaagg tcgtgcctat cgcggacgac
     3721 gctgccgcct tgttctatgg gcggctgttc gaaatcgcgc ccgaggttcg cccgctgttc
     3781 aaaggcgaca tggcagacca gggccgcaaa ctgatgatga cgctcggcac ggtcgtacgc
     3841 agtctcgacc ggctcgacag catcatgccg gcggtcaagg cgttggcggt tcggcatgtc
     3901 gagtttggcg tcaggccgcg gcattacgaa cccgtcggct tcgccctgct gtggacattg
     3961 cagcaagggc tcggtgaggc tttcacgcca gagacgtcgg aggcgtgggc caaagcctac
     4021 ggccttctca gcaaagcgat gattgccgcg gccactgcgc catccgaagt ttgaggcgaa
     4081 cgcaatgtat caggacaaga aatttccgac ctcgccctcg aacgagccgc tgcggctcgt
     4141 catcatcggc aacggcatgg caccaggccg gatgctggaa catctgctcg aagaggcgcc
     4201 ggatcgctat aagatcacga tcttcaacgc cgagccgcgc gtcaactacg accgcatcat
     4261 gctgtcgccc gtcctctcgg gcgagaagga gtacgagcag atcgtcatcc atggcgacgg
     4321 ctggtacatc aagcataata tcacgctcta taaggggcat aagatcgtcg cgatcgaccg
     4381 cgacgcgaag acggtaacct cagaccacgg cgtgacagaa ggctacgaca agctcgtcat
     4441 cgccagcggc tcgacgccaa tcatcctgcc ggtgccgggc aacgatctgg ccggcgttct
     4501 gaccttccgc gatcttgacg atgtgaatgc catgatgctc gccgcgcaat cgcgcgctaa
     4561 ggctgtcgtc atcggcggcg gcctcctcgg cctcgaagcc gcggcggggc tcagttcgca
     4621 cggcatggat gtcaccgtgc tgcacctgat gccgacgctc atggaacgcc agcttgattc
     4681 cgccgccggc tatctgttgc agcaggccgt cgagtcacgc gggatcaagg tgatcaccaa
     4741 ggcgaatacg aaggcgatcc tcggcgacgg caaggtcgag gccgtcgaat tggccgacgg
     4801 cacgatcatt ccagcaacgc ttgtcgtcat ggccgcgggc atccggccca acggcgcttt
     4861 ggccaaacgc gccggtctgg atgtgaaccg cggcattctt gtcgatgccc ataggcagac
     4921 ctccggcccc gacatttcgg caatcggcga atgcgccgaa gtcggcggcc acacctatgg
     4981 ccttgtcgcg ccactctacg atatggcg
//
LOCUS       DASWOG010000305         7065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_245066,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000305 DASWOG010000000
VERSION     DASWOG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245066"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VGB93_06340"
     CDS             <1..1402
                     /locus_tag="VGB93_06340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0145756.1"
                     /translation="AAMDDPGDKPITFDLPSSAPLTLGLDTPASAENQKMIAAFGGEY
                     HDTAIEHFVNDILLKLAKAGEDPEQPYHVTLLNSPVVNAFAMAPDHIYITRGLLALAN
                     DASEVAAVMAHEIGHITARHAALRAEEEKRAALITQAAAVIESRQKGEEVEAVERRTL
                     ASFSRQQELDADEIGIRVMAKAGYDPYAASRFLTTLGRDAELRSSLFGQGSDISKPDL
                     LATHPSTPERVARASQVAEQFGPPGTGITDRTAYLAAIDGMMYGDDPADGSIRGTTFI
                     HPRLGFLFTAPEGFVLENSAQAVLGVKTGGREALRLDSVRLDASMTLESYISSGWIEG
                     LLPSSVETLEVNGMQAVTASARAGEWNFRVAVIRFAGDQVYRMIFAIRSLDADAEKRF
                     RTSIESFRKTTPEEVQDVKPLRIVIVTAQPGDSIESLSNKMAVADHPLEHFELINGLT
                     APGPLTAGQHYKIVAD"
     gene            complement(1472..2239)
                     /locus_tag="VGB93_06345"
     CDS             complement(1472..2239)
                     /locus_tag="VGB93_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0145757.1"
                     /translation="MTQKAPKKAAEAAPAPVRAAAPVAAPKAPTPPVPETKPDVAVDV
                     RPGVPVAEKAKPVKAAPVKHSFKPLEFVVYPAHGVGQILAIEEQEVAGFKLELFVISF
                     SKDKMILKVPTPKSIAVGMRKLASAEVVKRALDTLTGRARIKRTMWSRRAQEYEAKIN
                     SGDLIAIAEVVRDLYRSEAQPEQSYSERQLYEAALDRVAREIASVQKLTDTEALKLID
                     TQLQKSPRRGKAEEGDAEADVEAEASPETEGGIEEAA"
     gene            2828..3217
                     /locus_tag="VGB93_06350"
     CDS             2828..3217
                     /locus_tag="VGB93_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145758.1"
                     /translation="MGFQARARDSGFNLAGFFGLFDGRHTRALLFGLASAARLRDSAN
                     AAKEALPPGDSPRPLDKAQRARARSSNYKSVNLGPQANAAYSEKPPHAPAKHRVNAIR
                     MIKAGPIYEIKISHGTAKIKDLQANNA"
     gene            complement(3288..3626)
                     /gene="fdxA"
                     /locus_tag="VGB93_06355"
     CDS             complement(3288..3626)
                     /gene="fdxA"
                     /locus_tag="VGB93_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY0145759.1"
                     /translation="MPDVVTENCIKCKYTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPADAIKPDTEKGLEKWLKLNADMAKTWPNISQKKAHLPDAKEFDGKQGKFDT
                     YFSPEPGEGD"
     gene            complement(3723..4022)
                     /locus_tag="VGB93_06360"
     CDS             complement(3723..4022)
                     /locus_tag="VGB93_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY0145760.1"
                     /translation="MTLPPVAEKQRLDKWLWFARVVKTRRQAVELIEAGYVRVDAQRV
                     EVPAKLVSPGMVLTVALGRRVRILRITGLAVRRGSADVAEQLFEDLAAQVAPAAI"
     gene            complement(4086..5234)
                     /locus_tag="VGB93_06365"
     CDS             complement(4086..5234)
                     /locus_tag="VGB93_06365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145761.1"
                     /translation="MTGESEALVAEIRDVADPELVAAWRDLYARSLEPNIFLDPDFAR
                     PALSYLQPRGLKMMAVFETGLKRLLALAPVVVPTVPFGLARVYVHKQAALGLPLLDKG
                     AAAAALAKLVDAIRALPGAPSALVFSEIPRDGPTFRLLNELFGEKRRIILSQYERPAL
                     LLASSEPWVRRQTKGDKNSFRRLRRLGERGVLSYRIARGADVSTALTEFLALESSGWK
                     GASKTALASSPDRAGFVQAMAESFARDGKLQIESLDLDGKAVAMGLLIKAAGAGYFWK
                     IAYDEAYAAQSPGILLIRELTGRLSAHRTLRVVDSCAAADHPMIDHIWPERITILDVG
                     ITLTNGPRGRIALAAEGLRRSLRKTAKRLLRRNSSGKAPIAGSWRARS"
     gene            5433..7028
                     /locus_tag="VGB93_06370"
     CDS             5433..7028
                     /locus_tag="VGB93_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0145762.1"
                     /translation="MNYDAIVIGGGHNGLVCAAYLGAAGLKTLVLEAREKIGGCAVTE
                     EFHPGFRNSQAAYTVSLLNPKIIRDLDLQRHGLRIVPRKLANFLPLDDGRYLKLGPGR
                     TKSEVAKFSVKDAERLDAYVGRLDALADFLRAVALETPPNVSAGGLDWLRQLLRAGKF
                     ADRLRKLGLEGERDVLEIFTSSAGDYLDHWFESAPIKAVYGFDAVVGNYQSPYAPGSS
                     YVLLHHCFGEVNGAKGQWGHAIGGMGAISEAMASAARARGVDIRTASPVREVLVEKGR
                     AVGVVTRAGEVFRADKIIANVNPKLLYRDLVPQEALPEDFRDRIMRWRCGSGTFRMNV
                     ALSDLPDFSALPGRELAEHHTAGIIIAPSLAYMEDAYFDARRFGWSKKPIVEMLIPST
                     LDDTLAPRGAHVASLFCQHVAPQLPDGTSWDDHREEVADLMIETVDAIAPNFKASIIA
                     RQIFSPLDLERTFGLVGGDIFHGSLDLGQLFVARPMLGHADYRGPIPGLYMCGSGTHP
                     GGGVTGLPGHNAAREILRDVRRL"
BASE COUNT         1309 a         2146 c         2169 g         1441 t
ORIGIN      
        1 cgcagccatg gatgatccgg gcgacaagcc gatcactttc gatttgccgt cttccgcacc
       61 gctcacgctt ggcctcgaca cgccggcttc cgccgaaaat cagaaaatga tcgcggcctt
      121 tggcggcgaa tatcacgaca cggccatcga gcatttcgtc aatgacatcc tgctgaaatt
      181 ggcgaaggcg ggcgaggatc ccgagcagcc ctatcatgtg acgctgttga attcgccggt
      241 ggtgaatgct ttcgcaatgg cgcccgacca catctatatc acccgcgggc ttctggcttt
      301 ggccaatgat gcgtcggaag tcgctgccgt gatggcgcat gagatcggcc atattaccgc
      361 gcgacatgcc gcgctgcggg cggaggagga aaagcgcgct gcgctgatca ctcaggccgc
      421 ggcagtgatc gaaagccgcc agaaaggcga agaggtcgag gcggtcgaac ggcgtacgtt
      481 ggcgagtttc tcacgccagc aggagcttga cgcggacgag atcggtattc gcgtcatggc
      541 aaaggcgggg tatgaccctt atgcagcatc gcgcttcctg acgacgctcg gccgcgacgc
      601 cgaattgcgc tccagtctct tcggtcaggg ttccgatatc tcgaagcccg atttgctcgc
      661 aacgcatccg tcgacgccgg agcgggtggc gcgcgccagc caggtcgctg agcaattcgg
      721 gccgccggga acggggatca ccgaccgtac cgcctatctc gccgcgatcg atggaatgat
      781 gtacggcgac gatccggccg atggctcgat ccggggcacg accttcattc atccgcggct
      841 tggcttcttg ttcaccgcgc cggagggctt tgtgctggag aactcggcgc aagcggttct
      901 cggggtcaag accggcgggc gcgaggcgct gcggctcgat tctgtgcggc ttgatgcctc
      961 gatgacgctc gaaagctata tttcgtcggg gtggatcgag gggcttctgc cgagctccgt
     1021 cgaaacgctt gaagtcaacg gcatgcaggc cgtcaccgcg agcgcgcgcg cgggcgaatg
     1081 gaatttccgt gtcgcggtga tccgctttgc cggcgatcag gtttatcgga tgatcttcgc
     1141 gatccgcagt ctcgatgccg atgccgagaa gcgctttcgc acgtcgatcg aatcgttccg
     1201 caagacgacg ccggaagaag tccaggatgt gaaaccgctg cgtatcgtca ttgtcaccgc
     1261 acagccgggc gatagtatcg aaagcctgag caataaaatg gcggtcgccg atcatccgct
     1321 cgaacatttc gaactgatca atggccttac cgcgcccggt ccgctgactg ccgggcagca
     1381 ttacaaaatc gtcgcggatt aggcgcggtt tcatcgccaa taaaaaagcc cggcgcagag
     1441 ccgggctttt ggcatcccaa tgaagcgcgt tctatgcggc ttcttcaatt ccgccctcgg
     1501 tctcggggct ggcctcggcc tcgacatcag cctcggcgtc gccctcctcg gctttgccgc
     1561 ggcgcgggct cttttgcaat tgcgtatcga tcagcttaag cgcttccgta tcggtcagtt
     1621 tctgaacgga agcgatttcg cgcgcgacac ggtcgagcgc cgcttcatac aattgccgct
     1681 cggaataaga ctgctcgggc tgcgcttccg aacgatagag gtcgcgcaca acctcggcga
     1741 tggcgatgag atcgccggaa ttgattttcg cctcatattc ctgagcgcga cgcgaccaca
     1801 tcgtccgttt gatgcgggcg cggcccgtca gcgtatcgag cgcccgctta acgacctcgg
     1861 cgctggcgag cttgcgcatg ccgacggcga tcgacttcgg cgtcggcact ttcaggatca
     1921 tcttgtcctt ggaaaagctg atcacgaaaa gctcgagctt gaagccggcg acttcctgct
     1981 cttcgatcgc caggatctgg ccgacgccat gcgccggata gacgacgaat tcgagcggtt
     2041 tgaagctgtg cttcaccggc gcggccttca cgggtttggc tttttcagca accggcacgc
     2101 cgggcctgac gtccaccgca acgtccggct ttgtttccgg caccggaggc gtcggagctt
     2161 tgggcgcagc gacgggcgct gcggcacgaa caggagcagg cgcggcttcc gccgctttct
     2221 ttggggcttt ctgggtcacg ctggctttgc ttgcctcatc atgatgggcg acaggcgggg
     2281 ctgccggcgc tggcttctgc agagaacgca gggccaacac cgcggcaact tgcgcgcggc
     2341 gctcggaatc gaccttctta acggtcggga gttcagtttg aggggcggca gactgccgaa
     2401 ccgcatgaat cgcaactgcc acctttgaaa ctttagtctc aatcggcata gggcgtccag
     2461 actgcgattt gccgcgggtc tcagttgttg accgttttaa ttttggcgcg gtggcttgac
     2521 ttgcagcggt ctttcgcttt gtcgctgcag gcttggcggc cgccgacttc ggggatgccg
     2581 cacgtgcagc cggacgagca gctttcgcag acgcggcctg ctttttcagg cgcgcctgtg
     2641 tcgcgcgaag cttcgcagtc gaagtcttgc tgggtgccgg cgacttcgcc gccgccttct
     2701 tggcgctcga cttcgcagcg gccgatttcg acgcggcccg cgcggcaggc ttggccgccg
     2761 cccggaccga tgcgggacgt gcggcgctgg ccttcgatgc ggccggcttc ttggccttgc
     2821 tcgtttgttg ggatttcagg cccgcgcgcg cgacagtggc tttaaccttg cgggattttt
     2881 cggtcttttt gacggacgac atacgcgcgc actccttttt ggccttgctt cagccgcaag
     2941 gctcagagat tctgcaaatg cagcaaagga agctttgccg cctggggatt cgccgcggcc
     3001 tctcgataag gcacagcgcg cccgggcacg atcatccaac tacaagagcg tgaaccttgg
     3061 cccccaggca aatgctgctt atagtgaaaa gccaccccat gctcccgcca aacatcgggt
     3121 gaacgcgatc cggatgatca aagccgggcc gatttatgaa attaaaatat cacacggaac
     3181 cgcaaaaatc aaagatttgc aggcgaataa cgcttaaaaa cagttgtttt tgccgcaaag
     3241 ctgacgcagg gcggcttgaa ttcgcgattt atgtttgatc tttgaggtta atcgccctcg
     3301 ccaggctcag gcgagaaata ggtatcgaat ttgccctgct tcccgtcgaa ttccttggca
     3361 tcgggcagat gcgctttctt ctgcgagata tttggccaag tcttggccat atccgcatta
     3421 agcttcaacc atttttccag acccttctcg gtatcgggct tgatggcgtc ggcggggcat
     3481 tcgggttcgc atacgccgca atcgatgcat tcgtcggggt gaatgacgag catattctcc
     3541 ccttcgtaga agcaatcgac cgggcagacc tccacgcagt ccgtatactt gcacttgatg
     3601 cagttttcgg tgacgacgtc gggcatgaat ctcctccgcg cgggggcggt tccacgatga
     3661 gttctgcggt ttgctagcgt ctttgcggcg cttaagcaag caattgccgc acggcaccaa
     3721 gcttatattg ccgcaggcgc gacctgcgcc gccaggtcct cgaaaagctg ttctgcgaca
     3781 tcggcactcc cgcgccgcac cgccaatcct gtgattcgca ggatgcgaac gcggcgaccg
     3841 agtgcgacgg tcaaaaccat gcctggcgag acaagctttg ccggaacttc gaccctttgc
     3901 gcgtcaaccc gaacatagcc agcttcgata agctcgaccg cctgtctgcg ggttttcacg
     3961 acgcgcgcga accagagcca tttgtcgagc cgctgttttt cggcgacggg gggcaaggtc
     4021 attttcgcgg ccatcaagac tcccgcaagg gatgcacgat gacctatgca tgtcgccacg
     4081 gccgatcaag accgagcgcg ccagcttccc gcgatcggcg ccttgccgct ggagttccgc
     4141 cgcagcaggc gtttggccgt cttgcgcagg gatcgacgca gaccttcggc ggcgagagca
     4201 atccggccgc gcgggccgtt cgtcagcgtt attccgacgt cgaggatcgt aatgcgctcc
     4261 ggccagatat gatcgatcat tggatggtcc gccgctgcgc aggaatcgac cacacgcaag
     4321 gtccgatgcg ccgacaggcg gccagtcagt tcgcgaatca ggaggatgcc cggcgattgc
     4381 gcggcgtagg cctcatcata ggcaatcttc cagaaataac cggcgccggc ggccttgatc
     4441 agaaggccca tcgcgacggc cttgccgtcc agatcgaggc tttcgatctg cagcttgccg
     4501 tccctcgcga aactttccgc catggcttgg acgaaaccgg cgcgatccgg cgacgaggcg
     4561 agcgcggttt tgctggcgcc tttccacccg gatgattcga gggcaaggaa ttccgtcaac
     4621 gccgtggaaa cgtccgcgcc gcgggcaatt cggtaggaca gcacaccgcg ctcaccaaga
     4681 cggcgcaacc ggcggaaaga gtttttatcg cctttcgtct ggcggcggac ccagggctcc
     4741 gacgacgcga gcaacagcgc cgggcgttcg tattggctca ggataatgcg ccgcttctcg
     4801 ccgaagagct cgtttaggag cctgaaggtc gggccgtcgc gcggaatttc actgaagaca
     4861 agggcgcttg gtgcgccggg cagcgcgcga atggcatcca cgagctttgc cagcgcagcg
     4921 gctgccgcgc ctttgtcgag caacggcagg ccgagtgccg cttgcttatg cacatagacg
     4981 cgtgcaaggc cgaacggcac ggtcggcaca acgactggcg ccaacgccaa aagccgtttc
     5041 agtcccgtct cgaacaccgc catcattttg aggccgcgcg gctgcagata ggagagtgcc
     5101 ggccgggcga agtcgggatc caggaaaata ttcggctcga gactgcgggc gtaaagatcg
     5161 cgccacgccg cgacaagttc cggatccgcg acatcgcgaa tctcggcgac gagagcctcc
     5221 gattcgccgg tcaccggggt gtgttctcct tggcgcgact cgaagaccga tgcgctcgga
     5281 aagctttggc cgacatcgag agtcgcggaa gcttgcatca cgcgacagga cacgcttgaa
     5341 cgcgaaaaag acctatctat ccctatcata atcggacgcg gccggttttt caggttgaat
     5401 aatcttttaa ccctaacaga ggtgccgtcg cggtgaatta tgatgcgatc gtgatcggcg
     5461 gcggccataa cgggcttgtc tgcgcggcct atctcggcgc agccgggcta aagaccctgg
     5521 tgctcgaagc gcgcgagaaa attggcggct gcgcggtcac cgaggaattt catccgggct
     5581 tccgcaattc gcaggcagcc tacacagtct cgctgctgaa tccaaagatc atccgcgatc
     5641 tagatctgca gcgtcacggg ctgcggatcg tgccgcgcaa gctcgcgaat tttctaccgc
     5701 tcgatgacgg ccgctatttg aagcttggtc ccgggcgcac gaaaagcgag gtcgcaaaat
     5761 tctcggtgaa agacgcggaa cggctcgatg cctatgtcgg gcggctcgat gcgctcgccg
     5821 attttctccg cgccgtcgcg ctggaaacgc cgcccaatgt ttcggcgggt ggactcgact
     5881 ggctgcggca acttctccgc gccggaaaat ttgccgatcg tttgcgaaag ctcggtctcg
     5941 aaggcgagcg cgacgtgctc gaaatcttca cgtcgtccgc cggagattat ctcgatcatt
     6001 ggttcgagag cgcgccgatc aaggcggtct atggcttcga tgccgtggtc ggcaattatc
     6061 aaagtcccta cgcgccgggt tcaagctatg tgttgctgca tcattgcttc ggcgaggtaa
     6121 acggtgcgaa gggccaatgg ggccatgcca tcggcggtat gggcgcaatc agcgaagcga
     6181 tggcgagcgc ggcgcgtgcg cgcggcgttg atattcgtac cgcaagcccg gtgcgcgaag
     6241 tgctggtcga gaaaggtcgc gccgtcggcg tcgtgacgcg cgcgggcgag gttttccgcg
     6301 ccgataagat catcgccaac gtcaatccga aattgctgta tcgcgatctc gtcccgcagg
     6361 aggctttgcc ggaggatttc cgcgacagaa tcatgcgctg gcgctgcggc tcgggcacat
     6421 tccgcatgaa tgtcgcgctg tcggacctgc cggatttttc cgctttaccg ggccgcgaac
     6481 ttgccgagca tcacaccgct ggcatcatca tcgcgccgtc gctcgcctat atggaagacg
     6541 cttatttcga tgcgcggcgc ttcggatggt cgaaaaagcc catcgtcgaa atgctcattc
     6601 cctcgacact cgacgatacg ctggcgccgc gcggcgcgca tgtcgcgagc ctcttctgcc
     6661 agcatgtcgc gccacaattg cccgacggca catcatggga cgatcatcgc gaagaggtcg
     6721 ccgacctgat gattgaaacg gtcgacgcca tcgcgccgaa tttcaaggca tcaatcatcg
     6781 cgcggcaaat cttctcgccg ctcgatctcg aacgcacctt cggcctcgtc ggcggcgata
     6841 tttttcacgg gagcctcgat ctcggccaat tgtttgttgc acggccgatg ctcgggcacg
     6901 cggattatcg cggcccaatt ccgggcctct acatgtgcgg gtccggcacg catcccggcg
     6961 gcggcgtcac cggcctgccc ggccataacg cagcacgcga gattttacgg gatgtcaggc
     7021 gcctctaacc ctgccattgt ccaactcgac cttcgccttt aggct
//
LOCUS       DASWOG010000306         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_98787,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000306 DASWOG010000000
VERSION     DASWOG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98787"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VGB93_06375"
     CDS             <1..1058
                     /locus_tag="VGB93_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590925.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY0145763.1"
                     /translation="FDLFDSSRKGRGKLYVRHVLIAADADLLPSWLRFVRIVVDSADL
                     PLTMSRELIQQSTVFAAIKKVVAYRVMHDMTKLEENDPEAYAKVWTNFGAVLKEGLYE
                     DPERRDALFKLARFASTIDLDGKRTLAAYVGALRPNQTAIYYRNGDDLKALAASPQLE
                     GFRARGIEVLLLSDPVDAFWVSSAVGFDGKPFKSVSQGAADIKAVPLLEGNEEKAAEP
                     PSAALASLFALMKQELEGVAADVRASDRLSESPACLIAPEHGFDRRLERILAEHGQLK
                     DVAKPILEINPTHPLIAALAAQVGHADQAALADIVWLVFDEARLLDGEKPANAADFAA
                     RLTRVLTKAVATPATKE"
     gene            1055..1942
                     /gene="prmC"
                     /locus_tag="VGB93_06380"
     CDS             1055..1942
                     /gene="prmC"
                     /locus_tag="VGB93_06380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0145764.1"
                     /translation="MIASGVGFDPGLSRAEAQRLLTQAFAAAKIETPALDARLLLCAA
                     LGIGHLDLLSEPDRQISAAAGEIEATARRRLAGEPVARIIGRREFYGLDFALTPAVLD
                     PRPDTEILVDAVLVALEARRAEPLHLLDLGLGSGAILAALLSQLPAAFGVGVDRSEPA
                     CRVARSNLAALGFAGRSAIVCADWTNPLRGAFDAIASNPPYIATGEIAGLAAEVRAHD
                     PRGALDGGADGLAAYRILAATVPQLLRPGGIVAFEVGATQAESVAGLLGATGMLESRD
                     FLRDLGGHVRVVTAVRLVC"
     gene            2353..>2765
                     /locus_tag="VGB93_06385"
     CDS             2353..>2765
                     /locus_tag="VGB93_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY0145765.1"
                     /translation="MRPNQNNKRMRGRNNNNNNSSNRKGPNPLSRSYESNGPDVKIRG
                     TAHHIGEKYLQLARDAQSSGDPVMAESYLQHAEHYFRIIATAQQAQQAQFAGYNRPGT
                     EADNEELEDEDDFSGLPDRFASPVAPNVTPPPQPP"
BASE COUNT          515 a          830 c          877 g          543 t
ORIGIN      
        1 cgttcgatct cttcgatagt tcgcgcaagg ggcgcggcaa gctttatgtc cgccatgtgc
       61 tcatcgcggc ggacgccgat ctgctgccaa gctggctgcg tttcgtacgg attgtcgtcg
      121 attccgcgga tctgccgctc accatgtcgc gtgagctgat ccagcagagc accgtttttg
      181 ccgcgatcaa gaaagttgta gcctatcgcg tcatgcacga catgaccaag ctcgaagaga
      241 acgatcccga agcttacgca aaagtctgga cgaacttcgg tgcggtgctg aaagagggtc
      301 tttacgaaga cccggaacgg cgcgacgctc tcttcaagct cgcgcgtttc gcctcgacca
      361 ttgatctcga tggcaagcgc acgctcgccg cctatgtcgg cgcgctacgc ccaaatcaaa
      421 cggctatcta ttatcgcaat ggcgacgact tgaaggccct ggcggcgagt ccgcagctcg
      481 aaggtttccg cgcccgcggt attgaggttt tgctgctcag cgatccggtc gatgcttttt
      541 gggtgtcttc ggcggtgggc tttgacggca aaccgttcaa gtcggtttcg caaggggcgg
      601 ccgacatcaa ggcggttcct ttgctggagg gcaacgaaga aaaggcggct gaaccgccgt
      661 cggcggcgct ggcgtcgctc ttcgctctga tgaagcagga gttggaaggc gtggccgcag
      721 acgtgcgcgc gtcggaccgg ctgtccgaaa gcccggcctg cctgatcgcg ccggaacatg
      781 gtttcgaccg gcggcttgaa cgcatcctcg ccgagcacgg ccagttgaag gatgtcgcca
      841 agccaatcct cgagatcaat ccgacgcatc cgctcatcgc ggcgcttgcg gcgcaagttg
      901 gtcacgccga ccaggccgcg cttgccgata ttgtctggct tgtttttgac gaggcccgtc
      961 tgctcgacgg cgagaaaccg gcaaacgccg cggattttgc cgccagactc acgcgcgtgt
     1021 tgaccaaggc cgtggcaacg cctgcaacga aggaatgatc gcgtcaggcg ttggcttcga
     1081 tcccggtctt tcgcgtgccg aagcccagcg cctgctgacg caggctttcg cggcggcgaa
     1141 gatcgaaacg ccggcgctcg atgcccgcct gcttctctgc gcggcgctcg gtatcggcca
     1201 tctcgatctg ctaagtgagc cggatcggca gattagcgcc gcggcgggcg agatcgaagc
     1261 cacggcgcgg cgccggcttg ccggcgagcc ggtcgcgcgg attatcggac gtcgcgagtt
     1321 ttacgggctc gatttcgcgc taacgccggc ggtcctcgac ccgcgaccgg acacagaaat
     1381 tctcgtcgac gcagtattgg ttgccttgga agcacgacgc gccgagccgc tgcatctgct
     1441 cgatctcgga ttgggttccg gcgcaattct cgcggcactg ctgtcgcaat taccggcggc
     1501 tttcggtgtc ggcgttgatc gttcggagcc agcgtgccgc gtggcgcgca gcaatcttgc
     1561 ggcgctcggg tttgccgggc gcagcgctat cgtttgcgcc gattggacga atccgttacg
     1621 cggcgccttc gacgccatcg cctcgaatcc gccctatatc gcgacggggg agatcgctgg
     1681 cctcgcggcg gaggtgcgcg cgcacgaccc gcgtggcgcg cttgacggcg gcgctgacgg
     1741 gctcgcggcc taccgcattt tggcagcgac cgtgccgcaa cttctcaggc cgggcgggat
     1801 tgtcgctttt gaagtcgggg cgacccaagc cgaatcggtg gcgggcctac tgggggctac
     1861 gggtatgctg gagtcgcgag attttttgcg cgaccttggc ggccatgtcc gagttgtgac
     1921 cgccgttcgt ctcgtatgct gactgatcga aatacgttgc atatcggcaa catacgcggg
     1981 aggcgcggaa aatttttaca aaagtgcttg gcgcagccgg ggaaaacgat tacgttccgg
     2041 cgcaaagatc gcttttcgcg aatgttaatt gaggcagcgt cgagatcccc acaccggacg
     2101 tccggatgga atgatttccg ccactcaaga gcctttagtg ctcgcgcgat tgaggcgatt
     2161 gtccccaacg tctgagatcc cgagagctgc ggggtcagcc tcatgcggtg ccgaaccgga
     2221 ggcttagcgt tagcagctgg agaacggaag ccagcctatc ctttgtgcct tagggccagt
     2281 ggtgtcgtgc cgactcgggg tgttccgggg cgtcggagtg gatgggttat aagaaacatc
     2341 caaggatgat cgatgagacc aaatcagaat aacaagcgca tgcgcggccg gaacaataac
     2401 aacaacaata gcagcaatcg taagggccca aatccattat cgcgttccta tgagtcgaat
     2461 ggtcccgacg ttaagatcag gggcaccgcg caccacatcg gcgaaaaata tctgcagctc
     2521 gcccgcgacg cacaatcgtc cggcgacccc gtgatggctg aaagctatct gcagcacgcc
     2581 gaacattatt tccgcatcat cgccaccgct caacaggcgc agcaggcaca attcgcgggc
     2641 tataaccgtc cgggaaccga ggcggacaac gaggagcttg aagacgaaga cgacttcagc
     2701 gggcttccgg atcgtttcgc gtcgccggtg gctcccaatg tcacgccgcc tccgcagccg
     2761 ccgca
//
LOCUS       DASWOG010000307         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_190521,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000307 DASWOG010000000
VERSION     DASWOG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190521"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..911
                     /locus_tag="VGB93_06390"
     CDS             54..911
                     /locus_tag="VGB93_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0145766.1"
                     /translation="MGAQVVGLRRRRVAYSPDLRVLVLANQKGGVGKTTTAINLGTAL
                     AAIGERVLIIDLDPQGNASTGLGIDRKSRGVSTFDVMLGEEPLKAAIQPTAVPRLSIA
                     PSTLDLLGVELEIASDKDRAYRLKNALKVFADGQSGQPDDNRFSYVLIDCPPSLNLLT
                     VNALAASDSVVVPLQCEFFALEGLSQLLSTVESVKQTLNADLTIHGIVLTMYDPRNNL
                     ATQVAADVRGFMGSKVYETIIPRNVRISEAPSYGKPVLLYDLKCAGSQAYLRLASEVI
                     QRERQLRAA"
     gene            949..1812
                     /locus_tag="VGB93_06395"
     CDS             949..1812
                     /locus_tag="VGB93_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0145767.1"
                     /translation="MADEHRPRLGRGLAALIGDAGEENAAIARSRGQKKIPLAFLRPN
                     PRNPRKDFNDQDLDDLVASIREKGIIQPILARTIAGHADAYEIIAGERRWRAAQRVGL
                     HDVPVILVEADDKQALELAIIENVQRADLNALEEALGYQQLEAEYNYTQNDLAKVIGK
                     SRSHVANTLRLLKLPEKTKRLLAEGQLSAGHARALLTRADADEIASRIVAQGLSVRDV
                     EKLTQESSPRAKTKNDVIEKDADTRALEKELSDVLGLLVAINHHGEAGEVRIRYKSLE
                     QLDDLIRKLKR"
     gene            complement(1882..2316)
                     /locus_tag="VGB93_06400"
     CDS             complement(1882..2316)
                     /locus_tag="VGB93_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0145768.1"
                     /translation="MPNSKYAGYENVKPYFELVRGALGDLVDGEHFADVVSNDVAYEV
                     LYNFPGWPRIIRGRADLMAAFAGYGDNIKVQSADKLTTHRTDDGRVVVIEYEVHGTIL
                     ATGVKYNNRFCSIIKIESRKIAHWRDYMDSLAAWNALTAAAR"
     gene            complement(2386..3402)
                     /gene="holA"
                     /locus_tag="VGB93_06405"
     CDS             complement(2386..3402)
                     /gene="holA"
                     /locus_tag="VGB93_06405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0145769.1"
                     /translation="MVAVKNGEADRFIAKPAPNIFLYLVFGTDAGLIAERVQKIIAAS
                     VDDLKDPFQFLRISGDELAADPLRLADEANTIPMFGGRRAIAIDAQGKSFVAALEPIL
                     AKPPLDCMIVIEAGALKRDAPLRKLCESNPRAAALECYPDSAKDVAQLIDSELRADNL
                     SIDADARTLLVSRLGEDRLTTRAEIAKLALYARGAGTVTVDHIAAIVAEASSLALDTA
                     IDGAFEGDLRAVEKTAGRVFSEGGDYNALLGAALRHATALHRARLDAESGRGPGGGGG
                     FGFRRSPAFERHVRAWPSERLSRTIIALNQAIGKARRDPRLADQIALRTLWSIALSAR
                     KKSG"
     gene            complement(3402..>3698)
                     /locus_tag="VGB93_06410"
     CDS             complement(3402..>3698)
                     /locus_tag="VGB93_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145770.1"
                     /translation="DPQYHLVVTLTEGTETPLIDTVEGIATSATIVTTANFRLIPTAN
                     KKVYVGKAYVAASYDRNINRFANVRAARDAEIRDARRLADEITTQVAAELATHP"
BASE COUNT          764 a         1064 c         1134 g          736 t
ORIGIN      
        1 ttggcggcgt gacgcgcgaa aagtctccaa aaagaatcat ttagagatat cgcatgggtg
       61 cgcaggtagt tggattacgg agacggcgtg tggcttattc tcccgatctt cgcgttctag
      121 tgctcgccaa tcaaaagggc ggcgtcggca agacgacgac ggcgatcaat cttggtacag
      181 cgctcgccgc catcggcgag cgagtgttga tcatcgacct cgatccgcag ggcaatgcct
      241 cgacggggct tggcattgac cgcaagagcc gcggcgtctc gaccttcgat gtcatgcttg
      301 gcgaagagcc gttgaaagcg gctatccagc caacggccgt gccgcggctt tcgattgcgc
      361 cgtccaccct tgatcttctc ggcgtcgaac tcgaaattgc ctcggacaaa gatcgcgcct
      421 accggctgaa aaatgccctg aaagtctttg cggatgggca gtcgggccag ccggacgaca
      481 atcgtttctc gtatgttctc atcgactgtc cgccttcgct taatctgctg accgtcaacg
      541 ctctcgcggc gagcgatagc gtcgtggtgc cgctacaatg cgaattcttc gccctcgaag
      601 gtctttcgca attgctttca acagtcgaga gcgtcaagca gacgctgaac gccgatctta
      661 cgattcatgg gatcgtgctg accatgtacg acccgcgtaa taatctcgcg acccaggttg
      721 ccgccgatgt tcgcggcttc atgggcagca aggtttacga gacgatcatc ccgcgcaacg
      781 tgcggatttc cgaggcgccg tcgtatggga agccggtgct tctttacgat ctcaaatgcg
      841 ctggcagcca ggcctatctg cggctggctt cggaagtgat ccagcgcgaa cggcaattgc
      901 gcgcggcgtg ataaccgaat caagaaggct catatgaagg cggtgaccat ggcagacgaa
      961 caccggccgc gactgggacg cgggctggcg gcattgatcg gcgatgccgg cgaagaaaat
     1021 gcggcgatcg ctcgctcgcg tggccaaaag aaaattccgc tcgcgtttct gcggcccaat
     1081 ccgcgcaatc cgcgcaagga tttcaacgat caggatcttg atgatctcgt cgcgtcgatc
     1141 cgcgaaaagg gaatcattca gccgatcctc gctcgcacga tcgcggggca tgccgatgcg
     1201 tatgagatta tcgccggcga acggcgctgg cgcgcggcgc agcgtgtcgg gctgcatgat
     1261 gtgccggtca tactcgtcga agcggacgac aagcaggcac tcgaactcgc gatcatcgaa
     1321 aacgtgcagc gcgccgatct caacgcgctg gaagaagcgc tcggctatca gcagctcgaa
     1381 gcagaatata attatacgca gaacgatctt gcgaaagtca tcggcaaaag ccgcagccat
     1441 gtcgccaata cgttgcggct gttgaaactg ccggaaaaga caaaacgtct gctggccgaa
     1501 ggccagcttt cggcaggaca tgcacgcgcc ttgttgacgc gggccgatgc ggacgagatc
     1561 gcgtcgcgca ttgtggcgca ggggctttcg gtacgtgacg ttgaaaagct cacgcaggaa
     1621 tcttcgccgc gcgccaaaac gaaaaacgac gtgatcgaaa aagacgccga cacgcgtgcg
     1681 ctggaaaaag aattaagtga cgtgctcgga cttttggttg ccattaacca tcacggcgaa
     1741 gcgggcgaag tccgtattcg ctacaagagc cttgaacaac tcgacgacct cattcggaaa
     1801 ttgaagcgct aaaactttgc ccgtcattgc gagcgaagcg aagacctatc gcgtagcagc
     1861 atggccaatc gtctcggatt tttagcgagc ggccgctgtc agcgcgttcc atgccgcaag
     1921 ggagtccata tagtctctcc agtgcgcaat tttccgactt tcaatcttga tgattgagca
     1981 gaaccgattg ttgtacttca cgcccgtcgc gagaatggtc ccatggactt catactcgat
     2041 gacgacgacg cggccgtcat ccgtcctgtg ggtggtcagc ttgtccgcag actggacctt
     2101 gatgttgtcg ccgtagccag cgaacgcagc catcagatcg gcccggcccc ggataatgcg
     2161 aggccagccg ggaaagttgt agaggacctc gtaggcgacg tcattcgaga caacatcggc
     2221 aaagtgctcg ccatcgacca agtcgccgag cgcccctcgg accagctcga agtaaggctt
     2281 gacgttctca tagccagcgt atttggagtt tggcattccg gactttccga catttcgcga
     2341 cgttgcttgt gaaaagaatt gcataagtga ggccggctaa agaaattagc ccgacttctt
     2401 cctagcactg agcgcgatcg accacagcgt acgcaatgcg atctgatcgg cgagacgcgg
     2461 atcgcgccgg gctttgccga tggcctgatt gagcgcgatg atggtgcgcg acaatcgctc
     2521 cgacggccac gcgcgaacat gccgttcgaa ggcggggctg cggcgaaaac caaacccgcc
     2581 accaccaccc ggtccgcgcc cggattccgc gtcgagccgg gcgcgatgca gcgctgtcgc
     2641 atggcgtaac gcggcgccga gtaaggcatt gtaatccccg ccttcggaaa agacgcgtcc
     2701 ggcggttttt tcgaccgcgc gaagatcacc ttcaaacgca ccgtcgatgg cggtgtctag
     2761 cgcgaggctt gacgcctcgg cgacgatcgc cgcgatatga tcgaccgtta ctgttccggc
     2821 tccccgcgcg tagagcgcga gtttggcgat ctccgcgcgc gtcgtcagcc gatcttcgcc
     2881 cagccgcgaa acgagcagcg tccgtgcgtc ggcgtcgatg ctgagattgt cggcacgcaa
     2941 ttcactgtcg atgagctgcg cgacatcctt ggcggaatcc ggatagcatt cgagcgccgc
     3001 agcgcgcgga tttgactcgc aaagtttgcg taagggggcg tcgcgcttga gtgcgcccgc
     3061 ttcgatcacg atcatgcaat cgaggggcgg ctttgcgagg atgggttcga gggccgcgac
     3121 aaaactctta ccttgcgcgt cgatcgcgat ggcgcgccgg ccgccgaaca tcgggatcgt
     3181 gttggcttcg tcggcgaggc gcagcggatc ggcggccaac tcgtcgccgg aaatccgcag
     3241 aaattggaac gggtctttca aatcgtcgac gctggcggcg atgatttttt gtacgcgctc
     3301 ggcaatcagg ccggcgtcgg tgccgaagac gagataaagg aaaatattcg gtgccggctt
     3361 ggcgatgaaa cggtcggctt caccgttctt gaccgcgacc atcagggatg ggtggcgagt
     3421 tcggcggcga cctgcgtggt gatctcatcc gccaggcggc gcgcgtcccg aatttccgcg
     3481 tcacgcgcgg cgcgcacatt ggcaaagcga ttgatgttgc gatcgtagct cgccgcgaca
     3541 taggccttgc cgacgtaaac ttttttgttg gcggtcggga tgaggcggaa atttgctgtc
     3601 gtcacgatgg tcgccgacgt cgcaatgcct tccaccgtgt cgatcagcgg cgtctcggtg
     3661 ccctcagtga gcgtcacgac gaggtggtac tgcggatc
//
LOCUS       DASWOG010000308         5697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_116925,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000308 DASWOG010000000
VERSION     DASWOG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116925"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1451
                     /locus_tag="VGB93_06415"
     CDS             66..1451
                     /locus_tag="VGB93_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEY0145771.1"
                     /translation="MPRRRSTHLLRGGFALFAAAADFAAIAICAYLAHLALNGAPFSL
                     PPGTYARLGLLMSTFFISVAAIRGDYGVMKYLTFDRLLQRAIIPWGVAILCTLMLLVS
                     RRPLSNANALALFILFAGGFIAVNAVRLLITIHTRARARQGGVAAHRIFLLGHEKEIE
                     AFTKRFEPWIYGVHIVAAAVLRGSDSLEEDLTLARASARMLKPDDVFILIPWDDKASI
                     DAAVQAFLHVPTSIHLGPERVLDRFTDARVTKIGPVSSLNIVRDPLNLPERIAKRTFD
                     VVLSALGLLMLSPLFCMIALAIKLDSRGPVIFRQRRYGFNQQPFRIYKFRSMSTLEDS
                     ADLTLVSKGDARVTRVGDFIRRHNIDELPQLFNVLLGDMSLVGPRPHALVIDQMFERR
                     IALYARRHNMKPGITGWAQINGYRGGMSDDRMRARVEHDLFYVDNWSMWLDIEILWLT
                     LTSKRGYTNAF"
     gene            complement(1433..3049)
                     /locus_tag="VGB93_06420"
     CDS             complement(1433..3049)
                     /locus_tag="VGB93_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY0145772.1"
                     /translation="MTQSTAPDPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIALAG
                     EVRAKANRRQTRSDWMGIERERGISVVTSVMTFEYGGRVFNLLDTPGHEDFSEDTYRT
                     LTAVDSAIMVIDAAKGIEARTRKLFEVCRLRDIPIVTFINKLDRESRDPFDLLDEIEK
                     TLALDAAPITWPLGSGRDFAGVYDLRKNVVRRLDAADQPLSVTGHDDPALMAILPPLE
                     AEAIRDQLELARGALKSFDKQAFLEGHLTPVLFGSALRTFGVADLIDALAEFAPPPRG
                     LEAASRHVDPREPKMSGFVFKIQANMDPNHRDRIAFMRVCSGKLVRGMKAKLVRTGKN
                     LALNAPQFFFAQDRQLADEAYPGDVVGIPNHGTLRIGDTLTEGEDLVFRGVPSFAPEI
                     LRRVKISDAMRAKKLREALQQMAEEGVVQLFLPNDGSNAMVGVVGALQLDVLVERLAA
                     EYGLQVSLETSRFELARWVSSEAPAELEKFARAYPSSMAVDLDGSPVYLAASAWDLRY
                     EQEKWPQVIFADIKDYQKAETGKGRQKALV"
     gene            complement(3075..4040)
                     /locus_tag="VGB93_06425"
     CDS             complement(3075..4040)
                     /locus_tag="VGB93_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0145773.1"
                     /translation="MIYVQSEERHKLGRFADDATAGMRKDVPLVPATSIAGRALVTVV
                     AIMTFLAGLTAGAAMLIAHSADDWRGQVAREMTIQVRPVDGRDLDADTTAAVAAARTA
                     SGIADVYAYSKAQSEALLAPWLGTGLDLSELPVPRLIVVKLDPKGTLDVEALRKAVMD
                     KVPNATVDDHRLWLQRLSVMAGTVVVIAFVIFALVMLAMVLAVGFATRGAMAGNREII
                     EVLHLVGAADGYISRQFQRHFFRLGLRGGLIGGGGALLVFLLSSSISALMRATAGGDQ
                     IEAMFGSFSLSLKGYALIALIAGAVAVTTGFVSRIIVFRHLRRLT"
     gene            complement(4047..4709)
                     /gene="ftsE"
                     /locus_tag="VGB93_06430"
     CDS             complement(4047..4709)
                     /gene="ftsE"
                     /locus_tag="VGB93_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY0145774.1"
                     /translation="MVRFENVGLRYGMGNEILKDISFEIEPHSFQFLTGPSGAGKTTL
                     MRLILLSLKPTRGLISLFNRDSAQLSKDQITDLRRRVGVVFQDFRLLDHLTTYENVAL
                     PLRVQGRDESAYRAEVVELLRWVGLGDHMHVLPPVLSGGEKQRAAIARALIVRPELLL
                     ADEPTGNVDPTLARRLLRLFAELHKSGTAVVIATHDLALMDQFETARRLVLGDGRLHI
                     YE"
     gene            4843..5535
                     /locus_tag="VGB93_06435"
     CDS             4843..5535
                     /locus_tag="VGB93_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY0145775.1"
                     /translation="MLIDCPGCAASYHITKAALSPSGRRVACPRCETVWLATPCLPEV
                     AGFIKAAPHFSPEEEPPPRQTDYVRAVAAPLPRALRPRGGGFAGKFLVGTAALALAMG
                     LIASRDAIARVWPAADRLYADIGIPVGQRSLAIRDLHTVLTRTNGEVFLGVEGIIFNQ
                     RQEETAVPPIRLAILDAAGHELYTWQVAPARHVLAGGDSLPFRARLAEPPQDGRDVVA
                     HFATPDEIVADR"
BASE COUNT         1060 a         1852 c         1735 g         1050 t
ORIGIN      
        1 ccgcctgaat gtttcgcggc aaaacagagc cagggccgcg cgcctatgcg agttgggcgc
       61 aagcgttgcc acgccgccgc agcacgcatc tgctgcgggg cggcttcgcc ttgttcgccg
      121 ctgcggctga tttcgccgcg atcgcaatct gcgcctatct cgcacatctc gcgctcaacg
      181 gcgccccctt cagcctgccg cccgggacct atgcccgcct cggccttctg atgtcgacct
      241 ttttcatctc cgtcgccgcg atccgcggcg attacggcgt gatgaaatat ttgaccttcg
      301 accgtctgct gcagcgcgcg atcatccctt ggggagtcgc cattctctgc acgctcatgt
      361 tgctggtttc acggcgtccg ctttcgaacg ctaacgcgct cgcgcttttc atcctgttcg
      421 ccggcggctt catcgcggtc aatgccgtcc ggctcctcat cacgatccac acccgcgccc
      481 gcgcacgcca aggcggggtt gccgcacatc gcatctttct tctcggccat gagaaggaaa
      541 tcgaagcctt cacgaagcgt ttcgagccct ggatctacgg cgttcatatc gtcgcggccg
      601 ctgttctgcg gggttcagac agtcttgaag aagacctcac cctggcccgc gcctcggcgc
      661 ggatgctgaa gcccgacgat gttttcatcc tcatcccatg ggacgacaag gcgtcgatcg
      721 acgccgccgt gcaggccttt ctgcatgtgc cgacgtcgat tcatctcggt cccgagcgcg
      781 tgctcgatcg ttttaccgat gcacgcgtca ccaagatcgg gccggtatca agcctcaata
      841 tcgtccgcga cccgctgaat ttgccggaac gaatcgccaa gcgcacgttt gacgtcgtcc
      901 tctccgcact tggtctgttg atgctctcgc cgttgttttg catgatcgca cttgcgatca
      961 aactcgacag ccgcggcccc gtgatcttcc ggcagcggcg ttacggcttc aatcaacagc
     1021 cctttcgtat ctataaattt cgctcgatga gcacgctgga ggacagcgct gaccttacgc
     1081 tcgtctcgaa gggcgacgca cgcgtgacgc gtgtcggcga tttcatccgc cggcacaata
     1141 tcgacgaact accgcaactc tttaacgtcc tcctcggcga tatgtcgctc gtcggcccgc
     1201 gcccgcacgc gctcgtcatc gatcagatgt tcgagcggcg tatcgctctc tacgcacgcc
     1261 ggcacaatat gaagcctggc atcaccggtt gggcacagat caacggctat cgcggtggca
     1321 tgagcgacga ccggatgcgc gcccgcgtgg agcacgatct cttttatgtc gataattggt
     1381 cgatgtggct cgacatcgaa attctctggc tgacgctgac gagcaagcgc ggctacacca
     1441 acgccttctg acggcccttg ccggtttccg ctttctggta gtccttgata tcggcaaaaa
     1501 taacctgcgg ccatttctcc tgctcgtagc gcaggtccca cgcggaggcg gcgagataga
     1561 ccggcgagcc gtcgagatcg accgccatcg acgacggata ggcccgggcg aatttctcaa
     1621 gctccgccgg ggcttctgaa ctcacccagc gcgcaagctc gaagcgcgac gtctccaggg
     1681 acacctgaag cccgtattcg gccgcgagcc gttcgacgag aacgtctagc tgcaaagcgc
     1741 cgacgacgcc aaccatagca ttcgagccgt cattcggcag gaagagttgc acgacgccct
     1801 cctccgccat ctgctgcaag gcttcgcgta atttcttggc gcgcatcgca tcggagattt
     1861 tgacccggcg caggatttcc ggcgcgaagc tcggcacacc acggaacacg aggtcttcgc
     1921 cttcggtcag cgtatcgccg atgcgcaacg tgccgtggtt cggaatgccg acgacgtcac
     1981 cgggataggc ttcatcggca agctgacgat cctgcgcgaa gaagaattgc ggcgcgttga
     2041 gcgccaagtt tttgccggtg cggacgagct tcgctttcat cccccgcacg agcttgccag
     2101 agcagacgcg catgaaggcg atccggtcgc gatggttcgg atccatgttc gcctgaatct
     2161 tgaagacgaa cccgctcatt ttcggctcgc gcggatcgac atggcggctc gccgcttcca
     2221 gcccgcgtgg cggcggtgcg aattccgcga gcgcatcgat gagatcggcg acgccaaacg
     2281 tgcgcagcgc actgccgaat aaaaccggcg tcaaatggcc ttcgaggaaa gcctgcttat
     2341 cgaatgactt gagcgcgccg cgcgcgagtt cgagctggtc gcggatcgcc tcggcttcga
     2401 gcggtggaag aattgccatc agtgccggat cgtcatgtcc cgtgacgctc aacggttgat
     2461 ccgccgcatc cagccggcgc acgacgttct tgcgaagatc gtagacgccg gcgaaatcgc
     2521 ggcccgagcc cagcggccag gtgatcggcg cggcatcgag cgccagcgtc ttctcgatct
     2581 cgtcgagaag atcgaacgga tcgcggcttt cgcggtcgag tttgttgatg aaggtgacga
     2641 tcggaatgtc gcgcagccgg cagacttcga agagtttgcg cgtgcgggcc tcaatgccct
     2701 tggcggcgtc gatcaccatg atcgccgaat cgacggcggt gagcgtgcga taggtgtcct
     2761 cggaaaagtc ctcgtggccc ggcgtgtcga gcaggttgaa gacgcggccg ccatattcaa
     2821 acgtcatcac cgacgtgacg acggagatgc cgcgctcgcg ctcgatgccc atccagtcgg
     2881 accgcgtctg gcggcggttg gccttggcgc gcacttcacc ggcaagcgca atggcgccgc
     2941 cgaacagcag cagcttttcg gtgagcgtgg tcttgccggc gtcagggtgc gagatgatcg
     3001 caaaggtgcg ccggcgcgag acgggatcag gggcagtcga ttgggtcatg gcgtgaggag
     3061 ccggaggata gggcctatgt caagcggcgc agatgccgga aaacgatgat gcgcgagacg
     3121 aacccggtgg tgaccgcgac cgcgccggcg atgagcgcga tcaaggcata acccttcagc
     3181 gagagcgaaa agctgccgaa catggcttcg atctgatcgc cgccggcggt cgcgcgcatc
     3241 aacgccgata tggagctcga cagcagaaaa accaagagcg ccccgccgcc gccgatcagg
     3301 ccgccgcgca agccgaggcg aaagaaatgc cgctgaaact ggcgcgagat gtagccgtcg
     3361 gcagcgccga caagatgcag aacttcgatg atttcgcgat tgcccgccat cgcgccgcgc
     3421 gtcgcgaaac cgacggccag aaccatcgcc agcatcacca gcgcgaaaat gacgaaggcg
     3481 atgacgacca cggtccccgc catcaccgaa aggcgctgta gccacagccg gtgatcgtcg
     3541 accgtcgcat tcggcacttt gtccatcacc gccttgcgca gggcttcgac atcgagcgtc
     3601 cccttcgggt cgagcttcac cacgatgagc cgcggcaccg gcaattcgga gagatcgagg
     3661 cccgtgccaa gccacggcgc gagcaaagcc tcggattgcg ccttgctata ggcatagacg
     3721 tcggcgatgc ccgacgccgt ccgcgcggcg gcaaccgccg cggtcgtatc ggcatcgaga
     3781 tcgcgcccgt caacgggacg gacttgaatc gtcatctcgc gcgccacctg cccgcgccaa
     3841 tcgtcggcgg aatgcgcgat cagcatcgcc gcacccgcgg tcagcccagc gagaaaggtc
     3901 atgatggcca cgacagtaac gagcgcccgc ccagcaattg aggtcgctgg cacgagcggc
     3961 acatccttgc gcatgcccgc agtagcgtca tcggcgaaac gcccgagttt gtgccgctct
     4021 tcggattgca catagatcat gcgcgttcac tcataaatgt gtagccggcc gtcgccgagc
     4081 acgaggcggc gcgcggtctc gaattgatcc atcaacgcca gatcatgcgt ggcgatgaca
     4141 acggcggtgc cggatttgtg caactcggcg aagagccgca aaagccgccg tgccaaagtc
     4201 ggatcgacat tgcccgtcgg ctcgtcggca agcaacagtt ccggccggac gatgagggcg
     4261 cgtgcaatcg ccgcgcgctg tttttcaccg cccgagagca ccggcggtag aacatgcatg
     4321 tgatcgccga ggccgaccca gcgcagcagt tcgacgacct cagcgcgata agcagattcg
     4381 tcgcggccct gcacgcgcag cggcagcgcg acattctcgt aggtcgtcag atggtcgagc
     4441 agccggaaat cctggaaaac gacgccgacg cggcggcgca gatcagtgat ctggtccttg
     4501 ctgagctggg cggaatcccg gttgaacagt gagatcaacc cgcgcgtcgg cttcagcgac
     4561 agaaggatca ggcgcatcag cgtcgtcttg ccggcgccgg agggcccggt cagaaattga
     4621 aacgaatgcg gttcaatctc gaagctgatg tccttcaaga tttcattgcc catcccgtaa
     4681 cgcaggccga cgttctcaaa acggaccaag acaaactccc gcgggcgatg gccgcgcgcc
     4741 gaatcaccgg ctttaactgc gggttaacca taccagccga cgattgcatc atcaggcggg
     4801 aaatgactcg ttccgccgtt cttcaaccgg tttgagccga acgtgctgat cgactgtccc
     4861 ggctgcgctg cctcctacca catcaccaag gcggcgctga gcccgagcgg gcggcgcgtg
     4921 gcctgcccgc gctgcgaaac cgtctggctg gccacgccct gcctgccgga ggtcgcgggc
     4981 ttcattaagg ccgcaccgca tttttcgcca gaagaagagc cgccaccgag gcagacagac
     5041 tatgtccggg ccgttgcggc ccctttgccg cgggcactcc ggccgcgcgg aggcgggttc
     5101 gccggcaagt tcctagtcgg caccgccgcg ctagcactgg ccatgggtct catcgcgtcg
     5161 cgcgacgcca tcgcccgggt atggcccgcc gccgacaggc tttatgccga tatcgggatc
     5221 ccggtcggcc agcgaagcct ggcaatccgc gaccttcaca cggtgctcac gcgcacgaat
     5281 ggcgaggttt ttctcggcgt cgaaggaatc atcttcaatc aacgccaaga agaaacggca
     5341 gtgccgccaa ttcgcctcgc catcctcgat gctgccgggc atgagcttta cacttggcag
     5401 gttgcgcccg cacgtcatgt gctcgcaggc ggcgacagtc tgcccttccg tgcccggctc
     5461 gccgagccgc cgcaggatgg ccgcgacgtc gtcgcgcatt ttgccacgcc ggatgagatc
     5521 gtcgccgatc gctgaagctg gcgcgaaggc cctacacagg ctacgtattt gtggcgaaag
     5581 ctttgccgct gtgcaaagcg ccgggagttc gtgccatggt gcctttgtcc aaattgattg
     5641 gagctgcttc gctttttctc gcgatgacat gcgccttcgc cgcgccatct cttgccg
//
LOCUS       DASWOG010000309         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_354963,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000309 DASWOG010000000
VERSION     DASWOG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354963"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VGB93_06440"
     CDS             complement(<1..451)
                     /locus_tag="VGB93_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY0145776.1"
                     /translation="MSDRAKIFGDIRRALGVTGNEDARRRAVAARLAAAPEGIVPARG
                     RSADPAALFRAEAERVFATVEQVATSAEIPARIADVLRAKNLPPRLRKGDDAYLDALP
                     WTTTTLTLDNGPAQADDAIGLSHSFAGVAETGTLALVSGPDNPTTLNF"
     gene            complement(448..1938)
                     /locus_tag="VGB93_06445"
     CDS             complement(448..1938)
                     /locus_tag="VGB93_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEY0145777.1"
                     /translation="MSAPPDSKNSERDSREKPGSTFSHPALGAADFSANAHRALGDAQ
                     LQHALHNSRDGFILKRAKAVARLPEFEALRDAARDIKAHTLEYLDLYLEAYEEKVLAS
                     GGMVHYAATAEAANSIVLDICRKHGAKSVAKGKSMIGEEMGLNAALEAVGITPVETDL
                     GEYIIQLRHEMPSHIIAPAVHLTKEQIAADFRRAHQALPHDRDLTEPSALLTEARKIL
                     REKFLAADVGITGANFLIAETGTSIIVTNEGNGDLSQSLPKVHIVLASIEKLVPTLED
                     TGELLRVLARSATGQDMSVYTTLSTGPRRPGDPDGPQAYHVILLDNGRTKMLGGNFAE
                     MLRCIRCGACMNHCPVYQSIGGHAYGSVYPGPMGSVLTPGLFGLDHSSDLPNASTLCG
                     ACESVCPVRIPLPKLLRDWRTRAFNRHIGPESQRIGLAFWGFFAKRPALYGLAARIGM
                     WALAFLGRAGKFRSLPLASGWTASRDFPAPQGPTFQAQFSALRKKP"
     gene            complement(1935..>2658)
                     /locus_tag="VGB93_06450"
     CDS             complement(1935..>2658)
                     /locus_tag="VGB93_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0145778.1"
                     /translation="VDLFRPSIGWATVKLLEDAGCRVEVPVQTCCGQPAYNSGDRRLA
                     KDLARPVIEAFASYDYVVVPSGSCAGMLAKHYPALFANDPDFEAEAQSFAAKVYELSD
                     FLVTVLNVTQVDAEFPRRVTYHDACSGLRELGVASQPRELLGSVRGLDLVEMDDHTRC
                     CGFGGTFAVKYGEISAAIVDEKAEAAEKSGAPVLLAGDLGCLMNIAGRLARKGSAIEV
                     RHFAEVLAGMTDQPAICEVRKR"
BASE COUNT          518 a          792 c          867 g          481 t
ORIGIN      
        1 ggaaattgag cgtcgttgga ttgtccggcc cggagacgag cgcgagcgtt ccagtctcgg
       61 cgacgccggc aaaggagtgc gagagaccga tggcatcgtc cgcttgggcc gggccgttat
      121 cgagtgtcag cgtcgtcgtc gtccaaggca aagcgtcaag ataggcgtcg tcgcctttgc
      181 gcagacgcgg cggcagattt ttcgcgcgca ggacatccgc gatccgcgct ggaatttcgg
      241 ccgatgtcgc gacctgttcg acggttgcga acacgcgctc ggcttcggca cgaaagagcg
      301 cggcaggatc ggcggagcgc ccgcgcgcgg gaacgatgcc ctcgggcgcg gctgcaagcc
      361 gcgccgcgac cgcccggcgt cgtgcgtcct cattgccggt gacgccaagc gcacggcgaa
      421 tatctccaaa aatcttcgcc cgatcgctca tggctttttc ctcaaggcgg aaaactgcgc
      481 ctgaaacgtc gggccttgcg gcgccggaaa atcgcggctg gcggtccagc cggaggcgag
      541 aggcaggctg cggaatttgc ccgcgcgccc gagaaatgcc agcgcccaca tgccaatccg
      601 cgcggcgaga ccatagagcg ccggccgctt ggcgaagaag ccccagaacg cgaggccgat
      661 acgctgcgac tctggaccaa tatggcggtt aaaggcgcgc gtgcgccaat cgcgcagcaa
      721 tttcggcagc ggaatgcgga ccgggcaaac gctttcgcac gcgccgcaga gcgtcgacgc
      781 gttgggcaga tcggaagaat gatccagacc gaaaaggccc ggcgtcagca ccgaccccat
      841 gggccccgga tagaccgagc cataggcatg gccgccaatc gattgataga ccgggcaatg
      901 gttcatgcag gcgccgcaac ggatgcagcg gagcatttcg gcaaaattgc cgccgagcat
      961 tttggtgcgg ccattgtcga gcaggatcac atgataggcc tgcggaccat cggggtcgcc
     1021 cggtcggcgc ggacctgtcg agagcgtcgt atagaccgac atgtcctggc ctgtcgcaga
     1081 acgcgccagc acgcgcagca attcgcccgt gtcttcgagc gtcggcacca gcttttcgat
     1141 cgaggcgagg acaatatgca ctttcggcag gctctgcgag agatcgccat taccttcgtt
     1201 ggtgacgatg attgacgtgc cggtctcggc gatgaggaaa ttcgcgccgg tgataccaac
     1261 gtcggcggcg aggaattttt cgcgcaggat tttgcgcgct tcggtcagca gtgcggaagg
     1321 ttctgtcaga tcacggtcat gcggcagggc ttgatgcgcg cggcggaagt ccgcggcgat
     1381 ctgctccttg gtcagatgca ccgccggcgc gatgatatgc gacggcattt cgtggcgaag
     1441 ctggatgata tattcgccga ggtccgtctc gaccggggtg atgcctacgg cttcgagcgc
     1501 agcattgagg cccatttcct cgccgatcat cgacttgccc ttggcgacgc ttttcgcgcc
     1561 gtgcttgcgg cagatgtcga ggacgattga attggcggct tcagccgtcg cggcgtaatg
     1621 caccatgccg cccgaagcca aaaccttctc ctcataggcc tcgaggtaaa ggtcgagata
     1681 ctccagcgta tgcgccttga tatcgcgcgc ggcgtcgcgc aaggcctcga attccggcag
     1741 ccgcgcgacg gccttggcgc gcttgagaat gaatccgtcg cgcgaattat gcagcgcatg
     1801 ctgcaattgg gcgtcgccga gcgcgcgatg cgcgttggcg gaaaaatccg cggcgccgag
     1861 agcgggatgc gaaaaagtgg accccggttt ttcgcgtgaa tcccgctctg agtttttaga
     1921 atctggcggt gcactcatct tttccgcacc tcgcagatcg ccggttgatc ggtcatcccg
     1981 gcaagcactt cggcgaaatg gcgaacctcg atcgcgctgc ctttgcgcgc tagccgtccg
     2041 gcgatattca tgagacagcc gagatcgccg gcgagcagaa cgggtgcgcc gcttttctct
     2101 gcggcctcgg ctttttcatc gacgatcgcg gcggagatct cgccatactt tacggcgaac
     2161 gtgccgccaa agccgcagca acgtgtgtga tcgtccattt cgacaagatc gagcccacgt
     2221 actgagccga gaagttcgcg cggctgcgat gcgacgccca actcgcgcag gccggagcag
     2281 gcgtcatggt aagtaacgcg acgcggaaat tcggcatcga cctgtgtgac gttcaagacc
     2341 gtgacaagaa agtctgacag ttcgtagact ttggcggcga aactctgcgc ctcggcttca
     2401 aaatcagggt cattggcgaa aagcgccgga taatgtttgg cgagcattcc ggcgcaggag
     2461 ccggacggga cgaccacata atcgtaagat gcgaaggctt cgatgacagg ccgcgcgaga
     2521 tctttggcca agcggcggtc gccggaatta taagcgggct gaccgcaaca ggtttgcacc
     2581 gggacttcta cccggcagcc ggcatcttcg agcagtttga cggtcgccca gccgatcgac
     2641 gggcgaaaca gatcgacg
//
LOCUS       DASWOG010000310         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_98879,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000310 DASWOG010000000
VERSION     DASWOG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98879"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VGB93_06455"
     CDS             <1..1205
                     /locus_tag="VGB93_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0145779.1"
                     /translation="LCIYTSGTTGLPKAANVSHRRIMTWSYWFAGILNTSPADRTMNC
                     LPMYHSVGGIVAIGSVLVSGGSVVLQEKFSTRRFWDDVVRWDCTLFQYIGELCRYLLN
                     APENANERAHRLRLAFGNGLRADIWSAFRERFNIPHIVEFYAATEGTFSLINVEGEPG
                     AVGRVPAFLKHRFPSALVKFDVVRGEPFRDSDGFCVPCAPDEIGEAIGKIASGASAGA
                     RFEGYTSAKDSDAKVLRNVFAAGDAWFRTGDLMRQDAKGFFYFVDRIGDTFRWKGENV
                     STFEIAEALNAVPGVTDATAYGTTVPGADGRAGMATLVIAPGFDLKLLHGRLSALLPS
                     YARPLFIRISAEIEVTETFKQKKAQLAREGFDPSKIEDQLYFDNGTGYVPLDGSLFAR
                     ITAGEIRL"
     gene            complement(1180..>1767)
                     /locus_tag="VGB93_06460"
     CDS             complement(1180..>1767)
                     /locus_tag="VGB93_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEY0145780.1"
                     /translation="EHPFPAALDDILAALEWIFERPSAYGVERIGVAGDSAGATLAAA
                     AAAEWGKQKKPDLAFQLLFCPIFDFAGETPSRHAFASPVLDQATLDHDIALYTAGKVG
                     ADDPRVSPLRATDFSGLPPTFLHTAQCDPLRDEGAAYAHKLRAAGVEVHHTCHAGMPH
                     LFYALSGVIPAAKAAAAAIGAELSAWLATSESRRR"
BASE COUNT          341 a          539 c          549 g          338 t
ORIGIN      
        1 cactctgcat ctacacatcg ggtacgaccg gcctgccaaa ggcggcgaat gtcagccacc
       61 ggcggatcat gacctggagc tattggttcg ccggaattct gaacacaagc cccgccgatc
      121 ggacgatgaa ttgcctgccg atgtatcaca gcgtcggcgg catcgtcgcc atcggcagtg
      181 tcctcgtcag cggcggctcg gtcgtgctgc aagagaaatt ttcgacacgg cgcttctggg
      241 acgatgtcgt gcggtgggat tgcaccctgt ttcaatatat cggcgaactc tgccgctatc
      301 ttctcaacgc gcctgaaaac gccaacgaac gggcgcatcg gctgcggctc gccttcggga
      361 acggcctgcg cgccgatatc tggagcgcgt ttcgagagcg cttcaacatc ccgcatatcg
      421 tcgaatttta tgcggcgacc gagggcacgt tttcactgat caatgtcgag ggcgagcccg
      481 gcgcggtcgg ccgcgttccc gcctttctga agcatcgttt tccgtccgca ttggtgaaat
      541 tcgacgtcgt gcggggtgag cccttccgcg attccgacgg cttctgcgtg ccctgtgcgc
      601 ccgacgagat cggcgaagcg atcggtaaaa tcgcgtccgg cgccagcgcc ggggcgcgat
      661 ttgagggcta taccagcgcc aaagacagcg acgcgaaggt tctgcgcaat gtttttgctg
      721 cgggcgacgc ctggtttcgg accggcgatc ttatgcggca ggacgcaaaa ggctttttct
      781 atttcgtcga tcggatcggt gacacgttcc gctggaaagg cgagaacgtc tcgacgttcg
      841 agatcgcgga ggcacttaac gccgttcctg gtgttaccga tgcgaccgcc tatggcacga
      901 cggttcccgg tgcggacgga cgcgccggca tggcgacgct cgtgatcgcg ccgggattcg
      961 atctgaagct cctgcacggg cggctcagcg cgctgttgcc ttcttatgcg cgcccgctct
     1021 ttatacggat cagcgcagag atcgaggtca cggaaacctt caaacagaag aaagcccagc
     1081 tcgcacgcga gggcttcgat cccagcaaga tcgaggatca gctctatttc gacaacggga
     1141 ccggctatgt gccgctcgac ggctcgcttt tcgcgcggat caccgccggc gagattcgct
     1201 tgtagccagc cacgccgata actcggcgcc gatcgccgcc gcggcggcct tcgccgcagg
     1261 aatgacgccc gatagagcgt agaaaagatg tggcatgccg gcatggcatg tatgatgaac
     1321 ttcgacgccc gccgcacgga gtttatgggc ataggccgca ccttcatcgc gcaacgggtc
     1381 gcattgcgcc gtatgtagaa atgtcggcgg caggccggag aaatccgtcg cacgcagcgg
     1441 agagacgcgc ggatcgtcgg cgccgacctt acccgcggta taaagcgcga tatcgtggtc
     1501 gagcgtcgcc tgatcgagga ccggtgacgc gaacgcatgg cgcgacggcg tttccccggc
     1561 gaaatcgaaa atgggacaaa acagaagctg gaaggccaag tccggctttt tctgcttccc
     1621 ccattcggcg gcagcggcgg cggccagtgt cgcgcccgcc gaatcaccgg cgaccccaat
     1681 tcgctcgacg ccataagctg atggcctttc aaaaatccat tcgagcgctg ccagaatatc
     1741 gtccaacgcc gcgggaaaag gatgttc
//
LOCUS       DASWOG010000311         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_80104,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000311 DASWOG010000000
VERSION     DASWOG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80104"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..821
                     /locus_tag="VGB93_06465"
     CDS             6..821
                     /locus_tag="VGB93_06465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0145781.1"
                     /translation="MSEEMPREALTQLLRWYADMGVDLAVDDMPHDRFAESQSPEKAA
                     PPAVNPPRAVAQPARVAAPAPVRITAPPLRTEDLVLEAARAETLEDLRAQLLAFEGCG
                     LKGTATQLVFSDGNPEADIMLVGEAPGADEDRIGRPFVGRAGQLLDKMLATIGLDRTQ
                     VYIANVVPWRPPGNRTPTPLETGACLPFTRRQIELVAPKVLICLGAPSMQTLLCIKEG
                     IMRSRGRWVDYNCGGTIIPAMAMLHPAYLLRQPSHKKFAWQDLRELAKKLRDV"
     gene            905..1837
                     /locus_tag="VGB93_06470"
     CDS             905..1837
                     /locus_tag="VGB93_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY0145782.1"
                     /translation="MEWQNFRRSDNVEDRRGDSDSGDPAGGGGGLSLPGGLGAGHLGL
                     GAIIIIGLIGWALGIDPRVLIGGAEMLNGGQSTQQAPASPAPTQQASTETGVPKDQIG
                     QFVAAILAETEDVWSQVLPAQKGIQYVPPKLVLYNGFTRSGCGGASASMGPFYCPEDK
                     KVYLDTSFFRDMQTKYGGGGDFAYAYVIAHEIGHHVQDQLGILSKVNAAEQNADDQTQ
                     ANALSVRIELMADCLAGVWANNAQAKYHVIDQGDVEKAVATAQAIGDDRLQKATRGYA
                     VPDSFTHGTAAQRVQWLETGLKSGQIDACNTFAQ"
     gene            complement(1847..2377)
                     /locus_tag="VGB93_06475"
     CDS             complement(1847..2377)
                     /locus_tag="VGB93_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0145783.1"
                     /translation="MISLIVLCSSPAAASGDSPAAEAIVRTLSAFVGAAVRGLVREAV
                     LVGPPHIKLGYIADHAGCAFIESKSEAEGIADALPLARAAHLLIVRAGYVPETGFAEA
                     IEDLLRFGKAETQGWLLRGEAERPLEKILAHLTRPAALLAPRRLCLGVKSPSLAALAK
                     VTRARPARHIRLRRVI"
BASE COUNT          504 a          821 c          839 g          479 t
ORIGIN      
        1 cggccatgtc tgaggagatg ccgcgcgagg cattgacaca actcctgcgc tggtatgccg
       61 acatgggcgt cgacctcgcg gtcgacgaca tgccgcatga tcgtttcgcg gaatcgcaaa
      121 gcccggaaaa ggctgcgccg ccggccgtaa atccgccgag ggccgtcgct cagcccgcgc
      181 gggtcgcagc acctgcgcct gtgcgtatca ctgcaccgcc gctgcgcacc gaagatctgg
      241 tgctggaggc cgcgagagcc gaaacgctag aggatcttcg tgcgcagctc ctcgccttcg
      301 agggttgcgg cctgaaaggg accgccacgc agctcgtttt ctccgacggc aatcccgaag
      361 ccgacatcat gctggtcggc gaggcgccgg gcgccgatga agatcgcatc ggccggccat
      421 ttgtcggccg cgccgggcaa ttgctcgaca agatgctggc gacgatcggg ctcgaccgca
      481 cgcaagtcta tatcgccaac gtcgtgccct ggcggccgcc cggtaatcgg acgccaacgc
      541 cgctcgaaac cggcgcatgt ctgccgttca cgcgccggca gatcgaactt gttgccccga
      601 aagtcctgat ttgcctcggc gcgccatcga tgcagacgtt gctctgcatc aaggaaggta
      661 tcatgcgcag tcgtggccgc tgggtggatt ataattgcgg cggcacaatc attccggcaa
      721 tggcgatgtt gcatccggcc tatttgctgc gccagccgtc gcacaagaaa ttcgcctggc
      781 aggatctgcg cgaactcgcc aagaagcttc gcgacgtctg agcacttcgt gacctggcgc
      841 acagtcaagg tttgcccgct tctagaacgt gctagactgg tacgaaagcg agggagcgcg
      901 cgcgatggaa tggcagaatt ttcgccggtc ggacaatgtt gaggaccggc gcggcgacag
      961 tgattctggc gatcctgcgg gcggtggtgg tggtttgtct ctgccgggcg gacttggcgc
     1021 gggccatctg ggcctcgggg ctatcatcat tatcggactg atcggctggg cgctcggcat
     1081 tgatccgcgc gttctcatcg ggggcgccga aatgttgaac ggcggccaga gcacgcagca
     1141 agcccccgcg tccccggcgc cgacgcaaca ggcttcgacc gaaaccggcg tgccgaaaga
     1201 tcagatcggc cagttcgtcg ccgccattct tgcagagacg gaagatgttt ggtcgcaagt
     1261 cttgccggcg cagaagggca ttcaatacgt tccgcccaag ctcgttctct acaacggctt
     1321 cacacgttca ggctgcggcg gcgcctcggc ctcgatgggg ccgttctatt gtcctgaaga
     1381 caagaaggtc tatctcgaca catccttctt ccgtgacatg cagacgaaat acggcggcgg
     1441 cggcgacttc gcttatgcct atgtgatcgc gcatgagatc ggccaccacg ttcaggatca
     1501 gctcggcatc ctctcgaagg tcaacgcggc cgagcagaat gccgacgatc agacgcaggc
     1561 caacgcgctc tccgtgcgga tcgaattgat ggccgattgt ctcgccggcg tctgggccaa
     1621 caacgcgcag gcgaaatatc acgtcatcga tcagggcgac gtcgagaagg cagtcgcgac
     1681 ggctcaggcc atcggcgatg atcggttgca gaaggcgacg cggggctatg ccgtgccgga
     1741 ttccttcaca cacgggaccg cggcgcagcg cgtgcaatgg ttggagacag ggctgaaatc
     1801 gggccagatc gacgcctgca atacgtttgc ccaataggct ttaagcctaa atcacccggc
     1861 gcagcctgat atgacgcgcg ggtctggccc gcgtcacctt ggcaagtgcg gcgaggcttg
     1921 gcgactttac gccgaggcag agccgccgcg gcgccagcag tgccgctggc cgggtgagat
     1981 gagccaggat tttctcgagc ggccgttcgg cctcaccgcg caacagccag ccctgcgtct
     2041 cggccttacc aaagcgtaga aggtcttcga tggcctcggc gaatccagtt tcgggcacat
     2101 agccggcgcg aacgataagc agatgcgccg cacgagcgag gggcagggca tcggcgatcc
     2161 cctcggcttc cgatttagat tcaatgaaag cgcaaccggc atggtcggcg atatagccga
     2221 gcttgatgtg cggcggtccg acgaggaccg cttcgcgcac gaggccgcgg accgccgcgc
     2281 caacaaaggc cgacagcgtg cgcacgatcg cttcggcggc cggagagtcc ccgctggcag
     2341 ccgcaggcga cgaacagaga acgatcaggg aaatcattgc gagctcttaa acctgttccg
     2401 gattttgcgc gtcggcaatt gacacgatgc gttttgttcc ataaatgttc tcatcatgtt
     2461 cttacattaa ggcgtcgcaa atggccgcca aactcatgtc tcgagccacc aaggttcaag
     2521 caaaatctac cgttcctggc gagggtgggc gccgtgtagc ccaagcggtg ccgccctcgc
     2581 ttcggccgcc gcgcgtcttc gattcacccc ccggaatcga ggacgcgcgg cctcttatgc
     2641 cgc
//
LOCUS       DASWOG010000312         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_25386,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000312 DASWOG010000000
VERSION     DASWOG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25386"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="ligD"
                     /locus_tag="VGB93_06480"
     CDS             complement(<1..1727)
                     /gene="ligD"
                     /locus_tag="VGB93_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0145784.1"
                     /translation="MALEAYHKKRNFDVTSEPRGAKGRGSGHSFVVQKHDATRLHYDF
                     RLELDGVLKSWAVTRGPSLVPGEKRLAVHVEDHPLDYGGFEGTIPEGQYGAGAVIVWD
                     RGTWTPDGDPHAGYAKGHLDFVLHGQKLKGRWHLVRMHPRPGEKGDNWLLIKGDDEEA
                     RGPRDPDILKEEPNSVVSGRSIDDIAGDKKSRRWTSGRAAQGRIETLGSPRARALAPL
                     DSVVKSDPASAKRRSAKPAKADEPAGKPASKPAGRKTSGYAFTPPKGAKRKAQPDFLP
                     PMLPTLAAAPPSGTQWIHELKFDGYRIEAVAKAGVVRLRTRTGLDWTDRFPTIAVAIA
                     ALAGREFIIDGEVVVEEENGVSDFSALQNALSEGDASGMVYYAFDLLAIDGYDLTALP
                     IVERKETLAALLMTAPKDTILRLSEHFDENGDLMLQHVCRLGAEGIVSKKRDSAYISK
                     RTTEWIKTKCANRQEFVIAGFVPSTTARKAIGSLVVGYYDQGELRHAGRVGTGYSNKM
                     AADLFADLSREKVAKPAFSAPLSAEARRNAVWVKPTRVAEVEFRGWTADANLRQASFK
                     GLREDKDPK"
     gene            complement(1742..2626)
                     /locus_tag="VGB93_06485"
     CDS             complement(1742..2626)
                     /locus_tag="VGB93_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0145785.1"
                     /translation="MAPRANWKGYLKLSLVSCPVALFPAVSRTERISFHLINRETGNR
                     LRQQYIDSVTGDIVERDEMVRGYEIGKNDYITLDETEIAEQAIESTHTIDIETFVPRD
                     EIDEVYLDGSYYLAPDDKVADEPFVVIRDAMRKSNMVGLARVVLSGRERIIMIEARDS
                     GLLATTLHYKYEVRDEKPYFESIPDVKLSKEMLDLAAHIIDTKRGKFDPSKFEDRYQD
                     SLVDLIRAKRAGKPLPAAPQPKTPSNVINLMDALRRSVDAESGRRGTGKTSAKAHSAA
                     KKAAPAKSATKRRVSKAS"
     gene            2971..3228
                     /locus_tag="VGB93_06490"
     CDS             2971..3228
                     /locus_tag="VGB93_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0145786.1"
                     /translation="MSGDSLLIILVVGVVAGWLAGQILRGTGFGLVADLCIGIIGAFI
                     GNWILPKLGIHLGAHLLPQIISATIGAIVLLLILGLFRRRY"
     gene            3322..>3850
                     /locus_tag="VGB93_06495"
     CDS             3322..>3850
                     /locus_tag="VGB93_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0145787.1"
                     /translation="MADFFQTAFVTLLVTLDPPGLAPIFIALTLGMTKIEKREVAVRA
                     STIAFAILFIFALGGATVMATLGISLPAFRIAGGLLLFYTAFQMIFAERGQRKKELAT
                     DAVTIDHVNNLAAFPLAMPLMSGPGSITAVILLAGRASGHWREQTGLILIILGVILMC
                     YIVFLISEQVARIFGT"
BASE COUNT          689 a         1213 c         1112 g          836 t
ORIGIN      
        1 tctttcgggt ctttatcttc gcgcaaacct ttgaacgaag cctggcgcaa attcgcatcc
       61 gccgtccagc cgcgaaattc gacctccgcg acccgtgttg gtttgaccca cacggcattg
      121 cggcgtgctt ccgccgagag cggtgcgctg aacgcgggct tcgcgacttt ttcacgcgat
      181 agatcggcaa aaaggtcggc agccattttg ttggaatagc ccgtgccgac gcgtcctgcg
      241 tgtctaagct cgccctggtc gtaatagcca accaccagtg aaccgatcgc cttgcgcgcc
      301 gtcgtcgagg ggacaaagcc cgcgatgacg aactcctgcc ggttggcgca ttttgtcttg
      361 atccattcgg tggtgcgttt ggaaatgtag gcgctgtcgc gcttcttcga gacgatgcct
      421 tccgcgccga ggcggcagac gtgctgcagc atcaggtcgc cattctcgtc gaaatgttcg
      481 ctcaagcgca gaatggtgtc cttcggtgcg gtcatcagta gcgccgccag agtctccttc
      541 cgttcgacaa tggggagcgc cgtgagatcg taaccgtcga tagcgagaag gtcgaaggcg
      601 taatagacca tgccgcttgc atcgccttca ctcagcgcat tctgcaaggc cgagaaatcc
      661 gagacgccgt tttcctcctc aacgacgact tcgccatcga tgatgaactc cctgccagcg
      721 agcgccgcga tcgcaactgc gattgtcgga aagcgatccg tccaatcgag gccagtgcgt
      781 gtccgcagtc tcaccacgcc agctttcgcc acggcttcga tgcgatagcc gtcgaatttc
      841 agctcgtgaa tccattgcgt acctgaaggc ggcgccgctg cgagcgttgg gagcatgggc
      901 gggagaaagt ccggctgcgc cttgcgcttt gcgcccttgg gcggcgtaaa agcataaccg
      961 ctggtctttc tgccagccgg cttgctcgcc ggcttgcccg ctggctcgtc ggctttcgcg
     1021 ggcttcgccg aacgccgctt cgccgaggcc ggatcggatt tgacgacact atccagcggc
     1081 gccagcgcac gcgcacgtgg cgaacctagc gtctcgatcc gtccctgtgc cgcccggccc
     1141 gacgtccagc gacggctttt cttgtcgcca gcaatgtcat cgatcgagcg cccgctaacg
     1201 accgaattgg gctcttcttt cagaatatcc ggatcgcgcg gtcctcgcgc ctcttcgtcg
     1261 tcacccttga tcaggagcca attgtcaccc ttctcgcccg gccgcggatg catacggaca
     1321 agatgccagc ggcccttcag cttttggccg tgcaaaacga aatcgagatg gcccttggca
     1381 tagccggcat gcggatcgcc gtccggcgtc caagtcccgc gatcccagac gatcacggcg
     1441 ccggcgccat attgcccttc ggggatcgtg ccttcgaagc ccccgtaatc gagcggatgg
     1501 tcctcgacat gaacggcgag gcgcttttcg cccggcacca ggctcggccc gcgcgtcacc
     1561 gcccagctct tcagcacgcc atcgagttca agccggaaat cgtaatgcag gcgcgtcgcg
     1621 tcatgtttct gcacgacaaa cgaatgaccg ctgccgcggc ccttggcgcc gcgcggctcc
     1681 gacgtgacgt caaaattgcg ctttttgtga taggcctcga gcgccatgga agccccgcct
     1741 ttcagctcgc cttgctgaca cgccgcttcg ttgcggactt cgcaggcgcc gctttcttcg
     1801 cggcgctatg cgcctttgcg gacgttttgc ccgttccgcg ccggccggat tcagcgtcga
     1861 cgctacggcg cagcgcatcc atcagattga tgacattgct cggcgtcttc ggctgcggcg
     1921 ctgccgggag cggcttgccg gcgcgcttgg cccggatcag atcgacgagc gaatcctggt
     1981 agcgatcctc gaatttcgac ggatcgaact tgccgcgctt ggtatcgatg atgtgcgcgg
     2041 caaggtcgag catctccttc gagagtttga cgtcgggaat cgattcgaaa tagggctttt
     2101 catcgcgcac ttcatatttg tagtgcagcg tcgtcgcgag caggccactg tcacgcgcct
     2161 caatcatgat gatgcgctcg cggccggaca gcactacgcg cgcgaggccg accatgttgc
     2221 tcttgcgcat cgcgtcgcga atgacgacga aaggctcgtc ggcaactttg tcgtcgggcg
     2281 caagataata agagccgtcg agatagactt cgtcgatttc atcgcgcggc acgaaggtct
     2341 cgatgtcgat cgtatgcgta ctctcgatcg cttgctcggc gatttcagtt tcatcgagcg
     2401 tgatgtaatc gttcttgccg atttcgtagc cgcgaaccat ttcgtcgcgc tcgacgatgt
     2461 cgcccgtcac ggaatcgata tattgctgtc gaagccggtt gccggtttca cggttgataa
     2521 ggtgaaacga aatgcgctcc gtccggctga ccgccgggaa aagcgcaacg gggcaggaaa
     2581 ccagcgagag cttgagatag cccttccaat tcgcacgcgg tgccacagct gagaccctcg
     2641 gtactgaccg gaagcgggcg gcccgctccc gactacgcta aactacgacc attacaccac
     2701 ataagcccaa gcaacgccac gcgaatgaaa gcgcctaaga cgcatagtgg agccaacgtc
     2761 aatcatgcta aaatgttccg tggcgtctgc cgcgtgaggg ggccgggcgc ccccgcaggg
     2821 tttaatcatc cggcggtatc gcgccgcagc aacggacacg ctacgactac ttagcgatgt
     2881 gtgttaatca ttcgtgcggc ggggcacacc gctatctgcg tcctgcccat aggacggctt
     2941 atcgttcact cacatgaaga gggatttgcc ttgtctggag acagtctcct gatcattctc
     3001 gttgtcggcg tcgtcgctgg ctggctcgcg ggccagattt tgcgcggcac tggcttcggc
     3061 ctcgtcgccg atctctgcat cggcattatc ggcgcgttca tcggcaactg gatattgccg
     3121 aagcttggca ttcatcttgg cgcgcatctg ctgccgcaga tcatctccgc gacaatcggt
     3181 gcgatcgtgc tgctcctgat ccttggcttg ttcagacggc gctattgaac taagctccag
     3241 ccatgccttc gagccgcggc gcacacatga gcgtcgcggc ttttgcatgt gtaacccccg
     3301 tgtcgcgacg gaaacgaagc catggcagac ttctttcaaa cggccttcgt cacgctgctc
     3361 gtgactttgg acccaccggg gctcgcgccc atctttatcg ccttgaccct gggcatgaca
     3421 aagatcgaaa aacgcgaagt cgcggtccgc gccagcacca tcgctttcgc cattcttttc
     3481 atatttgcac ttggcggcgc caccgtgatg gcaacgcttg gcatttccct gccggcgttc
     3541 cgaatcgcag gcggccttct gcttttttat acagcgtttc agatgatttt cgccgagcgc
     3601 ggccagcgaa agaaggaact cgcgaccgat gcggtgacga tcgaccacgt caacaatctc
     3661 gccgcctttc ccctcgcaat gcctctgatg agcggccccg gttcgatcac cgcggtaatc
     3721 ctgctcgccg gccgggcgag cggccattgg cgcgagcaaa ccggccttat cctcatcatc
     3781 cttggggtga ttttgatgtg ctacatcgtg ttcctgattt cggagcaagt ggcgcggatc
     3841 ttcggcacaa
//
LOCUS       DASWOG010000313         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_117064,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000313 DASWOG010000000
VERSION     DASWOG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117064"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="ccmI"
                     /locus_tag="VGB93_06500"
     CDS             complement(<1..823)
                     /gene="ccmI"
                     /locus_tag="VGB93_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY0145788.1"
                     /translation="MLWSLFALLTGGAVFAILWPLARPGRRDSTAPDVAFYQAKLEEI
                     ARDEARANISAEEAAAAKTEAARQLLAVSGKDPHSSDAMVQSPKWHLRVAAVIALVFV
                     PAIGFGFYTYLGHPTWPDLPLQARLNAPVGRMDVNAAIARVEAHLAQHPQDGLGYQVL
                     AQALLITGRVGDAVNAAQTAVRLRPQNADTLTVYGETLVVASNGIVTEDARHQFEKAF
                     ASDPHEAKAMFFLGLAASQENDVVTARKYWGPLLASAPPNAPWRADLVARLGQLDK"
     gene            complement(969..>1564)
                     /locus_tag="VGB93_06505"
     CDS             complement(969..>1564)
                     /locus_tag="VGB93_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145789.1"
                     /translation="SVIINEADTEKGPLSSKVHEQAAIMRDQVTYYLDRARAAVRVGA
                     IGNMAEVAPATMGLVRTFEKIYRERGINFSAQVADNIRFRGERQDLEEMIGNLVDNAG
                     KWARQNVTIAITALGANSQGKKFFRVVIDDDGVGLAPELREAAMRRGRRLDETRPGSG
                     LGLSIVADLAALYGGALRLDASPAGGLRAELELPAAA"
BASE COUNT          307 a          499 c          452 g          306 t
ORIGIN      
        1 ccttatcaag ttgcccgagc cgcgcgacga gatcggcccg ccaaggtgcg ttgggcggcg
       61 ccgacgctaa aagcggtccc caatatttgc gcgccgtgac aacgtcgttt tcctgcgagg
      121 ccgcgaggcc aaggaagaac atggctttgg cctcgtgcgg atcggaggcg aatgcctttt
      181 cgaactggtg acgcgcgtct tccgtcacga tgccattcga cgcaacgacg agcgtttcgc
      241 catagacggt cagcgtatcg gcattttggg ggcgcagacg aaccgccgtc tgcgcggcat
      301 tgaccgcatc gcccacgcgc ccggtgatca agagagcttg ggccagaacc tgatagccga
      361 gaccatcctg cggatgctgc gccagatggg cttcaacccg ggcgatggcg gcattgacgt
      421 ccatgcgtcc gactggtgcg ttgagccgcg cctgcagcgg caaatccggc cacgtcggat
      481 gaccgagata tgtatagaat ccaaacccga tcgccggaac aaagacgagc gcgataacgg
      541 ccgcaacccg caaatgccat ttcgggcttt gtaccattgc atcggatgag tgcgggtctt
      601 tcccgctgac ggcgaggagt tgccgtgccg cctccgtctt ggctgcggcg gcttcctccg
      661 cagaaatatt agcgcgcgct tcatcgcgtg cgatctcttc gagcttcgcc tgatagaagg
      721 caacgtccgg ggctgtcgag tcgcgccgcc ccggccgcgc cagcggccag agaatggcga
      781 agactgcgcc gccggtcaaa agcgcgaaca aactccagag catgattttc cttgtgcctc
      841 ttccggctgg gtcagtacgc ctttttcgcc ggaatttcca tccgacaaag gcgtgaacac
      901 ttcgaaccga taaaaaatag atttatgtat tttatcatgg tgttgcatct gactcttaaa
      961 cggtcaattc aagctgccgc cggcaattct agttctgcac gcagcccgcc cgccgggctc
     1021 gcgtcgagcc gcagcgcgcc gccatagaga gcggcaaggt ccgccacgat ggaaagaccg
     1081 aggcccgatc cgggccgcgt ttcatccaat cggcggccac gccgcatcgc ggcctcgcgc
     1141 aattccggcg cgagcccaac gccgtcatca tcgatgacga cgcgaaagaa tttcttacct
     1201 tgcgaattgg cgccgagcgc cgtgatcgcg atggtcacat tctggcgcgc ccatttcccg
     1261 gcattgtcga cgagattgcc gatcatttcc tcgagatcct gccgctcgcc gcgaaaacgg
     1321 atgttgtctg cgacctgcgc cgagaaattg attcccctct cgcgatagat cttctcgaat
     1381 gtacgcacca gccccatcgt cgccggcgca acctccgcca tattgccgat ggcaccgaca
     1441 cgcacggcgg cgcgcgcccg gtcgagataa taggtcacct gatcgcgcat gatcgccgcc
     1501 tgctcatgaa ccttggacga cagcggtcct ttttcagtat ccgcctcatt gatgataacg
     1561 ctga
//
LOCUS       DASWOG010000314         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_336740,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000314 DASWOG010000000
VERSION     DASWOG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336740"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="VGB93_06510"
     CDS             <1..>1570
                     /locus_tag="VGB93_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0145790.1"
                     /translation="TTRADGAYYEHRFFLFSFLFTAAFGMSCYVLIQYPGAQAIANGA
                     AVGYAMGVVARNAGRPSIVITQVMLMIVPMIIGFGSMHNTYGNATAILLVGTIITTLA
                     VTVSLHKNVIAVYNADKATQHLAMYDSLTGLMNRYTFGEQIAHAIATQPNEGFAILYI
                     DLDRFKDINDTLGHTAGDAVIIEVARRLRAVTRGGDFIARFGGDEFLVKISGADLVDT
                     SRIVERIVIALTQPQSVDGKVFAPSASIGVALFPENGQIAGDIIKKADIALYEAKRAG
                     GKTFRIFASDMEHDLHAQRALRSEIELAVERHEFFLHYQPIYELGSRNIIAVEALLRW
                     NHPTRGLLRPDAFIAVAEETRAIIDIGEQVLDAACRMAVRLPEHIAVAVNLSTIQFCQ
                     PERLIAAVRGSLQRAGLRPNRLHLEITESLLLANTPSARKTLDTLTGTGAKLALDDFG
                     TGYSSLSYILDFPFSKIKIDKKFTSSLYCNTASPAIIKAVAQLAGDLSLEVVVEGIET
                     REQEAFVQTLGPTHG"
BASE COUNT          316 a          483 c          437 g          334 t
ORIGIN      
        1 ttactactcg cgcggacggc gcctattacg agcaccgctt ttttctcttt tcgttcctgt
       61 tcaccgccgc tttcggaatg agttgttacg tactcatcca atatcccgga gcgcaagcca
      121 tcgccaatgg tgctgccgtc ggctacgcca tgggtgttgt cgcccgcaac gccgggcgcc
      181 cgagcatcgt gatcacgcag gtcatgctga tgatcgtccc gatgatcatc ggtttcgggt
      241 cgatgcacaa cacctatggc aacgcgaccg ccatactgct ggtggggaca atcatcacca
      301 cgctcgccgt gaccgtctcc ctgcataaga atgtgatcgc cgtctacaat gcggacaagg
      361 cgacgcaaca cctcgcaatg tacgacagcc tcaccgggct catgaaccgt tacacattcg
      421 gcgagcagat tgcgcatgca atcgcgacgc agccgaatga agggttcgcg attctctata
      481 ttgatctcga ccgattcaag gatatcaacg atacgctggg tcacacagcc ggcgacgccg
      541 tcattatcga ggtcgcacgg cggctgcggg cggtcacgcg cggtggcgat ttcatcgccc
      601 gcttcggcgg cgacgaattt ctcgtcaaga ttagcggcgc ggacttggtg gataccagtc
      661 gcatcgttga gcgcattgtc atagctttga cccagccgca aagcgtcgac ggcaaggtct
      721 ttgcgccttc ggccagcatc ggcgtcgcgc tcttccctga gaatggacag atcgccggcg
      781 atattatcaa gaaagccgat atcgcgcttt atgaagccaa gcgcgccggc ggcaagacat
      841 tccgcatttt cgcgtctgac atggagcatg atttgcacgc gcaacgtgcc ctgcgcagcg
      901 agatcgagct cgccgtcgag cggcatgaat tctttctcca ctatcagccg atttacgagc
      961 ttggctcccg gaatatcatc gctgtggaag ctctgctccg gtggaatcat ccgacacgcg
     1021 ggttgctgcg gccagacgcc ttcattgcgg ttgcggagga gacacgggca atcatcgaca
     1081 tcggcgagca ggtgcttgac gcggcgtgcc gaatggctgt gcggctgccc gagcacatcg
     1141 ccgtcgcggt caatctttcg acgattcagt tttgccagcc cgagcgcctc attgctgcgg
     1201 tccgcggcag cctgcaacgc gccgggctgc gcccgaaccg tttgcatctt gaaatcaccg
     1261 aaagtcttct gctggcgaat acgccatcgg cacgcaagac gctcgacacg ctcacaggca
     1321 cgggggccaa actggcgctc gacgattttg gcaccggcta ttcttccttg tcttacatcc
     1381 tggattttcc gttttccaag atcaagatcg acaagaaatt caccagcagc ctttactgca
     1441 acaccgcgtc accagcgatc atcaaggctg tcgcgcagct tgccggcgat ctatcgctcg
     1501 aagtcgttgt cgagggcatc gaaacgcggg aacaggaagc tttcgtccaa accctcgggc
     1561 cgacacatgg
//
LOCUS       DASWOG010000315         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_336785,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000315 DASWOG010000000
VERSION     DASWOG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336785"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGB93_06515"
     CDS             complement(<1..597)
                     /locus_tag="VGB93_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0145791.1"
                     /translation="MSRTDEPRKSRLPESAPPDAAEVSHSDYEPPATVEATPSMAQYL
                     EIKAANPDSLLWYRMGDFYELFFEDAVTASEALSIVLTKRGKHKGQDIPMCGVPVHRA
                     DEYLQRLIKQGYRVAVCEQLEDPAEARKRGAKAVVKRDVVRLVTPGTLTEEALLDAKA
                     RNYLTAVFLATPSELGSATADARVALASLDISTGEFEVA"
     gene            796..>1583
                     /locus_tag="VGB93_06520"
     CDS             796..>1583
                     /locus_tag="VGB93_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY0145792.1"
                     /translation="MASLHRAAHVTAQEALAFHANGKPGKLEITPTKPLATQRDLSLA
                     YSPGVAIPVQAIAENPRDAYAYTSKGNFVAVVSNGTAILGLGNLGALASKPVMEGKAA
                     LFKRFADIDAIDLCVDTPDPDAFINSVRYLGPSFGGMNLEDIKSPDCFVIEEKLKGLL
                     DIPVFHDDQHGTAIVVAAGVLNGLRVVEKDIGDIKLVCAGAGAAALACLNLLVKIGIK
                     RENIYVCDIEGLVYEGRKKLMDEYKAPFAQKTNARTLADVIGGA"
BASE COUNT          327 a          474 c          452 g          330 t
ORIGIN      
        1 agctacctcg aattcaccag tcgagatatc gagagaggca agcgccacgc gcgcatctgc
       61 cgttgcgctt ccgagctccg atggagttgc gaggaacact gctgtcagat agttgcgagc
      121 cttggcgtcg agaagggctt cttcggtcag cgtgcccggc gtcacaaggc gaacaacatc
      181 acgcttgacg acggccttgg cgccgcgctt tcgagcttcg gcgggatctt caagctgttc
      241 gcagacggcg acgcgataac cttgcttaat caaacgttgc aagtattcgt cggcgcgatg
      301 caccgggacg ccgcacatcg gaatgtcctg gcccttgtgc ttgccgcgct tggtcagaac
      361 aatcgacaga gcttccgatg cggtaaccgc atcttcaaaa aatagttcgt aaaaatctcc
      421 catccgatac caaagcaggc tatcgggatt tgctgccttg atttcgagat actgcgccat
      481 cgacggcgtc gcctcgaccg tggccggcgg ttcataatca ctatgcgaca cttcggctgc
      541 gtctggcggc gccgattcgg gcagcctgct cttgcgcggc tcgtcggttc tggacatcaa
      601 gagattacgt ttcaatttgc ctcattcggc ctgatcgatg acgcaatgcg aaatgcccca
      661 ccacccgatc caagcatcgg atatgcatag gttgccgtcc cgcctcccgc aacctatcgt
      721 gccgccgctt atcccctgat gtgtgcttcg gcagtaatgc caacggcacg caaaatccgt
      781 gaatggtccg acaacatggc ctccctccac cgcgcagccc acgtgacggc tcaagaagcg
      841 cttgcgttcc acgcgaacgg caaaccgggc aaactcgaga tcacgccgac gaagccgctg
      901 gcgacgcagc gagatctgtc gctcgcctat tcgccgggtg ttgcaattcc ggttcaggcg
      961 atcgcggaaa atccgcgcga cgcctatgcc tatacgagca agggcaattt cgtcgccgtc
     1021 gtatcgaacg gcacggccat tctcggcctc ggcaatcttg gggcattggc ttcgaagccg
     1081 gtgatggagg gcaaagccgc tctcttcaag cgtttcgccg atattgacgc gatcgatctc
     1141 tgcgtcgata cgccggaccc cgacgcattc atcaattcag tccgatacct cgggccgtcg
     1201 ttcggcggta tgaacctgga agatatcaag tctcccgatt gcttcgtcat cgaagagaag
     1261 ctcaaggggc tgctcgacat cccggtcttt catgatgatc agcacggcac cgcgatcgtc
     1321 gtcgcggcgg gcgttctcaa tggactgagg gttgtcgaaa aggacattgg ggatatcaag
     1381 ctcgtctgcg ccggcgctgg cgccgcagcg ctcgcctgtc tcaacctgct cgtgaagatc
     1441 ggcatcaagc gcgagaatat ctacgtctgc gatatcgaag gccttgtcta cgaaggccgc
     1501 aagaagttga tggacgaata taaggcgccg ttcgcgcaga agacgaatgc acggacgttg
     1561 gctgacgtga ttggtggcgc cga
//
LOCUS       DASWOG010000316         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_190745,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000316 DASWOG010000000
VERSION     DASWOG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190745"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..2542
                     /locus_tag="VGB93_06525"
     CDS             371..2542
                     /locus_tag="VGB93_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY0145793.1"
                     /translation="MEDTLPNAQDEQFQFTIPALQERRTRTLKHGDTFAMFDDNGDVL
                     SSPGNPEGIFHRDTRHLSHLSLSIFGQRPILLSSTLRDDNGALICDLTNPDLHKGDHI
                     ALEHDLIHLRRLKFVWQNANFERLAVRNFSERRERVKIQIDFAADFADLFEVRGSKRE
                     RRGTYHPTSVKADSVVMSYTGLDDRRRDTQLRFSPQPTHLTANRAIFEVDLEPSERAS
                     IFIEIRCENVSPNIPPWRLFFSSLRDARRDLRAALAREASVETSNDIFNEVVRRSVAD
                     LHMLTTDLPEGPYPYAGIPWFSTVFGRDALITALQTLWIDPAIARGVLFYLAANQADK
                     IDAAADAEPGKILHESRRGEMAELGEVPFRRYYGSVDSTPLFVVLAGAYLQRTDDARA
                     LKRLWPHIEAALNWIDVYGDRDGDGFVEYGRKSDDGLLNQGWKDSHDSVFHADGTLAQ
                     GPIALVEVQGYVYAAKRAASTIALRLDNHDRALKLEYEAEQLQKHFDAEFWDEALGTY
                     VLALDGEKRPCRILTSNAGHTLFSGIALASRAPQIVRNLTDHDGFSGWGIRTVAASEA
                     RYNPMSYHNGSVWPHDNALIALGFARYGFGAEVTRIFKGLFEASIYIDLRRMPELFCG
                     FRRVRGQGPTFYPVACSPQAWAAATPLAVLSASLGLSFDPPNRHVIFNRPHLPDFLEE
                     VTLNGLTIGDASLSVQLRRTGTDVAVNVLSRKGDIWATTIS"
     gene            complement(2539..4338)
                     /locus_tag="VGB93_06530"
     CDS             complement(2539..4338)
                     /locus_tag="VGB93_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0145794.1"
                     /translation="MDDLCQYANRPTGFVLRYVKLQPISHLIISICVLAAVGCSVSTQ
                     FGIKFLVDSLSKGVGNANPWTAFALLCGFIAADNLLWRVAGWIAASSFVRVTGDMRRE
                     MFQHLMGHSPSYFSNRLPGTLTSRITATSNAAFTIQNMFIWNVLPPCVATVGAIAFIA
                     AVNLQMAAVLSVIAGIVVAVIFRVAAKGRPIHQDFAEKAAAVDGEMADVVGNVLLVKA
                     FGGFTSERNRFAGKVGQEMGARRRSLRYLEKLRLFHAVATVILVAGLLAWAILLWQHG
                     AASTGTVVLVCTLGVSVLAATRDLAVALVDITQHMARLAEALRTLLVPHELGDRPGAV
                     ALARAGAEITFQDVSFHYPGGPQVVKNFSLHIRPGEKVGLAGPSGGGKSTLLALMQRM
                     HDVQTGRILIDGQDIAMITQESLRAAIAVVPQDISLFHRSLIENIRYSRPDASDEDVM
                     KAAAAAHCDFIDDLPDGFNTMVGDRGLKLSGGQRQRIALARAFLKDSPILLLDEATSA
                     LDHESEEAIREASVRLMSGRTVIAIAHRLTTLRDFDRIVMLQDGELVQEGPPGELIDV
                     NGHYSDFARRKMTAQRKYHESADGSVRRYAMDA"
BASE COUNT          976 a         1410 c         1318 g          910 t
ORIGIN      
        1 cgagacggaa gaccattatt gcaaagctct cacgcccaag cccgcatttc aagaatcggc
       61 aatactgcaa gaacgaacat atcttgcacg agcccgttgg catctctgca agcgccaaaa
      121 aaaagttgca atccgcactg aacatcgtgc ttacggattg atatcgtcac acatgcgagc
      181 tatccgcaat gacgctgcat cgcccggcga gcctgtcaaa aacgcggcct cggaagcgac
      241 aatacttcag ttcaagcttc agacatggaa actccgggtg gcttccttcc ggacatgtca
      301 aaatcttggt tagctggacg tcttgagaca gagattccgc tgacaccttc aggaataagg
      361 ccgacgtatc atggaagaca ctctgccgaa tgctcaagac gagcaattcc agttcacaat
      421 tccggcgctt caggaacgcc gcacgcgcac attgaagcac ggcgatacgt tcgcaatgtt
      481 cgatgacaat ggcgatgtac tgtcctcgcc cggcaacccc gaagggatat ttcatcgcga
      541 cacgcggcat ctgtcgcatc tgagtttgtc gatcttcggc cagcggccga tcctgctgag
      601 ctcgacattg cgcgacgaca atggcgcgct gatctgcgac ctgacgaatc ccgatctgca
      661 caagggtgac catatcgccc tagagcatga tctcattcat ctgcggcggc tgaaattcgt
      721 ttggcagaac gcgaacttcg agcggctggc ggtgcgcaac ttcagtgagc ggcgcgagcg
      781 cgtcaaaatc cagatcgatt ttgccgccga tttcgcagac ctattcgagg tacgcggcag
      841 caagcgcgaa cggcgcggca cctatcatcc gacctcggtg aaagcggatt cggtcgtcat
      901 gtcctacacg ggcctcgatg accggcggcg ggatacgcag ctgcgtttct cgccgcagcc
      961 cacacatctg acagccaatc gcgcgatctt tgaagtcgat ctcgaaccaa gcgagcgcgc
     1021 gtcaattttc atcgaaattc gctgcgagaa tgtgtcgcca aatatcccgc cgtggaggct
     1081 gttcttttcc tcgctgcgcg atgcaaggcg cgatcttcgt gccgcgctgg cacgcgaggc
     1141 ttcggtcgag acctccaacg acattttcaa cgaagtcgtg cgccgctccg tcgccgacct
     1201 tcatatgctg acgacggacc tgcccgaggg cccctaccct tacgccggca tcccatggtt
     1261 tagcactgtc ttcggccgcg acgctttgat cacggctttg cagacgctct ggatcgaccc
     1321 ggcgatcgcg cgcggcgtcc tgttctatct ggcggcaaat caggccgata aaatcgacgc
     1381 agcggccgac gccgagcccg gcaagatttt gcacgagtca cggcgcggcg agatggccga
     1441 actcggcgag gtgcccttcc gccgttacta cggcagcgtc gattccacgc ctttgtttgt
     1501 cgttcttgcc ggtgcttatc tgcaaaggac ggatgatgcg cgcgcgttga agcggctctg
     1561 gccccatatc gaagcggcgc tgaattggat cgacgtttac ggcgaccgcg acggcgatgg
     1621 attcgtcgaa tatggccgca agagcgatga tgggctactc aatcagggtt ggaaagacag
     1681 ccacgattcc gtcttccatg ccgatggcac attggcgcaa ggtcccatcg cgctcgtcga
     1741 agtccaaggc tatgtctacg ctgcaaaacg cgccgcgtcg acaatcgcct tgcgcctcga
     1801 caatcatgat cgtgccttga agctcgagta tgaggccgaa cagcttcaga aacattttga
     1861 tgcggagttc tgggacgagg cgcttggtac ctacgtcctg gcgctcgatg gcgaaaaacg
     1921 gccctgccgc atccttacct caaatgctgg ccacactctg ttcagtggca tcgcactagc
     1981 atcgcgcgcg ccgcaaatcg ttcgcaatct cacagaccat gacggctttt ccggctgggg
     2041 catcagaacc gtcgcagcgt ccgaggcacg ctataacccg atgagctatc acaacggctc
     2101 cgtctggccg cacgacaatg cgctgatcgc attgggcttc gcgcgctatg gctttggcgc
     2161 ggaggtaaca cggattttca aaggcctgtt tgaggcgtcg atctatatcg atctgcgccg
     2221 catgccggag ttgttttgcg gcttccggcg cgtgcgtggc cagggaccga ccttttatcc
     2281 tgtcgcctgc tcgccgcagg cctgggcggc cgcgactcct ctggcggtac tctccgcgtc
     2341 gctcggcctc agcttcgatc cgcccaatcg tcatgtcatc ttcaaccggc cgcacctgcc
     2401 ggattttctt gaagaggtga cactgaacgg cttgacgatc ggcgacgcca gcctgtcggt
     2461 tcagctccgg cgcacgggga ccgacgttgc cgtcaatgtg ctgtcgcgca agggcgacat
     2521 ctgggcaaca acaatcagct aggcgtccat cgcgtagcgc cgaaccgatc cgtcggcgga
     2581 ctcgtgatat ttccgctgcg ccgtcatctt ccggcgcgcg aaatcgctgt aatggccatt
     2641 aacgtcgatc agctcgccgg gtggtccctc ctggacgagt tcgccgtcct gaagcatcac
     2701 gatccggtcg aaatctcgca gggtcgtcaa acggtgcgca atcgcgatga cggtgcgccc
     2761 actcatcaac cgcaccgagg cttcgcgaat ggcttcctcg gattcgtgat cgagagccga
     2821 ggtcgcctca tcgagcagca ggatcggtga atccttcagg aacgcccgcg ccagcgcgat
     2881 gcgctgacgc tgaccgccgg acaatttcag cccgcgatcg ccgaccatcg tgttgaagcc
     2941 gtcggggaga tcgtcgatga aatcgcaatg tgccgcggcg gcggccttca tcacgtcctc
     3001 gtcggaggcg tccggacggc tgtagcggat gttctcgatc aacgaacggt ggaaaaggct
     3061 gatgtcctgc gggacgacgg cgatggccgc gcgcagactt tcctgcgtga tcatcgcgat
     3121 atcctgacca tcgatcagga tgcgccccgt ctgcacgtcg tgcatacgct gcatcagagc
     3181 cagcaaagtc gatttgccgc cgcccgaagg tccagcaaga ccgaccttct cgcccgggcg
     3241 aatgtgcaga ctgaagtttt ttacaacttg cggcccgccg ggataatgga aggacacatc
     3301 ctggaaggtg atttcggccc cggcgcgggc gagcgcaacc gcgccggggc ggtcgccaag
     3361 ttcatgcggg accaataggg tccgcaaggc ttcggcgagc ctcgccatat gctgcgtgat
     3421 gtcgaccagc gctacggcca gatcgcgtgt ggcggcaaga accgagacgc ccagcgtgca
     3481 gacgagaacg actgtgccag tgcttgcggc gccatgctgc cagagcaaaa ttgcccaggc
     3541 cagaagaccc gcgaccagga tcaccgtcgc tacggcgtgg aacaggcgta atttttcaag
     3601 gtagcgcaga ctacgccgcc gcgcgcccat ctcctgcccg accttaccgg cgaagcggtt
     3661 gcgctcgctg gtgaagccgc cgaaagcttt gacgagcaaa acgttgccca cgacgtcggc
     3721 catctcgccg tcgacggcgg cggccttttc ggcgaaatcc tggtgaatcg gacggccttt
     3781 cgcggcaact ctgaaaatca ccgcaacgac aatgccggca atgacgctca ggaccgcagc
     3841 catttgcaga tttacagcgg caatgaacgc gatggcgccc acggtcgcaa cacacggcgg
     3901 cagcacgttc cagatgaaca tattctggat cgtgaacgcc gcattggacg tggccgtaat
     3961 gcggctcgtc aacgtgccgg gcaaacggtt ggaaaaataa ctcggcgaat ggcccatcaa
     4021 atgctggaac atttcgcgac gcatatcacc cgtcacgcga acgaatgaac tggctgcaat
     4081 ccaccccgcg acgcgccaca gcaaattatc agctgcgatg aagccgcaca agagtgcaaa
     4141 ggctgtccag ggattggcat tgcccacgcc tttcgacagg ctatcgacca gaaacttgat
     4201 gccaaattgc gtgctcaccg agcaacccac agccgccagc acgcagatcg atatgatcag
     4261 atgcgagatt ggctgaagct tgacatagcg aagaacaaaa cccgtcggcc ggttggcgta
     4321 ttggcaaaga tcgtccatcc aagaccttcc cccggtcggg attcgctaca aattgaatac
     4381 cggcgattat tggacagaga tctgcttgtt cgaccggcta atcacaaacg ccaagcgcag
     4441 ctttaagttc ctttcgaatg tgtgcagcgc cgaaaagttt gaacaaaacc gggctgtcgt
     4501 gcgttgtctt cgcagtctcg gggcgcgact gacggcgatt tacagatcgc tcattctgta
     4561 attgcctggc aatccgcgac ggcgctcgaa atgtcgttca cgaggattca ttcc
//
LOCUS       DASWOG010000317         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_227318,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000317 DASWOG010000000
VERSION     DASWOG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227318"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1230
                     /locus_tag="VGB93_06535"
     CDS             94..1230
                     /locus_tag="VGB93_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0145795.1"
                     /translation="MSLFLKRLGIWALAGLGFAATNLAATNNARAADLDYGKPGDPIH
                     LVVGYQPYYSEAWSGAVINGLQLWKKYLPAGSTVEFHVGLQGAIIVNAMLAGKANLGY
                     LGDMPAIVGATKRNVADLRILANIGLGHDECNVFFVRSDAPQFADAKTAVAWLNGKTV
                     ATPKGSCSDRFAQAVFQKNNVTPSAYLNQSIEVITSGFRVGKIDGAVLWEPTASRLVA
                     EGLARRVASGYNFNEPDGAFLDARADLIKQRPDVIKAWLETELDAELFLADPKNAGKV
                     AELLKAQTTGFTQKELWQAVFGAYPAASGGGPIRLTLPFGFSKDALALIAKDTAFLHS
                     VKSIAVEKLADDAILPNYTAEVLKERKLAVPVGVIKSAPETAFR"
     gene            1242..>1960
                     /locus_tag="VGB93_06540"
     CDS             1242..>1960
                     /locus_tag="VGB93_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0145796.1"
                     /translation="MSDLQELIAKALPFKLSPPVLAELAETAQPVEALEGDLIYESGA
                     TANALYIVRDGTILLEPSGGSAPLPSQAVKTGEAFGWTALGGKAGRRYRAIAVTAASL
                     LRIDGNRVLDLAAAESRDSTAMREQVAGALQDIRYSENRRSEAVELALYRFAHWLRSP
                     APYLGLIGYALLLGFWYMTVEVLKLPRFAEMPGLTQVLQEWLSRDPTYGVSIYTPVYY
                     QHILVSVQRIAIAFTLATTLGV"
BASE COUNT          379 a          630 c          567 g          384 t
ORIGIN      
        1 tcatcgacgt cggcgatcgc cgcctcgtca ttctccggca tgacgcgctt cgcgcgatcg
       61 ccggctgctg attaccgaac aggagaaaat tccgtgagcc tatttctaaa acgtctgggt
      121 atttgggcat tggctggtct cggctttgcc gcaaccaatt tggccgcaac caataacgcg
      181 cgcgccgcgg acttggatta cggcaagccc ggtgatccaa ttcatctcgt cgtcggctat
      241 caaccctatt acagcgaggc ttggtccggc gccgtcatca atggcctgca attgtggaag
      301 aaatatttgc cggccggctc gacggtcgaa tttcacgtcg gcttgcaggg cgcgatcatc
      361 gtcaacgcca tgctggcggg caaggcgaac ctcggctacc tcggcgatat gcccgcgatc
      421 gtcggtgcga cgaagcgcaa tgtcgccgat ctgcgcattc tcgccaacat tggtctcggc
      481 catgacgagt gcaatgtctt tttcgtgcgc agcgatgcgc cacaatttgc cgatgccaaa
      541 accgccgtcg cctggctcaa cggcaagacc gtcgccacgc cgaagggaag ctgctcggat
      601 cgcttcgcgc aagcggtctt ccaaaagaac aacgtgacgc cgagcgctta tctcaatcag
      661 agcatcgagg tgatcaccag cggcttccgt gtcggcaaga tcgacggcgc cgtactctgg
      721 gagccgacag cctcgcgcct cgtcgccgaa ggcctcgccc gccgcgtcgc cagcggttac
      781 aatttcaatg agccagacgg cgcgtttctc gatgcccgtg cagatctcat caaacagcgg
      841 ccggacgtca tcaaggcctg gctcgagacc gagcttgatg ccgaactctt cctcgccgat
      901 ccaaagaacg ccggcaaagt cgctgaactc ctgaaagcgc agaccaccgg cttcacgcaa
      961 aaggaattgt ggcaagcggt attcggcgcc tatcccgccg caagcggcgg tggtccgatc
     1021 cggctgacct tgcccttcgg attctcaaaa gacgcgcttg cgctcatcgc caaggacacg
     1081 gccttcctgc attcggtcaa gagcattgcc gtggagaagc tggcagacga cgccatcctg
     1141 ccgaactata ccgccgaagt tctgaaagag cgcaagcttg cggtgccggt cggcgtgatc
     1201 aaaagcgcgc ccgagaccgc cttcagatag ggccgccgcg gatgagcgac cttcaagaac
     1261 tcatcgccaa ggctttgccg tttaagctgt cgccgcccgt tctcgcggag cttgcggaaa
     1321 cggcgcagcc ggtcgaagcg ctggaaggcg atctcatcta cgagtcaggc gcaaccgcga
     1381 atgcgctcta catcgttcgc gacggtacca tcctgctcga accttctggc ggcagcgcac
     1441 cgctgccgtc acaagccgtg aaaacaggcg aggccttcgg ctggacggcg ctcggcggca
     1501 aggccggccg ccgctatcgc gcaatcgcag ttaccgccgc gagcctcctc cgcattgacg
     1561 gcaaccgcgt ccttgacctt gctgccgccg aatcgcgcga cagcaccgcg atgcgcgaac
     1621 aagttgcagg cgcgttgcag gatatccgtt actccgaaaa tcgccgttcg gaagccgtcg
     1681 aacttgcgct ttatcgtttc gcgcactggc tgcgctcgcc ggcgccttat ctcggcttga
     1741 tcggttacgc cctgctgctg ggcttctggt acatgacggt cgaagtattg aagttgccgc
     1801 gctttgctga aatgccaggc ctgacgcagg ttctgcagga atggctaagc cgcgacccga
     1861 cctatggcgt ctcgatttat acgcccgtct attatcagca cattcttgtc agcgtgcagc
     1921 ggatcgcgat cgccttcacg ctggcgacga cgctcggcgt
//
LOCUS       DASWOG010000318         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_264052,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000318 DASWOG010000000
VERSION     DASWOG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264052"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..184)
                     /locus_tag="VGB93_06545"
     CDS             complement(29..184)
                     /locus_tag="VGB93_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY0145797.1"
                     /translation="MLSTILIIILILALIGGLPNWGYSSGWGYYPSGGVGLILLIVII
                     LVLLGRI"
     gene            complement(300..611)
                     /locus_tag="VGB93_06550"
     CDS             complement(300..611)
                     /locus_tag="VGB93_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145798.1"
                     /translation="MSPVETGQWQPMATAPKDGTRIIVVTRSTEQGTSDVDVVRWMKP
                     KSFGEFCWVATDSDMDCIVMYDEWEVANWMPLPSWSPPYKTPELVAKLPEPPEAEGSG
                     I"
     gene            complement(701..2521)
                     /gene="aspS"
                     /locus_tag="VGB93_06555"
     CDS             complement(701..2521)
                     /gene="aspS"
                     /locus_tag="VGB93_06555"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0145799.1"
                     /translation="MHLYRTHTCGDLREAQAGAEVRLSGWCHRIRDHGGVLFIDLRDH
                     YGITQCVVDPDSPAFPQAEKLRSEWVVRIDGTVRQRPAGTENSEMPTGFIEVYVNAIE
                     VLGPAAELPLPVFGDQQYPEEMRLKYRFLDLRRDKLHKNIMLRGAIIDSLRARMKAQG
                     FFEFQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDCEMSFVTQEDVFAAIEPVLRGIFEEFGDGKPVTQKFPL
                     IPYAEAMLKYGSDKPDLRNPLVIVDVTAEFARDDVTFNAFKNVIKAGGVVRAIPAPGA
                     GAQPRSFFDKLNDWARGEGAPGLGYVIFENGEGGLAGKGPIAKFLPPEVQQAIAAKAG
                     IGAGDAVFFVCDQEAKAAKLAGAARLKIGSELGLSRTGVFEFCWIVDFPMYEWNEEDG
                     KIDFSHNPFSMPNFAHDDFVALDAADKETILAIKAFQYDIVCNGIELSSGAIRNHRPD
                     IMRKAFALAGYGEDVLEQKFGGMYRAFQYGAPPHGGIAPGVDRIVMLLAGEENLREVV
                     VFPMNQRAEDLLMSAPSPVTPKQLRELHIRLNLPEVKGTN"
     gene            complement(2559..3110)
                     /locus_tag="VGB93_06560"
     CDS             complement(2559..3110)
                     /locus_tag="VGB93_06560"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0145800.1"
                     /translation="MSKRSFFAILLLSAGLAGPALAEDSTAKPDESHPAHASGTTTKA
                     FGPWSLHCQPPHQEGAEPLCVIAQTVESSSNKLLAVISIGHQHPSDPYNIVVTLPPNV
                     AFPSSVHIRTGEQDKWGVELPWQRCIPGACIAGTELSPATLVHWAGLQTEGKIVFVVG
                     SGDEVAVPISLRGFGDAYKALNQ"
     gene            3222..4400
                     /gene="rnd"
                     /locus_tag="VGB93_06565"
     CDS             3222..4400
                     /gene="rnd"
                     /locus_tag="VGB93_06565"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY0145801.1"
                     /translation="MSLITSNHELAEACRRFADHPFITVDTEFLRETTFWPQLCVVQI
                     ASPDEAIAVDALSEGIDLSPVFALMANKAVVKVFHAARQDIEIVWNLARLIPTPLFDT
                     QVAAMVCGFGEQIAYGELVQSVCKVTLDKSSRFTDWSRRPLSEAQANYAIADVTYLRD
                     IYLYLRKKLESTGRLAWLDDEMAALTSADVYEQHPERAWERFRGRARRPRDLAVLMEL
                     AAWREAEAQARDVPRGRVLKDDVLVEIASAAPKTADELGNLRAFPRGMERSRAGADIL
                     AAVQRGLARDPKTLPKIERERRNGNGATVELLKVLLRQVSEAHGVAAKMIATVEDLEV
                     LATNKSAKIPTLSGWRREIFGEKALELIEGRLALTLEKGKVIALEWHDAPEAKESQAV
                     "
     gene            complement(4397..5056)
                     /locus_tag="VGB93_06570"
     CDS             complement(4397..5056)
                     /locus_tag="VGB93_06570"
                     /inference="COORDINATES: protein motif:HMM:NF044545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145802.1"
                     /translation="MREGLLYEQLSSAEQRLDPLIFAAQTFNDAYARSPRHAEELRQW
                     TDHFMGSLRFDETPEDKRLRHAACLLADVNWRAHPDYRSEQSLNIVVNGAFTGIDHPG
                     RCFLALVMSYRYLGLDADVNPQVRAHVSPRLLDQARLLAGVMRVAYAVSAGMADVLPR
                     APLIASKGKTILTLPADLADLAGERLSNRQKQLAKLIGREAVLQTGVAVKTNGSSVET
                     A"
BASE COUNT         1048 a         1588 c         1558 g          965 t
ORIGIN      
        1 aagctgctgc ttcgcagcat tgcagaggct agatgcggcc cagcagcacg agaatgatga
       61 cgatcagcag gatgagacca acgccgccgg aaggataata gccccagccc gaggaataac
      121 cccagttcgg caggccgccg atcaaggcca agatcagaat gatgatcaat attgtcgaga
      181 gcattggtat cctccacctg agctttgcgt gatgctcaga cgaggctaac ggcgaacctc
      241 actccgaagt tccggcttcg gtgacttgtc agggcgtcga ttggtgcgcg ccgcacgcgt
      301 cagatgccgg atccctcggc ttcgggcggt tccggaagct tcgcaacaag ctccggcgtc
      361 ttgtacggcg ggctccagga cggcaagggc atccagttag cgacttccca ctcgtcgtac
      421 atgacgatgc aatccatgtc cgagtccgtc gccacccagc aaaattcgcc gaacgacttc
      481 ggcttcatcc agcgcacgac atcgacatcg gacgtgccct gctccgtcga ccgcgtgacg
      541 acgatgatcc gcgttccatc ctttggcgct gtcgccatcg gctgccactg accagtttca
      601 acaggagaca tgagatactc cgcaactgga tatctgaacg tacgacgtca aaccggcaga
      661 acttcccacg ccgcggtaag tccggagcgg cgagagacct tcagttcgtt cccttcacct
      721 caggcaaatt cagcctgatg tgcaattcgc gcaattgctt cggcgtcacc ggcgacggcg
      781 cgctcatgag cagatcttcg gcgcgctggt tcatcgggaa gaccacgact tcacgcagat
      841 tttcttcacc cgcaagcagc atgacgatgc gatcgacgcc cggcgcgatg ccgccgtgcg
      901 gcggggcgcc atattggaag gcgcgataca tgccaccgaa cttctgctcc agcacgtcct
      961 caccatatcc ggcgagtgcg aacgccttgc gcatgatgtc gggccgatga ttacgaatgg
     1021 cgcccgacga caattcgatg ccgttgcaaa cgatgtcgta ttgaaacgcc ttgatcgcaa
     1081 gaatcgtttc cttgtcggcc gcatcgagcg caacgaaatc gtcgtgcgcg aaattcggca
     1141 tcgaaaacgg attgtgcgag aagtcgatct tgccgtcttc ctcgttccat tcgtacatcg
     1201 ggaaatcgac gatccagcag aactcgaaca cgcctgtcct cgacagtcca agttcgctgc
     1261 cgattttgag ccgcgcagcg ccggcaagct tcgccgcctt cgcttcctga tcacaaacga
     1321 aaaagacagc atcgcccgca ccgataccgg ccttcgcggc gatcgcctgc tgaacctcag
     1381 gcggcagaaa cttcgcgatg gggcccttgc cagcaaggcc gccctcacca ttctcgaaaa
     1441 tcacataacc cagccctggc gcgccttcgc cgcgggccca atcgttgagc ttgtcgaaga
     1501 agcttcgcgg ctgcgcgccc gcgcccggcg ccggaatggc ccgcacgacg ccgccagcct
     1561 tgatgacatt cttgaaggca ttgaaggtca cgtcgtcgcg tgcgaattcc gcggttacgt
     1621 caacaatgac gagtggattg cgcaaatccg gcttgtccga gccatatttc agcatggctt
     1681 cggcataggg gatcagcggg aatttctgcg tgaccggctt gccgtcgccg aactcctcga
     1741 agatgccgcg cagcaccggc tcgatcgccg cgaaaacgtc ttcctgcgtg acgaagctca
     1801 tttcgcaatc gagctgatag aattcgccgg ggctgcggtc agcacgcgca tcctcgtcgc
     1861 ggaaacacgg cgcgatctga aaatagcgat cgaagcccgc gaccatgaga agctgcttga
     1921 actgctgcgg cgcctgcggc agcgcataga acttgccggg atgcaggcgc gacgggacga
     1981 ggaaatcgcg cgcgccttcc ggactggaag cggtgaggat cggcgtctgg aattcgaaga
     2041 agccctgcgc cttcatccgc gcacgcaggc tgtcgatgat cgcgccgcgc agcatgatgt
     2101 tcttgtgcag cttgtcgcgg cgcaaatcga ggaagcgata tttgagccgc atctcctcgg
     2161 gatattgctg atcgccgaag acaggcagtg gcaattcggc agcaggcccc aagacctcga
     2221 tggcgttgac atagacttcg atgaagcccg tcggcatttc cgaattttcg gtgccggccg
     2281 ggcgttggcg caccgtgcca tcaatgcgca cgacccattc cgaacgtagc ttttccgcct
     2341 gtggaaaggc cggcgaatcc gggtccacga cacattgggt gatgccgtaa tggtcgcgca
     2401 gatcgatgaa cagcacaccg ccatgatcgc ggatgcgatg gcaccagccg gaaagacgga
     2461 cttcagcgcc ggcctgagcc tcgcgcaggt cgccacacgt atgagtacga taaagatgca
     2521 tgtcccgtca catcagatgc gcgcctctcg gcgcgggttt attggttgag cgctttgtag
     2581 gcgtcgccaa agccacgcag gctgatcggc acggcgacct cgtcgccgga tccgacgaca
     2641 aacacgatct tgccttcagt ctggagaccg gcccaatgca caagggtcgc cgggctcagc
     2701 tcggttccag caatgcaggc acctgggatg cagcgctgcc acggaagctc cacgccccat
     2761 ttgtcctgct caccggtacg gatatgcacc gaactcggaa acgcgacgtt gggcggcaag
     2821 gtgacgacaa tgttataagg atcgctcggg tgctggtggc cgatcgagat gacggcaaga
     2881 agcttgttac ttgagctttc caccgtttgg gcgatcacgc agagcggctc cgcgccttcc
     2941 tgatgcggag gctggcagtg taggctccag ggcccgaagg ccttcgtggt cgtgcccgag
     3001 gcgtgagcag gatgcgattc atcgggcttc gccgtcgaat cctcggccag agccggcccg
     3061 gcaaggcctg cggagagaag taaaatcgcg aaaaaggacc gtttcgacat ggcttcccgc
     3121 tgctccgacg ttgggaggcg ccctccatat acgagaaact ggcagatcga taggcccacc
     3181 caacactggc agcgcggcca cctttccagt atcaagcttt gatgagcttg attacctcga
     3241 atcatgaact ggcggaggcc tgccgccggt tcgctgacca cccctttatt accgtcgata
     3301 cggaatttct gcgggaaacc accttctggc cccaactctg cgtcgtgcag atcgcttcgc
     3361 cggacgaggc gatcgccgtc gatgcgcttt cggaaggcat tgatttatcg ccagtttttg
     3421 ctttgatggc gaataaagcc gtggtcaagg tgttccatgc cgcccgccaa gatatcgaaa
     3481 tcgtctggaa tctcgcccgg ctgatcccga cgcccctgtt cgacacccag gtcgcggcca
     3541 tggtctgcgg cttcggcgag cagatcgcct atggcgagtt ggtccagtcg gtctgcaagg
     3601 tcactctcga taaatcgtcg cgctttaccg attggtcgcg ccgcccgctc tcggaggcgc
     3661 aggccaatta cgccatcgcc gacgtgacct atctgcgcga catctatctt tatttacgca
     3721 aaaagctgga atcgaccggc cgactcgcct ggctcgacga tgagatggcg gcgctgacct
     3781 cggccgatgt ctatgagcag catcccgaac gagcctggga acggtttcgc ggccgggcac
     3841 ggcggccccg cgatctcgcc gtcctgatgg agcttgccgc ctggcgcgag gccgaggcac
     3901 aggcccgcga cgtgccgcgc ggccgtgtgc tcaaagatga tgttctcgtc gagatcgcca
     3961 gcgccgcacc gaaaacggcg gacgagcttg gtaatctgcg ggccttcccg cgcggcatgg
     4021 aacgctcgcg cgccggcgcc gatattctcg ccgccgttca gcgcggcctc gcgcgcgatc
     4081 caaaaacttt gccgaagatc gagcgtgaac ggcgcaatgg caatggtgcg actgtcgagc
     4141 ttctaaaggt tctgctgcgg caagtcagcg aagcccatgg cgtcgcggca aagatgatcg
     4201 cgacggtcga ggatttagaa gttctcgcga ccaacaaaag cgccaaaatt cccacgctca
     4261 gcggctggcg gcgtgagatt ttcggcgaaa aagcgctgga gctgatcgag gggcgcctcg
     4321 cactcaccct ggaaaaaggc aaagtgatcg cgctcgaatg gcatgacgca ccggaagcta
     4381 aagaaagcca ggcggtctaa gccgtttcga ccgagcttcc gttcgtcttc actgcaactc
     4441 cggtctggag cacagcctcc cggccaatca gcttggcaag ctgcttctgg cggttgctta
     4501 gccgttcgcc ggccaggtcc gcgagatcag ccggcagcgt gagaattgtc ttgcctttgg
     4561 aggcaatcag cggcgcccgc ggcagcacat ccgccatacc ggccgagacg gcgtaagcga
     4621 cacgcatgac gccggcgagc aagcgcgcct gatcgagaag ccgcggcgaa acatgcgcgc
     4681 ggacttgcgg gttcacatct gcgtcgaggc cgagatagcg ataggacatg acgagcgcga
     4741 ggaagcagcg ccccggatga tcgatgccgg tgaaggcgcc attgacgacg atgttgagac
     4801 tttgctcgct acggtaatcg ggatgcgcgc gccagttgac gtcggcgagc aggcaggctg
     4861 cgtggcgaag ccttttgtcc tcgggcgttt cgtcaaaccg cagcgagccc ataaagtggt
     4921 cggtccattg ccgcagttcc tcggcgtgac ggggcgagcg cgcataggca tcgttgaacg
     4981 tctgcgcggc aaaaatcagc ggatcgaggc gttgctcggc gctcgacaat tgttcgtaca
     5041 gcaaaccttc gcgcacgccc gaggcggaaa tggttacctg ttttggctgc gccttgcgaa
     5101 tgatctcgtc gagaacgatc gcgccctagg cgagcagggg acgccgcgcc ggcgtcacc
//
LOCUS       DASWOG010000319         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_190801,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000319 DASWOG010000000
VERSION     DASWOG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190801"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..559
                     /locus_tag="VGB93_06575"
                     /pseudo
     CDS             234..559
                     /locus_tag="VGB93_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            773..2389
                     /locus_tag="VGB93_06580"
     CDS             773..2389
                     /locus_tag="VGB93_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0145803.1"
                     /translation="MRPGFLSKLKVTVGDRNVPALQLWIACNFVSMALILVLGMRLGW
                     AFFASHASLSSLSLDFAWMALVSNLWLLAAIITVRLVMAWLAYHVLDGLRSSEAAARQ
                     VAGRDALSGLPNRFLFNALVDAEIARCKRGRHQFALFYLDLDHFKQANDTFGHDVGDR
                     LIVAVTRRLADVLRSCDHFARLGGDEFALMQADVNEPRDCARLAQRILDAMSAPFDIG
                     GQQIFIGISIGIALCPQNATDCQELMRLADLALYRSKQAGRNRFAFFEAKMGEELRMR
                     QSAEDELRTAIENNELELLYQPIISSTDDKIICVEALARWRHKTYGLLSADSFIPLAE
                     ERGLIVQLGEWALRRACADAMRWPGIKVAINVSPIQFRQKDFVATVNRILTESGIDPQ
                     RVELELTEGVIIADADLAEHSMINLRALGVHMVLDDFGTGYSSLIYLRRFAFDKIKID
                     RSFLESMEDSGESVIIVRSIVDLGRSLGLRVTAEGVETEEQARLLRSIDCDELQGFLF
                     ARPMSVEDIDQRLEDENARELLPVSARSVA"
     gene            complement(2392..2691)
                     /locus_tag="VGB93_06585"
     CDS             complement(2392..2691)
                     /locus_tag="VGB93_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEY0145804.1"
                     /translation="MANGSVSGRAPVSDDFIRQLAGYGLTTAEILYRMPDHPLVLQSF
                     LWQDYDLAPKFPVLTKFLAFWQEKLEGPLYSVRVGHSALIKPAEIRTFETEFRIH"
     gene            complement(2875..>3089)
                     /locus_tag="VGB93_06590"
     CDS             complement(2875..>3089)
                     /locus_tag="VGB93_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145805.1"
                     /translation="GGGWHGGGWHGGGWHGGGGGWHGGGGGWHGGGGGWHGGGHGGGF
                     HGGGGFHGGGGHGGGGHGGGGHGGHR"
BASE COUNT          585 a          967 c          867 g          670 t
ORIGIN      
        1 atcgtctgac ctgccccaca ccggcaagcc gatcggcccc gttggcgact cccgcctgcg
       61 cggcgatagc taacgtccag cggcaagtcc ctatcgggaa gccccaatag tattcgcggt
      121 gggttaactt agtgttggga aaactgcccc ttggcgcaaa tagaatatca gccaaggcaa
      181 ttgcgccggc gatgggcagt ttcagcgccg tgctcgatta ttgccgcacg gcgatggcct
      241 tcctcgatcg cgaggaattc cgcattctat ttctcgacaa gaaaaacttt ttgatcgccg
      301 acgaggtgca atcgacaggc acgatcgatc acgcgccagt ctttccctgg gagatcatcc
      361 gccgcgcctt cgagctcaat gcgacggcgc tcatcctgtt cataaccatc cgtcgggaga
      421 cccgtcgccg tcacagaccg atatccagat gacccggcag atcgtttccc tcggcaaatc
      481 actgaatctc gccgtccacg atcatttgat catcggccgg gacggcttca cgagcttcaa
      541 gagccttcag cttatttaac ttaaatgcca tgctggcacg attgccttgg ccgatattct
      601 atttgctcca aggggcagtt ttcccaacac taagttaacc ccccgcgaat accattgggg
      661 cttctcgata gggacttgcc gctggacgtt agctatcgcc gcgcaggcgg gagccgccga
      721 cgaggccgac cggtttgccg gtgcggggca ggtcagacga tttggagtcg caatgcgtcc
      781 cggctttctg tccaagttga aggtgacggt cggcgatcgc aatgtgccgg cgcttcaact
      841 ttggatcgcc tgcaactttg tgtcgatggc gctcatcctc gtcctcggca tgcgcctcgg
      901 ctgggcgttc ttcgcgagtc atgcctcttt gtcatcttta tccttggact tcgcttggat
      961 ggcgctcgtc tcgaacctct ggctgctggc cgccatcatc acggttcggc tggtgatggc
     1021 ctggctggcc tatcacgttc ttgacgggct ccggagcagc gaagccgccg cgcggcaggt
     1081 ggccggtcgt gatgcactgt ccggtctgcc caatcgtttt ctcttcaatg ccttggtcga
     1141 tgcggaaatc gcccgctgca agcgcgggcg tcaccagttc gcgctgtttt atctggacct
     1201 cgatcatttc aagcaagcca acgacacttt cggccatgat gtcggcgacc gtctgattgt
     1261 ggctgtcacc cgacgcctgg ccgacgtcct gcgctcctgc gatcattttg ctcgtctcgg
     1321 cggcgatgag ttcgccctca tgcaggccga tgtaaacgag ccgcgcgatt gcgcccgtct
     1381 cgcacagcgt attctcgacg ccatgagcgc gccgttcgat attggcggcc agcagatttt
     1441 catcggcatc tctatcggta tcgcgctctg tccccaaaat gcgaccgatt gtcaggagct
     1501 catgcggctc gccgatcttg cgctgtatcg ctcaaaacag gccgggcgca accgcttcgc
     1561 ctttttcgag gcgaagatgg gcgaagagtt gcgcatgcgg cagagcgcag aagatgaatt
     1621 gcgcacggcg atcgagaaca atgaactcga acttctctat caaccaatta tctcgtcgac
     1681 cgacgacaaa atcatttgcg tcgaagcgtt ggcgcgttgg cgccacaaaa cttacgggct
     1741 gctgtcggcg gatagtttca tcccgctcgc cgaggaacgc ggccttatcg tccagctcgg
     1801 cgaatgggcg ctgcgccgcg cttgcgcgga tgccatgcgc tggcccggca tcaaggtcgc
     1861 catcaacgtg tcgccgattc aatttcggca aaaggatttt gtcgctaccg tcaatcgcat
     1921 tctgaccgag tccggaattg accctcagcg tgtcgagctt gaattgaccg aaggcgtcat
     1981 cattgccgat gcagacctcg ccgaacattc catgatcaat ctgcgtgcgc tcggcgtcca
     2041 catggtgctc gatgactttg gcactgggta ttcgagcttg atttatttgc gccggtttgc
     2101 tttcgacaaa atcaagatcg accgctcgtt tcttgaatcc atggaagact ctggcgaaag
     2161 tgtcatcatc gtccgttcca tcgtcgatct cgggcgttcg ctcggtctgc gcgttaccgc
     2221 ggaaggggtt gaaaccgagg agcaggcgcg actcttacgg tcgatcgact gcgacgaatt
     2281 gcagggcttt ttgttcgcgc gcccgatgtc ggtggaagac atcgaccagc gccttgagga
     2341 cgagaacgcg cgcgagcttt tgccggtctc cgcgcgctcg gtggcttaag ctcagtgaat
     2401 ccggaattcg gtctcgaacg tgcgaatctc ggccggcttg atcaaagccg agtggccgac
     2461 gcggacgctg tagagcggtc cttcgagctt ctcctgccag aacgcgagaa atttcgtcaa
     2521 aaccggaaat ttgggcgcga gatcgtagtc ctgccagaga aagctttgca ggacgagcgg
     2581 atgatccggc atacggtaga gaatctccgc cgtcgttaaa ccgtagcccg ctaattgcct
     2641 gatgaaatcg tctgaaaccg gcgcccgtcc gctcacgctc ccatttgcca tatcgcgatc
     2701 cccctctgga ggcctcatcc cggcgaatcg actgtcatcg ctccgccgta atggttaatt
     2761 tcgcgcgcaa atggttaacg aatttttgat gtaacagctt gagaagccgg ccgcgcggcc
     2821 aaaagaaatc ggcggctaat gccgccgatc gccaattcga aaaattggga agggttagcg
     2881 atggccgccg tggccaccac cgccgtgccc gccaccacca tgtccgccgc cgccatggaa
     2941 gccgccaccg ccgtggaagc cgccgccgtg gccgccgcca tgccagccgc cgccaccgcc
     3001 atgccaacca ccaccgccgc cgtgccagcc accgccaccg ccgtgccagc caccaccgtg
     3061 ccaaccaccg ccgtgccagc cgccgccac
//
LOCUS       DASWOG010000320         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_282009,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000320 DASWOG010000000
VERSION     DASWOG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282009"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VGB93_06595"
     CDS             complement(<1..663)
                     /locus_tag="VGB93_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0145806.1"
                     /translation="MSDLRLLDRAEQRVSASTEGGALALAAKLPSLIVAAKEVAASVT
                     HGMHGRRRAGIGETFWQFRPFVAGESTTGIDWRRSARDDRIYIREREWEAAHNVAIWI
                     DRSPSMGFISNLARQSKSDRALVLGLAAANLLVRGGERVSLIGLTRPLASRSIVAQFA
                     EAMIAEERTAGYQAAELAPNLVLPPRSHALLISDFLSDPAQIAARIKALAARGAHGYL
                     VMI"
     gene            complement(666..1667)
                     /locus_tag="VGB93_06600"
     CDS             complement(666..1667)
                     /locus_tag="VGB93_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0145807.1"
                     /translation="MAEANTTSLEAAVSRAAETALDRIAAARAALGSVIFGQHQVIEE
                     ALVTLLAGGHGLLVGVPGLAKTNLVVSLGKVLGLDARRVQFTPDLMPADILGSEVLEE
                     SADRRRSFRFVPGPIFAQLLMADEINRASPRTQSALLQAMQEHHVSVAGERHDLPNPF
                     HVLATQNPLEQEGTYPLPEAQLDRFLMQIDVPYPDRAAEKQILIETTGDKDQEARQAM
                     TTDDLLTTQRLVRRLPVGEQVVEAILDLVRSARPGEGDKMLTQHIAWGPGPRAAQSLM
                     LASRARALVDGRLSPSLNDIVALAEPVLKHRMALTFAARAEGETIPGIIAKLTQKLT"
     gene            1884..2351
                     /locus_tag="VGB93_06605"
     CDS             1884..2351
                     /locus_tag="VGB93_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0145808.1"
                     /translation="MSYLAHILQPDEKVLFDGRLHWIIYSRAILFAVLAGLCAGALYF
                     LGDQQGLKFSALSLAFIFAVIAIALALHSWWRAFMTEIAVTSTRVIYKTGFIRRFTVE
                     MNMDKVESVDVIQPLLGRLLDYGSVRVRGTGASLEQLNYVAHPLALRSAITAR"
     gene            complement(2360..2857)
                     /locus_tag="VGB93_06610"
     CDS             complement(2360..2857)
                     /locus_tag="VGB93_06610"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY0145809.1"
                     /translation="MEIREPRSKAGSIAGWPTGYSPVKSLQVLAEDISNISASNFERN
                     NKLLHDLQGARTVEDLISIQTKFMASMFEAFNDHIRLMGARMAEMRSDIVDAGGNGAA
                     TPPADITAKTASGLNHALDAANNAAMANFQAGQDITRSAFEAAEKAADAIRNAALHAI
                     PKSGE"
     gene            complement(2962..3123)
                     /locus_tag="VGB93_06615"
     CDS             complement(2962..3123)
                     /locus_tag="VGB93_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY0145810.1"
                     /translation="MLYWALIFLVIAIVAGVLGFGGVAGTASEVAHVLFWIAIILLIV
                     SLIFGLRRR"
     gene            complement(3311..>5257)
                     /gene="alaS"
                     /locus_tag="VGB93_06620"
     CDS             complement(3311..>5257)
                     /gene="alaS"
                     /locus_tag="VGB93_06620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0145811.1"
                     /translation="YDIDLMRALVLAVAQATGVDPDGPQKASHRVIADHLRASAFLVA
                     DGVLPSNEGRGYVVRRIMRRAMRHAQLLGAREPVMWRLVPVLVREMGQAYPELVRAEA
                     LIVETLRLEETRFINTLARGLTILDEETRDLPEGGVLPGAAAFKLYDTYGFPLDLTQD
                     ALRGRGLGVDVEGFNTAMQQQRADARKAWAGSGEAATESIWFGLKERVGATDFLGYET
                     ESAEGVIVALLKDGREVGRLAAGETGQVILNQTPFYGESGGQVGDTGTIRASNLSFRV
                     TNTHKKLGDLFVHDGTVESGHAAVDLSVELDVDHSRRDAVRANHSATHLLHESLRQVL
                     GDHVAQKGSLVAPDRLRFDFAHPKPISAEELAEVEDIANRVVLQNAPVETRLMDVDTA
                     IESGARALFGEKYGDQVRVVSMGDPLETAGGARANAAFSIELCGGTHVRRTGDIGLIS
                     IIAESPVAAGVRRIEAKTGLAARRHLNSQAQRFQDMAHLLKAPEDEAGARLAHVLDER
                     RKLERELSDARKRLAMSGGAADAPIEEVGGIKFFRKEVSGVEMKDLKSLADEAKQSLG
                     SGVVAIVGIDAEGKAGVVVGVTDDLTSRFDAVTLVRAAAAQLGGKGGGGRRDMAQAGG
                     PDGGSAQAALEAIAAALQQTADPA"
BASE COUNT         1022 a         1690 c         1566 g          979 t
ORIGIN      
        1 tatcatcacc agataaccat gtgcaccacg cgcggcgagc gccttgatgc gggcggcgat
       61 ctgcgccgga tcgctcagaa aatccgaaat gagcagcgcg tgtgagcgcg gcggcagcac
      121 aagattgggc gccagctccg cggcctgata ccccgctgtg cgctcctccg cgatcatggc
      181 ctcggcgaat tgcgcgacaa tgctgcgcga cgccagcggt cgtgtcaggc cgataaggct
      241 cacccgttcg ccgccgcgca ccagcagatt ggctgcggca agaccgagaa caagcgcgcg
      301 atcgctcttc gattgacgcg cgagattgga gatgaaaccc attgatggcg aacgatcgat
      361 ccagatcgca acgttgtgcg ccgcttccca ttcgcgttcg cggatataga tccgatcatc
      421 gcgggccgaa cggcgccaat cgatgcctgt cgtcgactcg cccgcaacaa agggacgaaa
      481 ttgccagaac gtttcgccga taccggcgcg tcggcgcccg tgcatcccat gcgtgacgct
      541 cgcggcaact tccttcgcgg caacaatcag actcggaagc ttcgcggcta gcgcgagagc
      601 accaccttcg gtggaagcgg aaacccgctg ctcggcgcga tcaagcaggc gcagatcgct
      661 catcctcagg tcaacttctg agtcagtttc gcgatgatgc cggggatcgt ctcgccctcg
      721 gcacgcgcag caaaggtgag cgccatgcga tgcttcagga cgggctcggc caaggccacg
      781 atatcgttca gcgatggcga aaggcggcca tcgacaagcg cgcgcgcgcg gctcgcaagc
      841 atcagcgatt gcgcggcgcg cggccccgga ccccaggcga tatgttgcgt gagcatctta
      901 tcgccttcgc ccgggcgggc ggagcgaacg aggtcgagga tcgcttcaac gacctgctcg
      961 ccgacgggca gacgccgcac gagacgttgc gtcgtgagga gatcgtccgt agtcatcgcc
     1021 tgccgcgctt cctgatcctt gtcgcccgtc gtctcaatga gaatctgctt ttccgcagcg
     1081 cgatcgggat aaggcacatc gatctgcatc aggaagcgat cgagctgcgc ctcgggcaaa
     1141 ggataagtac cctcctgctc aagcggattt tgcgtcgcga gaacgtgaaa cggattgggc
     1201 agatcgtgcc gctcgccggc gaccgagacg tgatgttcct gcatcgcctg aagcagcgcc
     1261 gattgcgtgc gcgggctggc gcgattgatc tcatcggcca tcagcaattg cgcgaagatc
     1321 ggccccggca cgaaacgaaa gctgcggcgc ctgtcggcac tctcttcgag cacttcggag
     1381 ccgagaatat cggccggcat caaatccggc gtgaattgta cgcggcgcgc atcaaggcca
     1441 agcaccttgc cgaggcttac aacgagattg gtctttgcga gccctggaac gccaacgagc
     1501 aaaccatgcc cccccgcgag aagcgtcaca agcgcttctt cgatcacctg atgctgaccg
     1561 aagatcacac tgcccaatgc ggcgcgcgcc gcagcgatcc ggtcgagcgc agtttccgcc
     1621 gcccgcgaca cggcggcttc gagagatgtc gtattcgctt cggccattcg cgatctcacc
     1681 tcggacgtga aaagacaatt gtggaccttt tagccggcaa agaccaccga tctaacggcg
     1741 gccgatcacc tgacattcgg aatggcaaag attaaagaaa tttgtggcag gacaatgcct
     1801 tattgtcgtg atcttatccc gcagttgagc gatgatcaat tgcggagctc ggctcctaac
     1861 ataacgtgga gttaatgtag cgcatgagct atctcgcgca cattcttcag cccgacgaga
     1921 aagttctgtt cgacgggcgt ctgcactgga tcatctacag ccgggccatt ctctttgcgg
     1981 tactcgcggg cctttgcgca ggtgcgctct attttctggg cgatcagcaa gggctgaaat
     2041 tttcagcgct gagcctcgca tttatttttg ctgtgatcgc catcgccctt gcactccata
     2101 gctggtggcg tgcgttcatg acggaaatcg cggtgacctc aacccgtgtg atttacaaga
     2161 ccggcttcat ccggcgcttc accgtcgaaa tgaacatgga caaggtcgaa tcggtcgacg
     2221 tgattcaacc tttgctcggc cggctgctcg actacggctc cgtccgggtg cgcggcacag
     2281 gcgcaagcct tgagcagctc aattatgtcg cccatccact cgcgttgcgc agtgcgatca
     2341 cggcgcgctg acgcgccatc tactcaccac tcttaggtat cgcgtgcagc gcggcattgc
     2401 ggatcgcatc cgcggccttt tccgccgcct caaaagcaga gcgcgtgatg tcctgaccgg
     2461 cctggaaatt ggccatcgct gcattattgg ccgcgtcgag cgcgtgattg aggcccgagg
     2521 ccgttttggc ggtgatgtcg gccggcggtg tggccgcccc gtttccaccg gcatcgacga
     2581 tatcgctgcg catctccgcc atgcgtgcgc ccatcaacct gatatgatcg ttaaatgctt
     2641 cgaacataga cgccatgaat ttggtctgga tcgaaatcag atcctcgacc gtgcgcgcgc
     2701 cctgaagatc atgcaggagc ttattgttgc gttcgaaatt ggaggcggaa atattgctga
     2761 tgtcctcggc cagcacttgc agcgacttga cgggcgagta gcccgtcggc cagccggcga
     2821 tcgaccctgc cttcgatctg ggttcgcgaa tctccatggt atcctcctat atggccgacg
     2881 ccgggaaacc cggcgccaac acatttttcg gaaccttagc ctatcgcgaa gtggagatcg
     2941 atagccgctt cggccagcgt gctatcgccg ccgcaggcca aagatcaggg aaacgatcaa
     3001 aaggatgatc gcaatccaga acaggacatg agcgacttcc gacgccgtgc ccgcaacgcc
     3061 gccgaagccg agcacaccgg cgacgatggc gataacaaga aagatcagag cccaatacag
     3121 catattgccg aaattcatcg cgcgctccct cgcccgatcg cgcgtcagcc caaaagccga
     3181 ccccggatcc cgcgcgatcg tgcggcagaa aatctggcaa ccgaggcgtg actttaacgg
     3241 cgcatgacac cgcgcggttc ccgcgaggct tgaggtcatt gcacgactta cgacagtgac
     3301 gtgcgaaagg ttacgcgggg tctgctgttt gctgcaacgc ggccgctata gcttccaacg
     3361 ctgcttgcgc gctgccgcca tccgggccgc cggcctgggc catatcgcgt cggccgccgc
     3421 cgcccttgcc gccaagctgc gcggctgcgg cgcgcacgag cgtcaccgca tcgaaacgcg
     3481 acgtcagatc atctgtgaca ccgacgacca cgccggcttt gccctcggca tcaatgccga
     3541 cgatggcgac gaccccagaa ccgagacttt gcttcgcctc atccgccagc gatttcagat
     3601 ccttcatttc gacacccgaa acttccttgc ggaagaactt tatgccgccg acctcctcga
     3661 tgggcgcatc cgcagcgccg ccgctcatcg caaggcgctt gcgcgcatcg ctcaattcgc
     3721 gttcgagctt gcgccgctca tcgaggacat gcgcgagacg cgcgccagcc tcgtcctctg
     3781 gcgccttaag cagatgcgcc atgtcctgaa agcgttgcgc ctggctgttc aaatggcggc
     3841 gtgcggcaag gccagtcttc gcttcaatac ggcggacgcc cgccgcgacc ggcgattccg
     3901 cgatgatcga gatgaggccg atatcgcccg tgcgccgcac atgggtgccg ccgcataatt
     3961 cgatggaaaa agcggcattg gcgcgggcgc cgcccgccgt ttccagcgga tcgcccatcg
     4021 agacgacgcg aacctgatcg ccgtactttt cgccgaacaa agcccgggcg ccggattcaa
     4081 tcgctgtatc gacatccatc agccgcgttt cgaccggcgc gttttgcagc acgacgcggt
     4141 tggcgatgtc ttccacctcg gcgagttctt cggccgagat cggcttaggg tgcgcgaagt
     4201 cgaaacgcag acgatccggc gcgacgagcg atcccttttg cgcgacgtga tcgccgagaa
     4261 cctggcgcag tgactcatgc agaagatgcg tcgccgaatg attggcgcgc acggcatcgc
     4321 ggcggctgtg atcgacgtca agctcgaccg aaagatcgac ggcggcatgg ccgctttcga
     4381 cggttccgtc atggacgaac agatcgccga gctttttgtg cgtgttggtc acgcggaagc
     4441 taagattcga cgcgcggatt gtccccgtat cgccgacctg accgccggac tcgccgtaga
     4501 acggcgtctg gttgaggatg acctgcccgg tttccccagc cgccagccgc cctacctcgc
     4561 ggccgtcctt gagcaatgcg acgatgacgc cttcggcact ttcggtttca tagccaagga
     4621 agtcggtcgc tccgacgcgt tctttgagcc caaaccagat cgactccgtc gcggcttcgc
     4681 ccgagcccgc ccaagccttg cgcgcgtcgg cgcgctgctg ctgcatcgca gtgttgaagc
     4741 cttcgacatc gaccccaagc ccgcgaccgc gcaacgcgtc ctgcgtcaga tcaagcggaa
     4801 agccgtaggt gtcgtagagc ttgaaagccg cggcgcctgg caacacgccg ccctccggca
     4861 gatcgcgggt ctcttcgtcg aggatcgtaa gtccgcgcgc caatgtgttg ataaagcgcg
     4921 tttcctctag ccggagcgtt tcgacgatca gcgcctcggc gcgaacgagt tcgggatagg
     4981 cctgccccat ttcacgcacc agcaccggta cgagccgcca catcacgggc tcgcgggcgc
     5041 caagaagctg cgcatgtcgc atggcgcgac gcatgatccg gcgcaccaca tagccgcggc
     5101 cttcgttcga cggcagaacg ccatcggcaa cgagaaacgc cgacgcgcgc aaatggtcgg
     5161 cgatgacgcg atggctcgcc ttttgcggcc cgtcgggatc aacgcccgtg gcttgcgcga
     5221 cggcaagcac cagggcccgc ataagatcga tatcgta
//
LOCUS       DASWOG010000321         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_264067,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000321 DASWOG010000000
VERSION     DASWOG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264067"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2837)
                     /gene="addB"
                     /locus_tag="VGB93_06625"
     CDS             complement(<1..2837)
                     /gene="addB"
                     /locus_tag="VGB93_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY0145812.1"
                     /translation="MPQKNIFTIAPGAPFLECFVGALLDGRIVANFSRALSALELADA
                     TIYVPTQRAARALADEFARALGRPATLLPRILPLGGLEATETDLLFSALGFAAPEASL
                     PQAAGDIWRRMQLARLVHIWAKALQGAVISIDANGRRDADPQEPCLVGTSAADAWHLG
                     GELAALIDEMIIEDVAWAQLDPLVLPDFDQYWRITLDFLNIAITEWPKILAERDLVDA
                     ARRQVLLVDAQIAQIRAGRTKGPIIAIGSTGTNRATARLLAALAAAPNGAVVLPGLDQ
                     NLDETAWRQIGATDVGDPGFGHPQAALRRLLSILEVPRAEVTELGAPTPLRATRSRIV
                     SEALRPADTTEEWTRFRASPAFSQIAVALQGVSLIEAADEREEALALAIVLREMLETP
                     GATAALVTPDRDMARRVGAELTRFGIEIDDSAGEPLNASATGSLALRAIDAAVRGEGA
                     DLVALLGHPMTRLGLSREEILERAPLFEIGVLRQERHAATFPTQAAAIAAYRAAAQGP
                     FAHPAQKALDDTDWAKIADLLDRIETALAPLKAVAGTQDLQTWIGAHRAALATIANED
                     GLRGDDAVALEDLFDELIQAQAPELRFDAQSYGIFLAQVMGERVLRRARRTHPRLKIF
                     GLLEARLMPADVILLGGLDETVWPPQAQSDAFLNRPMRQALGLTPPERRIGQTAHDFF
                     MAMGHERVILSRAKKRDGTPMVESRFVQRLAALAGDAFKKCRERGTYYLDLARVLDRP
                     HSETPPIKRPAPKPPLDLRPRRLSVTRIETLRRDPYAIYAEFCLNLRELPEPAEIGDR
                     RSIGTLLHKTLEDFGRRYAVGALPGDAAAILSTLLEANFKDEREDPDFAAFAWPRLQA
                     AAAFYLDFEARRRPNLAKIDVENKSVIEILLDDKTSFTLSATADRIEHRADGGVNLID
                     FKTGTPPGIKEVRIGFAPQLTLEAAMARRGA"
     gene            complement(2906..3274)
                     /locus_tag="VGB93_06630"
     CDS             complement(2906..3274)
                     /locus_tag="VGB93_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0145813.1"
                     /translation="MAQVTVTIAKHVYRVACGEGEEAHLQGLARQIDQRIEKLKKDFG
                     EIGDQRLTIMAAITIADELVEAKRRITELDVEILNLTSKETDRTSERDEWADRLADSI
                     GETAARVERVAQNLNTLSRS"
     gene            complement(3296..3577)
                     /locus_tag="VGB93_06635"
     CDS             complement(3296..3577)
                     /locus_tag="VGB93_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 domain-containing protein"
                     /protein_id="HEY0145814.1"
                     /translation="MTSANRLDHALKRLTVALDQLEAATERRLKTAAQRTDIEEEFAV
                     LQDDRARLAVELDGAVARSQSLELANNEVARRLAKARETIEEMISHVVM"
     gene            4094..>4720
                     /locus_tag="VGB93_06640"
     CDS             4094..>4720
                     /locus_tag="VGB93_06640"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0145815.1"
                     /translation="MAVNVAINGFGRIGRNVLRAIAESGRRDINVVAINDLGPVETNA
                     HLLRFDSVHGRFPGEVKVDGDTIDIGRGPIKVTAVRDPAELPHSALGVDIVLECSGVF
                     TSKAKASAHLKAGAKRVLVSAPADEADLTVVYGVNHDKLSPEHRVVSNASCTTNCLAP
                     VAKVLNDAVGIEHGFMTTIHSYTGDQPTLDTMHKDLYRARAASLSMIPT"
BASE COUNT          863 a         1439 c         1485 g          933 t
ORIGIN      
        1 aaagcgccgc ggcgagccat tgccgcttca agcgtcaatt gtggcgcgaa gccgatgcgt
       61 acctccttta tgcctggcgg cgtaccggtc ttgaagtcga ttaggtttac gccgccatcg
      121 gcgcgatgtt cgatgcgatc ggccgtggca gaaagcgtga aagacgtctt gtcgtcgagt
      181 agaatctcga tgacgctttt gttctcgaca tcaatcttcg caaggttcgg ccgccggcgc
      241 gcttcgaaat cgagatagaa ggcggcggcc gcctgcaggc gcggccaggc gaaggcggcg
      301 aagtcgggat cctcgcgttc atccttgaag ttagcttcga gcagcgtgga taaaatcgcc
      361 gcagcatcgc cgggtagcgc acccacggca taacgccgac cgaaatcttc caacgtttta
      421 tgcagcaatg tgccgatgct gcgtcggtcg ccgatttccg ccggttccgg cagttcgcgc
      481 aggttcaggc aaaattcggc gtagatcgcg taaggatcgc gccgcaacgt ctcgatccgc
      541 gtcacgctga ggcgacgtgg ccgcagatcg agcgggggtt tcggcgcggg ccgtttgatt
      601 ggcggtgttt cggaatgcgg gcgatcgagc acacgcgcga ggtcgagata atacgtcccg
      661 cgctcgcggc atttcttgaa cgcgtctccc gccaaagccg cgagcctctg cacgaagcgc
      721 gattcgacca tcggcgtccc gtcgcgtttt ttggcgcggc tgagaatgac gcgctcgtgc
      781 cccattgcca tgaagaaatc atgcgcagtc tggccgatcc gccgttccgg cggtgtcagg
      841 ccgagggctt gccgcatggg acggttcaag aaggcatcgc tttgcgcctg tggtggccac
      901 accgtctcgt cgagcccgcc tagcagaatg acatctgcgg gcatcaaccg ggcttcgagc
      961 aggccgaaaa tcttgagccg cggatgcgtg cgccgggcgc ggcgcaatac gcgttcgccc
     1021 atgacttgtg cgagaaagat gccgtagctc tgcgcatcga agcgcagttc cggcgcctgc
     1081 gcctgaatca attcgtcgaa aagatcttcg agcgcgacgg catcgtcgcc ccgcaggccg
     1141 tcttcattgg cgatggtggc aagcgcggcg cggtgcgcgc cgatccatgt ctgcaaatct
     1201 tgcgtgcctg ccacggcctt gagtggcgca agtgctgtct cgatccggtc aagcagatcg
     1261 gcgattttcg cccaatccgt gtcgtcgagc gccttttgcg ccggatgcgc gaaggggcct
     1321 tgtgccgccg cgcgataggc cgcgatggcc gctgcttgcg tcggaaacgt cgcggcatgg
     1381 cgctcctgcc ggagcacgcc gatctcgaac agcggcgcgc gttcgagaat ttcttcgcgc
     1441 gagagtccga gccgtgtcat cggatggccg aggagggcga caaggtcggc gccttcgccg
     1501 cgcacggcgg cgtcaatcgc ccgcagcgct aggctgccgg tggcgctggc gttcaagggc
     1561 tccccggcgg aatcgtcgat ctcgatgccg aaacgcgtca gttccgcacc gacccggcgc
     1621 gccatgtcgc ggtcgggcgt cacgagggcc gccgtcgcgc cgggcgtttc cagcatttcg
     1681 cgcaggacga tcgcgagcgc caaggcttcc tcgcgctcgt ccgccgcctc gatgagactg
     1741 acgccttgga gcgccaccgc gatctgcgaa aatgcaggcg acgcgcgaaa acgcgtccac
     1801 tcttcggtcg tatcggcggg gcgcaaggct tcagagacga ttcgtgagcg ggtggcgcgc
     1861 aacggtgttg gtgcgccaag ctctgtcacc tcagcgcgcg gcacctcaag gatcgacagc
     1921 aggcggcgca gcgccgcttg cggatggcca aagccgggat cgccgacatc tgttgcgccg
     1981 atctgtcgcc acgccgtctc atcgagattt tgatcgaggc cgggcaggac gacggcgcca
     2041 ttgggcgcgg cggccagcgc ggccagcagg cgcgcagtgg cgcgattggt gccggtcgag
     2101 ccgatcgcga tgatcgggcc tttggtccgc ccggcccgga tttgcgcgat ttgcgcatcg
     2161 accaggagaa cctgtcgccg cgccgcgtcg accagatcgc gttcggccaa aatcttcggc
     2221 cattccgtaa ttgcgatgtt caaaaaatcg agcgtgatac gccaatattg atcgaagtcg
     2281 ggcagaacga gcgggtcgag ctgcgcccag gcgacatcct cgatgatcat ctcgtcgatc
     2341 agggcggcaa gctcgccgcc gagatgccag gcgtccgcgg cggaggtgcc gacaaggcag
     2401 ggctcctgcg ggtcggcgtc gcgccggcca tttgcatcga tcgagatgac ggcgccctga
     2461 agtgcttttg cccagatatg gacgaggcgg gcgagctgca tccgccgcca gatgtcgccg
     2521 gctgcctggg gcaggctcgc ctccggcgca gcaaacccga gggcggagaa cagcagatcg
     2581 gtctccgttg cctcaaggcc gccgagcggc aggatgcggg gcaggagagt cgcggggcgc
     2641 ccgagcgcgc gggcgaattc gtcggcaagc gcgcgcgccg cgcgttgtgt cggcacatag
     2701 atcgtcgcat cggcgagttc gagagcgctc agggcgcggg aaaaatttgc gacgattcga
     2761 ccgtcgagca gcgcaccgac aaaacattca agaaatggcg cgcccggcgc gatggtgaag
     2821 atgttttttt gaggcatgat ttcgggcaca gccgctccgg cgccacgact cgcggcaccg
     2881 tagatcattc tgcccgaaaa accgtctagc tgcgactgag cgtgttgaga ttctgcgcca
     2941 cgcgctcgac ccgcgccgcg gtctcgccga tcgaatcggc cagccgatcc gcccattcgt
     3001 cgcgctcact cgtccggtct gtctctttcg acgtgagatt gagaatctcg acgtcgagtt
     3061 cggtgatccg gcgtttggct tcgacgagtt cgtcggcgat cgtaatggcg gccatgatgg
     3121 tcagccgctg atcgccgatt tcgccgaaat cctttttgag cttttcgatc cgctggtcga
     3181 tttgccgggc gagaccttgc agatgcgctt cttcgccctc gccacaggcg accctgtaaa
     3241 cgtgcttggc gatggtaacc gttacttgag ccatgacgca tacccccaca gacgcttaca
     3301 tcacgacgtg cgatatcatc tcttcgatcg tttcacgcgc cttcgcaagc cggcgcgcga
     3361 cctcgttatt ggccagttcc agagattgcg agcgtgcgac ggcgccgtca agctcaacgg
     3421 cgagtcgcgc ccggtcatcc tgcaggaccg caaactcttc ttcgatatcg gtgcgctgcg
     3481 cggccgtttt caaacgacgc tcggtcgccg cctcaagttg atccaatgca actgtaaggc
     3541 gcttcagcgc atggtctagt cgattggcac tcgtcatcga attctaatcc acgaactgct
     3601 gattaggcgc cggttcattt accgcgtcaa cgctggtgcc aacctattca cagtcaattc
     3661 acgtggacga accgatactt aaacgatatg cgacgggctt tctacgcggc gacgttgcgt
     3721 gcggtgcagc aatttggttt cgaccaattg ccaataactt gggcgtggca gtttgggcga
     3781 aacaaaacgc catcttggcg tgtttaagga cggcacggtt ccgcccacgc gttgaacgga
     3841 ggaagattga tgaaacccgc ggcactgcac gaagcaccga atacccccga ttcgccgccg
     3901 ggtttgatct taactatctg aaaaattttc atatttatct cacgacggcc cgctttgccg
     3961 cctgtggaca agacgaatca cgatcgattg gacaatttgc agacgaattg acttccaaac
     4021 cggcgctcat ctgcataaca agatggcgcc aacctaggcc tgcgctttat gcgctcaaaa
     4081 ggagcggacg tttatggcgg tgaatgttgc aatcaacggc tttggacgca tcggccgcaa
     4141 tgttctgcgg gccattgccg agtccggacg ccgggatatt aacgtcgtcg ccatcaacga
     4201 tctgggtccg gtcgaaacca atgcgcatct tttgcgtttc gactctgtgc atgggcgttt
     4261 cccgggcgag gtgaaagtcg atggcgacac catcgacatt ggccgcggcc cgatcaaggt
     4321 aacggcagtg cgcgacccgg ctgaactccc gcatagcgcg ctcggcgtcg acattgtgct
     4381 cgaatgttcc ggcgtgttca cgtcgaaggc gaaagcctcg gcgcatttga aggcgggtgc
     4441 gaagcgcgtt cttgtttcgg cgcctgccga tgaagcggat ctcacagtcg tgtatggcgt
     4501 caatcacgat aagctttctc cggagcatcg cgtcgtttcg aacgcctcct gcaccacaaa
     4561 ctgcctggcg ccggttgcca aagtgctcaa cgatgctgtt ggtatcgagc acggcttcat
     4621 gacgacgatc cattcctata ccggcgatca gccgacgctc gacacgatgc ataaggatct
     4681 ttaccgggcg cgtgccgcat ctctttcgat gattccgact
//
LOCUS       DASWOG010000322         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319087,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000322 DASWOG010000000
VERSION     DASWOG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319087"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VGB93_06645"
     CDS             <1..503
                     /locus_tag="VGB93_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145816.1"
                     /translation="GSVPEVEMAAVQVSARACRALFGVVAALVAASSCAAPFAAAARP
                     IIPAERRYDAYAGVLPTCADPGVVERIRSLFHDRETEFWKSGLEIRNLGNVRETGFRP
                     DGLDHIPRRYCEAQVYLNNQSVRPVSYSIVEDAGFIGFSYDVEWCVGGLDRNDAYGGN
                     CHDAQP"
     gene            500..1348
                     /locus_tag="VGB93_06650"
     CDS             500..1348
                     /locus_tag="VGB93_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HEY0145817.1"
                     /translation="MIFDSMRVSQFLAAIFVAGTCALLSSAAAAQESGCILDNCADKK
                     PLPPPQISPPSPQAPAPETPAAETPATNADSSGSSGGWNPGAPQPSGASTPGDFGFYV
                     LSFSWSPGFCALGGAEKSPDQCHDGVNLGFVVHGLWPQYNQGFPSDCGGATPSWLVLQ
                     SIQGLYPDVGLARYEWRKHGTCSGKSPTAYFADVRRAHDLIVIPPAFQNEQSEQEIAP
                     LDIQRAFFTANPRQRPGMISIECVNSELSEVRFCLSKDVRNFVPCPEVARQSCRAQDI
                     AVAPMR"
     gene            1359..2219
                     /gene="rlmJ"
                     /locus_tag="VGB93_06655"
     CDS             1359..2219
                     /gene="rlmJ"
                     /locus_tag="VGB93_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY0145818.1"
                     /translation="MNYRHAFHAGNFADVFKHVILTRILVYLALKETPFRVIDTHAGE
                     GFYDLSDERAAKTAEWRGGIGRLLEATPPPDIAALLAPYLAIAMPLLADVPPLYAGSP
                     ALALKLLRPQDRMIFCDAHPEVIASLRTQRAFSRDSRVKIVDIDGYQALNAFIPPRER
                     RGLIVIDPPFEAKTEFDDLKATLTLVVPKWPTGTYLVWFPVKDIGAVARFRSGFEAAL
                     PSLGVRSALYLKLAVDVPRPDGPLVSSGLIILNPPFTLAIEAQQILPYLAKTMANMDR
                     GDAEVAWLKH"
     gene            2321..2533
                     /locus_tag="VGB93_06660"
     CDS             2321..2533
                     /locus_tag="VGB93_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0145819.1"
                     /translation="MPQQGTVKFFNANKGFGFITPDGGGPDIFLHITAVERAGLSNLN
                     EGQRVSFETEPDKKGKGPKAVDLHIV"
     gene            2673..3275
                     /locus_tag="VGB93_06665"
     CDS             2673..3275
                     /locus_tag="VGB93_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0145820.1"
                     /translation="MMILRSAPASPFGRKVKIAADLLGLHDAIEVVFADTNDPNDSLP
                     TQNPLGKIPALILDDGSKLYDSRVIVEYLDALAGGGRLIPSASAARWRVLTAAALADG
                     MNDAALLIIYEKRFRSAELWSQAWMERQALKITRALAVFAAAPPAGIRDISHIGLACA
                     LGYLDLRFAGAWRGEHSALVGWLDAFAADVPAFEATRVKD"
     gene            complement(3272..>3864)
                     /locus_tag="VGB93_06670"
     CDS             complement(3272..>3864)
                     /locus_tag="VGB93_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY0145821.1"
                     /translation="PAPYYEPAPIFTWTGAYVGASAAFGFGRFTHDGGPYFGDASGGL
                     FGVQAGYNYQSGPLVAGIEGDINFGGVSGTNNPFPGATANGNINAEGSLRARFGYAFN
                     HTMVYITGGYTGANLKSSLTDFGISPNIYANESTYLNGWTIGAGVEYAITRNVSVKAE
                     YLYSNYGTGTLFSGTPDFARPGLSLSTLRAGVNYHF"
BASE COUNT          707 a         1173 c         1178 g          806 t
ORIGIN      
        1 ccggctcagt tccggaggtc gaaatggctg cagtccaagt ttccgcccgc gcgtgccgcg
       61 cattgttcgg cgtcgtagcc gcgcttgttg cggcgtcgtc ctgtgcggcg ccttttgcgg
      121 ccgccgcgcg gccgatcatc ccggcagagc ggcgctacga tgcctacgcg ggcgtcttgc
      181 cgacttgcgc cgaccccggc gttgtcgagc gcatccgctc gctgtttcat gatcgtgaga
      241 ccgaattctg gaaatccggc ctcgaaatcc ggaatctcgg caacgttcgc gagaccggct
      301 tccggcccga tgggctcgac catattccgc ggcgttattg tgaggcgcag gtctatttga
      361 acaatcagtc cgtgcgcccg gtttcctatt cgatcgtcga ggacgcgggc ttcatcggtt
      421 tcagttatga tgtcgaatgg tgcgtcggtg gacttgatag aaacgatgcc tatggcggta
      481 attgccacga cgcgcagccg tgatctttga cagcatgcgc gttagccagt ttctcgcggc
      541 gatcttcgtc gccggcacct gcgcattgtt atcgtccgcg gctgccgcgc aggaatccgg
      601 ctgcattctc gacaattgcg ccgacaagaa gccattgccg ccaccgcaga tatctccgcc
      661 gtcaccgcaa gcccccgctc ctgaaacccc cgcggctgaa acgcctgcaa cgaatgcaga
      721 cagcagtggt agcagcggcg gctggaaccc cggtgcgccg cagccgagcg gcgccagcac
      781 gcccggcgat ttcggtttct atgtcctgtc gttttcctgg tcgccgggtt tttgcgcgct
      841 gggcggcgcc gaaaagtcac ccgaccaatg tcacgatggc gtcaatctcg gctttgtcgt
      901 tcacggctta tggccgcaat acaatcaggg tttcccctcc gattgcggtg gcgccacgcc
      961 atcctggctc gtactgcaat cgatacaggg tctttatccc gatgtcggac ttgcccgcta
     1021 cgaatggcgc aagcacggca catgttccgg caaaagtccg acggcttatt tcgccgatgt
     1081 gcgccgtgca cacgatctga tcgtcatccc gcccgcgttt cagaatgagc agagcgagca
     1141 ggaaattgcg ccgctcgata ttcaacgcgc attcttcacc gccaatccac gccagcggcc
     1201 ggggatgata tcgatcgaat gcgtcaacag tgaattgtcg gaggttcgat tttgcctgtc
     1261 gaaggacgtg cgcaacttcg tgccgtgtcc ggaggtcgcg cgtcagtcct gccgggcgca
     1321 ggatatagct gtcgcgccga tgcggtaagc gaccacggat gaactaccgc cacgcctttc
     1381 acgccggcaa tttcgccgac gtcttcaaac acgtcatcct gacgcgcatt cttgtttatc
     1441 tcgcgctgaa agagaccccg ttccgcgtca tcgacacgca cgcgggcgaa ggcttttacg
     1501 atctctcgga tgaacgcgcc gcgaagacgg cggaatggcg cggcggaatc ggccggctgc
     1561 ttgaggcgac accgccgccc gacatcgcgg cgcttcttgc gccttatctt gcgattgcca
     1621 tgcccttgct cgccgatgtg ccaccgcttt atgccggatc gcccgcgctc gctttgaagc
     1681 ttttgcggcc gcaggaccgg atgatctttt gcgatgcaca tcccgaggtg attgcgtctt
     1741 tgaggacgca gcgtgcgttt agccgcgatt ctcgggtcaa gatcgtcgat atcgatggct
     1801 atcaggcgct caacgctttc attccgccac gcgagcgccg cggccttatc gtgatcgatc
     1861 cgcccttcga agcgaagacg gaattcgacg atctcaaagc cacgctcacg cttgtggtgc
     1921 cgaagtggcc aacggggaca tatctcgtgt ggtttccggt caaggatatc ggcgccgtcg
     1981 cccgattcag gtccggcttc gaagcggcgt tgccgagtct tggcgttcga agcgccctgt
     2041 atctgaaact cgcggttgac gtgccacggc ctgatgggcc gctcgtgtcc agtggcctga
     2101 taattctcaa tccccccttc acacttgcca tagaagcgca gcagattttg ccgtatcttg
     2161 cgaaaaccat ggccaacatg gatcggggcg acgccgaggt cgcatggctc aagcactgac
     2221 cgaaaggctg gcttgcggac catgcggtaa taggcttttg cggcgctgcg aagaaataat
     2281 cgtcggtcaa agcatcgacg tggaagtcag ggagagaaat atgccgcagc agggaactgt
     2341 gaaatttttc aatgcgaaca agggctttgg cttcatcacc ccggacggcg gcggccctga
     2401 tattttcctt catatcaccg cagtggaacg cgccggcctg tcaaatctca acgaagggca
     2461 gcgggtaagc tttgagaccg agcccgacaa gaagggcaaa ggccccaagg ccgtcgacct
     2521 gcacatcgtc tagcgcgcca gcctttgcac cgccgctcgc gagagcggcg ggacatggcg
     2581 gtctaacctt tattaggacg ttaaccccgg ggtatgtgcc atgccggcat gtacctcgtt
     2641 gcgcgttttg gggcattgaa ggaagtggac cgatgatgat tttgcgttcg gcgccggcct
     2701 caccgttcgg gcggaaggtg aagatcgccg ccgatctgct cggcctgcac gatgcgatcg
     2761 aggttgtttt cgccgatacc aacgatccga acgattcgct gccgacgcaa aatccgctcg
     2821 gcaagattcc cgcgctgatc ctggacgacg gcagcaaact ctacgatagc cgcgtcatcg
     2881 tcgaatatct cgatgcactc gccggcggtg gcaggctcat cccatctgcg tcggcggcgc
     2941 gctggcgcgt cctgaccgct gcggcgctcg ccgatggtat gaacgacgcg gcactcctga
     3001 tcatctacga gaagcggttc cggtcggccg agctttggtc gcaagcttgg atggagcgtc
     3061 aggctttgaa gatcacccgc gcgctggcgg tctttgctgc cgcgccgcct gccggcattc
     3121 gcgacatctc tcacatcggt cttgcctgcg cgcttggtta tctcgacctg cgcttcgcgg
     3181 gtgcctggcg cggcgaacac tcggcgcttg tcgggtggct cgatgcgttc gcggcggacg
     3241 tgccagcctt cgaggcgacg cgcgtcaaag actagaagtg atagtttaca ccggcgcgca
     3301 gcgtcgaaag gctcagaccg ggacgcgcaa aatcgggcgt tccgctgaac agcgtgccgg
     3361 tgccgtaatt gctgtaaagg tattcggcct tgacggagac attccgcgtt atggcgtatt
     3421 ccacacccgc gccgatcgtc cagccgttca aataggtcga ttcattggcg tagatatttg
     3481 gcgagatacc gaaatcggtg agcgagctct tgaggttggc gccggtataa ccgccggtga
     3541 tgtagaccat cgtgtggttg aaggcatagc cgaagcgggc gcggagtgag ccttcggcgt
     3601 tgatgttgcc gttcgcagtg gcacccggga agggattgtt tgtgccgctg acgccgccaa
     3661 aattgatgtc gccctcgata ccggcgacca gcgggcctga ttgataatta tagcccgcct
     3721 gaacaccgaa gagaccacca gatgcatcgc cgaaataggg gccgccatcg tgcgtgaatc
     3781 ggccgaagcc gaaggccgca ctggcgccga cataggcgcc cgtccaggtg aagatcggtg
     3841 ccggctcata ataaggcgcc ggcg
//
LOCUS       DASWOG010000323         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_7377, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000323 DASWOG010000000
VERSION     DASWOG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7377"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VGB93_06675"
     CDS             complement(<1..490)
                     /locus_tag="VGB93_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0145822.1"
                     /translation="MSEPFVTRNDITSWGRVVREPQHVATPRFLDQLTPLVDAARNSS
                     LLASGLRRSYGDSCLNGHGDLIDMSKLDRIIRFDSANGILRAAAGASFSAILQLIVPH
                     GWFLPVTPGTRFVTLGGAFANDVHGKNHHTAGTFGRHVRAIGLLRSDRGRLTLTPQTE
                     PSL"
     gene            complement(706..1149)
                     /gene="rnhA"
                     /locus_tag="VGB93_06680"
     CDS             complement(706..1149)
                     /gene="rnhA"
                     /locus_tag="VGB93_06680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0145823.1"
                     /translation="MSEKITIWTDGACSGNPGPGGWGAIMTFGDHRKELSGGDAATTN
                     NRMELTAAIRALEALKRPCTVDLYTDSAYLRGGIMTWLAGWKKNGWRTADKKPVKNIE
                     LWQELEQAAAPHKIVWHWLKGHAGDPMNERADELAREGMRPFLRK"
     gene            complement(1146..2111)
                     /gene="thrB"
                     /locus_tag="VGB93_06685"
     CDS             complement(1146..2111)
                     /gene="thrB"
                     /locus_tag="VGB93_06685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0145824.1"
                     /translation="MAVYTEVSDTALAGFLADYDLGQLLAYKGIAEGVENSNFLLHVA
                     AGFFILTLYEKRVRASDLPFFLGLMEHLSARGLVCPQPVKTQRGALLGTLAGKPAAIV
                     TFLEGVSTRMPTAAHCAALGAALGQLHLAGADFPHERTNDLSMSAWPNLFAQAEDRAD
                     SVEPGLADFLATELSALAGLWPQGLPRGIIHADLFPDNVLFLGADVSGLIDFYFACTD
                     FLAYDLAICLNAWCFEADFSFNITKGKALIDAYQACRRLTDEEAAALPVLARGAALRF
                     TLTRLVDWLNVPSGALVRPKDPREYARRLRFHQQTTGAQDLGLTR"
     gene            complement(2501..3634)
                     /gene="tgt"
                     /locus_tag="VGB93_06690"
     CDS             complement(2501..3634)
                     /gene="tgt"
                     /locus_tag="VGB93_06690"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0145825.1"
                     /translation="MTASLTFEVHDTDGEARNGTLSLPRGAIRTPAFMPVGTAATVKA
                     MFPQDVHALGADIVLANTYHLMLRPGAERVAALGGLHKFMKWQWPILTDSGGFQVMSL
                     SKLRKLDESGVTFQSHIDGARHHLTPERAMEIQRLLGSDIQMQLDECVKLPAPESEVE
                     RAMQLSLRWAERCKIAFGTQPGHALFGIVQGGASPALRRASAEALVAMGFDGYALGGL
                     AVGEPQEVMLAMIEASITHLPGERPRYLMGVGSPDDLIESVRRGIDMFDCVMPTRAGR
                     HGLVYSRFGRINLRNAKHADDSRPIDPDAPCEAARVYSRAYLHHLVKAGEILAMMLLT
                     TINLAYYETLMAEVRTAIAGGRYADFCAETKAKWSRGESARTP"
     gene            complement(3631..>4599)
                     /gene="queA"
                     /locus_tag="VGB93_06695"
     CDS             complement(3631..>4599)
                     /gene="queA"
                     /locus_tag="VGB93_06695"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0145826.1"
                     /translation="GESELFKDRTIRDLPDFLRPGDILVVNNTRVIPARLEGVRSRGT
                     TQAKIEVLLHKRVSANTWRAFAKGAKKLQRGDTIVFARDKAPTLEAEVLENFGEGEVL
                     LAFALAGDELDRAVEKLGAMPLPPYIAGKRPADAQDADDYQSLFAIHHGAVASPTASL
                     HFMPGLKTAIAKRGVDIAEITLHVGAGTLLPVKSDDTNAHNMHAEWGEIDAAIAARLN
                     AARAAGGRIVALGTTSLRLLESAATADGQIAPFSGDTAIFITPGYRFKAVDLLLTNFH
                     LPRSTLFMLVSAFSGLETMRAAYQHAIASGYRFYSYGDACLLVPQS"
BASE COUNT          954 a         1309 c         1470 g          866 t
ORIGIN      
        1 ataaggaggg ttcggtttgc ggcgtcagcg tcagccggcc gcgatcgctg cgcagcaggc
       61 cgatggcgcg gacgtgccgg ccaaaggtcc cggctgtgtg atggtttttg ccatggacgt
      121 cattggcaaa ggcgccgccg agtgtgacaa agcgcgtccc cggcgtgacg ggaagaaacc
      181 agccgtgtgg gacgatgagt tgcaggatcg cagaaaagct tgcgccagct gcggcgcgta
      241 gaattccgtt ggcggagtcg aagcggatga tgcgatcgag cttcgacatg tcgatcaggt
      301 cgccatgccc attgagacag gaatcgccgt aggagcgccg caggcccgag gcaagcaggc
      361 ttgagtttcg tgccgcgtcc accagaggag tgagctgatc gaggaaacgc ggcgtcgcaa
      421 cgtgttgcgg ctcacgcacc acacggcccc acgacgtgat gtcgttgcga gtaacgaaag
      481 gttcgctcac gttcggactt tcctgatcag agaggcatca atccttcgca cacacggaag
      541 ggcgatgcga accggaaatc tcatcgcgcg caatccagct tcgccgttga atatatctta
      601 gcgcgacaag agagaaaacc gcgagaagat aagttcggag aaaataccgc tctcgcgagt
      661 tcagcgagca ttagcgccgt cttgttacga tcatatgcca gccggctact ttcgcagaaa
      721 tggccgcatt ccctcgcgcg ccaattcgtc ggcgcgctca ttcatcgggt cgccggcatg
      781 acctttgagc cagtgccata cgatcttatg cggcgcggcg gcctgttcga gttcctgcca
      841 gagttcgata ttcttgaccg gctttttatc ggccgtgcgc cagccgttct tcttccagcc
      901 cgcgagccaa gtcatgatgc cgccgcgcag ataggcggaa tccgtataga ggtcgacagt
      961 gcaaggccgc ttcaaagctt cgagggcgcg gatcgcggcg gtgagttcca tacgattatt
     1021 ggtcgtcgcg gcgtcgccgc cggaaagttc cttgcgatga tcgccaaaag tcatgatggc
     1081 gccccagccg ccggggccgg gattgccgga acaggcgccg tccgtccaga tcgtgatctt
     1141 ttcactcaac gcgtcaatcc gagatcctgc gcacccgtgg tctgctggtg aaagcgcagg
     1201 cggcgcgcat attcgcgcgg atctttcggc cggacgagag cgccggatgg aacattcagc
     1261 caatcgacga ggcgcgtgag agtgaaccgc aaagcagcac cgcgggcaag aacaggcaag
     1321 gcagcggctt cttcatcggt caatcggcgg caggcctgat aggcgtctat cagcgccttt
     1381 cccttggtga tgttgaacga aaaatccgct tcgaaacacc aagcgttgag acagatcgcg
     1441 agatcataag cgagaaaatc ggtgcaggcg aaatagaagt cgatcagccc tgagacatcg
     1501 gcgccgagga atagaacatt gtccggaaag agatcggcgt gaatgattcc gcgcggcagg
     1561 ccctgcggcc aaagtccggc aagcgccgag agttcggttg cgagaaagtc agcgagcccg
     1621 ggctcgacac tatcggcgcg gtcttccgct tgggcgaaaa ggttgggcca cgccgacatt
     1681 gagagatcgt tcgtgcgctc gtgcggaaaa tcagcgcccg cgagatgcaa ttgtcccagc
     1741 gctgcgccga gcgccgcaca atgcgcggct gtcggcattc gcgtcgaaac gccttcgaga
     1801 aaagtcacaa tggccgcggg cttgcccgcg agcgtgccaa gcagggcacc ccgctgggtt
     1861 ttcaccggct gcgggcagac gaggcctcgc gcggaaagat gttccatgag gccgaggaag
     1921 aacggcaggt cgctggcgcg gacgcgtttt tcgtaaagcg tcaggatgaa gaatccggcg
     1981 gcgacatgca gcagaaaatt ggaattttcg acgccttcgg caatgccttt ataggcaagc
     2041 aattggccga ggtcgtaatc ggcgagaaag cccgcgaggg ccgtgtcgga aacttcggta
     2101 tagacagcca tgagcgtgtg cgcgactcgg aataagcggc caacttgccc gcaggtttag
     2161 cacttggaaa ggggagagaa gatcgtaggg gagccgcaag tcttggttgg tatttttacc
     2221 tggaagggga gaataaaaaa gaatttttat gccttttttg agagcaggat gacgcattgc
     2281 tgcgtgacaa tgcgtcacgc gtgagctaca aacgcccacc gtccagttga gagcgttcga
     2341 gttctcctga acgggtacgt agtccaagtc gtgggaggaa cccctttcgc atcgtctgcg
     2401 tggaaacgcg ctatccccaa acgggaacag acgggaaagg gtctagggcg agggagcgga
     2461 aaggcgaagt cgggcccttg gcctgcttcg ccttttgcgt ttatggggtg cgcgccgact
     2521 cgccgcgtga ccatttggcc ttcgtttccg cacagaagtc cgcatagcgc ccgccagcga
     2581 tcgcggtgcg aacctcggcc atcaacgttt cgtaataggc aagatttatc gtcgtcagca
     2641 gcatcatggc gagaatttcg ccggctttca cgagatggtg cagatacgcg cgggaatata
     2701 cgcgtgctgc ttcacaaggt gcgtcaggat cgatgggccg cgaatcgtcg gcatgttttg
     2761 cgttgcgcag attgatccgg ccgaagcggc tgtagacgag gccgtggcgg ccggcgcgcg
     2821 tcggcatcac acaatcaaac atgtcgatgc cccgccgcac ggattcgata agatcgtccg
     2881 gcgagcccac gcccatcaga tagcgcgggc gctcgcccgg cagatgcgta atgcttgcct
     2941 cgatcatggc cagcatcacc tcttgcggct cacccacggc gaggccaccg agcgcatagc
     3001 cgtcgaagcc catggcaacc agtgcctcgg cactcgcgcg ccgcagcgcc ggacttgcac
     3061 cgccctggac aatgccgaag agagcatggc cgggttgcgt gccgaacgcg atcttgcatc
     3121 gctccgccca gcgcaacgaa agctgcatgg cacgctcgac ttcggattcc ggcgcgggca
     3181 gcttcacaca ttcatcgagc tgcatctgga tgtcggagcc gagcagccgc tgaatctcca
     3241 tcgcgcgttc aggtgtcaga tgatgccgcg cgccgtcaat gtgagattga aaggtcacgc
     3301 cgctttcgtc gagcttgcgc agctttgaga gcgacatgac ctgaaagccg ccggaatccg
     3361 tgaggatcgg ccattgccat ttcatgaact tatgcaggcc gcccagtgcg gcgacacgct
     3421 ccgcacctgg ccgcaacatc agatgatagg tattggcgag tacgatatcg gcgccaagcg
     3481 catgaacatc ctgcgggaac atcgccttga cagtcgcggc tgtgccgacc ggcatgaagg
     3541 cgggcgtgcg gatcgcgccg cgcggcaagc tcagcgtgcc attgcgggct tcgccatccg
     3601 tgtcgtgaac ttcgaaggtc aatgatgccg tcatgattgc gggacgagca ggcaggcgtc
     3661 gccataagaa tagaaacgat agccgctggc aatcgcgtgt tggtaggcgg cacgcatcgt
     3721 ttcgaggccg ctaaacgctg agacaagcat gaacagcgtc gaacgcggca ggtggaaatt
     3781 ggtgagcagc agatcgacgg ctttgaagcg atagcccggc gtgatgaaaa ttgcggtatc
     3841 gccggaaaag ggcgcgatct gaccatcggc cgtcgccgcg gattcgagca ggcgcaacga
     3901 tgtggtaccc agcgcgacga tgcggccgcc ggctgcgcgc gccgcgttca gccgcgccgc
     3961 tatcgccgcg tcgatctcgc cccattcggc atgcatatta tgagcgtttg tatcgtccga
     4021 tttcacaggc agaagagttc cggcgccgac gtggagggtg atctccgcga tatcgacgcc
     4081 ccgcttggct atcgccgttt taagtcccgg catgaaatgc aaactcgcgg ttggcgatgc
     4141 gacggcgccg tgatggatcg cgaagagact ttgataatcg tccgcatctt gcgcatcggc
     4201 cgggcgcttg ccggcaatat agggcggcaa aggcatcgcg ccgagctttt cgacggcgcg
     4261 atcgagttcg tcgccggcaa gtgcgaacgc aagcaagact tcgccttcgc cgaaattttc
     4321 gaggacttcg gcttctaggg tcggcgcttt atcgcgcgcg aagacgatcg tgtcgccgcg
     4381 ttgcagtttc ttggcgccct tcgcaaacgc gcgccatgtg ttggcggaaa cgcgcttgtg
     4441 cagcagcact tcgatcttgg cttgtgtcgt gccgcggctg cgcacacctt cgagccgggc
     4501 ggggatgacg cgtgtattgt tgacgacaag gatatcgccc gggcgcagaa agtccggcag
     4561 gtcgcggatc gtccgatctt tgaacaattc agattcgcc
//
LOCUS       DASWOG010000324         9247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319223,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000324 DASWOG010000000
VERSION     DASWOG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319223"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGB93_06700"
     CDS             <1..486
                     /locus_tag="VGB93_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145827.1"
                     /translation="GFAPIDQRGFFAPDRTRVARDRPNVPVVFIQLGDTKTWAQIDTG
                     YDDDLYPTSLDINEPFYNELLASGVALTPEGFVTVKTCAGFESRPIYSSAQSLVITDE
                     AGLPVTETRKYYLIVKPVNMCGGIANRATPAAQLGSSFMNLFQSIVFDPNGQMVWLKK
                     N"
     gene            524..841
                     /locus_tag="VGB93_06705"
     CDS             524..841
                     /locus_tag="VGB93_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145828.1"
                     /translation="MIRTFALTAILFASQIFAWPAFAAQQLMPAPYPGQLGGNAQESQ
                     PSGSGGGAHQELVPEPAPQAKGPDALDDPRPITGSEHQQMAPHPYRRQDWCGGASSCS
                     IER"
     gene            complement(828..1391)
                     /locus_tag="VGB93_06710"
     CDS             complement(828..1391)
                     /locus_tag="VGB93_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0145829.1"
                     /translation="MLQKIGRGATSRYNAPAQLLHWLTALVIFTTIPLGWIIAHMGRD
                     APNHHVFIFFHKSFGMLALALIIARLVWRGTRKPPPLPDWIAKWEIGLAHATHVFLYL
                     IFIVMPVTGYIASSAGGHAVSFFGLPLPLLPEDKGLAKTANDIHLAGQYLVYFFLAAH
                     LVGVVWHVAFRKDGYLARMLPDQINAR"
     gene            complement(1491..2714)
                     /gene="zigA"
                     /locus_tag="VGB93_06715"
     CDS             complement(1491..2714)
                     /gene="zigA"
                     /locus_tag="VGB93_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HEY0145830.1"
                     /translation="MTTAIDERLPVTVLSGFLGAGKTTLLNHVLNNRTGRGVAVIVND
                     MSEVNIDVDLVREGGANLSRTDEKLVEMTNGCICCTLRDDLLAEVRRLAEDKQFDYLL
                     IESTGISEPLPVAATFDFRDEDGRSLSDLARLDTMVTVVDAVNLLKDYASADFLKDRG
                     ESLGEEDERVLVDLLVDQIEFADVVILNKTDVASAEDLDAARKIVRALNPDADLVETT
                     MAKVDLGRVLDTRRFNYEKAQQHPLWYKELYGFADHKPETLEYGIASFVYRARRPFHP
                     GKFHKFINSSWPGVIRAKGHFWLATRPDWCGELSQAGALVRNEAMGFWWAAVPKNRWP
                     DHPEWRESVAKNWDKVYGDRRQEIVFIGASMDEATIRKELDSCLVGDGNAREMPLAAW
                     KRLPDPFPAWKTAER"
     gene            complement(2841..3860)
                     /locus_tag="VGB93_06720"
     CDS             complement(2841..3860)
                     /locus_tag="VGB93_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY0145831.1"
                     /translation="MCRRLLPFAAVAALFLDLAGCSNYQAQRPAWRAEAENACLAENL
                     VRPSAYVRPVESIDGPGICGLTHPFKVAALLDGNVMLDGDYILDCPMIAALNAWVRDV
                     VEPAAQTRYNERVVEIETFGTYSCRGINGEFGARLSEHAFGNAIDVRGFRLQDGRNIL
                     IVHDWTMGDPQAQAFLRDVDNGACGDFTTVLGPGYNFLHYNHFHLDLAMRGNMSTGPR
                     RVCKPHPDVWPQTHAPDGLPEPPPVDEEMDVSQAGSGADQPVALHTGPGSLDAALPQS
                     DYTPPAPYSPPHSYAHSYQALSSDALPPEPVSPSQNATPQRGFMRADGAFVPEGRPAD
                     WDMQQ"
     gene            3966..4985
                     /locus_tag="VGB93_06725"
     CDS             3966..4985
                     /locus_tag="VGB93_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY0145832.1"
                     /translation="MDDTPATSFPRASGAQTMPGLVWAFRMHADGTPQSLPVDKPIDI
                     NDDGWLWLHFNLADARACALLPRFEDIPPEAAVALTAPDGAQQLHVEDTCVYGIFADL
                     IADLDGATNHFGYLHFAMTDRLLVSARRRAMNAAESARQALDRGHKLHRVSSLFELIV
                     NHVADSIDKYSDQLDEDMDDIEDELWQRAATDQRAKLAKVRKGAVHLHRQMQGLLALF
                     ERVGHRLDTAPPPELRLKTSILMQRLENLDRDIIAMRERAHMLQEEVALKIAEDTNRS
                     LYLLTIITTFFLPASLVAGIFGMNVKNLPFTDISSGFVWSMVILVGASLAILIFMQRM
                     RILLR"
     gene            5015..5437
                     /gene="arfB"
                     /locus_tag="VGB93_06730"
     CDS             5015..5437
                     /gene="arfB"
                     /locus_tag="VGB93_06730"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY0145833.1"
                     /translation="MARIAIAKNLSLDENEIEFTFLRASGPGGQNVNKVETAVQLRFD
                     VRNSPSLDDYVKRRLEKLAGSRLTLDGILIISAQRFRSQERNREDALVRLGALIAKAS
                     ERVKRRIATRPTFASQQRRRDAKQKRGEIKKLRGAPSD"
     gene            5511..6032
                     /locus_tag="VGB93_06735"
     CDS             5511..6032
                     /locus_tag="VGB93_06735"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY0145834.1"
                     /translation="MRVAFVTATALAAIFATAAAADDYPPRKPGLWEIAVHNQSLPDV
                     TMKMCIDAETDQLFHKFSGDIRSKHCSKSGLKTVGNVVTSESDCTLGGTKVDSVAVAT
                     FTGDSAYHVDIKTHFDPPKLGHSDVAMSQDAKWVSECPADMKPGDFDMGHGIKMNIKT
                     VERLRSLLPGHSD"
     gene            complement(6127..6696)
                     /locus_tag="VGB93_06740"
     CDS             complement(6127..6696)
                     /locus_tag="VGB93_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEY0145835.1"
                     /translation="MCVTRSFDRAPNGASMGLPTLHDWRPIEIVAMVLGFILYWPIGL
                     AILIAKIWQKKSAYAGDLPGFVSAKMQQKFREKWEQKMGRHGFGGMRRQDFYNWAYAG
                     AGERRGAGGPPWQWKPSSTGNLAFDEWREAELTRLEAERERLRAAEQEFTSFVENLRR
                     AKDREEFDRFMSQRNNQPPQNGAPESPAS"
     gene            complement(6806..7453)
                     /locus_tag="VGB93_06745"
     CDS             complement(6806..7453)
                     /locus_tag="VGB93_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0145836.1"
                     /translation="MIHMKRNWKHGGHFGPERRGYHHGGLKEALVEAARALMAEHGPG
                     GFTLAEAAKRVGVTGAAPYRHFADRQDLMGELARRGFDLFGERLAGAWDEGRPEPRVA
                     LKRMGRAYLNFATAEPGLYTAMFDNAHSLQGRAVHFVANKAFDGLTRAAAGVILTGGG
                     DPADIRRLALEIWALAHGTAMLTLAGHFDSARGDDPAAILDRGAEALIATALHRK"
     gene            7524..7805
                     /locus_tag="VGB93_06750"
     CDS             7524..7805
                     /locus_tag="VGB93_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145837.1"
                     /translation="MLSFIRLLFVCIIFAAPAFAAEQASPVPFQNKSAPTKVPSDVSV
                     LAYGAAHPECLEWTDSCRTCMRSPDGRVGCSTPGIACQPKEIVCKKESK"
     gene            7881..8774
                     /locus_tag="VGB93_06755"
     CDS             7881..8774
                     /locus_tag="VGB93_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HEY0145838.1"
                     /translation="MREKRLSAWVAARGPFIRFCYEFLRFGLKQGWACLFGGAMVALL
                     TGTHLFYPTHAPLARYDFLVVAAIVLQAVMLASRLETFDEFKVILLFHAVGTGMELFK
                     TATGSWLFPEPGFLRIAAVPLFTGFMYGTIGSYIARAWRLFDFHFRHHPPLWATNVLA
                     IAIYLNFFADHYGIDFRYALFAGAIVLFARTKVFFKVWRTYRAMPLLLGFFLVALFIW
                     FAENAGTAAHAWLYPNQRAGWSLVAIWKLGSWFLLMIISYIMVAWINRPRAFAPELDG
                     RIVDSNFVDSYKGLSADETSN"
     gene            complement(8785..>9247)
                     /locus_tag="VGB93_06760"
     CDS             complement(8785..>9247)
                     /locus_tag="VGB93_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0145839.1"
                     /translation="AKIYFATNRRIRGLWQTTIPSRFLDELPEAHVEVVDSASGRSYG
                     NYAQSRFANMDTYRSSYATPGWQRAQRRNAEETQQPGFSRDSAARRGPMTIDGELVAK
                     SSAASSFNKGARVFHMKFGPGTVAAVDGNKLTVDFDKAGRKMVLESFVQAA"
BASE COUNT         1858 a         2824 c         2722 g         1843 t
ORIGIN      
        1 ggtttcgcac cgatcgatca gcgcggcttc ttcgcacccg atcgcacgcg cgttgcgagg
       61 gaccggccca atgtgccggt cgtcttcatc cagctcggcg acacgaagac ctgggcgcag
      121 atcgacacag gttatgacga cgatctctat cccacttcgc tcgacatcaa cgagccgttt
      181 tacaacgagt tgctggcaag cggcgtggcg ctgacgcccg aaggctttgt gacggtcaag
      241 acctgcgccg ggttcgagag ccgcccgatc tattccagcg ctcaatcgct tgtgatcacc
      301 gatgaggccg gcctacctgt taccgagacg cgtaaatatt atctcatcgt caagccggtt
      361 aacatgtgcg gaggcatcgc caaccgggcg acgccggcgg cgcaacttgg cagttctttc
      421 atgaacctat tccagtccat tgtcttcgat cccaatgggc agatggtctg gctcaagaaa
      481 aactgaagca tgcacttcac cgctctcgat gaggagcgcg atcatgatca gaacattcgc
      541 gctgaccgcg atcctgttcg cctcgcaaat cttcgcttgg ccggccttcg ccgcgcagca
      601 attgatgccc gcgccctatc ccggccagct cggcggcaat gcgcaggaat cgcagccgtc
      661 tggatcaggc ggcggcgcgc atcaggaact cgtgcccgaa cccgcaccgc aggccaaagg
      721 ccccgacgca ctcgacgatc cgcggccaat tacaggcagc gagcatcagc agatggcgcc
      781 acatccttac cggcggcagg attggtgcgg cggcgcgagt tcctgctcta tcgagcgttg
      841 atctgatcgg gcagcatccg cgccagatag ccgtctttgc ggaaggcgac atgccacaca
      901 acgccgacaa gatgcgcggc caaaaagaaa taaacgagat attggccggc gagatgaata
      961 tcgttcgccg tctttgccaa gcctttgtct tccggcaata acggcaacgg cagcccaaag
     1021 aacgagacag cgtggccgcc ggcgcttgaa gcgatatagc ctgtcaccgg catgacgatg
     1081 aagatcagat agagaaaaac gtgcgtcgcg tgcgcgaggc cgatctccca tttggcgatc
     1141 caatccggca gtggcggcgg cttgcgcgtc ccacgccaga cgagccgcgc gatgatcagc
     1201 gcgagcgcta gcatcccgaa ggatttgtgg aagaagatga acacatgatg gttcggcgcg
     1261 tcgcggccca tatgcgcgat gatccagccg agcggaatcg tcgtgaagat gacaagtgct
     1321 gtcaaccaat ggagaagctg cgcgggcgcg ttatagcgac tcgtcgcgcc acgcccgatt
     1381 ttttgcaaca tgaagaacgc cttttggaat tgcgattgaa gcgtccttta gcccggcggc
     1441 cggcgtaacg ggaagagcgc aacgtcgcac cgccgcagaa tgtgaacggc ctatcgctcg
     1501 gccgtcttcc aggccggaaa cgggtcgggc agccgtttcc acgccgccaa aggcatctcc
     1561 ctggcattgc catcgccaac gaggcaggaa tcgagctcct tgcggatagt ggcctcgtcc
     1621 atgctcgcgc cgatgaacac gatctcctgc cgacggtcgc cataaacctt gtcccaattt
     1681 ttggcgacgg actcgcgcca ttcgggatga tccggccatc gattcttggg cacggccgcc
     1741 caccagaagc ccatcgcttc attgcgcaca agagcgcccg cctggctcag ttcgccgcac
     1801 cagtccggcc gagtcgcgag ccagaaatgt cccttggcac ggatgacgcc cggccagctc
     1861 gaattgatga acttgtggaa ttttcccgga tggaacggcc ggcgtgcgcg atagacgaag
     1921 ctggcgatgc catattccaa agtctccggt ttgtgatcgg cgaaaccgta aagttctttg
     1981 taccagagcg gatgctgctg cgctttctcg taattgaagc ggcgtgtatc gagcacgcgg
     2041 ccaagatcga ctttggccat tgtcgtttcg accaaatccg catcggggtt gagcgcccgc
     2101 acgatcttgc gcgccgcatc gaggtcttca gccgaggcga catcggtctt gtttaggata
     2161 acgacatcgg caaattcgat ctgatcgacc agcagatcga ccaaaacgcg ttcgtcctcc
     2221 tcgccaagcg attcaccacg atccttgagg aaatcggctg acgcataatc cttgagaagg
     2281 ttcacggcgt cgacgaccgt aaccatcgtg tcgaggcgcg cgagatcgct gaggctgcgg
     2341 ccgtcttcgt cgcggaagtc gaaggtcgcg gcgaccggca atggttcgga aatgccggtc
     2401 gattcgatca gcaaataatc gaactgcttg tcttccgcca aacgccgtac ctcggcgaga
     2461 agatcatcgc gcagcgtgca gcagatgcag ccgttggtca tctcgacgag cttttcgtcg
     2521 gtgcgcgaga gattggcgcc gccttcacgc acgagatcga cgtcgatatt cacttcgctc
     2581 atatcgttga cgatgacggc aacgccgcgg ccggttcgat tgttgagcac atggttgagc
     2641 agcgtcgtct tgccggcgcc gaggaagccg gaaagtacgg ttacaggaag tctttcgtcg
     2701 atcgccgtcg tcatccatca ccattgattc atattcgccg cgcgcctgcg cggcctgtcg
     2761 ctccgttttc gagcgacgga gatatgtaac aatataacat atctagatgc aagtcaggaa
     2821 agtaggagaa gggagatgga ttactgctgc atgtcccaat cggcgggccg gccctccggc
     2881 acaaacgcgc catcggcccg catgaagcct ctctgcggcg tggcgttctg cgatggcgag
     2941 acgggttcgg gcggcagagc atcggacgag agcgcctgat acgagtgtgc gtaagaatgc
     3001 ggcggcgaat agggcgccgg cggcgtgtag tcagactgcg gcaaagcggc gtcgagcgag
     3061 cccggccccg tgtgcagtgc gaccggctgg tcagcaccac tgcctgcttg cgagacatcc
     3121 atctcttcgt caaccggcgg cggctcgggc aagccatcgg gcgcgtgcgt ctgcggccag
     3181 acatcaggat gcggcttgca gacgcggcgc ggcccggtcg acatattgcc gcgcatcgcg
     3241 agatcgagat gaaagtgatt gtaatgcagg aagttgtatc ccggcccgag gacggtggtg
     3301 aaatcaccgc aggcgccgtt gtcgacatcg cgcagaaagg cttgtgcttg cggatcgccc
     3361 atcgtccaat catgcacgat caaaatattg cgaccatcct gcagccggaa gccgcgcacg
     3421 tcgatcgcat tgccgaaggc atgttcggat agccgcgcgc cgaattcgcc gttgataccg
     3481 cggcagctat aggtgccgaa ggtttcgatc tcgacgacgc gttcgttgta acgagtctgg
     3541 gcggcgggct cgaccacatc cctgacccaa gcgttcagcg ccgcaatcat cgggcaatcg
     3601 aggatataat cgccgtcgag catcacattg ccgtcaagca gggctgccac tttgaacgga
     3661 tgtgtaagac cacaaatgcc cggcccgtcg attgactcga ccgggcggac ataggccgac
     3721 ggccgaacca gattttctgc cagacaggcg ttctccgcct cggcgcgcca agccggtcgc
     3781 tgtgcttgat aattcgagca gccagcgaga tcgaggaaaa gcgccgccac agcggcgaaa
     3841 ggcagaaggc gacggcacat tgcctcaaat tgtgcgaaat atgttaagtg ctcttaaaat
     3901 aacgcggtta acgtgcggct ccggtcgcta gcggccagaa tatgcaacaa cgaatggtct
     3961 ttccgatgga cgacacaccc gccacttcgt ttccgcgcgc gtctggcgcg caaaccatgc
     4021 ccgggctcgt ctgggctttt cgtatgcacg ccgacggaac gccgcaatcg ctgccggtcg
     4081 ataagccgat cgatataaat gacgatggct ggctctggct gcatttcaac ctcgccgatg
     4141 cgcgcgcctg cgcgctgctg ccgcggttcg aggatatccc gccggaagcc gcggttgccc
     4201 tcaccgcgcc cgacggtgcc cagcagctgc atgtcgaaga cacctgcgtc tacggaattt
     4261 ttgccgacct gatcgccgat ctcgacggag ctacgaatca ttttggctat ctgcatttcg
     4321 cgatgacgga caggctgctg gtgagtgcgc ggcgccgcgc gatgaacgca gccgaatccg
     4381 cgcgtcaagc gcttgaccgt ggccacaagc tgcatcgcgt ctcgtctcta tttgaattga
     4441 tcgttaacca cgtcgccgac tccatcgaca aatattccga tcagctcgat gaagatatgg
     4501 acgatatcga ggacgagctt tggcagcgcg ccgcaaccga ccagcgcgcc aagctcgcga
     4561 aagtccgtaa gggcgcggtg catttgcatc gccagatgca agggctgctc gccctgttcg
     4621 aacgcgttgg ccacaggctc gacaccgcgc cgccacccga gctgcgacta aagacgagca
     4681 ttctgatgca gcggctcgaa aacctcgatc gcgatatcat cgccatgcgc gaacgtgcgc
     4741 atatgctgca ggaggaagtt gcgctcaaga tcgccgagga taccaaccgc agcctttatc
     4801 tgctgacgat catcacgacg tttttcctgc ccgcgagtct cgtcgccggc attttcggca
     4861 tgaatgtgaa gaacctgccc ttcaccgaca tttccagcgg cttcgtctgg tcgatggtga
     4921 ttctggtcgg cgcatcgctc gccattctca tcttcatgca gcgcatgcgc attcttttgc
     4981 gctagaactt ggcgaggagc cgccacgaat cgacatggcc agaattgcga tcgccaaaaa
     5041 cctgtcgctc gacgaaaacg agatcgagtt tacttttctg cgcgcctccg gcccaggcgg
     5101 acagaacgtc aacaaggtcg aaacggcggt gcaattgcgc tttgatgtgc gcaattcgcc
     5161 ctcacttgac gattatgtga agcgccggct ggaaaagcta gccggcagca ggctgacact
     5221 ggacggcatc ctgatcattt ccgcacagcg atttcgctca caggagcgta accgtgaaga
     5281 tgctttggtc cgcctcggtg cgctgatcgc caaggcctcg gagcgtgtga aacggcgcat
     5341 cgcgacgcgg ccgacgttcg catcccaaca acgccgtcgc gacgccaagc aaaaacgcgg
     5401 agagatcaag aaactgcgtg gcgcgccctc ggattgaggc ggccggctac gtcaagtgga
     5461 ttgccggcgc cgcgcgtgct atggccagcg cgagcgagag ggagggatgc atgcgtgtag
     5521 cttttgtgac ggccacggcg ctggctgcga tctttgcgac ggccgcggcg gccgacgact
     5581 acccgccgcg caaaccgggg ctttgggaaa tcgccgtcca taatcagtcg ctaccggacg
     5641 ttacgatgaa aatgtgcatc gacgccgaga ccgaccagct attccataaa ttcagcggcg
     5701 acatccgttc caaacattgc agcaagagcg gcctcaagac ggtcggcaat gtggtgacgt
     5761 cggaaagcga ttgtacgctc ggcggcacaa aagtggactc cgtcgcggtc gctacattca
     5821 ccggcgattc ggcctaccac gtcgatatca agacgcattt cgatccgccg aagctcggtc
     5881 acagcgacgt tgcgatgagc caggatgcca aatgggtcag cgaatgcccg gcagacatga
     5941 aacccggcga tttcgacatg ggtcacggga tcaagatgaa tatcaaaact gtcgaacgcc
     6001 tacggtcgct actacctggg cacagcgact agtaagccag gccgctgctg ccggttgaac
     6061 gaagcaatcc ggttcatgtc cggattgctt cactataggc acgtctcgca atgaggttca
     6121 gatcaatcag cttgccggtg actccggcgc gccgttttgc ggcggctggt tgttgcgctg
     6181 agacatgaag cgatcaaact cctcgcgatc cttggcgcgg cgcagattct cgacgaagct
     6241 cgtgaattcc tgctcggccg cgcgcagcct ttcgcgctcc gcttcgagac gcgtcagttc
     6301 tgcttcgcgc cattcatcga acgcgagatt accggtcgaa gacggtttcc attgccacgg
     6361 tgggccgcca gcaccacgcc gctcgcctgc gccggcatag gcccaattgt aaaagtcctg
     6421 ccggcgcatg ccgccgaagc cgtgacgacc cattttctgc tcccatttct cgcgaaattt
     6481 ctgctgcatt ttggcgctga cgaaacccgg tagatcgccg gcataggcgc tcttcttctg
     6541 ccagatcttg gcgatcagga tggcgagccc aatcggccag taaagaatga agccgagaac
     6601 catcgcgacg atttcgatcg gccgccaatc atgcagagtc ggaagaccca tcgaagcgcc
     6661 gtttggcgcg cggtcaaagc tgcgtgtcac acacataagc tcctccatgc agcatcagtg
     6721 ctgcaatgta aaagtatgtt acattaacat tcaaagcctg tcgaggcggc gtccgttcaa
     6781 atcgcgtaac atgctgcttg cgaagctatt tccgatgaag cgccgtggcg atcaacgctt
     6841 ccgcgccccg gtcgaggatc gccgcgggat cgtcaccgcg ggccgagtcg aaatggccgg
     6901 caagggtcag catcgcggtg ccatgtgcca gcgcccagat ttcgagcgcg aggcggcgga
     6961 tgtcggcggg atcgcccccc cccgtcagga ttacgcctgc ggcggcccgc gtcagaccgt
     7021 cgaacgcttt gttggcgacg aaatgcaccg cccgcccttg caggctatgg gcgttgtcga
     7081 acatcgcggt gtaaaggccg ggttccgcgg tcgcaaaatt cagataggcg cggcccatgc
     7141 gcttcagcgc cacacgcggt tccgggcgtc cctcatccca ggcgccagcg agccgctcgc
     7201 cgaaaaggtc gaagccgcgg cgggccagtt cgcccatgag atcctgccga tcggcgaaat
     7261 gacggtaggg cgccgcaccc gtgacgccga cgcgcttggc ggcttccgcc aaggtgaagc
     7321 cgcccggccc atgttcagcc atcagcgccc gcgccgcctc gaccaaagcc tccttcaggc
     7381 cgccatggtg atagccgcgg cgctccggcc cgaaatggcc gccatgtttc cagtttcgtt
     7441 tcatatgaat catagggtca gcttagatgc gcgcacgccg aagtgccaat tggccgcctc
     7501 gccgtttgcc ggtataaggc cgcatgcttt cctttatccg acttcttttc gtttgcatca
     7561 tttttgccgc accggctttc gcggccgagc aggcttcgcc ggtgccgttc caaaacaaga
     7621 gtgcgccgac gaaagtgccg tccgacgtat ccgtgctcgc ttatggcgcg gcgcatcccg
     7681 aatgtctcga atggacggat agctgccgca cctgcatgcg cagccccgac ggtcgtgtcg
     7741 gttgctcgac acccggcatc gcctgccagc caaaagagat cgtgtgcaag aaagaaagta
     7801 aatgagcgcg ctgcgtgcaa gctgacacgt cgcgaacgga aagcgcggca cgactttggc
     7861 cgccgcttgc gatttttatc gtgcgcgaaa agagactttc ggcttgggtc gcggcgcgcg
     7921 gacccttcat tcgtttttgt tacgaatttc tgcgtttcgg cctgaaacag ggatgggctt
     7981 gcctcttcgg cggcgcgatg gtcgcgctac tgaccggcac acatcttttc tatcccaccc
     8041 atgccccgct cgcgcgttac gattttttag ttgtcgcggc gattgttcta caggccgtaa
     8101 tgctggcaag ccggcttgaa actttcgacg agttcaaagt catcctcctc tttcacgccg
     8161 tcggcaccgg aatggagttg ttcaagacag cgacgggctc ttggctcttt cccgaacccg
     8221 gcttcttacg gatcgctgcc gtgccgctgt tcacgggatt catgtatggc acgatcggca
     8281 gctatatcgc acgagcatgg cgcctgttcg attttcactt tcgtcatcat cccccgcttt
     8341 gggcaacgaa cgtcctggcg attgcgatct acctgaactt cttcgccgat cattacggca
     8401 tcgattttcg ctacgcgctt ttcgccggcg cgatcgtgct ttttgcgcgc acgaaagtct
     8461 tcttcaaggt ctggagaacc tatcgcgcga tgccacttct gctcggcttc ttccttgtcg
     8521 cgctgttcat ctggtttgcc gaaaatgccg gaacggcggc ccacgcatgg ctttatccga
     8581 accagcgtgc cggttggtcg cttgtcgcga tctggaagct cggctcctgg ttcctgctga
     8641 tgatcatttc ttacatcatg gtcgcctgga tcaaccgtcc tcgcgcattc gcacccgaat
     8701 tggacggacg gatcgtcgat agcaatttcg tagattcgta caagggcctt tcggccgacg
     8761 agacttcgaa ttgaacctgc ggcctcacgc cgcctgaacg aaactttcga gaaccatctt
     8821 gcggccagcc ttgtcgaaat cgaccgtgag cttgttgcca tcgacggccg cgacggtgcc
     8881 cggcccgaat ttcatgtgaa acacgcgcgc gcccttgttg aaggatgagg cggcggaaga
     8941 tttggcgacc aattcgccgt caatcgtcat cggcccgcgg cgtgcggcgc tatcgcgcga
     9001 aaagcccggc tgctgcgtct cctcggcatt gcggcgctgc gcgcgctgcc agcccggcgt
     9061 cgcgtaagac gagcgatagg tatccatatt ggcgaagcgc gattgcgcat aattgccgta
     9121 gctgcggcct gacgccgaat cgacgacctc gacatgcgcc tccggcaatt cgtcgagaaa
     9181 gcgcgagggg atcgtcgtct gccacagacc ccggatgcgc cggttggtgg caaaatagat
     9241 tttcgcc
//
LOCUS       DASWOG010000325         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_99231,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000325 DASWOG010000000
VERSION     DASWOG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99231"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGB93_06765"
     CDS             complement(<1..338)
                     /locus_tag="VGB93_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145840.1"
                     /translation="MNAPATAIARPSPRLPVKTALTTPVRAAGTLMLSLHHDLAALRD
                     SWVAFEETADGTVFQTYEWLAAYHNFIGAPRGEIPCVAIARAGDGTIACLLPCAVRRG
                     GFLRDLTWLD"
     gene            complement(416..1675)
                     /locus_tag="VGB93_06770"
     CDS             complement(416..1675)
                     /locus_tag="VGB93_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0145841.1"
                     /translation="MEEVQTLIRPDTLVDSERCAPALSTDVVIIGGGLAGSLAGAMLG
                     RAGIDCVVVDPHTEYPPDFRCEKLDGDQVARLQKTGLADEVLSAATFDGEAWVARMGV
                     VVDKRPGDQHGIFYASLVNRMRSSIPSKVRFCYATARRLTTDTDRQSVTLSDGTTISA
                     RLAILSNGLGLALRDELGLTREYISRSHSVTLGFNMTAVGRRHFDFRALTSYAKSPSD
                     RTALITIFPIGSTVRANLFTYRTMDDPWLSEMRHAPEKTLRALMPELEQMIGPFSIEK
                     PIQIRPVDLYVSRGHFQPGLVLVGDAFATSCPASGNGVRKVITDVERLCNVYIPQWLA
                     TPGMDTEKIAAFYADPVKCACDRSSLNKALTLKRYSTDAAPIWRARRNARFVQHLALG
                     FARRLWMRLSGQFSSGADHAVLPVRSS"
     gene            complement(1654..2805)
                     /locus_tag="VGB93_06775"
     CDS             complement(1654..2805)
                     /locus_tag="VGB93_06775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145842.1"
                     /translation="MGIVVERAGAERLASIEPAWRDLVGRALEPNVFMDPRLLAAAIA
                     SVPKRKFAILLAWDSNRADARLLGLWGGVIARALLPLCVLRIPAGPHGYLATPILDSG
                     MAEAVLHAMLDAIAAASDLPNILSATPMRADGATMPILHRVMRARQAGLHVFARWRRP
                     LLQSDMGGNAYLAAAKSSSSRKKLRQHRRRLAERGALTLHAYLSPADVSAALERFLIL
                     EAAGWKGRHGTALNSSAADVAFARRMITTLSARGDVRIYALHLDNSPVSMQIMLRCGD
                     AAFAWKTTYDEDMHEVSPGMLLLEDSTTALLADPAIAFADSCAFDENSYMAVWRERET
                     LAELWIDARCGGSLVFNTCARIQGILLRMRSLLKRLYGMGMETWKKFKP"
     gene            complement(3109..>3431)
                     /locus_tag="VGB93_06780"
     CDS             complement(3109..>3431)
                     /locus_tag="VGB93_06780"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY0145843.1"
                     /translation="FVTGYPVMAILICGHLVRASIGPAERILSMLGQQRVCVFVYAAA
                     LVFNIAASVTLVPRYGGVGAATATASAFMVETALLFWLANKRLGLHLFTWHSRSRDGF
                     RKQN"
BASE COUNT          761 a          993 c         1055 g          622 t
ORIGIN      
        1 tcgtcaagcc aggtgaggtc gcgcaggaag ccgccgcgtc ggacggcgca cggcaaaaga
       61 caagcgatcg tgccgtcgcc ggcgcgggcg atggcaacgc aggggatttc gccgcgtggc
      121 gcaccaatga aattgtgata ggcagcaagc cattcataag tctggaagac ggtgccgtct
      181 gctgtttcct caaaagcgac ccagctgtcg cgtagcgcag ccagatcgtg gtgtaagctc
      241 aacatcagcg tccccgcagc ccgcaccggt gtcgtgagcg cggtcttaac aggtaaacga
      301 ggcgatggcc tagcgatggc cgttgctggg gcgttcattc ttcactccct acgccgagaa
      361 cctcggccgt gcagcaagtc cggcgctgaa attaaaaata attggacggc ggcttttaac
      421 tgctccgaac cggcaggacc gcgtgatcgg cgccgcttga aaactgtccc gaaagccgca
      481 tccacaggcg acgagcgaag ccgagggcga gatgttggac aaagcgagca tttcgccgcg
      541 cacgccagat cggtgctgcg tctgtcgagt agcgtttgag agtcaatgcc ttattgagcg
      601 aagaccgatc gcaggcgcat ttaacaggat cggcataaaa ggccgcgatc ttttcggtgt
      661 ccatgccggg cgtcgccagc cattgcggga tatagacatt acagaggcgt tcgacgtcgg
      721 taatgacctt gcggacaccg ttgccgcttg ccggacagga ggtggcgaag gcatcgccaa
      781 cgagcacaag accgggctgg aaatggcccc ggctgacata gagatcgacc gggcgaatct
      841 gtattggttt ctcgatgctg aagggtccga tcatctgctc aagttctggc atcagcgcgc
      901 gcaacgtctt ctcaggagcg tgacgcattt ccgacagcca ggggtcatcc attgtgcgat
      961 aggtgaagag attggcacgc acggtgctcc caatcgggaa aatggtgatc agcgccgtcc
     1021 ggtcggaggg cgatttagcg taggaggtca acgcgcggaa atcgaaatga cggcggccga
     1081 ccgccgtcat attgaagccg agcgtgaccg aatgggagcg gctgatatat tcgcgcgtca
     1141 aaccaagctc atcgcgtaag gcaagaccaa gaccattcga caatatggca aggcgcgccg
     1201 aaatcgtcgt gccgtcggaa agcgtcacgc tctgacgatc ggtatcagtt gtcagccgcc
     1261 tcgccgtcgc atagcagaag cggaccttcg acgggatcga cgaccgcatg cgattgacca
     1321 gcgaggcata gaaaataccg tgctgatcgc ccgggcgctt gtccacaacg acgcccatgc
     1381 gtgccaccca cgcttcgcca tcaaacgtcg cggccgacag aacttcgtcg gcaaggccgg
     1441 tcttttgtaa ccgcgcgact tgatcgccat caagcttttc gcagcggaag tcgggcggat
     1501 attcggtatg cggatcgacg acgacgcaat caatgccggc gcgtccgagc atcgcacccg
     1561 ccaatgaacc tgcgaggccg ccgccgatga tcacgacatc ggtcgaaagc gcgggcgcgc
     1621 accgctcgga atcgaccagc gtatcgggcc tgatcagggt ttgaacttct tccacgtttc
     1681 catccccatc ccatataatc gtttcagaag gctgcgcatt cgcagcaaga tgccttgaat
     1741 ccgtgcgcaa gtattgaaga ccagtgaacc gccgcaacgc gcatcgatcc agagctcggc
     1801 aagcgtttcg cgctcgcgcc agacggccat gtagctattt tcatcgaatg cgcaggaatc
     1861 ggcgaaggca atggctggat cggccagaag ggctgtcgtg ctgtcttcga gtaacagcat
     1921 gcccggcgag acctcgtgca tgtcttcatc gtaagtggtc ttccaagcga aggccgcatc
     1981 gccacagcga agcattattt gcatgctgac agggctgtta tcgagatgaa gcgcatagat
     2041 tcggacatcg ccgcgggctg acagcgttgt gatcatccgg cgcgcaaatg cgacatcggc
     2101 ggccgaactg ttcagcgcgg tgccgtggcg gcccttccat ccggcggcct ccagtatgag
     2161 aaaccgctcg agggcagcgg agacatcagc cggtgagaga taggcgtgca gtgtaagcgc
     2221 gccacgttca gccagacggc gacggtgctg acgcaacttc ttacggctgg aggacgactt
     2281 cgcagctgcc agataggcgt tgccgcccat gtcggattgc aaaagtggcc gccgccatcg
     2341 cgcgaagaca tgcaggcccg cctggcgagc cctcatcacg cggtgcagga tgggcatggt
     2401 cgccccgtcg gcccgcattg gcgtcgccga tagaatatta ggcaaatccg aggccgccgc
     2461 gatggcatca agcatggcgt gaagcaccgc ttcggccatg ccgctgtcca gaatcggcgt
     2521 ggcgagatag ccatgtggcc cggcgggtat tctcagcacg cacagtggca gcagagctcg
     2581 ggcgattact ccgccccaca ggccaagaag gcgcgcatct gcccgattgc tgtcccaagc
     2641 gagaagtatc gcgaacttgc gttttgggac ggacgcaata gcagcagcca ataggcgcgg
     2701 gtccatgaac acattgggct cgagtgcacg accgacgaga tcgcgccaag ctggctcgat
     2761 cgaggctagg cgttcggcgc cagcacgctc gacgacaatg cccatcgaga catccgcttc
     2821 gcgcgtggcg gagggacgtt catccggcat cgacgacatg gcttcaaagt caaatactct
     2881 aaaagcttgg aacgcttcgt acaccgcact ccccagatat gatccgtccg cgtgcgggat
     2941 tgtagcgccg ggcacttcac ggggtcttaa gcgatgcgct ggatttcgaa gttgagatta
     3001 acgaaggttt agggagatga cttaaatggt ggcctgcgaa gcggtgtcac aaaatgtaag
     3061 ttctcttcgt agcgatcgcg ctcacgcaga ttgccaagac ttattcacct agttctgctt
     3121 tcgaaatcca tcgcgtgagc gcgagtgcca tgtgaataaa tgcaggccga ggcgcttgtt
     3181 cgcgagccag aatagcaacg cggtttcgac catgaaggcg ctcgccgtag ccgtggctgc
     3241 gccgacgccg ccataccttg ggacgagggt cacgctcgcc gcaatattaa agaccaaggc
     3301 cgccgcatag acaaatacgc agacccgctg ctgtcccaac atgctgagaa tacgctcggc
     3361 gggcccgatg gaggcgcgca cgagatgacc gcagatgaga atagccatca ccggatagcc
     3421 ggtaacgaag t
//
LOCUS       DASWOG010000326         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319247,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000326 DASWOG010000000
VERSION     DASWOG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319247"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VGB93_06785"
     CDS             complement(<1..553)
                     /locus_tag="VGB93_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEY0145844.1"
                     /translation="MSLASRVWNLVRRYPALLVLALLLLEVAPARAIESVRIPLDATA
                     IDLTKAVERYGAQGERLLVSTAPGSDGIVRRIEVRALEPGSHPNWIVFALTNDTDEQL
                     ERLVVAPHFRLVGSGVIWPDLGASRISAITASQSFPPERIESADADVFRLTIDPGTTV
                     TYVAELRSAGLPQLYLWQPDAYKD"
     gene            complement(564..1178)
                     /locus_tag="VGB93_06790"
     CDS             complement(564..1178)
                     /locus_tag="VGB93_06790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0145845.1"
                     /translation="MALFRLGAPSEISSFVRGEGVFLRPPEPRDYEAWSDLREKSRAF
                     LTPWEPTWPGDDLTRTSFRRRLRRNLQEMSNDEAYPFFIFRDDDDRLVGGLTLGQVKR
                     GVAQSATLGYWMGMPYAGKGFMSRAVRAMTGYAFTSLRLHRIEAACLPHNAVSIQLLE
                     RVGFKREGLARAYLRINGLWQDHLLYALLETDPLPLRVLRRDRG"
     gene            complement(1190..2452)
                     /locus_tag="VGB93_06795"
     CDS             complement(1190..2452)
                     /locus_tag="VGB93_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0145846.1"
                     /translation="MSVEITTLPSGLRIVTDRMPHLETVSLGVWVAAGSRHERPGERG
                     LSHLLEHMAFKGTRRRSARDIAEEIESAGGDLNAATGVEQTAYYAHMLASDAGLAIDI
                     LADILTDSVFDKDELEREKEVILQEIGAVEDTPDDLVFDLFNAAAFPEQPIGWPILGT
                     PEHVKAFDRSMISNYLAKHYRSGVTTIGAAGALDHAQIVDAAASRFANMAAQVGEAVT
                     PARYLGGDTRLSRRLEQAHVVIGFEGLSYAAPGYYALQVFTNAVGGGMSSRLFQEVRE
                     NRGLAYTIYSFHWGYADTGLFGFYGATSKAQVRELVPVALECLAKAAEDLSEAEIDRA
                     KAQMNVSQLTALESSTARSEQIARQILAYGRVLSRDEILARIDNLTVNEIRAAGAAAL
                     RTVPTVAAVGPVAKVHTPDRVRELVGRA"
     gene            complement(2449..3873)
                     /gene="thrC"
                     /locus_tag="VGB93_06800"
     CDS             complement(2449..3873)
                     /gene="thrC"
                     /locus_tag="VGB93_06800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0145847.1"
                     /translation="MNYISTRGAAPVLSFTDALIAGLAEDGGLYLPDSYPHFSAAEIA
                     AFAGQPYAAVAESVILPFVSGTLERSVLRDMIASAYTGFDHVAVAPLSQLGDNLFLLE
                     LFHGPTLAFKDLAMQLLGRLMQHVLAARGQRATIIGATSGDTGAAAIAAFQGLPEVDV
                     FILYPNGRVSDVQRRQMTTIAADNVHTIAIEGTFDDAQNMVKALFAHKRFRQEFALAG
                     VNSINWARVMAQIVYYFTSAVALGGPHRPVCFVVPTGNFGDILAGWIAKKMGLPIERL
                     VIATNSNDILVRTLNTGRYETGRVEATLSPSMDIQVSSNFERLLFEAYGRDASAVGGL
                     MNSLQQSGGFSVAAAPLAAIRAEFDAARVGETETSEEIARVWRETQRLIDPHTAVGVG
                     AARAALTAHPNVPAIVLGTAHPAKFPAAIEGATGQHPPLPAHLADLETRPEYFDVLPN
                     DQHAIENFIGERLQGSAASRRAAS"
BASE COUNT          808 a         1214 c         1233 g          683 t
ORIGIN      
        1 tgtctttgta ggcgtccggt tgccaaagat agagctgcgg cagaccggcg gagcgcagct
       61 ctgcgacata ggtcaccgtg gtgcccggat cgattgtcag gcggaagaca tcggcatccg
      121 cactttcgat ccgctccggc ggaaagcttt ggctcgcggt gatcgccgaa atgcgcgaag
      181 caccgaggtc aggccagatc acgcccgagc cgacgagccg gaaatgtggc gcaacgacaa
      241 gccgttcaag ctgctcgtcc gtatcattgg tcagcgcgaa aacgatccag ttgggatgcg
      301 agccgggttc gagcgcgcgg acctcgatgc gccggacaat gccgtcggag ccgggcgcgg
      361 tcgagaccaa cagacgttca ccctgagcgc catagcgttc aacagctttc gtgagatcaa
      421 tcgctgtcgc gtcgagcgga atgcgcacgg attcgatggc gcgagccggc gcgacctcca
      481 acaacagaag cgcaagtacg agcaaggccg ggtatcggcg caccaaattc cacacgcgtg
      541 aagccagcga catttttctc cactcagcca cggtcgcgcc gcagcactcg caagggcagc
      601 gggtcggttt cgaggagcgc ataaaggaga tggtcttgcc aaagcccgtt gatgcgcaga
      661 taggcgcgtg ccagcccttc ccgcttgaag ccgacacgtt ccagaagttg aatcgatacc
      721 gcgttatgcg gcaggcaggc cgcctcaatc cgatgcaagc gcagagacgt gaaggcatag
      781 cctgtcatgg ccctcaccgc ccgcgacatg aatcccttgc ccgcgtaggg catccccatc
      841 caatagccaa gtgttgcaga ttgcgcgacg ccgcgcttca cctggccaag cgtcagaccg
      901 ccgacaagcc ggtcatcgtc atcgcgaaag atgaagaacg gataggcttc atcgttcgac
      961 atttcctgca aattgcggcg cagcctgcgg cggaaagatg tgcgggtcag atcatcgccg
     1021 ggccatgttg gctcccaggg tgtcaggaag gcacggctct tttcacgcag atcggaccag
     1081 gcctcatagt cgcgcggctc cggcggacgc agaaagacgc cctcgccgcg gacgaaactg
     1141 ctgatctcgc tcggcgctcc gagccggaac agggccaacc gatcctccct caagcacggc
     1201 cgacgagttc gcggacgcga tccggcgtat ggaccttggc cacgggcccg acggcagcca
     1261 ccgtcggcac cgtgcgtaag gccgctgcgc ccgctgcccg gatctcattc acagtcagat
     1321 tatcgattct cgccagaatt tcatctcggc tcagcacccg gccataagcc aggatctggc
     1381 gcgcgatctg ctccgagcgg gcggtcgagg attcaagcgc cgtaagctga gagacgttca
     1441 tctgggcctt cgcgcggtcg atttcagctt cgctcaaatc ttccgccgcc ttcgccaaac
     1501 attcaagggc cacgggcacg agttcgcgga cttgcgcctt gcttgtcgcg ccatagaagc
     1561 cgaagaggcc ggtatcggca tagccccaat gaaaggaata gatcgtatag gcaagcccgc
     1621 gattctcgcg aacctcttgg aaaagccgcg aagacatgcc gccgccgacc gcattggtga
     1681 aaacctgcag cgcataataa ccaggcgccg cgtaagacag gccttcaaag ccgatcacga
     1741 catgcgcctg ttcgagacga cggctcaagc gcgtgtcgcc accgagataa cgcgccggcg
     1801 tcaccgcctc cccgacctgc gccgccatat tggcaaagcg cgacgcggcg gcgtcaacaa
     1861 tttgcgcatg atccagtgcg cccgccgcgc cgatcgtcgt gacaccactg cgataatgtt
     1921 tggcgagata gttcgagatc atcgaacggt cgaacgcttt gacatgttcc ggcgtaccga
     1981 gaatgggcca gccaatcggc tgctcgggaa aggccgcggc gttgaagagg tcgaacacga
     2041 gatcgtcggg cgtgtcctcg accgcgccga tctcctgaag gatgacctct ttttcgcgtt
     2101 cgagctcatc cttgtcgaag acgctgtcgg tcagaatgtc cgcgagaata tcgatggcga
     2161 gcccggcgtc gctcgccagc atgtgagcat aataagctgt ctgctccaca cccgttgcgg
     2221 cgttgagatc accgccggcg ctttcaatct cttcggcaat gtcgcgggct gagcgacgcc
     2281 gcgtgccttt gaacgccata tgttcgagca gatgcgaaag accgcgctcg cccggccgct
     2341 catgccgcga gcctgccgca acccagacgc cgagcgatac ggtttcgaga tgcggcatcc
     2401 ggtcggtgac gatgcgcagg ccagacggca aggtcgtgat ttcgacgctc atgacgccgc
     2461 ccttcgcgat gcggcgctgc cttgcaaacg ttcgccgata aaattttcaa tcgcgtgctg
     2521 atcgttcggc aggacgtcga aatattcggg ccgcgtttcg agatcggcga gatgcgccgg
     2581 cagcggcggg tgctggccgg tcgcgccttc gatggcggcg gggaatttcg ccggatgcgc
     2641 cgtgccgagg acgattgcag gcacattggg atgcgcggtc aaggctgcgc gcgcggcgcc
     2701 gacgccgaca gcggtgtgcg gatcaatcaa tctttgcgtc tcgcgccaga cacgcgcgat
     2761 ctcctcactt gtttcagtct caccgacccg ggcggcatcg aattcggcgc ggatcgctgc
     2821 gagtggcgcg gcggcgaccg aaaagccgcc ggattgctgc agcgaattca tcagtccgcc
     2881 gacagccgag gcatcacggc cataggcctc gaacagcagc cgctcgaaat tcgaggacac
     2941 ctgaatgtcc attgacggcg acaatgtggc ttcgacgcga ccggtctcgt agcggcccgt
     3001 gttcaacgtg cgcacgagaa tgtcattgga attggtcgcg atgacgagcc gttcgatcgg
     3061 cagccccatt tttttcgcga tccagcccgc gaggatatcg ccgaaattgc cggtcgggac
     3121 gacgaagcaa acgggccggt gcgggccccc aagcgccacg gcgctcgtga aataatagac
     3181 gatctgcgcc atgacgcgcg cccaattgat cgaattgaca cctgcgagcg caaactcttg
     3241 acgaaagcgt ttgtgcgcga agagcgcctt caccatgttt tgcgcatcgt cgaacgtgcc
     3301 ttcgatggcg atcgtatgaa cattatcggc ggcaatcgtc gtcatctgcc ggcgctgcac
     3361 gtcggagacg cggccattcg gatagaggat aaaaacgtcg acctcgggca agccctgaaa
     3421 ggcggcgatg gcggcggcgc cggtgtcgcc cgacgtagcg ccgatgatgg tcgcgcgctg
     3481 gccccgcgcc gccaggacgt gctgcatgag ccggccaagg agctgcatcg caaggtcctt
     3541 gaacgccaaa gtcgggccgt gaaacaattc gagcaggaaa agattgtcgc ctagctgcga
     3601 gagcggcgcg accgcgacat gatcgaaccc ggtataggcc gacgcgatca tgtcgcgcag
     3661 cactgagcgt tcgagcgttc cggagacgaa tggcaagatg acggattccg ccacagcagc
     3721 gtaaggctgg cccgcgaaag cggcgatctc ggccgcagaa aagtgaggat aagagtccgg
     3781 cagatagagg ccgccgtcct cagcgaggcc ggcgatcagc gcatcggtaa aggaaagcac
     3841 gggcgcagcg ccgcgcgtcg agatgtaatt cactttccgg cctaggttgt cacgattccg
     3901 gggtggttta gcacaaaacc gcggctgcgg ggattttt
//
LOCUS       DASWOG010000327         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_135841,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000327 DASWOG010000000
VERSION     DASWOG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135841"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="VGB93_06805"
     CDS             <1..2129
                     /locus_tag="VGB93_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY0145848.1"
                     /translation="LAGPENRSDGLKIRVLRASANGASQGLVRALDPKGLAIGETRFE
                     FPKTSLETKASFDLPVDLRNDIARLEILDERSAGAVSLLDARWKRRTVAIVSDETADV
                     SLPLLAPNYYLHKALAPFADVREANPGTPDPIGVLLDQNPAVMILADVGTLSDTDYAR
                     LKQFVDDGGLLLRFAGTRLAAGSDGLVPVRLRRGGRVLGGALSWETPKRLAPFDTQSP
                     FLGLATPKDVSVRRQVLAEPDADVPGKTWAHLVDGTPLVTAAHQGKGMIVLFHVTADT
                     TWSNLPISGLFVDMLRKIVDLSTLLTKPDATNAGNEKVESVPPTSILDGFGVLGAPPQ
                     TAKPVPINFEGAATADHPPGFYGPPDQLLAVNALRATETLQTADYSGLGFAQEALRQA
                     VPTDLRPILLAIAFLLFIADSLASLWLSGGLSRRISRAAAAIIIIGMAASLVPVAHLR
                     AAPRADTPLSQSDLQSALNTRLAYVISGDPDVDGESKAGLTSLSQVLAQRTSLIPGTP
                     VGVDPARDELAFYPMLYWPIVADRPQPSAAAIAKVAAYMKQGGTIIFDTRDALTAHAD
                     GPPTPETLWLRQPLQGVDVPQLEPIPPDHVVTKTFYLIDGFVGRYETGQTWIEALPPL
                     NPADGQRPARAGDGVSPILITANDLAAGWAADDNGDNLYALMPGGPRQHELALRGGVN
                     LVMYTLTGNYKTDQVHVRDLLERLAH"
     gene            complement(2146..3099)
                     /locus_tag="VGB93_06810"
     CDS             complement(2146..3099)
                     /locus_tag="VGB93_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY0145849.1"
                     /translation="MREIAFVVYPGYSVMALAVVTAFEVANTIADDPPYGLHFVSETG
                     GRVKTSSGMMIESEAFGEKAFDTLVVGGATIPASSTPGLIAYVKDAPKRHRRIAAVCT
                     GAFVLAEAGLLDGRHATTHWLHARELQRRFPKVKMDEDRIFINDGPIWTSAGMTAGID
                     LGLALIESDLGPELAKSVARKLVIYHRRVGGQSQFSTLLELSPKSDRIQMALTYARDN
                     LHKPLTVPELADVAHLSPRQFSRAFHAETGQSPAKAIENLRVEAARNLTEESRHPIDI
                     VARQTGFSNRDHMRRAFLRAFGEPPQVLRRNARHEAIDYRG"
     gene            3217..4035
                     /locus_tag="VGB93_06815"
     CDS             3217..4035
                     /locus_tag="VGB93_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145850.1"
                     /translation="MSSPSPKGTALITGASSGIGAVYADRLAKRGYDLILVARNEQRL
                     NAVASRLRGETGRQVTVLAADLNDKASLAKVEAVLRDDPSIIMLVNNAGVGSVAPILQ
                     ADVDKMEAMIDLNITALTRLSYAAAPAFVARGAGTIINIASVVGIALEALNGVYSASK
                     SYVLSFGHSLQKDLAEKGVRVQTVLPGATATEFWDIAGYPPQKHSEITMSAEDCVDAA
                     LAGLDAGELVTIPGLHEGELWTRWEAERRGLSMKFGNAKPAPRYMAGVDKVGVA"
     gene            complement(4037..5362)
                     /gene="fliI"
                     /locus_tag="VGB93_06820"
     CDS             complement(4037..5362)
                     /gene="fliI"
                     /locus_tag="VGB93_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEY0145851.1"
                     /translation="MTALARLEQAINYGLEEIGDLRIGGFVSEVAPSHYRITGLSQFL
                     KLGECVGMEHDGETQLGEVVRVDVSGATVKSFDVGAAAGLGDSAYRIGPLHLSPDVSW
                     KGRVIDALGRPLDGQGALRRGEHTRSIESPPPQALNRARVKTSLRTGVRAIDLFMPLC
                     AGQRIGIFAGSGVGKSTLLGMLAGAKGFSAVVVGLVGERGREVREFLDDSLGQNRKLA
                     VTVVSTGDESPMMRRLAPKTAMTIAEFFRDRGENVLLILDSVTRFAHAARDVALAAGE
                     PAVARGYAPSVFSDLPKLLERAGPGVEGSGSITGIFSVLVDGDDHNDPVADNILGTLD
                     GHIVLDRAIADLGRFPAVNILSSISRLAQHVWTKEQQTLILKLKSMVARYEDTRDLRL
                     MGGYQAGQDTDLDQIVALVPRLYDALTQSPSSSPSRDAFRELSDALQAK"
     gene            complement(5385..>6053)
                     /gene="flgF"
                     /locus_tag="VGB93_06825"
     CDS             complement(5385..>6053)
                     /gene="flgF"
                     /locus_tag="VGB93_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEY0145852.1"
                     /translation="ESLALNVANMNTVGYRAAGVSFHTYVSRAGADPVDYATAGKDYV
                     TRTEGPLERTDNPLDVAVQGEGFLAIQTPAGTVYTRDGRMRMKASGELESLAGHPILD
                     AGGTPLILDPNAGPPVIGGDGMITQGINQVGALGLFQIDPNARFSRYENSGVIPDRPA
                     SPILDFNTNGIVQGHIEGSNVNPIMEMAKMMTIQRAFESVTGGSEKNESTLKDALPIL
                     GGTS"
BASE COUNT         1154 a         1858 c         1876 g         1165 t
ORIGIN      
        1 cgctggccgg ccccgagaat cggagcgatg gcctgaaaat ccgcgtcttg cgcgcctcgg
       61 ccaatggcgc atcgcagggc cttgtccgcg cgcttgatcc aaaaggcctt gctatcggcg
      121 agacgcgttt tgaattcccc aagacgtcct tggaaaccaa agccagcttc gatctgccgg
      181 tcgatctacg gaacgatatt gcccggttgg aaatcctcga tgagcgctct gccggcgcag
      241 tgtccttgct cgacgcgcgg tggaagagac gcaccgtggc catcgtcagt gacgagacgg
      301 cggacgtctc gctgccgctg ctggcgccga attattatct gcataaagcg ctggcgccct
      361 ttgccgacgt gcgcgaggca aatcccggga caccggatcc gattggcgtc ctgctcgatc
      421 agaatccggc ggtgatgatt cttgccgacg tcggcacgct ctccgacacg gattacgcgc
      481 ggttgaagca attcgtcgat gacggcggcc tgcttttgcg tttcgcgggc acgcggctcg
      541 cggcaggctc cgacgggctg gtgccagtgc ggctgcggcg cggcggccgc gtgctgggtg
      601 gcgctctatc ttgggaaacg ccgaagcgac tcgcgccatt cgatacgcaa agtccattcc
      661 tcgggctggc gacgccaaaa gacgtgagcg tgcgccgtca ggtactcgcc gagcccgatg
      721 ccgacgtgcc gggaaagaca tgggcgcatc tcgtcgatgg cacgccgctt gtcaccgccg
      781 cgcaccaagg caaaggcatg atcgtgctgt tccatgtcac ggcggatacg acgtggtcga
      841 acctgccgat ctccggcctg ttcgtggata tgttgcgcaa gatcgtcgat ctctcgacct
      901 tgcttacgaa accggacgca accaatgcgg gtaatgagaa ggtggaaagc gtgccgccga
      961 cgagcattct cgacggattc ggcgtccttg gggcgccgcc gcagaccgca aagccggtgc
     1021 cgatcaattt cgaaggtgct gcgaccgctg accacccacc gggcttttac ggaccgccgg
     1081 atcaattgct ggccgtcaat gctttgcgcg cgacagagac attgcagacg gctgactatt
     1141 ccggcctcgg cttcgcgcaa gaggctttgc ggcaagcggt accgactgat ctgcggccta
     1201 ttctattggc aatcgcgttc ttgctgttca tcgccgattc gctcgcctcg ctctggctgt
     1261 cgggcggcct gtcgcgccgc atcagtcgcg ccgccgcggc gatcattatt atcggcatgg
     1321 ccgcgagcct cgtgcctgtc gcgcacctta gagcggcgcc gcgtgcggac acgcccttgt
     1381 cgcaaagcga tctgcaatcg gcgctcaata cacggctcgc ttatgtcatt agcggcgatc
     1441 cggatgttga tggcgagagc aaagcgggcc tcaccagcct ctcgcaggtt ctggcgcagc
     1501 gcacgtcgct cattcccggc acgcctgtcg gagtcgatcc ggcgcgcgac gaactggcct
     1561 tttatccgat gctctattgg ccgatcgtcg ccgaccggcc gcaaccgtcc gccgcagcaa
     1621 tcgccaaggt cgccgcctat atgaaacagg gcggcacgat catcttcgat actcgcgatg
     1681 cgttgacggc gcatgccgac ggaccgccga cgccggagac gctgtggctg cgtcaaccgc
     1741 tacagggcgt cgatgtcccg cagctcgaac cgatcccgcc ggatcacgtc gtcaccaaga
     1801 cgttttatct catcgatggt ttcgtgggcc gttacgagac aggacagacc tggatcgaag
     1861 ccttgccgcc gctaaaccct gccgatggcc aacgcccggc gcgcgcgggc gatggcgttt
     1921 cacccatcct catcaccgcg aacgatctcg cggcaggatg ggccgccgac gacaacggcg
     1981 acaaccttta tgcactgatg cccggcggcc cgcgccaaca tgaattggcg ctgcgcggcg
     2041 gtgtcaatct cgtgatgtac acgctgaccg gcaattataa gaccgatcag gtgcatgttc
     2101 gcgacttgct ggaacgactg gcgcattagc cagaaagatc gagtatcaac cgcgatagtc
     2161 gatcgcctca tgtcgtgcat tgcggcgcag tacttgcgga ggttctccga atgcgcgcaa
     2221 gaaggcacgc cgcatatggt cgcggttgct gaatcccgtt tggcgtgcga caatatcgat
     2281 gggatgtcgg ctctcttccg tgaggttccg cgcagcctca acccgcaagt tttcgatcgc
     2341 cttcgccggc gactgacctg tttcggcatg gaacgcccgg ctgaattgac gtgggctaag
     2401 atgagccaca tccgcgagct ccggaacggt cagcggtttg tgaaggttat cccgagcata
     2461 ggtgagcgcc atctgaatac gatccgattt cggtgacagc tccaggagcg tggaaaactg
     2521 cgactggccg ccgacgcggc ggtggtagat gaccagcttg cgggccacgg acttcgcaag
     2581 ctcaggtccg aggtcacttt cgatcagcgc cagaccgagg tcgatgccgg ccgtcatgcc
     2641 ggcggaagtc cagatcggtc cgtcgtttat gaatatccgg tcttcatcca tcttcacttt
     2701 tggaaatcgc ctttgcagtt cccttgcgtg aagccagtgg gtcgtggcgt gccgcccgtc
     2761 cagtagacct gcttccgcga gcacgaacgc gcccgtgcag acggcggcga tgcggcgatg
     2821 ccgtttggga gcgtctttta cgtaggcaat caaacccggt gtcgacgacg cggggatcgt
     2881 cgcgccgcca acgacaagag tatcgaacgc cttttcgccg aaagcctcgc tctcgatcat
     2941 catgccggac gaggttttga cgcggccgcc ggtctcggaa acgaagtgca ggccgtaagg
     3001 cgggtcgtcg gctatcgtgt tggcgacttc aaaagccgtg acgacagcca gcgccatcac
     3061 ggaatagccc ggatatacga cgaacgcgat ttcacgcata aagagtgtct cacatgtcct
     3121 aaatcgacgt ttatatgaca tttgagacgt ggcgatgcaa gcgtatctct tcttcgcaga
     3181 gctcggtccc cgagcgacga cggaaggagt acattcatga gcagtccctc tcccaagggc
     3241 accgccctca tcaccggcgc atcgtcgggc atcggcgccg tctacgccga tcgtctggcc
     3301 aagcgcggct acgatcttat tctcgtggcg cgcaacgaac agcggctgaa cgccgtcgca
     3361 agccggcttc gcggggagac cggacgtcag gttacggtgc tggccgccga cctcaatgac
     3421 aaggcaagcc ttgcgaaagt tgaggcggtc ctgcgagatg atccgagcat cattatgctc
     3481 gtaaacaatg ccggcgtcgg ctccgtcgcg ccgatcctgc aagctgacgt cgacaagatg
     3541 gaggcgatga tcgatctgaa catcacggct cttactcgcc taagctatgc cgcagcgccc
     3601 gcgttcgtgg cgcgcggcgc gggtacgatc attaacatcg cctcggtcgt cggcatcgcc
     3661 ttagaagcct tgaatggcgt ctatagcgct tcaaaatcct acgtcctgag tttcggacat
     3721 tcgttgcaga aagatctggc ggaaaagggc gttcgcgtac aaacggttct tcccggtgca
     3781 acagcaacag agttttggga catcgccggc tatccgccgc agaagcactc cgagatcact
     3841 atgtcggccg aggattgcgt cgatgcggca ttagccggcc tcgacgccgg cgagctggtc
     3901 accatccctg gccttcatga aggcgagctg tggacgcgct gggaggcgga gcgtcggggg
     3961 ctttcgatga aattcggaaa cgctaaacct gctcctcgtt acatggccgg agtggataag
     4021 gtcggcgtcg cgtaattcac ttcgcctgca gagcatcgga caattcacgg aaagcatcgc
     4081 gcgagggcga tgaactcggc gattgcgtca aagcatcgta aagccttggc acgagcgcga
     4141 cgatctgatc gagatcggta tcctgtcccg cctgataccc gcccatcagc cggaggtcgc
     4201 gcgtatcctc atagcgcgcg accatggact tcaatttcag gatcagcgtc tgctgttcct
     4261 tggtccagac gtgttgggcg agtcgcgaga tcgaggagag gatattgacg gccgggaagc
     4321 ggccgagatc ggcgatcgcg cgatcgagca caatatgccc gtcgagcgtg ccgagaatat
     4381 tgtcggcgac ggggtcatta tggtcgtcgc catcgacgag aacggagaaa attccggtga
     4441 tcgaaccgct gccttcaacg cccggcccgg cgcgctcgag cagtttgggc agatcgctga
     4501 agacacttgg cgcgtagccg cgcgcaaccg ccggttcgcc cgctgcgagc gcaacgtcgc
     4561 gggccgcatg tgcgaaacgc gttaccgaat cgaggatcag caggacattc tcgccacggt
     4621 cgcggaaaaa ttcggcgatg gtcatcgcgg tcttcggcgc gagacggcgc atcatggggc
     4681 tttcatcgcc ggtcgagacc acggtcaccg ccagcttgcg attttggccg agggaatcgt
     4741 cgagaaattc gcggacctcg cggccgcgct cgccgacgag cccgacgacg acggcgctga
     4801 agcctttagc accggccaac atgccgagca gagttgattt gccgacgccg gagccggcga
     4861 agatgccgat acgctggccg gcacaaagcg gcatgaagag atcgatggcg cgcacgccgg
     4921 tccgcagcga tgtcttcacg cgcgcccgat tcagggcctg cggcggcggc gattcgattg
     4981 agcgggtatg ttcgccacgc cgcagcgcgc cctggccatc gagcggccgc cccagcgcat
     5041 cgataacgcg gcccttccag ctcacatcgg gcgaaagatg caaaggcccg attcgatagg
     5101 cgctgtcgcc aaggcccgca gcggcgccga catcaaaact tttgaccgtc gcgcccgaaa
     5161 cgtcgacacg cacgacttcg cccaattggg tttcgccgtc gtgctccatg ccgacgcatt
     5221 cgccgagttt gagaaattgc gagaggccag tgatccgata atgcgaaggc gcaacttcgc
     5281 tgacaaagcc gccgatgcga agatcgccga tttcctcgag gccgtaattg atggcctgtt
     5341 cgagccgtgc caaagccgtc atcagagagg ccgtaggcga ggaatcacga tgttccgccg
     5401 aggatcggca gcgcgtcctt caaggtgctt tcgttctttt cagagccgcc cgtgacgctt
     5461 tcgaacgccc gctggatcgt catcatcttg gccatttcca tgatcggatt gacgttcgag
     5521 ccttcgatgt ggccctggac gatgccgttg gtattgaagt cgagaatcgg gcttgcgggc
     5581 ctgtcgggaa tcaccccgga attctcataa cggctgaatc gcgcgttagg atcgatctga
     5641 aagagcccga gcgcgccgac ctgattgata ccctgagtga tcattccatc accgccgatc
     5701 accggcggtc cggcgttggg atcgagaatg agcggcgttc cgcctgcatc gaggatcggg
     5761 tggccggcga gcgattcaag ttcgcccgaa gctttcatcc gcatacgccc gtcgcgggta
     5821 tataccgtgc ccgcgggggt ttggattgcg aggaagccct cgccttgcac cgccacatcg
     5881 agcggattgt ccgtgcgctc cagcggcccc tcggtccggg tcacatagtc cttgcctgcg
     5941 gtggcataat cgacgggatc ggcgccggcc cgcgagacat aggtgtggaa gctgacgccg
     6001 gccgcgcgat agcccacagt gttcatattg gcaacattca acgccaagga ttc
//
LOCUS       DASWOG010000328         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_355392,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000328 DASWOG010000000
VERSION     DASWOG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355392"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGB93_06830"
     CDS             complement(<1..226)
                     /locus_tag="VGB93_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEY0145853.1"
                     /translation="MADAVLTFNAGSSSIKFSLFELSSPDRLSLAFKGEVEGIDSAPH
                     FFVHDATGNVLTERRWTGSQPDFQALLETVI"
     gene            complement(230..1663)
                     /locus_tag="VGB93_06835"
     CDS             complement(230..1663)
                     /locus_tag="VGB93_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEY0145854.1"
                     /translation="MYAGKIASPDSVIENRTFDEIAVGEKASLSRTVTQQDIDLFAVV
                     SGDVNPAHMDPVYAKTDIFHRVIAHGMLGAGLISNVLGTKLPGPGTIYLGQELRFLRP
                     VDIGDTITATLTVTEKHPEKGDLLLDCNCSNQSGVEVISGTAHVRAPRDKVRVSRVEL
                     PEVRVTRHARFNALLAETAGHQPLPTAVAHPCDANALGAAVEAANAGLIAPILVGPRA
                     KILKVAEDAKLDISSFTLVDAPHSNAAAAAAVALVRSGEAALLMKGSLHTDEFLRAVL
                     APGNGLCTARRLSHVYLLDVPGYPRPLLLTDAAVNISPDLEQKRDIIQNAIDLAHVMG
                     IACPKVAILSAVETVSPQLRSTLDAAALCKMADRGQITGGLVDGPLAFDNAVSPVAAA
                     DKGIVSKVAGQADILVVPDLEAGNMLAKQLTFLAGADAAGVVLGARVPIILTSRADAE
                     RTRMASCAVAVLIARSQAAIPQKAIGV"
     gene            complement(1679..3400)
                     /locus_tag="VGB93_06840"
     CDS             complement(1679..3400)
                     /locus_tag="VGB93_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0145855.1"
                     /translation="MSKVAAVAEAPCETEDSLDRLLHAREARFTFSQSLESLTLAFLD
                     WGLHLANAPGRRITLAESASRQWARLLDQDLWAKPASGDHRFDDEGWAHPPFNTFAQA
                     TILAEQWWNEATACLPGVAKSHSAVVSFAARQVLDLYSPSNFPLTNPEVLRATAAESG
                     RNFIRGYRNFSEDAQRVARGLPMDEVKGFAVGEEVAVTPGKVVFRNELIELIQYQPTT
                     ETVRPEPIFIVPAWIMKYYILDLSPHNSFIRYLVSQGFTVFCVSWRNPGPELRNTSFD
                     DYRRLGVMAALDAVTAISGAKKVHACGYCLGGTLLSIAAAAMARDKDDRFATVTLLAA
                     QTDFTEAGELQLFTDEGELALLDDVMWQQGYLDSTQMAGAFQILRSNELIWSRLIKTY
                     LLGGREKPFDLMAWNADATRMPFRMHSEYLRAMFLHNDLAEGRYRVDGRPVAISEIHA
                     PMFAVTTETDHVAPWRSVYKILLLNQGDITLVLTSGGHNAGIVSEPGHPRRHYRLARR
                     PSQAPYMAPDDWKAAAPEYEGSWWPALAEWLGERSGDWVSPPPLADPAKGYPALADAP
                     GTYVRET"
     gene            3781..>4146
                     /locus_tag="VGB93_06845"
     CDS             3781..>4146
                     /locus_tag="VGB93_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145856.1"
                     /translation="MLDKAKSEAGIAEPKEQAQFPVNFVEAQKRTARLFAETSELAAK
                     AAREIIEHQTELLRLETEQIVTAFSPQKIGEDSGATAVARFDQWQEQTDLMIAQARQL
                     NDIVRDYGWQMLKLCASGYH"
BASE COUNT          916 a         1216 c         1185 g          829 t
ORIGIN      
        1 cgatgaccgt ctcaagaagc gcctgaaagt cgggctgtga gcccgtccaa cgccgctcgg
       61 tcagaacgtt accagtggcg tcgtggacaa aaaaatgtgg cgccgagtcg attccttcga
      121 cttcgccttt gaaagcaagg ctcaatcgat ctgggctcga aagttcaaag agcgaaaatt
      181 tgatgctcga tgacccggca ttgaaggtca agacagcatc ggccacggtt caaaccccaa
      241 tcgctttctg cggtatggcc gcttggctcc tggcgatcag caccgcgacg gcgcaggatg
      301 ccatgcgggt gcgctctgca tcggcgcggc tcgtgaggat gatcggcacc cgtgcgccaa
      361 gcaccacgcc ggctgcgtcg gcgccggcga gaaacgtgag ctgttttgcc agcatattgc
      421 ccgcctccag atccggaacg accagaatgt cggcctgccc tgcgaccttg gagacaatcc
      481 ccttgtccgc agcagcgacg ggactgaccg cgttgtcaaa ggcaagcggt ccatcgacca
      541 agccgccggt gatctggccg cgatctgcca tcttgcagag cgcagccgca tcaagcgtcg
      601 agcgaagttg cggacttaca gtctccacag ccgacaggat tgcgaccttc gggcatgcga
      661 tccccatcac atgtgcaaga tcgatagcgt tttgaataat gtcacgcttc tgctcaaggt
      721 ccgggctgat gttcactgca gcgtcggtca gcaagagcgg gcgcggatag cctggcacat
      781 ccagcagata gacgtggctg aggcgccgcg ccgtgcataa accgttgcca ggcgcgagca
      841 ccgcgcgcag aaattcgtca gtatgcagcg atcccttcat caggagcgcc gcctcgcctg
      901 agcgcacaag cgcaacggct gctgcggctg ccgcattgct atgcggcgca tcgacaagcg
      961 tgaaggatga aatgtcgagc ttggcgtctt ccgccacctt gagaattttt gcccgcggtc
     1021 cgacaagaat cggcgcgatg agtccggcgt ttgccgcttc aactgccgcg cctagtgcgt
     1081 ttgcatcaca agggtgagcg acggcggtcg gaaggggctg gtgtcccgct gtctcggcga
     1141 gaagcgcgtt aaatcgggca tgacgtgtca cccgaacttc aggcagttcg acgcgcgaca
     1201 cccgtacttt atccctcggg gcgcgcacat gggccgtacc gctgatgacc tccaccccgc
     1261 tctggttgct gcaattgcag tcgagcagca gatcaccttt ttcgggatgc ttttcggtta
     1321 cggtcaacgt tgcggtaatc gtgtcgccga tgtccaccgg acgcagaaat cgtaattcct
     1381 ggccgagata aattgtgccg gggcctggga gcttcgttcc aagcacgttc gaaatcagac
     1441 cggcgcccaa catcccatgt gcaatgaccc ggtggaaaat gtcggtcttc gcatagaccg
     1501 ggtccatatg cgccggattc acatcgcctg agacgactgc gaagaggtcg atgtcttgct
     1561 gcgtcacagt gcgggagagg gatgcctttt cgccgaccgc gatctcgtcg aaggtccggt
     1621 tttcgatcac cgagtcaggt gaggctatct tgccagcata catgcgccat ccctccgctt
     1681 aggtctcgcg cacgtaggtt cctggcgcgt cggcaagtgc cggatagccc ttcgccggat
     1741 cggcaagagg cggcggcgaa acccaatcgc cagaccgctc gcccagccat tcagccaaag
     1801 ccggccacca ggagccctca tattccggcg ccgcggcctt ccaatcgtct ggcgccatat
     1861 agggggcctg gctcgggcgg cgcgcgaggc gataatgccg gcgcggatga cccggctcgc
     1921 tgacgattcc cgcgttatgg ccaccggacg taagaaccag ggtgatatca ccctggttca
     1981 gcagaaggat tttgtagact gatcgccacg gcgcgacatg atccgtctct gtcgttacgg
     2041 caaacatcgg cgcatggatc tcggagatcg caactggccg gccgtcgaca cgataacgtc
     2101 cctcggccaa atcattatgc aggaacatcg cgcgcagata ttccgagtgc atccgaaatg
     2161 gcatccgcgt cgcatctgcg ttccacgcca tgagatcgaa cggcttttcg cgcccgccga
     2221 gaagataggt cttaatgaga cgtgaccaga tgagttcgtt ggatcgaaga atttggaagg
     2281 cgccggccat ctgggtgcta tccagatagc cttgttgcca catgacatcg tcaagcagcg
     2341 ccagctctcc ctcatcggta aagagttgca gctcgccggc ttcggtgaaa tcggtttgtg
     2401 cggcgagcaa agttacggtt gcaaaacgat catctttgtc gcgagccatc gccgcggccg
     2461 caatcgaaag caatgtgcca ccgaggcaat agccgcacgc atgcaccttc ttggcgccag
     2521 agatagcagt aaccgcatcg agcgcagcca tcaccccaag acgccgataa tcgtcaaacg
     2581 acgtgttacg aagctccgga cctggattgc gccaggaaac gcagaagacc gtaaaaccct
     2641 gagaaacgag atagcggatg aaagaattgt gcggcgatag gtcgagaata taatatttca
     2701 tgatccaggc cgggacgatg aagatcggtt cggggcgaac cgtctcggtc gtcggctgat
     2761 attgaatcag ttctatcagc tcattgcgga acaccacctt gccgggcgtc accgcaactt
     2821 cttctccgac cgcgaagcct ttgacctcgt ccatgggaag gccgcgcgcg acccgctgcg
     2881 cgtcctcgct gaaattccga tagcccctga taaaattccg gccggattcg gcagccgttg
     2941 cccgcaatac ctctggattc gtcaagggaa agttggacgg cgaatagaga tcgagaacct
     3001 gacgcgcggc gaaggatacc acggcgctat gcgacttcgc cacgcccggc aggcaggcgg
     3061 tcgcctcatt ccaccattgc tcagccagga ttgtggcttg cgcgaaagta ttgaatggcg
     3121 gatgcgccca gccttcatca tcaaaccggt gatctccaga tgccggcttc gcccataaat
     3181 cttggtccaa caggcgtgcc cattgacgcg aagcgctttc ggctaaggtg atacgccgcc
     3241 ccggcgcatt ggcgagatgc agaccccaat ccaaaaaagc gagtgtcaac gattctagag
     3301 attgcgagaa agtgaaacgc gcctcacgtg catgcaatag acgatcgagc gaatcttctg
     3361 tttcacacgg cgcttccgcc acggcggcga ccttactcac ctgagttgcg tttttgtttg
     3421 ccacggcaat cgcgctctgg gttttttccg catttgattc cagatttgct tgcggacttt
     3481 tcgttgaggc atcagtcgtg ccgcttttag caaggcctgt tctcaacatg cgtaactcct
     3541 tcaacgtcga tgccccaaag ccatgacaac gagatgaata tctcaaccgg ctttgcaacc
     3601 atgctgcacg gtattcagga atcgacgcag gcgaattgat gccgatcaat caagggcgac
     3661 catctgcgcc aaacggcctc ggtaatatct ccacgacact tgatcgaaat caaatccctc
     3721 tgctcgtgtt cgcctatgtt cgcacgaggt gactaacacc ttataatctt ggaggacgac
     3781 atgctcgata aagcaaaatc cgaagccggg attgcggagc cgaaagaaca agcccaattt
     3841 cccgtcaact ttgtcgaggc tcaaaagaga acggcaagat tattcgctga gacgagcgag
     3901 cttgcggcga aggccgctcg ggagattatc gagcatcaga ccgagttgtt gaggctcgag
     3961 accgaacaga tagtgacggc cttttcacca caaaagatcg gagaagattc gggtgcgacg
     4021 gcggtcgctc gtttcgatca atggcaagag cagaccgacc tcatgattgc ccaagcgcgg
     4081 caactcaatg acatcgttcg cgattacggc tggcagatgc tgaaactatg cgccagcgga
     4141 tatcat
//
LOCUS       DASWOG010000329         6686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_208768,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000329 DASWOG010000000
VERSION     DASWOG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208768"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGB93_06850"
     CDS             complement(<1..334)
                     /locus_tag="VGB93_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY0145857.1"
                     /translation="MYRNLFSVAVLTLVSRATGFIRDVMLAAVLGAGLNADAFVVAFR
                     LPNHFRAIFGEGAFNAAYVPCYSQILAKRGAEAGRHFASQIFTLLLASQIVLLVAALI
                     FMPQFIDLL"
     gene            complement(490..1089)
                     /locus_tag="VGB93_06855"
     CDS             complement(490..1089)
                     /locus_tag="VGB93_06855"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY0145858.1"
                     /translation="MALTLPPLPYAYDALQPYMSRETLEYHHDKHHKAYVDTANNLLK
                     DSPLADKPLAEIVKESHGKNAPLFNNAAQAYNHTHFWHWLKPNGGGPIPGKLEKALAD
                     SFGSVEEAKKQLVQGGVGQFGSGWTWFALKDGKIEVMKTANGENPLIFGAIPILGIDV
                     WEHSYYIDYRNRRLDYLQAVVDHLVNWEYVAERFESGEA"
     gene            1364..2257
                     /locus_tag="VGB93_06860"
     CDS             1364..2257
                     /locus_tag="VGB93_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145859.1"
                     /translation="MKYALFVLGMILIVVGGYFLFSGSNIIEVERGWASVIAGTTALT
                     GGVITLGLAGVVKSLEDLQRLLAAGVERPAAGPLLLETPAPSFTAIETPVAPDFGPVL
                     FDADAPSLFEAEVARDELPEPPAPDVIPSPLPKERPRLTPKFERSEDTAEPSAAIAAL
                     RQRVADDFSLNWPSFNQRPEPTVPTEPAPTAPDPAPVTPPPLHLDDLDIDEPELLPRE
                     PEAETHELEAEPAPTAPEFAIAEADGASAPEAPKPKAIGRYEADGTIYVMFADGSIEA
                     QSVGGTRHFNSMAELKASFHA"
     gene            2531..2944
                     /locus_tag="VGB93_06865"
     CDS             2531..2944
                     /locus_tag="VGB93_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEY0145860.1"
                     /translation="MSDTNSGNYIELAAEIVSAYVSNNSVPASDLPSLIHEVHSALLR
                     VTSGHPIVVAEAPKPAIPTKKSITNEYLICLEDGRKFKSLKRHLRTQYNLSPEEYREK
                     WGLPPDYPMVAPNYAKARSNLAKQMGLGQQRRRKR"
     gene            complement(2954..3385)
                     /locus_tag="VGB93_06870"
     CDS             complement(2954..3385)
                     /locus_tag="VGB93_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY0145861.1"
                     /translation="MSLDEIIENFSYLDDWEDRYRYVIELGRTVEPIDEAARTDANKV
                     LGCASQVWLETHVDRSGQGEPILSFRADSDAHIVRGLIALILALYSGKTSADILATDA
                     EALFDKLGLSAHLTRQRTNGTRSMVERIKRDARRAAEKTVA"
     gene            4224..6035
                     /locus_tag="VGB93_06875"
     CDS             4224..6035
                     /locus_tag="VGB93_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0145862.1"
                     /translation="MSFALNIEKQITSLVHPNVLPHPAERLRHETFILRRLASSIAVI
                     CLAPLFLAIYGAPALWHALVFVWCIVPIGAVLLVSRSGNLLLAQAICVVSFIGAAASI
                     AATGGPWEAALVWLVLAPFEGALSQNSRLVLGAGALSATAALGLILMRFGFSSDHASA
                     LMLPAIAYATFVAYGLVAFQRRRETSVHARSEYYRAVYEMVGDLIVHHASDGTAEFVS
                     PACYELFGVPSADLLGRGLFEHIHVADRPAFLKAISDAATSSRVATATLRLRAPGPHG
                     EAGADADFRWIELRAQHCSSEAEDATARSGHVVSMLHDITQIKQREQDYEAARATAED
                     ANLWKDQFLANVSHELRTPLNAIIGFSEILSDEGLSPQEPGKQREYAAIIHKSGQHLL
                     AVVNTILDMSKIQSGAFEIRAEPFPLPPLIDLCCDMVKLKATEGSVELVRAYPEKIDA
                     IIGDKRAYRQVLLNLLSNAIKFTPPRGRVTIKLRPDGNHIIISVADTGIGIAQNDLAS
                     LGSPFFQAGASYDRPYEGTGLGLSVVRGLVGLHGGTISIESEPDRGTCVSVRLPLDSR
                     RLPASKTGTAKIETLPRRSRGDDQRVFSNEMMVKKIA"
     gene            6028..>6686
                     /locus_tag="VGB93_06880"
     CDS             6028..>6686
                     /locus_tag="VGB93_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY0145863.1"
                     /translation="MREALARSDNDFLVAEPRRRKPAARKSSLSKLIGVARFLGGVAN
                     YPNRVAGALLVAMAVAITVNALELQTTRHPAPLFGHATTAQVEAAPAIAPAPTARVVL
                     PAPESAPMPTPYASAAPVAPIPNADPLGQFIRQDEAPAPAHNKRPSRPAAPHADPISQ
                     ILHEDSRTPDIPPKPSRTVLGVQRALVKLGFVLEPDGIEGDATRKAVTQFDRDHHLPT
                     RA"
BASE COUNT         1386 a         2010 c         1913 g         1377 t
ORIGIN      
        1 ccagaagatc gatgaattgc ggcatgaaaa ttagcgccgc gaccaacagc acgatctgcg
       61 aagccagcag cagcgtgaag atttgactgg cgaaatggcg ccccgcctcg gcgccgcgtt
      121 tcgccagaat ctgcgaatag caaggcacgt aggcggcgtt gaacgcgcct tcgccgaaga
      181 tcgcgcgaaa atggtttggc aggcggaagg ccacgacaaa cgcatcggca ttaaggcccg
      241 cgccaagcac ggcggccagc atgacgtcgc ggataaatcc cgtcgcacgc gaaacgagcg
      301 tcagcactgc gacggagaaa aggtttctat acatccaagc gtccgccccc gcgaatcgcg
      361 gaggaccacg atctttttat ttcagcgcca agtcaaaata cacgccgttg cgaccaaagg
      421 tacacaaaat gaaaggccgg ccaagtgctt ggccgacctt ttatcgccgc aattctggca
      481 aaacctagtt taagcttcgc cggattcgaa gcgctcggca acatattccc aattgacgag
      541 gtgatcgacg acagcttgca gataatcgag acgacgattg cggtaatcga tgtaatagga
      601 atgttcccag acatcgatac caagaattgg tattgcaccg aagatcagcg ggttttcgcc
      661 attggcggtc ttcatgactt cgattttgcc gtccttgagc gcgaaccacg tccagccgga
      721 gccgaattgg ccgacgccgc cctgaacgag ctgcttcttc gcctcttcaa cagagccgaa
      781 tgagtcggcg agcgcttttt caagcttgcc gggaattggg ccaccgccat tcggcttcaa
      841 ccaatgccag aagtgggtgt ggttataagc ttgggcagca ttgttgaaga gcggcgcatt
      901 cttgccgtgt gattccttga cgatctccgc caagggtttg tccgccagcg ggctgtcctt
      961 caggaggttg ttggccgtat cgacataagc cttatgatgc ttatcgtgat gatattcgag
     1021 ggtttcgcgc gacatatagg gttgtagcgc gtcgtaagca taaggcaggg gtggaagcgt
     1081 gagagccatg tctactcctt attggcatgg agacggggaa actcatgaag cggctgcaat
     1141 atcccatggc gaaaggatcg ggccgcgtat gtcaggtagg aaggcgggac ggttcggcaa
     1201 ccttatactg ggcaaaggtg cgaaccggaa gcctggttga gcgacatctg acgtcctcca
     1261 gccaaccctt cccttccgtt agcgatacca caacggatgg caggcaatgt gacaggccaa
     1321 gtcaaatgct cgcgctgact tttaaagcac ggaggcggaa atcatgaaat acgccctttt
     1381 cgtcctcggc atgattctca tcgtggtcgg tggctatttc ctcttcagcg gctcaaatat
     1441 catcgaagtc gagcgtggct gggcaagtgt cattgcgggg acgaccgcat tgacgggcgg
     1501 cgtgattacc ttgggccttg cgggagtcgt caagagcctc gaagatttgc agcggctttt
     1561 ggcagccggc gtcgaacggc ctgcggccgg ccccttgctc ctggaaacac cagcgccgtc
     1621 ttttaccgcg atcgaaacgc ccgtcgcccc cgacttcggc ccggtgctgt ttgacgccga
     1681 cgcgccctct ctctttgaag cagaggtggc gcgggacgaa ctgccggaac cgcccgcgcc
     1741 agatgtgatc ccgagcccgc tcccaaaaga gcggccacga ttgacgccca aattcgagcg
     1801 ttcagaagat acagcagagc catcagccgc gatcgccgca ttgcgtcaac gcgtggcaga
     1861 cgatttttcc ttgaattggc cgagtttcaa tcaacgcccc gagccgacag tgccaaccga
     1921 acctgctccc accgcacctg acccggcgcc ggtgacacca ccgcccctgc atctcgacga
     1981 tctcgacatt gatgagccgg agctattgcc gcgggaaccg gaggccgaaa cgcatgagct
     2041 ggaagccgaa cccgcgccta cggctccaga atttgcgatc gctgaagcag acggagcttc
     2101 tgcgccggaa gcacccaagc cgaaggccat tggccgctat gaggctgacg gcacgatcta
     2161 cgtgatgttc gctgatggat cgatcgaggc acaatccgtg ggagggacgc gccatttcaa
     2221 ttcaatggcc gagcttaaag catctttcca cgcctagact tggccatacc tgcaattgca
     2281 accatctaat ttagattctg acgtaagggc agctaagttt tccagtggag ttttacaatc
     2341 ttagactgtc gggcagttgt gccgcgatag ttttcatttt atcacctcgg tgtgattcct
     2401 gaaaagtcgc gtgacaaatc gcattgcaat ggtttttcaa aagattttac gtggatgcga
     2461 cgcaatatcc gcggtgcgtc cgttactttt tgatcaatgc tgactgaaaa ttaaaagcag
     2521 gggccgagaa atgagcgaca caaattctgg aaactacatt gaactggcgg ccgaaattgt
     2581 ttcagcctat gtcagcaaca actctgttcc tgcgagtgat ctgccaagtc ttattcatga
     2641 agttcattcg gcgctgctga gagtcacctc tggacacccc atcgtcgtgg ccgaggcgcc
     2701 caagcccgcc ataccgacaa aaaagtcgat taccaacgaa tatcttatct gcttggaaga
     2761 cggtcggaaa ttcaagtcgc tcaaacggca cttacggact cagtataatt tgtcgcccga
     2821 agagtatcga gagaaatggg gcttaccccc ggattatcct atggtcgcgc cgaactacgc
     2881 taaggcccgt tccaaccttg ccaagcagat gggcctcggc cagcagcgcc ggcgcaagcg
     2941 gtaatccctc cagctaagcc acagtctttt ctgccgcacg ccgtgcatcg cgcttgatgc
     3001 gttcgaccat cgagcgcgtg ccgtttgtac gttggcgcgt caaatgcgcg gacagaccga
     3061 gcttatcgaa aagcgcttcc gcgtcggtgg cgagaatgtc cgccgaggtc ttgcccgaat
     3121 aaagcgccaa aatcagcgcg atgaggccgc gcacgatatg cgcgtcactg tcggcgcgga
     3181 agctgaggat cggctccccc tgcccgctgc gatcgacatg agtttcaagc caaacctggc
     3241 tagcgcagcc gagcaccttg ttggcatcgg ttcgcgcagc ttcgtcgatc ggctcgaccg
     3301 tacgcccaag ctcaattacg tagcgataac gatcttccca atcgtccaga tagctgaagt
     3361 tttcgatgat ttcgtcgagg ctcatgatcc aatccggcgc gcctgcgccg ggtcagaact
     3421 cggcgcgtcc ctgatccata agatattcat gccgcgctgt cttctccagc gtcggcggag
     3481 ccgttctaac gaagttctgg ggccgccgcg gcggcaaagg cacgtctgca aagtgctgga
     3541 cgtttgccgc gatctgcgtg acctcggcgc tccgccatgc ggatgccggg acgctgctgc
     3601 cagccaattt agagagctgt gcggcgacag cgaggcaagt cgccggcgca ctcccacagc
     3661 ggtcagcaac tctggtggcg gccctctcaa gccaagcttg cgcgctcgag gctatcggct
     3721 gcgtcgctgc agctggcgcc gcggacggcg acgcatacgg agcagcgtgc tcgcccgaag
     3781 gctgcgcaaa gatggtgctg aacaccaccc aaagccagaa aacgcagcgg atcaaaaaga
     3841 acatattcgt ctcgtctcaa gcagccgcat gacgaccgat gcgacaccct agctgcggcg
     3901 cataaaaatc gtgcgattat cggcgcgaca attcgagccg cgagatttac cttttgatga
     3961 ccatttgccg agcgcgcgga aagctatccg gcaagacctt ctaaaccata gcgggagaat
     4021 tcgcgtctcc aggcgccgcg cttcctctcg ggcgcttgat cactgccagc gttttgacgg
     4081 tgcggcttag cgttcactta agacgcgcct gcgaacttgt tttcgtcgaa aggcggagat
     4141 tcgcagacga gttgcgatcc ccttcgcttc gcggccgcgc ccgcagcttg ctgtgccgtc
     4201 gcgcggaagc cggagttcag cggttgagtt ttgcgttaaa tatcgagaag cagatcacct
     4261 ccctggtgca tccaaacgtt ctgccgcatc cggccgagcg cctgcgccat gagaccttca
     4321 ttcttcgccg tttggcgagt tcgatagcgg tcatatgcct cgcaccacta tttctcgcga
     4381 tctatggcgc gccagcgctg tggcacgccc ttgtcttcgt ctggtgtatc gtcccgatcg
     4441 gcgctgttct tctggtatcg cgcagcggca atctgttgtt ggcgcaagcg atttgcgtcg
     4501 tcagtttcat cggcgctgcg gcatcgattg cggcaaccgg cggtccttgg gaagccgcac
     4561 tcgtctggct cgttctggcg ccatttgaag gtgcactttc acaaaattct cggcttgtac
     4621 tcggcgccgg agcgctgtcc gcaaccgcgg cgcttggtct aatccttatg cgcttcggct
     4681 tttcaagcga ccacgcgtct gctctgatgc tgccggcaat cgcctatgcg acgttcgtgg
     4741 cttacggcct tgtcgctttt cagcgccggc gtgagacgtc ggttcacgca cgttcggaat
     4801 attatcgtgc cgtctatgag atggtcggcg acttgatcgt gcatcacgcc agcgacggca
     4861 ccgcggaatt tgtcagtccg gcttgctatg aacttttcgg cgtgcccagc gccgatctct
     4921 tgggccgcgg cctctttgaa catatccacg tcgccgaccg gccggcgttt cttaaagcga
     4981 tttccgatgc cgcgacatca tcgcgcgttg cgacggcgac cctgcggttg cgagcgccgg
     5041 gtccgcacgg cgaggcgggc gccgacgctg actttcgctg gattgagttg cgcgcgcagc
     5101 attgctcatc cgaggccgag gatgcaacgg cgcgctcggg gcacgtcgtg tcgatgttgc
     5161 acgacatcac gcagattaag cagcgcgagc aggactatga ggctgcacgc gccaccgccg
     5221 aagatgcaaa tctttggaaa gatcaattcc ttgccaatgt cagccatgag ttgcgcacgc
     5281 cgctcaacgc gatcatcggc ttttcagaga ttctgtccga cgagggattg tccccacagg
     5341 agcccggcaa acagcgcgaa tatgctgcga tcatccacaa atccggccag catctgctcg
     5401 cggtcgtaaa taccatcctc gatatgtcga agattcagtc tggcgcgttc gagatcaggg
     5461 cggagccttt cccgttgccg ccattgatcg atctgtgctg cgatatggtg aaactcaaag
     5521 cgaccgaagg tagcgtcgag cttgtacgcg cctatcccga gaagatcgac gcgatcatcg
     5581 gcgacaaacg ggcgtatcgg caggttcttc ttaatctgct ttccaacgcg atcaagttca
     5641 cgccgccgcg cggccgtgtg acgatcaagc tgcggccgga tggcaatcac atcatcatct
     5701 cggtcgccga taccggcatc ggcatcgcgc agaacgatct cgccagtctt ggcagtccat
     5761 tctttcaagc tggcgcatcg tatgatcggc cttacgaagg cacggggctc gggctttctg
     5821 tcgtgcgcgg cctcgtcggc ctccacggcg ggacgatctc gattgagagc gaacctgatc
     5881 gtggaacctg cgtcagcgtc cgcctgccgc tcgacagtcg ccgcctgccg gctagcaaga
     5941 ccggcacagc caaaatcgaa accttgccgc gccgctcgcg cggtgacgat caacgtgttt
     6001 tcagcaacga gatgatggtg aagaaaattg cgtgaagctc ttgcccgcag cgacaatgat
     6061 ttcctcgtcg ccgagccgcg ccgcagaaaa cccgccgcgc gaaaaagttc tctgagcaag
     6121 ctgatcggcg tcgcccggtt tctgggcggc gtcgcgaatt atccaaatcg cgttgccggt
     6181 gccttgctcg tcgcgatggc agttgccatt acggtcaatg cactcgaatt gcagacgaca
     6241 cgccaccctg caccattgtt cggccatgcg acgacggccc aggtcgaagc tgcgccggcg
     6301 atagcgccag ccccgaccgc gcgcgttgtg cttccggctc cagagtcggc gcccatgcca
     6361 acgccttacg ctagcgctgc gccggtcgcg cccatcccca atgcagaccc tctcggtcaa
     6421 ttcatccgcc aggacgaggc gcccgcaccg gcccataaca aacggccgtc acggccagcc
     6481 gcgccgcacg ccgacccgat ctcccagatc ctgcatgagg acagtcgaac cccggatatt
     6541 ccgccaaagc cgagccggac ggtcttgggg gtccagcggg ctctcgtgaa actgggcttt
     6601 gtattagagc ccgacgggat tgaaggcgac gccacacgca aggccgtcac gcaattcgac
     6661 cgcgatcatc acctgccgac gcgagc
//
LOCUS       DASWOG010000330         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_153873,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000330 DASWOG010000000
VERSION     DASWOG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153873"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..390)
                     /locus_tag="VGB93_06885"
     CDS             complement(136..390)
                     /locus_tag="VGB93_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEY0145864.1"
                     /translation="MTADIAETGVAGEELKQFVERIERLEEEKKALSDDIREVYGELK
                     GRGFDSKAVRQIVKIRKQDHAERKEMEAILDLYMQALNMA"
     gene            complement(524..1330)
                     /locus_tag="VGB93_06890"
     CDS             complement(524..1330)
                     /locus_tag="VGB93_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0145865.1"
                     /translation="MAKELSGDGFEPVERIEGRADAGVLLLCDHASNALPQSYGTLGM
                     APADLAAHIGYDIGAADLTRRLAAQLGAPALLTRFSRLLIDPNRGAQDPTLVMRLSDR
                     RLIPGNARIDAVEIGRRRALYWQPYRVAIDDMIAELSAQGPIPAVISMHTFTPVWHGT
                     ARPWQIGILWDNDPRLPRPLMAALEARGVVVGDNEPYDGALPGDTLYEHVTARGLAGL
                     LIEVRQDLVATPETAHGWADLLADVLPPILKRPEMHEIALGSSRAACGRR"
BASE COUNT          274 a          505 c          482 g          314 t
ORIGIN      
        1 aagggcaatc cggccgagac ggcggatcgc ccatgaatgc cgcgcctcag ctgcggcagg
       61 ctcgacatag gaacgtcttc aggaatttgt aagcgggcac gcgccccggg ccggcttcac
      121 acgatctcgt gaagctcacg ccatattgag cgcctgcata tagagatcga gaattgcctc
      181 catctccttg cgttcggcat ggtcctgctt acggattttg acgatctggc ggaccgcctt
      241 gctgtcgaag ccgcgccctt tcagctctcc gtaaacttcc cggatgtcgt cgctcaatgc
      301 ctttttttct tcttccagcc gttcgatccg ctcgacgaat tgcttcaatt cttcgcccgc
      361 gacgccggtc tcggcgatat ctgccgtcat gtcagactta tcctgatgtc tttgtttgtt
      421 agctgtgcgg gccatagcag cctccacgat ttttcaacgt cccgcttgcg gccccagcgc
      481 cccaaaatca agcttgcggc ggcgaaatcc ccgctattct ggtctatcgg cggccgcagg
      541 cggcgcggct tgagccaagg gcgatctcgt gcatctccgg ccgtttcagg atcggcggca
      601 gcacgtcggc cagaagatcg gcccagccat gcgccgtctc aggggtcgcg acgagatcct
      661 gccggacttc gatgagaagg cccgcgaggc cgcgggcggt gacatgctcg tacagcgtat
      721 cgcccggcag tgcgccgtcg taaggctcgt tgtcgccaac gacaacgcca cgcgcctcga
      781 gcgccgccat caacggccgc ggcaaacgcg gatcgttgtc ccagagaatg ccgatctgcc
      841 agggccgcgc cgtgccgtgc cagacggggg tgaacgtgtg catcgaaatc accgccggga
      901 tcgggccctg cgccgagagc tcggcaatca tgtcgtcgat ggcgacgcgg tagggttgcc
      961 aataaagcgc ccggcggcgg ccgatctcga cggcatcgat gcgggcattg ccgggaatca
     1021 gccggcggtc cgaaagccgc atgaccagcg tcgggtcctg tgcgccccga ttggggtcga
     1081 tgagcaggcg cgaaaagcgc gtcagcaggg cgggcgcgcc gagttgcgcc gcgagccttc
     1141 gggtgagatc ggcagcgccg atgtcgtagc caatatgtgc cgcaaggtcg gccggagcca
     1201 tgccaagcgt gccatagctt tgcggcagtg cgttgctcgc atgatcgcag agcagcaaga
     1261 cgcccgcatc cgcgcgtcct tcaatccgtt cgaccggctc gaaaccgtcg ccgctcaatt
     1321 ctttggccat gggctatttt caaagggcga atcgcgtgga gtcaatctcc acacaccgcc
     1381 ggatcgagct ttggatgacg cggcgccgca tccagatttc gcttgcctgc cgggcgcgga
     1441 ggcccgctat tctggctcaa ttggcgcgcg tcccgggatg gccattgttg cgtcaaagtt
     1501 ttcttcttgg tctagaaccc tccacatgag tccatcacgc aaaacattca ggcgcggctt
     1561 gacgcttcta atcct
//
LOCUS       DASWOG010000331         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_301182,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000331 DASWOG010000000
VERSION     DASWOG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301182"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VGB93_06895"
     CDS             complement(<1..791)
                     /locus_tag="VGB93_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY0145866.1"
                     /translation="MKLPRELGPIHFVGIGGIGMSGIAEVLLNLGYKVQGSDASENAN
                     VLRLREKGATIYIGHNAANLGEAAVVVVSTAIKRDNPELVAAREKRLPVVRRAEMLAE
                     LMRLKQCVAIAGTHGKTTTTSLVATLLDAGKFDPTVINGGIINAYGTNARLGAGDWMV
                     VEADESDGTFLKLPADIAIITNIDPEHLDHFKTFDAIKAAFFSFIENLPFYGFAVMCL
                     DHPTVQELVGQIEDRRVITYGENPQADVRLVDVDLTGGKSRFNVI"
     gene            complement(788..1894)
                     /gene="murG"
                     /locus_tag="VGB93_06900"
     CDS             complement(788..1894)
                     /gene="murG"
                     /locus_tag="VGB93_06900"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0145867.1"
                     /translation="MSSGPVFVAAGGTGGHLFPAEALARELTARGVAVELLTDSRGKK
                     YGGDFPAQAAHEIPSATPSGGGIFARAKAILTLLFGTLWALWLIGRHRPSAVVGFGGY
                     PSVPPVFAATLLRVPTILHEQNAVMGRANRFLAARVDAVAHGFPTLNGLGPGIKAPTY
                     HTGNPVRPAVLAAATVPYRVPDDGTFDLLVTGGSQGARVMADIVPPAIALLSPDERKG
                     LKIVQQARGEDQARVAQAYEACAVAAEVLPFFADLPARVAAASLVIARAGASTVSELA
                     VIGRPSILVPFPHALDQDQAANAAELATTGATQVIREADFTPQWLADTLRAARQNPEG
                     LASRAAAAQKAGISDAAKRLADLVINIALGNKKA"
     gene            complement(1905..3044)
                     /locus_tag="VGB93_06905"
     CDS             complement(1905..3044)
                     /locus_tag="VGB93_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEY0145868.1"
                     /translation="MVSRVERSTLANWWWTIDRWLLAALGTLVIFGLVLTMAASPPVA
                     ERLGLPTFHFVNKQVMFLIPSLAVFFGASLLSPRHVRRAALIVFLVSMALIFAALLFG
                     EEVKGARRWIFGIQPSEFMKPGFVVVAAWAFSEGGRDRLPGNLIALLLLPATIIPLIL
                     QPDFGQTMLVSLVWVGLFFVAGLRWIWVLGVGGIGMGGVLLAYKFVPHVRARILKFID
                     PGATGGVADTFQVDTALQSFFAGGWTGKGPGEGTIKRILPDAHTDFIFAVTAEEFGII
                     ACLVIVAVFMFIVVRGLTVASRNEDQFCRLAGSGLVMLFGIQSAINMAVNLHLMPAKG
                     MTLPFISYGGSSLISLSLGMGFLIAVIRKRPRSEVIFRKIGAEAA"
     gene            complement(3056..4450)
                     /gene="murD"
                     /locus_tag="VGB93_06910"
     CDS             complement(3056..4450)
                     /gene="murD"
                     /locus_tag="VGB93_06910"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0145869.1"
                     /translation="MTPITSFVGKRVAVFGLGASGLISAQALVAGGADVVVWDDHRPG
                     REKAAVAGLKLEDFVTADMTGIESLVLAPGVPLTHPYPHWIVVKAKAAGVEIIGDIEL
                     FVRERAHIAPKSPLVAITGTNGKSTTTALIAHLYQTFGYEVEVGGNIGVPVLALNPPS
                     DKRVHVIECSSFQIDLAPSLNPSFGVLLNITVDHLDRHGTMEHYAEIKERLVAKADVA
                     IVGVDDDICAGIAQKLRAQGRTIVPISIHHPVIPDGIVLEGTRLVRKQGGVASSIADL
                     DSIPSLRGTHNGQNAAAAVAALGPHGFELELVQKGLRSFPGLAHRMEEIGTRGKVLYI
                     NDSKATNADAAEKALLSLDDIYWILGGRAKEGGIGPLQPLFHKVAKAYLIGEAAEPFA
                     KVIGEAIPYEFCGTIDRAVESATRDAEAASTPAPIVLLSPACASFDQFPNFEKRGDKF
                     REVVLAKLAEPPKV"
     gene            complement(4450..5451)
                     /gene="mraY"
                     /locus_tag="VGB93_06915"
                     /pseudo
     CDS             complement(4450..5451)
                     /gene="mraY"
                     /locus_tag="VGB93_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174752.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
BASE COUNT         1183 a         1676 c         1687 g          905 t
ORIGIN      
        1 atgatgacat tgaagcgcga cttgccaccc gtcagatcga catcgacgag acgtacgtcg
       61 gcctgaggat tttcgccata ggtaatgacg cgccggtcct caatctggcc gacaagctcc
      121 tgcaccgtcg gatgatcgag acacatcacg gcgaaaccgt agaacggcaa attctcgatg
      181 aaggagaaaa atgcagcctt gatcgcatcg aacgtcttga aatgatcgag atgctcggga
      241 tcgatattgg tgatgatcgc aatatcggcg ggcagtttca ggaacgtgcc gtcgctttcg
      301 tcggcttcga caaccatcca atcgccggcg ccgagacgcg cgttagtgcc ataggcattg
      361 atgatgccgc cattgatcac ggtcggatcg aatttgcccg catcgagcag cgtggcgaca
      421 agtgaggtcg tcgttgtctt gccatgcgtg cccgcaatcg cgacgcattg tttgagccgc
      481 atgagctcgg ccagcatttc ggcgcgacgg acgacaggta gacgtttttc acgcgcggcg
      541 acaagttcgg gattgtcgcg cttgatcgcg gtcgagacga caaccactgc ggcctcacca
      601 agattcgcgg cattgtggcc aatataaatt gtggcgccct tctcgcgcag gcgcagaaca
      661 ttagcgtttt cgctcgcgtc ggaaccttga actttatagc cgagattgag caacacttcg
      721 gcgatgccag acatgccgat gccgccgatg ccgacgaaat gaattggtcc cagttcgcgc
      781 ggcagcttca tgcttttttg tttccgagag cgatattgat cacgagatcg gcgagcctct
      841 tggcggcgtc gctgattccg gctttctggg ctgccgcggc gcgcgacgca agcccttcgg
      901 gattctggcg cgctgcgcgc agcgtatcgg cgagccattg cggcgtgaaa tccgcctcgc
      961 ggataacttg cgtggcgcct gttgtcgcca gctcggccgc attcgccgcc tgatcctgat
     1021 cgagcgcatg cggaaacggg acgaggatcg aaggccgccc gatcaccgcc agttccgaga
     1081 cggtcgacgc gccggcccgc gcaatcacca gcgaagccgc cgcaacgcgc gccgggagat
     1141 cggcaaaaaa cggcagaact tccgccgcga ccgcgcacgc ctcataggct tgtgcaacgc
     1201 gggcctgatc ctcgccgcgc gcttgctgca cgatcttcag gcccttcctc tcatcgggcg
     1261 acaaaagcgc gatggccggc ggtacgatat cggccatcac gcgtgcgcct tgcgagccac
     1321 ccgtgacgag cagatcgaac gtgccatcgt ccgggacacg atatggcact gtggccgccg
     1381 cgagcaccgc agggcgcacg ggattaccgg tatgataggt cggcgccttg atgcccggcc
     1441 caaggccatt gagcgtcggg aagccatgtg ccaccgcatc gacgcgtgcg gcaaggaagc
     1501 gattggcgcg gcccatcaca gcgttctgct catgcaggat cgtcggcacg cgcagcagcg
     1561 tcgcggcaaa gaccggtggc acgctcggat aaccgccgaa gccgacgacc gctgacgggc
     1621 gatgacggcc gatgagccaa agcgcccaca atgtgccgaa gagcagcgtc aggatcgctt
     1681 tggcgcgggc gaaaatgccg ccgccgctcg gcgtcgccga gggaatctca tgcgccgcct
     1741 gggccggaaa atcgccgccg tactttttgc cgcgcgaatc cgtaagaagt tcgacggcga
     1801 cgccccgcgc cgtcaattcc cgcgccagcg cctcggcggg gaagagatgg ccgccagtgc
     1861 cgcccgccgc gacgaagacc gggccggaac tcacgattcg atcctcacgc ggcttcggct
     1921 ccgatcttgc ggaagatgac ttccgaacgt ggccgtttgc ggatcacggc gatcaggaag
     1981 cccatgccga gcgagagcga gatcagcgac gagccgccgt aggagataaa cggcagggtc
     2041 atgcccttgg ccggcatcag atgcagattg accgccatat tgatcgcgct ctgaatgccg
     2101 aacagcatca caaggccgga accggcaagg cggcagaatt gatcctcatt gcgcgacgcg
     2161 accgtaaggc cgcgcacgac aatgaacata aagacggcga cgatcacgag gcaggcgatg
     2221 atgccgaatt cctctgccgt cacggcgaag atgaagtccg tgtgtgcatc gggaagaatg
     2281 cgcttgatcg taccttcgcc cggccccttg ccggtccagc cgccggcgaa gaagctttgc
     2341 aaggcagtat cgacctgaaa cgtgtcggcg acgccgcccg tcgcccccgg atcgatgaat
     2401 ttcagaatgc gggcacgcac atgcggcacg aatttatagg caagcaaaac accgcccatg
     2461 ccgatgccgc cgacgcccag aacccagatc cagcgcagac cagcgacgaa gaaaagcccg
     2521 acccagacga gcgagacaag catcgtctgg ccgaagtcag gctgcaggat gagcggaatg
     2581 atcgtcgccg gcagaagcag caatgcaatc agattgccgg gcagacgatc gcggccgccc
     2641 tcggaaaacg cccaggccgc cacgacgaca aagcccggct tcatgaattc cgatggctgg
     2701 atgccaaaaa tccagcgccg agcgcccttc acctcctcgc cgaacagaag cgcggcgaag
     2761 atcaaggcca tcgacaccag aaacacaatg agcgcggcgc ggcgcacgtg gcgcggcgac
     2821 agcagcgatg cgccgaagaa gaccgccagc gaggggatga ggaacatcac ctgcttgttg
     2881 acgaaatgga acgtcggcag gcccagccgt tcggcaacgg gagggctcgc tgccatcgtc
     2941 aggacgaggc cgaagatgac cagcgtaccg agtgcggcga gcagccagcg gtcgatcgtc
     3001 caccaccaat ttgccagggt cgagcgttcg acgcgcgaga ccatatctac ccccttcaga
     3061 ccttgggcgg ctccgcaagc ttcgccagaa cgacctcccg aaacttgtcg ccgcgtttct
     3121 cgaaattcgg gaactgatcg aacgaggcgc aggcgggcga cagaagcacg atgggcgccg
     3181 gtgtcgaggc cgcctcggca tcgcgcgtcg cgctttcgac cgcgcgatcg atcgtgccgc
     3241 aaaattcata aggaattgct tcgccgataa cttttgcgaa aggctccgcc gcctcgccga
     3301 tgagataggc cttggcgacc ttgtgaaata acggttgaag cgggccaatt ccgccctcct
     3361 tggcgcgacc gccgagtatc caatagatat cgtcgaggct cagcagcgcc ttttccgccg
     3421 cgtcggcgtt ggtggccttc gaatcattga tgtagaggac cttgccgcgg gtgccgatct
     3481 cctccatccg atgcgcaagg ccggggaagc tccgcaaacc cttctggaca agctcaagct
     3541 cgaaaccgtg cgggccaagc gccgctacag ccgccgccgc gttctggccg ttgtgagtgc
     3601 cgcgcaatga aggaatgctg tcaagatcgg cgatggacga cgccacgccg ccctgcttgc
     3661 gcacaagccg cgtgccttcg aggacaatgc cgtcgggaat gaccggatga tgaatcgaga
     3721 tgggcacgat tgtccggccc tgcgcgcgca gcttttgcgc aatgcccgcg caaatgtcat
     3781 cgtcgacgcc gacgatggcg acatccgcct tggcgacgag ccgttccttg atctctgcgt
     3841 aatgttccat cgtcccatgc cggtcgagat gatcgaccgt gatattcaag agcacgccga
     3901 acgacggatt gagcgacggc gcaagatcga tctgaaatga cgagcactcg atcacatgga
     3961 cgcgtttgtc tgatggcgga ttaagcgcga gcactggaac gccgatgttg ccgccgactt
     4021 cgacctcata accgaaggtc tggtagagat gcgcgataag cgccgtcgtc gtcgatttgc
     4081 cattcgtgcc ggtaatggca acgagcggcg atttcggcgc gatatgggcg cgctcgcgta
     4141 caaaaagttc gatatcgccg atgatctcga cacccgccgc cttcgccttg acgacgatcc
     4201 aatgcggata gggatgggtc agcggcacac ccggcgcaag cacgagggac tcgatgcctg
     4261 tcatatccgc cgtgacaaag tcttcgagct tgagaccggc gacggcggcc ttttcgcggc
     4321 cgggacggtg atcgtcccaa acgacgacat ctgcgccgcc agcgacgagc gcctgggcgc
     4381 taatgaggcc ggaggcaccg aggccgaaga cggcgacgcg cttgccgacg aaggacgtga
     4441 tgggtgtcat tagcgcaact tcaatgtcga aaggccgacg agggcgagga caaaggcgat
     4501 gatccagaac cgcaccacga cctgcggctc ggaccagccg agctgctcga aatgatgatg
     4561 gatcggcgcc attttgaaca cgcgcttgcc ggtcaattta aacgatatga cttggacgat
     4621 aaccgacaaa gcctcgacca caaaaagacc accgacaatc gcgagtacga tctcatgttt
     4681 gaccgcgacg gccaccgtgc cgagcagacc gccaagggcc agcgagccgg tgtcgcccat
     4741 gaaaatttgc gccggcgggg cgttgaacca cagaaagccg aggccggcac caatgagcgc
     4801 gccgcagacg acggcaagct cgccggtgcc cggcacataa ttgatgcgca gatattccga
     4861 atagatcgcg ttgccgacga cataggcgat gatgccgaag gcggccgccg cgatcatcac
     4921 cgggacaatg gcgagaccgt cgagaccgtc ggtgagattc accgcattgc cggcgccgac
     4981 gataatgagc ggtgcgaaaa ttagaaagac atagccgagg tcgatcacgt agcccttgat
     5041 cgccggcagc gcgagttcag tcgtatgcgc ggcacccact ttcatcatcg cgaaacaggc
     5101 gacaatcgca atgccggctt ccagcgccaa gcgcagcctg ccggaaaacc ccttgtgcga
     5161 ctgcttggtg accttgaggt aatcgtcata gaagccgatg aggccgaagc ctacggtaac
     5221 caacaggacg atccagacgt agcggctggc gagattcgcc cagatgagcg tcgagacgat
     5281 gagacctgaa aggatcatca ggccgcccat cgtcggcgtg ccctttttgg tgagcagatg
     5341 cgactgcggc ccgtcttcgc ggatcggctg acctttgcct gcttcagccg caaggccgca
     5401 atggtgcttg gcccgaagaa gaagacgaag aaaagcgccg tggccgtcgc g
//
LOCUS       DASWOG010000332         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_135978,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000332 DASWOG010000000
VERSION     DASWOG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135978"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VGB93_06920"
     CDS             complement(<1..1492)
                     /locus_tag="VGB93_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0145870.1"
                     /translation="MSRPALAASPTTAFRPVDLGPLDVAIELGRDGDFYARSPFALRN
                     YEAKATLWLDRWAALRPDHIFLGERRHGAWETITYAETRRKAHALAQALLGRGLSAER
                     PLAILSGNSIAHALLSLAALYAGIPHAPLSPAYSLASQDFARLRNIVDLLTPGLVFAE
                     DGALYAQALASLPPEMPRLVCTSPAPDADIFDTLLTTKETSALDRANMTVGPETIAKF
                     MFTSGSTAAPKAVVTTHRMLCANQAMIAHVLRFLDSGPPVLVDWLPWSHSFGGNQNFN
                     LVLAHGGTLYIDGGRPTAEGIATTLANLRDVSPTLHFNVPAGLEALLPHLAHDRTLAA
                     NFFRHLRLTFFAGAPLARATYGAWNDMALAACGEKIVTMSGYGATESGPATTFWTPFI
                     RAEGGVGLPLPGCDLKFVPHDEKLEVRVKGPNVTPGYWRDVDATSQAFDDEGFFRLGD
                     ALRFGEPQRPKLGFVFDGRINEDFKLATGIWVSTGPMREAFLAAFAP"
     gene            1610..1684
                     /locus_tag="VGB93_06925"
     tRNA            1610..1684
                     /locus_tag="VGB93_06925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Gln,seq:ttg)
BASE COUNT          349 a          525 c          565 g          303 t
ORIGIN      
        1 aaggggcgaa agcggcaaga aaggcctctc gcataggccc cgtgctgacc cagatacccg
       61 tcgcgagttt gaagtcctca ttgatccgtc catcgaaaac aaagcccaat ttgggtctct
      121 gcggttcgcc gaagcgtagc gcgtcgccga ggcgaaagaa gccttcgtcg tcgaaggctt
      181 gcgatgtcgc gtccacgtcg cgccaataac ccggcgtcac attcggtccc ttgacccgga
      241 cttccagctt ttcgtcatgc ggcacgaact tcaggtcgca gccgggcagc ggcaggccga
      301 cgccgccttc cgcgcgaatg aaaggcgtcc agaacgttgt cgccggtccc gattcggtcg
      361 cgccatagcc gctcatcgtt acgatctttt cgccgcaggc cgccaacgcc atgtcgttcc
      421 acgcgccata agttgcgcgc gcgagcggcg cgccagcaaa gaacgtcagc cgcaagtgac
      481 gaaagaaatt tgctgcaagc gttcggtcgt gggcgagatg cggcagaagc gcttcaagac
      541 cggcgggaac gttgaaatgc aatgtcggcg agacatcgcg cagattggcg agcgtcgttg
      601 cgatgccttc cgccgtcggc cgcccgccgt cgatataaag cgtgccgccg tgcgcgagca
      661 cgagattgaa attctggttg ccgccgaaac tatggctcca tggcaaccaa tcgacgagca
      721 ccggcggccc gctgtcaaga aagcgcagca catgtgcaat catcgcctga ttggcgcaaa
      781 gcattctatg cgtcgtgacg acggccttcg gcgcggcggt cgaccccgac gtgaacatga
      841 attttgcgat cgtctcaggg ccgaccgtca tattggcacg atcgagcgcc gacgtttctt
      901 tggttgtgag gagagtgtcg aaaatgtccg cgtcgggcgc aggcgatgtg caaacgagac
      961 gcggcatctc cggcggtagc gacgcaagtg cttgcgcgta aagcgcgcca tcctcggcga
     1021 agacaaggcc gggcgtcagc aggtcgacaa tattccgcag ccgcgcaaaa tcctgcgagg
     1081 cgagggaata ggccggcgac agcggcgcgt ggggaatgcc cgcataaagc gccgcgaggc
     1141 tcagaagcgc atgcgcgatg ctgttgccgg acaggattgc aagcggccgc tcggcgctta
     1201 ggccgcgccc caacaaggcc tgcgcaagcg catgagcttt gcggcgcgtc tctgcgtaag
     1261 tgatcgtttc ccaggcgccg tggcgccgtt cgccaaggaa tatatgatcc ggccggagcg
     1321 ccgcccagcg atcgagccag agggtcgcct tggcctcata atttcggaga gcaaagggcg
     1381 agcgcgcata aaagtcgcca tcgcgtccca gctcaatcgc gacatcgagc ggaccgagat
     1441 cgacagggcg gaaagcggtg gtcggcgacg cggcaagagc tgggcgggac atgcgcgcta
     1501 tttaacagtt cgcgcggcag gaggaagagg ttgcgagggg cttgggctgg ctctatggtc
     1561 gcgccgccgc cgggccctgc ggcgcttcta ggggccgatt tccacgtttt ggggcgtcgc
     1621 caagtggtaa ggcagcggat tttgattccg ccatccggag gttcgaatcc tcccgcccca
     1681 gccagctcac gacctctcca tggaattcat tgaagaaacg gcgtttcggc tgaatggccc
     1741 ta
//
LOCUS       DASWOG010000333         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_62415,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000333 DASWOG010000000
VERSION     DASWOG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62415"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..97)
                     /locus_tag="VGB93_06930"
                     /pseudo
     CDS             complement(1..97)
                     /locus_tag="VGB93_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            complement(114..410)
                     /locus_tag="VGB93_06935"
                     /pseudo
     CDS             complement(114..410)
                     /locus_tag="VGB93_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007795458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(432..1178)
                     /locus_tag="VGB93_06940"
     CDS             complement(432..1178)
                     /locus_tag="VGB93_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145871.1"
                     /translation="MSNLSGKTALVTGASRGIGRASALALARQGAQVLVHYGRAADEA
                     DAVVAEIRKAGGRAEALSADLAKPDAPHKLAKDVRAIVGERLDILVANAGISKAVKIE
                     DTTIEDFDNLFAVNVRAPFFLVQQLLPILGQGGSVIFVSSVVAHAVVGQLYAYAATKG
                     AVDTLVKYFAASLGERGIRVNAVAPGVVDTDMSSFTKTEAGRNTAMSLQALKRIAGPD
                     DIADVIAFLASDEARWITGDIVRVDGGSKL"
     gene            complement(1202..1594)
                     /locus_tag="VGB93_06945"
     CDS             complement(1202..1594)
                     /locus_tag="VGB93_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY0145872.1"
                     /translation="MSSAAAKSIKIPGPDHPITTAHNPNRVIVTFAGRVIAETQDALT
                     LKEANYPPVIYVPRKDANMALLKPTEHTTYCPYKGDGSYFTISAGGREAVNAVWSYEH
                     PHPAMAAIADYLAFYPDRVDAIEERLGA"
     gene            complement(1642..>2024)
                     /locus_tag="VGB93_06950"
     CDS             complement(1642..>2024)
                     /locus_tag="VGB93_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0145873.1"
                     /translation="ELRAMLPKGSGVVVNVSSAYGHEGAAGASVYVASKHAVEGLTKS
                     AALETATTGVRVVAVAPGPITTGMLERFTGDANGMAAIAASVPMKRVGLVENIADAIV
                     FISSPKAAYITGTVLSVDGGMAAA"
BASE COUNT          372 a          615 c          627 g          410 t
ORIGIN      
        1 cggaatattc cacggtgaag accgtatcct ccggcagttc gacgaattgc cctagaaagg
       61 cgaaattggc gctgcctttc gggatcaaca gtggcttctt cgaacttctt tgggatcgcg
      121 gtgccggcgt tgttcacgaa cacgtcgagc ttaccaaaag cggcgaccgt cttctcgacc
      181 gccgcgttga ccgcatgcgg gtcggtggca tctgcctgaa cggcgatcgc cttgccaccg
      241 ctgcgctcga tatccgcaac caccgccgcc gcggcgtcgg cgcctttcgt ataggtgatc
      301 gccaccttgg caccatccgc ggccagacgt ttggcgattg cagcgccaat gccgcgcgag
      361 ctaccggtga cgaatgcaac tttaccttca agtttcttgg acatcatcat tctccgtttg
      421 agattcattg ctcacagctt cgagccgcca tcgaccctga caatatcgcc ggtaatccag
      481 cgcgcctcat ccgaggcaag gaaagcgatg acgtctgcga tatcgtccgg gcccgcaatg
      541 cgctttagcg cttgcaggct cattgcggta ttgcggccgg cctccgtctt ggtgaagctc
      601 gacatatccg tatcgacgac gccgggcgcg acagcattga cgcgaatgcc gcgctccccg
      661 agcgacgcgg cgaaatattt gacgagcgta tcaaccgcgc ctttggtcgc cgcataagcg
      721 taaagctgac cgacaacggc atgcgccacg acggacgaga caaagatcac gctgccgccc
      781 tggccgagga tcggcagcaa ttgctgcacg aggaagaagg gcgcacggac attgacggcg
      841 aagagattgt caaaatcctc gatcgttgtg tcttcgattt tcacggcttt ggaaatgccg
      901 gcattggcga cgagaatgtc gagccgttcg ccgacgatgg cgcgcacatc cttggcgagc
      961 ttgtgcggcg cgtcgggttt ggcgagatcg gcggaaagtg cttcggcgcg gccaccggcc
     1021 ttgcggattt ccgccacgac cgcgtcggct tcatccgccg cgcggccata atgtacgaga
     1081 acctgcgcgc cttgcctggc gagcgccagg gcactggcgc ggccgatccc gcgcgaggcg
     1141 cccgtaacga gcgcggtctt cccggacaaa ttgctcatgg tctgatgtcc tttccgcgtg
     1201 gttaggcgcc gaggcgttct tcgattgcgt cgacgcgatc gggatagaag gcaaggtagt
     1261 ccgcgatggc cgccatcgcc ggatgcggat gctcgtagct ccagacggcg ttgacggcct
     1321 cgcgcccacc ggcggaaatg gtgaaatagc tgccgtcgcc cttgtaaggg caataggtcg
     1381 tgtgctcggt cggcttcagc agcgccatgt tcgcgtcctt gcgcgggaca taaatcaccg
     1441 gcgggtagtt cgcctctttc agagtgagag catcctgcgt ctccgcgatg acacggccag
     1501 cgaaggtcac gatcacccgg ttcgggttat gcgcggtcgt gattggatgg tcgggaccag
     1561 ggattttgat cgatttggct gctgcgctgc tcatgtcatc acctctgacg tttgagttta
     1621 agtgtgcgta tggcgttcga cctatgcggc cgccataccg ccatcgactg atagaacggt
     1681 gccagtgata taggcggcct tgggcgagga gatgaatacg atcgcatcgg caatattctc
     1741 gaccaggcca acgcgtttca tgggcacgct cgccgcgatg gctgccatgc cattggcatc
     1801 gcccgtgaag cgttccagca tgccggtcgt gattgggccg ggcgccacag cgacgacacg
     1861 gacacccgtt gtagccgtct caagtgcggc agacttggtc agcccttcga cggcatgctt
     1921 gctcgccaca tagacggagg cgccggctgc gccctcgtgc ccgtaggccg aggagacatt
     1981 gacgacgaca ccgctgccct tgggcagcat ggcgcgcaat tcat
//
LOCUS       DASWOG010000334         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_117647,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000334 DASWOG010000000
VERSION     DASWOG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117647"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..569)
                     /locus_tag="VGB93_06955"
     CDS             complement(108..569)
                     /locus_tag="VGB93_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145874.1"
                     /translation="MSGSRTLSFRVVRRGALALTGSLLASLTIIPANAQYGPPPWAYP
                     YYHDYHGGYPSPPGPPGDEGPPGPDYPDGGYEAPRSFTVADVRRRVTRLGLHLVAKPR
                     RKDNIFLAEAEDANGVAHRMVFDADSGHLIENTKLPPRKDTAHKAAPEAGQ"
     gene            928..>1905
                     /locus_tag="VGB93_06960"
     CDS             928..>1905
                     /locus_tag="VGB93_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEY0145875.1"
                     /translation="MTAVEKHQRIGSELGRQTAGKAAFSTGAGLDPGLPPAQGLYDPR
                     QEHDACGVGFIANMHNQKSHDIIGKGLEILLNLDHRGAVGADPKAGDGCGMLVQMPHR
                     FFAAEADKLGFRLPALGEYAAGLIFLPRDPEIRSKIESLIASVVAAEGQVLLAWRDVP
                     RDSSGLGESIKPTEPVIRQIFIERGLDAGRPGADYDEFERRLFILRKVISNSVRDLND
                     PRTKGFYIVSLSSRTIVYKGLLLATQLGEYFKDLQDPLFESALALVHQRFSTNTFPTW
                     QLAHPYRMVAHNGEINTVRGNNNWMAAREASVSSPLFGDDIKKLWPISYD"
BASE COUNT          362 a          550 c          592 g          401 t
ORIGIN      
        1 aggcagtcga gaccaattcg tcgccgaaag ccgcgtaatc tcgcgtacac tgtcccagcg
       61 gatcggggag ggcgccgaag cggcgccctc ttcacgtcaa cgccctttta ttgaccggct
      121 tccggcgcgg ctttgtgcgc cgtgtctttg cggggtggca gtttggtgtt ttcgatcaga
      181 tggccgctat ccgcgtcaaa caccatccga tgggcaacgc catttgcgtc ctccgcttct
      241 gcaaggaaaa tattgtcctt gcggcgcggt ttcgcgacga gatgaagacc aagcctcgtt
      301 acgcggcggc gcacgtctgc aaccgtgaaa ctccgcggtg cctcatagcc gccgtccggg
      361 tagtcagggc ccggcggccc ttcatcgccc ggaggacctg gcggtgaggg atagccgcca
      421 tgatagtcat ggtaataagg gtaggcccac ggcggcggcc catattgtgc gtttgctgga
      481 ataattgtga gcgaggccag aagactgccg gtaagcgcaa gggcgcctct gcgcacgaca
      541 cggaagctta gcgtacgaga gccagacatg tgtcctccat ccggtcggcg tctatcataa
      601 ccttcacaag aattccggcg aaagtcggca tggattacgg cggcgcggcg atccgcggcg
      661 cttgggtgaa gaaaggcaaa gagatgcgcc gagcgcggtt gattttaccg cggtgacatt
      721 gcggcgcgat attttgccct cgggaccaaa gtgtgcttgt gttttcattc taaatttgaa
      781 aaagtccgcc gcggatagga cagccttgtt gaccttttaa gatgctggca cgtttccagc
      841 attttctagt cgcagagttg gtcgcgacgg cgggcattct cagacaatgg gcgagttcga
      901 cccggctttg agttttgggg acggagcatg acggctgtgg aaaagcatca gcggattggg
      961 tcggaactcg ggcgtcagac ggccggcaaa gccgcttttt cgaccggtgc cggtctcgat
     1021 cccggcctgc cgccggcgca aggtctttac gacccacgcc aggagcatga tgcgtgcggt
     1081 gtcggcttta tcgccaatat gcacaatcag aagtcgcacg acatcatcgg caagggcttg
     1141 gaaatcctcc tcaacctcga tcaccggggt gctgtggggg ccgacccaaa ggctggcgac
     1201 ggttgcggca tgctggtgca gatgccgcat cggttctttg cggcggaagc tgacaaactc
     1261 ggcttccggc ttccggcgct cggcgaatac gccgccggtc tcatcttcct gccgcgcgat
     1321 cccgaaatcc gcagcaagat cgaatcgctg attgcatcgg tcgttgccgc cgaggggcag
     1381 gttttgctcg cgtggcgcga cgtgccacgc gattcgtctg gacttggcga gagcatcaag
     1441 ccgaccgaac ccgtcatccg gcagattttc attgagcgcg gcctcgacgc agggcggccg
     1501 ggtgccgact acgatgagtt cgagcggcgt ctgttcatcc tgcgcaaagt gatctcgaac
     1561 agcgtgcgcg atctcaatga tccgcgcacc aagggattct acatcgtctc cctgtcgtcg
     1621 cgcacgatcg tttacaaagg tctgctgctc gcgacgcagc tcggtgaata tttcaaggac
     1681 ctgcaggatc cattgttcga atcggcgctg gcgcttgtgc atcagcgttt ctcgaccaac
     1741 acgttcccga cttggcagct cgcgcatcct taccggatgg tcgcgcataa tggtgagatc
     1801 aacaccgtgc gcggcaataa caattggatg gcggcgcgcg aagcgtcggt cagctcgccg
     1861 ctgttcggcg acgacatcaa gaagctttgg ccgatttcct acgac
//
LOCUS       DASWOG010000335         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_99577,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000335 DASWOG010000000
VERSION     DASWOG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99577"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGB93_06965"
     CDS             <1..267
                     /locus_tag="VGB93_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145876.1"
                     /translation="GGGGFHGGGFHGGGFHGGGWGHGGGWHGGYGYGRGYGYGRGWGW
                     GGAGLGLGLAAGALAAPYYYGGGYGYPCGYYRCGGYYGYPYYPY"
     gene            609..797
                     /locus_tag="VGB93_06970"
     CDS             609..797
                     /locus_tag="VGB93_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145877.1"
                     /translation="MRADLGYHSLPVEKSAYVPLAQGPAVALTRNSDDECVSATKVVT
                     VAQGPYGGLYVPRGMTCE"
     gene            852..1874
                     /locus_tag="VGB93_06975"
     CDS             852..1874
                     /locus_tag="VGB93_06975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0145878.1"
                     /translation="MQTADLLKLEPAGLYCAAGDFFIDPLRPVPRALITHAHADHARP
                     GHARVLATAETLAIMAIRYGDNFAGATQAASYGEVLNLGGVDVSFHPAGHVLGSAQIL
                     LARGGARTVVSGDYKRERDPTCESFVPLACDTFISEATFGLPVFRHAQTHVEIDKLLA
                     SQKIFPERTHLVGAYALGKAQRVIALLREAGYDPPVYIHGALEKITAYYQAVGIDLGD
                     VRKAAGAGALPGEIVLCPPSAVNDLWSRKFAEPLTAFASGWMRIRAHARQRGIELPLV
                     ISDHADWDGLCTTISETGSRTLLVTHGEEDALVHCFRQRGLQAEPLHILAYGDEDADN
                     AAETEA"
     gene            1871..>2224
                     /locus_tag="VGB93_06980"
     CDS             1871..>2224
                     /locus_tag="VGB93_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0145879.1"
                     /translation="MNRFAALLDRLGYEPSRLGKLRLMTDYFRQAGDPDRGFALAALT
                     GALNFRHAKPALIRTLIYERVDPVLFDLSYDFVGDLSETVALLWPTDAGLTDNISPSL
                     TQVIETLNTASKLDLP"
BASE COUNT          373 a          696 c          676 g          479 t
ORIGIN      
        1 ggcggcggcg gcttccatgg cggtgggttc cacggtggcg gcttccacgg cggaggctgg
       61 ggtcacggcg gtggctggca cggcggttac ggttatgggc gcggttacgg ttacgggcgc
      121 ggttggggtt ggggcggagc cgggctcggt cttggtctcg cggctggcgc ccttgcggcc
      181 ccgtactatt acggcggtgg ctatggttat ccctgcggct attatcgctg cggcggctac
      241 tacgggtatc cttactaccc ctattaagct ttcctgagag atctggaccg tttcaacggt
      301 tgacgatatg cgcggcaagc catcccggtg gcttgccgtt ttatttggcc ccgtgaaggc
      361 ccggcttacc cctttgcact tgtcacctgc gggcgaggca accccgcggc cccatgtgcg
      421 ttagcgcacc gacccccgga ccccccgccc tattgtcggg gtgcggcgtt agaggttgag
      481 tggaggggca ggcaatgtcg tcccggcaaa tcctcgatgg tagtgtaatt cgttctctgg
      541 cggtgggcat gggtttgtca tgcttggctg cagcattagt tttcggttca ccaattttcg
      601 gttcgccaat gcgagcggat ctcgggtatc acagccttcc ggttgagaag agcgcctatg
      661 tacctttagc tcagggtccg gctgtggcgc tgactcgcaa cagcgacgac gaatgcgtca
      721 gcgcgaccaa agtcgtcacc gtggcgcaag gcccctatgg cgggctttat gttccgcgcg
      781 gaatgacttg cgaataagtt aaaagattgc gccagctttc gcggcgggaa ctgcgtgctt
      841 atctttaagg tatgcaaacc gccgatcttc tgaagttgga acccgccgga ctttattgcg
      901 cggcgggcga tttttttatc gatccgctgc gcccggtgcc gcgcgcactc atcacccatg
      961 ctcatgccga ccacgcgcgt cccggtcacg cgcgagtgct ggctaccgct gagacgcttg
     1021 cgatcatggc gatccgctat ggcgacaatt tcgccggcgc gacgcaagcc gcgagctatg
     1081 gcgaggtgct caatctcggc ggcgttgacg tgagttttca tcctgccggc catgtgctgg
     1141 gctccgcgca aattctactc gcacgtggcg gcgcccggac cgtcgtctcg ggtgattata
     1201 aacgcgagcg cgatccgact tgtgaatctt tcgttccgct cgcctgtgac acgtttatca
     1261 gcgaagcgac attcggcctc ccggtcttcc ggcacgcgca gacccatgtc gagatcgaca
     1321 aactgctcgc ctcgcaaaag atttttccgg aacgcacgca tcttgtcggc gcttacgcgc
     1381 tcggcaaggc acaacgcgtg atcgcactct tgcgtgaggc cggctacgac cccccagtct
     1441 atatccacgg cgcgttggaa aagatcaccg cttattatca agccgtcggc atcgacctcg
     1501 gcgatgtgcg caaggcggcc ggtgccggcg cattgcccgg cgagatcgtt ctgtgcccgc
     1561 caagtgccgt caacgatctc tggagccgga aattcgccga acccctgacc gccttcgcct
     1621 ccggctggat gcgcatccgc gctcacgcac gccagcgcgg catcgaattg ccgctcgtca
     1681 tttccgatca cgccgattgg gatggcctct gcacaaccat cagcgaaacg ggcagccgga
     1741 cgctcctcgt cacacatggc gaggaagacg cgctggtgca ttgcttccgt caacggggtc
     1801 tacaggccga accgttgcat attctggcct atggcgatga agacgccgac aatgcggccg
     1861 agaccgaggc gtgaaccgct tcgccgccct cctcgaccgt ctcggctacg agccatcgcg
     1921 cctcggcaaa ttgcgcctga tgacggatta tttccggcaa gctggcgatc ccgaccgcgg
     1981 ctttgcgctc gcggcgctga ccggcgcctt gaattttcgc cacgccaaac cggcgctgat
     2041 ccggacgctc atctatgagc gggtcgatcc cgtgcttttc gatctctcct acgatttcgt
     2101 cggcgatctg tcggaaactg tcgcgctgct ttggcccaca gatgctggac taacggacaa
     2161 catctctccc agcctgacgc aggtcataga aacgctcaac acagcaagca agctcgattt
     2221 gccg
//
LOCUS       DASWOG010000336         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319626,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000336 DASWOG010000000
VERSION     DASWOG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319626"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1422
                     /locus_tag="VGB93_06985"
     CDS             106..1422
                     /locus_tag="VGB93_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145880.1"
                     /translation="MPKYSWMALVGVSLLSGCGSMTGPPSITASRTFYNEVIHDTSSE
                     QLLLNIVRAKNYETPNFIDVSEADATVSFTSGVNGGLSGIGAVPGIRSTSAGTIAGQV
                     GGVTGNFNYADSALVRYAPVQGYPLIQQVSSPIAPVSIVHMFNSDFELADVLEFSVDR
                     LAPGYTDNYVAQDRMVMLDQFGAIVLAAGAEETSAGPGRHGESASARPSRESAEDSST
                     QNLNVILHPGSLHPSGFIAGRDCAFAEQSPDAAVTKLWSGLRQLYRGSAPNMITLRGA
                     ATSDFFFTTRSAIGALKEGEVDTIHFATPEEAAEIRALNDAAPCRINANLFYFNPPAD
                     KRTPDEVNVMWSSYLADALVPRVDHDIRARTIWRDTHRAFIIVEQGPGVPQNAYVAVN
                     KRGIWYWIDNDDLVSKANFGLLNEIVTIQAVPETRQPLTPSISVGR"
     gene            complement(1435..2601)
                     /locus_tag="VGB93_06990"
     CDS             complement(1435..2601)
                     /locus_tag="VGB93_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145881.1"
                     /translation="MMTAQRAHLFKTSSFIVVCAFALGGTAFVAPTPARAQFGFPFFH
                     LCCSYHHRSHAHHERHHHTDSDDNDDSASSPSSDDSTRELAKLAPPSAHEQMALLKNV
                     NASDSLGAVGSSDDQDASNGAASNDADRDYTTMVDQLMQTLKQRIGESDHQGDVTETA
                     VMDALTEAILQAKLARFETFIGENWSAERLRGMIIKRVSDEIGGLFEGNNRGAVSMAD
                     LDMLIKKAAREVYSRLFETSELLASNRSATLFVQRLYQARGDLVKGDVREDTEELLLK
                     ASSDGVSHFHDKFARADHPYTLIYRAQRIIFDCLSDNVESITASGAGMASTAEIEKRL
                     LGADQNQCQKWVSNQMFGPDGGLKQQEPLPLRVIWTSAGPKDDPSMYGHASDQS"
     gene            complement(2683..>3580)
                     /locus_tag="VGB93_06995"
     CDS             complement(2683..>3580)
                     /locus_tag="VGB93_06995"
                     /inference="COORDINATES: protein motif:HMM:NF022473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2610 domain-containing protein"
                     /protein_id="HEY0145882.1"
                     /translation="DADDAKTVLSDNSLALLWFGRASQSNNPAAQYNLAYMMERGDGL
                     PIPAPEIAARYYRLAAHGGYEDAEIDLAERLRSGRILTNPENGANEAVDLLERALSQG
                     STRAAAYLAEIYRNGEFGYEKNPLKAMQYAYKAIDLSVQADPATEDGDPFFEIDAGIL
                     LAEMAVTGQANDINDKPLLNDDEVARLQKYYGTVNPDVRKVEVRKLEVPLGCAGGYTR
                     PGDIWVWDWGRKEAPTEPQFRSIERQTSCYDNETLRRTLAASFLLARKTKVPFADLIY
                     QQIKEARESVDNGSVSRRRHRY"
BASE COUNT          718 a         1079 c         1011 g          772 t
ORIGIN      
        1 tgcaaaatta caacatccag tcacaatggt ttccgcgcca ttgactcaaa ttcgcgctgc
       61 cgatcatgtg aatggctccg gttgcggtgt gtagtggggg gtgccatgcc gaagtacagc
      121 tggatggcgc tcgtcggcgt aagcctttta tctggctgtg gctcgatgac ggggccgcca
      181 tcgattaccg cgagtcgaac gttttataac gaagtcattc acgatacgtc gtccgagcaa
      241 ttgctgctca atatcgtccg cgccaagaac tacgagacgc cgaatttcat cgacgtttca
      301 gaggccgacg cgacagtctc gtttacgtct ggtgtcaacg gcggcctttc aggtatcggc
      361 gcagtgccgg ggatacgctc caccagcgct ggaacgattg ccggccaggt gggcggggtg
      421 accggcaatt tcaactacgc cgattccgcg cttgtccggt acgcacccgt ccagggctat
      481 ccgctcatcc agcaagtgtc gtccccgatc gcaccggttt cgatcgtgca tatgttcaat
      541 tccgatttcg aactcgccga cgtgctcgaa ttctcagtcg accggctcgc ccccggttat
      601 accgacaatt acgtcgcaca ggatcggatg gttatgcttg accaattcgg tgcgatcgtt
      661 ctcgccgccg gcgccgagga gaccagcgcg ggacccggcc gccacggtga aagtgcgtcg
      721 gcaagaccgt cccgtgaatc cgccgaagac agctccacgc agaacctcaa cgtgatcctc
      781 catccgggaa gtttgcatcc gagcggcttc atcgcggggc gcgactgtgc cttcgcggag
      841 cagtcacccg atgcggccgt caccaaactc tggagcgggc tgcgccagct ttatcgcggt
      901 tccgccccga atatgatcac gctgcgtggc gcggcaacat ccgatttctt cttcactacg
      961 cgttcggcca tcggcgcttt gaaggaagga gaagtcgata cgattcattt cgcgacgcca
     1021 gaggaagcgg ccgagattcg ggcgcttaat gatgccgcgc cctgccgtat caacgccaac
     1081 ctcttctatt tcaacccgcc cgccgacaag cgcacgccgg atgaagtcaa cgtcatgtgg
     1141 agcagctatc tcgcggatgc gcttgtcccg agggtcgatc atgacatccg tgcccgcacg
     1201 atctggcgcg acacgcatcg cgcattcatc atcgtcgagc aaggtccggg cgtcccacag
     1261 aatgcctatg tcgccgtcaa caagcgaggc atctggtatt ggatcgacaa cgacgatctc
     1321 gtgtcgaagg cgaatttcgg cctgctaaac gagatcgtga cgatccaggc cgtacccgag
     1381 acgcgtcagc cgctgacccc atcgatctcc gtcggtcgct aaaactggcc ggcgttagct
     1441 ctggtcggac gcgtgtccat acatgcttgg atcgtccttc gggcctgccg aagtccagat
     1501 gacgcgcagc ggcagaggtt cctgctgctt caggccacca tccggcccaa acatctgatt
     1561 ggagacccat ttctggcact ggttttggtc ggcgccgagg aggcgttttt cgatctctgc
     1621 cgtcgaggcc ataccggcgc ccgatgcggt gatcgattcc acattatctg acaggcaatc
     1681 gaagattatc cgctgcgccc ggtagatgag cgtataggga tgatcggcgc gggcgaattt
     1741 atcatgaaag tgcgaaacgc catccgacga agccttcaac agcaattcct cggtgtcttc
     1801 gcggacgtcg cccttcacca gatcgccgcg cgcctgatag agccgctgca cgaacaaggt
     1861 tgcactgcgg ttcgacgcca acaattcgga tgtctcgaac aagcgggagt aaacctcgcg
     1921 ggcggctttc ttgatcagca tatcgaggtc ggccattgag accgcgccgc gattattgcc
     1981 ttcgaacaga ccgccgatct cgtcgctgac gcgcttgatg atcatgccgc gcaaacgctc
     2041 ggccgaccaa ttctcgccga tgaaggtctc gaaccgcgcc agcttggctt gtaggatggc
     2101 ttccgtcagc gcatccatca ccgccgtttc ggtcacgtcg ccctgatggt cagactcgcc
     2161 gatccgctgc ttcagggtct gcatgagctg atcgaccatc gtcgtgtagt cgcgatcggc
     2221 gtcattgctc gctgcgccgt tcgacgcatc ctgatcgtcc gacgaaccaa ccgcgccaag
     2281 cgaatcgctc gcgttgacgt tcttcagcag cgccatctgc tcgtgagccg acggcggcgc
     2341 gagtttggcg agttcgcggg tcgaatcatc ggacgatggc gacgacgcgg aatcatcatt
     2401 gtcgtcgcta tccgtgtggt ggtggcgctc atgatgcgcg tgcgaacgat ggtgatagct
     2461 gcaacaaaga tggaaaaagg gaaacccgaa ttgggcgcgt gccggggtgg gtgcaacaaa
     2521 cgctgtccca cccaacgcaa aggcgcaaac gacaataaag ctcgatgttt taaacagatg
     2581 tgctcgctgg gcagtcatca tgctctccca accgctaagt atcatttcaa tttaaattat
     2641 accggacggc catcgacttc aactatattt ggacccgacg gattaatatc ggtggcggcg
     2701 gcgtgagacg gagccgttgt ccacgctctc ccgcgcctcc ttgatttgtt gataaatcag
     2761 gtcagcgaag ggaaccttgg ttttgcgcgc gagcagaaag cttgcggcca gagtgcgccg
     2821 cagcgtttcg ttgtcgtagc aactggtctg acgctcaata ctgcgaaatt gcggttccgt
     2881 cggcgcttcc ttgcgacccc aatcccaaac ccaaatatcg cctggccgcg tataaccgcc
     2941 ggcacagcca agcggaacct ccagcttacg gacttcgact ttgcgcacgt cgggattgac
     3001 cgtgccataa tatttctgca gtcgcgcaac ctcgtcgtcg ttcaacaacg gcttgtcatt
     3061 gatatcgttg gcttggccag ttaccgccat ttccgcgagc aagatgcccg catcgatttc
     3121 gaagaacgga tcaccgtctt ccgttgccgg gtccgcctgg actgacaggt cgattgcttt
     3181 atacgcgtat tgcatcgcct tgagcggatt cttctcatag ccgaattcgc cgtttcgata
     3241 aatttccgca agataagccg ctgcgcgcgt cgagccctgg ctcagtgccc gctccagaag
     3301 atcgacggct tcgttggcgc cattttcggg attggtgaga atacgtcccg agcggagtct
     3361 ttcggccaga tcgatttccg catcttcgta gccgccatgc gccgcaagcc ggtaataacg
     3421 cgcggcgatt tcaggcgccg gaatcggcag gccgtcgcca cgctccatca tgtaggcaag
     3481 attatattgt gccgccggat tattcgactg tgaggcccgg ccaaaccaga gaagcgcaag
     3541 cgaattgtcg gacagaactg ttttcgcatc atccgcatcg
//
LOCUS       DASWOG010000337         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_136203,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000337 DASWOG010000000
VERSION     DASWOG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136203"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VGB93_07000"
     CDS             <1..650
                     /locus_tag="VGB93_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145883.1"
                     /translation="GRVCYAGTDARLLSPWQLAARRVVMAQATHLAFPFSVWEVAALG
                     IDRLGRKFPRSRRQDLIIAALEKAEVGHLADRIYQTLSGGEQQRVQFARALTQLWAGA
                     ATNGTPVLFLDEPTAHLDMSHQWMLMDEVRNLVANGVSAFVTVHDLNLAAAYADRIIV
                     MSHGRLIAQGTPNAVLSNELVVKVFGIDSKIGEAAPEGSTFILPHGHAQKKRRPS"
     gene            complement(663..2060)
                     /gene="der"
                     /locus_tag="VGB93_07005"
     CDS             complement(663..2060)
                     /gene="der"
                     /locus_tag="VGB93_07005"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0145884.1"
                     /translation="MFKIAIVGRPNVGKSTLFNRLVGRKLALVDDLPGVTRDRREGVA
                     HLGDLTFTIIDTAGLETGAPATLAGRMRAQTAAAIADADAVLFMMDARAGIVPDDREF
                     AALLRRADKPLILVANKAEGRAGGAGAQEAYDLGFGDPIALSAEHGEGLSDLYDALRE
                     VLPEDAEPSEEELDAAAKEPLGDEEDGSELDPTKPLRVAIIGRPNAGKSTLLNRILGE
                     DRLLTGPEAGITRDAIGVDLVWRDRKLKMFDTAGLRKRARVDEKLEKLANADGLRAAK
                     FAEVVVLLIDATAPFEKQDLTLADLIAREGRALVIGINKWDLVQDRSARLKELREEAG
                     RLLPQIKGMPVVPVSGATGEGIDGLMNAIFAVHETWNIRIATGRLNRWLAAALAANPP
                     PAVSGRRIKIRYMTQLRARPPYFVIFGNQLDHLPESYKRFLTNGIRETFNLKGVPIRL
                     SVRTGENPYDKKKHS"
     gene            complement(2063..2722)
                     /locus_tag="VGB93_07010"
     CDS             complement(2063..2722)
                     /locus_tag="VGB93_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0145885.1"
                     /translation="MADFFREVDEDVRRDQLLDIWKRYQNWIIGAAVLVVLGTGGWRL
                     FDYQRLKSAEAAGGRYAAAEQLLSAGKNSQAAAAFAAVAADAPAGYATLTQLARADAL
                     AAQDPKVGAAAYDEVAANGTVDPAYQEVARLRGAYLRVDSDDPKAFEQRYAAYAGPDS
                     PYRNAYRELLALADTRLGDFASAAHWFELAAADPSSPPALRARADAFLTIVQAGPVPT
                     K"
     gene            complement(2794..3276)
                     /locus_tag="VGB93_07015"
     CDS             complement(2794..3276)
                     /locus_tag="VGB93_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEY0145886.1"
                     /translation="MALIFDGRYTARLERPLVLFLIGMRFNKLILVNKWLPVFLAMPR
                     MLKELGQHPEAGLLSQRTFLSGRVILVQQYWESFEKLVAYAHATDALHFPAWVAFNRE
                     VGKTGTVGIWHETYAAEPGKYETVYANMPRFGLASAAQHVPAVGGLSAAKDRMADLSP
                     "
     gene            3358..4164
                     /locus_tag="VGB93_07020"
     CDS             3358..4164
                     /locus_tag="VGB93_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0145887.1"
                     /translation="MPRKPRIFIGVGGWTYEPWRGSFYPEGLPQKRELEYMSRALTSI
                     EINGTYYGSQKPESFIKWREETPEDFVFSVKGPRFATNRRVLAESGESIERFFASGVL
                     ELKDKLGPINWQFMATKKYDAEDFAKFLVSLPKEIGGQTIRHAIEVRHESFRTPEFVA
                     LAREHGVAVVIAGDSEFPQIADVTADFVYAWIMGTKEKPKIGYPAKTLDQWAERAEIW
                     AEGGAPKDLETITPKAAKTPRDVFLYVISGYKERNPRAAMALIERAGKRI"
     gene            4215..5273
                     /gene="ada"
                     /locus_tag="VGB93_07025"
     CDS             4215..5273
                     /gene="ada"
                     /locus_tag="VGB93_07025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY0145888.1"
                     /translation="MQTTDNTLVQDEQRWAAVSARDHDFDGDFVFAVKTTGIYCRPSC
                     PARHAQRRNVNFFPTCEAAEREGFRACLRCRPQEAPNQRQREAIAAACRALETDEPPT
                     LEVLAARAGLSAFHFHRVFKATTGVTPKAYAKAHRAAKVRQELSEATSVTEAIYAAGY
                     NTSSLFYENADTMLGMAPNKFRQQGSDAKIVYGMGKCSLGTILVARSDKGICALMLGD
                     DAAELESEIKRRFAKAKSIKGDDAFSALLTNVIALADAPEKKLDLPLDIRGTLFQQKV
                     WAALRAIPAGETASYTEIARRIGSPTATRAVAQACAANKIAIAIPCHRVVRSDGALSG
                     YRWGPARKRALLDKERKK"
     gene            complement(5277..6665)
                     /locus_tag="VGB93_07030"
     CDS             complement(5277..6665)
                     /locus_tag="VGB93_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145889.1"
                     /translation="MASFDGLPAPQRYWSAATIWLALALAALDGSIANVALPSIARDL
                     SAPAAESVWVVNAYQLTIVVSLLPLATLGQMLGYRNIFQVGLAFFTAASLGCSFAHSL
                     PELAMARAIQGLGAACIMSVNGALVRYTYPLRLLGRGVGYNALVISAAAALGPTIASA
                     ILAVASWNWLFAVNVPIGILAFLIGRKSLPESPRSGSLDILGAILNVLMFGLGFIGIN
                     AWTQGASDRFGIICVVAATIAAVALVLRSRSETSPIIPLDLLKNREFSLSITTSVASF
                     SAQMLAFVSLPFYFEGILHRDQVATGLLMTPWPVAVGLSAPFAGRLADKFPSAILSGV
                     GLTVLAIGLASLALLSPQASALQIIWRMSLCGLGFGFFQAPNNRTLLSSAPMDRSGAA
                     GGMLATARLTGQTIGATIAALCLRATIQSSAAGLTIAAVLAALAALASLSRVFGREGL
                     RPARPGVMHADE"
BASE COUNT         1451 a         2079 c         2041 g         1197 t
ORIGIN      
        1 ctggacgcgt gtgctacgca ggtacggacg cgcggcttct gtcaccatgg caacttgcgg
       61 cgcgccgcgt cgtgatggca caagcaacgc acttggcctt tccattttcg gtctgggagg
      121 tcgccgcgct tggtatcgat agactaggcc gcaagtttcc ccgcagccga cggcaagatt
      181 tgattatcgc ggcactcgaa aaggctgaag tcggccatct cgcggacaga atctatcaaa
      241 cactctcggg cggcgagcag cagcgcgtgc aatttgcgcg ggcgctcacg caattatggg
      301 cgggagccgc aacaaacggt acgcctgttt tgtttctgga tgagcccacc gcgcatctcg
      361 atatgagcca tcaatggatg ctgatggatg aggtccgcaa ccttgttgca aacggcgtct
      421 ccgccttcgt gacggttcac gatctcaatc tggcggcggc ctatgccgat cgtatcatcg
      481 ttatgagcca cggccgtctc atcgcgcagg ggacgcctaa cgcggtgctg agcaacgagc
      541 ttgtcgtgaa ggtcttcggt atcgacagca aaatcgggga agccgcgcct gagggcagca
      601 catttattct gccgcacgga catgcgcaaa agaaaaggcg gccatcatga caaagagcgc
      661 ggttagctgt gtttcttttt atcgtaggga ttttcgccag tccgcaccga aagccggatc
      721 ggcacgccct tcagattgaa ggtttcgcga atgccgttcg tgaggaatcg tttgtagctc
      781 tccggcagat gatcaagctg attgccgaag atgacaaaat agggtggccg tgcccgcaat
      841 tgcgtcatgt aacggatttt gatgcggcgt ccggaaaccg ccggcggcgg attggcggcg
      901 agtgcagcag ccagccagcg gttcaggcgt ccggtcgcaa tacgtatgtt ccaggtctca
      961 tgaacggcaa agatcgcgtt catcagcccg tcgatcccct cgcccgtcgc gccggacacc
     1021 gggacgaccg gcatgccctt gatctgcggc agcaggcgtc cggcttcttc gcgaagctcc
     1081 ttcagcctgg cgctgcgatc ctgtaccaga tcccatttat tgatgccaat gacgagcgcc
     1141 ctgccctcgc gtgcgatgag atcagcgaga gtgagatcct gcttttcaaa cggcgcggtc
     1201 gcatcgatca gcagaacgac cacttccgcg aatttcgccg cacgcagacc gtcagcattg
     1261 gcaagcttct cgagcttctc atcgacgcgc gcgcgtttgc gcaggcccgc cgtgtcgaac
     1321 attttgagct tgcgatcgcg ccatacgaga tcgacgccga tggcatcgcg cgtaatcccc
     1381 gcttccgggc cggtcaacaa gcggtcctcg ccgaggatgc gattgagcag cgtcgacttg
     1441 cccgcattcg gccggccgat aatggcgaca cgcaagggct tcgtcggatc gagttcagag
     1501 ccgtcttcct cgtcaccgag cggttctttc gccgcggcgt cgagctcttc ttcacttggt
     1561 tccgcgtctt ccggcagaac ctcacgcagc gcgtcgtaaa gatcactgag accttcgcca
     1621 tgttcggccg aaagcgcgat gggatcgcca aagccgaggt cgtaggcctc ctgcgcgccc
     1681 gcgccgccag cccggccttc ggccttgttg gcgaccagaa tgagcggttt gtcagcgcgg
     1741 cgcagcagcg cggcaaattc gcggtcatcc ggcacaattc cggcgcgcgc atccatcatg
     1801 aacaaaacgg cgtccgcgtc tgcgatggca gctgccgtct gcgcccgcat ccgccctgcg
     1861 agcgttgccg gcgccccggt ctccagcccg gcggtatcga tgatcgtaaa ggtcagatcg
     1921 ccgaggtgag cgacaccctc gcggcgatcg cgcgtcacgc ccggcagatc atcgaccagc
     1981 gcgagctttc tgccgaccag ccggttgaac agcgtcgact tgccgacatt tggacgccca
     2041 acaatggcga tcttgaacat ggctatttcg tcggcacggg gccagcctga acgatcgtca
     2101 ggaacgcgtc ggcgcgcgcc cgcaaggctg gcggcgaaga tgggtcagcc gcggcgagtt
     2161 cgaaccaatg cgcggcgctg gcaaaatccc caaggcgcgt atcggcgagc gccaaaagtt
     2221 cgcgataggc gttgcggtag ggcgagtccg gcccggcata ggccgcgtaa cgctgctcga
     2281 acgccttcgg gtcatcgcta tcgacacgca gataagcgcc gcgcagccgc gcaacctcct
     2341 gataagcggg atcgacagtg ccgttggcgg caacctcgtc ataggctgct gcccccacct
     2401 tgggatcttg cgcggcgagc gcgtcggcgc gggcgagctg cgtcaaggtc gcataacctg
     2461 cgggtgcgtc tgccgcgacc gcagcgaaag cggcggcggc ctgggaattc ttgccggcgc
     2521 tcagcaattg ctccgccgcg gcgtaacggc cgccggccgc ctcagccgac ttgagccgct
     2581 gataatcgaa taaccgccaa ccgccggtgc cgagaacgac cagaacggcc gcgccgatga
     2641 tccagttctg atatcgtttc cagatatcca acaactggtc gcggcggacg tcttcatcta
     2701 cttcgcggaa aaaatcggcc atttatgtct cttctcggct tttacacccc gtgagctagc
     2761 acggcttttc gcgaaacgcg accctcaggg cccctagggc gacagatctg ccatccggtc
     2821 cttggccgca gaaaggccgc cgaccgccgg cacatgctgc gccgcggaag cgaggccaaa
     2881 tctcggcata ttggcgtaga cggtctcata tttacccggc tccgccgcgt aggtctcgtg
     2941 ccagatcccc accgttcccg tttttccgac ctcgcgattg aaggcgaccc aggcgggaaa
     3001 atgcaaagcg tcggtggcgt gggcataggc caccagcttc tcgaagcttt cccaatattg
     3061 ctgaaccaga atgacgcggc cagacaggaa ggttcgttgc gatagcaatc cggcctcggg
     3121 gtgctggccc aattccttca acatgcgcgg catcgccaga aacaccggca gccatttatt
     3181 cacgaggatc agcttgttga atcgcattcc gatcaggaaa agtaccagcg ggcgctccag
     3241 acgcgcggtg taacggccat caaaaatcaa ggccatgaga ttcccctgcg ttcgtgcggc
     3301 aaacacagta tcttactcca agcaagcgat tacggcatcg cggcgcgagg aaatgatatg
     3361 ccccgaaaac cccgcatctt cattggcgtc ggcggctgga cctatgagcc gtggcgcgga
     3421 agcttctatc cggagggtct gccccagaag cgtgagctgg aatatatgag ccgggcgctg
     3481 acctcaatcg agatcaacgg cacctattac ggctcacaga agcccgaaag cttcatcaaa
     3541 tggcgggaag agacgccgga ggatttcgtc ttttcggtca aaggtccgcg tttcgccacc
     3601 aatcggcgcg tgctggccga atccggtgaa tccatcgagc gtttcttcgc gagcggggta
     3661 ctggagctga aagacaagct cggaccaatc aattggcaat ttatggcgac gaaaaaatac
     3721 gacgccgaag atttcgcaaa attcctcgta tcgctgccga aagaaattgg cggccagacg
     3781 atccgccacg ccatcgaagt ccggcacgaa agttttcgca cgccggaatt tgtggcgctc
     3841 gcgcgcgaac atggcgtggc cgtcgttatc gctggcgatt ccgagttccc ccaaatcgcc
     3901 gatgtcacgg cagatttcgt ctatgcgtgg atcatgggga cgaaagaaaa accgaagatc
     3961 ggctatccgg ccaaaacgct cgatcaatgg gccgagcgcg ccgaaatctg ggccgaaggc
     4021 ggcgccccga aagatcttga gaccattacc ccgaaagctg cgaagacacc gcgcgatgtc
     4081 tttctctatg tcatcagcgg gtacaaggaa cgtaatccgc gcgccgcgat ggcactcatc
     4141 gagcgcgccg ggaaaaggat ttgaccgcaa gaaccagagt gcgccctcct acgcgaccta
     4201 ctatttccaa tagcatgcaa acgacagaca acactctggt gcaggacgag cagcgatggg
     4261 ccgctgtgtc tgcgcgcgat catgatttcg acggagactt cgtcttcgcc gtgaagacga
     4321 ccggcattta ctgccgccca tcttgtccgg cacgccatgc acagcggcgc aacgtgaact
     4381 tcttcccgac ctgcgaggcg gctgaacgcg aaggttttcg cgcctgtctg cgctgccgac
     4441 cgcaggaagc acccaaccag cggcaacgcg aagccatcgc cgcagcttgt cgcgcgcttg
     4501 aaaccgatga gccgccgaca ctcgaagtat tggccgcgag agccggatta agtgcgttcc
     4561 atttccaccg tgtcttcaaa gccacgaccg gcgtgacgcc aaaggcttat gcgaaggcgc
     4621 atcgcgccgc gaaagtgcgg caagaactca gtgaggcaac gagtgtgaca gaagccatct
     4681 atgcagccgg ctacaacacc agctccctat tttatgaaaa cgccgacacg atgctcggca
     4741 tggcgccaaa taaattccgc caacagggca gtgatgccaa aatcgtttat ggcatgggca
     4801 agtgttcgct tggtacgatc ctcgtcgcgc gcagtgacaa gggcatttgc gccttgatgc
     4861 ttggtgacga cgctgcagaa ctcgagagcg agatcaagcg gcgcttcgcg aaagcaaagt
     4921 cgatcaaggg cgacgacgcc ttctcggcgc tcctgaccaa cgtcatcgca cttgcggatg
     4981 cgcctgaaaa gaaactcgat ctgccgctcg acattcgcgg cacgctgttt cagcagaagg
     5041 tctgggcagc attgcgcgcc atcccggccg gcgagacggc aagctatacc gagatcgcgc
     5101 ggcgcatcgg atcgccgaca gcaacgcgcg ctgtggcgca ggcatgcgcg gccaacaaga
     5161 tcgctatcgc gatcccctgc catcgtgtcg tccgtagtga tggagcgctg tccggctatc
     5221 gctggggccc ggcgcgcaaa cgggctctgc ttgataagga gcgcaaaaaa taggtcttat
     5281 tcgtcggcgt gcatgacacc cggtcgcgcg gggcgcaggc cctcccgacc gaatacgcgc
     5341 gacaggcttg ccaaagcggc aagcgccgcg agcacggcgg cgatcgtcag cccggccgca
     5401 ctgctttgaa tcgtcgcgcg gaggcaaaga gcggcaatcg tcgccccgat tgtttggccg
     5461 gtaagccgcg cggtcgccaa catgccgccc gcggccccgc tccgatccat cggcgccgag
     5521 gacagcaagg ttcgattatt gggtgcctga aaaaaaccga atcccaggcc gcacagcgac
     5581 atgcgccaaa taatttgaag tgccgaagcc tgaggggaaa gaagagccag tgatgcaagt
     5641 ccgatggcca gaacggtcag ccccacgccg ctgaggatcg ctgacggaaa cttgtcggct
     5701 aggcgccccg caaacggcgc gctgaggccg acagcgactg gccaaggtgt catcagaagg
     5761 ccggtggcga cctgatcgcg atgcagaatt ccttcaaagt aaaacggcag cgacacgaag
     5821 gccagcatct gcgcgctgaa cgatgcgacc gacgtcgtga tcgacaggct gaactcgcgg
     5881 ttcttgagaa gatcgagcgg aatgatgggc gaggtttcgc tacgcgagcg caagaccagt
     5941 gcgaccgcgg cgatcgtcgc cgcgaccacg cagatgatac caaatcggtc ggaggcgccc
     6001 tgcgtccatg cattgatccc gatgaagccg aggccgaaca tcagcacgtt caaaatggca
     6061 ccgagaatat cgagcgagcc cgatctcgga ctttccggca acgacttgcg cccgatcaaa
     6121 aaagccagaa tgccgatcgg cacattcacc gcgaagagcc agttccatga tgcgacagcg
     6181 agtattgccg atgcgatggt gggcccgagc gcggccgccg cagagatgac aagcgcatta
     6241 tacccgactc cgcggccgag caggcgcagc ggataagtat agcgcaccag cgcgccgttc
     6301 acgctcatga tgcaggccgc gccgaggccc tggatcgccc gcgccatggc aagttccggc
     6361 agcgaatgcg caaagctgca accaagcgag gccgcggtga aaaatgcgag accaacctga
     6421 aaaatgttgc gatagccgag catctgccca agcgttgcga gcggcaaaag cgagacgacg
     6481 atcgtcagtt gataggcatt gaccacccaa actgattcgg cagccggcgc cgaaagatca
     6541 cgcgcaattg atggcaatgc gacgttggca atcgaaccat cgagcgccgc gagcgccagc
     6601 gcgagccaga tcgtcgccgc ggaccaatag cgttgcgggg ctggcaaacc gtcgaatgag
     6661 gccaatttta ctccggcgga atcatgtttg gagacgcggg taagcccgcg agcgccgtta
     6721 ccacgaaatc gagaaacgca cgaactttcg ctggcggcag gtgccgcg
//
LOCUS       DASWOG010000338        11566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_282532,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000338 DASWOG010000000
VERSION     DASWOG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282532"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGB93_07035"
     CDS             <1..624
                     /locus_tag="VGB93_07035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0145890.1"
                     /translation="GAYEAVPHGETRGGLVVLQEIFGVNSHIRHVADDFAEQGYHVIA
                     PAIFDRVEPGVDIGYSSDDAAKGVAIRAKIKLEDTLADIAAAVEAASVSGPVGIVGYC
                     WGGTLAFAAAQHVEGLVAAVGYYGSGIAALPKAQLRCPVMLQFGDQDKSIPLADIEKI
                     RREHPDITIHIYPAGHGFSCNDRSAYEPESAKLARERTLEFFEKNIV"
     gene            complement(621..1211)
                     /locus_tag="VGB93_07040"
     CDS             complement(621..1211)
                     /locus_tag="VGB93_07040"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0145891.1"
                     /translation="MAVLPPHAPGLKIGLFGGSFNPAHEGHRAASLLALRRLGLDRVW
                     WLVSPGNPLKDTRGLPSLDQRVAAAARFARDPRIAVTAFEETIGAQYTYQTILYLKAR
                     CRGVHFVWIMGADNLASFEHWKHWRTIAANVPIAIIDRPGSTLSATHARPAQALSRYR
                     HGESDSLTFPLTPPPAMIFLHGPRSELSSTALRSER"
     gene            complement(1232..2524)
                     /locus_tag="VGB93_07045"
     CDS             complement(1232..2524)
                     /locus_tag="VGB93_07045"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY0145892.1"
                     /translation="MDLKLVRNDAPADIGAVMLSLGQAAREASHLLALAPAEAKNKAL
                     LVAAQEISTRAADILAANARDIAEAHAKATNDSFLERLALDAKRVEGMAKGLEEIAAL
                     PDPVGRLLERIERPNGLVIDRVATPLGVIGVIFESRPSVSADAGALCLKSGNAAILRG
                     GSESFHSVSLIHACLQAGLKAADLPAAAITLVPVKDRAAVGEMLTGLGGNIDVIVPRG
                     GRSLVARVEEEARVPVFAHLEGLVHIYVDRDADLAKTLVILRNSKLRRTSVCGAAETL
                     LVDRACAATHLAPLVTMLLDAGCAVRGDAATLAADPRVTPATEDDWSREYLDAIISVR
                     VVDGLDAALAHIAHYGSHHTDCIITENAAAAERFLKEVDSAIVIHNASTQFADGGEFG
                     FGGEIGIATGRMHARGPVGLDQLTTFKYRVHGNGQIRA"
     gene            2647..3876
                     /locus_tag="VGB93_07050"
     CDS             2647..3876
                     /locus_tag="VGB93_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0145893.1"
                     /translation="MTAIALLIFAAIILSGLLSVYLDMRQAAAVRAHRGRVPRPFDAT
                     VTLEEHQRAADYTLARTRLAVIETIYDADLAIVWLAFLLRPVFNFIAAHIPAGLTRSV
                     ATIVLVTAISYLLTLPFSIYKTFRLEAQFGFNRTTPAMFALDQIKAAALQLVLGVPLL
                     YGLFALLHALPSLWWIFAWAALMIIMVAMIVIYPAFIAPLFNKFTPMADGPMKAGIEA
                     LLAKCGFESNGLFVMDASKRSAHGNAYFSGFGRAKRIVFFDTLLEKHTNDEILSVLAH
                     ELGHYKLGHVVQKVGESAVLAFIVFAVLHWAFAAGTLTAAFHLPNDPSLVLMIVMTAI
                     GPFSSLLAPLTNFLSRRAEFQADAFAKSMVGAAPMISALTKLTRDNLATLTPDWLYTL
                     FNYSHPPVPERIAHLQG"
     gene            complement(3873..4202)
                     /locus_tag="VGB93_07055"
     CDS             complement(3873..4202)
                     /locus_tag="VGB93_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018241318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145894.1"
                     /translation="MSNPFQVSLAPQQLWQAINPWTFNQDGAQLGLINIDLAQTPNPQ
                     AERGILDNVGGYGRQIGRIGDALEVILDHLKLKDLTPAEKDAIEILRGQLAQVRQGKA
                     RIEAKSS"
     gene            complement(4265..4762)
                     /locus_tag="VGB93_07060"
     CDS             complement(4265..4762)
                     /locus_tag="VGB93_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145895.1"
                     /translation="MQTFRSFAAALVALASLPAWAAEPQGCGHFKWPLDHEKTLLATP
                     VAITSGDAGKLATGENLTLVPQANAKLPQEPSRPPKFPDSYAGFVTLLAPASAGTYKV
                     TVAHGAWIDVVQDGHVLKATDHTGAVGCDGLTKSVKFDLAATPFTVEISSSTEPSVTL
                     VVTPN"
     gene            complement(4784..5005)
                     /locus_tag="VGB93_07065"
     CDS             complement(4784..5005)
                     /locus_tag="VGB93_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145896.1"
                     /translation="MGWHVRYIDQALKHELLSQEWTTEEEALEDAWNLAQQDGIEITG
                     VEGPDEETVPMEDVQAWFDHRMKQESGPA"
     gene            complement(5077..5817)
                     /gene="aqpZ"
                     /locus_tag="VGB93_07070"
     CDS             complement(5077..5817)
                     /gene="aqpZ"
                     /locus_tag="VGB93_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY0145897.1"
                     /translation="MQNDLVRRVTAAFFGTFWLVFGGCGAAVLAATFPHTGIGFVGVA
                     FAFGLTVLTMAYAVGHISGGHFNPAVTVGLWSAGRVGPDTVVPYIIAQVLGAIAAAAV
                     LHVIASGQAGWVAGGFASNGYGDLSPGKYSLGASALTEFLATFFFLFIIIGSTSKGAA
                     AGFAGIPIGLGLTLIHLITIPVTNTSVNPARSTGPALFAGGAYISQLWLFWLAPLAGA
                     VLAGLLARWLYEPESIADTIVVEERRVA"
     gene            complement(6197..6661)
                     /locus_tag="VGB93_07075"
     CDS             complement(6197..6661)
                     /locus_tag="VGB93_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0145898.1"
                     /translation="MTEGHAAVLEKDFAKPKEHETEFSHVKPGDTEFKGAGLRDFFLY
                     RDLGIAKATKGKVIAHLVKANMAPTTGTGWHKHIADFQIVIMTKGWAKFMYEDKETLV
                     EAGDVVHQRPGITHYLFDYSPDMEYLEIVGPADFGTVEAPAPAEVPAPTPWK"
     gene            complement(6707..7921)
                     /gene="phaZ"
                     /locus_tag="VGB93_07080"
     CDS             complement(6707..7921)
                     /gene="phaZ"
                     /locus_tag="VGB93_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY0145899.1"
                     /translation="MLYRAYQAYSEASEPMRMMARAGLSLGPLFTGFSEATYGKHFTA
                     ALELISRTRLSHARPDYRVESVTIDGEKVPVHEDIAASLPFCNLVRFRKESALPLPKM
                     LLVAPLSGHFATLLRETVRTLVRDHEVYITDWKNARDVPLSEGPFGFDDYVDYVIRCL
                     ELIGPGAHVIAVCQPCVQTLAAVSIMAEQRNPAEPRSMTLMAGPVDVRVNPSKVNELA
                     TSKPMQWFEDNLIAHVPDHLPGGGRRVYPGFVQLTAFVSMNPERHLKAHRDLYEHLSA
                     GRETEAKAIMAFYDEYFAVLDLPAEFYLETVKTVFQDASLAKGELTYRGKKVNPRAIR
                     RTALLTVEGERDDICSVGQTAAAHLLCDGLRPHLKRHHYQVGVGHYGTFSGRRWNGQI
                     YPQIRNLVLAMN"
     gene            8006..8662
                     /locus_tag="VGB93_07085"
     CDS             8006..8662
                     /locus_tag="VGB93_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0145900.1"
                     /translation="MSRPVLTISSKNYSSWSLRGWLLCQLAGLEVEEQVISLDDPSNR
                     AELLLLAPSVLVPRLSEGDVSVWDTLAIAEYLAERNPEAGMLPKDKAARAHCRSISGE
                     IHSGFHNLRSALPMNLKAQHSHFKVFAGAQPDIDRVQTIWQECLTRWGGPFLYGATPT
                     IADAMYAPVATRFLTYGVPLNPAAKAYCATIMDWPPMQAWVADAKAEPEEMAELEVEF
                     "
     gene            9013..10251
                     /locus_tag="VGB93_07090"
     CDS             9013..10251
                     /locus_tag="VGB93_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tlde1 domain-containing protein"
                     /protein_id="HEY0145901.1"
                     /translation="MTYWTYAPQDFASYNNKRGFFRRLLSEAAPGAVVLGLAVFAGAW
                     IVYVHPDAQIKDVEMPAELPTLEAPTLLKPQVAQAVETPSPAPHVAANVYSPFIDPTF
                     SLKAARESFAESAPLAPTFGADPEAASPAQVAAMPTQVASVPLPPVEPQALTAHIPGD
                     EVASAQDETPAKDNVLPMTGDEDLAASAPLPPSSRTVASVPLPAPRPPGLEVPESEDH
                     ARVAVRQAPQPPTQTAEVASEPPSPPPPHNSFFDKLFGGINHQGQALAYAETEDGVVE
                     GTRPSAAVVPYDQYTAVYNISTHTVYLPNGRRLEAHSGLGDAFDDPHYVREKMRGPTP
                     PGIYDLQPREQLFHGVAALRLLPVNGDPYGRVGLLAHRFMLGPRGDSNGCVSFRNYQA
                     FLDAYRAGQVKRLVVVAGMN"
     gene            complement(10268..10651)
                     /locus_tag="VGB93_07095"
     CDS             complement(10268..10651)
                     /locus_tag="VGB93_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145902.1"
                     /translation="MRMSLNAAKAITALDVLVASGFALAGLFHPQAVLPVGDAATPGS
                     AIFAMYAAARTLPLAIFVLIAIFKNWRQTLIVLGILAGTIQFLDFFVGVAQHDAGKSL
                     GPLVLAILQFAAVYALRRDSDHQQV"
     gene            10777..>11566
                     /locus_tag="VGB93_07100"
     CDS             10777..>11566
                     /locus_tag="VGB93_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0145903.1"
                     /translation="MQEQALPAKSTEGRSLRDLASDPRIRAMLGLGFSSGIPFLLVYV
                     TQSAWLSEANVPLGIVGLMSELTLAYKFKFVWAPFLDQYDAPIFSRLLGRRRGWIVVS
                     QIGVALALAGVAFGNPAHWLAWTIVFSLALGFAGATQDIVIDGWCITVAPPERAGLMS
                     SWSEIGWRIGNLVAGAGALYLADAFGWRAAYLCMAACMAPGMIAALMAPEPPSDLEAH
                     IAQGNFVETIVAPIKELLTRLATMGIPILLLVAGFRMPGYVSSAM"
BASE COUNT         2187 a         3614 c         3537 g         2228 t
ORIGIN      
        1 ggtgcctatg aggccgtgcc gcatggcgaa acgcgcggcg gtctcgtcgt cctgcaggaa
       61 attttcggcg tgaatagcca tatccgccac gtcgccgacg atttcgcgga acaaggctac
      121 cacgtcattg cgccggcgat cttcgatcgt gtcgagccgg gcgtcgacat tggctatagc
      181 agcgacgatg ccgccaaagg cgttgccatt cgcgccaaga tcaaattgga ggatacgctc
      241 gctgatatcg ccgcggcggt cgaagccgcg tccgtttcag gccctgtcgg cattgtcggc
      301 tattgctggg gcggcactct cgcgttcgct gcggcccagc atgtcgaggg gctcgtggca
      361 gccgtcggct attacggctc gggcatcgcc gctttgccga aggcgcaatt gcgctgcccg
      421 gtcatgctgc aattcggcga tcaggacaaa agcattccgc tcgcggatat cgaaaagatc
      481 cgtcgcgagc accccgacat cacaatccac atctatcccg ccggtcacgg cttcagctgt
      541 aacgaccgca gcgcttatga gccggagagc gccaagctgg cgcgcgaacg gacgctcgaa
      601 ttcttcgaga aaaatatcgt ctagcgttcg ctgcgcagag ccgtcgagga cagttccgat
      661 cgcggtccgt gcaggaagat catggccggc ggcggcgtga gcggaaaggt cagactgtcg
      721 ctttcaccat gacgatagcg gctgagcgcc tgggctggcc gcgcgtgcgt cgccgagagc
      781 gtcgagccgg gccggtcgat gatcgcaatc gggacattgg cggcaatagt gcgccaatgt
      841 ttccagtgct cgaaactcgc cagattgtcg gcgcccatga tccagacgaa gtgcacgccg
      901 cggcaacgcg ccttcaaata aaggatcgtc tgataggtgt attgcgcgcc gatcgtctct
      961 tcaaaggcgg tgaccgcgat gcgcggatcg cgagcgaatc tcgccgccgc tgcaacacgc
     1021 tgatcgagcg atggcagtcc gcgcgtatcc ttcaacgggt tgccggggct caccagccac
     1081 cagacgcgat cgaggcccag ccgccgcaac gcgagcaggc tcgcggcgcg gtggccctca
     1141 tgcgcaggat tgaaactgcc gccgaacagg ccgattttga gccccggcgc atgcggcggc
     1201 agtacggcca tcgctgacac ggcgggcgct ttcaggctcg aatctggccg ttgccgtgga
     1261 cgcggtattt gaatgtcgtc agttgatcga ggccgaccgg gccgcgcgca tgcatccgtc
     1321 ccgtcgcaat gccgatctcg ccaccgaagc cgaattcgcc gccgtcagcg aattgcgtcg
     1381 aggcattgtg gatgacgatg gcggaatcga cttccttgag gaagcgctct gccgccgccg
     1441 cattttccgt gatgatgcaa tcggtgtgat gcgagccgta atgtgcgata tgcgccagcg
     1501 cggcgtcgag accatcgacg acacgcaccg aaatgattgc atcgagatat tcgcgcgacc
     1561 aatcgtcttc cgtggccggt gtcacgcgtg gatcggcggc gagcgtcgcg gcgtcgccgc
     1621 gtaccgcgca gccggcatcg agcagcatcg tcacaagcgg ggcaagatgc gtcgcggcgc
     1681 aagcccgatc gacgagcagt gtctcggccg cgccgcaaac gctggtacgc cgcagcttgg
     1741 aattgcgcag gatgacgagc gtcttcgcaa gatcggcatc acgatcgaca tagatatgca
     1801 cgagaccttc aagatgcgcg aagaccggaa cgcgcgcctc ttcttcgaca cgcgccacga
     1861 ggcttctgcc gccgcgcggc acgatgacgt cgatattgcc gccaagcccg gtcagcattt
     1921 cgccgacggc ggcgcgatcc ttgaccggta cgagggtgat cgccgccgca ggcaggtccg
     1981 cggctttgag gccggcctgc aggcaggcgt ggatcagcga aacagagtga aagctctccg
     2041 agccgccgcg caggatcgcg gcgttgccgg atttgagaca gagcgcgccg gcatcggccg
     2101 agaccgaggg gcggctctcg aagatgacgc cgatgacgcc gagcggcgtt gccacgcgat
     2161 cgatgacaag cccattcggc cgctcgatcc gttccagaag ccgcccgaca ggatcgggca
     2221 gggcggcgat ctcctcaagc cccttggcca tgccttcgac gcgcttggca tcgagcgcca
     2281 gccgttcgag gaaggaatcg ttggtggcct tggcatgcgc ctcagcgatg tcacgcgcat
     2341 tggcggcgag gatgtcagcc gccctggtgc tgatttcctg agccgcgacc agcagcgcct
     2401 tgttcttggc ctcggctggc gcaagggcga gcaggtgcga ggcctcccgg gcggcctgcc
     2461 caagggacag catcacggcc ccgatgtcgg cgggcgcatc gttgcgtacc agtttgaggt
     2521 ccatatctgt cttcatcggg ctaacttacg tcagtcccac atataaacaa atcgggcgcc
     2581 ttgccagagg cccgggcgat tttggccggc ctcgcatctt ggcttgaaat cgcctaaata
     2641 ggccgcatga cagccattgc cctcttgatt ttcgccgcca tcatcctctc gggcctcctg
     2701 agcgtctatc tcgacatgcg tcaggcggca gcggtccgcg cccatcgcgg cagggtgccc
     2761 cgccccttcg atgccacggt cacgctggag gaacatcagc gcgcagccga ttacaccttg
     2821 gcgcgtacaa ggctcgccgt catcgagacg atatatgatg ccgatctcgc gattgtctgg
     2881 ctcgccttcc tgctgcggcc agttttcaat tttatcgccg cgcatattcc cgcggggctg
     2941 acgcggagcg tcgcgaccat cgttctcgtc acggcgattt cctatctgct caccctgccg
     3001 ttttcgatct acaagacctt ccgcctggag gcgcaattcg gcttcaatcg cacgacgccc
     3061 gcgatgttcg cactcgatca gatcaaggcg gcggcgctgc aactcgttct cggcgtgccg
     3121 ctgctttatg ggctgttcgc gcttttgcac gcgctaccga gcctgtggtg gatttttgcc
     3181 tgggccgcgt tgatgatcat catggtggcg atgatcgtca tctatccggc tttcattgcg
     3241 ccgctgttca acaaattcac gcctatggcc gatggtccga tgaaggccgg cattgaggcg
     3301 ctgcttgcta aatgcggttt cgagtcgaac ggcctcttcg tcatggacgc ctcgaaacgc
     3361 tcggcgcatg gcaatgcgta tttttccggt tttggccgcg ccaagcgcat cgtctttttt
     3421 gacacgctgc ttgagaagca cacgaacgac gaaatattgt ccgtcctcgc gcatgaactc
     3481 gggcattata agcttggtca cgtggtgcag aaggtcggcg agagcgctgt tttggcgttc
     3541 atcgtttttg ctgtcctcca ttgggccttc gcggcgggca cgctaacagc cgcttttcat
     3601 ctgcccaatg atccgagcct ggtgctgatg atcgtgatga ccgcgatcgg tccgttctcg
     3661 agtctcctcg cgccgttgac caacttcctg tcgcgccgcg ctgaattcca ggccgatgct
     3721 ttcgccaaat cgatggttgg cgccgcgccg atgatctcgg ccctgaccaa actcacgcgc
     3781 gacaatctcg cgaccctgac gccggattgg ctttacacgc tcttcaacta ttcgcatccg
     3841 ccggtgccgg agcggattgc gcatctgcaa ggttagctgc tcttcgcttc gatgcgggcc
     3901 ttaccttggc ggacctgcgc gagctggccg cgtaaaatct caatcgcatc cttctcagcg
     3961 ggcgtgagat ccttgagctt caaatgatcg aggatgacct cgagcgcatc gccgatccgg
     4021 ccgatttgcc gcccgtaacc gccgacgttg tcgagaatac cgcgctccgc ctgcgggttc
     4081 ggcgtctggg ccagatcgat gttgatcaaa ccgagctgtg cgccgtcctg attgaaggtc
     4141 cagggattga tggcttgcca aagctgctgt ggcgccagcg agacctgaaa gggattggac
     4201 atgatcgcgc cctgaaagag gcaagcctag ccaggatttc gcggcgcaca aggtcgcgac
     4261 agccctaatt gggtgtgacg acgagcgtca ccgacggctc cgtgctgctg ctgatttcga
     4321 cagtgaaggg cgttgcggcg aggtcgaact tgacgctctt cgtgaggcca tcgcagccaa
     4381 ccgcgccggt gtggtcggtc gctttgagaa catgcccatc ctgaaccaca tcgatccagg
     4441 cgccatgtgc cacggtaacc ttataagtcc cggcgctagc cggcgcgagc aaggtgacaa
     4501 agccggcata ggaatcggga aacttcggcg gccgtgatgg ctcttgcggc agcttcgcat
     4561 tggcttgcgg gacgagcgtc aaattctcac cggtagcgag cttccccgca tcgccggacg
     4621 tgatcgcgac gggcgtggcg agcagcgtct tctcgtggtc gagtggccat ttgaaatggc
     4681 cgcagccctg gggctccgcc gcccaggcgg gtagggaggc aagcgcgaca agcgcggcag
     4741 cgaaactacg aaatgtctgc atggaaattg atgcctcgat ggatcaggcc ggcccggatt
     4801 cttgtttcat gcggtgatcg aaccaggcct gcacatcttc catcggcacc gtttcttcgt
     4861 ccggcccctc aacgcccgtg atttcgattc cgtcctgttg cgcgaggttc cacgcatctt
     4921 ccaacgcttc ttcctcggtc gtccattctt gcgacagaag ctcgtgcttc agcgcctggt
     4981 cgatgtaacg aacgtgccaa cccatatccg cacccttccc ccgatgagac gggcatcacc
     5041 gctttaaacc ggacgatgat gcccgtggtg catatattag gcgacgcggc gctcttccac
     5101 cacaatcgtg tcggcgatgc tctcgggctc atagagccag cgtgccagaa gtccggcgag
     5161 gaccgcgccg gcgagcggcg ccagccagaa cagccagagc tgcgaaatat aggcgccccc
     5221 ggcaaacaac gccggcccgg tgctgcgcgc cgggttgacc gacgtgttgg tgaccgggat
     5281 cgtgatcaaa tggatcagcg tcaggccgag gccgatcggg atgccggcaa agcccgctgc
     5341 tgcgcccttc gatgtcgaac cgatgatgat gaacagaaag aagaaggtcg cgaggaattc
     5401 ggtcagggcg gaggccccca ggctgtattt gccggggctc agatcgccgt aaccgttgga
     5461 cgcgaagccg cccgccaccc agccggcctg acccgacgcg atgacgtgca aaaccgcggc
     5521 agcggcgatc gcgccgagaa cttgcgcgat aatatagggc acgaccgtat cagggccgac
     5581 ccgaccggcg gaccaaaggc cgaccgtcac ggccggattg aaatggccgc cggaaatgtg
     5641 gccgacggca taagccatgg tcagaaccgt gagaccgaag gcgaaagcga caccgacgaa
     5701 gccgatgccg gtgtgcggaa aagttgcggc aaggacggct gcgccgcagc cgccgaagac
     5761 gagccaaaac gtgccgaaga atgcggcggt gacgcgccgg acgagatcgt tttgcataag
     5821 ctacctcgtg gaatcgctgg agacattgta tctctgccga atttgcgaat ccggttctga
     5881 gacgggggca gtttcttatg acaaacacgc gaggcgcaca gactcgacct cgcggcgcgg
     5941 agaatgcttc gcacccaggc tcaagtcgag tcacagacgc ttcaagacgc agtcaagctc
     6001 aagtccggcg ctgtcaaaaa ttgcgttggg gaagctattc gccgctctga gcggcggcgc
     6061 aatgcggcgc gaggaatgcc gcagaaaaaa cctgtggaat ctgtcacagc cgtgattcac
     6121 aggcacattc gacaaggccg gccgggaggg ccggccggct tcaaaaagat cggcgacgat
     6181 cgcgaagcgc cgacgcctat ttccaagggg tcggcgccgg cacctcggca ggggcaggcg
     6241 cctcgaccgt gccgaaatca gccgggccaa cgatttcgag atattccatg tccggcgaat
     6301 aatcgaacag gtaatgcgtg atgcccggac gctggtgcac gacatcgccg gcctcgacga
     6361 gcgtttcctt gtcctcgtac atgaatttcg cccagccctt cgtcatgatg acgatctgga
     6421 aatcggcgat gtgtttatgc cagccggtgc cggtggtcgg cgccatattg gccttgacga
     6481 gatgggcgat cactttgccc tttgtcgcct tggcgatgcc gaggtcgcga tacagaaaaa
     6541 agtcgcgcag gccggcgccc ttgaactcag tgtcgccggg tttgacatgc gagaattcag
     6601 tctcgtgctc tttcggcttc gcgaaatctt tttctaaaac ggcggcatgt ccttctgtca
     6661 tctctaacct cctgcgttca gcatgtccgc ggcccgctgg gccaactcaa ttcattgcaa
     6721 gaaccagatt ccggatttgc gggtaaatct gtccgttcca gcgccggccg ctgaatgtgc
     6781 cgtagtgacc gacgccgacc tggtaatgat gacgcttcaa atgcgggcgc agcccgtcac
     6841 agagcaaatg cgcggccgcc gtttggccga cagagcagat atcgtcgcgc tcgccctcaa
     6901 cggtaagaag cgccgtgcgg cgaatggcgc gcgggttcac tttctttccg cgataggtta
     6961 actcgccttt tgccagcgag gcgtcctgaa acacggtctt gaccgtctcg agatagaatt
     7021 ctgccggcag atcgagcacg gcgaaatatt cgtcatagaa cgccatgatc gccttggctt
     7081 cagtctcgcg cccggcgctg agatgttcgt agaggtcgcg atgtgccttg agatggcgtt
     7141 ccgggttcat cgacacgaac gcggtcaatt gcacgaaacc gggatagacc cgccgtccgc
     7201 cgccgggcag atggtccggg acatgcgcga tcagattgtc ttcgaaccat tgcatgggct
     7261 tcgacgtcgc aagctcgtta accttcgacg gattgacacg cacgtcgacc gggccggcca
     7321 tcagcgtcat gctgcgcggc tcggcggggt tgcgctgttc ggccataatc gagacggcgg
     7381 caagggtctg aacgcaaggc tgacagacgg cgatgacatg ggcgccgggg ccgatcagct
     7441 ccagacagcg gatgacgtaa tcgacataat cgtcgaagcc gaagggcccc tccgataagg
     7501 gcacgtcacg ggcgttcttc cagtcggtga tatagacctc gtggtcgcgc accagggtgc
     7561 gcaccgtctc acgcagcagc gtcgcgaaat ggccggagag gggcgcgacg agcagcattt
     7621 tcggcagcgg caaggcgctc tctttacgaa agcgcacgag gttgcagaag gggagcgagg
     7681 cggcgatatc ctcgtgaacc ggcaccttct cgccgtcgat cgtcacgctc tcgacgcgat
     7741 agtccggccg ggcgtgcgac aatctggtgc gggaaatcaa ttccagcgcc gcggtgaagt
     7801 gtttgccata agtggcttca ctgaagccgg taaacagcgg gccaaggctc aagcccgcac
     7861 gcgccatcat ccgcatgggc tcagaggctt ccgaataagc ctggtatgcg cggtacaaca
     7921 tgtgcttcaa ggctaaagtc tttcgttttg cgccgcaata tcggcgtgtg cttaaagttt
     7981 cacccgttcg cgtccaggag cggtcatgtc gcgtcccgtt ctcaccatct caagcaaaaa
     8041 ctactcctct tggtccctcc gcggctggct gctgtgccaa ctcgccggat tagaggttga
     8101 agagcaggtc atcagcctcg acgatccgtc gaaccgggcc gaattgctgc tgctggcgcc
     8161 gtcggtgctg gtgccgcggc tgtccgaggg cgacgtcagc gtgtgggaca cgttggcgat
     8221 cgccgaatat ctcgcggagc gaaacccgga agcgggcatg ttgcccaaag ataaggcggc
     8281 gcgcgcgcat tgccgttcga tctcgggtga aatccactcc ggctttcata atcttcgctc
     8341 ggcgctgccg atgaacctga aagcccaaca ctcgcatttc aaagtctttg ccggcgcgca
     8401 gccggacatc gaccgcgtgc aaacgatctg gcaggaatgc ctgacgcggt ggggtggccc
     8461 atttctctat ggcgcgacgc cgaccatcgc ggacgcgatg tacgcgcccg tcgcgacgcg
     8521 atttcttacc tatggggtgc cgctcaaccc ggccgccaaa gcctattgcg cgacgatcat
     8581 ggattggccg ccgatgcagg cctgggtcgc cgacgccaag gccgaacccg aggaaatggc
     8641 cgaactcgaa gtcgaattct gacttcgagc ccccctcatc gattgttgca tcaaagccgc
     8701 ggcggggccg gattccgtcg cgcgacccac ggattctgcc gttccgggct tgtccacgcg
     8761 attttttttt ggaaggctgc cgtggagagc gagggcaaga ataatcaacg aaaataatcg
     8821 ccagccttag gaccgcaggg gaattgccct gccaatgccg caatcggcac tattctgaag
     8881 agaaggtcat taatcggcat ttaactaaaa acgtcctcaa ataaaggcct cggcatctgg
     8941 gcaacacctc aaggtggtca agccccggca agccagaagg cgtaaatcta gtcgcacagg
     9001 acgcggggtg ggatgacata ctggacttac gcgccgcagg attttgcctc gtacaataat
     9061 aaacgaggtt tcttccgcag acttctttcc gaggccgcgc ccggcgcggt tgttcttgga
     9121 ctcgcggttt ttgccggcgc ctggatcgtc tacgtacacc ccgatgcgca gatcaaagac
     9181 gtcgagatgc cggccgaact gccgactctc gaagctccta cactcctcaa gccgcaagtc
     9241 gcgcaggccg tcgagacacc atctccggcc ccgcatgtcg cagcgaatgt ctatagcccc
     9301 ttcatcgacc cgaccttttc gctgaaagca gcgcgggaat cttttgccga gagcgcgccg
     9361 ctggcgccga ccttcggtgc cgatccggaa gccgcgtcgc cggcacaggt cgccgccatg
     9421 ccgacgcagg tcgcctcggt tccgctgccc cccgtcgagc cgcaagccct gaccgcgcac
     9481 attccgggcg atgaagtcgc ctcggcgcaa gatgaaaccc cggcgaaaga caacgtcctg
     9541 ccgatgaccg gggacgagga tctcgccgcc agcgcaccgc tgccgccctc gtcgcgtact
     9601 gtcgcgagtg tgccgctgcc ggcaccgcgc ccgcccggtc ttgaagtgcc cgaatccgaa
     9661 gatcacgccc gcgtcgccgt ccgtcaggcg ccgcagccgc cgacgcaaac ggccgaagtc
     9721 gcgtccgagc cgccgtcacc gcccccgcca cacaattcct tcttcgacaa gctcttcggt
     9781 ggcatcaacc atcaaggaca ggcgcttgcc tatgccgaaa cggaagatgg cgtcgtcgaa
     9841 ggcacgcgcc cgtccgcggc ggtcgttcct tacgatcaat acacggccgt ctataatatc
     9901 tcgacccaca cagtttattt gccgaacggc cgccgccttg aggcgcattc gggcctcggc
     9961 gacgccttcg atgatccgca ttacgtccgc gagaaaatgc gcggaccgac accgcccggc
    10021 atctatgatc tgcagccgcg cgaacaactc ttccacggtg tcgcggcgct gcgtctgctg
    10081 ccggttaatg gtgatcctta tggccgcgtc ggtctcctcg ctcaccggtt catgcttggc
    10141 ccgcgtggcg attccaacgg ctgcgtctcc ttccgcaatt accaagcctt cctcgacgcc
    10201 tatcgcgccg gccaggtcaa gcgcctggtt gttgtcgccg ggatgaactg agcggccagc
    10261 cgccttatca aacctgctga tgatcggaat cgcggcgcaa agcgtaaacc gccgcgaatt
    10321 gcaggattgc gagtacaagc ggaccaagcg actttcccgc gtcgtgctgg gcaacgccaa
    10381 cgaaaaaatc cagaaactgg attgtgccgg caaggatgcc gaggacgatc aatgtctgcc
    10441 gccagttttt gaaaatcgcg atcagcacga agatggcaag cggaagcgtc cgcgccgcgg
    10501 catacatggc aaagatcgcc gagcccggcg tcgccgcatc gcctaccggt agaaccgctt
    10561 gcggatgaaa gaggccggca agtgcaaagc cgctcgcaac cagcacatcg agcgcggtga
    10621 tcgccttggc agcgtttaga gacatacgca tcgttaacct tcgtcctcag acatccctgc
    10681 ccgctggccc tgcgacgatc taggttctcc gccaccgata ggcaaatgtc agaaagacag
    10741 gctaatccct gctggggaaa ccgatctgga atgtccatgc aagagcaagc tttgcccgcc
    10801 aaatcgaccg agggacgatc gctgcgcgat cttgccagcg acccgcgcat ccgcgccatg
    10861 ctcgggctcg gtttcagctc cggcatcccg tttctgctcg tctatgttac acaatcggca
    10921 tggctgtcgg aagccaacgt gccgctcggc attgtcggcc tgatgagcga actgacgctc
    10981 gcttataaat tcaaattcgt ctgggcgccg tttctcgatc aatacgacgc gccgatcttc
    11041 agccgccttc ttggccgccg gcgcggctgg atcgtcgttt cgcagatcgg cgtcgctctg
    11101 gcgctcgctg gtgtcgcctt cggcaatccg gcgcattggc tcgcctggac aatcgtgttc
    11161 tcgctggcgt tgggctttgc cggcgcgacg caggatatcg ttatcgacgg ctggtgtatc
    11221 acggtcgcgc cgcccgagcg cgccggcctc atgtcgtcat ggtcggagat cggctggcgc
    11281 atcggcaatc tcgtcgccgg tgccggcgcg ctctacctcg ccgatgcctt tggctggcgc
    11341 gcggcttatc tctgcatggc ggcgtgcatg gcgccgggca tgatcgctgc gctcatggcg
    11401 cccgagccgc cgtccgatct cgaagcgcat attgcacagg gcaatttcgt cgagacgatt
    11461 gttgcgccga tcaaggaatt gctgacgcgt ctcgccacga tgggcattcc gatcctgcta
    11521 ctcgtcgccg ggttccgcat gcccggctat gtgtcgagcg cgatgg
//
LOCUS       DASWOG010000339         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_117761,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000339 DASWOG010000000
VERSION     DASWOG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117761"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGB93_07105"
     CDS             <1..891
                     /locus_tag="VGB93_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0145904.1"
                     /translation="ILVDALEKIGVKHIFGLISDSLNPIGDVVRQSKIEWVGVRHEEG
                     GALAASGQAKLTGRLGVCCGSTGPGSTHLIAGLYEASRDHAPVLAISGDMPRKLEGVD
                     YFQATNPNTNFGDVSIYTETISSPEQAPAVIHQAIAAAYAGRGVAHLTIPVDVLTAKA
                     GGGVSSIATLKPRPEIVASEDDIAEIARRIDEAGRIVIMCGAGCHGAADELRALSDRL
                     KAPLIHSVKGKDIMPYDDPHWMGGIGMIGTKPVYKAVMDCDLFLMLGTDYPYSAFLPP
                     KGVVIQVDERARVLGRRAPT"
     gene            910..1671
                     /locus_tag="VGB93_07110"
     CDS             910..1671
                     /locus_tag="VGB93_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0145905.1"
                     /translation="MRPTVRSLLGRVKPKSDGFFFDSLATRRKAWDEMLDKQSDPARS
                     KDCIHPQAVARAVSDLAARDAVFVIDTGLNTLWSGNWIRQSGQQRIIGSFNNGAVGTA
                     LGQANGIQALDRSRQVIALCGDGGFNMLMSEFLTAVHHKLPVKAVVYNNSSLGLIKLE
                     AESMGLPAWKAMDFPNPDYVTLARACGAVGFKAEKPGELRDAISAGLKADGPAIIDCV
                     VAADELPNAPHLELETIGNVAKAKIKEAILNVTGG"
     gene            complement(1795..2688)
                     /locus_tag="VGB93_07115"
     CDS             complement(1795..2688)
                     /locus_tag="VGB93_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEY0145906.1"
                     /translation="MKKTTLFKQYLLAQDILLIPVAHDPLGAKIIERAGFKVLGCGGY
                     ANSAAFMGVPDIEVLTLTNMVDCVWRMCDAVDLPVWVDGDTGHGNVTNVQHTVRQMEK
                     AGAVSIMLEDQVSPKRCGHMSGKHVVPADEFIAKIKAGVDARRDEDFTVLARTDAIGV
                     HGLDEAVDRAHRAVEAGADWVFLEAPENLDQMRQIPKLVSVPTLANMIPGGKTPIVPA
                     AELQSFGFAAVVWPNAFTYAYAKLATELAKGLLSTGTTASFDERMIKFDEFNELVGLP
                     KIRENEERYYAPFEDADIKPV"
     gene            3016..3603
                     /locus_tag="VGB93_07120"
     CDS             3016..3603
                     /locus_tag="VGB93_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145907.1"
                     /translation="MPIRGDVIHTVNFFQGAFTIILALALGEALKSFASEDQSQSLHW
                     ERLPALLTFMLIFFQFFESMAEYFYSTYLSPKTALEFVPGYLIFDGIMFMAEGCCFFI
                     MSRSLAPQRWQRFYGGVLVLMLIDIVWTGINQIRGLHVGPWLVIDITIVVAILLMMWF
                     ERGKDESMRPGYIGLALIAIAAVLGYWLEHDMYFP"
     gene            3600..4583
                     /locus_tag="VGB93_07125"
     CDS             3600..4583
                     /locus_tag="VGB93_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0145908.1"
                     /translation="MNTTHDLCKIALSIACLALGVVSLSAGYPATTLASDLRLPPQTI
                     CATPPITSPIYADVHEQIAQTSRGPMAYYRFGRGSPVLLVTGFRATLSEWNAQFLSQL
                     AKRNEVAVFDNRGVGRSIPDASGFSVRDMANDTASLIQALKLRRPTVVGWSMGGVVVQ
                     QLAIDDPASVGQMVLLSTLAPGKSGIPIRPRIEAVLSGAPSVTFDQVMSVLFPPASLF
                     TAKRCFRQEMYEPRDYGSPVISNAVTNGQSALLKAWSHDESAAKAAPAVRLRTLILSG
                     SDDDVVSVRNSAALRNLLPNARLVTIGRAGHAMMYQYPVVLAQKISAFAKP"
     gene            complement(4586..>5326)
                     /locus_tag="VGB93_07130"
     CDS             complement(4586..>5326)
                     /locus_tag="VGB93_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY0145909.1"
                     /translation="AEVAKAAGVTPEHIRHRAEELQEFNPMLGFRGCRLAIAFPEIAE
                     MQARAIFEGAVDAKRKTGQAPEPEIMVPLVAIKPELDAVKAIIDKVAEAVAAETGETI
                     AYSVGTMIELPRAALKAGEIAETAEFFSFGTNDLTQTALGISRDDAGTFLGIYISKGL
                     LARDPFATIDQQGVGELVEIAVERGRKTRPDIKLGICGEHGGDPASIGFFERAGLDYV
                     SWSPFRVPIARLAAAQAALGGLRSGEDS"
BASE COUNT         1031 a         1614 c         1549 g         1132 t
ORIGIN      
        1 atcttggttg acgcgctcga gaagattggc gtcaagcaca tcttcggcct cattagcgat
       61 tcactcaatc cgatcggcga cgtcgtgcgc cagagcaaaa ttgaatgggt cggcgtccgc
      121 catgaggagg ggggcgcgct cgcggcatcc ggccaggcaa agctcaccgg tcggcttggc
      181 gtctgttgcg gatcaaccgg ccccggcagc acccatctaa tcgccggcct ttatgaggcg
      241 agccgcgacc atgcgccggt tcttgcaatc tccggtgata tgccacgcaa gctggagggg
      301 gtcgactatt tccaggcgac aaatcccaac acgaacttcg gtgatgtctc gatctatacc
      361 gagaccattt cgtcgccaga acaggcgcca gcggtcattc atcaagccat tgctgccgcc
      421 tatgcgggcc gtggcgttgc gcatttgacg ataccggttg acgtcctcac ggcaaaggcc
      481 ggcggcggtg tttcgagcat cgccacgctc aagccgcggc ccgaaatcgt cgccagcgaa
      541 gacgacattg ccgagatcgc ccggcgcatc gacgaagccg gtcgcatcgt catcatgtgc
      601 ggtgcgggat gtcatggtgc agccgacgaa ttgcgggcgc tctctgatcg gctcaaggcg
      661 ccattaatcc attccgttaa aggcaaagac atcatgcctt acgacgatcc gcactggatg
      721 ggcggcatag ggatgatcgg aacgaagccg gtttataagg ccgtcatgga ttgcgatctg
      781 tttctgatgc tcggcacgga ctatccatac tccgcgttct tgccgcctaa gggtgtcgtc
      841 attcaggtcg atgagcgcgc gcgtgttctc ggccgccgcg cgccgactta actcggcgtg
      901 atcggctcgg tgcgaccgac ggttcgctcg ctgcttggtc gggtgaagcc gaagagcgac
      961 ggcttcttct tcgatagcct cgcaacccgg cgcaaggcgt gggacgagat gttggataag
     1021 cagtccgacc ccgcgcgcag caaagattgt attcatccgc aagccgtggc gcgcgcggtg
     1081 agcgaccttg ccgcgcgcga cgccgtgttc gtcatcgaca ccgggcttaa cacgctctgg
     1141 tcgggcaact ggatccgcca gagcggccag cagcggatta taggctcgtt caacaatggc
     1201 gccgtcggca ccgcccttgg ccaggccaat ggcattcagg cgcttgaccg ttcgcgccaa
     1261 gttatcgcgc tctgcggcga cggtggtttc aacatgctga tgtccgaatt cctcaccgcc
     1321 gtgcaccata agctcccggt gaaagccgtg gtctacaaca attcgtccct cggactgatc
     1381 aagctggagg ccgagtcgat ggggctgccg gcctggaagg ccatggactt ccccaatccg
     1441 gactatgtga cgctggcgcg cgcctgcggt gccgtgggct tcaaggcgga aaagcccggc
     1501 gaattacgcg atgccataag cgccggactc aaagccgatg gcccggccat tatcgattgc
     1561 gtggtcgcag ccgacgaatt gcccaacgcc ccacatctcg agctcgagac aataggcaat
     1621 gtcgcaaaag caaaaatcaa ggaagcgata cttaatgtca ctggcggatg acggagcgcc
     1681 gcgacagcgt cgtcgtcaag acccgaggaa atgggtttgt attcggcacg aggcggcggc
     1741 ggccatcccc ctccgcagca agcgtcatgg ccgtttcaaa tccgatggaa agactcagac
     1801 tggcttgatg tccgcatctt cgaaaggcgc gtagtagcgc tcctcgttct cccggatttt
     1861 cggcaatccg accaattcgt tgaattcgtc aaacttgatc atccgctcgt caaacgacgc
     1921 cgtcgtgccg gtgctgagca gccccttggc aagctcggtc gcaagcttgg cgtaggcata
     1981 ggtaaacgca ttcggccaga cgacggcggc aaaaccgaac gattgcagct cggccgctgg
     2041 cacgatcggc gtcttaccgc ccggaatcat gttggcaagc gtagggacag acacgagctt
     2101 cgggatctgg cgcatttgat cgaggttctc gggcgcttcg agaaaaaccc aatcggcgcc
     2161 ggcttcgacg gcccgatggg cacggtcgac ggcctcgtcc agaccatgga cgccgatggc
     2221 atcggtgcgc gccagaacgg tgaagtcctc atccctgcgc gcgtcgacgc cggctttaat
     2281 cttggcgatg aactcatcgg ccggcaccac atgcttacct gacatatggc cgcaacgctt
     2341 cggcgagacc tggtcttcga gcatgatgga caccgcgccg gccttttcca tttgcctgac
     2401 cgtatgttgg acgtttgtca cattgccatg tccggtgtcg ccatcgaccc agaccggaag
     2461 atcaaccgcg tcgcacatcc gccagacgca atcgaccatg ttcgtgagcg tcagcacttc
     2521 gatatccggg acgcccatga aagccgcgga gttggcatag cccccgcaac ccagcacctt
     2581 gaagcctgcc cgctcaatta ttttcgcgcc gagcggatcg tgggcgaccg ggatcagcaa
     2641 tatgtcctgg gccaacagat attgtttgaa cagggttgtt ttcttcatga cgccgatttc
     2701 ctatcggtcg gatggggggc aatttccgac gcgaaatggc ggactcttcg agtccgtttc
     2761 ggcatgcggt tcctgtcaga atactacccg tcgtgattcc tctcaattct cgacggcaat
     2821 taagtttgcg ctatcgccca atcggataac gcatggtcaa caccggcgat atgttcaagc
     2881 agtggcggag acagaccggc agcatcgacg ccatcaccgc agacgatgac ataatcggcc
     2941 tttcctttgt atgggtggcg aattacgttt tgcgctccct cttcctcccc gctccatgtc
     3001 ttcgggaaca acgcaatgcc gattcgcggc gacgtcattc atacggtcaa tttcttccaa
     3061 ggcgctttca ctatcattct ggcgctggcc ctcggcgaag cgctgaaatc tttcgccagc
     3121 gaagaccaga gtcaatcgct gcactgggag cgcctcccgg ccttgctgac tttcatgctc
     3181 atcttcttcc agttctttga gagcatggcc gaatattttt actccacata tctcagtccg
     3241 aagacagcgc tggaatttgt tcccggatat ctcatcttcg acggtatcat gttcatggcg
     3301 gagggctgtt gctttttcat aatgtcgcgg tccctggccc cccagcgctg gcagcgcttc
     3361 tacggcggcg tgctcgtgct catgctgatc gatattgtct ggaccggcat caaccaaatc
     3421 cgtggcctgc atgtaggtcc ttggctggtc atcgacatca ccatcgtcgt cgcgattttg
     3481 ctcatgatgt ggttcgagcg cggcaaggat gagtcgatgc gaccgggcta tatcgggctt
     3541 gcgcttatcg cgatcgccgc ggtacttggc tattggttgg agcacgacat gtattttccg
     3601 tgaacacgac gcacgatctc tgcaaaatcg ccctttcaat cgcctgtctg gcgctcgggg
     3661 tcgtgagtct atctgcgggc tatcccgcaa cgacgcttgc ttcggatctg cggcttccgc
     3721 cccaaactat ctgcgccacg ccacccatta catcgccgat ctacgccgat gtgcatgagc
     3781 agatcgcgca aacatcgcgc gggccaatgg cctattatcg gttcggtcgt ggcagtccgg
     3841 tcctgctcgt caccggattc cgtgcgacgt tgtccgagtg gaacgcccag ttcctatctc
     3901 agctagcaaa acgcaacgaa gtcgccgtct tcgacaatcg tggcgtggga cgatcaattc
     3961 ccgacgcgtc cggcttcagt gtgagggaca tggcgaatga tacggcttcg ctgatccaag
     4021 cgctgaaatt gcggcgtccc accgtggtcg gttggtccat gggaggcgtc gtcgtacagc
     4081 aactcgcgat cgacgatcca gcgtcggttg gtcaaatggt actcttgagc accctcgcgc
     4141 caggcaaatc cggaattccc attcggcctc gcatcgaggc agtgctgtcg ggcgcgccga
     4201 gcgtcacatt tgaccaagtc atgagcgtac tttttccgcc ggcgagtctc tttacagcca
     4261 agcggtgctt ccggcaggaa atgtacgagc cgcgcgatta tggctctccg gtcatttcaa
     4321 acgcggtcac gaatggacaa tcggctctgt tgaaggcttg gtcgcatgac gaaagcgcgg
     4381 cgaaagcggc tccggcagtg agacttagaa ctctgattct ttccggatca gacgatgacg
     4441 tcgtctccgt caggaattcc gcggcgcttc ggaatcttct gccaaatgcg cgtttagtca
     4501 cgattggccg agccggtcat gctatgatgt atcaatatcc tgtggtgctc gcacagaaga
     4561 ttagcgcctt cgcgaagcca tagatttacg aatcctcgcc gctgcgtaga ccgccgagtg
     4621 cggcctgcgc ggcggcgagc cgggcgatgg gtacgcgaaa cggcgaccag gatacgtaat
     4681 cgagccctgc cctttcgaag aagccgatcg aggcaggatc gccgccgtgt tcgccgcaaa
     4741 tcccgagctt gatgtcgggc ctggtcttgc gaccgcgttc gaccgcgatc tcaaccaatt
     4801 cgccgacgcc ttgctgatcg atcgtggcga aaggatcgcg cgcaagcagg cctttcgaga
     4861 tgtagatgcc gaggaatgtg ccggcatcgt cgcgcgaaat gcccagcgcc gtctgcgtga
     4921 gatcgttggt gccgaatgag aagaattccg ccgtttcggc aatctcgcca gccttgagcg
     4981 ccgcgcgcgg caattcgatc atcgtcccga cgctataagc gatcgtttcg ccggtttcgg
     5041 ccgcgacggc ctcagcgacc ttatcgatga tcgccttcac cgcatcgagt tcaggcttga
     5101 tcgccacgag cggcaccatg atctcgggct cgggcgcctg ccccgtcttg cgcttggcat
     5161 cgaccgcacc ctcgaaaatg gcgcgcgcct gcatttcggc gatttcggga aacgcgatcg
     5221 ccagacggca gccgcggaag cccagcatcg gattgaattc ctgcaattcc tctgcacgat
     5281 gccggatatg ttcaggcgtg acgcccgcgg ctttcgcgac ctcggc
//
LOCUS       DASWOG010000340         6208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_209186,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000340 DASWOG010000000
VERSION     DASWOG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209186"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGB93_07135"
     CDS             complement(<1..435)
                     /locus_tag="VGB93_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145910.1"
                     /translation="MAEVSFHNVRKSFGGPRQKSVDVLADLDFSVNDGEIVALCGPSG
                     CGKTTALRIAMGLEKATQGHVCVDGRPVQGCGFDRGIIFQHAELLPWLTARANVLFGL
                     ELKRVPKEEAQEIVERCLQLVGLKGSAERRPHQLSGGMKQRVG"
     gene            complement(450..1421)
                     /locus_tag="VGB93_07140"
     CDS             complement(450..1421)
                     /locus_tag="VGB93_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0145911.1"
                     /translation="MGDNYPTVRLAAVQAAPVWLDRDATVSKACRLIEEAADHGAKII
                     GFPEGFLPGFPDWFHWFMPRSAESIAHSKSLFKNAIEVPSAAIDTIGAAAKRSKVYVV
                     AGASERDVGTMGTLYNSLLFFGPEGDLLGVHRKLMPTMTERLVHASGDGSSMRTYETP
                     YGNIGGLICGENTNSLARFSLLAQNERIHIASWPAFPAGKRNFDAIDIRAKYHAFEGR
                     VFVISACGIFDDACLDAMGLTAEQKAAMPCRGGHSGILAPDGSYIAGPVDDTPQIVYG
                     DADLEKVLEGKLSHDLTGHYNRFDVFTLQVHTARRKALERVTPDSGS"
     gene            complement(1526..2500)
                     /locus_tag="VGB93_07145"
     CDS             complement(1526..2500)
                     /locus_tag="VGB93_07145"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0145912.1"
                     /translation="MIRKISYALLLLLGAAGTADAETATWRHAIVEPKSDSGIAMMAG
                     KSDIAGRHGLNIEYVPVKGDALALKALIAGDVDSYEGTPGGAIIAASRGANIKLVGCY
                     WPGLSYGIFSTNKIATIDQLKNGTFAISNPGSLPDLISRGILRKHNIPLSGVRFASLG
                     GDADRFRALSQGLVDAAAISTEFVPFAADHQVHLLFDAHQELPNFLRWCTYVSASKIA
                     QDRAEVVSFVAAQMDGVRFAMEHKQASIDLTVKTLNIKLNDPRPAYIYDQALKLNLLD
                     PTMNVPMEKLGWMKQLLIDTGNLSAPVDIENFVDTSIRTEALSHRLIK"
     gene            complement(2497..4173)
                     /locus_tag="VGB93_07150"
     CDS             complement(2497..4173)
                     /locus_tag="VGB93_07150"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY0145913.1"
                     /translation="MAISDDLKSKLSCYARETGATHLYPDLHDHVRNLADAGLLFVVE
                     EPVNKDTIMHPLVRWQYRGSIPEAERRAFLFLNPTDSKGRKYDGAVLVAGLAGSREIY
                     RVGFGADLDAIRDRWLKALATPLAPVVVEHAACHDIVTLHDELDQPGRGLDSLPVPIS
                     TPGWDNAPYLTAGHYITRDPDTGIQNVGNYRGQIKAPRRIGMNPSVEIRPGILVHWEK
                     HKARGERLPCAIVLGCPPAVSYAAVQKMPESLDEVWVAGALTGTPIEVVRAKTVDLLV
                     PAQAEIVIEGFIETVMREPEAPFGESHGYVHLQEYNAYMEVTAITRRRNPILPSFISQ
                     VTPSESSVIRRAAMEPVLFNHLRSTMGVTGVKRVHMHEPMTSVIALFIIQFARNTLET
                     EIWRALYGAASLHRYAGKWIVAVDEDIDPENADAIFWAMCYRCQPQNDLKILDKKDPG
                     HGPRNETNSAGSASVLINATMRGDFPPIALPKREFMEEARELWNKLGLRPLQPESPWF
                     GYDLGNWPDWLEEQAKLATQSEYFKTGERIAKQRRSDVEMNAPISGNPDT"
     gene            4558..6141
                     /locus_tag="VGB93_07155"
     CDS             4558..6141
                     /locus_tag="VGB93_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0145914.1"
                     /translation="MMAARKRFVWRVAGLALSCIAGFSASDLVAAPDQTGIVHTDAGN
                     VRGLINGDVRRYLGIPYAAPPIGALRWRPPVNARPWVTVLDATKFGGTCAQAQRGVFA
                     APSQNEDCLYLNIYTPRETNAAARNPVMVWFNGGGLFSGESNDYDGSKLVARGNVIVV
                     TLNYRVGVFGFFSHPALNAEGHPYANYGIMDQQFALKWVQRNISAFGGDPNNVTIFGQ
                     SGGGTSVMANLVSPLSKGLFQKAINESGTSIQVVTPAASLDDGVQLANSVGCAGADSA
                     KCMRALSVKQILDHQAPILRHVVVFPAIDGTVITKTALDAFSHGSFNHVPIMTGLVAD
                     EQSFFMSELKTHKPALPAEYEALVASFGHQNRDKIAARYPLNKYPSPALAEVAAAQGF
                     KSCTARLLDRAWAKYAPVYAYEFRDQTAPSYFPKFSFPMRAYHTAELQYIFPRFHGGR
                     GTPHALNAAQEKLSDQMVDYWTSFARTGRPAISASPPWKPYDAQTDNYQVLDLSGVSN
                     VGGYGKTYDCELWDAILSL"
BASE COUNT         1363 a         1791 c         1710 g         1344 t
ORIGIN      
        1 gcctacccgc tgtttcatac caccggaaag ctgatgcggg cgccgctccg ccgaaccttt
       61 caagccgacc aattgcagac aacgttcgac gatctcctgc gcctcctctt tggggacgcg
      121 cttcagctca agtccgaaaa gcacgttcgc gcgtgccgtc agccagggga gcaattcggc
      181 gtgctggaag atgataccgc gatcaaatcc gcagccttgc accggtcgcc catcgacaca
      241 tacatgacct tgcgtcgcct tctccaaccc catcgcaatc cgaagcgcag tcgtcttgcc
      301 gcagccactt ggcccgcaga gcgcgacgat ctcaccatca tttaccgaga agtcgagatc
      361 agccaggaca tcgaccgatt tctgacgcgg cccaccgaaa gacttccgaa cgttatgaaa
      421 tgaaacctca gccatcggcg cccttcgttc taactaccgg aatcaggagt gactcgttct
      481 aatgctttgc gacgcgccgt gtggacctgc agagtgaaga catcgaagcg gttataatgg
      541 ccggtcagat catgactgag cttgccttcg agcactttct caagatccgc atcgccatag
      601 acgatctgcg gcgtatcatc caccgggccc gcgatataag aaccatctgg cgctaaaatg
      661 ccggaatggc ctccccggca cggcattgcg gctttctgtt ccgccgtcag acccattgcg
      721 tctaggcagg catcgtcaaa aatgccgcaa gcgctgatga caaaaacgcg cccctcgaaa
      781 gcatgatatt ttgcccgaat gtcgatcgca tcgaagtttc gcttcccggc ggggaaagcc
      841 ggccaggaag cgatatggat gcgttcgttc tgagccaaaa gggaaaagcg cgccaggcta
      901 ttggtgttct cgccgcagat gagaccgcca atattcccat agggcgtttc ataggtccgc
      961 atgctcgaac cgtcgcccga cgcatggacg agacgctctg tcattgttgg catgagctta
     1021 cgatgaaccc caagcagatc accctctgga ccgaaaaaca gaagcgaatt gtaaagcgtt
     1081 cccatcgtac cgacatcgcg ttcgctggcg ccggccacaa cgtagacttt gcttctcttg
     1141 gccgccgcgc caatcgtatc gatcgctgcg ctcggtacct caatagcatt tttgaagaga
     1201 gatttactat gcgctatcga ttcggcactg cgaggcatga accaatgaaa ccaatcagga
     1261 aagccgggca aaaacccttc cggaaatccg ataatcttcg cgccgtggtc ggctgcctcc
     1321 tcaataagac ggcaagcctt agagacggtt gcatcacgat cgagccagac cggcgcggct
     1381 tgaacggctg caagacgcac agtcggataa ttatcaccca tatcaacgat ctccagagat
     1441 acggactcga ctcgctttag tagagaggca tttgctcatt tcgcggcgcg gttttcggac
     1501 cgcaccggcc cccttcgttt taaacttatt tgatgagccg gtgcgaaagc gcctcggtgc
     1561 ggatcgacgt atcaacaaag ttctctatat cgaccggcgc gctgagattg ccggtatcga
     1621 tcagcaattg cttcatccag ccgagcttct ccatcggcac attcattgtc gggtcgagga
     1681 gattcaactt gagagcctga tcatagatat aggccggacg tggatcattg agcttgatgt
     1741 tcagcgtttt gaccgtcagg tcgatagacg cctgcttgtg ctccatcgcg aagcggacac
     1801 cgtccatttg cgcggcaaca aagctcacca cctcggcgcg atcctgcgca attttactgg
     1861 cggaaacata ggtgcaccac cgcaggaagt tcggcaattc ctgatgcgcg tcaaacagga
     1921 gatgaacctg gtggtccgca gcaaagggca cgaattccgt cgagattgcc gcggcatcga
     1981 ccagcccctg gctcaaggca cgaaagcggt ccgcgtcgcc gccgagcgac gcaaaacgca
     2041 cgcccgaaag cgggatgttg tgcttgcgca aaatgccgcg cgaaatcaga tccggcaacg
     2101 agccgggatt agagatcgcg aacgttccgt tcttaagctg atcgatagtg gcgattttat
     2161 tggtcgagaa aatcccatag gacagacccg gccaatagca tccgacgagc ttgatattgg
     2221 caccgcgcga cgccgcaata atggccccgc cgggggttcc ttcgtagctg tcaacatcgc
     2281 cggcgataag cgccttcaaa gccagggcgt cgcctttcac cggaacgtat tcgatattga
     2341 gcccatgcct gccggcaatg tcggatttgc ctgccatcat ggcgatgcca gagtccgatt
     2401 tcggttcgac gatggcatgg cgccaggtcg ccgtctcggc atcggctgtc cccgctgcgc
     2461 caagtaagag aagcagcgcg taagaaatct ttctgatcat gtgtccgggt ttcctgaaat
     2521 cggcgcgttc atctcgacat cgcttcgccg ctgcttggca attctctcgc cagttttgaa
     2581 gtattcactt tgagttgcga gcttcgcctg ctcttccagc caatctggcc aattgccgag
     2641 atcgtagcca aaccaagggc tctccggctg caagggacga agcccgagct tgttccaaag
     2701 ctcgcgcgcc tcttccatga attcccgttt cggcaatgcg atcggtggaa agtcgccgcg
     2761 catcgtcgcg ttgatcaaaa cggatgcgct gcccgcggaa ttggtctcgt tgcgcgggcc
     2821 gtggcccggg tcttttttgt cgagaatctt gaggtcgttc tgcggctggc accggtagca
     2881 catcgcccag aaaatcgcat cggcattttc cggatcgata tcctcatcga ccgcgacaat
     2941 ccatttgcca gcatagcgat gcagcgaagc tgcgccatag agcgcgcgcc aaatctccgt
     3001 ctccaaagtg ttgcgagcaa attggatgat aaaaagcgcg ataacgctcg tcatcggctc
     3061 atgcatgtga acccgcttga cgcccgtgac gcccattgtc gaacgcagat gattgaaaag
     3121 aaccggctcc attgccgcgc gacggatgac gctggactcg ctgggcgtga cctgactgat
     3181 gaaggatggc aggatggggt tgcgccgccg ggtgatcgcc gtcacttcca tataggcgtt
     3241 gtattcctgc aaatggacat agccgtggga ttcgccaaac ggcgcttccg gttcccgcat
     3301 aaccgtttca atgaatccct cgatcacgat ctcggcttgc gctggcacga gaagatcgac
     3361 cgtcttcgcg cgcacgacct caatcggcgt gccggtgagg gcaccggcaa cccagacctc
     3421 atcgagactt tccggcatct tctgcacggc tgcgtatgag accgccggcg gacatccgag
     3481 aacgatggcg cagggaagcc gttcgccgcg cgccttatgc ttttcccaat gcacaaggat
     3541 gccgggccgg atctcgacgg acgggttcat gccgatgcgg cgtggcgctt taatctgccc
     3601 gcgataattg ccgacattct gaattcctgt atcgggatcg cgcgtgatgt aatgccctgc
     3661 ggtcaaataa ggtgcattgt cccacccggg tgtcgagatt ggcacgggaa gagaatcgag
     3721 gccgcggccc ggctgatcga gctcgtcatg gagcgtcacg atatcgtgac atgctgcatg
     3781 ttcgacgacg accggcgcga gcggcgtagc gagcgcctta agccaacggt cgcgaatggc
     3841 gtcgagatcg gcaccgaagc cgacacgata aatctcgcga ctgccggcga gacccgcgac
     3901 aagcacagcg ccgtcatatt tcctgccttt cgaatccgtc ggattcaaaa acagaaaagc
     3961 gcgccgctcc gcttcaggaa tgctgccgcg gtactgccac cgcaccagcg gatgcatgat
     4021 cgtatcttta ttgaccggct cttcgacaac aaagagaagg cccgcgtcgg caagatttcg
     4081 cacgtgatcg tgcaaatctg gatagaggtg agtcgcccca gtttcacgcg cgtagcaact
     4141 aagttttgat ttcagatcat ccgaaattgc cacaacgcac cacaatattt cacgcaggga
     4201 tgagcggagc ccgatgccat tatttttggg gccctcaaaa ccgtaagatt gtatgacgac
     4261 gttagtcaag gcaatttctg atcgcgatgt ttatacgccg cccgtgcctg cttagtttgc
     4321 agcgcctagt aacgctctca aaatatcgtc ttccaacctt acgatttggc acgaaagctg
     4381 cctcttgatg ccgtgtcggt atagtcggcg aaatgcaggg acttcatgaa catgatctgc
     4441 acgctgtttg ggcgtttatt aatcgccgcg ccaattattg cctacagttt gggcgcgatc
     4501 gctccgggta ctgccaagac ataattcgta aagacctccc ctcgactcag agaatgaatg
     4561 atggctgcac gtaagcgctt tgtatggcgt gtcgcagggc tggcattgtc ttgcattgcc
     4621 ggattttctg cttccgatct tgtggccgcg ccggaccaaa ccggcattgt tcatacggac
     4681 gcgggaaacg tccggggttt gatcaacggt gatgtgcgcc gctatctcgg catcccttac
     4741 gcggcgccgc ccattggtgc gctacgctgg cgaccgcccg tcaatgcgcg tccatgggtg
     4801 actgttctgg atgcgacgaa atttggcgga acttgcgcgc aggctcagcg cggcgttttc
     4861 gcggcaccaa gccaaaacga ggactgtctc tatctaaaca tttatacacc acgggaaaca
     4921 aacgcggcag cgcgcaatcc cgtgatggtg tggtttaacg gcggtggact ctttagcggt
     4981 gaaagcaacg attatgacgg gagcaaactc gttgcgcgcg gcaacgtgat tgtcgtcaca
     5041 ttgaactatc gcgttggcgt gttcggattt ttctcgcatc ctgccttaaa cgccgaaggg
     5101 catccttacg caaattacgg catcatggat cagcagtttg cgctgaagtg ggtgcagagg
     5161 aacatttcgg cttttggcgg cgacccgaac aacgtcacga tttttggtca atcgggtggc
     5221 ggcaccagcg tgatggcaaa tttggtctcg ccgctttcga aaggtttatt tcagaaggcc
     5281 atcaacgaaa gcggaaccag tatccaggtc gtaacgccag ccgcgtccct cgacgatggc
     5341 gtgcaattgg ccaattcagt cggctgtgcc ggcgcggact cggcgaagtg catgcgtgca
     5401 ctcagtgtta agcagatcct tgatcatcaa gcgccgattc tccgccacgt tgtcgtcttc
     5461 ccggcgattg acggcacggt cattacgaaa acggcgttgg acgctttcag ccacggctca
     5521 ttcaatcacg ttccgattat gacgggcctt gtcgccgacg agcaaagctt tttcatgtcg
     5581 gaactcaaga cgcataaacc cgcgctgcca gcggagtatg aggcgctcgt cgcctcgttt
     5641 ggacatcaaa atcgagataa aatcgcagcc cgctatccgt tgaacaaata tccgagcccg
     5701 gccttggcgg aagtggcggc cgcgcaaggc ttcaaatcat gcaccgcgcg ccttcttgac
     5761 cgcgcttggg cgaaatacgc gccggtctat gcctatgaat tccgcgacca aaccgcgccg
     5821 tcctattttc cgaaattcag ctttccgatg cgcgcatatc atacggccga actgcaatat
     5881 atttttcccc gatttcacgg cggccggggt acgccgcatg cgctgaacgc ggcgcaggag
     5941 aagctttcgg atcagatggt ggattattgg acgagtttcg cgcggacggg caggcctgct
     6001 atcagcgcaa gcccgccgtg gaagccttac gatgcgcaaa ccgataatta tcaggtgctg
     6061 gatctatcgg gcgtgagcaa cgtcggtggc tatgggaaga catacgattg cgaactttgg
     6121 gacgcgattc tttcattatg atccgatcca tcagagttcg cgacataact gaattgcggg
     6181 atcataacct agacgcctat tccggcgg
//
LOCUS       DASWOG010000341         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_282680,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000341 DASWOG010000000
VERSION     DASWOG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282680"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VGB93_07160"
     CDS             <1..809
                     /locus_tag="VGB93_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390554.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0145915.1"
                     /translation="DQRLIPYSFTSTYGRAFFVGLLNTLLVAAVGIVCASLLGFAIGA
                     ARLSPNFVVAKCAAIYVEALRNVPLLLQILFWYNAVLKALPVPRASLVLPGHIFLNNR
                     GLFVPWPTFSPFGISTPVFHGFNFSGGTKLSPEFVALTLSLILYTAAFIAEIVRAGIA
                     GVPKGQGEAADALGLNAAQTRKFVVVPQALRLIIPPLTSQYLNLFKNSSLAVFIGYPD
                     LVQIFTGTVLNQTGAAVPVIAVTMAVYLFVSLVGSLAMNLYNRRILRVVR"
     gene            809..1939
                     /locus_tag="VGB93_07165"
     CDS             809..1939
                     /locus_tag="VGB93_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0145916.1"
                     /translation="MVATFVRSEPAPVSPPPKAAALFAPFVRNLFGSSASIVLTLLAV
                     ALVVWLVPDLLRYFIFNAVWHGDAAACRNADGACWVFIREKLPYFIYASYPLDQRWRV
                     NLVFAIGAILAVWLLSPSAPERRLGMWLGFAVYPIVAFVFLYGAPMLGLRQVSSDLWG
                     GMLVTLVVATFGIVVSLPLGILLALGRRSRLPAVHFASAAFIEFMRGVPMIAVLFMAD
                     TMLPLFLPEGVEPNRLVRPLVGVALFASAYMAEVIRGGLQALPKEQSEAGEALGLRFW
                     SIQRLVVLPQVLRLTLPAITNTFIGLFKDTTLVATVGIFDFLRTVDSARLDPTWSSPT
                     ITTTAYTFAALVYFVCCFGMSRYAQSLERRFGNATAARRLED"
     gene            1941..>2255
                     /locus_tag="VGB93_07170"
     CDS             1941..>2255
                     /locus_tag="VGB93_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145917.1"
                     /translation="MTDTSPLAVEIAHLNKWYGQFHVLRDIDLSVAEGERIVICGPSG
                     SGKSTLIRCVNGLEEFQQGSLAVDGMALNRNSLAAVRRDVGMVFQHFNLFSHLTVLEN
                     CTL"
BASE COUNT          327 a          690 c          672 g          566 t
ORIGIN      
        1 tcgatcagcg tctgattccc tattccttca cctcgacata cggccgcgca tttttcgttg
       61 gcctgctcaa tacgctgctg gtcgcggctg tcggcattgt ttgcgcgagc ctgctgggct
      121 ttgcgatcgg tgcggcgcga ctgtcgccca attttgttgt cgcgaaatgc gccgccatct
      181 atgtcgaggc tttgcgcaac gtaccgctgc tgttgcaaat tctcttctgg tataatgcgg
      241 tcttgaaggc gctgccggtg ccgcgcgcct cgctcgtgtt gccgggacat atctttctca
      301 ataatcgcgg cctgtttgtc ccctggccga ccttttcgcc gtttggcatt tcgacgccgg
      361 tctttcacgg attcaatttc agtggcggga cgaaactgtc gccggaattc gtcgcgctga
      421 cgttgtcgct gatcctctat accgccgcct tcatcgccga gatcgtccgg gccggtattg
      481 caggcgtacc gaaggggcag ggcgaggcgg ccgacgcact tggcctcaac gcggcgcaga
      541 cgcgcaaatt cgtcgtcgtg ccacaagctc tgcgactcat cataccgccg ttgacgagcc
      601 aatatctgaa ccttttcaag aattcgtcgc tggcggtctt catcggctat ccagatctcg
      661 tgcagatttt cacgggaacc gtgctcaatc agacgggtgc cgcggtgccg gtcattgcgg
      721 tgacgatggc ggtctatctt ttcgtttcgc tcgtcggctc gctggcaatg aacctctata
      781 accgccgcat cctcagagtg gtgcgctgat ggtggcgacc ttcgttcgca gcgaaccggc
      841 gcccgtgtcg ccgccgccga aagccgcggc attattcgcg ccgttcgtgc gcaatctttt
      901 cggctcgtcg gcctccattg tgctgacgct tctggcggtc gctctggttg tctggctcgt
      961 tccggacctt ctgcgctatt ttattttcaa tgcggtctgg cacggcgacg ccgcggcgtg
     1021 ccgcaatgcc gacggcgcct gctgggtctt catccgcgag aagctgccgt atttcatcta
     1081 tgcttcgtat ccgctcgacc agcgctggcg ggtcaatctc gttttcgcaa ttggcgccat
     1141 tctcgctgtc tggttgctga gcccgagtgc acccgagcgg cgcctcggca tgtggctggg
     1201 gttcgcggtc tatccgatcg tcgctttcgt gtttctctat ggcgccccga tgctcggact
     1261 ccggcaggtg agcagtgacc tttggggcgg gatgctggtg acgctcgtcg ttgcgacttt
     1321 cggcattgtc gtttcgctgc cgctcggcat cctgcttgcg ctgggacgcc gctcgcggct
     1381 gccggcggtg cattttgcca gcgctgcttt catcgagttc atgcgtggcg tgccgatgat
     1441 cgccgttcta ttcatggccg atacgatgct gccgctgttt ttaccggaag gtgttgagcc
     1501 gaacaggctc gtgcggccgc tcgtcggtgt ggcgctcttc gcctccgcct atatggccga
     1561 ggtaatccgt ggcggcctac aggcgctgcc gaaggagcaa agcgaggcgg gcgaggcgct
     1621 cggcctgcgc ttttggtcga tccagaggct tgtcgtgttg ccgcaggtcc tgcgactgac
     1681 gctgccggcg atcaccaata cgttcatcgg cctcttcaag gatacgacac tcgtcgccac
     1741 cgtcggcatt ttcgattttc tgcgcacggt ggattcggcg cggctcgacc ccacctggtc
     1801 aagcccgacg atcaccacca ccgcctatac attcgccgcg ctcgtctatt tcgtctgctg
     1861 tttcggcatg tcgcgttatg cgcaaagtct cgagcggcgc tttggcaatg cgacggccgc
     1921 gcgacggctt gaggattaga atgaccgaca cttccccgct ggccgtcgag atcgcgcatc
     1981 tcaacaaatg gtacggccaa tttcacgtct tgcgtgatat cgatctcagc gtggccgagg
     2041 gcgaacgcat cgtcatttgt gggccgtcgg ggtccggcaa atcaacgctc atccgttgcg
     2101 tcaacggtct ggaggaattt cagcagggtt cacttgccgt cgatggtatg gcgcttaatc
     2161 gcaattcgct ggcagcggta cggcgcgacg tcggcatggt gtttcagcac ttcaatctgt
     2221 tctcgcatct gaccgttctg gaaaactgca cgctc
//
LOCUS       DASWOG010000342         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319874,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000342 DASWOG010000000
VERSION     DASWOG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319874"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB93_07175"
     CDS             complement(<1..236)
                     /locus_tag="VGB93_07175"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550290.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0145918.1"
                     /translation="MSNKMWGGRFADSPDAIMEEINASIDFDQKLAAQDIAGSLAHVA
                     MLGETGILSKADAAAIAEALGRIRGEIENGQFAYS"
     gene            272..940
                     /locus_tag="VGB93_07180"
     CDS             272..940
                     /locus_tag="VGB93_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0145919.1"
                     /translation="MSDQETKKRGLPSPVTSIIALGLVVMFIYGMKGWRRNEAASAAS
                     CPAAHAIAARLAPLVHGEVAALAIDSNPQPMPALTFIGPDGKPTTLAAFKGRNVLLNL
                     WATWCVPCRMEMPSLDKLQGARGSDDFSVVAVNIDTAKLDRPKAFLKEIGVKNLSFYA
                     DNSANIFSTLKQEGKVLGLPTTILVAKDGCDIGTMAGPAKWDSPDALSLISAQKQAAE
                     TPKS"
     gene            945..1307
                     /locus_tag="VGB93_07185"
     CDS             945..1307
                     /locus_tag="VGB93_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145920.1"
                     /translation="MCSEQLKMEDLKMRLLISMLLGVSLSAAIMAPAFSEPLALASCA
                     KFTSARDEKASLFAGYIYGFVAARMGYKDEKRLTAAAVKVRADALKICQKTSDASFAK
                     IIDALTADLAKRSWLQKL"
     gene            1345..2253
                     /locus_tag="VGB93_07190"
     CDS             1345..2253
                     /locus_tag="VGB93_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0145921.1"
                     /translation="MLEGLPPNNAAFLMRLRCADSTTAQRVADIIVETFDPAETAASA
                     FEETGDRPDVEGPWLVEAYFGTVPDEAKVRDLVSLAAGEELAAEAEFGRVAKTDWVAA
                     SLAGIAPVRAGRFLLHGEHDRAIAQPNDLAIEIEAALAFGTGHHGTTRGCLIFLDAIA
                     KRRRARKILDVGTGTGVLAIAAAKAFRRPVAAGDIDPVAVATAKVNSTRNGAGSFIHI
                     VQARGLDHPALRGDYDLITANILAAPLRRLAPALAKALLPEGEIILSGLLGRDVPGII
                     STYRTQHLRLVARLDIDGWATLLMRR"
     gene            2357..3589
                     /locus_tag="VGB93_07195"
     CDS             2357..3589
                     /locus_tag="VGB93_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0145922.1"
                     /translation="MSSDTIVIVGAGHGGVQLAASLREEGYGGKLVLLGDEKDLPYQR
                     PQLSKAYLKHATPEDGVLLRGPDFYAQNNIELLLGERVTEIERDARRLHLKSGKTLEY
                     TKLVLATGGIQRRLPIEGADMEGVFALRTMEEARALRDKLATSQNVIVIGAGFIGLEF
                     AATAASQGRKVDLFELADRPMGRVVTPQTSAFFAQAHKAFGVNLHFGQSILAIKGDGT
                     SVTCVVLDDGATMPADMVLVGIGLVPSDELARAAGLDAPNGIKVNEKLATSDENIFAI
                     GDVVYHYNTHRGAEMRLESVQNATDQARTLAKLLVGKPANYDHVPWFWSDQGDLKLQI
                     AGFLDESDTIVMRGSFDERAYSLFGFKNGVLTGVESVNKGGDHMLARRLLGEHIAVTP
                     EFIADPASDMKSLIRPKR"
     gene            3841..>4623
                     /locus_tag="VGB93_07200"
     CDS             3841..>4623
                     /locus_tag="VGB93_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145923.1"
                     /translation="MPLAAGVWAFPGLGTTGKAWAGSFDITADTTAAETLSDNQTGTV
                     AAGVTLTNATKNTVVVTVTGTGTSLTNDGTISQTNTGGGRAVRVTGTGVSFTLTNAGG
                     ATISAAGDDAIQSQHDASVTIENSGTIESLGGGQAIDFNAITTGTNEVYNYSAGLIEA
                     FGADALRPGVNGYIYNAGTIRSENVPGNDTGNDGIDAQSNTGVTIDNVGLVDGARHGV
                     TGGNTDTTTDGSFAMTVTNEAGGTIVGEDGSGINIDGFNGNEV"
BASE COUNT          920 a         1430 c         1407 g          866 t
ORIGIN      
        1 gaataggcga attggccgtt ctcgatttcg ccacggatgc ggcctagagc ctcggcgatg
       61 gcggcggcat cggctttcga cagaatgccg gtttcgccca gcatcgcgac atgggcgagc
      121 gagcctgcta tgtcctgcgc cgcgagcttc tggtcgaaat cgatcgaggc attgatttct
      181 tccatgatcg cgtcggggct gtcggcgaac cggccgcccc acatcttgtt gctcattggt
      241 tccgctttca aagatcggcc gaagttcatt tatgtcagat caagagacaa agaagcgcgg
      301 cctgccgtcg cccgtcacat cgatcatcgc cctcgggctt gtggtgatgt tcatatacgg
      361 gatgaagggc tggcgccgca acgaagccgc gagcgccgcg tcctgccccg cggcgcatgc
      421 gatcgccgcc cggctcgcgc ctttggtcca cggcgaggtc gcggcgctgg caatcgacag
      481 caatccgcag ccgatgccgg cgctcacctt catcggcccc gacggcaaac caacgacact
      541 cgcggccttc aagggccgta acgtgcttct gaacctttgg gcgacctggt gcgtgccctg
      601 ccggatggaa atgccctcgc tcgacaaatt gcagggcgca cgcggcagcg atgatttctc
      661 tgtcgtcgcg gtcaatatcg acaccgccaa gctcgaccgg ccgaaagcct tcctgaagga
      721 aatcggcgtc aaaaatctga gtttctacgc ggataatagt gccaacatct tctcgaccct
      781 aaagcaggaa ggcaaagttc tcggcctgcc gacgacgatc ctcgtcgcca aggacggctg
      841 cgacatcggc acaatggcgg gtccggcgaa atgggattcg cccgacgcct tgagcctgat
      901 cagcgcgcag aagcaggcgg ccgagacccc gaaaagctga tggcatgtgt tccgaacaat
      961 tgaagatgga agatttgaaa atgcggctgc tgatttcgat gcttttaggc gtctcgctgt
     1021 cggccgccat catggcgccg gcattctctg agcccctcgc gctggcatcc tgcgcgaagt
     1081 tcacctccgc ccgtgacgag aaagcatcgc ttttcgccgg atacatctac ggattcgtcg
     1141 cggcgcgaat gggctataag gacgagaagc ggctcaccgc cgccgccgtc aaggttcgcg
     1201 ccgacgcgct gaaaatctgc cagaagacct ccgatgcatc cttcgcgaaa atcattgatg
     1261 cgctgacggc cgatctcgcc aagagatcct ggctgcagaa gctttgaggc ttgggccgcg
     1321 cgccggcctg tgctacccgc cgccatgctc gaaggcctgc cacccaacaa cgccgctttt
     1381 ctgatgcgcc tgcgctgcgc cgattcaacg acagcgcagc gcgtcgccga tattatcgtc
     1441 gaaaccttcg acccggcaga aacggcagcc agcgccttcg aggaaacagg cgaccggcct
     1501 gacgtcgaag gaccgtggct cgtcgaagcc tatttcggca cggtgcccga cgaagccaag
     1561 gtgcgtgacc tcgtcagcct cgcggccggt gaggaactcg ccgcagaagc ggagttcggc
     1621 cgtgtcgcga agacagattg ggtcgcggcc tcgctggccg ggatcgcgcc ggtgcgcgcc
     1681 gggcgctttt tgctccacgg cgagcacgac cgcgccatcg cgcaaccgaa cgatctcgcc
     1741 atcgaaattg aagcggcgct tgccttcggc accggccatc acggcacgac gcgcggctgc
     1801 ctgatctttc tcgatgccat cgccaagcgc aggcgggcgc gcaaaatcct cgatgtcggc
     1861 accggcaccg gcgtgctcgc catcgccgcc gccaaggcct ttcgccggcc ggtcgcggcg
     1921 ggcgatatcg atcccgttgc ggttgccacc gcgaaggtca actcgacgcg taacggcgct
     1981 ggcagcttta tccatatcgt acaggcgcgc ggcctcgatc atccggcgct gcgcggcgat
     2041 tacgatctca tcaccgccaa catattggca gcgcccctgc gccgtctggc gccggcgctc
     2101 gccaaagctc ttttgcctga aggggaaatt attctctcag gccttctcgg ccgcgatgtt
     2161 ccgggcatta tcagcaccta tcggacgcag catttgcgcc tcgtcgcgcg gcttgacatc
     2221 gacggctggg cgacgctttt gatgcgccga tagagcctcg gcctgaaccg aggctctcat
     2281 ttttagtttg acgcgttttc ttcacgcgaa ccggtatcca cttcgctcga aaacgctttg
     2341 actaagggaa aaacggatga gttcggatac gattgtcatc gtcggcgcgg gccacggcgg
     2401 cgtgcagctt gccgcttcgc tgcgcgagga aggatatggc ggaaagcttg tccttctcgg
     2461 cgatgaaaag gatcttccgt accagcggcc gcagctttcc aaagcctatc tgaagcatgc
     2521 gacgccagag gacggcgtgc tgctgcgcgg cccggatttc tacgcccaga ataacatcga
     2581 gcttctgctt ggcgagcgcg tcaccgagat cgagcgcgac gcgcgccgtc tgcatctgaa
     2641 atccggcaag acgctcgaat acacaaagct tgtactagcg acgggtggca tacagcggcg
     2701 tttgccgatc gaaggcgcag acatggaggg tgttttcgcg ctgcggacaa tggaagaagc
     2761 acgcgcgctt cgcgacaagc tcgcaacgtc gcaaaacgtc atcgtcatcg gcgccggttt
     2821 catcggcctc gaattcgctg cgacagcagc atcgcaaggc cgcaaggtcg atctctttga
     2881 attggccgac cggccgatgg gccgcgtggt gacgccgcag acatcggcgt tctttgcgca
     2941 ggcgcataag gcttttgggg tcaatctgca tttcggccag agcatcctcg cgatcaaggg
     3001 cgacggcacg agcgtcacct gcgtcgtctt ggacgacggc gcgaccatgc ctgccgacat
     3061 ggtgctggtc ggcatcggcc tggtgccgag cgacgaactc gcgcgcgccg cggggctcga
     3121 cgcgccgaat ggcatcaagg tcaatgagaa gctcgccaca tcggacgaaa acatctttgc
     3181 aatcggcgat gtcgtctatc actacaacac gcatcgcggc gccgagatgc ggcttgaatc
     3241 tgtgcagaac gcgacggatc aggcgcgcac gctggcaaaa cttctcgtcg gcaagccggc
     3301 gaattacgat catgtgccgt ggttctggag cgaccagggc gatctcaaat tgcagatcgc
     3361 cggcttcctc gacgaaagcg acacgatcgt catgcggggc tctttcgacg agcgcgccta
     3421 ttcactcttc ggcttcaaga acggcgtgct gaccggcgtc gaatccgtca acaagggcgg
     3481 cgaccatatg ctggcgcggc ggctgcttgg tgagcacatc gccgtgacgc ctgagttcat
     3541 cgccgatccg gcttcggata tgaagtcgct gataaggccg aagcgatgag ggccagtacc
     3601 gcctcgccct tgatgtccgc cgaacggaga tgagcgtcgt gatgaatcag ccacactgat
     3661 tcttaatgct caatttcgcg aaaaggctct gcgaaacgtc atccgactgt catgcgctaa
     3721 aaataccgcc gatgcggaat gaaataacac atcggggtgg acataatgtt gcgtttggtt
     3781 ggtgccggcc gcgggttaag cggcaagtcg ttttctagct tcgttcgagt ttcctgttca
     3841 ttgcctttag ctgcgggtgt ctgggcgttc ccggggctcg gcactaccgg aaaagcctgg
     3901 gccggatcgt ttgacattac cgcggacacg accgccgcgg aaactttgtc ggacaatcag
     3961 acaggaacgg tcgcagcggg cgtcactttg accaacgcca ccaagaacac ggttgttgta
     4021 acggtcaccg gaaccggaac ttcgctgacc aatgacggaa cgatttcgca gacaaacaca
     4081 gggggcggcc gcgcagtacg cgtcaccggg acaggcgtta gtttcacctt gaccaatgcg
     4141 ggcggggcga ccatctccgc cgccggcgac gatgcgatac aatctcagca cgacgccagc
     4201 gtcacgatcg aaaattcagg caccatcgaa tctcttggcg gcggccaggc gatcgacttc
     4261 aatgccatca cgaccgggac gaacgaggtt tacaactatt cggcggggct gatcgaggca
     4321 ttcggcgccg acgcgctgcg ccccggcgtc aatggttaca tctataatgc cggcaccatc
     4381 cggtcagaga acgttcctgg caacgacacc ggcaacgatg gcatcgacgc gcagagcaat
     4441 acgggcgtga ccatcgacaa cgttggcctc gtcgacggcg cgcggcacgg tgtcacgggc
     4501 ggcaacaccg atacgaccac ggacggctcg tttgcgatga ccgtcaccaa cgaggctggt
     4561 ggcacaatcg tcggcgagga cggatcgggc atcaacatcg atggcttcaa tggcaacgag
     4621 gtc
//
LOCUS       DASWOG010000343         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_264924,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000343 DASWOG010000000
VERSION     DASWOG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264924"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="gluQRS"
                     /locus_tag="VGB93_07205"
     CDS             complement(<1..881)
                     /gene="gluQRS"
                     /locus_tag="VGB93_07205"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY0145924.1"
                     /translation="MTRSYISRVFRFAPSPNGYLHLGHGFSALLNQEMALAAGGKLLL
                     RIEDIDLERCRPEFEQAIYDDLAWLGLDWQQPVRRQSEHFDAYSEALTGLKKRGLVYP
                     CFCSRADVVAAASEQADVKRDPDDSPLYPGTCRHLSPAERRRRLDAGQSATWRLDMAA
                     ARKEVRHAIDWREYDGGNEPRFVEAEPALWGDVVLARKDVPASYHIAVVVDDALQGVT
                     DVVRGEDLFMATGLHRLLHILLDLPSPYYHHHELLRDAAGRKLSKSLRARSLRALRQD
                     GFTPAEIKAQLGLNERS"
     gene            1072..1770
                     /locus_tag="VGB93_07210"
     CDS             1072..1770
                     /locus_tag="VGB93_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0145925.1"
                     /translation="MAPSLDGPRLAPKGHAKQLVVFLHGYGADGNDLIAIGEQWRDFL
                     PGTAFAAPHAPEPCAQAPVGRQWFPLSLRDESELWPGVNGAQQGLDQFLDAELARYGL
                     SDDRLALVGFSQGTMLALHVGLRRKRAPAAILGYSGLLAGPEHLANAQARDAKGGRPP
                     ILLAHGDQDDVIPVEAMFVAAEQLAEADIPVQWHLAPGLGHGIDGSGLLHGGLFLAKS
                     LGLKLELPTATAPR"
BASE COUNT          354 a          603 c          606 g          349 t
ORIGIN      
        1 aagctgcgct cattcaagcc gagttgggct ttgatctcgg ccggcgtgaa gccgtcctgc
       61 cgcagcgcgc gcaacgatct ggcccgcagg cttttcgata atttgcgccc tgcggcgtcg
      121 cgcagaagct cgtgatgatg ataatacggc gacggcaggt cgagcagaat gtgcagcaaa
      181 cgatgtaagc cggtcgccat gaagagatct tcgccacgca cgacatcggt cacgccttgc
      241 agcgcatcgt cgacgacaac cgcgatgtga tagctcgcgg gaacgtcctt gcgcgccagc
      301 accacgtcac cccagagcgc gggctctgct tcgacaaagc gcggctcatt gccgccatcg
      361 tattcgcgcc aatcgatcgc gtgccgaacc tctttccggg cggccgccat gtcgagacgc
      421 cacgttgcgg attgtccggc gtcgagccgg cgcctgcgtt cggccggcga aagatgacgg
      481 caggtgccgg gataaagcgg cgaatcgtcc ggatcccgtt tcacatcggc ttgttccgat
      541 gcggctgcca ccacatcggc acgcgagcag aaacagggat agacaagccc gcgtttcttc
      601 aggcccgtca aagcttcgct ataggcatcg aaatgctcgg attgacggcg caccggctgc
      661 tgccagtcga ggccgagcca ggcgagatcg tcatagattg cctgctcaaa ttccgggcgg
      721 cagcgttcga gatcgatgtc ttcgatccgg agcaggagct tgccgccggc ggcaagcgcc
      781 atttcctgat tgagcaatgc cgaaaagccg tggccgagat gcaaatagcc attcggcgaa
      841 ggcgcgaagc gaaagacgcg cgaaatatag gatcgtgtca tcactgggac tcgtcgctac
      901 gtccgctggc cggtctgcgc cccggcgcgc atcgattata gaggcaatgg accccaaatg
      961 ggacgtccct gcggcgcagc aagactcgcc agcgcagcaa gacttgccgg gcatgtcact
     1021 ttcttgcaag aacgatctta gcttgccttg gttctctcct ggaattttta gatggctccc
     1081 agtctcgacg gtcctcgcct cgcgccaaag ggccacgcca aacaacttgt cgttttcctc
     1141 catggctacg gcgccgacgg caatgatctc atcgccattg gcgaacaatg gcgggacttt
     1201 ttgccgggca cagcttttgc cgcgccccat gcgccggaac cctgcgcgca ggcgccggtc
     1261 gggcggcaat ggtttccatt gagcctgcgc gacgaaagcg aactttggcc gggcgtcaac
     1321 ggcgcacagc aggggctcga tcaatttctc gatgcggaac tcgcgcgcta tggcctgagc
     1381 gatgaccggc ttgccctcgt cggctttagc caggggacca tgctggcgct gcatgtgggc
     1441 ctgcggcgca aacgcgcgcc cgcggccatt ctcggctatt ccggccttct tgccgggccg
     1501 gagcatctgg cgaatgcgca ggccagggat gccaagggtg ggcgcccgcc gatccttctt
     1561 gcgcatggcg atcaggatga tgtgatcccg gtcgaggcca tgttcgtcgc cgcggagcaa
     1621 ttggctgagg cggacatccc ggtccaatgg catctggcgc cgggcctcgg ccacggcatc
     1681 gacggcagcg gcctgctgca tggcggcctg tttctggcga aatcgctcgg gcttaaactg
     1741 gagcttccga cggccacagc accgcgctag gcgctcaccc cggcgcaatg ccagcgggcc
     1801 ttgtgccaag taccggctgc aaaggctgta gagcgtgaga cgtgcgccgg acgccgaccg
     1861 gacggtagag ctgcttcatc gcgtccacga aaatgatgcc ggcaccgggc ag
//
LOCUS       DASWOG010000344         4582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_319910,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000344 DASWOG010000000
VERSION     DASWOG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319910"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VGB93_07215"
     CDS             complement(<1..577)
                     /locus_tag="VGB93_07215"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0145926.1"
                     /translation="MFYAPEHRDRHLLPHDPFKALIAPRPIGWVSTKSKSGALNLAPY
                     SFFNAVADHPPIVMLSSQGWKDSATFANETREFVWNMATYDLREPMNLSSANLPRGES
                     EFAYAGLETAPSRFVAPPRVAQSPAALECKVIDILLLKDLDGALTGNVLVFGQVVGVY
                     VDDAFIKDGRVDTSAMRPIARCGYMDYAVTDK"
     gene            complement(588..1178)
                     /locus_tag="VGB93_07220"
     CDS             complement(588..1178)
                     /locus_tag="VGB93_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0145927.1"
                     /translation="MKKVINLNNSMIELLQKRRSAPPATMAGCAPSVEERDLLLTLAS
                     RVPDHGKIVPFRFIVFEGEGQQKAGEIVAKVYAKAHPDASTSELEKERRRLTMAPLVI
                     GVVSRTEPEHKVPEWEQVLCAGAVCFNLEVAANALGFASIWLTEWHSYDRAVLKRFGV
                     GKDEKIAGYIHIGHDSGPREDRARPKLADIVTKFEA"
     gene            1316..2266
                     /locus_tag="VGB93_07225"
     CDS             1316..2266
                     /locus_tag="VGB93_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145928.1"
                     /translation="MPALAVLFLVFALSAALPAAAQDTDSDAIAPMIPRGGLLGPRHD
                     NEAQTPHNTLLLSALLSGTDLHPVTSGLKWRVFDEATEADGSHRLVAESSEASPSFSL
                     PDGNYIVHCAMGLAGMTQRVMMAGAPTSERLLLNAGGLRIVPVLGDQPINPAKLTISI
                     YVPEHNNAEAKLILSKARAGDVIGLPEGNYHIVSTLLDTTGFGTMAQNGIANATNSVV
                     NADLRVQPGKLTDATLRHRAAVMTLKLVNAPGGEALANSAFTILTPGGDVIRELIGAF
                     PSLVLAEGEYVAIARHGDKTYQATFKVESTHDRDVEVVAR"
     gene            2367..2537
                     /locus_tag="VGB93_07230"
     CDS             2367..2537
                     /locus_tag="VGB93_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEY0145929.1"
                     /translation="MKYLLLSLPSLATLLVPFYNSTTPRLFGFPFFYWFLIVLIPASS
                     LVTYLVYKGEKR"
     gene            2534..4084
                     /locus_tag="VGB93_07235"
     CDS             2534..4084
                     /locus_tag="VGB93_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY0145930.1"
                     /translation="MIDHLNWMAAGVFIGFFLLVTVLGFVAAHWKRGDLNDLNEWGLG
                     GRRFGAWISWFLIGGDIYTAYTVIAVPSVVYAVGAYGLFAVPYTIIIYPLLYATFPRL
                     WAVAHRKNYMTAADYVLGRYGNNWLELAVAFTGIFATMPYIALQLVGMERVIDALGFQ
                     GHGIMSHLPLTIAFVVLALYTYRSGLRAPAMIAFVKDIMIYVFVLAAIVIVPMKLGGY
                     GAIFDAAGAALAKKAALSGGKIAAGLLLSPKQMLPFITLAIGSGLALLMYPHSMTGML
                     SARSGNTIRINAVALPAYSVMLALIALMGYMALAAGVHVTNPQDAVPHLILAVFPDWF
                     VGFCFAAIAIGALVPAAIMSIGAANTFTRNIWKPFIHPAMNSQEEAVLAKLMSLIVKV
                     GALAFILFVPTKFALDLQLLGGMWMAQVFPTVIFGLYTRWFNGWALLAGWATGMVLGT
                     WLAWTPAAWVPVHAIFGSNLNVYNGVTAFVANVLVAAVLSALLPNTARDETRAEDYAD
                     LPETAVAK"
     gene            complement(4090..4263)
                     /locus_tag="VGB93_07240"
     CDS             complement(4090..4263)
                     /locus_tag="VGB93_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145931.1"
                     /translation="MLKKIALALLTLGLAGCGPLGLPSPSASTSASAQQPQHLNDFSA
                     MRCGDATGPTTGC"
BASE COUNT          836 a         1407 c         1329 g         1010 t
ORIGIN      
        1 atttgtcggt cacggcgtag tccatatagc cgcagcgtgc gatcggccgc atggccgatg
       61 tatcgacccg tccgtctttg atgaaagcat cgtcgacata gacaccgaca acctgaccga
      121 aaacgagaac attgcccgtc aacgccccgt ccaaatcctt taagagtaga atatcaatga
      181 ccttacattc gagtgcagcc ggactttgcg cgacgcgcgg cggcgcgacg aaacgcgacg
      241 gcgcggtttc cagccctgca taggcgaatt cgctctcacc acgcggcaga tttgccgatg
      301 agagattcat aggttcgcgc agatcgtaag tcgccatgtt ccagacgaat tcccgcgtct
      361 cgttggcgaa ggtcgcggaa tccttccaac cctgggacga cagcatcacg atcggcggat
      421 gatccgcaac cgcgttgaag aatgaatacg gtgcgagatt gagcgcaccg cttttgcttt
      481 tcgtggaaac ccaaccgatt ggccgcggcg cgatcagcgc cttgaagggg tcatgcggca
      541 gcagatgtct gtcgcggtgc tccggcgcat agaacacgat tggcccctca tgcctcgaac
      601 ttggtgacga tatccgctag cttgggacgc gcccggtctt cgcgcggtcc cgaatcgtga
      661 ccgatgtgaa tatatccggc gattttctcg tccttgccga cgccgaaacg cttcaatacg
      721 gcgcggtcgt aactatgcca ttccgtcagc cagatactgg cgaagccgag ggcgttggcg
      781 gcgacttcga gattgaagca aaccgcaccc gcacacagca cttgctccca ttcggggact
      841 ttgtgctctg gttcagtgcg cgacaccacg ccgatcacca gcggcgccat tgtcagacgg
      901 cggcgttcct tttcgagttc ggatgtgctc gcgtccggat gtgccttcgc ataaaccttg
      961 gcaacgattt cgccagcctt ttgctggcct tcaccctcga acacaatgaa acgaaacggc
     1021 acgattttgc cgtggtcggg gacgcgggac gcaagcgtca ggagcaggtc gcgctcctcg
     1081 acgctggggg cacaccccgc catcgtcgca ggcggcgccg agcggcgctt ctgcaaaagc
     1141 tcaatcatgg aattgttcaa atttatcacc tttttcatcc aaattcggcg ctgctcatgg
     1201 catggaacgc cttgcggcgc acgtcatttt cgcgcgacgg cggctattgc tgcggccatg
     1261 acaaagccgg atttcctgtt agattctctc gatgatcctg ttcggtcgcc actccatgcc
     1321 cgctctcgcg gtgcttttcc tcgttttcgc gctgagcgct gcattaccgg ccgctgcgca
     1381 ggacaccgat tccgatgcca tcgcgccgat gattccgcgc ggcggccttc tgggcccgcg
     1441 ccacgacaac gaagcacaga cgccgcataa tactttgctg ctctcggcct tgctcagcgg
     1501 gacggacctg catccggtca cgtcgggtct caaatggcgc gtcttcgacg aagcgaccga
     1561 agccgatggg tcgcacaggc ttgtcgcgga atcgagcgag gcgtcaccgt cattctcgct
     1621 gccggacggt aattacatcg tccattgtgc gatgggcctc gcgggcatga cgcaacgcgt
     1681 catgatggct ggcgcgccga caagcgaacg gctgttgctc aatgccggcg gcctgcggat
     1741 cgtgccggtg ctcggcgatc aaccgatcaa tcccgcaaaa ctgacgattt cgatctacgt
     1801 gcccgagcac aacaatgcgg aggccaaact cattctctcc aaagcccggg ccggtgatgt
     1861 catcggcctg ccggaaggga attatcacat cgtctcgacg ctgctcgaca caaccggctt
     1921 tggcacgatg gcgcaaaacg gcatcgccaa tgcgacgaac tctgtggtca acgcggactt
     1981 gcgcgttcag cccggcaagc tgaccgacgc gacgctgcgc caccgcgccg cagtgatgac
     2041 gctgaagctc gtcaatgcgc cgggcggcga ggccttggcc aattcggcct tcaccattct
     2101 cacgccgggc ggcgatgtga tccgcgaact gattggcgcc tttccctcgc ttgtgctggc
     2161 ggaaggcgaa tatgtcgcca tcgcccggca cggcgacaag acctatcaag caaccttcaa
     2221 agtcgaatcg acgcatgatc gcgacgtcga ggtcgtcgcg cgctaatctc agcgaccgtg
     2281 cggcttgatc gcaattgccc gcttctgtaa gtcttgccgt ccggcgacga acttgccgcc
     2341 acgcgcaacg ccgcacggga ggggagatga aatatctact tttgagtctg ccctctttgg
     2401 cgacactgct ggtgccgttt tacaattcca ctacgccgcg tctgttcgga tttccgttct
     2461 tctactggtt tctgatcgtc ctgattcccg cctccagcct tgtgacgtat ctcgtctata
     2521 agggggagaa gcggtgattg atcatctgaa ttggatggcc gccggcgtct tcatcggctt
     2581 cttcctcctt gttaccgttc tgggtttcgt tgccgctcat tggaaacgcg gcgacttgaa
     2641 cgatctgaac gaatggggcc ttggcggacg ccggttcggc gcctggatct cgtggttcct
     2701 gatcggtggc gacatctaca ccgcctatac ggtgatcgcg gtgccctcgg tggtgtacgc
     2761 cgtcggcgct tacggccttt tcgccgtgcc ttatacgatc atcatctatc ccctgctcta
     2821 cgcgaccttc ccccggcttt gggcggtggc gcatcgcaag aattatatga ccgctgccga
     2881 ttatgtgctt ggccgctacg gcaataattg gctggagctt gccgtcgcct tcaccggcat
     2941 attcgcaacc atgccctata tcgcgttgca gctcgtcggc atggagcgtg tcatcgacgc
     3001 gctcggcttt cagggccatg gcatcatgag ccatctgccg ctcaccatcg ccttcgtcgt
     3061 cctggcgctt tacacctatc gcagcggatt gcgcgcgccg gccatgatcg ctttcgtcaa
     3121 agacatcatg atctatgtct ttgtgcttgc ggcaattgtc atcgtaccga tgaagctcgg
     3181 cggctatggc gcgatcttcg atgcagcggg agcggccctt gccaagaaag ccgcgctcag
     3241 cggcggcaag atcgccgcgg gcctgctgct ttcgccgaag caaatgttac ccttcatcac
     3301 gcttgccatt ggttcaggcc tcgcgctgct gatgtatccc cattcgatga ccggaatgct
     3361 ctcggcccga agtggcaaca cgatccgcat caatgccgtg gcacttcccg cctattcggt
     3421 gatgctcgcg ctgatcgctc tgatgggcta tatggcgctg gcggcgggcg tgcatgtcac
     3481 caatccgcaa gatgccgtgc cgcatctgat cctcgctgtc ttccccgatt ggtttgtcgg
     3541 attttgcttc gctgcgatcg ccatcggcgc gcttgttccc gccgcaatca tgtcgatcgg
     3601 cgcggccaat acattcacgc gcaatatctg gaagccgttc attcatccgg cgatgaattc
     3661 gcaggaagag gccgtgctcg ccaagttgat gtcgctgatc gtcaaggtcg gggcgcttgc
     3721 tttcatcctc ttcgtgccaa cgaaattcgc gctcgatctg caattgctcg gtggcatgtg
     3781 gatggcgcag gtcttcccca ccgtcatttt tgggctttat acgcgctggt tcaacggctg
     3841 ggcgcttctc gccggctggg cgaccggcat ggtgctcggc acctggctcg catggacgcc
     3901 ggcggcctgg gtgccagtgc atgctatctt cggctcaaat ctcaacgtct ataacggcgt
     3961 caccgccttc gtcgcgaacg tattggtcgc ggcggtgctt tcggctttgt tgccaaacac
     4021 agcgcgcgac gagacgcgcg ccgaagatta tgccgacttg ccggaaacag cggtcgctaa
     4081 atagtcacgt cagcagcccg tcgtcggccc ggtcgcgtcg ccgcaccgca tggcggaaaa
     4141 atcattcaaa tgttgcggct gttgtgcgga ggctgatgtc gatgcggacg gcgacggaag
     4201 cccgagcggc ccacatccgg caagcccaag cgtgagaagt gcaagcgcga tctttttgag
     4261 cattccgcac ccccggttaa atttattgtg attggggagt atggccgacc ggcggtgccg
     4321 tcgctagggc ttcaaagcca cactgcgggg cagcgcatat ggcgaaaaga gggcgtggac
     4381 gacggtcgaa cgccctcttt tatgttttag tgccccgccg acccgcttgc gccaattcct
     4441 gtctgcgccc gtacgaattg cgcatcgaag ccagccttat ccttggccgc tctcgcactg
     4501 ttgtcgagct tcgagaagac gtagatgccg atgaaggcga tcgtactcga gaacagggac
     4561 ggatggggat agggaaaagg cg
//
LOCUS       DASWOG010000345         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_99886,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000345 DASWOG010000000
VERSION     DASWOG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99886"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB93_07245"
     CDS             <1..435
                     /locus_tag="VGB93_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY0145932.1"
                     /translation="IASDFNLVAWTGGVDFANYQAQPVLGYYVPREGLSLNSRYQLTP
                     HYFIQGNVTFDMSRQYYPTSLTGNVNQGPWTVASEGLGAGYTDECTTLLVNYSDIYQD
                     IGTGSLVHNQIFMVQLQLRTLGDAKYSSTTYANGAQPLDGVR"
     gene            580..1455
                     /locus_tag="VGB93_07250"
     CDS             580..1455
                     /locus_tag="VGB93_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145933.1"
                     /translation="MIAVSCFFYAPHANAQAIIATVNGAPITNWDVTEREKLLRAMHM
                     PASYDDALNSMIDDQLKLGETDKFKIKATDSEIGQQIALEAGAMNMAPAALMGAIQRA
                     GVSTQHIKDHFGADFEFNLMVQAYNKGVDASESAIRAEMAKDGGKSAAGIDYKLHQVI
                     FTLHGGNVFAETQQKIQAANALRERFTSCAEGLALARNMDDVAVKDEIIRNSLQISDQ
                     LKDILDKTPVGHLTSPERTSDGVEMIAVCSKGVSNDDTAVRKAISERLLSAEMRADAA
                     RRLAELRSYAVIVKH"
BASE COUNT          348 a          479 c          449 g          316 t
ORIGIN      
        1 attgcgtcgg acttcaacct agttgcatgg accggcggcg tcgatttcgc caattatcag
       61 gcgcagcctg tcctcggcta ttacgtcccc cgcgagggtc tgagcctgaa ttcgcgctac
      121 cagcttacgc cgcactattt catccagggc aacgtcacct tcgacatgag ccggcaatac
      181 tatccgacca gtttgaccgg gaacgttaac cagggtccct ggaccgtcgc ttcggagggc
      241 ctcggcgccg gctataccga cgaatgtacg accctgctgg ttaattacag cgacatctac
      301 caggacatcg gcaccgggag cttggtgcac aaccagattt tcatggtaca gcttcagctg
      361 cgcacgctgg gcgatgcaaa gtatagctct actacttatg ccaatggcgc gcagccactt
      421 gatggggtca gataattgcc gttttgcgtt gctcagatca tgattcgtaa aatcactttg
      481 gcgcggaacc agaggccagg gagaaaggcc ccgatgtcta cttgcatcag ctcgaatggg
      541 ccttttttta aggttttggc aggttggggc gcgacggcaa tgatcgccgt cagttgcttt
      601 ttttacgcgc cacacgcgaa tgcgcaggcc atcatcgcga ccgtgaatgg cgctccgatc
      661 acgaactggg acgtgaccga gcgtgaaaag ctgctgcgcg cgatgcatat gccggcctca
      721 tacgacgacg ccctcaactc tatgatcgac gatcagttga agctcggcga aaccgataaa
      781 ttcaagatca aagcgacaga cagcgagatc ggccagcaga tcgccctcga ggccggcgcc
      841 atgaatatgg cgccggccgc cctgatgggc gctattcagc gcgcgggtgt ttcgacgcag
      901 catatcaaag atcatttcgg tgccgacttc gaattcaatc tcatggtgca ggcctataac
      961 aaaggcgtcg atgccagtga gagcgccatc cgcgccgaaa tggccaagga cggcggcaag
     1021 tctgcggccg gcatcgacta caaattgcac caagtgatct tcactctgca tggcggcaac
     1081 gtcttcgcgg aaacacaaca gaaaatccaa gcggccaatg cgttgcgcga gcgcttcacg
     1141 agctgtgctg aaggtttggc gctggcgcgc aatatggatg acgtcgccgt caaggatgag
     1201 atcatccgca actccttgca gatcagcgat cagctcaaag acatattgga taagaccccc
     1261 gtcggtcacc tcacgtcgcc tgagcgcacg tcggacggcg tcgaaatgat cgctgtctgc
     1321 agcaagggcg tctcgaacga cgataccgct gtgcgcaaag caatttccga acgcctgctc
     1381 agcgccgaga tgcgcgccga cgccgcgcgg cggctggcgg agttacgatc ctacgccgtc
     1441 atcgtcaaac actgatgggc gcaggcctca tcgcgctcac ccgcggcgat ccgtcaggca
     1501 tcggcccgga actcgcgctg aaggcttggg cggcaatcca cacctatccc ggagcgccgg
     1561 cctttttcgt catcgccgac cccgatcacc tt
//
LOCUS       DASWOG010000346         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_228288,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000346 DASWOG010000000
VERSION     DASWOG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228288"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..367
                     /locus_tag="VGB93_07255"
     CDS             56..367
                     /locus_tag="VGB93_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0145934.1"
                     /translation="MTDTSQPVPAADDGTLVREIEIINRKGLHARATAKFVQCVEQFV
                     AEVTVSRAGETVGGTSIMGILTLGAGIGSSIVVTAKGQEAEEVLDALATLIGNRFGED
                     E"
     gene            376..651
                     /locus_tag="VGB93_07260"
     CDS             376..651
                     /locus_tag="VGB93_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY0145935.1"
                     /translation="MRQSLHRLVVETQGQGLVEITAAIKERLVHVDIDEGPDDMPAHI
                     RAALTQTQLSIPMRDGELLLGAWQGIYLFEHRRAPHRREIVLHVIGE"
     gene            complement(654..1367)
                     /gene="trmD"
                     /locus_tag="VGB93_07265"
     CDS             complement(654..1367)
                     /gene="trmD"
                     /locus_tag="VGB93_07265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0145936.1"
                     /translation="MWRATIFTLFPEMFPGSLGLSLAGDALKRGVWQLEAIDIRTHGL
                     GKHRNVDDTPAGGGAGMVMRADVLAASLDAALTPDDHRPRLLMSPRGKPLTQARVRDL
                     AAGPGAAIICGRFEGVDERLIEARNLEEISVGDYVLSGGEPAALVLLDAVVRLLPGVM
                     GKEESGAEESFENGLLEYPHYTKPRVFEGREIPEILLSGDHGKIARWREAQARALTEA
                     RRPDLLKPPSLRAKRSDPD"
     gene            complement(1367..1939)
                     /gene="rimM"
                     /locus_tag="VGB93_07270"
     CDS             complement(1367..1939)
                     /gene="rimM"
                     /locus_tag="VGB93_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199116.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0145937.1"
                     /translation="MADKSALPPHIQDLVLVGRIGAAHGIRGEVRLVSFTENPKAIAD
                     HGPLSDASGERHFQIAALRSVKDNIFVARLLGVSTRDAAEALNNLDLYVPRATLPVAG
                     EEEFYHADLLGLRAMDGAGAEIGRVLNVLNFGGGDILEIVPLDGDETLLLPFTKACVP
                     TIDLAQKRLVVVLPVEVEATPEDDDPDKSN"
     gene            complement(1942..2307)
                     /gene="rpsP"
                     /locus_tag="VGB93_07275"
     CDS             complement(1942..2307)
                     /gene="rpsP"
                     /locus_tag="VGB93_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0145938.1"
                     /translation="MSLKIRLARGGAKKRPFYRVVVADSRMPRDGRFIEKLGTFDPVK
                     PKDAADRLVLDTEKAKAWIAKGAQPTDRVARLLDGLGVVKREAQNNPQKAQPKKKAQE
                     RAAAAAAKAGTASEEAPAA"
     gene            complement(2335..>3288)
                     /locus_tag="VGB93_07280"
     CDS             complement(2335..>3288)
                     /locus_tag="VGB93_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0145939.1"
                     /translation="EMVEIKRAANPHEILLVADSLAGQDAVNLAKNFDDRVGITGIVL
                     TRVDGDGRGGAALSMRAVTGKPIKLLGTGEKMDALEDFHPDRIANRILGMGDIISLVE
                     KAAQTIDAQKAQKIADRMRKGKFDLEDLADQLAQVEKIGGIGGIMGMLPGMAKMKDQI
                     ASANLDEKVIKRQRAIILSMTQQERRNPDILKASRKKRIAAGSGTRVEDVNRLLKQHR
                     GIADMMKQMGNAKRGPLSQMASAFGLGGGMPVPSPEQIAELQKQAAGAGGMPKIPELP
                     PGLLNNPSKNLPGLPGLGGAKLPGGLPGLGGGGFNPFGKKK"
BASE COUNT          584 a         1039 c         1013 g          652 t
ORIGIN      
        1 cgcaggacgc cggccgcaaa tatatctacg tcgcaagtcg ggtgctcggc gggaaatgac
       61 cgatacatcg caacctgtcc ccgccgccga tgacggcacg ctcgtgcgcg aaatcgagat
      121 catcaatcgc aagggcttgc atgcgcgcgc cacagcgaaa ttcgtccaat gcgtcgagca
      181 attcgtggct gaagtcacgg tgagccgcgc cggtgagacg gtgggcggca catcgatcat
      241 gggcattctg acgcttggcg caggcattgg ctcatccatc gtcgtcactg cgaaggggca
      301 ggaggcggag gaagtgcttg atgctctagc cactttgatc ggcaacaggt tcggcgaaga
      361 cgagtaatgt ccggcatgcg gcaatcgttg caccgcctcg tcgtcgagac gcagggtcaa
      421 ggtcttgtcg agatcaccgc cgcgatcaaa gaacggctcg tccatgtcga catcgatgaa
      481 ggaccggacg atatgccggc gcatatccgc gcggcgctga cgcagacgca gctttcaatt
      541 cccatgcgcg acggtgaatt gctgctcggc gcatggcagg gcatctatct cttcgagcat
      601 cgccgcgctc cgcaccggcg cgagatcgtg ctgcatgtga tcggcgagtg acgtcagtct
      661 ggatcgcttc gcttcgcccg caatgacggc ggcttgagca aatccggccg ccgcgcttcg
      721 gtcagcgcgc gcgcttgagc ctcacgccaa cgggcgatct tgccatgatc gccggacagc
      781 agaatttcgg gaatttcacg gccctcgaag acgcgtggct tggtgtaatg cggatattca
      841 agcaggccgt tctcgaagct ttcttccgca cccgattctt ccttgcccat gacgccgggc
      901 agcaagcgca caaccgcatc gagcagtacc agcgccgcgg gctcgccgcc cgagagcacg
      961 taatcgccaa cggagatctc ttccagattg cgcgcttcga tcagccgctc gtcgacaccc
     1021 tcaaagcgcc cgcaaatgat cgcggcgcca ggcccagcgg ccaagtcgcg gacgcgcgcc
     1081 tgcgtcagcg gttttccgcg tgggctcatg agcagccgtg ggcggtgatc atccggcgtc
     1141 agcgccgcat cgaggcttgc ggcgagcaca tcggcgcgca tcaccatgcc ggcgccacca
     1201 ccggccggcg tgtcatcgac attgcgatgt ttgccgagcc catgcgtgcg gatgtcgatc
     1261 gcttcaagct gccagacgcc gcgcttcaac gcgtcgccgg ccaacgacag acccagcgat
     1321 cccggaaaca tctcaggaaa gagcgtgaag atggtcgcgc gccacatcag ttcgacttgt
     1381 ccggatcgtc gtcttcgggt gttgcttcga cttcgaccgg taggacgacg acgagccgct
     1441 tttgcgcgag atcgattgtc ggcacgcagg ctttcgtgaa tggcagcagc agcgtttcgt
     1501 cgccgtccaa aggcacaatt tcgagaatat cgccgccgcc gaaattgagc acgttcagca
     1561 cacggccgat ctcggccccc gcgccatcca tggccctcag gccgagaaga tcggcgtgat
     1621 agaattcctc ttcacccgcc accggcaacg tcgcgcgcgg cacgtaaagg tccagattgt
     1681 tcagcgcctc ggccgcgtcg cgtgttgaaa cgccgagaag acgcgcgacg aaaatattat
     1741 ccttcaccga acgcaatgcg gcgatctgga aatgccgctc gccgctggca tcgctcagcg
     1801 gtccatggtc ggcgatggcc ttgggatttt cggtaaagga gacgaggcgc acctcgcctc
     1861 tgataccgtg cgccgcaccg atccgcccga caagcacgag gtcttgtatg tggggcggaa
     1921 gggcgctttt gtctgccatt cttaggccgc aggggcttct tcgctggccg ttccggcctt
     1981 ggcagcagcg gccgcggcgc gctcttgcgc cttcttcttc ggctgcgctt tctgcggatt
     2041 gttctgggct tcgcgcttta cgacaccgag cccgtcgagc agacgcgcaa cgcggtccgt
     2101 cggctgtgcg cccttggcga tccaggcctt tgccttctcg gtatcgagca cgaggcgatc
     2161 ggcggcgtcc ttgggcttca ccggatcgaa cgtgccgagc ttctcgatga agcggccatc
     2221 acgcggcatg cgcgaatccg cgacgacgac gcgatagaag gggcgcttct tggcgccacc
     2281 acgggcgaga cgaattttca gagacattgt tttcctttct gacgaacgat ctggttattt
     2341 cttctttcca aatggattga atccgccgcc gccgagaccg ggcaaaccgc ccggcaattt
     2401 agcgccgcca agacccggca gaccgggcag attctttgac ggattgttca gcaggcccgg
     2461 cggaagctca ggtattttcg gcataccgcc agcgccggcg gcctgtttct gcaattcggc
     2521 gatctgttcc ggcgatggca ctggcatacc gccgccaaga ccaaaagcag acgccatctg
     2581 cgacagcggt ccgcgcttgg cgttgcccat ctgcttcatc atgtcggcga tgccgcgatg
     2641 ctgtttcagc aaccggttga cgtcctcgac gcgagtgccg gagccggcag cgatgcgttt
     2701 cttgcgcgag gccttgagaa tgtcggggtt gcgccgttcc tgctgcgtca tcgacaggat
     2761 gatcgcgcgc tgacgcttga tgactttctc gtcgagattg gccgacgcga tctggtcctt
     2821 catcttcgcc atgccgggca gcatgcccat gatgccgccg atgccgccga tcttctcgac
     2881 ctgcgccaat tgatcggcga gatcttcgag atcgaatttg cccttgcgca tccggtcggc
     2941 gatcttctgc gctttctgcg cgtcgatcgt ctgcgccgct ttttcgacga gcgagatgat
     3001 gtcgcccatg ccgagaatgc gattggcgat acggtcggga tggaaatcct cgagcgcatc
     3061 cattttttcg ccggtgccga gaagcttgat cggcttgccg gtgacagcac gcatcgagag
     3121 cgccgcgccg ccgcggccgt cgccatcgac gcgggtcagc acgatgcccg tgatcccgac
     3181 gcggtcatcg aagttcttgg cgagattgac ggcgtcttga ccagccaggc tgtcggcgac
     3241 gagcaggatt tcgtgcggat tggcggcgcg cttgatctcg accatctc
//
LOCUS       DASWOG010000347         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_337774,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000347 DASWOG010000000
VERSION     DASWOG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337774"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGB93_07285"
     CDS             <1..365
                     /locus_tag="VGB93_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY0145940.1"
                     /translation="INPEVVGGKAKLIEIWERFPKFILGFILTFALGLVVALTLGKSH
                     AAALKSVADEASVFRVTFFVLTFFSIGVLSNFRRLWADGLGKLAAVYFVSLFGFVIWV
                     GLFISWIFFHGVKPPLAS"
     gene            391..540
                     /locus_tag="VGB93_07290"
     CDS             391..540
                     /locus_tag="VGB93_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145941.1"
                     /translation="MSQIDLKTEVQGAQTEPLLPIEKKLIGISLGIGVTLLVILIIVN
                     FLGLL"
     gene            829..1335
                     /locus_tag="VGB93_07295"
     CDS             829..1335
                     /locus_tag="VGB93_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145942.1"
                     /translation="MAETLITIRDAREADAEAIAEVHDAAWRHAYRGIIPGRELERMI
                     ARRGPTWWRSAIQRRSRLVVLDFDDAIGGYASYGRNRVPAMNFGGEIFELYIAPEFQG
                     LGFGKRLFNAARRDLAEHGYSSFLVWALADNEPALSFYGHLGGTLVRRAFERFGSEQR
                     ERVAFAFQ"
     gene            1404..>1554
                     /locus_tag="VGB93_07300"
     CDS             1404..>1554
                     /locus_tag="VGB93_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0145943.1"
                     /translation="MNLDAVPAGKNPPDDINVVIEVPIGGEPIKYEMDKASGALFVDR
                     FLYTAM"
BASE COUNT          301 a          483 c          439 g          331 t
ORIGIN      
        1 atatcaatcc agaggtggtg ggcggcaagg ccaagctcat cgaaatctgg gagcgtttcc
       61 caaaattcat tttgggcttc atcctgacct tcgcgctcgg cctcgtcgtg gcgctgaccc
      121 tcggcaagag ccatgcggcg gcgctcaaat ccgtcgctga cgaggcgagc gtcttccgcg
      181 tcaccttctt tgttctgacg tttttctcga tcggggtttt gtcgaacttc cgccgcctct
      241 gggccgacgg actcggcaag ctcgcggcgg tctatttcgt cagcctgttc ggcttcgtca
      301 tctgggtcgg cctgttcatc tcgtggattt tcttccacgg cgtgaagccg ccgctcgcca
      361 gctgaaccca aatcacttca ggagattaaa atgtcgcaga ttgatctgaa gaccgaagtg
      421 caaggcgccc agaccgagcc gctgctgccc atcgaaaaga agttaatcgg gataagcctc
      481 ggcatcggcg tcaccctatt ggtgattctg atcatcgtga acttcctcgg cctcttgtaa
      541 atttaggcct cttataaaat cccgcgccaa aatagcgcac caacgtgcgg ccgggacgcc
      601 tcagacgggc atcccggccg tactgtcatc gtcgcgacac gcttgcttaa aattgttggg
      661 ccagctctgg cccctgcgac tcacagagga ccgcgcaaag ccctgatgca acgcgcaaat
      721 tccggcaaat tcaactttcc gaatctcgtt cgtaagtccg agccgcgcac gcccaacgcc
      781 aagaagaagt tcttgcaggc cgagccgaag cgggcctaac atctgctcat ggccgagacg
      841 ctcatcacca tccgggacgc tcgcgaagct gatgccgaag caatcgctga ggtgcatgac
      901 gcagcgtggc ggcatgccta ccgcggcatc attccggggc gtgaactcga acggatgatc
      961 gcacggcgtg ggccgacctg gtggcgctcc gccattcaac ggcgatcccg cctcgtggtg
     1021 ctcgacttcg acgacgcgat cggaggctat gcgagctacg ggcgcaatcg cgttccagcg
     1081 atgaatttcg gcggcgagat tttcgagctc tatatcgcgc cggaatttca gggcctgggc
     1141 tttggcaagc ggctgttcaa cgctgcgcgc agggaccttg ccgaacatgg ttactcgtca
     1201 ttcctcgtgt gggccctcgc cgataacgag ccggcgctca gtttctacgg ccatctcggc
     1261 ggcactttgg tgcgccgcgc cttcgaacgg ttcggcagcg agcagcgcga gcgcgtcgcc
     1321 ttcgctttcc aatagcggca tgatcgggta ttgagcggcg ccccttacgc gcagacgcgc
     1381 aattcccaat tataggatca gctatgaatc tcgatgccgt tcccgccggc aagaacccgc
     1441 ctgacgacat caacgtcgtc atcgaagtgc cgatcggcgg cgaacccatc aaatacgaaa
     1501 tggataaggc gtccggcgca ctcttcgtcg atcgctttct ctatacggct atgc
//
LOCUS       DASWOG010000348         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_44314,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000348 DASWOG010000000
VERSION     DASWOG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44314"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VGB93_07305"
     CDS             complement(<1..521)
                     /locus_tag="VGB93_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY0145944.1"
                     /translation="MSSKSSPQSKSSETVPPALATPSGFGANAVRDIAAAINLALADV
                     FALYLKTKNFHWHMSGPHFRDYHLLLDEQGEQIFAMTDVLAERVRKVGGVTLHSIGEI
                     SRNQRIFDNDAPFVTPQDMLAELREDNATLAKSFRAAHEVADEHGDVATTSQLEVYID
                     ETERRTWFLNEASR"
     gene            complement(617..1756)
                     /locus_tag="VGB93_07310"
     CDS             complement(617..1756)
                     /locus_tag="VGB93_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0145945.1"
                     /translation="MNRARTWLSARLSERRAQLRLCLRVTVAAVLTFYISQLLHLPWA
                     LWAVLTAVIMTQTSVGKSLIATIDYMIGTLGGGIYSGLVGIFVPHNSNLEIMLALGLA
                     LAPLAFLASLSPRSAAAPFTAVMVLLLPTITHASALQSAFFRVIEVAVGCAVSLAVSF
                     LVFPQRAHNLVYDGAAKILIRLADILPVLVADLNAPQADTPAHRLQRHVGDAFQKLAA
                     INIEAARERSAYLHSDPDPQPLIDVTLRLRHDIVLLGRAATEPLPPFILERLAQPIEQ
                     FAVAAREFLISAAAALNARAEPAPITQIDAAFANFSEAFAALRRAQLLQNLPTDTVER
                     IFALGFCFEQMHTDLATLGASVGKIARMPKSSEAKKPDAKLEEIA"
     gene            complement(1784..>2238)
                     /locus_tag="VGB93_07315"
     CDS             complement(1784..>2238)
                     /locus_tag="VGB93_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY0145946.1"
                     /translation="VATLTFTIFFGWLLLISGVVGLVSTFSLRPAAGFWWSLLSAILA
                     IAVGFLLIARPGQGAVSLTLLLIVFFIIEGFSSIIFATEYRQNLSGRWGWMVASGVVD
                     LLLAGMIFLQLPSSAIWAIGLLLGINMVFGGTTLVMLALDAKKILPPT"
BASE COUNT          469 a          628 c          730 g          411 t
ORIGIN      
        1 cggctggctt cgttgaggaa ccaggtccgg cgttccgtct cgtcgatgta gacttcgagc
       61 tggctcgtcg tcgcgacatc gccgtgttcg tcggcgactt catgtgccgc tcggaaagac
      121 ttggcgagcg tggcattgtc ctcgcgcagt tcggccagca tgtcctgcgg ggtgacgaag
      181 ggcgcgtcat tgtcgaagat gcgctggttg cgggagatct cgccgatcga atgcagcgtc
      241 acgccgccga ctttgcgaac gcgttcggca agcacatcgg tcattgcgaa aatctgctct
      301 ccttgctcgt cgagcagaag atgatagtcg cggaaatgcg gtccgctcat gtgccaatga
      361 aaatttttgg tcttcagata gagcgcgaaa acatcggcga gcgcgaggtt aatcgcggcg
      421 gcgatatcgc gaaccgcatt cgcgccgaac cccgacgggg tcgccaaagc gggcggcaca
      481 gtttctgagc tcttgctctg cgggctcgat ttgctggaca ttcctctact cctctttggc
      541 acaatgtggc gagcgacatg cggccggcca tcatgaactc aattccgggc tacagaagcc
      601 cggaccgcat ttggttctaa gcgatttctt cgagtttcgc gtcgggtttt tttgcctcac
      661 ttgattttgg catccgcgcg attttgccga cgctggcccc gagcgtcgca agatcggtat
      721 gcatctgttc gaagcagaaa cccagggcga aaatgcgctc gaccgtgtct gtcggtagat
      781 tttgcaataa ttgcgcgcgg cgcagggcgg cgaatgcttc ggagaaattc gcaaaggccg
      841 catcgatttg cgttattggc gcaggctcgg cgcgcgcgtt gagcgcggcg gcggcgctga
      901 tcaggaattc ccgcgccgcg accgcgaatt gttcgatcgg ctgggcgaga cgttcaagaa
      961 tgaacggcgg cagaggctcg gtcgccgcac gtcccagcaa gacaatatcg tgacgcagcc
     1021 gtagcgtcac gtcgatgagc ggttgcgggt cggggtcgga atggagatag gcggagcgtt
     1081 cgcgggctgc ctcgatattg atcgcagcga gcttttgaaa cgcgtcgccg acatggcgtt
     1141 gcagtcgatg cgccggcgtg tcggcttgcg gcgcgttgag atcggcgacg aggaccggca
     1201 aaatatccgc caaccggatc aggattttcg ccgccccatc atagacgagg ttgtgcgccc
     1261 gctgcgggaa aacgaggaat gagacggcga gcgataccgc gcacccgact gcaacttcga
     1321 tgacgcggaa gaaggccgat tgcagtgcgc ttgcgtgcgt gatcgtcggc agaagtaaaa
     1381 ccatgaccgc ggtgaaaggc gccgccgcgg aacgcgggct taaactcgcg aggaaggcga
     1441 gtggggcaag cgccaggccc agcgcgagca tgatttcgag attgctatta tgcgggacga
     1501 atatgcccac gaggccacta tagatgccgc cgccgagcgt gccgatcatg taatcgatcg
     1561 tcgcgatcag cgattttccg acgctcgtct gcgtcatgat caccgcggtc agcaccgccc
     1621 aaagcgccca gggcagatga agcagttgcg agatgtaaaa ggtgagaacg gcggcgaccg
     1681 ttacacgcaa acataagcgc aattgcgccc gccgctcgct caggcgcgcg gacagccacg
     1741 tccgcgcgcg gttcaagaat cccatctttt tttggttctt acattaggtg ggcggcagga
     1801 tcttctttgc gtcgagcgcg agcatgacca atgtcgtacc gccaaagacc atgttgatgc
     1861 cgagcagcag gccgatcgcc cagatcgccg aactcggcaa ttgcaggaag atcatcccgg
     1921 cgaggaggag atcgacgacg ccgctcgcga ccatccagcc ccagcgcccg gagaggttct
     1981 gccgatattc ggtggcgaag ataatcgagg agaagccctc gatgataaaa aagacgatga
     2041 gcagcaaggt gagcgagacc gcgccctggc cgggtcgggc gatgagcagg aagccgaccg
     2101 caatcgcgag gatcgccgag agcagcgacc accagaagcc cgcagcgggc cggagggaga
     2161 acgtggagac gagaccgacc acgccgctga tcagcaggag ccagccaaag aagatggtga
     2221 atgtgagcgt cgcgacga
//
LOCUS       DASWOG010000349         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_246410,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000349 DASWOG010000000
VERSION     DASWOG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246410"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGB93_07320"
     CDS             complement(<1..273)
                     /locus_tag="VGB93_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY0145947.1"
                     /translation="MMTSLDAAHDHHHDHNSEGAGATVIDPVCGMSFDPATAKHHLVH
                     AGHDYFFCSGNCREKFEAAPESYLKPVGPKPAPITHDHVRWTCPMHP"
     gene            363..746
                     /gene="cueR"
                     /locus_tag="VGB93_07325"
     CDS             363..746
                     /gene="cueR"
                     /locus_tag="VGB93_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEY0145948.1"
                     /translation="MNIGQAAAASGVNAKMIRHYESIGLILPAERSESGYRLYSQSDL
                     HVLSFIKRARKLGFSIKDIQRLLALWQEQRPSADVKRVALAHVAELDTRIAELQGMRN
                     TLRHLANKCNGDARPTCPILEDLAG"
     gene            complement(815..1087)
                     /locus_tag="VGB93_07330"
     CDS             complement(815..1087)
                     /locus_tag="VGB93_07330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0145949.1"
                     /translation="MDKVTDNPSRHRFELAVDNEVAFVTYAMEGNRLALLHTEVPKAL
                     EGRGVGKLLIRSVLDEARQRGLKIVPRCEFVSAFIERNPEFKDLIA"
     gene            complement(1137..2834)
                     /gene="oxc"
                     /locus_tag="VGB93_07335"
     CDS             complement(1137..2834)
                     /gene="oxc"
                     /locus_tag="VGB93_07335"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEY0145950.1"
                     /translation="MTGTTPQAEFTDGFHLVIDALKLNGIDTIYGVAGIPITDLARLA
                     QAEGLRYFGFRHEQSAGNAAAIAGYLTKKPGICLTVSAPGFLNGLVALANATTNCFPM
                     ILISGSSDRAIVDLSQGDYEELDQMNAAKPFAKAAFRINRPQDIGIGIARAIHAAVSG
                     RPGGVYLDLTAAVLSATLDVTAAKKSLVKVVDAAPSQIPAPESVARALSLLASAQRPL
                     IILGKGAAYAQADKDIRSFIEATGIPFLPMSMAKGLLPDNHPQSVAAARSFAIGEADV
                     VMLIGARLNWLLSHGKSPLWPENTQFIQLDIAPTEIDSNQPIAAPVVGDIQSSISALL
                     AATLKSGKIHAGADWLKAIEQHKQKNIERMAARLKAEPHPMNFSSALRAVRDVLVNKP
                     EINIVNEGANTLDFARNIIDMYEPRKRLDSGTWGVMGIGMGYAIAAAVETGKPVIAIE
                     GDSAFGFSGMEIETICRYHLPVVIVVLNNGGIYHGDKAGSLPSPTGFIANARYDKLIE
                     AFGGIGYHVTDTAGLTNALISALADGQPTLIDCVIDPTAGTESGHIQNLNPKSALVN"
     gene            complement(2926..4224)
                     /gene="frc"
                     /locus_tag="VGB93_07340"
     CDS             complement(2926..4224)
                     /gene="frc"
                     /locus_tag="VGB93_07340"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY0145951.1"
                     /translation="MSKPLEGIKVIDFAGVQAGPVCAQFLAWYGADVIKVERVEGGDI
                     TRHQLRDIPDVDALYFTMHNSNKRSIALDTKSPEGKEVFEKLLAWADVIVENFRPGAM
                     DRMGFGWERLHEINPRLIWGSIKGFHDNSPFANLSVYENVAQCAGGAASTTGYWNGEP
                     AVSGQASGNNNGPLISAAALGDSNSGYHLLIGILTALFGREKTGRGQKVSVSMQDAVL
                     NLCRVKLRDQQRLERVGYLEEYPQYPNGKFTDVVPRGGNAGGGGQPGWILKCKGWETD
                     PNAYIYFTIQEQNWAATAEAIGKPEWANDPNYTTAKARQSHIFDIFTEIEKFLANKTK
                     YEAIEYLSKYRIPCAPVLSMKEIAEAPDMRETGTIVEVKQPKRGTFLTINPIKFSDFT
                     PEITSAPLLGEHSDEILAQFGYTPAQIKTFHDKKVVATTA"
     gene            complement(4266..5576)
                     /gene="oxlT"
                     /locus_tag="VGB93_07345"
     CDS             complement(4266..5576)
                     /gene="oxlT"
                     /locus_tag="VGB93_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY0145952.1"
                     /translation="MVMAAAPQSGLVRARSERWWYLALCVVCMIMIANFQYGWSVFVS
                     PLHKAHGWSVSNIQLTFTIFVALETWATPLNGWLADRLGPHLGPRVVMGAGGLLVALG
                     WIVNSYATSLPILYLGGALSGLGAGAVYCTAVGTAVKWFTDHRGLAVGLVAGGFGAGA
                     ALTIIPIQMAIKTTGYASAFFWAGLLQGAVVLIVSQFIRNPNRGEVAAQVSAKAQQTT
                     YSYSPHEVLTSRVFWVLYVLDLLMCAGGLIVTAYLAPIADSYHVSDVIVFMGATTLSI
                     ALIFANVMNGIARPLFGWVSDKIGLSETMAIAFALGAIAYFLLSVTGKYPWGLIVFVG
                     MVFFCWGEIFSLFPAMCTDLFGQKYATTNSALLYTAKGAAAFLVPLGGLAASITGNWN
                     DVLFLTTGINVVAVVIVLTVLRPAAARHHATAIIKSEKLAVNTR"
     gene            complement(5631..>6318)
                     /locus_tag="VGB93_07350"
     CDS             complement(5631..>6318)
                     /locus_tag="VGB93_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0145953.1"
                     /translation="DGPPTRVGTSLSDLCAGLFMFGGIVSALFAREKSGRGAHIDVAM
                     FDSTLAFLEHGFMEYVATGKSLGRIGNRHPFMAPFDVFASKDDDFVIAAGNDHLFAAL
                     CEAIGRPDLSRDSRFVSNEGRMENNAALKSELETALKKQPAAHWLKVIHGAGVPVAPI
                     LNVSESAEHPQTQARNMLIEAGGVRMTGNPIKISGYPDPHVREGAPTLNQHGDALRRE
                     LAVPVSADAT"
BASE COUNT         1356 a         1812 c         1812 g         1338 t
ORIGIN      
        1 cggatgcatc gggcatgtcc agcgaacgtg gtcatgcgta atgggcgccg gcttgggccc
       61 cactggcttc agataggatt ccggagcggc ctcaaacttt tcgcggcagt tgccactgca
      121 gaagaaataa tcgtggcccg catggaccag atggtgcttg gcggtcgccg gatcgaagga
      181 catgccgcat accggatcaa tgaccgtggc gccggcccct tcagaattgt gatcgtgatg
      241 gtggtcgtga gccgcatcga gcgaggtcat catttcccgc ctccgtgagt aaaacaggtc
      301 ttgccgctga acatggggct tcccattatt ggaaggtcaa gaggcggagg ctaagaaatt
      361 cgatgaatat cggccaagct gccgctgcga gcggcgtgaa tgcgaagatg atccggcact
      421 atgaaagcat cggcctgatc cttccggctg agcgaagcga gagcggctat cgtctctaca
      481 gccaatctga cctgcatgtt ttgagcttca tcaaacgcgc acggaaactc ggcttttcga
      541 tcaaagatat ccagcggctt ctagcccttt ggcaagagca gcggcctagc gccgacgtca
      601 aaagagtcgc tctcgcacac gttgcagagc tggatacgcg gatcgccgag cttcaaggga
      661 tgagaaatac gctgcgtcac ctcgcaaata agtgcaatgg cgacgcccgt cccacctgtc
      721 ccattctcga agatcttgcc ggctgatatc ggacataaga ggcaagtttg cgcgccgctt
      781 cgcccgggac cgatgatttg ttgccaagat aatttcaggc gatcaggtct ttgaattcag
      841 gatttcgctc gataaaggcc gagacgaact cgcatctcgg cacgatcttc agtccgcgtt
      901 gtcttgcctc atcgagcacc gaacggatca ggagcttgcc gacgccccga ccctccaacg
      961 ctttcggcac ttccgtgtga agcaatgcaa gcctgttgcc ttccatcgca taggtcacaa
     1021 aagcgacctc gttgtcgacg gcgagttcga agcgatgacg acttggattg tccgtgacct
     1081 tgtccatgaa cgactccatt gaagggcgcg tttggccgcc gcgtcacggc ccatcgtcaa
     1141 ttcaccaggg cacttttcgg attgagattt tgaatatggc cgctctcggt gccggcggtc
     1201 ggatcaataa cgcagtcgat gagcgtcggc tgtccgtccg caagggcact gatcaaagca
     1261 tttgtgagac cagccgtatc cgtcacgtga tatccgatgc ctccaaaagc ctcgatcagc
     1321 ttgtcatagc gcgcattggc gataaaaccc gtcggtgaag gaaggcttcc ggctttgtcg
     1381 ccgtgataga tgccgccgtt gttgagcacc acgatcacca ccggcagatg gtaacggcaa
     1441 attgtctcta tttccattcc gctaaaccca aaggcgctgt caccctcgat ggcaatgacc
     1501 ggcttgccgg tttccaccgc agcagcaatc gcatatccca tgcctatacc catgactccc
     1561 cacgttccgc tgtcgaggcg tttgcggggc tcatacatgt cgatgatgtt gcgggcgaaa
     1621 tcgagcgtat tggcgccttc gttcacgata tttatctcgg gcttgttcac cagaacatct
     1681 cggacggcgc gtaacgcgct cgaaaagttc atcggatggg gctcggcttt gagccgggct
     1741 gccatgcgtt caatgttctt ctgcttatgc tgctcgattg ccttgagcca atcggccccg
     1801 gcgtgaatct tccccgactt gagggtcgcg gcaagcaagg ccgagatcga agactgaatg
     1861 tcgccgacaa ccggcgccgc gatcggttgg ttgctgtcga tttcggtcgg cgcaatgtcc
     1921 agctgaataa attgcgtgtt ctcgggccaa agcgggcttt ttccgtggga aagaagccag
     1981 ttcaggcgcg ccccgatcag catcacgacg tcggcctcgc cgatcgcgaa ggatcgtgcc
     2041 gccgcgacgg attgcggatg attgtcgggc agaagtccct tggccatcga catcggcaaa
     2101 aatgggatgc ctgtggcctc gataaaggag cgaatatcct tatcggcctg agcgtaggcc
     2161 gcaccctttc cgagaatgat caggggacgc tgggcactcg ccaggagtga gagcgcgcgc
     2221 gcaaccgatt ccggagctgg aatttgactc ggcgccgcat cgacgacttt taccagcgat
     2281 ttttttgctg ccgtcacgtc gagtgttgcg ctgaggacag ccgcggtcag atcgagataa
     2341 acaccgccgg ggcggcctga aaccgcagca tgaatagcgc gcgcaattcc gataccgata
     2401 tcctgcgggc ggttgatccg aaacgccgct ttagcgaaag gcttggcggc gttcatctga
     2461 tccagttcct cgtaatcgcc ttgcgacaaa tcgacgattg cgcgatcact cgatccgctg
     2521 atgaggatca tcgggaagca attggttgtg gcattcgcca gcgcgaccaa tccgttcagg
     2581 aatcccggtg ctgagaccgt gaggcaaatt cccggcttct tggtgagata gcccgcgatc
     2641 gcggcggcgt tgcccgcgct ttgttcgtgc cgaaatccga aatatcgcaa cccttccgcc
     2701 tgcgccaacc gcgcgaggtc ggtgataggg atgcctgcaa caccgtagat cgtatcaatt
     2761 ccgttcagtt tcaaagcgtc tatgacgaga tggaatccgt cggtgaattc agcctgcggc
     2821 gttgtgcctg tcatgtcttt catccttgtc aaatgatggg cgcccgcgac gtgaagcgaa
     2881 ggagcgccct agctctttga acggcgcagg gtgcgtaaca acgcatcacg ctgtcgtggc
     2941 aacgaccttc ttgtcatgga acgtcttgat ctgcgcgggc gtatagccga attgtgcgag
     3001 gatctcgtcg ctatgctcgc cgagcaatgg cgcgctcgtg atctcgggcg tgaagtcgct
     3061 gaacttgatc ggattaatcg tcaggaaggt gccgcgcttt ggctgcttga cctcgacgat
     3121 cgtcccggtc tcgcgcatat ccggcgcttc ggcgatttct ttcatcgaga gcaccggcgc
     3181 gcagggaatg cgatacttgc tgagatattc gatggcttca tatttggttt tgttggccag
     3241 aaatttttcg atctctgtga aaatatcgaa aatatgcgac tggcgcgcct tggccgtggt
     3301 gtagttcgga tcgttggccc attcgggctt gccgatcgcc tcagcggtcg cagcccaatt
     3361 ctgttcctgg atcgtgaaat agatataggc gttgggatcg gtctcccagc ctttgcattt
     3421 cagtatccag ccaggctgac cgccgccgcc cgcattgcca ccccgaggca cgacatcggt
     3481 gaacttgcca ttcgggtatt gcggatattc ctcgagataa ccaacgcgtt ccaagcgctg
     3541 ctggtcacgc agcttgacgc ggcagagatt gagcaccgca tcctgcatcg agacgctaac
     3601 cttctgaccg cgtccggtct tttcccggcc gaatagggcg gtgaggatgc cgatcagcaa
     3661 atgatagccc gaattgctgt cgccgagcgc cgcggcgctg atcagcgggc cgttgttgtt
     3721 accggatgcc tgccccgata cggccggctc cccattccaa tagccggttg ttgaggcagc
     3781 accgccagcg cattgcgcga cgttttcgta gacgctgaga ttggcgaagg gggaattatc
     3841 atgaaatccc ttaatcgaac cccagatcag ccgtggattg atctcgtgga gacgctccca
     3901 tccgaagccc atccggtcca tagccccggg gcggaaattc tcgacgataa catcggccca
     3961 ggctaacagc ttctcgaaca cctccttgcc ttcaggactc ttagtgtcca acgcgattga
     4021 acgtttgttg ctgttatgca tggtaaagta gagcgcatcg acatcgggga tgtcgcgaag
     4081 ctggtggcgc gtgatatcgc cgccttccac gcgctcgacc ttgatgacat ctgcgccata
     4141 ccacgccaaa aactgggcgc aaacgggacc tgcctgtacg ccagcaaaat caatgacctt
     4201 gatgccttca agtggtttac tcattttcgt aactcctcaa gtcttcatgc gtaattgtgg
     4261 cgcggtcagc gtgtattcac tgccagcttt tcagatttta tgatcgccgt tgcatggtgc
     4321 cgtgccgccg cgggacgcag aaccgtgagg acgataacga cggcgacgac gtttatgccc
     4381 gtcgtcagaa agagcacgtc attccagttt ccagttattg aagcggcaag gcctcctagc
     4441 gggaccaaga acgccgcggc acctttcgcg gtgtagagaa gtgcggaatt tgtcgtggcg
     4501 tatttctgcc caaacagatc cgtgcacatc gccgggaata ggctgaagat ttcaccccaa
     4561 cagaagaaca ccatgccgac aaagacgatg agtccccacg gatatttccc ggtgacgctg
     4621 aggaggaagt aggcgatggc gccgagagca aacgcgatcg ccatggtttc cgagagaccg
     4681 atcttgtcgg acacccatcc aaacagcgga cgcgcaatgc cattcatcac gttggcgaaa
     4741 atgagcgcga tggagagcgt cgtcgctccc atgaatacga tcacatcgga gacgtgatag
     4801 gaatcggcga tgggcgccaa ataagcggtg acgatcaaac caccggcgca catcagcagg
     4861 tccaacacat aaagaaccca aaacacgcgg ctcgtcagga cctcatgcgg cgaatagcta
     4921 tatgtggtct gctgcgcctt cgcggaaact tgcgccgcga cttcgccgcg atttggattc
     4981 cgaatgaact gcgacacgat cagaacaacg gcgccctgga gcaatccagc ccagaagaac
     5041 gccgatgcgt agccggttgt tttgatggcc atttggatgg gaattatcgt cagcgcagcg
     5101 ccggcaccga agccgccggc aacaagaccg accgccaggc cgcgatgatc ggtgaaccat
     5161 ttcaccgctg ttccgactgc tgtgcaatac acggcacccg cgccgagacc ggacagagcg
     5221 ccaccgagat acaggatcgg caatgacgtg gcgtaggaat tgacgatcca gccaagcgcg
     5281 accaagagac cgcccgcacc catgacgacg cgcggtccga gatgtggacc aagccgatcg
     5341 gccagccatc cgttgagcgg cgttgcccac gtctccagag cgacaaaaat cgtgaacgtc
     5401 agctggatgt tggaaaccga ccacccatgc gccttatgca aaggtgatac gaagaccgac
     5461 caaccatatt ggaaattggc gatcatgatc atgcaaacga cgcaaagcgc gaggtaccac
     5521 catcgctcac tgcgcgcgcg cactaggccc gattgtgggg ctgcggccat aaccatctga
     5581 aacactcctt cgaactcagt tgcgtatctt cggaagattt ggtcgggcgc ttacgtcgcg
     5641 tccgccgaaa ctggcaccgc gagctcacga cgcagcgcgt cgccgtgctg attgagcgtc
     5701 ggggcgcctt cgcgcacgtg cgggtctgga tatccggata tttttatggg gttgcccgtc
     5761 attctgaccc cgcccgcttc aatcagcatg ttgcgtgctt gagtctgagg atgttcggcg
     5821 ctttccgaga cgttgaggat cggagcgact ggaacccctg cgccgtgaat gaccttcagc
     5881 caatgcgccg ctggctgttt tttaagagct gtttcaagtt cggatttgag ggcggcattg
     5941 ttctccatac gtccttcgtt ggagacaaac cggctatcgc gcgaaagatc ggggcgaccg
     6001 atcgcctcgc aaagcgccgc aaagaggtga tcgtttccag ctgcgatgac aaagtcatca
     6061 tccttcgatg cgaaaacatc gaacggcgcc atgaaaggat gacggttgcc gattcgccca
     6121 agcgacttac ccgtcgcgac atattccatg aagccgtgtt cgagaaacgc gagcgtcgaa
     6181 tcgaacatag caacgtcgat atgcgcgcct ctgccggatt tctcgcgggc aaaaagcgcg
     6241 cttacgattc cgccgaacat gaagagcccg gcgcagagat cggagagcga cgtcccgacg
     6301 cgcgtcggcg gcccgtct
//
LOCUS       DASWOG010000350         7141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_191864,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000350 DASWOG010000000
VERSION     DASWOG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191864"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VGB93_07355"
     CDS             <1..972
                     /locus_tag="VGB93_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0145954.1"
                     /translation="HRISLGMKQLLDDPWQGIDAKYPVNARIHGRVTNITDYGAFVEL
                     EPGIEGLIHVSEMSWTKKNVHPGKIVATSQEVDVQVLEVDPVKRRISLGLKQTLANPW
                     EDFAEKHPVGSIVEGEVKNKTEFGLFVGLEGDVDGMVHLSDLDWNKPGEQVIDDYKKG
                     DIVRAKVLDVDQEKERISLGVKQLGTDPFDAPATEEGGPDFKKGSIVTCEVIDVKEGG
                     IDVKITGTDLVAFIKRSELARDRADQRPDRFAICEKVDVRITQLDRRARKVAVSIKAL
                     EVAEEKEAIAQFGSADSGAVLGDILGAALKARDTDTKKGAKKKEDDE"
     gene            1055..2371
                     /locus_tag="VGB93_07360"
     CDS             1055..2371
                     /locus_tag="VGB93_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0145955.1"
                     /translation="MNIADGVAIGVDFGTTNSVVAIAGRDGEVETLTWPSAAGPTQTF
                     RTALMFWREGRAVKHVAGPEALQRAIAAEGEQRFIQSIKTHLASPLFSETRLYGERFT
                     IERLVSTFLGHLLGDTRDLAQSLPVVSGRPVVFAGERPDEDLAVTRLTQSYRDAGLAQ
                     VRLEYEPLGAAYWYARRLQRDETVLVADFGGGTSDFSVLRFERQGASFVAHPLAHTGV
                     GVAGDTFDYRIITNVVAPRLGKGTSYRSFDKLLPIPAYFYAAFAQWHQLSWLKSGATL
                     AELRKLISVAETPRMLENLQILIEMDLGFELYRSVSDVKAALSSADAVPFRFDREGVK
                     IEAEVTRRDFELWIAPDLAKLAEALERAIMRAGLRSEDIDAVFLTGGTSFVPAVRALF
                     QSRFGEARVHIGDAFQSVAAGLALMALDQMQNNVPTRGLASSSTGA"
     gene            2407..3384
                     /gene="sppA"
                     /locus_tag="VGB93_07365"
     CDS             2407..3384
                     /gene="sppA"
                     /locus_tag="VGB93_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0145956.1"
                     /translation="MSNTSPADYIVDRRLLQRKLSFWRVVSILLLIAGLVFIGLRVSG
                     RSSLGLASLHIARITISGVITGDRATLKLINDVAESKNTAGVILAIDSPGGTTTGAEK
                     LFIALRRLAAKKPTVAVVGTMAASGAYIAAIGADHIVAPGNALVGSIGVLFEYPDLSG
                     LLGKVGVKMDSVKSSPLKAEPSGYEPTSPEVRAALASLVADSFTWFKGLVKDRRGMTD
                     AQLATVDDGRVFTGRQGIGLKLIDEVGEEREGVAWLERVKKVPHGLPVRDWTATDRFQ
                     RFGIFSSAAKIAEVLGFPGLANVLGGTETYAQGQLLDGLVAIWQAGPAN"
     gene            3524..3814
                     /locus_tag="VGB93_07370"
     CDS             3524..3814
                     /locus_tag="VGB93_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY0145957.1"
                     /translation="MIKSELVQRIADRNPHLYLRDVEKIVNAILDEIVKALARGDRVE
                     LRGFGAFSVKHRDARLGRNPRTGAHVSVDEKAVPFFKTGKEMRERLNDGYLS"
     gene            3834..4118
                     /locus_tag="VGB93_07375"
     CDS             3834..4118
                     /locus_tag="VGB93_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY0145958.1"
                     /translation="MKRLLEVLILLPLAIIGVALAVANRHAVLVSFDPFTSTTAGAVE
                     APLFIVLIAAIIIGVVLGGFFTWLSQGKHRRALRESRAETARLRSQTGHP"
     gene            4157..4777
                     /locus_tag="VGB93_07380"
                     /pseudo
     CDS             4157..4777
                     /locus_tag="VGB93_07380"
                     /inference="COORDINATES: protein motif:HMM:NF002295.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            4913..6148
                     /gene="trpB"
                     /locus_tag="VGB93_07385"
     CDS             4913..6148
                     /gene="trpB"
                     /locus_tag="VGB93_07385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0145959.1"
                     /translation="MQLENPNSFRTGPDDRGHFGIFGGRFVAETLMPLILDLERHYEL
                     AKKDPAFHAELDNLLTHYVGRPSPLYFAERLTDYLRRKAADAEHGAKIYFKREELNHT
                     GAHKINNVLGQILLARRMGKTRIIAETGAGQHGVATATACARFGLECIVYMGEVDVER
                     QKPNVFRMNMLGAKVVPVQSGARTLKDAMNEALRDWVTNVENTFYCIGTAAGPHPYPA
                     MVRDFQCVIGEETRTQMQAAEGRLPDSLFACIGGGSNAIGLFHPFLDDRQIEIYGVEA
                     AGYGLDKAHAASLAGGRPGVLHGNRTYLLMDADGQIEEGHSISAGLDYPGIGPEHSWL
                     KETGRVTYLSATDSEALAAFQLCAQQEGIIPALEPAHALARVIDIAPQKPRDHLMVVN
                     ISGRGDKDIFTVADHLGGM"
     gene            6177..7058
                     /locus_tag="VGB93_07390"
     CDS             6177..7058
                     /locus_tag="VGB93_07390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0145960.1"
                     /translation="MSSQPIMSDIEGLTKVAPLSRRGFMTAAGAAAAGYTLAAGPVRA
                     DAIHTSAEGLDTGDAKVAVSGGDMPLYFAKPAGAKKPPIILVAMEVFGLHEYIKDVTR
                     RLGKLGTLAVAPDYYFRLGDVSKISELPKLLPIVNSKPDKELFADLDATLAWAMKQGG
                     DPARVGIIGFCRGGRTVWLYSTHNPHLKAGVAFYGSLMDPPSAAMPKNAYQLAGEVKE
                     PVLGLYGAQDQGITPDQVEKMKAALAADHKAAEFHIYPGAPHGFHADYRPSYRKDAAE
                     EGWKRMKAWFEKYGVLP"
BASE COUNT         1331 a         2128 c         2282 g         1400 t
ORIGIN      
        1 caccgcatct cgctcggcat gaagcaattg ctcgacgatc cgtggcaggg catcgacgcc
       61 aaatatccgg tcaatgcccg catccatggc cgcgtgacca acatcaccga ctacggcgcg
      121 ttcgtcgagc tggagccggg catcgaaggc ctgatccacg tttccgaaat gtcgtggacc
      181 aagaagaacg tccaccccgg caaaatcgtc gcgacgagcc aggaagtcga cgttcaggtt
      241 ctcgaagtcg atccggtcaa gcgtcgtatt tcgctcggcc tcaagcagac tctcgccaat
      301 ccgtgggaag attttgccga gaagcatccg gtcggcagca ttgtcgaggg cgaggtcaag
      361 aacaagaccg agttcggtct gttcgtcggc ctcgaaggcg atgtggacgg tatggtccat
      421 ctctccgatc tcgactggaa caagcccggc gagcaggtga tcgacgacta caagaagggc
      481 gatatcgttc gcgccaaggt gctcgatgtc gatcaggaga aggagcgcat ttcgctcggc
      541 gtgaagcagc tcggcactga cccgttcgac gcgcctgcga cggaagaagg cggccccgac
      601 ttcaagaaag gctcgatcgt cacctgcgaa gtgatcgacg tgaaagaagg cgggatcgac
      661 gtcaagatca ccggcaccga tctcgtcgcg ttcatcaagc gcagcgaatt ggcccgcgac
      721 cgtgccgatc agcggcccga ccgtttcgcc atctgcgaaa aggtcgatgt ccgcatcacg
      781 cagctcgatc gccgcgcgcg caaggtcgcg gtttcgatca aggcgctcga agtcgccgaa
      841 gaaaaggaag cgattgccca gttcggttcg gccgattcgg gcgccgtgct tggcgatatt
      901 cttggcgcag cgctgaaagc gcgcgacacg gacaccaaga agggtgccaa gaagaaggaa
      961 gacgacgaat aatttcgtcg agttctcgga attaagagcc cgcgcggtgc aaaccgtgcg
     1021 ggcttttctg tcagtcccct tgatgaatgg tcatatgaat atcgcagacg gcgtcgcgat
     1081 cggcgtggat ttcgggacga cgaattccgt cgtcgcgatc gccgggcggg acggcgaggt
     1141 cgagaccctg acttggccga gcgccgccgg cccgacacag accttccgca ccgccctgat
     1201 gttctggcgc gaaggccgcg cggtgaaaca cgtcgccggg cctgaggctc ttcaacgcgc
     1261 cattgcggcc gagggcgagc agcgcttcat ccagtcgatc aagacccatc tggcgagccc
     1321 gctcttcagc gagacgcggc tctacggcga acgtttcacg atcgaacgcc tggtttcgac
     1381 ctttctcggt catttgctcg gcgacacgcg cgatctcgcg cagagcttgc cggtggtcag
     1441 cgggcggccc gtcgtcttcg ccggcgagcg gccggacgaa gatctggcgg tcacacgtct
     1501 gacgcaatcc tatcgtgatg cggggctggc gcaggtgagg ctggaatacg agccgctcgg
     1561 cgcagcctat tggtatgcgc gccggctgca acgcgacgag acggtgctgg tcgcggattt
     1621 cggcggcggc accagcgatt tctcggtcct gcgcttcgag cgccagggcg cgagtttcgt
     1681 cgcgcatccg ctggcgcata cgggcgtcgg tgttgcgggc gacacgttcg actaccggat
     1741 catcacgaat gtcgtggcgc cgcgccttgg caagggaact tcttatcgct cgttcgataa
     1801 gctcttgccg attccggctt atttctacgc tgccttcgca caatggcatc aattgtcctg
     1861 gctgaagtcg ggtgcgacgc tcgccgaatt gagaaaattg atctcggtgg ccgaaacgcc
     1921 acggatgctt gagaatttgc agatcttgat cgaaatggat cttggatttg agctttatcg
     1981 gagcgtcagc gatgtgaagg ccgcgctttc aagcgccgac gccgtgcctt tccgcttcga
     2041 tcgcgaaggc gtgaaaatcg aggccgaggt gacgcgtcgt gatttcgagc tatggatcgc
     2101 gccggatctt gccaaattgg cggaagcgct ggagcgcgcc atcatgcggg ctggattgcg
     2161 gtccgaggat atcgatgccg tcttcctcac cgggggaacg tcctttgttc ccgccgtccg
     2221 ggctctgttt cagagccggt tcggcgaggc gcgcgttcat atcggggatg cctttcaatc
     2281 ggtcgccgcg ggcctcgctc tgatggcact cgatcaaatg caaaataacg tgccgacgcg
     2341 cgggcttgcc tcatcaagca ctggggctta ggttccggcc cggcttccac cgccaaggga
     2401 acaggcatgt cgaatacatc tcccgccgat tacatcgtcg atcggcggct actgcagcgg
     2461 aaattgtcct tttggcgggt cgtttcgatt ttgctgctga ttgccgggct cgtgttcatc
     2521 ggcctgcgcg tttccggccg ctccagcctc gggcttgcgt cgttgcatat cgcgcgcatc
     2581 acgatctctg gcgtcattac cggggaccgg gcgacgctga agctgatcaa cgatgtcgcg
     2641 gagtccaaaa atactgcagg tgtgatcctc gcgatcgata gcccgggcgg cacgacgacg
     2701 ggcgccgaaa agctcttcat cgcgctgcgg cgtctggcgg cgaaaaagcc gacggttgct
     2761 gtcgtcggca cgatggcggc gtcgggtgcg tatatcgcgg cgattggcgc ggatcacatt
     2821 gtggcgccgg gcaacgcgct cgtcggctcg atcggcgttc tgtttgaata tcccgatctc
     2881 tcgggtctcc tcggcaaggt cggggtgaag atggattcgg tgaaatcctc gccgctcaag
     2941 gcggagccaa gcggctatga accgacgagc ccggaggtgc gtgccgcgct agcttcgctc
     3001 gttgccgaca gcttcacatg gttcaaaggc ctggtgaagg accgccgcgg catgaccgac
     3061 gcgcaattgg cgacggttga tgacggccgc gtgtttaccg gcaggcaggg catcgggctg
     3121 aagctcatcg atgaagtcgg cgaggagcgc gaaggcgtcg cctggctcga gcgcgtcaag
     3181 aaggtgccac atgggctgcc ggtgcgcgac tggaccgcca cagaccggtt tcagcgattt
     3241 ggcattttca gcagcgcggc aaaaatcgcc gaggttttgg gctttccagg gctggccaat
     3301 gtgctgggcg gcacggaaac ttatgcccag ggacaattac ttgacggtct tgtggcgatt
     3361 tggcaggctg gacctgctaa ttgaagccaa acgtgtttcg ggcgttggcg gtcaccttag
     3421 aacatgattt gatgacgcga ggcgcgctct ccggggactg gagtgcgccg ggcaggtttc
     3481 gcttgagggc gaagtctgca gagaatgacg gcagggggcg aacatgatca agtcggaact
     3541 cgtgcagcgg atcgcagacc gcaatccgca tctttatctg cgcgacgttg agaagatcgt
     3601 gaacgccatc ctcgacgaga ttgtcaaagc gctggcacgc ggtgaccggg tggaattgcg
     3661 cggctttggc gctttttcgg tcaagcatcg cgacgcgcgt ctcggccgca atccgcgcac
     3721 cggcgcgcat gtgtccgtcg acgaaaaggc cgtgccgttc ttcaagaccg gcaaggaaat
     3781 gcgcgaacgc ctcaacgacg gctatctcag ctgatcgcag ccgggtcttg cagatgaaac
     3841 gccttctgga agttttgatt ctgctgccgc ttgcgatcat cggggtcgct ctggcggtcg
     3901 cgaaccggca cgcggtcctc gtgtctttcg atcctttcac ctcgaccacg gccggcgcag
     3961 ttgaagcgcc gctgttcatc gttctcatcg ccgcgatcat tatcggcgtc gtgctcggcg
     4021 gctttttcac ctggctgtcg cagggcaagc atcgccgcgc cttgcgcgag agccgcgccg
     4081 agaccgcccg gctgcgaagc caaaccggcc acccgtaaca ctcgtttttg ccgccgcgag
     4141 cgtgtaaaag cctggcatga ttgtcaaaat ctgcgggctt tcgacctccg agacgcttgc
     4201 cgccgccctt aatgccagcg ccgacatggt cggcttcgtt cattacgagc ggagcccgcg
     4261 tcatgtgtcg ctcacggccg cgccggcact tgtcgctcaa gtcgcaggcc gggcgccgaa
     4321 aacattgctt actgtcgatg cggacgacga gcttctcagt gcggcgatca aggctttcgc
     4381 acctgatatt ttgcaattgc atggtgcgga gaccccggcg cgggtcgccg cgatccgcga
     4441 aaaattcggg cgcccggtga tgaaggcgat tggcctcgga acagtcgctg atctcgacaa
     4501 gatcggcgtc tatgaagtag ttgccgacat gctgcttttc gatgcccagc ccactgacgc
     4561 ggcgctgccg ggtggcaatg gccgcgcttt cgattggacc ttgctggccg ggcgcagctt
     4621 cgccaagccg tggctcctcg gcggcggcct gacgggggat aatgtcgcgg cggcgattgc
     4681 cgcgacgggt gcgccgggcg tcgatgtttc ctccggtgtc gaaagcaccc gcggcgtcaa
     4741 ggatattggc aaaatcatcc gtttcatcgc cgcggcgcat gcgacggaaa agcttggttc
     4801 gctggctgag agcggacaga gcatcggacc gaaaagtgcg aagcggtttt cggataaatc
     4861 cgatgcgacg aaacgattaa gagcaggcgc aaatcgagaa cgcgagagca cattgcaact
     4921 ggaaaacccc aactccttcc gtactggccc cgacgaccgc gggcatttcg gcattttcgg
     4981 cggacgtttc gtcgccgaga ccttgatgcc gctcattctc gatctcgaac ggcattatga
     5041 gctggcgaag aaggaccccg cgtttcacgc cgaactcgac aacctcctca cccattacgt
     5101 cgggcgtccg agtccgctct atttcgccga gcggctgacg gactatctgc gccgcaaggc
     5161 agccgacgcg gagcatggcg cgaaaatcta tttcaagcgc gaggagttga atcacaccgg
     5221 cgcgcacaag atcaacaatg tgcttggcca gatcctgctc gcgcggcgca tgggcaagac
     5281 gcgcatcatc gccgagacgg gcgctggcca gcatggcgtc gcgacggcca cagcctgtgc
     5341 ccgcttcggc ctcgaatgta tcgtctacat gggggaggtc gacgtcgagc ggcagaagcc
     5401 gaatgtcttc cgcatgaata tgctcggcgc gaaagtggtg ccggtgcaat ccggcgcgcg
     5461 tacgttgaag gatgcgatga acgaggcgtt gcgggattgg gtcaccaatg tcgagaacac
     5521 gttctattgc attggcacgg cggccgggcc gcatccctat ccggcgatgg tgcgcgattt
     5581 tcagtgcgtc atcggcgaag agacgcgcac gcagatgcag gcggcagaag gtcggctgcc
     5641 ggattcgctc tttgcctgca tcggcggcgg ttcgaatgcg atcggcctgt ttcatccttt
     5701 cctcgatgat cggcagatcg aaatctatgg cgtcgaagct gccggctatg gcctcgacaa
     5761 ggcacatgcg gcgtcgctcg ccggcggccg gccgggcgtg ctgcatggca atcgtacgta
     5821 tctcttgatg gatgccgacg ggcagatcga agagggccat tcgatctccg ccggtctcga
     5881 ttatccgggc atcgggccag aacactcctg gctcaaggag acgggccgcg tcacctatct
     5941 ctcggcgacc gattccgagg cgctcgccgc gttccaactt tgcgcgcagc aggaaggcat
     6001 cattccggcg ctcgaacccg cgcatgcgct cgcgagagtg atcgacatcg cgccgcaaaa
     6061 gccgcgcgat catcttatgg tggtcaacat ctccggccgc ggcgacaagg acatcttcac
     6121 cgtcgccgat cacctcggcg gaatgtaatc gcttcactgt tctcgggagt tcaaaggtgt
     6181 cgtcgcaacc gatcatgtcc gatatcgaag gtttgaccaa agtcgcgccc ctgtcgcggc
     6241 gcggcttcat gacggcggcc ggtgctgccg ccgcgggcta tacgctcgcg gccgggccgg
     6301 tgcgtgccga tgcgattcac acttcggccg aagggctgga caccggcgat gcgaaagtcg
     6361 cggtgtctgg cggtgacatg ccgctgtatt tcgccaagcc cgcgggcgcg aaaaagccgc
     6421 cgatcatcct cgtcgcgatg gaggtcttcg ggttgcatga gtacatcaag gatgtcacgc
     6481 gacgcctcgg caagctcgga acgctcgcgg tcgcgccgga ttattacttc cgcctcggcg
     6541 atgtctcgaa aatctccgag cttccgaagc tcctgccgat cgtcaacagc aagccggata
     6601 aggaattgtt cgccgatctc gatgcgacgc ttgcctgggc catgaagcag ggcggcgatc
     6661 cggcgcgcgt gggcatcatc ggcttctgcc gcggcggccg caccgtctgg ctctattcga
     6721 cgcataatcc gcatctgaag gctggtgttg ccttctatgg cagccttatg gacccgccga
     6781 gcgccgccat gccgaagaat gcctatcagc tcgcgggcga ggtcaaggag ccggtgctcg
     6841 ggctttatgg cgcgcaggat cagggcatca cgccggatca ggtcgagaag atgaaggctg
     6901 cgcttgcggc ggatcacaag gcggcagaat tccacatcta tccgggcgct ccgcatggct
     6961 tccacgccga ctaccgcccg agctatcgca aagatgcggc agaagaaggc tggaagcgga
     7021 tgaaggcctg gttcgagaaa tacggcgttc tgccgtaaat cgttccgtcg gatttcatcg
     7081 ttccggtatt cgtgttagcc acccccgcca tgacccgtat cgacacccgt tttgccgaga
     7141 c
//
LOCUS       DASWOG010000351         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_44352,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000351 DASWOG010000000
VERSION     DASWOG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44352"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGB93_07395"
     CDS             <1..673
                     /locus_tag="VGB93_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0145961.1"
                     /translation="PRSGDDVVSLKNVHKSYGALSIYEGLNFAIRRRERWCVMGVNGA
                     GKSTLLKLVTGFTQPDEGTVSLGGTVKMGYFAQHAMDLLDGDHTVFQALEDAFPQAGR
                     GSLLALAGCFGLSGNEVEKKCRVLSGGEKARLVMAKMLFDPPNFLVLDEPTNHLDMAT
                     KEMLIAALSQYEGTMLFVSHDRHFLAALSNRVLELTPDGVHNYGGGYAEYVARTGHEA
                     PGLRS"
     gene            758..1960
                     /locus_tag="VGB93_07400"
     CDS             758..1960
                     /locus_tag="VGB93_07400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0145962.1"
                     /translation="MIRRFAVSRLLSVLVLIGGIVYLEVARPFGAFRLGAFVLLALLV
                     AVLTSFARGLWRDGLLIVTSLLFGICAIEAFATIVSPAWDIRTTQGFTVPVPVIGWGP
                     QHSGVFHAARFDPRTKRVIYDANYSINSELIRPTRSCKVGPTVAFFGDSFTFGQGVND
                     SETVPQYVSDAFGDNLRVLNLSFPGYGPQQFLRELEIGYFDRLVGPQPKLFVFMTTPW
                     HVIRSSCKASWVMDGPHYGLAGDKVAYQGVCDTGLARGLKQWAQHSATYRLFIEPRLE
                     KIDHADVDLFIRMTLAATDLAKTKYGVPTLIPFIRVPEGYLRPTGFSNNDIIRRLRDG
                     GADVIDVSLDKQQAAGEPVIIQGDDHATGLGNHLRATWLKDYIAQHYAAALSLASEGT
                     APICSGAK"
     gene            complement(1964..>2524)
                     /locus_tag="VGB93_07405"
     CDS             complement(1964..>2524)
                     /locus_tag="VGB93_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0145963.1"
                     /translation="ALGGRSILGDPRSPTMQKALNLKVKYRESFRPFAPSVLREHVSE
                     WFDLDSDSPYMLLVANVKETRCRKMSAEEEHLFGIDKLNILRSEIPAVTHVDYSARIQ
                     TVHKETNPRYHALIEKFAELTGCPVLINTSFNVRGEPIVLTPEDAFACFMGTEIEFLA
                     VGNCILRKDAQNPALKRDYKDAFEMD"
BASE COUNT          475 a          739 c          738 g          572 t
ORIGIN      
        1 gccgcgctcc ggcgatgacg tcgtgagtct gaagaatgtg cataaaagct acggcgccct
       61 gagcatctat gagggcctga acttcgcgat ccggcgcagg gaacgctggt gtgtcatggg
      121 cgtcaacggt gctggaaagt ccacgctcct gaagctggtg accggtttca cgcaaccgga
      181 tgaaggcacg gtctccctcg gcggcacggt caagatgggt tatttcgcgc agcacgcgat
      241 ggatttgcta gacggcgacc acacggtttt tcaggcgctg gaagatgcct tcccacaggc
      301 ggggcgtggc tctctactcg cgcttgccgg ttgtttcgga ttatccggta acgaggtgga
      361 gaaaaagtgc cgggtgctgt cgggcggaga gaaagcccgg ctcgtcatgg cgaaaatgct
      421 tttcgacccg cccaattttc ttgtgctgga cgagccgaca aaccatctcg atatggcgac
      481 gaaggaaatg ctgattgcag cgctgtcgca atatgagggg acgatgctct ttgtctcgca
      541 tgaccggcac tttctggccg cgctgtccaa tcgggtgctg gaactgacac ccgatggcgt
      601 tcacaattac ggtggtggct atgcggaata cgtcgcccgc acgggccatg aggctccggg
      661 acttcgcagt taggagtcct agcctcttcg tcgccccaat cttcctttta atgacgccat
      721 gcccacgcgc ccgcaggcgc cccaaaaaca tggtcgcatg atccgacgct tcgcagtttc
      781 ccgccttctc tccgttctcg ttctgattgg tggcatcgtc tatctggagg ttgcgcggcc
      841 gttcggtgcc tttcgtctcg gcgcattcgt ccttctcgcg ctgttggtgg ccgttctcac
      901 ctcgtttgcg cgcggcctct ggcgcgacgg cctcttgatc gtcacatcgc tcctcttcgg
      961 catctgcgcc atcgaggctt tcgcgacaat cgtctcaccc gcctgggata ttcgaacgac
     1021 ccagggcttt accgtgccgg tgccggtgat cggctggggg ccacaacatt ccggagtatt
     1081 tcacgccgcg cggttcgatc cacgcaccaa gcgtgtgatc tatgacgcga actattctat
     1141 caattccgag cttattcggc cgacacgctc gtgcaaggtt ggcccaacgg tcgccttctt
     1201 cggcgactca ttcacattcg gccagggcgt gaacgatagc gagacggtgc cgcaatatgt
     1261 gtccgatgcg ttcggcgata atttgcgcgt gctcaatctt agtttccctg gctatgggcc
     1321 gcagcagttt ctgcgggagc tggaaatcgg atatttcgat cgcctcgtcg gtccgcagcc
     1381 gaaactgttc gtttttatga caacgccatg gcatgtcatc cgcagctctt gcaaagcctc
     1441 ctgggtgatg gacggtccac actatggtct tgccggtgat aaggttgcct accaaggcgt
     1501 ctgcgatacg ggcctcgcgc gcgggctgaa acaatgggcg cagcacagcg ccacttatcg
     1561 cctcttcatc gagccgcgtc ttgaaaagat cgatcatgcg gatgtcgatc tgttcatccg
     1621 catgacactg gcggccaccg atctcgccaa gacgaaatat ggcgtaccga cgttgatccc
     1681 attcatccga gtaccagaag gctatctgcg gccgacgggc tttagcaaca atgacatcat
     1741 ccggcgttta cgcgatggcg gtgcagacgt catcgatgtc tcgctcgata aacagcaagc
     1801 cgcaggtgag ccggtcatca tccagggtga cgatcacgcg accggcctcg gcaatcatct
     1861 gcgcgcgacc tggcttaagg attatatcgc gcagcattat gccgcggctc tgtcgctcgc
     1921 cagcgagggc accgcgccta tctgctcggg cgcgaaataa tcctcagtcc atttcaaaag
     1981 cgtctttata atcgcgcttg agcgccgggt tctgcgcgtc cttgcgcaga atgcaattgc
     2041 cgacggcgag gaactcgatt tccgttccca tgaaacaggc gaacgcatcc tccggcgtga
     2101 ggacgatcgg ctcgccgcgc acattgaagc tcgtgttgat gagcaccgga cagccggtca
     2161 gttcggcgaa tttctcgatc aaggcatggt agcgcggatt ggtttctttg tgcaccgttt
     2221 ggatgcgcgc cgaataatcg acatgcgtca cggccggaat ctccgaacgc agaatattca
     2281 gcttgtcgat gccgaacaga tgttcttctt cggcggacat tttgcggcag cgcgtctctt
     2341 tgacgttggc gacgagcagc atatagggcg aatcgctgtc gagatcgaac cattcgctga
     2401 catgttcccg cagcaccgag ggcgcgaacg ggcggaaact ctcgcgatat tttactttga
     2461 ggttcagcgc cttctgcatc gtcggcgagc gaggatcgcc aaggatcgag cgtccgccga
     2521 gcgc
//
LOCUS       DASWOG010000352         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_228423,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000352 DASWOG010000000
VERSION     DASWOG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228423"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGB93_07410"
     CDS             complement(<1..874)
                     /locus_tag="VGB93_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0145964.1"
                     /translation="MAIGATLINAPDRVAAETASGLTIGSHLISYALDDIRYSDDPDL
                     ALNRLLEELSHVRHIRVGYRPSPGSRITDPITPSLAKSAPNWFVDFFVPRRVSESFPV
                     VYQGTRRGEIVMTAEPADEVGEVWDDLVFLISLLSAISVGIVVLTWLAVNYTLRPLRG
                     LVDGLNRLQRGQFDEVGEIRVAELQRVGDQFNRLAKSLARTESDNRLLIDRLMSIQES
                     ERKELARELHDEFGASLFGIRASASCVVQAGMAEGPIGPRFKEIVERAEAISSLAATI
                     QKNTNRLQERRQPIV"
     gene            1278..1847
                     /locus_tag="VGB93_07415"
     CDS             1278..1847
                     /locus_tag="VGB93_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0145965.1"
                     /translation="MKMRIGGGRANAWLFGGLSFCAGLVLAQSHGAFAHGAAPKKIEE
                     KIDIAAKPDVVWGIIKDFAKISTWNPAVVSSIAASDPDQGQERILTLKSGGKITDAQT
                     DYEAKTMTYSYRRVDDDVKVFPVSFYSATITVTPTATGSEVDWIGRFYRGDTSNEPPP
                     GLDDPDGIKAMTDFFDTGLKNLKMLAEKK"
     gene            1844..>2582
                     /locus_tag="VGB93_07420"
     CDS             1844..>2582
                     /locus_tag="VGB93_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0145966.1"
                     /translation="MIAWRVLTLGLTLAAVSPLAAAERVFATSQGGNKITEIAGGGTL
                     DFTSLPGPAAIAATPDGQRLFLTHPDHGTISVVDISSRKTIAHFTYPGVPFGIAFDAA
                     ENRLLVTDWNRDLLSSLDPVSGKVLREVPTGHSPAHIVLDSDHRRIFVCEREGNDIGV
                     YDSRTLAAIKHIQVGTAPYAIAYAPTTKRVYVANVRSNNVSVINAESLNVIGSIHVGP
                     MPYGVALTPDGKRLLVTIQAKNTLLMLD"
BASE COUNT          607 a          737 c          713 g          525 t
ORIGIN      
        1 agactattgg ttggcggcgc tcctgtaacc gattagtatt cttttgaatg gtggctgcga
       61 gcgacgatat cgcttcggcg cgttcaacaa tttccttgaa ccgtgggcca atcggaccct
      121 cagccatccc agcctggacg acgcatgaag ccgaggcacg aatgccgaac aatgacgcgc
      181 cgaattcatc atgcagctcg cgtgcgagct ctttgcgctc ggactcctga atggacatca
      241 accgatcgat aagtagacga ttatccgact cggtgcgtgc gagcgattta gccagccggt
      301 taaattggtc gccaacccgt tgcaattccg cgacgcggat ttcgccgacc tcatcgaact
      361 gtccccgctg caatcggttc agcccgtcga ccaatccacg caatggacgc aacgtgtagt
      421 ttacggcgag ccaggtgagg acgacaatgc cgaccgatat cgccgagagg aggctgatga
      481 gaaagacgag atcatcccag acttcgccca cttcgtctgc gggctccgcc gtcatgacga
      541 tctcaccacg ccgcgtacct tgataaacga ctggaaagga ttcgcttacc cgacgcggca
      601 cgaagaaatc cacgaaccaa ttcggcgcgc tcttggccaa agacggggta atcggatcgg
      661 tgattcgcga tccgggcgat ggacgataac cgacacggat atgacgaacg tgggaaagct
      721 cttcaagcag acggttcagc gccagatcgg gatcgtctga atagcggatg tcatcgagcg
      781 cataggaaat gagatggctg ccgatcgtca aaccggaggc tgtctccgcg gcgacgcgat
      841 ccggcgcatt gatcagcgtc gcgccaattg ccatgacaat tccgagcaac agaaaaacgg
      901 gcggaataag tataagccgc gtacgcagcg aagctgaagc ttcggtaaac tgaaacatca
      961 tcaacttgca atcatggtat gcgcgcctgg tccatctggc gcctgcaaca acatacattt
     1021 agctcatgat gctcgcttcg acgctgtctt ctttcggcaa aatgacatcc cactttttct
     1081 catgggacac gagacgaaga gaattttata agagaaaata tcccgacgcc gcgggataag
     1141 tctaatgacc cgtaagtata tatcatgcgt agtttggaac tttcgtagta taccctcgca
     1201 taacgaaata attcattgcg catctcttcg ttcgcggagc atagaagcat cgctctttag
     1261 aacgctgcgg agagatcatg aaaatgcgaa ttggtggtgg gcgtgcgaac gcctggctgt
     1321 ttggaggatt gagcttttgc gcggggttgg tcctcgcgca gtcgcacggc gcgtttgctc
     1381 acggcgcagc gcctaaaaag atcgaagaaa agattgatat cgccgccaag ccggatgtgg
     1441 tgtggggcat catcaaggac ttcgcaaaga tctcgacgtg gaatcccgcc gtcgtcagca
     1501 gcatcgcggc ttccgacccg gatcaaggcc aggagcggat tctgacgttg aaaagcggcg
     1561 gcaagatcac cgacgcgcaa acagattatg aagctaaaac gatgacctat tcctatcgcc
     1621 gcgtcgatga cgatgtgaag gtcttcccgg tgagcttcta ttccgcgacg atcaccgtca
     1681 cgcccacggc gacgggttca gaagtcgatt ggatcggccg cttctatcgt ggcgatacga
     1741 gcaatgagcc gccgcccggt cttgacgatc cggatgggat caaggcgatg actgatttct
     1801 tcgataccgg cctgaaaaat ctcaagatgc ttgccgaaaa gaagtgatcg cctggcgcgt
     1861 tttaacgctt ggcctgacgc ttgcggcagt cagccctttg gctgccgcgg agcgcgtctt
     1921 cgcgacgagc cagggcggca acaaaatcac cgaaattgca ggcggcggga cgctggactt
     1981 cacgagcctg cccggacccg ccgcgatcgc cgccacgccg gacgggcagc gattgtttct
     2041 gactcacccc gaccacggca cgatcagcgt cgtggatata tcaagccgaa agacgatcgc
     2101 acatttcacc taccccggcg tgccattcgg tatcgccttt gatgcagctg aaaatcgtct
     2161 gctggttacc gactggaacc gcgatctcct ctcgagcctc gatccggtaa gcggaaaagt
     2221 tcttcgcgaa gtccccaccg ggcactcacc ggcgcacatc gtcctggact ccgatcatcg
     2281 tagaatcttc gtttgcgagc gtgaaggcaa cgacatcggc gtctatgatt cccggacgct
     2341 ggcggcaatc aaacatattc aggtcggtac agcgccctac gccatcgctt acgcgcctac
     2401 gacgaagcgc gtctatgtcg ccaatgtccg cagcaacaat gtcagcgtga tcaacgcgga
     2461 gagcctgaac gtcatcggct cgattcatgt cggcccgatg ccctacggcg tggctctcac
     2521 gcccgatggc aaaagattgc tcgtcaccat ccaggcgaaa aacacgcttc tgatgctcga
     2581 ca
//
LOCUS       DASWOG010000353         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_265161,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000353 DASWOG010000000
VERSION     DASWOG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265161"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /gene="fabF"
                     /locus_tag="VGB93_07425"
     CDS             complement(<1..1203)
                     /gene="fabF"
                     /locus_tag="VGB93_07425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0145967.1"
                     /translation="MRRVVITGLGMVSPLANGVEASWERLIGAQSGARLIQGFETSDL
                     PCKIAMQVPRGDGSNATFNPDQWMDPKDQRKVDDYIIYGVAAATQALADAGWKPKTEE
                     ERERTGVLIGSGIGGLGGIAETAITLHEKGPRRVSPFFISGRLINLVSGYVSIMHGLK
                     GPNHAVVTACSTGAHAIGDAARMIALEDADVMVAGGAESAINRIGIAGFSACKALSTN
                     FNDRPKQASRPYDRDRDGFVMGDGAGCVVLESYEGAKARGAKIYAEVVGYGLSGDAYH
                     ITAPDPTGDGALRSMRGAVKRAGITAQDIDYINAHGTSTPLGDEAELTAVHKLLGNSQ
                     GMSSLSMSSTKSAIGHLLGAAGAVEAIFCTLAIRDQIVPPTLNLDNPSVTTSIDLVPH
                     KARKREVEF"
     gene            complement(1345..1587)
                     /locus_tag="VGB93_07430"
     CDS             complement(1345..1587)
                     /locus_tag="VGB93_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0145968.1"
                     /translation="MSDVAERVKKIVIEHLGVDADKVTDDANFIEDLGADSLDTVELV
                     MAFEEEFGVEIPDDAAESIITVGDAVRFLDKATAAS"
     gene            complement(1844..2581)
                     /gene="fabG"
                     /locus_tag="VGB93_07435"
     CDS             complement(1844..2581)
                     /gene="fabG"
                     /locus_tag="VGB93_07435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY0145969.1"
                     /translation="MFDLSGRMALVTGASGGLGSAIARALHHQGATVTLSGTRKEALQ
                     TLADELKERVYVVPCDLADPVAVEQLVPEAEAAMGGIDVLVNNAGLTRDGLMMRMKDA
                     DWDNVLAVNLTATFRLARAALKPMLKRRYGRIISITSVVGVTGNSGQTNYAASKAGMI
                     GLSKSLAREVAARGVTVNCVAPGFIESPMTDVLTDAQKSAILSTVPTGRLGSAAEIAG
                     AVVYLASSEAAYVTGETLHVNGGMAMI"
     gene            complement(2599..3549)
                     /gene="fabD"
                     /locus_tag="VGB93_07440"
     CDS             complement(2599..3549)
                     /gene="fabD"
                     /locus_tag="VGB93_07440"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0145970.1"
                     /translation="MAKAFVFPGQGSQAVGMGKALAEAYAPARRVFAEVDEALHQKLS
                     ALMFEGPEAELTLTANAQPALMATSLAVLRVLEREAGLDLGRDAAFVAGHSLGEYSAL
                     AAAGSFSIPDAARLLRRRGEAMQQAVPVGGGAMAALIGLDLDGATEVARAATEQTGAV
                     CSVANDNGGGQVVISGTKVAVDAAIEIAKSKGVRRAVPLAVSAPFHCPLMQSAADAMA
                     VALAEVEIGRPKVPLVANVLAAAIEEPEDIRQRLVAQVTGTVRWRESVVYMAAHGVDT
                     FIELGSGKVLTGLLKRIVADAHGVAVGTPDEIAAYPSSSV"
BASE COUNT          773 a         1225 c         1124 g          739 t
ORIGIN      
        1 gaattccacc tcgcgcttac gcgccttatg cggcacgagg tcgatcgagg tcgttaccga
       61 aggattgtcg aggttgaggg tcggcggcac gatttgatcg cggatcgcca aagtgcagaa
      121 aatcgcctcg accgcacccg ctgcgccgag cagatgtccg atcgccgatt tggtggacga
      181 catcgataaa gaactcatac cctggctatt gccgaggagc ttgtggacgg ccgtaagctc
      241 cgcctcgtcg cccagaggcg tcgaggtgcc gtgggcattg atataatcga tatcctgcgc
      301 cgtgatgccg gcgcgtttca cggccccgcg catcgagcgg agagcgccgt cgccggtcgg
      361 gtcaggcgcg gtgatgtgat aggcgtcgcc cgaaagaccg taaccgacaa cttcggcata
      421 gattttcgcg ccgcgcgcct ttgcgccctc ataagattcg agcacgacgc atccggcgcc
      481 atcccccatg acgaaaccgt cgcgatccct gtcgtagggc cgtgacgcct gcttcggccg
      541 gtcgttgaaa ttggtcgaga gcgccttgca ggcagaaaag ccggcgatgc cgatacggtt
      601 gatcgccgat tcggccccgc ccgccaccat cacatcggcg tcttcgagcg cgatcattcg
      661 cgccgcgtcg ccaatcgcgt gcgcgccggt cgaacaggcc gtaacgacgg catggttcgg
      721 ccctttcagg ccgtgcatga tcgagacata gccactgacg agattgatca ggcgacctga
      781 aataaagaac ggcgagacgc gccgcgggcc tttttcgtgc aacgtgatcg ccgtctccgc
      841 gatgccgccg aggccgccga tgccggagcc gatgagaaca ccggtccgct cgcgctcctc
      901 ttcggtcttg ggtttccagc ccgcatcggc caaagcctgg gtggccgcgg cgacaccata
      961 gatgatgtaa tcgtcgacct tgcgctggtc cttcggatcc atccattgat ctggattgaa
     1021 ggtcgcgttg ctgccatcgc cacgcggcac ctgcatggcg atcttgcagg gcagatcgga
     1081 ggtttcaaag ccctggatca ggcgggcacc gctctgtgcg ccgatcaggc gctcccacga
     1141 tgcctcaacg ccgttggcca gcggcgagac cattccaaga ccagttatca ccactctgcg
     1201 catcgcagat cgtccactcc tccgggcgtt ccgcccgcgt ggtttctatt tcaccgccgt
     1261 gccggattgg caaatcgcaa ccggcacagg gaaagttagg gaaaaaccgc cggtactatc
     1321 atatcgcgcg gcggggcgtt cagatcagct cgcagccgtt gccttgtcga ggaagcgaac
     1381 cgcgtcgccg accgtgataa tggactcagc cgcatcatcc gggatttcaa cgccgaattc
     1441 ttcttcgaaa gccatcacga gttcaacggt gtcgagcgag tcggccccca gatcttcaat
     1501 gaaattcgcg tcgtcggtga ccttgtcggc gtccacgccg agatgttcga tgacaatctt
     1561 tttcacgcgc tcggcaacat cgctcattcg tttaaccctt cgtccttaat ctttaagtgg
     1621 gtgccagccg ttatcaaaaa tacatcttaa atcaagcccc ccgaaggcag acgccctcat
     1681 agcttgatca cgtttctcga aaactgcggc agatttccta agcttttttc cggtcccacg
     1741 gaaagacgag aggaccgtcc gtaagcagat attcggttgg taacatactt cattcgtatt
     1801 ggcgagggcc ggaccttagc cggcgcaaat cggcataaca aactcaaatc attgccattc
     1861 cgccgtttac gtggagcgtt tcgccggtaa cgtaagcggc ctccgaactc gccagataaa
     1921 ccacggcgcc cgcaatttcc gctgccgagc caagccggcc agtcggcacc gtcgaaagaa
     1981 tggccgactt ctgcgcgtcg gtgaggacat cggtcatcgg gctttcgatg aaacccggcg
     2041 cgacgcaatt caccgtgaca cctcgcgcgg ccacttcgcg agcaagactt ttggacaggc
     2101 cgatcattcc ggctttcgaa gcggcatagt tcgtctgccc cgaattgccc gtgacgccga
     2161 cgaccgaggt aatactgata atgcggccgt aacggcgctt cagcatcggc ttcaaagccg
     2221 cgcgtgcgag ccggaacgtc gcggttaaat tcaccgccag cacgttgtcc caatcggcat
     2281 ctttcatacg catcatgagg ccgtcgcgtg tcaggccggc attgttgacg agcacgtcga
     2341 taccacccat cgccgcttcc gcttcgggca ccaattgctc gacggccact ggatcggcga
     2401 ggtcgcaggg cacgacataa acgcgctctt tcaattcgtc ggccaaagtc tggagcgcct
     2461 ctttgcgggt gccggagagc gtgacggtcg caccctgatg gtgcaaggcg cgcgcgatgg
     2521 cggagccaag accgccgctg gcaccggtga cgagggccat acggccggaa aggtcaaaca
     2581 tgagcggccc tcaaactttc atacggaaga agacggatac gcggcaatct cgtcgggcgt
     2641 gccgaccgcg acaccgtgcg cgtcggcgac aatccgtttc aaaaggccag tgagaacttt
     2701 gcccgagcca agctcgatga aagtatcgac gccatgcgcg gccatataga cgacgctctc
     2761 gcgccagcgc actgtgccgg tgacttgcgc cacaaggcgc tgccgaatgt cctccggctc
     2821 ctcgatggcc gccgcgagaa cattggcgac aagaggcact ttcggccgcc cgatctcaac
     2881 ctcggccagc gccacagcca ttgcgtcggc ggcagactgc atcaaaggac aatggaatgg
     2941 cgccgatacg gcaaggggaa cggcgcggcg cacgcccttg ctcttggcaa tttcgatcgc
     3001 cgcatcgacg gcgactttag tgccggaaat aacgacctgg ccgccgccgt tgtcattcgc
     3061 aacggagcaa accgcgccgg tttgctccgt tgctgctcgt gcaacttctg tagcgccatc
     3121 aagatcgagg ccgatcaagg ccgccatcgc gccgccgcca accggaacgg cctgctgcat
     3181 ggcctcgcca cgccgccgca gcaatcgcgc ggcatcaggg atcgaaaacg aaccggcggc
     3241 ggccagcgca gaatattcgc caagtgaatg gcccgcgaca aaggccgcgt cacggccaag
     3301 atcgagtccc gcttccctct ccagcacgcg caaaaccgcg aggcttgtcg ccatcagtgc
     3361 gggctgtgcg ttggcggtca acgtcagttc ggcctcgggg ccctcgaaca tcaacgccga
     3421 gagtttttga tgcagggctt catcaacttc cgcaaaaacc cgccgtgccg gcgcgtaggc
     3481 ttcggcgagc gccttgccca tgccgacggc ctgcgagccc tgccccggaa atacaaatgc
     3541 tttcgccata cgcgcgtttc cgctctatta ctttcgttgt tcccggcgct gccgactggc
     3601 attggtaaac ggacgtgtca agtttctggt gatggcgcgg cggccgtccg gatttcagtt
     3661 gcctccacga tgcggcgcgc catgatgccg aatctgcgat ctatcgtagg cggagcgaac
     3721 ctcaatatgg acattccagg caaagcctgc ggcacctgca gtatgtgctg taaaatactt
     3781 gagatcgaag aattgacgaa gccggccggc ccttattgcc cgaattgcaa gctcggcgac
     3841 ggctgcggca tctatcattc g
//
LOCUS       DASWOG010000354         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_337930,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000354 DASWOG010000000
VERSION     DASWOG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337930"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGB93_07445"
     CDS             <1..255
                     /locus_tag="VGB93_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0145971.1"
                     /translation="RPHIAQNTSGRRSTIDRRTTRHQAYSASQRIRKRIEEAFGWMKT
                     IAGQRKTKFRGVDRVGWAFAFAAAAYNLVRLPKLIEEIAP"
     gene            429..866
                     /locus_tag="VGB93_07450"
     CDS             429..866
                     /locus_tag="VGB93_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0145972.1"
                     /translation="MMQPAFSWQTWAGLSAVFAALTAILAKLGVGGINSNFATLIRTV
                     FILILAAIIVSVTRAWQAPSSISPRSFLFLALSALATGASWLCYFRALQLGQASKVAP
                     VDKLSVVLVALFGVTFLGEKLSAMNWLGVIFVGAGAILLAFKF"
     gene            898..>1690
                     /locus_tag="VGB93_07455"
     CDS             898..>1690
                     /locus_tag="VGB93_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0145973.1"
                     /translation="MTEEIFIGVLIIFAIILVLPIFRSIFTIQQAHAGIIERFGKFSR
                     VVEPGLHVKLPYIDRLAALLSLKIEQNIITADTKTKDNVFVKVNIAVNYRVVAGRERE
                     AFYVLSDAKGQIQAYVLDVVRSKIPAMTLDEVFEKKDDVAQAVTQHLSELMQQYGFEI
                     VSSLVVDVQPDPTVVAAMNEIQAQTRLQLAAQAKGEANKILAVKAAEAESESKALQGK
                     GIADQRKAIIAGLNDSVTSLSTTAHTPPAEVLRTLLMTKYFDTTRE"
BASE COUNT          354 a          489 c          482 g          365 t
ORIGIN      
        1 cgtccgcaca tcgcgcaaaa taccagcggg cggcgttcaa ccatcgaccg gcgaacgacg
       61 cgccatcagg cctattcggc gagccagcgt atccgcaaac ggatcgagga ggcctttggt
      121 tggatgaaga ccatcgccgg gcagcggaag acaaagtttc gcggcgtcga tcgcgttggc
      181 tgggctttcg ccttcgccgc ggcagcctac aacctggtca ggctgccgaa actgatcgag
      241 gagatagcac cataatgcca cctaaggcgt ccgctcatca aagccacatc ctccgaccat
      301 tatcatcgac aaagccagtc tcggaaaatc agcaacaact ctgtccgcgc ccgaagcgcc
      361 gcaatttttc agcagcctgc tagtcccgcc gccaaagcag cgcgccggca aacagcgcgc
      421 gtagaggcat gatgcaaccg gcattctcct ggcagacttg ggccggcctt tctgcagttt
      481 ttgccgcttt gacggcgatt ttggcgaaac tcggcgtcgg gggcatcaat tcaaatttcg
      541 caacgctgat ccggactgtc tttatcctca tcctcgccgc gatcatcgtt tccgtcacgc
      601 gggcatggca ggcgccgagt tcgatttcac cgcgaagctt cctgtttctg gcgttgtcgg
      661 ctttggcgac gggcgcctcg tggctctgct attttcgcgc gcttcaattg ggccaggctt
      721 ccaaagtcgc gccggtcgat aaactgagtg tcgtcctcgt cgcgcttttt ggtgtcacat
      781 ttctcggcga aaagctttcc gccatgaatt ggcttggggt catattcgtg ggcgccgggg
      841 cgatcttgtt ggcttttaaa ttttgatacg gccggcgcgg atcggcgagt gggaaacatg
      901 accgaggaaa tttttatcgg cgtcctgatt atcttcgcca taattttggt gctgccgatc
      961 ttccggtcga tcttcaccat tcagcaggcg catgccggca tcattgagcg gttcggcaaa
     1021 ttcagtcgcg ttgtcgaacc cggcctgcac gtcaaacttc cctatatcga caggctcgcc
     1081 gcattgctgt cgttgaagat cgaacagaac atcatcaccg ccgacacgaa gacgaaggac
     1141 aatgtcttcg tgaaggtcaa tattgccgtc aattatcgcg tggtcgctgg ccgtgagcgt
     1201 gaggcgtttt atgtgctgtc ggacgccaaa ggacagatcc aggcttatgt cctcgatgtc
     1261 gtccgttcga aaattccggc gatgacactc gacgaagttt tcgagaaaaa ggacgatgtc
     1321 gcccaggcgg tcacgcagca tctttcggaa ttgatgcagc aatacgggtt tgagatcgtc
     1381 agttcgctcg tcgtcgatgt gcagccggac cccacggtcg tcgccgcgat gaacgaaatc
     1441 caggcgcaaa cgcggcttca gctcgcggcg caagccaaag gcgaggccaa taagattttg
     1501 gcggtgaagg ccgccgaggc cgagtccgag tcgaaggctt tgcaaggcaa gggcattgcc
     1561 gaccagcgca aggccattat cgcggggctc aacgattccg tgacaagcct ctcgacaacg
     1621 gcgcacacgc cgccagcaga agtgctgcgc acgctgctga tgacgaaata tttcgacacg
     1681 actcgggaaa
//
LOCUS       DASWOG010000355         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_136770,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000355 DASWOG010000000
VERSION     DASWOG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136770"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..735
                     /locus_tag="VGB93_07460"
     CDS             286..735
                     /locus_tag="VGB93_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0145974.1"
                     /translation="MLTKKGKYGLKAMVHLAGYPPGEPSLVNDIATINDIPKKFLDTI
                     LGELRNAGFVNSKKGKGGGYTLARSAQDIRVGHIIRVLDGPLAPIQCASKTAYRRCDD
                     CIDETTCGVRLLMLQAREAITQVLDNRTLADMRALALSGEQEFTYTI"
     gene            853..2133
                     /locus_tag="VGB93_07465"
     CDS             853..2133
                     /locus_tag="VGB93_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY0145975.1"
                     /translation="MSVEPKVLLRSLFDAAVSAADPARVLAQYLPPPPKGQTIVIGAG
                     KGAASMARAVEAAWTGELSGVVVTRYGHHLPTRDIEVLEASHPVPDAAGLTGSKRLLA
                     AVQGLTADDLVICLISGGASALLPLPLEGLDLADEQTLNKALLKCGATISEMNCVRRH
                     LSAIKGGRLAVACHPAKLVTLLISDVPGDEPVDICSGPTIADPTTCQDALAILIRYGI
                     EMNDKVRDVLASGRGESIKPGDPRLKNSKIHMIATPQMALEAAADVGRKHGYTVHILS
                     DDIEGEARDVGKVMAAISRQIARHNQPFTVPCLILSGGETTVTVRGNGRGGRNVEFLL
                     SLGVALAGHPGIYALAGDTDGVDGLEDIAGAYLGPDTLARGWKLGIKPPDALANNDGH
                     SFFEALGDSVVTGPTLTNVNDFRAILITPEAAEA"
     gene            complement(2137..2448)
                     /locus_tag="VGB93_07470"
     CDS             complement(2137..2448)
                     /locus_tag="VGB93_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0145976.1"
                     /translation="MAEWADDHDQAASLSLDPVLDIKAVSLLAENLLAHRGSDIIVDA
                     GQVERLGAQSLQVLLSAVATWRADGHLLEVANPSEAFTEGLRIFGVDAESWLQHPHAV
                     G"
BASE COUNT          503 a          814 c          766 g          483 t
ORIGIN      
        1 gcccgagtcg acaaggcttt aatgacaccc gaggctttct cggcgacgcc cggtcaggcc
       61 gcgcaactgg cgcgccagcg cagcgatctt gcgcgcgcgc ttgccgctgc ggaagatgaa
      121 tggctgcagc tttccgccga agtcgaactc gcggcccggt gaaacttggg cgcggccgaa
      181 aactcgtgct aggctggcat ttctcgattc tgatcataaa tttagtggac aatgcgttaa
      241 gcgctacaat atgcgggtga cgcaaatcct tcgcgaattc cgaacatgtt gacgaaaaaa
      301 gggaaatacg gcctcaaggc gatggtgcat ctcgccggct atccgccggg cgagccatct
      361 ctcgtaaacg atattgcgac catcaacgac attccgaaga agtttctcga tacgatcctc
      421 ggcgagttgc gcaacgccgg cttcgtcaat tcgaaaaagg gcaagggcgg cggctatacg
      481 ctcgcacgct cagcacagga catccgcgtc ggccatatca tccgagtcct cgatgggccg
      541 ctggcgccga tccaatgtgc cagcaaaacc gcctatcgac gctgcgacga ctgcatcgac
      601 gagacgactt gcggcgtccg gctgctcatg ctgcaggcgc gcgaggcgat cacccaggtt
      661 ctggacaatc ggacgctggc cgatatgcgc gctctggcct tgagcggcga gcaggaattc
      721 acctatacga tctgacttct ggacgcggct tccatggccg aggatgatcg gctgaggcac
      781 ccgccgcggc cgtccgcatc tcatttggcc ataataagct tataaaacaa ataaaagggc
      841 ggttctctta agatgtcagt tgaaccaaag gttttgctcc ggtcgctgtt tgatgccgcc
      901 gtctccgccg ccgatccggc gcgtgttctg gctcaatatc ttccgccgcc gccaaagggc
      961 cagacgatcg tcatcggcgc tggcaaggga gcggcctcca tggcgcgagc cgtcgaggcg
     1021 gcgtggacgg gcgaattgtc gggcgtcgtc gtcacccgct acggccatca tctgccgacc
     1081 cgcgacatcg aggttttgga agcgagccat ccggtgcccg acgctgccgg gctcaccggc
     1141 tcgaagcgcc tgctcgccgc cgtccagggg ctgactgcgg acgatcttgt catctgccta
     1201 atttccgggg gcgcctcggc gctcctgccg ctgcccctcg aaggtctcga ccttgccgat
     1261 gagcagacgc tcaacaaggc gctgctcaaa tgcggcgcga cgatcagcga gatgaattgc
     1321 gtgcgccggc atctctccgc catcaagggc ggacggcttg ccgtcgcctg tcatccggcc
     1381 aagctcgtga ccctgctgat ctcggatgtt ccgggcgacg aacccgtcga tatctgctcc
     1441 ggcccgacga tcgccgaccc gaccacctgc caggacgcac tggcgatcct tatccgctac
     1501 ggcatcgaga tgaacgataa ggtgcgcgat gttctcgcca gcggacgtgg tgaaagtatc
     1561 aagcccggcg acccgcgttt gaaaaacagc aaaatccata tgatcgccac gccgcaaatg
     1621 gcgctcgaag ccgcggcaga cgtcggccgc aagcacggct acacggttca tatcctcagc
     1681 gacgatatcg aaggcgaggc gcgcgacgtt ggcaaggtga tggccgccat ttcccggcag
     1741 atcgcgcggc ataaccagcc tttcacagtc ccttgcctga tcctctccgg cggcgagacc
     1801 acggtgacgg tgcgcggcaa cggccgcggc ggccgcaatg tcgagtttct gctgtcgctt
     1861 ggcgtcgcgc tcgcgggcca tcccggcatt tatgccctgg cgggcgatac cgacggcgtt
     1921 gacgggcttg aagatatcgc cggcgcttat ctgggacctg acacacttgc ccgcgggtgg
     1981 aagctcggaa tcaagccgcc ggatgcgctg gcgaacaacg acgggcacag cttcttcgaa
     2041 gccttgggcg acagcgtcgt cacgggtcca acgttgacga acgtcaatga cttccgcgca
     2101 attctgatca cgccggaagc ggccgaagcc tgagactcag ccgaccgcgt gcgggtgttg
     2161 taaccagctt tcggcatcga caccgaaaat ccgcagcccc tcggtgaaag cctcggacgg
     2221 attcgcaact tcgagcaaat gtccgtcggc acgccacgtc gcgaccgccg acagcaaaac
     2281 ctggagcgac tgcgcgccga gccgctcgac ttgccccgca tcaacgatga tatcgctgcc
     2341 gcgatgggcc aaaagatttt cggccagcag cgagacagct ttgatgtcta aaacggggtc
     2401 taaggaaagg cttgctgcct ggtcatgatc gtcagcccat tccgccacgg cattgctcca
     2461 ttcttacgca tactcggcgc taatctggcg ggcggaagtt accagctgct tccgtttgcc
     2521 gtagcttctg gcacatgggc cgcgcttttt ttgcaaacag cctttc
//
LOCUS       DASWOG010000356         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_100251,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000356 DASWOG010000000
VERSION     DASWOG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100251"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VGB93_07475"
     CDS             complement(<1..498)
                     /locus_tag="VGB93_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0145977.1"
                     /translation="MAKSVDINLALQGGGAHGAFTWGVLDRLLEDETLQFGWISATSA
                     GAVNAVALAAGLAEGSRAKAREKMYKIWHAIHQAGVPDLTRLNPFLNGLVRPSQLTTR
                     LATMLSPYEFNPLGFDPLRRLITDHVDFDLIRSKSPVDLLIAATHVATGRARLFRRDE
                     ITIDAV"
     gene            complement(835..>1622)
                     /locus_tag="VGB93_07480"
     CDS             complement(835..>1622)
                     /locus_tag="VGB93_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0145978.1"
                     /translation="RRRVHRAEIADKLIADAVALRADHIAITGDLINLGLPTEYEAAL
                     AWLKGIGSPRDVTVIPGNHDIYSSLYGDPGVARWAEYMGGENDTLAFPFVRHVGPIAL
                     VGLNSAVETPPFVASGQLGQHQLEIAAEQLAALGEQDIIRVVLIHHPPLPSLAPPRRG
                     LRDAAHLAHLLERGDAELVLYGHNHKTRVDWLPSRKKAVPLVGVASASASVAHGEEPH
                     ASNNLFTFFKSENGLRIRHTVRGIDAPETPVKKISETILTATT"
BASE COUNT          345 a          485 c          460 g          332 t
ORIGIN      
        1 caccgcatca atggtgatct cgtcccgccg aaagagacgc gccctccctg tcgccacatg
       61 cgtggcggca atcaacaggt cgacgggcga cttagatcgg atgagatcga aatcgacgtg
      121 atcggtgatc agccggcgca atggatcgaa gccgagagga ttgaattcgt agggcgacag
      181 catcgtcgcg agacgtgtcg taagctgtga cggacgcaca agtccgttta gaaatggatt
      241 gagacgcgtc agatccggga cgcccgcctg atggatggcg tgccagatct tgtacatctt
      301 ctcgcgcgcc ttcgcccgcg agccctcggc caaaccggcg gcaagcgcga cggcgttgac
      361 ggcgcccgcg cttgttgcgc tgatccatcc gaactgaaga gtctcatctt ctagcaggcg
      421 atcgagaacg ccccacgtaa aggcgccatg agctccgccg ccctgaaggg ccagattgat
      481 gtcgacagat ttcgccaaaa ccgcattgct ccccgcaggc cactaagaca cgaaagccgg
      541 cgcgattcca tatcattacc tctcgcggca agcttatgtc atcggtgccg cggcagaacg
      601 actgaccctt tcagctagtt tattcactag cgtccaaata ttacacgcgc tcgtcacgca
      661 tctcttgctc aagccatgtg cgcacgtgac atctgacttg ctcagagcct aagcatggac
      721 tcatttccag cggtgagaaa tcgcgcgaat gcgctattgc cgcctcacaa ctgtgtaatt
      781 tcaaaacgcg tggcatttgg ccgcgacgca tgcagtcgat gaaatcgatc tcgctcacgt
      841 cgtcgcagtc agaattgttt cactgatttt tttgactggc gtttccggtg catcgattcc
      901 gcgcactgtg tgacggatac gaagtccatt ttcacttttg aagaacgtga aaagattgtt
      961 gctcgcgtgc ggttcttcgc cgtgcgcaac agacgcggac gccgacgcga cacccaccag
     1021 tggaacagcc ttcttgcgcg acggcagcca gtccacgcgg gttttgtgat tgtggccgta
     1081 aagcacaagc tccgcgtcgc cccgctcgag aagatgggcg agatgcgccg catcgcgcaa
     1141 cccgcggcgc ggtggagcaa gactggggag cggcggatga tggatcagca ccacgcggat
     1201 gatgtcttgt tcaccgagag ccgcgagttg ctcagcagca atctcgagct gatgctgtcc
     1261 aagctgaccg ctggcaacga acggtggcgt ctcaaccgcg gagttcaatc caacgagcgc
     1321 aatcggaccg acgtgccgca cgaaaggaaa ggctagcgta tcgttttccc ctcccatgta
     1381 ttccgcccat ctcgcaacac cggggtcgcc gtagaggctt gaataaatat cgtggtttcc
     1441 gggaatgacg gtcacgtctc tcggcgaacc tatgccttta agccaggcga gtgccgcctc
     1501 gtattccgtc ggcagaccaa gattgatgag gtctcccgta atcgctatat ggtcggcgcg
     1561 aagtgccacc gcatccgcaa tcagtttatc ggctatctcc gcccggtgga cgcgccgccg
     1621 ct
//
LOCUS       DASWOG010000357         5826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_136815,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000357 DASWOG010000000
VERSION     DASWOG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136815"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..690)
                     /locus_tag="VGB93_07485"
     CDS             complement(250..690)
                     /locus_tag="VGB93_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145979.1"
                     /translation="MHLSHRVRASLVGLLALFALSSSTTAFADSGAVRLSIVKGGWFV
                     GANGGGGSLYFHGRRYALTVGGIDAGLVFGLSHTTLLGRVLHIQRPSDIAGVYGAAGA
                     GATVGVGARLIRLANDKGAVLELEGFQVGLMVNADLSGLVIGLR"
     gene            903..1178
                     /locus_tag="VGB93_07490"
     CDS             903..1178
                     /locus_tag="VGB93_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145980.1"
                     /translation="MKVYAIVCDFVARDAHPDFFGCLEALGEAWCHVNESIWLVKTSL
                     SPRQIKLRLSPHLNDCDQLLILFCTRGAWQGFNDISALGLKAVFQTQ"
     gene            complement(1188..2876)
                     /locus_tag="VGB93_07495"
     CDS             complement(1188..2876)
                     /locus_tag="VGB93_07495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0145981.1"
                     /translation="MFMSRRSGRTPPAKTPSLLAAAPVDVFIATYNEPYNILERTIVG
                     AKAIDHPDLRVWILDDGARDWVEKLAEDLGIHYAHRVKGAHAKAGNINNGLQQALSTG
                     RRPEFVLILDADFVPYRNILKRTLGLFAEPDIGIVQTPQHFFNPDPIQNNLAMTRVWP
                     DEQRFFFNTLLDSKDAWGAAFCCGTSAVFRVDALVAAGGMATETVTEDMLTSFKFSEH
                     GFRTIFLNEMLSMGLAPEGLSSYISQRSRWCLGAIQQMYTRWSFAGPAKIGIINRISS
                     FDGALYWISNAPFKLLMITAPLVYWWTQTAVISSTAAGMLCYLAPYVVCSTFFMAFYA
                     RKRIIPIMTDVTQLLMSFSMVATVAIALVKPWGHPFKVTAKGISADRVVIQWRYLLPF
                     AAIALTTMLGIARNTAQFSPVYGTDGYALNVFWSIFNIAILVIACAVCVEVPRRRVEE
                     RFNSGEHATMRLANGTQVPCVLRDISLGGAHLTIDFGRLRADDQGSLLLSNGIEAPFT
                     QVRQSQGGVAVKFNLDRQTRRHLIALLFTGGYANEVPQVRLRHLFATLFKRLAA"
     gene            complement(3143..4402)
                     /locus_tag="VGB93_07500"
     CDS             complement(3143..4402)
                     /locus_tag="VGB93_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0145982.1"
                     /translation="MSEFSARASVQPAPNAVGLTFHLPWLRLAKLGATVTVLTVGGYA
                     ILSNEFAVVTDNAVVSSYTVPLRTPIDGVVYAMPLKVGDPVHQDDRIATVTDDRVNNL
                     HLVDLREHLTRARAELAVVSAEMNSLETLRAALQLRADYFKEWSTARIQGSLTEAQDQ
                     LLALNYRRDEAKQTLDRRAVLAERGFSSTADLDKAKADFGTAFNEAEAQRGRIISFEA
                     QLAAIKSGIITDPGSTDTPYSQQREDEITIRLQDLTQQRQFIVSDVSETAGRLASEEA
                     RIVKMRTAIMVAPTAGMIWKINAAQGERINAGEPIAEIVDCNSAFIIADVPQARVPDI
                     SAESKVEFMISGETAKRYGHVASVTSDPTDGDHNLAAIPFEEKGATATVRIRIDGEKG
                     CLVGRRARVVLPSNGSDFASRIRNYLP"
     gene            complement(4835..5452)
                     /locus_tag="VGB93_07505"
     CDS             complement(4835..5452)
                     /locus_tag="VGB93_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0145983.1"
                     /translation="MKILIVDDHLAVREGVRCLFAAMPGTEIFDAASAQEALSVYRSK
                     APDVLLMDLNLPNSSGLELLSNVLIEDRLAKIVVFSVHVEPIYVSRTFIAGALGYVSK
                     GASTKEIIAAVQAVAAGGRYIEREIATQMVLSPSGGANPLEKLTAREIDIARLLAEGK
                     TLGAIALVLGVSHKTVANACSIIKSKLGAERTFDLIRLLYQMEDR"
     gene            complement(5556..>5826)
                     /locus_tag="VGB93_07510"
     CDS             complement(5556..>5826)
                     /locus_tag="VGB93_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0145984.1"
                     /translation="RAVDRDTGEILWQRKLGSGIIGNPITYEVKGKQYVSVLSGIGGW
                     IGLPVTAGLDLNDKFGAIGATAMTKAAGLALIPQGGMLSTFRVYQ"
BASE COUNT         1317 a         1659 c         1669 g         1181 t
ORIGIN      
        1 ttcgcgaact tttggatcat cgccgtacgg ccagcctgca agatactccg tcaactgagc
       61 cgtaatcgcc tcaatcaagc gaccgcggcc aatagaagtt attctctagg agagaggcgt
      121 ccgcctagcc gccgctatct accggtcggg gctgctttgt caaagttata atccccaagg
      181 gggggagcac ttcgtgcatt taaccaaaga agacggctgc actacccgca acctcttccc
      241 gatggccatc taccttagcc cgatgacgag cccgctcagg tcggcgttca ccatcaggcc
      301 gacttgaaag ccttccaatt ccaagacagc gcccttgtcg ttggcgagcc ggatgaggcg
      361 cgcgccgact ccaaccgtgg cacccgcacc ggccgcgcca tagacgcccg cgatgtccga
      421 tggccgttgg atgtgaagca cccgtccaag cagagtcgta tgcgacaggc cgaagacaag
      481 cccggcatcg atgccgccga ccgtcagcgc atatcgccgc ccgtggaaat acaggctgcc
      541 gccgccgcca ttcgcaccaa cgaaccagcc gcccttcacg atggacaggc gcacggcgcc
      601 gctgtcggcg aaggcggtgg ttgacgaact cagagcaaaa agggccagca gaccgacaag
      661 gcttgctcga acccgatgag acaaatgcat aatgttcccc ccgaaacccg atcgcgcaat
      721 cccaccagcc tatatcaggg cggcaaaaag aaaaccaatt cccgggagcc agctcgaaat
      781 taggtggtga gatgggcgtc tttctgcact cggcgcaact cttcgttcat ctcaattttt
      841 aagagcgggg caaccaatat cactcatgct gccgagatta tttaaaaaac ggtgattgcg
      901 aaatgaaagt ttatgccatt gtttgcgact ttgtcgcgag ggatgcacat cccgattttt
      961 ttggatgtct tgaggctctg ggcgaagctt ggtgccacgt caatgaatcg atctggctcg
     1021 tcaagacgtc actgtcgcct cgacaaatca agttgcgcct gtcgcctcat ctgaacgatt
     1081 gcgatcaact gctcatactc ttttgtacgc ggggcgcctg gcaaggcttt aacgacattt
     1141 ctgcgctcgg gcttaaggcc gttttccaaa cgcaataagt cgcctgatca ggcggccagt
     1201 cgcttgaaca gcgtcgcgaa gaggtgcctg agtctgactt ggggcacttc attggcatag
     1261 ccgccggtga acagcagggc gatgagatga cgccgtgttt gccgatcaag gttgaatttc
     1321 actgcaacac caccctggga ctggcggacc tgggtaaacg gcgcctcgat tccgttggaa
     1381 agcaggagcg atccctggtc gtctgcgcgc agacgtccaa agtcgatcgt gagatgagcc
     1441 cctccgagag aaatatcacg caggacacag ggaacttgcg tcccgttcgc gaggcgcatc
     1501 gtcgcgtgct ctccggaatt gaaacgctcc tcgacacgcc gccgcgggac ctcaacgcag
     1561 actgcgcagg cgatgacgag gatcgcaata ttgaaaatgc tccagaagac attcagggca
     1621 tatccgtcgg ttccgtagac gggagaaaat tgggccgtgt tccgcgcgat cccgagcatc
     1681 gttgtcaacg cgatcgccgc gaagggaagc aaatagcgcc attggatgac gacccgatct
     1741 gctgaaattc ccttggccgt gactttgaac ggatgacccc acggtttaac cagcgcgatt
     1801 gcgacggtcg cgaccatcga gaagctcatc agtaattgcg tcacgtccgt catgatcggg
     1861 ataattcgct tgcgggcgta aaaggccatg aaaaaggtcg agcatacgac atagggcgca
     1921 aggtaacaga gcatccccgc agccgtgctg gaaatgacgg ccgtctgcgt ccaccagtag
     1981 acaagcggcg cggtgatcat cagtaatttg aagggcgcat tgctgatcca gtaaagcgcg
     2041 ccgtcgaagg acgaaatccg gttgatgata ccgattttcg ccggccccgc gaatgaccag
     2101 cgcgtgtaca tctgctgaat cgcacccagg caccagcgcg aacgctgtga aatgtagctg
     2161 ctgagaccct cgggcgcgag ccccatgctg agcatttcat tgagaaatat cgtgcgaaag
     2221 ccgtgttcgc taaatttgaa ggaggtgagc atatcctccg tcaccgtctc ggtggccatc
     2281 ccgccggcgg cgacgagcgc atcaacacga aacacggccg aagttccgca gcaaaatgcg
     2341 gcgccccatg catcttttga atcaagcaat gtgttgaaga agaaccgctg ctcgtcgggc
     2401 cagacgcgcg tcatcgccag attgttctgt atcggatccg gattgaaaaa atgctgcggc
     2461 gtctggacga ttccgatgtc aggctcggca aagagcccta gagttcgctt cagaatatta
     2521 cgataaggga cgaaatcagc atcgagaatc aggacaaact ccggtcgtcg gccggtcgat
     2581 agagcctgct gcaaaccgtt gttgatgttc ccggccttgg cgtgggcgcc cttcacgcgg
     2641 tgggcataat gaatgccaag gtcttccgcc aatttttcga cccagtcgcg tgcgccatca
     2701 tcgagaatcc agacgcgcag atccgggtga tcgatcgcct tcgcgccaac gatcgttcgc
     2761 tcgagaatgt tataaggctc gttataagtg gcgatgaaga catcaacagg tgccgcggca
     2821 agcaaggagg gcgtttttgc cgggggcgtc cggccactgc gccgagacat aaacatgatt
     2881 acgctcatag agctgcatat cgacatcgtc tcgaaagcga agaacagcca cgcccatgtc
     2941 tgctggaaaa aatcttgatt tacaggcatc gaatattcga ggcgccacca aacgtaacgc
     3001 gccgcaagcg agatacacag agccgagtat gctacgcgca cgacgcctgc gaggcgggtt
     3061 tcgaattgga tgaggagcgc agctacgccg acgctgagga aaagagggcc aaaagagccg
     3121 agatcaagag acatggtctt gatcagggca ggtagttgcg gatgcgtgaa gcgaaatccg
     3181 acccgttcga cggcaatacg acgcgcgccc ggcggccgac aagacatcct ttctcgccgt
     3241 cgatgcgaat acgcacggtc gctgtcgcgc ctttctcttc aaacgggatc gccgcgagat
     3301 tatgatcgcc atcagtcgga tcgctggtga cggatgcgac gtggccatat cgcttcgccg
     3361 tctccccgga gatcatgaat tcgactttgc tttcggccga gatgtcagga acacgagcct
     3421 gcggcacgtc ggcgatgatg aatgccgagt tacaatccac aatctcggca attggctcgc
     3481 cggcgttgat acgctcgccc tgcgcagcgt ttattttcca gatcatgccc gcggtcggcg
     3541 cgaccatgat cgcggtgcgc attttgacga tgcgtgcttc ctcgctcgcg agacgccccg
     3601 cggtttcgct gacatccgaa acgatgaact ggcgctgttg ggtcaggtct tgaagccgga
     3661 ttgtgatctc gtcttcgcgc tgttgcgaat aaggcgtgtc ggtcgaaccg ggatcggtga
     3721 tgatcccact tttgatcgcc gccagttgcg cctcaaaaga aataattcgg ccgcgctggg
     3781 cttctgcctc gttgaaagcc gtgccgaagt ccgctttagc cttatccaga tcggcggttg
     3841 aggagaagcc acgctctgcc aacaccgcac gacgatcgag cgtctgtttc gcttcatcgc
     3901 ggcgatagtt caacgccagg agctgatctt gggcttctgt caacgaaccc tgaatgcggg
     3961 ccgtgctcca ctctttgaaa taatcggcgc gaagctgtaa ggcagccctc aaagtttcaa
     4021 gggaattcat ttccgcggaa acgacggcta actcggcgcg cgcccgcgta aggtgctcgc
     4081 gtaaatcgac gaggtgcaag ttattgacgc ggtcgtctgt caccgtcgcg atgcgatcat
     4141 cttgatggac cggatcgccg acttttaatg gcatggcata aacgacgcca tcgataggtg
     4201 tccgcagggg aactgtatag gaagaaacga cggcgttgtc ggtcacgaca gcgaactcat
     4261 tggagagaat cgcgtaaccg ccgacggtca ggacggtcac cgtggcgccg agtttagcga
     4321 gtcgtagcca cggcaaatgg aaagttaagc cgacggcatt gggggcgggc tgaaccgagg
     4381 cccgtgctga aaattcactc attatccacc aataaagaaa taatttaagc agctacaaaa
     4441 cgcgctgtga aaagatgctg cgcgccaaat ctgaacagcg aataaactcg gcgatgcggt
     4501 tcaaaagtga gtaaaatcca agttaacgcc aatacgcgta gcgagtcgtt aggcttttga
     4561 ctccgtgaga ttgtgtgtgg gccagttaac tttgagcatc ccgggctagc tcatgccctc
     4621 gtgagctgat gcaggttact cagattgtgg aattttatgg cgacatttcg atccgtcttc
     4681 accacgagcg cgatcgccgc tgtcggggtg acgttgtgat ggtcgtatat tgtaattgcc
     4741 gaaggcgaga cgctccgacc gtcgcgtgat gcatctcaca cggattcgtc cggggctctt
     4801 tcgatgtgag tggtcgttcg ctgcaccgag cgcgctagcg gtcttccatc tgataaagca
     4861 ggcggatcaa gtcgaatgtg cgttcggcgc cgagcttgct tttgataatg ctgcaagcat
     4921 ttgccactgt cttgtgagaa acccccaaca ccagagcgat ggccccgaga gtctttccct
     4981 cggcgagcaa ccgggcgatg tcgatctcac gcgctgtaag cttctccagc gggttcgcgc
     5041 caccagatgg cgagagaacc atttgggtcg cgatctcacg ctctatatag cggccacccg
     5101 ctgcgaccgc ctgcacggca gcgatgattt ctttggtcga agcccccttg ctgacgtagc
     5161 ccagcgcacc ggcgataaat gtacgcgaca catagatcgg ctcgacatgc acgctaaaaa
     5221 cgacaatctt cgccagtcga tcttcgatca gcacattgct tagaagttca agaccgctgg
     5281 agttcggcag attaagatcc atcaggagga catcgggcgc tttcgagcgg tacaccgaca
     5341 aagcttcttg cgctgaggcg gcatcgaata tttccgtgcc cggcatggcc gcgaaaaggc
     5401 agcgcacccc ttcgcgcacc gcgagatgat cgtcgacaat caaaatcttc acgtcaccgg
     5461 ctccgtcggt cgccgtatgg gctcgctctg cccaaaacaa aaatggcgcc cgagccgaag
     5521 gacccgggcg ccattccagt catgcctaac ctgttttact ggtagacgcg gaaggtcgag
     5581 agcatgccgc cctgcgggat cagcgccaag ccggcggcct tggtcatggc cgtcgcgccg
     5641 atcgcaccga acttgtcgtt caggtcgagg ccggccgtga cgggcagacc gatccagcca
     5701 ccgatacccg agagcacgga cacatattgt ttgcccttga cctcgtaggt gatcgggttg
     5761 ccgatgatgc ccgagccgag cttgcgctgc cacaggattt cgcctgtgtc gcgatcgacg
     5821 gcgcgg
//
LOCUS       DASWOG010000358         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_44535,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000358 DASWOG010000000
VERSION     DASWOG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44535"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /gene="glnA"
                     /locus_tag="VGB93_07515"
     CDS             <1..1202
                     /gene="glnA"
                     /locus_tag="VGB93_07515"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0145985.1"
                     /translation="PDPNSAAVDPFFAAPTLSIVCDVLEPTTGQPYNRDPRGIAKKAE
                     AYRATTGVGDTVYFGPEAEFFVFDDVRFAAEPYNTGFELDSIEFPSNSDTPYEGGNLG
                     HRVRTKGGYFPVPPQDSAQDMRGEMLAAMASMGAKVEKHHHEVASAQHELGLKFGPLT
                     QMADHLQIYKYCIHQVAQSYGKTATFMPKPIYGDNGSGMHVHQSIWKGGKPLFAGNKY
                     ADLSDECLYYIGGIIRHARTLNAFTNPSTNSYKRLVPGFEAPVLLAYSARNRSASCRI
                     PYTSNPKAKRVETRFPDPTANPYLAFASLLLAGLDGIANKIDPGPAMDKDLYDLPPKE
                     LKKIPTVCGSLREALQNLDKDRAFLKAGGVFDDDFIDSYIELKMAEVQRYEMTPHPVE
                     FEMYYSL"
     gene            1358..>2007
                     /locus_tag="VGB93_07520"
     CDS             1358..>2007
                     /locus_tag="VGB93_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145986.1"
                     /translation="MHILINDFIGGVLDRGMPLYVRNLIDGLKAEGFRVNIVRAPRIC
                     RRLPRNVFYMLAVFVEQVIVPIIGFLKRAELTLYPYNSIAVIDIFTRRGRIVVHDLEQ
                     LNRPASLSKSYYLACYRALKWRDWPVFTISKLSQQRLRASDLLGDGPITILPNTFYAF
                     ERLLRIETQAPSDRASIMLCTGSTANKDIESVARDYLPKVLASGFHVSILGLHKTT"
BASE COUNT          427 a          621 c          534 g          425 t
ORIGIN      
        1 tgcccgatcc gaactctgcc gcggtcgatc ccttcttcgc cgcgccgacg ctatcgatcg
       61 tctgcgacgt tctcgagccg acgacaggcc agccctataa ccgcgatccg cgcggcatcg
      121 ccaagaaggc tgaggcctat cgcgccacga ccggcgtcgg cgatacggtc tatttcggac
      181 cggaagcgga atttttcgtg ttcgacgacg tgcgtttcgc ggccgagccc tacaataccg
      241 gcttcgaact cgactcgatc gaatttccgt cgaactcgga cacgccttac gaaggtggca
      301 acctcggcca tcgcgtacgc accaagggcg gttacttccc agtgccgccg caggattcgg
      361 cgcaagacat gcgcggcgaa atgctggcag caatggcatc gatgggcgcc aaggtcgaaa
      421 agcaccatca cgaagtcgcc tcggcgcagc acgaacttgg cctcaaattc ggcccgctga
      481 cgcaaatggc cgaccatctg caaatctata aatactgtat ccatcaggtc gcgcagagct
      541 atggcaagac cgcgaccttc atgccgaagc ccatctatgg tgacaacggc tcgggcatgc
      601 atgtgcatca gtcgatctgg aagggcggca agccgctgtt tgcgggcaac aaatatgccg
      661 atctctctga cgaatgcctc tattacatcg gcggcatcat ccgccatgcg cggacgctga
      721 acgccttcac caacccgtcg acgaacagct acaagcggct cgtcccgggc ttcgaggcgc
      781 cggtcctgct cgcctattcg gcgcgcaatc gctcagcctc ctgccgcatc ccctatacat
      841 cgaatccgaa ggccaagcgc gtcgagacgc gcttccccga tccgacggcc aatccctatc
      901 tcgccttcgc ctcgttgctg ctcgccggcc tcgacggcat cgccaacaag atcgatcccg
      961 gcccggcgat ggataaggac ctctacgatc tgccgccgaa ggaactgaag aaaatcccga
     1021 cggtctgcgg cagcctgcgc gaggccctgc agaacctcga caaggaccgc gccttcctta
     1081 aggcaggcgg cgtcttcgac gacgacttca tcgattccta tatcgaactg aagatggctg
     1141 aggtgcagcg ttacgaaatg acgccgcatc ccgtcgaatt cgaaatgtac tactcgcttt
     1201 aagcagcaag ccggacaaac gaaaggcgcc ctttggggcg ccttttttat tcgtccccgc
     1261 agtattaaga tttttcccgc atttgcctta aaaacttctc aagattaatc gcctaagtaa
     1321 gacgtcctga gcttttccac gcaatccggg accgggtttg catatcctca tcaacgattt
     1381 catcgggggc gtgctggatc gcggtatgcc gctttatgtc cgcaatctca ttgatggcct
     1441 gaaggcggaa gggtttcgcg tcaacatcgt gcgcgctccg cgtatttgcc gccgtctgcc
     1501 gcgcaatgtg ttctacatgc ttgcggtctt tgtggaacag gtgatcgttc cgataatcgg
     1561 atttctcaaa cgcgcggaac tgaccctgta cccgtataat tcgatcgcag tgatcgatat
     1621 ttttactcgg cgcgggcgga tcgtcgtgca tgatctggaa cagcttaacc ggccggcatc
     1681 gctttcaaaa tcctactatc tcgcgtgcta tcgcgcgctc aaatggcgcg attggcctgt
     1741 cttcacgatc tcgaaattga gccagcaacg attgcgagca agcgaccttt taggcgacgg
     1801 tccgatcacg attctgccga atacattcta cgcgttcgaa cggcttctta ggattgagac
     1861 gcaggcaccc agcgacagag cctcaataat gctgtgcacc gggtcgactg cgaacaagga
     1921 tatcgaatcc gtcgcgcgcg attatctgcc gaaagtgttg gcgagcggct ttcacgtgtc
     1981 gattctcggc ctgcataaaa cgacaga
//
LOCUS       DASWOG010000359         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_209661,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000359 DASWOG010000000
VERSION     DASWOG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209661"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..695
                     /locus_tag="VGB93_07525"
     CDS             162..695
                     /locus_tag="VGB93_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0145987.1"
                     /translation="MGLKAGHFFLSVGSMNKNKNISLAIEAARRLKRPDVPLVIVGGD
                     NNKVFQGGVTAAEYGIILAGRLRDEEIAALYACAIAFVFPSLYEGFGVPPLEAMLLGC
                     PVIASTADAVKETCGDAAAYFDPLDAEDLRRRMLERIAAGEISSDERNKQMQRIAAYS
                     WRNSATLLLNFLGNSAH"
     gene            788..1930
                     /locus_tag="VGB93_07530"
     CDS             788..1930
                     /locus_tag="VGB93_07530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0145988.1"
                     /translation="MPNEATGALKVLHVAETIRGGIATYFNELHPHQRACFGGDNVHY
                     VVPSDHRGDLIGFADTEVSVFERAGRSLQGLFRMAIVTLQQIRRVRPDIVHIHSTFAG
                     LVIRPILFLRRGGPQVVYCPHGWAFSRETSRLSHQITRLVELVLARITDRIVCISGDE
                     QNEAVNAGIARQRLILVHNGISANRPSPRTDLVPWVSKKTKVLFIGRLDRQKGYDLLV
                     ETARLLHETVEVRIIGSSVVGKHEDMEPPANVALLGWMDRSQIEAHLEAADLVVIPSR
                     WEAFGLVALEAMRAAKPIVAFRVGALPEIVEDGVTGILGSPVSVASLVDALTRASRLD
                     LQATGRHGYDRFRRLYDVKKTHQVLRELYQDITQDRRREITNIAMV"
     gene            2101..>2399
                     /locus_tag="VGB93_07535"
     CDS             2101..>2399
                     /locus_tag="VGB93_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145989.1"
                     /translation="MSNRSFLEETFRRRNFGQALAAIALTTSVAGCAGFLPLDAPDAV
                     ATKTNAALITAPSAPLPYALMPINADVLRATNSVTDDTGSAFAKLSSGNYRDVTIG"
BASE COUNT          500 a          688 c          661 g          550 t
ORIGIN      
        1 gccatttgct tgcgcgcaag gccacgattg cgacggtttc acaattttcg cgccgcgaac
       61 tcgcatcggt gctgtggttg cggcccgaat cgatacccgt attcccgaat agcgccgaac
      121 attttgccgc gaccgttccc gattttggaa tcatcgaccg gttggggctg aaagccggtc
      181 atttctttct ttcggtcggc tccatgaaca agaacaagaa tatttctctg gccattgaag
      241 ctgcgcggcg gttgaaacga ccggatgtgc cactcgtcat agtcggcggc gacaataaca
      301 aggtctttca aggcggcgta accgctgccg aatacggcat tatcttggcg ggccgcctcc
      361 gcgacgaaga aattgcagca ctctatgcct gcgcgatcgc ctttgtgttt ccgagtctct
      421 acgaaggctt cggcgttccg ccgctcgaag ccatgttgtt gggctgtccg gtcatcgcct
      481 cgacagccga tgcggtcaaa gagacctgcg gcgatgcagc cgcctatttc gatcccctcg
      541 atgctgaaga tcttcgtcgg cgcatgctcg aaagaatagc agctggtgaa atttccagcg
      601 acgagcgcaa taaacagatg caacgcatcg ctgcatattc atggcgtaat tctgcaacac
      661 tgcttttaaa ttttctcgga aactcagcac actgagaaat tgctcagtaa aaaattacgg
      721 cggtctaaat attgcttcat aggcacttga agagtctgct tccagtatct ggatcagaaa
      781 gtttccaatg ccgaatgaag cgacaggcgc gctgaaagta ctccatgttg ccgaaactat
      841 tcgtggcgga attgcgactt acttcaacga gcttcatccc catcagcgcg cctgcttcgg
      901 cggtgacaac gtacattatg tggtgccgtc agatcatcgc ggcgatctga taggctttgc
      961 ggacaccgaa gtgtccgtgt tcgagcgcgc gggccgaagc cttcagggtt tattccggat
     1021 ggcaatcgtg acgctccagc aaattcgccg cgtccggccc gatatcgtgc atatccattc
     1081 gacatttgca ggcctcgtca tacggcccat tctgtttttg aggcgcggcg gaccgcaggt
     1141 ggtttactgc ccgcacggct gggctttttc gcgcgaaacc tcgcgactca gtcatcagat
     1201 cacaaggctt gtcgaactcg tgctcgccag gattaccgat cggattgtct gcatctccgg
     1261 cgatgaacag aatgaggccg tcaatgccgg tattgcccgc cagcgcctga tcctagtcca
     1321 caacggcatc tcggccaatc gcccgtcacc ccgaaccgat ttggtcccct gggtttcaaa
     1381 aaaaaccaag gttcttttca tcggccgcct cgatcgccag aagggatacg atctcctggt
     1441 cgaaaccgcg cggcttctgc atgagacggt agaggtccgc atcatcggtt catcggtcgt
     1501 cggcaagcac gaagatatgg agccgccggc gaatgtggcc cttttgggat ggatggaccg
     1561 ttcgcagatc gaagcgcatc tcgaggctgc cgatcttgta gtcattccgt cgcgctggga
     1621 ggcattcggc ctcgttgcac tcgaggcgat gcgcgcagct aaaccaattg tcgcttttcg
     1681 cgtcggcgcg ttgcctgaaa ttgtggaaga cggcgtaacc ggcatcctgg gcagtccggt
     1741 ttcagtggca tcgctggtcg atgcgctcac gcgcgcatcg cgactcgatc tgcaagcgac
     1801 cggccgccat ggttatgacc gtttcaggcg actctacgac gtaaaaaaga cccaccaggt
     1861 gcttagggaa ctttatcagg acatcacgca ggatcgacgt cgcgaaatca ccaacatcgc
     1921 gatggtttag cgtgccgtct aattaccgaa aactatttga gggggaattc cgaaccagcg
     1981 agttgcatca aagtcacatc gcgccgctta cgcgctgcgc tgcgttgaac tgtcgtgact
     2041 ctgactcgat ctcgctgcta ggttttttgc acaacggggg cgtagctagt gaccatgcag
     2101 ttgagtaaca gatcgtttct tgaggaaaca tttcgtcgcc gaaattttgg ccaggcttta
     2161 gccgctatcg ccctgacgac gtcggtcgcg ggatgtgcgg gcttcctgcc gctggacgcc
     2221 cccgacgctg tcgcgacaaa aaccaacgcg gcgctgatca cggcgccgtc ggcgccgctt
     2281 ccttatgcac tgatgccgat caatgctgac gtgctacgcg cgacgaattc cgtcaccgac
     2341 gacaccggct cggccttcgc aaagctgtcc agcggcaatt atcgcgatgt gactatcgg
//
LOCUS       DASWOG010000360         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_302191,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000360 DASWOG010000000
VERSION     DASWOG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302191"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..938
                     /locus_tag="VGB93_07540"
     CDS             396..938
                     /locus_tag="VGB93_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0145990.1"
                     /translation="MLAVTLTGFSGLTAQATMMSSDPMVGGAAMYPSRNIVQNAVHSK
                     DHTTLVAAVKAAGLVDTLEGAGPFTVFAPTNEAFAKLPPGTVATLLKPESKSTLVSVL
                     TYHVVAGRYSARQLKEMVKMGGGQAMLKTVEGGQLTVKEYDGKLELIDAKGDKAWITI
                     SNVNQSNGVIHVINTVLLPG"
     gene            1182..>2096
                     /locus_tag="VGB93_07545"
     CDS             1182..>2096
                     /locus_tag="VGB93_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0145991.1"
                     /translation="MTSTSTYAPSREDFTALLDESYGHNEALEGAVIKGIVVAIEKDV
                     AVIDVGLKTEGRVALKEFQGPGREGTLNVGDEVEVYLERIENALGEAVISRDKARREE
                     SWVRLERAFEKQEKVEGVIFNQVKGGFTVDLDGAVAFLPRSQVDIRPIRDVTPLMHVT
                     QPFQILKMDRRRGNIVVSRRTVLEESRAEQRHEIVANLEEGQVIDGVVKDITDYGAFV
                     DLGGIDGLLHVTDIAWRRVNHPSEVLTIGQTVKVKIITIKHETHRISLGMKQLLDDPW
                     QGIDAKYPVNARIHGRVTNITDYGAFVEL"
BASE COUNT          439 a          643 c          607 g          407 t
ORIGIN      
        1 ttcaaaaata cgtcttggag cgcctcttcc gccatgctcc tgctgggaag aatacgcacg
       61 agaatcgcaa aaagtttcgg tgcggtgcgc tgataaagtt cggcgtaggc cgcacggtcg
      121 ccacgcgccg ttgcaacgag gaaggtgacg agctcgctat tgtccaatcg cgtcaatgcg
      181 gcaaccctgt ctttgcaccc aatcttcctt aacgacggat acgaaacaaa gtctaggcgc
      241 gtgccaacgc gtgcgaaatt cttttcaaat cgcgaaaccc gttttgatcg ccttccgtag
      301 atgaaagtga cgcgcatggc ggggccagtt gagttcccgc cggcggtgtt cgctcaagga
      361 gacgaattat gaagttctcg aaactcaatg ctctcatgct cgccgtaacg ctgaccggat
      421 tttccggcct cacggcgcag gcgacgatga tgtcctccga tccgatggtc ggcggcgcgg
      481 cgatgtaccc atcgcgcaat atcgttcaaa acgccgttca ttcgaaagat cacacaacct
      541 tggtcgccgc cgtgaaggca gccggccttg tcgacacgct cgaaggtgct ggcccgttca
      601 cggtcttcgc gccgacgaac gaagctttcg ccaaattgcc gccgggcact gtcgctactc
      661 tgctcaagcc cgagagcaaa tccaccctcg tgtcagtttt gacctatcac gtcgttgccg
      721 gccgctattc ggcccgccag ctcaaggaaa tggtcaagat gggcggtggc caggctatgc
      781 tgaagaccgt tgagggtggg cagctgacgg tcaaggaata tgacggcaag ctcgaactca
      841 tcgacgccaa gggcgacaag gcctggatca ccatctccaa cgttaaccaa agcaatggcg
      901 tcattcatgt gatcaacacg gtgcttctac cgggctgatc gcctcggcgg acccggttca
      961 aggtcgattt gcagttcccg cacgtctctt gccgcgcgcc ccttgcccag ggcgcgcgcg
     1021 cttgctacat agccgcccga acctagcttt tttccgccgt ccgacgccgg tccgcctttt
     1081 tgatgcgggt gatcctgtcc ttgggcaggc cacggtggga agaagctccg tcaaccttta
     1141 accgccggcg ttttgcccga ttgggcattt caggagaacg aatgacatct accagcacct
     1201 acgcgccgtc gcgcgaggat tttaccgcgc ttctcgatga gagctatggc cacaacgagg
     1261 ctctcgaagg cgcggtcatc aagggcatcg tcgtcgccat cgaaaaggat gtcgccgtca
     1321 ttgacgtcgg cctcaagacc gaaggccgtg tcgccctcaa ggaattccag ggtccgggcc
     1381 gcgaaggcac gctgaacgtc ggtgatgaag tcgaagtcta cctagagcgc atcgaaaatg
     1441 cgctgggcga ggcggtgatc tcgcgagaca aggctaggcg cgaagagagc tgggtgcgcc
     1501 tcgagcgcgc cttcgagaag caggaaaagg tcgaaggcgt catcttcaat caggtcaagg
     1561 gcggcttcac cgtcgatctc gacggcgccg tggccttcct gccgcgcagc caagtcgata
     1621 tccggccgat ccgcgacgtc acgcctttga tgcacgtcac gcagccgttc cagattctca
     1681 agatggatcg ccggcgcggc aatatcgtcg tctcccgccg caccgtgctt gaagagagcc
     1741 gcgccgagca acgccacgag atcgtcgcca acctcgaaga gggtcaggtc atcgacggcg
     1801 tggtcaagga catcaccgat tatggtgcgt tcgtggacct cggcggcatc gacggcctgt
     1861 tgcacgtcac cgacattgcc tggcgccgcg tcaatcatcc gagcgaggtt ctgaccatcg
     1921 gccagacggt caaggtgaag atcatcacga tcaaacacga gacgcaccgc atctcgctcg
     1981 gcatgaagca attgctcgac gatccgtggc agggcatcga cgccaaatat ccggtcaatg
     2041 cccgcatcca tggccgcgtg accaacatca ccgactacgg cgcgttcgtc gaactg
//
LOCUS       DASWOG010000361         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_320303,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000361 DASWOG010000000
VERSION     DASWOG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320303"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..538)
                     /locus_tag="VGB93_07550"
     CDS             complement(269..538)
                     /locus_tag="VGB93_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145992.1"
                     /translation="MFQGHFDLDTATPVDAHAIPDVLFERGLYWASGRSGVVNLIAAH
                     KWFNLAAVKGRAGAIAMRREVAEMMSDVEIATAQREARAWITTQQ"
     gene            complement(1942..>2203)
                     /gene="gatB"
                     /locus_tag="VGB93_07555"
     CDS             complement(1942..>2203)
                     /gene="gatB"
                     /locus_tag="VGB93_07555"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441330.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY0145993.1"
                     /translation="DLFEIVWQEGGDPRALVEARGMRQVTDLGAIEKVVDDILAANPD
                     KVAQAQAKPALMGWFVGQVMKSSGGKANPQAVNDLLKAKLGI"
BASE COUNT          458 a          665 c          621 g          459 t
ORIGIN      
        1 atatggatcc gtcgtggcgc cttcggtcgg atcttaccag ggcacggtag gctcttctaa
       61 cgtagcgccg acatggagaa acaccggccc cgcagcgatt acgcggagtt cttcggcgcg
      121 tgccgccagg caggcgcgca ccggtatgac agtcagtaga tagaacgctg cggcatacgc
      181 cgctggcgtt ggcggcttcc ggactgcgca cgagcggcgg gaagtgaagc aacaagcgaa
      241 ggggcgcgat aacccctccc ctgaaagctc attgctgggt tgttatccag gcccgggctt
      301 cgcgctgggc ggtggcgatt tcgacgtccg acatcatctc ggcgacctcg cggcgcatcg
      361 caatggcacc ggcgcggcct ttcacggcgg cgaggttgaa ccatttatgc gcggcgatca
      421 ggttgaccac cccggagcgg ccgctggccc agtacaagcc gcgctcgaac aacacatccg
      481 ggatcgcgtg cgcatccacc ggcgttgcgg tatccagatc gaaatgcccc tgaaacatct
      541 gttatcccct tttttgattt tttggccgcg ctgtcccgag cgcggccccc gtccaactgt
      601 taaaaacgct ttgctatccc aaccgccgtt tgccggcttg ttgggatcat catgacccat
      661 caaatttgaa gagcagttta agtatcggga tgaatgcggc gtaaacgcac gcacggcgcg
      721 cacgccggag aaattgcaaa agccgctgtt ttgcagggct ctgctcattt ctggaatttg
      781 gtttaccgtg ccatttggcg aaacgataac catcggccgc gaacgcgtgc cgaggcgcca
      841 acgcacaaac cgccccggct ttggggccgt ggctgatcag gacagacgtt aagggggatt
      901 ttgaaggtgg gatttggcgc tgtaccgggc gctatcggca ccggcaggga acgagggcgt
      961 cggcgaacac gtcaggacca gatctcaatc gggctttgcg acccggtgaa attgcggacc
     1021 tcaagatccg caccgaacga agaaaacttg tttcttctgc cggaccggtt tcaagaagcg
     1081 aactccctaa aatgacgatc cagccgtttg acctgatgtc tcgccgacac cctctttcgt
     1141 cgaccagagc ctgctttcaa aatccgcttc gagaagcaaa ctatcctgaa ggctcgtgtg
     1201 acgtgccggc atcaagccgc cggcaatttt tcagaagcga gattacttct tcttcttggc
     1261 gaccttgcgg gtcttcttcg cagtcttctt cactgcgctc ttcgccttct ttgccttctt
     1321 agctttcttg gccatgttgc cctcctaatg tagtgagatg gcttaatcgc tgcgtgcact
     1381 cgggaatcga aatgcactac atcccgaata caccaacgca tctaaaaaaa cagcttctcg
     1441 cttaaggaag tgttgacgca gcgatggcag agcgcgcgac gcgtgctcgt cggcaacacg
     1501 acaacgcatc cacacaacga tgcaagtagc ctgcggaaac gcaacacttg cgaatgtcaa
     1561 cagcacaaga aactactgtg ctgcaggtgt tttcgcaatt cacgcattga tcgcgcgaca
     1621 cgaattcgac gtcgtcgcga ctcatcaacg cccgaaaaaa cgcgccgcac ggactctgag
     1681 tcgcgatttt tggcaatgaa aaaattttcg tgctcaccgg cctcgcgtct catcggtgct
     1741 cgccaaaacc ggatttttgc gagaattgcc gcggactgat tcgcatccgc gattgcggga
     1801 tcgcgcccga tgcatcgccg cttatggtga ataattgatg cacacgcgcg ctcacgagca
     1861 tgtcgacggc gacgccaaca gcgtcacgtt gacaacgaca gcgtcgcatc gggacgacga
     1921 agacgtcaca tcgcgcgcaa ttcagatgcc gagtttggcc ttgagcagat cgttgacggc
     1981 ctgcgggttg gccttgccgc cggatgattt catcacctga ccgacgaacc agcccatcaa
     2041 cgccggcttg gcctgggctt gcgcgacctt gtcgggattg gccgcgagga tgtcgtcgac
     2101 caccttttcg atcgcgccga ggtcggtgac ctgcctcatg ccgcgcgctt cgaccagcgc
     2161 gcgcgggtcg ccgccctcct gccagacgat ctcgaacaga tcc
//
LOCUS       DASWOG010000362         6140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_136882,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000362 DASWOG010000000
VERSION     DASWOG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136882"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VGB93_07560"
     CDS             <1..271
                     /locus_tag="VGB93_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0145994.1"
                     /translation="RAVDRDTGEILWQRKLGSGIIGNPITYEVKGKQYVSVLSGIGGW
                     IGLPVTAGLDLSDKFGAIGATAMTKATGLAMIPQGGMLSTFRVYQ"
     gene            429..2249
                     /locus_tag="VGB93_07565"
     CDS             429..2249
                     /locus_tag="VGB93_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEY0145995.1"
                     /translation="MKPFLLKSLASAFCLSAMALPLMVFTPAAATQDSEARLDHAVAV
                     ALSKLDALQPLTSGDRAALKAAARKRQLNSLRLCGDPGNMPLSNDKLEGYENKIGEIL
                     AKAMNTHTEYYWRPYIERGMLRETFGNDQCDVLMDLPLGYEGVLLTEPIYKTTFVLVS
                     RADRKYNFKSLEDPELAKLKIGVYQTSAIRQVFHDHGMDKSLVVHTISHDADLNPEDQ
                     PAYQVQQVIDGKLDIAAVWGPFAGYYKAMKNAPINIQPINLMEDQIPLEYELAFGVPT
                     TDRILKYKIDLALEDNKVAIEKVLRDYGVPLVQCSDCVVQGDLPAHGTYSALPPTPPS
                     ELKSSVTIKQLQSWLDAGADVNEEFNNAVTAGSVDRAAFLLGKGANPNKPDDQGETPL
                     INSVLAQNPDLVKLLIERGAKVNGRDSNGMTALLQAINLDDVAAIKVLAAHSANLNLP
                     SSDGATPLAFAIVENKMKAALALIDAGAAINAPSGELGLTPLMVTSGRQETELSLEAG
                     RHEVENVSGDAHYPDPLQVARVLIAHKADVNAVSKSGVTALMLAAAHNNPPVVGLLLQ
                     SGADPSKRTLDKKTALDLAVENGNDSVVSLIRLIQQSNGK"
     gene            2530..2967
                     /locus_tag="VGB93_07570"
     CDS             2530..2967
                     /locus_tag="VGB93_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0145996.1"
                     /translation="MSARADDTANESPTKVATDATKVGSLVNPYQAKEDAHDQALADE
                     GHKLFGNFGCPGCHGYGGGGGMCPPITNGVWIYGDKDDTLFRLISLGSDALQKEGFTR
                     QATENIVAPMPPMGGVITNADDLWKIIAWIKIQAAANVKANPQ"
     gene            3072..4364
                     /locus_tag="VGB93_07575"
     CDS             3072..4364
                     /locus_tag="VGB93_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0145997.1"
                     /translation="MKHAGVLTVFRRVSRSYRRPLLPLVASAVSFVCVTGVAVAQSAA
                     PPASPAQTQLAVPASLNVDILYLGRQYNEPPPLSLLDKIVTDQGIQGARIALLENAQT
                     GRFVNQNYHLSEMILPLDADFKAKARAALATHKLIIADLEKDDLLALADMPEAKDAII
                     FDIRTSDDGLRQEQCRSNVFHFLPNTAMRTDALAQYLVWKHWPNWLLVSGKQPADIAY
                     ADDVKQSAHKFGAKVVDERFYTYAAGSRRTDTGHQQVQQQLPLVTQSAPPYDVVFVAD
                     DSQVFGEYMPYRTWEPRPVVGTAGLIATAWARAYEEQGGTQLQNRFERKVGRIMTERD
                     YSAWLGIRAFGEAILRSNTIQLAALRTYLLSDRFGVAGYKSESMNFRKWDQQLRQPVL
                     LTSARDLVSVSPQEGFLNPNYLTDTLGFDQPESKCHFK"
     gene            4380..5360
                     /locus_tag="VGB93_07580"
     CDS             4380..5360
                     /locus_tag="VGB93_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEY0145998.1"
                     /translation="MRKIREFFFFGLAALLTFAAVGQANAAMIFVSNEKDNTVSVIDS
                     TSLKLVTTIDVGQRPRGIIITPDHKTIVVCLGDDGQIAMIDANTFKVTRKFDSGSDPE
                     LLNISPDGKILYVANEDDSLVTIMDFATGKTIQEIPIGVEPEGMAVNPSGNLVVATSE
                     STSMAHFIDAKTYKVLANVLVDSRPRYAEFTADGSKVWVTSEVGGTVSVIDAKTFKIL
                     NAVHFDVQGVRPELISPVGITFTKDGKRAFISLGKANRLGVVDTSNYNVVGYVLVGQR
                     PWHTALSPDGATIYSANGESNDITAVDVASLRPLKSVPVGRLPWGLTIRP"
     gene            5394..5774
                     /locus_tag="VGB93_07585"
     CDS             5394..5774
                     /locus_tag="VGB93_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0145999.1"
                     /translation="MPGKKSKPALVSYLAAVGALCAIVFAAPDVQAAPKTKFWNTTGD
                     TIVKFELAPAGTTTWGPDQCKNDRDDSVDDDERLLIVGVASGTYDARMTYKNGAVCMA
                     KSVKVVEGKIFSIEKKDLTNCTKP"
     gene            5832..>6140
                     /locus_tag="VGB93_07590"
     CDS             5832..>6140
                     /locus_tag="VGB93_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146000.1"
                     /translation="MSNNKSVGGRRGPHNISGALSALVSVCSIVALGLAFTITRGATD
                     PVKPPYIQFIGHTADLTDAEYSADGKRVLTASSDKTARIWNAANGVSLITLSGHTAAV
                     R"
BASE COUNT         1322 a         1786 c         1775 g         1257 t
ORIGIN      
        1 ccgcgccgtc gatcgcgaca caggcgaaat cctgtggcag cgcaagctcg gctcgggcat
       61 catcggcaac ccgatcacct acgaggtcaa gggcaaacaa tatgtgtccg tgctctcggg
      121 tatcggtggc tggatcggtc taccggtcac ggccggcctc gatctcagcg acaagttcgg
      181 tgcgatcggc gcgacggcca tgaccaaggc aactggcctt gccatgatcc cgcagggtgg
      241 catgctctcg accttccgcg tctaccagta agaccggtta gggtataaat agaatggcgc
      301 ccgggtcctt cgactcgggc gccatttgta tttcggccga aaggctctga ccaagctccg
      361 cttgaccaat atcccgaccg atataaagcg tcgcctaagc gaacgttgcc atgaggaagc
      421 cgctcacgat gaaaccgttt ctgcttaaat ctctcgcctc ggccttctgt ctgtcggcaa
      481 tggcgctgcc cctgatggtc tttacccccg cggcggcgac gcaggatagc gaggcgcgcc
      541 tcgaccatgc cgttgcggtg gctctcagca agcttgacgc gcttcaacct ctgacgtcgg
      601 gtgatcgcgc ggcgttgaag gccgcagccc gcaaacgcca gctcaattct ttgcggcttt
      661 gcggcgaccc cggcaatatg ccgctctcga acgacaagct cgagggctac gagaacaaga
      721 ttggcgaaat tctcgcgaaa gcgatgaata ctcataccga atattattgg cgcccctata
      781 tcgagcgcgg catgttgcgc gagactttcg gcaacgacca atgcgacgtg ttgatggatc
      841 tgccgctcgg ttacgaaggc gttctgctca ccgagccaat ctacaagaca accttcgtcc
      901 tcgtctcgcg cgcggaccgc aaatacaatt tcaagagcct tgaagatccc gaactcgcga
      961 agttgaagat cggcgtttat cagacctccg ccatacgtca ggttttccac gaccatggga
     1021 tggacaagag cttggtcgtc cacaccatca gccacgatgc cgatctgaat ccagaggatc
     1081 agccagccta tcaggtccag caggttattg atggcaagct cgacatcgct gccgtctggg
     1141 ggccgtttgc gggttattac aaggcgatga aaaacgcgcc gatcaatatc cagccgatca
     1201 atctgatgga agatcagatt cccttagaat acgaactcgc ctttggtgtc ccgacgacgg
     1261 accgcattct caaatacaag atcgatctag cgcttgagga caacaaggtt gcgatcgaga
     1321 aagtgttgcg cgattacggg gtgccgctcg ttcaatgcag cgattgtgtc gtccagggcg
     1381 acctaccggc gcatggcact tattcggcac tcccgccaac gccgccatcg gaactgaaat
     1441 cttcggtcac gataaagcag cttcagtcct ggctggatgc cggcgccgac gtgaacgaag
     1501 aattcaacaa cgcagtgacc gctggttcgg tcgatcgcgc ggcattcctg ctcggcaaag
     1561 gcgccaatcc caacaagccc gacgatcagg gcgaaacgcc gctgatcaat tcggttctcg
     1621 cgcaaaatcc cgatctagtg aaactgctca tcgaacgcgg cgccaaagtg aacgggcgcg
     1681 attcgaacgg catgacggcg ctactgcagg ccataaatct cgacgatgtc gcggcgatca
     1741 aagtgctggc ggcgcacagc gccaacttga acttgccttc ctcagatggc gcaacgccgc
     1801 ttgcgtttgc gatcgtcgag aacaagatga aagcggcact ggcgctcatc gacgcgggtg
     1861 cagccatcaa cgcacctagc ggcgaactcg gcctgacgcc tttaatggtg acttccggac
     1921 ggcaggagac tgagttgtcg ctcgaagccg ggcggcacga agtcgaaaat gtctcaggcg
     1981 atgcgcatta tccggacccg ttgcaggtcg cgcgcgtgct tatcgcgcac aaggccgatg
     2041 tcaatgccgt cagcaaatca ggcgtgacag cgctcatgct ggcggcggcg cataacaatc
     2101 cgccggtcgt cggtctgttg ctgcaatcgg gcgctgatcc gtcgaaacgc actctggata
     2161 aaaagacagc gctcgatctt gccgtcgaaa acggtaacga ttccgtcgtg agccttatcc
     2221 gcttgatcca gcaatcgaac ggtaagtaat tggatttgtt ccagttctga tgttcagcgt
     2281 gcatcagggg cggaacttcc aatgaagtca aggcgttctt ggcatagaag ataggcaaac
     2341 gcagcgcttg acgttttttc ggggctggcg gaaaaggccc atcgtcgccg gttttttttc
     2401 gcgcgatcgg ggattttctg ggaattcgat ggaagacgtc ctgccgtgca ggatcaagga
     2461 ggggttatgg tcaaaggtgc agtaaaggtt gttgtgggcc tgtgtttggc ctttggcctg
     2521 actttcgcga tgagcgcgcg cgctgacgac acggcgaacg agtcgccgac caaagtggcg
     2581 acggacgcga caaaggtcgg ctctctcgtc aacccctatc aggccaagga agatgcgcac
     2641 gaccaggctc tggccgacga aggtcataaa ttgtttggca atttcggttg cccgggctgt
     2701 cacggttacg gcggcggcgg cggcatgtgc ccgccgatca ctaacggcgt ctggatttat
     2761 ggcgacaagg acgacacgct gttccgcctg atctcgctcg gttcggacgc ccttcagaag
     2821 gaaggcttca cgcgtcaggc gaccgaaaat atcgtcgcgc cgatgccgcc gatgggtggc
     2881 gtgatcacca acgcggacga tctctggaag atcatcgcct ggattaagat ccaagccgcc
     2941 gcgaacgtga aggctaatcc tcaataatcg tggaaaacgc ataccgaagt tgccagcact
     3001 aaagcgcggc gagggagagc gggtacgatc tcgctctccc agtaaaatat aagaggtagc
     3061 gggagacgcg ggtgaagcat gcgggggtgt tgaccgtgtt tcgacgcgta tcgcgtagct
     3121 atcggcgacc gctccttccg ctggttgcca gcgccgtttc tttcgtctgc gtcactggcg
     3181 tcgcggtcgc gcaatcggcg gcccctcccg cgagcccggc ccagacccag ctcgccgtgc
     3241 cagcgtctct caatgtcgat attctctatc tcggccggca gtataacgag ccgccgccgt
     3301 tgtcgctgct cgacaagatc gtcaccgacc aaggcattca gggcgcccgc atcgcgctcc
     3361 tggaaaatgc gcagaccggc cgtttcgtga accagaatta tcatctcagc gagatgatcc
     3421 tgccgctcga tgcagatttc aaagccaagg cgcgcgctgc gcttgcgacg cataaactca
     3481 tcatcgccga tctcgaaaag gatgatcttc tggcgctcgc ggatatgccc gaggccaagg
     3541 acgcgatcat tttcgatatc cgcaccagcg acgacggtct gcgccaggaa caatgccggt
     3601 cgaacgtctt ccatttcctt ccgaacacgg ccatgcgcac cgacgccctg gcacaatatc
     3661 tcgtctggaa acattggccg aattggctgc tcgtctcagg aaaacagcct gccgatattg
     3721 cctatgcgga tgacgtcaag caatcggcgc acaagttcgg cgctaaggtc gtcgatgagc
     3781 gcttttacac ctatgctgcg ggctcgcggc gcaccgacac gggccaccag caggtccagc
     3841 agcaattgcc gctcgtcaca cagagcgcac cgccttatga cgttgttttc gtcgccgacg
     3901 acagtcaggt cttcggcgaa tatatgccct accgcacctg ggagccgcgt cctgttgtcg
     3961 gcacggcagg tctgattgcg acggcctggg ctcgtgccta tgaggaacaa ggcggcacgc
     4021 aattgcagaa ccgcttcgag cgcaaggttg gtcgcatcat gaccgagcgt gattattccg
     4081 cgtggctcgg catccgcgct ttcggcgagg cgattcttcg ctcgaatacc attcagctgg
     4141 ccgcgctgcg cacttatctt ctgtcggatc gcttcggcgt tgcgggctac aagtcggaat
     4201 cgatgaattt ccgcaaatgg gaccagcaat tgcgccagcc ggtgttgttg acctccgcgc
     4261 gcgatcttgt ctcggtctcg ccgcaggaag gatttttgaa tccgaattat ctgacggata
     4321 cgctcggttt tgaccagccg gaaagcaaat gccacttcaa ataaggacga aggcgggcga
     4381 tgcggaaaat tcgtgaattc tttttcttcg gtctcgctgc tcttttaacc ttcgcagcgg
     4441 tcgggcaagc caatgctgcg atgattttcg tgtccaacga aaaggacaac acggtttcgg
     4501 tcattgacag cacgagtctg aaactcgtga cgacaattga cgttggccag cgtccacgcg
     4561 gcatcatcat cacgccggat cacaagacga tcgtcgtctg cctcggcgat gacggccaga
     4621 tcgccatgat cgacgccaac acgttcaagg tgacgcgcaa gttcgattcc gggtccgacc
     4681 ctgaactcct caacatctcg cctgatggca aaattctcta tgtcgccaac gaggacgaca
     4741 gcctcgtcac gatcatggat ttcgccaccg gcaagacgat ccaggaaatt ccgatcggcg
     4801 tcgagccgga agggatggct gtcaatccta gcggcaacct tgtcgttgcg acatccgaat
     4861 cgaccagcat ggcgcatttc atcgacgcca agacctataa ggttctggcc aatgtgcttg
     4921 tcgattcgcg accacgttac gcggagttca ccgccgacgg ttcgaaggtc tgggttacat
     4981 ccgaggtcgg cggcaccgtt tcggtgatcg acgcgaagac gttcaaaatt ctcaacgccg
     5041 ttcatttcga tgttcagggt gtgcggcctg agctgatttc accggttggc atcaccttca
     5101 cgaaggatgg caagcgggcc ttcatcagcc tcggcaaagc caatcgcctc ggtgttgtcg
     5161 atacgtcgaa ctacaatgtc gtcggctatg tgcttgtcgg acagcgtccg tggcatacgg
     5221 ctctcagccc tgacggtgca accatctatt ccgccaatgg cgagagcaat gacatcaccg
     5281 ctgttgatgt cgcctcgttg cggccgctga aatcggtgcc ggtcggccgt cttccgtggg
     5341 gattgacgat caggccgtag aactgacgca aaataaaacg caagggacgg aaaatgcctg
     5401 gaaagaagtc gaaacccgcg ctggtttcat atctcgccgc cgtcggggcg ctttgcgcaa
     5461 ttgtctttgc cgctccggat gttcaggccg cgccgaagac aaaattctgg aacacgacgg
     5521 gcgatacgat cgtcaaattt gagcttgcgc cggccggcac cacgacctgg gggcccgacc
     5581 aatgcaagaa cgatcgtgac gattcggtcg atgacgacga gcgcctgctg atcgtcggcg
     5641 tggcgagcgg cacctatgat gcccggatga cctacaaaaa cggcgccgtc tgcatggcga
     5701 agtccgtcaa ggtcgtggag ggcaagattt tctctatcga gaagaaggat ttgacgaatt
     5761 gcaccaagcc gtaattcggc gatgaccgaa ggcgaggtgg caaagaggtc ccagcaaaaa
     5821 ggaccaagcg aatgtccaat aataaatctg tcggcggccg ccgggggccg cacaacatct
     5881 ccggtgcttt gagtgctctg gtatctgtct gtagtattgt tgcattaggt cttgcgttca
     5941 cgatcacgcg cggcgccacc gatccggtca agccgcctta tatccagttc atcggccaca
     6001 cggccgatct caccgacgcg gaatacagcg ccgacggcaa gcgcgtgctg acggcttcga
     6061 gcgacaagac ggcgcgaatc tggaatgcag ccaacggcgt ctcgctgatt acgttgagcg
     6121 gccatacggc ggccgtgcgc
//
LOCUS       DASWOG010000363         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_246740,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000363 DASWOG010000000
VERSION     DASWOG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246740"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VGB93_07595"
     CDS             <1..136
                     /locus_tag="VGB93_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146001.1"
                     /translation="PVAERLFFAGEATHPFFFSTVHGAWESGLRAAHEVLTVLPAPIL
                     "
     gene            219..983
                     /locus_tag="VGB93_07600"
     CDS             219..983
                     /locus_tag="VGB93_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146002.1"
                     /translation="MAILIILVVGLAAAAVTLVVQKAVAADLRREHHEVGSTVFLQLG
                     VIFAVLLAFVFSEAWTEYNEASAAIDLEVAALHGAAMLAATLPAPAAKEILSKEQAYL
                     DSVVQSEWPIMAAHRSEDILTDHKLRALIQAAAGLKFNDPAESYTKPEILSLLTQAHA
                     QREVRIYQAGSGIPGALWTVLIGFEAILVTFVVLSGLRFWTTAALVSGIFAASVSSIL
                     VVARLLDYPFEGALALGPHDFIAVTKKVTELLVSGG"
     gene            1105..2745
                     /locus_tag="VGB93_07605"
     CDS             1105..2745
                     /locus_tag="VGB93_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0146003.1"
                     /translation="MSAPVFITHSSRDYKQSRAIVDVLEKNGIPCWISERDIGAGDNY
                     GDAIVDAIENAKAMVLVFSVNANNSDEIKKEIALASQRKLTVVPIRIEDATPSKAFRY
                     ELITRNWIDAFQDWNQAMETLTRRVSVIVNGAEARLPEPAPKPKPTPGISPAMIAAVV
                     AGVAIVGGGAAYGLWPRPPSPIIIVHNDDTIHQKPAPAPQPPPASPTEPTADKTAVVV
                     QGGGETAPAVKPAALNPAPAAKLASPSPAAKKTVVAALIPMVPAEKIAPPAAKPAAPA
                     IAPAPPPYNLATNGGQVFQECKNCPQMVVVPRGAATLGSPAGEPGRQSNELTPHGVTI
                     GAPFAVGRYAVTFDEWDACVADGGCNGYKPDDNGFGRGKRPVIFVSWNDANAYVKWLK
                     SKTGALYRLLSEAEWEYAARGCTTAECPNTPFWFGDISPELANYDSRYSYEGSTKADR
                     ALETVPVDQGKPNPFGLTNILGNVQQWVEDCWNAQLDAVPADGGPVLTGDCSDRVIRG
                     GSYSDKPDALRAASRAWDAADDRESPNVGFRVGRSLTP"
     gene            2802..>3394
                     /locus_tag="VGB93_07610"
     CDS             2802..>3394
                     /locus_tag="VGB93_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0146004.1"
                     /translation="MLRLRMHQAVRALACLGFAGLCVFGAVSGAQAETRRAFVLGVQR
                     YSDSDIQSLTRSDADAADIAGDLEDLGFDKKNVTLATDLRTKDDFDKKFQAFLKTIQE
                     GDDVVFFFSGHGVGVEATNTNYLLFSDLKSLKTYTKSQLLESDRRDDIIALKMPSFQG
                     QYETEEIPKDGVSASEIIQQIAEKKPRVAFIVLDACRS"
BASE COUNT          676 a         1026 c         1025 g          667 t
ORIGIN      
        1 gccagtcgcg gagcggctgt ttttcgccgg cgaggcgacc catccgttct tcttttcgac
       61 cgtacatggc gcatgggaga gcgggcttcg cgccgcgcat gaggtgctca ccgtgctacc
      121 ggcaccaata ctttgacaaa gccggttcga cagtttcgga taagttgcag acggtaaatt
      181 tggcctccgg ggggatctca tctcgcatga gttatagcat ggccatcctg attattttgg
      241 ttgtagggct ggctgcggcc gcagtgacgc ttgtcgtcca gaaagccgtt gcggccgatc
      301 tgcggcgcga acatcacgag gtgggttcga ccgtcttcct gcagctcggc gtcattttcg
      361 ccgttcttct cgcattcgtc tttagcgagg cctggaccga atataatgaa gcgtccgcgg
      421 ccatcgatct ggaagttgcc gccctgcatg gcgccgcgat gctcgctgcc acgttgccgg
      481 cgcccgccgc aaaggagatt ctgagcaagg aacaggccta tctcgacagc gtcgtgcaaa
      541 gtgaatggcc gatcatggcc gcgcatcgca gcgaagatat cctcaccgat cataagctgc
      601 gggcgctgat ccaggcggcg gccggtctca aatttaacga tcccgccgaa agctacacaa
      661 agccggaaat cctgtcgttg ctcacgcaag cgcatgcgca acgcgaagtg aggatctatc
      721 aagcgggcag cggcattccc ggtgcgctct ggaccgtgct gatcggattc gaggccatcc
      781 tagttacttt cgttgttctg tccgggctgc gtttttggac gacggcagcg ctcgtctcag
      841 gcatattcgc cgcttcggtg tcgagcattc tggtcgtggc ccggctgctc gattatccgt
      901 tcgaaggcgc tctcgccctg gggccgcatg atttcatcgc ggtgacaaag aaggtgaccg
      961 aactgctcgt cagcggcggg tgacgctccc cgctggccga ttttgccggc tcccggagcg
     1021 aaacaataat atttacggca aaacagattg cactatacga tcgaatcctc ggcggattga
     1081 ctgtttgccg gagagtgaat ttgcatgagc gcgccggttt ttattacgca ttcctcgcgc
     1141 gattataagc aatcgcgggc gatcgtcgac gttctcgaga aaaacggcat cccctgctgg
     1201 atttccgagc gcgacatcgg cgcgggcgat aattacggcg acgccatcgt cgatgcgatc
     1261 gagaatgcca aggcgatggt gctggtcttc tccgtcaacg ccaacaattc cgacgagatc
     1321 aagaaggaaa tcgcgctggc gagccagcgc aaactgacgg tcgtgccgat ccgtatcgag
     1381 gatgcgacgc cgagcaaagc cttccgctat gaattgatca cccgcaactg gatcgacgct
     1441 ttccaagatt ggaatcaggc gatggagacg ctcacccggc gcgtctccgt catcgtcaac
     1501 ggggcggaag cccgtttgcc ggagcctgcg ccgaagccca aacccacgcc cggcatatcg
     1561 ccagccatga tcgcggcagt ggtcgccggg gtggcaatcg tcggcggcgg tgcggcttat
     1621 gggctttggc cacgaccgcc atcgccgatc atcattgtgc ataacgacga tacaattcat
     1681 cagaagccgg cgcccgcgcc tcaaccgccg ccggcttcgc cgaccgaacc caccgcggat
     1741 aagacggccg tcgtcgtgca aggcggcggt gaaaccgccc cggccgtcaa accggcggcc
     1801 ctgaatcccg ctccggcagc aaagctggcg tcgccatcgc ctgccgcgaa aaagaccgtg
     1861 gttgcggcgc tcattccgat ggttcccgcc gagaaaatcg cgccgcccgc ggccaagccg
     1921 gccgcaccgg cgatcgcgcc cgcgccaccg ccctataatc tcgcgacaaa cggcggccaa
     1981 gtctttcagg agtgcaagaa ttgtccgcaa atggtcgtcg tgccgcgcgg cgcagccacc
     2041 ttgggctcac ctgcgggcga gcccggccgg caatcgaacg aattgacgcc gcatggcgtg
     2101 acgatcggcg cgccgtttgc ggtgggccgc tatgcggtca ccttcgatga atgggacgcc
     2161 tgcgtcgccg acggcggctg caatggctac aagccggacg acaacggctt tggtcgcggc
     2221 aaacgcccgg tcatcttcgt ttcgtggaac gatgccaatg cctatgtcaa atggctcaaa
     2281 agcaaaaccg gagcgctcta tcgcctgctg agcgaggcag aatgggaata tgcggcgcgc
     2341 ggctgcacca cggccgaatg ccccaatacg cccttctggt tcggcgatat ctcgcccgag
     2401 cttgccaatt acgattcgcg ctattcctac gaggggagta cgaaagccga tcgcgcgttg
     2461 gagacggttc cggttgatca gggcaagccc aatccatttg ggctcaccaa tatacttgga
     2521 aacgtccagc aatgggtcga agactgttgg aacgcccaat tggatgcggt cccggcagac
     2581 ggcggccccg tgctcaccgg cgattgcagc gaccgggtga tccgcggcgg ttcctactcg
     2641 gacaagccgg atgccttgcg ggcggcatcg cgggcatggg atgcggcgga cgatcgtgaa
     2701 tcgccgaatg ttggatttcg tgtcgggcgc agcctgacgc cataagaaga ctgatacaaa
     2761 ctggacgtgc aattcgacgg gcggcgatag agaggttcgt gatgttgcgt ttgcgaatgc
     2821 accaggctgt ccgtgccctg gcatgtttgg gatttgccgg cctctgcgtc tttggcgcgg
     2881 tgagcggggc gcaggcggag acgcggcgtg ccttcgttct cggtgtgcag cgctattccg
     2941 actccgacat tcaaagcctc acccgttcgg atgccgatgc agccgacatc gccggcgatc
     3001 tggaagatct cggcttcgac aagaagaacg tcacgctcgc caccgatctg cgaacgaagg
     3061 acgacttcga caaaaagttc caggctttcc tcaagacgat tcaagaaggc gacgatgtcg
     3121 tcttcttttt ttccggccac ggcgtcggcg tcgaggcgac caatacgaat tatctgctgt
     3181 tcagcgattt gaagagcctc aagacctaca ccaagtctca attgctcgaa tcggatcggc
     3241 gcgacgatat catcgcgttg aaaatgccat ccttccaggg ccaatacgaa acggaagaaa
     3301 tccccaagga cggcgtttcg gcgtccgaaa tcatccagca gatcgcggaa aagaaaccgc
     3361 gcgtcgcttt catcgtcctc gacgcctgcc gctc
//
LOCUS       DASWOG010000364         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_265462,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000364 DASWOG010000000
VERSION     DASWOG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265462"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGB93_07615"
     CDS             <1..280
                     /locus_tag="VGB93_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0146005.1"
                     /translation="ACLDLLLDGGLLILIHRAEALPAILASLDARAAVTLMSVQPRKE
                     SAAKRILVRAQKGGRAPLTIAPALILHEGERFTAEADALHRGAALIDW"
     gene            complement(321..536)
                     /locus_tag="VGB93_07620"
     CDS             complement(321..536)
                     /locus_tag="VGB93_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146006.1"
                     /translation="MMQVKRKLALFAFASGLALAAASAEAAPAAGISQAAPAVSASEN
                     TTAPMVQKTWWHGHWHHWHHWHHWHHW"
     gene            664..1641
                     /locus_tag="VGB93_07625"
     CDS             664..1641
                     /locus_tag="VGB93_07625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY0146007.1"
                     /translation="MPTDTNLETQGLDPTRSFQGLILRLQTFWAAQGCAILQPYDIEV
                     GAGTFHPATILRALGPLPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVILKPSPDN
                     LQELYLGSLKAIGIDVSLHDIRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQV
                     AGIECAPVAGELTYGLERLAMYVQGVENVYDLNFNGRDGAEKLTYGDVFKQAEQEYSR
                     YNFEYADTDMLFRHFADAEAECKALLEKGEHGECHLMVLPAYDQCIKASHVFNLLDAR
                     GVISVTERQSYILRVRELAKACGKAWLKTKAGGADLAGSSVPTESESRL"
     gene            complement(1698..2585)
                     /locus_tag="VGB93_07630"
     CDS             complement(1698..2585)
                     /locus_tag="VGB93_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0146008.1"
                     /translation="MQALFIGHTYIDVTLLCDSIPYGDNKTVAQDYAVSFGGNAVTAA
                     FACAKLGHMPDLIANVADDWLGRMFLDMARAYQIRVHPRPVERSSLSFVLPNNGKRSI
                     IRARDDKFFHPFPNLDLDGCRVLHLDGHQGDAALHYAKACRARGILTSLDGGGLRGNL
                     DALLDLIDVAIVAERLCEQMRLSPQQMLEYLKGKGCKIGGVTQGERGLYWYNERGDVS
                     LLPALPVPKEKIIDTSGAGDIFHGAYVASYLAAPEKGFATHFRFGRAAAAYKITRLGN
                     EAGLPTQADVAAIIQEYAA"
     gene            complement(2660..3007)
                     /locus_tag="VGB93_07635"
     CDS             complement(2660..3007)
                     /locus_tag="VGB93_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0146009.1"
                     /translation="MNDIAVATPVNLTGNAVRHIAEILKTEPAGSALRIAVEGGGCSG
                     FQYKYAVVSATADGDLVLAEDGAKVAIDPISLDYLRGSRIDFVDELMGRSFKIDNPAA
                     TASCGCGTSFSLD"
     gene            3204..>3425
                     /locus_tag="VGB93_07640"
     CDS             3204..>3425
                     /locus_tag="VGB93_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175639.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0146010.1"
                     /translation="MPWRAPYASDASRSRGRLFPEANSPTRSEFQRDRDRILHSTAFR
                     RLSHKTQVFIPHEGDHYRTRLTHSIELGQI"
BASE COUNT          689 a         1008 c         1048 g          680 t
ORIGIN      
        1 cgcttgtctt gatttattgc tcgacggcgg tcttttgatc ctgatccatc gggcggaggc
       61 actacccgcg atcctggcgt cgctcgacgc gcgtgcggcg gtgacgctga tgtctgttca
      121 acctcgtaag gaaagtgcag ctaaacggat cctcgtccgc gcccaaaaag gcggccgcgc
      181 accgttgacg attgccccgg cgcttattct gcatgaaggc gagagattta cggcagaggc
      241 cgatgccctg catcgcggcg ccgctttgat cgattggtag attgcggcgc gagctttccg
      301 tcccgcgccg cctagatatc ttaccaatgg tgccaatggt gccaatgatg ccaatgatgc
      361 cagtggccgt gccaccaggt tttctgaacc attggtgccg tcgtgttctc gctcgccgaa
      421 acggcaggcg cggcctgact gatgccggcc gccggcgcgg cctcagcgct cgccgcagca
      481 agggcaaggc cggacgcaaa ggcaaacagg gcgagtttac gtttcacctg catcaatatt
      541 tctccctctt gatggatggc taaagccaaa aagatgcttg aacagaactg cgcaatcggg
      601 gacaggttcc cggacgacgc gagtccagcg ctcgcacttt catttgattg caaagatcag
      661 atcatgccaa ccgacacgaa tcttgagacg caggggctcg atccgacacg atcgtttcag
      721 ggactcattt tgcggctgca aaccttctgg gccgcacagg gctgcgcgat cctgcagccc
      781 tatgacatcg aggtcggcgc cggtacgttt catcctgcca caatattgcg ggcgcttggg
      841 ccgctgccct ggcgcgcggc ctatgtgcag ccgtcccgcc gcccgaagga cggccgctat
      901 ggcgacaatc cgaaccggct gcagcattat tatcaattcc aagtgatcct gaagccctcc
      961 ccggataatc tgcaggagct ttatctcggt tcgctcaagg cgatcggcat cgacgtctcg
     1021 ctgcacgaca tccgcttcgt cgaggacgac tgggaaagcc cgacgcttgg cgcctggggc
     1081 ctcggctggg aatgctggtg cgatgggatg gaagtctcgc aattcaccta tttccagcag
     1141 gtcgcgggca tcgaatgcgc gccggtcgcg ggtgaattga cctatgggct cgagcgtctt
     1201 gcgatgtatg tgcagggcgt cgagaatgtt tacgacctca atttcaatgg ccgcgacggc
     1261 gcggagaaat tgacttatgg cgacgtgttc aaacaggccg agcaggaata ttcacgctac
     1321 aatttcgaat atgccgatac ggatatgctg tttcgtcatt tcgccgacgc cgaagcggag
     1381 tgcaaggcgc tcctggaaaa gggtgagcat ggcgaatgtc atctcatggt actgccagcc
     1441 tatgatcaat gtatcaaggc ttcgcacgtt ttcaatctgc tggatgcgcg cggtgtgatc
     1501 tcggtgaccg aacgccagag ctatattttg cgcgtgcgcg agctggcgaa agcctgcggc
     1561 aaggcctggc tgaagaccaa ggccggcggc gccgatctgg ccggaagctc ggttccgacg
     1621 gagtcagaat cgagactcta ggatttttgt ttgacgcgtt ttcttcacgc gaaccgaaac
     1681 cacttcgctc gaaaacgcta tgcagcatat tcctgtataa tcgcagcgac atcggcctgc
     1741 gtcggcaatc cggcttcgtt gccaagacgt gtgatcttgt aggccgcggc ggcacggccg
     1801 aaacggaaat gcgttgcgaa gcctttttcc ggtgcggcga gatatgacgc gacataggcg
     1861 ccgtgaaaaa tatcgccggc gcccgacgta tcgatgatct tctctttcgg gacgggcagg
     1921 gcgggcagga gcgaaacgtc gccgcgttca ttgtaccagt agaggccgcg ctcgccttgc
     1981 gtgacgccgc cgatcttgca gcccttgccc ttcagatatt cgagcatttg ctgcggcgaa
     2041 agtctcatct gctcgcagag cctttcggcg acgatggcga catcgatgag atcgagcagc
     2101 gcgtcgagat tgccgcgcag gccgccgcca tcaagcgagg tcaggatgcc gcgcgcgcgg
     2161 caggctttcg catagtgcag cgcggcgtcg ccctgatgcc catcgagatg cagcacgcgg
     2221 cagccgtcga gatcgagatt aggaaaggga tggaagaatt tgtcgtcgcg ggcgcggatg
     2281 atggagcgct tgccgttatt cggcaaaacg aaagagagcg atgagcgttc gacggggcgg
     2341 ggatgcacgc ggatctggta ggcccgcgcc atgtcgagga acatgcgccc gagccaatcg
     2401 tcggcgacat ttgcgatgag atcgggcata tggccgagct tggcgcaggc gaaggccgcc
     2461 gtgacggcat tcccgccgaa ggagacggca taatcctgcg cgaccgtctt gttgtcaccg
     2521 taaggaatgg aatcgcagag cagagttaca tcgatatagg tgtggccgat gaacaaagcc
     2581 tgcacgcgtc tctcctcgtc cgagccaaga aaacgacgca ggacggtcga aagttcacgg
     2641 ccttaatgcc gtgcccgcgc taatcgagcg aaaagctggt gccgcagccg caagaagctg
     2701 tcgcggccgg attatcgatc ttgaaggagc gacccatcag ctcatcgacg aaatcgatcc
     2761 tgctgccacg cagataatcc agcgaaattg ggtcgatcgc gactttggcg ccatcctcag
     2821 cgagcacgag atcgccgtca gccgtcgccg atacgaccgc gtatttgtac tggaacccgg
     2881 agcagccgcc accctcgacg gcaatgcgca gcgccgaacc tgccggctcg gtcttcagga
     2941 tttcggcgat gtggcgaacg gcgttgccgg tgagattcac cggtgttgcg acggcaatat
     3001 cgttcatctt gacctcagat cgacagcgct gggcggtccg tcgtatcgct tgcatagggt
     3061 tgcgatagga agatatgtcg caaccgcgcc atctgcaacg aggcgggggc cgccgccgtg
     3121 ccctgcgaca gtttggcaaa aggaacccgg ctttgccgcc acactccgaa tccgcccctg
     3181 acggagggcc gtcgaccgcc ttcatgccgt ggcgcgcgcc ctacgcctcg gacgccagcc
     3241 gaagccgggg ccggcttttt cccgaggcaa attccccgac acggagcgaa ttccagcgtg
     3301 accgtgaccg tatcctgcat tcgacggcct tccgccgtct ctcccataag acgcaggtgt
     3361 tcattccgca cgagggcgat cattaccgca cacgactcac ccattcgatc gagcttggcc
     3421 agatt
//
LOCUS       DASWOG010000365         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_356453,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000365 DASWOG010000000
VERSION     DASWOG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356453"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VGB93_07645"
     CDS             complement(<1..1488)
                     /locus_tag="VGB93_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0146011.1"
                     /translation="MNIVRLALHRPYTFLVLGIAILLLGLSAILRTPTDIFPNINIPV
                     VTVVWYYDGLNNNETEKRITTYSEFSISFFVNDIRTIESQTIPGLVVEKIYFQPDVNV
                     DLAMSQVVSATNSIRAFLPPGAQPPIIMQYSASTVPVLQLALSSDRLSESELYDYGVY
                     RIRQQLAPIPGTMLPPPYGGRIRQVMVDIDPAALAAKGITPLDVSNAINAQNLSLPMG
                     DVKFGDKDYDVRINSLADAVSILDDVPIKKVNGALIRIKDVAEVRDGYAIQQNIVRSE
                     GRRSVLLSILKAGNASTLDVVDRVKNDVLPVSRAAAPPGMKIEELFDQSIFVRSAITG
                     VAVEATIAALLTSAMILLFLSSWRSTLIVAVSIPLSILSSIAILSAIGETLNIMTLGG
                     LALAVGILVDDATVTIENIHRLRQLGEKLPEATLHGAAGIALPTLVSTLAISAVFVSV
                     EFLVGPSKFLFTPMALAVVFAMLASYILSRTLVPIMAGLLLSGEHH"
     gene            1709..2296
                     /locus_tag="VGB93_07650"
     CDS             1709..2296
                     /locus_tag="VGB93_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0146012.1"
                     /translation="MALGRPRAFDADEALGKALKVFQRKGYEGSSIADLTEAMGINPP
                     SLYAAFGSKEELFRRAVDRYVGIREDFSHEALNAPTAREVVERLLRGTADQLTDPQSP
                     PGCLLVQGALSCGDAAEPVKKELCFRRVAEEVALRHRLERALEDGDLPKRLDVAGLAR
                     YIATVSQGMAVQAAGTATRADLHTVVNAVMQTWPS"
     gene            complement(2316..3587)
                     /gene="oxlT"
                     /locus_tag="VGB93_07655"
     CDS             complement(2316..3587)
                     /gene="oxlT"
                     /locus_tag="VGB93_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY0146013.1"
                     /translation="MTSQTDSFSGTTASAVTGRWIQLALGIICMMTISSPQYVWTLLT
                     KPLMAKLGIMLPSVQVTFTLLVLLQAFFSPFQGALIDRFGPRLLISIGTLLAGLSWVL
                     ASYAHSTLLLYLTYGCLGGLGTGIVYVGVVGLMVRWFPDRRGFAAGAVAAGYGMGAIV
                     STFPISASLAAHGLDATLRLYGTIFAVVGFAAAQGLRAPQFDTTANADLLALGGPRDM
                     RPADMLKTPLFWLMFAMMTMMATSGLMVTSQMAIFAADFGVAKVLVFGLAAVPLALTI
                     DRFANGLTRPLFGWVSDRLGRENTMFIAFALEGAAMTLWLLTRHDAVLFVLLSGLVFF
                     GWGEIFSLFPSTLTDTFGTRHATTNYGWLYISFGIGSIFGGPLAALLHQHAKSWIPVF
                     GCAIALDFCTALLALLALKPARARFLARRIG"
     gene            complement(3652..>4157)
                     /locus_tag="VGB93_07660"
     CDS             complement(3652..>4157)
                     /locus_tag="VGB93_07660"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0146014.1"
                     /translation="YHEFGSGIVLPNSGINWQNRGCSFSLDPNVRNALAPGRRPFHTL
                     NPPLARFNDGRTIVYGAMGGDGQPQSQSAVFSRIAKFGWDPQSAVSAPRWLLGRTWGR
                     ASDTLKLEARFPDATFEALRARGHEVESMSAYDEAMGHAGAIIRYPDGTFEGGYDPRS
                     DGAAAGW"
BASE COUNT          938 a         1206 c         1314 g          699 t
ORIGIN      
        1 atgatgttcg ccagacagca gcaagccggc catgatcggc acgagcgttc gcgacaggat
       61 gtacgaggca agcatcgcga agacgaccgc gagcgccatg ggcgtgaaga ggaatttgga
      121 cgggccgacg agaaattcga ccgacacaaa aacggccgag atcgccagcg tcgaaacaag
      181 ggtcggcagc gcgatacctg cggcgccatg cagcgtcgcc tccggcagct tttcgccaag
      241 ctggcgcagc cggtgaatgt tctcgatcgt caccgtcgca tcgtcaacga gaatgccgac
      301 ggcaagagca agaccgccga gcgtcatgat gttcagcgtc tcgccaatgg ccgagagaat
      361 ggcaatcgat gacaggatcg aaagaggaat cgagacggcg acaatgagcg tcgagcgcca
      421 gctcgagaga aacagcagaa tcatcgccga agtgaggagc gcagcgatcg ttgcctcgac
      481 ggcaacaccg gtgatcgccg agcgcacgaa aatcgattga tcgaacaatt cctcgatctt
      541 catgccgggc ggcgcggcgg cgcggctgac cggcagaacg tcattcttca cccggtcgac
      601 gacgtcgagc gtcgaggcat taccggcttt caggatggag agaagaacgg agcggcggcc
      661 ttcgctacgc acgatattct gctggatggc atagccgtcg cggacctcag ccacatcctt
      721 gatgcggatc agcgcgccat tgaccttctt gatcggcacg tcatcaagaa tggaaacggc
      781 gtcggcgaga ctgttgatgc ggacatcgta atccttgtcc ccgaatttca cgtcgcccat
      841 cggcaacgag agattctgcg cattgatcgc gttggagacg tccaacggcg tgatgccctt
      901 ggccgccagc gcggcggggt cgatatcgac catcacctgg cggatgcgcc cgccataggg
      961 cggcggcagc atcgtgccgg ggattggcgc aagctgttgg cggatgcgat agacgccgta
     1021 atcgtaaagc tcggattccg agagccggtc ggaactcagc gccaattgca agacgggcac
     1081 ggtcgaggcg ctatattgca tgatgatcgg cggctgagcg ccggggggca gaaaggcgcg
     1141 gatcgaattc gtcgccgaga cgacctggct catcgccaga tcgacattca catccggctg
     1201 gaaatagatc ttctcgacga cgaggccggg aatcgtctgg ctctcgatcg tgcggatgtc
     1261 gttgacgaag aagctgatcg agaattccga ataggtcgtg atccgctttt cggtctcgtt
     1321 attgttcagg ccgtcgtagt accagacgac cgtcacgacc gggatgttga tgttcggaaa
     1381 aatatcggtc ggcgtccgca gaatggccga gaggccgaga agcaggatcg caatgcccag
     1441 gacaaggaac gtgtacgggc gatgcagggc caggcggacg atgttcataa agcaaattcc
     1501 actttccgcc cggagacgcc ccgaagcggc gcctgacggc ggcgatcaca attatgtagg
     1561 gaaccctaca attatatgct gcattgcgcc atatcaagtt ccggtgtttc tcccagcgat
     1621 atgagaaggg cgcgtgacgt ttgccgccta gacagggcgc accaccggca ttatttatcg
     1681 atcaataaaa aaggtcaacg aggccgacgt ggcattagga cgtcctaggg cttttgatgc
     1741 cgacgaagcg ctcgggaagg cactcaaggt cttccagcgc aagggttatg agggttcgtc
     1801 gattgcggac ctcaccgagg ctatgggcat caatccgcca agtctctacg cagctttcgg
     1861 cagcaaggaa gagttgttcc gtcgcgcggt cgaccgttat gtgggcattc gcgaagactt
     1921 ttcgcatgaa gccctgaatg cgccgacggc gcgcgaggtc gtcgagcgct tattgcgcgg
     1981 gaccgccgac cagctcaccg atccgcaaag cccccccggc tgtctgcttg tgcagggcgc
     2041 cctctcctgc ggcgacgcgg ccgagcccgt aaaaaaagag ctttgcttcc gtcgcgtggc
     2101 tgaggaagtc gcgctgcgtc atagactcga gcgggcgctg gaggacggcg atttgccgaa
     2161 acgcttggat gtcgccggat tggcgcgtta catcgccacc gtgagccagg gcatggcggt
     2221 gcaagccgcg ggcaccgcga cgcgcgccga cttgcacact gtcgtcaatg cggtgatgca
     2281 aacctggccg agctagagcc gcaggaactg cgcagctaac caatgcgccg cgcgagaaaa
     2341 cgggccctag ccggcttcaa cgcgagaagt gcgagaagtg cggtgcagaa atcgagcgca
     2401 atcgcgcagc cgaaaacagg aatccagctt ttggcgtgct gatgcagcag ggcggccagt
     2461 gggccgccga agatcgagcc aatcccgaac gagatataaa gccagccgta gtttgtggtc
     2521 gcgtgacgcg tgccgaacgt gtccgtcaat gttgacggaa acagggagaa aatttcgccc
     2581 cagccgaaga agacaagccc agacagcagc acgaacaaaa ccgcgtcgtg acgcgtgagg
     2641 agccaaagcg tcatcgccgc gccttcgagt gcgaaggcga tgaacattgt gttctcgcgg
     2701 ccgagacgat ccgaaaccca gccgaacaac ggccgtgtca gaccgttggc gaaccggtca
     2761 atagtcaacg ccagcggtac tgcggcaaga ccgaagacga gcactttggc gacaccgaag
     2821 tccgcggcga aaatcgccat ctgcgacgtg accatcaggc cggaggtcgc catcatcgtc
     2881 atcatggcga acatcagcca gaagagcggc gtcttgagca tgtcggcggg gcgcatgtcg
     2941 cgcggcccac cgagcgcgag caagtccgcg ttggcggtcg tgtcgaattg cggcgcgcgt
     3001 aaaccttgtg cggcggcaaa gccaacaacg gcgaaaatcg ttccgtaaag ccgcaacgtc
     3061 gcatcaagcc cgtgcgctgc gagtgacgcg gaaatcggaa aggtcgagac gatcgcgccc
     3121 atgccgtagc cggctgcgac cgcgccagcg gcaaagccgc gccgatccgg aaaccagcgc
     3181 accatcagcc cgacgacgcc cacatagacg atgccggtgc cgaggccgcc caggcaaccg
     3241 taggtcaggt aaagcagcag cgtgctatgc gcgtaagatg cgagaaccca gctcagaccc
     3301 gcaagcaacg tcccgatcga gatcagcaga cgcggcccga agcggtcgat caacgcgccc
     3361 tggaatggcg agaaaaacgc ctgcaacagc acgagcaacg tgaacgttac ctgcacactc
     3421 ggcaacatga taccgagctt cgccatcaaa ggtttggtca gtagcgtcca gacatattgc
     3481 gggctcgaaa tcgtcatcat gcagatgatg ccgagggcaa gttgaatcca tcggcctgtg
     3541 accgcggatg ccgtggttcc gctaaacgaa tctgtctgag atgtcataat tgtttcctga
     3601 tcgcatcaac gtgggcgatc tgtgcgcgat ccggaatcca atgcatcatt cttaccaacc
     3661 ggcggcagcg ccgtcgctgc gcggatcgta accaccttca aatgtgccat ccggatagcg
     3721 gatgattgcg ccggcgtggc ccatggcttc gtcataggcc gacatgctct cgacttcgtg
     3781 accgcgggcg cgcaaagcct caaaggtcgc gtccgggaaa cgtgcctcaa gtttgagcgt
     3841 atcgcttgcc cggccccaag tgcggccgag cagccaacgc ggcgcgctga cggccgattg
     3901 tgggtcccag ccaaatttgg cgatgcgcga gaagacggcg ctttgcgatt gcggctggcc
     3961 gtcgccaccc atcgcgccat aaacgatcgt gcggccgtcg ttaaagcgcg ccagcggcgg
     4021 gttcaatgta tggaaggggc ggcggcccgg cgcgagggcg ttgcggacat tggggtcgag
     4081 cgaaaagctg caaccgcgat tctgccaatt gattccggag ttcggcaaca cgatgccgct
     4141 accgaattca tgataga
//
LOCUS       DASWOG010000366         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_136991,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000366 DASWOG010000000
VERSION     DASWOG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136991"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..3718)
                     /gene="metH"
                     /locus_tag="VGB93_07665"
     CDS             complement(2..3718)
                     /gene="metH"
                     /locus_tag="VGB93_07665"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0146015.1"
                     /translation="MSKVPASDCHNELRAAAAERILVLDGAMGTMIQTHKFSEADFRG
                     ERFADWPRDLRGNNDLLVLTQPHAIEAIHFAYAQAGADIIATNTFSSTRIAQADYGME
                     DLVPELNREAARLCRNAADRAQAKDGRRRFVAGSIGPTNRTASISPDVNNPGFRAVSF
                     DELRTAYAEAAEALIEGGSDVLLIETIFDTLNAKAAYAGIDDVFEKIGRKLPVMISGT
                     ITDLSGRTLSGQTPTAFWYSLQHTAPLSIGLNCALGAREMRAHLAELSRIADTLICAY
                     PNAGLPNEFGLYDESPEYMAELIGEFADSGLVNIVGGCCGTTPSHIHAMAKRVAGLKP
                     REIPEIEPRLRLSGLEPFTLTPDIRFVNVGERTNVTGSARFRKLIKEGDYAAALDVAR
                     DQVSNGAQIIDINMDEGLLDSQQAMVDYLNLIAAEPDIARVPVMIDSSKFSVIEAGLK
                     CVQGKSIVNSISLKEGEESFIEHAKLVYRYGAAVVVMAFDEKGQADTFGRKVEISARA
                     YKILTETVGFPPQDIIFDPNIFAVATGIEEHNNYGVDFIEATRKIRERFPLVHISGGV
                     SNLSFSFRGNERVREAMHSVFLYHAISAGMDMGIVNAGQLAVYADIEPELREACEDVV
                     LNRRPDSTERLLALAESYKGKGMEAKEKDLAWREMPVEKRLEHALVAGITEYIDIDVE
                     EARQMSTRTLDVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPYMEA
                     EKSGARSTAGKILMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPAQKILDTAKA
                     ENVDAIGLSGLITPSLDEMCFVASEMEREGFDLPLLIGGATTSRVHTAVKIHPNYHRG
                     QAVYVTDASRAVGTVQSLLSHDNRVAYIDKVRSEYQKVAEAHARGERDKARISLVQAR
                     ANGLKLDFAAYTPPKPTFTGTRVFRSYDIEELIPYIDWTPFFRTWELRGRYPEIFDDK
                     EQGEAARQLYDDAQEMLKQIVAERWFDPKPIIGFWPANAVGDDIRLYTGESRGDTLAT
                     LHTLRQQLSKREGRRNVALADFIAPEESGKADYIGAFVVTAGAREEKLAERFAKANDD
                     YRSILVKALGDRIAEALAERMHERVRHEFWAYAPDEALSNDDRLTEKYRGIRPAPGYP
                     AQPDHTEKGTLFKLLLAERRIGVKLTESFAMWPGSSVSGLYLAHPEAHYFGVGKIERD
                     QVEDYAKRKGMSVDEAERWLAPVLNYDRTPVAAE"
     gene            complement(3718..>4262)
                     /locus_tag="VGB93_07670"
     CDS             complement(3718..>4262)
                     /locus_tag="VGB93_07670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY0146016.1"
                     /translation="FRPHPEGYANSTALVAGLKSLADFEITVSAYPEGHPESASFEQD
                     IDVLKAKIDAGATRAITQFFFENEVYFRFLDKAYDRGITIPIIPGIVPVQNFRQTARF
                     AQRAGATVPTWLAERFEGLDDDPTTRRLIAATVAAEQVFDLVDAGVEHFHFYTMNRAD
                     LVYAICHLLGLRPQAETRAA"
BASE COUNT          824 a         1240 c         1324 g          874 t
ORIGIN      
        1 gctattccgc cgcgacgggc gtgcggtcgt agttgagaac cggcgccagc cagcgctccg
       61 cttcgtcgac ggacatgccc ttgcgtttcg cataatcctc gacctgatcg cgctcgatct
      121 tgccgacgcc gaaataatgc gcttcgggat gcgccagata gagcccgctg acggacgagc
      181 ccggccacat cgcgaaactc tcggtcagct tcacgccaat acgtcgttcc gccagcagaa
      241 gtttgaacag cgttcccttt tcagtgtgat cgggctgcgc gggatagccg ggcgccggcc
      301 ggatgccgcg atatttttcc gtaagccggt cgtcgttcga cagcgcctca tccggcgcat
      361 aggcccagaa ttcgtgccgc acgcgctcgt gcatccgctc ggccagagcc tcggcaatgc
      421 ggtcgcccag cgctttgacg aggatggaac gataatcgtc gttggccttg gcgaaacgct
      481 cggcaagctt ttcttcgcgc gccccggcgg tgacgacgaa agcgccgata taatcggctt
      541 tgccgctctc ttccggggcg atgaaatccg cgagcgcgac attgcggcgt ccctcccgct
      601 tcgagagttg ctggcgcagt gtatgcaacg tcgctagcgt atcgccgcgt gattcaccag
      661 tgtagagcct gatatcatcg ccgaccgcat tcgcgggcca gaagccaatg atgggctttg
      721 ggtcgaacca gcgttcggcg acgatctgtt tcagcatttc ttgcgcgtcg tcgtagagct
      781 ggcgcgccgc ttcgccttgt tccttgtcgt cgaagatttc ggggtaacgg ccgcgcagct
      841 cccaggtgcg gaagaacgga gtccaatcga tataagggat caattcttca atatcgtaag
      901 agcggaaaac gcgtgtgccg gtgaacgtcg gctttggcgg cgtataggcc gcgaaatcga
      961 gcttcaatcc gttggcacga gcctgcacga gcgaaatgcg cgccttgtcg cgttcaccgc
     1021 gcgcatgggc ttcggcaacc ttctgatatt cgctgcgcac cttgtcgata taagcgacgc
     1081 ggttatcgtg cgagagcagc gattgcacgg tgccgaccgc gcgactcgcg tcggtgacat
     1141 agacggcctg gccgcgatgg taattcggat gaattttcac cgcggtatgg acacggctcg
     1201 tcgtcgcccc accgatcagg agcggcaggt caaagccttc gcgttccatt tccgacgcga
     1261 cgaagcacat ttcatcgagc gatggtgtga tgaggcccga aaggccgatg gcatcgacat
     1321 tctcggcctt cgccgtatcg agaatcttct gcgccggcac catgacgccg agatcgatga
     1381 tctcgtaatt gttgcaggcc agaacgactc cgacaatgtt tttgccaata tcatgcacgt
     1441 cgcccttgac ggtcgccatc agaatcttgc cggcggtcga gcgcgcgcca gatttctccg
     1501 cctccatata gggcaggaga taggcgacgg cttttttcat cacgcgggcg gatttgacga
     1561 cctgcggcag gaacatcttt cccgagccga agagatcgcc gacgacattc atgccggcca
     1621 tcagcgggcc ttcgatgacg tcgagcgtcc gcgttgacat ttgccgcgct tcttcgacat
     1681 caatgtcgat atattcggtg atgcccgcga cgagtgcgtg ttccagccgc ttctcgacag
     1741 gcatctcgcg ccaggcgagg tctttctcct ttgcctccat gcccttgccc ttgtagcttt
     1801 cggcaagcgc aagcaggcgc tcggtcgaat ccgggcgtcg gttcagcacg acatcttcgc
     1861 aggcttcgcg aagttctggt tcgatatcgg cataaaccgc gagctggccg gcattaacaa
     1921 ttcccatatc catgcccgcg ctgatcgcgt ggtatagaaa gacagagtgc atcgcctcgc
     1981 gcacccgttc gttgccgcgg aacgaaaaag acaggttcga gacgccgccg gaaatatgga
     2041 cgagcgggaa acgttcgcga attttccgcg tcgcctcgat gaaatcgacg ccgtaattgt
     2101 tgtgctcttc gatgcccgta gcgaccgcga aaatgttcgg gtcgaagatg atatcctgcg
     2161 gcggaaaccc gaccgtctcc gtaaggattt tataggcacg cgcggaaatc tcgactttgc
     2221 gcccgaacgt atccgcctgg cccttctcat cgaacgccat gaccacgacg gcggccccat
     2281 aacggtagac gagcttggcg tgttcgatga aagattcctc gccttccttc aaggagattg
     2341 agttgacgat cgacttgccc tgaacgcatt tgaggccggc ctcgatcacc gagaatttcg
     2401 aagaatcgat catcaccggc acgcgggcga tgtccggctc cgcggcgatg agattgagat
     2461 aatcgaccat tgcctgttgc gaatcgagca ggccttcatc catgttgatg tcgatgatct
     2521 gcgcgccgtt gctgacctgg tcacgtgcga cgtcgagcgc cgcagcataa tcgccttcct
     2581 tgatcaattt gcggaagcgc gcggagccag tgacattggt gcgctcgcca acattgacga
     2641 agcggatgtc gggcgtcagc gtaaaaggtt cgaggccgga gagccgcagg cgcggctcaa
     2701 tctcgggaat ttcccgcggc ttcaggccgg cgacgcgctt ggccatggca tgaatatgcg
     2761 acggcgtcgt gccgcagcag ccgccgacga tattgacgag gcctgagtcg gcgaattcgc
     2821 cgatcagctc cgccatatat tcggggctct cgtcatagag tccgaattcg ttcggcaggc
     2881 cagcattggg ataggcgcag atcagcgtat cggcaatgcg cgaaagctcg gccagatggg
     2941 cgcgcatctc gcgtgcgccg agcgcgcaat taagcccaat cgacaagggc gccgtgtgct
     3001 gcagcgaata ccagaacgct gtcggcgtct ggccggaaag cgtgcggccg gacaaatcgg
     3061 tgatcgtgcc ggaaatcatc accggcaatt tgcggccgat cttctcgaac acatcatcga
     3121 tgccggcata agccgctttg gcattgagcg tgtcgaaaat cgtttcgatc agcaaaacat
     3181 cggagccgcc ttcgatgaga gcttcggcgg cttcggcata ggcggtgcgc agctcgtcga
     3241 aactgacggc gcgaaagccg ggattattga cgtcgggcga gatcgaggcg gtgcggttcg
     3301 tcgggccgat ggagccggcg acgaagcggc ggcggccatc cttcgcctgc gcgcgatcgg
     3361 cggcattgcg gcagaggcgt gccgcctcgc ggttcaattc cggcaccagg tcttccatgc
     3421 cgtaatcggc ctgggcgatg cgggtcgaag aaaaagtatt ggtcgcgatg atgtcagcgc
     3481 cggcttgcgc ataggcgaaa tggatcgcct cgatcgcgtg cggctgtgtc agcaccagca
     3541 gatcgttgtt gccgcgcagg tcgcgtggcc aatcggcgaa acgttcaccg cggaaatccg
     3601 cctcgctgaa tttatgcgtc tggatcatcg tgcccatggc gccgtcgagc acgaggatgc
     3661 gttctgcggc ggcggcgcga agttcgttat gacaatcgga cgcgggaact ttggacatta
     3721 agcagctctc gtctcggcct gggggcgcag gccgagcagg tggcagatcg catagacgag
     3781 atccgcgcgg ttcatcgtgt agaaatggaa atgctcgacg ccggcgtcaa cgaggtcgaa
     3841 gacctgttcg gcggcgacgg tcgcggcgat caaccgcctg gtcgtcggat cgtcgtcgag
     3901 accttcaaac cgttcggcga gccatgtcgg caccgtggcg ccggctcttt gtgcgaagcg
     3961 cgcggtttgg cggaagttct gcaccggaac gatgccgggg atgatcggaa tggtaatgcc
     4021 gcgatcgtag gccttatcga gaaagcggaa atagacctcg ttctcgaaga agaattgagt
     4081 gatggcgcgg gtcgcgccag catcgatctt ggctttcagg acatcgatgt cctgttcgaa
     4141 cgaggcgctt tccggatggc cttcgggata ggccgagacc gtgatctcga aatcagcgag
     4201 gctcttgagg ccagcgacca atgctgtcga attggcgtag ccttcgggat gtggcctgaa
     4261 gc
//
LOCUS       DASWOG010000367         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_209821,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000367 DASWOG010000000
VERSION     DASWOG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209821"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..503
                     /locus_tag="VGB93_07675"
                     /pseudo
     CDS             1..503
                     /locus_tag="VGB93_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
     gene            1504..>1938
                     /locus_tag="VGB93_07680"
     CDS             1504..>1938
                     /locus_tag="VGB93_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY0146017.1"
                     /translation="MDRFVSCFTNRLDAKGRVSIPAQFRAVLARDGFDGLFVHPALEG
                     EAVDCGGNALLREIDQLLARHAPYSEERDHLSTALFGASEILKVDAEGRVVLSETIRA
                     LTQVTQEVTFLGKGDRFQIWEPSRFQAHFEEAKARSRVLRRQS"
BASE COUNT          404 a          569 c          598 g          367 t
ORIGIN      
        1 tcgtcactgc gtcgggctgc ggcacgacca tcaaggatta cggtttcatg ctgcgcgaag
       61 atcccgcata tgccgacaag gcggcagaag tctcgcggct tgccaaagac atttgcgaat
      121 atgccgccgg gctcgatctc ggcgcgacgg cgcagcctca agagctgacc gtcgcctatc
      181 atgccgcctg ttcgctgcag cacggacaga aaatcaccga tcagccgaag cggctgatga
      241 aagccgcggg tttccgtctg cgcgaggtgt cggagggcca tatctgctgt ggttccgccg
      301 gcacctacaa catcatgcag ccctatttcg ccgagcagct tcgcgaacgt aaaatcggca
      361 atatcgaagc tgcgcggccg gacgtcatcg ctctcggtaa tatcggctgt atgatgcaga
      421 ttgcgagcgg cacggatatt cccgtggttc atacaatcga gttgctggat tgggcgaacg
      481 gcggtccaaa gcctttgcaa atcgaacacg ccggcgcaag atgtcactct cggcgggaga
      541 cggacatggc ggaggataag cgcaaacgaa aagcaccgct gaagaccgcg cgaaacgcgg
      601 cagtgaagaa gcgcgcgcca gccaagaaaa agccggcgtt gaaaaaatcc gtcgcgaaga
      661 aagtcgttgc caaaaaatct gtcgccaaat cgtctgctgc gaaatcgtct gctgcgaagt
      721 cgtctgtggc caagaaaatt ccggcgcgga aatccgtgcc gatcaaaaaa gcgcgggtaa
      781 gcaagcgcgc gtttctgccg cctttgccgc cgccagcctc ggcccgcaaa gccccacgca
      841 gcccggcgcg gtcaccatcg cgcgccaagg cgcttggccg caccaagacc ggcggccaca
      901 aagctgtcca tgacatcccg gcctgcacgg aagtcgtgtt gcggcagaag ctgcggctcg
      961 ccgcgcctta catcgagctt tggctcgtgc agaaggacgg tgcacgggcg gaagagggga
     1021 cgacgccgga agccgcgttc gttcgcatct ggcaatggct catgctggcg gcggcgctcg
     1081 aaggcagcca tgtcgtgatc acgcctgacc tcgtccaaaa gcttttcgct gaggaaatgg
     1141 gacgcgctcc caccgtctag gctcaaagcg ggcccggatc tcagccgcga ttgcttccat
     1201 cgcgggtctt acaaaaaata tctcacagcc aaatagcgac aggggcgcgg agattccgcg
     1261 ccaatgccgt ttttcaaccc gctgttaacc ttaacatttt ctaattcaga tgaatccttg
     1321 cgtcaccctg cgtccccacg ccggggcgcg ccaacccacg ttgaccggca ttatatccca
     1381 tgttatccca aatcatccca tggttgaggc tcgccgcacc acccggcgtt tgggtggtcg
     1441 gggaaggcga gccgaccggg aatgtttttg cggcgtggcg ttttgggtgg cggaggcggg
     1501 ggcttggacc ggttcgtctc gtgtttcacc aatcggctgg atgcgaaggg tcgcgtttcg
     1561 atcccggcgc aattccgggc ggttctggca cgcgatggat tcgacgggct cttcgtgcat
     1621 ccggctctcg agggcgaggc agtcgattgc ggcggcaatg ccttgttgcg ggaaatcgat
     1681 cagcttttgg cgcggcacgc gccgtattcg gaagaacggg atcatctctc gacggcgctt
     1741 ttcggcgcca gcgagattct gaaagtggat gcggaaggcc gcgtcgttct ctcggaaacg
     1801 atcagggctc tgacgcaggt tacgcaggaa gtcaccttcc tcggaaaagg agacaggttt
     1861 cagatctggg agccgagccg ttttcaggcg catttcgagg aggccaaagc tcgatcgcgc
     1921 gttcttcgaa gacaatca
//
LOCUS       DASWOG010000368         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_246888,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000368 DASWOG010000000
VERSION     DASWOG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246888"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB93_07685"
     CDS             <1..207
                     /locus_tag="VGB93_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333003.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0146018.1"
                     /translation="AGAVEAIFCTLAIRDQIVPPTLNLDNPSVTTSIDLVPHKARKRE
                     VEFVLSNSFGFGGTNASLILKAVA"
     gene            375..2066
                     /gene="mltG"
                     /locus_tag="VGB93_07690"
     CDS             375..2066
                     /gene="mltG"
                     /locus_tag="VGB93_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0146019.1"
                     /translation="MTRQGSDDGEPPARFFGRRPKLQSPNEALQPETPPPPPSPHKRR
                     PTLSAMSGFLSFLLLVAFGALFGVVWGAHRLQQPGPLPADKVLYIQPGTAADIVAQLD
                     EEGIVDSPLLFNIALVVEGDRSKMKAGEYLIHRNASMREVMDTLVSGRQILHPVTIPE
                     GLTSQQVVDKLRANDLLMGDIREIPPEGSLLPETYKIARGMPRADLIRLMQEDDRKLV
                     AEIWANRSPDLLLTSPYEMVTLASIVEKETGKADERPHVASVFLNRLHKHMRLQSDPT
                     IVYGIMAGRGSLGRPLTHADVEQSSPYNTYVIEGLPPGPIANPGRAALEAVANPSRTS
                     DLYFVADGTGGHVFASSLEEHNRNVARWRQIRKDAKDKAGPDSDVDKYVPPEHGQRSD
                     NDSSVYGALPTSFGAVDQSGLSSYASSLGMGATAAAIAQVAPALPHEAAAPATAADPV
                     VADAVSTQAADKPTPGLGPSLDDLGITVRGMDQPASTKKLLDGPVDSSASVAPADPQA
                     FADAAVGPAPNGREPPAHPHIIDASEGTPLDPLRNTTYDLNYAKTVPSASQLKQP"
     gene            2088..>2494
                     /locus_tag="VGB93_07695"
     CDS             2088..>2494
                     /locus_tag="VGB93_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0146020.1"
                     /translation="MTIASMTGFARVSGATANFRWAWELKSVNAKGLDLRLRLPPGFD
                     AIEIEARGLLSGKLTRGTCYATLAAQREAAMPEVHLNEPLLRRLVEAVSHIEHSDSLR
                     PASIDGLLGVRGVVEIRDTGDEETDLAQVRSGAL"
BASE COUNT          459 a          797 c          764 g          474 t
ORIGIN      
        1 gcgggtgcgg tcgaggcgat tttctgcact ttggcgatcc gcgatcaaat cgtgccgccg
       61 accctcaacc tcgacaatcc ttcggtaacg acctcgatcg acctcgtgcc gcataaggcg
      121 cgtaagcgcg aggtggaatt cgtgctctcg aactcctttg gcttcggcgg caccaatgcc
      181 agcttgatcc tgaaggcggt cgcgtaaagt ccaagccaaa tttcctccgc aaagaggtgg
      241 aattgtcccg gtttggcagg gcgcgtctcc agcgccggtt gtttgccgcc ttggccgcat
      301 ttcgtgtaag actgacgcgc ccggcgcatg gataaggttc gatggcaggc tcgccacaaa
      361 atgacggagg cgcattgacg cgtcagggat ccgacgacgg ggaaccgccg gcgcggttct
      421 ttggccgccg tccgaagctc caaagtccga acgaggcact gcagccggag acgccgccgc
      481 cgccgccatc gccgcataag cgccggccga cattgtccgc gatgtccggt ttcttgtcct
      541 ttcttctgct cgtcgccttc ggcgcgctgt ttggcgtcgt ttggggcgcg catcgcctgc
      601 aacagcccgg ccccttgccg gccgacaaag ttctttacat tcagccgggc acggccgccg
      661 acatcgtcgc ccaactcgat gaagagggga tcgtcgacag cccgctcttg ttcaacatcg
      721 cccttgtcgt cgaaggcgac cgctcgaaga tgaaggcggg cgaatatctc atccatcgca
      781 acgccagtat gcgcgaggtg atggatacgc tggtgtccgg ccgccaaatc ctccaccctg
      841 tgacaattcc cgaagggctg accagccagc aggtcgtcga caagctgcgc gccaacgatc
      901 tcctgatggg cgatattcgc gaaatcccgc cggaaggtag tcttctgccg gagacctaca
      961 agattgcgcg cggtatgccg cgtgccgatc tgatccggct gatgcaggag gacgacagaa
     1021 agctcgtcgc ggaaatctgg gcgaatcgtt cgccggatct gctgctgact tcgccgtatg
     1081 aaatggtgac gctcgcctcg atcgtcgaaa aggaaaccgg caaggccgat gagcggccgc
     1141 atgtcgcttc tgtcttcctc aatcgtctcc acaaacatat gcggctgcaa tccgatccga
     1201 caattgttta cggaatcatg gcgggccgtg gctcgctcgg ccggccgctg acccacgcgg
     1261 atgtcgagca gtcgtcgccc tataatacct acgtaatcga aggcctgccg ccagggccga
     1321 tcgccaatcc cggccgcgct gcgcttgagg ccgtcgccaa tccctcgcgc acgagcgatc
     1381 tttattttgt cgccgatggt acgggcggtc atgtctttgc ttcttcgctc gaagagcaca
     1441 accgcaacgt cgcgcgttgg cgccagatcc ggaaggacgc gaaagacaaa gccgggccgg
     1501 actcagatgt cgataaatat gtgccgcctg aacatgggca gcgcagcgat aacgactcgt
     1561 ctgtttatgg cgcgctgccg acatcgttcg gagcagtgga ccagagcggc ttgagttcct
     1621 atgcgtcgag cctcggcatg ggcgcgacag ccgccgcgat cgcgcaggtc gcgccggcct
     1681 tgccgcatga agcggctgct cccgcgacgg cggccgatcc cgtcgttgcc gacgccgttt
     1741 cgacgcaggc tgccgacaag ccaacgccgg gccttggccc gagcctcgac gatcttggca
     1801 tcaccgtgcg tggcatggat caaccggcga gcacgaaaaa actgctcgat ggtccggtcg
     1861 atagttccgc ctccgttgcg cccgccgatc cgcaagcctt cgccgatgcg gcggtcggac
     1921 cggcaccgaa cgggcgcgaa ccgccagccc atccgcacat tatcgatgcc tccgagggca
     1981 cgccgctcga tcctttgcgc aatacgacct acgatttgaa ttatgccaag acggtgccat
     2041 cggcttcgca actcaagcag ccgtaacctc agagaaaacg aactccattg actattgcca
     2101 gcatgaccgg cttcgcgcgg gtctccggcg ccaccgcgaa ttttcgttgg gcgtgggaat
     2161 tgaagtcggt caatgccaag ggcctcgacc ttcggttgcg gctgccgccc ggcttcgatg
     2221 cgatcgagat cgaagcgcgg ggcttgctgt cgggcaagct cacccgcggc acctgctatg
     2281 cgacgctagc ggcccaacgc gaggccgcga tgcccgaagt ccatctcaac gaaccgcttt
     2341 tgcggcggct ggtcgaggcg gtctcgcata ttgagcatag cgattcgctg cgtccggcat
     2401 cgatcgacgg tttgctcggt gtgcgtggcg tcgtcgaaat ccgcgatacg ggtgatgagg
     2461 agacggacct ggcacaggtc cgcagcgggg cgct
//
LOCUS       DASWOG010000369         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_155049,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000369 DASWOG010000000
VERSION     DASWOG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155049"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..789)
                     /locus_tag="VGB93_07700"
     CDS             complement(205..789)
                     /locus_tag="VGB93_07700"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEY0146021.1"
                     /translation="MDMQIRRVATIIEDHFYEAGRRAERPLRKVATIMVLKNPYAGHY
                     VEDLSALTEASIGLGKKMGAQAVAVLGGAPVEAYGKAGIVGLNGETEHANALLTTVFA
                     NPIRDAIGGGDAWISSVTKVAAPGTMIDVPMNSIDDVYIRSHYGTMSLVLPDAPLPDE
                     VALIFCFATGGRLNARVGGITVEEVRKQRATAKA"
     gene            1106..1795
                     /locus_tag="VGB93_07705"
     CDS             1106..1795
                     /locus_tag="VGB93_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0146022.1"
                     /translation="MDFKVLPQSVQGQAVSKLRDAIVTGIFRPGDRLVESDLCTRLGI
                     SRPSVREALRSLEAERLVVIVPNRGPMIPILTVDMARQIYDVRALLEGEAAALFAAHA
                     PPSAIAAMRSALEAFDAAVKSGDLTEEIRATRQFYEPMLLGCGNRVIFEVLDTLSARI
                     TFLRSKSMSLPGRAQKSAREMRAILKGITSGDAEAARTAAVTHVRQACNAAVKAYSAD
                     KIAEKTTKTSG"
BASE COUNT          380 a          559 c          511 g          377 t
ORIGIN      
        1 cgtcgtgcct ctcgtgcgtt cggtcaacga tcagcttttc gacagtgtga cacgcgacga
       61 attgcagtcg atcctgcaat tcttccggcg cttcgcgacc aatagtgaaa ttgccttgac
      121 caggatcaag tccgccaaga agcccgcgaa gcggcgaagc accacgacgc cggactgatc
      181 tcagtccggc ggcagatgtt ggaactaggc tttggccgtt gcccgttgct tccgcacttc
      241 ttccaccgtg ataccgccca cgcgagcatt cagccggccg cctgtcgcga aacagaagat
      301 caaggcgacc tcgtcgggta acggcgcgtc gggcagcact aggctcatcg tcccgtaatg
      361 cgagcggatg tagacgtcgt cgatggagtt catcggcaca tcgatcatcg taccgggtgc
      421 ggcaaccttg gtcaccgacg aaatccaggc gtcgccaccg ccgatcgcat cgcgaatggg
      481 gttggcgaaa accgtggtca gcagcgcatt cgcgtgttcg gtttcgccgt tcaggccgac
      541 aatgccagcc ttgccgtaag cttcaacagg cgcaccgccg agcacagcaa ccgcctgcgc
      601 gcccattttc tttccgaggc cgatactcgc ctcggtgagc gcggaaagat cttctacgta
      661 atgaccggcg taaggatttt tgagcaccat gatcgtggcg acttttctca acggcctttc
      721 agcccggcgc cccgcttcgt aaaaatgatc ctctatgatt gtcgcaactc tgcgaatctg
      781 catatccatc tatatctgcc cacccgtcgc tcatcagtca ccttcactgt tcatattgta
      841 tgacaacctg tcaataggcc agaaatgcgg cgctatttca cagcctcgaa tgaacgtcag
      901 cgcggcgccg ccggaataaa gaccctactt taggccacct cgcaaaatct tgaggaacgc
      961 catggtctcg tttatgctta acccgggatg gggctcccga ttgcagaaaa cagaaaaagc
     1021 ggttggcgga ctagtctcct caggtcatga ctgaattcgg tcgtgagcct cggcattgcg
     1081 aatgttgaat tcatccggag tttcaatgga tttcaaagtc ttgcctcaga gcgtgcaggg
     1141 acaggcggtt agcaaattac gcgacgcgat cgtgaccggt atttttcggc ccggcgaccg
     1201 cctcgtggaa tcggaccttt gcacacggct cggcatcagc cgcccttcgg tccgcgaagc
     1261 gctgcgaagt ctcgaagcgg aacgtctcgt ggtcatcgtg cccaatcgcg ggccgatgat
     1321 tccgatcctg accgtcgaca tggcgcggca gatttacgat gtccgtgctt tgcttgaggg
     1381 ggaagccgca gcgctttttg cagcccacgc accgccctcg gcgattgccg ccatgcgttc
     1441 cgccctggaa gcattcgacg ctgccgtcaa aagcggcgat ctgaccgaag agatcagggc
     1501 gacgcgccaa ttttacgagc cgatgctgct cggctgcggc aaccgcgtga tcttcgaagt
     1561 gctggatacg ctgagcgccc ggatcacctt cctgcgttca aaatctatgt cgcttccggg
     1621 ccgtgcacag aagagcgcac gcgagatgcg ggcgatcctg aaagggatta cctccggtga
     1681 cgcagaggca gcgcggacgg ccgccgtgac gcacgttcgc caggcctgta acgctgccgt
     1741 caaagcctat tccgcagaca agattgcaga aaagaccaca aaaaccagcg gctgacgtag
     1801 cgttaccctc cgcgtcagca ttccgcg
//
LOCUS       DASWOG010000370         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_283380,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000370 DASWOG010000000
VERSION     DASWOG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283380"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..>1684)
                     /locus_tag="VGB93_07710"
     CDS             complement(236..>1684)
                     /locus_tag="VGB93_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0146023.1"
                     /translation="ATAAMKSLNAYNLWLHGQSRSDPALLGAATTFTAAILLGRIATI
                     LHVGDSRAWHLRADHLTLLTDDHSAPQADGSRRLLRAIGLESALRLDVKAQPLEAYDR
                     LLLTTKGVHSVLSANALLKLLSARQTPQADADAIVEAALQAGSEDNATALVIDIITVP
                     APNYSAVVAEMENLPIKNLPAIGDTIDGFKLARILTESKTSRLILARDHNEWVVLKFP
                     KPEADKVDRTHFMREVFLGQRINHLNVGASLKIPEGRQSQLYVAMPYYRGETLEDRLQ
                     RDGPMKIDEAVAIAIKVGRGLIALHKAGVTHRDIKPGNIMLLENGDVKIFDFGVARLP
                     RLDDLDEAASPGTLDFMAPELFQEERGDALSDQFALGVTLYRILSNHYPYGETLPGER
                     PLFGPATPLSGYRKDIPAWLNAAVLRAISLRRDDRFGDLHEFIYALEHGNLRPPPRYR
                     RPFIEQNPLLFWKVLSALLAVLLILSIVWKRL"
BASE COUNT          369 a          457 c          506 g          352 t
ORIGIN      
        1 cgatgtctgc tggctcctcc cttcgtgacg cgttcgcgtc tttatgattc gcccggaaca
       61 tggcggctga tcacggcacg agcaaggttc ggcggcggct tttttgaccc cacagcatca
      121 ccgaaacgtg acgctgtctg cggcagaagc gacagctaaa gcagcgaacc gaaaagtgcg
      181 aagcggtttt cggacaaatc cgatgcttta aacgtccttc gcgttaagca cgcgatcaga
      241 gacgtttcca gacaatgctc agaatgagaa ggacagcgag aagagccgaa agaaccttcc
      301 agaagagcaa cgggttttgc tcgatgaagg gccggcgata acgcgggggt gggcgaagat
      361 ttccatgctc caatgcataa atgaactcat gcaaatcacc gaagcgatcg tcccgacgca
      421 atgaaattgc gcgtaggacc gcggcgttaa gccaggccgg gatgtccttg cgatatccgc
      481 ttagcggggt cgcgggaccg aaaagcgggc gctcgccggg cagcgtttca ccgtaaggat
      541 aatggttcga aagaatacgg tacaatgtga cgcccaaggc aaattggtcc gataaagcgt
      601 cgcctctctc ctcctggaag agttcaggcg ccatgaaatc gagcgtgccg gggctcgcgg
      661 cttcgtcaag gtcgtcgaga cgcggcagac gcgcgacacc aaaatcgaaa atcttcacgt
      721 cgccgttttc cagcaacatg atatttcccg gtttgatgtc gcgatgtgtg acgcctgctt
      781 tatggagcgc gatgagtccg cgtccgactt tgatcgcgat cgcaacggcc tcgtcaattt
      841 tcatcgggcc gtcgcgctga aggcgatctt ccagggtttc gccgcgataa tagggcatcg
      901 cgacatagag ctggctttgg cgtccctccg ggattttcag agatgcgcca acgttcagat
      961 gattaatacg ttggccgaga aatacttctc gcataaaatg cgtgcgatcg actttatcag
     1021 cctcgggctt ggggaatttc aacaccaccc attcattgtg atcgcgcgcc aaaatgaggc
     1081 gcgaggtttt gctctccgtg agaatgcggg caagcttaaa tccgtcaatt gtgtcgccaa
     1141 tcgccggcag gttcttgatt ggtaaattct ccatctcggc gacgacagcg ctatagttag
     1201 gggcgggcac cgtgatgatg tcgatgacga gtgctgtcgc gttatcttcg ctgccagctt
     1261 gcagtgcagc ctcgacaatc gcatcggcat cggcctgcgg ggtttgtcgc gcgctgagaa
     1321 gtttcagaag tgcgtttgcc gaaaggacag aatggacgcc tttcgtcgtg agcaaaaggc
     1381 gatcatacgc ctcaagcggc tgcgctttca catccagccg gagcgccgac tcgaggccga
     1441 tcgcgcgcag cagtcgccgg ctgccatccg cttgcggggc ggaatgatcg tctgtcagca
     1501 gcgtcagatg gtcggcgcgc agatgccatg cccggctatc gccgacatgc aggattgtcg
     1561 cgattcggcc caatagaatc gcggcggtaa aggtcgtcgc agccccgagc agcgccgggt
     1621 cggatcgcga ctggccatga agccaaagat tgtaggcgtt gagcgatttc atcgcagcag
     1681 tggc
//
LOCUS       DASWOG010000371         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_228933,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000371 DASWOG010000000
VERSION     DASWOG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228933"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB93_07715"
     CDS             <1..291
                     /locus_tag="VGB93_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146024.1"
                     /translation="LATFVAAFASYEGGFFLISLLTRSGLYAYAPSIVQRIFETNALA
                     FGGLLLIRRIAMALRKPAVKIRNAIPPVADDLHPRLNYDLVDAKAPGRAPGR"
     gene            complement(327..1538)
                     /locus_tag="VGB93_07720"
     CDS             complement(327..1538)
                     /locus_tag="VGB93_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HEY0146025.1"
                     /translation="MRAGTYEGVRFKADVTAAPIPRWDLLKFDQYLHVGVQFSRGCPF
                     TCEFCDIIELYGRVPRTKTALQVIGELDSLYALGYRGHVDFVDDNLIGNKRAVKAFLP
                     HLKAWQEKHHYPFDFSTEASVNLADDDELLGLMRDASFFAVFIGIESPDNETLIKMQK
                     KQNTRRVLSESIRHIYDAGIFVMGGFIVGFDTEMDAVAGPIVKLIEDASIPIAMVGLL
                     YALPNTQLTRRLEQEGRLFEPENGDINGDQCTAGLNFVMLRARRDALTDYRTVLDSIF
                     SPVAYFDRVRGVALSLKMPKRSLRVALGSTFREFRRFLRLITRVTLYRPDMRAQAWRT
                     LITILLRNPTAVTSAMRLTAFYVHLGPFSRFVVGRIDAQIGAIDAGRWIQPPLVSLPA
                     AAEPAIAELVA"
BASE COUNT          334 a          453 c          478 g          319 t
ORIGIN      
        1 ctggcaactt tcgttgccgc ctttgcgtct tacgaagggg gcttctttct tatttcgctg
       61 ctgacgagaa gcgggcttta tgcctacgcg ccgtcgattg tccaacgcat cttcgagacg
      121 aacgcgttgg ccttcggtgg gttgctactg attcgccgga tcgcaatggc gctaaggaag
      181 cccgccgtga aaatccgcaa tgccattccg cctgttgccg acgaccttca tccaaggctg
      241 aactacgatc ttgtcgacgc aaaagcgcct gggagggcgc cgggaaggta attcgcccga
      301 gttcaaagga tgaacaattt cgccactcag gcgacgagtt cggcaatggc tggctccgct
      361 gctgccggca aggacaccag cggtggctgg atccaccggc ccgcatcgat cgcgccgatc
      421 tgcgcatcga tcctaccgac gacgaaccga gagaaggggc cgaggtgcac gtagaaggct
      481 gtgagccgca tcgcagaggt gacggcggtg gggttgcgga gcagaatcgt gatcagggtc
      541 cgccaagcct gcgcgcgcat gtccggtcgg tacagcgtaa cgcgggttat gagccggagg
      601 aatcggcgga attcgcgaaa tgtgctaccg agtgccacgc gtagtgatcg cttcggcatt
      661 ttcaggctca aagcgacgcc cctcacgcga tcaaaataag ccaccggcga gaaaatgcta
      721 tcgaggacgg tgcgataatc cgtgagggcg tcgcgccgcg ctcgtaacat cacgaagttc
      781 aagccggcgg tgcactgatc gccatttatg tcaccgttct ccggctcaaa caggcgcccc
      841 tcttgctcaa gccgacgggt caattgggtg ttcggcagcg cgtagagaag gccgaccatc
      901 gcgataggga tgctggcatc ctcgatcaac ttaacgatcg gaccggcaac tgcgtccatc
      961 tcggtgtcga agccgacaat gaagcctccc atgacgaaga tgccggcatc atagatatgg
     1021 cggatgctct cggagagtac acgccgcgta ttctgtttct tctgcatttt gatcagtgtt
     1081 tcattgtccg ggctctcgat gcctatgaac accgcgaaga aactggcgtc acgcatcaaa
     1141 ccgaggagct cgtcgtcgtc agctaggttc accgacgctt ccgttgagaa gtcaaacggg
     1201 tagtggtgtt tctcctgcca ggccttgaga tgcggcagga acgctttaac agcccgcttg
     1261 ttgccgatca gattgtcgtc gacgaagtcg acatggccgc gatagccgag cgcgtaaaga
     1321 ctatctagct cgccgatgac ctgtagggcg gttttcgtgc gaggcacgcg gccgtatagt
     1381 tcgatgatgt cgcagaactc gcaagtgaag ggacacccgc gagagaactg cacgccgaca
     1441 tgcagatatt gatcgaattt caagagatcc cagcgcggga ttggcgcggc ggtgacgtcg
     1501 gccttaaagc gaacgccttc ataggtgccg gctcgcacgc ccgcgcgcca atcggcgaca
     1561 aacccgtcta tgagcccttc tgcc
//
LOCUS       DASWOG010000372         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_356640,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000372 DASWOG010000000
VERSION     DASWOG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356640"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VGB93_07725"
     CDS             complement(<1..788)
                     /locus_tag="VGB93_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0146026.1"
                     /translation="MLTIKHVDGPLAGQEQTISDDQAQVNVGRDPDRCQIVYPPDSTI
                     VGRVHCGLLRQPSGDWAVDLYGDHYVEIDGVAAEPRQTIRSGAKIRLGRHNGPSFIAN
                     ITHAASDDLGMTGAQQKQVSVRLVIRRDVRVAIGLVALAVFAAVGTLIYNARLSQQIR
                     QRLDRAAFLVEIRHQAGGCPDWDATAWPVSRDLLVTSAHVVDEAGAGTMLVRAPGADG
                     KEYEVIGHVLHPGYKAFADFFRRQDLVKWDDGQLETPPTGLACDV"
     gene            complement(858..2720)
                     /locus_tag="VGB93_07730"
     CDS             complement(858..2720)
                     /locus_tag="VGB93_07730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0146027.1"
                     /translation="MSDGAPSVGLGAELDRVFADLAANEPEAEIIGRLRGLEPGCNAP
                     NRDRARFLNARGLVMNRMGQRSEALGDLLESASVFTQIGDASGAAQILRSLALVHSWR
                     SDARESALALLRAISEASDDRINLALSFFEAGRLELEIGRPRDAQRFFQRGLDIGGDA
                     PPALERDRARVGGLQALVATRDLGGAQAAAAALNIEDLATGRLKHLARIELARLALAQ
                     NNFDDADATLADVRAGLPVDPDAFEAVEYAHVAAESLFARKANVEAQATIARVIARYA
                     DDNLPGREIAARLLDSQILDALEKPEEADRTLAAALRRAVATNLPGHADVVRERLAAR
                     GRSQNVWLPGLSPAPSLSATERFVRRRPLGAGGFGAVSRAYDLELGTEIALKNLRLKA
                     LYDSAVLAERRAGAQMEVAAASRIQHPGVGRVYGLLDEPDGDALLARELVEGPTLREA
                     MQSKLHLAEKIGLLAQLAHCLAAIHAADIIHRDLKPENVVLRDGISPVIIDFGISAIG
                     HVRQDKDAPKTLNYAAPEQSKNGWADARSDIYAMGVIAYEVLTGQVPPAPAHLAFFDI
                     RRKKLMGDLRRAGVPIAATRLIARMLSMSPRRRPQTAREIALNLQIALSGMQKA"
     gene            complement(2717..4723)
                     /locus_tag="VGB93_07735"
     CDS             complement(2717..4723)
                     /locus_tag="VGB93_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146028.1"
                     /translation="MAQSDQLANTRAKPRLALRIGVTGARNLATTHLSHIHAKVRAAL
                     TCFSDEMNSIAWERAVARFYASDDKKTCAPILRIISPLALGADRLVAEEALALGYKLY
                     APMPFSRTEYEKDFIGTDPAKTPHALPMTAAEDLDQFRTLLARAGEDWMSLDGDYDKE
                     KPDRDEQRNRAYEAVGRFVVRNSDILIAIWNGELAAGRGGTQEVVEYAANVGVPVWWI
                     HATEDTEPHWIADIHDARNPSASPKAAHKIHAYLRRQILEPKPVPRGHQTGVVSIARW
                     WQSKTVEPAQDYFNEQPNRPWCWSGIYNEMMLFASGCPPLWTPPTEPKSDAGIYWFGL
                     YQPADARASEYTARYRSSYVWIFTLTALSLLSGAIANFFHKRDITIFGHLIESRVSVT
                     TFAGIEFLLLSVTFCIAVLALRRDWHEHSIEYRLLAELCRKQQVLAPLGRAISFGAVR
                     RLASQAQETEEIAPRDSASSSTGHARAPDRAAWVSSLFMAYQRAAPLPRGNIAAHLKG
                     VLSDDVLQDLVDEQLQYHASRAQTSAAAAEYFEKLGGRIFVAVLIFVFIKIALTLGMH
                     PPESWQEELSTALLGLLGVVLPAWSAAAVGMGSYAEWRALAEESNHMLSLLNAARRRI
                     KRIDLKCPLASQDLGAEADAVASTMLQDLEGWQRLFRVKAIETQ"
     gene            4911..>5136
                     /locus_tag="VGB93_07740"
     CDS             4911..>5136
                     /locus_tag="VGB93_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146029.1"
                     /translation="MPKYSWMALVGVGLLSGCGSMTGPPSITASRTFYNEVIHDTSSE
                     QLLLNIVRAKNYETPNFIDVSEADATVSFTS"
BASE COUNT         1028 a         1612 c         1532 g          964 t
ORIGIN      
        1 acatcgcagg caagtccggt cggcggcgtt tcaagctgcc cgtcatccca tttcaccaaa
       61 tcctgacgcc ggaaaaaatc ggcgaaggct ttatagcctg gatgcaatac gtggccgatg
      121 acttcatatt ccttgccgtc ggcgccgggc gcacgcacaa gcatggtccc ggccccggct
      181 tcgtcgacga catgcgcact ggtcacgagg agatcgcggc tgaccggcca tgcagtcgca
      241 tcccaatccg gacatccgcc agcctgatgg cggatttcca cgaggaacgc ggcgcgatcg
      301 agccgctggc gaatttgttg gctcagccgt gcgttataga tcagcgtccc gacagcagcg
      361 aaaacggcca gcgcaacgag gccgatcgca acgcgcacat cacggcgaat gacaaggcgc
      421 accgagacct gcttctgttg cgcgccggtc atgccaagat cgtcacttgc cgcgtgggta
      481 atattggcga tgaaactcgg cccattgtgc cgtccgagac gaattttcgc gccgctgcgg
      541 atcgtctggc gtggctcggc cgcgacgcca tcgatctcga cataatgatc gccgtagaga
      601 tcgacggccc aatcgccgga aggctgccgc aacagcccgc aatgtacccg gccaacgatc
      661 gtgctgtcgg gcggatagac gatctggcag cggtcaggat cgcggccgac gttcacctgc
      721 gcctgatcgt cggagatcgt ctgctcctga cccgccaatg gcccgtcgac atgcttgatc
      781 gttagcaagg tccgccctca acgctgcgcg gcaacggtcc agttttagca aaatccgccg
      841 gacctgcgat agggcgctta ggctttttgc atccccgaga gggcgatttg cagattgagc
      901 gcgatttcgc gcgccgtttg cggccttcga cgcgggctca tggacagcat tcgtgcgatg
      961 agccgggtgg cggcgatggg aacgcctgcg cggcggagat cgcccatgag tttttttcgg
     1021 cgtatgtcga aaaatgcgag atgcgcgggc gcaggtggca cctgtcccgt caagacttca
     1081 taggcgatga cgcccatcgc atagatgtcg ctgcgcgcat cggcccagcc attcttgctt
     1141 tgctcgggcg cggcataatt cagcgtcttc ggcgcatcct tatcctggcg gacatggccg
     1201 atggcgctga tgccgaaatc aatgatgacc ggcgatatcc catcgcgcag gacaacattc
     1261 tccggcttca gatcgcggtg aatgatgtcg gcggcgtgga ttgcggcgag acaatgcgcc
     1321 aattgcgcga gaagcccgat cttctccgcc agatgcagct tgctctgcat cgcctcgcgc
     1381 aaggttggcc cctcgaccaa ttcgcgcgcg aggagcgcgt cgccatccgg ctcatccaac
     1441 aatccgtaga cgcgcccgac gcccggatgc tgaatgcgcg aggctgccgc tacttccatc
     1501 tgcgcaccgg ctcggcgctc ggcgagtacg gcgctgtcat aaagcgcctt gagccgaagg
     1561 ttttttaggg caatctcggt acccagttcg agatcatagg cgcggctgac cgcgccgaag
     1621 ccacctgccc ccaacggccg gcgccggaca aagcgctcgg tcgcgctcag actaggcgcc
     1681 ggagacaggc ccggcagcca gacattctgc gagcgcccgc gagccgcgag ccgctcgcgc
     1741 accacatcgg cgtggcccgg cagattggtc gcgacggcgc ggcgcagcgc cgccgccaaa
     1801 gtgcggtccg cctcttccgg cttttcgagc gcatccaaaa tctggctgtc gagcaggcgc
     1861 gcggcaatct ctcgacccgg cagattgtca tcggcataac gcgcgatgac ccgggctatg
     1921 gtcgcttgtg cctcgacatt ggctttccgc gcgaagaggc tttccgcagc gacatgcgca
     1981 tattccaccg cctcgaacgc gtccggatcg accggcagac ctgcacgcac atcggcgaga
     2041 gtggcgtcgg catcatcaaa attattctgc gccagcgcga gccgcgccaa ttcgatccgc
     2101 gccagatgtt tgaggcgccc cgttgccagg tcttcgatat tcaaagccgc cgcagcggct
     2161 tgcgcgccgc cgagatcgcg cgttgcgaca agggcctgca agccgccaac gcgggcacgg
     2221 tcgcgctcca atgccggcgg ggcatccccg ccaatgtcga gacctcgctg aaagaaacgc
     2281 tgggcgtcgc gtggccggcc gatttcaagt tcgagccgtc cggcctcgaa gaacgacaac
     2341 gcgagattga tacggtcgtc gcttgcttca ctgatggcgc gcagcagcgc gagggcagac
     2401 tcgcgcgcat cgctgcgcca ggaatgaacc agcgcaagcg agcgcaggat ttgcgcagca
     2461 cctgaggcgt cgccgatctg cgtgaagacc gaggctgatt cgaggaggtc gccgagcgcc
     2521 tcgctgcgct gacccatccg gttcatcacc aggccacgcg cgttgaggaa gcgtgcccgg
     2581 tcacggttcg gcgcgttgca gccaggttcg aggccgcgca accggccgat gatttcggcc
     2641 tcgggctcgt tggcggcgag atcggcaaag acccgatcga gttccgcgcc aagaccgacg
     2701 gacggcgcgc catcactcat tgcgtttcaa tggccttgac gcggaagagc cgttgccagc
     2761 cttccagatc ctgtagcatc gtcgaggcga ccgcgtccgc ctcagcgccc agatcctgcg
     2821 aggccagcgg gcattttaga tcgatgcgct taatgcgccg cctcgctgcg ttgagcaggc
     2881 tgagcatgtg gttcgattct tcggccaaag cccgccattc cgcatagctg cccataccga
     2941 cggcagctgc cgaccaggcc ggcagaacga cgcccaaaag accaagaaga gccgttgaca
     3001 gctcttcctg ccaggattct ggcggatgca tacccaaagt cagtgcaatt ttgatgaaaa
     3061 cgaagataag cacagccacg aaaatccggc caccaagctt ctcgaagtat tctgcagccg
     3121 cggccgatgt ttgtgcccgg ctggcgtgat attgcaattg ctcatcaaca agatcctgga
     3181 gcacatcatc gctcaagacg cccttcagat gagccgcgat attgccacgc ggcaaaggtg
     3241 ccgctcgctg ataggccatg aaaagcgagc tgacccaagc cgcgcggtcc ggcgcgcgcg
     3301 cgtgaccggt tgagctggac gcactgtcac gaggcgcaat ctcttccgtt tcctgagcct
     3361 gggaggcaag ccggcgcact gccccaaacg agatggcccg cccgagcggc gcgagcacct
     3421 gctgtttgcg gcaaagttca gcgagcaggc ggtattcgat cgagtgctcg tgccaatctc
     3481 gacgcaacgc taaaactgcg atgcagaaag tcacagacag aagaaggaac tcgatgccgg
     3541 cgaaagtcgt gacgctcacc cggctttcaa tcaaatggcc aaagatcgtg atatctcgct
     3601 tgtgaaaaaa attggcgatc gcgccggaca gcaacgacaa cgcggtaagc gtgaatatcc
     3661 agacataact cgaacggtag cgcgccgtat attcgctggc acgcgcgtcc gccggctgat
     3721 aaagcccaaa ccaataaata cccgcgtcgc ttttcggctc tgtcggcggc gtccatagtg
     3781 gcgggcagcc cgaagcgaag agcatcattt cgttatagat tccagaccaa caccacggtc
     3841 gatttggctg ctcgttaaaa taatcttgcg ccggctcgac tgtcttggat tgccaccagc
     3901 gcgcaatgct cacgacgccg gtttgatgtc cgcgcggaac gggcttcggt tctaaaatct
     3961 gccgacgcag gtaagcgtga atcttgtgcg ctgctttcgg tgaagctgac ggattgcgcg
     4021 catcgtggat atccgcgatc caatgcggct cggtgtcttc cgtcgcgtga atccaccaga
     4081 ccggcacgcc gacgtttgcg gcatattcga cgacctcctg cgtgccgcct cgaccggccg
     4141 caagctcgcc gttccagatc gcgatgagga tatcgctgtt gcggacgacg aaacgaccga
     4201 cggcctcata agcgcggttg cgctgctcat cgcgatccgg cttttccttg tcgtaatcgc
     4261 cgtcgagcga catccagtct tcacccgcgc gcgcgaggag cgtccggaac tggtcaagat
     4321 cctccgctgc cgtcatcggc agggcatggg gtgttttcgc gggatccgtt ccaataaagt
     4381 ccttctcata ttcggtacgc gaaaacggca tcggcgcata aagcttataa ccgagcgcga
     4441 gcgcctcttc ggcaacaaga cgatcggcac cgagcgcaag cggcgaaatg atacgcagaa
     4501 tcggtgcgca tgttttttta tcgtcgcttg catagaaccg cgccacggcg cgctcccacg
     4561 cgatcgaatt catttcgtcg ctaaaacatg tcaacgccgc gcgcaccttg gcgtgaatat
     4621 gggaaagatg cgtcgtcgcg agattgcgcg cgcccgtgac accaatgcgc agcgcgagcc
     4681 tcggcttggc ccgcgtattt gccagctgat ccgattgagc caagtgcagc accgcaagca
     4741 acgatctagc tttggcggtt tgtgccatgc cgcgtcgact taaacaagcc gacgcggcca
     4801 aatcagatgc aaaattacaa cattcggcaa caatgctttc cctgccattg actcaaattc
     4861 gcgctgccga tcatgtcaat ggctccggtt gcggtgtatc gggggttgcc atgccgaagt
     4921 acagctggat ggcgctcgtc ggcgtaggcc ttttatccgg ctgcggttca atgacgggcc
     4981 cgccatcgat tacggccagt cggacgtttt acaacgaagt cattcacgat acgtcgtccg
     5041 agcaattgtt gctcaatatc gtccgcgcca agaactacga gacgccgaat ttcatcgacg
     5101 tttcagaggc cgacgcgaca gtctcgttta cgtctg
//
LOCUS       DASWOG010000373         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_356687,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000373 DASWOG010000000
VERSION     DASWOG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356687"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGB93_07745"
     CDS             complement(<1..444)
                     /locus_tag="VGB93_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146030.1"
                     /translation="MILYKEGIGGEMKIIAIVVAGVVGLSVLVAAYVRGVELRGRSSI
                     DRVLADPLVAKVRFGDSTYAVPRRYLIGVSQPTSTSTYASIYIQMALPPQIAGQSSQV
                     DQRGWGIGSREIYFKATSKGSLFFTCDDKEEFPAIKYPSCTVNEYM"
     gene            complement(473..1393)
                     /locus_tag="VGB93_07750"
     CDS             complement(473..1393)
                     /locus_tag="VGB93_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146031.1"
                     /translation="MKRATIKQLESVRCVAEAGTIVKAAEHLNVTPAALTSLIQLLDQ
                     DAGVALFDRTGGRLRLTVAGEEVVCAAHRIERVLTDLDVTIAALRGTHSGRIAVGIVS
                     TAKYIAPRLVAAFARQNPRVDVTITVGNRLEMVNQLRDYKIDVCLIGRPPSDFPVESV
                     AIGEHPQVIVAAPDHPLANCCRIAKADLAKENFIIRETGSGTRAVFDYFFSDVPVRLP
                     TVNIEIGSNETIKQAVMAGLGLALLSAHTIEAEIASRRLAILDVVGLPLLRQWFVVRR
                     ADCAPTAIAQAMWDFAVAEAPKLLPNGIAA"
     gene            1506..1883
                     /locus_tag="VGB93_07755"
     CDS             1506..1883
                     /locus_tag="VGB93_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146032.1"
                     /translation="MLKVAQRHHLEDVLQDMTARDAAFAPVAALVHEIAHAAVEMADL
                     ISLGLLGGDGAAAVGDVNAGGDVQKHLDVFGNDLFVNALRGAPVAAVLSEELEEPLVL
                     DPQATLAVAMDPVDGSSNINANV"
BASE COUNT          410 a          597 c          558 g          421 t
ORIGIN      
        1 catatattca ttaacagtgc atgatggata tttaattgcc ggaaactcct ctttatcatc
       61 gcaagtaaaa aataaactcc ccttcgatgt agccttgaaa tatatttcac gcgagccgat
      121 cccccagcct ctttgatcga cttgtgaaga ttgccccgca atctgcggag gcaacgccat
      181 ctggatgtaa atggacgcat acgtgctcgt gcttgtcggc tgactgacgc ctataagata
      241 acgacgcggg accgcatagg tactgtcacc aaaacgtact ttggcaacga gtgggtcggc
      301 taacacacgg tcaatcgacg aacgaccccg taattcaact ccccggacat aggccgctac
      361 taaaactgac agacccacga cccctgcaac tacgatcgcg attattttca tctctccccc
      421 aatcccctcc ttgtagagga tcaatggtga gagcagattg tttatgtcgc agctaagctg
      481 cgatcccgtt cggcagaagc ttcggcgctt cggcgacggc gaaatcccac atcgcttgtg
      541 caatcgccgt cggcgcgcaa tcggcgcggc gcacgacgaa ccattgtcgc agcagcggca
      601 ggcccacaac atcgaggatc gccaagcggc ggctcgcaat ctccgcttcg atcgtatgcg
      661 ccgacagcag cgccaagccg agcccggcca tcaccgcttg cttgatcgtc tcgttcgagc
      721 cgatctcgat attgaccgtc ggaagccgca ccggcacgtc ggagaagaaa taatcgaaca
      781 ccgcccgcgt gcccgagccg gtttcgcgga tgatgaaatt ctctttggcc agatcggcct
      841 tcgcgatacg gcagcagttt gcaagcgggt gatcgggggc ggcgacgata acctgcggat
      901 gctcgccaat ggccacgctc tcgaccggaa agtcgctcgg cggccggcct atcaggcaaa
      961 cgtcgatttt atagtcgcgc aattgattga ccatttccag ccggttgccg acggtgatcg
     1021 tcacatcgac gcggggattt tgccgcgcga aggccgcaac gagacgtggc gcgatatatt
     1081 tcgcggtcga gacgataccg accgcgatgc ggccgctatg cgtgccgcgc aacgccgcga
     1141 tcgtcacatc gagatcggtc aagacgcgct cgatgcggtg ggccgcgcag acaacctctt
     1201 ccccggcgac agtcagccgc aggcgaccgc cggtgcgatc gaataaagca acgcccgcgt
     1261 cttgatcaag caattgaatt agtgaggtca gtgccgcagg cgtgacgtta aggtgctccg
     1321 ccgccttcac aatcgtcccg gcttcggcaa cgcaacggac gctttcgagt tgtttgatcg
     1381 tggcgcgctt catccactat tattggattt tttttgatgc tatctcaacg gaattcattt
     1441 ttctttggcc cctcgcgggc tgacttcggt ccataagact tcacagaata agcgcagggt
     1501 cggacatgct caaagtcgcg cagcgccatc atctcgaaga cgtcttgcag gacatgaccg
     1561 cgcgggatgc cgcgttcgcg cccgtcgccg cgcttgtcca cgagatcgca cacgccgccg
     1621 tcgaaatggc ggatttgatt tcgctcggcc tgctcggcgg tgacggtgct gctgccgtcg
     1681 gcgacgtcaa tgccgggggc gatgtgcaaa agcatctcga cgtgtttggc aacgacctct
     1741 ttgtcaacgc gcttcgcggc gcgcctgtgg ctgctgttct gtcggaagaa ttggaggagc
     1801 cgctggttct cgacccgcag gcgacgctcg ccgtcgcgat ggacccggtt gatggatcgt
     1861 ccaacatcaa tgccaatgtt tagatcggta cgatcttctc ggggctgccc gtcgtgtcgt
     1921 gcgccagcga cgccagagcg tattttttgc agccgagccg taaccaattg gccggcgggt
     1981 tcatca
//
LOCUS       DASWOG010000374         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_247053,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000374 DASWOG010000000
VERSION     DASWOG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247053"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB93_07760"
     CDS             complement(<1..194)
                     /locus_tag="VGB93_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146033.1"
                     /translation="MLSRQEGSFVPTRSALIQIVFVLVLAFIVYEGVATALAHMRAHH
                     IPYDFGFWSQRAGFDIDQRL"
     gene            complement(209..1246)
                     /locus_tag="VGB93_07765"
     CDS             complement(209..1246)
                     /locus_tag="VGB93_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0146034.1"
                     /translation="MITLRKTIFCLSLGILSLGLAALFSSVASAGTRDQVKQRGYLNC
                     GSNPGLAGFGLPDSSGNWTGLDVDFCRAIAAAIFNDPNKVKFISLTAKDRFTALQSGE
                     VDVLAHNTTWTVSREAGQGLLFTGINYYDGQGFMIHNKLNISSALELSGASICVQQGT
                     TSELNLADFFHSHQMQFESVTFATSDEALKAYDTGRCDAFTTDSSALYIERLKVANPE
                     QNIILPEIISKEPFSPAVRQGDDQWFNIVKWVNFAMLDAEEAGITSKNVDDKMNSDDP
                     TARRLLGVEGNFGESLGLTKDWAYRFIKAVGNYGESFDRNLGAESKLGIKRGINSLWT
                     HGGIQYAPPVR"
     gene            1472..2653
                     /gene="metC"
                     /locus_tag="VGB93_07770"
     CDS             1472..2653
                     /gene="metC"
                     /locus_tag="VGB93_07770"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY0146035.1"
                     /translation="MSKMDERRPLKERTKLVHAGRHPSEQHGFINTPIYRGSTVLFPS
                     YADLITRNARYTYGTHGSPTTDALETAWTEITGAAGTVLAPSGLSAVALAILTAVKAG
                     YHILVSDSVYQPTRNFCERFLTRMGVETTFYDPWLGANIETLVRLNTSAIFLETPGSQ
                     TFEIQDVPAIAAVAAAKGLCTILDNTWATPLFFPPHRFGIDLAIEAGTKYLSGHADLL
                     LGLVSANERWLKRLHTSFDFFANCPGPEDVFLALRGLRTLELRLREAERQGLDLATWL
                     AARPEVERVLHPALLDCPGHEIWKRDFKGSSGLFSAFLKPVSQPALAAFLDRLKLFGM
                     GYSWGGYESLVIPFDCSTYRTATKFNPPGPALRFSIGLEDPEDLKADLAAGFERLNAI
                     G"
     gene            2763..3665
                     /locus_tag="VGB93_07775"
     CDS             2763..3665
                     /locus_tag="VGB93_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146036.1"
                     /translation="MSDASHSHDDPVPLHGLEGSDVLRLALLSLAAIIAVALAVGLFM
                     DAGRQIEPRTAQLPVAAAAPATPSAPAPGASDEPLRQARESVEREIAEKAPEYAVFFD
                     RLHGALPTEYDTIVDGFAREALDGKDLSIDALLSQAVRDLRVSNGILAAKADGPALSH
                     IFDIQLRMMQALAATDPKLCVDFLYGGASQAFFAFSAAHRPLVADMAIAGLEAIGSGR
                     TNQIDRDAPTDTDFNQLSSAMKSQGVNDAEVAAILDGKTPNPPIPDARMCAVGQIYLK
                     TLAALPEQARLRIYALAVSLMARS"
     gene            3786..4004
                     /locus_tag="VGB93_07780"
     CDS             3786..4004
                     /locus_tag="VGB93_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146037.1"
                     /translation="MPNVYIEPRPKGHKEGHHIEDYVVEDHTDHVLHTTKTQHEAIEW
                     AKAQGHHPLVARVRHENDKKSPGHWRSA"
     gene            complement(4037..>4342)
                     /locus_tag="VGB93_07785"
     CDS             complement(4037..>4342)
                     /locus_tag="VGB93_07785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY0146038.1"
                     /translation="ADASFLAHGRARDGDADMQPVYLTCAAENPNGARLRLFIDGADA
                     AAHLAADAAYERAILLFDGNDSDQVGAARAQWKILKEQGVPITYWQQNERGGWEKKA"
BASE COUNT          890 a         1309 c         1260 g          883 t
ORIGIN      
        1 atcagacgct gatcaatgtc gaagcccgcg cgctgcgacc agaacccgaa atcgtaggga
       61 atgtgatgcg cgcgcatatg cgccagcgcc gtagctacgc cctcatagac aatgaacgcg
      121 agaacaagaa cgaaaacgat ctggatgagc gcgcttcgcg tcggcacgaa gctgccttct
      181 tgcctgctca acatattgct acgcgaaatc agcgtaccgg cggggcatat tgaatgccgc
      241 catgcgtcca gagcgaattg ataccgcgtt tgattccgag cttcgactcg gcgccgagat
      301 tgcgatcgaa ggattcgccg taattgccca cggccttgat gaaacggtag gcccaatcct
      361 tcgtcaggcc gagggattcg ccgaaattgc cttcgacgcc gagcagccgc cgggctgtcg
      421 gatcgtcgga attcatcttg tcatcgacgt tcttggacgt gattccggct tcctccgcgt
      481 cgagcatcgc aaagttcacc catttgacga tgttgaacca ttgatcgtcg ccttgccgca
      541 cggcagggct aaacggctct ttcgagatga tctccggcag aatgatgttc tgctcgggat
      601 tggcgacctt cagccgttcg atatagagcg ccgaggaatc ggtggtaaag gcgtcacacc
      661 tgccggtatc ataggctttc agcgcctcgt cggaggtcgc gaatgtcacc gattcaaatt
      721 gcatctgatg cgagtggaag aaatcggcaa gattcaattc gctcgtcgta ccttgctgca
      781 cgcaaatcga ggcaccggat aattccagcg ccgaagatat gttgagcttg ttgtgaatca
      841 tgaagccctg gccgtcgtaa taattgatgc cagtgaagag aagtccctgc ccggcctcgc
      901 gcgatactgt ccaggtcgta ttatgcgcca gcacatcgac ctcgccggat tgcaaggcgg
      961 tgaaacggtc tttggcggtg agcgaaataa atttgacctt gttcggatca ttgaagatcg
     1021 ccgcggcgat ggcgcggcag aaatcgacat cgagaccggt ccaattgccg ctcgaatccg
     1081 gtaggccgaa gccggcaagg cctgggtttg aaccgcagtt cagatagccg cgctgtttga
     1141 cctgatcgcg cgtcccggcc gaagccactg aggagaaaag cgccgccaga ccgagagaaa
     1201 gaatgccgag cgacaggcag aagattgttt tacgcaatgt gatcatgctc atttcggctc
     1261 cgggctggga acacggccgc gggcgcgcga ctatcagaac tttccagcgg cgcggtcaag
     1321 cggcgcgaca aattgtcgga acggccaagt tggcgcgaat ctgcgatttg ccgtaagcct
     1381 tggtccgcaa tgtcagcgtc aatctgacgt atcttgctgg caagtcctgc cggcatccgg
     1441 gacgtggcga tccttttccc aggacgaggc aatgtcgaag atggacgagc ggcggccgct
     1501 gaaagagcgc accaaactgg tgcacgccgg gcgtcaccct tccgagcagc acggcttcat
     1561 caatacgccg atctatcgcg gctcgaccgt tctctttccg tcatacgccg atctcatcac
     1621 gcgcaatgcg cgatatacct acggcacaca cggatcgccg acgacggacg cgctcgagac
     1681 agcctggacg gaaattacag gcgccgccgg cactgtgctg gcaccctcgg gcctgtccgc
     1741 tgttgcgctt gcgatcctca cggcggtcaa ggccgggtac catattctcg tcagcgattc
     1801 cgtctatcag ccgacacgca atttctgcga gcgctttctg acgcggatgg gcgtcgaaac
     1861 gacgttctat gatccttggc tcggcgcgaa tatcgagacg ctcgtaaggc tgaacacgag
     1921 tgcaatcttc cttgaaacac caggctctca gacttttgaa atccaggacg tgccggcaat
     1981 cgccgccgtc gcagcggcaa aaggcctctg cacgattctg gacaatacgt gggcgacgcc
     2041 actgtttttc ccgccgcatc gtttcggcat cgatctcgcg atcgaggccg gcaccaaata
     2101 tctttccggc cacgccgatc ttttgctcgg actcgtctcg gcgaacgagc gctggttgaa
     2161 acgccttcac accagcttcg atttttttgc caattgtccc ggccccgaag atgtctttct
     2221 cgcgctgcgg ggcttgcgca cactggaatt gcggctccgc gaagccgagc gccaggggct
     2281 cgatctcgcc acatggctgg cggcgcggcc ggaggtcgag cgggtgctgc atccggccct
     2341 gctggattgt cccggccatg aaatctggaa acgggatttc aaaggctctt ctggcctgtt
     2401 ctcggcgttc cttaaaccag tttctcaacc ggcgctcgct gccttcctcg ataggcttaa
     2461 actgttcggt atgggttatt cctggggcgg ctatgaaagt ctcgtgatcc ccttcgattg
     2521 cagcacctat cgcacggcga caaaattcaa tccgcccgga ccggccttgc gcttctcgat
     2581 cgggctcgaa gacccggagg atctgaaagc cgatcttgcc gcggggtttg aacgcctcaa
     2641 cgccatcggc tgacaaatcg cgcggagacg ctggccttcg ttctatcatc gttctttaaa
     2701 tcaacagctt acaatgcctg tgatgctttc tgaagtgttg ctcgccgccg gggtgtatcc
     2761 gtttgtccga cgcgtctcac agccacgacg atcctgttcc gctgcatggg ctcgaaggct
     2821 cggatgtcct gcgcctcgcg ctcttaagcc tggccgcaat tatcgccgta gctttggccg
     2881 tcgggctttt catggacgcc gggcgccaaa ttgagccgcg cacggctcaa ctacctgtcg
     2941 ccgcagcggc gcctgccacg ccatccgctc cggcgcctgg ggcttcagac gagccacttc
     3001 ggcaggcgcg cgaatccgtc gaacgggaga tcgccgaaaa ggcgccggaa tatgcggtct
     3061 tctttgaccg gctgcatggc gcgctgccta cggaatacga cacaatcgtc gatggcttcg
     3121 ccagagaagc cctcgacggc aaggatttga gcatcgatgc gctgctgtcg caggcggtgc
     3181 gcgatctgcg cgtgtcgaac ggcattcttg cggcaaaagc ggacggcccg gcgctgtcgc
     3241 atattttcga catccaattg cggatgatgc aggcgctggc ggcgaccgat ccgaaactct
     3301 gcgtcgattt tctatatggc ggcgcaagcc aggcgttctt cgccttctct gccgcgcatc
     3361 ggccgctcgt cgccgatatg gccatcgccg gactggaggc aatcggcagc ggccgtacca
     3421 atcagatcga tcgcgacgca ccgaccgata ccgatttcaa ccagctttca agcgcgatga
     3481 aatcgcaggg cgtcaacgat gcggaagtcg ccgcgatcct cgacggcaag acgcccaatc
     3541 cgcctattcc agatgcgcgc atgtgtgcgg tcggccagat ttatctgaag actctcgccg
     3601 cgttgcccga gcaggcgcgc ctgcgcatct atgcgctcgc cgtgtcgctg atggcgcgtt
     3661 cgtaaagcgt ccggcccgaa gcgctcgctt catatgacag ccagagacta gaaaacatcc
     3721 cggcttcacc acgcaagtta tatgctatca aatacaggca agtagtaagt catggggagt
     3781 gaccgatgcc aaacgtctat attgagccgc gcccgaaggg ccacaaggaa ggacaccaca
     3841 tcgaggatta tgtggtcgaa gatcataccg accacgtgct gcatacgacc aagacccagc
     3901 acgaagcgat cgagtgggcg aaggcgcaag gccatcatcc gctcgttgcg cgcgttcgcc
     3961 acgagaacga taagaaatcg ccaggccatt ggcgctccgc gtaaacaagc caaaaaaggg
     4021 gcgcggccat ttgaggctac gccttcttct cccagccgcc gcgttcgttc tgctgccaat
     4081 aggtgatcgg gacaccctgc tccttcagaa tcttccactg tgcgcgggcg gcgccgacct
     4141 ggtctgaatc gttgccatcg aacaaaagga tcgcacgttc gtaagcggca tcggcggcaa
     4201 gatgcgcggc ggcatcggcg ccatcgataa agagtcgcaa acgcgccccg ttcggatttt
     4261 cagcagcaca ggtcagatag acgggttgca tgtcggcatc gccgtcgcgc gcgcggccgt
     4321 gtgccagaaa actcgcatcg gc
//
LOCUS       DASWOG010000375         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_118697,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000375 DASWOG010000000
VERSION     DASWOG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118697"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VGB93_07790"
     CDS             <1..1757
                     /locus_tag="VGB93_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0146039.1"
                     /translation="LPESRGVGKGTALLEAYESTDAIFIFGQNPGTNSPRMMTTLRDA
                     SRRGVSIISFNPFRERSLERFQAPQSPIEMVTLTSTPISESLYQVRVGGDVAVLKGLM
                     KAMIEADDAAIAADAPRVVDVDFINGHTKGFDALATDLRAASWTAIERKSGLSRAQIC
                     SAAMVYMNAPTAILVFGMGITQHRYGTLAVQQIANLAMLRGNVGRPGAGVCPVRGHSN
                     VQGDRTVGITEKPTAEFLSRLEKRFGFKPPSAHGHDVVTALEAMIRGESKVFIALGGN
                     FAAAVPDWIQTRGALRKLDLTVQISTKLNRSHMVHGREAIILPCLGRSEIDVQANGPQ
                     AVTVEDSMSMVHASRGMNVPVSEHLRSEPAIIAGMARATLGPGSKVAWEDMIADYDQI
                     RLGIEAVFPIFQAYNDRIRVPGGFHLTSTARERIWMTPSGRANFLVFEGLDEDLPERD
                     PEILWLTTVRSHDQYNTTIYSMSDRYRGVFGQRDIVFLNTKEIAKRGLANGDRVDLTT
                     VSDDGIERVVSNLLIVEYKFPDGCCAAYYPETNPLIPLYARDPKSHTPSSKGVPVRLS
                     RAKAHTNKAAALSLAEAT"
     gene            1761..3179
                     /locus_tag="VGB93_07795"
     CDS             1761..3179
                     /locus_tag="VGB93_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0146040.1"
                     /translation="MSADILSRLQFAFTIGYHILWPTFTIGTSAFVAWLSILVWLTDK
                     PVYRDLLRFWRRLFALGFGMGVITGIVLSYEIGTNWAGFSRAVSNVVGPLLMYEAATA
                     FFLESGFIGIMLFGEGRVSRGLYVFACVMVSLGSLISATWIIAANSWMQTPSGAVADS
                     HGIFHVVSWWHVIFNPSFPYRLMHMVCASFLTCSFVVAGVSAFHLLQRRHVDAARTAL
                     SMALWAALVLAPLQILLGDMQGLNTRQYQPTKLAAMEGLWITEKDVPAAVFAWPDMKS
                     EKNLYEISIPHLASVYLTHTWDGEVQGLKAVPPRDRPYVPIVFFAFRIMVGVGLILLG
                     LAVTGAVLRWRGRLFETKWFQYLAVLATPLGFVSVLAGWTVTETGRQPFVVYGMLRTA
                     DAASPVAAGAITTTLVGFFVIYNILLLGFLYFAGRLVLKGPTWGPSYSGTFRPGLDRA
                     APTVFGAPAATPTNVGNLSPGE"
     gene            3185..4249
                     /locus_tag="VGB93_07800"
     CDS             3185..4249
                     /locus_tag="VGB93_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0146041.1"
                     /translation="MPLFFALLAAFSVALYVLADGFDLGVGILFLFAPRDIDRDRMIE
                     SIEPVWDGNETWLVFGGTLLWAAFPAAYFVLLPAFYLPILFMLFALIFRGVAFSFRAE
                     SGRFRRVWDFAFAGGSLLAAFCQGLVLGGFIGGVSMSNGMFSGGPFSFLSILGVLCGI
                     GLIGGYALMGAGWLIWKTEGSTQIFAREIGHAALILTTALMAIVSAWTAFSQPAVDQR
                     WFSWPSIAELGLVPLVTIGTLVATWRALWGTSDTRVFVLSIFTFLLGFLGLLVSLWPY
                     VVPRHITIWDSASDPQTLSFIGAGLIVIIPIVLAYQFHAYWVFRGKIPDHAIADDGIE
                     LNPGIHARHTCTQDNVLHLS"
     gene            4852..5088
                     /locus_tag="VGB93_07805"
     CDS             4852..5088
                     /locus_tag="VGB93_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146042.1"
                     /translation="MTVEDLPQYPQWKRALQNMIEAWESYKVMSHLFDQHPEALRRKS
                     NFEMAKAQYLRVAYELEMPGYLVVSARDVDFRHA"
     gene            5229..5363
                     /locus_tag="VGB93_07810"
     CDS             5229..5363
                     /locus_tag="VGB93_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146043.1"
                     /translation="MFEVISANSIIVWFVWGFFMALGWALGTWVMTWILGYVPLSRRR
                     "
     gene            complement(5406..>5927)
                     /locus_tag="VGB93_07815"
     CDS             complement(5406..>5927)
                     /locus_tag="VGB93_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146044.1"
                     /translation="ILGLGNIGSEVARIGRAFGMETIAWSERLTKERADEVGARLVSK
                     DELFRDADIVSIHLVLADATRGLVGARELSQMKPTAWLVNTSRGPIVDEHALVTALSG
                     RKLAGAAIDVFDIEPLPTKHPFRVLDNVLATPHIGYVSRSEYEVFYGETVEGVIAWLD
                     GRASQPSTNVTVP"
BASE COUNT         1098 a         1716 c         1697 g         1416 t
ORIGIN      
        1 gcctgccaga gtcgcgcggc gtcggcaaag gcacggccct tctcgaagcc tacgagtcta
       61 ccgacgctat cttcatcttc ggtcaaaatc ccggcacgaa tagcccgcgt atgatgacaa
      121 cgctgcgcga tgcatcgcgg cggggtgtgt ctatcatatc tttcaatccg ttccgggaac
      181 gctcacttga gcgcttccag gcgccgcaga gccccatcga aatggtgacg ctgacgtcca
      241 cgccgatcag cgaaagtctt tatcaggtgc gtgtcggcgg cgatgtcgcg gtgctcaaag
      301 ggctgatgaa agccatgatt gaagccgatg acgcggcgat agctgctgac gcgccgcgag
      361 tcgtcgacgt ggacttcatc aatggtcaca caaagggctt tgatgcactg gctaccgacc
      421 tgcgcgcggc gtcctggaca gcgatcgagc gaaagtccgg cttaagccgc gctcagattt
      481 gctccgcggc gatggtttac atgaacgcgc cgaccgcgat ccttgtcttt ggcatgggga
      541 tcacccagca tcgctatggc actctcgcgg ttcagcagat tgccaatctc gcgatgttgc
      601 gcggcaatgt tggacgaccg ggtgctggtg tttgcccggt tcgcgggcac tcgaatgtgc
      661 agggggatcg taccgtcggc atcaccgaaa agccgaccgc ggaatttctc agtcgcctcg
      721 aaaagcgctt tgggttcaaa ccgccgagcg ctcacgggca cgatgttgtc accgccttgg
      781 aagccatgat ccgtggcgag tcgaaagttt tcatcgcgct tggtggaaat ttcgccgcgg
      841 cggtgccgga ttggatacaa acccgaggcg cgctgcgtaa actcgatctc acagtgcaga
      901 tttcgacgaa actcaatcgc agtcatatgg tccatggccg cgaggcgatc attctccctt
      961 gcctcggccg ttccgagatc gacgtccagg cgaacggccc gcaggcggtc accgtggagg
     1021 attcgatgtc aatggtacac gcgtcgcgcg gcatgaatgt gcccgtgagc gagcatttgc
     1081 gcagcgagcc cgcaatcatc gccggaatgg cgcgcgcgac attgggtccg ggatcaaagg
     1141 tcgcctggga ggatatgatc gccgattatg atcagatacg gcttggcatc gaagccgtgt
     1201 tcccgatttt tcaagcctat aatgatcgga tcagagttcc cggaggcttc catcttacct
     1261 ccaccgcccg cgaacgaatt tggatgacac cttccgggcg ggcgaatttt cttgtcttcg
     1321 aggggctcga cgaagatttg cccgagagag acccggaaat cttatggctc accacggtgc
     1381 gtagtcacga tcaatacaac acgacgatct attcgatgtc ggatcgttat cgcggtgttt
     1441 tcggacagcg cgatatcgtc ttcttgaaca ccaaggagat cgccaaacgc ggcctcgcca
     1501 acggcgaccg tgttgatctg acgaccgtct cggatgacgg tattgagcgg gtggtgagca
     1561 atctgctgat tgtcgaatat aagtttccag atgggtgctg cgcggcctat tacccggaga
     1621 cgaatcctct cattcccctt tatgcacgtg atcccaaaag ccacacgccg tcgagcaagg
     1681 gcgtgccggt gcggctgtca cgcgcaaaag cgcatacaaa caaagccgct gcgctttccc
     1741 tcgcagaggc gacttgagcc atgagcgccg atatcctctc gcgactgcag ttcgccttca
     1801 cgatcggcta tcatatcctg tggccgacgt ttacgatcgg cacgtccgcg ttcgtcgcct
     1861 ggctcagcat tctcgtttgg ctgacggaca aaccggttta ccgcgacctc ctgcgattct
     1921 ggaggcgttt attcgcgctc ggtttcggaa tgggcgtcat cactgggatc gtgctcagtt
     1981 acgagatcgg caccaattgg gcgggctttt cccgcgcggt tagcaatgtc gtcggtcctc
     2041 tgctgatgta cgaggccgcg accgcctttt tcctcgagtc cggtttcatc gggatcatgc
     2101 ttttcggtga gggacgtgtc tcccgcgggc tgtatgtttt cgcctgcgtg atggtgagtc
     2161 ttgggtcatt aataagcgcc acctggatca tcgcggccaa ctcgtggatg cagacgccat
     2221 caggtgccgt ggccgactcg catggcatat tccacgtcgt cagctggtgg catgtcatct
     2281 tcaacccgtc tttcccgtat cgcttgatgc atatggtctg cgcgagcttt ctcacctgtt
     2341 ccttcgtggt cgccggcgta tcggcttttc atcttctgca gcggcggcat gtcgatgcgg
     2401 cgcgaacggc cctttcgatg gcgctgtggg ctgccctcgt gttggctccg ctgcaaattc
     2461 tgcttggcga tatgcagggc ctcaacacaa ggcaatatca accgacgaag ctcgccgcga
     2521 tggaggggct ctggatcacc gagaaagatg ttcccgctgc ggtctttgct tggccggaca
     2581 tgaagtccga aaagaacctc tacgagatca gtatcccgca tctcgcgagc gtttatctca
     2641 cccatacctg ggacggcgag gtacagggac tgaaggccgt accgccccgc gatcggcctt
     2701 atgtgccgat cgtatttttc gccttccgca tcatggtcgg cgttgggctg atcctgcttg
     2761 ggcttgccgt aacgggcgcg gtactccgct ggcgtggccg tctctttgaa acgaaatggt
     2821 ttcaatatct cgcggttcta gcgacgccgc ttggcttcgt ttcggtcctt gctggctgga
     2881 ctgtcactga aaccggtcgg caaccgtttg tcgtctatgg gatgctccga accgcggatg
     2941 ccgcgtcgcc tgtcgctgct ggcgcgatca cgacgacgct cgtaggcttc ttcgtgattt
     3001 ataatatttt gctgcttggg tttctctatt tcgccgggcg tctggttctc aaaggaccga
     3061 cctggggccc atcctatagt ggcacctttc ggccgggtct cgatcgcgcc gccccgactg
     3121 tcttcggcgc cccagcggcg acgccgacga acgtcggcaa cctttctccc ggagaataag
     3181 atcgatgccc ctgttctttg cgcttctcgc ggccttttcc gtcgctctct atgtgctggc
     3241 ggacggtttt gaccttggcg tcggcatcct gttcctcttt gcgccccggg atatcgatcg
     3301 cgacaggatg atcgaaagca tcgagcccgt ctgggacggc aacgaaacct ggctcgtctt
     3361 cggcggcaca ttgctgtggg ccgcatttcc ggcggcttat ttcgtgctgc taccggcgtt
     3421 ttatctgccc atcctcttca tgctgtttgc actcatattc cgtggcgtcg ccttctcgtt
     3481 tcgcgctgaa agcggacgct tccgccgcgt ttgggatttt gcattcgccg gcggttcgct
     3541 tctagccgcc ttttgccaag gcctggtgct tggcggattc atcggaggcg tgagcatgtc
     3601 aaacggcatg ttcagcggag gcccgttttc ttttttgagc attcttggcg ttctctgcgg
     3661 catcggcctc atcggtggct atgcgctcat gggcgcggga tggctgatct ggaaaacaga
     3721 agggtcgaca caaatcttcg cacgggaaat cgggcatgcg gcgttgattc tgacgacggc
     3781 gttgatggcg atcgtgagcg catggacggc cttcagccaa ccggctgtcg atcagcgctg
     3841 gttctcatgg ccgagtatcg ccgagcttgg cctcgtccca ttggtcacga tagggactct
     3901 cgtcgcaaca tggcgcgcgt tgtgggggac gagcgacacg agggttttcg tattgtccat
     3961 ctttacgttc ttgcttgggt ttctcggtct tctcgtgagc ctttggccct atgtcgtccc
     4021 gcgccacata acaatctggg acagcgcgtc ggatccccag actctttcat tcatcggcgc
     4081 cgggcttatc gtcatcatcc ccattgtgct ggcctaccaa ttccacgctt actgggtctt
     4141 ccggggcaag attccagacc atgcgatagc agatgacggt atcgagctaa atcctgggat
     4201 tcatgcgcgt cacacttgca cgcaggataa cgtccttcat ctcagttgat gtggtgaccg
     4261 tcaggctctt gagtttggcg gatgtttcgc tggcgcgtag cgagccgtcg gcctgaggac
     4321 tgtctcgtgg ccccgaccta catggcttcg ccttcgaccg ctctgcattt gatataatcg
     4381 ccaacattgg gcaaacgagc ctagcatctt caccgcggcc cgctagtaac ggcaatggcc
     4441 atggcgtgcc gccagcagta gcgatggtga tgatagccag tcctagcgcc cggttcgcgg
     4501 actcgacccc gcgccgatgg cgcctcccac gatcgctccc acaggacccg tcgcgcgatt
     4561 acctctatgc gtgccttcct cggaacccaa aaaagctgag ttgcccggtg tcggtatgtt
     4621 aataccagtt aaccacggcg caaaatattt gatggaactg ttcaattttg ctcagaactc
     4681 actgatcggc tggcgagata ttcgtgcctg tgctctgcgg acctagcacc gcgctatagc
     4741 gccgaccacg tcgattctgc gaatatcttc ctccgcagtg tcgataaact ttgaagccgc
     4801 cacatgagcg gcttcctttt tgagaggctg tcaacattag ggagcattaa gatgactgtc
     4861 gaagatcttc cgcaatatcc gcaatggaaa cgcgcgctcc aaaatatgat cgaggcttgg
     4921 gaatcataca aagttatgag tcatctattc gaccagcatc cggaggcgct ccggcggaag
     4981 tcgaactttg agatggcaaa ggctcaatat ttaagagttg cctatgagct agagatgccc
     5041 ggctatctgg ttgtttccgc cagggacgta gattttcggc acgcctgatt ccgatctcgg
     5101 ccgtcagatc atcgttctgc ctgatccctc attccgcgcg aacgacgatc ggcaagggct
     5161 agggcagcgg aacttagccg ccgcattaac gttcgcgtcg gcgacgtctg actccatagg
     5221 aaaaccgcat gttcgaagtc atttcggcta attccattat cgtctggttt gtatggggat
     5281 tttttatggc tttgggttgg gccctcggca catgggtgat gacctggatt cttggctatg
     5341 tacctctttc acggcggcgc tgaatgcgat ggcgactcgt gagggacatt gtggggagtt
     5401 taatgttacg gtacggtgac gttcgtggaa ggctgcgaag ctcgtccatc taaccatgca
     5461 ataacgcctt caacggtctc gccatagaaa acctcgtatt cactccgcga cacgtaaccg
     5521 atatgcggcg tcgctagaac attgtcgaga actctgaaag ggtgtttcgt cggcagaggc
     5581 tcaatatcga aaacatcgat cgccgcgccc gcgagcttac gcccgctcaa tgccgtaacc
     5641 agagcatgtt catccacgat cggcccgcga gacgtgttga ccagccaggc tgttggcttc
     5701 atctgagaaa gttcgcgcgc gcccaccaaa cctcgcgtcg cgtcggcaag cacgaggtgg
     5761 atgctgacga tatcggcatc cctaaagagt tcatctttgg agacgaggcg cgcgcccacc
     5821 tcgtcagctc tctccttggt caggcgttcg ctccaggcaa ttgtctccat gccaaaagcg
     5881 cgcccaatgc gtgcgacctc gcttccgata ttgccaaggc ccaagat
//
LOCUS       DASWOG010000376         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_100713,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000376 DASWOG010000000
VERSION     DASWOG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100713"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..748
                     /gene="hpaR"
                     /locus_tag="VGB93_07820"
     CDS             209..748
                     /gene="hpaR"
                     /locus_tag="VGB93_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEY0146045.1"
                     /translation="MARKKSAGNTESKPAPKAGARRVPMREFSRSLPMSLLRAREAVM
                     RHFRPSLRDHGLTEQQWRILRALTSTGAIEVTELARVAFLLGPSLSRILRDLEARHLI
                     DRKVVKADQRRVVVSISARGLRLIEAVAPSSEAIYAAMTARYGARELARLQEMLQSLE
                     SCLSELRTGDDGDTEANAS"
     gene            921..>1635
                     /locus_tag="VGB93_07825"
     CDS             921..>1635
                     /locus_tag="VGB93_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146046.1"
                     /translation="MKLTRRDFTAGLVAGITAPYVIGSARAQGATIKIGMCVPVTGPA
                     AEQGLYAQNGAKLALAAVNKAGGILGKQAELVIEDDQTTNPGIVLAFSKLAAQPDIVG
                     FLGSIRSTQVHAMAPDVLKLGKPVMIGGTDPNLTHMGNQWLFRFRPNDSYSGRVIADF
                     GVNTLGKKKWAIVHSTDAFGTAGGKALSSALEKLGTPALLDQGYANQSQDFTPVVLAV
                     KQSGADVLGSYFTFENDLGI"
BASE COUNT          342 a          475 c          494 g          324 t
ORIGIN      
        1 atatcgacgc gggcgtcgag attgccggaa tattcgaggg tgaaatgcgg catgtcggga
       61 tcactccaac gattctgcag agccggcgaa aactgatgcg gaaatttgat taacatgtta
      121 aatgcatcgg ttcaaaccga attgcgcctt gctgcattgc aaaacttctc gcgccacgcg
      181 cgtttcccgg aattcacgga ccatcgacat ggcgcggaaa aaatcggcgg gtaatacgga
      241 atcgaaaccc gcacccaagg ccggtgcccg gcgcgtgccg atgcgcgaat tttcccgctc
      301 attgccaatg tcgctgttgc gggcgcgcga agccgtgatg cggcatttcc gtccctcgct
      361 gcgcgatcac ggtttgacgg agcagcaatg gcggattttg cgtgcgctga catcgaccgg
      421 cgccattgaa gtgacggaac tcgcccgtgt tgcctttctg ctgggtccaa gcctgtcgcg
      481 aatccttcgc gatctggaag cgcgccattt gatcgatcgc aaggtggtga aagccgatca
      541 gcgccgggtc gtcgtgtcga tttccgcacg gggtttgcgg ttgattgagg ccgtcgcgcc
      601 gtcatcggaa gcgatctacg ccgccatgac cgcacgctat ggcgcgcgcg aactcgccag
      661 gctgcaggag atgctgcaat cgcttgagag ctgcctgtcg gaattgcgca ccggcgacga
      721 tggagatacc gaggccaacg cgtcgtagag gaccaagacc catgcttatt gcttaggcat
      781 tctcgcggct gcaaaaaatg agatggccag agaccaatcg acgcttgccg cgacatgcca
      841 tggacatgtg agcggttttt cgctcaaact cacgaactta aaaaagccga cgctaaaagt
      901 tggctccagg ggaggaatgt atgaagctca cgcgacgcga cttcacggcc ggattagttg
      961 ccggtattac tgcaccttat gtgatcggca gcgcccgggc ccagggcgcc accatcaaga
     1021 tcggcatgtg cgtgccggtc accggtcccg cggccgagca gggcctttac gcgcagaacg
     1081 gcgcgaagct ggcgcttgcc gcggtcaaca aggccggcgg catcctcggc aagcaggccg
     1141 aattggtgat cgaggacgat cagaccacca atccgggcat cgtgctggcg ttttccaagc
     1201 tggccgcgca accggatatc gtcggatttc tgggatcgat ccgctcgacc caggtgcatg
     1261 cgatggcgcc ggacgtgctc aagctcggca agccggtcat gatcggcgga accgatccca
     1321 acctcaccca tatgggcaat caatggctgt ttcgtttccg tcccaatgac agctattccg
     1381 gccgcgtgat cgccgacttc ggcgtcaaca cgctcggcaa gaaaaaatgg gcaatcgtgc
     1441 attcgaccga tgcgttcgga accgcaggcg gcaaggcgct gagttccgcg ctggaaaaac
     1501 tcggcacgcc cgcattgctc gatcagggtt atgccaacca gagccaggat ttcacccctg
     1561 tcgtgctggc ggtgaagcag tccggcgccg atgttctcgg cagttatttc acgtttgaaa
     1621 atgacctcgg cattt
//
LOCUS       DASWOG010000377         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_118707,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000377 DASWOG010000000
VERSION     DASWOG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118707"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VGB93_07830"
     CDS             complement(<1..764)
                     /locus_tag="VGB93_07830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0146047.1"
                     /translation="MTRIMFFWENFGPTHVDRCDAVARAMPRNDILGIELNPTSSTYT
                     WVPETGQHFTKVTLRDANSHWGALRTAIELVRVSVSSGARHVFLCHYNEPSVQIAAML
                     MRMIGKRVYLLLDSKFDDRPRSIWREALKAIWIWPYVGAIVGSRRSRQYLSFLGLNDA
                     RIENYYDAISVERIRRAAGVPPAPEGVDFHDRHFTIVSRLVAKKNLFVAVDAFALYVS
                     RTNHPRRLRIFGDGGLDRELREHVRELNLTEYVTFEG"
     gene            complement(789..2000)
                     /locus_tag="VGB93_07835"
     CDS             complement(789..2000)
                     /locus_tag="VGB93_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0146048.1"
                     /translation="MRILLNDYPGHAFTVELARELAKRGHNVLHLSLGDFQSPKGDLA
                     HRPDDSATFEVETVSLGLPFKKYDFVARRRQEIDYGHRVIARIASWRPDVLIGCGNPL
                     DPQSMIQNECRQRNICFVFWLQDIYSNAIKTVLRKKIPVVGHAIGIWYEELEKRLLRR
                     SDRIIAITEDFIALLAKWHVPRERISVIENWAPKDKILLLPRDNSWSQARGLNGKFVL
                     MYSGTLGLKHNPSLLIATAEAFKAEPEVQVVLVSEGKYADEVRDTAKTHGLGNLTVLP
                     FQNFENYSAVLATGDVMLAMIEPDAAIYSVPSKVLSYLCGGKAIVLSANAANLAARIL
                     KRSGAGAVVAPDDEAGFVAAIRHFLEDPDARQAAGAGARAYADENFDIGAIAARFETI
                     LAPCAKPPVPA"
BASE COUNT          491 a          622 c          606 g          439 t
ORIGIN      
        1 ccttcgaagg tgacatattc ggtcaggttc aattcgcgca catgttcgcg caattcccga
       61 tcgaggccgc cgtctccgaa aatccgcaac cggcgcgggt gattggtccg cgacacatac
      121 agcgcgaaag cgtcgaccgc cacaaacaaa ttcttcttgg cgacaagccg ggaaacgatg
      181 gtgaaatgcc ggtcgtggaa gtcgacgccc tccggcgcgg gcggcacacc ggcggcgcgg
      241 cgaatgcgct cgaccgagat cgcgtcatag taattctcga tccgagcatc gttcagcccg
      301 aggaagctca gatattgccg gctgcggcgc gagccaacga tggcgccgac atagggccag
      361 atccaaatcg ccttcagcgc ctcgcgccag atcgagcgcg gccggtcgtc gaatttggaa
      421 tcgagcagca aatagacgcg tttaccgatc atgcgcatca gcatggcggc aatctgtacc
      481 gatggctcat tataatggca gaggaaaacg tgccgtgcgc ccgagcttac gctgacgcgg
      541 accagttcga tcgccgtccg cagcgccccc caatgactgt tggcgtcgcg cagggtgact
      601 ttggtgaaat gctgcccagt ctccggcacc caggtgtagg tcgagcttgt cggattcagt
      661 tcgatgccga gaatgtcgtt gcgcggcatg gcgcgcgcca cggcatcgca gcggtcgacg
      721 tgggtcggcc cgaaattctc ccagaagaac ataatgcggg tcacaggttt tctcggtctg
      781 tcgtggcctt aagcaggcac cggcggtttg gcgcaaggcg ccagaatggt ttcaaagcgc
      841 gcagcgatcg cgccgatgtc gaaattttca tccgcatagg cacgcgcacc agcgcccgca
      901 gcctgccgtg cgtccggatc ttccaaaaaa tgccggatcg ctgcgacgaa gccagcttcg
      961 tcgtccggtg caacaacggc gccagcgccg gaacgtttca aaattcgtgc cgcgagattg
     1021 gcggcattgg cggaaagtac aatggccttg ccgccgcaaa gataggacag gactttcgac
     1081 ggcacggaat aaatcgccgc gtccggttcg atcatcgcca gcatcacgtc gcccgttgcc
     1141 agaacggcgc tgtaattctc gaaattctga aacggcaaga ccgtcagatt gccgagacca
     1201 tgtgttttcg ctgtgtcccg cacctcgtcc gcatacttgc cttccgagac gagtacgact
     1261 tgaacctctg gctcagcctt aaaggcttct gccgtcgcaa taagcagcga cggattatgt
     1321 ttgaggccaa gagtcccaga atacatcaaa acaaattttc cattcagtcc gcgcgcctgg
     1381 ctccaggaat tatcgcgcgg cagaagcaag atcttatctt ttggtgccca gttctcgatg
     1441 accgagatgc gctcgcgcgg cacatgccat ttcgcaagca gggcgatgaa atcttccgta
     1501 attgcaataa ttcggtccga acgccgtaaa agtcgctttt caagttcttc gtaccagatg
     1561 ccgatggcat ggccaacaac tggaattttc ttccgcaaaa cagttttaat ggcgttcgaa
     1621 tagatgtcct gcaaccaaaa tacaaaacaa atattacgct ggcggcattc attttgaatc
     1681 atgctttgcg gatcgagcgg attgccgcag ccaatgagga catccgggcg ccagcttgca
     1741 atgcgcgcga tgacgcgatg gccatagtcg atttcctgcc gacgacgcgc gacgaaatca
     1801 tactttttga agggcaggcc gagactgaca gtctcgacct cgaacgtcgc ggaatcgtcg
     1861 ggccggtgcg caagatcacc cttcgggctt tgaaaatcac caagcgaaag atgcaaaaca
     1921 ttatggccgc gcttcgccaa ttcacgcgcg agttcgaccg tgaaggcgtg gccgggataa
     1981 tcgttcagta aaattctcat tcccgctttt gtcctttcct cgcaataaag ccgagccaat
     2041 acgggaattt tggccaattc aaagcggtac gccgcggcaa cgtttatggt gcagcggcat
     2101 gaacctcggc tgtatagccg ccgggaaact caaagaagga tgtgcgccgg cctgctgc
//
LOCUS       DASWOG010000378         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_155190,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000378 DASWOG010000000
VERSION     DASWOG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155190"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /gene="mutS"
                     /locus_tag="VGB93_07840"
     CDS             complement(<1..2191)
                     /gene="mutS"
                     /locus_tag="VGB93_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0146049.1"
                     /translation="MQHRAIIDPEPVGKITPMMVQFIEIKAANPDCLLFYRMGDFYEM
                     FFEDAEVASRALGIVLTHRGKHLGRDIPMCGVPIHRSDEYLARLIALGHRVAVCEQTE
                     DPAEARKRGNQSVVRRAVTRLVTPGTITEESLLDPARANCLLAIMGLRGANATFGIAS
                     VDISTGAFAVTEVAAPALSAEIARLDASEIVAPEVLCGDADFSRLVTDMRIPLSPVGG
                     PHDDPAAAARRLQDYFGLATLDGLGNFSPRERLAAALAIFYVERTQRSARPALSLPSR
                     MRFDAHMEIDAATRANLELTRTLSGHRDGALLEAIDRTVTPGGGRLLAERLAGPLTDV
                     DAIQRRLDAVAFLHDDRGLRDDLRVCLKSAPDLPRPLSRLALQRSGPRDLAAIRDALQ
                     AAETISARLRATKGLPDDLREVAENASRADPAIAAHLIDTLADDLPLKARDGNFIRRG
                     YDAELDELRGLRDESRRIIAELQARYAALAETKQLKLKHNNFLGFFIEVSQAQGEKLL
                     KPPFNATFVHRQTMTDAMRFSTNELVEIEAKMSSAADRALARELGIFDKLSTQILIAE
                     AALKAVAEALAALDVAAALADLAEIRSWTRPEIDISLNFVIEAGRHPVVEAALAREGK
                     PFVANDCNLSGSGSAPGLIAVITGPNMAGKSTYLRQNALIAALAQMGSFVPAKHCKIG
                     IVDRLFSRVGAADDLARGRSTFMVEMVETAAILNQATERSLVILDEIA"
     gene            complement(2262..>2837)
                     /locus_tag="VGB93_07845"
     CDS             complement(2262..>2837)
                     /locus_tag="VGB93_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0146050.1"
                     /translation="CSMLAMWTFVRGNALLAEKMQRDSIPLKELIPMLEKSKPTRVPG
                     TAIFLTNQLTSAPSALLHNIKHNKVLHEHVWLMSVRTEDTPRVPAANRYEIHKLSPDF
                     TTVTVHFGYMESPRIPAALALLRKSGHKFDIMTTSFFLGRRTIKASPNSGMPGWQDNL
                     FIALSRQAIDATEFFSIPSDRVVELGAQVTI"
BASE COUNT          558 a          812 c          935 g          532 t
ORIGIN      
        1 gcgcgatctc gtcgagaatg acgagcgagc gttctgtcgc ctgattgagg atcgcggctg
       61 tctcgaccat ttcgaccatg aacgtcgaac ggccacgggc gagatcgtcc gccgcgccga
      121 cacgcgaaaa gagacgatcg acgataccga tcttgcaatg tttggccgga acgaacgagc
      181 ccatctgcgc cagggccgcg atcagcgcgt tctggcgcag ataggtggat ttgccggcca
      241 tgttcgggcc ggtgatcacg gcgatgagac ccggtgcgct gcctgagccc gagagattgc
      301 aatcattggc gacgaagggc ttgccctcgc gcgcgagcgc agcttcgacg acggggtggc
      361 gtccggcctc aatgacgaag ttaagcgaaa tatcgatctc cggccgcgtc cagctacgga
      421 tttcggcgag atcggccagc gctgccgcga catcgagcgc ggctagcgcc tcggccacgg
      481 ctttgagcgc ggcttcggcg atgaggattt gcgtactcag cttgtcgaat atgcccagtt
      541 cacgcgccag cgcccgatcg gcggcggatg acattttggc ttcgatttcg acaagctcat
      601 ttgtcgaaaa gcgcatcgca tcggtcatcg tctggcgatg cacgaaggtg gcattgaacg
      661 gcggcttgag aagcttctcg ccttgcgctt gcgaaacttc gatgaagaag ccgagaaaat
      721 tattgtgttt gagtttgagc tgtttggttt ccgccagcgc cgcatagcgc gcctgcaatt
      781 cggcgatgat gcggcggctt tcgtcacgta agccgcgcaa ttcgtcgagt tcggcatcgt
      841 aaccgcgccg gatgaaattg ccgtcgcggg ctttgagcgg caagtcgtcg gcaagtgtgt
      901 cgatgagatg cgccgcgatc gcgggatcgg cgcgcgatgc attttcggca acttcgcgta
      961 gatcgtcggg caaacctttg gtcgcacgca gacgcgccga aatagtctcg gcggcctgca
     1021 gagcgtcgcg gatggcagcg agatcacgcg gaccgctgcg ttgcagggcg agccgcgaca
     1081 gcggccgcgg cagatcgggc gcggatttca ggcagacgcg cagatcgtca cgcaggccgc
     1141 ggtcgtcgtg aagaaatgcc acggcatcga gcctgcgctg gatggcatcg acatccgtga
     1201 gaggtcccgc cagccgctcg gcgagaagcc ggccgccacc cggcgtcacg gtccggtcga
     1261 tggcttcaag caacgcgcca tcgcgatggc cggacagcgt tcgcgtcaat tcgagattgg
     1321 cgcgggtggc ggcgtcgatc tccatatgcg catcgaagcg catccggctc ggcagactca
     1381 gagcggggcg ggcgctgcgc tgcgtgcgtt cgacgtagaa aatcgccagc gccgcggcta
     1441 gcctctctct cggtgaaaaa ttgccgagac catcaagcgt cgcgaggccg aaataatcct
     1501 gcaaccgccg ggccgcggcg gcgggatcgt catgcgggcc gccaacaggc gaaagcggga
     1561 tacgcatatc ggtgacgaga cgcgagaaat cggcgtcccc gcaaagcacc tccggcgcga
     1621 cgatttccga tgcatcgaga cgcgcgattt cagcgctgag ggctggggcg gcgacttccg
     1681 tcaccgcgaa tgcgcccgtc gaaatatcga cgctggcgat gccgaacgtt gcgttcgctc
     1741 ctcgcaatcc catgatcgcg agcaggcaat tggcccgcgc cggatcgagc aggctctcct
     1801 cggtaatggt accgggcgtg acgagacggg tgacggcacg gcgaacgact gactggttgc
     1861 cgcgcttcct ggcttcggcg gggtcctctg tctgctcgca gacggcgacc cgatgaccga
     1921 gcgcgatcag acgggcaaga tattcgtcgg aacggtggat gggaacgccg cacatcggaa
     1981 tgtcgcggcc aagatgtttt ccgcgatgcg tcagcacgat gccgagcgcg cgcgaagcga
     2041 cctcggcatc ctcgaagaac atctcgtaga aatcgcccat ccggtagaac agcaggcaat
     2101 ccggattggc ggccttgatc tcgataaatt gcaccatcat cggcgtgatc ttgccgacgg
     2161 gttctggatc gatgatggcg cggtgttgca tcagccgagc ttagcaaagc gcgggcgtcg
     2221 atgcacgggc gcgtccgaga tatgcagagc ttagcgcagc gtcagatcgt gacttgcgcg
     2281 cccaattcga cgacgcggtc cgagggaatc gagaagaatt cggtcgcgtc gattgcctgc
     2341 cgggagaggg cgatgaaaag attgtcctgc cagcccggca tgccggaatt gggcgaggcc
     2401 ttgatggtgc gccgcccaag gaaaaacgat gtcgtcatga tatcgaattt gtggccggat
     2461 ttgcgcagca gcgccagagc ggccggaata cgcggacttt ccatatagcc gaaatgcaca
     2521 gtgacagtgg tgaagtccgg cgacaattta tggatttcgt agcggttggc ggctggcacg
     2581 cgcggcgtat cttccgtgcg taccgacatg agccagacat gctcatgcag aactttattg
     2641 tgcttgatat tgtgcaggag tgctgaaggc gctgaggtta gctgattggt caggaagatc
     2701 gcggttcccg gcacgcgcgt cggtttcgat ttttccagca tcggaatcaa ttccttcagc
     2761 gggatcgaat cgcgttgcat cttttcagcg agcaatgcat tgccgcgtac gaaagtccac
     2821 atcgcgagca tcgaaca
//
LOCUS       DASWOG010000379         6161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_229046,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000379 DASWOG010000000
VERSION     DASWOG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229046"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="VGB93_07850"
     CDS             <1..92
                     /locus_tag="VGB93_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HEY0146051.1"
                     /translation="PTSTGTHLRMEQVGFRPDQEQAYQGAKFG"
     gene            313..897
                     /locus_tag="VGB93_07855"
     CDS             313..897
                     /locus_tag="VGB93_07855"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEY0146052.1"
                     /translation="MGSTIPIEEIIGSGGGETKGTQRLRRALHEHGWRKHKFEVQRTI
                     DGVARESISHEVDHIARFAAGLLALEIEWNNKDPFFDRDLENYKRLHADGAISLGILV
                     TRGVSLQDEMRSMVLRFATEKNINEIADLERIGLNPTIRQRKDYARRQHGEITFAQAW
                     ATSFVQDKFGAATTHWRKLEDRVRRGVGNHVRCF"
     gene            complement(975..1631)
                     /locus_tag="VGB93_07860"
     CDS             complement(975..1631)
                     /locus_tag="VGB93_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEY0146053.1"
                     /translation="MLAKIEPPDPSSELLTLTNGKRFKTIMADPPWRLQNSTGKVAPG
                     HKRLNRYGTMTLAEITALPVAGIADDTSHLYLWVPNALLPDGLAVLHAWGFTYKSNVV
                     WHKVRKDGGSDGRGVGFYFRNVTELLLFGVRGKNARTLAPGRSQVNMIQTRKREHSRK
                     PDEQYDLIESCSPGPYMELFGRGVRSGWTVWGNQADDGYRPTWKTYRHHSQASRELID
                     "
     gene            complement(1737..2657)
                     /gene="argC"
                     /locus_tag="VGB93_07865"
     CDS             complement(1737..2657)
                     /gene="argC"
                     /locus_tag="VGB93_07865"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0146054.1"
                     /translation="MSARVFIDGQSGTTGLGIQARLESMSGIELAELPPAERKNPTAK
                     ADILRTVDLAILCLPNEAAEESAALISTLGNSAPRVLDASTAHRIAPGWVYGFPELES
                     GQSDKIRAAQRVANPGCHATGAIALLRPLVDAGLLPADFPVTITSVSGYSGGGKSMIA
                     DFESGRAPRFELYALELEHKHVPEIMAYGGLTRRPIFVPSVGNFAQGMLVSIPLFLDD
                     LPEKPDAMELHAALASHYVDTANIRVLRPDGETRLEPESLNDTNDLELRVFANPARRQ
                     AVLVAKFDNLGKGASGAAIQNLQLMLGLSE"
     gene            complement(2795..3274)
                     /gene="rpsI"
                     /locus_tag="VGB93_07870"
     CDS             complement(2795..3274)
                     /gene="rpsI"
                     /locus_tag="VGB93_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0146055.1"
                     /translation="MAETLSSLQDLAAANVEAQPAAPVYVQKLDKQGRAYATGKRKDA
                     VARVWIKPGSGKVVVNDRALEVYFARPVLRMILQQPLGIAKRNGQYDITVSVAGGGLS
                     GQAGAVRHGLAKALTHYEPDLRPVLKHEGFLTRDSRVVERKKYGKKKARRSFQFSKR"
     gene            complement(3277..3747)
                     /gene="rplM"
                     /locus_tag="VGB93_07875"
     CDS             complement(3277..3747)
                     /gene="rplM"
                     /locus_tag="VGB93_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0146056.1"
                     /translation="MFGNTHSTKTADVQKNWILIDAKGLVVGRLASIIALRLRGKHKA
                     SFTPHIDDGDNIIVINADKVVFTGRKYDDKVYYHYSGFQGGIKERTARFILEGRFPER
                     IIEKAVERMLPRGPLGRKQLGNLRVYKGADHPHVAQSPVTLDVASLNRKNARSA"
     gene            3891..4325
                     /locus_tag="VGB93_07880"
     CDS             3891..4325
                     /locus_tag="VGB93_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0146057.1"
                     /translation="MTSLPSDGKIRDIPQSVRAIALVGLSANPARDSNGGFAFLLRRR
                     YRAVGVNPGLAGQTIHCAPVYARLADVPFAIDMVDIFRNSEAAGGVVDEALALMPLPK
                     VIWMQLDVINEAAAENAHAHGVGVVMDRCPEIEIARLELPKI"
     gene            complement(4322..5389)
                     /locus_tag="VGB93_07885"
     CDS             complement(4322..5389)
                     /locus_tag="VGB93_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0146058.1"
                     /translation="MSLHSAQAGAVPSYRRAVQIWLWIVAALIFAMVVVGGATRMTES
                     GLSITEWHPISGVIPPLSYDAWVAEFDKFKQIPQYRAIHSDLDLNGFKFIFFWEWSHR
                     LLGRAIGFVVALPLIFFWLRGALTRDLKFKLVGLLVLGGLQGFVGWWMVASGLVNRIE
                     VAQERLAAHLLLASILFAATVWVASSLSPKSAIRSEPWQRSFASTIVVVTLLQIFLGG
                     LVAGLRAGRAYNTWPLMDGHFVPNVLMTLEPWWRNFVDNIATVQFQHRVVAYMLFALA
                     IVQAFLAWRAIPASRAARRATHFAMLVGLQAILGIVTLLLVVPLWAGLVHQAFAMLVL
                     LGAVRYLQALEAGKTNALARP"
     gene            5506..5685
                     /locus_tag="VGB93_07890"
     CDS             5506..5685
                     /locus_tag="VGB93_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEY0146059.1"
                     /translation="MGSVLMLAFVLVYALIAMVLAQAPAIRRAPALMQFFSYAILGMA
                     WVLPLMPLIKWMERD"
     gene            complement(5682..>6161)
                     /locus_tag="VGB93_07895"
     CDS             complement(5682..>6161)
                     /locus_tag="VGB93_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0146060.1"
                     /translation="LAVIADLARQAGIDPLSIPEDLCLDWAGIETLAKHELCTIGVHT
                     LTHPRLGKLTEADMRRELGDSRAQIEAHIGKEAAHLAYPVGNPVAAGVREFEAAAELG
                     FKSAVTTRPGMIFPAHDHRRTALPRLSINGTWQSLDYVEILLSGVPFALWNKGKLVA"
BASE COUNT         1290 a         1801 c         1849 g         1221 t
ORIGIN      
        1 cgccgacgag cacggggacg cacctgcgca tggaacaggt cggcttccgg cccgatcagg
       61 aacaggctta ccagggcgcc aaattcggct gacagaagtt ctttgcgaat ttggaacagg
      121 tgttggcgaa gggggattga agaccttcga ggcatgctgc gacaaaggtg acagaaacta
      181 agcttatgcc taccatcgag gtatgtttga atccctccgt gcccacggct tccaggtcga
      241 gtgtcattct cacgccaaag caattctcag cgtcgatttt cctgagttgc caaccaattt
      301 caaactgttc tgttgggttc aacgataccg attgaggaaa ttattgggtc tggcggcggc
      361 gaaaccaaag gaacacaacg actacgccga gcccttcatg aacacggatg gcgcaaacac
      421 aagtttgagg tgcagcggac aatcgacggc gtagcccgtg aatcgatttc gcacgaggta
      481 gatcatatcg ccagatttgc cgccggcttg ctggctctgg aaatcgaatg gaacaacaag
      541 gacccgttct tcgatcgaga cctcgagaac tacaagcggc ttcacgctga cggtgcaatt
      601 tcactaggca ttctcgtcac gcgcggcgtt tctcttcaag acgaaatgcg ttcaatggtt
      661 cttcggtttg ctaccgaaaa gaacatcaac gaaattgctg acctcgaacg tattggccta
      721 aacccaacta ttcgtcagcg taaggattat gctcgtcgcc agcacggaga aattactttt
      781 gcacaggcat gggccacgtc gttcgtgcaa gacaaattcg gggcggcaac gacgcactgg
      841 cgcaaattag aagaccgcgt tcgtcgcggc gtcggcaatc atgtccgctg cttttgatag
      901 ggttgccgcc cggccttgtg acctttggcg aaccccaggc cgtcgtcgaa cgcttaatcg
      961 aagaaggcga accgtcagtc gattagttct ctacttgctt gggaatgatg cctgtaggtc
     1021 ttccaggtcg gccggtagcc gtcatcggcc tggtttcccc agacagtcca cccactgcga
     1081 acgccacgcc caaatagctc catgtacgga ccaggcgaac aggattcgat taggtcgtac
     1141 tgctcatctg gtttacggga atgctcgcgc ttgcgggtct gaatcatgtt gacctgactg
     1201 cggccaggcg caagcgtcct ggcatttttg ccgcgcacgc caaacagcag aagctcagtc
     1261 acgttgcgga aatagaatcc gacgccgcgc ccgtcggagc ctccatcctt gcgcactttg
     1321 tgccaaacta cattcgattt ataagtgaag ccccaagcgt ggaggacggc aagaccgtcg
     1381 ggcagcagtg cattgggaac ccaaaggtat agatgggacg tgtcgtcggc tatgccagcg
     1441 acaggaagcg ccgtgatctc ggcgagtgtc attgtgccgt agcggttgag ccgcttatgt
     1501 cctggagcaa ctttgcccgt cgagttttgg agacgccacg gcgggtcagc catgatcgtc
     1561 ttaaagcgct taccattcgt aagcgtgaga agctcggatg aaggatcagg cggctcgatt
     1621 ttggctaaca cgaatcgcct cctttgcgtc ctttagtttg catcgggaca tctggacact
     1681 caaccctaac ccgtgcccct gcgattggca ccttattcct cggggaaatc cacgccctac
     1741 tcgctcagcc ccagcatcaa ttgcagattc tggatcgcgg cgcctgacgc gcctttgccg
     1801 agattgtcga acttggcgac gagcacggcc tgccgccgcg ctggattggc gaagacgcgc
     1861 aattcgagat cgttggtgtc gttgagcgat tctggctcga ggcgagtctc gccgtccggc
     1921 cgcagcacgc ggatattggc tgtgtcaaca taatgcgagg cgagcgccgc atgaagctcc
     1981 atggcgtcgg gcttctccgg cagatcgtcg agaaacagcg ggatcgagac cagcatacct
     2041 tgcgcgaaat tgccgacgct cggcacgaag atcggccgcc gcgtcagccc accataggcc
     2101 atgatctccg gcacatgctt gtgttcgagt tcgagtgcat aaagctcgaa acgcggcgcg
     2161 cggccggact cgaaatcagc aatcattgac ttgccgcccc cggaatagcc ggagaccgac
     2221 gtgatggtga ccgggaaatc cgccggaagc agaccggcat cgacaagcgg acgcaacagc
     2281 gcaatcgcgc cggtcgcatg gcagccggga ttggcgacgc gctgggcggc ccggatctta
     2341 tcgctctggc ccgattcgag ttcgggaaag ccatagaccc agccgggcgc gatccggtgc
     2401 gccgtcgaag catcgagcac gcgtggcgcg gaattgccaa gcgtggaaat cagcgcagcg
     2461 ctttcctctg ccgcctcgtt cggcaagcag agaatggcga gatcgaccgt gcgcagaata
     2521 tcggccttgg ccgtggggtt tttgcgctcg gcgggcggca gttccgcaag ctcgattccg
     2581 gacatggatt cgagacgggc ctggatgccc aggcccgttg taccggattg accgtcgatg
     2641 aagacgcgtg cgctcatggc cgcagccccg aaattttatc ctaacgcgtt ttcttcacgc
     2701 gaaccggaat ccacttcgct cgaaaacgct ttggacagtt tcatgcggct gccaagtgaa
     2761 tgtttaatgg caaccgcatg aagccgtaag ataatcagcg tttcgagaac tggaagctgc
     2821 gacgggcttt cttcttgccg tatttcttac gctcgacgac gcgggagtcg cgcgtcagga
     2881 agccttcgtg cttcagcacc gggcgcagat cgggctcgta atgggtcagc gccttggcga
     2941 ggccgtggcg caccgcgccc gcctggcccg agaggccgcc gccggcgacg ctcaccgtga
     3001 tgtcatattg cccattccgc ttggcgatgc cgagcggctg ctgcagaatc atccgcagca
     3061 cgggacgggc gaaatagact tcaagcgcgc ggtcgttgac gaccactttg ccggagccgg
     3121 gcttgatcca gacgcgggcg acagcatcct tacgcttgcc ggtggcatag gcgcggccct
     3181 gcttgtcgag cttctgcacg taaaccggcg cggcgggctg cgcctcaaca ttcgctgcgg
     3241 ccaaatcttg cagcgaggaa agggtttccg ccatcttcag gcgctccttg cattcttgcg
     3301 attgaggctg gcgacgtcga gcgtcaccgg actttgtgcg acatgcggat gatctgcgcc
     3361 cttatagaca cgcagattgc cgagctgctt gcggccaagt ggcccgcgcg gcagcatccg
     3421 ttcgacggcc ttctcgatga tccgctccgg aaagcggccc tcgaggatga accgggccgt
     3481 gcgctctttg atgccgccct gaaaaccgga gtagtgataa tagaccttgt cgtcgtactt
     3541 gcggccggtg aagaccacct tgtccgcatt gatgacgatg atgttgtcgc cgtcatcgat
     3601 atgcggcgtg aagctcgctt tgtgcttgcc gcgcagccga agcgcgatga tcgaggcgag
     3661 ccggccgacg acgaggcctt tggcgtcgat caaaatccag tttttctgga catcggccgt
     3721 cttggtcgaa tgggtgttac cgaacataat aaactcgtgt gattgggctt gcgccgaagc
     3781 gggtgcttct acgcggagag gtgcagttcg tcaatgctga taagccaaaa agacacgaaa
     3841 aaacagctca ttccctcatg cggtattata gcaccacaat ggccaccccg atgacctccc
     3901 tgccctcgga cgggaaaatc cgtgacatcc cgcaaagcgt gcgggcgatc gcccttgtcg
     3961 gcctctcggc caatccggcg cgggattcaa atggcggttt cgcttttctg ctgcgccggc
     4021 gttatcgcgc tgttggtgtt aatccgggtc tggccgggca gacgatccat tgcgcgcccg
     4081 tctatgcccg gcttgccgac gtgccgtttg caatcgatat ggtcgatatt ttccgcaatt
     4141 cggaggcggc tggtggcgtc gtggacgagg ctttggcgct catgccgctg ccgaaagtca
     4201 tctggatgca gcttgatgtg atcaacgagg ccgcagccga aaacgcgcac gctcacggcg
     4261 tcggcgtggt gatggaccgc tgccccgaga tcgagatcgc gcggctggag cttccgaaga
     4321 tttaaggtcg cgccagcgcg tttgtcttgc ctgcctccag cgcctgcaaa tagcgcacgg
     4381 cccccaacag gacgagcatg gcgaaggcct gatgcaccag ccctgcccag agcggcacca
     4441 caagcagcaa ggtcacgatc cccagaatcg cctgaagacc aacgagcatt gcaaaatgcg
     4501 tggcgcgccg ggctgctcgg ctcgccggga tcgcccgcca ggccaagaaa gcttgaacga
     4561 tggccagcgc gaatagcatg taggccacca cgcgatgctg gaattgcacc gtcgcgatat
     4621 tgtcgacgaa attgcgccac cagggctcaa gtgtcatcag gacgttcggc acgaagtgcc
     4681 cgtccatcag cggccaggtg ttataggcac gcccggcccg cagaccggcg acaaggccgc
     4741 cgagaaagat ctgcagaagg gtcacgacaa ctatcgtact cgcgaaactg cgctgccacg
     4801 gctcggagcg aatggcgctt ttcggcgaga gcgaggaagc aacccagacc gtcgcggcga
     4861 agaggattga cgccagaagc aggtgagcgg caagacgctc ctgcgccacc tcgatccggt
     4921 tgacgaggcc ggacgcgacc atccaccagc cgacgaagcc ctgaagaccg ccaagtacca
     4981 gcaaaccgac gagtttgaac ttcaagtcgc gtgtgagggc gccgcgcaac cagaagaaaa
     5041 tcaaaggcaa cgccacgaca aagccaatcg cgcggccaag cagccggtgg ctccattccc
     5101 agaagaaaat gaacttgaag ccatttaaat caagatctga gtgaatcgct ctatattgcg
     5161 gaatttgctt gaatttatcg aactcagcga cccaggcatc gtagctcaat ggcgggatca
     5221 cgccggagat cggatgccat tcggtgatgg aaaggcccga ttccgtcatc cgggttgcgc
     5281 caccgaccac gaccatggcg aagatcaggg ctgcaacgat ccaaagccag atctgcacgg
     5341 cgcgacgata agacgggact gcccccgcct gagccgaatg gagggacatc gaaacgcttt
     5401 gggtcatttt ctatccagat cgggcgcgct acactttggc cagagcggtc gccgcttgga
     5461 agtttccgcg tggaggtctc attgcgcaaa agccagcgca agttcttggg cagcgtccta
     5521 atgctcgcct tcgtgcttgt ctatgcgcta attgccatgg tgctggcgca ggcgccggcg
     5581 atccggcgcg ccccggcgct catgcagttc ttttcctatg caatcctggg tatggcctgg
     5641 gtgctgccgc tgatgccgct gatcaaatgg atggagcggg attaagcgac cagctttccc
     5701 ttgttccaca gcgcgaacgg cacgcccgac agcagaatct cgacgtaatc gagcgattgc
     5761 caggtgccgt tgatcgagag ccgcggcagt gctgtgcggc gatgatcgtg ggcggggaag
     5821 atcatgcctg gccgcgtcgt taccgcgctt ttgaaaccaa gttcggcggc ggcttcaaac
     5881 tcacgcactc cggctgcaac tggattgccg accggatatg cgagatgcgc ggcctctttg
     5941 ccgatatgcg cctcgatctg cgcgcggctg tcaccgagtt cgcggcgcat atccgcttct
     6001 gtcaatttgc cgagccgagg atgcgtgagc gtgtgaacgc caatcgtaca aagctcatgt
     6061 ttggcgagcg tctcgatacc tgcccaatca agacaaaggt cttccgggat tgaaagcgga
     6121 tcgataccgg cctgccgcgc gaggtcggcg atcacggcaa g
//
LOCUS       DASWOG010000380         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_210127,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000380 DASWOG010000000
VERSION     DASWOG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210127"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VGB93_07900"
     CDS             <1..896
                     /locus_tag="VGB93_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0146061.1"
                     /translation="HFGRAAEACNVTQPTLSGQIRKLEEELGLAIFERVGHTVRPTAP
                     GGQILDHARRALAAAEDIVSVAASARDPLAGPLHLGVIPTLGPYLMPYALPAAQQGLR
                     ALRLVLVEDLTVRLIELVAAGKLDAAIIASDPETPALQMETLFDEDFWLVLDGDNPLA
                     ARNKITIADIDPQSLLLLTDGHCLRDQALELCGPPRDAEAAIADMRATSLKTLLHMAA
                     AGYGMTLFPALALRQEEPLPGNLVARRLSGTKARRRVRLIFRPSHPRRAAIAALSKLI
                     RASVKDVLEKENEAAFAGVES"
     gene            complement(946..1398)
                     /locus_tag="VGB93_07905"
     CDS             complement(946..1398)
                     /locus_tag="VGB93_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146062.1"
                     /translation="MNLNYAPLLDIDVLADLGALASLDMCGAEMIDLSPLANHPRLSS
                     LDLGGASKIDLEPLTDCQHLSKLNLWGTIGIDISPLARMTSLADAAQARRGHSSGGVS
                     AARSDFGQHEPVAGFGRLSEPRRTVELINYLRQQQGLAPYYPPGYSSV"
     gene            complement(1553..>1819)
                     /locus_tag="VGB93_07910"
     CDS             complement(1553..>1819)
                     /locus_tag="VGB93_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0146063.1"
                     /translation="AQLKNLRVLDISRTMVSDISPIADLQNLENLNLLSTPSRDLSPL
                     KYLRGLKTLQIEATAIKNIDARSDMEGLQASLCWSNEYQRFSAY"
BASE COUNT          402 a          541 c          488 g          388 t
ORIGIN      
        1 gccatttcgg ccgcgccgct gaggcgtgca acgtcactca accaacactc tcggggcaaa
       61 tccgcaaact cgaagaagaa ctcggcctag ccatcttcga gcgtgtcggc catacggtgc
      121 gcccaacagc gccgggcggc caaattctcg atcatgcccg gcgcgctctg gcggcggcgg
      181 aagacatcgt gtcggttgca gcatccgccc gagatccgct ggccggcccg ctgcatctcg
      241 gcgtcatccc gacgctcggc ccctacctca tgccttacgc attgccggcg gcgcagcagg
      301 ggctgcgcgc gctgcgcctc gtccttgtcg aagacttgac cgtccggctc atcgaactcg
      361 tcgcggcggg caaactcgat gcggcgatca tcgcctccga tcccgagacc cccgctctcc
      421 agatggagac gttgttcgac gaggatttct ggctcgtact tgacggggac aatccgctgg
      481 ccgcgcgaaa caagattacg atcgccgaca tcgatccgca atcattgctg cttctgaccg
      541 atggccattg tctgcgcgac caggcgctcg aactctgcgg gccgccgcgc gatgccgaag
      601 ccgcgatcgc cgatatgcgc gcgacgagcc ttaagacgct gctgcatatg gcagccgccg
      661 gctatggcat gacgctgttt ccggcgctcg cgctgcgaca ggaagagccc ctgcccggca
      721 accttgtcgc gcggcggttg agcggcacca aagcgcgccg ccgcgtgcgc cttatcttcc
      781 ggccaagtca cccgcgtcgc gctgcgattg cggcgctgtc aaaattgatc cgtgccagcg
      841 tgaaagacgt gctggaaaaa gagaacgaag cagcctttgc aggtgtcgaa agttaatagt
      901 cccgaaatat caggaactaa cttcgtgtac ctaaatatcg atttgctaca cggaagaata
      961 tcccggtgga taataaggcg ccaagccttg ttgctgccgg aggtagttga tcaactccac
     1021 ggtccggcgc ggctccgata gtcgcccaaa tccggcaacc ggctcatgct gtccgaaatc
     1081 cgaccgcgct gcagatactc cgccgctaga atggcctctc cttgcttgtg ctgcgtcggc
     1141 tagactcgtc attctggcca gaggcgaaat atcaatgccg atcgttcccc agagatttaa
     1201 cttactcaga tgctgacaat ctgtgagggg ttcgagatca attttagagg cgccccccaa
     1261 atcgagacta gataatcgcg gatggttcgc gagcgggctg agatcaatca tctccgcacc
     1321 gcacatgtcc agcgacgcaa gtgcacccaa gtcggcaagc acatcgatat ctaaaagtgg
     1381 cgcgtaattt agattcaatg accgtaattc cctacaattt cgcagcggcg cgatatcgcg
     1441 tacatcggta tttctaagcg ccaagacttt taaagaggtg agtcgttcca ggggtgttaa
     1501 atctgtaatt aaagttgaat atagggacag ggtctccaat ttcctcagac tgctaatagg
     1561 cgctaaatcg ctgatactcg ttgctccagc ataaagatgc ctgaagccct tccatgtcag
     1621 agcgcgcatc aatattcttg attgcagttg cctctatctg cagcgttttc aatcccctca
     1681 aatatttcaa ggggcttagg tcacgagacg gcgtcgacaa caaattcaaa ttttcaagat
     1741 tttgcagatc ggcaatagga gaaatatcac tcaccatagt gcggcttata tcgaggaccc
     1801 tcaagttttt taattgcgc
//
LOCUS       DASWOG010000381         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_229134,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000381 DASWOG010000000
VERSION     DASWOG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229134"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGB93_07915"
     CDS             <1..352
                     /locus_tag="VGB93_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0146064.1"
                     /translation="SRLEEATKELGVPIVVAESTMRQAPGFLFVPLGEIVLRGRSHPT
                     PIYALHGTAARAGVAFADFLERHRAALAAFAVEAPDAAAKIRAAREHPCGEAYATFYA
                     RLGQDIPAFLPRAV"
     gene            442..1332
                     /locus_tag="VGB93_07920"
     CDS             442..1332
                     /locus_tag="VGB93_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146065.1"
                     /translation="MIDKLEFFIAVAREQSFSRAAETCGVTQPTLSAGIKQLEETLGV
                     LLVNRSSRFHGLTPEGERVLEWAKRIVGDSRAMRQEVRALKSGLTGLMRIAVIPTALS
                     MVAALTTPFRAKHPGVRFSVTSATSQEILQGIDDLEIDAGITYLDNEPLGTVRSLPLY
                     TEQYCLLTSIGSPFSDHRSVTWAEVGRIPLCLLTPDMQNRRIVDQLLVSAGNLPEPTL
                     ESNSVIALYAHVKTGQWASIMAEKLVETLSISDPIRSIPIIEPQAVHQIGLVVPRREP
                     MTPLTATLFAEAKKLIAPQF"
     gene            1506..1976
                     /locus_tag="VGB93_07925"
     CDS             1506..1976
                     /locus_tag="VGB93_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY0146066.1"
                     /translation="MPAVYETQRAQEIIDAHLALEGPLLPIFHALLEEFGFIDDAVVP
                     QIAQTLNITRAEVHGVLTFYHDFRRKPAGRHVLKICRAEACQSRNSEAVARYCLADLG
                     VDWHGTTPDGGITVEPVYCLGLCANGPAALFDEEPIAELTADKLVGVIEEAGAA"
     gene            1973..>2574
                     /locus_tag="VGB93_07930"
     CDS             1973..>2574
                     /locus_tag="VGB93_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY0146067.1"
                     /translation="MTRVFVPRDVVALALGANKIAAAIVENAKARGEDVTIVRNGSRG
                     MFFLEPLVEVETPAGRVAYGPVSARDVTSLFDAGFLQGGAHALRIGRPEDHPYLKRQT
                     RLTFARCGITDPVSIEDYAAHEGWLGLKRAIEIGSTAIVEEITKSGLRGRGGAGFPTG
                     IKWKTVAQTSADRKYIICNADEGDSGTFADRMLMEGDPFLL"
BASE COUNT          487 a          831 c          759 g          497 t
ORIGIN      
        1 ctcgcgtctc gaagaggcga cgaaagaatt gggcgtcccg atcgtcgtcg ccgaatcgac
       61 tatgcggcag gcaccgggtt ttcttttcgt cccgctgggc gagatcgtac tgcgcgggcg
      121 tagccacccg acgccgatct acgccctgca tggcactgcg gcgcgcgcag gcgtggcgtt
      181 tgccgacttc ctggaacgcc atagagcggc gctcgcggct ttcgcagtcg aagcgccgga
      241 cgccgcggca aagatccgcg cggcccgtga gcatccctgc ggggaggctt atgcgacctt
      301 ctatgctcgc ctgggccagg acattccggc cttcctgccc cgcgctgtct gaaatgcgtc
      361 ttgcgtcgcc gtccgcaaag gggcaagcct tgacgccgca gtgggtcccc aactgcggtc
      421 cgctttggcc aaagtcgcgc cttgatcgat aagctggaat ttttcatcgc cgtcgcccgc
      481 gagcagagct tcagccgcgc tgccgaaacc tgcggcgtca cccagccgac tctctccgcc
      541 ggcatcaagc aactcgaaga gacgctcggc gtgctgctcg tcaatcgcag ctctcgtttc
      601 catggcctga cgccggaagg cgagcgggta ctggaatggg ccaagcggat cgtcggcgat
      661 tcccgggcga tgcggcagga ggtccgggcg ctcaagagcg gtttgaccgg gctcatgcgg
      721 attgccgtca tccccacggc gctgagcatg gtcgccgcgc tgacgacgcc cttccgcgcc
      781 aaacatccgg gcgtgcgctt tagcgtcacc tcggccacgt cgcaggaaat tctgcaaggc
      841 atcgacgatc tcgagatcga cgccggcatc acctatctcg acaacgagcc gctcggcacc
      901 gtgcgcagcc tgccgctcta caccgagcaa tattgcctgc tgacaagcat cggcagcccg
      961 ttttccgacc atcggagcgt aacctgggcc gaagtcggcc ggattccact ctgtctgctc
     1021 acccccgata tgcagaaccg gcgcattgtc gatcagctcc tcgtcagcgc cggcaacctg
     1081 ccggagccga cgctcgaatc gaactcggtc atcgcccttt atgcgcatgt aaagactggc
     1141 caatgggcga gtatcatggc ggaaaaactg gtcgaaacgc tctcgatctc cgatccgatc
     1201 cgctcaattc cgatcatcga gccgcaagcc gtgcatcaga tcggcctcgt cgtgccgcgg
     1261 cgcgaaccga tgacccctct gacggccacg cttttcgccg aagccaaaaa actgatcgca
     1321 ccacaatttt aatagatatc gtctatagct ccacggaata tctatattga tcccaggagc
     1381 atagcggacc atctttttag ggacgatcta aagtcgggtc taacaggagg attctcctgt
     1441 ataaaatata acacagcttg ccgtcggtga tcacgaataa tcgccgatta gcgtaggtgg
     1501 gaaacatgcc agctgtctat gagacacagc gggcccagga aatcatcgac gcgcatctcg
     1561 cgctcgaagg gccgctgctg cctatcttcc atgctcttct cgaagagttc ggcttcattg
     1621 acgatgccgt cgtgccgcag atcgcgcaga cgctgaacat tacgcgggcc gaagtccacg
     1681 gcgtcctgac gttctatcat gattttcgcc gcaagccggc gggccgccat gtcctgaaaa
     1741 tctgccgcgc ggaggcctgt caatcgcgca atagcgaggc ggtcgcgcgc tattgtctcg
     1801 ccgacctcgg cgtcgactgg cacggcacga cgccggatgg cgggatcacc gtcgagcctg
     1861 tctattgtct cggcctctgc gccaatggcc cggcggcgct cttcgacgaa gagccgattg
     1921 cggaattgac cgccgacaaa ctggttggcg tcattgaaga agcgggtgca gcgtgacccg
     1981 cgtcttcgtc ccccgcgacg ttgtcgctct ggcactcggt gccaacaaga tcgccgccgc
     2041 aattgttgaa aatgctaaag cacgcggcga ggatgtcacg attgtccgca atggctcgcg
     2101 cggcatgttt tttctggagc cactggtcga agtcgaaacg cctgccggtc gtgtcgccta
     2161 cgggccggtt tcggccaggg atgtaacgtc cctcttcgac gccggtttcc tccaaggcgg
     2221 cgcgcacgcg ttgcgcatcg ggcggccgga agatcatccc tatctcaaac gccaaacgcg
     2281 cctgaccttc gcccgttgcg gcatcacgga tcccgtctcg attgaggatt atgccgccca
     2341 cgagggctgg cttggtttga agcgcgccat tgagattggc agcactgcaa tcgtcgaaga
     2401 gattacgaaa tccgggctgc gcggccgcgg cggcgccggt ttcccgaccg gcatcaaatg
     2461 gaagaccgtg gcgcagactt ccgccgaccg caaatacatc atctgcaatg ccgacgaagg
     2521 cgacagcgga accttcgccg accgcatgct gatggagggc gatcccttcc tgct
//
LOCUS       DASWOG010000382         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_265922,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000382 DASWOG010000000
VERSION     DASWOG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265922"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB93_07935"
     CDS             <1..463
                     /locus_tag="VGB93_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0146068.1"
                     /translation="TDKDAAFPVHPGAAAFVDGEEKSFLDRYSDYIWWGLMLLSATGS
                     AGAWFAGYLKKDERNNNSSLREPLLDMLAAARRSDSIEELDTMQAEADTILRDTLVSF
                     EHGAVEEGALTAFNIALEQFHNAVADRKALLISMPQNLQRAGAQFRATGTL"
     gene            684..>1784
                     /locus_tag="VGB93_07940"
     CDS             684..>1784
                     /locus_tag="VGB93_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0146069.1"
                     /translation="MPIKIPRWTSQSLSRRRFLSTAATAGAGALGTIAMPYLSRAADR
                     PQITHGVQSGDVGADGGVIWARADRPSQMMVEVATTESFANARVLPPIAALPESDFTA
                     KMLVENLPAGQDIFYRVKFRDLSHTDVSSEPAIGRFRTAPADRRNVSFVWGGDVAGQG
                     WGINPDDGGMLTYATMRKHRPDFLLHSGDTIYADGIIPAEVKLPDGKIWKNVTIPEKA
                     KVAETLDEYRAAHKYNFLDDNVRAFNAEVPIFVQWDDHEVTNNWSASKQLPAAYKERD
                     IALLAARAGRAFHEMYPMRESIVEPGRVYRVLNYGPHLDVFMLDERSYRGPNGPNLQT
                     TYGPDAYFLGPDQLSWLKRGLSNSHATWKVIAS"
BASE COUNT          368 a          580 c          510 g          326 t
ORIGIN      
        1 caccgacaag gatgccgcgt tcccggtgca tccgggcgcc gcggcctttg tcgacggcga
       61 ggaaaagagt tttctcgatc gctatagcga ttacatctgg tggggcctga tgctgctgtc
      121 cgcgacaggc tctgccggcg cgtggttcgc aggttatctg aagaaagacg agcgcaacaa
      181 taactcgtca ctgcgcgaac ccctgctgga catgctcgcg gccgcgcgcc gcagcgattc
      241 catcgaagaa ctcgacacca tgcaggccga ggcggacacc atcttgcgcg atacgctggt
      301 gagcttcgag cacggtgcgg tcgaagaagg cgccctgacc gccttcaaca tcgcgctcga
      361 gcaattccac aacgccgtcg ccgaccgcaa ggcgttgctg atcagcatgc cgcaaaatct
      421 gcagcgggca ggcgcccagt tccgcgcgac cggaacgctg tagacagtcc tttcggctat
      481 cggtttctgc aatcgaactg tcacactatt ctcctatagc gttttcgagc gaagtggatg
      541 ccggttcgcg tgaagaaaac gcgtcaaaac aaaagactag agcccggttc tgatccaatc
      601 agaaccgata aggctctagg tctgtctgcg agacttcacg cataagattt catgcattct
      661 gaccatcgat cagggagatc cgcatgccga tcaagattcc ccgctggact tctcagagcc
      721 tttcccgccg gcgttttctt tcgaccgccg ccacggcggg cgccggtgcg ctgggtacca
      781 tcgcgatgcc ctatctcagc cgcgcggctg accggccgca gatcacccat ggcgtacaat
      841 ccggcgatgt cggcgccgac ggcggcgtga tatgggcgcg tgcggaccgc ccctcgcaga
      901 tgatggtcga ggtcgcgacc acagaatcat tcgccaatgc gcgggttctg ccgccgatcg
      961 cagctctgcc cgagagcgat ttcaccgcga aaatgctggt cgaaaatctc cccgccggac
     1021 aggacatttt ctaccgcgtc aaattccgcg acctgtcgca caccgacgtt tccagtgagc
     1081 cggcgatcgg ccgcttccgc acagcgccgg ccgaccggcg caatgtcagt tttgtatggg
     1141 gtggcgacgt cgccgggcaa ggctggggca tcaatcccga cgatggcggc atgctcacct
     1201 acgccaccat gcgcaagcat cggccggatt tcctgcttca ttccggcgac accatctatg
     1261 ccgacggcat catccccgcc gaggtgaagc tgcctgacgg caagatatgg aagaatgtca
     1321 cgatcccgga aaaagcaaag gtcgccgaga cgctcgacga ataccgcgca gcgcacaagt
     1381 acaatttcct cgacgacaat gtccgcgcct tcaatgccga agtcccgatc ttcgtgcagt
     1441 gggacgacca cgaagtcacc aacaactggt cggcgtccaa gcaactcccc gccgcctaca
     1501 aggagcgcga tatcgcgctg ctcgcggcgc gcgccggccg cgcgttccac gagatgtatc
     1561 cgatgcgcga aagcatcgtc gagccggggc gggtctaccg cgtcctcaat tacgggcctc
     1621 atctcgacgt cttcatgctg gatgaacgca gctaccgcgg ccccaatgga cccaatctgc
     1681 aaacaacata cggcccggac gcctattttc tcggacccga tcagctatca tggttgaagc
     1741 gcggcctgtc gaactcgcac gcgacctgga aggtgatcgc atcc
//
LOCUS       DASWOG010000383         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_247256,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000383 DASWOG010000000
VERSION     DASWOG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247256"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGB93_07945"
     CDS             <1..183
                     /locus_tag="VGB93_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0146070.1"
                     /translation="VVTMQDLILYDIDGEDASGRINGKHRSTGIGRPRFWERARYFGE
                     EVRLAAALDASEVREP"
     gene            218..1198
                     /locus_tag="VGB93_07950"
     CDS             218..1198
                     /locus_tag="VGB93_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0146071.1"
                     /translation="MTPMQALLSAFLAAIAVGGFGYVFAYPLLSGERKAEKRQAAYTA
                     ATATRKVSDKNVESSARRKQVADSLKEIERRNARKRVSLDVKISRAGLHISKQVFFIY
                     SGISGLVFGVLFYALSHQIYLAGAAAVAGALGLPHWFLGFRTKRRLKKFVNLFPESID
                     IIVRGVKAGLPLGDCLRIIATEVDEPVRGEFRQVVEAASMGLSISEACERLATQVPIS
                     ETSFFAIVINIQQKAGGNLSEALSNLSGVLRARKMMEGKIKAFSSEAKASAMIIASLP
                     FIVAGLVYITSPAYISLLWEKPSGQLVLAGSAIWMAIGVFIMNKMISFDY"
     gene            1226..>1768
                     /locus_tag="VGB93_07955"
     CDS             1226..>1768
                     /locus_tag="VGB93_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0146072.1"
                     /translation="MDDLIQTLLDRQTILAALVAIATFATILTVTIPLFETDRFSRRM
                     KIIASERERIRARERDRLNESKISLRQQPKAYMKNIVERFNLSSWLGTDKAKKQMIMA
                     GFRGPQAEVAFLFFRLVTPIILFLLTLLYLFFLSNFGWSNIMKIGVAIGALYIGLKAP
                     EIYISNTIAKRQQSMGRAVPN"
BASE COUNT          381 a          508 c          486 g          393 t
ORIGIN      
        1 gtcgtgacca tgcaggacct gatcctctac gacatcgacg gcgaagacgc ctcgggccgc
       61 atcaatggca agcatcgctc gaccggcatc ggccggcctc ggttctggga gcgtgcgcgt
      121 tattttggtg aggaagttcg cctcgccgcg gcgctggacg ccagcgaagt gagggaacca
      181 tgagggtaat gccgtcaatc gcgtcgcgag gctcaaaatg acgccaatgc aagccctcct
      241 ctcggccttc ctcgcggcaa tcgcagtcgg cggcttcggc tatgttttcg cctatccttt
      301 gctgagcggc gaacgcaaag cggagaaacg gcaagctgcc tatacggccg ccaccgccac
      361 gcgaaaggtg agcgacaaga acgtcgaatc ttcggcgcgg cgcaagcagg ttgccgacag
      421 cttaaaggaa atcgagcgca gaaatgcacg caagcgcgtg agcctcgacg tcaagatctc
      481 gcgcgcgggt ctgcacattt caaaacaggt attcttcatc tattctggaa tttcggggct
      541 ggtgttcggc gtcttgtttt atgctttgag ccaccagatc tatcttgctg gcgccgccgc
      601 tgttgccggc gccttaggat tacctcattg gttcctcgga tttcgaacca aacggcgttt
      661 gaaaaaattc gtcaatctct tcccggaatc gatcgatatc attgtccgcg gcgtcaaggc
      721 cggcttgccg cttggcgatt gcctgcgcat catcgcgacg gaagttgacg aaccagttcg
      781 cggcgaattt cgccaggtcg ttgaggcagc atcgatggga ctttcgatct ccgaagcctg
      841 cgagcggctg gcaacacaag taccgatctc tgagacgagc tttttcgcga tcgtcatcaa
      901 cattcagcag aaggcgggcg gaaatctttc cgaagcttta tcgaaccttt ccggcgtgct
      961 gcgcgctcgc aaaatgatgg agggcaagat caaggccttc tcgtctgagg caaaagcctc
     1021 ggcaatgatc attgcgtcgc ttccgttcat cgtcgccggc ttggtctata tcacgagccc
     1081 tgcctacatt tcgcttcttt gggaaaagcc gagcggtcag ctcgttctcg cggggagcgc
     1141 gatctggatg gcgatcggcg tattcatcat gaacaagatg atcagcttcg actactgacg
     1201 ctgggtttct gacaggcgga cggccatgga cgatctgatt caaacactgc tcgaccggca
     1261 aacgatcttg gcggcccttg tcgcaatcgc gacattcgcc acaattctga cagtcactat
     1321 tccgctcttt gagacggacc ggttcagccg acggatgaag atcatcgcct ccgaacgcga
     1381 gcgcatccgt gcccgtgaac gcgaccggtt gaacgaatcg aaaatcagtc tgcgtcaaca
     1441 gccaaaagcc tatatgaaaa acatcgtcga acgcttcaat ctgtcctcat ggcttgggac
     1501 ggataaagcg aagaagcaga tgatcatggc cggcttccgg gggccgcagg ctgaggttgc
     1561 cttcctcttc ttccgccttg tcacacccat catcctgttc ttgctgacgc ttctctatct
     1621 cttcttcctc agcaatttcg gctggtcgaa tattatgaaa atcggcgttg caatcggcgc
     1681 cctgtacatt ggcctgaaag cgccggaaat ctatatcagc aataccatcg ccaaacgaca
     1741 acagtcgatg ggcagggccg taccgaac
//
LOCUS       DASWOG010000384         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_192737,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000384 DASWOG010000000
VERSION     DASWOG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192737"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGB93_07960"
     CDS             complement(<1..261)
                     /locus_tag="VGB93_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0146073.1"
                     /translation="MSDAHAKPAHPYHLVDPSPWPIIGAFGAFLMAVGAVLWMKSIPI
                     DAVKPGGYVFFAGVICVLFVMANWWLDVIKEAQTPGVHTPVVS"
     gene            complement(343..951)
                     /locus_tag="VGB93_07965"
     CDS             complement(343..951)
                     /locus_tag="VGB93_07965"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0146074.1"
                     /translation="MALPSQHPSPRRARGARQGFVAFTVFAVAIGMLGMSFAAVPLYR
                     AFCAATGFAGTTQVRHVAPTVEGKRIMTVHFDANVGRGLALSFAPEILQVSVRTGQTA
                     TVFYKVTNLTDHEIAARAVYNVSPGQTGAYFDKLACFCFTEQHFGPHQTAEMPVVFFL
                     DPALEKDDTMSGIDEVTLSYTFYPAHDGQPLAQATGIAPPRL"
     gene            complement(960..1130)
                     /locus_tag="VGB93_07970"
     CDS             complement(960..1130)
                     /locus_tag="VGB93_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146075.1"
                     /translation="MMKYDLDPKGVTLTPEQAKSRRLRNIAIGLVIGALVVIFYAVTI
                     VKFGPGVTKPPI"
     gene            complement(1127..2062)
                     /locus_tag="VGB93_07975"
     CDS             complement(1127..2062)
                     /locus_tag="VGB93_07975"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0146076.1"
                     /translation="MSVIDDSSASGFVAISTAEPRDYLALVKPRVMQLVVVTALAGIL
                     LAPHPVNFVIGFASLLCIAVGAGASGALNMWYDSDIDAVMRRTSKRPIPMGRITRPET
                     LAFGLILAGFAVGTLCLVANLLAGALLAFTIFFYIVIYTMWLKRSTPQNIVIGGAAGA
                     LPPIVGYAAATGHIAVAPLVLFALIFMWTPPHFWALALARTTDYGKAGVPMMPNVKGA
                     DHTRLEILIYSVILAGVGVLPYALGFVSIVYGIIALGLGVEFLRRAIRVFRVRDGEAA
                     NKTAMGLFWFSIVYLYVLFAEILIERLVFVIGVLR"
     gene            complement(2116..3777)
                     /gene="ctaD"
                     /locus_tag="VGB93_07980"
     CDS             complement(2116..3777)
                     /gene="ctaD"
                     /locus_tag="VGB93_07980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0146077.1"
                     /translation="MSTTATAHDAHGHPTGWRRFLYSTNHKDIGTLYIIFAICAGVFG
                     AALSIAMRAELQEPGLQVFPWLARILDGAPPDMATDYAKNLYNVFITAHGLTMIFFVV
                     MPALIGGFGNWFVPLMIGAPDMAFPRMNNISFWLLPASFTLFFISFFMEGAPGMKGFG
                     GGWVMYPPLSEQGHPGPAMDFVILSIHLAGASSILGAINFITTIFNMRAPGMTLHRMP
                     LFAWSVLVTAFLLVLSLPVLAGAITMLLTDRNFGTSFFKPEGGGDPILFQHLFWFFGH
                     PEVYILILPGFGIVSHVISTFSKKPIFGYLGMAYAMVAIGVVGFVVWAHHMYTVGLSL
                     DTQRYFVFATMVIAVPTGIKVFSWIATMWGGSISFRVPMLWATGFIFLFTVGGVTGVV
                     LANAGIDRYLHETYYVVAHFHYVLSLGAVFAIFAGFYYWFPKMSGYMYNETLGKIHFW
                     MMFIGVNVTFFPQHFLGLAGMPRRYIDYPDAYRLWNEVSSLGAYLSGLSVILFLYILF
                     DAFAKKRLAGDNPWGVGATTLEWTVSSPPPFHSYEVLPRIVGSDH"
     gene            complement(3828..>4287)
                     /locus_tag="VGB93_07985"
     CDS             complement(3828..>4287)
                     /locus_tag="VGB93_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0146078.1"
                     /translation="TVKVTGHQWYWTYSYPGDGFDFDSYITADKDLKPGDLRQLTVDN
                     AAVVPVNKTVLVQVTATDVIHSFTVPSFGVRVDAVPGRMNETWFRATKEGTYYGQCSK
                     LCGEDHAYMPIVFRVVSDADYAAWLVDAKKKFSANALPHYADASPATPRN"
BASE COUNT         1011 a         1250 c         1325 g          701 t
ORIGIN      
        1 cgagacgaca ggcgtatgca cgccgggcgt ttgcgcttcc ttgatgacgt cgagccacca
       61 gttcgccatg acgaacagca cgcagatcac gccggcgaag aaaacatagc cgccgggctt
      121 cacggcgtcg atggggatcg acttcatcca taggacggcg ccgaccgcca tgagaaaggc
      181 gccgaaggcg ccaatgattg gccacgggct cggatcgact agatgatatg gatgggcggg
      241 cttggcatgc gcatcagaca tttcgttccc tctcgtctct ccggggtttg acacccctgc
      301 ggctcccttg tcccgtgccg cgtttcctcg atctatgcgt tatcagagtc gcggtggcgc
      361 gatgcccgtg gcctgggcca aaggctgtcc gtcatgcgcg gggtagaacg tgtaggacag
      421 cgtcacctca tcgatgccgc tcatcgtgtc atctttttcc aacgcggggt cgagaaagaa
      481 gacgacaggc atctcagccg tttgatgcgg accgaaatgc tgctcggtga aacagaagca
      541 ggcgagcttg tcgaaatagg cgccggtctg gccgggcgaa acattataga cagcgcgcgc
      601 cgcgatctcg tgatcagtga gattggtgac cttgtagaac actgttgcgg tctggcccgt
      661 tcgcacgctc acctgcagga tttccggcgc aaacgacagc gcaaggccac gcccgacatt
      721 ggcatcgaaa tgcaccgtca tgatgcgttt accctcgacc gtcggagcga cgtggcgcac
      781 ctgcgtcgtg ccggcgaagc cggtcgcggc acagaaagcg cgatagagcg gcacggccgc
      841 gaacgacatg cccagcatcc cgatcgcaac ggcgaagact gtaaaagcga caaaaccctg
      901 acgcgcgcct cgcgcgcggc gtggtgacgg gtgttgtgag ggaagcgcca tctcggatcc
      961 tatatcggcg gcttggtgac gccggggccg aatttgacga ttgtcacggc atagaaaatc
     1021 acgacgagcg cgccgatgac aagaccgata gcgatgttgc gcaggcggcg gctcttcgcc
     1081 tgctcaggcg tcagggtcac accctttgga tcgagatcgt acttcatcac cgcaataccc
     1141 cgatgacgaa gaccagacgc tcgatcagga tctcggcaaa aaggacatag agatagacga
     1201 tcgagaacca gaaaaggccc atcgcggtct tgttggccgc ttcaccgtcg cgcacgcgga
     1261 aaacccggat cgcgcgccga aggaattcaa cgccgagccc aagagcgata atcccgtaaa
     1321 cgatcgacac gaagccaagc gcataaggca gaacaccgac gccggcgagg atcacgctgt
     1381 agatcaggat ctcgagccgc gtgtggtctg cgcccttgac gttgggcatc atcggcacgc
     1441 cggctttgcc gtagtcggtc gtccgcgcca gcgccaaggc ccagaaatgc ggcggcgtcc
     1501 acatgaagat gagggcgaac aaaacgagcg gtgcgacggc gatatgacca gtcgcggccg
     1561 catagccgac gattggcggc agcgcgccgg ccgcaccacc gatgacaatg ttctgcggcg
     1621 tcgaacgctt gagccacatc gtatagatga cgatgtagaa gaagatcgtg aaggcgagca
     1681 aagcgccggc cagcagattg gcgacgagac acagcgtccc gaccgcgaag cccgcaagga
     1741 tgaggccgaa tgccaatgtc tcgggtcgtg taatgcgccc catcgggatc ggccgtttgc
     1801 tggtacgccg catcaccgcg tcgatatcgg aatcgtacca catgttgagc gcgccggagg
     1861 cgcccgcgcc gacggcaatg cagagaagtg acgcaaatcc gatgacgaaa ttcaccggat
     1921 gcggcgcaag cagaatgccg gcgagcgcag ttacgacaac aagctgcatg acccgcggct
     1981 tgacgagtgc cagataatca cgcggctcgg ccgtggaaat cgccacgaac ccggatgcgc
     2041 tgctatcgtc gataacgctc attttcactc gatgttaatg cggcgggcgg gatgaaactc
     2101 ccgccccgcc tcagatcaat gatcgctacc gacgatgcgc ggcagaacct cgtaggagtg
     2161 gaacggcggc ggcgaactca ccgtccattc aagcgtcgtc gcgcccacac cccacggatt
     2221 gtcgccggca aggcgcttct tcgcgaacgc gtcgaagagg atgtagagga agaggatgac
     2281 gctgaggccc gagagatagg cgcccaacga ggaaacctcg ttccacaaac ggtacgcatc
     2341 gggatagtcg atatagcgac gcggcatgcc ggcgaggccg aggaaatgct gcgggaagaa
     2401 ggtgacgttc acgccgatga acatcatcca gaagtgaatc ttgccgagcg tctcgttgta
     2461 catgtagccg ctcatcttcg ggaaccagta gtagaagccc gcgaagatgg cgaacacggc
     2521 gccaagcgac agcacataat ggaagtgcgc cacgacgtaa taggtctcgt gcagatagcg
     2581 gtcgataccg gcattggcca gcacgacgcc agtcacgccg ccgacggtga agaggaagat
     2641 gaaccccgtc gcccaaagca tcggcacgcg gaacgagatc gacccgcccc acatcgtcgc
     2701 gatccacgag aacaccttga tgccggtcgg caccgcgatc accatcgtcg cgaagacgaa
     2761 atagcgctgc gtgtcgagcg acaggccgac cgtgtacatg tggtgcgccc agacgacgaa
     2821 accgacgacg ccaattgcga ccatggcata agccatgccg agatagccga agatcggctt
     2881 cttcgagaag gtcgagatca catggctgac gatgccgaag ccgggcaaga tcagaatgta
     2941 aacttcggga tggccgaaga accagaagag atgctggaac aggatcggat cgccgccgcc
     3001 ttcgggcttg aagaaggagg tgccgaagtt acggtcggtc agcagcatgg tgatggcacc
     3061 agcgagaacc ggcagcgaca gcaccagcag gaacgccgtg acgagaaccg accaagcgaa
     3121 cagcggcatc cgatgcaggg tcatgcccgg ggcgcgcatg ttgaagatcg tggtgatgaa
     3181 attgatcgca ccaaggatcg aagaagcgcc ggcaagatgg atcgacagaa tgacgaaatc
     3241 catcgccgga ccaggatggc cctgttcgga cagcggcgga tacatgaccc atccaccgcc
     3301 gaaacccttc atgcccggcg cgccctccat gaaaaacgag atgaaaaaga gcgtaaagga
     3361 cgccggcagc agccagaacg aaatattgtt catccgcggg aaggccatat ccggcgcgcc
     3421 gatcatcagc ggaacgaacc aattaccgaa cccaccgatc agcgccggca tgacgacgaa
     3481 gaagatcatc gtcaggccgt gtgcagtaat gaatacgttg tagaggttct tggcgtagtc
     3541 cgtcgccatg tccggcgggg cgccatcgag aatcctcgcc aaccacggga agacctggag
     3601 gcccggttcc tgcagttcgg cccgcatggc aatcgacaag gcggcgccaa agacgccggc
     3661 acagatagcg aatataatat agagcgtgcc gatgtcttta tggttggtcg aataaaggaa
     3721 acgccgccat ccggtgggat ggccatgtgc gtcatgcgcg gtcgcggtcg tgctcatcgt
     3781 tggtccccgg gaatcctcga tattattggc tatattggcg caaaaaccta gttcctcggc
     3841 gtcgcgggcg acgcatcggc gtaatgcggc aaagcattag ccgagaattt cttcttcgcg
     3901 tccaccagcc aggccgcata atcggcatca ctcacgacgc ggaagacgat cggcatatac
     3961 gcatgatctt ccccgcagag cttggagcac tgcccgtagt acgtgccttc cttcgtggcg
     4021 cggaaccaag tctcgttcat gcggccaggc acggcgtcga cgcggacacc gaaggacggc
     4081 actgtgaagg aatgaatgac atcggtcgcg gtgacctgaa cgagaacggt cttgttgacc
     4141 ggcaccacgg cggcgttatc gaccgtgagt tggcgcagat cgcccggttt caggtccttg
     4201 tcggccgtga tgtaggagtc gaaatcgaac ccgtcgcccg gataggagta agtccaatac
     4261 cattgatggc cagtaacctt caccgtc
//
LOCUS       DASWOG010000385         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_155360,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000385 DASWOG010000000
VERSION     DASWOG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155360"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..908
                     /locus_tag="VGB93_07990"
     CDS             159..908
                     /locus_tag="VGB93_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146079.1"
                     /translation="MIVFVTGASAGFGAAIARRFAKEGHRIVVTGRRQERLDALADEL
                     GRDKILPLVFDVRDRDAVQRAFAELPAAFAEIDVLVNNAGLALGLEPAQRADLDEWDA
                     MVDTNVKGLMYVTRAALPGLVARNRGLIVNLGSTAARYPYPGGNVYGATKAFVHQFSL
                     NLRADLIGTRVRVTDIQPGLVGGTEFSAVRFHGDADRVTKLYEGADPLTPEDIAEAVY
                     WVATLPPRVNINAIEVMPVTQASGPLAVHRY"
     gene            complement(965..1657)
                     /locus_tag="VGB93_07995"
     CDS             complement(965..1657)
                     /locus_tag="VGB93_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0146080.1"
                     /translation="MTEVLEKQSELEAVGPAFLKDKMLEMRAKTRATIHAIAAGCKAG
                     MVEEDAVAMAKDLLAEGDMVRGWHDVYVRFGTNTLKTFGAPSDPGIVLKDNDIFLIDI
                     GPVWGEFEGDGGDTFTVGDVPDQARCARDAKKLFHIVRKKWESTGMSGRELYEFATEE
                     AKGMGWELNMDLSGHRLSDFPHAAIYDGPMAEVDFKPSPLLWVLEIHIRHPTAGYGAF
                     FEDMLLDDSYFA"
     gene            complement(1671..2132)
                     /locus_tag="VGB93_08000"
     CDS             complement(1671..2132)
                     /locus_tag="VGB93_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0146081.1"
                     /translation="MFPFKFVAPDVSGPAEIMRLAVYTGPVPFQKCWMTNSDDSLCLL
                     TEERSVLFEFFLDGVAVAAMPGESVLAACQRTGVALATVCKGRGICGACRVRVEEGFT
                     ALPPASAQEVRLIGYLGRGEDTRTHRLACQIAMVESLSGLRLQTHPVVAKS"
     gene            complement(2142..>2328)
                     /locus_tag="VGB93_08005"
     CDS             complement(2142..>2328)
                     /locus_tag="VGB93_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146082.1"
                     /translation="RDGSDEQEAMKHATALLAEMSGTIALARITGSPAKAQDILQQKR
                     IELKTRLGLTDESGAAG"
BASE COUNT          437 a          738 c          689 g          464 t
ORIGIN      
        1 acggcgcttg taaaagcagc cgatcattat aatgcagggc actgcggcga gtcttgttgg
       61 cgcgttgaaa cgacgcgcga gtggcggatc gctccccctc ccctttccgt ctcatcactc
      121 ctaaattagc tccgggtttc cacggagagc aatagaccat gatcgttttc gtcaccggcg
      181 cgagcgcggg attcggggct gcgatagcgc ggcgtttcgc caaggagggc caccgcatcg
      241 tggtgacggg gcgccggcag gagcgtctcg atgccctcgc ggatgaactc ggccgggaca
      301 aaatcctccc gctcgtcttc gacgtccgcg accgtgacgc tgtccagcgg gcattcgcgg
      361 aactcccagc cgccttcgcc gagatcgacg tgctcgtgaa caatgccggg ctcgcgctcg
      421 gtctcgagcc ggcgcaaagg gctgatctcg atgaatggga tgcgatggtc gacaccaacg
      481 tgaaagggtt gatgtatgtg acgcgcgccg ccctgcccgg cctcgtcgcg cgcaatcgcg
      541 gcctgatcgt caacctcggc tctaccgctg cccgctatcc ctatcccggc ggcaacgtct
      601 atggcgccac gaaagccttc gtgcatcaat tctcgctaaa cctgcgcgcg gacctgatcg
      661 gcacgcgcgt gcgcgtgacc gatattcagc cgggccttgt cggcggcacg gaattctcgg
      721 cagtgcgctt ccacggcgat gcggatcgcg taaccaagct ctatgagggc gctgatccgt
      781 tgacgcccga ggacatcgcg gaagcagttt attgggtcgc gacgctgccg ccgcgcgtca
      841 atatcaacgc gatcgaagtg atgcccgtga cgcaagcgtc cggcccgctc gcagtgcatc
      901 gctattagcc aaggcgctgg cggctcggca ggcgctcggg ccgagccgtt tttcgcaaaa
      961 gcgctcaggc gaaatagcta tcgtccaaca acatatcctc gaagaacgcg ccgtagcccg
     1021 ccgtcggatg gcggatatgg atttccaaaa cccacagcag cggcgacggc ttgaaatcga
     1081 cctccgccat tggaccgtcg taaatcgcgg cgtgggggaa atccgagagc cgatggccgg
     1141 agagatccat attgagttcc cagcccatcc ctttggcttc ctcggtcgcg aattcgtaga
     1201 gttcgcgacc gctcatgccg gtgctctccc atttcttgcg cacgatatgg aagagcttct
     1261 tggcgtccct ggcgcagcgc gcttggtcgg gcacgtcgcc gaccgtgaat gtatcgccgc
     1321 catcgccctc gaactcgccc cagacagggc cgatgtcgat aaggaagatg tcattgtcct
     1381 tcagcacaat gccagggtcg gacggggcgc cgaaggtctt tagcgtattg gttccgaagc
     1441 gcacgtagac atcgtgccag ccacgcacca tgtcgccttc ggcgagaaga tccttggcca
     1501 tggccacagc atcttcttcc accatgcctg ccttgcagcc agccgcgatg gcatggatcg
     1561 tggcacgggt cttggcccgc atttccagca tcttatcctt gagaaacgcg ggcccgacgg
     1621 cttccaattc gctttgcttt tcgaggactt cggtcatgct tggtctcctg ttaggatttc
     1681 gcgacgacgg gatgggtttg aaggcggagt cctgaaagcg actcaaccat agcaatctga
     1741 caggcaaggc gatgtgtccg cgtatcctcg ccacggccga gataaccgat cagccgaact
     1801 tcctgagcgg aggcaggcgg aagtgcggtg aacccttctt ccacccgcac ccggcaagca
     1861 ccgcagatgc cacggccctt gcagacggtc gccagcgcca cgcccgtccg ctgacaggcg
     1921 gcgagcacgc tttcgcccgg catagcggca acagccacgc catccagaaa aaactcaaac
     1981 aaaacagacc gttcttcggt gagcaggcag aggctgtcgt cgctgttggt catccagcat
     2041 ttttggaacg gaactgggcc ggtataaacg gccagtctca ttatttctgc tgggccagac
     2101 acgtccggag ctacaaactt gaacggaaac atttcgggcg tttaacccgc ggcaccactt
     2161 tcgtctgtca gcccgagccg cgtttttagc tctatgcgct tttgctgcag gatatcctga
     2221 gccttggccg gcgagccggt gatgcgcgct agcgcaatcg tgccggacat ttccgcaagc
     2281 agcgcggtcg cgtgcttcat cgcttcttgc tcgtcgcttc catccctg
//
LOCUS       DASWOG010000386         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_118928,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000386 DASWOG010000000
VERSION     DASWOG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118928"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VGB93_08010"
     CDS             complement(<1..509)
                     /locus_tag="VGB93_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0146083.1"
                     /translation="MTNLDRRAVLQSLAAVGAMTLAGRAWAAPQTNAKLLVVFLRGAY
                     DAANVIIPTGSEFYYSARPNLAIARPGSRPDQSKLEALALNADWSLHPAFRDSLLPLW
                     QKREIAFIPFVGTDDLTRSHFETQDTIELGQSIHAARDYRSGFLARLAGTLTGTKPIA
                     FTNQVPLIF"
     gene            complement(514..2022)
                     /locus_tag="VGB93_08015"
     CDS             complement(514..2022)
                     /locus_tag="VGB93_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0146084.1"
                     /translation="MRYRLWASGLLIGSFLFIGQGIAAPAYDAKTFLLLDRLTFGATA
                     GEAARLKVMGVNRWLDAELHPSAANNLPAQVQQQINALEMKPMNALVVDFSGKVKALK
                     AITDSDLRVAAAHAYQQELNDCAKQAAARSILRDLYSSSQLREVMTWFWFNHFNVHQY
                     KADIRAMVGDYEDTIRAHALGHFRDLVEATLRHPAMLRYLDNAQNAVGHINENYVREI
                     MELHTLGVGTGYTQQDVEALAHILTGVGIDVAPAPPHVKPELQDQIVRSGLFEFNPAR
                     HDYSDQIFLGHKIKGAGFAEVEEALDILARSPATARHISRELASYFVADDPPASLVEK
                     ISKTFLRSNGDIAAVLRTMIDAPEFSASSAKKLKDPVRYVMSAVRLAYEDKVILNTQP
                     VQNWLNRLAEGLYNYQTPDGYPLVASAWDGPGQIETRFEIARQIGSGSVGLFKPDMPG
                     AADNPAFPQLENALYFDGLQQTLRPATRAVLDEAQSPLDWNVLFLSSPDFMR"
     gene            2179..2355
                     /locus_tag="VGB93_08020"
     CDS             2179..2355
                     /locus_tag="VGB93_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146085.1"
                     /translation="MNDSDIQPPIHKGHDIRHAEIILRTHTLRNIVLASLAGVIFLGC
                     IWLIMVHASHHIVS"
     gene            complement(2375..2827)
                     /locus_tag="VGB93_08025"
     CDS             complement(2375..2827)
                     /locus_tag="VGB93_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0146086.1"
                     /translation="MSKPRVPFETTLLVRDSCLCLSVQQAARALARRFDDALRPLELT
                     NGQFSLLMSLNRPAPPKMGDVAQLLSMDRTTLTAALKLLERRGLVVTKSDDDDRRSRR
                     IALTRAGHALLLKALPIWKRHHAQLEKYLGGPNANKLRDDLEYLSRIA"
     gene            2914..3687
                     /locus_tag="VGB93_08030"
     CDS             2914..3687
                     /locus_tag="VGB93_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0146087.1"
                     /translation="MASKFVWYELMTSKAFAAETFYHDVVGWSARDAGMTNFKYTLLS
                     VGETPVAGLVQCPPDVPPGQSKPRWLGYIGVEDVDAKVASAEKLGAKVLHAPTDIPDV
                     GRFAVLSDPQGACFALFKPNGTGTPLRPMTPGTIGWHELHTKDAQKAFDFYSALFGWQ
                     QIQAMDMGPIGTYHIFGDENAPMGMGGIFNDAEAPQPHWLYYFMVADIDAAIARVTAA
                     GGKIIREPMQVPGGAWIIHARDPQTGLFALVGMRSANAA"
     gene            3761..3955
                     /locus_tag="VGB93_08035"
     CDS             3761..3955
                     /locus_tag="VGB93_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146088.1"
                     /translation="MATQIDYTLEPHLTVEEFRDVLVASTLAERRPASDLARLAPMLR
                     EADIIITARHEGRLVGVGAP"
     gene            4000..4170
                     /locus_tag="VGB93_08040"
     CDS             4000..4170
                     /locus_tag="VGB93_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0146089.1"
                     /translation="MAYQRRGIGKRLIEETRANAGPEATLVLLSAPSAESYYPKIGMK
                     KHGSCWTIARSR"
     gene            4454..>4793
                     /locus_tag="VGB93_08045"
     CDS             4454..>4793
                     /locus_tag="VGB93_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146090.1"
                     /translation="MGYLALSKDENCSLTSDTPGHLGGNTSSKYYRAAHQWIADSRQE
                     TPLCISGVQDGSGCLQGSAIIEYVPNDSAALGTQTVYIALIQEGADLRIASAFIMPDR
                     ETQRFSGFSEI"
BASE COUNT         1027 a         1348 c         1444 g          974 t
ORIGIN      
        1 tgaaagatga gcggcacttg attggtgaag gcgatgggct ttgtgcccgt cagcgttcca
       61 gcgaggcgcg cgaggaagcc agaccggtaa tcgcgcgctg catggatcga ctggccgagt
      121 tcgatcgtat cctgcgtctc gaaatggctg cgggtcagat cgtcagtgcc gacgaaagga
      181 atgaaggcga tttcgcgctt ctgccacagc ggcaggagcg agtcccgaaa cgccggatgc
      241 aggctccaat ccgcattgag cgcgagggct tcgagcttcg actgatccgg ccggctaccg
      301 ggccgcgcga tcgcgagatt gggccgcgcg ctgtaataaa attcgctgcc ggtcgggatg
      361 atgacattcg ccgcgtcgta agcgccacgc aggaaaacga cgagaagctt ggcgttcgtc
      421 tgtggcgcgg cccaggcccg gcccgcgagc gtcatcgcac cgacggcggc gagggattgc
      481 agcacagcgc ggcggtcgag attcgtcatg gaatcatctc ataaaatccg gcgacgaaag
      541 aaacaggaca ttccaatcca gcggcgattg cgcttcgtcc aaaacggcgc gggtcgcagg
      601 ccgcaacgtc tgttgcaaac catcgaaata aagcgcgttc tcaagctgtg ggaacgccgg
      661 attgtcggcc gccccgggca tatcgggctt gaaaagcccg actgagcccg acccgatttg
      721 ccgggcgatc tcaaaacgcg tctcgatctg gccggggcca tcccaggcgc ttgcgacgag
      781 cggatagccg tccggcgtct gataattata aaggccctcg gcaagacggt tgagccaatt
      841 ctgtaccggc tgcgtattga ggatcacttt gtcttcgtag gcgagccgca ccgcagacat
      901 cacatagcgg acgggatcct tcaatttctt cgccgaggag gccgaaaatt ccggcgcgtc
      961 gatcatcgtg cgcagaaccg cggcgatgtc gccattgctg cgcaggaatg tcttgctgat
     1021 cttctcgacg agcgatgccg gcggatcatc ggcgacgaaa tagctggcga gctcgcgcga
     1081 aatatgccgc gccgtggccg gactacgggc aaggatgtcg agggcttctt cgacctcagc
     1141 aaacccagcg cccttgatct tatggccgag gaatatctgg tcgctgtaat cgtgccgggc
     1201 cggattgaac tcgaaaaggc cgctgcgcac gatttgatcc tgcaattccg gcttcacatg
     1261 cggcggcgcg ggcgcaacat caattccgac gccggtcaag atatgcgcca gggcctcaac
     1321 atcttgctgc gtatagccgg tgccgacgcc gagcgtgtgc aattccatga tctcgcgcac
     1381 gtaattctca ttgatatgcc ccacggcatt ttgcgcattg tcgagatagc gcaacatggc
     1441 cggatggcgc agcgtcgcct caacaagatc gcggaaatgt ccgagggcgt gcgcgcggat
     1501 cgtatcttcg taatcaccga ccatcgcgcg gatgtcggcc ttgtattgat gcacattgaa
     1561 atgattgaac cagaaccagg tcatcacctc gcgcaattgg gaagatgaat agaggtcgcg
     1621 caaaatagac cgcgcggcgg cctgtttcgc acaatcgttt aattcctgct ggtacgcgtg
     1681 cgccgcagcg actcgcagat cggaatcggt aatggccttc agcgccttca ccttgccgga
     1741 gaagtcgacg acgagcgcgt tcatcggctt catctcgagc gcgttgattt gttgctgcac
     1801 ctgcgctgga agattgttgg ccgccgacgg atgaagttcc gcatcaagcc agcgattgac
     1861 tcccattacc ttgagccgtg ctgcctcgcc cgcggttgcg ccgaacgtca gccggtcaag
     1921 caacagaaaa gttttggcgt cgtaagcagg tgcagcaatg ccttggccga tgaagaggaa
     1981 cgacccgata agaagcccac ttgcccaaag ccgataccgc atcgcacgcg tcacctaatc
     2041 gaatggccta ttgaaattat cgccgtaatt gtgaaccttg tctgaaacag atcgggccat
     2101 tccgcggcag gagcttaacc gtgccgtggg aatcaaaccg gctaaggctc aaaagagcca
     2161 ataaagagag gaaacacaat gaacgacagt gatatccaac cgccgattca caaaggccat
     2221 gacatccgcc acgccgaaat cattctgcgc acgcatacgc tccggaatat cgtccttgcg
     2281 agcctcgccg gcgtaatttt tctcggctgc atttggctga tcatggtgca tgccagccat
     2341 cacatcgtaa gttgatctac gggcgcgggg gcttttaggc gatccgcgat agatattcga
     2401 ggtcgtcgcg caacttgttt gcgttagggc cgccaagata cttttcaagc tgggcgtgat
     2461 ggcgcttcca gatcggaaga gctttaagca aaagcgcatg cccggcgcgg gtcaatgcaa
     2521 tacggcggct tcgccgatcg tcgtcatccg actttgtcac gacgagcccg cgccgttcca
     2581 gaagcttcaa ggctgcggtt aaagttgtcc tgtccatcga cagcaattgc gcgacatcgc
     2641 ccatcttcgg cggcgcgggc ctgttgaggg acatcaacaa cgaaaattgt ccgttggtca
     2701 gctcaagcgg gcgcagcgca tcgtcaaatc ggcgtgccaa ggcgcgcgca gcctgttgca
     2761 cgctgaggca gaggcacgaa tcgcggacga gcagcgtggt ttcgaaggga actctcggct
     2821 ttgacataaa acgattatgt tgatatcaac ataatacgtc aagggctttg acgctcacgc
     2881 tttcgcgtgc gcggtgtgac cagggaggac gacatggcaa gcaagttcgt ttggtacgag
     2941 ttgatgacca gcaaggcttt cgcggccgaa accttctacc acgacgtcgt cggctggagc
     3001 gcacgcgacg cgggcatgac caatttcaaa tacacattgc tctcagttgg cgaaacgccg
     3061 gtcgcggggc ttgtgcaatg cccgccggac gtcccgccgg gccagagcaa gccgagatgg
     3121 ctcggctata ttggtgtcga agatgtggat gcaaaagtcg cctccgccga gaagcttggc
     3181 gcgaaagtgc tgcatgctcc gaccgatatt cccgacgtcg gccgctttgc cgtgctctcg
     3241 gacccgcaag gcgcgtgttt tgcgctattt aagccaaacg gcaccgggac gcctctccgg
     3301 ccgatgacgc ccggcacgat cggctggcat gagctgcata cgaaagatgc gcagaaggct
     3361 ttcgatttct acagcgcgct cttcggctgg cagcagatcc aggccatgga tatggggcca
     3421 atcggcacct atcatatctt cggcgacgag aacgcgccga tgggcatggg tggtatattc
     3481 aacgacgccg aggcgccgca gccgcattgg ctctattatt ttatggtcgc cgacattgac
     3541 gccgcgatcg cgcgtgtcac cgcagctggt gggaagatca tccgcgagcc gatgcaggtg
     3601 ccgggcggtg cctggatcat ccacgcgcgc gacccgcaga ccggattgtt tgcgcttgtt
     3661 ggtatgcgca gtgccaacgc cgcctagagc aaatccgatg cgagaaaacg gcgagaatcg
     3721 atggtaatct cacacggcgc ggaatttcgt ccgcgctgcg atggcaacgc agattgacta
     3781 cacgcttgaa ccgcacctga cggtggaaga attccgcgat gtgcttgtcg cctcgactct
     3841 ggcggagcgc cggccggcca gtgatcttgc acgtctcgcg ccgatgttgc gggaggcgga
     3901 cattatcatc acagcgaggc atgaaggccg gctcgtcggc gtgggggcgc cctaagcgat
     3961 ttcagctata gctgctatct ctcggatctc gcggtggacg tggcttatca acgccgcggg
     4021 attggcaagc ggctgatcga ggaaacccgc gcgaacgcgg ggccggaagc aacactggtc
     4081 ctgctctcgg cgccatcggc ggaaagctat tatccaaaaa tcgggatgaa gaagcatggg
     4141 agctgctgga cgattgcgcg gtcaagatag gagagcctta acaggaacgc cgctaggttg
     4201 agtcattgct cgttcaattg gatcgccgat gaaaatattt tcaggcgtgg ctctttcctg
     4261 gttttccgtg ctttcttcgt gctcgttgga tttgccgcac gataaaattg tcttcagcga
     4321 tttcagtcct agaacaggcg tcgaggtcgt ttgctcgtca gcgggacgtt tgactggctc
     4381 aacgatcacc gtggaatatc gaaaagaatc aggcagtttg ttcgctgtcg cttttgacgg
     4441 aaacgaaaac cagatgggct acctggcgtt gagcaaggat gaaaattgct cgctgacgtc
     4501 ggacacgccg ggacatctcg gcggcaacac ttcgtcgaaa tattaccgtg ctgcacatca
     4561 gtggattgcc gatagccggc aagaaacgcc gctctgtatc tcaggcgtgc aagatgggtc
     4621 aggctgtctt caaggctcgg cgatcatcga atacgtgcca aacgattccg cggcccttgg
     4681 cacgcagacg gtctatatcg ccctcattca ggaaggggca gatttacgga tcgcttcggc
     4741 tttcatcatg cctgatcgcg aaacccagcg gttcagcggc ttttccgaga ttc
//
LOCUS       DASWOG010000387         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_338655,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000387 DASWOG010000000
VERSION     DASWOG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338655"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VGB93_08050"
     CDS             complement(<1..1240)
                     /locus_tag="VGB93_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0146091.1"
                     /translation="MADQEPDRDRFSASKAWLRALELTARIDREPQRTLPKVIDELAA
                     RFGDAPALLSDHATLSFGELAGRVNAIARWTRAHGLGGQTVCLLMRNQPAYLAIWLGI
                     TRVGGVVALLNTNLRGDALTHCIKAAGADHIIVDASLLDTLDAALAQSRMSATVWVHG
                     GARPASFDDEINAFATDPLSLEEETGVSLSDRALCIYTSGTTGLPKAANVSHRRIMTW
                     SYWFAGILNASPADRTMNCLPMYHSVGGIVAIGSVLVSGGSVVLQEKFSARHFWDDIV
                     RWDCTLFQYIGELCRYLLKAPGNANERAHRLRLAFGNGLRADVWSEFQDRFNIPHIVE
                     FYAATEGTFSLINVEGEPGAIGRVPPFLKHRFLSALVKFDVARGIPHRDSDGFCAPCA
                     PDEIGEAIGKIGSRAEAGARF"
     gene            1453..2748
                     /locus_tag="VGB93_08055"
     CDS             1453..2748
                     /locus_tag="VGB93_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY0146092.1"
                     /translation="MRPETIAVHSGYTSEPTTKAVAVPIYQTVSYEFDSADHGAALFN
                     LEVEGFRYSRINNPTVDVLEKRVAALEGGVAALAVASGQAALNFALVNLADHGGNIVS
                     VPQLYGTSHTLLAHVLPRQGIQGRFAESDDAAAIERLIDADTRAVFCETIGNPAGNIC
                     DIEALAQAAHRHGVPLIVDNTVATPILWRPIEHGADIVVHSLTKFMGGHGTTLGGAIV
                     DSGNFPWAEHARRFPMFNEPDVSYHGLVYAERFGPRAFIERCRSVYQRTTGAVLSPHS
                     AFLLLQGIETVALRMERHVENARKVAEFLRADPRVDWVNYSGFADSPYYALAQKYLDG
                     QASSLLTFGVKGGFEAGKTFYDALGLIKRLVNIGDTKSLACHPASTTHRQMTAEEQRK
                     AGVRPEMIRISVGIEHATDIIEDLDQALAAVHDQSRAAE"
     gene            2745..3707
                     /locus_tag="VGB93_08060"
     CDS             2745..3707
                     /locus_tag="VGB93_08060"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF003776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEY0146093.1"
                     /translation="MSASADIAMQTKPSEERAADAQSLEIGIVNNMPDATLAATERQF
                     KALIDHATAGRRVRVRFFALPGITRGEGAARHIKETYSDLDELTRDKLDGLIITGCEP
                     KAPSLADEPYWPGLTRLIDWAADHTRSTIFSCLAAHAAVLHLDGISRVPVGRKYSGIF
                     ECAVVTDDPLTRGLGPTLHIPHSRLNDLDPAALSARDYRVLSASPKVGADIFVKRLGS
                     LFVFLQGHPEYDSESLLREYRRDVTRFLRGERPDFPAFPENYFDASLEGRLSSFAERV
                     ADGRSRQPHRELTSLLDGLHPARTWRASATGLYRNWMDGLSTRT"
     gene            complement(3714..>3820)
                     /locus_tag="VGB93_08065"
     CDS             complement(3714..>3820)
                     /locus_tag="VGB93_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0146094.1"
                     /translation="FGTTGTLVLGRMLGVILAALAAQFVIDGIRTITG"
BASE COUNT          745 a         1161 c         1187 g          727 t
ORIGIN      
        1 caaatcgcgc tccggcttcg gcgcgagagc caattttgcc gatcgcttcg ccaatttcgt
       61 cgggtgcgca gggtgcgcaa aagccgtctg aatcgcggtg gggtataccc cgtgcgacat
      121 cgaacttcac cagagcggac agaaaacggt gcttcaggaa gggcggaacg cggccgatcg
      181 cgccgggttc gccctcgaca ttgatcaggg agaacgtgcc ctcggtcgcc gcataaaatt
      241 cgacgatatg cgggatgttg aagcgatcct gaaactcact ccagacatcg gcgcgcaggc
      301 cgttgccgaa ggcaagccgc agacgatgcg cccgctcgtt ggcgttcccc ggcgctttaa
      361 gaagatagcg gcaaagttcg ccgatatatt gaaacagggt gcaatcccag cgtacgatgt
      421 cgtcccagaa gtgccgcgcc gaaaatttct cctgcagcac aaccgagccg ccgctgacga
      481 gcacgctacc gatggcgacg atgccgccga cgctgtgata catcggcaga caattcatcg
      541 tccgatcggc gggactcgcg ttcaaaattc cggcaaacca atagctccag gtcatgatcc
      601 gccggtggct gacattcgcc gcttttggca ggccggtcgt gccggacgtg tagatgcaga
      661 gcgcgcgatc cgagaggctg acgccggtct cctcttccag cgaaaggggg tctgtcgcga
      721 aggcgtttat ttcatcgtcg aaactggctg gccgcgcccc gccgtgaacc cagacggttg
      781 ccgacatccg cgactgcgcg agagccgcgt cgagcgtatc cagcaggcta gcgtccacga
      841 tgatgtgatc ggcgccggca gccttgatac aatgcgtcag ggcatcgccg cgcagattgg
      901 tgttgagcag tgcgaccacg ccgccaacgc gagtgatgcc gagccagatc gccaggtaag
      961 ctggctggtt gcgcataagg aggcaaaccg tctggccacc gagcccatgc gcgcgcgtcc
     1021 agcgggcgat ggcattgacc cgcccggcga gttcgccgaa gctcaaagtt gcgtgatcgg
     1081 agagcagggc aggtgcgtcg ccaaagcgcg ccgcaagttc atcgatcact ttcgggagcg
     1141 tgcgctgcgg ctcgcggtcg atccgcgccg tcagttcgag cgcccgcagc caggctttcg
     1201 aagcagaaaa gcgatcgcgg tccggctctt ggtcagccac tcgaaacatc cagcgaagag
     1261 gttaaaatac tttgcgatta tagccgacat gccatgagaa aggctaagct cttcttagcc
     1321 tccattggct atgaccggca cggtttctgc gccgaggcgt agcagggccc ggcgggagac
     1381 cattctgaga cgtgatgggt ctcgggctga gacaatagtt tcgaattgag tgtgtcgatg
     1441 cggaaatccg aattgcggcc agaaactatc gcagtccaca gcggctacac gagcgagccg
     1501 acgacgaagg ccgtcgcggt tccgatctat cagacggtgt cctacgagtt cgacagcgcc
     1561 gaccacggcg cggctctctt caaccttgag gtcgagggct tccgctacag ccggatcaat
     1621 aacccgaccg tcgatgtgct cgaaaagcgg gttgcggcgc ttgagggcgg cgtcgccgcg
     1681 cttgcggtgg cgagcggtca ggcggcgctt aatttcgcgc tcgtcaacct tgctgaccac
     1741 ggcggcaaca tcgtctccgt gccgcagctc tacggcacgt cgcacacgtt gctggcccat
     1801 gtcctgccac gccagggcat ccaggggaga tttgccgaaa gcgacgacgc ggccgcgatc
     1861 gagcgcctga tcgacgccga cacgcgcgcc gtcttctgcg agaccatcgg caacccggca
     1921 ggcaatatct gcgatatcga ggcattggcg caggccgctc atcgccatgg cgttccgctg
     1981 atcgtcgaca atacggtcgc aacgccgatc ctctggcggc cgattgagca tggcgccgat
     2041 atcgttgtcc attcgctcac gaaattcatg ggcggccacg gcacaacgct tggcggggcg
     2101 atcgtcgatt cagggaattt cccgtgggcc gagcacgcgc gccgctttcc catgttcaac
     2161 gagccggacg tttcctacca tgggctcgtc tatgcggagc gtttcggccc ccgcgctttc
     2221 atagagcgct gccgcagtgt ttatcagcgc acgaccggcg cggtgctctc gccgcatagc
     2281 gccttcctgc tgttgcaggg tatcgagacc gtcgccttgc gcatggagcg gcatgtcgag
     2341 aatgcccgca aggtcgcgga atttctccgt gccgatccgc gtgtcgactg ggtgaattac
     2401 agcggctttg cggatagccc ttattatgca ctggcgcaga aatatctcga cggccaagca
     2461 tcgtcgctgc tgacattcgg ggtcaagggc ggcttcgagg ctggcaagac attttacgac
     2521 gcgctggggc tcatcaagcg gctggtcaat atcggcgata cgaaatcgct cgcctgccat
     2581 ccggcatcga cgacacaccg gcagatgaca gcggaggagc agcgcaaggc cggcgtcagg
     2641 ccggagatga tccggatcag cgtcggtatc gaacatgcta ccgacattat cgaagatctg
     2701 gaccaggctt tggccgcagt acatgaccag agcagggcgg ctgaatgagc gcatcggccg
     2761 acatagcgat gcaaacaaag cccagcgaag agcgggccgc ggacgcgcaa tctctcgaaa
     2821 tcggcatcgt caacaatatg ccggacgcaa cccttgcggc gactgagcgg cagttcaagg
     2881 ccttgattga tcatgccacc gcggggcgcc gcgtacgggt gcggtttttc gcgctgcctg
     2941 gcattacgcg cggcgaaggc gcggcccggc atatcaaaga gacttatagc gatctcgatg
     3001 agctgacacg cgacaagctg gatgggctca tcatcactgg ctgcgaaccg aaggcgccgt
     3061 cgctcgccga cgagccttat tggccgggtc tcacgcggct catcgattgg gcagcggatc
     3121 acacgcgttc gacgattttc tcctgccttg ccgcgcacgc ggcggtactg catctcgatg
     3181 gaatctcccg cgtgccggtc ggcaggaaat attcaggcat tttcgaatgt gccgttgtga
     3241 cggacgatcc actgacgcgg ggtctcggtc ccacgttgca catcccgcat tcgcggctca
     3301 acgatcttga tcctgctgcg ctgtcggcgc gtgattatcg tgtccttagt gcatcaccga
     3361 aagtcggcgc cgatatattc gttaagcggc ttggcagcct cttcgttttt cttcaaggcc
     3421 atcccgagta tgattcggaa tcgctgctgc gtgaatatcg ccgcgatgtc acgcgcttcc
     3481 tacgcggcga gcggccggat ttcccggcat ttccggaaaa ttatttcgac gcctcgcttg
     3541 aaggccgtct gtcgtcgttt gcagaacgcg tggcggacgg gcgcagccgc cagccgcatc
     3601 gcgaactcac aagtttgctt gatggtctcc atccggccag gacttggcgc gcgtcggcca
     3661 ccgggctgta tcgcaattgg atggacggac tctcgacacg cacgtaggca cgttcagccg
     3721 gtaatcgtgc ggatgccgtc gatgacgaat tgtgctgcaa gggccgccag tatcacgccc
     3781 agcatccggc cgagcaccag tgtgcctgtg gtaccaaaaa
//
LOCUS       DASWOG010000388         7728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_81989,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000388 DASWOG010000000
VERSION     DASWOG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81989"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..429
                     /locus_tag="VGB93_08070"
     CDS             16..429
                     /locus_tag="VGB93_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY0146095.1"
                     /translation="MQTTDVRAVIAIGLRGQFGLHGRRPWEGNRSREFVADVERFFDL
                     TRRLVLIMGHRTFISVPEFAFRVRDIVEIHASDKPQEVLARFPGRAVFIGGGPVVYAA
                     YAPFIRHWDINRLPYDGEADRWFDPAWLVAGGDPV"
     gene            527..1831
                     /locus_tag="VGB93_08075"
     CDS             527..1831
                     /locus_tag="VGB93_08075"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY0146096.1"
                     /translation="MSSPLRLGVAGLGTVGAALLKLLDRQANALAERTGRRIAVTAVS
                     ARDRGKVRDLDLARFEWFSDPVALAASPEIDVFVELIGGADGVARAAVETALQDGKAV
                     VTANKALLAKHGLHLGKLAESKGAALGFEASVAGGIPIVKTLRDSLAGNSVSRVYGIL
                     NGTCNYILSRMETEQLSFAQCLAEAQKLGYAEADPSFDIGGYDTAHKLAILTALAFGT
                     EIAPDSIAVEGIEQITLADISAAAELGYRIKLLGVAARTAAGIEQRVHPTMVPRSSAI
                     AQVMGVTNAVTIDADAVQELTLVGPGAGGMATASSVVADIADIARGLRAPPFGLPMAS
                     LTPPRRAALQTHEGGYYIRLSVYDRPGAAAAIATRMAERTISLESIVQRRRPGEQKDA
                     SAVPVILITHATSEKLIGEALKDVLTDGFIAEPPQVIRIERE"
     gene            1840..2829
                     /gene="glpX"
                     /locus_tag="VGB93_08080"
     CDS             1840..2829
                     /gene="glpX"
                     /locus_tag="VGB93_08080"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0146097.1"
                     /translation="MTDLIVKGQDIIDRILTLELVRVTERAAIAAACLRGRGDEKGAD
                     QAAVDAMRRELHRLPIDGTVVIGEGERDEAPMLFIGEKLGTGGSAVDIAVDPLEGTTL
                     CAHDMPGSIAVMAMATGGALLHAPDVYMEKIAVGPGYPRGVVDLDKSIEENVAALAKA
                     KGVSSKEISVLLLDRPRHKPILEACREIGCPVRLIPDGDVAGVIFTTRPKETGIDLYV
                     GIGGAPEGVLAAGALRCVGGQMQGRLILDTDEKIARAAQMGVKDPKRKYDLTELAAGD
                     VIVSLTGVTDGALVSGVKFDEDAIRTETLIYRSTTGTVRRIATEHRQKARARA"
     gene            complement(2834..3277)
                     /locus_tag="VGB93_08085"
     CDS             complement(2834..3277)
                     /locus_tag="VGB93_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146098.1"
                     /translation="MRFLGLVIAPAFALLSISTQAAGLLDRSANRLPDVVEQDNGGNL
                     LNRIEFVEHHRNDNVYRLPLECFSSCTLLLGISGACVTQGSVLYFHGATIDGKLAPAY
                     LNNYAASYYPANVKRWVAAHHALQKVSFTRMTWQQAAAIGVKVCP"
     gene            complement(3380..3691)
                     /locus_tag="VGB93_08090"
     CDS             complement(3380..3691)
                     /locus_tag="VGB93_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0146099.1"
                     /translation="MTMGHIIWIIVIGFVAGMLARWFSSAPNTPSGFIFTVLLGIAGA
                     FLATFIGQSVGWYRLDQGAGLIGATVGAVVLLLIWRLFSGKFGGAGNAGPAATGPRRW
                     L"
     gene            complement(3787..5019)
                     /gene="glp"
                     /locus_tag="VGB93_08095"
     CDS             complement(3787..5019)
                     /gene="glp"
                     /locus_tag="VGB93_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY0146100.1"
                     /translation="MDAQKLLPVAEARARVLAGVGPRGTERVPLAAALGRTLSEPLLA
                     RRTQPPFRASAMDGFALKAVDLESLPATLKLIGESAAGHGFTDALNRGETVRIFTGAP
                     VPNGADTVLIQEDATLDGDTVTATRTVVEGRHIREIGIDFADGEVLLPVGTRLGPIDL
                     ALAAASGHAEVAVARKPRVAILATGDELVQPGEAASVDQIVASNSFAVAAFVQQAGGE
                     AIDLGIAGDDFAAIEAAVLAARQAEADVLVTLGGASVGKHDLVRSALTNEGMELGFWR
                     IAMRPGKPLIHGRLGDMPILGLPGNPASSIVCALLFLIPLLRALNGDPRAADDPSEPA
                     ILGAPVAPNGPREDYLRAKLNRSTDGLPIATPHDVQDSSLLRVFAHSQCLLVRPPDTP
                     AAAAGDVCRVIKLSPVLL"
     gene            complement(5025..5504)
                     /gene="moaC"
                     /locus_tag="VGB93_08100"
     CDS             complement(5025..5504)
                     /gene="moaC"
                     /locus_tag="VGB93_08100"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0146101.1"
                     /translation="MAKLTHLAEAGQAHMVDVSEKPTTDRTARAEGHVAMRKETLDLA
                     LAGNSKKGDVFAAARIAGIMAAKKTADLIPLCHPLALTKVTVDIEPDAALPGLRVAAE
                     AKVSSQTGVEMEALTAVSVACLTIYDMLKAADRTMHIEGIALVEKSGGRSGLFRRGD"
     gene            complement(5504..6334)
                     /gene="trpC"
                     /locus_tag="VGB93_08105"
     CDS             complement(5504..6334)
                     /gene="trpC"
                     /locus_tag="VGB93_08105"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY0146102.1"
                     /translation="MADILKQIEAYKRKEIAEAKLRMPLSTLERRIENHDPPRGFAHA
                     IETKLAEGRFALIAEIKKASPSKGLIRDDFDPPALAKAYEKGGAACLSVLTDTPSFQG
                     KLDYLEAARKVVRLPALRKDFLFDPYQVYEARAYGADCILIIMASVTDEEAKKINKTA
                     HDLRLDVLVEVHDEAELHRALELETRLIGINNRDLRTFETKLETCERLAAKVPADRIV
                     VAESGIATHEDCLRLHKSGIDTFLVGESLMRQHDVAAAAHLLLCGDESERHAARKAHG
                     "
     gene            complement(6412..7440)
                     /gene="trpD"
                     /locus_tag="VGB93_08110"
     CDS             complement(6412..7440)
                     /gene="trpD"
                     /locus_tag="VGB93_08110"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY0146103.1"
                     /translation="MDAFKPLLAKIATGASLSRADAEHAFNLLLGGTVTAAQTGAFLM
                     ALRVRGETVEEITGAVAAMRAKMLPVAAPQNAVDIVGTGGDGHGTYNVSTLTALIVAA
                     CGVPVAKHGNRAASSKSGSSDVLIALGVKIGLSPDEVAYCLDEAGIGFMTAPVHHAAM
                     RNVGQIRTELGTGTLFNLLGPLSNPARVRRQLLGVFAKEWLEPLAEVLRNLGSERVWL
                     VHGADGLDEVTTTGPTYVTALENGVIRSFVITPEEAGLQPANLADLKGGDAAHNAAAL
                     NAVLHGARNPYRDIAVLNAAATLIVAEKAETLREGVATAAAALDAGRAAEVLARLIKA
                     SHGGAKGI"
     gene            complement(7480..>7728)
                     /locus_tag="VGB93_08115"
     CDS             complement(7480..>7728)
                     /locus_tag="VGB93_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146104.1"
                     /translation="SPIGLSLVTKLAPTRMLSLVMGCWLATSFTGNFLAGWLGSFWSR
                     MGRVEFFLTTAAIAAFAGLVIAVCSRALVNITSERGAV"
BASE COUNT         1420 a         2383 c         2473 g         1452 t
ORIGIN      
        1 agcgggagag ttttcatgca aaccactgac gtgcgcgccg tcatcgccat cggccttcgc
       61 ggccagttcg gccttcatgg ccggcgaccg tgggaaggca atcgcagccg cgaatttgtt
      121 gccgatgtcg agcggttctt cgatctgacg cgccgccttg tcctgatcat gggccaccgc
      181 accttcatct cggtgcccga attcgccttc agagttcgcg acatcgttga aatccacgcc
      241 tccgacaagc cgcaggaggt gttggcgcgg tttcccggcc gggccgtgtt catcggcggc
      301 ggccctgtgg tctatgccgc ctatgcgccc ttcatccgcc attgggatat caaccgcttg
      361 ccgtacgatg gcgaggccga tcgctggttc gatcccgcct ggctcgtggc tggcggcgat
      421 ccggtctagc tccggccttt tgttgatcgc cccgacgaaa tggggcacaa cccgcccaac
      481 atgacgggcc ggttctcaag ccggcgattc agcagagatt tttgcgatgt cttctccctt
      541 gcgtctcggc gtggccggtc tcggcaccgt tggcgctgcc ctcttgaagc ttctcgaccg
      601 tcaggccaat gctttggcag agcgaaccgg acgtcggatc gcggtgacgg ccgtctccgc
      661 ccgggatcgc ggcaaggtcc gcgatctcga tctggcgcgt ttcgaatggt tcagcgatcc
      721 ggtcgctttg gccgcctctc ccgagattga cgttttcgtc gaattgatcg gcggcgcaga
      781 cggcgtggcg cgcgccgcgg tcgagaccgc gttgcaggac ggcaaagccg tcgttaccgc
      841 caataaggca ctgctggcaa agcacgggct gcatctcggc aaactggccg aatcgaaggg
      901 cgccgctctc ggtttcgagg cttcggtcgc cggtggcatt ccgatcgtca agacgttgcg
      961 cgacagcctt gcgggcaatt cggtgtcgcg cgtttatggc atcctgaacg gcacctgcaa
     1021 ttacatcctc tcccggatgg agacggagca gctttctttc gcgcaatgtc tcgccgaggc
     1081 gcaaaagctt ggctatgccg aggcggatcc ttctttcgac atcggtggtt acgacacggc
     1141 gcataagctg gcgattctga cggcgctcgc cttcggcact gagatcgcgc ccgattccat
     1201 cgccgtagaa ggcatcgagc agatcacgct cgccgatatt tcggcagccg ccgagcttgg
     1261 ctaccggatc aagctgcttg gcgtcgctgc ccgcaccgca gcggggattg aacagcgcgt
     1321 gcatccgacg atggtaccgc gttcgtccgc catcgcgcag gtcatgggtg tcaccaatgc
     1381 ggtgacgatc gatgccgatg ccgtgcagga attgacactg gttgggccgg gggccggcgg
     1441 catggcgacg gcgtcgtcgg tcgttgccga cattgccgac attgcccgcg gcctgcgcgc
     1501 gccgcctttc ggcttgccga tggcgtcgct gacgccgccg cggcgcgccg cgctgcagac
     1561 gcacgaaggc ggctattaca tccggctttc ggtttacgac cggcccggcg ccgcagcagc
     1621 gattgcgacc cgcatggccg agcgcacgat ttcgctggag agcatcgtcc agcgccgccg
     1681 tccgggcgag cagaaggacg cgtcggctgt gcccgtgatc ctcatcaccc acgcgacgtc
     1741 ggagaagttg atcggtgaag cgttgaaaga cgtgctcacc gacggcttca tcgccgagcc
     1801 gccgcaggtc atccgtatcg agcgcgaata ggagttttcg tgactgatct tatcgtcaag
     1861 ggccaggaca tcatcgatcg catcctgact cttgaacttg tccgcgtcac tgaacgcgct
     1921 gccattgccg ctgcatgcct gcgggggcgc ggcgacgaaa aaggcgccga tcaggccgcc
     1981 gtcgatgcga tgcggcgcga attgcatcga ttgccgatcg acggcaccgt cgtgattggc
     2041 gaaggcgaac gcgacgaggc gccgatgctc ttcatcggcg agaagctcgg aaccggcggg
     2101 tcagcggtcg atatcgcggt tgatccactc gaaggcacaa ccctctgcgc acacgatatg
     2161 cccggctcga ttgcggtgat ggcgatggcg accggcggcg cgcttcttca tgcccccgat
     2221 gtttacatgg aaaaaatcgc cgtcggaccg ggctatccgc ggggcgtggt cgatctcgac
     2281 aaaagtatcg aagaaaatgt tgctgcgctc gccaaagcga agggtgtgtc gtcgaaagag
     2341 atttctgtcc tgttgcttga ccggccgcgg cacaagccga tcctcgaagc ctgccgcgaa
     2401 atcggctgcc cggtgcggct catccccgat ggtgatgtcg cgggcgtgat cttcacgacg
     2461 cgtccgaagg agaccggtat cgacctttac gtcggcatcg gcggcgcgcc ggaaggcgtg
     2521 ctcgcggcgg gcgccttgcg ctgtgtcggt ggccagatgc agggccgtct cattcttgat
     2581 accgacgaaa aaatcgcgcg cgcggcccag atgggtgtca aagatccgaa gcgcaaatat
     2641 gatctcaccg aattggccgc aggggatgtc atcgtgtcgc taaccggcgt cactgatggc
     2701 gcgctcgtgt cgggcgtcaa attcgacgag gatgcgatcc gcaccgaaac tctcatctat
     2761 cgttcgacga cagggacggt gcgccggatc gccaccgagc atcgtcaaaa ggcccgcgcg
     2821 cgcgcttaaa gcgctacgga cagaccttca cgccgatagc agcggcttgc tgccaggtca
     2881 tccgcgtgaa gctgactttt tgcagggcgt ggtgcgcggc gacccagcgt ttcacgttgg
     2941 ccggataata gctcgcggcg taattgttga gataggcggg cgcgagcttc ccgtcgattg
     3001 tcgcgccgtg aaaatacagt accgagccct gggtaacgca ggcgcctgaa atgccgagta
     3061 gcagcgtaca agacgagaaa cattcgagcg gcaggcgata aacattatca ttgcggtggt
     3121 gctcgacgaa ttcgatgcgg ttcaacagat tgccgccgtt gtcctgttcg acgacatcgg
     3181 gcaggcgatt ggctgaccta tcgagaaggc cggcggcttg ggttgaaatg gatagcagcg
     3241 caaacgccgg tgcgatcacc aaacccaaaa accgcatcgt gctgtcccct gctatccgaa
     3301 cctgcatttc agcagattcg gcaagcaatg tccgcgcctc ggcgacggtt agagagacga
     3361 tccaccagct ttagacgact tagagccagc ggcgggggcc cgtcgcagcc ggtccagcat
     3421 tgccggcgcc gccgaacttg ccagagaaga ggcgccagat cagcagaagg acaacagcgc
     3481 cgacagtcgc gccgatcaaa ccagcgcctt gatcgagccg ataccagcct acggactggc
     3541 cgatgaaagt cgccaaaaat gcccccgcga tgccaagcaa aaccgtgaaa atgaacccgc
     3601 ttggcgtgtt cggggccgac gaaaaccacc gcgccagcat gccggcgacg aagccgatga
     3661 cgataatcca gatgatatgg cccatggtca ttctccccag ttctggtcgc ggattcggtc
     3721 aggccagctt ttgaaacgag cttaagcggg ctttcaggtc tgtcaatctg accggccgga
     3781 caggttttag aggagaaccg gcgacagctt gatgactcgg cacacatcgc ccgctgctgc
     3841 cgccggtgta tcgggcggtc gcaccagcag acattgggaa tgtgcgaaaa cgcgcagaag
     3901 tgacgaatcc tgcacatcgt gaggcgtcgc gatgggcaga ccgtcggtgc tacggttcag
     3961 cttggcgcgc aggtaatctt cgcgcggacc gtttggcgcc accggcgcgc cgagaattgc
     4021 gggttcggac ggatcatccg ctgcgcgcgg atcgccgttc agcgcgcgca ataagggaat
     4081 gagaaagagc agggcgcaga cgatcgacga ggccggattg cccggcaatc cgaggatcgg
     4141 catatcgcct aaacgaccgt gaatcagcgg tttgccaggc cgcatggcga tgcgccagaa
     4201 acccagctcc ataccctcat tggtgagggc tgagcggacg aggtcatgct tgccgaccga
     4261 agctccgcca agcgtgacaa gaacatcggc ttcagcctgg cgtgcggcga ggacggcggc
     4321 ttcgatcgca gcgaaatcgt cgccggcgat accaaggtcg attgcctcgc cccccgcctg
     4381 ttggacgaaa gcggcaacgg cgaaggaatt cgacgcgacg atctgatcga cgctcgccgc
     4441 ctctccgggt tgcacgagtt cgtcgccggt cgccagaatt gcgacgcggg gcttccgcgc
     4501 gacagcgacc tcggcgtgac cgctggctgc cgcgagcgca aggtcgatcg ggcctagccg
     4561 cgtgcccaca ggaagcagca cctcgccatc ggcgaagtcg atgccgatct cacggatatg
     4621 ccggccttcg acgacggttc gggtcgccgt gaccgtatct ccgtcgagag tcgcgtcttc
     4681 ctgtatcagc accgtatcgg cgccgttagg caccggcgcg ccggtgaaga tgcggaccgt
     4741 ctcgccgcgg ttcaacgcgt cggtaaagcc gtggcccgcg gcgctttcgc cgatcaattt
     4801 taacgtggcg ggcagcgatt ccaaatctac ggccttcagg gcaaacccat ccatcgccga
     4861 agcgcgaaat ggcggctgcg tgcgccgcgc gagtaggggc tcactcaaag tgcgcccgag
     4921 tgctgcggca agcggcacgc gctcggtccc gcgcgggcca acgcctgcga ggacgcgcgc
     4981 ccgcgcctcg gccacgggaa gaagtttctg tgcatccatg tcgttcaatc gccgcgccgg
     5041 aaaaggcctg atcggccgcc tgatttttcg acaagcgcga taccttcgat gtgcatcgtg
     5101 cggtctgcag ccttcagcat gtcgtagatc gtgagacagg cgacggacac ggccgtcagc
     5161 gcctccattt cgaccccggt ctggctgctt accttcgctt cggcggcgac gcgcaggccg
     5221 ggcagggcgg cgtcgggctc gatatcgacc gtcaccttag tcaacgcgag cggatggcaa
     5281 agcgggataa ggtcggcggt tttcttcgcc gccatgatgc cggcgatccg cgctgcagcg
     5341 aagacgtcgc cctttttgga attgcctgcg agagcgagat cgagggtctc tttgcgcatc
     5401 gctacatggc cttcggcgcg ggcggtgcgg tcagtcgtcg gcttttcgga cacatcgacc
     5461 atatgcgcct ggccggcctc ggccagatgg gtgagcttcg ccatcagccg tgcgctttgc
     5521 gcgcggcgtg tcgctcgctc tcgtcgccac acaacagaag atgcgccgcg gccgcgacgt
     5581 cgtgttggcg catcaggctt tcgccgacga ggaacgtgtc gatgccggat ttgtgcagac
     5641 gcagacaatc ttcatgcgtc gcgattccgc tttccgcgac gacgattcgg tcggcgggga
     5701 cttttgccgc gaggcgttcg cacgtctcga gtttcgtctc aaacgtccgg agatcgcgat
     5761 tattgatgcc gatcagccgt gtctcgagtt cgagcgcgcg atgaagctcg gcctcgtcat
     5821 ggacttcgac gagcacgtcg agccgcagat cgtgcgcggt tttgttgatc ttcttggcct
     5881 cctcgtcggt gacactcgcc atgatgatga gaatgcaatc ggcgccataa gcgcgtgcct
     5941 cgtaaacttg gtaaggatcg aagagaaaat ccttgcgcag tgctggcagg cggacgacct
     6001 ttcgcgcggc ttcgagataa tcgagcttgc cttgaaatga gggggtgtcg gtcaggaccg
     6061 aaaggcaagc cgcgccgcct ttttcgtaag ccttggcgag cgccggcggg tcgaaatcat
     6121 cgcggatgag gccctttgaa gggctggcct ttttgatctc cgcgatcaag gcaaagcggc
     6181 cctcagcgag tttcgtttcg atcgcgtggg cgaagccgcg cggcggatcg tgattttcga
     6241 tccggcgttc cagcgtcgac agcggcatgc gcaatttggc ttccgcaatc tctttgcgct
     6301 tataggcttc gatctgcttc agaatatcgg ccatgggtac cctctacagc ccccgcaaaa
     6361 tcactctgcc acgaagcgga gcgaaggcaa aatggcagac gttgctggat atcaaatccc
     6421 tttagcaccg ccatgcgacg ctttgatcag ccgtgcgagc acttcggcgg cacgacccgc
     6481 atcgagagcg gcggcggcgg tggcgacacc ttcgcgcagc gtctcggcct tttccgcgac
     6541 gatcagtgtc gccgcggcgt tcaggaccgc gatatcgcga tagggattgc gcgcgccgtg
     6601 caggacggca ttgagggccg ccgcattatg cgcggcatca ccccctttga gatcggccag
     6661 gtttgccggt tggagcccgg cctcctctgg cgtgatgacg aaggagcgga tcacgccatt
     6721 ttcgagcgcc gtgacatagg tcgggccggt ggtcgtcacc tcgtcgagcc cgtccgcgcc
     6781 atggaccagc cagacgcgct ctgaaccgag attgcgcagc acctccgcca gaggctccaa
     6841 ccattctttt gcaaaaaccc ccagcaattg ccgccggacc cgcgccggat tggaaagcgg
     6901 accgagcagg ttgaacaggg tgccggttcc gagctccgtg cggatctgtc cgacattgcg
     6961 catggcggca tgatggacgg gtgctgtcat gaagccgatg ccggcctcgt cgagacaata
     7021 ggcaacttcg tccggcgaca ggccgatctt gacgccaagc gctatcaaaa cgtcgctcga
     7081 tcccgatttc gacgaggcgg cgcgattgcc atgtttggcg acgggcacgc cgcaggcggc
     7141 gacgatcaat gcggttaacg tcgagacatt ataggtgccg tggccatctc cgcccgtgcc
     7201 gacgatatcg accgcattct gcggtgccgc cacgggcagc attttggccc gcatggccgc
     7261 gaccgcgccg gtaatttcct cgaccgtctc accgcgcacc cgcaacgcca tcagaaaggc
     7321 gccggtctgt gccgcggtca ccgtgccgcc aagcaggaga ttgaaggcgt gttctgcgtc
     7381 ggcgcggctg aggctcgcgc cggtggcgat cttcgcgagg aggggcttga aggcgtccat
     7441 ggtgtctcat tgctctggca gacgcgtaag cgcaagatct tagacggcgc ctcgctcgct
     7501 cgtgatgttg acgagtgcgc ggctgcagac ggcgatgaca agtccggcga aggctgcgat
     7561 ggcggctgta gtcagaaaaa attcaacccg gcccatgcgg ctccagaagc ttccgagcca
     7621 gccagcgaga aaattgccgg tgaagctcgt cgccaaccaa cagcccatga cgagtgaaag
     7681 catccgtgtg ggcgccaact tcgtcaccag ggacagaccg attggcga
//
LOCUS       DASWOG010000389         5953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_356948,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000389 DASWOG010000000
VERSION     DASWOG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356948"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /gene="hypF"
                     /locus_tag="VGB93_08120"
     CDS             <1..1790
                     /gene="hypF"
                     /locus_tag="VGB93_08120"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEY0146105.1"
                     /translation="AAFGLCEDCAADYGNPTSRRFHAETIACPVCGPRLSHPIEEIAT
                     ALRRGQIIALKSVGGFHLMCDAANEAAVEALRRRKLRPVKPFAVMVANGASVERIAAP
                     SVPEMALVREIARPIVLMRARAGLASSVAPGLDRVGIMLPSAPVHHLLFHALAGPDGS
                     AWRDERQPLALVATSANAAGQPLIIDDTEALRGLARIADMIVTHDRPIFVRADDTVMA
                     VIDGAPAYVRRSRGFVPEPIDLGQDGPPVLAVGAHLKATLCVTRGREAFVSQHVGDLN
                     TADTIRFYEETARHMLEMLDVVPEIVACDLHPDYRSTLFAEATGLPILRVQHHAAHLS
                     AVAAEHHLRGRVLGVALDGHGYGDDGGAWGGELMLQEGAGWRRLGHLLPLALPGGDRA
                     AREPWRMGVGALTALGRGTEAAEHFPDIAFAGPLAAFVTADGQGPATTSMGRLFDAAA
                     ALLGVCTQQSYEGQAAIELEALVRIPRCLPAGYRIAGNILDFRPLLARLLEPGLQARE
                     GAELFHGTLIAGLAAWISFGASQTGQTDVVLSGGCFMNRILTEGLAAALRVRGLVPWL
                     PRAAPANDGGLSLGQAAMARAHLMAESMP"
     gene            1807..2055
                     /locus_tag="VGB93_08125"
     CDS             1807..2055
                     /locus_tag="VGB93_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEY0146106.1"
                     /translation="MCLAIPIRVEELLPDDMARVTLSGVSKIVSIALLEDVRLGDYLL
                     IHVGYALARLDPDEAERTLAMMHEAAVPGLPDMETSRL"
     gene            2052..3197
                     /gene="hypD"
                     /locus_tag="VGB93_08130"
     CDS             2052..3197
                     /gene="hypD"
                     /locus_tag="VGB93_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEY0146107.1"
                     /translation="MKHIDEYRDGNLARSMAATITREAVAGRHYQFMEFCGGHTHAIS
                     RYGIEDLMPANVQMVHGPGCPVCVLPIGRIDEAIRLTMRPEVTLCTYADLMRVPASRG
                     ANLLKAKAAGADIRMVYSTLDAIRIAETEPDREVVFFAIGFETTTPPTAVAIRLARKK
                     RLSNFSVFCNHVLTPSAMRGILDSPDARAVRVDGFVGPAHVSTITGTTPYVSIAAEFS
                     KPVVVAGFEPLDVVQAILMLVRQVNEGRHEVENQYIRLVTAGGNLIAQREMADIFELR
                     PSFEWRGLGELPMSALRLRDAYADLDAERRFAIETVPAADNPACECGDVLRGLKKPAE
                     CKLFGTLCSPETPMGSCMVSPEGACAAHWIYGRFRDSARAVAEKAVA"
     gene            3194..4243
                     /gene="hypE"
                     /locus_tag="VGB93_08135"
     CDS             3194..4243
                     /gene="hypE"
                     /locus_tag="VGB93_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEY0146108.1"
                     /translation="MSAPRLRTRKLDIQNGRVDLSHGAGGRAMAQLIDEIFRESFDNP
                     FLDRGNDQAAFDVPAGRMVMSTDGYVISPLFFPGGNIGSLAVHGTINDIAMGGARPLH
                     LAASYIIEEGFPLADLQRIAISMGHAALEAGVAIVTGDTKVVERGEGDGVFISTTGIG
                     IVPQGLDLSAELARPGDRVILSGCIGDHGVAVMSKRSNLGFETEILSDSAALHELVAE
                     MVRVAGSSLRVMRDPTRGGLAATLNEIAQQSGNGFLIEEGRILVRPEVAAACELLGLD
                     PLYVANEGKLVAIVAPDAADVLIAVMRAHALGRDAAVIGEVTEDVQCFVQMTTSFGGG
                     RIVDWLAGEQLPRIC"
     gene            4822..>5953
                     /locus_tag="VGB93_08140"
     CDS             4822..>5953
                     /locus_tag="VGB93_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0146109.1"
                     /translation="MTYQSFNPATGKLLKKFEELTDQQLENKIAAAETCFEIWRHKSY
                     AERAAIVAKAAGLLHAKAAEFAHTMTLEMGKRISEALGEVEFSSNILSYYAKNAERFL
                     APVTLHPSLGEGHMESSPIGVVFCVEPWNFPYYQLARIAGPHLMAGNVVLVKHAGIVP
                     QCAIAFEKLWIDAGAPVGLYTNLLISHDQSDRVVDDERIKGVALTGSVAAGRSIAARA
                     GKNLKPSSMELGGSDAFIVLEDADLDHTIKWAVWGRMYNTGQTCCAAKRFVVVEKLAD
                     EFLEKFKAALTALKPGDPLDAKTTLGPLSSESALVDLLKQVDGAVSHGAKVLIGGKRI
                     DRPGSYMEPTILTDIKPGNPAFREEFFGPVALFFRVKDENEAI"
BASE COUNT         1097 a         1807 c         1863 g         1186 t
ORIGIN      
        1 tggcggcttt cggcctctgt gaggattgtg ctgccgacta cggcaacccc accagccgcc
       61 gctttcacgc tgagacaatc gcttgtccag tgtgtggtcc gcgtctgagt catccgatcg
      121 aggagatcgc gaccgccctg cgtcgcggac agatcatcgc gctgaaaagc gtcggtggat
      181 tccatcttat gtgtgatgcg gcgaacgagg cggcggttga agcgctgcgc cgccgcaaac
      241 ttcgtcccgt gaagcctttc gcagtcatgg tcgcgaacgg ggcatcggtc gagcgtatcg
      301 ccgcaccgag cgtacccgaa atggctctgg tccgcgagat cgctcgccca atcgtgctaa
      361 tgcgggcccg tgccggattg gcctcctcgg ttgccccggg attggaccgt gtcgggataa
      421 tgttgccctc tgcgcccgta catcatctct tgttccatgc tttggccggg cctgatggtt
      481 cggcgtggcg cgatgagcgc cagccgcttg cgctcgtcgc taccagcgcg aacgcggccg
      541 ggcagccatt gatcatcgac gataccgagg cactacgtgg cctggcgcgc atcgccgaca
      601 tgatcgtaac tcacgatcgc ccgatctttg tccgtgcaga cgataccgtc atggccgtga
      661 tcgatggtgc gcccgcctat gtccggcgct cgcgcggctt cgtccccgaa ccgattgatc
      721 taggtcagga tggcccgccg gtactcgccg tcggcgcgca tctcaaggcc acgctctgcg
      781 tcactcgcgg acgcgaggcg ttcgtgtcgc agcatgtcgg cgatctcaac acggcggaca
      841 caatccgttt ctacgaggaa accgcgcgac acatgctgga gatgctggat gtcgtgccgg
      901 aaatcgtcgc ttgcgatctg catccggact atcgctccac cctgtttgcc gaggcgacgg
      961 gattgccgat cctgcgcgtc cagcaccacg ccgctcatct ttctgccgtc gccgccgaac
     1021 atcatctgcg aggacgggtc ttgggagtgg cactcgatgg gcatgggtat ggcgatgacg
     1081 ggggtgcttg gggcggtgag ctgatgctgc aagaaggcgc cggttggcgc aggctcggcc
     1141 atctcctgcc gctcgcgctg cctgggggag accgcgccgc gcgcgagccc tggcgcatgg
     1201 gcgtcggcgc tttgacggcg cttgggcgcg gcacggaagc agccgaacat tttccagata
     1261 tcgcgttcgc cggccccctg gcggcgttcg ttaccgcaga cgggcaaggg ccggcaacca
     1321 caagtatggg tcgattgttc gatgccgccg ccgcgctgct cggagtatgt actcagcaaa
     1381 gctacgaggg ccaggcggcc atagagctcg aggcccttgt ccgtatcccc aggtgcctgc
     1441 ctgccggcta tcgcatcgcc ggcaacattc tcgatttcag gccgcttctt gccaggcttc
     1501 tggaaccagg tctgcaggcg cgcgagggcg cggagctgtt tcatggcacg ctgatcgccg
     1561 gcctcgctgc atggatcagc ttcggcgcct cgcaaacggg tcagaccgac gtcgtcctca
     1621 gcggcggctg cttcatgaac cgtatcctga cggaggggct tgccgccgca ctgcgggtac
     1681 gcggcctcgt gccatggctt ccgcgtgccg cgcccgcaaa tgacggcggc ctctcactcg
     1741 gacaggcagc tatggcacgg gcgcatctta tggccgaaag catgccctaa cgctcgaggt
     1801 agtccaatgt gcctagccat cccgatccgc gtcgaagagc ttcttcccga cgatatggcg
     1861 cgcgtcacgc tgagcggtgt ctcgaagatc gtgtccatcg cgcttctcga ggacgtccgg
     1921 cttggcgact atctgctcat ccatgtcggt tatgcgcttg ccaggctgga cccggacgag
     1981 gcggagcgaa cccttgccat gatgcatgag gccgccgttc caggcctccc cgatatggag
     2041 acatctcggt tatgaaacat atcgacgagt accgcgacgg taacctcgcg cgttccatgg
     2101 ccgccaccat cactcgtgag gctgtcgccg gccgacacta tcagttcatg gagttctgtg
     2161 gcggccatac ccacgccatt tcacgctatg ggatagaaga cctcatgcct gcaaacgtgc
     2221 agatggtgca tgggccgggc tgtcccgtct gcgtcctgcc gatcggacgc atcgacgagg
     2281 caatccggct caccatgcgg cccgaggtca cgctatgcac ctatgccgat ctcatgcgcg
     2341 tgcccgcctc ccgaggggcc aatctgctca aagccaaggc cgctggcgcc gatatccgca
     2401 tggtctattc tacactggac gcgatccgca tcgccgagac ggaacctgac cgtgaggtcg
     2461 tattcttcgc gatcggcttc gagacgacca cgccgcccac tgcagtcgct atccggctgg
     2521 cgcgcaagaa gcggttgtcg aatttcagcg tgttctgcaa ccacgttctc actccctctg
     2581 cgatgcgtgg aatcctcgac tcgccggatg cccgcgccgt gcgggtcgat ggcttcgtcg
     2641 gccccgcgca tgtcagcacg atcacaggca ccacgccgta tgtgagcatc gctgcggaat
     2701 tctcgaagcc ggttgtcgtt gccggcttcg aaccgctcga tgtggtgcag gcgatcctaa
     2761 tgctggttcg ccaggtcaac gaggggcggc acgaggtgga gaatcaatac atccgtttgg
     2821 tgaccgcggg cggcaacctg attgcgcagc gcgagatggc cgatattttc gagctgcgtc
     2881 catcgttcga atggcgtggg ctgggcgaac tgcccatgag cgcactgcgc ctacgcgacg
     2941 cctacgccga tctggatgcc gaacggcgct ttgcgatcga aacggtcccg gcggccgaca
     3001 atccggcctg cgaatgcggt gacgtattac gcggcctcaa gaagccggcc gaatgcaaac
     3061 tgttcggaac gctctgttcc cccgagacgc cgatggggtc ctgcatggta tcacccgagg
     3121 gggcctgtgc ggcgcactgg atctatggcc gcttcagaga cagcgctagg gccgtcgcgg
     3181 aaaaggccgt cgcttgagcg cgcccaggct ccgtacgcgc aagctggata tccagaacgg
     3241 tcgagtcgac ctttcccacg gagctggcgg ccgggccatg gctcaactca tcgatgagat
     3301 ctttcgtgag tctttcgaca atccctttct cgatcgcggc aacgatcagg ctgcgttcga
     3361 tgtgccagcc ggacggatgg tgatgtcgac cgatggctac gtgatttcgc cgctgttctt
     3421 tcccggcggc aacatcgggt ccctggccgt ccatgggacg atcaatgaca tcgcgatggg
     3481 cggcgctcgt cctttgcatc tcgccgccag ctacatcatc gaggaaggct ttccgcttgc
     3541 cgatttgcag cggattgcca tcagcatggg gcacgccgcg ctcgaggcgg gcgtggcgat
     3601 cgtaacgggc gacaccaagg tcgtcgaacg cggcgagggc gacggcgttt tcatctcgac
     3661 gaccggcatc ggcatcgtgc cccagggcct cgatctctcg gctgaacttg cccggccggg
     3721 agaccgggtc attctttcag gctgcattgg cgatcatggc gtggcggtaa tgtcgaagag
     3781 gagtaatctc ggattcgaga ccgagatcct gtccgacagt gccgcgttgc acgaactcgt
     3841 tgcagagatg gtgcgcgtgg caggttcgtc gctgcgggtt atgcgtgacc cgacgcgcgg
     3901 tggcctagcg gccaccctca acgaaatcgc gcagcaatcc gggaatgggt tcctgatcga
     3961 ggagggcagg atcctcgtcc gccccgaagt agccgccgct tgcgaactcc tggggctgga
     4021 cccgctctac gtcgccaacg aaggcaagct cgtcgccatc gtggctccgg atgcagcaga
     4081 tgttcttatt gcggtcatgc gtgcccatgc gcttggccgc gatgcggctg tcatcggaga
     4141 ggtcacggaa gacgtgcaat gtttcgtgca aatgacgacc tcgttcggag gcggtcggat
     4201 cgtagattgg ctcgcgggcg agcaactccc caggatctgc tgaagacacg gactacctaa
     4261 attttgccga actccggcca ataacaatca accaagcctg gacaagaagc gagggtaacg
     4321 gggacaaggg caacggggcc aatcttgcat ctgaagctag agcataggac cgaaaagtgt
     4381 gacgcggttt tcggactaat ccgatgcgat taaaaagaca taagaataac gaggcgattc
     4441 catatgaacg tccttcgctc tggacgcaga tgaagctgag ccagttcgtc ggccaagccg
     4501 gatttcttgg cgacgggtct gagtagtttc cgagtctacc gctcctgccg tgccgaggcc
     4561 tacgttccga tccgtcgagc agccagttta aaggctgaaa gatcgcagct tgcatgatcg
     4621 cgcggcaaac cgaaactcgc gtccgattca caacagcaga gatgtcgccg atggaatcgc
     4681 tcgctcgtcc aacgtgatta tcgaggtttt tggcttgatt cgggatctgg atacccgttg
     4741 cgagcatcga tcaagcttct agtgacactt gcgatggatc ggtccgcgtt tcgtatgacg
     4801 atattgcata aggacaaatc gatgacctac caaagtttca atcccgcgac cggcaagctc
     4861 ctgaagaaat tcgaggagct tacggaccaa caactcgaga acaagattgc cgcggccgag
     4921 acctgtttcg aaatttggcg gcacaagagc tatgccgaac gcgcggccat cgtggccaag
     4981 gcggcgggtc tgctgcatgc caaggccgcg gagtttgcgc atacgatgac gctggagatg
     5041 ggcaagcgca tcagcgaggc tctcggagaa gtcgagttca gctcgaacat tctctcctat
     5101 tacgccaaga atgccgaacg cttcttggcg ccggtgacgc tccatccatc cctcggcgaa
     5161 ggccatatgg aaagcagccc gattggcgtg gtcttctgcg tggagccgtg gaactttccc
     5221 tattatcagc ttgcccggat cgccggtccg catttgatgg cgggcaacgt cgtgctcgtg
     5281 aagcacgcag gcatcgtgcc gcaatgtgcc atcgcgttcg agaagctgtg gatcgacgcg
     5341 ggcgcgcccg tcgggctgta taccaattta ctgatctcgc acgatcagtc ggatcgcgtt
     5401 gtcgatgacg agcgtatcaa aggggtcgcg ctgaccggca gcgttgcggc tggcaggagc
     5461 atcgcggctc gagccggaaa gaatttgaag ccatcgtcga tggaactcgg tggcagcgac
     5521 gcgtttatcg tcctcgagga cgccgatctc gaccacacca tcaagtgggc tgtctggggt
     5581 cgaatgtaca atacgggtca gacctgttgc gccgcgaaga gattcgttgt cgtcgaaaaa
     5641 ctcgctgacg agttcctgga gaagttcaag gcggcgctca cggctctgaa gccaggcgac
     5701 ccgctggatg caaagaccac actcggtccc ttgtcctcgg aatcggcctt ggttgacctt
     5761 ttgaaacagg ttgatggtgc ggtctcccac ggggcgaagg tgctcatcgg cggcaagcgc
     5821 atcgatcgtc cggggtcgta tatggagccg acgatcctga ccgacatcaa gccgggcaac
     5881 ccggctttca gagaagagtt tttcggccca gtcgcgctct tcttccgggt caaggacgaa
     5941 aacgaggcga tcg
//
LOCUS       DASWOG010000390         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_302886,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000390 DASWOG010000000
VERSION     DASWOG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302886"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VGB93_08145"
     CDS             complement(<1..1411)
                     /locus_tag="VGB93_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146110.1"
                     /translation="MRFNRALISAFFVWAFSFAAHAQPAPQSDSDKAPTILACFNAGQ
                     TAIRDLRACAQAWLTPTALLLCALQNTDAGKAGPIACPFLPDTPNGRAILDALLEQQY
                     LTRDSNLSLDTRNFPGLPSAATVDACNKTAATQQDFLACIQTSMRHDQSQELLQCVGA
                     TDSESGRALCLAKSVAADPDLPATVKCFGAQGAAPDKFASCLGAPDRVAAAKAAIGCA
                     AQAGTSASALADCLLPNASPAEKALAACLVQSEDDDRSVAGCLGNFSPAMGRAEQASA
                     CFSDPGNSAAKCAGVLLGGTTHVSEIVAACAGVRRNAQVGCFLDKRPELAATQRLYGC
                     FANGGEGSSLVAACTGELVPDEKIRRTLACATVANNDPATLAVCAAGSVLSDDAAHLA
                     ECAATSQDSPVSFALCAAAPKTDDNWRISAQCAVSSAGDSNSYEGCMGTPLAMNELGR
                     CFKGKFGKDCFGPGNTLAKI"
     gene            complement(1470..>1927)
                     /locus_tag="VGB93_08150"
     CDS             complement(1470..>1927)
                     /locus_tag="VGB93_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0146111.1"
                     /translation="YGLHQGIADILGSDHAPHTLEEKAKPYPQSPSGMTGVQTLVPIM
                     LDHVNAGRLSLERFVDMTSAGPARLFGIAGKGRVAVGYDADFTLVDLKRRETIADQWS
                     ASRSGWTPYDGVTVTGWPIGTIVRGQRVMWQGEIVTPGQGDAVRFIGSL"
BASE COUNT          367 a          607 c          593 g          360 t
ORIGIN      
        1 gtattttggc gagggtgttg cccggcccga aacaatcctt gccgaatttg cctttgaagc
       61 acctgccaag ttcgttcatc gcgagcggcg ttcccataca gccttcatag gagtttgaat
      121 cgcctgccga tgagacggcg cattgcgcgg aaatccgcca attgtcatcc gttttcggcg
      181 cagccgcaca taacgcgaaa gaaacaggcg agtcttgcga ggtggcggcg cattcggcga
      241 gatgcgccgc gtcatccgac aaaaccgaac ccgctgcaca gacggcaagt gttgcaggat
      301 cattgttcgc gacggtggcg caggcaagcg tccggcggat tttctcatcc gggacgagct
      361 cgccggtgca agccgcgacg agcgaactgc cttcgccgcc gttcgcgaaa cagccgtaga
      421 gcctttgcgt cgccgccagc tccggtcttt tatcgaggaa acagccgacc tgcgcgttgc
      481 gccgcacgcc ggcacaggcg gcaacgattt ccgatacgtg agttgtgccg cctaaaagca
      541 cccccgcgca tttcgcggcg ctattccccg gatcggaaaa gcaggccgag gcttgctcgg
      601 cacgccccat cgcaggcgaa aaattgccga gacatccggc gacgcttctg tcgtcatctt
      661 cagattgcac aaggcaggct gcgagcgctt tttcggcagg gctggcgttt ggcaaaaggc
      721 aatcggcgag cgccgatgcg gaggttccag cctgcgcggc gcatccgatc gccgccttcg
      781 ccgccgcgac ccgatcaggg gcgccgaggc aggatgcaaa tttgtcgggc gcggcgccct
      841 gcgcgccaaa acatttgacg gtggcaggca gatccggatc ggctgccacg cttttcgcaa
      901 gacacaaggc gcgcccgctc tccgaatcgg tcgctccgac acattgtaaa agctcctggc
      961 tttgatcgtg gcgcatcgac gtctgaatac aggcgaggaa atcttgctgt gtcgcggctg
     1021 tcttattgca agcgtccaca gtggcggcgc tggggagacc ggggaagttg cgcgtatcca
     1081 gggacagatt gctgtcgcgc gtcaaatatt gctgctccag aagagcatcc aggatcgcgc
     1141 gtccattagg cgtatccggc agaaagggac acgcaatcgg accagctttc cctgcgtccg
     1201 tgttttggag cgcgcacagc agcagggccg tgggcgtcag ccacgcttgg gcgcaggcac
     1261 ggagatcgcg aatcgccgtc tgcccggcat tgaaacaggc gaggatcgtc ggcgctttgt
     1321 cactatccga ttgcggcgcc ggctgagcgt gcgcggcgaa ggaaaaagcc caaacgaaaa
     1381 aagccgagat caacgcccga ttgaatcgca tatgcacgtc ctcccacgcg atgccccggc
     1441 gttgttatgc ttttgcgggc gtttgcactt tatagactgc cgataaaccg caccgcgtca
     1501 ccctgccccg gcgtgacgat ctcgccctgc cacatcacgc gctgcccgcg cacgatggtg
     1561 ccaattggcc agcccgtgac cgtgacaccg tcataaggcg tccacccgct gcgtgacgcg
     1621 ctccattgat cggcgatcgt ctcgcggcgc ttgagatcga cgagcgtgaa atcggcatcg
     1681 tagccgacgg ctacccgtcc cttgccggcg atgccgaaga gccgtgccgg cccggcgctt
     1741 gtcatgtcga cgaagcgttc aaggctcagc cgcccggcat tcacatgatc gagcatgatc
     1801 ggcaccagcg tctgcacgcc ggtcatgccc gatgggcttt gcggataggg cttcgccttc
     1861 tcttccagcg tgtgcggcgc gtggtccgag ccgagaatgt cggcaatgcc ttgatgcaac
     1921 ccatacc
//
LOCUS       DASWOG010000391         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_137503,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000391 DASWOG010000000
VERSION     DASWOG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137503"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGB93_08155"
     CDS             complement(<1..1005)
                     /locus_tag="VGB93_08155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0146112.1"
                     /translation="MQIAMVGSGYVGLVSGACFADFGHKVCCIDNDPAKIEALKYGKM
                     PIYEPGLAELVMTNVRQNRLTFADDLSAVREAQAVFIAVGTPSRRGDGHADLTYVYQA
                     ARDIAAAINGFTVIVTKSTVPVGTGDEVERIIREVRPDAEFAVVSNPEFLREGAAIAD
                     FKRPDRIVIGVEDPRAEAVMREVYRPLYLNQAPILVTSRRTSELTKYAANAFLATKIT
                     FINEIASLCEEVGANVQEVARGIGLDNRIGSKFLHAGPGYGGSCFPKDTQALIKTAQD
                     YGAPVRIVETVAAVNDQRKRAMARKVLAACGGNVRNKTIAVLGLTFKPNTDDMRDSPA
                     I"
     gene            1235..>1787
                     /locus_tag="VGB93_08160"
     CDS             1235..>1787
                     /locus_tag="VGB93_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146113.1"
                     /translation="MTNALQYDLCVLGGGGAGLTAARTAARLGAKVVVIEKRALGGAY
                     LTQTIPLQAFCTAALHTGAALGAEIDFVHVRTQALAAVKDFARDYAPEPLNASGIHLI
                     RALGSFSSPARVEAGGQLIEAKHFILATGATPAHASWPGQELIRPLMLEDVLTIDRPP
                     EDLIIIGANFQALILAQAFLRLGT"
BASE COUNT          333 a          565 c          552 g          337 t
ORIGIN      
        1 gatcgccggc gaatcgcgca tgtcgtcagt attgggcttg aaggtgaggc cgagcacagc
       61 gatcgtcttg ttgcgtacgt tgccgccgca cgcggccagc actttgcgcg ccatggcgcg
      121 cttgcgctgg tcgttcacgg ctgcgaccgt ctcgacgatg cggaccggcg cgccataatc
      181 ctgcgccgtt ttgatcagcg cctgcgtgtc cttggggaag caggagccgc cgtagccggg
      241 cccggcatgc agaaattttg agccgatgcg attgtcgagg ccgatgccgc gcgcgacctc
      301 ctgcacattc gcgcccactt cctcgcagag gctcgcaatc tcgttgatga acgtaatctt
      361 ggtcgcaagg aaggcgttcg ccgcatattt ggtcagttcc gacgtgcggc ggctggtgac
      421 gaggatcggc gcctggttga gatagagcgg ccgatagacc tcacgcatca cggcttcggc
      481 acgcgggtcc tcgacgccga tgacgatgcg atccggccgc ttgaagtcgg cgatcgcagc
      541 gccttcgcgc aggaactccg gattggagac gacggcaaat tcggcgtccg gccgcacttc
      601 gcgaatgatg cgttcgacct cgtcgccggt gcccaccggg acagtggatt tcgtcacgat
      661 gaccgtgaag ccattgatcg ccgcggcaat gtcgcgcgcc gcctgataga cataggtgag
      721 atcggcatgg ccgtcgccgc gtcgcgacgg cgtgccgacg gcgatgaaca ccgcctgcgc
      781 ctcacgcaca gccgagagat cgtcagcaaa tgtcagacgg ttctgacgga cgttggtcat
      841 caccagttcg gccaggccgg gctcatagat cggcatcttg ccgtacttca gggcctcgat
      901 cttcgcggga tcattgtcga tgcaacagac cttgtgaccg aaatccgcga aacaggctcc
      961 cgagaccagg cccacatatc ctgagccgac catagcgatt tgcattcttc gctccttaag
     1021 cgccgcacgc ccggcaaggc ttgcggccga aacctagcca gtgccatttt cgtgaccgag
     1081 aggcaaccga cgcacgaatt ggcgggccga ggccaaaatc gcacggtctg ttcgccgacc
     1141 atactttcgg caagtcattg tcgagactaa agaacttaag gtgcggctga agacggggca
     1201 agcccgccac caccgcagtg gcgcgagata gattatgaca aatgcattgc aatacgatct
     1261 ctgcgtgctc ggcggcggcg gtgcggggct gacggcggcg cggacggcgg cgcggctcgg
     1321 cgcgaaggtt gttgtcatag aaaagcgcgc cctaggcggc gcctatctca cccagacgat
     1381 tccgctccag gctttctgca cagcggcttt gcacactggc gcggcactgg gcgcagaaat
     1441 cgattttgtc cacgttcgca cccaggccct cgctgcggtc aaggatttcg cgcgcgatta
     1501 tgcgccggag ccactcaatg cctccggcat ccatttgatc cgcgctctcg gcagtttcag
     1561 tagccccgca cgcgtcgaag ccggcggtca attgatcgag gcgaaacatt tcattctcgc
     1621 caccggcgca acaccggcac acgcctcatg gcccgggcag gaactcattc ggccgctgat
     1681 gctcgaggac gtgctcacga tcgaccggcc acccgaagat ctgatcatca tcggcgcgaa
     1741 ttttcaggca ctcatcctgg cgcaagcgtt tctgcggctt ggcacgc
//
LOCUS       DASWOG010000392         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_27493,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000392 DASWOG010000000
VERSION     DASWOG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27493"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1166
                     /locus_tag="VGB93_08165"
     CDS             333..1166
                     /locus_tag="VGB93_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146114.1"
                     /translation="MGRLEIIGMADGADTPEPSAHWDIAGQAIAAGKFGGFPRIAPSR
                     SLIVWPINPAYDISALTTAEGKLADPDIEWDDVEGDAVLNPPSADDEADTTQPALSPN
                     QYYAGLIEDRVDAPLPKIDVGTVTWNGAAQISTQKTHGPVEVAEIDSVPADISARIFV
                     CCRAPAGATPSQLILDVVFASQPKTDKIDGLEDPEARQTGDIHGELLHAEIQTQGANW
                     FRFLLSTTPGDFDENFKLLISRPWIDIPVRFESGRRAILTFATGKAGADAIYAAVHST
                     D"
     gene            1230..2555
                     /locus_tag="VGB93_08170"
     CDS             1230..2555
                     /locus_tag="VGB93_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY0146115.1"
                     /translation="MLQNKTLDANFYQVGDGSRLARLSNFTIFVIGALFLFLLIWSAF
                     AEIDEVTRGDGKVIPSQKIQSIQSSEPGVVEQILVRLGQHVAKGDLLIRLDNTMTESN
                     LGEDEAKARSLEAQIARLEIERTGDRTALYVCPDDLKAVAADVCATEALLLQTRRAEQ
                     SQKLDSLREKIEERQREFNEAKDESQRIAQGLGLAQQEFDLISPMVQKRIAPKTDLLN
                     VERDLVDLKGQQAANQQVIARTDAALREANNNLADAALQIQSDALADLSKASAEFSVI
                     RETIKGAKEHNRRTDIRSPVDGIINTLDTNTIGAFVNAGSHVMDIVPLEDKLLIETKV
                     KPRDIAFVRPGQKALVKLTAYDFSIYGGLKGVVQQVSADSVYDDKAHETFYIILVATD
                     ESTLRHNGATYPIMPGMISQVDIVTGRKTILQYLLKPIFKARDESLTER"
     gene            2644..3240
                     /locus_tag="VGB93_08175"
     CDS             2644..3240
                     /locus_tag="VGB93_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY0146116.1"
                     /translation="MKFRILKRQGWKASFRLEEIFWQVLGDAARSKNIKIADYVKDAI
                     DRAGSDSRNQSSALRVHAAEFLVARCRALQEHGEARSSINAALAAPSPCFVLSSARKL
                     INHNREFYDFVSAAGALVSPMGVSSALLTLDAEVPRLIDLLASKPGNALTCGYALRID
                     ARLLRGKARVVLVAQNDAKLLVGYILDSAASVRPPPAA"
     gene            complement(3218..>3942)
                     /locus_tag="VGB93_08180"
     CDS             complement(3218..>3942)
                     /locus_tag="VGB93_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146117.1"
                     /translation="AVEHGKLEFRNVAFQYPLNTRLVLDGINLTISPGEKVGIIGKVG
                     SGKTTFGRLVAGFYAPAKGEILVDGVDLRQYHPHELRKAVGLVMQDTDLCIGSIKANL
                     LLANPSASDADLIKACKLAGVDDFVSLHPLGYDLPVGERGRNLSTGQKQAVALARVLL
                     MNPMILFLDEPSSAMDLATEQNFLMRLKQMMRPDQTLLITTHRHSMLELVDRIIILDN
                     GRVVADGSKANVLNALKRPAAV"
BASE COUNT          876 a         1172 c         1113 g          781 t
ORIGIN      
        1 atgtttttat gcgtgccgcg gcgctttttt tccttattgg cgttgttgca agcatcctga
       61 gcccggtgcc cgcatttgcg gcagagatcc agccgacctc tcacaaccag aagtcgaaga
      121 ttggtcacgt aatatctgaa aaaacattgc gcaaaaatag gcgaattgcg cagatacaga
      181 agacgagacg ggcggcgtcg gccggagcgt cgcacaagcg atttgcaggg tctcgagcgg
      241 agatcaaacg gcctcggtca acgcatcctg cgacgcccga cacgacgaag cttgcgcggc
      301 cggaaaccgt tgacgcgcga tccgccgcct ggatggggcg cctcgaaatc attggcatgg
      361 ccgacggcgc cgatacgccg gagccgtcgg ctcattggga catcgccggg caagcaattg
      421 ccgccggcaa atttggcggc tttccgcgga ttgcgccgtc gcgatcgttg atcgtctggc
      481 cgatcaatcc cgcttatgac atttccgcgc tcacgacggc ggaaggcaag ctcgccgacc
      541 cggacattga gtgggacgat gtcgaaggcg atgcggtact aaatccaccg tctgccgacg
      601 atgaagccga cactacgcag ccggccctat cgcccaatca atattacgcc ggcctgatcg
      661 aggatcgggt ggatgcgcca ctgccaaaga tcgacgtcgg caccgtcacc tggaacggcg
      721 ctgcgcagat atcgacgcag aagacgcacg gccccgtgga agttgcagag atcgacagcg
      781 tgccagccga catctcggcg cggatctttg tctgctgccg cgcccccgcc ggggcgacgc
      841 catcgcagct cattctggat gtcgtcttcg cgtcccagcc taaaaccgac aagatcgatg
      901 gactcgaaga cccggaggcg cgccagacgg gcgatatcca tggtgaactg cttcatgcgg
      961 agatccagac gcaaggcgca aactggttcc gtttcctcct gtccacaacg cccggcgact
     1021 tcgacgagaa tttcaaactg ctgatctcgc gaccatggat cgatatcccg gtacgatttg
     1081 aatccggacg gcgggcgata ttgacattcg cgaccggcaa agccggcgcg gatgcaatct
     1141 acgctgccgt tcactctaca gattgattgt catcgttgcg cgcgcgggtt cgcgctatat
     1201 ctgattgccc ttgagaggac gccgtgtttt tgctccaaaa taaaacgctc gatgcgaatt
     1261 tctaccaggt cggtgacgga agccgactcg cacgactttc gaatttcacg atcttcgtga
     1321 tcggcgccct attcctgttt ttgctcattt ggtctgcctt cgccgaaatc gacgaagtta
     1381 cccgcggcga cggcaaggtt atcccttcgc agaagattca atcgatccag agttcggagc
     1441 cgggcgttgt cgaacaaatc ctcgttcgcc tcggccagca tgttgcaaaa ggcgatttgc
     1501 tgatccgcct cgacaatacg atgaccgagt ccaacctcgg cgaagacgag gcgaaggcac
     1561 gcagcctcga agcccagatc gcgcggctcg aaatcgaacg caccggcgat cggacagcac
     1621 tctatgtctg tcccgatgac cttaaggctg tcgccgccga tgtctgcgca acggaagccc
     1681 tccttctcca gacccggcgc gcggaacaaa gccaaaagct cgactccctt cgcgaaaaga
     1741 tcgaggagcg ccaacgcgaa ttcaacgagg cgaaagacga atcacaacgg atcgcccaag
     1801 gcctgggcct ggcgcagcag gaattcgatc tgatcagccc gatggtgcaa aagcggatcg
     1861 cgccgaaaac cgacctcttg aacgtcgaac gcgatctcgt cgacctgaag gggcagcaag
     1921 ccgcaaacca gcaggttatc gcgcgcaccg atgcggcgct tcgcgaggcg aacaataatc
     1981 ttgccgatgc cgcgctgcag attcagtccg acgcgctggc cgacctcagc aaggcgagcg
     2041 ccgagttttc agtcatcaga gagaccatta agggcgccaa agagcacaat cggcgcaccg
     2101 acatccgttc gccggtcgat gggatcatca atacactcga caccaatacg atcggtgcgt
     2161 tcgtcaacgc gggcagccat gtgatggaca tagttccgct cgaggacaag cttctcatcg
     2221 agacaaaggt gaagccgcgc gatatcgcct ttgtacggcc agggcagaaa gccctcgtaa
     2281 agctcaccgc ctatgacttc tcgatctatg gcggtcttaa aggcgtcgtc cagcaggtct
     2341 cggccgacag cgtttatgac gacaaggcgc acgagacttt ttacatcatc ctcgtcgcga
     2401 ccgacgagag cacattgcgg cacaacggcg cgacgtatcc gatcatgccg gggatgatct
     2461 cacaggtcga tatcgtcacg ggcaggaaga caatcctgca atatttgctg aaacccattt
     2521 tcaaggcgcg cgacgaatcg ctgaccgagc gctgaatttt actcgtcctc caggcgatcg
     2581 cttttcgaga tggaaccaaa agccgagaca actcccgcca acgaacctgc ttcagcttcg
     2641 tctttgaagt ttcgtatcct caagcgtcaa ggctggaagg ccagtttccg cctcgaggaa
     2701 atcttctggc aagtcttagg tgatgcggcg cgatcgaaga acatcaagat cgcggattac
     2761 gtcaaggacg cgatcgaccg cgccgggagc gattcgcgca atcaaagctc cgccctgcgt
     2821 gtacatgccg ccgaatttct tgtggcacgt tgccgtgcgc tgcaggaaca tggcgaagca
     2881 agaagttcga ttaacgctgc gcttgccgcg ccctcacctt gctttgtgct cagctctgct
     2941 cgtaaactca tcaatcacaa tcgcgaattc tatgacttcg tttcggcggc gggcgcgctc
     3001 gtctcgccta tgggggtttc atcggccctt ctgaccctcg atgcagaagt tccgaggctg
     3061 atcgatctcc tggcaagcaa gccggggaac gcgctgactt gtggctacgc gctacggatc
     3121 gatgcgcgtc ttcttagagg caaagcccgt gtcgtgctcg tggcgcaaaa cgacgccaag
     3181 ctgcttgtcg gctatatttt ggattcagcg gccagcgtca gaccgccgcc ggccgcttga
     3241 gcgcgttgag aacgttcgcc ttgctgccat ccgcaacgac gcgcccgtta tcgagaatga
     3301 tgatccgatc gacgagttcg agcatactgt ggcgatgcgt cgtgatgagc aacgtctgat
     3361 ccggccgcat catctgcttg agacgcatca gaaagttttg ttccgtcgca aggtccatcg
     3421 cactcgacgg ttcatcgaga aacaaaatca tcggattcat cagcaagacc cgggcgagcg
     3481 caacggcctg cttttgtccc gtcgaaaggt tgcggccgcg ctcgccgacc ggcaaatcat
     3541 aaccgagcgg atggagcgag acaaaatcgt cgacgccggc gagcttgcaa gccttgatga
     3601 ggtcggcatc cgaggcgctt ggattggcga gcagcaaatt ggctttaatc gacccgatgc
     3661 aaagatcggt atcctgcatg accaaaccga cagctttgcg gagttcatgc ggatgatatt
     3721 ggcgcagatc gacgccatcg acgaggattt cgcctttagc cggcgcgtag aatcccgcca
     3781 ccaatcgtcc gaaggtcgtc ttgccggaac cgaccttacc gatgatgcca actttctcac
     3841 caggcgatat cgtgagattg attccgtcga gcacaagccg cgtattcaac ggatattgaa
     3901 atgcgacatt gcggaattcg agcttgccat gttcgacggc cc
//
LOCUS       DASWOG010000393         3245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_27510,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000393 DASWOG010000000
VERSION     DASWOG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27510"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGB93_08185"
     CDS             <1..327
                     /locus_tag="VGB93_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY0146118.1"
                     /translation="RGLAMAQEITIARVYLREADKGNRESLMREILKILHDERRVKGV
                     TVFRGIAGFGETGEVTSGDPLHILSSLPVVIEFFDSPENVEPAIQALTSLLPADHIVS
                     WPAKQH"
     gene            398..664
                     /locus_tag="VGB93_08190"
     CDS             398..664
                     /locus_tag="VGB93_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0146119.1"
                     /translation="MISASQVRAARGLLGWSQERLSEQAHIPLAGLLDYETGAREMPQ
                     MSAANLKRALERAGVIFSEDESGVSLLPPPEVVPLEKLNSSNDE"
     gene            complement(665..1078)
                     /locus_tag="VGB93_08195"
     CDS             complement(665..1078)
                     /locus_tag="VGB93_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY0146120.1"
                     /translation="MSLNGLSSNGLITVPSNYSVRETLDRLEAALKAAGITIFARVDH
                     AGGAAAVGLALRPTEVIIFGSPKGGTPLMQANQMAGLDLPLRVLAWEDEAGNCSLTYN
                     DINWLAARYDLGAMTEPAREGLTKVLAKFTAKAAS"
     gene            complement(1088..2002)
                     /locus_tag="VGB93_08200"
     CDS             complement(1088..2002)
                     /locus_tag="VGB93_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146121.1"
                     /translation="MSKLPDFEAWAVFAKVAELGSFAQAASELGLSKATVSKVVGRLE
                     ARLGTTLFHRTSRRMSLSEAGRTTLERASRILAEGEAVEAEATAQSAVPRGLVRVAAP
                     MTFGIVHLAPLLPDFFKLCPDVSVDLSLSDQIVDIVSEGFDLALRISSLPDSSLLARR
                     LCQVRVLLVGAPAYFGQHGRPEHPKDLAAHIALLYSYSRSRDVWHFSHPKQGEYSVAV
                     SGPIRANNADVLQPSLCAGAGLALQPEFLVWRDIAEGRLEAVLKDWSAPPAALHLVTP
                     PSPMRPVRVQVLMDFLAQRFTRMPWAQV"
     gene            2112..2810
                     /locus_tag="VGB93_08205"
     CDS             2112..2810
                     /locus_tag="VGB93_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0146122.1"
                     /translation="MIERRPFDSLGGAKHGWLDAKHHFSFGDYGDAKRMGWGALRVWN
                     DDTIAPQTGFPPHPHRDMEIITYVREGAITHQDNLGNKGRTEAGDVQAMSAGTGIRHA
                     EYNLEPATTRIFQIWIMPREQGLGPAWGAKPFPKDDRAGQFVTLASGFDDDKDALPIR
                     ADARVVGATLKAGQTANYPIGETRHAYLVPAKGSVKVNDVTLDARDGAAVYGEKVLHV
                     TALEDAEVVLVDAA"
     gene            2875..>3245
                     /gene="wrbA"
                     /locus_tag="VGB93_08210"
     CDS             2875..>3245
                     /gene="wrbA"
                     /locus_tag="VGB93_08210"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY0146123.1"
                     /translation="MAKVLVLYYSAYGHIEKMAEAVAEGARETGAKVDIKRVPETVPV
                     EIAQASHFKLDQAAPVATIADLADYDAIILGTGTRFGRLSSQMASFLDQAGGLWARGA
                     LNCKVGAAFSSTATQHGGQETT"
BASE COUNT          687 a          980 c          971 g          607 t
ORIGIN      
        1 agaggtttgg ccatggcaca agaaatcacc attgcgcgtg tctatctccg ggaggccgat
       61 aaaggcaatc gcgaatcttt gatgcgggaa attctgaaaa ttttgcatga tgaacggcgc
      121 gtcaagggtg taacggtctt ccgtggaatc gccggtttcg gtgagaccgg cgaggttaca
      181 agcggcgatc cgcttcatat tttgtcgagt ctcccggtag ttatcgaatt tttcgacagc
      241 ccggagaatg tggagcccgc tattcaggcg ctgaccagcc tgttacccgc cgaccacatc
      301 gtttcctggc cagcaaagca acattgaatg caacctttct aaatttggcg cgtttcggtt
      361 ggcgggttcg tgtaattcgg gggactaaaa tttggacatg atctcagcgt ctcaagtacg
      421 tgccgcgcgc ggcctccttg gctggtcgca ggaacgcctt tcagaacaag cgcatattcc
      481 gcttgccggc cttctggatt atgaaactgg cgcgcgtgaa atgccgcaaa tgtcggccgc
      541 gaaccttaag cgcgcgctgg agcgtgcggg cgtcatcttc tcggaagatg aatccggcgt
      601 cagcctgctg ccgccgccgg aggttgtacc gctcgaaaag ctcaattcga gcaacgacga
      661 ataattagct cgccgctttg gctgtgaatt tggcgagaac tttcgtcaat ccctcgcgcg
      721 cgggctccgt catggcgcca agatcgtagc gcgcggcaag ccaatttatg tcgttgtatg
      781 tgagactaca gttgccggcc tcgtcttccc aggcgaggac gcgcaacggt agatcgagcc
      841 cggccatttg attggcctgc atcagcggcg tgccgccttt cggactgccg aaaataatta
      901 cttcggtggg gcgcaacgcg aggccaacag cggcggcccc gccagcgtga tcgacacgtg
      961 caaaaattgt gatcccggcc gctttcagtg ccgcctctaa tcggtcgagc gtttccctga
     1021 ccgaataatt gctcggaacc gtgatcaatc cattcgaaga cagtccgttt agcgacatcg
     1081 gcgatcctca aacttgtgcc caaggcatcc gggtaaaacg ctgggcgaga aaatccatca
     1141 agacttgcac gcgcacgggc cgcatcgggc tcggcggcgt gacgagatgc aaggctgccg
     1201 gcggtgccga ccaatctttt agcacagcct caagccggcc ctcggcaata tcgcgccaga
     1261 ccagaaattc cggctgcagg gcgaggccgg cgccggcgca gagcgacggc tgcaggacat
     1321 ccgcattatt ggcgcggatc ggccccgaca cggcgacgct gtattcgcct tgttttggat
     1381 ggctgaaatg ccagacgtcg cggctgcgcg aatagctata gagcagcgca atatgcgcgg
     1441 cgagatcttt gggatgctcg gggcgaccat gctggccgaa atacgcgggt gcgccgacga
     1501 gcagaacgcg cacctggcaa agccggcggg cgagcagact ggaatccggc aggctcgaaa
     1561 tccgcaatgc gagatcaaat ccctcgctga caatatcgac gatctgatcg ctgagactca
     1621 ggtccaccga aacgtccggg cagagtttga aaaagtccgg cagtagcggc gcgagatgga
     1681 cgatgccgaa agtcatcggc gcggcaacgc gcaccaatcc gcgcggcacc gccgattgcg
     1741 ccgtcgcctc ggcctcgacg gcttcaccct cggctagaat tcgactcgcc cgctcgaggg
     1801 tcgtgcgccc ggcctcactt agcgacatgc ggcgcgaggt acggtgaaaa agcgtcgtcc
     1861 cgagccgcgc ctcgagccgt ccgacgactt tcgagaccgt cgccttcgaa aggccaagct
     1921 cagacgcggc ctgtgcgaag gacccgagct ccgccacttt ggcgaagacc gcccaggcct
     1981 cgaaatccgg aagcttcgac atatcaaaac cagaaacagt acatttccat catttctatt
     2041 tcagatctga tggcccaagg caatattggt ctcaacaagc ggccgcatcc gaccgcaaga
     2101 ggatcaaaac gatgattgaa cgcagacctt tcgactcgct cggcggtgcc aagcacggtt
     2161 ggctcgatgc caagcatcac ttttcctttg gcgattatgg tgatgccaag cgcatgggct
     2221 ggggcgcttt gcgcgtctgg aatgacgaca cgatcgcacc gcagaccggc tttccgccgc
     2281 atccgcatcg cgacatggaa atcatcacct atgtccgtga gggtgcgatc acgcaccagg
     2341 acaatcttgg caacaaaggc cgcacggagg ccggcgatgt ccaggcgatg agcgccggca
     2401 ccggcatccg ccacgccgaa tataatctcg aaccggcgac gacgcggatt ttccagatct
     2461 ggatcatgcc acgcgaacag ggtctcggcc cggcgtgggg cgccaagccc ttcccgaagg
     2521 acgatcgcgc cggccagttc gtgactctgg caagcggctt tgacgacgac aaggatgctt
     2581 tgccgatccg cgcagacgcg cgcgtcgtcg gcgcgaccct caaagccggg cagacggcga
     2641 attacccgat tggcgaaacg cgtcacgcct atctcgtccc agccaagggc agtgtcaaag
     2701 tcaatgacgt gacgctcgat gcacgcgacg gcgccgccgt ctacggcgag aaagtgctcc
     2761 acgtcactgc gcttgaagac gccgaagtcg ttttagtcga cgcagcgtaa cgcaaaagcg
     2821 gccgccacac gaaatggcgg ccgcgtatcg gctttatcaa acagcgagac aatcatggct
     2881 aaggttctgg tgctttatta ttccgcctac ggtcacatcg aaaaaatggc cgaggccgtc
     2941 gccgagggcg cacgcgagac gggcgccaag gtggatatca agcgcgtacc cgaaaccgtg
     3001 ccggtcgaaa tcgctcaggc ctcgcatttc aagctcgatc aggcggctcc ggttgcgacg
     3061 atcgccgacc tcgccgacta tgacgcgatc atcctcggca ccggcacgcg tttcggccgc
     3121 ctctcctcgc agatggcgag tttcctcgat caggctggtg gcctttgggc gcgcggcgcg
     3181 ctgaactgca aggtcggtgc ggccttttcg tcgacagcga ctcagcatgg cggccaggag
     3241 accac
//
LOCUS       DASWOG010000394         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_174030,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000394 DASWOG010000000
VERSION     DASWOG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174030"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGB93_08215"
     CDS             complement(<1..393)
                     /locus_tag="VGB93_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0146124.1"
                     /translation="MMQPAFSWQTWAGLSAVFAALTAILAKLGVENINSNFATLIRTI
                     FILILAAIIVSVTRAWQAPSSISPRSFLFLALSALATGASWLCYFRALQLGQASKVAP
                     VDKLSVVLVALFGVTFLGEKLSAMNWLGV"
     gene            complement(390..1085)
                     /locus_tag="VGB93_08220"
     CDS             complement(390..1085)
                     /locus_tag="VGB93_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0146125.1"
                     /translation="MGFRLDTQADLKKGVARLVALDKRFAPIVAATGVPGLRRRESGF
                     AGIVAIVCAQQLSTASANAIWERLRAAFDPITPAAIAKARADRLARLGLSAAKIKTLK
                     SLAREIVAARFDLDALADEDAEVAHAKLTALHGIGPWTADVYLLFCLGHPDAWPAGDL
                     AVQEAVKLGLGLPIRPTTKEMMVLAEAWRPWRGVAAHVWWAYYRTVKQREGILPTGPA
                     AKAARRQAARVEV"
     gene            1438..>1888
                     /locus_tag="VGB93_08225"
     CDS             1438..>1888
                     /locus_tag="VGB93_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY0146126.1"
                     /translation="MEDSILPDAQDEQFQFTIPALQERRTRTLKHGDTFAMFDDNGDV
                     LSSLGSPEGIFHRDTRHLSHLSLSIFGQRPILLSSTLRDDNGALICDLTNPDLHKGDH
                     IALEHDLIHLRRLKFVWQNANFERLAVRNFSERRERVKIQIDFAADFA"
BASE COUNT          425 a          585 c          530 g          348 t
ORIGIN      
        1 gaccccaagc caattcatgg cggaaagctt ttcgccgaga aatgtgacac caaaaagcgc
       61 gacgaggacg acactcagtt tatcgaccgg cgcgactttg gaagcctggc ccaattgaag
      121 cgcacgaaaa tagcagagcc atgaggcgcc cgtcgccaag gccgacaacg ccagaaacag
      181 gaagcttcgc ggtgaaatcg aactcggcgc ctgccatgcc cgcgtgacgg aaacgatgat
      241 cgcggcgagg atcaggatga agatcgtgcg gatcagcgtc gcgaaatttg aattgatgtt
      301 ctcgacgccg agtttcgcca aaatcgccgt caacgcggcg aaaaccgctg aaaggccagc
      361 ccaggtctgc caggagaatg ccggttgcat catacctcta cgcgcgctgc ttgccggcgc
      421 gccgctttgg cggcgggacc agtaggaagg attccttcgc gctgtttaac ggtcctgtaa
      481 taggcccacc agacatgcgc cgcgacgccg cgccatggcc gccaagcttc tgcgagcacc
      541 atcatttcct tggttgtggg acggatggga agccccaatc cgagcttgac cgcctcctgc
      601 accgcgaggt cgcccgccgg ccatgcatct ggatggccga gacaaaacag cagatagaca
      661 tccgctgtcc aaggtccgat cccatgtagc gccgtcaact ttgcgtgcgc gacttcggcg
      721 tcctcgtcgg cgagggcatc gagatcgaag cgcgcggcga caatctcgcg cgcaaggctc
      781 ttcaacgtct tgatcttcgc ggccgagagc ccgagccgcg ccaaccgatc ggcacgcgct
      841 ttggcgatgg ccgctggcgt gatcggatcg aaggccgctc gcaaacgctc ccagattgcg
      901 ttggcgctgg cggtcgaaag ctgctgcgca cagacaatcg cgacaatccc cgcgaaaccg
      961 gactcacggc ggcgcagccc cggcacgccc gtcgccgcga cgatcggcgc gaaacgctta
     1021 tccagcgcaa cgagacgcgc gacgcccttc ttcagatcag cctgggtatc gagacggaac
     1081 cccatattgc aaagctctca cgcccgagcc cgcatttcaa caatcggcaa tgccgcaatg
     1141 acagggcaca tcccgcactc acctgttggc atctttgcga gcgccaaaaa atagttgcaa
     1201 tccgctccga aggtcgcact tacagattga tatcgtcata tacgcaagct atccgcaata
     1261 atctgcctgg tccgacgagc ctgtcgaaaa cggcctcgcg agcaacaata cttcagttca
     1321 agcttcagac ataaaaactc cgggcggctt ccttccggac gtgtcgaaat cttggttagc
     1381 tggatatctt aagacagaga ttccgctagc gccttcggaa ataaaggccg acgtatcatg
     1441 gaagacagca ttctgccgga tgctcaagac gagcaattcc agttcacaat tcccgcgctt
     1501 caggagcgcc gcacgcgcac gctgaagcat ggcgacacgt tcgcaatgtt cgatgacaat
     1561 ggcgatgtac tctcctcgct cggcagcccc gaaggcatct ttcatcgtga cacgcggcat
     1621 ctgtcgcatc tgagtctgtc gatctttggt cagcggccga tcctgctgag ctcgacattg
     1681 cgcgacgata atggcgcgct gatctgcgac ctgacgaatc ccgatctgca caagggcgat
     1741 catatcgccc tggaacatga tctcatccat ctgcggcggc tgaaattcgt gtggcagaac
     1801 gcaaactttg agcggctggc ggtccgcaat ttcagcgagc ggcgcgagcg cgttaaaatc
     1861 cagatcgatt ttgccgccga tttcgcgg
//
LOCUS       DASWOG010000395         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_283867,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000395 DASWOG010000000
VERSION     DASWOG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283867"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGB93_08230"
     CDS             complement(<1..255)
                     /locus_tag="VGB93_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146127.1"
                     /translation="MQAAWASRSEVETESVFGHDKSGGLHFRDFGNSGRAHMGNSDTF
                     EFWATSFVGIGGSIVGGLIARIFSRPRDGAFFHPAGFFLSI"
     gene            281..847
                     /locus_tag="VGB93_08235"
     CDS             281..847
                     /locus_tag="VGB93_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY0146128.1"
                     /translation="MPEPLRLPQEPRPVLRVSTSARLVLAGQAPGTRVNNTGIPYNDP
                     SGDRLRAWMNVSREAFYDESRFAIVPMGFCFPGLNAAKADLPPRRECRELWHDELFAL
                     MPQVTCVVAIGRYAQDYHLARAGYAVPKGALLADIVGEWESHARLTPRIIALPHPSWR
                     NNAWITRNPWFEKNVLPLLRAEVAKALA"
     gene            complement(844..1536)
                     /locus_tag="VGB93_08240"
     CDS             complement(844..1536)
                     /locus_tag="VGB93_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0146129.1"
                     /translation="MSPALSNFVVPILEIIWIDVLLSGDNAVVIALACAGLPKEQRLR
                     GIILGTAVAIGLRMIFTLLFADLLGLPFIKLFGGLLLLWIAVKLARDERSKKAIVEQT
                     SIFAAVRVIAVADAVMSLDNVIAIAAASKGSPLLILFGLGLSIPLIIFGSSLVLGLIN
                     RFPMLIWLGAAMLGFIAGQMIAHEFAAGMWPLPHWAIVQWSYFTLVCGVGGAAFVILI
                     ALVLPQKEKEMA"
     gene            complement(1533..2225)
                     /locus_tag="VGB93_08245"
     CDS             complement(1533..2225)
                     /locus_tag="VGB93_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146130.1"
                     /translation="MSRILVTGFGPFPGVKDNPSAALMTALRARRGHFELLSIHLETH
                     ILPVVYHGLAARLAALTVGTAPDAILHFGVASRRKIISVETRARNHANCAAPDAQGDR
                     PPSDMLDGKSEESLAVRIPAAGIAARIRAARIAAETSRDAGAYLCNATFYETLRSRKN
                     LPAGFIHVPLPAGPINASQVSFDQIVEAAEIAIVALVLHVRQVNSPATSPPDNTHKSA
                     STRVIRRAKAVA"
BASE COUNT          497 a          670 c          700 g          430 t
ORIGIN      
        1 gatcgaaaga aagaagccgg ctggatggaa gaacgcgcca tccctgggcc gggagaagat
       61 gcgggcgatc aatccgccca cgatggaacc gccgatcccc acgaatgagg tggcccagaa
      121 ttcaaacgtg tcgctattac ccatatgggc ccgtccgcta ttgccaaaat ccctgaaatg
      181 caggccgccg gacttatcgt ggccaaagac ggactcagtc tcaacctctg accttgatgc
      241 ccacgcggcc tgcattcgcg cctgccgtta ttgccgcgat ttgcctgaac cgctgcgcct
      301 gccgcaggag ccgaggcccg tgttgcgcgt ctcgacgagc gcgcggctgg tgctcgccgg
      361 gcaagcgccc ggcacacgcg tcaacaacac cggcattcct tacaacgatc cgtccggcga
      421 tcggcttcgc gcctggatga atgtctcgcg cgaggctttc tacgacgagt ctcgttttgc
      481 cattgtgccg atggggtttt gttttcccgg acttaacgct gcgaaggcag atctgccgcc
      541 gcgtcgcgag tgccgcgaac tttggcacga cgagcttttt gcgctcatgc cacaggtcac
      601 ttgcgtcgtg gcgatcggcc gctacgcgca ggactatcat ctggcgcggg cagggtatgc
      661 ggtgccgaaa ggtgcattgc tcgccgatat cgtcggcgaa tgggagagtc atgcacgctt
      721 gacgccgcgg atcatcgcgc tgccgcatcc gtcatggcgt aacaatgcct ggattacgcg
      781 caatccgtgg ttcgaaaaaa atgtcttgcc gctgttgcgc gctgaagtgg cgaaggcact
      841 tgcctaggcc atttcttttt ctttttgtgg cagcacgagc gcgatcagta tgacgaacgc
      901 ggcccctccg actccgcaaa ccagggtgaa ataggaccat tgcacaatgg cccaatgcgg
      961 caatggccac atgccggcgg cgaactcatg ggcgatcatc tggccggcga tgaagccaag
     1021 catcgccgcg cccagccaaa tgagcattgg aaagcggttg attaatccca gcacgaggct
     1081 cgatccgaaa atgatgagcg ggatcgagag cccaagtccg aagagaatca ggagcggcga
     1141 acccttcgac gcggccgcga ttgcgatgac attatcgagc gacatcacag catcggcgac
     1201 agcaatgact cgcactgcgg caaagatgga cgtctgctcg acaatcgcct ttttgcttcg
     1261 ctcgtcacgc gcaagcttga cggcgatcca gagcagcagc aggccaccaa acaattttat
     1321 gaatggcagg ccaagcaggt ctgcaaagag gagtgtgaag atcatgcgga ggccgatggc
     1381 cacagccgtg ccgaggatta tacctctgag gcgttgctct ttcggcagac cggcgcaggc
     1441 gagcgcgatg acgaccgcgt tgtcgccaga caaaagcacg tcgatccaaa taatttcgag
     1501 gatcggaacg acgaaattcg aaagggcagg actcatgcca ccgctttcgc gcggcgaatt
     1561 acgcgggtgc tggcggattt atgcgtattg tccggcgggg aggtggcggg tgaattcact
     1621 tggcgaacat gcagcacgag cgcaacaatc gcaatctccg cggcctcgac aatctgatcg
     1681 aagctgacct gcgatgcgtt tatcggcccc gccggcagcg ggacatgaat aaaaccggct
     1741 ggcagattct ttcggcttcg caatgtctca taaaacgtcg cattgcataa ataagccccg
     1801 gcatcgcgcg acgtctcagc cgcaatcctg gcggcgcgaa tacgggcggc aattccggcc
     1861 gccgggattc gtacagcgag gctttcttcg cttttgccat cgagcatgtc ggacggcggc
     1921 ctgtcgcctt gcgcatccgg tgcggcgcaa ttggcatgat tgcgggcgcg ggtctcaacc
     1981 gaaataatct tccggcggga ggcgacgccg aaatgcagga tagcgtcggg ggcggtgcca
     2041 accgtcaacg ccgcgagccg cgctgcgaga ccgtgataaa cgactggcag aatatgcgtt
     2101 tcaaggtgga tgctcaatag ctcgaaatgg ccgcggcgcg ctcgcaaagc cgtcatgagc
     2161 gccgctgagg gattgtcttt cacgccggga aacggaccaa agcccgtgac gagaatcctg
     2221 ctcatcggtg caaacgcccc gcaaggtcat gttttggaaa ggctgtctgc aaaaaagcgc
     2281 ggcccatgtg ccagaag
//
LOCUS       DASWOG010000396         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_9264, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000396 DASWOG010000000
VERSION     DASWOG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9264"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGB93_08250"
     CDS             <1..501
                     /locus_tag="VGB93_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0146131.1"
                     /translation="NRIADTAGVSIGSLYQYYPFKEALVAAVIDRHYRQITRRGRTVL
                     TEIASLPIERAVRRLVAFAIEAHRIDPRLHRVLAEQIPRVGRLENVEALNREGYALFR
                     DYLESRRDELRVRDLDLAAFVCATSIEALTHSAVLYHTEMLRDAEALIEETTRFISGY
                     LQPSPL"
     gene            complement(526..1284)
                     /locus_tag="VGB93_08255"
     CDS             complement(526..1284)
                     /locus_tag="VGB93_08255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0146132.1"
                     /translation="MRQRTKYGIDAPRVVRAYIVFGILLALPALIDLAWPGSSPIPVP
                     WSIGAALFAVLLLAWAAVMLRSSLVGKMRVRDRLVDALDLSGSERVLDAGCGRGLALI
                     GCAKKLTTGKAVGIDLWAGHLSGNNPEAARANAVVESVADRVEIETGDITRLPFSDAA
                     FDAVISMTVIHNIPSQEGRDQVVREIIRVLKPGGRIAIFDLLHTGRHAAILRQAGLSV
                     ESLGVDLLWLWPCRSLLARKPEAGGFQGLVTASS"
     gene            complement(2297..2731)
                     /locus_tag="VGB93_08260"
     CDS             complement(2297..2731)
                     /locus_tag="VGB93_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY0146133.1"
                     /translation="MTYNPLTDRNVAHAEIDDQSIRLLISTFYSQAREDELIGPIFAA
                     AVVDWDEHIENIANFWSSVMLRTTRYKGRPLRPHLILPLKGEHFDRWLQLFESTAREL
                     FAEDVAEAFVVRARRIADSFELAIGTQRGEIRQPRHSVKSSI"
BASE COUNT          640 a          975 c          948 g          616 t
ORIGIN      
        1 aaccggattg ccgacacggc cggcgttagc atcggctcgc tctaccaata ctatcctttc
       61 aaggaagcgc tcgttgccgc cgtcatcgat cgtcactatc gccagatcac gcggcgtggg
      121 cgcacggtgc tgaccgagat cgcttcgctg ccgatcgaaa gggccgtgcg aaggcttgtc
      181 gccttcgcga tagaggccca ccgcatcgac cccaggctgc atcgcgtgct tgcggagcag
      241 attccgcgcg ttgggcggct ggagaatgtg gaagcgctca accgcgaagg ctacgccctc
      301 ttccgggact atctcgaaag ccgccgtgac gaattgcgtg ttagggacct cgatctggcg
      361 gcatttgtgt gtgcgacatc gatcgaggcg ctgacccatt cggccgtcct ttaccatacc
      421 gaaatgctgc gggacgccga ggcgctcatc gaggaaacca cacgcttcat atctggatat
      481 ttgcaaccgt cgccgctttg atgccgcaat attccggcgg cggggtcatg atgacgccgt
      541 gaccaggcct tgaaaaccac cggcttccgg cttgcgcgca agtaacgaac ggcagggcca
      601 aagccacagc agatcgacgc ccaggctctc gaccgacaga cccgcctggc gcaatattgc
      661 cgcatggcgg cccgtgtgca ggagatcgaa gatcgcgatg cggccgcccg gcttcagcac
      721 gcggatgatt tcgcgcacca cttggtcgcg cccctcttgc gaagggatat tgtggattac
      781 ggtcatcgag atgacggcgt cgaacgccgc gtcggagaag ggcagccgcg tgatatcccc
      841 agtttcgatc tccacccggt cggcgacgct ttccacgacc gcgttggccc gcgccgcctc
      901 cgggttgttg ccggaaaggt gcccggccca aagatcgatg ccgacggcct tgcccgtggt
      961 gagcttcttg gcgcaaccga tcagggctaa gccccggccg catccggcat ctagcacgcg
     1021 ttcgcttccc gaaaggtcaa gcgcatcaac caggcggtca cggacccgca tcttacccac
     1081 aaggctggac ctgagcatga cggccgccca agcaagcaga agcaccgcga acaaggcggc
     1141 gccgatactc cacggcacgg gaatgggact tgatccgggc cacgcgagat cgatcagcgc
     1201 tggcaatgcc agcaagatgc cgaaaacgat gtacgcgcga accactcgcg gcgcgtcgat
     1261 gccgtatttt gtgcgctgtc tcatgcgttc gaatctcccg gcttcggcgg gggccgcctc
     1321 gcgcttcccg gtttcggcgg gggcgcggtg tcaattccgc ccatgggaaa gatatgagct
     1381 atcgctcacg ttgcatactc gcattgccag ggcgcagccc cggcagcgcg aacgcttggc
     1441 aaataccgga agaaaagcgt cccggaagcg atcgcgcctg atcgcggacg ccggtgccga
     1501 agcgactact cgcattttgg gtgcggatac ctcgcgacca gccatgtcag ggcggtgtcg
     1561 cgcgccaagg cgcgggggcg tccaccgcga cgtggcgaac cgcgcgatag aaatagctta
     1621 gtggatcagg gattagttcg ggtcttacgc acgctggagc ttgcaccaag cttcttgcac
     1681 taagtcctca gcctgggacg gatgggcgac gatagttcgc gcgtagcggg tgaacgccgc
     1741 gcgatggatc gtatggcgtc gagcgatctg ttcgtatttc accgcacccg acaccttcac
     1801 tgacggaatg cgccaggaac accgatctcc cggctaaaga gcgatcctcc gactggcatc
     1861 aggtatcaat tgagagcgga atagcgcgga cgcgctgatg ctccgtatca aacccggcaa
     1921 gtgggggcag cacaatcgag ggtatcttca atgaatatgc agacctcggc ttcatccgca
     1981 agtcgtgcat tttgaagtct ttcggaggtc aacaagcggt tgcacgcaag cggcttctgg
     2041 gtcggtcagg tttccgacca tgaaagcaag cggttgtttg atgctgtagc tacccgttat
     2101 aaaccgcagc atgccatctg gacccatata ggtacttggg cgcggcgatg acccgttccc
     2161 gcgaccgacc agtggaggtc gcgacggcgc gcgattgaac tcgtgcttct gtgaattcct
     2221 atttatttga tcgccctacc gctcgggtcc acggaccggc tactctggta aagcacaacg
     2281 ctaccctgcg gcgcactcag atcgaagact tcacgctgtg acgtggctga cggatttcgc
     2341 cgcgttgtgt gccaatcgcc agctcgaaac tgtcggcaat ccttcgcgcc cgcacgacaa
     2401 aggcttcggc gacatcttcc gcgaagagct cgcgcgccgt cgactcaaag agttgcaacc
     2461 agcgatcgaa atgttcgccc ttgagcggca aaatcaaatg gggccgcagc ggccggcctt
     2521 tatagcgtgt cgtgcggagc atcaccgagg accagaaatt cgcgatgttc tcgatatgct
     2581 cgtcccaatc cacgacggct gcggcaaaga tcggcccgat gagctcatct tcgcgggctt
     2641 ggctgtagaa tgtactgatc aataacctga tcgattgatc gtcaatctcc gcatgggcaa
     2701 cgtttcgatc tgtcaacgga ttgtatgtca tgatccgcac cgtttacgac gcggctcgcg
     2761 cgacagcaat tgtggtccgt tgtcgctctc gtttttggga acccgttacg agtccactcg
     2821 acgccggggc atacggcgga tcatgcctcg atcgtccttg aaagtgctag tgcccacgca
     2881 atctttgcgg gagacatcgc ccatcagccg atccaggtcc ttgagccgca gttgctgtct
     2941 gtcttcgaca ccgagcgcga tcgaactctg cgttcgcggc gatgggccca gtgatgttca
     3001 gttcgcactt tcctgcaaca tccgcaggcc aagttacccg gatgggcaac gcctatagat
     3061 ggagattcac ataggcggat caccgcacga cgatgatcgc ggacgccacc tatacgttcg
     3121 ccgctcaaaa cccgcgacgg agaacgggcc ggcaagccct tggcagctat ctgaaaaag
//
LOCUS       DASWOG010000397         4817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_101092,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000397 DASWOG010000000
VERSION     DASWOG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101092"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGB93_08265"
     CDS             <1..547
                     /locus_tag="VGB93_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0146134.1"
                     /translation="AERREFEPQAMRAFDGWLVKPLRLRSLLARIGTDAPHSTPPQES
                     PTPADLSGSRILLAEDNDINALILQKHLEKCGATLARVSDGLAALACVQDALTATPFE
                     AIILDLRMPGLDGLTLAREIRHLEATAHATPTRLIAVSADAFAETRRLALAAGVDAFL
                     TKPVDFAALDAALTTPREISC"
     gene            complement(537..1283)
                     /locus_tag="VGB93_08270"
     CDS             complement(537..1283)
                     /locus_tag="VGB93_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0146135.1"
                     /translation="MAGHSQFKNIMHKKGKQDAIRSKLFSELAREITVAAKLGLPDPA
                     MNARLRAAVIEARAENMPKDNIERAIKKAAAADGENYDEVRYEGYAPGGIAVIVEALT
                     DNRNRTAGEVRSYFSKAGGSLAETGAVSFMFDHVGAVEFPKKVASEDAMLEAAIDAGA
                     EDVVTTEENHQVTTTLEMMRDVAQNLETKFGEPSKVGFIWKPQTMIAVDDETGEKILR
                     LIDSLEDNDDVQNVYANFEISDALMAKLSS"
     gene            complement(1383..2000)
                     /locus_tag="VGB93_08275"
     CDS             complement(1383..2000)
                     /locus_tag="VGB93_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0146136.1"
                     /translation="MSETGLSANPRLQPTLRLVIALNLVFFVVEFIVAAKIGAVSLFA
                     DSVDFLEDTAMSMLVLAAMRLSPLTRARIGFALAGVLLLPVLAFFVTLGHKFFVAVPQ
                     APEPAPFALTGLAALVVNASCALLLARVRNAAGSLTRAAFLSARNDMVANIAIMVAAG
                     VTALRPSIWPDIVVGLSIAVLNADAAKEIWQAARREHGAALADDD"
     gene            complement(1997..2704)
                     /locus_tag="VGB93_08280"
     CDS             complement(1997..2704)
                     /locus_tag="VGB93_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0146137.1"
                     /translation="MRRQFLAATFVCAMFAGAASAEQAVIVPSVHDFAAMLPSPPTPG
                     SASEKDELVILMNIQNHRSPSDIARAQADAANRNIFLFASIFGAKFNAKDLPATAALS
                     NIVEADEKADIEPVKKLVPRLRPFAVDKDLHPVCPVKKTDDSYPSGHTMSGYLEALTL
                     ASILPQEKDTILARANEYAENRLVCGVHHPSDLAAGKLLAYALFPILAEDPHFQAARN
                     AVQVELRKALNFQSAVQ"
     gene            complement(2701..3525)
                     /locus_tag="VGB93_08285"
     CDS             complement(2701..3525)
                     /locus_tag="VGB93_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY0146138.1"
                     /translation="MRLLFIGDVVGRAGRLALTETLPGLRRDWSLDCVVVNGENAAGG
                     FGITEAILGDFLAAGADCVTLGNHSFDQREALSFIAHQPRLIRPANFPPGTPGAGATL
                     IETAAGARVLVVNLMGRLFMDALDDPFAVGAREIAACPLSEACDAAIVDFHAEATSEK
                     QAFAHFMDGRVSLVVGTHTHVPTADHQILPQGTAYQTDAGMTGDYDSVIGMEKEEPVR
                     RFITKVPGNRLEPAAGAATLCGLAVETNTYGHAIKVAPVRVGGRLSQARPDFWETK"
     gene            complement(3554..3925)
                     /locus_tag="VGB93_08290"
     CDS             complement(3554..3925)
                     /locus_tag="VGB93_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY0146139.1"
                     /translation="MLSIPPRLLPILLLTCSNVFMTFAWYGHLKFKDKPLWLVILLSW
                     MIALAEYCFAVPANRYGSAVYDGAELKAMQEVITLVVFAVFSVLYLGQKLTLNHLVGF
                     GFIALGAFFVFKGPFDGWRLG"
     gene            complement(3947..4522)
                     /locus_tag="VGB93_08295"
     CDS             complement(3947..4522)
                     /locus_tag="VGB93_08295"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0146140.1"
                     /translation="MTDASSKAALRAEALARRSRVTEAEAKAFAVHLGEAGGAFAADR
                     GAKIASVFWAINDEVATLPLLEGLAALGITIALPVMRGFGKPLEFRAWAPGAALNEAK
                     WGIMEPPEAPEVFPDLVFIPLAAFDRAGNRLGYGAGFYDRTLARLRAKQQIAAVGVGY
                     AVQEFPTIPAEVTDEKLDYVLTDREWIVCGS"
     gene            complement(4556..4708)
                     /gene="ssrS"
                     /locus_tag="VGB93_08300"
     ncRNA           complement(4556..4708)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGB93_08300"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          964 a         1508 c         1468 g          877 t
ORIGIN      
        1 ggccgagcgc cgcgaattcg agccgcaggc gatgcgcgcg ttcgatggat ggctcgtcaa
       61 gccgctgcgc ctgcgctcgc tgctggcgcg gattggcacg gacgcgccgc acagcacgcc
      121 gccacaggaa agtccgacgc ccgctgattt gtccggctct cgaatcctgc ttgccgaaga
      181 caatgacatc aatgccttga tcctgcagaa gcacctggaa aaatgcggcg cgactctcgc
      241 gcgcgtcagc gatggactgg ccgcgttagc ttgcgtgcaa gacgcgctca ccgcaacacc
      301 atttgaggcc atcatcctcg atctacgcat gcccggcctc gacggcctta cgctggcgcg
      361 cgagattcgc catctcgaag ctacggccca cgcgacgcct acccgactta ttgccgtatc
      421 ggccgacgca ttcgcggaaa ccagacggct tgcgctcgcc gccggggtcg atgcgtttct
      481 gacaaagccc gtcgatttcg ccgcgctcga tgcggcgctg acgacgccgc gcgaaattag
      541 ctgctgagct tggccatcag cgcgtcggaa atctcaaagt tcgcgtagac gttctgcaca
      601 tcatcattgt cttcgagaga atcgatgagg cggagaatct tctcgccggt ttcgtcatcg
      661 accgcgatca tggtctgcgg tttccagatg aagccgactt tgctcggctc gccgaatttc
      721 gtttcgaggt tttgcgcgac atcgcgcatc atctcgagcg tcgtcgtgac ctgatgattt
      781 tcctcggtcg tgacgacatc ttcggcaccg gcgtcgatcg cggcttcgag catcgcatct
      841 tcggacgcga cctttttcgg aaattcgacc gcgcccacat gatcgaacat gaaggagacc
      901 gcgccagtct ccgccagaga gccacccgcc ttgctgaaat aggagcggac ttcgcccgcc
      961 gtgcggttac gattgtcggt cagcgcttcg acgatgacgg cgatgccacc cggcgcgtat
     1021 ccctcgtaac gcacttcatc gtagttctcc ccgtcagcgg cggcggcctt tttgatggcg
     1081 cgttcgatat tgtccttcgg catgttttcg gcacgggcct cgatcacggc cgcgcgaagc
     1141 cgggcattca tcgccgggtc cggcaggccg agcttggcgg cgacagtgat ttcgcgcgcg
     1201 agctccgaga agagcttgga acggatcgcg tcctgcttgc ccttcttgtg catgatattc
     1261 ttgaactgac tatgccctgc catgcggccc tcaagtcctg cctaaatctg ggttttcgcc
     1321 acggccaatt gccgtttcgg cggtctataa gctgccttcg gccgaaaata aaggaagagg
     1381 ccctagtcgt cgtccgccag cgccgcgccg tgctcccgcc gggccgcctg ccagatttcc
     1441 ttggccgcat cggcattcag cacggcgatg ctgaggccga cgacaatatc cggccagatg
     1501 ctcggccgca gcgccgtcac gcctgccgcc accatgatgg cgatattggc gaccatgtcg
     1561 ttccgggccg acaaaaaggc ggcccgtgtc aggctgcccg cagcgttgcg cacgcgcgcc
     1621 agcagcagcg cgcaactcgc gttaacgaca agcgccgcta gcccagtcaa tgcaaagggc
     1681 gccggctcgg gcgcctgcgg cacggccaca aaaaatttgt gcccaagcgt tacgaagaaa
     1741 gcgagcaccg gcagcagcag cacgccggcg agcgcgaagc ctatccgcgc ccgcgtcagc
     1801 ggcgagagcc gcatggcggc gagcaccagc atgctcattg cggtatcttc gaggaaatcg
     1861 acgctgtctg cgaagagcga gacggcacct attttcgctg cgacgatgaa ttcgacgacg
     1921 aagaaaacaa ggttcagcgc gatgacgagc cgcaacgtcg gttgcagccg cggattcgca
     1981 ctaaggcctg tctcgctcat tggaccgcgg actggaagtt tagagccttg cgcaattcta
     2041 cctgcaccgc attacgtgcg gcttgaaaat gcgggtcctc agcgaggatc ggaaacaatg
     2101 cataggcaag cagctttccg gcagcgagat cgcttggatg atgcacgccg cagacaagcc
     2161 ggttctcggc atactcgttc gcgcgcgcaa ggatcgtgtc tttctcctgc ggcaggattg
     2221 aagccaaagt cagagcctcg agatagccgc tcatcgtatg gccgctcgga taggaatcat
     2281 cggtcttttt gaccgggcag acgggatgca gatctttgtc gactgcgaag gggcgcaagc
     2341 gcggaacgag ttttttgacc ggctcgatat ccgccttttc gtccgcttcg acgatattcg
     2401 acagcgcagc agtcgccggc aaatccttgg cattaaattt cgcgccgaag atggacgcga
     2461 agagaaagat attccggttc gcggcatcgg cctgcgcacg cgcaatatcg ctcgggctgc
     2521 gatgattctg aatgttcatc agaatcacga gctcgtcttt ttcggatgcg ctgccgggcg
     2581 tcggcggcga tggcagcatg gcggcgaaat catgcacaga tgggacgatg accgcctgtt
     2641 cggcactcgc cgcccctgcg aacatcgcgc aaacgaaagt ggctgcaaga aattgacgtc
     2701 tcatttcgtc tcccaaaaat ccggacgtgc ctgcgacagc cggccaccga cgcgcaccgg
     2761 cgcaaccttg attgcatggc cgtatgtatt tgtctcgaca gcaaggccgc aaagcgtcgc
     2821 cgcaccggcc gcgggctcca gcctgttgcc cggcactttg gtgatgaacc ggcgcacagg
     2881 ctcttccttt tccatcccga tgaccgagtc gtaatcgccg gtcatgcccg cgtcggtctg
     2941 ataggccgtg ccttgcggca gaatttgatg gtcggctgtc ggcacatgcg tatgggtgcc
     3001 gacgacgagc gagacgcgac catccatgaa atgcgcgaag gcctgctttt cgctcgtggc
     3061 ctcggcgtgg aaatcgacga ttgccgcatc gcacgcctcg ctgagcggac aggctgcgat
     3121 ctcgcgcgcg cccaccgcga atggatcgtc gagcgcatcc atgaaaaggc ggcccataag
     3181 attgaccaca agcacgcgtg cgcccgccgc cgtttcgatg agcgtcgcgc cggcgccggg
     3241 cgtcccgggt ggaaagttcg ccgggcggat caggcgcggt tgatgcgcga tgaagctcaa
     3301 agcctcgcgc tgatcgaagg aatgattgcc aagcgtcacg caatcggcgc ccgcggcgag
     3361 aaaatcgccg aggatcgcct cggtgatccc gaacccgccg gccgcgttct cgccattgac
     3421 gacgacgcaa tcgagcgacc agtcccggcg caggccgggc aaggtttcgg tcagcgcaag
     3481 gcggccggcc cggccgacga catcgccgat aaaaagaagc cgcattcaga aatcccgctc
     3541 tggcggaaat atattagccg aggcgccagc cgtcgaacgg ccctttgaag acgaagaacg
     3601 cgccgagcgc aatgaagccg aagccgacga ggtgattgag ggtcaatttc tggccgagat
     3661 agagtaccga gaagacggca aaaaccacaa gcgtgatgac ttcctgcatc gccttcagtt
     3721 ccgcgccgtc ataaaccgcg ctgccatatc gattggcggg cacggcgaag caatattcgg
     3781 caagtgcgat catccagctc aggagaatca ccaaccagag cggcttatcc ttgaatttga
     3841 gatggccgta ccaggcgaag gtcatgaaga cgttggagca ggtcagcaaa aggatcggca
     3901 gcaggcgcgg cgggatgctc agcatgaagg gcctttcgtt cgacggctag gaaccgcaga
     3961 cgatccactc acgatcggtc agcacgtaat cgagcttttc atcggtcact tcggccggga
     4021 tcgttgggaa ctcttgcacg gcatagccaa cgccgacggc agcaatctgt tgctttgccc
     4081 gcagtcgcgc caaggtgcga tcgtagaagc cggcgccata gccaagccgg tttccggcgc
     4141 ggtcaaatgc ggccagcggg atgaagacaa gatcggggaa gacttccggc gcttccggcg
     4201 gctccatgat gccccatttg gcctcgttca aagcggcgcc cggcgcccat gcacgaaact
     4261 caagcggctt gccgaagccg cgcatcaccg gcaaagcgat ggtgatcccc agcgccgcga
     4321 gaccttcgag aagcggcagg gtcgcgactt cgtcgttgat ggcccagaag acggacgcga
     4381 tcttggcgcc gcggtcagcc gcgaacgcgc ccccggcctc gcccagatgc acggcgaagg
     4441 ctttggcctc cgcctctgtc acccggctcc gccgtgcaag agcctcggca cgcagcgcag
     4501 ctttggagga cgcgtcggtc aaggcagact ttaggacatt cgagattggg aaaagcgcgg
     4561 ggccacgatg gccgtgggat tcgatcccgg gtacctacaa agtaggtggg cgccgtgtac
     4621 cctagcccac gggctaagac agggccagct cccgtcgaga tcgataaggc cccggggaat
     4681 tagatcctaa cgcaccccgc agctccgcca aaccaatcta ggaccggcgg aggctgaata
     4741 ccagaggcaa atttaagctc gaatcaggct tgcgccggaa gcgcggcctt cgccgcctcg
     4801 ccgatcgcct ttaacgc
//
LOCUS       DASWOG010000398         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_266177,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000398 DASWOG010000000
VERSION     DASWOG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266177"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..833)
                     /locus_tag="VGB93_08305"
     CDS             complement(327..833)
                     /locus_tag="VGB93_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0146141.1"
                     /translation="MGLFTKDIKDMDDLFVHQLKDIYYAENRIVVTLPKMIEKATSAE
                     LKQGFQTHLAETKNHVKRLDKVFDLHGVSPESVKCPAIDGIIKEADEVAGEVDDKNVL
                     DAALINAAQAAEHYEIVRYGSLIAWAKQLGRNDCAGVLQKTLDEEKATDKKLNTLAES
                     KINLRAVS"
     gene            1118..2050
                     /locus_tag="VGB93_08310"
     CDS             1118..2050
                     /locus_tag="VGB93_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0146142.1"
                     /translation="MARRRIGQERFGFATDRDQRSSLDDLVKLIDWTPIDCALRVISC
                     AAKGEPAWPPLSLFKAMLLSIWYDLSDVKLAEALDDRGSFRRFCGFSVSEPTPERTAF
                     VRFRKALVGHGLDRILFEEITRQLKAKAIRVKTGTLVDATIIASTSEGDGEAHWVKHK
                     GRPAVHGFKAHVGADADTALVEELAVTPANVNDGRAGPDALPANPGEVFADSAYRGSH
                     FAAAVRAKGGTPRIVATGMWGRDEAETSARLHAWNRPIHRIRGRIEKIFGTWKRSYGL
                     RRMRWRGLAKAAVQIHFTAIAYNMKRTLTIAAKA"
BASE COUNT          412 a          586 c          600 g          465 t
ORIGIN      
        1 aacgccaaca atgcaagcag gtgatagctt ccgaccgcga acgcgtatcg ccagttgatc
       61 cgcttgagag ctgccccgac gggacatgac agggattcag cctggaagat cgcaagatcc
      121 gcgccggcgc ggccaacttc ctccatcgac tttcctgact ttcaatgaac atgtcttgta
      181 cacggcttcg gcgtattccg gcacgcaatg ttgcgaaatg acactaattt aacaatccag
      241 tggctgtgat ctgccctcgc cacacgcaaa acggcgcgcg gatccaccgc gcgacgtttt
      301 gtctcaaccc tgtttcggcc cgcagatcag cttacggcgc gaaggttgat cttgctttcg
      361 gccagcgtgt tcagcttctt gtcggtcgcc ttttcctcat cgagggtttt ctgcaagaca
      421 ccggcgcaat cgttgcggcc aagctgcttt gcccacgcga tcaggctgcc gtagcgcacg
      481 atctcgtaat gctcggccgc ctgggcggcg ttgatcagcg cggcgtcgag cacgttttta
      541 tcatcgacct cgccggcgac ttcatccgct tccttgataa tgccgtcgat cgccggacat
      601 tttaccgatt ccggtgagac accatgcaga tcgaaaacct tatcgagccg tttgacgtgg
      661 tttttcgttt cggcgagatg ggtttgaaag ccctgtttca gctccgccga tgtcgctttt
      721 tcgatcatct tcggcagcgt cacgacgatc cgattttctg cataatagat atccttgagc
      781 tgatggacga agagatcgtc catatctttg atatccttcg tgaagagacc catggaactt
      841 cctcctcgct gtgagtaagg ccgcgcggta ggcgcggaca tgggatggca acggcgacag
      901 gagccaggtg ttccaggaaa agaatcgtcg cctgggtcgc tggcgcagcg caagcgttgc
      961 atagcgagcg cctagtctgt cgaccataat ggcggcaaat atccgtgatg gaacatgcca
     1021 ttacgcttcg gagagtttga gagacctgtg cgcgggtcgc tggacgaggg ttggcattta
     1081 tgattccatt ggtctgaaca atggattgag gaatgcaatg gcacgtcggc gcattggtca
     1141 ggagagattt ggttttgcca ctgatcgcga ccaacgctcc tcgctggatg atcttgtcaa
     1201 actgatcgac tggacgccga tcgattgtgc cctgcgtgtg atttcctgtg ctgccaaggg
     1261 agagcccgcc tggccgccac tgtcgttgtt caaggcgatg ctgctttcaa tctggtacga
     1321 cttgtcagac gtcaagcttg ccgaggcgct ggatgacaga ggctcgttcc gacgcttctg
     1381 cggattttcg gtttcggagc cgacacccga gcgcacggct tttgtgcgtt tccgaaaggc
     1441 ccttgttggc catggcttgg acaggatcct gttcgaggaa atcactcgtc agctcaaggc
     1501 aaaggcgatc cgtgtgaaga ccggcacgct tgtcgatgcg acgatcattg cctcgaccag
     1561 cgagggtgac ggcgaagcgc attgggtcaa acacaagggc agaccggcgg ttcatggttt
     1621 caaggcccat gtcggcgccg atgccgatac tgcccttgtc gaggaacttg ccgtcacgcc
     1681 tgccaatgtc aacgatggca gggctggccc cgatgctctg ccggccaatc ccggcgaggt
     1741 gtttgccgac agtgcttatc gaggaagcca tttcgcggcg gccgtgcgtg cgaagggcgg
     1801 gacgcctcgt atcgtcgcca ccggcatgtg gggccgtgac gaagccgaaa catcggcacg
     1861 ccttcatgcc tggaaccggc ccattcaccg catccgcggg cggatcgaga agatcttcgg
     1921 gacctggaaa cgcagctacg gtcttcgacg catgcgatgg cgaggacttg ccaaagctgc
     1981 tgtgcaaatc catttcaccg ccatcgccta caatatgaaa cgcacgctca cgatcgccgc
     2041 caaggcttga atcaaaggct tga
//
LOCUS       DASWOG010000399         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_119091,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000399 DASWOG010000000
VERSION     DASWOG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119091"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGB93_08315"
     CDS             complement(<1..268)
                     /locus_tag="VGB93_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146143.1"
                     /translation="MPSTLVVINRYVRMRKSTLERISQIISDLTRANSKTAFVPRTMG
                     PPPKMINPQTPRQAFQEMLRQIFDVAQRSAASFEIIGLARLQKIP"
     gene            complement(300..968)
                     /locus_tag="VGB93_08320"
     CDS             complement(300..968)
                     /locus_tag="VGB93_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146144.1"
                     /translation="MRRVTANDFDFTSAPLFRKCATIPVANVVFAKGGEKVVTLQSGA
                     AGQFAETINIAQLGDAIVTRSAGDSYIPKKFSNNFEINPANSDEYRSKNFGCAIFQDE
                     DFVIMAPWGEDQTIAAGGVIFQSLAGDTPEVYGNQGRSFEADYAREAADGSLLPLTLP
                     LKTQLQWATDKGELAHVGDIRVRAVYARKQASQNSLGTSDEVIRMADIVLNRSSRADS
                     NSGT"
     gene            1114..1554
                     /locus_tag="VGB93_08325"
     CDS             1114..1554
                     /locus_tag="VGB93_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY0146145.1"
                     /translation="MLDLTLAIAHHLLVFSLFGIVFAEFFVLRPGLGGDALVRLARLD
                     LIYGIIAGLIVFVGFARAIFAAKGWHYYAHNGFFWAKIGTFALIGLASVPPTLAYLRW
                     RKAAAPPSDQEVKRVRFYLHLELALFVPLLGFAAAMARGYGEFG"
     gene            complement(1555..2382)
                     /locus_tag="VGB93_08330"
     CDS             complement(1555..2382)
                     /locus_tag="VGB93_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146146.1"
                     /translation="MHFRKAAVERRVAASAISHSIRNLETRVGVRLFHRTTRSVSLTD
                     AGARFLAELQPAFEQIDAALEGLNAFRGTPFGTVRLNIPNSIAPFVLQDVIGPLLQEN
                     PGLNLDIVATDRLVDIAKDGFDAGIRFGENLSQDMIAVRIKSRLRFAVVGSPDYFSGR
                     KKPRTPADLKDHSCIRYRFPSGAIFNWEFERGGELVHVEVDGPLTLDSQDLMVEAALQ
                     GCGLAFVFDYRVEPFLASGALIRCLEDWCTTDDSLFLYYPSRHYVSAGLRALIDKLK"
BASE COUNT          563 a          767 c          720 g          576 t
ORIGIN      
        1 caggtatctt ctgaagtcgt gccagcccta tgatctcaaa actagccgca ctcctctgag
       61 caacatcaaa tatctgccgc agcatttcct ggaacgcttg gcgtggggtt tgcggattta
      121 tcatcttggg aggcggcccc attgtccgcg gcacaaaagc tgtttttgaa tttgccctcg
      181 tgagatcgct aataatttgg gaaattcttt ccagtgtaga tttccgcatc ctcacatatc
      241 gattgataac caccaaggtg ctcggcattt tgctaatcaa gcgcggcggc gaaaggaaac
      301 taagttccgg aattagaatc agcccgcgaa gacctgttaa ggacaatatc ggccatgcgg
      361 atgacttcat ccgaagtgcc taaagagttc tgagacgctt gcttgcgcgc atagacggca
      421 cgcacacgaa tgtccccgac gtgcgcgagc tcgcccttgt cggtcgccca ttgcaattgc
      481 gttttgagcg gtaaagtgag cggcagcaat gagccatcgg cggcttccct cgcgtaatcg
      541 gcttcgaagc tgcgaccttg attgccgtag acctccggcg tgtcgcccgc gagactttga
      601 aagatgacgc cgcctgccgc gatcgtctgg tcttcacccc acggcgccat aatgacgaaa
      661 tcttcgtcct gaaagatcgc gcagccgaaa ttcttcgatc gatattcgtc ggaattggcc
      721 gggttgattt caaaattgtt gctgaacttc ttgggaatgt aggaatcgcc cgcgctgcgg
      781 gtcacaatgg cgtccccaag ctgtgcgata ttgatcgtct ccgcgaattg tccggccgct
      841 cccgattgga gcgtgacgac cttttctcct cccttcgcaa aaaccacatt cgcgaccggg
      901 atcgtcgcgc atttccggaa aagcggcgcg ctggtaaagt cgaaatcatt cgccgtgacg
      961 cgtctcaaat tgaacctctt ggcaaatttc aaaaatgaag ttcccagtaa gaatatacaa
     1021 ggaactcttt ccgaaccgtt gcgcggcagc acagcttggc tgtgtaatcg cacttcgcta
     1081 caaagccccg gacccgctgg ggaaataatt gccatgctcg atctcactct ggccatcgct
     1141 catcatcttc tcgtcttcag tctattcggc atcgtcttcg ccgaattttt tgtgttgcgg
     1201 ccgggcctcg gcggcgacgc gctggtgcgc ctcgcgaggc tcgatctcat ttacgggatc
     1261 atcgccggtt tgatcgtgtt cgtcggcttc gcccgcgcga tcttcgccgc caaaggctgg
     1321 cactattacg cccacaacgg attcttctgg gcgaagatcg gcacattcgc tctgatcggc
     1381 ttggcgtcgg ttccgcccac tctcgcctat ctgcgctggc gcaaggccgc tgcgccgccg
     1441 agcgatcaag aggtcaaacg cgtgcgcttt tacctccacc tcgaactcgc gctttttgtg
     1501 ccgctgcttg gcttcgccgc ggctatggcc cgcggctatg gcgaattcgg ctgatcactt
     1561 gagtttgtcg atcaacgcgc gcagtccagc cgagacgtag tggcggctcg gataataaag
     1621 aaacagactg tcgtccgtcg tgcaccaatc ctccaggcat cggatcaagg cgccggaggc
     1681 gagaaacggc tcgacacgat agtcgaaaac gaaagcaaga ccgcaacctt gcagcgccgc
     1741 ttcgaccatc agatcctggc tgtcgagcgt gagcggccca tcgacttcga catgaacgag
     1801 ttcgcccccg cgctcaaact cccaattgaa gatggcgccg ctcgggaaac gatagcggat
     1861 gcaggaatgg tccttgagat cggccggtgt gcgcggcttt ttgcggccac tgaaataatc
     1921 cggcgacccg acaacggcga agcgtagccg cgacttgatc cgaaccgcga tcatatcctg
     1981 acttagattc tcgccgaaac ggatgccggc atcgaaacca tccttggcga tgtcgacgag
     2041 cctgtcggtc gcgacaatat cgagattgag acccggattt tcctggagca atgggccgat
     2101 cacatcctgc aacacgaagg gcgcgatgga atttggaata ttgagccgca ctgtcccgaa
     2161 cggcgtgccg cgaaaggcgt tgaggccttc gagcgccgca tcgatctgct cgaacgccgg
     2221 ttgcaattcg gcgagaaacc gcgcgcccgc atccgtcagc gacacgctgc gcgtcgtgcg
     2281 atgaaagagc cggacgccaa cccgcgtttc aagattgcga atggaatggc tgatagccga
     2341 cgccgcgacc cggcgctcga cggccgcctt gcgaaagtgc atgggctgga aattgctgtt
     2401 ctaaattcat caccatagag atgattatgc atcttatcgc atttatccgc gggcgctaga
     2461 tcacgggcga gctagcgatc aagtcgccgt gtggcgcctg aaaagccgca caatcgtctc
     2521 taacttcgtg gcaaaagccc cagtcaaagg agttatcgat catgcaaaag cgcatcctag
     2581 gaaaaagcgg cctcgaagtc tcagcgctcg gcctcggctg tatggg
//
LOCUS       DASWOG010000400         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_63981,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000400 DASWOG010000000
VERSION     DASWOG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63981"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VGB93_08335"
     CDS             <1..938
                     /locus_tag="VGB93_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146147.1"
                     /translation="VSGGGKSTLIVDTLYKAVARKLNGSIEHPAPHKAIEGLQHLDKV
                     IDIDQSPIGRTPRSNPATYTGAFTPIREWFAGLPEAKARGYLPGRFSFNVKGGRCEAC
                     QGDGVIKIEMHFLPDVYVTCDVCKGSRYERETLEVKYRDKSIADVLNMTVEEAGALFK
                     AVPSIREKMETLSRVGLGYIKVGQQANTLSGGEAQRVKLAKELARRATGRTLYILDEP
                     TTGLHFHDVAKLLEVLHELVEGGNSVVVIEHNLDVIKTADWIIDLGPEGGVGGGEIVA
                     QGTPEAIVKVPQSHTGHYLKPILERRVTQRKDAAE"
     gene            complement(1308..>1731)
                     /locus_tag="VGB93_08340"
     CDS             complement(1308..>1731)
                     /locus_tag="VGB93_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY0146148.1"
                     /translation="RSLGTVPNYFEVERFNVNAERRHNAKGEYVTVSEAVVKMRIGDD
                     TSISVAEGNGPINALDLALRKDLGKYQSFVEDLELVDYRVRVFQGGTDAVTRVLIEFS
                     DGSGENWSTVGVSANIIDASFQALTDAITYKLLKAGAE"
BASE COUNT          358 a          494 c          517 g          362 t
ORIGIN      
        1 gcgtttccgg cggcggcaag tcgaccttga tcgtcgatac gctctacaaa gccgtggcgc
       61 gcaaactcaa cggctcgatc gagcatccgg cgccgcacaa ggcgatcgaa ggcctgcaac
      121 atctcgataa ggtgatcgac atcgatcaat cgccgatcgg ccgcacaccg cgctcgaatc
      181 ccgcgaccta taccggcgcc ttcacgccga tccgcgaatg gtttgccggc ctgccggagg
      241 ccaaggcgcg tggttatctg ccggggcggt tctcgttcaa cgtcaagggc ggccgctgcg
      301 aggcctgcca gggcgatggc gtcatcaaga ttgagatgca cttcctgccg gacgtctatg
      361 tgacctgcga tgtctgcaag gggtcgcgct atgagcgtga aacgctcgaa gtgaaatatc
      421 gcgacaagtc gattgccgac gtcctcaaca tgacggtcga ggaagccggc gcgctgttca
      481 aggcggtgcc gtcgatccgc gagaagatgg aaacgctgag ccgtgtgggc ctcggctata
      541 tcaaggtcgg ccagcaggcc aacacgctat cgggtggcga ggcacaacgg gtgaagctcg
      601 cgaaagagct ggcgcgccgc gcaaccgggc gcacgctcta tattctcgat gagccgacaa
      661 cgggcctgca cttccacgat gtggcgaaac tacttgaagt gctgcatgag cttgtcgagg
      721 gcggcaatag cgtcgtcgtg atcgagcaca atctcgatgt gatcaagacg gccgactgga
      781 tcatcgatct cggacccgag ggcggcgtgg gcggcggcga gatcgtcgcg caagggacgc
      841 ccgaagcgat cgtcaaagtc ccgcagagcc ataccggcca ttatctcaag ccgatcctcg
      901 agcgtcgcgt gacgcagcgg aaagatgcgg cggagtaggg agaagtagga agcctgcgac
      961 caaaatgccc gtcgtatgcg catttcgcat gtgtttattc aaattttcta tcgatcattg
     1021 gtatcgattg gcaaattgcc cccgaagcat cgtgtgacac ttgattacaa tagcgtcaca
     1081 ttcgctgaat gcatgcgttt aaggcataac agtgccgcga tctttaaact tctgctcgag
     1141 caaaaatcga tttactaacg tggtgacgct ggtgcacctt gagttattcc aaatttttct
     1201 cggtgtgtcc ggctttgccg cgagagctct attcccgcga ctcgcgtgcc aaaatgcccg
     1261 ccggatcctc ctcccatccg gcgggcgttt tctttttctg aatcgcttta ttccgcgccg
     1321 gctttcagca gtttgtaggt gattgcatcg gtgagcgcct gaaatgaggc gtcgatgatg
     1381 ttcgccgata cgccgaccgt cgaccagttt tcgccgctgc cgtcgctgaa ttcgatcagc
     1441 acacgtgtca cggcatccgt gccgccctgg aacacgcgga cgcgataatc gacaagttcg
     1501 aggtcctcga cgaaggattg atacttgccg aggtccttgc ggagcgcgag atcgagcgcg
     1561 ttgatcggac cgttgccttc ggcgactgag atcgatgtgt catcgccgat ccgcatttta
     1621 acgacggctt cggagacggt gacatattcg cccttggcgt tatggcggcg ctcggcattg
     1681 acgttgaaac gctcgacctc aaaataattc ggcaccgtgc caagactgcg c
//
LOCUS       DASWOG010000401         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_45345,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000401 DASWOG010000000
VERSION     DASWOG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45345"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGB93_08345"
     CDS             <1..877
                     /locus_tag="VGB93_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY0146149.1"
                     /translation="AAAETLEKVHQALIYRAHGEPTFEKLNALAEFLASIDIKIAKGQ
                     VLTPRQFNGILAQVKGTEHENIVNDVVLRTQAQAEYVAENYGHFGLHLCRYAHFTSPI
                     RRYADLIVHRGLIRALRLGNDGLPDMTGEQLAEIAAQISATERRAMAAERETVDRLIA
                     QFLSERVGATFQGRISGVTGAGLFVKLAETGADGFIPIATLGDDYFNHDESSHALIGR
                     RSGATHRLGDEIAVKLVEAAPFAGALRFELISGAGGARLRSVKTQGKRAGRRDNGSDG
                     PSTSARLKKLRKIKN"
     gene            903..1382
                     /locus_tag="VGB93_08350"
     CDS             903..1382
                     /locus_tag="VGB93_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY0146150.1"
                     /translation="MAIDTVNLSVVPLSGSKAAPDDSFARPKRNAWLSIRRGFSGHCP
                     SCGKGAIYGKYLKVNDRCTVCNTELFHQRADDAPPYLTILVVGHIIGAMMLSADTMFP
                     DFPIVYQMILWPALTVAMSLILLPAFKGALIGYQWALRMHGFDSAPDASSAGTVPNG"
     gene            1375..2091
                     /locus_tag="VGB93_08355"
     CDS             1375..2091
                     /locus_tag="VGB93_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0146151.1"
                     /translation="MADGVFAPTVSPARPVKPKAASSLILVDTTQSVPHVLMGRRNAS
                     LRFMPGKFVFPGGRVEREDRDITAADTLLDSVAARLSKRNRRDAPEPRTLALAAIRET
                     FEETGLVIGEKAAPSATQVPQSWRGVVGLGFLPKIGDLRFLARAVTPQHLPRRFDTRF
                     FIADAASIAYSVNGVAHADAELVELRWLPIAEALELDVGNITGSILREAAQRITAGLT
                     ADPPAAFFSYRNRRFSRDEL"
     gene            2088..>2449
                     /locus_tag="VGB93_08360"
     CDS             2088..>2449
                     /locus_tag="VGB93_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146152.1"
                     /translation="MKTASKTRVFVVAAAITTVMIAAIGLSLSATLIAVRLAQAGYSA
                     RAIGLHPAASSLVGLVTAVYVPWLAHIIGVRRLLISALLLGAVCLAGLALTHGYWPWL
                     AVRCVYGIALTILFIFAE"
BASE COUNT          431 a          797 c          724 g          497 t
ORIGIN      
        1 cgccgcggca gagacgttgg aaaaagtgca tcaggccctg atctatcgcg cgcatggcga
       61 gccgacattc gagaagctta atgcgctcgc tgaattcctc gcctcgatcg acatcaagat
      121 cgcgaagggc caagtgctga cgccgcgcca gttcaacggc atcctggcgc aagtgaaagg
      181 caccgagcac gagaacatcg tcaacgatgt cgtgctccgc acccaggcac aggcggagta
      241 tgttgcggag aactacggcc atttcggcct acatctgtgc cgctacgcgc atttcacctc
      301 gccgatccgc cgctatgccg atctcatcgt ccatcgcggc ctaatccgcg cgctgcggct
      361 tggcaacgac ggcctaccgg acatgacggg cgagcaattg gcggagattg cggctcaaat
      421 ttccgcgacc gagcggcgcg ccatggcggc ggagcgcgaa acggtcgacc gcctgatcgc
      481 gcaatttttg agcgaacgcg tcggcgccac gttccaaggg cgcatctccg gtgtcacggg
      541 cgcgggactt ttcgtcaaac tcgcggaaac cggcgccgac gggtttatcc cgattgcgac
      601 gctcggcgat gactatttca accatgacga gagttcgcat gcgttgattg gtcgccgcag
      661 cggcgcgacg catcgccttg gcgacgaaat tgcggtgaag ctcgtcgaag cggcgccttt
      721 tgcaggcgct ctgcggtttg aattgatatc cggcgcgggc ggcgcccgcc tacgcagcgt
      781 caaaacccag gggaagcgcg cgggacgccg cgacaacggg tctgatggtc cctctacgtc
      841 cgctcgttta aaaaaactga ggaagatcaa aaattaaatc gaatgacagc taggctcccg
      901 tcatggctat cgacacggta aacttgtccg ttgtgcccct ctccggcagc aaagccgcgc
      961 ccgacgatag cttcgcgcgg cccaagcgaa atgcatggct ctcaatccgg cgtgggtttt
     1021 ccggccattg cccctcgtgc ggcaagggcg cgatttacgg caaatatctg aaggtcaatg
     1081 accggtgcac ggtctgcaac accgaattgt ttcatcagcg ggctgacgac gcgccgccct
     1141 atctgacgat cctcgtcgtc ggccacatca tcggcgcgat gatgctttca gccgacacca
     1201 tgtttccgga ttttccaatc gtctatcaga tgatcctgtg gcccgcgcta accgttgcca
     1261 tgtcgctgat cctgctgccg gcgttcaagg gcgcccttat cggctatcaa tgggcgctgc
     1321 gcatgcacgg atttgattcc gcgccggacg cgtcttcggc aggcaccgtg ccgaatggct
     1381 gacggcgtct tcgcgccgac tgtctcaccc gcgcgacccg tcaagccaaa agcggcttcc
     1441 agtctcatcc ttgtcgatac gacccaatcc gttccgcacg tcctgatggg gcggcgcaat
     1501 gccagcctgc gtttcatgcc gggcaagttt gtcttccccg gcggccgcgt ggagcgcgaa
     1561 gatcgcgaca tcacggcggc cgatacgctc ctcgactccg ttgcggcccg attgagcaaa
     1621 cgcaaccgcc gcgatgcgcc ggagccccgc acgctagcct tggccgccat ccgcgaaaca
     1681 ttcgaggaga cgggcctcgt gattggcgag aaggccgcac cttcggcgac ccaggtgcct
     1741 caatcctggc gcggtgttgt cgggcttggc tttctgccga agatcggcga tttgcgcttt
     1801 ctcgctcgcg ccgtaacgcc gcaacatttg ccgcgccggt tcgatacgcg tttcttcatt
     1861 gccgatgcgg cgtcgatcgc ctacagcgtc aacggcgtag cgcatgcgga tgcagaactc
     1921 gtcgaacttc gctggctgcc gatcgccgaa gcactggaac ttgatgtcgg caacatcacc
     1981 ggttcgatct tgcgcgaggc agcgcaacgc attaccgcag ggctgactgc cgatccgcct
     2041 gcggcgttct tctcttatcg gaatcgccgc ttctcccgcg acgaactttg aaaacagcct
     2101 ccaaaacgcg cgtcttcgtt gtcgcagcgg ccatcacgac agtgatgatc gccgccatcg
     2161 gtctgtcgct gtcggcgact ttgatcgcgg tgcggctggc gcaggccggc tattccgcac
     2221 gcgctatcgg cctccatccc gccgcgagca gcctcgttgg cctcgtcacg gcggtttatg
     2281 tgccctggct cgcgcacatc atcggcgtcc ggcggctcct gatctccgca ctattgctgg
     2341 gcgccgtctg ccttgccggc cttgcgctca cgcacggcta ttggccctgg cttgcggtgc
     2401 ggtgcgtcta tggcatcgcg ctgaccattc ttttcatttt tgccgaatt
//
LOCUS       DASWOG010000402         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_321155,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000402 DASWOG010000000
VERSION     DASWOG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321155"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..938)
                     /locus_tag="VGB93_08365"
     CDS             complement(165..938)
                     /locus_tag="VGB93_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0146153.1"
                     /translation="MFDHSVIFPALAFLFAAGFCSGAMNSLAGGGSFVSVPAMIATGM
                     PAVLANTSSTIALWPGTIASTLIYPNSFRSICGVGKWPMLIVTLVGGLIGSLLLLHTP
                     SSSFTIMLPWLLLLATLALAFGRQIAIAIEGRFHGSPLVLVAQFILGIYGGYFGGAAS
                     VMMLAVWCLFGETDIKALQGPRSLLITAANTVAIIIFLFARAVLWPQTLAMLAGSLIG
                     GIGGAQLGRVTPSHLVRIVTLGITIAITCAFFIKTYWPH"
     gene            complement(988..1905)
                     /gene="murB"
                     /locus_tag="VGB93_08370"
     CDS             complement(988..1905)
                     /gene="murB"
                     /locus_tag="VGB93_08370"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0146154.1"
                     /translation="MIFPDLTPALHKAAPDLRGRLESNVRMADLCWFRTGGPAQVLFT
                     PADEEDLAYFLQRLDPDIHVTVVGVGSNLLVSDGGIAGIVVRLGKGFHNLSIDGLRIE
                     AGARILDVKLATSAAEAGIAGLAFYRGIPGAVGGALRMNAGAYGAETKDVLVSCRAID
                     RTGKLHIFSNSEMGFTYRHAAVPHDLIFTSALYEGKAGERAAIEAQMVEITKSRQETQ
                     PVNTRTGGSTFKNPPGHKAWQLVDAAGCRGLVVGDAQVSELHCNFLINRGAASAQDIE
                     SLGEEVRRRVFAASGVTLEWEIERVGAPQ"
     gene            complement(1902..>2533)
                     /locus_tag="VGB93_08375"
     CDS             complement(1902..>2533)
                     /locus_tag="VGB93_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0146155.1"
                     /translation="NVIIHDRLKGTEVRLENLGLPMPGHHNALNATAAIAVAYQLGMS
                     ADSIRSALAGFGGVKRRFTPTGEWHGVRIFDDYGHHPVEIAAVLRAARASTKGQVIAI
                     VQPHRYTRLNSLFNEFATCFNDADVVIVADVYAAGEATIEGADRDSLVAALRAHGHRQ
                     ALPLAGPTQLAEIVRSVAKADDYVVFLGAGNITQWAYALPGQLAAGDAA"
BASE COUNT          573 a          769 c          749 g          442 t
ORIGIN      
        1 cgaacggcag gctaccgaga agcgcgacga tggcgatgcg cagcgacagt agtaccgcgg
       61 tccaatcatc cggcccgaga aagtcctgca atgctatatt ctccagaata tggcaaagtt
      121 cggcttctcg accgatcgcg cacgcctgtc aagaacgacg caccttaatg cggccaatag
      181 gtcttgatga aaaaggcgca agtgatcgcg atcgtgatgc cgagcgtcac gatgcgcacc
      241 aaatgggatg gcgtaacgcg cccaagctgc gcgccgccga taccgccgat cagcgagccg
      301 gcaagcatcg cgagcgtctg cggccagagg acggcgcgcg caaacaggaa gatgatgatc
      361 gcgaccgtat tggccgctgt gatcagcagg ctgcgcggac cctgcagagc tttgatgtcg
      421 gtctcgccga agagacacca gaccgccagc atcatgacgc tggcggcgcc accgaagtaa
      481 cctccgtaaa tccctaaaat gaattgcgcc accaacacca gcggcgagcc atgaaagcgt
      541 ccttcgatgg ctattgcgat ctgtcgccca aaggcaaggg ccaaagtcgc aagcagcaaa
      601 agccaaggca gcatgattgt gaaagatgac gacggcgtgt gaagcaacaa caggctgccg
      661 atcaggccgc caactaaagt cacgataagc atcggccatt tgccgacgcc gcagatcgag
      721 cggaacgagt ttggatagat caacgtgctg gcgatcgtgc cgggccacag cgcgatcgtg
      781 ctcgacgtat ttgcgagaac agcgggcatc ccggttgcga tcatcgccgg cacggacacg
      841 aacgacccac cacccgcgag cgaattcatc gcaccagaac agaagccagc ggcaaacagg
      901 aaggcgagag cgggaaatat cacggaatga tcgaacattg tgatcagccc gtataggatt
      961 ccatggcctg gcggaatcac ttttgtccta ttgcggcgct ccgacgcgtt cgatttccca
     1021 ttcgagcgtc acgccgcttg ccgcaaagac gcggcggcga acctcttcgc cgagagattc
     1081 aatatcctgc gcgctggctg cgccgcgatt gatgaggaaa ttgcaatgca gttccgacac
     1141 ttgcgcatcg ccgacgacaa gtccgcgaca acccgcagcg tcgaccaatt gccaggcctt
     1201 atgcccggga ggatttttga acgtcgagcc gccggtgcgc gtattgaccg gctgggtttc
     1261 ttgccgtgat ttcgtgatct ccaccatctg cgcttcgatc gcggcgcgct cccccgcttt
     1321 gccttcgtaa agcgccgagg tgaaaatgag atcgtgcggc acggccgcat ggcgatacgt
     1381 gaagcccatc tctgaattgc tgaaaatgtg tagctttccg gtccgatcga tcgcgcggca
     1441 ggacacgagt acgtctttcg tctccgcgcc ataagcaccg gcattcatac gcaacgcgcc
     1501 gccgacggcg ccgggaatgc cgcgatagaa ggcaaggccc gcgatgccgg cttcagcagc
     1561 gcttgttgcg agcttcacat cgagaatccg cgcgccggcc tcgatgcgta aaccatcgat
     1621 gctcagatta tgaaagccct tgccgaggcg aaccacgatt ccggcgatgc cgccgtcgct
     1681 caccagaagg ttggagccga caccgacaac agtgacgtga atgtccgggt cgagacgctg
     1741 cagaaaatac gcaagatctt cttcatctgc cggtgtgaac agaacttgcg ctggaccacc
     1801 cgtgcgaaac caacagagat ccgccatgcg gacgttggat tcaagccggc cgcgcaaatc
     1861 cggcgcagct ttatgaagcg cgggcgtaag atcaggaaag atcatgccgc atcgccggcc
     1921 gccaattgtc ccggcagcgc ataggcccat tgcgtaatat tgccggcgcc gaggaagaca
     1981 acataatcgt ctgcttttgc gacgctacgc acgatctcgg cgagttgcgt ggggccagcg
     2041 agcggaagcg cctgacgatg gccgtgcgca cgcaacgccg cgacgagact gtcacggtca
     2101 gcgccctcaa tcgtcgcttc gccggcggca tagacgtcag cgacgatcac cacatccgcg
     2161 tcgttgaaac aggtcgcaaa ttcattgaac agggagttga gccgtgtgta gcgatgcggc
     2221 tgcacgatgg cgatcacctg cccttttgtc gacgcacgcg ccgcgcgcag caccgcggcg
     2281 atctcgaccg gatgatgccc gtaatcatcg aagatacgca cgccatgcca ttcgcccgtc
     2341 ggcgtgaaac gccgtttgac gccgccaaat ccggcaagcg cgctgcggat tgagtctgcg
     2401 ctcatgccaa gctgatacgc gacagcgatc gccgccgtcg cgttgagcgc attgtgatgg
     2461 ccgggcatcg gcagccctaa attctcaagc cgcacttcgg tgcctttgag gcgatcgtgg
     2521 atgatgacat tga
//
LOCUS       DASWOG010000403         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_155571,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000403 DASWOG010000000
VERSION     DASWOG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155571"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGB93_08380"
     CDS             <1..305
                     /locus_tag="VGB93_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0146156.1"
                     /translation="LGFPCNQFGGQEPGNEQDIGQFCTLNYGVTFPMFAKIDVNGSDA
                     HPLYKFLKEAKKGIAGTAAIKWNFTKFLVDREGNVVERFAPNVKPAEIEPEVTRLL"
     gene            374..1675
                     /gene="hisD"
                     /locus_tag="VGB93_08385"
     CDS             374..1675
                     /gene="hisD"
                     /locus_tag="VGB93_08385"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0146157.1"
                     /translation="MPQRLDTRSPDFGTAFAALLASKRESAVDVDQAVQQIIADVVAD
                     GDRALIAYSKKFDRVDLTEIGLAVSAEEIAAAELAAAPEALAALRLAHERIISFHERQ
                     RPSDVIFTDPLGVELGWRWSAIEAVGLYVPGGTASYPSSVLMNAVPAKVAGVERLVMV
                     VPAPDGLIASLVLAAAKLAGVDEIYRVGGAQAIAALAYGTQTIAPVAKIVGPGNAYVA
                     AAKRRVFGTVGIDMIAGPSEVLVIADDSANADWIAADLLAQAEHDTAAQSILITDSAA
                     LANAVAVAVERQLASLPRVAIASVSWRDHGAIIEVAAIHEALPLANRLAPEHLEIITR
                     DADALAGHVKNAGAIFIGGYTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     ILRCSPESLAALGPAAIALGKAEGLDAHARSVAFRLGLTNS"
     gene            1672..2157
                     /locus_tag="VGB93_08390"
     CDS             1672..2157
                     /locus_tag="VGB93_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEY0146158.1"
                     /translation="MSAKEPRRNRLLSVTLDETSIGRGTSDQEHERQIAIYDLVEEND
                     FGLPDRDDGPYALKISLHDAKLALEISSESGEVLAAHILSLTPFRSLLKDYFLICESY
                     YDAIRTATRAQIEAIDMGRRGLHDEAATLLQERLKGKIVCDLDTARRIFTLVTALHWK
                     G"
     gene            2256..>2524
                     /locus_tag="VGB93_08395"
     CDS             2256..>2524
                     /locus_tag="VGB93_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146159.1"
                     /translation="MAEALARKISGNKIYVASVGLRRGRIDDFAATVLREIGIEGGIR
                     APRALEDFEDEAFDLVVSLTPEARDRIAEFARGAPTTLVYWPTPDP"
BASE COUNT          468 a          786 c          788 g          482 t
ORIGIN      
        1 ttctgggctt tccgtgcaac caattcggtg gccaggaacc gggcaacgag caggacatcg
       61 gccagttctg cacgctgaat tacggcgtga ctttcccgat gttcgcgaaa atcgacgtca
      121 atggcagcga cgcccatccg ctctataaat tcctcaagga ggcgaaaaag ggcattgccg
      181 gcacggcggc gatcaagtgg aacttcacca aattcctggt cgacagggaa ggcaacgtgg
      241 tcgagcggtt cgcccccaat gtgaagccgg ccgagatcga acccgaagtg acgcgcctcc
      301 tctaggaacg cgcgcgattg ccatgcggcc ttcgcatagc taacagagaa ttcccgtcga
      361 aaggaccaga tcaatgccgc aacggctcga cacgcgcagc cccgatttcg ggacggcctt
      421 tgccgccttg ctggcgtcga agcgcgagtc ggcggtcgat gtcgatcagg ccgttcagca
      481 aatcatcgcc gatgtcgttg ccgacggcga cagggcgctc atcgcctatt cgaaaaagtt
      541 cgaccgcgtc gacctcaccg agattgggct cgcggtctct gccgaagaga ttgcggcagc
      601 cgaactggcc gctgcgcctg aggcgctggc cgcgctgcgg ctggcgcacg agcggatcat
      661 ttcgtttcac gagcgccagc gtcccagcga cgtcattttc accgatccgc ttggcgttga
      721 actcggctgg cggtggagtg caattgaggc tgttgggctt tacgtcccgg gcggaacagc
      781 cagctatccg tcctcggtac tgatgaatgc ggtgccggcg aaagtcgccg gtgtcgagcg
      841 gttggtcatg gtcgtgccgg cgccggatgg cctgatcgcg tcgctggtgc ttgccgccgc
      901 caagctcgcg ggtgtcgatg aaatctaccg cgtcggtggc gcacaggcga tcgccgcgct
      961 ggcttatggt acgcaaacca ttgcgcccgt cgcaaagatc gtcggacccg gaaacgccta
     1021 tgtcgccgcc gccaagcgcc gtgtcttcgg cacggtcggc atcgacatga tcgccgggcc
     1081 gtcggaggtg ctcgtcatcg ccgacgacag cgccaatgcg gattggatcg ccgccgatct
     1141 tttggcccag gcggagcatg acaccgcggc gcaatccata ctgattaccg acagtgcggc
     1201 gcttgcaaac gccgtagcgg tggcggtcga gcgtcagctt gcgagcctgc cacgcgttgc
     1261 gatcgcctcg gtgagttggc gcgaccacgg cgcgattatc gaggtcgccg cgatccacga
     1321 agccctgccg ctggccaatc gccttgcgcc ggagcatctt gaaatcatca cacgcgacgc
     1381 ggatgcgctc gccggtcacg tgaaaaacgc cggcgcgatc ttcatcggcg gctatacgcc
     1441 ggaagcgatc ggcgattatg tcggcggctc gaaccatgtg ttgccaaccg cgcgttcggc
     1501 gcgtttctcg tccggtctcg gcgtgctcga cttcatgaag cgtacgtcga ttttgaggtg
     1561 ttcgcccgaa agcctcgccg cccttggtcc ggcggcaatt gcgctgggca aggccgaggg
     1621 gctcgacgcc catgcccgct ctgttgcctt tcggcttggc ctgacgaact catgagcgcg
     1681 aaggagccac ggcgaaatcg tctcttgtcg gtgacgctcg acgagacttc gattggccgc
     1741 ggcacatccg atcaggagca cgaacgccag atcgcgatct acgatctcgt cgaagagaac
     1801 gacttcggcc tgccggatcg tgacgatggc ccctatgcac tgaaaatatc gctgcacgac
     1861 gccaaactcg ccctcgagat ttccagcgaa tcgggcgagg tcctcgctgc gcatattttg
     1921 tcgctgacgc cattccgcag cctgctgaaa gactattttc tcatctgcga gagctattac
     1981 gacgccatcc gcacggcgac gcgcgcgcag atcgaggcca tcgacatggg gcgacgggga
     2041 cttcacgacg aagcggcaac gttgttgcag gaacgtctca aaggcaagat tgtctgcgat
     2101 ctcgataccg cgcggcgcat cttcacgctg gtcacggctc tgcactggaa gggttaaacg
     2161 gttccgtcgt gtccccagtc gatgcaccgc aaaaacccgc gtggcgaccg caatccatcc
     2221 tcttcgtctg ttcggaaaac agcgtccgtt cgcccatggc cgaagcattg gcgcgtaaga
     2281 tttccggcaa caagatctat gttgcttcgg tcggcttgcg gcggggacgg atcgatgatt
     2341 ttgcggcgac ggtcttacgc gaaatcggga tcgaaggtgg catacgggca ccgcgcgcgc
     2401 tggaagactt cgaggacgag gctttcgatc tcgtcgtcag cctgacgccg gaagcgcgcg
     2461 atcgtatcgc ggagttcgcc cgcggcgccc cgacgacact tgtctactgg ccgactcccg
     2521 atcc
//
LOCUS       DASWOG010000404         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_9352, whole
            genome shotgun sequence.
ACCESSION   DASWOG010000404 DASWOG010000000
VERSION     DASWOG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9352"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGB93_08400"
     CDS             complement(<1..440)
                     /locus_tag="VGB93_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146160.1"
                     /translation="MSFAETILPPKEHSGWHRWGIAALIVVAIHVAILAVYLVTRPEP
                     RPPGAPPDAVMIDLAPIAVAPPPQDEVDTPPQPDQVQPYTPPPPSPVIPPPPNQPPPT
                     NDTNAPVEPDVQEAAQAEVVMSAPPPPPPPKPVVEKTEKPPKPT"
     gene            complement(437..895)
                     /gene="exbD"
                     /locus_tag="VGB93_08405"
     CDS             complement(437..895)
                     /gene="exbD"
                     /locus_tag="VGB93_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEY0146161.1"
                     /translation="MAVKLSTSDDTLEENHDINVTPFIDVMLVLLIIFMVAAPLSTVD
                     INVDLPASTAQPAQRPDKPVFVTIKPDLTVAVGDAQVSRDGLAAAVKAATGNDTQQRI
                     FLRGDKTIAYGNVVDMMNVLRKAGYLKIALVGLDASQAPDENGTTPASTP"
     gene            complement(900..1922)
                     /gene="exbB"
                     /locus_tag="VGB93_08410"
     CDS             complement(900..1922)
                     /gene="exbB"
                     /locus_tag="VGB93_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HEY0146162.1"
                     /translation="MFDARHVKAFGRVPLTVAILMALPTLGFAQAPAPSVAAPASPAT
                     AAPKPTAPAPTAAATATSAKPAVPSAPAQPIATQPAAAATAPVPAAPVSNAPVSIASQ
                     LLPQNLSAWGMFLNADIIVKIVMVGLALASLATWTVWLVKSLELANAKRNASRQLGIL
                     AHAATLDAARTDLGKGKGPVVRMVDAAAAEVDRSADLNPDGIKERAAALIERIEARAG
                     RLVNRGTGILGTIGSTAPFIGLFGTVWGIMNSFIGISRTHTTNLAVVAPGIAEALLAT
                     AFGLVAAIPAVIIYNVFARSISGYRAILGDAAAEILRHVSRDLDRGASAPTENWHVVG
                     MRRPAE"
     gene            complement(2146..3393)
                     /gene="efeB"
                     /locus_tag="VGB93_08415"
     CDS             complement(2146..3393)
                     /gene="efeB"
                     /locus_tag="VGB93_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEY0146163.1"
                     /translation="MKSSRRNFLVGAGGVLAASGIAARQPAAAEPETAAVAPETGVEP
                     FFGVHQSGIATPSQTQTYFAVFDLQTDKADDVKALLRRWTDLAARLSTGASNRATSPN
                     VLKTDSGEALGLDPNRLTLTFGFGATLFEKAGKDRYGLKARKPEALIDLPYFNGDQLV
                     EEQTGGDLSIQACANNPQVAFHAIRQLAREAHGIASIRWAQSGFSAQYGDKAPRNVMG
                     FKDGTINPQTNLDYDRFVWVGDEGPAWMHGGSYVVARLVRIAIEHWDRMDLDYQQQTI
                     GREKFSGAPLGGRKETDPLDLKAVDKDGNPLIPPTAHARIASAAMNDGAQLLRRGYSY
                     NNGATFIAERWPPWRQGIEYDAGTFFVCYQRDPRTAFVKIFDKLSKLDAMNQFVTHVG
                     SGLFACPPGIGEGGFIGDKLFET"
     gene            complement(3573..4322)
                     /locus_tag="VGB93_08420"
     CDS             complement(3573..4322)
                     /locus_tag="VGB93_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146164.1"
                     /translation="MSMLKSGYVIVGSLPSTDGTFKTAGPGCLIVFDANGKFIKTFAG
                     GRIDGPWGNSALVDHGKTATLFISQIGAGKVPSLTQAGKLASVLRLDLAIGKDGPAIT
                     KETVIGDGYDERADKDVFVIGPTGLALQDGTLYVSDAIANSVDAIPHALTRKDSAGIG
                     TPITKDGMLKRPLALDKAPNGNLLAINGLDGEIIEIDPVTKKQVAAHWINTNKAQTPP
                     GSGNLFGVALTLDGKGFYFVNDDVNTLAIAR"
BASE COUNT          787 a         1303 c         1465 g          867 t
ORIGIN      
        1 tgcgtcggct ttggcggctt ctcggttttt tcgacgaccg gcttcggcgg gggcggaggt
       61 ggcggtgccg acatcacgac ttccgcctgc gccgcctcct gcacgtcagg ttcaactggc
      121 gcgttcgtgt catttgtcgg cggcggttgg ttcggcggcg gtggaatcac cgggctcggc
      181 ggtggcggcg tgtagggctg cacctgatcg ggctgtggcg gcgtatcgac ttcatcctgc
      241 ggtggcggcg cgacggcgat cggcgcgaga tcgatcatca ccgcatccgg cggcgcgcca
      301 ggcggcctag gctcgggccg cgtcacaaga tagacggcga gaatcgccac atgaatggcg
      361 acgacgatga gcgccgcgat gccccagcga tgccagccgg aatgttcttt cggcggcagg
      421 atggtttcag cgaaactcat ggcgtcgagg ccggcgtggt cccgttttcg tccggcgcct
      481 gcgatgcatc gagaccgacc agcgcgatct tcagataacc ggccttgcgt agcacattca
      541 tcatgtcgac gacattgccg taggcgatgg tcttgtcgcc gcgcaggaag atccgctgct
      601 gcgtatcgtt gccggtcgcc gccttgacag ccgccgccaa tccatcgcgg ctgacctgcg
      661 catccccgac agcgaccgta aggtccggct tgatggtgac gaaaaccggc ttgtccggac
      721 gctgcgccgg ctgcgcggtc gaggccggaa gatcgacatt gatatcgacc gtcgagagcg
      781 gcgccgcgac catgaaaatg atgaggagga cgagcatcac gtcgatgaat ggcgtgacgt
      841 tgatgtcgtg attttcctcg agcgtgtcgt cgctcgtcga gagtttgacg gccatcggat
      901 cactccgccg ggcgacgcat gccgacgacg tgccaattct ccgttggggc ggaggcgccg
      961 cggtcgagat cgcgcgagac gtggcgcaga atttctgccg ctgcatcgcc gagtatggcg
     1021 cgatagcctg aaatgctgcg ggcgaagacg ttgtagatga tgaccgccgg aatggcggcg
     1081 acgagaccga aggcggtggc gagcagagct tcggcaatgc ccggcgcgac gacggcaaga
     1141 ttggtcgtat gcgtcctcga gatgccgatg aagctgttca tgatgcccca cacggtgccg
     1201 aagaggccga tgaagggtgc ggttgaaccg atcgtgccaa ggatgccggt gccgcggttg
     1261 acgagccggc cggcgcgcgc ctcgatgcgt tcgatcagcg ccgcggcgcg ctccttgatg
     1321 ccatcaggat tgagatccgc cgagcgatcg acctccgccg cggccgcgtc gaccatacgc
     1381 acgacggggc ctttgccctt gccgagatcg gtccgcgcgg cgtcgagcgt cgcggcatgc
     1441 gcgaggatac cgagctggcg tgacgcgttg cgcttggcgt tggcaagttc gaggctcttg
     1501 acgagccata cggtccaggt cgcgagcgag gcgagcgcca gcccgaccat tacgatcttc
     1561 acgatgatat cggcgttcag gaacatgccc caggccgaca gattttgcgg cagcaattgc
     1621 gaggcgatcg agactggcgc attcgagacg ggcgctgcgg gcaccggggc tgtcgctgcg
     1681 gccgccggct gggtcgcgat gggttgcgcg ggcgcactgg ggacagcggg ctttgccgat
     1741 gttgcggttg ctgcagccgt cggagcaggc gccgttggct tcggggctgc ggtggctgga
     1801 gaggccggcg cggcgacgct gggggcgggc gcctgcgcga aaccgagtgt cggcaacgcc
     1861 atcaatatgg cgacggtgag aggcactcgg ccaaaggcct ttacgtgacg cgcatcaaac
     1921 atgaattccg tccttgttct cagccttccg cccgtgtccg tgccaacttg cgtggcaagg
     1981 cgccaagggt caatcttgcg cggggaaata ttcgcgcgat cgatgatttc ttctatcttt
     2041 cttccaggcg cgcagccctt ctacagcggg ggctttggca agtcttcttg gtttatccaa
     2101 gaaagtttgg aagcaaactc gggaataatt cccgagcgct gccaatcacg tctcaaacag
     2161 cttgtcgcca atgaatccgc cctcgccaat gcccggcggg cacgcgaaca gaccgctgcc
     2221 gacatgcgtg acgaattggt tcatcgcgtc gagcttcgag agcttgtcga agattttgac
     2281 aaaggccgtg cgcggatcgc gctgatagca gacgaagaac gtaccggcat catattcgat
     2341 gccctggcgc caggggggcc aacgttcggc gatgaaggtc gcgccattgt tataggaata
     2401 gccacgccgc agaagctgtg cgccgtcatt catcgccgcc gaggcgatgc gtgcgtgcgc
     2461 ggtcggcggg atgagcggat tgccgtcttt atcaaccgcc ttgaggtcga gtggatcagt
     2521 ttccttgcgt ccgccgaggg gcgcgccgga gaatttctcc cgcccgattg tctgctgctg
     2581 atagtcgaga tccatgcggt cccaatgttc gatcgcgata cggacgagcc gcgcgacgac
     2641 gtagctgcca ccgtgcatcc atgccggacc ctcgtcgcca acccagacga agcggtcgta
     2701 atcgagattg gtctgcggat tgatcgtacc gtccttgaat cccatcacgt tgcggggggc
     2761 cttgtcgcca tattgggcgg aaaaaccgct ctgcgcccag cggatcgagg cgatgccgtg
     2821 cgcctcacgt gccaattggc ggatggcgtg aaacgcgact tgtggattgt tggcgcaggc
     2881 ctggatggag agatcgccgc cagtctgctc ttcgaccaat tgatcgccgt tgaaataggg
     2941 cagatcgatc agcgcctcgg gcttcctggc tttgaggccg tagcgatcct tgccggcctt
     3001 ctcgaaaagc gtagccccga agccgaaagt caacgtcagg cggtttggat cgagtcccaa
     3061 agcctcgccg ctgtcggtct tcaaaacgtt aggcgaggtt gcgcgattgg aggcaccggt
     3121 cgaaagccgt gcagcaagat cggtccagcg ccgcaacagc gccttgacgt catcggcctt
     3181 gtcggtctgc aggtcgaaaa cggcgaaata ggtctgcgtt tgcgatggtg tggcgatgcc
     3241 gctctgatgc acaccgaaga acggctcaac gcccgtctcg ggggctacgg cggccgtttc
     3301 cggctcagcc gccgctggct gacgcgcagc aattccgctg gccgccaaga cgccgcctgc
     3361 accgaccaga aaatttcgcc gcgaactctt catcgctgat tgctggacct gttctgcgag
     3421 aacctctgca ggcgcgtgcg ctctgctgca aggcggctgc ggtcacgatc ggaggcagtc
     3481 cgcctcaatc tcagatgggt ggcggtagac gattttcatc gccaccgcca ccagattctg
     3541 ctttaaaacc agtagattag aatagttcga atttaacgcg cgatggccaa ggtgttgacg
     3601 tcgtcgttga cgaaataaaa gcccttgcca tcgagcgtca gagcgacgcc gaacagattg
     3661 ccgctgccgg gtggcgtctg cgctttgttg gtattgatcc aatgcgcggc gacctgcttt
     3721 ttcgtcaccg ggtcgatctc gatgatttcg ccgtcgagcc cgttgatggc gagcagattg
     3781 ccgttcggcg ccttgtccag cgccagcggc cgcttcaaca tgccatcctt ggtgatcggc
     3841 gtgccgatgc cggcgctatc ctttcgggtt agcgcgtgcg gaatggcatc gactgaattg
     3901 gcgatcgcgt ccgagacata gagcgtgccg tcttgaaggg ccaagccggt ggggccgatg
     3961 acgaagacgt ccttgtcggc gcgttcgtcg tagccgtcgc caatcaccgt ctctttggtg
     4021 atcgccgggc cgtctttgcc gatcgcgaga tcgagacgca aaaccgacgc gagctttcca
     4081 gcctgggtca atgagggaac cttgccagcg ccgatctgcg aaatgaacag cgttgcggtt
     4141 ttcccatgat cgacgagcgc cgaattgccc cacgggccgt cgatgcgacc accggcaaag
     4201 gtcttgatga acttgccgtt ggcatcgaag acgatgagac agccggggcc agccgtcttg
     4261 aacgtgccat ccgtggaggg caagctgccg acgatcacat agcccgattt gagcatggac
     4321 attgcggtcg tcaatccgac gccgccgggg caggacggca aggtctttgg cacggaggcg
     4381 aacaaggtcg ttgtcttcct cgccgggctg taattgacga tc
//
LOCUS       DASWOG010000405         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_283974,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000405 DASWOG010000000
VERSION     DASWOG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283974"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VGB93_08425"
     CDS             complement(<1..794)
                     /locus_tag="VGB93_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146165.1"
                     /translation="MSLNSKVEATPPYGAANTETTLFTLLIMLSGAHLLNDTVQSLLP
                     AVYPILKTSFRLSFSQIGLITLAFQLTGSLLQPLIGLYTDKNPKPYSLPIGMTVTLCG
                     LLLLAFAGHYYSVLIAAALIGVGSSVFHPESSRVARLASGGQHGLAQSLFQVGGNTGQ
                     AMGPLLAALFVVPFGEKAIAWLALLPLTGIFLLSHISRWYAARLAAMPKRAKTLVGGH
                     GLTRKHVAGALAVLLALMFSKFVYLASFTSYYTFYLIQKFHVPVQTA"
     gene            complement(809..885)
                     /locus_tag="VGB93_08430"
     tRNA            complement(809..885)
                     /locus_tag="VGB93_08430"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:His,seq:gtg)
     gene            1146..>3859
                     /locus_tag="VGB93_08435"
     CDS             1146..>3859
                     /locus_tag="VGB93_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146166.1"
                     /translation="MIAYVSEPGISPQAIETALRALSLRDEAIPFFVAAPAGGAHRII
                     AASQTMLTLFEVTDLAALTARLFGGADAGAKRIGVQAQTLALDGAPRLERLNFALTPE
                     TETITFLCRRIADAGHGSLYVAASLGVGALLARRQAPVEPPAPEAEIAGPVALPPMLD
                     LDDVRKLLAARIGTRTNVRFLWRTDGTDKVTEITPPLADVVGTEAADILGKDFADVAK
                     YLDQEPLGPLARAFARRETFSGVEVLWPITGAAAAVPVGLGGLPAFDRERRFDGYRGF
                     GVIYIDRLTASTPRAFMEDAPSTPEATPDVVPDLPVEATPEPVLEPAATLPAHNVVTL
                     YPVPALKADEPQSIADELPPIAAEPPASTPEISEPLAEAREEPEEEGLLSQDEASAFR
                     AIGDVLAEDSPPPIEPQPDSEHAFSPALGSNAAAILDRLPLGILVSRDNVAIYANRTL
                     LDLLGFADEDAFHAAGGMARLFHLAPNGADVVGVRTASGETLSARARLQATEWDHLPA
                     TLLTLRRDEKADQTAQLEADLRTQKGEARELSAILDTATDGLVILDPDGRIVTLNRSG
                     EALFGYDQSEVVGQPLTVLIAADSQSKVLDYFDGMKSTGVASLLNDGREITGRAKQGG
                     LIPIFMTLSRVGPAKADPAQQKFCALFRDMTHWKKVEEELKQARQEAERTSALKSDFL
                     AKVSHEIRTPLNAIIGFAEVMLEERFGPVSNERYHDYLRDIHTSGAHVMSLVNDLLDL
                     SKIEAGKMDLQFVSVDINRAISEAVGIMQPQANQARIIMRLSLAPHLPKIVADERSLR
                     QILLNLLSNAVKFNEPGGQVIIASALTDTGNAVIRVRDTGIGMSDKDIETALEPFRQL
                     STSRQTNGTGLGLPLTKALVEANRAYFTIKSKKDEGTFVEVAFPPARV"
BASE COUNT          758 a         1195 c         1213 g          693 t
ORIGIN      
        1 gccgtctgca cgggcacatg gaacttctgg atgagataga acgtgtaata gctggtaaag
       61 ctcgcaaggt agacgaactt cgagaacatc agcgcgagca ggaccgcgag tgcgcccgca
      121 acgtgcttgc gtgtcagccc atggccaccg acgagcgtct tcgcccgctt cggcatggcg
      181 gcgagccgtg cggcatacca gcggctgatg tgagaaagca gaaaaatccc ggtgagcggt
      241 aggagcgcga gccaggcgat cgccttttcc ccgaacggca cgacgaaaag cgcggcgagc
      301 aacgggccca tggcctgacc cgtgttgcca ccgacctgaa acaaagattg cgcgagccca
      361 tgctggccgc cggacgccaa acgcgcgacg cgcgaggatt cggggtggaa caccgaggag
      421 ccgacgccga tgagcgccgc cgcgatcaga accgaataat agtgcccggc aaaggcgagc
      481 agaagcagac cgcagagcgt gaccgtcatg ccgatcggca gcgaataagg cttcggattt
      541 ttgtccgtat aaagcccgat caacggctgc aacagcgagc cggtgagctg gaaggcgagc
      601 gtaatcaagc caatctggct gaagctgagc cggaacgagg tcttaaggat ggggtagacg
      661 gctggcagca gcgactgaac ggtatcgttc aagagatgcg caccgctgag cattatcagg
      721 agtgtaaaaa gagtcgtctc ggtatttgcc gcgccgtacg gaggcgtggc ctcgaccttt
      781 gaattcaggg acatggcaag tcgcttaatg gtaccgccac cccggtttga acgggggacc
      841 tctagatcca caatctagcg ctctaaccaa ctgagctatg gcggcgtaag ggttttcgcg
      901 gcggaaacta tggcgatcgc cgcggcattg caaggcttcg cggtgcaggc cagatacgat
      961 cttaggacag ctcacaagga aatgtgtttt tgcccctccc gtacattaag catgaatcga
     1021 tcaacttggg cagaaatgct agggattaat agaccgccgc ccgacggggc attaggacct
     1081 tgttaaggct atccgtggcg gcggaggggg gccgaaggat acgacggaag agagcctctg
     1141 ccgcgatgat tgcctatgtc agcgagcccg ggatcagccc ccaggcgatt gagactgcac
     1201 tccgggcttt atccctccgt gacgaggcga tcccgttttt cgtcgccgcg cctgcgggcg
     1261 gcgcccaccg aatcattgct gccagccaga cgatgctgac gctgttcgag gttacggacc
     1321 ttgcggcgct caccgcgcgg ctctttggcg gcgcggacgc tggcgccaag cgaatcggcg
     1381 tccaggcgca gaccttggcg ctcgacggcg cgccgcggct tgagcgtttg aatttcgcgc
     1441 tgacgccgga aaccgagacg atcaccttcc tgtgccgccg tattgcggac gccgggcacg
     1501 gctcgctcta tgtcgccgcc tctctcggtg tcggcgcgct tctggcgcgg cgtcaggcgc
     1561 ccgtggaacc accggcgccg gaagctgaga tcgctggccc tgtcgccttg ccgcctatgc
     1621 tggatctcga cgatgtgcgc aagctgctcg cggcccgcat cggcacgcgc accaatgtcc
     1681 gttttctctg gcgcaccgac ggcaccgata aggtcaccga gatcacgccg ccgctagccg
     1741 acgtggtcgg caccgaggcc gccgatatcc tcggcaagga tttcgccgac gtcgccaaat
     1801 atctcgatca ggaaccgctt gggccgttgg cgcgcgcctt cgcccggcgc gaaaccttca
     1861 gcggcgtcga agttttgtgg ccgatcacgg gtgccgccgc cgctgtgccc gtcgggcttg
     1921 gcggtcttcc ggcgttcgac cgcgaacggc ggttcgacgg ttatcgcggc ttcggcgtca
     1981 tctatatcga tcggctgact gcaagcacgc cgcgggcctt catggaagat gcaccgtcga
     2041 cgcccgaggc gactccggac gttgtgccgg atttgcctgt tgaagcgaca cccgagccgg
     2101 tgctggaacc cgcagcgacg cttccagcgc ataatgtcgt gacgctttat ccggtcccgg
     2161 cgctaaaagc ggatgagccg cagtcgatcg cagacgaact gccaccgatt gcggcggagc
     2221 cgccggcaag cacgccagag atttcagagc ctttggctga agcgcgcgag gaaccagaag
     2281 aagagggctt gctctcgcag gacgaggcct cggccttccg agccatcggc gacgtgcttg
     2341 ccgaagattc tccgccgccg atcgagcccc aacccgattc tgaacatgcg ttttcgccgg
     2401 cgcttggcag caatgctgcg gcgattctcg accgtctgcc gctcggcatt ctggtcagcc
     2461 gcgacaatgt cgcgatttac gccaaccgca cgctgctcga tctgctgggt tttgccgacg
     2521 aggatgcgtt tcatgcggcg ggcggtatgg cacgtctgtt tcacctcgcg cccaacggcg
     2581 ccgatgtggt tggtgtgcgt accgcttccg gtgaaacgct ctcggcgcgg gcgcgcctgc
     2641 aggcgaccga atgggatcac ctgccggcca ctttgctgac cttgcgacgc gatgagaaag
     2701 ccgaccagac ggcgcagctc gaggccgatc tgcgcaccca aaaaggggag gcgcgcgagc
     2761 ttagcgcgat cctcgatacg gcgaccgacg gcctggtgat tctcgatccg gacggccgga
     2821 tcgtgaccct caaccggtcc ggcgaagcgc ttttcggcta cgaccagagc gaggtcgtcg
     2881 gccagccgct caccgtcctc atcgccgccg acagccagag caaagtgctc gactatttcg
     2941 acggcatgaa atcgaccggc gtcgccagtc ttctcaacga tggccgcgag atcaccggcc
     3001 gcgccaaaca gggtggcctc atcccgatct tcatgacttt gagccgcgtc gggccggcga
     3061 aggccgatcc ggcgcagcag aaattctgcg cccttttcag ggatatgacg cattggaaga
     3121 aagttgagga agaacttaag caggcgcgtc aggaggccga acgcacgagc gcattgaagt
     3181 ccgattttct cgccaaagtc agccacgaaa tccgcacgcc gctcaatgcg atcatcggct
     3241 ttgccgaagt gatgctggag gagcgtttcg gccccgtgag caacgaacgc taccacgatt
     3301 atctgcgcga catccataca tccggcgcgc atgtgatgag cctcgtcaac gatctgcttg
     3361 atctttcgaa gatcgaagca ggcaagatgg acttgcagtt cgtctcggtg gacatcaacc
     3421 gggcgatctc ggaggctgtg ggcatcatgc agccgcaggc caatcaggcg cgcatcatca
     3481 tgcgtctgtc gctggcgccg catctaccca agatcgttgc cgacgaacgc tcgttgcggc
     3541 aaatcctgct caaccttttg tcgaacgcgg tgaagttcaa cgaacccggc ggccaggtga
     3601 tcatcgcctc ggccctgact gacaccggca atgccgtgat ccgcgtccgc gacaccggca
     3661 tcgggatgtc cgataaagac attgagacgg cgcttgaacc cttccgccag ttatcgacct
     3721 cgcgtcagac gaatgggacc ggcctcggtc tgccgttgac caaggcgctc gtcgaagcca
     3781 accgcgccta tttcacgatc aagagcaaga aggatgaggg caccttcgtc gaggtcgcct
     3841 ttccgccggc gcgcgtttt
//
LOCUS       DASWOG010000406         5039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_266333,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000406 DASWOG010000000
VERSION     DASWOG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266333"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="pstS"
                     /locus_tag="VGB93_08440"
     CDS             complement(<1..926)
                     /gene="pstS"
                     /locus_tag="VGB93_08440"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0146167.1"
                     /translation="MSFKTLAKVGLIAGAVIGAVVAAEATDISGAGATFPYPIYSKWA
                     AAYKTETGDSLNYQSIGSGGGIKQIEARLVTFGATDKPLEVADLDKNGLVQWPQVMGG
                     IVPVINLDGIAAGSIVLDGPTLAKIFLGEIKTWDDPAIAKLNADVKLPSTAIAVVHRA
                     DGSGTTFNFTDYLSKVSPDWKAKVGSATAVQWPAGIGAKGNEGVANNVIQTKGSIGYV
                     EYAYALQNKLTYTKLINGAGKVVAPTSAAFAAAAANADWAHAPGFHLILTNQPGDASW
                     PLTAATFILLPKQPKDEAAAKAALKFFAWAFD"
     gene            complement(1066..2124)
                     /locus_tag="VGB93_08445"
     CDS             complement(1066..2124)
                     /locus_tag="VGB93_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146168.1"
                     /translation="MDEKTRNRDRSLIRNFAEAMPQAVIVIDRHDRIVAANSAAHAIL
                     PGLQTDIPLARTLRSPDVLDAIDRVRDAGRSQRVVWVEKFPVERLFEVNIAPLADDNF
                     EQALFLSLHDLSDARRLERMRVDFVANASHELRTPLASLLGFVETLEGPAKEDAEART
                     RFLAIMREQAQRMSRLVDDLLSLSRIEQHLHVRPETPVDLVGIFKHIADTLSPVAEER
                     DVELVIEAPAEVVVPGDRDELLRVAENLIENAIKYGASDPATKDRRVVIELGEQGRQA
                     HIAVRDFGTGIAPEHLPRLTERFYRVDAGQSRAKGGTGLGLAIVKHIIARHGGRLNIE
                     SKWKEGATFIVTLPLFES"
     gene            complement(2154..2735)
                     /locus_tag="VGB93_08450"
     CDS             complement(2154..2735)
                     /locus_tag="VGB93_08450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0146169.1"
                     /translation="MVTLSKIYTRGGDAGLTSLGDGERRRKDDLRIEAYGTVDEANSF
                     LGVARAALSEASNEAKLSQILAAIQNDLFDLGADLCVPEGAGAPSRLRVTQQQVDRLE
                     HEIDALNADLLPLTSFVLPGGSQAAAAMHVARAIVRRAERVMVTLTAQPGESVGAPAL
                     KYINRLSDFLFVAARHLNRFGEGDVLWTPGGVP"
     gene            complement(2740..2928)
                     /locus_tag="VGB93_08455"
     CDS             complement(2740..2928)
                     /locus_tag="VGB93_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY0146170.1"
                     /translation="MHQLGNVLVAVAVFAVFLVLVAGLVNMMRGGSPNLSQKLMRWRV
                     GLQALAILIIIGVLFLRR"
     gene            complement(2982..>5039)
                     /locus_tag="VGB93_08460"
     CDS             complement(2982..>5039)
                     /locus_tag="VGB93_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEY0146171.1"
                     /translation="EASYRIDAWLDPPAYTGKPPVLLKHGQPDQATRVEAPIDSVLII
                     RGAGGDVGVETQGGLEAAKKEHDAPQAPGESERRLILRGDAQLTLKHAGTTVGTFAIS
                     VIPDNPPEIELTDAPRFNARGSLTLAYKISDDYGAISAEAVFADPHVDGVAGTVRSVV
                     GPPRLSLVLPPGGTGEAETTGDLSDHPWSGARVTMALVAHDEGGNIGRSDPIEITLPQ
                     RPFVKPIAKALVEQRRNLVLYPEARDKVETALDGLMIAPDVFGVPASVFLGLSVAADM
                     VQDAQSDKDIVDTADFLWAMALQIENGSLADAERDLRSAEQKLRDALQRNASQDEIRK
                     LTDNLRAAMDKFLREFAARQAQQRQQDGARSPNAPQSVSRQDLQSMIDRMMQMANAGD
                     KEDAQKMLEQLQNTLENLQQAGQQNQDPTTQAMNQAMRDLDRLTHDQQKLRDQTQRLA
                     HSQDSDQPDQSGQQQPMTAQQLHDQQQALRQALQKLQNELKKFGQNQSGLKDAQKAMQ
                     DAEKSLSPGRQAGQDQQGQSQDNQGQQPQGQQGQGQQGQQGQQQGGQQGQGDQGQGDQ
                     QGQGQSPGDSAVASQGRALEGLRKGADQLAQSMQKGQGQGQRPGSQSGEGQAEGAGDQ
                     DPLGRPMANDPVLNPDSRLNMEGLPAAERAQRVLEELRRRLSDPWRSQEELDYLERLL
                     KPY"
BASE COUNT          884 a         1590 c         1556 g         1009 t
ORIGIN      
        1 ttgtcaaagg cccaagcgaa gaatttcagc gcggctttgg cggccgcctc gtccttcggc
       61 tgcttcggca gcaggatgaa ggtcgcggcg gtgagcggcc acgaggcatc gcccggctgg
      121 ttggtgagga tcaggtggaa gcccggggcg tgcgcccagt cggcgttcgc cgcagcggcc
      181 gcgaaggcgg ccgaggtcgg cgccacaacc ttgccagcgc cgttgatgag cttggtgtag
      241 gtcagcttgt tctgcagggc ataggcgtat tcgacatagc cgatggagcc cttggtctgg
      301 atgacgttgt tggcgacacc ctcgttaccc ttggcgccga tgccggccgg ccattggacg
      361 gcggtcgccg agccgacctt cgccttccaa tccggcgaaa ccttcgacag atagtcggtg
      421 aagttgaagg tggtgcccga gccgtcggcg cggtggacaa ccgcaatcgc ggtcgaaggc
      481 agcttgacgt ccgcgttcag cttggcgatt gccgggtcgt cccaggtctt gatctcgccc
      541 aggaaaatct tggcgagggt cgggccgtcg agaacgatcg aaccggcggc aatgccgtca
      601 agattgatga ccggaacgat gccgcccatg acctgcggcc attgaacgag accgttcttg
      661 tcgaggtcgg caacttcgag cggcttgtcg gtcgcgccga aggtcacgag gcgcgcttca
      721 atctgcttga tgccgccgcc ggagccgatc gactgatagt tcaggctatc gccggtttcg
      781 gtcttatagg ccgcagccca tttggaatag atcggatagg ggaatgtcgc cccggcacct
      841 gagatgtcgg tagcctcggc ggcgacaacg gcgccgataa cggcaccagc aatcaaaccc
      901 accttggcga gggtcttgaa actcatctta agcctctcaa aactgagaaa atagcgctgt
      961 acgtccggcc ttcggcccaa ccgcgcggcc gcaccctagg aaggtcgggc aacgcattta
     1021 cgacggtcat atgacgttta catgacgggg gtcgcgacgt gccttttagc tttcgaaaag
     1081 cggcagcgtc acaatgaagg tcgcgccttc tttccatttg gattcaatgt tgaggcggcc
     1141 gccatggcgc gcgataatat gtttgacgat cgcgaggcca agccccgtgc cgcccttcgc
     1201 gcggctctgc ccggcatcga cgcgataaaa gcgctccgtc aggcgtggta aatgttccgg
     1261 cgcgatgcct gtgccgaaat cacgcacggc gatatgcgcc tggcgtccct gctcgccgag
     1321 ttcgatgacg acgcgccggt ccttcgttgc cgggtcggac gcgccatatt taatcgcatt
     1381 ttcgatcagg ttttccgcca cgcgcagaag ctcatcgcga tcacccggca cgacgacttc
     1441 ggccggcgct tcgatgacga gttcgacgtc gcgctcttcc gcgacaggcg acagcgtatc
     1501 ggcgatatgt ttgaaaatgc cgacgagatc gacaggcgtt tccggccgca catgcaggtg
     1561 ctgctcgatg cgcgacaacg acagcaggtc gtcgacgaga cgcgacatgc gctgcgcctg
     1621 ctcgcgcatg atcgccagaa agcgggtgcg cgcttccgca tcttcctttg ccgggccctc
     1681 cagcgtttcg acgaagccga gcagcgacgc gagcggcgtg cgcaattcgt ggctggcgtt
     1741 ggcgacgaaa tcgacgcgca tacgctcgag gcggcgggca tcgctgagat catgcaacga
     1801 tagaaacagc gcctgctcga aattgtcgtc ggcaagcggt gcgatattga cctcgaacag
     1861 gcgctcgacc ggaaactttt cgacccagac gacacgctgg ctgcggcccg catcgcgcac
     1921 acggtcgatg gcgtcgagca catcgggcga gcggagcgtc cgcgccagcg gaatatcggt
     1981 ttgcagaccc ggcaaaatgg cgtgcgcagc ggaattcgcc gcaacaatac ggtcatgcct
     2041 gtcgatgaca atgacagcct gcggcatcgc ctccgcaaaa ttgcggatga gggaacggtc
     2101 gcgattgcgg gtcttctcgt ccataccgcc ctgaaacagg ttgtcgtcaa aattcaaggc
     2161 acgccgcccg gcgtccaaag aacatcgcct tcgccgaagc gattgagatg ccgcgcggcg
     2221 acgaagagga aatccgataa tcgattaatg tatttcaagg ccggtgcgcc gacgctttcg
     2281 ccgggctgcg ccgtcagcgt caccatcacc cgctctgccc gccgcacaat tgcgcgtgcg
     2341 acatgcatcg ccgccgcggc ctgcgaaccg ccgggcagca cgaaagacgt gagcggcagg
     2401 agatcggcgt tcagggcatc gatttcgtgt tcgaggcgat cgacttgctg ttgcgtcacc
     2461 cgcagccgcg acggcgcacc cgccccttcc ggcacgcaaa gatcggcgcc gagatcgaaa
     2521 agatcgttct ggatcgcggc aagaatctgg gagagtttag cctcgttcga ggcttcgctc
     2581 aatgcggcgc gcgcgacgcc gaggaaagaa ttcgcttcat cgaccgtacc ataggcctcg
     2641 atccgcagat cgtccttgcg ccggcgctcg ccgtcgccaa gcgacgtcag gcccgcgtca
     2701 ccgccgcgtg tataaatttt gctgagcgtg accaccggcc tacctccgca aaaacagaac
     2761 gccgatgatg atgagaatgg cgagtgcctg caatccgacg cgccaacgca tcaacttttg
     2821 cgacaggttc ggactgccgc cgcgcatcat gttcaccaag ccagcgacga gcaccagaaa
     2881 gacggcaaaa accgccacgg ccacaagcac attgcccaac tgatgcatgg tcgtttcttt
     2941 agcgcgaagt caaacgcgtg tcacaaactt tgtttgcgcg ctcaataggg cttcagcagc
     3001 cgttccagat aatcgagctc ctcttgcgac cgccaggggt cgctcaagcg gcggcgcaat
     3061 tcctcaagca cgcgctgcgc ccgctcggcc gccggcaggc cttccatatt cagccgcgag
     3121 tctggattga gcaccggatc gttagccatc ggccggccaa gtggatcctg atcgcccgcg
     3181 ccttcggcct ggccttcgcc gctctggctg ccgggccgct gcccctggcc ctgtcccttc
     3241 tgcatcgact gcgcgagttg atcggcgcct ttgcggagtc cttcgagggc gcggccctgc
     3301 gaggcgacgg cggaatcgcc ggggctttga ccctggcctt gctggtcgcc ttgaccctgg
     3361 tcgccctgac cttgttgtcc gccctgctgc tggccttgtt gaccctgttg tccttggcct
     3421 tgctgccctt gcggctgctg accttgattg tcctggctct ggccctgctg atcctgtccc
     3481 gcctggcgcc cggggctcag cgatttctcg gcatcctgca tggccttctg tgcgtctttc
     3541 aggccggatt ggttctgccc gaatttcttg agctcgtttt gcagcttctg cagcgcctgc
     3601 cgcagcgcct gctgctgatc gtgcaattgc tgcgcggtca tcggctgctg ctggccgctc
     3661 tggtccggct gatccgaatc ctgcgaatgc gcgagccgct gcgtctggtc gcgcaacttc
     3721 tgctggtcat gtgtcaggcg gtcgagatcg cgcatcgcct gattcatcgc ctgcgtcgtc
     3781 gggtcctgat tctgctgtcc ggcctgctgc agattttcga gcgtgttctg caattgctcg
     3841 agcattttct gcgcgtcttc tttatcgccg gcattggcca tctgcatcat gcggtcgatc
     3901 atggattgta aatcttgccg gctgaccgat tgcggggcgt tgggcgagcg cgcgccgtct
     3961 tgctgacgtt gctgcgcctg gcgggcggcg aattcgcgca agaacttgtc cattgccgcg
     4021 cgcaaattgt cggtcagctt gcgaatttca tcctgcgaag cattgcgttg caaagcgtcg
     4081 cgcaatttct gctcggccga acgcaaatcc cgctccgcat ctgcgaggct gccgttctcg
     4141 atctgtagcg ccattgccca gagaaaatcc gccgtatcca cgatgtcctt atcgctctgc
     4201 gcgtcctgca ccatgtctgc cgcgaccgag aggccgagga aaaccgatgc cgggacgccg
     4261 aacacatccg gcgcaatcat caaaccatcg agcgccgtct ccaccttgtc gcgtgcttcg
     4321 ggatagagaa cgagattgcg ccgctgctcg acaagcgcct tggcgatcgg cttgacgaac
     4381 gggcgttgcg gcaaggtgat ttcaatcgga tcgctgcggc cgatattgcc gccttcatca
     4441 tgcgcgacga gcgccatcgt cacacgcgcg ccggaccacg gatgatcgga aagatcgccg
     4501 gtcgtctcgg cctcgccagt gcctcccggc ggcaggacca aggagaggcg cggcggtcca
     4561 acgaccgaac ggaccgtccc ggcgacgcca tcgacatgcg gatcggcgaa caccgcttcg
     4621 gcggaaatcg ccccgtaatc gtcgctgatc ttgtaggcga gtgtaaggga accccgcgcg
     4681 ttgaagcgcg gcgcatcggt caattcgatt tccgggggat tgtccggaat gaccgagata
     4741 gcgaaagttc cgacggtcgt gccggcatgt ttcaatgtga gctgtgcatc gccgcgtagt
     4801 attaaacgac gctcgctttc gcccggcgct tgcggcgcgt cgtgttcttt tttcgcggcc
     4861 tcaagaccgc cctgggtttc gacgccgacg tccccccccg caccgcgtat aatcagcacc
     4921 gaatcgatcg gcgcttcgac tctcgtcgct tgatcgggct ggccgtgttt cagcagcacg
     4981 ggcggtttgc cggtgtaggc gggcggatcg agccaggcgt cgatccgata gctcgcttc
//
LOCUS       DASWOG010000407         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_64156,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000407 DASWOG010000000
VERSION     DASWOG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64156"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..404
                     /gene="rplV"
                     /locus_tag="VGB93_08465"
     CDS             12..404
                     /gene="rplV"
                     /locus_tag="VGB93_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY0146172.1"
                     /translation="MGKQQAPRALKDNEAKAVCRMIRVSPQKLNLVAQLIRGKKVERA
                     LAELEFSRKRIAVDVKKALESAIANAENNHNLDVDDLVVAEAFVGKGLVLKRFSPRAR
                     GRAGRIEKPFSNLTIVVREVEAPAPAGA"
     gene            415..1140
                     /gene="rpsC"
                     /locus_tag="VGB93_08470"
     CDS             415..1140
                     /gene="rpsC"
                     /locus_tag="VGB93_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY0146173.1"
                     /translation="MGQKVNPIGLRLGINRTWDSRWYANKGEYSKLLHEDIGIRELLF
                     KMLKQAAVSRIIIERPHKKCRVTIYSARPGVVIGKKGADIDKIRKAVAKLTESEVVIN
                     IVEVRKPEVDATLIADSIAQQLERRVAFRRAMKRAVQSAMRLGAEGIRINCSGRLGGA
                     EIARLEWYREGRVPLHTLRADIDYGTATAHTAYGTCGIKVWVFKGEILEHDPMAQDKK
                     MAEQDHGGGGGGERRPRRDRDAA"
     gene            1174..1587
                     /gene="rplP"
                     /locus_tag="VGB93_08475"
     CDS             1174..1587
                     /gene="rplP"
                     /locus_tag="VGB93_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY0146174.1"
                     /translation="MLQPKRTKFRKAFKGRIHGDSKAGYNLDFGEYGLKAMEPDRVTA
                     RQIEAARRAMTRHMKRAGRVWIRIFPDVPVSKKPTEVRMGKGKGAPEFWAVRIAPGRI
                     MFEIGGVPTELAREALTLAAAKLPIKTRFVQRIVE"
     gene            1598..>1788
                     /gene="rpmC"
                     /locus_tag="VGB93_08480"
     CDS             1598..>1788
                     /gene="rpmC"
                     /locus_tag="VGB93_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0146175.1"
                     /translation="MKNKQRLSDLRVMSADQLEHEVLNLKKEQFNLRFQRATGQLQDT
                     ARVRIVRRDIARAKTIAAT"
BASE COUNT          380 a          514 c          587 g          307 t
ORIGIN      
        1 tgaggcgcat tatgggcaag cagcaagcgc cgcgcgcttt gaaagacaat gaggcgaaag
       61 ccgtctgccg gatgatccgt gtcagcccgc agaagctgaa cctggtcgcg caattgatcc
      121 gcggcaagaa ggtggaacgc gctttggccg agctcgaatt ttcgcgcaag cgcatcgcgg
      181 tcgacgtcaa gaaggcgctc gaaagcgcga tcgctaacgc tgaaaacaat cataacctcg
      241 atgtcgacga tctggtcgtc gccgaggcgt tcgtcggcaa gggcctggtg ctcaagcgtt
      301 tttcgccgcg tgcgcgcggt cgcgccgggc ggatcgagaa gccgttttcg aatttgacca
      361 tcgtcgtgcg cgaggtcgaa gcgccggcac cggccggggc ttgaggagca agcaatggga
      421 cagaaagtca atccaatcgg cctgcgcctc ggcatcaacc ggacctggga ttcgcgctgg
      481 tacgccaaca agggcgaata cagcaagctg ctgcacgagg acatcggcat ccgcgagctt
      541 ctgttcaaga tgctcaagca ggcggcggtg tcgaggatca tcatcgagcg gcctcataag
      601 aagtgccgtg tgacgatcta ttcggcgcgt cccggcgtgg tcatcggcaa gaagggcgcg
      661 gacatcgaca agatccgcaa ggccgtggcg aagctcaccg aatccgaggt cgtcatcaat
      721 atcgtcgagg tgcgcaagcc cgaggtcgac gcgacattga tcgccgactc gatcgcccag
      781 cagctcgagc gtcgcgtcgc tttccgccgc gcgatgaagc gagccgtgca atcggcgatg
      841 cgtcttggcg ctgagggcat tcgtatcaat tgctccggtc gtcttggcgg cgccgaaatc
      901 gcccgtctcg aatggtatcg cgaaggccgt gtgccgctgc atacgctgcg cgccgatatc
      961 gattacggca ccgcgacggc gcatacggct tatggcacgt gcggcatcaa ggtctgggtg
     1021 ttcaagggcg aaatccttga gcacgatccg atggcgcagg acaagaagat ggccgagcag
     1081 gatcatggcg gtggtggtgg cggtgaacgt cgcccacgcc gcgaccgcga cgcggcctga
     1141 gaacgacgcg aaagattttg aaagactaaa accatgttgc aacctaagcg cacgaagttc
     1201 cgcaaagctt tcaagggccg tattcacggc gactcgaagg ctgggtacaa tctcgacttc
     1261 ggcgaatatg ggctgaaggc gatggaaccc gatcgcgtca ccgcacgcca gatcgaggcg
     1321 gcgcgacgcg ccatgacgcg tcacatgaag cgcgccggtc gcgtctggat ccgaattttc
     1381 ccggacgtgc ctgtgtcgaa gaagccgacc gaagtccgca tgggtaaggg taagggcgcg
     1441 ccggaattct gggccgtgcg tatcgcgcct ggccgcatca tgttcgagat cggcggtgtg
     1501 ccgacggaac tcgcgcgtga agccttgacg ctggcagccg ccaagctgcc gatcaagaca
     1561 cgcttcgtac aacgtatcgt ggaataggag gcgcgccgtg aagaacaagc aacgtttgtc
     1621 ggacctgcgc gtcatgtccg cggatcaatt ggaacatgaa gtcctgaacc tcaagaagga
     1681 gcagttcaat ctgcgctttc agcgggcgac cgggcagctt caggatacgg cgcgggtgcg
     1741 catcgtccgc cgcgatatcg cgcgggcgaa gacgatcgcc gccacgca
//
LOCUS       DASWOG010000408         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_45507,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000408 DASWOG010000000
VERSION     DASWOG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45507"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..219
                     /locus_tag="VGB93_08485"
     tRNA            143..219
                     /locus_tag="VGB93_08485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:177..179,aa:Arg,seq:ccg)
     gene            628..1701
                     /locus_tag="VGB93_08490"
     CDS             628..1701
                     /locus_tag="VGB93_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0146176.1"
                     /translation="MFERDAHALDRPQGGTLDLHEESGLFALKRAGLTEVFLKVARYE
                     DQGERLLDQHGHVHFEYEEAGAGDRPEVDRTALRDLLLDALPPACVRWASPVREVLPV
                     ETGWNVVLDDRSEGPFDLVVGADGTWSRVRPLLSSYRPQYSGLTFIEFGIDDIDRRFP
                     ALSKLVGRGKVGVIGEGREIIAQRNGNAHVRGYAIFRVPADWAERRFDFSSPGLVRTE
                     LLREFRDFADEITGLFCAANDHFIARQIYALPVGHRWTHRPGLTLIGDAAHVMAPFGG
                     EGVNAALLDAARLAERLIQGTDLSAAVLAYEEEMFARVEEAAGESSDAAATFLSHDSL
                     AETLAMYRDRRARMQAAVCAGAY"
     gene            complement(1715..1873)
                     /locus_tag="VGB93_08495"
                     /pseudo
     CDS             complement(1715..1873)
                     /locus_tag="VGB93_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          345 a          560 c          578 g          390 t
ORIGIN      
        1 cttgccggta ctgaaagtat tttggctctg gtgaggcgcg acgcctcacc ctggcaattg
       61 gtgatatttc gttctaacgc ccgagacagc gaggcatggt gtttgcggct ccccgtcgaa
      121 tgagctatca gtctcgcccg aagcacccgt agctcagctg gatagagtgt cgccctccga
      181 aggcgaaggt cgcaggttcg aatcctgccg ggtgcgccat ctgcgaacaa aactgcgaac
      241 tccaggcaca gggccgccgt ccgccaggac ggcttgctct agcacgtctt tccggccgac
      301 gatgcggatt tcgcgatcgt ccacttcgac gcggtcaatg agggcaccga gataagcctt
      361 gcggaacggc acctcgcctg aggtcagctt ctcgcgcatc gttgtgccga aacgctcgat
      421 gacgatcggg ctgatatcta cggctggcct tgcgccggat ttcgcccgct ccaaggccgc
      481 cagcgcggta tcccgctcga ctttcagggc ggtgacacgg tctttgagga tatcgtccat
      541 ctcggcaacg ccgtcctcga cggccttgta aagccggcgc aggcgctcgg tggcctcatc
      601 cttcacgtcc atagcatcgc cgctactgtg ttcgaacgcg atgcccatgc ccttgatcga
      661 ccgcagggcg gcacactcga tctccatgag gagtcgggac tgtttgcgtt gaagcgagca
      721 ggcctcaccg aggtatttct caaggtggcg cgctacgagg atcagggcga gcggctcctc
      781 gaccagcacg gccacgtgca ctttgaatac gaggaagccg gcgccggcga tcgtccggaa
      841 gtcgatcgaa cggcactgcg tgacttactg ctcgatgcgt tgccgcccgc gtgcgtgaga
      901 tgggctagcc ccgtgcgtga agtattgccc gtggaaacgg ggtggaatgt tgtcctcgac
      961 gaccggagcg aaggaccgtt cgacctcgtc gtcggcgcgg acggcacctg gtctcgggta
     1021 cggccgttgc tgtcttccta caggccgcaa tatagcggcc tgacgtttat tgaatttggt
     1081 atcgacgata tcgatcgccg ttttcctgca ctctccaaac tcgtcgggcg cggcaaggtc
     1141 ggagttatcg gcgaaggccg cgagatcatc gctcagcgca acggcaatgc gcacgtacga
     1201 ggctatgcga tcttccgtgt cccggccgac tgggcagaac ggcgctttga tttttcctcg
     1261 ccgggattgg ttcgcacaga gcttctgcga gagttcagag actttgcgga tgagatcacc
     1321 ggtctcttct gcgccgcaaa cgatcatttc atcgctcgac aaatctatgc gctaccggtc
     1381 ggtcatcgtt ggacacaccg tcctggcctg acactgatcg gcgacgccgc gcatgtgatg
     1441 gcgccatttg gtggcgaagg ggtcaacgcc gcgttgctcg atgccgcccg cctggctgaa
     1501 cggctgatcc aagggacaga tctgtccgct gcagtgcttg cctacgagga ggagatgttc
     1561 gcgcgtgtcg aagaggcggc aggagaatcg tcggatgccg cggcaacatt cctttcgcac
     1621 gacagcctcg cagaaacgct cgcaatgtat cgggatcggc gggcgcgcat gcaggcagca
     1681 gtatgtgccg gtgcttattg atcggccagc agcacttatg aagataagcg cagaagatat
     1741 cggccatcgc atcggcgtaa gcttcgatct ccgcagatgt tcgaggtgtc tccgagaact
     1801 ccttgccaac cgacgacggc gtctttttga tcagatcgcc ggccaaaaca cgtctgtttc
     1861 ggggcctcgg gca
//
LOCUS       DASWOG010000409         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_137845,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000409 DASWOG010000000
VERSION     DASWOG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137845"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..255)
                     /locus_tag="VGB93_08500"
     CDS             complement(28..255)
                     /locus_tag="VGB93_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY0146177.1"
                     /translation="MIAEANLFGVYFSGALVEALAAFIALMIIRPLLRWLGFYQIVWH
                     RRLVDIGLFVILWGVMVETLPPLGGAGTWLR"
     gene            complement(252..2285)
                     /locus_tag="VGB93_08505"
     CDS             complement(252..2285)
                     /locus_tag="VGB93_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0146178.1"
                     /translation="MGIFRARPFIFAINAYLATALALYIALALDLPNPWWAMVTVFLA
                     QPTQLLVGAIWAKAAYRIVGTLIGAAASLLIIPNLSQAPELMILALAGWIGLCLYGAL
                     LDRTPRAYVFMLAAYTVALVGLPQATDPTALFNISVARAEETVIGVLASAVVQSILFP
                     RSVTTFMQGKLNEILADARTSIGDVLAEPALLGPALAHQHIATGLTDLSLMATNLRFE
                     EDFPRSARRVLRALEERLVSLLPLTSAVEDRLKALRETGPIPPIVEEIVSAVVAWLKG
                     AQSADKAGYARIMDEIARLHPRTDAAAEWPDLLCASLSARLADLARAWQESLVLTSAL
                     GNPTRLDPEVQDLCGERRPRTLHTDAGLALFSAIVVALTIIGISTFTIATKWESGATA
                     IAITSVLCSIFIAADDPTPLAGTLIYGFFLAFPVTVFYEFAILQSIDGFVMLALVLFP
                     VVFLAGFFFAQPKYATMALGSMVGFSAGLALQPEFLSNFAAFMNAYIALLVGAAFGYV
                     CLSTFRVLPARRVAQRIRRAGWSDLAALTASPSNEVAWSSLMIDRMSLLISRLARLPA
                     GSDVELMDALTDLRLGVTIIELSQVCAAVDRPEKERIESFMAMLGRHFKSLASGRRAS
                     LPQNAVGTLDAIMAHIIQLPKTAERTTGLLAAVGLRRGLFPQATAYQPMVSAK"
     gene            complement(2384..2908)
                     /locus_tag="VGB93_08510"
     CDS             complement(2384..2908)
                     /locus_tag="VGB93_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146179.1"
                     /translation="MQSLVRIFIGSLGVAATLVSTGAKAATDPLDTASLTELRQLYKQ
                     LIDAENAHDVRAVAPYVWDSPSALFVAKTSDPSQGNWAGFWGKGVVLAHFSALYKGTF
                     HMAPDYARERIVGLTKDVAETYIPLQISVSYAGQSPVPKPFLMIVEWIRTPQGWRMAT
                     DIALPVPPAPPKHT"
BASE COUNT          738 a          931 c          924 g          571 t
ORIGIN      
        1 tggtgcgcag caccgaagca atattactca acgcagccac gtacctgctc ctcccaaggg
       61 cggaagcgtc tccaccataa cgccccaaag gattacaaac agaccgatgt cgaccaatct
      121 gcggtgccat acaatctggt agaaaccaag ccagcgtaat aacggcctta taatcatcag
      181 agcaatgaag gcggccaaag cctcaaccaa tgcgccgctg aaataaacgc caaaaagatt
      241 agcttctgcg atcacttagc gcttaccatg ggttgataag cggtggcctg tggaaaaagg
      301 ccacgccgaa gacccacagc ggcgagaagc ccggtcgtcc gctccgccgt cttgggaagc
      361 tgaatgatgt gcgccatgat ggcgtcaagc gtgccgacgg cattttgtgg caaagacgcg
      421 cggcgcccgc ttgccaacga tttgaaatgt cggccgagca ttgccatgaa gctttcaatg
      481 cgttcctttt cgggtcgatc gaccgcagcg cacacctggc tgagttcgat gatggtgacg
      541 cctaaacgca agtcggtcaa agcatccatt aattcaacgt ctgaaccggc tggcagcctg
      601 gcaagacgcg aaatgaggag actcattctg tcgatcatca agctcgacca cgcaacttcg
      661 ttggatggcg acgctgtcag cgctgcgaga tcgctccagc cggcccggcg gattctttgc
      721 gccacccttc gtgccggcaa cacgcggaat gtgctcaagc agacgtagcc aaaggcggcg
      781 ccgacgagga gcgcgatata ggcgttcatg aaggccgcga agtttgagag aaactctggc
      841 tgaagggcaa gacccgccga gaagccgacc atagagccga gcgccatcgt cgcatatttc
      901 ggctgcgcga agaaaaagcc ggcaagaaac acaacaggga acaggaccag cgccagcatg
      961 acgaacccgt ctatcgactg caagatcgcg aattcataga aaaccgtgac gggaaacgcg
     1021 aggaaaaacc cgtagatcag agttccggcc aacggagtcg gatcgtccgc cgcaatgaaa
     1081 attgagcaca ggaccgacgt gattgcgatg gcggtcgcgc cactctccca tttcgtcgcg
     1141 atggtgaacg tcgaaatgcc gatgattgtc agcgcaacga cgatagcgga aaatagcgcg
     1201 aggccagcat cagtatgcaa tgtgcgcggc cgccgctcgc cgcacaagtc ctgcacctcc
     1261 ggatcgagac gcgtcggatt gcctagggcg gaggtaagga caagactttc ctgccaagcg
     1321 cgcgcaaggt ccgcaaggcg tgcgctcagg ctcgcacaca acaaatccgg ccattccgcc
     1381 gctgcatccg tccgcggatg tagccgcgcg atctcgtcca tgatccgcgc atagccggcc
     1441 ttgtccgccg attgcgcacc cttcagccac gcgacgaccg cacttacaat ttcctcgacg
     1501 atcggtggaa tcggccccgt ctcccgtagc gccttcagcc ggtcttccac ggcgctggtc
     1561 aagggtagaa gcgagacgag gcgctcttcg agagcgcgca gaacccgccg ggccgaacgg
     1621 ggaaagtctt cctcgaagcg caaattcgtc gccatgaggc tcagatccgt caagcctgtc
     1681 gcgatatgct ggtgtgccag cgcaggcccc agcaaggctg gctccgcgag cacgtccccg
     1741 atagaggttc gcgcatcggc gagaatttcg ttgagcttcc cctgcatgaa agtcgtgacg
     1801 ctgcgcggaa acagaatgct ttgaacgacc gcggacgcga gaacgccgat gacagtttct
     1861 tcagcgcgcg cgacgctgat attgaagagc gcggtcggat cagtcgcctg cggcaggccg
     1921 accagcgcca ccgtatatgc tgcaagcatg aagacataag cacgcggtgt ccgatcaagc
     1981 agcgcgccgt aaaggcacag gccgatccag ccggcgagcg cgagaatcat gagctccggc
     2041 gcctgcgaaa gattggggat gatcagcagc gacgcggccg cgccgatcag cgtgccaacg
     2101 atacggtaag ccgctttggc ccagatggcg ccgaccaaca attgcgtcgg ctgggcgaga
     2161 aaaaccgtga ccatcgccca ccacggattg gggagatcga gggcgagggc aatataaagc
     2221 gcgagtgctg tcgcgaggta ggcgttgata gcaaagatga aaggccgcgc acgaaagatc
     2281 cccatttgca acgcgactcc gcgcaaactc caatgtcatt gcacgagcta tgggaacata
     2341 aacctaagct gatgatcaag cactttgggg cacccgcccg taatcaggtg tgcttcggcg
     2401 gtgcgggggg caccggcaaa gcgatatccg tcgccatgcg ccagccttgt ggcgtcctga
     2461 tccattccac gatcatgagg aacggcttcg gcaccgggct ctggccggcg taggacaccg
     2521 atatctgtaa tggaatatag gtctcggcga cgtctttggt gagaccgacg attctttccc
     2581 gcgcatagtc gggagccata tgaaatgtgc ctttgtacag agcgctgaag tgtgccagca
     2641 cgacgccctt gccccagaat cccgcccaat tgccctgaga cggatcggac gttttggcaa
     2701 cgaaaagcgc cgagggcgaa tcccagacgt acggtgctac cgccctgacg tcgtgtgcat
     2761 tctcggcgtc aatgagttgt ttgtagagtt gccgcaactc cgtcaaagac gccgtgtcca
     2821 gcgggtcagt ggcggccttt gccccggtac taacgagcgt ggccgccact cccaacgagc
     2881 cgatgaaaat tcggaccaag ctttgcattg ccagcttccc tgccgctttt aggactggat
     2941 gccgaacggt tagcgacaat actgcggcgg cgccatcatc cagtcgtata gttactttgg
     3001 aacttaacgg ctgatgcccg cgcctaagag ttcaaatctt gcgggcaaac atttcggcct
     3061 gatgcttttt tagcgcgtct tcgatgacgg tcggaaacac attgcggaaa tgaccttgct
     3121 ccgcagggat cacctcgatc attttgttga tcatgtcctc ggga
//
LOCUS       DASWOG010000410         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_339028,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000410 DASWOG010000000
VERSION     DASWOG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339028"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="VGB93_08515"
     CDS             complement(<1..>1517)
                     /locus_tag="VGB93_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0146180.1"
                     /translation="PDDLLLCLQFLARHYERPSSETALTAGLPLVEGRLTPELVPTAA
                     ARIGLGAKILKRSLAELTPLYLPCIVFTSAGRAVALLECTAATCRVYAPDFDSIMEAP
                     RVEIEDHYSGLAITISPLFGRGAVDPGVPTALHQGHWFFGALAAHWRSFVSIGLAAAI
                     INIVGIATPLFTMNVYDRVLPNTAMATLWVLAIGFAVVLTFDLVLKTARALLLDTVGK
                     VLDIALSSAIFEKIMNTPLVARSNSTGEFVNRVTQYEFVREFFTSSTIVLFVDAAFVF
                     VYLIVIYAVAGWLVAIPIVAMMAVDGVGVLIQYRVGEEIGKAQTESSLRHAMLVEAVG
                     AIETIKTMRAEGQFLRRWDRLIRVASATQERIKSISSMGVNVTLFFQQLVTIGIIVAG
                     AYRFADREITTGAIIAAVMLSSRAVAPLTQIALMLTRLRYAISAMKTLNGIMNLQDER
                     VATESFVNRAVEHGKLEFRNVAFQYPLNTRLVLDGINLTISPGEKVGIIGKVGSG"
BASE COUNT          359 a          439 c          446 g          273 t
ORIGIN      
        1 tgccggaacc gaccttaccg atgatgccaa ctttctcacc aggcgatatc gtgagattga
       61 ttccgtcgag cacaagccgc gtattcaacg gatattgaaa tgcgacattg cggaattcga
      121 gcttgccatg ttcgacggcc ctattgacaa aactctcggt cgcgacacgc tcgtcctgaa
      181 gattcataat tccgttgagc gtcttcatcg cggaaatggc gtatcggaga cgcgtgagca
      241 tcagcgcaat ctgcgtgagc ggcgcgacgg cgcgggatga aagcatcacc gcggcgataa
      301 tggcgccggt ggtaatttcg cgatccgcga agcgataggc ccccgcgaca atgatgccga
      361 tcgtcacaag ctgctgaaag aacagcgtga cgttgacgcc catagaagat atcgacttga
      421 tccgctcctg cgttgccgag gcaacacgga tcagccggtc ccagcgcctt agaaattgac
      481 cttcggcgcg catggtcttg atcgtttcga tcgcgccgac agcctcgacg agcatcgcat
      541 ggcggagcga gctttccgtc tgtgctttac cgatttcttc gccaacgcga tattgaatca
      601 gcacgccgac accgtccacg gccatcatcg caacgatcgg gattgccacg agccagccgg
      661 cgacagcata gatgacgatc aggtagacaa agacaaacgc ggcatcgacg aacagcacga
      721 tagtgctcga cgtgaaaaat tcacgcacaa attcatattg cgtgacgcgg tttacgaatt
      781 cgcccgtcga attcgaccgt gccacgagcg gcgtattcat gatcttttcg aaaatcgcac
      841 tggagagcgc gatgtcgagg accttgccga ctgtatcaag gagaagcgcc cgcgcagtct
      901 tgagaacgag gtcaaacgtc aggacgacgg caaaaccgat tgcgagcacc cagagcgtcg
      961 ccatcgccgt attcggcagc acgcgatcgt agacgttcat cgtgaagagc ggcgtggcga
     1021 tgccgacaat attgatgatt gccgccgcga gaccgatcga tacaaaactc cgccaatgcg
     1081 ccgcgagagc gccgaagaac caatgccctt ggtgcagcgc cgtcggaaca ccgggatcga
     1141 ccgcgccacg gccgaagagt ggcgagatcg tgatggcgag accggaataa tgatcttcta
     1201 tctcgacccg cggcgcctcc atgatcgaat cgaaatccgg tgcatagacg cgacaggtcg
     1261 cggcggtgca ttcgagcaaa gcgacggcgc gcccggcgct cgtgaacacg atgcacggca
     1321 gatagagggg cgttagctcg gcgagtgacc gtttcagaat ttttgcgccg agaccgatgc
     1381 gggccgccgc ggtcggcacg agttcgggcg tcagccggcc ttcgacaaga ggcaaaccag
     1441 ccgtcagtgc ggtttccgac gacggccgct cgtaatgacg cgccagaaat tgcagacaga
     1501 gcagcaaatc gtccggc
//
LOCUS       DASWOG010000411         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_321364,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000411 DASWOG010000000
VERSION     DASWOG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321364"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGB93_08520"
     CDS             <1..306
                     /locus_tag="VGB93_08520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0146181.1"
                     /translation="ELDDIGHSIAAMERAGFEVQDVEGWRLHYARTCQLWCERLTANK
                     EQAIQYVGEEKYRIWVAYLAGVSLAFSRGTLRLFQTLASKSAKRTPMLPPTRADLYR"
     gene            complement(510..1466)
                     /locus_tag="VGB93_08525"
     CDS             complement(510..1466)
                     /locus_tag="VGB93_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY0146182.1"
                     /translation="MDMKLHANARTTPRTRAEIQLRAKTTPVAVLATEYGVNEATIRR
                     WKDRDSVADRSHTAKRLAIAMTPAEEALAVELRTALALPLDDIVEVMRRCANPKLSRS
                     GIHRCLRRHGVAKRTKPQEDRQAAGMFETAPVGFIHIDLKHLTRLEGRPAFVFVAIDR
                     ATRFVHIEIITRRDAGTIALCLERFLAAFPHKVHTILTDNGAEFTDRFREARWGRKTR
                     ASGRHAFDRVCAVNDIEHRLTRPFHPQTNGMVERFNRRIGEAVVAKTSVTQNEGKNKF
                     RTHAERDAFLHDFVENYNRTRLRCLDYKAPAALIANPPGHNT"
BASE COUNT          269 a          467 c          509 g          299 t
ORIGIN      
        1 gaactcgacg acatcggcca ttcgattgcc gcgatggaac gcgcgggctt cgaggtgcag
       61 gacgtcgaag gctggcgcct ccattacgcc cgcacctgcc agctctggtg cgagcgcctg
      121 accgccaaca aggagcaggc gattcaatac gtcggcgaag agaaataccg gatctgggtc
      181 gcctacctcg caggcgtcag cctcgcgttc tcgcgcggca cgctgcggct gttccagacg
      241 ctcgcctcga agtctgcaaa gcgaacgccg atgctgccgc cgacccgcgc cgatctttat
      301 cgctgagcta gctctgcaaa aaaactttgt catcccggcc gaagcgcagc gaagagccgg
      361 gacccagggc cggcatcgac gctgggatct gggtcccggg tctcgcaagt gggctcgccc
      421 gggatgacaa acttcggctt gattagtccg tgatccggcc atccagggcg cttgctgagg
      481 ccgcaaggcg cctggtcgtg gatggccgcc taagtgttgt gtcccggcgg attggcgatg
      541 agtgcggctg gtgccttata atcgaggcat ctgaggcgtg tgcgattata gttttcgacg
      601 aagtcgtgga ggaaggcatc tcgctcggca tgggtgcgga atttgttctt gccctcattc
      661 tgggtaacgc ttgtcttggc tacgacagcc tctccgatcc gacgattgaa gcgttcaacc
      721 atgccgttcg tctgaggatg gaaaggacgc gttaaacgat gctcgatgtc gtttactgcg
      781 cacacgcgat cgaaggcgtg ccgtccgctc gctctcgtct ttcgccccca acgagcttcg
      841 cggaagcgat cagtgaactc cgcgccgttg tcagtgagga tagtgtgcac cttgtgcgga
      901 aaagcagcga ggaaccgctc aagacagagc gcaatcgtcc cggcatcgcg gcgcgtgatg
      961 atctcgatgt gtacgaagcg cgtggcgcgg tcgatggcga cgaacacaaa ggccggacgg
     1021 ccttcgaggc gcgtcaggtg cttcaggtcg atatggatga agccgaccgg cgccgtctcg
     1081 aacatgccgg ccgcttggcg gtcttcttgc ggcttggtcc gcttcgcgac cccgtggcgc
     1141 ctgaggcaac ggtggatgcc gctgcgcgac agcttgggat tggcgcagcg gcgcatgacc
     1201 tcgacgatgt cgtcgagcgg cagagcaagc gcggtccgca gttcgaccgc cagcgcctcc
     1261 tcggcgggcg tcatcgctat ggcaaggcgc ttggccgtgt gagagcggtc ggcgacgcta
     1321 tcgcgatctt tccagcggcg gatggtggct tcgttgacgc catactcggt cgcgagcaca
     1381 gctaccggag tggtcttggc gcgcaattga atttcggccc gggtgcgcgg cgtggtgcgg
     1441 gcgttcgcgt gcagcttcat gtccatcggc cgaggcgctc cctgacgaag gcaatgcctc
     1501 tcgctacacc gattccgtca cgccaatccc ccgggacgaa acac
//
LOCUS       DASWOG010000412         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_27906,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000412 DASWOG010000000
VERSION     DASWOG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27906"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1915)
                     /locus_tag="VGB93_08530"
     CDS             complement(53..1915)
                     /locus_tag="VGB93_08530"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0146183.1"
                     /translation="MFESHYQSFTDTADASQSAVRLAALRTELKKHWLDGFIVPRADR
                     HQNEYVPPSEERLAWLTGFTGSAGLAIVLQDRAALFVDGRYTIQVREQVDLEAVEPVA
                     LATTSPEKWLEGNLHTGAKLGYDPWLHTPGQIERYEKAAKTAGAELVAVEQNPIDAIW
                     TDRPAPPRGAVKRHPPKLTGESAKKKLARVTTALGKADALLISDPHAVAWVFNIRGQD
                     VDHTPLPLAFALITRDGPPRLYIEAAKLNSNVRAYLEEHTEIADPAALEDDLVRLGVE
                     TKRVLFDSATVPTRLTRLLKEAGGAADVGADPIALMKARKNLTELEGTRAAHLRDGIA
                     MVRFLRWFSEEAPKRKLTEIAAAQALETFRRESGQLKDVSFPTISAAGPNSAIPHYRV
                     TEATNRKIRRGIFLVDSGAQYEDGTTDITRTLAVGAPTQEMRDRFTRVLKGHIAIARA
                     LFPRGTSGAQIDAFARLPLWQAGLDFDHGTGHGVGSYLSVHEGPQRISKLSNVALEPG
                     MILSDEPGYYKAEHFGIRIENLVVVTPVKIRGAERDMLGFETITFAPIDRALVEPKLL
                     DAGEITWLDAYHARVRKLLSPHLDRDEREWLKQATQPINNAKPAKRHKGKHGRH"
     gene            complement(1964..>2429)
                     /locus_tag="VGB93_08535"
     CDS             complement(1964..>2429)
                     /locus_tag="VGB93_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEY0146184.1"
                     /translation="TCAMTAPLRLIALDTEDLAIMSAHLQDSLVTVGDMAYLPRSKRF
                     AAVAARFDWVAEAEGKKERCETGFHFERVLKVSRSGFSQQDPAQRLMLLSVAFAETDS
                     PAGQVVLTFSGGAAIKLDVECLEAQVQDLGRRWTCTACPNHDLEEVSQKQAS"
BASE COUNT          449 a          709 c          765 g          506 t
ORIGIN      
        1 gctttccttg gcgcgataat gcgcaaccca gaaggcagta tccgagacgt tttcaatgcc
       61 ggccatgctt gcccttgtga cgctttgccg gctttgcgtt gttgatcggc tgcgtggcct
      121 gcttcagcca ttcacgttcg tcgcgatcga gatgcggcga gagcagttta cgaacccgcg
      181 catggtaagc gtcgagccac gtaatttcac ctgcatccag caattttggt tcgacaagcg
      241 cacgatcgat cggcgcgaag gtgatcgtct caaatccgag catgtcgcgc tctgcgcccc
      301 tgatctttac cggcgtgacg acgacgagat tttcaatgcg aatgccaaaa tgctcagctt
      361 tgtaatagcc tggctcgtcc gagaggatca tgcccggttc gagtgccaca ttgctgagtt
      421 tcgagatgcg ctgcggtcct tcatggaccg atagatagga gccgacgccg tggcccgtgc
      481 cgtgatcgaa atcgaggccc gcctgccaaa gtggcagtcg tgcaaaggca tcaatttgcg
      541 cgcccgacgt gcctcgcgga aacaaggcac gggcgatggc gatgtgccct ttgagcacgc
      601 gcgtgaaacg gtcgcgcatt tcctgcgtcg gcgcgccgac cgccagcgtg cgcgtaatgt
      661 cggtcgtgcc gtcttcgtat tgcgcgccgg aatcgacgag gaaaatgccg cgcctgatct
      721 tgcggttcgt cgcttccgtc acgcgataat gcgggatcgc tgaatttggg cccgcggctg
      781 aaatcgtcgg gaaggaaaca tctttcaatt ggccgctctc gcggcgaaag gtttcaagtg
      841 cctgtgccgc tgcgatctcg gtcagcttgc gtttgggcgc ttcctcggaa aaccagcgta
      901 gaaagcgaac catcgcgatg ccatcgcgga gatgcgcggc acgcgtgccc tcaagttcag
      961 tgagattttt acgcgccttc atcagcgcga tcggatctgc gccaacgtcc gcggcgccgc
     1021 cggcctcttt aagaagccgc gtcagtctcg tgggtaccgt cgcggaatcg aaaagaacgc
     1081 gtttcgtttc gacgccaagg cgaaccaaat cgtcttcgag tgctgccggg tctgcaatct
     1141 cggtgtgctc ttcgagatag gcgcgcacat tagaattcag tttcgccgct tcgatataca
     1201 ggcgcggtgg gccgtcgcgc gtgatgagcg cgaaagccag cggcaggggc gtgtgatcca
     1261 catcctgtcc gcggatgttg aacacccacg ccaccgcatg cggatcgctg atcagtaagg
     1321 cgtcggcctt gccgagcgct gtggtcacgc gtgcaagttt tttcttggcc gactcgccag
     1381 taagctttgg cggatggcgt ttgacggcgc cgcgtggggg cgcgggtcgg tccgtccaga
     1441 ttgcgtcgat cggattttgt tcgacagcaa cgagctccgc gccggcggtc ttggcggctt
     1501 tttcgtagcg ttcgatttgc cccggcgtat gaagccaagg gtcatagccg agctttgcgc
     1561 ctgtgtggag attgccttcg agccattttt cggggctcgt cgtggcgaga gcgaccggtt
     1621 caacagcctc caagtcgact tgctcgcgca cctgaatcgt atagcggcca tcaacaaaga
     1681 gagccgcgcg atcttgcagc acgatcgcaa ggcccgccga gccagtgaag ccggtaagcc
     1741 aggccaaccg ctcctctgag ggcggcacat attcgttctg atgccggtcg gcgcggggca
     1801 cgatgaagcc atcgagccag tgctttttca attcggttcg caatgccgcg agccgcaccg
     1861 cggattgcga cgcgtcggca gtgtcggtga aggattggta gtggctctcg aacacgcggg
     1921 ttagcttagc cgaattgtgc ggagatttcg attgcggcga aaactaactc gcctgtttct
     1981 gcgagacttc ctcgagatcg tggttcgggc aggccgtgca ggtccaacgg cgtccaaggt
     2041 cttgcacctg ggcttcgaga cattcgacat cgagtttgat ggcggcaccg ccggaaaacg
     2101 tcaggacgac ttgccccgca gggctatcgg tctccgcgaa cgcgacgctc agaagcatca
     2161 agcgctgcgc cgggtcctgc tgggagaagc cgctgcgcga aaccttcaaa acccgctcaa
     2221 agtggaagcc ggtctcgcag cgctccttct tgccttcggc ctcggcaacc cagtcgaagc
     2281 gggcggcaac cgccgcaaag cgcttggagc gcggcagata agccatgtcg ccgacggtga
     2341 cgagcgagtc ctgcagatgc gcggacatga tcgcgagatc ttcggtgtcg agagcgatca
     2401 gacgcagcgg ggccgtcatg gcgcaggtt
//
LOCUS       DASWOG010000413         5969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_357375,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000413 DASWOG010000000
VERSION     DASWOG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357375"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1452
                     /locus_tag="VGB93_08540"
     CDS             40..1452
                     /locus_tag="VGB93_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0146185.1"
                     /translation="MKEISRFGIVVALTLGSALAVAPLAADPAVPTSAGEITLSFAPI
                     VKKVAPAVVNVYASRTDVQPRNPLFDDPVFRHFFGDSQEQRPGGPTAQSLGSGVLIDP
                     SGLVVTNYHVIAGMTDVKVALSDRREMPAKIVLRDQRTDLAVLRLDGKGPFPYMEFDD
                     SDKLEVGDLVLAIGDPFGVGQTVTQGIISALARTEEGISHYGFFIQTDAAINPGNSGG
                     ALVDMRGRLIGINSAIVSQTGSSVGIGFAIPSDMVKVIVATAKSGRTEVRRPWLGAGL
                     QNITKDIADSLNLDRPVGTLVTDVTAKGPAEKAGLQHGDLIVAVDGQPTDDLEAFSYR
                     FATVPLGNNAVFSVIRDGKSLKLTIKAEPAPELPRDPVKIAGSSPFAGATVINISPAV
                     AEEFSLESAQSGVVISDIDSDSRAAVVNLQKGDVVIAVNNTKINTTHDLEAATAGEHY
                     FWKVSIGRGNQIITTVVGGN"
     gene            complement(1490..2149)
                     /locus_tag="VGB93_08545"
     CDS             complement(1490..2149)
                     /locus_tag="VGB93_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0146186.1"
                     /translation="MRVVAMVNQKGGSGKSTLAACLAVAAQESGERVFLLDMDPQKSL
                     LKWAHRRQDENMLCEAVTPAKLEAAVAALARGRVTFVIIDTPATDSPAADAAMRVADL
                     CLIPARPTIFDIWASEATRGKLKTLAKDYAFILNQCPPMQESQRVNDGAAAIEAMGGL
                     LRPFIVARVDYQEAAREGMGATEIDAEGKAAEEISHLWSSLKRRLSRLKPAEKSGRRA
                     A"
     gene            2321..3628
                     /locus_tag="VGB93_08550"
     CDS             2321..3628
                     /locus_tag="VGB93_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0146187.1"
                     /translation="MSDLFAAAHLSDQAPHPLADRLRPQHLDEVAGQDHLLAPEGALG
                     RLIRAGSLGSLIFWGPPGTGKTTVARLLAAETKLAFVQISAIFTGVAELKKVFEEARK
                     RRAIGQGTLLFVDEIHRFNRTQQDSFLPVMEDGTITLIGATTENPSFELNAALLSRAR
                     VLTFRALDEPALDKLLTRAETLEGKELPLTPDARGALLRMADGDGRAILTLAEEVWRA
                     APPAPLDVESLTEIVQRRAPIYDKAQEGHYNLISALHKCVRGSDPDAALYYLARMLDA
                     GEAPLFIARRIVRMAIEDIGLADPQALVVANAAKDAYDFLGSPEGELALANALIYVAT
                     APKSNAAYRAYGAAQRLAREHGSLMPPKVILNAPTKLMKTEGYGSGYEYDHDAPEAFS
                     GQDYWPEKLGRQDIYRPVERGFEREIKKRLEYWEKLRKERGRG"
     gene            3775..4824
                     /locus_tag="VGB93_08555"
     CDS             3775..4824
                     /locus_tag="VGB93_08555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0146188.1"
                     /translation="MIHRTVQTLLVGEDEDGLRLDRWFKRRLPTLSLSHLNKIVRTGQ
                     VRVDGARATTSTRLVTGQKIRVPPVNVVPPAPKPAFNEADRNAIRDMILYEDQDLMVL
                     NKPYGLAVQGGSGTKYHVDGMLASLADEKGERPVLVHRLDRDTAGVLLIAKSRRVAAK
                     LGEIFRSRQAKKIYWALVEGVPKPAQGRISLFLAKGEGMGDLRGAKSPSAHANREKMQ
                     IAKHGDAEAQHSVTYYAVVDKAAPRLAWLSMKPITGRTHQLRAHAEAIGHPIVGDPKY
                     SGDANTPRRLDPIRQIPDGVEHKLHLLARRLVLPHPRGGTLDVTAPLPPHMQKSFDMF
                     GFDVKGRDPIEDAPE"
     gene            complement(4825..>5969)
                     /gene="maeA"
                     /locus_tag="VGB93_08560"
     CDS             complement(4825..>5969)
                     /gene="maeA"
                     /locus_tag="VGB93_08560"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HEY0146189.1"
                     /translation="GKLALYTACAGTPPQYCLPITLDVGTNNPELLEDPLYLGLNQKR
                     VRGAEYAAFIDEFVEAVQSLYPKCCIQWEDFANFNAVPILELYRDKICTYNDDIQGTA
                     AIALAGIFAALRITKQKLTDQRFLFLGGGSAATGIAELISEAMTLEGLDFKTARARNA
                     LFDINGLVVRTRNDLADFQRPFALDHAPISKFVDAVKELRPTGIIGVSTVPKLFNQAV
                     IETMADINKRPIIFPYSNPTSRSECTAEEAYRWSQGRAIFASGSPFPPVEIEGQSFTP
                     GQGNNVYIFPAMGMAIFATEAKRVTEAMFIVAAKAVAEQVTEKNLADGLIYPPQSKIL
                     EASLHVAARIAEYIFDHDLAGVPRPPDISAHIRNRAYRPVYGTYAE"
BASE COUNT         1145 a         1787 c         1875 g         1162 t
ORIGIN      
        1 cgctaaatcc gggtgattgg tggaattgac gaggaccgca tgaaagagat ttcgcgcttc
       61 gggattgttg tggctttgac tctcggctcg gctcttgccg tagcgcccct ggcggctgat
      121 cctgccgtgc cgacatcggc cggcgagatc actttatcct tcgcgccgat cgtcaagaag
      181 gtcgcgcccg ccgtcgtcaa tgtttacgcg tcgcgtaccg acgttcagcc gcgcaatcct
      241 ttgttcgacg atccggtctt ccggcatttc tttggcgatt cgcaggagca aagaccaggc
      301 ggcccgaccg cgcaatcgct cggctccggc gtgctgatcg atccgagtgg ccttgtcgtc
      361 accaattatc atgtgatcgc gggcatgacc gacgtgaagg tcgcgctctc agaccgtcgc
      421 gaaatgccgg cgaagattgt gctgcgcgat cagcgcaccg atctggctgt gctgcggctc
      481 gatggtaaag gaccgttccc gtatatggag ttcgacgatt ccgacaagct tgaggtcggc
      541 gatctcgtgc tcgcgattgg cgatcctttc ggtgtcgggc agaccgtgac gcaaggcatc
      601 atctcggcac tggcgcggac ggaagaaggc atttcgcact acggcttctt cattcagacc
      661 gatgcggcga tcaatcccgg caattccggc ggcgcgttgg tcgatatgcg tggccgcctc
      721 atcggcatca attcggcgat tgtctcgcag actgggagct cggtcggcat cggcttcgcg
      781 ataccctccg acatggtgaa ggtcattgtc gcgacggcaa aaagcggccg cacagaggtt
      841 cggcggccgt ggctcggcgc gggcctgcaa aacatcacca aggatatcgc cgattccctc
      901 aatctcgacc gcccggtcgg cacgctcgtc accgacgtga cggcgaaggg gcccgctgag
      961 aaagcgggac ttcagcatgg cgacctgatc gtcgctgtcg acggtcagcc cacggatgat
     1021 ctcgaagctt tcagttaccg cttcgcgact gtaccgctcg gcaacaatgc tgtcttctcg
     1081 gtgatccgcg acggcaaatc gctcaaactc acgatcaagg cggagccggc accggaactg
     1141 ccgcgagatc cggtgaaaat cgccggatcg tcgcccttcg ccggcgcgac ggtcatcaat
     1201 atttcgccgg cggtggcgga agagttttcg ctggaatcag cgcagagcgg cgtcgtcatc
     1261 agcgatattg atagcgactc gcgcgcggcg gtggtcaacc tgcagaaggg tgacgtcgtg
     1321 atcgcggtca acaatacaaa aatcaatacg acgcatgatc tggaggcggc gacggctggt
     1381 gagcattatt tctggaaagt cagcatcggc cgcggcaacc agatcatcac cacggttgtc
     1441 ggtggcaact aagcgaagca atcgtaggat tgcttcgtca gctttgaact caggcggcgc
     1501 gacgtccgct cttttccgcc ggcttcaggc ggctgaggcg gcgcttcaat gacgaccaga
     1561 ggtgggagat ttcttcggcg gctttgccct cagcgtcgat ttcggttgcg cccatgcctt
     1621 cgcgcgccgc ttcctgataa tcgacgcgcg cgacgatgaa ggggcgcagc aagccgccca
     1681 tcgcctcgat agcggcggca ccgtcgttga cgcgctggct ttcctgcatg ggcggacatt
     1741 ggttgaggat aaaagcgtaa tccttcgcaa gcgttttcag cttgccgcgg gtcgcttcgc
     1801 tcgcccagat atcgaagatc gtcggccggg cggggatcag gcaaagatcg gcgacgcgca
     1861 tggcggcatc agccgcgggg gaatccgtcg ccggggtgtc gatgatcacg aacgtaacgc
     1921 gcccgcgggc cagagccgcg acagcggcct caagcttcgc cggcgtcacc gcttcgcaca
     1981 gcatgttctc gtcctggcgg cgatgagccc atttcaacaa tgatttctga gggtccatgt
     2041 cgaggaggaa aacgcgttcg ccgctttcct gcgccgcgac ggcgaggcag gctgccagcg
     2101 tgctcttgcc cgatccaccc ttttgattga ccattgcgac gacacgcatg cgcgatgctc
     2161 ccgacattcg actcagaata tacttccttt caattctcat cgaaacgtta accgattgat
     2221 aaagatttgg atttcgtgtc ggagcgagga cgtgcgatga tacgtgcgga tgcgtttttg
     2281 cccttcattt ccgaggcttt cgggcccaaa ttgaagcaat atgtccgatc ttttcgccgc
     2341 cgctcatttg tctgatcaag ccccgcatcc gctggccgat cgtctgcgtc cgcaacatct
     2401 cgacgaggtc gccgggcagg atcatttgct ggcacctgaa ggggcattgg gccgtctgat
     2461 ccgcgccggt tcgctcggca gcctgatctt ctgggggccg cctggcaccg gcaagacgac
     2521 cgtcgcgcgg cttctggcgg cggaaaccaa gctcgctttc gtgcagattt cggcgatttt
     2581 tacgggtgtc gccgagctta agaaggtgtt cgaggaagcc cgcaagcgcc gtgcgatcgg
     2641 gcagggcacg ttgctcttcg tcgatgaaat ccatcgcttc aaccgcacac agcaggacag
     2701 cttcctgccg gtaatggaag acggcacgat cacgttgatc ggcgcgacaa cggagaatcc
     2761 gagcttcgaa ctcaacgccg cgcttctgtc acgcgcacgg gtgctgacgt ttcgtgctct
     2821 cgacgagccg gcgctcgata agcttctgac tcgagccgag acgcttgagg gcaaagagct
     2881 gccactgacg ccggacgccc gcggggctct tctacgcatg gcagatggtg atggccgcgc
     2941 catcctgacg ctcgccgagg aagtctggcg cgcggcaccc cccgcgccgc tcgatgtcga
     3001 atccctcacc gagatcgtgc agcggcgtgc gccgatctac gacaaggctc aggagggcca
     3061 ttacaatctc atcagcgcgc tacataaatg cgtgcgcggt tcggaccccg atgcggcgct
     3121 ctattatctc gcgcggatgc tcgacgcggg cgaggcgccg ctcttcatcg cccggcgcat
     3181 tgtgcggatg gcgatcgagg atattggcct tgccgacccg caagcgcttg tcgtcgcgaa
     3241 cgcggcgaag gacgcctatg atttcctcgg ctcgccagag ggcgaactgg cgttggccaa
     3301 tgcgctgatc tatgtcgcga cagcgccgaa gtcgaatgcc gcctatcgcg cctacggcgc
     3361 cgcgcagcgt cttgccaggg agcatggttc tctgatgccg ccgaaggtca ttctcaatgc
     3421 gccaacgaaa ttgatgaaga ccgagggcta tggctcgggc tacgagtacg atcacgacgc
     3481 gccggaggcg ttctcgggcc aggactattg gccggaaaag ttgggtcgcc aggacatcta
     3541 tcgtcccgtc gagcgcggct tcgaacgcga gatcaaaaag cggctcgaat attgggaaaa
     3601 gctcaggaag gaacggggac gagggtgaaa aaagcacctg caaaggttcg ggcgaaaagt
     3661 ctccccgcgg gagaaagcgg cggcttgcgc ggcaagccag gcgtcgcacg aacgccgaag
     3721 acggagccct ctcgcccggc cggcgctggc gtccaacccc tcgccgagga agaggtgatc
     3781 catcgcaccg tccaaaccct cctggtcggc gaagatgagg acgggctgcg cctcgaccgc
     3841 tggttcaagc ggcggctgcc gacgctctcg ctctcgcatc tgaacaagat cgttcgcacc
     3901 ggccaggtgc gtgtcgatgg cgcccgcgcg acgacatcaa cgcggctggt taccgggcaa
     3961 aagattcggg tccctcctgt caatgtcgta ccgcccgcgc cgaagcccgc attcaacgag
     4021 gccgaccgca acgccatccg cgacatgatc ctctatgagg atcaggatct gatggtgctc
     4081 aacaagccgt atgggcttgc ggtgcagggc ggctcaggca ccaagtatca tgtcgatggc
     4141 atgctggcct cgctcgcgga cgaaaagggc gagcgcccgg tgctcgtgca tcggctcgac
     4201 cgcgatacgg cgggcgtctt gctcatcgcc aaatcgcgtc gcgttgccgc gaagctcggg
     4261 gagatttttc gctcacgtca ggcgaagaaa atctattggg ctctcgtcga aggcgtgccg
     4321 aagcccgcac aggggcgcat ctcactattt ctcgccaagg gcgagggcat gggcgatctg
     4381 cgcggagcca aatcgccaag cgcgcacgcc aatcgcgaga agatgcagat cgccaagcat
     4441 ggcgatgctg aagcgcaaca ttcggtgacg tattacgcgg tggtcgataa agcggcgccg
     4501 cggcttgcgt ggctctcgat gaagccgatc actggccgca cgcatcaatt gcgcgcccat
     4561 gccgaagcca tcggccatcc gattgtcggt gatccaaaat attccggaga cgccaacacg
     4621 ccgcgccgtc tcgatccaat acggcaaatt ccggatggcg tcgaacacaa gctgcatctc
     4681 ctcgcgcgcc ggctcgtgct gccccaccca cgcggcggaa cgctcgatgt gaccgcgccg
     4741 cttccgccgc atatgcagaa gagcttcgac atgttcggct tcgacgtgaa aggccgggac
     4801 ccgatcgaag acgcgcccga ataattattc ggcgtaagtg ccgtagaccg gccgataagc
     4861 gcggttgcga atgtgggccg agatgtcggg cgggcgcggc acgccggcaa ggtcgtgatc
     4921 gaagatatat tccgcaatcc gcgccgcgac atgcagcgag gcttcgagaa ttttgctctg
     4981 cggcggataa atcaagccgt ccgcgaggtt tttctcggtc acctgttcgg cgacagcttt
     5041 cgccgcaacg atgaacattg cctcagttac gcgcttggct tcggtggcga agatcgccat
     5101 gcccatcgcc gggaagatat agacgttgtt accctggccg ggcgtaaagc tctggccctc
     5161 gatctcgacg ggtgggaacg gacttccgct ggcgaaaatt gcgcggccct gcgaccagcg
     5221 ataggcttcc tccgccgtac attccgagcg cgacgtcggg ttggaataag gaaagatgat
     5281 cggccgcttg ttgatatccg ccatggtttc gatgacggcc tgattgaaca gtttcggcac
     5341 cgtgctgacg ccaatgatcc ccgtcgggcg cagttctttg accgcgtcga cgaatttgga
     5401 gatcggcgca tgatcgagtg cgaagggccg ctgaaaatcg gcgaggtcat tgcgcgtacg
     5461 caccacaagg ccgttgatgt cgaagagggc gttgcgcgca cgcgccgtct tgaagtcgag
     5521 cccttcgagc gtcatcgcct cgctgatgag ttcggcgatg ccggtcgccg ctgagccgcc
     5581 gccgaggaaa agaaatcgct ggtcggtgag tttctgcttc gtgatccgca acgcggcaaa
     5641 aatgccggcg agtgcaattg ccgcggtgcc ctgaatgtca tcgttatacg tgcagatctt
     5701 gtcgcgatag agttcgagaa tcggcacggc gttgaagttg gcgaagtctt cccattgaat
     5761 gcaacacttg ggataaagcg attgcaccgc ttcgacgaac tcgtcgatga aggcggcata
     5821 ttcggcgccg cgcacgcgct tctgattgag gccgagatag agcgggtctt cgagcaattc
     5881 cggattgttg gtcccgacat caagcgtgat cggcaggcaa tattgcggcg gcgtgccggc
     5941 gcaggcggtg tagagcgcga gcttgccga
//
LOCUS       DASWOG010000414         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_119407,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000414 DASWOG010000000
VERSION     DASWOG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119407"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VGB93_08565"
     CDS             <1..1355
                     /locus_tag="VGB93_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0146190.1"
                     /translation="PIYFVTRETWPNIEASLPAAAAHFAASSKFEPTPGACLTLPDAD
                     GRIASVLFGLEAQSARHRDPFLPGKLVAALPADVYRFANAPHDTPLAVLAWALAAYRF
                     DRYKKKKQDQPRLVPPDHIDLVRIKTLAAGVTLGRDLINTPANDLGPDALEVAAVTLA
                     AEFGAEAAITLGDDLLKENFPLIHAVGRAAEKPPRLVDFSWGPEGAPKVTLVGKGVCF
                     DTGGLDIKPSSGMILMKKDMGGAATALALAHFIMDRGLPIRLRVLLPIVENAISGSAM
                     RPSDVYPSRKGLSVEIGNTDAEGRLILADALTLADEGAPELLFDFATLTGAARVALGP
                     DLPPFYTEDETLAAEIAKFAHTANDPVWRLPLWAPYDNLIDSKTADVSNSGTSGFAGS
                     ITAALFLKRFVENTKSWVHFDIYAWTPSAKPGRPEGGEIQVARLLFDLLESRYGARAS
                     "
     gene            1352..2200
                     /locus_tag="VGB93_08570"
     CDS             1352..2200
                     /locus_tag="VGB93_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0146191.1"
                     /translation="MSSFDARVTPARPDLAAAHLRGKVEAPRFVNGRPMHLRSEIADL
                     RREPSLEAPIDTQILFGEAVTLYDEHEGWAWVQLASDNYVGYMSRDALAEGGPAPTHR
                     VGVNRTFVYPAANMKLPLHGALPRGAALRVSETIGDFARIGGGFVFAAHLAPLADPAD
                     DFVAVAEDYLGSPYLWGGKTSLGIDCSGLVQIALAAAGHAAPRDTDLQEAALGRVLPI
                     DDKLEQLQRGDLVFWKGHVGIMRDPRTLLHANGHHMLVVSETLAEARTRIRQKGAGEI
                     TSIKRL"
     gene            complement(2187..2636)
                     /gene="cdd"
                     /locus_tag="VGB93_08575"
     CDS             complement(2187..2636)
                     /gene="cdd"
                     /locus_tag="VGB93_08575"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0146192.1"
                     /translation="MNVISENKISQAALDKLFAAALTARENAYAPYSHFAVGAAIATP
                     DGQIFAGGNVENAAYPAGCCAETGAITAMVAAGGRQISDVLVVADCDRGILPCGSCRQ
                     RIAEFATEDAVVHSAGPDGVHKSVKFAALLPEAFDRQTLESESARAV"
     gene            complement(2696..2986)
                     /locus_tag="VGB93_08580"
     CDS             complement(2696..2986)
                     /locus_tag="VGB93_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146193.1"
                     /translation="MSIEALYPSRRHPQHDNPVLRRERRQQRVIAALALGFCLISMAA
                     LRISPNEAVAEARMPIVQKTVVVEAPVHHHIRVIPLYNIPPEQRGGVFADEN"
     gene            3142..3540
                     /locus_tag="VGB93_08585"
     CDS             3142..3540
                     /locus_tag="VGB93_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HEY0146194.1"
                     /translation="MDGQAAARVSVEINFPNGGVFGREEILLINAIRAQRSIIGASRA
                     LGLSYRKCWLVVDALNRTFESTVIATYPGRRGSGAEITAFGERLVALYQSIERHAGTS
                     AERSLAEVTASLDWNFDPKARSAVAGRGLA"
     gene            complement(3470..3919)
                     /locus_tag="VGB93_08590"
     CDS             complement(3470..3919)
                     /locus_tag="VGB93_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0146195.1"
                     /translation="MLETTVFARILSHDPDLALTSLLFDDGELRLPMVDLPVGSSVVV
                     KIDARDVSIALSRPMDVSITNRLPGAIDEIEFLAPPYVRATFSLGKTRLHSLITRESV
                     GRLALVPGLKAWAMIKAVAISGRGVRPDRAPRPRSWPSDRNSSRETP"
BASE COUNT          742 a         1251 c         1221 g          719 t
ORIGIN      
        1 tgccgattta tttcgtcacg cgggagacct ggccgaacat cgaagcgagt ttgccagcgg
       61 ccgctgcaca tttcgccgcg agctcgaaat tcgagccgac gccaggcgcg tgtctcaccc
      121 ttccggacgc cgatggccgg attgcgagcg tgctcttcgg acttgaagcc caatcggcgc
      181 gtcatcgcga cccgttcctg cccggcaagc tggtcgccgc gctgccggcg gacgtctatc
      241 gttttgccaa tgcgccacac gatacgccgc ttgccgtcct cgcctgggcg ctcgctgcct
      301 atcgattcga tcgctacaaa aagaagaaac aggatcagcc gcgtctcgtg ccgccggacc
      361 acatcgatct cgtacggatc aagacgcttg ccgccggcgt gacgcttggg cgcgacctca
      421 tcaatacgcc ggcaaatgat cttggccccg acgccctcga agtggcggcc gtaacgcttg
      481 ccgcggaatt cggggccgaa gccgcgatca cgctgggcga cgatctgctg aaggaaaact
      541 ttccgctgat ccatgccgtc ggccgcgcgg cggagaagcc gccgcggctc gttgatttct
      601 catggggacc ggaaggcgcg ccaaaagtga cgcttgtcgg caagggcgtc tgtttcgata
      661 ccggcggtct cgacatcaag ccgtcgagcg gcatgatcct gatgaaaaag gatatgggcg
      721 gggcggcgac ggcgctggcg ctggcgcatt tcatcatgga ccgcggcctg ccaattcggc
      781 tgcgcgtgct gctgccgatc gtcgagaacg cgatttccgg cagcgccatg cggccaagcg
      841 atgtctaccc tagccgcaaa ggcctgagcg tcgaaatcgg caataccgac gccgagggcc
      901 ggctaatcct cgccgacgcg ctgacgctcg cggatgaagg cgcgcccgaa ctcttgttcg
      961 acttcgcgac gctgaccggc gccgcccgcg tcgcgctcgg ccccgatctg ccgcccttct
     1021 ataccgaaga cgagaccctg gccgccgaga tcgcgaaatt cgctcacact gcgaacgatc
     1081 ccgtctggcg gctcccgctc tgggcgcctt atgacaacct gatcgacagc aagacggccg
     1141 atgtcagcaa cagcgggacc agcggctttg ccggttccat caccgccgcg ctcttcttga
     1201 agcgcttcgt cgaaaacacg aaatcatggg tgcatttcga tatttacgcc tggacgccga
     1261 gtgcgaaacc gggtcggccg gaaggcggtg aaatccaggt ggcgcggctt ctctttgatt
     1321 tgctcgaaag ccgctacgga gcgcgcgcgt cgtgagcagc ttcgatgcgc gagtgacgcc
     1381 ggcgcggccc gatctggcgg ccgcgcattt gcgcggcaag gtcgaagcgc cgcgctttgt
     1441 gaatggcagg cctatgcatc tacgttccga aatcgccgat ctacgccgcg agccatctct
     1501 cgaggcgccg atcgatacgc aaattctctt cggcgaagcg gtgacgcttt acgatgagca
     1561 cgaaggctgg gcctgggtgc aactcgcaag cgacaattac gttgggtaca tgtcgcgcga
     1621 tgctctggcc gagggtgggc ccgcgccgac gcatcgcgtc ggcgtcaatc gcacctttgt
     1681 ctatcctgcg gcaaatatga aattgccact ccatggggcc ttgccacgcg gcgcggcctt
     1741 gcgcgtcagc gagacaattg gtgatttcgc tcggattggc ggcggcttcg tcttcgccgc
     1801 gcatcttgcg ccgcttgccg accccgcaga cgatttcgtc gcagtggcgg aggattatct
     1861 tggcagcccc tatctctggg gcggcaagac gagcctcggc attgattgtt ctggcctcgt
     1921 gcagatcgcg ctcgccgcag caggccatgc cgcgccccgc gacaccgatc tacaagaagc
     1981 cgcgctcggt cgcgtcttgc ccatcgacga taagctcgaa caattgcaac gcggcgatct
     2041 cgtcttctgg aaaggtcatg tcggcatcat gcgcgacccg cgcacgcttt tgcacgcgaa
     2101 cggacatcac atgcttgtcg tatcggagac tttggcagaa gcccgcacgc gtatccggca
     2161 aaaaggcgcg ggcgaaatca cgtcgatcaa acggctctag ccgattcgct ttccaacgtc
     2221 tggcgatcga aagcctcagg caaaagggca gcgaatttga cgctcttatg caccccgtcc
     2281 ggcccggcac tgtgaacgac ggcgtcttcc gtcgcgaact cggcaatgcg ctggcgacag
     2341 gagccgcaag gcagaattcc acgatcgcaa tccgccacga caagaacatc ggagatttgc
     2401 cggccacccg cggcgaccat cgcggtgatc gccccggtct cggcgcagca tcccgccgga
     2461 taggcagcgt tttcgacatt gccgccggca aagatctgtc cgtccggcgt ggcgatcgcc
     2521 gcgccgacag cgaagtgcga ataaggcgcg taggcgttct cgcgcgctgt aagggcggca
     2581 gcaaagagct tgtcgagcgc cgcttgcgag attttatttt cggaaatcac gttcatgagc
     2641 tttgccggga tggagccacg gcagacatca tactagggtc ggccgcaacc ggaaactaat
     2701 tctcatccgc aaacacgccg ccacgttgct cgggcgggat gttgtacagt gggatgaccc
     2761 gaatatggtg atggaccggc gcctcgacaa ctacagtttt ctgcacaatg ggcatgcgcg
     2821 cctctgcgac cgcctcgttc ggcgaaatcc gcagagctgc catcgaaatc aggcaaaacc
     2881 ccaatgccaa agcggcgatg acgcgctgct gccggcgttc gcgccgaagc accggattgt
     2941 catgctgcgg atggcggcgg ctgggataaa gagcttcgat cgacatggtc tcaacctttc
     3001 atgcacgcct tcctaacccc atctatgctg cgcagttccg tgcgctttcg caccagccgc
     3061 gtcgatatag gttcgatgcg gcaagcgaca ggcacgctcg actccgttag agtgtggtct
     3121 tgtgaaggat tggaacagga catggacggg caagccgccg cacgggtgag cgtggagatc
     3181 aattttccca atggcggggt cttcggccgc gaggaaatcc tgctgatcaa cgcgatccgc
     3241 gcacagcgct cgatcatcgg cgcaagtcgt gcgctcggcc tctcctatcg caaatgctgg
     3301 ctggtcgtcg acgcgctgaa ccggacgttt gaaagtacgg tcattgcgac ctatcccggc
     3361 cggcgcggca gcggtgccga aatcaccgcc ttcggcgagc ggctcgtcgc gctctaccag
     3421 tcaatcgagc gtcacgccgg gacttcggcc gagagatcgc tcgcggaagt tacggcgtct
     3481 ctcgactgga atttcgatcc gaaggccagg agcgcggtcg cggggcgcgg tctggcctga
     3541 cgccacggcc ggaaatggcc accgccttga tcatcgccca ggctttgagt cccggcacca
     3601 aagccagtcg gccgaccgat tcgcgggtga tcagcgaatg cagccgcgtc ttgcccaggc
     3661 tgaacgtggc gcggacatag ggcggtgcca gaaattcaat ctcgtcgatc gcaccgggca
     3721 gacggttggt gatcgaaaca tccatcggcc gagacagcgc gatcgagaca tcgcgcgcat
     3781 cgatcttcac cacgacgctg ctgccgaccg gcagatcgac catcgggagg cgcaactcac
     3841 cgtcatcgaa gagcaggctc gtcagcgcca ggtcggggtc gtggctcagg atacgggcga
     3901 acaccgtcgt ttcgagcagc ggcggtccgc tcg
//
LOCUS       DASWOG010000415         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_357409,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000415 DASWOG010000000
VERSION     DASWOG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357409"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..188
                     /locus_tag="VGB93_08595"
     tRNA            115..188
                     /locus_tag="VGB93_08595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:147..149,aa:Cys,seq:gca)
     gene            complement(226..1017)
                     /locus_tag="VGB93_08600"
     CDS             complement(226..1017)
                     /locus_tag="VGB93_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146196.1"
                     /translation="MSASRYRAKPGDGIAFVSGASSGVGRACVLELARRGYVVAATAR
                     RAEELQALAAMSPEQIFPYPGDVTDDAGMKTLLAQIERDRGPIALAFLNAGVSLHIER
                     EGFDAAVVAKTYAVNIGGVANCLDPLLIAMSQRHRGQIALNASLAGYNGLPTSAAYGS
                     SKAALIYMGEALNLRYRSAGLTIQVVNHGFVRTAMTDQEKDFSQPFKIGPDEAAKLIC
                     DGFERGGFEIAFPWQLAILQKLVRALPYAAKFNFIDYGMKRAKQS"
     gene            1122..1875
                     /locus_tag="VGB93_08605"
                     /pseudo
     CDS             1122..1875
                     /locus_tag="VGB93_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563205.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
BASE COUNT          402 a          558 c          556 g          359 t
ORIGIN      
        1 cggcgcttgc aaagctcgaa tgaggctcgg ggacggtttt ccgcgaggct gagttgccgt
       61 ccgcggctcg ccttgctata gcgacgcacc gtggatgggg gaacctgtcg cgacggcctc
      121 gtggcggagt ggttacgcag aggactgcaa atccttctat cccggttcga ttccgggcga
      181 ggcctccaat ctggcggcgc cggtgcgacg gcaacgtgcg tccactcaac tctgcttggc
      241 gcgcttcatt ccgtaatcga taaaattgaa tttggcggca tagggcaaag cccgcacaag
      301 cttttgcagg atcgccaatt gccagggaaa ggcaatctcg aagccacccc gctcaaatcc
      361 gtcacagatg agcttcgccg cctcgtccgg cccgatcttg aatggctgac tgaaatcctt
      421 ttcctgatcc gtcatcgcgg tgcgaacgaa tccgtgattg acaacctgga tggtgaggcc
      481 cgcggagcgg tatctcaggt ttagcgcctc ccccatgtaa atcagcgctg ccttggagga
      541 gccataagcc gcggacgttg ggaggccatt atagcccgcc agcgaagcgt tgagggcgat
      601 ctggccgcgg tgcctttgcg acatcgcgat gagcagcgga tcgaggcagt tcgcgacgcc
      661 gccgatattg accgcgtaag tcttcgcgac aaccgccgcg tcaaaacctt cgcgctcgat
      721 atgcagcgaa acaccggcgt tgagaaatgc aagtgcgatc gggccgcgat ccctttcgat
      781 ctgtgccagc agagttttca tgccggcgtc gtcggttaca tcgcccggat agggaaaaat
      841 ttgctccggc gacatggcgg cgagcgcttg caattcttcg gcgcgccgag cggtcgccgc
      901 gacaacgtag ccgcgccgcg ccaattccag gacgcacgca cggccaacgc ctgagctggc
      961 accgctgaca aaggcaattc catcaccagg cttcgcgcga tacctgctgg cgctcatatt
     1021 cttttccgac cttaaggtga tggcgacacg ccggcgcggc ggctcgacag cccgcgtcgc
     1081 gagcctttat atagcccacg aatttactca ggaatggaaa tatgccgctc cggaaaaaca
     1141 ttatcgaggc tatcggtcac acgccgctga tcaaattgca gcacgcctcg atggccaccg
     1201 gctgcgagat ttatggcaaa gctgagttta tgaatccagg cggctccatc aaggaccgcg
     1261 ccgcgctggc aatcgtccgt gacgccgagg cgcgcggcac attgaaaaaa ggcggcatga
     1321 ttgtcgaggg aacggccggc aacaccggca ttggcctcgc ccttgtcgct aatgcgcttg
     1381 gctaccgcac ggtgatcgtg atcccggaca cgcagagcca ggaaaagaag gacttgctgc
     1441 gtttgcaggg tgcggaactt atcgaagttc cagccgtccc ctattccgat ccgaacaatt
     1501 acgtgaagct ttccggccgc ctcgcggaaa agctcgtgca tgagcatgta cagggcgcaa
     1561 tctgggccaa tcaattcgac aatgtggcaa accggcaagg ccatatcgaa acaactgggc
     1621 cggaaatctg gaccgatact gatggcaaga tcgatggctt tgtctgctcc gtcggaactg
     1681 gcggcacgct tgccggtgtc gcgatcgcgc tcaaggccag aaatccgaag atcaagatag
     1741 cgctggccga tccgctgggc gccgcgctct atgaatatta cacccacggt gttctgaaat
     1801 ccgaaggctc gtaaatcaca gagggcagcg gtcagggacg tatcaaagcc aatctcgaag
     1861 atgcgcccgt cgacc
//
LOCUS       DASWOG010000416         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_247946,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000416 DASWOG010000000
VERSION     DASWOG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247946"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..767
                     /locus_tag="VGB93_08610"
     CDS             291..767
                     /locus_tag="VGB93_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146197.1"
                     /translation="MKTTRSRRNIEDCKAPIAAPLPILRNAAIRVCGLGAALALIFAG
                     GICAMCPLAQAAPTPMPPQRPSSLAPEQSTPALPEQTSPQPDAPMDTAPATPPHAADE
                     PIKPGLINEGPGTLLKLPSASHARMHECAVEWQNMKTTGTAVEKTWFNFALKCLTR"
     gene            complement(946..1233)
                     /locus_tag="VGB93_08615"
     CDS             complement(946..1233)
                     /locus_tag="VGB93_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like protein 2"
                     /protein_id="HEY0146198.1"
                     /translation="MVKATTKRKPAKKAAKKTAKKAVKKAVKKTVRKAVGKKTVKKAT
                     RKVAKRATKTAAKKARTSARKSPVKKAAAKKARKSSPRKPKAPAPAAPESV"
     gene            complement(1871..3358)
                     /gene="gatB"
                     /locus_tag="VGB93_08620"
     CDS             complement(1871..3358)
                     /gene="gatB"
                     /locus_tag="VGB93_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0146199.1"
                     /translation="MNTHAAPSKLIKGATGDWEIVIGLAVHAQVVSKSKLFSGASTEF
                     GGEPNSHVSLVDAAMPGMLPVINSECIAQAIRSGLGLKAKINLRSSFDRKNYFYPDLP
                     QGYQISQYKSPIVGEGIVTVDVTPAERIEVGIERLHLEQDAGKSLHDQSPTDSFVDLN
                     RSGVALMEIVSKPDMRSADEAKAYVTKLRTILRYLGTCDGNMEQGSLRADVNVSVRRP
                     GEGLGTRCEIKNVNSIRFIGQAIEYEARRQIGILEDDGKIDQETRLFDPTKGETRSMR
                     SKEEAHDYRYFPDPDLLPLEFDQTYVDDLAAHLPELPDAKRARFIADYGLPAYDATVL
                     VADKETADYYEAAIAHGGAKRDAKVVANWVMGDVSAYANSVGLAVPQTHLTPAQIAGL
                     VDLIADGTISGKIAKDVLLILIEDEKNGDPRAIVEARGMKQVTDLGAIETAVDQIIAA
                     NPDKVAQALAKPSMLGWFVGQVMKQTGGKANPQAVNELLKTKLGI"
     gene            complement(3402..>4003)
                     /locus_tag="VGB93_08625"
     CDS             complement(3402..>4003)
                     /locus_tag="VGB93_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0146200.1"
                     /translation="GAKVVEISLPHTRYALPAYYIVAPAEASSNLARYDGVRYGLRVP
                     GKDITDLYENTRAAGFGKEVRRRIMIGTYVLSAGYYDAYYVRAQKIRTLIKRDFETAF
                     AAGVDAILTPATPSAAFGQGEKGSADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSSEG
                     LPLALQLIGRAFDEETLFSVAAAIEQAAPKIDLPPKWWA"
BASE COUNT          805 a         1205 c         1163 g          830 t
ORIGIN      
        1 tgacggctgg ccagccttaa cgacgtcagc cgatcattaa tcaatccgtg ggtggcgtta
       61 tgcgtaaatt ggtggggcta ttcaaggcgg ataggcttaa acttcatcgc aagcgcgtga
      121 tcggctttaa agatttcgtt agcttgcatc tgagccctat ccggtcccgc gtccggtgca
      181 gagcagcacc gcgagaattt ctcagcggcg ccacgatccg accctagaca cgcctatgcc
      241 gcgcggctta accgagcccc gaccccacgc cccaggacct gctggcagga atgaaaacaa
      301 cccgaagccg acgcaacatt gaagactgca aagccccgat cgccgcgccg ctgccgatcc
      361 tgcgcaatgc cgcgatacgt gtctgcgggc ttggtgccgc gctggctttg atcttcgcgg
      421 gaggtatctg cgcaatgtgc ccattggcgc aggcggcgcc tacgccaatg ccgccgcagc
      481 ggccgtcgag tctcgcgccg gaacagagca cgcccgcgct gccggaacaa acatcacctc
      541 agcctgacgc cccaatggac acggctccgg ctacgccgcc gcacgcagcg gacgaaccga
      601 tcaaacccgg attgatcaat gaagggccag gaactctgct gaaactgccg tcagcgtcgc
      661 acgcccggat gcatgagtgc gcagtcgaat ggcagaacat gaaaacgacc ggcacggcgg
      721 tcgagaagac gtggtttaac ttcgcgctta aatgcctgac gcgataaacc acacgcgaca
      781 tagccgccga tcgggcctgc cgggacgcag tgtaatagag caaatcaacg aagacggaac
      841 ggccagtgtg gcatcagagc caaatggggc tcagagcaca tcctcgggcg aaaaccgcga
      901 accgaggaaa aatctggccg tctccgtctt cttgtttcaa cgagtttaga ccgactccgg
      961 cgccgccggc gctggggcct ttggcttgcg cggggaagac ttgcgcgcct ttttggcggc
     1021 agccttcttc accggcgact tgcgggccga tgtacgggcc ttcttcgccg cggtcttcgt
     1081 ggcgcgcttc gcaactttgc gcgtcgcctt ctttacggtc ttcttgccaa ccgctttgcg
     1141 gaccgtcttc ttcacggcct tcttcacggc cttcttcgca gttttcttcg ccgctttctt
     1201 agcgggttta cgtttggtgg tagccttcac catcttcaag ttccctctgt tgttttgccg
     1261 gcatgcgttt ctttgccgac aagaggcgta aatagcgtag cgcggttaat cagtcgtgca
     1321 agaggggctt gcgtacgaga ggccgaatcg ttcagcgatt ttgcgccctt tgtcttagta
     1381 agacgttgct tcatgcgttg attgcgaagc cttcggcaac atcaacgcgt ggcattcgca
     1441 ggccctgcat cagcgcacac gaacagcgtg caaagatgcg caacaaaatt ccagacaaca
     1501 ccgcattcga ccgcacgcga ctgcgttgct catgcgcacg cgaatgcaaa gttattgttt
     1561 ttcttgaatc aatttccgtt tcgtgtcttc agacgtgaac gatgatgaag atcgtttgcg
     1621 cgtcttcgca cgcgcgtcgc acccacagtg aaaggcgctt tgtcaacgtg cggcgacgtc
     1681 tcaccgtcga cgcaacacgc ttcgacgcgg cgctgtgggg acccgcagac gattgccatc
     1741 gcagggttaa aacgtcggca aaaatttttt tgtgggactc gaaaaaaaat gcgcaaaagt
     1801 gcttcgcgca aggtttcgca aagcctcgaa acgcgattcg cacgattcgt ggactgattc
     1861 gaaccggtgt tcagatgccg agtttggtct tcagaagctc gttaacagcc tgaggattgg
     1921 ccttaccgcc tgtttgcttc atcacctgtc cgacgaacca tccgagcatt gatggcttgg
     1981 ccagagcttg cgcgaccttg tcgggattgg cggcgatgat ctgatcgaca gccgtttcga
     2041 tggcaccgag atcggtgacc tgcttcattc ctcgcgcttc gacgatggca cgcggatcgc
     2101 cgttcttctc gtcctcgatc aggatcagca gcacgtcttt ggcgatcttc ccggagatcg
     2161 tgccgtcggc gatgagatcg acgagcccgg cgatctgggc gggcgtcaga tgcgtctgcg
     2221 gcacggcgag gccaaccgaa ttggcgtaag ccgacacatc acccatgacc caattggcaa
     2281 cgaccttcgc atcgcgcttg gcgccaccat gcgcaatggc ggcctcgtaa tagtccgccg
     2341 tctccttgtc ggcgacgaga acagtcgcgt cgtaagccgg cagaccataa tcggcaatga
     2401 aacgcgcgcg cttcgcatct ggcaactccg gcagatgcgc cgcgagatcg tcgacatagg
     2461 tttgatcgaa ttcgagcggc aaaagatcgg ggtcggggaa atagcggtag tcgtgcgcct
     2521 cttcctttga gcgcatcgag cgcgtctcgc ccttggtggg atcgaagagc cgcgtttcct
     2581 gatcgatctt gccgtcgtct tcgagaatac cgatctgccg gcgcgcctca tattcgatcg
     2641 cctggccgat gaagcggatc gaattgacgt tcttgatctc gcagcgtgtg ccaagaccct
     2701 cgcccggccg ccggacggaa acattgacgt cggcgcgtag cgaaccctgt tccatattgc
     2761 cgtcgcaggt gccgaggtag cgaaggatgg tcctcagttt ggtgacgtag gccttcgcct
     2821 catcagcaga ccgcatatcc ggcttggaga caatctccat cagcgcgaca ccggagcgat
     2881 tgagatcgac gaaggagtcc gtcggcgatt ggtcgtgaag cgatttgccg gcgtcctgtt
     2941 ccagatgcag ccgctcaatc ccgacttcga tccgctcggc cggcgtcacg tcaaccgtga
     3001 cgatcccctc gccaacgatc gggcttttgt actgcgagat ctgatagccc tgtggcagat
     3061 cgggatagaa ataattcttc ctgtcgaagc tcgagcgaag gttgatcttg gcctttagac
     3121 cgagaccgct gcgaatcgcc tgggcaatgc attccgaatt gatgaccggc aacatgccgg
     3181 gcatggccgc atcgaccaac gagacatggc tgttcggctc gccgccgaat tcggtcgaag
     3241 caccggaaaa gagcttcgac ttcgagacca cctgggcatg aaccgccagg ccgatcacga
     3301 tttcccaatc gccggtcgcg cctttgatga gcttcgaggg ggctgcatgc gtgttcatgg
     3361 gatgcctcag ttcatttcta tcttggtatg ccgtccgcag atcacgccca ccatttcggc
     3421 ggcaggtcga tcttcggcgc ggcttgctcg atagccgcgg caacagagaa taatgtctct
     3481 tcgtcgaagg cgcggccaat caattgcagc gcaagcggta ggccctccga tgaaagcccc
     3541 gcaggcaccg cgatgccggg cagaccggcc atgttaaccg tcacggtgaa gacgtcgttc
     3601 aaatacattt cgaccggatc ggcgctgccc ttctcgcctt ggccaaaggc cgccgacggt
     3661 gtcgccggcg tcaggatcgc atcgacgccc gcagcgaaag cggtctcgaa atcgcgcttg
     3721 ataagcgtgc ggattttttg cgcccgcaca taataagcat cgtaatagcc ggccgacaga
     3781 acataggtgc cgatcatgat gcgccggcgc acttccttgc cgaaaccggc cgcgcgcgtg
     3841 ttttcgtaga gatcggtaat atccttgccc ggcacgcgca gcccatagcg cacgccgtca
     3901 tagcgggcga gattggacga ggcctcggcc ggcgccacaa tataataggc gggcagcgca
     3961 tagcgcgtgt gcggcagcga aatctcgacg accttggcgc cgg
//
LOCUS       DASWOG010000417         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_28097,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000417 DASWOG010000000
VERSION     DASWOG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28097"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..911
                     /gene="era"
                     /locus_tag="VGB93_08630"
     CDS             45..911
                     /gene="era"
                     /locus_tag="VGB93_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0146201.1"
                     /translation="MGAPNAGKSTLLNQLVGAKISIVSRKVQTTRTLVRGIALQGEAQ
                     IIFVDTPGIFAPRRRLDRAMVASAWGGASDADVVALLIDARKGIDDEVQEILRALEGV
                     KSKRVLILNKIDLVARPSLLELAAAVNEKLAFAETFMISATNGDGVETLKNHLAAMMP
                     AGPWLYPEDQISEAPIRALAAEITREKIFERLHDELPYQSTVETESWEEKPDGSARVE
                     QTIFVTREGHRKIVLGEGGRTIKAIGVAARKDIAEAAEQKIHLFLFVKVRENWADDPE
                     HYRTMGLEFPKN"
     gene            1314..>2752
                     /locus_tag="VGB93_08635"
     CDS             1314..>2752
                     /locus_tag="VGB93_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0146202.1"
                     /translation="MSRRRAFSQGRSQPRTVLWFAILLALPLAVFGIFAFYLFNADYA
                     VSHTREASQAAQKAAELGDVAGSALEAKADHAIAQVNAALAKGGTEMMRNLRVASFVN
                     EVRFLLVYDGDSKVIPLATGTLETENDFLRHFSGLFSTLRGQIDSAGGAPNVGVWTVG
                     EELVSYVRCQHTSGIRDICVLFDEITLDPLFEETLDKASAKLNDRLLILRDPVGRALW
                     SHGPSNLSTSAISDLSGSMRGWRMETSTPAVAHHGLLPLIAITVPLIGCWLLLIWYTY
                     RNQQARLVESTFRSEMTAKLSHDLRTPIANLRLYTDLLLRRSGDEMAVNRYGTVMRAE
                     VERLALLADNTIVYGRTAAPVPVRLEAAVPDAIVDATIARYENLFAAGGSMIEVTHNA
                     PRAGLFDRMSFERILINLIDNANKYAPGRILVTTDETDGTLTLSVRDYGQGLDPKLQA
                     KLTALVQPGGGAKDGFGLGLVVVRELAET"
BASE COUNT          544 a          820 c          848 g          540 t
ORIGIN      
        1 agcccgggca tgagcagcag cggcgctgcg gttttgttgc actcatcggt gcgcccaacg
       61 ccggcaaatc gacactgctt aatcagcttg tcggcgccaa aatctccatc gtctcgcgca
      121 aggtgcagac gacgcgcacg ctagtacgcg gcattgcctt gcaaggcgaa gcgcagatca
      181 tcttcgtcga tacgccgggt attttcgcgc cgcgccggcg gctcgatcgg gcgatggtcg
      241 cgtcggcgtg gggcggcgcc agcgacgcgg atgttgtggc gctgctcatt gatgcccgca
      301 aaggcataga cgacgaagta caagagattt tacgcgcgct cgaaggcgtg aaatccaagc
      361 gcgtgctgat cttgaacaaa atcgaccttg tcgcccgacc gagcctgctc gaactcgcgg
      421 cggcggtgaa cgagaagctt gcgttcgccg agacgtttat gatctcggcg accaatggcg
      481 acggggtgga gacgctgaaa aatcatctcg cggccatgat gcccgctggg ccgtggctct
      541 atcccgagga ccagatttcg gaggcgccga ttcgcgcgct tgccgccgag atcacgcgcg
      601 aaaagatctt cgagcgtctg cacgacgaat tgccatatca atcgacggtc gagacggaat
      661 cctgggaaga aaagccggac ggctcggcgc gggtcgagca gacgatcttc gtgacccgtg
      721 agggccaccg caagatcgtg ctgggcgagg gcggccggac gatcaaggcg attggcgttg
      781 cggcgcgcaa agacatcgcc gaagcggcgg agcagaagat ccacctcttc ctgttcgtca
      841 aagtgcgcga gaattgggcc gacgaccccg agcactaccg gacgatgggg ctggaatttc
      901 ctaaaaatta gagcgtcgaa ctgaagggaa aagaaaaagc cggcccttgg gccggctttt
      961 gcgcgatgtc gtgattgcga gggaggattc gcctcgcgac taggaaatca aaactcgccg
     1021 atttcgctcg actcgtaagc ggtgacttcc atgccgacgc aaacttcaca agcaacgggg
     1081 gttgtccaag ccattttttc tctcctgttt taccaggccc cctttggagg cctcaaaacc
     1141 ctgttctcgc aacatagggc gataaccggc gggattggtc agacattgga aagatcgatg
     1201 taagaatttt gtcagcaacg ccgaggcttc cctgctgcgg cgcagcatgg caaagctccg
     1261 gctggcgtcg cggcccgcgg agaacatgct aaagattcgg catgagcatc gagatgtcca
     1321 gacgtcgcgc cttcagccag ggccgctcgc agccgcgcac ggtgctgtgg tttgcgattc
     1381 tgctggctct gccgctggcg gtcttcggca ttttcgcctt ttacctcttc aatgccgatt
     1441 atgcggtgtc gcacacccgt gaggcgtcgc aggcggctca gaaagcggcg gagcttggcg
     1501 atgtcgccgg ttcggcgctt gaggccaagg ccgatcacgc catcgcgcag gtcaatgctg
     1561 cgctcgccaa aggcgggacc gagatgatgc gcaatctgcg cgtcgcgagc ttcgtcaacg
     1621 aagtgcgttt cctgcttgtc tatgacggcg acagcaaagt catcccgctg gcgacgggga
     1681 cgctcgaaac ggagaacgac tttctccgtc atttcagcgg cctcttctct acgctgcgcg
     1741 ggcagatcga tagtgcgggc ggcgcaccga acgtcggggt gtggacggtg ggcgaggaac
     1801 tggtctctta tgtccgttgc cagcatacca gcggtatccg cgacatctgc gtgctcttcg
     1861 acgaaatcac tctcgatccg ctgtttgaag aaacgctcga caaagcaagc gccaagctta
     1921 acgaccggct actcatcctg cgcgatcccg tcggccgtgc gctctggagc cacgggccat
     1981 ccaatctcag cacgtcggcg atctccgacc tttcaggctc gatgcgcggc tggcggatgg
     2041 aaacctcgac accggccgtc gcgcatcatg gtctgttgcc gttgatcgcc attacggttc
     2101 ccttgatcgg ctgctggctg ctgctgatct ggtacacgta ccgtaatcag caggcgcgcc
     2161 ttgttgaaag taccttccgc agtgaaatga ctgcaaagct ttcgcacgat ctgcgcacgc
     2221 cgatcgctaa tctgcggctt tatacggatc tgcttctgcg ccggagcggc gatgaaatgg
     2281 ccgtcaatcg ctatggcacg gtgatgcgcg ccgaggtcga acgccttgcg cttttggccg
     2341 acaatacgat cgtctacggt cgcaccgccg cgccggtgcc cgtccggctt gaagcagcgg
     2401 tgccggatgc aatcgtcgac gcgaccatcg cgcgctacga aaatctcttc gccgccggcg
     2461 gctccatgat cgaggtcacg cacaacgcgc cacgcgccgg acttttcgat cgcatgagct
     2521 ttgagcgcat tctcatcaac cttatcgaca acgccaataa atacgcgccg ggacgtattc
     2581 tcgtcacgac cgatgagact gatggcacgc tcacgctcag cgtgcgcgat tacgggcagg
     2641 gccttgatcc caaactgcaa gcgaaattga cggcgcttgt gcagccgggc gggggcgcga
     2701 aagatggctt cggcctcggc cttgtcgtcg ttcgcgaatt ggccgagacc aa
//
LOCUS       DASWOG010000418         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_266739,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000418 DASWOG010000000
VERSION     DASWOG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266739"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGB93_08640"
     CDS             <1..208
                     /locus_tag="VGB93_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146203.1"
                     /translation="VLIVGGIILVVMLGVSPGFRWFFFNVLLSALLSSGGSRGGGDSW
                     GGSSGGGFSGGGGSSGGGGASGGW"
     gene            208..816
                     /locus_tag="VGB93_08645"
     CDS             208..816
                     /locus_tag="VGB93_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0146204.1"
                     /translation="MISANDRTRISAAIHASEAKTSGEIVCVLAESSSQPGALPVLIA
                     AIVALALPWLLITFTNFAVERILLLQILVFVLLTVLLLLPPVYLALLPQRVKRAAAYR
                     LATEQFVVRGIARKKDRSGILIFVSLAERYARIIADEGVAARTSQAEWQAAIDVLVSH
                     MRQDQIADGFIAAIGACSDVLAVHFPHIEAGQDELPDRIYVI"
     gene            941..2323
                     /locus_tag="VGB93_08650"
     CDS             941..2323
                     /locus_tag="VGB93_08650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0146205.1"
                     /translation="MTETAVAGPLTDSPIAPLGPSDQANYNVVIVGGGASGLELATQL
                     GDRFRRTKAGRVTLIDRSRTHLWKPLLHSVAAGSMDPSEHELDYLAQAYWHNFRYRFG
                     DMVGIDRERKLVQMAPTYDEENRLITPARSIPYDTLVIAVGSITNDFGTPGVAEFAVP
                     LETAEQAKRFNRRLINACLRAHTQSEPIRPGQLHVAIVGAGATGTELAAELYRTVREV
                     VAFGMDRIDPAVDVRIILLEAGPRILPALPEALSASTMETLRKLGVEVRTNARVSAVQ
                     ADGIHLADGQFIPSELVVWAAGVKAPDVLQNIGGLETNRANQLIVNRTLQTTRDPNIF
                     AMGDCAACPLDDSGSGATVPPRAQAAHQEASHLYKQIQNQMQGKPIEPYRYRDFGSLV
                     SLGKYSTIGNLMGAIIGPNFRVEGWFALLMYRSLYKMHEYALHGGRRVILSILSRGLS
                     HRAEPEVKLH"
     gene            complement(2356..>3359)
                     /locus_tag="VGB93_08655"
     CDS             complement(2356..>3359)
                     /locus_tag="VGB93_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0146206.1"
                     /translation="NRYVNRTLVQRSRCGHQGCTFCAEPLMNGLKFRYRPVDEVIREM
                     ENCGERNISINDADFFGTPERPKELMRAMKGRGLQWQAGVTSKLAQDDEMLELAAESG
                     CTLLSIGFESISRSTLTSVHKHVNRPDQFAALVEKVHSYGIMVFGLFMFGFDGDDLDV
                     FDETVKFNIEADYDACAYSVLTPYPGTLTWYELRKANRIVSYDWTMYDQGHVTYLPAQ
                     MEPDELRVGHARAYENFYKLSSMARRFPWDKRKRAQWSVYNAFMRKAAKTDHIDSIAP
                     PTADPTVAPMPPLLPIKKEWRAAVLEAAGAPDPETAHLAPAPISCDVGMDRPAAAAE"
BASE COUNT          575 a         1033 c         1050 g          701 t
ORIGIN      
        1 ggtgctgatc gtgggtggta tcatcctggt tgtgatgctc ggcgtttcgc ccggcttccg
       61 ctggtttttc ttcaatgtcc tgttgagtgc gctgttaagc tcgggcggct cgcgcggcgg
      121 tggcgattca tggggcgggt cctcgggtgg gggcttttcc ggcggcggcg gctcgtcggg
      181 cggcggcggc gcctcggggg gttggtgatg atctcggcaa atgaccgcac ccgcatttca
      241 gcggcgatcc acgcgtcgga ggccaagacc tcgggcgaaa tcgtttgtgt tctcgccgaa
      301 agctcatccc agcctggcgc gctgccggtt ctgatcgcgg cgatcgtcgc attggctctt
      361 ccctggctgc ttatcacttt cacgaatttt gccgtcgagc gaattctgct gctgcaaatc
      421 cttgtttttg tgcttctgac ggtcctgctt ctcttgccgc cggtctatct cgcactcctg
      481 cctcagcggg taaaacgggc cgccgcgtac cggctggcga ccgaacaatt tgtcgttcgt
      541 ggcatcgcac gcaaaaagga tcgcagcggc attttgattt tcgtctcgct ggcggagcgc
      601 tatgcgcgca tcatcgccga tgagggtgtc gctgcccgca cgtcgcaggc cgaatggcag
      661 gcggcaatcg acgtgctcgt ctcgcacatg cgtcaggatc agattgccga cggtttcatc
      721 gccgcgatcg gcgcctgcag cgatgtgctg gcggtgcatt tcccgcacat cgaggcgggg
      781 caggacgaat tgcccgaccg gatttacgtg atctaggcgc cgccaccact agacaggcct
      841 ttccctgggt ggaatctggc cgttggaata accatcttta gcttcacggc ggcctcgctg
      901 cgaagcttga gaccttgccc gcttgttccc ggagctatca atgaccgaaa ctgccgtcgc
      961 cggcccgctc actgactcgc ccatcgcgcc gctcggcccg agcgatcagg cgaattataa
     1021 tgtcgtcatc gtcggcggcg gcgcttcagg gcttgagctt gcgacccagc tcggcgaccg
     1081 tttccggcgc accaaggccg gccgcgtgac gctgatcgat cgctcgcgca cgcatctgtg
     1141 gaagccgctg ctgcactcgg tcgcggccgg cagcatggac ccgagcgagc acgagctgga
     1201 ttatctggcg caggcctatt ggcataattt tcgttatcgc ttcggtgaca tggtgggcat
     1261 cgaccgcgag cgcaaactcg tccaaatggc gccgacctac gacgaggaaa accgcctgat
     1321 cacaccggcg cgcagcattc cctacgacac gctcgtcatt gccgtcggca gcatcaccaa
     1381 cgatttcggc acgcccggcg tcgctgaatt cgccgtgccg cttgagacgg ccgagcaggc
     1441 aaagcgcttc aaccggcgcc tcatcaacgc ctgtctgcgc gcccacacgc aatctgagcc
     1501 aatccggcct ggtcagcttc atgtcgcgat cgtgggtgcc ggcgcgaccg gcaccgagct
     1561 tgccgcagag ctttatcgca cggtgcgcga ggtcgtggcg ttcggcatgg accgcatcga
     1621 tccggccgtc gatgtccgca tcattctgct cgaagcaggt ccgcgcattc tgccggcgtt
     1681 gccggaagcg ctttcggcct cgacgatgga aacattacgc aagcttggcg tcgaggtgcg
     1741 gacaaatgcg cgcgtctctg ccgttcaggc cgacggcatc cacctggccg atggccaatt
     1801 tattccgtcg gaacttgtgg tctgggcggc gggcgtaaag gcgcccgacg ttctgcaaaa
     1861 catcggcggt ctggagacga accgggccaa ccagctgatc gtgaaccgca cgctgcagac
     1921 gacgcgggac ccgaacattt tcgcgatggg tgattgcgct gcctgtccgc tcgacgacag
     1981 cggcagcggc gcgacggtgc cgccccgcgc tcaggcggcg catcaggaag cgagccatct
     2041 ctacaagcag attcagaatc agatgcaggg caagccgatc gaaccctatc gttatcgcga
     2101 tttcggctcg ctggtgtcgc tcggcaaata ttcgacgatc ggcaatctga tgggcgccat
     2161 catcgggccg aacttccgcg tcgaaggatg gttcgcgctg ctgatgtatc gctcgctcta
     2221 caagatgcac gaatatgcgt tgcacggggg gcggcgcgtc attctcagca tcctgtcgcg
     2281 cggtctctcg caccgcgccg agcctgaggt gaagctgcat taaaaaaggc gccgggtgat
     2341 cccggcgccc ttcaattatt cggccgcggc ggccggcctg tccatgccga catcgcagct
     2401 tattggcgcc ggcgcgagat gcgcggtttc cggatcgggc gccccggctg cttccagcac
     2461 ggcggcgcgc cattccttct tgatcggcaa aagcggcggc atcggcgcga cggtcgggtc
     2521 ggcggtgggc ggggcgatcg aatcgatatg atcggtcttg gcggccttgc gcatgaacgc
     2581 gttataaaca ctccattgcg cgcgcttgcg tttgtcccac ggaaaacgcc tggccatcga
     2641 cgacagcttg tagaaatttt cgtaggcgcg cgcgtggccg acacgtagtt catccggctc
     2701 catttgcgcc ggcagatagg tgacatggcc ctgatcgtac atcgtccagt cataggagac
     2761 gatgcgattg gccttgcgta gttcgtacca agtcaatgtg ccggggtagg gcgtcagcac
     2821 cgaataggcg caggcgtcat aatcggcttc gatgttgaat ttcaccgttt cgtcgaaaac
     2881 gtcgagatcg tcgccatcga agccgaacat gaacagaccg aagaccatga tgccgtagga
     2941 atgcactttc tcgacaagtg cggcgaactg atccggccga ttgacgtgct tgtgcacact
     3001 ggtcagcgtc gatcgcgaaa tcgattcgaa gccaatgctg aggagcgtac agccgctctc
     3061 tgcggcgagt tccagcatct catcgtcttg cgcgagtttg gacgtcacac cggcctgcca
     3121 ttggaggcca cggcctttca tggcgcgcat cagctctttc ggccgttccg gcgtgccgaa
     3181 aaagtcggcg tcgttgatcg agatgttgcg ttcgccgcaa ttttccatct cgcggatgac
     3241 ttcatcgacc ggccggtaac ggaatttcaa cccgttcatc agcggctcgg cgcagaatgt
     3301 gcagccctga tggccgcagc gcgacctctg cacgagggtg cggttgacgt aacggttct
//
LOCUS       DASWOG010000419         7717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_357624,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000419 DASWOG010000000
VERSION     DASWOG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357624"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2985)
                     /locus_tag="VGB93_08660"
     CDS             complement(<1..2985)
                     /locus_tag="VGB93_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146207.1"
                     /translation="MPRIAITSGRRHLLASSSIAALLIAAGTPAWAACAINQVGPAAS
                     VSNSSGSSINCINVQNSTVSGDVTNEVGGTITATGNTSPTTAGITINNSSIGGTVSNA
                     GTITATSGVGIIVENNSTISGGVRNSGTISVSGDLGNSQSGAISPLDELSSGILVGDL
                     ANGAPVAISTFAGGISNSGTISSSGSLGNGVLVGGFGNTQTSVTISAFSGGVSNSGTI
                     TASGVLSGGILVGGGALSESPVIISTFSGGISNSGTISMSQRDSSGIFVGGLENTQAS
                     VTISTFSGGITNSGTISISTSGSNGIVVGGFGISQAVAVTISAFSGGISNSGTITANG
                     HGILVGGTEAQGIAEPPVTISTFSGGINNSGTILGGIFVNDVESFFGGINNSGSISGN
                     NAIFVDDVKNFFGGINNSRTISAESDIAVLNVNTFSGGISNSGTIGGSDDGNGIFVGS
                     VSLFSGGISNSGTISLDGQFNNGISIDDVKSFSGGISNSGMISAVTGIAIAGSTITGG
                     IIDSGAIEATSHGITIDGQSQINATHTAISVIGPTFTGGITNAGTLSAENAGIGLDGV
                     SSFSGDISNSGTISAGTGIAVANTTINGEIIDSGTIVTTNHGIAIDSRSRINTTGTAA
                     IVVTGTTFTGGISNAGTISGGVNGILVQTANFLPSPTNSFSGGISNSGSISASTGAAI
                     SISSISAFSGGVTNSGTLSAGGISTLFGFAIGTGIAVGLVSSFSGNIRNGGLITAADI
                     GINLGNLGVFSGNIENTGTINSGGAGISVDGISTFSGGVFNTGTISAATQGIGIGAST
                     FTGGVSNGGAIIAGAADTGIAVTAATSFSGGVSNSGTISGGAGIALLFDSDFSGGISN
                     SGTISAGVNGIFVELISAFSGNISNSGTISAANAIAIEGGVTFASGAAIINSGTLTGT
                     ISAINAVDATSAVTIDQTGGLISGDIKLSASADVLNISGGTINGSIIGSGTSDTINFV
                     HAAGNTFTYASSFGFSTVNQVNV"
     gene            3350..7126
                     /locus_tag="VGB93_08665"
     CDS             3350..7126
                     /locus_tag="VGB93_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0146208.1"
                     /translation="MGQVQSGAAALVPLGEALAYIERCRGEGRLIEAEAVCRQILDAQ
                     PNLPEAWHLLGITAHQAGRLSDAIAHLTRATQLSPQNALFLSNLGEMYRQGGQLKRAI
                     ETGRRAVAADPAMAVAWSNLGAAYFELKDYKDAIEANQHAIAADPNLAQGHCNLGNAL
                     HGLKRFDEAIIAYRRAVEINPSYVDALANLGTALHHNGHYAEAMAALRRAIALSPEHA
                     NAHSGLGILLLMQGDFAEGWAEYEWRLRSSERKGPRFPVRGWQGESLVGKHIYVQAEQ
                     GFGDTLQFVRYIPWLAKRAGKVSLRVHQELLSLMRESFPGIDVYGDRGDPGPFDCDIA
                     LMSLPQFFKTRFETIPSEVPYLRASVATTERWAQRLAPMHGLKVGLVWGGNPEHVNDR
                     RRSLDIAHFGPLLDVPGVSFASLQFGPRVMDLKKLKGTSKVLDLSRELGDFANTAGAV
                     MALDVVITIDSSSAHLAGALGKPVWTLISDIADWRWRLNIEDNVWYPNMRLFRQGPGE
                     DWSHVVARIETALRLAGAGDTSALLPFWELGAGRGADARTILEIETQRARIPSAVAAP
                     ERPTSPGHLFILAERKRRAGFLGDAEEFSRRAIESQPGNAEAIHNLGLTAHQSGKAHE
                     AIAHLRRAIALAPDQALYHANLGEMCRLAGLTDEAISEGRRAVEIDPNYPSALSNLGI
                     ALFDQNKFEDALDYLDRAVALQPDFAKAHSNRGNALLRLRRFAEAEPSYRRAVELEPD
                     FADGWNNLGTCLRELKHCEDAEVAYRKALALKPHDPDILDNLALALKDLSRLDEAAEC
                     ARRALAIEGRDPKLHIHAGVIFLDQHKIDEAGTAAAHALALNPNDHDVLNLMGRVAFE
                     REKLDESVDYYRRALAVKPDLADGHNNMGNALKEFGRLDEAREAYIRAIDLDPASTGA
                     YVNLADSMKFRPGDPILARMEALANKTESLSQIDRIHLDYALGKAYADLADHKRALTH
                     FIAGSAAKRQMISYDEAAAMTFFDRIENVFTADLMAEKSGHGDSSKRPIFILGMPRSG
                     TTLVEQILASHPMVHGAGELTEFNEAVDSIKGLDGAALTYPEFVPPLGASALRQLGGR
                     YLELVCKRSPSGERLTDKMPSNYYFLGLIHLALPNAKIIHTVRDPIDTCISCFSKLFS
                     ADQNHTYDLAELGRYYRRYEQLMTHWRRVLPTESFLDVQYEDVVANLEGAARRIIAYC
                     GLPWDDRCLAFHETNRPVRTASATQVRQPIYKNAIGRWRVYQEFLGPLLTALER"
BASE COUNT         1612 a         2227 c         2308 g         1570 t
ORIGIN      
        1 gacattcact tgattgacgg tcgaaaaacc gaagctactg gcataggtga acgtgttacc
       61 cgcagcgtgc acgaaattga tcgtgtcgct cgtccccgac ccgataatgc tgccattgat
      121 cgtgccgccg gaaatgttga gcacgtcggc actggccgag agttttatgt cgcccgagat
      181 cagaccgccg gtctgatcaa tcgtcactgc gctcgtcgca tccacggcat taatcgccga
      241 tatcgtgccg gtgagcgtcc cagaattgat aatggccgcg ccggacgcaa aggtcacgcc
      301 accttcgata gcgatagcat tagcagcgct gatcgtgccg gagttcgaga tattgcccga
      361 aaacgcagag atcaattcaa caaaaatacc gttgacgcca gccgaaatcg tgccggaatt
      421 tgaaatacca ccggagaaat cggaatcgaa gagaagcgct ataccggcgc caccggaaat
      481 ggttcccgag ttggaaacgc cgccggaaaa acttgtcgcc gccgtgacgg caataccggt
      541 gtcagcggcg cccgctatga tggcgccgcc attgctgacg ccgccggtga aggtgctggc
      601 gccgatcccg atgccctgcg ttgcggccga tattgtgccg gtgttgaaaa cgccgcctga
      661 aaacgtcgag atgccatcaa cgcttatgcc ggccccaccc gaattgatcg tgccggtatt
      721 ttcaatgttg ccggaaaaga cgccaaggtt gccgagattg atgccgatat ccgcagccgt
      781 gataaggccg ccattcctga tattcccgga aaagctgctg acaagaccga cggcgatacc
      841 ggtgccgata gcaaagccga aaagggtcga tattcctccc gcggaaagcg tgccggaatt
      901 tgtgacgccg ccggagaagg ccgatatcga tgaaatgcta atggccgctc cggtcgatgc
      961 cgatatggag ccagaattgg aaatgccgcc tgaaaagcta ttggtcggcg acggtaaaaa
     1021 gttggcggtt tggacaagga tgccgttgac gccgcccgaa atcgtgccgg cgttcgatat
     1081 gccgcctgtg aaggtcgtgc cggttacgac gatcgccgcg gtgccggtcg tgttgatccg
     1141 gctccgactg tcgatcgcaa tgccgtggtt cgtcgtcacg atcgtgcccg aatcgataat
     1201 ctcgccattg atcgtggtgt tggcgacggc aatgcccgtg ccggccgaaa tcgtgccgct
     1261 attgctgatg tcgccggaaa agctgctgac gccgtcgagg cctatgccgg cgttttcggc
     1321 cgagagcgtg ccagcgttgg tgatgccgcc ggtgaaggta gggccgatga cggaaatggc
     1381 cgtatgcgtc gcgttgatct ggctctgacc gtcgatcgtg atgccgtgac tggtcgcctc
     1441 gatcgcgccg gaatcgatga tgccgccggt gatcgtgctg ccggcgatgg cgatgcccgt
     1501 tacggccgag atcatgccgg aattgctgat gccgccggag aagcttttga cgtcgtcgat
     1561 tgagatgccg ttgttaaact gtccatccaa agaaatcgta ccggaattgc tgatcccgcc
     1621 ggagaaaagc gagacgctac cgacgaaaat gccgttgccg tcgtctgacc caccaatcgt
     1681 cccggaattg ctgatgccgc cggagaaagt gttgacgttg agaacagcta tgtcgctttc
     1741 ggccgaaatt gtgcgggaat tattgatacc gccgaagaag tttttgacgt catcgacgaa
     1801 gatcgcgttg ttccccgaga tcgagccaga attattgatg ccgccgaaga agctttcgac
     1861 atcattgacg aagatgcccc ccaggatcgt gcctgaatta ttgatcccgc cggagaaggt
     1921 tgagatcgtg actgggggct cggcgatgcc ttgcgcctca gtgccgccga cgaggatacc
     1981 gtgaccattc gcagtaatcg tgccggaatt gctgatgcca ccggagaagg ccgagatcgt
     2041 gacggcgacg gcttggctga taccaaaacc gccgaccacg ataccgttgc tgccagacgt
     2101 agaaatggaa atcgtaccgg aattggtgat tccgccggag aaggtggaaa tggtgactga
     2161 ggcctgggtg ttttccaagc cgccgacgaa aatgccgctg ctatcccttt gcgacataga
     2221 aatcgtgccg gaattgctga tcccgccgga gaaggtcgag atgatgacgg ggctttcgct
     2281 caaggcgccg ccgcctacga ggatcccgcc gcttagcact cccgacgcag taatcgtgcc
     2341 ggaattgctg acgccgccag agaaggccga gatcgtgacg gaggtttggg tgttgccaaa
     2401 gccgccgacg agaacgccgt tgcccagtga tcccgacgaa gaaatcgtgc cggagttgct
     2461 gatgccaccg gcgaaggtcg agatcgcgac tggtgcgccg tttgcaaggt cgccgacgag
     2521 aatgccagag ctcagctcgt ccaatggaga aatcgcgccg gactggctgt tgccaaggtc
     2581 tcccgacaca gaaatcgtgc cggaattgcg gacgccgccg ctgatcgtgg aattattctc
     2641 gacgatgatg ccgacgccgg aggtcgctgt gatggtgccg gcattgctca ccgtgccgcc
     2701 aatcgaacta ttgttgatcg taatgcccgc cgtggtcggc gaggtattgc cggtcgcggt
     2761 aatggtgccg ccaacctcgt tggtcacatc gccgctgacg gtcgagttct ggacgttgat
     2821 gcaattgatc gaacttccgc tcgaattgct caccgaggcg gccggaccga cttgattgat
     2881 agcgcaggca gcccaagccg gagtgccagc ggcgatcagc agcgcggcga ttgaacttga
     2941 ggcgaggagg tgacgtcggc cgctcgtaat tgcaatgcga ggcactatcg tcaccgccga
     3001 taacaaactc aggatacggg ctattatccg cttctacccc gacgcagcta gagccgagcc
     3061 tcatttcggc gcgccgtaac gcatgattgt tttcacttgt tgcggcgaaa acacaggcga
     3121 atcaacttgt tcacgcgacc gtgatgcatg tcaagaatgt cgggaaatac gtcagcgtga
     3181 gagtttcatt gcgtgcgaag gtgatcaggc ctgaaaaaat gcgctgatgg ttcgctgcct
     3241 gtcatgcggg acaatgttgc ccgtcgcgca ctatttcaaa gccgtgacaa gcgatcagat
     3301 ttgcggcaat aattttagcc tcgcagatcg cgcgcaatcg aggatgacta tggggcaagt
     3361 gcagagcggc gcggcggcgc tcgttccgct cggcgaagcg cttgcttata tcgagcgctg
     3421 ccgcggtgag ggccggctca tcgaagccga ggccgtatgc cggcaaattc tcgacgcgca
     3481 gccaaatttg cccgaagcct ggcatctcct cggcattacg gcgcatcagg ccgggcggct
     3541 ttcagacgct atcgctcacc tgacgcgggc aacgcaactc tcgccgcaaa atgcgctgtt
     3601 cctcagcaac cttggcgaaa tgtatcggca gggcgggcag ctcaagcgcg caatcgagac
     3661 cggccggcgc gccgttgccg ctgatcccgc tatggccgtt gcctggagca atctcggcgc
     3721 cgcgtatttc gaattgaaag actacaaaga tgccatcgag gcgaaccaac atgcaatcgc
     3781 cgccgatcca aatctggcgc agggtcattg caatctcggc aatgcgctgc atggcctgaa
     3841 gcgcttcgac gaagcgatca tcgcctatcg ccgcgcggtc gagataaatc cttcctatgt
     3901 tgatgctttg gccaatctcg gcacggcgct gcatcacaac ggtcattacg ccgaggcaat
     3961 ggcggcgctg cgccgggcga tcgcgctttc gcccgagcat gccaatgccc attcgggcct
     4021 tggcatattg cttttaatgc agggcgactt tgccgaggga tgggcggaat acgagtggcg
     4081 gcttcgttcg agcgaacgca aagggccacg ctttcccgtg cgtggctggc agggcgagag
     4141 cctcgtcggc aagcacatct atgtgcaggc cgagcaaggg ttcggcgata cactgcaatt
     4201 tgtccgttat atcccctggc ttgcaaagcg cgccggcaag gtgagcctgc gggtgcatca
     4261 ggaactcctg agtctgatgc gcgagagctt tcccggcatc gatgtttatg gtgaccgcgg
     4321 cgaccccggg cctttcgatt gcgacattgc gctgatgagt ctgccgcaat ttttcaaaac
     4381 acggttcgag acgattccgt cggaggtgcc ttatctccgg gcgtctgttg cgactacaga
     4441 acgctgggcg cagcgtcttg cgcccatgca cgggctcaaa gttggattgg tctggggcgg
     4501 taatcccgaa catgtcaacg ataggcgccg ctcgctcgac atagcccatt tcggaccgct
     4561 ccttgatgtg ccgggcgttt ccttcgcgag cctgcaattc gggccgcgtg tgatggattt
     4621 gaagaagctc aagggtacat cgaaagtcct cgatctttcg cgcgaactcg gcgacttcgc
     4681 caacaccgct ggcgcggtca tggcgctcga tgtggtcatc acgatcgatt cctcatcagc
     4741 acatctcgcc ggcgctttgg gcaagccggt gtggacgctg atttccgata tcgcggattg
     4801 gcgctggcgg ctgaatatcg aggacaatgt ttggtatccg aatatgcgtc tcttccgcca
     4861 gggaccaggc gaagattggt cgcatgtcgt cgcgcggatc gagacagcgt tgcggttggc
     4921 tggggccggc gacacgtccg ctcttctgcc gttctgggaa ttgggcgcgg gtcgcggggc
     4981 tgacgcgcga acaattctag aaatagagac gcagcgcgca cggattccgt cggccgttgc
     5041 tgcaccggaa agaccaacgt cgcctggcca tcttttcatc ctcgccgagc ggaagcgccg
     5101 cgctggcttc ctcggtgatg cggaggaatt ctcgcgccgc gcaattgagt ctcaaccagg
     5161 gaacgccgaa gcgatccata atctcggact taccgcgcac caatcaggca aagcgcatga
     5221 ggcgatcgcg catttgcgtc gggcgatcgc actggcgccg gatcaggcgc tttatcatgc
     5281 caatctcggt gaaatgtgcc gcctcgccgg cctcacggat gaggcgatca gcgaggggcg
     5341 gcgcgccgtc gaaatcgacc cgaactatcc cagtgcgctg agcaatcttg gcatcgcgct
     5401 tttcgaccag aacaagtttg aggatgcgct cgactatctt gaccgcgccg tggccttgca
     5461 acccgatttt gccaaagccc acagcaatcg tggcaacgcg ttgctaagac tacggcgctt
     5521 tgccgaagcc gaaccgtcat atcgtcgcgc cgtcgaactc gaaccggatt tcgccgacgg
     5581 atggaataat ctcggcacgt gcttgcgcga gttaaagcac tgtgaagacg ccgaggttgc
     5641 ctatcgcaag gcgctcgcct tgaagccgca cgatcccgac atcctcgata atctggcgct
     5701 tgcgctcaag gatctatcgc ggcttgacga ggccgccgaa tgcgcgcgcc gcgcgctggc
     5761 aatcgaagga cgcgatccga aacttcacat ccatgccggc gtcattttcc tcgaccaaca
     5821 taaaatcgac gaagctggca cggccgcggc gcacgcactc gcgttgaacc ccaacgatca
     5881 cgatgtgctc aatctgatgg ggcgggtcgc cttcgaacgc gagaaactcg acgagtccgt
     5941 agactattac cggcgcgcgc tcgcggtgaa gcccgatctt gcggacggcc acaacaacat
     6001 gggcaatgcg ttgaaggaat tcggccggct cgatgaagca cgcgaggcct atatcagggc
     6061 catcgatctc gatcctgcgt cgacgggagc ctacgtcaat ctcgccgatt ctatgaagtt
     6121 caggcctggt gaccccattc tcgcaaggat ggaagcgctc gccaacaaaa ccgagagtct
     6181 ttcgcagatc gaccggattc atctcgatta tgcgctgggc aaggcctatg ccgatcttgc
     6241 cgaccataag cgtgccctca ctcatttcat cgctggcagc gccgcaaaac gtcaaatgat
     6301 ctcttacgac gaagccgccg cgatgacatt cttcgacagg attgaaaacg tcttcacggc
     6361 cgatctgatg gcggaaaaat cgggccacgg ggatagttca aagcggccca tcttcattct
     6421 cgggatgccg cgctcgggta cgacactggt cgagcagatc ctcgccagcc atccgatggt
     6481 gcatggcgcg ggcgagctca ccgagttcaa cgaggccgtt gacagcatca aggggctgga
     6541 tggcgcggcg ctcacttatc cagaattcgt gccgccgctc ggtgcgtcag ccttgcgaca
     6601 attgggcgga cgctatctcg aattggtgtg caagcgttcg ccgtccggcg aacgcttgac
     6661 cgacaaaatg ccgtcgaatt attattttct cggcctcatc catctcgcgt tgccgaacgc
     6721 caagatcatt cacacggtgc gcgatccgat cgacacctgc atttcctgct tctcgaaatt
     6781 gttctcggcc gaccagaacc acacctatga cctcgccgaa ctgggccgtt actaccggcg
     6841 ctacgaacaa ttgatgacgc attggcgtcg ggttctgccg accgaaagct tccttgacgt
     6901 gcaatacgag gatgtcgtcg ctaacctcga aggcgcggcg cggcggatta tcgcctattg
     6961 cggcctcccc tgggatgatc ggtgtttggc gtttcatgaa acgaaccgtc cggtgcgaac
     7021 ggcgagcgca acgcaggtgc gccaacccat ttacaaaaac gcaattggcc gctggcgtgt
     7081 gtaccaagag ttccttggac ctttgctaac agcgctcgag cgttagccgc gccggttgat
     7141 ctgggatgtc gaacccggcc ctttctccgg gaagggtggt tggctgttcg taaccacgta
     7201 actctgaccc gcctgcgcct tggcgatgga ctgggcctga tggcgatgga accgcagggg
     7261 ctctgacccg aaccggaata ttctgcccgt cgcttcatcg agatcgcccg aggcgaccag
     7321 atcatcgacg gttgggccct gaagaaactt tgggttgaga gacagcatag catatagcat
     7381 caggccagaa acgcctggca tcgtactggc gatcaacttc agacttcagg tcatcagctc
     7441 gaatggtgct gaacgaccgc gagaaacgag cgtaagcatc gataagacgg tggtcaaatt
     7501 cgaacgcctt cattgccagt ttcgcccccc aatatttctt attatgccac aattaccgtt
     7561 ccggttggcc cgcctgatta gttgcgaggg gggtgcaaga cctccatcgg gaagggattg
     7621 ccatcctgaa tcgaactcgg ttgccaagcg gctcctcgtt cgcagtgcat ctgtgtgccg
     7681 acccatggcg ctcaccccca cagacacgac agcggcg
//
LOCUS       DASWOG010000420         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_321725,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000420 DASWOG010000000
VERSION     DASWOG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321725"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VGB93_08670"
     CDS             <1..731
                     /locus_tag="VGB93_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0146209.1"
                     /translation="STEIDNFVSRNEGTYLSRAQNIPKAPITLSVRVAFNPNVTTSWF
                     TSVMGIVNNVTMLAIILAGAAIVREREHGTMDHLLVMPVTPFEIAMSKIWANGLIIAV
                     ASALSLEIVVRQILKIPIAGSVPLFIAGVSIYLFFATAIGIFLATIARSMPQLGLLYI
                     LVAMPMNILSGSNTPLESMPPVLSAIMMFSPSTHFVLFAQSILFRGAGFSIVWPQFLF
                     VAFVGCLFLAIALMRFRTVAVQAG"
     gene            complement(963..1880)
                     /locus_tag="VGB93_08675"
     CDS             complement(963..1880)
                     /locus_tag="VGB93_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146210.1"
                     /translation="MDKLDAMAILLRVVEAGSLSAAAKRLGVPLATVSRRIAELEAHL
                     QTRMLNRSSRHLGLTEAGRLYVEASRRILEQVEDAERAAMGVHKDPKGRLTVTAPIVF
                     GRVHLIPIISDFMAEYPDIDLKLVLADRLLDLLESDVDVALRIGELGDSSLIASKVGT
                     VRRVTCASPSYLAAYGRPQIPEDLRNHTCVTFDNLASPEVWRFSSSKGDVLVPVRSRL
                     TVTTAEAAVAGVVAGMGLTRLLLYQIIEPRRAGQLELVLEDYELQPWPVSLVYAGQQM
                     LPRKLRAFLDFAAPRLKDRLRDEIPEAGS"
     gene            2103..>2591
                     /locus_tag="VGB93_08680"
     CDS             2103..>2591
                     /locus_tag="VGB93_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0146211.1"
                     /translation="MSQISAYPVTHHRTAKVDGVNIFYREAGPKDGPTVLLLHGFPTS
                     SHMFRNLIPYLADRYHVIAPDYPGYGQSDAPDFKSFSYTFEKFGELVDGLLQQLGVRS
                     YAMYVMDYGAPVGWQLALKHPDAITGLIIQNGNAYDEGLKEFWDPIKVYWKDGKKESR
                     DKL"
BASE COUNT          555 a          750 c          701 g          585 t
ORIGIN      
        1 tatcgacaga gattgataat ttcgtttcac gtaacgaggg tacctatctt tcgcgcgctc
       61 agaacatccc aaaggctcct atcacgctct cggtgcgtgt cgccttcaat cccaatgtca
      121 ccacgtcctg gttcacgagc gtcatgggaa tcgtcaataa tgtgacgatg ctggcgatca
      181 tcctggcggg cgcggccatc gttcgcgaac gcgagcatgg cacaatggat catttgctcg
      241 tcatgccggt cacgccattt gaaatcgcca tgtccaagat ctgggcaaac ggcctcatca
      301 ttgccgtagc gtcagccttg tcactcgaaa tcgtcgttcg gcaaattctt aaaatcccga
      361 tcgccggctc ggttcctttg ttcattgccg gcgtgagcat ctatctgttc tttgcgaccg
      421 cgatcgggat ttttctggcg acgatcgcgc ggtcgatgcc gcaacttggc cttctttata
      481 tcctggtcgc gatgccgatg aatattctgt cgggcagcaa cacgccgctc gaatccatgc
      541 cacccgtttt gagcgcgatc atgatgtttt cgccctcaac ccatttcgta ttattcgcgc
      601 agtcgatcct ttttcgtggc gcaggatttt ccatcgtctg gccgcagttt ctctttgtcg
      661 ccttcgtcgg ctgcctgttc ttggcgatcg cgcttatgcg ctttcgcacg gtggcggttc
      721 aggcggggta atgaacccga atttttgccg cagatcagat tgcgccactt ctacgaggcg
      781 caagaacagc attgcgaacg aataaaagct tcgcgcgtta actgaagctg gccgggtgat
      841 aaccggccgg agagggcgga cgcagttcat tttgccggct tctggctgcg acagcaacgc
      901 ataggtacgc gctaacgcct aacccattct cggccagcgc tattcggagt aatcctcatc
      961 aattaagacc cagcttcggg tatttcgtcg cgcaacctgt ctttaaggcg cggcgccgcg
     1021 aagtctagaa atgcgcgcaa ttttcggggc agcatttgct gacccgcata gacaaggctg
     1081 accggccagg gttgcagctc ataatcttcg agaacaagtt cgagctgtcc tgcgcgtctt
     1141 ggttcgatga tctgataaag cagcagccgc gtgaggccca tgccggcgac gacgcctgct
     1201 accgcggcct ccgccgtcgt caccgtcaat cttgagcgga ccggcacgag cacatcgcct
     1261 tttgaggatg aaaagcgcca gacctcgggc gaggcaaggt tatcgaacgt gacgcaggtg
     1321 tggttccgca ggtcttccgg gatctgcggc cggccatagg ctgcgagata ggatggactg
     1381 gcgcaggtca cacgacgcac cgtccctact tttgaagcga tcaaactgct atcgccgagt
     1441 tcgccgatcc gcaacgcgac atcaacatcg gattcgagaa gatccagcag tcggtcggcg
     1501 agaaccagtt tgagatcgat atcaggatat tcggccatga aatccgaaat aatcgggatc
     1561 agatgcacac gcccgaacac gatcggagcc gtcacggtca atcggccctt gggatcttta
     1621 tgaacgccca tcgcagcgcg ttccgcgtct tcgacctgtt cgagaatacg tctgctcgcc
     1681 tcgacataca gccgtcccgc ttccgtcagc ccgagatgcc gactcgaacg gttcaacatg
     1741 cgcgtttgca gatgcgcttc gagttccgct atgcgtcgac tgactgtagc cagaggaacg
     1801 ccgagtcgct tggccgctgc tgaaagactg ccagcctcga caaccctcag taaaatggcc
     1861 atcgcatcga gcttgtccat aattgcatct tcccaaaacg aacaatctag cttctcagga
     1921 tcgaggcgtt cccgcgacag gtgcaatgat atacgcctca gcccgggaga aacagcaagc
     1981 tgcagttcgg gcgctatcct ccgtcggagg acaggcggcc gtgtgacgtg aacgcccgat
     2041 ctcgaccgct cgagataccg gccaacatca accttctcga agatgattct caggagatgg
     2101 ctatgagcca gatatctgca tatcccgtga cccaccaccg caccgcgaag gtagacggcg
     2161 taaacatttt ctatcgcgag gctggtccga aagacggccc gaccgtcttg ttgctgcacg
     2221 gattcccgac gtcctcgcac atgttcagaa acctgatacc ctacttagcg gaccgttatc
     2281 acgtgatcgc gcccgactat cccggctacg gccaaagcga cgcgcccgac ttcaaatcgt
     2341 tttcctacac gttcgaaaag ttcggtgagc tggtggatgg tctgcttcaa cagctggggg
     2401 tgcggagcta tgccatgtac gtgatggatt atggcgcgcc cgttggttgg cagctggctt
     2461 tgaaacatcc ggacgctatc accggcctga tcatccagaa tggtaacgcc tacgatgaag
     2521 gattgaaaga attctgggac ccgatcaagg tctattggaa ggatggcaag aaggagagtc
     2581 gcgataaact t
//
LOCUS       DASWOG010000421         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_339414,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000421 DASWOG010000000
VERSION     DASWOG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339414"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VGB93_08685"
     CDS             complement(<1..792)
                     /locus_tag="VGB93_08685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0146212.1"
                     /translation="MPKLFATMQLRPEVKGPVAVVDIGSNSVRLVVYDGLTRSPTPIF
                     NEKALCALGHGVAKTGELAVPSVAKALAALKRYRVLIKIMNVQQVYAFATAAARDAAN
                     GPAFVADASNAIGAPVSLLSGRREAELSALGVISGFHEPDGIVADLGGGSLELIDIKG
                     TKVGKGITLPLGGLSLMDASNQSLRNAARIAQETLEDAKLLAKLRGRTLYAVGGTWRA
                     LAKLHMRQRNYLLSIMHGYNIPAREAADFAGLVERVNTEALIAVEA"
     gene            complement(801..2963)
                     /locus_tag="VGB93_08690"
     CDS             complement(801..2963)
                     /locus_tag="VGB93_08690"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY0146213.1"
                     /translation="MDNVAEGAVPHPDLAHSPERFINRELSWLEFNRRVLQEASNRNH
                     PLLEQLRFLSISANNLDEFFMVRVAGLRGQVRAGVSTPSEDGLTPAEQLAKIRERVGL
                     VAAEQQRRWGELRAELKANSIVLLEARDQEPADIAWLEQYFLQHVFPLLTPLAVDPAH
                     PFPFIPNLGFTVALELVEPRENRHLKALIRLPTKADRFVQMPPSGNIQRFIAIETLVG
                     MFTQALFPGYLVRGSGIFRVIRDSDIEVEEEAEDLVLLFQTALKRRRRGSVIRLEVDG
                     RMPEQLRNFVAEELDVAYDEIFVLDGMLAQNELSTLIGAVDRPDLKFKPYNPRFPERV
                     RENGGDCFVAIKQKDLVVHHPYESFDVVVQFLKQAAADPSVVAIKQTLYRTSSDSPIV
                     RALIEAAEAGKSVTALVELKARFDEEANIRWALDLERAGAQVVFGFIALKTHAKLSMV
                     VRREGNGLVTYCHLGTGNYHPVTARIYTDISFFTADPAIASDVSRVFNYITGYAEPAQ
                     LERMSISPINLKKRLLDHIAREAELARAGKPAAIWAKCNALVDPEIIDALYAASHAGV
                     KIDLIVRGICCLRPGVPGLSENIRVKSIIGRFLEHARVYAFGAGHGLPHSKAAVYISS
                     ADLMPRNLDRRVESLVPITNPTCHSQVLDQIMIANLIDNQQSYRVLPDGSSTRISAGK
                     EEPFNAHSYFMTNPSLSGRGKSLQESAPKTLLKRLEGR"
     gene            complement(3290..3511)
                     /locus_tag="VGB93_08695"
     CDS             complement(3290..3511)
                     /locus_tag="VGB93_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146214.1"
                     /translation="MSTKLNRPREIIEFCLAPLGISETSEEYKEAFRRLEHVIKTFQL
                     AAAREKQPVSVDFTHINTITIDERAHGDE"
     gene            complement(3536..4132)
                     /locus_tag="VGB93_08700"
     CDS             complement(3536..4132)
                     /locus_tag="VGB93_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0146215.1"
                     /translation="MSATNPRVADYPVDSQFIGRWSPRAFTGEAIPEAELRSLFEAAR
                     WAPSSSNLQPWRFFYALRDTPEFDTFLGLLFEGNQVWAKNAGALLVLASKTTNLPAGS
                     DTPVPTRSHSFDTGAAWAYFALEAQRLGWATHAMGGFDVARTGPTLNLPEDYRPEAAI
                     AVGRKGDPSILPESLQARELPNGRKPQSEFTFKGGFPA"
     gene            complement(4240..4551)
                     /gene="yidD"
                     /locus_tag="VGB93_08705"
     CDS             complement(4240..4551)
                     /gene="yidD"
                     /locus_tag="VGB93_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0146216.1"
                     /translation="MSRRVAHLLIRAYQLSLSAFIGRSCRHEPSCSAYTDEAIQRHGL
                     VLGVRLGAVRICRCHPWGTSGYNPEPEKVVPLRQRFGVFFARPHRRFECEAEAPKAPK
                     Q"
     gene            complement(4577..5029)
                     /locus_tag="VGB93_08710"
     CDS             complement(4577..5029)
                     /locus_tag="VGB93_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY0146217.1"
                     /translation="MLNDVYNNKILELAANIPRRGRLAAPDATARAHSKLCGSTVIVD
                     LTMENGRVTDFAHDVKACALGQASSSIMARHVIGATGPELRQLRDRVRAMMKENGPPP
                     EGKWADIAVLEPVRDYKARHASTLLTFEATVDAVEQIEAREKVSQDAF"
     gene            5238..>5735
                     /gene="folE"
                     /locus_tag="VGB93_08715"
     CDS             5238..>5735
                     /gene="folE"
                     /locus_tag="VGB93_08715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY0146218.1"
                     /translation="MDDVVKSLLDRQLPKTSTPSPIRPSREEAEAAVRTLLLWTGDDP
                     EREGLRETPARVVKAYEELFSGYKHDASEVLSRVFEEVQGYDDLVLVRDIPFNSHCEH
                     HMVPFIGKAHIAYYVGEEGVVGLSKLARLVDIYARRLQTQEALTAQIIGAIEKHLRPR
                     GAAVMI"
BASE COUNT         1180 a         1734 c         1704 g         1117 t
ORIGIN      
        1 cgcctcaacg gcaatcaggg cttcggtatt cacacgttcg acaaggccgg cgaaatccgc
       61 ggcttcccgc gccgggatgt tataaccgtg catgatgctc agcagataat tgcgctggcg
      121 catgtgcagt ttggcgagcg cgcgccaagt tccgccgacg gcatagagcg tacggccgcg
      181 cagtttggcg agcaacttgg cgtcttccag agtctcctgg gcgatgcgcg cggcgttgcg
      241 cagcgattga ttggaggcat ccatcagcga caggccgccg agcggcagcg tgatgccttt
      301 gccgaccttc gtacctttga tgtcgatgag ttcgagtgaa ccgccgccga gatcggccac
      361 gatgccgtcc ggctcatgaa agcctgagat gacgccgagc gcggaaagct ccgcctcacg
      421 ccggcccgaa agcaaactga caggcgcgcc aatcgcgttg ctggcgtcgg cgacaaaggc
      481 cggcccattt gccgcgtcgc gcgccgccgc cgtcgcgaag gcataaacct gctgaacgtt
      541 catgatcttg atcagaacgc ggtagcgctt gagcgcggcg agcgccttcg ccacgctcgg
      601 aaccgccaac tcgccggtct tggccacgcc atgacccaaa gcgcagagcg ccttttcatt
      661 gaagatcggc gtcggcgatc gcgtcagccc gtcataaacg acgaggcgca ccgagttaga
      721 accaatgtca acgacggcga ccggtccctt aacctccggg cgcaactgca tcgttgcaaa
      781 gagtttcggc atctgcagat tcaccggccc tccagacgtt tgagcagggt tttgggcgcc
      841 gattcctgca atgacttgcc gcggccggag aggctcggat tggtcatgaa ataggaatgc
      901 gcgttgaacg gctcttcctt gcccgcgctg atccgcgtgc tcgacccatc cggcaacacc
      961 ctgtagctct gctggttgtc gatgaggttc gcgatcatga tctgatcgag cacctggctg
     1021 tggcaggtag gattggtgat cggcaccagg gactcgacgc ggcggtcgag attccgcggc
     1081 atcagatcgg cggacgaaat atagaccgcc gccttcgagt gtggcagccc gtggccggcg
     1141 ccaaaagcgt agacgcgcgc atgttcgagg aagcgcccga tgatcgattt cacccggata
     1201 ttttccgaaa ggcccggtac gcccggccgc aagcaacaga tgccgcgaac aatcagatcg
     1261 atcttcacgc cggcgtggct cgcggcatag agggcatcga tgatctcggg atcaacgagc
     1321 gcattgcatt tcgcccagat cgcagccggc ttgccagccc gcgccagttc cgcctcgcgc
     1381 gcaatgtggt cgagcaaccg cttcttcaaa ttgatcggcg agatcgacat gcgctccaat
     1441 tgcgccggct cagcatagcc ggtgatgtaa ttgaagacgc gcgaaacatc gctcgcaatg
     1501 gccggatcgg ccgtgaagaa cgaaatatcg gtataaatcc gcgccgtcac cggatgataa
     1561 ttgccggtac caagatggca gtaggtgacg aggccattgc cttcgcgccg cacgaccatc
     1621 gatagcttgg cgtgcgtctt cagtgcaatg aagccgaaga ccacctgcgc accggcgcgc
     1681 tcaagatcga gcgcccagcg gatattggcc tcctcatcga agcgggcttt caattcgacg
     1741 agcgcggtaa ccgatttacc cgcctcagcc gcctcgatga gcgcccggac gatcggactg
     1801 tcggaggacg tgcgatacag cgtctgcttg attgccacta ctgacgggtc agccgccgcc
     1861 tgcttcagaa attgtacgac gacgtcgaac gattcgtaag gatgatggac gacgaggtct
     1921 ttctgcttga tggcgacgaa gcaatcgccg ccgttctcgc gaacccgctc ggggaaacgc
     1981 ggattatagg gtttgaactt caaatccggc cgatcgacgg cgccgatcaa tgtcgaaagt
     2041 tcgttctgcg cgagcattcc gtcgagcacg aaaatttcgt cgtaggcgac gtcgagctct
     2101 tcggcgacaa aattgcgcaa ttgctcgggc atacgcccat cgacctcaag ccggatgacc
     2161 gaaccgcggc ggcggcgttt cagcgccgtc tggaagagca gcacaagatc ttcggcctct
     2221 tcttcgacct cgatatcgct gtcgcggatg acgcggaaaa tgccggagcc ccgcaccaga
     2281 tagcccggaa aaagcgcctg cgtgaacatg ccgacaagcg tctcaatggc gatgaaacgc
     2341 tgaatgtttc ccgacggcgg catttgaacg aagcgatccg ccttggtcgg aaggcggatc
     2401 agcgccttga gatgacggtt ctcgcgtggc tcgaccaatt cgagcgcgac ggtgaaaccg
     2461 agattgggga tgaacgggaa cggatgcgcc ggatcgacgg ccagcggcgt caatagtgga
     2521 aaaacatgct gcaggaaata ttgttcgagc cacgcgatgt cggcgggttc ttgatcgcgc
     2581 gcctcaagga gaacgatgga attggctttc aattccgcgc ggagttcgcc ccagcgccgc
     2641 tgctgctcgg ccgcaaccaa accaacacgc tcgcgaattt ttgccaattg ctcggccggt
     2701 gtcagaccgt cttcggacgg tgtcgaaaca cccgctcgca cctggccccg caggcccgca
     2761 acgcggacca tgaagaattc gtcgaggttg ttggcggaga tcgagaggaa ccgcaattgc
     2821 tccagcaaag gatgattgcg attcgaggct tcctgcaaga cgcgccggtt gaattcgagc
     2881 cacgataatt cgcggttgat gaaccgctct ggcgaatgag cgagatcggg atgtggcaca
     2941 gcaccttcgg cgacattatc catgatcgag atggaggccg cgggcgacga gtcaccagta
     3001 agcgccgggt caaccggcgg cgggacctcg acaatctcct cgacttcgac ttccacttcg
     3061 acaggtttca gctgatcgcc tttggactgc acggcactct ctccttcgct tcgcccggcg
     3121 acgctgcctc ccgctgatga cgttaccatg actttcgcac gatatcgacg catcggcgaa
     3181 aaggttcaca ccttttcgat atttcgcaac accctgtaaa ataagggttt tccgatacat
     3241 ccctggcgtt gccgaatgcg aaatcgcaat gcggcgcgag acgataattt cactcgtcgc
     3301 cgtgcgcgcg ctcgtcgatc gtgatcgtgt tgatgtgcgt gaagtcgacg gagaccggtt
     3361 gcttctcgcg cgccgccgca agctggaagg tcttgatgac atgctccagc cggcgaaatg
     3421 cctctttata ttcttcgctt gtttctgaaa tgccgagcgg cgcgaggcag aattcgatga
     3481 tctcgcgcgg ccggttcaat ttggtgctca tggcaattct cctgagatgg atgcgctagg
     3541 cggggaagcc gcccttaaat gtaaattcgc tttgcggctt gcggccgttc ggcagctcgc
     3601 gagcctggag actctctggc aaaatgctcg gatcgccctt gcgaccgacg gcgatggcgg
     3661 cttcggggcg ataatcttcc ggcagattca gtgtcggccc cgtgcgcgcg acatcaaatc
     3721 cacccatcgc atgggtcgcc cagccgagcc gctgcgcttc gagcgcaaaa taagcccagg
     3781 ccgcgcctgt atcgaacgaa tggctgcgcg tcggaaccgg cgtgtcgctt ccggccggga
     3841 ggttcgtcgt cttggaggcg agcacaagca gagcgccggc gttcttcgcc caaacctggt
     3901 ttccttcgaa tagaagaccg agaaacgtgt cgaactccgg cgtgtcacgc aaagcataaa
     3961 aaaagcgcca cggctgcaaa ttggacgatg aaggtgccca acgcgctgcc tcgaatagac
     4021 tgcgcaattc cgcctctgga attgcctcgc ctgtgaacgc ccgcggtgac cagcggccga
     4081 taaattgcga atcgaccgga taatcggcca cacgcggatt ggttgcgctc acgtcattcc
     4141 ctcattccaa acggcttcgc aaagcccgat aaacacgccg gtctgttagt ggatttgtcg
     4201 gatcaacgac agtcaattgg cgatgcagcc aaacgcgcct tactgcttcg gtgctttcgg
     4261 ggcttcggcc tcgcattcga accgccgatg cggacgagcg aaaaatactc cgaacctttg
     4321 ccgcaacggg acaaccttct ccggctccgg attgtagccc gaggttcccc atggatgaca
     4381 gcgacagatt cggaccgcgc ccagccggac tcccaagaca agcccatggc gctgaatcgc
     4441 ctcgtctgta taggccgaac agctcggctc atgccggcaa gaccggccga taaaagccga
     4501 aagactgagc tgataggcgc gaatcagaag atgcgcgaca cgtcgcgaca taagctgcca
     4561 gccattgagc ttgccgtcag aaggcatcct gcgagacctt ttcccgggcc tcgatctgtt
     4621 caacggcatc gaccgtcgct tcgaacgtca gcagcgtcga agcatggcgc gccttatagt
     4681 cgcgcaccgg ctccagcacg gcgatatccg cccacttgcc ttcgggcggc ggcccgttct
     4741 ctttcatcat ggcgcgaaca cgatcgcgga gctggcgaag ttccggcccg gtcgcgccga
     4801 tgacgtgacg cgccataatc gaagaggagg cctgcccgag cgcgcacgcc tttacatcgt
     4861 gggcgaaatc cgtcacccgg ccgttttcca tggtcagatc gacgatcacg gtcgagccgc
     4921 acaatttcga atgcgcgcgg gccgtcgcgt ccggcgcggc aagccgccca cggcgcggaa
     4981 tattcgccgc gagttcgaga attttattgt tatagacgtc gttgagcatg acttctggct
     5041 gccctgcggc ggcggggatg accggccgcg acaaacccta tatagggatt acctagacga
     5101 agctcctata taagggaacc gaggctcgac gacgagattt ggcgcttttt tgggtcacac
     5161 tgcctcagga atgccgaatg cgaccttgtt tacctgaggt ttgttcttag ttttcaactc
     5221 tgccatggag gcaccacatg gatgacgtgg ttaagtcctt gctcgatcgc cagcttccca
     5281 aaacgtccac cccctccccc attcgcccca gccgcgaaga agcggaagcg gcggtgcgga
     5341 ctctacttct ttggaccggc gacgatccgg aacgcgaagg tctgcgcgag acgcccgcgc
     5401 gcgtcgtgaa ggcctacgaa gagcttttct cgggctacaa gcatgatgca agcgaggttc
     5461 tttcgcgcgt cttcgaggaa gttcagggct atgacgacct ggtcctcgtt cgagatatcc
     5521 cttttaactc gcattgcgag catcacatgg tgccattcat cggcaaggcg catatcgcct
     5581 attatgtcgg tgaagagggc gtcgtcggcc tgtcgaaact ggcccggctc gtcgatatct
     5641 atgcgcgccg cctgcagaca caggaagcgc tcacggcgca gattatcggc gccatcgaaa
     5701 agcacctccg cccgcgcggc gcggcggtga tgatc
//
LOCUS       DASWOG010000422         8729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_339421,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000422 DASWOG010000000
VERSION     DASWOG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339421"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGB93_08720"
     CDS             complement(<1..260)
                     /locus_tag="VGB93_08720"
                     /inference="COORDINATES: protein motif:HMM:NF016088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY0146219.1"
                     /translation="MYREILDVWTPLAPSPLVWIVLTIGAYLGGCCLQRRVGGAAYAN
                     PVLFAVVVVGLVVLATGTSYSTYFGGAQFINFLLGPATVALAV"
     gene            complement(242..652)
                     /locus_tag="VGB93_08725"
     CDS             complement(242..652)
                     /locus_tag="VGB93_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEY0146220.1"
                     /translation="MLAAIFIFLAAELLGELFRSVLHLPVPGPVIGMIALAAWLIWCG
                     RTTQPEPSALDRAADGILGHLGLLFVPAGVGIIGELAVIRREWLPILGGVIGSTILSL
                     AVTGLVLHHLLRREAAPKRAAALAIGDKACIAKS"
     gene            810..1772
                     /locus_tag="VGB93_08730"
     CDS             810..1772
                     /locus_tag="VGB93_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0146221.1"
                     /translation="MDYLAAVRAFVRAVDLGSFSKAAVEQELKVSTVSRYVSGLEADL
                     GVALFNRSTRRLHLTEGGADFYRRAVAILAEVEEARLAMHTLNASPRGVLRVNIPSAF
                     GRRHVVPHLKDFHDLHPQVRLDISLTDTVVNLIETGTDVAIRIGALEDSTLVAKKLAP
                     QRRIPVASPDYLGRHGAPNQPNDLTRHACLILAPQQGETWYFRRPVATEFANIIVSGD
                     VRANESEALLQLARDGLGIALLPTWIMADELVAGQLVPILDDWHWAIASGPERAIWGV
                     YPPKRIVAPKVRAFLAFFAARFGRPPYWERALPAHEITETGTIE"
     gene            1784..2653
                     /locus_tag="VGB93_08735"
     CDS             1784..2653
                     /locus_tag="VGB93_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146222.1"
                     /translation="MAAMFQLNGKIAVVTGAARGIGRAGAVALAQAGADVIGIDIAAF
                     VSPILDLAPASPEDLAETGRLVKDAGRRWHSVILDQRDIGTLRAAAAQIEKDFGGVDI
                     LFANAGIQAFKPILEMQDADWHDQIDVNLSGTANVLRAFAPLLVKRGGGRVIITSSTQ
                     GQHGTKFGAAYSASKWGLIGLMKSAALEFGPHKITVNALIPGLIDTALTRHEDRYAQV
                     IEDAGRAPAHDVARDEATAKAMLTTHSPLGVPWIEPEDVAPMVVFLASDAARMVSGAS
                     FSVTGGDSARFVA"
     gene            complement(2660..3121)
                     /locus_tag="VGB93_08740"
     CDS             complement(2660..3121)
                     /locus_tag="VGB93_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0146223.1"
                     /translation="MAPPVPPFTRETAIAKVRGAEDGWNSRDPERVSKVYTPDSIWRN
                     RAEFPRGRAEIIAFLTRKWARELDYRLIKELWAFTGDRIAVRFAYEWHDDSGQWFRSY
                     GNENWEFDAEGLMHHRYACINDLPIKEADRKFFWPLGRRPDDHPGLTDLGL"
     gene            3438..3826
                     /locus_tag="VGB93_08745"
                     /pseudo
     CDS             3438..3826
                     /locus_tag="VGB93_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
     gene            3951..5003
                     /locus_tag="VGB93_08750"
     CDS             3951..5003
                     /locus_tag="VGB93_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEY0146224.1"
                     /translation="MMDTATNPINLPTAHEAAPRHINFAIIPGLLLTATIAATGFALH
                     RLPGAGTFSPMILAIVIGIAFHNLVGTPARAKAGVTFSLRRVLRFAIILLGLQLTAQQ
                     VAEVGPKGLGVIVLTLVATFVFTKWLGKMLGVDRKLAELIAAGTSICGASAVIATNTV
                     TNAHDEDVAYAVACVTVFGSIAMFGYPLLPGLLHLSPHAYGLWAGASIHEIAQVVAAT
                     FQDGKAAGDFGTIAKLSRVTLLAPVVITLGLLARTHATSHGHHHQRTAAPIPWFVLGF
                     IALVGVNSVVTLPGAFKLAVVTLTTFLLSMALAAMGLETDIRKLRAKGLRPLLLGLAS
                     FLFIASFSLALVKMTA"
     gene            5148..5411
                     /locus_tag="VGB93_08755"
     CDS             5148..5411
                     /locus_tag="VGB93_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146225.1"
                     /translation="MLKKALALSLVVGFAFAASQAEALPLSSLSGASSPELTLVYGGC
                     GIHGHRGPYGHCRAGGQAGGYGRGRPCPPGWHIGGGGKHCWRN"
     gene            5481..6176
                     /locus_tag="VGB93_08760"
     CDS             5481..6176
                     /locus_tag="VGB93_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0146226.1"
                     /translation="MKSAIAIRHVQFEDLGTLAPALERAGYDVTYVDVDDDALASLDP
                     LAPDLLVVLGGPVGVYQTEAYPFLADELRLLETRLKASRPTLGLCLGSQLIAAATGAK
                     VAPAGHSEVGFSKLTLNDAGQASPLRHLEGVEVLHWHGDTFALPEGATLLASTELCRH
                     QAFALGPNILALQFHPEPDAAGLERWLIGYAGDLAASKLDPRLMRKDAALYCPALQHA
                     AANLIGDWLANLT"
     gene            6242..6499
                     /locus_tag="VGB93_08765"
     CDS             6242..6499
                     /locus_tag="VGB93_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEY0146227.1"
                     /translation="MAQHETKAQKEVVGRVMHEFKHGELESNRGGKVKSRKQAVAIAL
                     SEAGESKFESPEKNREHLKKTKEKERHGETAQAQKEGRAKH"
     gene            6739..>8729
                     /locus_tag="VGB93_08770"
     CDS             6739..>8729
                     /locus_tag="VGB93_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0146228.1"
                     /translation="MPESFEEAGSGFDAPALRPGGIAARASAGGGVRYLDGLNPEQRA
                     AVETLDGPLLVLAGAGTGKTRVLTARIAHILGTGRAFPSQILAVTFTNKAAREMKERI
                     ATLVGPAAEGMPWLGTFHAISTKILRRHAELVGLRNSFTIIDTDDQIRLLKQVLQAEN
                     IDDKRWPARTLAYQIDNWKNRGLAPAHVPAGEAAAFADGKGAKLYQHYQERLKILNAA
                     DFGDLLLESLRLLRENPDVLKQYQDRFRYMLVDEYQDTNTVQYLWLKLLAQGRNNICC
                     VGDDDQSIYGWRGADVDNILRFEKDFPGAKIVRLERNYRSTGHILDVAAGLIARNQGR
                     LGKTLFTDGDKGDKPTVSGVWDSEEEARVVGEEIEQLQRKGTHLDEVAILVRASFQMR
                     EFEERFVTLGLPYRVIGGPRFYERAEIRDALAYLRVTAQNDDDLAFERIINVPKRGFG
                     DASLQLLHDHARRERISLTQAARMMIETEELKPKQRQALRDFLADLNRWTGLIDQKQQ
                     GELAEIILEESGYTDMWQKDRTAEAAGRLENLKELVRAMDEFPSLAAFLEHISLVMDT
                     DSTDSAERVSIMTLHAAKGLEFETVFLPGWEEGLFPHQRSLDEQGRAGLEEERRLAYV
                     GLTRAKRRAKIYFATNRRIRGLWQTTIPSRFVDELPEAHV"
BASE COUNT         1699 a         2638 c         2683 g         1709 t
ORIGIN      
        1 acggcgagtg cgaccgtcgc cgggccaagc aggaagttga tgaattgcgc gccgccaaaa
       61 taggttgagt aagacgttcc cgtcgcgagg acgacaaggc cgacgaccac gaccgcaaac
      121 aggaccggat tggcataggc cgcaccgccg acgcgccgct gcagacagca acccccgaga
      181 taggcgccga ttgtcagcac gatccagacg aggggtgagg gcgcgagcgg cgtccagacg
      241 tctaggattt cgcgatacat gccttgtctc ctatcgcgag agctgccgcc cgtttcggcg
      301 cggcctcgcg gcggagcagg tggtgcagga cgagaccggt gacggcgagg ctcaagattg
      361 tcgagccaat gacgcctccg aggatcggca gccattcacg ccggatgaca gcaagttcgc
      421 cgatgatgcc aacgcctgct ggaacgaaga gaagaccgag atggccgaga atgccgtctg
      481 ccgcgcggtc gagcgcgctc ggttcgggct gcgtggtccg cccgcaccaa atgagccagg
      541 cggcgagcgc gatcatgcca atcacggggc cgggcaccgg caggtgcaaa acgctgcgga
      601 acaattcgcc cagaagctct gcggcaagaa aaatgaagat ggcggccaac atggcaaacc
      661 tccctgctgg cgaaaacatg cgctctcgca tcgcggcgtg aaacggctca gcgctcaaat
      721 ctcatttgca aaaaagggca taatgtcgac gaaaagcttg ataggatgcc aagcaacgcc
      781 gccattgttg cgcagacgga cagagactga tggattatct tgccgcggtg cgggcttttg
      841 tgcgcgccgt cgatcttgga agtttctcca aggcggcggt cgagcaggaa ctgaaagtct
      901 cgacggtgtc tcgctatgtg agcgggctcg aggccgacct tggcgtcgcg ctcttcaacc
      961 gctcgacacg acggctgcat ctgacggaag gcggggccga tttctatcgc cgtgcggtcg
     1021 cgatcctcgc cgaggtcgag gaagcgcgct tggccatgca tacgctcaac gcaagcccgc
     1081 gtggcgtgtt gcgcgtgaac atccccagcg cgttcgggcg ccggcacgtc gttccgcatc
     1141 tcaaggattt tcatgatctg cacccgcagg tgcggctcga catctccctg actgacacag
     1201 tggtgaattt gatcgagacc gggaccgatg tcgcgatccg catcggcgcg ctggaagatt
     1261 caacgctggt tgccaagaag ctggcgccgc agcgtcgcat tcctgtagca agccccgatt
     1321 atctggggcg ccatggggca ccgaaccagc caaacgattt aacgcgtcat gcctgtctca
     1381 tcctcgcgcc gcaacagggc gagacctggt acttccggcg acctgtcgcg accgagttcg
     1441 ccaatatcat tgtcagcggc gatgtccgcg ccaatgaatc cgaggccttg ttgcaactgg
     1501 cgcgcgatgg tttgggcatc gccttgctgc cgacatggat catggcggac gaactcgtgg
     1561 cgggtcagct tgttccgatt ctcgacgatt ggcattgggc catcgcctcg gggccggaac
     1621 gcgccatctg gggcgtctat ccgccaaagc gcatcgtcgc accgaaggtg cgtgcctttc
     1681 tcgctttctt cgccgcgcgt ttcggccggc cgccctattg ggagcgcgcg ctaccggcac
     1741 atgagatcac tgaaacgggg actattgaat aggagtttaa gccatggcgg caatgtttca
     1801 gcttaacggc aaaatcgctg ttgtcaccgg cgcggcgcgc ggcatcggtc gggctggcgc
     1861 tgtcgcactc gcccaggctg gcgccgatgt catcggcatc gatatcgcgg ccttcgtgag
     1921 tcctatcctt gatcttgcgc cggcgagccc cgaggatctc gccgagacag gccggctcgt
     1981 caaagacgcg ggacggcgct ggcattcggt cattctcgat cagcgcgata tcggcacctt
     2041 acgcgccgcg gccgcgcaga tcgaaaagga tttcggtggc gtcgacattc tcttcgccaa
     2101 tgccggaatt caggcgttca agccgatcct cgaaatgcaa gacgccgatt ggcacgatca
     2161 gatcgatgtc aatctctccg gcacggcgaa tgtgctgcgg gcctttgcgc cgctgctcgt
     2221 caaacgcggt gggggccgcg tcatcatcac gtcttcgacg cagggccagc atggcaccaa
     2281 attcggcgcc gcctattctg cgtcgaagtg gggcttgatc ggactgatga agtccgcggc
     2341 gctggaattt ggcccgcaca agattaccgt caatgcgctc atccccggcc tgatcgatac
     2401 agccctgacg cggcacgagg atcgctatgc gcaagttatc gaagacgccg gcagagcgcc
     2461 cgcgcatgat gtcgcgcgcg acgaagcgac cgccaaagcc atgctgacga cgcattcgcc
     2521 gctcggcgtc ccgtggatcg agccggagga tgtcgcgccg atggtggtct ttctcgcctc
     2581 ggacgccgca cggatggtca gcggcgcctc attctcggtg acgggcggcg acagtgcccg
     2641 ttttgtcgcg taatgcgcgt taaagcccga gatcggtaag ccccggatgg tcgtccgggc
     2701 ggcgccccaa aggccagaag aatttgcgat ctgcttcctt gatcggcaga tcgttgatgc
     2761 atgcgtaacg gtgatgcatc aagccttcgg catcgaattc ccagttctcg ttgccgtagg
     2821 agcggaacca ctggccgctg tcgtcgtgcc attcgtaggc gaaacgcacg gcaatccgat
     2881 cgccggtgaa ggcccaaagc tccttgatca gccgataatc caattcacgc gcccatttgc
     2941 gtgtcaaaaa cgcgatgatt tcggcgcggc cgcgcgggaa ctcggcgcga ttccgccaga
     3001 tcgaatccgg cgtgtaaact tttgatacgc gttcgggatc gcggctgttc cagccgtcct
     3061 ctgcgccgcg gaccttggca attgcggttt cacgggtaaa cggtggaacg ggcggtgcca
     3121 tggcgatctc ctgtactgat cggtaatcta tagaccattt ggtctatacg atgtcaagcc
     3181 gcaaccacat ttgtgctggc gttgatgcaa cgcgaggatg acgattaaat cacgttacaa
     3241 ctcgcacgca atctcggctc aattgtcgag cttcagattg tctttccggc cgaacgagat
     3301 gtgaagcggg cgcagatccc ctccccgaaa gcgtagctcg ctttcatcaa gaagtagcat
     3361 taaagaccgc caatcgctgc ggcgttgccg cggcctcggg ccggacatag cgccgcaaaa
     3421 gccagcaagg aggtcacgtg ccgcttatcc gtatcgatgc catcatcggc cgcacagaag
     3481 gcgaagttca aacgctgctc gatgcggcgc accgcgccat gctcgccgcg ttcaaagtcc
     3541 cggagcgcga ccgctatcag ctctatacgg aacatccggc atcgcatctg atcgcgcagg
     3601 acaccggcct taacattccg cgcacgcaaa attcgtcctc gtcacggtga cgacacgacc
     3661 gcgctcgcgc gccgagaaag agacgttcta taaattgctt tgccaggagc ttggggagca
     3721 ttgcgctatc gccacctccg acgtgatgat ctcgctcgtt cagaacacgg atgaggactg
     3781 gtctttcggc aacggccggg cgcagtttct gaccgccgag ctttaaagcg gtttcgagcg
     3841 aagcggatcg cgcctcgccg atcaatcaat ataattgatc gatataacag taataatgaa
     3901 ttatatttat cgatctgaat cctggacatt cggcgccaac acggagccga atgatggata
     3961 ctgcaacaaa tcccataaac ttgccgacgg cacatgaggc cgcgccgcgc catatcaatt
     4021 ttgcgatcat ccccggactg ctgctcaccg caaccatcgc ggcgacagga tttgccttgc
     4081 acaggctgcc gggcgccggg acgttcagcc cgatgatcct ggcgatcgtc atcggcattg
     4141 cgtttcataa cctcgtcgga acaccggcgc gggcaaaagc cggcgtcacc ttttcgctgc
     4201 ggcgggtcct gcgcttcgcc attatcctgc tcggcctgca actcacggcg cagcaggtgg
     4261 ccgaagtcgg gccgaaaggc ctgggcgtga ttgtgctcac gcttgtcgcc actttcgtct
     4321 tcaccaaatg gctcggcaaa atgctcggtg tcgaccgaaa gctcgccgaa ttgattgcgg
     4381 ccggtacgtc gatctgcggc gcctcggcgg tcattgccac caacacagtc actaatgcgc
     4441 atgacgagga cgtggcctac gccgtcgcct gcgtcaccgt tttcggctcg atcgcgatgt
     4501 tcggctatcc gctgctgccg ggcctgctgc atctatcgcc gcatgcctat gggctttggg
     4561 ccggcgcgtc gatccacgag atcgcacagg tcgtcgccgc gacttttcag gacggtaagg
     4621 cggccggcga tttcggcacg atcgccaagc tctcgcgcgt gacactgctg gcgcccgtgg
     4681 tcattacgct tggcttgctg gcgcgcaccc acgccacgag tcatgggcat catcatcagc
     4741 gtacagcggc accgatcccg tggtttgtgt tggggttcat cgcgctcgtg ggggtcaaca
     4801 gcgtggtgac attgcccggc gcgttcaagc tggccgtcgt gacgctgacg acatttcttc
     4861 tttcgatggc gctggccgcg atgggacttg aaaccgatat ccgtaagctg cgtgccaagg
     4921 gtttgcgtcc tttgcttctc ggcctcgcct cgttcctctt catcgccagt ttcagcctgg
     4981 ctctggtgaa aatgactgca tgatgacgat caagatgaaa cgaatgaaag aaggcaataa
     5041 gccgccgcgc gcttgaagct tggccgcggc ggttctatag gttcactcgg cttcaggccg
     5101 ttatactttc tatccggccc gggcctcagc attacgggga gatacggatg ctcaagaaag
     5161 cattggcact ttcacttgtt gtcgggttcg cgttcgctgc gtctcaggct gaggcgcttc
     5221 cattgtcgtc gctcagcggt gcatcatcgc cagaactgac gctcgtttac ggtggctgcg
     5281 gaatacatgg ccatcgcggt ccctatggcc attgccgcgc gggcggccag gctggcggct
     5341 atgggcgtgg tcgtccgtgt ccccccggct ggcacatcgg tggtggcggc aagcattgct
     5401 ggcgcaatta gaacttttat tttgactttc ctttgcaccc gccccacacg gcgggcgctt
     5461 tgtttttgga ttgaggtgat atgaaaagcg cgatcgccat ccgacatgtt caatttgaag
     5521 atctcggcac gcttgcgccg gcgctggaac gcgcgggcta tgatgtcacc tatgtggatg
     5581 tcgatgatga tgcgctggca tcgctcgatc cgctggcgcc cgatctgctc gtcgtgctcg
     5641 gcggtccggt cggggtctat cagacggagg cctatccgtt cctcgccgat gaacttcgtc
     5701 tgcttgagac gcggctgaaa gcgagccggc cgacgctcgg cctctgcctc ggctcacaat
     5761 tgatcgcggc ggccacgggc gcgaaggtcg cgccggccgg gcatagcgag gtcggcttct
     5821 cgaaactcac actgaatgac gcgggacagg caagcccgct gcgccatctc gaaggagtcg
     5881 aggttctgca ctggcacggc gacacgtttg cgctgccgga gggcgcaacg ctgctcgcct
     5941 cgacggaact ttgccgccac caggctttcg ccctcggccc caacatcctg gccttacaat
     6001 ttcaccccga gccggacgcg gccgggctgg agcgctggct tatcggctat gccggtgatc
     6061 tcgcggcgtc caagctcgat ccgcgcttaa tgcggaagga cgcagcgctg tattgtcctg
     6121 cgctgcagca cgccgcggcg aacctgatcg gcgattggct cgccaatttg acctgagttt
     6181 gccgtggatc atccggcggt tttgtgcgtt atttctcatc tcagccggtc aggagaacgt
     6241 tatggcgcaa catgagacca aggcgcaaaa ggaagtggtt ggccgtgtca tgcacgagtt
     6301 caagcacggc gaactggaat cgaaccgcgg cggcaaggtg aagagccgca aacaggccgt
     6361 cgcgattgcc ttgagcgagg ccggcgaatc caaatttgaa agcccggaaa agaaccgcga
     6421 gcatctgaaa aagacgaaag agaaagaacg ccacggcgag acggctcagg cccagaaaga
     6481 aggccgcgcc aagcattaat cgcccttaat ggcgagagcc gcgccgccgc ggcatctggg
     6541 catcagagaa agcctgcgta agcccgcacg ttctgctgtg cggcacgggc ctctgcgggt
     6601 cgacagaggt ggacaaagac cccacattag gcccaatgat tcgtctttcg ttccaacatt
     6661 tgaattggcg cgcattccta tcggccgggt gatttcatcc tgccggaatg tgcttgccgg
     6721 agagaagtgg gattgggcat tcctgagagt tttgaggaag caggctcggg cttcgacgcg
     6781 ccggccttgc gtccgggcgg tatcgccgcg cgggccagcg ctggcggggg cgtgcgttat
     6841 ctcgatggcc taaacccgga gcagcgcgca gcggtcgaga cgctcgacgg cccgctgctt
     6901 gtgcttgccg gcgccggcac cggcaagacg cgggtgctga ccgcccgcat cgcgcatatc
     6961 ctgggtaccg gccgcgcctt cccgagccag atcctcgccg ttacctttac caacaaggcg
     7021 gcgcgcgaga tgaaggagcg tatcgccacg ctcgtcggtc cggcggcgga aggtatgccc
     7081 tggctcggca cgttccatgc gatttcgacg aaaatcctgc gccgtcacgc cgagcttgtc
     7141 gggctgagga actcgttcac gatcatcgac accgatgatc agatccgttt gctgaaacag
     7201 gtgcttcaag ccgagaatat cgacgacaag cgctggccgg cccgcacgct cgcctatcag
     7261 atcgacaatt ggaaaaatcg tggccttgct cccgcgcatg tgccggcggg cgaagccgcg
     7321 gccttcgccg atggcaaggg cgcgaagctt tatcagcact atcaggagcg gctgaagatt
     7381 ttgaacgccg ccgacttcgg cgatctgttg ctcgaaagcc tgcggctctt gcgcgaaaat
     7441 cccgacgtgc tgaagcaata tcaggatcgc ttccgctaca tgctcgtcga cgagtatcag
     7501 gacacaaata ccgttcaata tctgtggctc aaactgctcg cgcagggccg caacaatatc
     7561 tgctgcgtcg gcgacgacga ccagtcgatc tatggctggc gcggcgccga tgtcgataac
     7621 atcctgcgct tcgagaagga tttccccggc gcgaaaatcg tccggcttga gcgcaattac
     7681 cgttcgaccg gccatatcct cgatgttgcc gcgggcctta tcgcgcgcaa tcagggccgg
     7741 ctcggcaaga cgcttttcac cgatggagac aagggcgaca agccgacggt ctccggcgtg
     7801 tgggattccg aagaggaagc ccgcgtcgtc ggcgaagaga tcgagcaatt gcagcgcaag
     7861 ggcacgcatc tcgacgaggt tgcaatcctg gtgcgcgcat cgttccagat gcgcgaattc
     7921 gaagagcgct tcgtcaccct tggcctgccc tatcgcgtca tcggcggccc gcgtttttat
     7981 gagcgcgccg aaattcgcga tgcgctcgcc tatctgcggg tgacggcaca gaacgatgac
     8041 gatctcgcct tcgaacgcat catcaacgtg ccgaagcgtg gcttcggcga tgcgagcctg
     8101 caattgctgc acgatcatgc gcggcgcgaa cgcatttcgt tgacgcaagc tgcgcgaatg
     8161 atgatcgaaa cggaggaatt gaagccgaag cagcgccaag cgttgcgcga tttcctcgcc
     8221 gatctcaacc gctggacggg actcatcgac caaaagcagc aaggcgaatt ggcggaaata
     8281 attctggagg aatccggcta caccgacatg tggcagaagg accgcacggc cgaggcggcc
     8341 ggccgcctcg agaacctgaa agaactcgtg cgcgcgatgg acgagtttcc gagcctcgcc
     8401 gcgttcctcg aacatatttc gttggtgatg gataccgaca gcacggattc cgccgagcgc
     8461 gtttcgatca tgacgcttca tgccgcgaag ggtctcgaat tcgaaacggt gtttctcccc
     8521 ggctgggaag agggcctttt tccgcatcag cgttcgctcg atgagcaggg ccgcgccggg
     8581 ctcgaagaag agcgtcggct tgcttacgtg ggtctcacgc gcgccaaacg ccgggcgaaa
     8641 atctatttcg caaccaatcg gcgcattcgc ggcctatggc agacgacgat cccgtcgcgc
     8701 ttcgtcgatg aattgccgga ggcgcatgt
//
LOCUS       DASWOG010000423         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_193656,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000423 DASWOG010000000
VERSION     DASWOG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193656"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGB93_08775"
     CDS             complement(<1..296)
                     /locus_tag="VGB93_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0146229.1"
                     /translation="MSQSHVYPVSAEWADRAFIDAKKYKELYQRSISDPDGFWRDEGQ
                     RRIHWIKPFTKVKNTSFGPGEVSIKWFEDGETNVAINCIDRHLKTRGDQVAIIW"
     gene            complement(499..1104)
                     /locus_tag="VGB93_08780"
     CDS             complement(499..1104)
                     /locus_tag="VGB93_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146230.1"
                     /translation="MIRRTRLALSAALILSCGFAAVPAVARSDAHHHARGHKAATKAK
                     KSKETHDTSESAKPTQVGSFGDWGAFVAATGDTKTCYVLAQPKTREPAKLRRDPAYIF
                     ISNRPGEHIHNEISITMGFPMKDGGEAEADVSGTTFELVSKGKNAWIKNPAEEGHFVA
                     ALRHNASLVVKAASSRGKVTTDTYSLAGLRQALDKVLHECR"
     gene            complement(1228..1968)
                     /locus_tag="VGB93_08785"
     CDS             complement(1228..1968)
                     /locus_tag="VGB93_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY0146231.1"
                     /translation="MKYISFIKGIRFGGCLIAGLFSSAAFAAPSPGAVQGVVELFTSQ
                     GCSSCPPADRLLTAMSRKADTITLTFAINYWDYIGWKDTLAAPEFTARQRAYAAARGD
                     HVYTPEAVVDGMFDAVGSDRDAIERAIAEGLSNTHALSVPAYLHEENGVLEIDIGRGT
                     GTAGIYVLRVAKSRSVEISRGENSGHQVTYTNVVRAIRKIGDWTGAPLKLKLMELRAD
                     DEGYVVLLQRGSEDRPGAILAAAKSGGL"
     gene            complement(2149..3318)
                     /locus_tag="VGB93_08790"
     CDS             complement(2149..3318)
                     /locus_tag="VGB93_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0146232.1"
                     /translation="MALALVAASAATPPAYADFRTCVAGLATEAEAHGVSAQVAQAAT
                     RNLTFNPDVLTAEKSQPEFNTPVWDYIAALVDEERVMDGRAAMAKYSRWFGVEERRFG
                     VSPYVVGAIWGVESNFGQNFGNKPVLQSLASLACSNSMRPGYYHREFMAAMEIVNEGG
                     ANPANFNGSWAGAFGNTQFMPSTFLRLAVDLDGDGRRDVINSVPDALGSTANYLHHSG
                     WVPGLPWGFEVKSSSGFEGRSNWHAKQPLSAWAARGITHVNGAPLTGEGAYGLFLPAG
                     PRGPAFLVSRNFSAIYSYNAAESYALAIALLSDRLRGLGPIVTPWPTNDPGLSRARRR
                     EVQRLLTEHGYDIGGKIDGVLGHKTQDAIADFERRNGMKTDGRASQSVLTALRSH"
     gene            3487..>4196
                     /locus_tag="VGB93_08795"
     CDS             3487..>4196
                     /locus_tag="VGB93_08795"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY0146233.1"
                     /translation="MSARRISLISSIPLLVGFVLLFIAAVSAVFLARQQQEIDAQLRH
                     TLEVDDDLLRIQTLIVDAESGQRGYLLTGRLSYLDPYNIAKRTLPGELRGLRSALGSA
                     VSEFDELSTTAAKKLAELQNTIDLRAANKPESALAIVNNDSGKRFIDRIRVILADMRG
                     TQETFLEARSVEAARLNESRRTILLTSVLLVIVLGLITLFSNLQRLRELQLVNDKLRQ
                     EAVERRAAETQVQQLQKM"
BASE COUNT          851 a         1243 c         1265 g          837 t
ORIGIN      
        1 tcccagatga tggcgacctg atcgccgcgc gtcttgagat gccggtcgat gcaattgatg
       61 gcgacattgg tctcgccgtc ctcgaaccat ttgatcgaga cttcgccagg accgaacgag
      121 gtgttcttga ccttcgtgaa aggcttgatc caatggatgc ggcgttgccc ctcgtcgcgc
      181 cagaagccgt cgggatcgct gatagagcgc tggtaaagct ctttgtactt cttcgcgtcg
      241 ataaaggcgc ggtcagccca ttccgcgctt accggataga cgtgagactg agacatactt
      301 cctcccagtg actttttata cggcgtccgc gcaagcaggc cgcgtcgtca cttcattttc
      361 gcaatcaggt tggcgcaatt cgccaggaac ggcccggttg caaacttccg cgcccattat
      421 gagccagcgg cagccgccta acaagacagc taaacggggg aggcgcgttg tagcgtcagg
      481 tgccgggatg ccggggcgtc agcggcattc gtgcagaacc ttgtcgagcg cctgacgcag
      541 accggcgagg gaatacgtgt cggtcgtgac cttgcctcgg ctcgaagccg ccttgacgac
      601 gaggctggcg ttatgccgca acgcggcaac gaaatggcct tcctcggccg ggttcttgat
      661 ccaggcgttc ttgcccttgg aaacgagttc gaacgttgtc ccggatacgt cggcttcggc
      721 ttcgccacca tccttcatcg ggaagcccat cgtaatcgaa atctcgttat ggatgtgctc
      781 gcccggacgg ttcgagatga agatataggc cgggtcgcgt ctcaatttgg caggctcacg
      841 cgttttcggc tgggcgagga cgtagcaggt ctttgtgtcg ccggttgccg caacgaaggc
      901 gccccaatcg ccaaaactgc cgacctgcgt cggcttggcg ctttcgctcg tgtcgtgagt
      961 ttctttcgat tttttggcct tggtcgctgc tttatggccc cgcgcatggt ggtgtgcgtc
     1021 gctgcgcgcg acggcgggca cggcggcaaa gccgcatgac aaaatgagcg cggccgacag
     1081 agccaagcgg gtcctacgaa tcatgggagg caacatatcc tgtttcaagg cggggaggcg
     1141 agccttgggg ctcatatctt caggctatga aacgggctga tcagggcaaa attccggctt
     1201 cggtgacgac aggcagatat tcagccgtta aaggccgccg ctcttggccg ctgcgaggat
     1261 ggctccgggc cgatcctctg aaccgcgttg aagcagcacg acatagcctt cgtcgtcggc
     1321 gcgcaattcc atcaatttga gtttcagcgg cgcgccggtc caatcgccga tcttgcggat
     1381 agcgcgcacg acattggtgt aagtgacctg atgtccggaa ttttcgccgc gcgaaatttc
     1441 gaccgagcgc gatttggcga cacgcaagac ataaatgccg gctgtgccgg tgccgcggcc
     1501 gatgtcgatt tcgaggacgc cattttcttc gtgcagatac gcgggcacgc tcagagcatg
     1561 ggtattgctc agcccctcgg caatagcgcg ctcgatcgca tccctgtcgc taccgacagc
     1621 atcgaacatc ccgtcaacga ccgcctcggg cgtataaaca tggtcgccac gcgcggcggc
     1681 ataggccctc tgccgtgcgg tgaactcagg cgccgccagc gtgtctttcc agccgatata
     1741 atcccagtaa ttgatggcga aggtcaacgt gatggtgtcc gccttgcgcg acatcgcggt
     1801 cagcagacgg tcggcgggcg ggcacgatga acagccttgc gaggtgaaca attcgaccac
     1861 gccttgaacc gcgcccggcg agggtgcagc aaaggcagca gagctgaaaa gccccgcgat
     1921 caaacagccg ccgaaacgga tgcctttgat aaagctgata tatttcatgc gcttaaatta
     1981 tttccgccag cgtctaattt aaaacaagct gggatcggtg ccagtcaatt ccgagtgaaa
     2041 ttcttgcgtt ttagaacact tcaaagtgaa gatttcgcgc cgttacagtc acgatttcga
     2101 gcgcagaaac acgattaaaa acaagccact caaatcggat cgcgcgggtc aatggctgcg
     2161 cagggccgtc aggacggatt ggctggcgcg cccatccgtc ttcatgccgt tgcgccgctc
     2221 gaaatcggcg atggcatcct gtgtcttgtg ccccaagacg ccatcgatct tgccaccgat
     2281 atcgtagccg tgctccgtga gcagacgttg gacttcgcgc cgtctggcac gcgataggcc
     2341 ggggtcgttt gtcggccagg gcgtcacgat cgggccgagc ccgcgcagcc ggtccgacag
     2401 gagcgcgatc gccaaagcgt aggattccgc agcgttgtaa gaatagatcg cgctgaagtt
     2461 ccggctgacg agaaaagcgg ggccacgcgg acccgccggt aagaacaggc cgtaggcgcc
     2521 ctcgccggtc aggggcgcgc cgttaacatg cgtgatgccg cgcgcggccc aggccgataa
     2581 cggctgcttc gcgtgccagt tggaccggcc ctcaaacccc gacgacgatt tcacctcgaa
     2641 gccccagggc agacccggca cccagccgct atggtgaaga taatttgccg tcgagcccaa
     2701 ggcgtcgggc accgaattga tcacgtcacg gcgcccatcg ccgtcgagat cgacggcgag
     2761 ccgcaggaaa gtcgaaggca tgaattgcgt attgccgaag gcacccgccc aggagccgtt
     2821 gaaattggca gggttcgcgc caccctcgtt gacaatctcc atcgcggcca tgaattcacg
     2881 gtggtaatag ccgggtcgca tcgaattgga gcaggcgagg ctcgccagcg attgcagcac
     2941 aggcttgttg ccgaagttct ggccgaaatt cgattcgacc ccccagattg cgccgacgac
     3001 gtaggggcta acgccgaatc gacgttcctc cacgccaaac cagcgcgaat atttcgccat
     3061 cgcggcgcgc ccgtccatca cgcgctcctc gtcgacaagg gcggcgatgt aatcccagac
     3121 cggcgtgttg aattccggct gcgacttctc ggcggtgagt acgtccgggt tgaacgtcag
     3181 gttgcgcgtc gcggcctgtg cgacttgcgc ggaaacgccg tgcgcctcgg cttctgtggc
     3241 gagacccgcg acacaggtgc gaaagtcggc ataggcggga ggggttgcgg cagatgccgc
     3301 gacaagggcc agggccatgg tggcgaagcc aggagacgag aatcgcatca tggtgtgagt
     3361 ttaccgcaaa cctgcagagc cagccatgcg cctcacgatc cctctgccac tgcgccagca
     3421 acttcccttt tacgagcatg ctcgcttcac gtagtctacg agaatacagg tgggattaag
     3481 taaaaaatgt cggcgcgcag aatatccttg atttcatcga tccctctgct cgtcggcttt
     3541 gttcttttgt tcattgcggc cgtcagcgcg gtttttctcg cccggcaaca gcaggagatc
     3601 gacgcgcaac ttcgccacac tctggaagtg gacgatgact tgctgcgcat ccagaccctc
     3661 atcgtggatg cggaatcagg ccagcgcggt tatctgctca ccggcaggct gagctatctc
     3721 gacccctata atattgcaaa acgtacgttg ccgggagaat tgcgcggact gcggtccgcg
     3781 cttgggagcg ccgtgtccga gttcgacgaa ctcagcacca ccgccgccaa gaaactcgcg
     3841 gaactgcaaa acacgatcga tctacgcgcg gcaaataagc ccgaatccgc cctcgccatt
     3901 gtgaacaatg acagcggcaa gagatttata gatcgcatcc gtgtgatcct tgccgatatg
     3961 cgcggcacgc aggaaacttt tcttgaagcc cgctccgttg aggcggcgcg cctcaacgaa
     4021 agccggcgga caattcttct cacaagcgtc cttctggtga tcgtgcttgg ccttataact
     4081 ctcttcagca atctccagcg cttgcgcgag ctgcaattgg tcaatgacaa gctgcggcag
     4141 gaggcggtcg agcgccgcgc ggcagagacg caggttcagc aattgcagaa gatgga
//
LOCUS       DASWOG010000424         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_64720,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000424 DASWOG010000000
VERSION     DASWOG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64720"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGB93_08800"
     CDS             complement(<1..579)
                     /locus_tag="VGB93_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146234.1"
                     /translation="MFDARRVKAFGQVPLTFVVLMALPTFGFAQAPAPQAAPSVAAPA
                     SPAAPTAAPTQAPPAPPPSAAASPAKAPPAGAPVATQPAISAAKPALAAPDSNAPVSI
                     TSQLLPQNLSAWGMFLNADIIVKIVMVGLALASLATWTVWLVKSLELANAKRKASRQL
                     GILAHAATLDAARTDLGKGRGPVVRMVDAAAAE"
     gene            complement(803..>1814)
                     /locus_tag="VGB93_08805"
     CDS             complement(803..>1814)
                     /locus_tag="VGB93_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY0146235.1"
                     /translation="LRRWTDLAARLSTGAANRATSADVLKTDSGEALGLDPNRLTLTF
                     GFGASLFEKAGKDRYDLKARKPEALIDLPYFNGDQLVEEQTGGDLSVQACANNPQVAF
                     HAIRQLAREAHGIASIRWAQSGFSAQYGDKAPRNVMGFKDGTINPQTNLDYDRFVWVG
                     DEGPAWMHGGSYVVARLVRIAIEHWDRMDLNYQQQTIGREKFSGAPLGGRKETDPLDL
                     KAVDKDGNPLIPPTAHARIASAAMNDGAQLLRRGYSYNNGATFIAERWPPWRQGIEYD
                     AGTFFICYQRDPRTAFVKIFDKLSKLDAMNQFVTHVGSGLFACPPGIGEGGFIGDKLF
                     AT"
BASE COUNT          329 a          546 c          595 g          344 t
ORIGIN      
        1 ctccgccgcg gctgcatcga ccatacgcac cacgggcccc ctgcccttgc cgagatcggt
       61 ccgcgctgcg tcgagcgtcg cggcatgcgc gaggatgccg agctggcgcg atgccttgcg
      121 cttggcgttg gcaagttcga ggctcttgac gagccatacg gtccaggtcg cgagcgaggc
      181 gagcgccagc ccaaccatca cgatcttcac gatgatatcg gcgttcagga acatacccca
      241 ggccgacaga ttttgcggca ggagttggga ggtgatcgag accggcgcat tcgagtccgg
      301 cgccgcgagc gccggctttg cggccgagat cgccggctgg gtcgcgacgg gtgcgccggc
      361 tgggggcgcc tttgcgggtg acgcagcggc cgaaggcggc ggggcggggg gggcttgcgt
      421 cggggctgcg gtaggcgcgg ccggggatgc tggtgcggcc acgctcggag cggcctgcgg
      481 ggctggcgct tgggcgaaac cgaacgtcgg cagcgccatc agcacgacaa atgtgagagg
      541 cacttgtcca aaggccttta cgcgacgcgc atcaaacatg aattccgtcc ttgctctcag
      601 ccttcccgcc cgtgtccgtg ccaacttgcg tggcaaggcg ccaagggtca atctgcgcag
      661 ggaaatattc gcgcgattga tgacttcttc ggtctttctt ccaagtgcgc agcccttcta
      721 cagccagcgc attggcaagt cttctcggat tatccgagaa agtttgtaag caaacgcggg
      781 aaaaattccc gagcgctgcc gctcaagtcg caaacagctt gtcgccaatg aatccacctt
      841 cgccaatgcc cggcgggcac gcgaacaggc cactgccgac atgcgtgaca aattggttca
      901 tcgcgtcgag cttcgagagc ttgtcgaaga ttttgacgaa ggccgtacgc gggtcgcgct
      961 gatagcagat gaagaacgtg ccggcatcat attcgatgcc ctggcgccac ggcggccaac
     1021 gttcggcgat gaaggtcgcg ccgttgttat aggaatagcc gcggcgcaga agctgtgctc
     1081 cgtcattcat cgccgctgag gcgatgcgtg cgtgcgcggt cggtgggatc agcggattgc
     1141 cgtctttatc aacggccttg aggtcgagtg gatcggtttc cttgcgtccg ccgagcggcg
     1201 cgccggagaa tttctcccgt ccgattgtct gctgctgata gttgagatcc atgcggtccc
     1261 agtgttcgat ggcaatacgg acgagccgcg cgacgacgta gctgccgccg tgcatccagg
     1321 ccggaccttc gtcgccaacc cagacgaagc gatcgtaatc gagattcgtc tgcggattga
     1381 tcgtgccgtc cttgaatccc atcacgttgc ggggggcctt atcgccatat tgggcggaaa
     1441 aaccgctctg cgcccagcgg atcgaggcga tgccgtgcgc ctcgcgcgcc aattggcgaa
     1501 tggcgtgaaa cgcgacttgc ggattgttgg cgcaggcctg gacagagaga tcgccaccgg
     1561 tctgctcttc gaccaattga tcgccgttga aatagggcag atcgatcagc gcctcgggtt
     1621 tcctggcttt gaggtcgtaa cgatccttgc cggccttctc gaaaagagaa gccccgaagc
     1681 caaaggtcaa cgtcagccga tttggatcga gccccaaagc ctcgccgctg tcggtcttca
     1741 acacgtcggc cgaagttgcg cgattggcgg cgccggtcga aagccgcgcg gcaagatcgg
     1801 tccagcgccg caac
//
LOCUS       DASWOG010000425         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_193728,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000425 DASWOG010000000
VERSION     DASWOG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193728"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..838)
                     /locus_tag="VGB93_08810"
     CDS             complement(71..838)
                     /locus_tag="VGB93_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0146236.1"
                     /translation="MTAIAKRRFGADEAEAGPAHGNTSLLLFESLPNLAGVPDIFAAL
                     TEHESKQVMAAGESIRFAPGDLLFRQGDPHRGIFILRDGIVRSLYSAPNGREITLANW
                     TPGNFVGGPDIFGEAPHVWSGVGVKSGEAIRLPGPVVRRLMSEIPNFAIGLVQGLAYK
                     GKCYSSLLQMLGTRSVVERLALLLLNLTTLKGVESENAIVIADMPSHEELAAMVGATR
                     QWVSINIEKFRKRGLIDVGDRRLVILRHDALRAIAGC"
     gene            1170..>1807
                     /locus_tag="VGB93_08815"
     CDS             1170..>1807
                     /locus_tag="VGB93_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0146237.1"
                     /translation="MNNNTFFRHRVRYRGVGTSLLGFLMATSVLAGGASQAFADDSAS
                     IHRLEAEIQRIEARHQQEIGALRAEIHHLRHREGGPIYVNKDEEPGALLPHVIESEKG
                     PVHFGFASGDGQNTVELTGRLHIDTGGYLDTNRNTTSSGFASHGLASGINFRRARIGV
                     QGIFLGDWAYGLIFDLGGSSDGYNGGEAQNSGTSTSGIENAFITYNGFYKHGG"
BASE COUNT          411 a          513 c          517 g          366 t
ORIGIN      
        1 gagaccagcc aatgcccaaa tacccagacg ttttagaaat aggctcacgg aattttctcc
       61 tgttcggtaa tcagcagccg gcgatcgcgc gaagcgcgtc atgccggaga atgacgaggc
      121 ggcgatcgcc gacgtcgatg agcccgcgct tgcggaactt ctcgatattt atggaaaccc
      181 attgccgcgt cgcgccgacc attgccgcga gttcttcatg cgaaggcatg tcggcgatga
      241 cgatggcgtt ttcggattcg acgcccttga gcgtcgtgag attgagaaga agaagcgcca
      301 gccgttcaac gacggaacgt gtgccgagca tctgcagcag cgacgaatag catttgccct
      361 tgtaggcaag accctgcacg aggccgatcg cgaagttcgg aatttccgac atcagacggc
      421 gcacgacagg gcccgggaga cggatcgctt cgccactctt cacaccgacg cctgaccaga
      481 catgcggcgc ttcgccgaag atatctgggc cgccgacaaa attaccgggt gtccaattgg
      541 caagcgtgat ctcgcgccca ttcggtgccg aatacagact gcggacaatt ccatcgcgca
      601 gaatgaagat accgcgatgc gggtcgccct gccgaaagag aagatcgccc ggcgcgaagc
      661 gaatggattc gcccgcggcc ataacttgct tcgattcgtg ttcagtcagt gccgcgaaaa
      721 tatcggggac gccagccaga tttggcagtg actcgaagag caataacgac gtgttgccat
      781 gcgcagggcc ggcttccgcc tcatcggcgc cgaatcgtct ttttgcgatt gcagtcaaaa
      841 gtgttcagct ccccggcgcc gcattcggat gggcggagca agggcttaaa atcccgcatc
      901 aaattataaa gtcaatggat ttaatatatt aatggcgagg atatcggcgc ggcgatggcc
      961 ataagcaccc tgcaacgctt aggctgttga attggcgtgg agaagatcgt ttttgctcca
     1021 agacggcaca aaacgtgaca tatttatgaa tatgggcgcc aaccgtggcc aaaataacac
     1081 gcgatttaac tcccacacct aatgtctagt tacttagttg actaaggccc cctttttaaa
     1141 cttgagttta taaatccaga gggggttcca tgaacaacaa cacttttttc cggcaccggg
     1201 tccggtaccg cggagtaggc acgagcctgc tcggctttct aatggcgacg agcgtcctcg
     1261 ctggcggcgc cagccaggct tttgctgacg acagcgcatc catccaccgg ctggaagctg
     1321 agattcagcg catcgaagcg cgccatcagc aggaaatcgg ggctctgcgc gccgaaatcc
     1381 atcatttgcg tcatcgcgaa ggcggcccga tctacgtcaa caaggacgag gagcccgggg
     1441 cgctgctgcc gcatgtcatc gagagcgaaa agggcccggt gcatttcggc ttcgccagcg
     1501 gcgacggcca gaacacggtt gagcttacgg gtcgtctgca tatcgatacc ggtggttatc
     1561 ttgataccaa ccggaatacg acctcgtctg ggttcgcttc gcatggtctt gccagcggca
     1621 tcaacttccg ccgcgcccgt atcggtgtcc agggcatctt cctcggcgat tgggcctacg
     1681 gcttgatctt cgatttgggc ggatcgagcg acggctataa cggcggcgag gcccaaaatt
     1741 ccggcaccag cacgtcgggc atcgaaaacg ctttcatcac atacaacggc ttctataagc
     1801 acggcgg
//
LOCUS       DASWOG010000426         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_230221,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000426 DASWOG010000000
VERSION     DASWOG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230221"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..548
                     /locus_tag="VGB93_08820"
     CDS             306..548
                     /locus_tag="VGB93_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146238.1"
                     /translation="MSKLSREERLQIMLTGEELRALDDWRFTKRMPSRAAAVRELLRR
                     GLAAEGFDIAGKGSKSEDFGMIDPQQPTLRETKKGS"
     gene            713..982
                     /gene="rpsO"
                     /locus_tag="VGB93_08825"
     CDS             713..982
                     /gene="rpsO"
                     /locus_tag="VGB93_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0146239.1"
                     /translation="MSITAERKDALIKEYATKSGDTGSPEVQVAILTERISNLTGHFK
                     THMKDNHSRRGLLKLVSLRRQLLDYVKRKDESRYKTLIERLGIRR"
     gene            1314..>1767
                     /locus_tag="VGB93_08830"
     CDS             1314..>1767
                     /locus_tag="VGB93_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0146240.1"
                     /translation="MFEIHREELDWAGHKLVLETGRIARQADGAVLATYGETTVLATV
                     VGAKAPKPGQDFFPLTVNYQEKAFAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPL
                     FPDGYRNDTQVVVTVLSHDLENDPDILSLVAASAALTLSGIPFMGPVGG"
BASE COUNT          385 a          518 c          505 g          359 t
ORIGIN      
        1 gtcgtttttt gttgacggct gcatgtttgt ctccgagcta aaacaaaaac tacttatcga
       61 tagtagaagc aaatgtagaa tcgtcaatat tctttgacat atatactttt acaacatgca
      121 gccaattaat ataaatacac actatactat aatgctcatt tgtcagatta cgcggtcttg
      181 ccggtgaagg ccctaaagga tggcaacacg ttgattacgg tttttgcata atcaactgtg
      241 agtgagtaat tgacgacggc agcgctaaag gcatattatg ggcaattgcc gtccgcggat
      301 caaccatgtc caaattgtct cgagaagaac gattgcagat catgctgacg ggcgaggagt
      361 tacgcgcgct cgacgactgg cggtttacca agagaatgcc gagtcgggct gccgcggtcc
      421 gtgagctttt gcggcgcggt ctcgccgcgg aggggttcga tatcgccggt aaaggctcca
      481 agtcggagga ttttggcatg atcgatccgc agcagcccac gctgagggaa accaaaaaag
      541 gatcttgagg cagccgcatt acccccgggc gtgccgcttc catccaccgc aatttcctgt
      601 ataaagcccc gatcgctggc cctttggccg gcgcgctccc tcaaaacctt gctggacgac
      661 atcccggcct ggccgcggcg gaggcatttt tcaaaacctg aaaggattaa cgatgtcgat
      721 cacggccgag cgcaaggacg cgctcatcaa ggaatatgcg acgaaatccg gcgacactgg
      781 ctcacctgag gtgcaggtcg cgatcctcac cgagcggatc agcaatctca ccgggcattt
      841 caagacccat atgaaggata atcattcgcg tcgcggtctg ctcaagctcg tctcgctacg
      901 tcgccagctt ctcgactacg tgaagcgcaa ggacgaatcg cgctacaaga cgctgatcga
      961 gcggctcggc atccgtcggt aaggcgatcg ggctcgcgct tcatggcgga gcgccgaaat
     1021 tgccgccccg cctatgacca agcttgaaag ccgccgcccg cgggcggcgg agataaagtg
     1081 gcgacgccgc aggtttccgg ctcgcgaaaa tgttctaaac tcggctggtg cattgcgcac
     1141 cgagcggagc gggcccccgc tccatcgaga agacggcagg gcggcatggg gccgccgctg
     1201 tcgatgaaag accggagcca tggcaggatc gccaggcgct tctcggaagc gagaagcctc
     1261 ttgccattct gcacatggcc tttggtcttt gcctcacgca aagagaatgt ccaatgttcg
     1321 aaatccatcg tgaagaacta gactgggccg ggcacaagct cgtgctcgaa accgggcgca
     1381 ttgcccgtca ggcggatggc gccgtgctcg cgacctatgg cgagaccacc gtgctcgcca
     1441 ctgttgttgg cgccaaggca ccgaagccgg ggcaggattt cttcccgctt accgtcaatt
     1501 atcaggagaa ggcttttgcc gccggccgca tccccggcgg ttatttcaag cgcgaaggcc
     1561 gcccctctga aaaagagacg ctggtctccc gcctgatcga ccgaccgatc cgcccgctgt
     1621 tcccggacgg ctatcgcaac gacacgcagg tggtcgtcac cgtcctctcg cacgacctcg
     1681 aaaatgaccc ggacattctg tctctggtcg cggcgtcggc ggccctaacc ctgtccggca
     1741 ttcccttcat gggtccggtc ggcggtg
//
LOCUS       DASWOG010000427         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_101928,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000427 DASWOG010000000
VERSION     DASWOG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101928"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGB93_08835"
     CDS             <1..325
                     /locus_tag="VGB93_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0146241.1"
                     /translation="GTSGTVTTIGGIFLNLPRYDRRRVDGLWMSDAEIDTTVKRLRRM
                     SYAERAANGCIGVERADLVLAGCAILEAIRRAFPARRVRIADRGLREGILLQLMDTEL
                     ARSSR"
     gene            322..1020
                     /locus_tag="VGB93_08840"
     CDS             322..1020
                     /locus_tag="VGB93_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY0146242.1"
                     /translation="MNAKEPSGTGRASLKTRVKTARKRSLASTLWLDRQLNDPYVARA
                     KAEGYRSRAAFKLLEIDARYHVLKRGQKVVDLGAAPGGWSQVAAAKVGAAEGAGKVFG
                     IDLLPVETIPGVDFRQLDFLIPEAPQILTEWLGGPADVVLSDMAGNTTGHRKTDHLRI
                     VGLVELAADFAAEILAPGGAFLAKVLQGGTETSLLATLKRQFAEVRHVKPKASRADSS
                     ELYVLATGFRGRAD"
     gene            complement(1017..2453)
                     /locus_tag="VGB93_08845"
     CDS             complement(1017..2453)
                     /locus_tag="VGB93_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146243.1"
                     /translation="MPRYGLTAFIVACALFMENLDGTVISTSLPAIAADLHQDPISLK
                     LALTSYLLSLAIFIPASGWAADRFGARTIFRAAIVVFTLGSIFCGFSSTLWQFVLARI
                     VQGVGGAMMVPVGRLVLLRTVPKSEVINALAYLTIPALLGPLFGPLVGGFITTYFHWR
                     WIFFINVPIGAIGIVLVSLHIKNLRGDYWPLDVSGFLLSGTGLATLIFGMTLFGRNIV
                     SLEVVLALMGFGTLLLVAYYFHARRAKFPILDLSLLKVPTFRASLTGGSLFRVGVGAT
                     PLLLPLMLQIGFGLNAFQSGSMTFITSLGAMAMKTTAAPILRTFGFRHVLLFNSVLSA
                     TILGCYGFFTPTTPHLLMMGLLLFGGFFRSLEFTAINAITYADIDHNTMSRATSFSSV
                     VQQLSLSVGITIGAFVLQTSEAIHHTAHVTAADFRWAFLGIALISASSVLVFRALPAN
                     AGIDLAGSRQKKTADPEKELAEAEETEG"
     gene            2726..>3354
                     /locus_tag="VGB93_08850"
     CDS             2726..>3354
                     /locus_tag="VGB93_08850"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0146244.1"
                     /translation="MAALQRGVIKEALTFDDVLLLPGHSEILPSNADLKTRLTPEIML
                     NLPIISAAMDTVTEARLAIALAQAGGIGVIHRNMDPEAQAEEVRKVKRYESGMVVNPI
                     TIVPDATLADALALMKRHGISGIPVVEHGPVGKPARLVGILTNRDVRFADNPNQPVSE
                     LMTKKVITVTEGVPQDEARRLLHQHRLEKLVVIDEEGRCVGLLTVKDIE"
BASE COUNT          681 a         1003 c         1053 g          617 t
ORIGIN      
        1 cggaacgtcc ggtacggtca cgacgatcgg cggcattttt ctgaacctgc cacgctatga
       61 ccggcgccga gttgacgggc tctggatgtc ggacgctgag atcgatacga cggtcaagcg
      121 gctgcggcgt atgtcttatg ccgaacgcgc cgctaacggt tgtattggtg tggagcgcgc
      181 cgaccttgtg ctcgcgggct gcgccatatt ggaagcgatc cgccgtgcct ttccggcgcg
      241 tcgcgtccgc attgctgatc gcggtctgcg ggaaggcatc ctgttgcaat tgatggacac
      301 cgaactggct cgatcctcgc gatgaatgcg aaagagccgt ctggcaccgg tcgcgcctcg
      361 ctcaagacgc gagtaaaaac ggcacgcaag cgttcgctgg cttcgacgct ctggctcgat
      421 cgccagctca acgatcctta tgtcgcgcgc gccaaggccg aggggtaccg gtctcgcgcc
      481 gcctttaaac ttctcgagat cgacgctcgc taccacgtcc tcaagcgcgg gcagaaagtg
      541 gtcgatcttg gagcagcccc cggtggctgg tctcaggtcg ctgccgccaa ggttggcgcg
      601 gccgagggcg ccggcaaagt gttcggcatt gatcttctgc cggtcgaaac gattccgggc
      661 gtcgattttc gtcagcttga tttcctgatc cccgaagcgc cgcagatctt gaccgaatgg
      721 cttggcggtc ccgccgatgt ggtgctctcc gatatggccg ggaataccac gggccatcgg
      781 aagacggatc atttgcgcat cgtcggcctg gtcgagcttg ccgccgattt cgcagccgag
      841 attttggcgc cgggcggcgc ctttctcgcc aaggttttgc agggtggcac ggagaccagt
      901 ctgcttgcca cgctcaaacg tcagttcgct gaggtcagac acgtcaagcc caaggcaagc
      961 cgggcggatt cgtctgagct ttatgtcctc gcgaccggct ttcgcggccg cgcggactag
     1021 ccctccgtct cttcagcttc cgcaagttcc ttctctgggt cggctgtctt tttctgccgc
     1081 gacccggcga gatcgatgcc ggcattcgcg ggtagcgcgc ggaacaccag caccgaactt
     1141 gccgagatca aagcgatgcc gagaaaggcc cagcggaaat ccgccgccgt gacatgtgct
     1201 gtgtggtgaa tggcttccga cgtttgcagc acaaaagcgc cgatcgtaat gccgacgctc
     1261 agcgataatt gctgaacgac ggaggagaag cttgtcgccc ggctcatcgt attgtggtcg
     1321 atgtcggcat aggtgatggc gttgatcgcc gtgaactcca gtgagcgaaa gaagccgcca
     1381 aagagcagaa gccccatcat cagaaggtgc ggcgtcgtcg gcgtgaaaaa cccgtagcag
     1441 cccagaatgg tggcgctcag aaccgaattg aacaagagaa cgtgccggaa gccgaacgtg
     1501 cgcaggatag gtgcggccgt tgttttcatg gccattgcgc cgagcgacgt gatgaaggtc
     1561 atgctgccgg attggaacgc gttgaggccg aagccgatct gcagcatcaa gggcaggagc
     1621 agcggcgtcg cgccgacgcc gacgcgaaac aatgagccgc cggtgagcga ggcacggaac
     1681 gttggcactt tcaacagact gaggtcgaga atcgggaatt tcgcgcggcg cgcatgaaaa
     1741 taataggcta cgagcaagag cgtcccgaag cccatcagcg caagcacgac ctcaagactg
     1801 acgatgttac gtccgaagag cgtcatgccg aaaatcagtg tggcgagacc cgtgcccgag
     1861 agcaggaaac ccgatacgtc gagtggccaa tagtcgccgc gcaggttctt gatgtgcagg
     1921 ctgacgagta cgatcccgat cgccccgatc ggcacgttga tgaagaagat ccagcgccag
     1981 tggaagtatg tcgtgatgaa gccgccgacg agcggcccga acagcggccc gagcagggct
     2041 ggaatggtta gataggccag cgcattgatg acctccgact tcggcactgt ccgcaacaga
     2101 acgagccggc cgacgggcac catcattgcc ccgcccaccc cctggacaat ccgcgccaaa
     2161 acgaattgcc agagcgtgct ggagaagccg cagaaaatcg aacccagcgt gaagacgaca
     2221 atcgctgccc ggaagatcgt gcgggcgccg aaccggtccg ccgcccagcc gctcgccggg
     2281 atgaaaatcg ccagcgaaag cagataggaa gtcagggcca gtttgagcga aatcggatcc
     2341 tgatgcaggt cggccgcgat cgctggcagc gatgtcgaga tcaccgtgcc gtcgaggttt
     2401 tccatgaaaa gggcgcacgc gacgataaac gccgtcaggc catagcgcgg caaggcaggt
     2461 ctttcacggg atggcagcca gaatcctcgt catctaaacg cacccggctg aaattaatag
     2521 ggtggctggc tggctggggt tttccacatc ttctccctat ttctagggcg tgagcgcggc
     2581 ggcttttgtt cccgcgcctc agttttcttt gcagcacggc accggcatgc taagagccgc
     2641 tgccaacttg ggtccgccgc gcatcgctca atcgagtcgc ggccgttttt tcatctagcg
     2701 cctgcggcgc tgtcgggagt tggctatggc ggcattgcag cgtggggtca tcaaggaagc
     2761 tttgacgttt gacgacgtcc tgctcctgcc ggggcattcc gaaatcctgc cgagcaacgc
     2821 tgacctcaag acccgtctca ctcccgagat tatgctgaac ctgccaatca tttccgccgc
     2881 catggatacg gttaccgagg cgcggctcgc catcgcgctg gcgcaggccg gcggcatcgg
     2941 ggtcatccac cgcaatatgg accccgaagc gcaggccgaa gaggtccgca aggtcaagcg
     3001 ttacgaaagc ggcatggtgg tcaatcccat caccatcgtg cccgacgcga cgctcgccga
     3061 cgcgctggcg ttgatgaagc ggcacggcat ttcgggcatt ccggtcgtcg agcacgggcc
     3121 ggtggggaaa ccggcgcggc tcgtcggcat tctgaccaat cgcgacgtcc gttttgccga
     3181 caatcccaat cagccggtct ccgagctaat gaccaagaag gtcatcaccg ttacggaagg
     3241 cgtgccacag gacgaagcgc gccgcctgct ccatcaacac cgtctggaaa agctcgttgt
     3301 catcgacgag gaagggcgct gcgtcggcct tctgaccgtc aaggacatcg aaaa
//
LOCUS       DASWOG010000428         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_46092,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000428 DASWOG010000000
VERSION     DASWOG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46092"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..996)
                     /gene="mdcG"
                     /locus_tag="VGB93_08855"
     CDS             complement(358..996)
                     /gene="mdcG"
                     /locus_tag="VGB93_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HEY0146245.1"
                     /translation="MRRHDLVRVDHRAWEQVMRARPELAAEPLVRDWVSRDWPLVARR
                     RHECESGFDSESIPLGLPLPPGAGKRRLSFVFAREAVVAISPPVALASAIRVAPAHWR
                     NTLERLQEICTDLRVFGSLGWMTLTGLSYLNPGSDLDLLMPLIEATDTAKLLAGLRAL
                     DIEAPMRLDGEIVRGDGAAVNWRELDGGSGKILVKSLNGAVLVERDVFLAGT"
     gene            complement(1026..1220)
                     /locus_tag="VGB93_08860"
     CDS             complement(1026..1220)
                     /locus_tag="VGB93_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HEY0146246.1"
                     /translation="MAYLRLRHWLQNAIFAPGLDNLTQTGAVVVVWDDKSSLIDRLDA
                     ILASEDDGIDKRLHHCPVRS"
     gene            complement(1564..2592)
                     /locus_tag="VGB93_08865"
     CDS             complement(1564..2592)
                     /locus_tag="VGB93_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0146247.1"
                     /translation="MKHYVGLDVSQRETAICVVDENGKVLFEGAAKSHPGDLATLIRK
                     RAPNAERIGFETGAMASWLWHELKRIGLPVVCIDARHAKAALSVRMHKSDQNDARGLA
                     ELVRVGWYREVQVKSERSQAIRSVLVARSRLVIIRRDLENQIRSMLKEYGLLFTRAIG
                     FQFRQHVGELADSTHPLRPIIDPLLAIHVHVCAEQRKIDDEIRRLAKADETTRRLMTV
                     PGVGVVTALTFRHTIDDPSRFQKATQVGAYLGLTPRRRQSGESDLNGHVSRWGDRLLR
                     SYLYEAASVLLHRTKKWSTLKAWGTRLMRRISAKKAKVAIARKIAVILHCIWVDGTSF
                     EWGQPQAA"
     gene            3005..>3432
                     /locus_tag="VGB93_08870"
     CDS             3005..>3432
                     /locus_tag="VGB93_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY0146248.1"
                     /translation="MGLEMLTVVITALMPVFVGLGIGYFAGYKKIVDNWNISSLNVLL
                     MQFLLPITLFISIARTPGSVIRDNAALAIVIALALVVVYLPFLFVERRIFKLAPGDAA
                     VQTLTSAFPNFASIGLPLLTPFFKGEAGLPVAIAIAIGSVT"
BASE COUNT          693 a         1030 c          965 g          744 t
ORIGIN      
        1 tgcgggcgcg atcaaaacgc tttagttcca tgcgtggctg aataagattc gattggatat
       61 tgggcgcatg gaggaagttc tgctcgatga cgacgatact tccggtcttg ggcgggcatc
      121 cttcggccag ccgaccgccg atcattctcg atacgcaagc gtccatgcaa ctcggctaca
      181 tgccggttgg cggttacgct aagaccgtgc atgaacatcg gcgctgcgga tgaacgtgtt
      241 ggcatccatc atgtcgtatg gctgaggaga ctggacttcg ggcaggtcca tagcttgtcc
      301 atcaggctga gacgcgcagc ctcagcgatt gcgtgcggcg cttgagccgt gctgccgtca
      361 tgtgcccgcc agaaagacgt cgcgttccac gaggaccgcc ccattcagag acttgaccaa
      421 gatcttgcct gacccaccat ccaattcgcg ccagttcacc gccgcgccgt cgccccgaac
      481 gatttctcca tcaagtcgca taggcgcttc aatgtcgagc gcccgcaaac cggccagtaa
      541 tttcgcagta tccgtcgctt cgatgagtgg catgagaagg tcgaggtctg agccaggatt
      601 gagatagctc agacccgtca acgtcatcca gcctaggctg ccgaagacgc gtagatcggt
      661 gcaaatctct tgcagccttt ccagtgtatt tcgccaatgc gccggagcga ccctgatcgc
      721 tgaggcgagc gcgacagggg gcgagatcgc gacgacggct tctcgcgcga agacgaagga
      781 cagccggcgc ttgcctgccc ccggaggcaa gggcaaacca agtggtatgc tttcagagtc
      841 aaagccggac tcgcattcgt gtcgccgccg tgcgaccagc ggccagtccc gcgataccca
      901 gtctctgacg agaggttccg ccgcgagttc cggccgggcg cgcatcacct gctcccacgc
      961 gcgatgatcg acccgcacga gatcatggcg tcgcatgtcg catacagccg gtcggtcaag
     1021 cgaaatcatg aacgcaccgg acaatggtga agtcgcttgt cgatgccgtc atcttcgctg
     1081 gctaagatcg cgtccagtcg atcgataagg ctggacttgt cgtcccacac aaccaccacc
     1141 gctcccgtct gagtaaggtt atcgagcccc ggcgcaaaga ttgcgttttg caaccaatga
     1201 cgcaaacgca gataagccac ctttataatc tgaatttagt cggccacgcg actcgagcta
     1261 gctccctgac atcattcagt catgtcttcc ctacaagctt cgaacgggta gcctctccca
     1321 agatgtcaat tggcccttta atcgcggtct gatgtcaagc tgtctttgat atccgctgcc
     1381 ggggtcatgg tccttttcga ggtctgagcc tcatgttgat atccggatcg agcgcctcac
     1441 cgtgttgaac gaggtccggc catcatggcc ggcgccagcc gattgatcga ggtcgctgcg
     1501 acaccgtccc ggcgggacat cgcggactgc cgaggcaggc cggattatag attgaatcga
     1561 ggctcaggcg gcttgaggct gaccccattc gaaggaggtg ccgtcgaccc agatgcagtg
     1621 caggattaca gcgatcttgc gggcgatggc caccttcgcc ttcttggcgc tgatccttct
     1681 catcaggcgc gtgccccaag ccttaagcgt cgaccatttc ttggttcgat gcaacaagac
     1741 gctcgccgct tcgtaaagat agctgcggag aaggcggtcg ccccatcggg agacatgccc
     1801 gttgagatcg ctctcgcccg attgtcgacg acgcggcgtc agtccgagat aggcgccaac
     1861 ctgcgtcgcc ttttggaatc gcgacgggtc atcgatcgtg tggcggaaag tcagcgccgt
     1921 cacgacgccg acgcctggca cggtcattaa gcgtcgcgta gtctcgtcag ctttcgcaag
     1981 tcgacggatc tcatcgtcga tcttccgttg ttcggcgcac acgtgcacgt ggatcgccag
     2041 caatggatcg atgataggac gtaacgggtg agtgctatcc gccagttcgc cgacatgctg
     2101 ccgaaactga aagccaatcg cgcgcgtaaa gagcaagccg tactccttga gcatgctgcg
     2161 gatctggttc tcaagatcgc gtcgaatgat gaccaggcgc gaacgcgcga ccagcaccga
     2221 ccggatcgct tggctgcgct cgctcttaac ctgcacttct cggtaccagc caacgcgcac
     2281 gagttcggcg agcccgcgcg cgtcgttctg gtcgctcttg tgcatgcgca cggacagggc
     2341 cgcttttgcg tggcgagcgt cgatgcagac gaccggcagt cctatccgct tcaactcatg
     2401 ccacaaccaa ctggccatcg caccggtctc gaagccgatc cgctcggcgt tgggcgctcg
     2461 cttacgaatg agcgtcgcta aatcgcccgg gtgcgacttc gcggcgccct cgaataagac
     2521 cttgccgttc tcgtcgacga cgcaaatcgc tgtctcacgt tgcgagacat ccagtccaac
     2581 ataatgcttc atgacgactc ctcagattgt ggcttcagcg tacagccaac aactgtcgag
     2641 ggagaggctg acgcgattac atcagcgtgt tgaaccattt aatgccgaga cgtgtcggtg
     2701 cttgcggaat tctatctaat cgcttgtcga ccgggcagaa ctgcggcgga tcgcacgggc
     2761 tgctacgatg cagcattttt gcctgcgaaa cttcgtgcat ttccgaaaac gcgtgcgctt
     2821 ccggaagacg gcaatagcgc taggaaataa aggtttacta aagccgcaaa accggcgtcc
     2881 attcgagata tccgcccttc ggcctgcgac gcgattgacc tcgatcaatg agcgaatgat
     2941 ttacaaataa tacacttaca ttatcgttgc cgacattcgc gaacatgata attttattac
     3001 ggtcttgggg ctagaaatgc ttacagtcgt aattaccgcc cttatgcctg tattcgtagg
     3061 gttggggatc ggttacttcg ctggctataa aaaaattgtc gacaactgga acatttccag
     3121 cctgaacgtg ctgctgatgc aatttctatt gccgattact ttgttcatct cgatcgccag
     3181 gacgcccggc tccgtaatca gggacaatgc ggcgctggcc atcgtcatcg cactggctct
     3241 cgtcgtcgtc tacctgccct tcctgttcgt ggagcggcgt atcttcaagc tggctcccgg
     3301 tgatgccgcg gtgcagacct tgacctcggc ctttcccaat ttcgcgtcga tcgggctgcc
     3361 gctcctgacc cccttcttca aaggcgaagc cgggctgccg gtggccatcg ctatcgcgat
     3421 cggatcggtc ac
//
LOCUS       DASWOG010000429         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_248409,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000429 DASWOG010000000
VERSION     DASWOG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248409"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VGB93_08875"
     CDS             complement(<1..464)
                     /locus_tag="VGB93_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0146249.1"
                     /translation="MSVLPFDQREGFIWMNGVLVPWRDAKLHVLSHGLHYASSVFEGE
                     RAYDGQIFKSTEHSERFKRSANVLDFEIPYSVAELNAAKQLVVDKNGLTDCYVRPVAW
                     RGSEMMAVAAQNSTINTAIAVWAWPSMFNVEEKMKGIRLDIAEYRRPDPQTAP"
     gene            710..1273
                     /locus_tag="VGB93_08880"
     CDS             710..1273
                     /locus_tag="VGB93_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0146250.1"
                     /translation="MESLTRRKNAEPNPPIAARLTPAAQAEAEPMFDLIELLFFAYRD
                     FVSDADRLLENYGFGRAHHRVLHFVSRRPGLTIAELLDILKITKQSLNRVLKELRDEG
                     YVEVRAGAIDRRQRQLFPTKTGVNLALSIAKLQTQRFRRVFGHLQKNARPDAIAFLLA
                     MIDPREQEKVATLVASGTRLKSLGREL"
     gene            1270..2022
                     /locus_tag="VGB93_08885"
     CDS             1270..2022
                     /locus_tag="VGB93_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146251.1"
                     /translation="MTESEIEREAESGEPTIPADDAAHLLIVDDDRRIRDLLQRYLSR
                     EGYRVTLAGNAEEANAALKNLSFDLIVLDVMMPGENGFDFAARLRTDTSDRGRVPILM
                     LTAKAETDDRIAGFENGIDDFLAKPFEPRELLLRIGSILRRAQPPAPPTQITHVYFGG
                     FTFDLHRGDLRRGEDVIRLTERERDMLRLLAAKPGDTVSRELLAGSGAAGNERTVDVQ
                     VNRLRHKIERDPANPLHLQTVRGAGYRLLIDR"
     gene            2022..>2368
                     /locus_tag="VGB93_08890"
     CDS             2022..>2368
                     /locus_tag="VGB93_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEY0146252.1"
                     /translation="MTQTSASPSLQGSLAKPRALWRSVAQRLKSLMPKGLYARSLLIV
                     IFPMLILQTVVTYVFLERHWQLVTRQLSTALVQDIAAVIDVHNSYPQDKNYDTLTRIA
                     QERMNIDIEFMPKG"
BASE COUNT          478 a          771 c          670 g          449 t
ORIGIN      
        1 ggcgccgtct gcggatcggg gcggcgatat tcggcgatat cgaggcggat gcccttcatc
       61 ttctcttcga cgttgaacat gctcggccac gcccagacgg cgattgccgt attgatcgtc
      121 gaattctgtg ccgcaaccgc catcatctca ctgccgcgcc atgcaaccgg gcgcacataa
      181 caatccgtga gaccattctt atcgacgacg agctgcttgg ccgcgttcaa ctcggcgacg
      241 gaatagggaa tttcaaaatc gagcacattg gcggagcgtt tgaaccgctc cgaatgctcg
      301 gtcgatttga aaatctgccc gtcataggcg cgttcgcctt caaacacaga ggaggcataa
      361 tgcaggccgt gcgacagcac atgcagcttc gcgtcgcgcc atggaaccag aacgccgttc
      421 atccagatga aaccctcacg ctggtcaaac ggcaaaacgg acatgacgct atctccttcc
      481 gcgcgggctg agttcgcccg cagcgcattc tgactctgat cagaccgctg cgcaaaaagt
      541 ttacatgcct ttggcgagcg gaaacggcat cttgacggct atcaatcctc ttggaatatg
      601 tcaacatcac tgacttaatg cccggacctt ccggggcctg acggcgcggc gccagcgtgc
      661 tatgggccat gaaattctgc ctggaccatg caattcggga attcggctga tggaatcttt
      721 gacgcggaga aaaaatgccg aacccaaccc gccgattgcg gcgcgcctga cgcccgccgc
      781 gcaagcggaa gcggagccga tgttcgatct gatcgaactc ctgttcttcg cctatcggga
      841 ttttgtcagt gacgccgatc ggctgctcga aaactacggt tttggccgcg cacatcatcg
      901 cgtcttgcat tttgtcagcc gccgccccgg cctcaccatc gccgaactgc tcgacattct
      961 gaaaatcacc aaacagagcc tcaatcgcgt cctcaaggaa ttgcgcgacg agggctatgt
     1021 cgaggtccgc gccggcgcca tcgaccggcg gcaacgccag ctctttccca ccaagaccgg
     1081 cgtcaatctc gcgctgagca tcgcgaagct tcaaacccag cgcttccggc gcgtcttcgg
     1141 acatttgcag aaaaacgccc ggcccgacgc catcgccttc ctgctcgcca tgatcgaccc
     1201 gcgcgaacag gagaaagtcg caaccctggt ggcttcgggc acccggctca aatcgcttgg
     1261 ccgtgagcta tgaccgaatc tgagatcgaa cgcgaagccg aatccggcga gccgacgatc
     1321 ccggccgatg acgccgcgca cctgctgatc gttgacgacg accggcgcat tcgcgacctg
     1381 ttgcaacgct atctgagccg cgagggctat cgcgtcacgc tggctggcaa tgccgaggaa
     1441 gcgaatgcgg cgctcaagaa tctttcattc gatctcatcg ttctcgacgt gatgatgccc
     1501 ggcgagaacg gattcgattt cgcggcgcgg ctgcgcaccg acacatccga ccgcggccgc
     1561 gtgccgatcc tcatgctgac ggcgaaagcc gaaaccgacg accggatcgc aggttttgaa
     1621 aacgggatcg acgattttct cgccaagccc ttcgagccgc gtgaactgtt gttgcgcatc
     1681 ggctcgatcc tgcggcgggc gcagccgcca gcgccgccga cgcaaatcac gcatgtgtat
     1741 ttcggcggct tcacgttcga cctgcaccgg ggcgacctcc ggcgcggcga ggatgtcatc
     1801 cgactgaccg aacgcgagcg cgatatgttg cggctgctcg ccgcgaaacc gggcgacacg
     1861 gtgagccgtg aattgctcgc cggctccggc gccgctggga acgagcgcac cgtcgatgtc
     1921 caggtcaatc gcctgcgcca caaaatcgaa cgcgacccgg ccaatccgct gcatttgcag
     1981 accgtacgcg gcgccggcta tcgcctcctc atcgatcgct gatgacccag acgtccgcca
     2041 gcccatcgct acagggctct ctcgccaagc cgcgcgccct ctggcgcagc gtcgcacagc
     2101 ggctcaaatc gctgatgccg aaaggcctct acgcacgttc gctgctcatc gtcattttcc
     2161 cgatgctgat tctgcagacc gtggtgacct acgtctttct ggagcggcat tggcagctcg
     2221 tgacccggca gctttcgacc gcgctggttc aggacattgc cgcggtgatc gacgtccaca
     2281 attcctaccc gcaggacaag aattacgata cgctgacgcg gatcgcccag gagcgcatga
     2341 atatcgatat cgagttcatg ccgaaggg
//
LOCUS       DASWOG010000430         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_156460,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000430 DASWOG010000000
VERSION     DASWOG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156460"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1303
                     /locus_tag="VGB93_08895"
     CDS             53..1303
                     /locus_tag="VGB93_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0146253.1"
                     /translation="MKPRWTWAWFALLIAQAQFSFAETPTPPQTSLVAKGEYLARAGD
                     CIACHTTPGDKLFAGERAMLTPFGTLYTSNITPDPETGIGNWTADQFYGAMHTGRFPD
                     GGLMYPAMPFGSYTKVTRSDSDAIFAYLKTIAPVQRANRPHDLTFPYNNRSLILGWRT
                     LYFNEGTYQPDPSKSADWNRGAYLVKGLGHCAMCHTAINALGGSSDSEAFQGGLIPMQ
                     EWYAPSLTSNKEAGLGDWTIKDISDLLRTGVSSRGAVYGPMAEVVYNSLQYLSESDIR
                     AMAIYLKSLAEGSPSRTSAAAVPTDESSLLMSLGKTVYDSRCASCHGAEGAGKPPNYP
                     PLAGNQSIEMESAVNPIRMVLNGGFPPGTRGNPLPYGMPPFAQILSDDEVAAVVTYIR
                     QSWGNSGSAVSIQQANQLRSAPLN"
     gene            1498..2064
                     /locus_tag="VGB93_08900"
     CDS             1498..2064
                     /locus_tag="VGB93_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEY0146254.1"
                     /translation="MGSEPDHGTGAATAARVERRWAVVAVGIVTLMIVVAAFSGLHYT
                     IMPQARVETANPTTLHLSGEFIESNLGSAIEPGGSVTVRAIGQQFSFTPQCIVVPTDT
                     TITLRATSADVVHGLLIEGTNVNTMLVPGYISELQMRFDKPAERLMPCQEFCGIGHQG
                     MWGKVKVIDKAAFLELASKQRRLTCVDK"
     gene            2051..>3384
                     /locus_tag="VGB93_08905"
     CDS             2051..>3384
                     /locus_tag="VGB93_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0146255.1"
                     /translation="MLTNKRLILAHFSLAFALFGLALLLGAWQMFVRSPLNIWDIPPE
                     LYYRSVTAHGSVMGYVFPTLVAMGFGYAITELALEKPLIGLRWAWAGFILVAIGAVTA
                     MIPVSLGLASVLYTFYPPMIGHPLYYIGVVLVVVGSWVWVGLMSVNLAIWKRDNPGKL
                     VPLAMFANVAGSLLWGWTAVGAAIEILFMILPVALGWRSTIDAGLARVFFSWTLHAIV
                     YFWLMPAYIAYYTIFPRAIGGRVYSDPMARISFILFLVVAMPIGIHHLFADPQVGAGF
                     KFMHSVFTALVSVPTLLTIFTICASAEIAGRLRGGTGTFGWVAALPWKNPIMLATVLS
                     LVMLGFGGVGGLINMTYQLNTSIHNTQWVTGHFHLIFGGAIVIMYFAIAYDLWPHLTG
                     RALDDGGLIRTQLWLWFIGMIVTTFPWHYVGILGMPRRMAFFDYSNPLLAADA"
BASE COUNT          605 a         1048 c          958 g          773 t
ORIGIN      
        1 tgcggaagcg aaccgaactg agggccgacc tttggacagg atgtattcac tcatgaagcc
       61 gcgctggact tgggcttggt tcgcccttct gattgctcag gcgcaattca gcttcgccga
      121 gacgccgaca ccgccgcaaa catcactcgt ggcaaaaggc gaatatctcg cacgcgccgg
      181 agattgtatt gcctgccata cgacgcctgg cgacaagctg ttcgccggcg aacgcgcgat
      241 gctgacgccg tttggcactc tttatacctc gaacatcacg ccagacccgg aaactggaat
      301 cggcaattgg accgccgacc aattctatgg agcgatgcac accggccggt ttcccgacgg
      361 cggcttgatg tatcctgcga tgcccttcgg ctcttacaca aaagtcacgc gcagcgacag
      421 cgatgcgatc tttgcctatc tgaagacaat tgcgcccgtg cagcgcgcca accgaccgca
      481 cgatctgacg tttccttaca acaatcgctc gctgattctc ggatggcgta ccctctattt
      541 caacgaggga acgtatcaac ccgatccctc caaatccgcc gactggaatc gcggcgccta
      601 tcttgtcaaa ggtttggggc attgcgcgat gtgccacacc gccatcaacg cgctgggcgg
      661 tagttccgat tccgaggcct ttcagggtgg cctcattcca atgcaggagt ggtacgcccc
      721 ctcgctcacg tcgaataagg aagcgggtct cggcgactgg accatcaagg atatttcaga
      781 tctgctgcgt accggcgtgt ccagccgcgg cgccgtctat ggaccaatgg cggaggtcgt
      841 ctacaacagc ctgcaatatc tctccgaaag cgatatccgc gcgatggcca tttatctcaa
      901 gagcctcgct gaaggcagcc cgtcgcgaac ctctgcggcg gcggtgccga ccgatgaaag
      961 cagtctgctg atgagcctcg gcaagacagt ctatgattcc cgctgcgcct cctgtcacgg
     1021 cgccgagggc gcgggcaagc caccgaacta tcctccgctt gccggcaatc agtcgatcga
     1081 aatggaatcc gccgtcaacc cgatccgcat ggtgcttaat ggtggcttcc ccccgggcac
     1141 gagaggaaat cccttgccct atggtatgcc gcccttcgcg cagatcttgt ccgacgacga
     1201 ggtcgcggct gtcgtcacct atattcgtca atcctggggc aatagcggca gcgccgtctc
     1261 catccagcag gcaaaccaac tgcgttcggc tcccctcaac tgagttcatc gcatgatcaa
     1321 ttcgctccct ccccccgaga cgtcatcagc gtcagaggaa gaatgcgtcg aagccattct
     1381 gcgggcaggt cctaatggtg cgcttgtcgt cgcggcgatt gcctcgctta tcgtgctcgc
     1441 actgtggttc gcgttttact ttattgtctt cttgccgcgc gggggactgc aatgacgctg
     1501 gggtctgaac cggatcatgg caccggcgcg gccacggcgg cgcgtgtcga gcgcagatgg
     1561 gccgtggttg ctgtcggaat cgtcacgctg atgatcgtcg tcgctgcgtt ctccggcctt
     1621 cactatacaa tcatgccgca ggcgcgggtg gagacggcca atccgaccac tttgcacctc
     1681 tcgggcgagt tcatcgaaag caacctcggc agcgcgattg agcccggcgg gtccgtcacc
     1741 gtgcgcgcta tcggacaaca attctccttc acgccgcaat gcatcgtggt ccctaccgat
     1801 acgacgatca cgctacgcgc cacgagtgcc gacgtcgtgc atgggctttt gatcgaagga
     1861 acgaacgtca acacaatgct ggtacccggc tacatatccg aactccagat gcgcttcgac
     1921 aaacccgccg aacgtttgat gccttgccaa gagttctgcg ggattggcca tcagggcatg
     1981 tggggcaagg tcaaagtgat cgacaaggca gcctttctcg aactggcgtc taaacagcgg
     2041 agactgacct gtgttgacaa ataagcggct cattctcgcg catttttcgc tggcgtttgc
     2101 gctcttcggt ctcgcgttgc tgctcggcgc ctggcagatg ttcgtccgca gccctctgaa
     2161 catctgggac attccgccgg agctttatta tcgttccgtc accgcacacg gctcggtcat
     2221 gggctatgtc ttcccaacgc tcgttgcgat gggcttcggc tatgcaatca ctgaactcgc
     2281 gcttgaaaag ccgctgatcg ggttgcgctg ggcctgggct ggtttcattc ttgtggccat
     2341 cggcgccgta acggcgatga tccccgtctc tttggggctc gcctcggtgc tttacacctt
     2401 ctatccgccg atgattggcc acccgctcta ctatatcggc gtcgtcctcg tcgtggtcgg
     2461 ttcatgggtc tgggtcggcc tgatgtcggt caaccttgcg atctggaagc gcgacaatcc
     2521 cggcaagctc gtgccgctgg cgatgtttgc caatgttgcg ggatcattgc tttggggttg
     2581 gaccgcagtc ggcgctgcga ttgaaattct cttcatgatc ctgccggtcg cgcttggatg
     2641 gcgttcgacg atcgacgccg gcctcgcacg cgttttcttc tcatggacgc tgcacgccat
     2701 cgtctatttc tggctgatgc ccgcctatat cgcctattac acgatttttc cgcgcgcgat
     2761 tgggggccgg gtttacagcg atccgatggc gcgaatctcg ttcatcctgt ttctcgtcgt
     2821 cgccatgccg atcggcatac atcatctctt cgccgaccca caggtcggcg ccggtttcaa
     2881 attcatgcat tccgtgttca cggcgctcgt ttccgtgccg acgctgctga cgattttcac
     2941 gatctgcgct tcggcggaaa tcgctggccg gctgcgcggc gggaccggca cgtttggatg
     3001 ggtcgcggct ctgccgtgga aaaacccgat catgctcgcg accgtgctgt cgcttgtcat
     3061 gttggggttt ggcggcgtcg gcggcctcat caacatgacc taccagctca acaccagcat
     3121 tcataatacc caatgggtga ctggccactt ccatctgatc ttcggcggcg cgatcgtcat
     3181 catgtatttc gcgatcgctt acgacctgtg gccgcatctg acggggcgcg cgctcgacga
     3241 cggcggtttg atccgcacgc aattgtggct gtggttcatc ggcatgatcg tgacgacctt
     3301 tccctggcac tacgtcggga ttctcggaat gccgcgccga atggcctttt tcgactattc
     3361 aaatccactg ctggcggcag atgc
//
LOCUS       DASWOG010000431         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_248465,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000431 DASWOG010000000
VERSION     DASWOG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248465"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VGB93_08910"
     CDS             complement(<1..159)
                     /locus_tag="VGB93_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY0146256.1"
                     /translation="MAKFAAFLFCLIFGLGLLVSQMSNPAKVLNFLDFAGTWDPSLAF
                     VMGGAVVTT"
     gene            complement(161..583)
                     /locus_tag="VGB93_08915"
     CDS             complement(161..583)
                     /locus_tag="VGB93_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY0146257.1"
                     /translation="MTSFTPLSGLLGGVLIGLSASLLMSFNGRIAGISGILGELLAFR
                     RGAIGWRLAFIAGLVTAPLLYRSFSGALPFTITPSVPVLIVGGLLVGIGTRMGSGCTS
                     GHGVCGLARFSKRSLAATLIFMAAGIATVFVARHLIGG"
     gene            complement(627..1094)
                     /locus_tag="VGB93_08920"
     CDS             complement(627..1094)
                     /locus_tag="VGB93_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146258.1"
                     /translation="MIEFRFERLIKADAAQVFALLADLRDYDRWLPRSMAFHGTSRIS
                     EGLIRLGTTYVEESPFGIRRGIITGFIPPTTLNFEQPMQLKPAFLGEIGIKLFHALTP
                     QGDGVHVLRQLQLTPKGPVTLFAPVIARAFRTENERMMMALQSFAEAETGTRV"
     gene            complement(1236..2273)
                     /locus_tag="VGB93_08925"
     CDS             complement(1236..2273)
                     /locus_tag="VGB93_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY0146259.1"
                     /translation="MTKYKLEYIWLDGYTPVPSLRGKTQIKEFDVCPKLEQLPLWGFD
                     GSSTNQAEGHSSDCVLKPVAVYPDGARTDGALVMCEVLLPDGTAHPSNKRATILDDPG
                     AWFGFEQEYFFYKDGRPLGFPEAGYPAPQGPYYTGVGYKNVGDIARKIVEEHLDLCLF
                     AGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLLQRLTEKYGVDIEYHCKPL
                     GDTDWNGSGMHANFSTSYMREVGGKEYFEKLMKAFEINREDHIAVYGPDNHMRLTGKH
                     ETASIHEFSYGIADRGASIRVPHSFAKNDYKGYLEDRRPNSQGDPYQIASQILKTIAS
                     VGEGSAVSAAA"
     gene            2625..2879
                     /locus_tag="VGB93_08930"
     CDS             2625..2879
                     /locus_tag="VGB93_08930"
                     /inference="COORDINATES: protein motif:HMM:NF022384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2735 domain-containing protein"
                     /protein_id="HEY0146260.1"
                     /translation="MNPNSSFSSAKIYAFPAGGRATLRARLQEAHLSALGVTEAPPGS
                     GWYHDAAMREEEMARKPRSAAWWDDAESESQAKGVAVKLY"
     gene            complement(2885..3814)
                     /locus_tag="VGB93_08935"
     CDS             complement(2885..3814)
                     /locus_tag="VGB93_08935"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEY0146261.1"
                     /translation="MSQLESAVKEIAEEMRQRPDRGEVATYIPELARADPNAFGLAVV
                     DADGNVVYAGDADTPFSIQSVSKVFTLTIALGMVGDRLWNRVGREPSGSAFNSIVQLE
                     RERGIPRNPFINAGAIAVTDVILSGHQPREALGEILRFMQFLADDASITIDKAVAASE
                     NRTGFRNRALANYMKSFGVIENPVEFTLGVYFHHCAIAMSCRQLAMAGRFLAYRGRNP
                     STGLLVVQPDRARRINAIMLTCGHYDGSGEFAYHVGLPGKSGVGGGILAIAPGKASIA
                     VWSPGLDAAGNSHLGRIALEELTKRMGWSIFSA"
BASE COUNT          884 a         1210 c         1243 g          759 t
ORIGIN      
        1 cgtcgtgacg accgccccgc ccatgacgaa ggcgaggctt ggatcccagg tccccgcgaa
       61 atcgaggaag ttgaggacct tagccggatt gctcatctgc gagacgagca acccaagccc
      121 gaagatcaag cagaagagaa aagctgcgaa cttcgccact ctaacccccg ataagatgac
      181 gcgcgacgaa gaccgtcgcg attccagctg ccatgaaaat aagggtcgcg gccagcgagc
      241 gctttgagaa ccgcgcaaga ccgcagacgc catggccgct tgtgcagcct gaacccatgc
      301 gcgtgccaat cccgaccagc agcccgccga cgatgagcac aggcaccgaa ggcgtgatcg
      361 tgaaaggcag agccccggag aaggaacgat agagaagcgg cgcggtgaca aggccggcga
      421 tgaaggcgag ccgccagccg atcgcgccgc gccggaacgc gagcaattca ccaaggatgc
      481 cgctgatccc ggcgatacgg ccgttgaagg acatcagcaa cgaggccgac aggccgatta
      541 acacgccgcc gagaagcccc gagagaggtg tgaagctcgt catgcgggga atatgaggca
      601 cggcggcagt aggatcaaat cgccgcctaa acgcgggtgc cggtctccgc ttcggcaaag
      661 ctctgcaacg ccatcatcat gcgctcgttc tcagtgcgaa aggcgcgtgc gatgaccggc
      721 gcgaacagcg tcaccggccc tttcggcgtc agttggagct gacgcagcac gtgtacgccg
      781 tccccttgcg gagtcagtgc gtggaaaagc ttgatgccaa tttcaccgag aaaggccggc
      841 ttgagctgca tgggctgctc gaaattcagc gtcgtcggcg ggatgaagcc ggtgatgatc
      901 ccgcgccgga tgccgaatgg actctcttcg acataagtgg tgcccaggcg aatgagacct
      961 tcggatatgc gcgaagtccc gtgaaatgcc atcgagcgcg gaagccatcg atcataatcg
     1021 cggagatcgg caaggagcgc gaagacctgc gccgcatcag ctttgatgag cctttcaaag
     1081 cgaaactcga tcaattcttt gcctaatcga tatcttgaaa gcaaagcaga ttaacgaatg
     1141 aattaaggct caaatttcgt ctgaaacgcc caagaacctc atggcgccga agcgcgatgc
     1201 cgcggcggga ctcgatcccg ccgcggcaat aatcatcagg ccgcggccga aaccgcgctg
     1261 ccctcgccaa cagaggcgat cgtcttcagg atctgcgagg cgatctggta ggggtcgcct
     1321 tgcgagttcg gacggcgatc ctcgagatag cccttgtagt cgttcttggc gaagctgtgc
     1381 ggcacacgaa tagaggcacc gcgatccgcg atcccgtagc tgaactcgtg gatcgaagcg
     1441 gtttcgtgct tgcccgtcag acgcatgtgg ttgtccgggc catagacagc gatgtgatct
     1501 tcgcggttga tttcgaaagc tttcatgagc ttctcgaaat attccttgcc gccgacctcg
     1561 cgcatataag aggtcgagaa gttcgcgtgc atgccggagc cattccagtc ggtgtcgccg
     1621 agcggcttgc aatggtactc gatgtccaca ccgtattttt cggtgaggcg ctggagcagg
     1681 taccgcgcca tccacatctc gtcagcggcc ttcttggagc ccttgccgaa gatctggaat
     1741 tcccactggc ctttcgccac ttcggcattg atgccttcgt ggttgatgcc ggcgaagagg
     1801 caaagatcga gatgctcttc gacgatcttg cgggcgatgt cgccaacatt cttgtagccg
     1861 acgccggtgt agtagggacc ttgcggcgcc ggatagccag cttccggaaa accaagagga
     1921 cggccgtcct tatagaagaa atattcctgc tcgaatccga accacgcgcc gggatcgtcg
     1981 agaatggtcg cgcgcttgtt cgaagggtgg gcggtgccat ccggcagcaa gacttcgcac
     2041 atgacgagcg cgccatcagt ccgcgcgccg tccggataga ccgcgacggg cttcagcacg
     2101 caatctgagc tgtggccttc agcctggttc gtcgaagagc cgtcaaagcc ccatagcggc
     2161 aattgctcta gcttcgggca cacatcgaat tccttgatct gcgttttgcc gcgcagactc
     2221 ggcactggcg tatagccgtc gagccatatg tactcgagct tgtactttgt catcacagtt
     2281 tcctctttac ggcggaggtt ggaaaagccg ctatttttac cggcaggcgg ggggaatttc
     2341 tttgcctgga acgccatttt taagcatttg ctatgccagt ggcccatctc tccttgtgcc
     2401 ggctggcacc gcgcgcgcaa ggctttggaa gtctgacgtg ctcgggcgcc ctttcgccgt
     2461 gagagattgc aactcccggc gggtaagcgc gttagtgaag acgcggggca gatcgtcgtg
     2521 cctttaattg catcgatctg atcttaaatt gtgcatacgg cacaacgagg cgaaagccgg
     2581 acgggtctta aagaatcggc tcgtcctcaa gaccgagata cgccatgaac ccgaattcct
     2641 ccttctcatc ggccaagatc tacgctttcc cagcgggcgg ccgtgccacg ttgcgggcgc
     2701 ggctccagga agcccatctc agcgcccttg gggtaactga agccccgccg ggaagcggct
     2761 ggtatcacga tgcggcgatg cgcgaagaag agatggcgcg aaagccccgc tcggcggcgt
     2821 ggtgggatga cgccgaaagc gagtcgcagg ccaaaggcgt cgcggtcaag ctctactgag
     2881 gccttcaggc gctgaagatc gaccagccca ttcgcttggt taactcttcg agcgcaatcc
     2941 gacccagatg cgaattgccc gcggcatcga gacctggcga ccagacagcg atcgacgcct
     3001 tgcctggcgc aatggcgaga atgccgccac cgacaccgct cttccccggc agaccgacgt
     3061 gataggcgaa ctcgcccgaa ccgtcgtagt gcccgcatgt gagcatgatg gcgttgatgc
     3121 ggcgggcccg gtccggctga acgaccaata gacccgtcga tggattgcgg ccgcggtaag
     3181 cgagaaaccg gccggccatc gccaattgcc gacacgacat cgcgatcgcg caatggtgga
     3241 aatagacgcc aagcgtgaat tcgaccgggt tctcgatcac cccgaaagac ttcatgtaat
     3301 ttgcgagcgc gcggttgcgg aaaccggtgc gattttcaga tgccgcaacc gccttatcga
     3361 tcgtgattga tgcatcgtcg gcgagaaatt gcatgaatcg gagaatttcg cccagggcct
     3421 cgcgcggctg atgaccggac agaatgacat ccgtcaccgc gattgcgccg gcattgatga
     3481 agggattacg cgggatgccg cgttcgcgct ccaactgaac gatcgaattg aaggcactgc
     3541 ccgagggttc gcggccgacg cggttccaga gccggtctcc gaccatgccg agcgcgatcg
     3601 tcagcgtaaa aaccttggag acactctgaa tcgaaaatgg tgtatcggca tcgccggcat
     3661 agacgacatt gccgtctgca tcgaccacgg ccagtccgaa tgcattggga tcggcacgcg
     3721 cgagttccgg aatataggtc gcgacctcac cccgatcggg ccgctgccgc atttcctcgg
     3781 cgatttcctt tacggcactt tcaagttggg acatgtggaa aacccgcttc gcgattgcga
     3841 acgagccggt caattgcggc aatagcgatc caagtcaagt ctccctcccg catgtcatga
     3901 ggggacgccc gcgattgcgg cgacaagcct gttgcgccac acggccggct cggcgaaaca
     3961 taaagggtgc agcttcgaat attgctcatc gatagcgatg aaatgcgcgc gcaagcgcta
     4021 acggaaagcc tggcgcgcgc cggctttgac gaggtcccgc acgcccgcga aggcgccaat
     4081 cgcagcgtag ccgtcg
//
LOCUS       DASWOG010000432         5965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_138753,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000432 DASWOG010000000
VERSION     DASWOG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138753"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VGB93_08940"
     CDS             complement(<1..433)
                     /locus_tag="VGB93_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146262.1"
                     /translation="MLQRLPSNINGLHVLRFIPRNMSATWSLSMAYAEEEQLVRLEGA
                     LIGKSCVVRRREGEWVFDLSDDTYVSVSVPWRVVANGRIALSSEDDGQMFGRGSPLNC
                     ETEALTVFGGRIITRASVDRQTADLTLYFDDGTRIDVFNDSR"
     gene            complement(484..560)
                     /locus_tag="VGB93_08945"
     tRNA            complement(484..560)
                     /locus_tag="VGB93_08945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Met,seq:cat)
     gene            complement(666..2288)
                     /locus_tag="VGB93_08950"
     CDS             complement(666..2288)
                     /locus_tag="VGB93_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY0146263.1"
                     /translation="MFHQLLAPVAGNLGLSFLVGILPVLAVLVLLGLLRRPGWQAALA
                     GLVVGLIIAIAVWQMPPGLAVNSVVSGAVFALWPVMWIVLNALLLYNIAVESGRFDAF
                     RAWLIDHVPQDKRVVLVVIGFCFGCLLEGISGFGTPVAITSSLLILVGFPALEALVFV
                     LIFNTAPVAFGALGAPITVLGAVTGLPAHTLGAMIGRQLPVIALLLPFYVIALYGGLR
                     SLKALWPLLLVAGGSFAVSQFISSNYLDYALTDVFAALGSLVCTLLFLQVWQPAPDPE
                     FAISDAAEKRATEAQPIAPWQGWIPWLIVSAVVIIWTHFSFATVGAQAIHWPGLDKAI
                     AITLYNNKPYAAVWNFQPLATGTAILFAALITSLVIGLPINRFFACIGHTIRQAWIAV
                     VTVMLILGLAYLMNYSGMAYTLGKAVASTGHLFVFLSPFLGWVAVLLSGSDTSGNALF
                     GNLQVVAARQLNLNPILFAATNSSGGVIGKMVSPQNIATGVAVTKLKGQEGVVFARTF
                     WHSIILTVVLGILVAIQQYLIPWIIPVLPAGH"
     gene            complement(2443..3096)
                     /locus_tag="VGB93_08955"
     CDS             complement(2443..3096)
                     /locus_tag="VGB93_08955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0146264.1"
                     /translation="MHANRARAFDNAAVASAYARWAPVYDLAFSEAMRPGRKIAAAVA
                     GRRKGALVLDVGVGTGLELPMFDPSSRVVGVDLSEPMLRRAARRVADTPLDHVAGLSV
                     MDACHLAFATATFDVIVAPYVLTVLPRPEASLDEWVRVLKPGGEIVLVNHIGATGGPL
                     AWVEAKLAERSAALGWRPEFPWRVLADWLAGHDDLTLIERRGLPPFGFFTLVRIAKR"
     gene            complement(3183..4400)
                     /gene="mnmA"
                     /locus_tag="VGB93_08960"
     CDS             complement(3183..4400)
                     /gene="mnmA"
                     /locus_tag="VGB93_08960"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0146265.1"
                     /translation="MSSEPLNSLDLPKPPAETRVVVAMSGGVDSSVVAALLKEQGYDV
                     IGVTLQLYDHGAAEHRKGACCAGQDIHDARQVAARLDIPHYVLDYEQRFREKVIERFA
                     QSYVEGETPVPCVACNSDIKFADFLDTAKSLGADVLATGHYIVSRPDGAGGRALFRAE
                     DTSRDQSYFLFATTPEQLKSLRFQLGGLDKSEVRAIARRHGLLVADKADSQDICFIHS
                     GKYSNFIEQMRPDAAQPGDIVHLDGRVLGRHAGVINYTVGQRRGLGISFNDAGIAVPL
                     YVVRLDAAHARVIVGPREALATRRVTLRDLNWIGDGSLADIGPGGRDVFVRVRSTKAP
                     IAGSLHIENGEARVAFASNEDGVSPGQACVLYDGDRTDARMLGGGFICATESGLSLEA
                     RSLTDVSLAAVDG"
     gene            4781..5056
                     /locus_tag="VGB93_08965"
     CDS             4781..5056
                     /locus_tag="VGB93_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY0146266.1"
                     /translation="MVELHRPRAKYVIGPDGSPLTVADLPPTTTKRWVIRRKAEVVAA
                     VRGGLISLEEACNRYTLTVDEFLSWQMSIDQHGLAGLRTTRIQQYRM"
     gene            complement(5095..>5965)
                     /locus_tag="VGB93_08970"
     CDS             complement(5095..>5965)
                     /locus_tag="VGB93_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEY0146267.1"
                     /translation="WYDRIALMHLGVKHISWRFIALCSFTTYALAHNIGASVLSGAMV
                     RYRAYSTKGLSTAQIALLVGICSLTFMLGVLFLGGIVLVAEPLQLRRLGHLLPHALTQ
                     PETARVIGLICLVLVAAYIIGSALRLKPLVIGRLRIEYPHPDIALRQVAASSAELMGA
                     AGIIYFALPQQANPGYFVILGVFIASFSVALASESPGGLGVFELLFIKAMPAVPHDKV
                     LTALLVFRLLYLVLPLIFAGVVVVVFERRRLSEMLHADDILETASGDSRVESEKTKPE
                     SARIAKVPASHAD"
BASE COUNT         1349 a         1738 c         1833 g         1045 t
ORIGIN      
        1 ccctcgaatc gttaaataca tcgatccgag tgccatcatc gaaatagagt gtgaggtcgg
       61 cggtctgccg gtccacggaa gcgcgggtga taattcttcc accgaaaacg gtcagtgctt
      121 cggtttcgca attcaacgga gacccgcgcc caaacatctg accatcgtct tccgagctca
      181 gggcaatacg tccattagcg acgacccgcc acggcacgct aaccgagacg tatgtgtcat
      241 ccgataaatc gaaaacccac tcgccttctc tgcgtcttac gacgcagctt tttccaatca
      301 acgcaccctc aaggcgtaca agttgctctt cttccgcgta tgccatcgac aacgaccatg
      361 ttgcagacat gttgcgcgga ataaaccgca acacgtgtaa accattgata ttgctaggga
      421 gccgttgcaa cacgttgcaa catgattttg ggggtgattt tcgtctaagt ctttgatttt
      481 gtttggtagc ggaggaggga ctcgaacccc cgacacaagg attatgattc ctctgctcta
      541 gccggctgag ctactccgcc cccttgcgcg aaagcctcag aaggccgcgc cgcagcggcc
      601 tgccgtatag gccgccgctc gcgtggaagt caaggcgctg cggcgcggct gcgccgcgct
      661 cagcctcaat ggccggcggg aaggaccggg atgatccagg ggatcaaata ttgctggatt
      721 gcgaccagaa tgccgaggac gacagtcaga atgatactgt gccagaacgt acgggcaaaa
      781 acgacgcctt cctgcccttt cagcttcgta accgcgacgc cggtcgcaat attctgcggc
      841 gacaccattt tgccgatcac gccgccggac gaattggtcg cggcaaaaag gatcggattt
      901 agattgagct gccgggccgc aacaacctgc agattgccga agagcgcgtt gcccgaagtg
      961 tcgctgcccg aaagaagaac ggcgacccag ccgaggaagg gtgacaggaa cacaaacaaa
     1021 tgccctgtcg aagcgactgc cttgccgagc gtgtaggcca tgccagaata attcatgaga
     1081 taggcgaggc cgaggataag catgacggtg acgacggcaa tccaggcttg gcggatcgtg
     1141 tggccgatgc aagcgaagaa acgattgatc ggcaggccga tgaccaacga cgtgatgagc
     1201 gcggcgaaca ggatcgctgt gccggtcgcg agcggctgga agttccagac tgcggcatag
     1261 ggcttattat tatagagcgt gatcgcgatg gccttgtcga ggccgggcca atggattgcc
     1321 tgtgcgccga cagtcgcaaa gctgaaatgc gtccagatga tcacgacggc cgaaacgatg
     1381 agccagggta tccagccttg ccaaggcgca atgggctgcg cctcggtcgc ccttttctcg
     1441 gcggcgtcgg aaatcgcaaa ctccgggtcg ggtgccggct gccagacctg caggaaaagc
     1501 agcgtgcaca caagagaacc gagcgccgcg aacacgtcgg tcagcgcgta gtcgagatag
     1561 ttcgatgaga tgaattgcga gacggcaaag ctgccgcccg cgaccagcaa cagcggccaa
     1621 agcgctttca gcgaccgcag gccgccgtag agggcgatga cgtagaaagg caaaagcagg
     1681 gctatcaccg gcaattgccg tccgatcatg gcgccgagcg tgtgcgctgg taatccggtg
     1741 acggcgccga ggacggtgat tggtgcaccg agggcgccga aggcaacagg cgcggtgttg
     1801 aagatcaaaa caaagacgag ggcctcaagc gccggaaagc cgacgaggat gagcagcgag
     1861 ctggtgatgg cgaccggcgt gccgaaaccg gaaatgcctt cgagcaggca gccaaagcaa
     1921 aagccgatca cgaccaggac gacgcgcttg tcctgcggca catgatcgat aagccaggcg
     1981 cggaaggcgt cgaaacggcc tgactcgacc gcgatattgt agagaagcag cgcgttcaac
     2041 acgatccaca taaccggcca gagcgcgaag accgcgccgc tgacgacgga attgacggca
     2101 agaccgggcg gcatctgcca aacggcgatg gcgatgatca aaccgacgac aagtccggcc
     2161 agagccgcct gccaaccggg ccggcgtaaa agtccgagca aaacgagcac ggcgagcact
     2221 ggcaatatcc cgacgaggaa cgacaggcca agattccccg caacgggcgc taaaagctgg
     2281 tggaacaccg tatgtttctc cccatcgaca tcgttattct ggcttaggtc gagatgggag
     2341 agttaacttc ttccgctata cttcacaagc gcttgtcgaa atcagcattg gagagaattt
     2401 ttatactgca ttgcagcaaa tagattcgat ctagatttga tttcaccgtt tggcgatgcg
     2461 caccaaagtg aaaaagccga agggcggcag cccgcggcgc tcgattagcg tcaaatcgtc
     2521 gtggccggcg agccaatctg ccagaacgcg ccacggaaac tcagggcgcc agccgagagc
     2581 ggcgctgcgc tccgccaatt tcgcttccac ccaggcgagc ggcccccccg tcgcaccgat
     2641 gtggttgacg agaacgattt cgccgccggg tttgaggacg cgcacccatt cgtcgagact
     2701 tgcctccggc cgtggcagca cggtgagaac ataaggcgca acgatcacgt cgaaggtcgc
     2761 cgtcgcgaaa gcgaggtgac aggcatccat caccgagaga ccggcgacat ggtccagtgg
     2821 tgtgtcggcc acgcggcgcg cggcgcggcg cagcatcggc tccgaaagat cgactccgac
     2881 gacccggctc gacgggtcga acatcggcaa ttcgagacct gtcccgaccc ccacgtcgag
     2941 gacgagcgcg cccttgcggc gccctgcgac ggcggcggca atcttgcggc cgggccgcat
     3001 agcttcggaa aaagcgagat cgtaaaccgg cgcccagcgt gcgtaggcgc tggcgacagc
     3061 ggcattgtca aaagctctgg cgcgatttgc gtgcacggct acagggccgc gctggcgcgg
     3121 gcgtccggtt tggccggccg ggcgcggcgg cagaaagttg tgactcccct cgtttttgtt
     3181 catcagccgt cgacagcggc gagcgaaaca tcggtcagcg agcgcgcttc caatgacagg
     3241 ccggattcgg tcgcgcagat gaaaccgccg ccaagcatgc gggcgtcggt gcggtcaccg
     3301 tcgtagagaa cgcaagcctg tccgggagag acaccgtctt cgttgctggc gaaggccacg
     3361 cgagcctcgc cgttctcaat atgcagcgaa cctgcgatag gcgccttcgt cgagcggacg
     3421 cgcacgaaca cgtcacgacc ccctgggccg atatcggcga gcgacccgtc accaatccag
     3481 ttcagatcgc gcaaagtcac gcgtcgggtc gccaaggctt cgcggggacc gacaatgacc
     3541 cgcgcatgcg cggcgtcgag ccgcacgaca tagagcggca cggcaattcc agcatcattg
     3601 aagctgatgc cgaggccgcg gcgctggccg accgtataat tgatgacacc agcatggcgg
     3661 ccgagcacgc gcccatccag atgcacgata tcgccgggct gcgccgcatc cggacgcatc
     3721 tgctcgatga aattgctgta tttgccggaa tggatgaagc agatgtcctg gctgtcagct
     3781 ttatcggcaa caagcagacc atggcggcgc gcaattgcgc gcacctcaga cttgtcgagg
     3841 ccgccaagct gaaaccgcag gctctttaat tgttcgggcg tggttgcaaa caggaaatag
     3901 ctttgatcgc ggctcgtatc ttcggcgcgg aaaagcgcgc ggccgcccgc gccatccggg
     3961 cgcgaaacaa tgtaatgccc tgtggcgaga acgtcggcac cgagtgactt cgccgtatcg
     4021 agaaaatcgg caaacttgat atcgctgttg caggcgacgc aggggaccgg cgtctcgccc
     4081 tcgacgtagc tctgggcgaa acgctcgatg accttttcgc ggaaccgctg ctcataatcc
     4141 agaacgtagt gtggaatatc gagccgggcc gcgacttggc gcgcgtcgtg aatatcctga
     4201 ccggcgcagc aagcgccctt gcggtgttcc gcagcgccgt gatcgtaaag ctgcagcgtc
     4261 acgccgatca catcatagcc ctgctccttg agcagggctg caacgaccga ggaatcgacg
     4321 ccgcccgaca tcgcgacaac cacccgcgtc tcggcgggcg gcttcggcaa atcgaggctg
     4381 ttgagcggtt cggaactcat gcggcgatcg gatcggttcg gtaaacgtcg gcggtcatgc
     4441 cgcttatata gtcgtcttcc ggcgcgttca catgctttgc catgcgctgg cgtgggacaa
     4501 cgcgctattc taaatcaccc ctctggcagg ttggcaacgc cggtgtcgcc gtcacagcaa
     4561 gcgagaccct tgctgtcccg ctttcgggca ctcgttaagg tttcaccaag aaacgggtaa
     4621 ggtctatgta gaattcgtaa acgccatatt atcgtattca tagaaattat actaatttgc
     4681 tacccgcgag ttaagctaaa catggttatc tcgcttaggt gaatcttaag ctcgcttatt
     4741 tagtcttggg gggtgagtta gtcagtatgt gagttcagat atggtcgagt tgcaccgccc
     4801 gagggctaaa tacgtcatcg ggccagacgg gagccccctc accgtggcag atcttcctcc
     4861 gaccacgacc aaacgctggg tcatccggcg taaggctgaa gttgtggccg cagtgcgcgg
     4921 cgggctcatt tcattggaag aggcttgcaa tcgctatacg ctgacggtcg atgagttcct
     4981 gtcttggcaa atgtcgatcg accaacatgg cctcgcgggg ctgcgcacca cgcgcattca
     5041 gcaatatcgc atgtaagccc gcaataataa gcctttgtgg ccgcctccgg ccgcctaatc
     5101 ggcgtggctc gcgggcactt tggctatccg ggccgattcc ggcttggttt tttcactttc
     5161 aactcgcgaa tcaccgctgg ccgtttccag aatatcgtcg gcatgcagca tctcgctcag
     5221 gcgccgccgc tcgaagacga cgaccaccac gccggcaaag atgagtggca gaacgagata
     5281 gaggagccgg aataccaaga gtgccgtgag caccttgtca tgtggcaccg ccggcatggc
     5341 cttgatgaag agcagttcga acacgccgag cccgcctggc gattcggacg ccaaagcgac
     5401 ggaaaacgac gcgataaaaa cgccgagaat aacgaaatag ccaggattgg cctgctgtgg
     5461 cagggcaaaa tagataatgc cggcagcgcc catcaattcc gccgacgagg cggccacttg
     5521 ccgcaaggcg atgtcgggat gcggatattc aatgcgcagg cgcccgatga cgagaggctt
     5581 cagccgcagc gccgagccga tgatgtaagc cgcaacaagc acgagacaga tgaggccgat
     5641 cacccgcgcg gtttccggtt gggtcagcgc gtggggcaag agatgaccga ggcgtcgcaa
     5701 ttgtaagggt tcggcgacca gcacaatccc gccgaggaac aggacgccaa gcataaaggt
     5761 cagcgaacat atgccgacga gaagggcgat ctgcgccgtg gagagccctt tggtggaata
     5821 ggcacgataa cgcaccatgg ctcccgagag gaccgaggcc ccgatattat gggcgagtgc
     5881 ataggtcgtg aacgagcaca gcgcgatgaa gcgccaggag atgtgtttga caccgagatg
     5941 catcagcgcg attcggtcgt accag
//
LOCUS       DASWOG010000433         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_267302,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000433 DASWOG010000000
VERSION     DASWOG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267302"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGB93_08975"
     CDS             complement(<1..476)
                     /locus_tag="VGB93_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEY0146268.1"
                     /translation="MRDRLRIASWPLLTIAVGTFGGIITACLNIPLGWLIGAMVFTGA
                     LCCFYDQARVPVGLRYLGQTVIGLSAGLSLTPVLLGHALTFTPYILGGVFISIGMGLA
                     LSGFLARAAHLDATTAFFASVPGGVAEMSNLAERYGGDVAAVAIAQTLRIFMAVLTV"
     gene            complement(481..1854)
                     /locus_tag="VGB93_08980"
     CDS             complement(481..1854)
                     /locus_tag="VGB93_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0146269.1"
                     /translation="MMTDLHELSIGAVKTRFFARSLKPSDYLAALLTRLAAYDDAIHS
                     MIAIDSARANAAAFEADAAYAAGAPRGPLDGIPVAIKDVIDVAGLPTTCYSRLLLDNI
                     AARDAAAVSALRRAGAIILGKSATHEFAIGGPAFDLPFPPARNPWNVAHHPGGSSSGS
                     GAGVAAGFFPAAIGTDTGGSVRHPASACGIVGLKPTYDVISRSGVFPLAFSLDHVGSL
                     ARTVADAALLCDVMAEGRTAATHQIGQPIRGVKVGYVKHFHTADMIADTEVTEALDAA
                     AMRLVDLGAEVREITLPSLLAFAAVNRIMLQSEAIAVHGKWLRETPEAYCRLSRRSIL
                     PGVFLSAEDLIQTQRRRRQLIDAVEAAFGDVDILLTASSMETACRIDDEAEIAHTYGR
                     QARTPFNVTGHPAIALRSGMSRAGLPLSLQIAGRYHDEALICRVAAAIELPSEIPPLD
                     TTQFAAT"
     gene            complement(1844..2041)
                     /locus_tag="VGB93_08985"
     CDS             complement(1844..2041)
                     /locus_tag="VGB93_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146270.1"
                     /translation="MSASDGLEMDAESRARALGFGRAFDLFPDDVLEAAQRAANERAA
                     LAAPDDPSCEPWPPMQCPHDD"
     gene            complement(2028..2291)
                     /locus_tag="VGB93_08990"
     CDS             complement(2028..2291)
                     /locus_tag="VGB93_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146271.1"
                     /translation="MKRGTPQSIATTSTDEIRGALHELAAADAEAIVQVGTNLPMKDM
                     GVAAERWLGKPVLCINVVTYWDALRRMGITDKLYGQGLILECF"
     gene            complement(2331..2771)
                     /locus_tag="VGB93_08995"
     CDS             complement(2331..2771)
                     /locus_tag="VGB93_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146272.1"
                     /translation="MTSIYGSRGKIGIVFPSVNTVIETESAVLAPPGVTNHAARITVV
                     ERPLNTEQAFMEHVEAMRSGITGAIDQVMTCGPSHIIMGVALEAFWGGVAQADRLQHD
                     LEVHTGLGVSMGGSASVHALKAFGIRRIAILTPHLPRGDEMVRL"
     gene            complement(2795..>3344)
                     /locus_tag="VGB93_09000"
     CDS             complement(2795..>3344)
                     /locus_tag="VGB93_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0146273.1"
                     /translation="FIAHGMYQKAIQGEADESGLICAVLDKIEAFTGKRPRGWLGPGL
                     RQSLATADHLAAHGIEYTFDWNVDDIPCEMMTSGARLFAMPYTQDLNDSIIYAIERHA
                     TGEFLNRIARTLAAFETEPGPHVISMGLHPHLMGVPHRLHEMQQILDLFQSRTDVVFT
                     TPAETMDWYKIATESRTPALAQ"
BASE COUNT          694 a          972 c         1049 g          629 t
ORIGIN      
        1 acggtcagca cggccatgaa gatgcgcaac gtctgggcga ttgcgaccgc agcgacgtcg
       61 ccgccatagc gttccgccag attggacatt tcagcgacac cgcctggaac gctggcgaag
      121 aaggctgtcg tggcatctag atgtgcggcg cgtgcaagaa agcccgaaag cgcgaggccc
      181 atgccgatcg agatgaatac tccgccaagg atatagggcg tgaacgtcag tgcgtgaccc
      241 aaaagaaccg gcgtcagcga caagccggca gacagaccaa tgacggtctg cccgagataa
      301 cgcaggccaa ccggcacgcg cgcctgatcg taaaaacagc acagagcgcc ggtgaaaacc
      361 atcgcgccga tcagccagcc aagcggaatg ttcagacaag ccgtgatgat gccgccgaag
      421 gtcccgacgg caattgtcaa caacggccag gaggcgatgc gcaagcggtc tctcatgggc
      481 ttaggtcgcc gcgaattgcg tcgtatcgag cggcggaatt tccgatggta attcgatagc
      541 ggctgcgacg cggcagatga gcgcctcgtc atggtagcgt cccgcgatct gcaaggagag
      601 tggcaagccg gcgcggctca tgccgctgcg caacgcaatc gccggatgac cggtcacatt
      661 gaagggcgtg cgcgcctgac gtccataggt atgcgcaatt tcggcctcgt catcgatccg
      721 acacgcggtt tccatcgaac ttgccgtcag gagaatgtca acgtcgccga aagcagcttc
      781 aacggcgtcg atcaattggc ggcgtcgtcg ctgcgtttga atcaaatcct cggccgaaag
      841 aaaaacgccg ggcaagatgc tgcgacggct gaggcggcaa taggcctcgg gcgtttcgcg
      901 cagccatttg ccatgcacgg caatagcttc agattgaagc atgatgcgat tgacggcggc
      961 gaaggcgagc aggcttggga gggtgatctc gcgaacttcg gcgccgagat cgacaagacg
     1021 catggccgct gcatcgagcg cttcagtgac ttctgtgtcc gcaatcatat ctgcggtgtg
     1081 gaaatgtttg acgtagccga ctttcacgcc acggatcggc tggccgattt gatgcgtggc
     1141 ggcggttcgc ccttcggcca tcacgtcgca caacagcgct gcatccgcga ctgttcgggc
     1201 caggctgccg acatggtcga gcgaaaaggc gaggggaaat acgccagatc tggaaatcac
     1261 gtcgtaagtc ggcttcaatc cgacgatgcc gcaggcgctg gcgggatggc gcaccgagcc
     1321 gccggtgtcg gtgccgatcg ctgcgggaaa gaatcctgcg gcgacgcccg cgcctgaacc
     1381 ggacgacgaa ccgccgggat gatgcgcgac gttccatgga ttgcgggcgg gcggaaaagg
     1441 aagatcgaaa gcggggccgc cgatcgcaaa ttcatgcgtc gctgattttc ccaggatgat
     1501 cgcgccagcc cgtcgcaatg ccgagaccgc ggctgcgtcg cgagcagcga tattgtccag
     1561 aagtagacgt gagtaacacg ttgtcggcag accggcgaca tcgatcacgt ccttgatcgc
     1621 aacaggaatg ccgtcaagcg ggccgcgtgg cgcaccggcg gcgtaagccg catcggcctc
     1681 aaaagcggcg gcattcgcac gcgcggagtc gatagcgatc atgctgtgaa tcgcgtcgtc
     1741 atatgccgca agtcgtgtga gcagggcggc gagataatcg ctcggcttca gtgagcgcgc
     1801 gaagaaacgg gttttgactg ccccaatgct gagttcatgc aagtcagtca tcatgcgggc
     1861 attgcatcgg cggccaaggc tcgcaggatg ggtcatccgg cgccgcgagt gcggcgcgtt
     1921 cattcgccgc ccgttgtgcg gcttcaagta cgtcgtcggg aaaaaggtcg aaggcacgac
     1981 caaagccgag cgcacgggct cggctctccg cgtccatctc caagccgtca gaagcactca
     2041 aggattaatc cttgcccata aagcttgtcg gtgatgccca tgcgccgaag cgcatcccaa
     2101 taggtgacga cattgatgca cagcaccggc tttcccagcc atctttccgc ggcgacaccc
     2161 atgtctttca ttggcagatt tgtcccgact tgaacaatcg cctccgcatc tgcggcagcg
     2221 agttcgtgca gcgcgccgcg aatttcatcc gtgcttgtcg tcgcaatcga ttgtggcgtg
     2281 ccgcgcttca tcccgatgag cttgacgatg tcgaacccag cttcctcaaa ctagagccgc
     2341 accatttcgt cgccgcgcgg cagatgtggc gtcaggatcg cgatgcgccg gatgccgaaa
     2401 gccttgaggg cgtgaacgct agcgctgccg cccattgaga cgccgagccc ggtatgcact
     2461 tcaagatcgt gctgaagcct gtccgcttgg gcgacgccgc cccaaaaggc ttccagcgcg
     2521 acgcccatga tgatgtggct ggggccgcag gtcatgacct gatcaatcgc gccggtgatg
     2581 ccggagcgca tcgcttcgac atgctccata aaggcttgtt cggtattcaa tggacgctcg
     2641 acgaccgtga tacgtgcggc atggttggtg acaccaggtg gtgcgagcac ggcgctttcc
     2701 gtctcgatga cggtgttgac cgacggaaag acgattccga tctttccgcg cgagccgtag
     2761 atgctagtca tgaatctgtc ctgtccccaa gcttttactg tgcgagcgcc ggcgttcgcg
     2821 actccgttgc gatcttatac cagtccattg tttcggccgg cgtcgtgaag acaacatccg
     2881 tgcgcgactg gaagagatcg aggatctgtt gcatctcatg caggcgatgg gggacgccca
     2941 tcagatgcgg atgcagaccc attgaaatca catgcggccc cggctcggtt tcgaaagctg
     3001 cgagcgtgcg cgcaattcta ttgaggaatt cgccggtggc atggcgttcg attgcgtaga
     3061 tgatgctgtc gttcaaatcc tgcgtatagg gcatcgcgaa caggcgagca cctgatgtca
     3121 tcatctcgca tggtatgtca tcgacgttcc aatcgaacgt atattcgatg ccgtgcgcgg
     3181 cgagatgatc ggccgttgcg aggctttggc gtaggccggg gccgagccag ccacgcggcc
     3241 gtttacccgt gaaagcttcg atcttgtcga gaacagcgca aatgagccca gactcatcgg
     3301 cctcaccctg gatcgccttc tggtacatgc cgtgggcgat gaat
//
LOCUS       DASWOG010000434         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_358138,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000434 DASWOG010000000
VERSION     DASWOG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358138"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VGB93_09005"
     CDS             <1..526
                     /locus_tag="VGB93_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146274.1"
                     /translation="KYKDRLNGATSDQAQARIDTLQAAVQKLQSSVADIQPKLLHNIG
                     FRNLSAEQRATITEALKSLGNKNHYFVIDSDMSCADCASYAASFLAVFPQPPWTLQMP
                     KVLGPSDASPKGLALLTPDPSHPLPEAEAVAKALRAANIDFEIKRGAEEFFIPGGTGP
                     IETAGLLITSQAIP"
     gene            complement(553..966)
                     /locus_tag="VGB93_09010"
     CDS             complement(553..966)
                     /locus_tag="VGB93_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY0146275.1"
                     /translation="MAHWLLKSEPTSWSWQQQVEAGAKGTFWNGVRNHSAKLHLMAMK
                     VGEHAFFYHSNEGKEIVGIVTIIKPYYPDASDESGKFGMVDVRADKPLKHPVTLEAIK
                     AEPHLKNMVLVNNSRLSVQPVSEAEWKLICKMGGV"
     gene            complement(970..1989)
                     /locus_tag="VGB93_09015"
     CDS             complement(970..1989)
                     /locus_tag="VGB93_09015"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0146276.1"
                     /translation="MPERTHIAVLGAGAWGTALANLAARNAAEVHLWAHDPAHVAEMA
                     ATGVNARRLPGVPLAFNIVPTAALEDVAKADVILLAVPAQAVRETANALIEIVPPGVP
                     LIVCAKGIERATGLFLSDVMAEVLPAHPQAVLSGPSFAEDVAKTLPTAVTLAASDDAT
                     AEELAATLAAPWFRLYHSRDIRGVEIGGAAKNVLAIASGIAAGRGLGASAGAALIARG
                     FAELSRFGRAFGADVATLAGLSGLGNLVLTWSSGQSRNFALGRALGRGADVATALADG
                     KLTEGVFTSGVLVDLAHQKNIDMPICEAVDAVLAERISIDAAIEALLARPRKAEFIGA
                     RSDER"
     gene            complement(1990..>2805)
                     /gene="tsaD"
                     /locus_tag="VGB93_09020"
     CDS             complement(1990..>2805)
                     /gene="tsaD"
                     /locus_tag="VGB93_09020"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0146277.1"
                     /translation="AAAGPGLIGGILVSLTTGKALALASGNPFMAINHLEAHALTARL
                     TDVLAFPYLLLLMSGGHTQLVAVRGVGDYLRLGATVDDAVGEAFDKVAKMLGLPYPGG
                     PQVEREALKGDPTRFDFPRPMLGRPKPDFSFSGLKTAVRLEAEKIAPLNPAAVADLCA
                     SFQAAVVDTLVDRLRAGIRVFGERVGPCQAMVVAGGVAANSAIRRALMRFCGESGLRL
                     IVPPVALCTDNGAMIAWAGIERLRLGLSDDMTFAARPRWPLDSDAETLHHGKA"
BASE COUNT          550 a          881 c          864 g          510 t
ORIGIN      
        1 aaaatataaa gacagactga atggggcaac atctgatcaa gctcaagcga ggattgatac
       61 gctgcaagcc gcggtgcaga aattgcaatc gtccgtcgcg gatattcaac ccaaattatt
      121 acataacatt ggttttcgta atctctcggc ggaacaacgt gcaacgataa ccgaagctct
      181 caaatcactg ggcaacaaaa atcattactt tgttattgat agcgatatga gctgtgctga
      241 ttgcgcatcg tatgccgcga gctttctcgc cgtattccca caaccgcctt ggaccctcca
      301 aatgccgaag gtcttaggac cttcggatgc ttcgcctaag ggcttagctc tgcttacacc
      361 ggacccctcg catccattgc ccgaggcaga agcagtggcc aaggcgcttc gtgcagcgaa
      421 catcgatttc gagatcaaac gcggtgccga agaatttttc atacctggcg gaacgggtcc
      481 tatcgaaact gccggtctgc tcattacatc tcaagcgatt ccttgagaaa gggtatcatc
      541 cggcgacttg gctcaaaccc cgcccatctt gcagatgagc ttccattcgg cctcgcttac
      601 cggctgcact gacagtcgcg aattgttgac gagcaccatg tttttcagat gcggctcggc
      661 cttgatcgcc tctaacgtta ccggatgttt cagcggcttg tcggcgcgta catcgaccat
      721 gccgaacttg ccgctttcgt cggacgcgtc aggataataa ggcttgatga tcgtgacgat
      781 gccgacgatc tccttgccct cgttcgaatg atagaagaag gcgtgctcgc cgaccttcat
      841 cgccatcaga tgcagcttgg cgctgtggtt gcgcacgcca ttccagaacg tgcctttggc
      901 gccggcttcg acctgttgct gccaggacca gcttgtcggt tcgctcttca acagccagtg
      961 cgccatgtgt cacctttcgt ctgagcgcgc gccgatgaat tccgccttgc gtgggcgcgc
     1021 cagcagcgcc tcgatcgccg catcgatgct gatgcgttcg gcgagtaccg catcgacggc
     1081 ttcgcaaatc ggcatgtcga tattcttttg atgcgcgaga tcgaccagca cgccgctggt
     1141 gaagacgcct tcagtcaatt tgccgtcagc aagcgcggtc gcgacatcgg cgccgcggcc
     1201 gagcgcgcgg ccgagcgcga aattgcgcga ttgcccggag ctccaggtga gcacaagatt
     1261 gccaaggccg gagagcccgg ccagtgtcgc gacatcggcg ccgaaggccc ggccaaagcg
     1321 tgaaagttca gcaaatccgc gtgcgatcaa cgccgcgccc gcgctggcgc cgaggccgcg
     1381 ccccgcggcg atgccgctcg cgatggcgag cacgtttttc gccgcgccgc cgatttcgac
     1441 gccgcgaata tcgcgcgaat gatagagccg gaaccacggc gcggcgagcg ttgcggcaag
     1501 ctcctcggca gttgcgtcgt cgctcgcagc gagcgtcacc gcggtcggca gtgtttttgc
     1561 gacatcttcg gcgaagctcg gccccgagag cacagcctgc ggatgcgccg gcagaacttc
     1621 cgccatcaca tcgctgagaa acaggcccgt ggcgcgttct attcccttgg cgcagacgat
     1681 caacggaaca cccggcggga cgatctcgat cagcgcgttc gccgtctcgc gaaccgcctg
     1741 cgcgggcaca gcgagaagga tgacgtcggc ctttgcgaca tcttcaagcg ccgcggtcgg
     1801 cacgatgttg aacgcgaggg gcacgccagg gagacgccgt gcattgacgc ccgtcgcggc
     1861 catttcagca acatgggcag gatcatgcgc ccaaagatga acctcggctg cattgcgggc
     1921 ggcgagattg gcgagcgccg tgccccaggc gcctgcaccc agcacggcaa tatgcgttcg
     1981 ctcgggcatt caggctttgc cgtgatggag agtttcggcg tcgctatcga gcggccagcg
     2041 cggccgcgcg gcgaaggtca tgtcgtcgct gaggccgagc cgcaggcgct cgattcccgc
     2101 ccaggcgatc atcgcgccat tgtcggtgca aagcgcgacc ggcggcacga tcagccgcag
     2161 gccgctctcg ccgcaaaagc gcatcagcgc gcggcggatc gccgaattgg cagcgacgcc
     2221 tccggcgacg accatcgcct gacacggccc gacgcgctcg ccgaagacgc ggatgccggc
     2281 gcgcagccgg tccaccagcg tatcgaccac ggcggcctgg aacgaggcgc agagatcggc
     2341 gacggcggca ggattgagcg gcgcgatttt ctcagcttcg agccgtacgg cggttttcag
     2401 tcccgagaag gaaaaatcgg gtttcggccg accgagcatc gggcggggga agtcgaaacg
     2461 cgtcggatcg cctttcaggg cttcgcgctc cacttgtgga ccgccgggat agggcaggcc
     2521 gagcattttg gcgaccttgt cgaaagcttc gccgaccgcg tcatcgaccg tcgcgccgag
     2581 ccgcagataa tcgccgacgc cacgcaccgc gacgagctgc gtatgaccgc ccgacatcag
     2641 aagcagcagg tagggaaagg cgagcacgtc ggtgagccgc gcggtcagcg catgcgcctc
     2701 cagatgattg atggccatga acggattgcc ggaagccagc gccagcgcct tgccagtcgt
     2761 caggctgaca agaatgccgc cgatgaggcc aggcccggcg gcggc
//
LOCUS       DASWOG010000435         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_248700,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000435 DASWOG010000000
VERSION     DASWOG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248700"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..741
                     /locus_tag="VGB93_09025"
     CDS             211..741
                     /locus_tag="VGB93_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146278.1"
                     /translation="MSVIIEKENLLDALREAANEADRAALATIGNMLAGATWPSLQKK
                     VIETMIAAGLAVDDRFDGLVEDIKAEAQRARDAEAQKLAKRDETSAKQSATAKQNADL
                     KAQKRAEEEAAAQAEAAKAEAAAKAEAAAATANGGYAASQTRLQPSTEAPRAPRPDQP
                     LPKVDLSQGGRPQGMR"
     gene            1045..1983
                     /locus_tag="VGB93_09030"
     CDS             1045..1983
                     /locus_tag="VGB93_09030"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0146279.1"
                     /translation="MAAELALAGHDVCGIGRGAHLAAIRQDGLKLISDGRQRVARFPA
                     SDDPSDFGPQDIVICALKAHQAHASAAEFLPLLGHDTAVVTAMNGIPWWYFYKEGGQF
                     EGRHLQSVDPDAKQWRLIGPERAIGCVIDPACEVIAPGVIEHHRLNRFTIGEPDGARS
                     QRIAMLRDILISAGFDAPIREGIRWNIWLKLWGNVCFNPISLLTLATLDRIANEPGLR
                     ALCKTMMLEAKAVAEALRVRIPDAMIDRRLTAAGAVSGHKMSMLQDLERGRSIEIDAI
                     VTAVQELGRLVEVATPVVDSVLALAQERGRQSGLYC"
     gene            2850..>3057
                     /locus_tag="VGB93_09035"
     CDS             2850..>3057
                     /locus_tag="VGB93_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146280.1"
                     /translation="MRTVLSMLAAMIIAVGSLASAAEAAPIAGQAAVTKSATELGSVE
                     KTAYVWGGRTYCWYDTGWRGPGWYW"
BASE COUNT          602 a          841 c          971 g          643 t
ORIGIN      
        1 gaggaacagg gctggtttac gctattgttg acgacctcgc gcaatacgtc cggcgcttca
       61 tcgaaacccg acccctgtct gtcttgtcag gtctctcaac ccctctgttt ttcagttgcc
      121 aactcgcaaa gccgtgcaac catgagagcg ggtcacagtc gtgacctgat tcgtaaggtt
      181 gcgaatccct tagtagagtg aggtaaaaag atgagtgtca tcattgaaaa ggaaaacctg
      241 ctcgacgcgc tgcgcgaggc ggcaaacgaa gccgatcgtg cggccttggc gacgatcggc
      301 aatatgcttg ccggtgcgac ctggccgagc ctgcagaaga aagtgatcga gacgatgatc
      361 gccgcgggcc tggctgtcga cgaccgcttc gacggcttgg tcgaggacat caaggccgag
      421 gcgcaacgcg cgcgtgacgc cgaggcgcag aaactggcga agcgcgacga gaccagcgcc
      481 aagcaatctg cgaccgccaa gcaaaacgcc gacctcaagg cgcagaaacg cgccgaagag
      541 gaagcggctg cccaagctga agcggcaaaa gccgaagccg cggcaaaagc tgaggcagcg
      601 gcagcgacgg caaatggcgg atatgcggca tcgcagaccc gtctgcagcc ctcgacggag
      661 gcgccgcgtg cgccgcgccc ggatcagccc ttgccgaagg tcgacctgtc gcaaggcggg
      721 cgtccgcagg gtatgcgtta aggtccttga cgtggtccac tgaagtggtc cacttgcagg
      781 tgcgcgagag ggattgcggc gtgccgcggt tctaaaaaag ttcttgaatt tcaaatagtt
      841 gctgaggcgc tcctatttgg ctgggaatcc aggtccccca gccaacatgg tgagcgtgaa
      901 aaaggcccgt aaaacgggcc tttttgctgg gctgagcgct ccggttaaag atgctcctac
      961 cggcgcagcc aaatcatcga cgcgcatgtc ggagaagaaa acgtgagaat ttgcgtgttc
     1021 ggcgtcgggg caatcggcgg atatatggcc gccgaactgg ctctcgcggg tcatgatgtg
     1081 tgcggcatcg gccgtggcgc tcatctggcg gccatacgcc aagatggttt gaagttgatt
     1141 tcggatgggc gccagcgcgt cgcgcgcttt cccgccagcg acgacccgtc ggattttggt
     1201 ccgcaggata tcgtgatttg cgccttgaag gcgcaccagg cgcatgccag cgcggcggag
     1261 tttttaccgc tgctggggca cgacaccgcc gtcgtcaccg caatgaatgg aattccgtgg
     1321 tggtattttt ataaagaggg cggccaattt gaaggacgac acttacaaag tgtagatccg
     1381 gacgccaagc aatggcgcct gattgggcct gaacgtgcta tcggctgcgt gatcgatccg
     1441 gcctgtgagg tcatagcgcc gggtgtgatc gaacatcatc gactcaatcg cttcacgatt
     1501 ggggaacctg acggcgcccg gtcccagcgc atcgcgatgt tgcgggacat tttgatatcg
     1561 gccggttttg atgctccgat tcgcgaaggt atccgatgga acatatggct gaaactatgg
     1621 ggcaatgtct gtttcaatcc gattagcctt ttaacgttgg caacgctcga tcgaattgcc
     1681 aacgagccgg gccttcgggc tttgtgcaaa acaatgatgc tggaggcaaa agcggttgcc
     1741 gaagcgctgc gcgtgcgcat tccggacgcg atgatcgatc ggcggttgac ggctgccggt
     1801 gccgtttcag ggcacaagat gtcgatgctg caggatttag agcgcggccg gtcaatcgag
     1861 atcgacgcta tcgtcactgc ggttcaagag ctgggacgat tggtcgaggt cgcgacgccc
     1921 gtcgtcgaca gcgtcctcgc cttggcccaa gagcggggcc gtcaatctgg actctattgt
     1981 taattcttac gcgtaatatt tcgctggcgc gacgaccggg gcttcgcggc atcggcaatg
     2041 agcgataata tagcatcgta attgccaagg atcgcggcga tgagccaagc ttctgtcgcc
     2101 ctcgttttgc tcgcccgcgt atttggccca tgccaggatc cctgcgtcgt cgagtatagt
     2161 cgcgggggtc atgtcgcgtg gttcgagcaa gcggcacacg cggcccgtgc ggcgcagatg
     2221 caagtcgtta tcaacggctt ttgcggttct tcatgcatga cgctcgcgag cgaagcgcga
     2281 ccctacgtct gcattacgcc acgtgcagag tttgcgtttc atcggacgaa ttacggcgat
     2341 cccattccgc ttggacgcga tctggacgcg tggatcagga agcgcggcgg ttatcctccg
     2401 ttccgcgggg tccccggtcg catgagctac gaggcggcgc ggtcgttttg gccggcgtgt
     2461 gggccgcgag cgcctgacgg actcaacgag cggtgaaaaa ccgctgagtc cttcgcggcc
     2521 gctgtttgaa ctgaaagtgg atgtctcccg caccagccaa acgacgtcac ggttaacgtg
     2581 ggcttaaggg attgcgctcg cctcatgttt ctcgtgggga aaaatcgatt agccttagcg
     2641 ccagatgtct tgtgacgcct atgtctcatc acggtcgtat gaacacgcgc tgtttccgct
     2701 cgttgaggct gccattgcct aaatggcggt cgtttatccc gggttttctt cagttcccat
     2761 tcatcggtgc gtttctagac tcacgacggg atctcgcctt ggactgaacc gggggtggtc
     2821 tcgtcgtaaa tctcaatggg gagatcgaga tgcgtactgt tctatcgatg ctcgcggcga
     2881 tgatcatcgc tgtcggctcg ctggcgtcag cggcggaggc cgcgccgatc gccggtcagg
     2941 ctgcggttac aaaatctgcc actgaactcg gcagcgtcga aaagactgcc tatgtctggg
     3001 gcggccggac ttattgctgg tatgataccg gttggcgcgg ccccggctgg tactggt
//
LOCUS       DASWOG010000436         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_138893,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000436 DASWOG010000000
VERSION     DASWOG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138893"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..595
                     /locus_tag="VGB93_09040"
     CDS             134..595
                     /locus_tag="VGB93_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0146281.1"
                     /translation="MSKKAQEDKPEDQHARAAILARELRIVFGNLKRRLREQATAGDL
                     TLSQIAVLSRLDREGPATVSNLAREQGVRPQSMGATVAALEAAGLVRGTPDPADGRQT
                     LLSLTEACRDLIDASRIAREDRLLAVIEAKLTPAEQDELARGLKLLQRLVD"
     gene            620..1180
                     /locus_tag="VGB93_09045"
     CDS             620..1180
                     /locus_tag="VGB93_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0146282.1"
                     /translation="MPVITLDLKTALIVIDLQKGIVALPAVHPIGDVIANAAALAAAF
                     RKLGLPVVLVNVAGGAPGRNELAHSVREFPSGWTDLVPELNVQPADHKVTKHTWGAFP
                     NTDLDAFLKEAGVTQVVLAGVATSIGVESTAREAYALGFNVTIATDAVTDLNLAAHEN
                     SLTRIFPRLGETGTTREIIDLLARNA"
     gene            1183..1512
                     /locus_tag="VGB93_09050"
     CDS             1183..1512
                     /locus_tag="VGB93_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146283.1"
                     /translation="MEWAHDASYFFGGAFLANAIPHFVSGVMGKPFQSPFAKPPGEGL
                     SSSTVNVLWGFANFVFAYLLIARVGNFDLRAADDVIAIGLGVLLIGVISARLFGRFHG
                     GNSPTGA"
     gene            1517..2764
                     /locus_tag="VGB93_09055"
     CDS             1517..2764
                     /locus_tag="VGB93_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146284.1"
                     /translation="MARSTFRSLKVFNFRVWAAGSLVSNIGSWMQRTAQDWLVLTALT
                     HNNASAVGIVVALQFAPQVLLLPATGFAVDHFDKRKLIFATQTAMGLLALALGLLVVT
                     GLVRLWQVDIFAFLLGCAAAFDMPARQAFVSELVGEADLGNALALNSTSFNAARMIGP
                     AAAGLLIAAVGTGAVFLINAASFLAVLASLVFLRRDALHSRDRTERRRGGLIEGFRYV
                     WQRGDLKTILLMLLVVGMFGLNFPIFISTMAVLVFHAGAGRFGFLTAMMALGSVAGAL
                     FTARRDNPNMQVLLVSALLFGLACGLAALTPTYIVFGLVLVTVGFAVQTFTTSTNSLV
                     QLSIAPQMRGRVLAILLAIALGGTPVGAPIIGWMADRFGPRVALGVGAASGFVAAIIA
                     CVYLVRYSSLLEVRPEADKVREA"
BASE COUNT          487 a          847 c          863 g          664 t
ORIGIN      
        1 cgaacagcct atgcggtgaa tagggggctc ttccatagga ggatttctcc gccggcgcca
       61 ccgcagcatc taggaaggaa aactttacag ttaacctttg cagataccct tctcatatgc
      121 tagagtcccc cacatgagca aaaaggccca agaggataaa ccagaggacc agcatgcccg
      181 tgccgccatt ttggcgcgcg agcttcgcat cgtgttcggc aatttgaagc gccggctgcg
      241 cgagcaggcg accgctggcg atcttaccct ctcgcaaatt gcggtgctaa gccgcctcga
      301 ccgcgaaggc ccggcgaccg tctcaaatct cgcgcgcgag caaggcgtgc ggccgcaatc
      361 gatgggggca acggtcgcgg ctcttgaagc cgccggcctg gtgcgcggca cgcccgatcc
      421 cgccgacggc cgccagactc tcctgtcgct gaccgaagcc tgccgcgatt tgatcgatgc
      481 aagccggatc gcgcgcgagg atcggttgct cgccgttatc gaagcgaaat tgacgccggc
      541 tgagcaggac gaactcgccc gcggacttaa gctgctgcaa cgcctcgtcg attaaaatcc
      601 gatctctgga aggtatgaaa tgccggtcat cacgctcgat ctgaaaaccg cgctcatcgt
      661 catcgatttg caaaagggaa ttgtcgcgct gccggctgtc catccgattg gcgatgtcat
      721 cgccaacgcg gccgcgcttg ccgccgcgtt ccgaaagctc ggcttgccgg tcgtgctcgt
      781 gaacgtcgcg ggtggcgcgc cggggcgcaa tgagctggcg catagcgtgc gcgaatttcc
      841 gtccgggtgg accgatcttg ttcccgaact gaatgttcag ccggctgacc acaaagtcac
      901 caagcacacc tggggcgctt ttccgaatac cgacctcgac gcatttttga aagaagctgg
      961 cgtgacgcag gttgtgcttg cgggcgttgc aacaagtatt ggcgtcgaat cgacggcgcg
     1021 cgaagcctat gcgctaggtt tcaatgtaac gatcgcgacc gacgccgtga ccgatctcaa
     1081 cctcgccgcg cacgaaaaca gcctcacgcg catctttccg cgccttggcg agacgggtac
     1141 gacgcgggag attatcgacc tgctggcaag gaacgcttga gtatggaatg ggctcacgac
     1201 gcgtcctact tctttggcgg cgcttttctc gcgaatgcta ttccgcattt cgttagtggc
     1261 gtgatgggaa agccattcca aagcccattc gccaaaccgc cgggcgaagg tctttcatcc
     1321 tcgaccgtga acgtgctttg gggatttgcg aactttgtct tcgcctacct tctcatcgcc
     1381 cgagtaggaa acttcgatct ccgcgccgcg gatgacgtca ttgccattgg tttgggcgtt
     1441 ctgcttatcg gtgtgatatc cgcgcgcttg ttcgggcggt ttcacggcgg taattcgccc
     1501 acgggcgcct gacaacatgg ccaggagcac tttccgctca ctgaaggtct tcaactttcg
     1561 tgtctgggcc gccggctcgc ttgtctcgaa tatcgggtcg tggatgcagc gcacggccca
     1621 agactggctg gtgctgacgg cactgacgca taacaatgcg tctgccgtcg gaatcgtcgt
     1681 ggcgctccag ttcgcgccgc aggttttact gctacctgcg acaggttttg ccgtcgacca
     1741 tttcgacaaa cgcaagctca ttttcgcgac ccagacggcg atgggacttc tcgcgctggc
     1801 gctcggtctc ctcgtcgtca caggtctcgt ccggttgtgg caggtagata tttttgcgtt
     1861 tctgcttggc tgcgcggccg ccttcgatat gccggcacgc caagcctttg tttccgaact
     1921 tgtcggagag gctgacctcg gcaacgcgct tgcgttgaat tcgacgtcgt tcaacgccgc
     1981 gcggatgatc gggccggccg ccgcgggcct tctcatcgcg gccgttggca ccggcgcggt
     2041 cttcctgatc aatgctgcgt cgtttcttgc ggtgcttgct tccctcgtgt ttctgcgccg
     2101 cgacgcactt cattcacgtg atcgaaccga gcggcggcgc ggcggtctga tcgaagggtt
     2161 tcgctatgtc tggcaacgag gagatctcaa gaccattctg ctgatgctcc tggttgtggg
     2221 catgttcggg cttaattttc cgatttttat ctcaacgatg gcggtcttgg tttttcacgc
     2281 gggcgcaggc cggtttgggt ttctgaccgc gatgatggcg ttaggctccg tcgccggcgc
     2341 gctattcacg gcgcggcggg acaatccgaa tatgcaagtt ctgctcgtca gcgcgctgct
     2401 ttttggtttg gcttgcgggc tcgcggcgct gacgccgacg tatattgtct ttggcctcgt
     2461 gcttgtcacg gtgggctttg ccgtgcagac cttcacgacc tcgaccaata gcctcgttca
     2521 gctttcgatt gcaccgcaga tgcgcgggcg tgtcttggct atcctgctcg ctattgcgct
     2581 cggcggaacg ccggtgggtg caccaatcat tggctggatg gcggatcgct ttggtccgcg
     2641 tgtggcgctc ggcgtcggtg ctgcttcagg ttttgtcgcc gcgataatcg cgtgtgtcta
     2701 tctcgtcaga tattcatcgc ttctggaagt tcggcccgag gctgacaagg tcagagaagc
     2761 ctaaaatcag ctcgacgttt gggtcagttg aatttcggcc agacgcaact cggcaattgc
     2821 cgcatcgagg gtctcacgct ggcgctcgag atgacggatt t
//
LOCUS       DASWOG010000437         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_120269,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000437 DASWOG010000000
VERSION     DASWOG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120269"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGB93_09060"
     CDS             <1..251
                     /locus_tag="VGB93_09060"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HEY0146285.1"
                     /translation="RPGLSSPDSLGIYLTYDPKPGRNDGERNCISNVRNEGLSHDLAA
                     FKLAWLVDEALKRKLTGVALKDESDLLLAAEGRPAALP"
     gene            complement(310..1209)
                     /gene="truB"
                     /locus_tag="VGB93_09065"
     CDS             complement(310..1209)
                     /gene="truB"
                     /locus_tag="VGB93_09065"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0146286.1"
                     /translation="MNAPRSKRAEVNGWVVLDKPVGMTSTHAVARLKRLFNAKKAGHA
                     GTLDPLASGLLPAAFGEATKTVPFVQDGEKAYRFTIQWGAETDSDDADGQIVTRSDIR
                     PEINAILAALPHFTGTILQTPPAYSAIKINGERAYDIARDGETPELSPRSVTIHELVM
                     TESGADDATFEARCGKGTYVRAIARDLGRVLGCLGHVSALRRTRVGPFSEADAVPLAQ
                     IEDASVPDAMRRVEAGLLELPQVVVDRDSAARLRRGQSILLRGRDAPGEGAAYACCGG
                     VVVAIGAIERGELVPGRVFNLPF"
     gene            complement(1209..1613)
                     /gene="rbfA"
                     /locus_tag="VGB93_09070"
     CDS             complement(1209..1613)
                     /gene="rbfA"
                     /locus_tag="VGB93_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY0146287.1"
                     /translation="MSRVHQSGGEPSQRMLRVGEVIRHTVSSLLSRGAINDPVLDGHV
                     VTIPDVRMSPDLKLATVYVMPLGGKDMNEVLAAFDRNKKFLRGEIAHCINLKFAPDIR
                     FKPDQSFDCGARIDAILDSPQVKRDLIKDNET"
     gene            complement(1716..>3566)
                     /gene="infB"
                     /locus_tag="VGB93_09075"
     CDS             complement(1716..>3566)
                     /gene="infB"
                     /locus_tag="VGB93_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0146288.1"
                     /translation="EQDERTRSVASFRRRTQRLKGHVAEQKDKLSREVILPETITIQE
                     LASRMSERGVDVIKLLMRQGQMAKITDVIDADTAELIAEELGHTVKRVAESDVEEGLF
                     DTPDVDEHLVPRPPVVTIMGHVDHGKTSLLDAIRHKNVVSGEAGGITQHIGAYQVTAP
                     NGRLITFIDTPGHAAFTAMRARGAKVTDIVILVVAADDGVMPQTAEAIMHARAAGVPI
                     IVAINKIDKPDAKPERVRSELLQYEVQIESLGGETLAVEVSATQKLNLDKLLDLIALQ
                     AEVLDLKANPDRAAEGTVIEARLDRGRGPVATVLVQRGTLHVGDLIVGGSQWGRVRAL
                     IDDQGVTRDSAGPSTPVEILGFSGSPEAGDRVAVVETEARAREITEYREREKRDKAAA
                     RGAGARSSLVDMMSQLKTVGRKEFPLVIKGDVQGSVEAIVTTLEKLNTDEVAARVLHA
                     GVGGITESDVTLAEASNAIIIGFNVRAHKEARNLAEQAGIEIRYYNIIYNLVDDVKAA
                     MSGLLPPTLREDMLGNALILEVFNISKVGKVAGCRVTDGNVQRGANVRLIRDNVVVHE
                     GKLSTLKRFKDEVKEVVAGQECGMAFESYQDMRVGDVIECYTVQEIKRSL"
BASE COUNT          641 a         1126 c         1083 g          716 t
ORIGIN      
        1 agcggccggg cctctcgtca cccgattcgc ttggcatcta tctgacctat gatccgaagc
       61 ccggccgcaa tgacggtgag cgcaattgta tttccaacgt tcgcaatgaa ggtttgagcc
      121 atgatctggc ggcattcaag ctcgcctggc ttgtcgatga ggctttgaag cgcaagctca
      181 ccggcgtcgc gttaaaagac gagtccgatc ttttgctggc ggccgaaggc cggcccgcgg
      241 ctttgcctta aatcagagcg agatgcgaaa aagtgcatgc cggtttttcg cggagatctc
      301 tctctaactt caaaatggca ggttgaacac gcgtcccggc accaattcgc cgcgctcgat
      361 cgcgccgatc gcgaccacga cgccgccgca gcaggcatag gccgcgccct cgccgggcgc
      421 gtcgcggcca cggagcaaaa tcgattggcc gcgccgcaga cgcgcagcgg aatcgcgatc
      481 gaccacgact tgcggcaatt ccagaagccc ggcttcaacc cgccgcattg catcgggtac
      541 tgaggcatct tcgatctgag ccagaggcac agcatcggct tcgctgaagg gtccaacgcg
      601 cgtgcgccgc aaggccgaga catgaccgag acagccaagc acgcggccaa gatcgcgcgc
      661 gatagcacgc acataggtgc ccttgccgca gcgcgcctcg aaggtcgcgt catcggcgcc
      721 cgattccgtc atgaccagtt cgtggattgt cacggaccgc ggcgaaagct ccggcgtctc
      781 gccgtcgcgc gcgatgtcat aggcgcgttc gccattgatc ttgatcgccg aataggccgg
      841 cggcgtttgc aggatcgtgc cggtgaagtg gggcagcgcg gcgagaatcg cattgatctc
      901 gggccgtata tccgatcgag tgacgatctg gccgtcggcg tcatcggaat cggtctccgc
      961 accccattga atggtgaagc gatacgcctt ctcgccgtct tgcacgaaag gtacggtctt
     1021 ggttgcttcg ccgaaagcgg ccggcagaag gcccgacgcc agcggatcga gcgtgcccgc
     1081 gtggccggct ttcttggcat tgaacagtcg cttcagccgc gcgacggcat gggttgaggt
     1141 catgccgaca ggcttgtcga gcacgaccca gccattgacc tcggcgcgtt tggagcgggg
     1201 ggcgttcatc aggtctcatt atccttgata aggtcgcgtt tcacttgcgg cgaatcgagg
     1261 atcgcatcga tgcgcgcccc gcaatcgaag ctttggtccg gcttgaaacg aatgtccggc
     1321 gcgaatttca gattgatgca atgagcgatc tcgccgcgca gaaacttctt gttgcgatcg
     1381 aaggcggcga gaacctcgtt catatccttg ccgccgagcg gcatgacata gaccgtcgcg
     1441 agcttgagat cgggactcat gcgcacgtcg gggatggtga cgacatgacc atccagaacc
     1501 ggatcgttga tggcgccgcg gctaaggagg ctggagacgg tatggcggat gacttcgccc
     1561 acgcgcaaca tacgctgcga cggctcaccg ccgctctgat gtactctcga cattttcggt
     1621 aatgcctcta tccatgcgcg ccgcgaacgg cgcgcatctc gtttggttcg gggaaattat
     1681 ggcgcgatcg tttcaagccc ctcggagcgt gcgcctcaca agctccgctt gatctcctgg
     1741 acagtgtagc actcgatcac atcaccgacg cgcatgtcct ggtagctctc gaaggccatg
     1801 ccgcactcct gcccggcgac gacctctttc acttcatcct tgaaccgctt caatgtcgaa
     1861 agcttgccct cgtggacaac gacattgtct cgaatcaggc gcacattagc gccacgctgg
     1921 acgttgccgt cggtcacgcg gcagcccgcc accttgccga ccttggaaat gttgaacact
     1981 tccaggataa gcgcattgcc gagcatgtct tcgcgcagcg ttggcggcag gagaccggac
     2041 atcgccgctt tcacgtcatc tacgagattg tagatgatgt tgtaatagcg gatttcgatg
     2101 cccgcctgct cagccaggtt gcgcgcctct ttatgcgcgc ggacgttgaa gccgatgatg
     2161 atcgcgtttg acgcttccgc cagggtcacg tcggattcgg tgatgccacc gacacccgcg
     2221 tgcaacacgc gcgccgcgac ttcatcggtg ttgagcttct ccagcgtcgt aacgatcgct
     2281 tcgactgaac cttgaacgtc gcccttgatg acgagcggga attccttgcg gcccaccgtc
     2341 ttcaactgac tcatcatgtc gacaagcgag ctgcgcgcac cagcgccgcg tgccgccgcc
     2401 ttgtcgcgct tctcacgctc tcgatattcg gtgatctcac gcgcccgcgc ttccgtctca
     2461 acgacggcga cgcgatcgcc tgcttcgggc gaacccgaga aacccagaat ttcgaccgga
     2521 gtcgaaggcc cggcgctgtc ccgcgtcacg ccctggtcgt cgatcagcgc acgcacgcgg
     2581 ccccattgcg agccgccgac gataagatcg ccgacatgca acgtgccgcg ctggacgaga
     2641 acggtcgcga caggtccgcg gccgcggtcg agccgcgcct cgatcacggt cccttcggcc
     2701 gcccggtcgg ggttggcctt gaggtccaga acttcggcct gcagcgcgat cagatcgagc
     2761 agcttgtcga gattgagctt ttgggtcgcg gaaacctcga ctgcgagcgt ctcgccgccg
     2821 agcgattcaa tctgcacttc atattgcaga agttcgctgc gcacccgctc gggcttggcg
     2881 tccggcttgt cgatcttgtt gatggccaca atgatcggca cgccagcagc gcgtgcgtgc
     2941 atgatcgcct cggctgtctg cggcatgaca ccatcatccg ctgcaacgac caggatgacg
     3001 atatccgtca ctttggcgcc gcgcgcgcgc attgccgtga aggcggcgtg gccgggcgta
     3061 tcgatgaagg tgatcagccg tccgttcggc gccgtcacct gataggcgcc tatatgctgt
     3121 gtgatgccgc cggcttcgcc ggaaacgaca ttcttatgcc ggatcgcgtc gagcagcgac
     3181 gtcttgccgt ggtcgacatg gcccatgatg gtcacgacgg gcggccgcgg gaccagatgc
     3241 tcgtcaacat ccggcgtatc gaagaggcct tcctcgacat ctgattcggc gacacgcttg
     3301 accgtgtggc ccagttcctc ggcgatgagc tcggcagtat cggcgtcgat cacgtcggtg
     3361 atcttggcca tctggccctg gcgcattagc agcttgatga catcgacgcc gcgttccgac
     3421 atgcggctcg cgagttcctg gatggtaatc gtctcaggca gaatgacttc gcgcgatagc
     3481 ttgtccttct gctccgcgac atggcctttc aggcgctggg tgcggcggcg gaacgaggcc
     3541 accgaccgcg tacgctcgtc ctgttc
//
LOCUS       DASWOG010000438         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_230799,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000438 DASWOG010000000
VERSION     DASWOG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230799"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGB93_09080"
     CDS             <1..242
                     /locus_tag="VGB93_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0146289.1"
                     /translation="GWMIQIGATDDADKAHALLARAKARGLSALDDAQAFTQKVQKGE
                     STLYRARFAGLDEDDAQAVCRSLKRSGFACFAMKN"
     gene            complement(298..1023)
                     /locus_tag="VGB93_09085"
     CDS             complement(298..1023)
                     /locus_tag="VGB93_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0146290.1"
                     /translation="MPYLLVGFLVFYLVLWGMRAFARANPAALAVALRRFGGVLVLLL
                     AGLALLRGSPDGAVVLAAIGLWLLASAAKSWRSRLSTIAGRRTGATSRLRSAVIEMEV
                     DHASGTICGTVLAGPDEGKSLAQMSREQCEALHALCLREDPDGAKLLEAYLDRRFPGW
                     RPARNESADARRWRARASARPGIMTEDEAYEVLGLHKGATRDDVVRSHRSLIKKLHPD
                     HGGSTDLAARVNEAKDVLMRRHN"
     gene            complement(1122..1526)
                     /locus_tag="VGB93_09090"
     CDS             complement(1122..1526)
                     /locus_tag="VGB93_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEY0146291.1"
                     /translation="MTLHLLKLAVGAESIADLEDFQARRAQPPFHVTRMVPRRVEELL
                     DGGSLYWVIKGQIAVRQSLRAIEAFTDDDGVSRCRLVLDAATVRVRPRPFRAFQGWRY
                     LQPKDSPPDLASREVAEMPETLRNELAALGLL"
     gene            1582..>1851
                     /locus_tag="VGB93_09095"
     CDS             1582..>1851
                     /locus_tag="VGB93_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY0146292.1"
                     /translation="MDYRKKFIVAPDKRLRLSEIDPAGTADQKSETVAADLIEEYRHK
                     LAELQTLLYAENKRALLIVLQAMDAGGKDGTISHVMRCMNPQGVSV"
BASE COUNT          399 a          592 c          539 g          321 t
ORIGIN      
        1 ccggctggat gatccagatc ggtgcgaccg atgacgccga caaggcccat gccttgctgg
       61 cgcgcgccaa ggcgcgcggg ctcagcgcac tcgacgatgc gcaggccttc acacagaagg
      121 tgcagaaagg cgaatcgacg ctttatcgcg cgcgtttcgc cggcctcgat gaagacgatg
      181 cacaagccgt ctgccgcagt ctaaaaagat ctggtttcgc ctgcttcgca atgaagaatt
      241 agtggaatgt ttttatggtc gtcagcaatg acgaccgcgt cgcgtatcgc gtaggagtta
      301 gttgtgtctg cgcatcaaaa cgtccttggc ctcgttgaca cgagcagcaa gatccgtcga
      361 tcccccgtgg tcgggatgca atttctttat caaggaccgg tgcgagcgca cgacatcatc
      421 gcgcgtcgcc cccttgtgaa ggcccaggac ctcataggcc tcatcctcag tcattatgcc
      481 cggacgggca gaagcccgcg cgcgccagcg ccgcgcgtcc gcactctcgt ttcgtgccgg
      541 acgccatccg ggaaaccggc ggtccagata agcctctaaa agcttcgcgc cgtctggatc
      601 ttcgcgcaaa cacagggcat gcagcgcctc gcattgttcg cgcgacatct gcgcgagcga
      661 tttaccctcg tcgggtccgg cgagaaccgt tccacagatc gtgccggagg catggtcgac
      721 ctccatctca atgacggcgg agcgcaaccg cgacgtcgcc ccggtgcgcc gcccggcaat
      781 cgtgctcagc cggctacgcc atgatttcgc ggcgctcgcc agaagccaga gcccgatcgc
      841 ggcgaggacg acggccccat ccggactgcc acgtaaaagc gccaatcccg caaggagcaa
      901 cacgaggacg ccgccaaaac gtcgcagcgc cacggcgagc gccgccgggt ttgcacgggc
      961 aaaggcacgc atgccccaaa gcacgagata aaagaccaaa aaacctacga ggagataagg
     1021 cattcgccct gcccgtttgc gtcagagctt acgttcctat gccggcgcag tctcggcaag
     1081 cggctttgcg catgtcgaga cttttttctg gctaagaccc gttacaataa gccgagcgct
     1141 gcaagttcgt tgcgtaacgt ctccggcatc tcggcgacct cccggctcgc aagatccggc
     1201 ggggaatcct tcggctgcaa atagcgccag ccctgaaagg cccggaatgg ccgcggccgc
     1261 acccgtacgg ttgccgcatc gagcaccagc cgacagcggc tcacgccgtc gtcgtcagtg
     1321 aacgcttcga tcgcccgcag cgactgccgg acggcgatct gccctttgat aacccagtag
     1381 agcgagccgc catcgagcaa ttcttcgact cgccggggga ccatgcgcgt cacatgaaat
     1441 ggcggctgag cgcggcgtgc ttgaaaatcc tcaagatcgg caatcgactc cgcgccaacg
     1501 gcaagtttca gcagatgaag cgtcaattca gggctccggc cataatatga cggccacata
     1561 acacccgtgc tagaagccat tatggactat cgcaagaaat tcatcgtcgc accggataaa
     1621 aggcttcgcc tctcggagat cgacccagcg ggtacggcgg atcaaaaatc cgagaccgtc
     1681 gccgccgatc tgatcgagga ataccggcat aagctcgccg agctgcagac cctgctgtat
     1741 gccgaaaaca agcgggcttt gctgatcgtc ctgcaagcga tggatgccgg cggcaaggat
     1801 gggaccattt cgcatgtcat gcgatgcatg aacccacaag gcgtcagcgt c
//
LOCUS       DASWOG010000439         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_304447,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000439 DASWOG010000000
VERSION     DASWOG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304447"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..92
                     /locus_tag="VGB93_09100"
     tRNA            17..92
                     /locus_tag="VGB93_09100"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Lys,seq:ttt)
     gene            415..612
                     /locus_tag="VGB93_09105"
                     /pseudo
     CDS             415..612
                     /locus_tag="VGB93_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            837..1139
                     /locus_tag="VGB93_09110"
     CDS             837..1139
                     /locus_tag="VGB93_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146293.1"
                     /translation="MRRRARAREIIGGYFMGWQTDFKIASFQERAGIKPASPKQAEAL
                     NEISALAANLISICALEKSGIRDGDGGWHGSDPLAGAIQDIRTIFMSMDWMESSAA"
     gene            1338..1604
                     /locus_tag="VGB93_09115"
     CDS             1338..1604
                     /locus_tag="VGB93_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0146294.1"
                     /translation="MVPSIDFNAAHDNNPQAKTSNANEQQLLLTINPADWQGRPIPEK
                     QWLIPGLIPSRVVTLLSGDGGAGKTLPALQIGMAAAQIATLGET"
BASE COUNT          402 a          503 c          496 g          384 t
ORIGIN      
        1 agcgaccttc ctttgagagc gcgtagctca gccggtagag catctgactt ttaatcagga
       61 ggtctcgggt tcgatccccg acgcgctcac caataatctc aaagagttat ttagagcagg
      121 ggccggcggt aatttcgaga atcgccaccg aatcgccacc gaaaattgaa acagcgggaa
      181 cgttgcggaa cgagcgatag gcgctgttgt tgccaaatag caacacgcgg aagttccaag
      241 caacacttta ggcttttggg ttgactttag ctcttagata gcgtacaaag caagaacata
      301 aactccaaag catgtatcgt ctgcctcacc tgcgctcttt cgggcccgcg ctgctttgga
      361 gtcgttcatg cctgaaattg ctacttcctc cgggccgcat gttcgcttcg ggacgccgca
      421 cgatatgtcg gcctcagtaa gtccaccctc gacaaacttc gcatgcacga tggcggccca
      481 tcgtttttca agctggggcg tgccatcgtc tatgcgaccg ctgaccttga tacctggctc
      541 gcgagcaagc gccggtattc gacatggccc gcaaacgaca acgtgcgtca agccgagcgt
      601 ttagccgcct agaaattccc gcgcgccttg ccatagagca tccggccctt tcggtccgcg
      661 caggatatct ttgagtttcg ggtcaatttg accggaaatt aaattccgcc tcagagcgga
      721 cgaaccgccg cggccttgag cgcgcggcat aactatcgcc aagcgccggt ggaaataatg
      781 gcgcgcttct taatcgtgtc tgaaacagta cgtcgtgaaa tgcgtaacgg gcgcttgtgc
      841 gtcgccgcgc gagagcgcgc gaaatcatcg gagggtattt tatgggatgg caaacggatt
      901 tcaaaatcgc atctttccaa gagcgcgccg gaataaagcc cgcatcgcct aagcaggctg
      961 aagcgctcaa tgaaatttct gctctagcgg ctaacctcat ttcgatttgc gcactcgaaa
     1021 aatccggtat tcgcgacggc gatggcggat ggcacggcag cgacccgctt gccggcgcaa
     1081 tccaagacat tcgaacgatt ttcatgtcca tggactggat ggaatcgtcg gccgcctagt
     1141 tcagccgccg cgccgccgtc gccagaaaga acttttatcc attcacgaac gacaaacgcg
     1201 acgcggaagt agcgatatta tcccgcttgg tgttcggcgc cgacgagtgg ttctgcgttt
     1261 aatcacagat agcaggagct aaggaaagct acactcgctg tgttgctgcc cctcaacgcg
     1321 ggatcgatgg cggcgacatg gtgccttcca tcgattttaa tgcggcacac gacaacaatc
     1381 cgcaggcaaa gacgtcgaac gccaatgagc agcaactgct tctaacaatc aatcccgccg
     1441 attggcaggg ccgtcccatc cctgagaagc aatggctgat acctggattg attcccagcc
     1501 gcgtagtcac gctgctgtcg ggcgacggcg gtgccggcaa gacgctaccg gctctacaga
     1561 taggtatggc tgccgcacaa attgccacgc ttggggagac gtgaagccgg ctgccggccg
     1621 gtgtctctac attggcgccg aggacgaggc cgacgaattc catcggcgca tggcggccgt
     1681 attgacggtt ttgggtttgc attttctgat ttaagcaact tccgcctcgt accacttgcc
     1741 gaacgcgacg ctttgctgat ggttgagcaa aaagggagga tggtc
//
LOCUS       DASWOG010000440         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_194360,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000440 DASWOG010000000
VERSION     DASWOG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194360"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="rpsN"
                     /locus_tag="VGB93_09120"
     CDS             complement(<1..277)
                     /gene="rpsN"
                     /locus_tag="VGB93_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY0146295.1"
                     /translation="MAKKSSIEKNKHREKLVKQYAARRKRLKAVADDQNLPAEERFAA
                     RLKLAELPRNSSAVRVRNRCEVSGRPRGFIGKMKMSRIAVRDLGSSGQ"
     gene            complement(314..871)
                     /gene="rplE"
                     /locus_tag="VGB93_09125"
                     /pseudo
     CDS             complement(314..871)
                     /gene="rplE"
                     /locus_tag="VGB93_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390612.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(993..1310)
                     /gene="rplX"
                     /locus_tag="VGB93_09130"
     CDS             complement(993..1310)
                     /gene="rplX"
                     /locus_tag="VGB93_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0146296.1"
                     /translation="MAAKIKKGDKVVVLTGRDKGRGGEVLQVLPREERAVVRGVNIIK
                     RHQRQTPSQEGGIISKESTIHLSNLALADPKDGKATRVSFKILDDGRKVRVAKRSGDL
                     IDG"
     gene            complement(1310..1678)
                     /gene="rplN"
                     /locus_tag="VGB93_09135"
     CDS             complement(1310..1678)
                     /gene="rplN"
                     /locus_tag="VGB93_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY0146297.1"
                     /translation="MIQMQTNLDVADNSGARRVMCIKVLGGSKRKYASIGDIIVVSIK
                     EAIPRGRVKKGDVMKAVVVRTAKDIKRQDGSVIRFDSNAAVLINNQSEPVGTRIFGPV
                     PRELRAKNHMKIISLAPEVL"
     gene            complement(1770..2021)
                     /gene="rpsQ"
                     /locus_tag="VGB93_09140"
     CDS             complement(1770..2021)
                     /gene="rpsQ"
                     /locus_tag="VGB93_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY0146298.1"
                     /translation="MPKRVLQGVVVSDKQDKTVVVKVERRFTHPLLKKTVRRSKNFHA
                     NDEANKAKVGDTVLIEETRPISRLKRWVVVEGAAAATTN"
     gene            complement(2025..2234)
                     /gene="rpmC"
                     /locus_tag="VGB93_09145"
     CDS             complement(2025..2234)
                     /gene="rpmC"
                     /locus_tag="VGB93_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0146299.1"
                     /translation="MKNKQRLSDLRVMSADQLDHEVLNLKKEQFNLRFQRATGQLQDT
                     ARVRIVRRDIARAKTIAATQAKKKV"
     gene            complement(2245..2658)
                     /gene="rplP"
                     /locus_tag="VGB93_09150"
     CDS             complement(2245..2658)
                     /gene="rplP"
                     /locus_tag="VGB93_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY0146300.1"
                     /translation="MLQPKRTKFRKAFKGRIHGDSKAGYNLDFGEYGLKAMEPDRVTA
                     RQIEAARRAMTRHMKRAGRVWIRIFPDVPVSKKPTEVRMGKGKGAPEFWAVRIAPGRN
                     MFEIGGVPTELAREALTLAAAKLPIKTRFVQRIVE"
     gene            complement(2691..>3152)
                     /gene="rpsC"
                     /locus_tag="VGB93_09155"
     CDS             complement(2691..>3152)
                     /gene="rpsC"
                     /locus_tag="VGB93_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY0146301.1"
                     /translation="KAVAKLTESEVVINIVEVRKPEIDATLIADSIAQQLERRVAFRR
                     AMKRAVQSAMRLGAEGIRINCSGRLGGAEIARLEWYREGRVPLHTLRADIDYGTATAH
                     TAYGTCGIKVWVFKGEILEHDPMAQDKKMAEQDHGGGGGGERRPRRDRDAA"
BASE COUNT          551 a         1024 c          879 g          698 t
ORIGIN      
        1 tctggccact ggagccgaga tcacggactg caatacgcga catcttcatc ttgccgatga
       61 agccgcgcgg acggccggag acttcgcaac gattgcgcac gcgcaccgcc gacgaattgc
      121 gcggcaattc ggcaagcttc agccgcgcgg caaagcgctc ctcagcggga aggttctggt
      181 catccgccac agccttcagc cgcttgcggc gcgccgcata ttgcttgacg agcttctcgc
      241 gatgcttgtt cttttcgatc gaacttttct tagccatata cctacctctt ggctccgcgt
      301 ttgagaccgg gtctcactgc cggaacggga agttgaatgc gcgcaaaagg gcgcgcgcct
      361 cttcgtcggt tttcgccgtc gtgcaaacga tgacgtcgag accgagcatg ctctccgcct
      421 tgtcgtaatc gatttccggg aacacgaggt gctccttaat gccgagcgca taattgccac
      481 gcccgtcaaa gctcttcggg ttgagaccgc ggaagtcgcg cacgcggggc aacgcgatcg
      541 tcacgagacg atcgagaaac tcgtacatcc gcgtcttgcg caatgtcacc ttcgcgccga
      601 tcggcatgtt ctcgcgcacc ttgaaagtcg cgatcgcctt gcgggcccgg gtgataacag
      661 gcttctgccc cgcgatcaaa gcaagatcgg ctgccgccga ggttactttc ttcgtgtcat
      721 tgaccgcctc gccgacgccc atgttgatca cgatcttctc gatcaccggc acttaaagcg
      781 cattcttgta gccaaactgc ttgacgagct cgtcacgcac gacctcattg taatgcttct
      841 tgagacgcgg cacgtaatca gccggcgcga tgaacggctc ggcagaggcg ttttcacgcg
      901 cccgcgccga ccgggtttca ccgccgccct tggcctgctg ctttgcgtct ttcttggcgc
      961 cgcctttcgg cgcgcccttt tcgtttttcg gctcagccat cgatcaggtc tcccgaacgt
     1021 ttggcaacgc ggaccttgcg gccgtcgtcg agaattttga agctcacccg cgttgccttg
     1081 ccgtctttcg gatcggccag cgcgaggttg gagaggtgaa tcgtgctctc cttagaaatg
     1141 atgccacctt cctggctcgg cgtctggcgc tgatggcgct tgatgatatt gacgccgcgc
     1201 acaacagcac gctcttcacg cggcaatacc tgcagcacct cgccaccacg gcccttgtcg
     1261 cggccggtga gaacgacgac cttgtcgcct tttttgatct tcgcggccat cacagaacct
     1321 ccggcgcgag cgaaatgatc ttcatgtggt tcttggcgcg caactcgcgc ggcaccggtc
     1381 cgaagatgcg ggtgccgaca ggctcagact gattgttgat cagcacggcg gcattggaat
     1441 cgaagcggat gaccgaaccg tcctgccgct tgatatcctt ggcggtccgc acgaccaccg
     1501 ctttcatcac gtcgcccttc ttgacgcggc cgcgcggaat cgcctccttg atcgaaacga
     1561 caatgatgtc gccgatcgag gcatatttcc gctttgaacc gccgagcacc ttgatgcaca
     1621 tcacgcggcg cgcgccggaa ttatcggcga cgtcgagatt ggtctgcatc tgtatcatgg
     1681 ccttgcgcct ttcttgtcag acaggtttcc gtcgccggac ttcgcctgag tttcttgttt
     1741 caatgcccga atcccttgca ggacctggct taattcgttg tggccgccgc ggcaccctcg
     1801 acaacgaccc agcgtttcag ccgcgagatc ggacgagttt cctcgatcag caccgtatcg
     1861 ccgaccttgg ccttattggc ctcgtcgttc gcatgaaaat tcttcgaccg ccgcacagtc
     1921 ttcttcagca gaggatgggt gaaacgccgc tcgaccttca ccacgactgt cttatcctgc
     1981 ttgtcgctga cgacgacgcc ctggagaacg cgtttcggca tgacttaaac ctttttctta
     2041 gcttgcgtgg cggcgatcgt cttcgcccgc gcgatatcgc ggcggacaat gcgcacccgc
     2101 gccgtatcct gaagctgccc ggtcgcccgc tgaaagcgca gattaaactg ctccttcttg
     2161 aggttcagga cttcatggtc caattgatcc gcggacatga cgcgcaagtc cgacaaacgt
     2221 tgcttgtttt tcacggtgcg cctcctattc cacgatacgt tgcacgaagc gcgtcttgat
     2281 cggcagctta gcggctgcca gcgtcaacgc ttcacgcgcg agttccgtcg gcacgccgcc
     2341 gatctcgaac atgttgcggc caggcgcgat acgcacggcc cagaattccg gcgcgccctt
     2401 gcccttaccc atgcggactt ccgtcggctt tttcgacacc ggcacgtccg gaaaaatacg
     2461 gatccagacg cggccggcgc gcttcatatg ccgcgtcata gcgcggcggg cggcctcgat
     2521 ctggcgtgcg gtgacgcgat cgggttccat cgccttcagg ccatattcgc cgaagtcgag
     2581 gttgtaccca gccttcgagt cgccgtgaat acggcccttg aaagctttgc ggaacttcgt
     2641 gcgcttaggt tgcaacatgg ttctagtctt tcaaaatctt cgcgtcgttc tcaggccgcg
     2701 tcgcgatcgc gccgtggacg gcgttcaccg ccaccaccgc cgccgtgatc ctgctccgcc
     2761 atcttcttgt cctgcgccat cggatcgtgc tcgaggattt cgcccttgaa cacccagacc
     2821 ttgatgccgc acgtgccgta agccgtatgc gccgtcgcgg tgccataatc gatgtcggcg
     2881 cgcagcgtat gcagcggcac ccggccctcg cgataccatt cgagacgggc gatttcggcg
     2941 ccgccaagac ggccggagca attgatacga ataccctcag cgccaagacg catcgccgat
     3001 tgcacggcgc gcttcatcgc gcgacggaac gcaacgcggc gctcgagctg ctgggcgatc
     3061 gaatcggcga tcaatgtcgc gtcgatctcg ggcttgcgca cctcgacgat attgatgaca
     3121 acttccgact cggtgagctt cgccacggcc tt
//
LOCUS       DASWOG010000441         5989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_28947,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000441 DASWOG010000000
VERSION     DASWOG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28947"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VGB93_09160"
     CDS             <1..1047
                     /locus_tag="VGB93_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0146302.1"
                     /translation="IFAQYRRPVVLSLAAIFVAIGAYAVLKSLGHPPTKISDIEPPAV
                     VASASPPSASLAAPQAPAVAMSAPAKADDSVLSPNSIVTPPPAPPTAPPSMAGADPMA
                     TGSIGHAPAFQPSRQRDISLADLRSEARDGSALAQFALAVSYTDGRVLPRDLQTAAQW
                     YDKAASQGFAPAQYRLASFYEKGLGVARDTNKAQALYQKAAMQGNIRAMHNLAVLAAD
                     GGDSGRPDYAMAAHWFKQAADYGVRDSQYNYAVLLARGMGVSQDFVASYVWFDLAAKQ
                     GDTDSANKRDDVGARLSADQLASAKAMADAFHPRRPSASANDVALPVINTSPRPSSSE
                     APLSFGQRPKVSVL"
     gene            complement(1049..1357)
                     /locus_tag="VGB93_09165"
     CDS             complement(1049..1357)
                     /locus_tag="VGB93_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146303.1"
                     /translation="MAAAFGLCAISQAQAAASTQMIGNIGDANKASGLIRHYTLDGGH
                     RTIDAVAAVPNGGISDDVIRKVTVETAHDICADPRGAGWTLRVFLPDQTVPIATCRFR
                     "
     gene            complement(1462..1767)
                     /locus_tag="VGB93_09170"
     CDS             complement(1462..1767)
                     /locus_tag="VGB93_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146304.1"
                     /translation="MSEVELESCLGLPDQKVTEGKTTLFMYSANSSHSLSLTIPIIDF
                     VGASFSGYCHATFRFEKDRVIAVHYSGDTDDPFGGGQDALCAPIVRSCFAERASSQP"
     gene            complement(1950..2663)
                     /gene="pdeM"
                     /locus_tag="VGB93_09175"
     CDS             complement(1950..2663)
                     /gene="pdeM"
                     /locus_tag="VGB93_09175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY0146305.1"
                     /translation="MNIAIPQAGHAGSPLALHLGAASFVADTMGALYWPEERLLVVAD
                     LHLEKGSAYARRRVFLPPYDTAMTLAALSALIARYMPRTIVALGDSFHDVEGSVRLPA
                     QFCVALRQLQAGRHWVWIAGNHDPHLPEDLGGARHDELAIANIVLRHEPTPGAETFEI
                     AGHLHPVARVAGRGGSLRRRCFLSDGRRCILPAFGAYAGGLNLLDQAFAPLFAGERFA
                     HVMGRERVYKISPRNCLPD"
     gene            complement(2677..5205)
                     /locus_tag="VGB93_09180"
     CDS             complement(2677..5205)
                     /locus_tag="VGB93_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY0146306.1"
                     /translation="MATRRLKSKTKTAPEESLLPPRFAEFFARRGWTPRQHQLALLKH
                     AEAGEDVLLIAPTGAGKTLAGFLPTLIDLDRPRNRRGLHTLYLSPLKALATDVARNLD
                     IPIAEMGLNIRAETRTGDTSMSKRQRQRRDPPDILLTTPEQLALLLASADARFLFADL
                     KRVIIDELHSLTASKRGDVLSLDLARLRDIAPQMRTTGLSATVRDPDELRRWLVSQSQ
                     DRMAHLVTTPPGATPDISILDTTEHLPWSGHTTRHAIAEVYRLIKTAHMSLVFVNTRA
                     LAEFAFAELWRINEDKLPIALHHGSLDVGQRRRVEAAMSEGRLKAVVCTSTLDLGIDW
                     GDVDLVINLGAPKGASRLMQRIGRANHRLDEPSRAILVPSNRFEVLECRAAVDAAYAG
                     EQDTALARSGALDVLCQHILGMACGEPFLADDLFNEVVSAEPYRALTREEFDAALAFV
                     SHGGYALASYERFAKIKQQKDGTWRIANGRVAQTYRMNVGTIVEADMLKVRLVRGKPD
                     KGDAYTGPLRRGGRILGQIEEYFLGTLAPQDTFLFGGEILALQGIVENEALVSRAHAE
                     APKIPSYDGGKFPLSTHLAARVREMIGDPKQWHELPKPVAEWLGLQAQRSALPPADRL
                     LVETFPRGSHFHLVAYPFEGRLAHQTLGMLLTRRMERMGLKPLGFVANDYAIGVWGAA
                     DVAAAIKTKRASLAALFSEDMLGDDLEAWLLESSLMKRSFRNCAIISGMIERNLPGKE
                     KTGRQVTISTDLIYDVLRRHEPDHILLRATRQDAALGLLDLPRLAKMLARVRGRIMHK
                     ELKRVSPLAVPILLEIGRVPVYGEAREAILAEASAALTKEAAEG"
     gene            complement(5205..>5989)
                     /locus_tag="VGB93_09185"
     CDS             complement(5205..>5989)
                     /locus_tag="VGB93_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0146307.1"
                     /translation="GVPSLFATLSNMAEHGTFDLSSIRFCISGGAPLPLETRQRFEAL
                     TGCKLIEGYGLTEASPVVAANPLDAPCKNESVGPPLKGTTVEIRSLDDPMKILRAGER
                     GEICLRGLQIMAGYWRNLQETKAAFVEGALRTGDVGYLDQDGDLFLVDRIKDIIICNG
                     FKIYPRAIEEALVQHPAVDEAVAIGIADAVRGHVPKAFVTLLPGAATTPDVLLDFLKT
                     HLSRLEMPRTIEIRAMLPKTNIGKISRKDLVLEEERLHAVSG"
BASE COUNT         1150 a         1814 c         1928 g         1097 t
ORIGIN      
        1 attttcgccc aatatcggcg cccggtcgtt ctctcgctgg cggcaatctt cgtcgccatc
       61 ggcgcctacg cggttttgaa aagcctcggc cacccgccca cgaaaatctc ggacatcgag
      121 ccgccggcgg ttgttgcgag cgccagccca ccatcggcct cgcttgcggc gccgcaggcg
      181 ccggccgtag cgatgagcgc gccggccaag gccgacgaca gcgtcctgtc acccaattcc
      241 atcgtcacgc cgccgcccgc gccgccaacg gcaccgccgt ctatggccgg tgccgatccg
      301 atggcaactg gatcgattgg tcacgcgcca gcatttcagc cctcaaggca gcgcgatatc
      361 tccctcgccg acctgcgcag cgaagcgcgc gacgggagcg cgctggcgca attcgcgctt
      421 gcggtgagtt acaccgacgg ccgcgtgttg ccgcgggatc tgcaaacagc ggcgcaatgg
      481 tatgacaagg ccgcgtcgca gggctttgcc ccggcgcaat accggctcgc gtcgttctat
      541 gaaaagggcc tcggcgtcgc gcgcgatacg aataaggcgc aggcgcttta tcaaaaggcg
      601 gccatgcaag gtaatattcg cgccatgcat aatctcgcgg tgctggcggc ggacggcggt
      661 gacagcggcc gtccggatta tgcaatggcg gcgcattggt tcaaacaggc cgccgattat
      721 ggcgtccgcg acagccaata taattatgct gtgctgctgg cgcgtggcat gggcgtatcg
      781 caggacttcg ttgcctccta cgtctggttc gacctcgcgg cgaaacaggg cgatacggat
      841 tcagccaata agcgtgatga tgtcggcgcg cggctctcgg cggatcaact ggctagcgca
      901 aaggcgatgg ccgatgcttt ccatccacgc aggccgtcgg cctcagccaa cgacgtggcg
      961 ctgccagtga tcaacacaag cccgcggccg tcctcgtcgg aagcgccatt gagcttcggt
     1021 cagcgtccaa aagtatcggt gctgtagtct agcgaaaccg gcaggtcgcg atcggcaccg
     1081 tttgatcggg caggaaaacc cggagcgtcc acccagcgcc gcgcggatcg gcgcagatat
     1141 cgtgcgcggt ttccaccgtc actttgcgaa tcacatcgtc gctgatgccg ccgttgggca
     1201 cagcggccac ggcgtcgatc gtgcggtggc cgccatccag cgtgtaatgg cggataaggc
     1261 cggaggcttt gttggcgtcg ccgatattgc ctatcatctg ggtgctggcc gctgcctgcg
     1321 cctgcgaaat ggcgcagagc ccaaaagcgg cagccacaac caatctctcc ggtctaaaca
     1381 tcgcgtgttt cgccatgatt cccctcttgc gccgagtgaa tctctcatag cgaggatcgc
     1441 gggaacaggc aattcagcgc cttagggttg cgaactggcg cgctcggcaa agcacgagcg
     1501 cacgatcggc gcgcagaggg catcctggcc gccgccaaac ggatcatccg tatcgccact
     1561 gtaatggacg gcgatgacgc gatctttctc gaagcgaaag gtcgcgtgac aatagccgct
     1621 gaatgaggcg ccgacgaaat cgatgatcgg gatcgtcaag ctcaggctat gcgaggaatt
     1681 ggcgctgtac atgaagagcg tcgtcttgcc ttcggtgacc ttctggtccg gcaggccgag
     1741 acaggattcg agctccacct cggacattcc gagcaggctg gtgcgcgcct gcttggctat
     1801 ttcaacgtcg ccgcgcgagc agccggccaa cgcaaataga cacaagcata gaattccgca
     1861 tcgtttcatg acgcccccgt caaacatccg cccggggcta acggtgcagc gcagcaaaag
     1921 gttgggcggc tcgcgcgaat tttatcgagc tagtcgggca agcaattccg gggcgagatt
     1981 ttatagacgc gctcgcggcc catcacatgg gcgaaacgct cgccagcgaa gagcggcgcg
     2041 aaggcttgat cgagcaggtt gagaccgccc gcgtaggcgc cgaaagctgg caggatgcag
     2101 cgccgtccgt cgctcaaaaa gcaccgccgc cgcagcgagc cgccgcggcc ggcgacgcga
     2161 gcgaccggat gcaggtggcc ggcgatttcg aacgtctctg cgccgggcgt cggttcatgg
     2221 cggaggacga tgttggcgat ggcgagttcg tcatggcgtg cgccgccgag gtcttccggc
     2281 agatgcggat cgtgattgcc ggcgatccag acccaatgcc ggcctgcctg caattgccgt
     2341 aaggcgacgc agaattgcgc cggcagacgc acgctgcctt cgacatcgtg gaaggaatca
     2401 cccagcgcga caatcgttcg cggcatgtag cgagcgatga gcgcagaaag cgccgccagc
     2461 gtcatggccg tatcgtaggg tggcaggaag acccgtcgac gcgcgtaggc ggaccccttt
     2521 tcgaggtgca ggtcggcgac cacaagcagc cgttcctccg gccaatacag cgcgcccatc
     2581 gtgtcggcga caaagctcgc ggcgccgagg tgcagggcaa ggggtgagcc ggcgtggccc
     2641 gcctgtggaa ttgcgatatt cattttgccg ttcgtcttag ccttccgccg cctctttggt
     2701 cagcgctgcg gatgcttccg caagaatcgc ctcgcgcgcc tcgccataaa cgggcacgcg
     2761 gccgatttcg agaagaatcg gcaccgccag cggcgacacg cgtttcagct ctttatgcat
     2821 gattcggccc cggacgcgcg ccaacatttt ggcaaggcgc ggcaggtcga gcaggcccag
     2881 cgcggcatcc tgccgcgtcg cgcgcagtag aatatggtcc ggttcgtgac ggcgcaggac
     2941 gtcgtaaata agatcggtcg agatcgttac ctgccgcccg gtcttttcct tgccgggaag
     3001 attgcgttcg atcatcccgg agatgatcgc gcaattgcgg aaactacgtt tcatcagcga
     3061 ggattccagc agccatgctt cgagatcgtc gccgagcatg tcctcggaaa agagcgcggc
     3121 aagactcgcg cgcttagtct tgatcgctgc ggcgacatct gctgcccccc agacgccgat
     3181 cgcataatcg ttggcgacga aaccgagcgg tttcaagccc atgcgttcca tccgccgcgt
     3241 cagcaacatg ccgagcgtct gatgcgcgag ccggccttca aacggatagg cgacgagatg
     3301 aaagtggctg ccgcgtggaa aggtctcgac gagcagacga tccgcgggcg gcagcgccga
     3361 gcgttgcgcc tgcaagccga gccattcggc aacgggcttc ggcagctcgt gccattgctt
     3421 cggatcgccg atcatctcgc gcacgcgcgc ggcaagatgc gttgagagtg gaaacttgcc
     3481 gccatcgtag gagggaattt tcggcgcttc ggcatgcgca cgtgaaacca gcgcctcgtt
     3541 ctcgacaata ccctgcagcg ccaggatttc accgccgaat agaaacgtgt cctgcggtgc
     3601 cagtgtcccg agaaaatatt cctcgatctg gccgaggata cgaccgccgc gccgtagcgg
     3661 gccggtgtag gcatcgccct tgtccggctt gccgcgcacg aggcggactt tcagcatgtc
     3721 ggcttcgacg atggtgccga cgttcatccg ataggtctgt gcgacacggc cgttggcgat
     3781 gcgccaggtg ccatcctttt gctgcttgat tttggcgaag cgctcatagg aggcgagcgc
     3841 atacccgccg tgcgagacga aggcgagcgc cgcgtcgaat tcctcgcgcg tcaacgcacg
     3901 atagggttca gccgaaacga cttcattgaa aagatcgtcg gcgagaaacg gctcgccgca
     3961 ggccatgccg agaatatgct gacagagcac atcgagagcg ccgctgcggg ccagcgccgt
     4021 atcctgctcg ccggcatagg ccgcatcgac ggcggcgcgg cattcgagca cttcaaaacg
     4081 gttcgacggc acaaggatcg cgcgcgacgg ctcgtcgagc cgatgatttg cgcggccgat
     4141 ccgctgcatc agccggctcg cgcccttcgg cgcgccgaga ttgatgacga gatcgacgtc
     4201 gccccaatcg ataccaaggt ccagagttga ggtacagacg acggctttca gacgcccttc
     4261 cgacatcgcg gcctcgacgc ggcggcgctg gccgacatcg agcgagccgt gatgcaaggc
     4321 gatcggcagt ttgtcttcgt tgatccgcca gagttcagcg aaggcgaatt cagcaagcgc
     4381 acgcgtattg acgaagacga gactcatatg cgcggttttg atgaggcggt aaacctcggc
     4441 gatcgcgtgg cgtgtcgtat ggccggacca gggcaggtgt tcggtcgtat cgaggatcga
     4501 aatatccggc gtggcgccgg gcggcgtggt gacgagatgc gccatccgat cttgagactg
     4561 cgaaaccagc cagcggcgca attcgtctgg atcgcgcacg gtggcggaca atccggtggt
     4621 acgcatttga ggcgcgatat cacgcaggcg agcgagatcg aggctcagga catcgccgcg
     4681 cttcgacgcg gtcagcgaat gcaattcatc gatgatgacg cgtttcaaat cggcgaaaag
     4741 aaagcgcgcg tcggcgctgg caagcagaag ggcgagttgt tccggtgtcg tcaggaggat
     4801 gtccggcgga tcgcggcgtt gacgctggcg cttcgacatc gatgtgtcgc ccgtgcgtgt
     4861 ctccgcacgg atattgaggc ccatctcggc gatgggaata tcgagattgc gcgccacgtc
     4921 ggtcgcgagt gccttcagcg gtgacaggta cagcgtatgc aagccgcggc gattgcgcgg
     4981 ccggtcgaga tcgatgagcg tcggcagaaa acccgcgagc gttttgccgg cgccagtcgg
     5041 cgcgatcaag agcacgtcct cgccggcttc ggcatgtttc aaaagcgcga gctgatgttg
     5101 gcgcggcgtc cagccacgcc gcgcgaaaaa ttctgcgaag cgcggcggca gcaagctttc
     5161 ttctggggca gtcttggtct tcgatttgag gcgccgagtc gccattatcc tgaaaccgcg
     5221 tgcaaacgtt cttcctccaa aaccagatcc ttgcggctga tcttgccgat gttggttttg
     5281 gggagcattg cgcggatttc aatcgtgcgc ggcatttcga gccgtgataa atgagttttg
     5341 aggaagtcca gaagcacgtc gggcgtcgtg gcggcgccgg gtaacagcgt gacgaaagct
     5401 tttgggacgt gtccacgcac ggcgtcggca attccgatcg ccaccgcctc atcgaccgcg
     5461 ggatgctgta cgagcgcttc ttcgatcgcg cgcgggtaaa ttttgaaccc gttgcaaatg
     5521 atgatgtctt tgatccggtc gacgaggaaa aggtcgccat cctgatcgag atagccgaca
     5581 tcgccggttc gcaaggcgcc ttcgacaaag gcggccttcg tctcctggag gttgcgccaa
     5641 tagccggcca tgatctgcag cccgcgcaga cagatttcgc cgcgttcgcc ggcgcggagg
     5701 attttcatag gatcatcaag cgagcgaatt tcgacggtcg ttcctttcag cggcggtccg
     5761 acggattcat tcttgcaagg cgcatcgagc ggattggcgg cgacaacggg cgaagcttcg
     5821 gtcaggccat agccctcgat gagtttgcag ccggtcaacg cctcgaaacg ttgccgcgtt
     5881 tcaagcggca gtggcgcgcc gccggatatg caaaagcgga tcgacgacag atcgaacgtg
     5941 ccatgctctg ccatgttgct caacgttgcg aaaagactcg gcacgccgg
//
LOCUS       DASWOG010000442         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_65336,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000442 DASWOG010000000
VERSION     DASWOG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65336"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGB93_09190"
     CDS             <1..651
                     /locus_tag="VGB93_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0146308.1"
                     /translation="RDQMAEHAAKELRDGYYVNLGIGIPTLVANFIPAGVHVQLQSEN
                     GMLGMGPFPYEGEEDPDLINAGKQTITQLPTTSYFSSADSFAMIRGGHIDLAILGAME
                     VAENGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHTAKGEPKLLHHCTLPLTGS
                     GVVDLIITDLGVLAVDKAAGTGLTLIELAPGVSVDEIKQKTEAALKISPELASRAA"
     gene            779..1345
                     /locus_tag="VGB93_09195"
     CDS             779..1345
                     /locus_tag="VGB93_09195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY0146309.1"
                     /translation="MLVAVVVVALCAGAVINGKLRIPLSFTGPAAAVAVQAPPPLSTA
                     PPPAPMPAPVQANSSAEDQSEDTVEIEPNRLGQYETDIYIRGQRVHVEVDTGATFLSL
                     RYEDAVAIGLYLSASDFRYRTSTANGTGIVAKVRLDEVRIGDISIYNVDALVSQPLAM
                     RTSSLLGMSVLGRLGGVHIADGRLVLQR"
     gene            1445..2944
                     /locus_tag="VGB93_09200"
     CDS             1445..2944
                     /locus_tag="VGB93_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY0146310.1"
                     /translation="MFPKPLPELHPNSLADETTPMVKPTGFREYDARWLFEKEINLMG
                     IQALGLGLGTLLHEFGVKPEIVTGHDFRSYSSSIKLALVTGLQAAGITVHDIGLALSP
                     MAYFAQFALDVPAVAMVTASHNDNGWTGVKMGAQRPVTFGPDEMNRLKEIVLSGAYRY
                     ADGGNYRYVTDFPARYIADLTNRPKLKRKLKVVAACGNGTAGAFAPEVLEKLGCEVIP
                     LDTNLDFNFPRYNPNPEDMKMLHAMADKVREVGADLGLGFDGDGDRCGVVDNHGEEIF
                     ADKIGVMLARDLSKLYPNATFVVDVKSTGLFMTDPVLQSNGVKADYWKTGHSYIKRRV
                     TDLKALAGFEKSGHFFFNEPVGRGYDDGLLTAIHILEMLDRNPDKSMAKLYTDLPKTW
                     GSPTMSPHCDDESKYGIVDEVVATFKEMHAKGEMVAGQKIRELVTVNGVRVVTEDGTW
                     GLVRASSNKPELVVVVESPVSKARMHEMFKAVDAVLRRYPDVGAYNQTI"
BASE COUNT          625 a          936 c          887 g          622 t
ORIGIN      
        1 cgcgatcaga tggccgagca cgccgcgaaa gaattgcgcg acggttatta cgtcaatctt
       61 ggcatcggca ttccgacgct cgttgccaat tttattcccg cgggcgtgca cgtccaattg
      121 cagagcgaga acggcatgct cggcatgggt ccctttccct acgaaggcga ggaagacccc
      181 gatctcatca atgccggcaa gcagacgatc acccaattgc cgaccacgag ctatttttcg
      241 tcggccgata gtttcgcgat gatccgcggc ggccatatcg acctcgccat ccttggtgcg
      301 atggaagttg cggaaaacgg cgacctcgcc aattggatga tccccggcaa aatggtgaaa
      361 ggcatgggtg gcgcgatgga cctcgtggcc ggcgtcaaac gtgtcgtcgt cgtgatggaa
      421 catacggcca agggcgagcc gaagctgctg catcattgca ctttgccttt gaccggctcc
      481 ggcgtcgtcg atctcattat caccgacctc ggcgtccttg ccgtcgacaa ggcggccggc
      541 acggggctga cactgatcga gcttgccccc ggcgtaagcg tcgatgaaat caagcagaag
      601 accgaggcgg cgctgaagat cagcccggaa ctcgcctcgc gagcggctta gtccgttggc
      661 tgcacctata aaactgcacc tataaaacct caggttttcg cttaatcgcg ccgaaagctt
      721 tcgacggcat ggtcgcggcc atattccggg cgcgcccatg ctgcgttttg ctttatcaat
      781 gctggttgcc gtcgttgtag tcgccctgtg cgccggcgcg gtgatcaacg gcaaactgcg
      841 aatcccgcta tcatttaccg ggcccgcggc ggccgtcgcc gtgcaggcgc cgccgcccct
      901 ctcgacagcg ccgccccccg cgcctatgcc ggcaccagtt caggcgaact caagcgccga
      961 agaccaaagc gaggacacgg ttgaaatcga gccaaaccgt ctcggccaat acgagacgga
     1021 catctatatt cgcggccaaa gggttcatgt tgaggtcgac acgggagcga cctttctttc
     1081 cttgcgttac gaggacgcag tggcaatcgg actttatctt tccgcctctg actttcgtta
     1141 tcggacctcg accgccaacg gcaccggcat agttgcaaag gttcgcctcg acgaagtgcg
     1201 cattggtgat atcagtatct ataatgtcga cgccctagtt tcgcagcctt tggcgatgcg
     1261 caccagtagc cttctcggga tgagcgtttt aggccgcctc ggcggcgtcc atattgcgga
     1321 cggcaggctg gtcctccagc gctaagcgcg ctagttacat cctcaatttc gcccggctcg
     1381 cctgcttctt gcctttccgg gcgcgcaggg ccagcgctcg gcgccgcgaa accaaggatt
     1441 ctcgatgttt cccaagccct tgcccgaact gcacccgaac agcctcgccg acgaaacgac
     1501 gcccatggtg aagcccacgg gtttccgcga atacgatgcg cgctggctgt tcgagaaaga
     1561 aatcaatctc atgggcatcc aagcgcttgg ccttggcctt ggcacgctgc tgcacgaatt
     1621 tggcgtaaag cccgagatcg tcaccggcca cgatttccgc agctattcgt cgtcgatcaa
     1681 gctcgcactg gtgacgggcc ttcaggcggc cggcattaca gtccatgaca tcggccttgc
     1741 gctctcgccg atggcttatt tcgcgcaatt cgcgctggac gttcccgccg tcgcgatggt
     1801 caccgcctcc cacaacgaca acggctggac aggtgtcaaa atgggcgcgc aacgccccgt
     1861 cacctttggc cccgatgaga tgaacaggct gaaggagatc gtgctcagcg gcgcctatcg
     1921 ttatgccgac ggcggcaatt atcgttatgt gaccgatttt ccggcgcgct acatcgccga
     1981 tttgaccaat cggccgaagc tcaagcgcaa gctcaaagtc gtcgccgcct gcggcaatgg
     2041 tacggcgggc gctttcgcgc cggaggttct ggagaagctc ggctgcgaag tcatccctct
     2101 cgacaccaat ctcgacttca acttcccgcg ctacaatccc aacccggaag acatgaagat
     2161 gctgcacgcg atggccgata aggtccgaga ggtcggcgcc gatcttgggc tcggatttga
     2221 tggcgatggc gatcgatgcg gcgtcgtcga caatcacggc gaagaaattt tcgccgacaa
     2281 gatcggcgtc atgctggcgc gggatctgtc caagctctat ccaaacgcaa cgttcgtcgt
     2341 cgatgtgaaa tcgacggggc ttttcatgac cgacccggtg cttcagtcga atggcgtgaa
     2401 ggcggattat tggaagaccg gccattccta tatcaaacgc cgcgtgaccg acctcaaggc
     2461 gctcgctggc tttgagaagt cgggtcactt tttctttaac gaaccggtcg gccggggcta
     2521 cgacgacggc ctgctgacgg ccatccacat cctcgaaatg ctcgatcgca atcccgataa
     2581 gtcgatggcc aagctttata ccgatctgcc caagacgtgg ggatcgccga cgatgtcgcc
     2641 gcattgcgac gacgaaagca aatacggcat cgtcgatgag gtcgtcgcga cattcaaaga
     2701 gatgcatgcg aaaggcgaaa tggtcgccgg ccagaaaatt cgtgagctcg tcaccgtgaa
     2761 cggcgtgcgc gtcgtaacgg aagacggtac ctggggcctc gtgcgcgcgt catccaacaa
     2821 gcccgagctt gtcgtcgtcg tcgagagccc ggtatcaaaa gcccggatgc atgaaatgtt
     2881 caaagcggta gacgcggttt tacggcgtta tccggacgtc ggcgcctata accagacgat
     2941 ctaacctctt ccagcgcgct tacttgccga gcgcgctgga gctatgcgac tgaatttgcg
     3001 aggtcacatc gccgaaaaat ttgctgtcat cgccaagcac ggcggtcggc tggcagcgcg
     3061 gctcgcaagt
//
LOCUS       DASWOG010000443         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_120398,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000443 DASWOG010000000
VERSION     DASWOG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120398"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGB93_09205"
     CDS             <1..324
                     /locus_tag="VGB93_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0146311.1"
                     /translation="ERAKRVELGMPEAIAMRHRLPHFTHVFAGDGYSSGYYSYLWSEV
                     LDADGFAAFAEAGDIFDPEVAARLKQYVYSAGNRADPDAAYLSFRGRPAAAEPLLKKR
                     GLIEA"
     gene            complement(321..998)
                     /locus_tag="VGB93_09210"
     CDS             complement(321..998)
                     /locus_tag="VGB93_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0146312.1"
                     /translation="MLFAPIQWGLLTAAPRRSQVLPRLFYTIVLWLVKVRVKASGWKP
                     GKIAALVVTNHVSWTDIPALGALFPAAFVAKSEVGRWPIIRTFAQLVNTIFVDRTARR
                     TIPATNAAMLAAIANGAHVVLFPEATTHADGPHLFHSAHFAVVEDLQKLGQTYAIQPI
                     AIRYSAPYAPWIGDDALLPHVIALMKNPPLTCELIFCEPIVLTAPLTRRAVAQECFDR
                     IAAAYRF"
     gene            1250..>1762
                     /locus_tag="VGB93_09215"
     CDS             1250..>1762
                     /locus_tag="VGB93_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0146313.1"
                     /translation="MRAKRAAPVDVPRVLGRFGNFEIRIARRKEIRKAQRLRYKVFYE
                     EGGAIAHGPGALVRRDICPFDKFCDHLLIVDLVARNAFGRVKPKIVGTYRLLRSEVAA
                     DHGGFYSEGEFDIAPLLARHAGKRFLELGRSCVHAKYRSKRVIELLWRGLWTYIQHYH
                     VDVMFGCASLP"
BASE COUNT          344 a          515 c          562 g          341 t
ORIGIN      
        1 gagcgcgcca agcgcgtcga actcggaatg ccggaggcga ttgcgatgcg ccaccgtctg
       61 ccgcatttca cccatgtctt cgccggcgac ggctattcgt cgggctatta ctcttaccta
      121 tggtcggaag tgctcgatgc ggacggcttt gccgccttcg cggaagcggg cgacattttc
      181 gatccagagg tcgcggcgcg cttgaaacaa tatgtctatt ccgccggcaa tcgtgccgat
      241 ccggacgcgg cttatctatc gttccgtggt cgtccagcgg cggcagaacc gttgctcaag
      301 aagcgcgggc tgatcgaggc ttaaaagcga taggctgccg caatccggtc gaaacattcc
      361 tgcgcgacgg cgcggcgagt caacggggct gtgagtacga ttggttcgca gaagatcagt
      421 tcgcaggtga gcggcggatt tttcatcagc gcgatgacgt gcggcagcag cgcgtcgtcg
      481 ccgatccagg gcgcataggg cgccgaatag cggatcgcga tgggctggat ggcgtaagtc
      541 tgcccgagct tttgtaagtc ctcgacgacg gcaaaatgcg ccgaatgaaa taggtgcggc
      601 ccgtccgcat gggttgtggc ctcgggaaac agcacgacat gcgcgccgtt ggcgatcgcc
      661 gccagcatag cggcattggt cgctgggata gtccgccgcg cggtgcgatc gacgaagatc
      721 gtgttcacga gttgcgcgaa tgtgcggatg atcggccagc gtccgacctc gcttttggcg
      781 acgaaggcgg ccgggaacag cgcgcccagt gccggaatat ccgtccagga aacgtggttg
      841 gtgaccacga gcgcagcaat cttaccgggc ttccaaccgc tcgccttcac ccggactttc
      901 acgagccaga ggacgatcgt gtagaaaagc cgtggcaaga cctgactgcg gcgcggcgcg
      961 gccgtgagca gcccccattg aataggtgcg aacagcaaaa atgagatgaa aattgctagg
     1021 aatagcgccg ccgccctcag gcaaacaccg gcccataaaa ggcttcgtga aatcaggtac
     1081 gaaatgggat aagaccttgc ctgatggccg cggaaccccg cgtcatcaaa gtttcgttcg
     1141 ggctggttag cctgatggga tcatatctga gcggatgatt cgataaaaca tgtggccgat
     1201 agagccgtcc ttcggcgccg tcatcccctt ctcaccgacg cggcgcctga tgcgcgccaa
     1261 gcgtgcggcg cccgtcgatg tgccgcgggt gttgggccgg ttcggcaatt tcgaaatccg
     1321 catcgcgcgg cgcaaggaaa tccgcaaggc gcagcggctg cgctacaagg tcttttatga
     1381 agaaggtggc gccatcgccc acggtccggg tgcactggtg cggcgcgata tctgcccctt
     1441 cgacaaattt tgcgatcatc tgctgatcgt tgatctcgtt gcccgcaatg cctttggccg
     1501 ggtcaagccg aagatcgtcg gcacctaccg gctgctgcgg agcgaggtcg cggcggatca
     1561 tggcggcttc tacagcgaag gcgagttcga tatcgcgccg ctgctggccc gccacgccgg
     1621 caagagattt ctggaactcg gccgctcctg cgtccacgcg aaatatcgct cgaaacgcgt
     1681 gatcgagctt ttgtggcgcg gcctctggac ctacatccaa cattaccacg tcgatgtaat
     1741 gttcggctgt gccagcctgc ca
//
LOCUS       DASWOG010000444         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_65353,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000444 DASWOG010000000
VERSION     DASWOG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65353"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1033
                     /locus_tag="VGB93_09220"
     CDS             248..1033
                     /locus_tag="VGB93_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146314.1"
                     /translation="MMTTLSEAAGAAALESALRACQQANERLAAILANTMSQTDPAMS
                     KGGVQTQFNWPKKIDTTGFDHSLSIARNLVVRRDTTRVYVAGCEGLRCLSKELGVPLF
                     KIGTTSADLLQRLAEISADRYAAAYSLNDETIVDQEFDHWKLMSMDFSLPRAVESPVW
                     IEPRALRVTLPKMLSGKQFEERLREALAPLSLANWLETEEAQRHLVTRGISKARGDRF
                     TPYGYGDSTRLSRADELYIFRHRIDMPRLCRLSEVIVQEAVQG"
     gene            1123..1872
                     /locus_tag="VGB93_09225"
     CDS             1123..1872
                     /locus_tag="VGB93_09225"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY0146315.1"
                     /translation="MPRTIMVFDLETRLDKSAVARAYQLDEGDHAWATSVIGDKFPPL
                     IFHEIICIGALRAVHSDGAWKVEALGAPHSKERDEKNLISDFAVAIEKHRPKLVTFNG
                     SSFDLPVLRYRGGLHQVFAPGLYQQPYFHRYGDASTALCDLLASYDGRGKVSLDVLCR
                     TWNIPGKPGTIDGSQVAGFAGDGRFTEIAQYCEHDVVATYKVWLRHELFAGRLTPEAF
                     ARSEDALADYIFCAGKFHLHAFLAPRGTSSN"
     gene            1996..>2239
                     /locus_tag="VGB93_09230"
     CDS             1996..>2239
                     /locus_tag="VGB93_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146316.1"
                     /translation="MVSPDEPVRALLGQDIAEADTKNQEAKSADKNVESTIDLFDVAE
                     IRRQIGDDQDDFGMEIGNHEPQALSNEEVERQRRLRA"
BASE COUNT          504 a          674 c          596 g          465 t
ORIGIN      
        1 caaagagtaa acgcgatcca catgtcagtt cctcagggga gcgctacccc ctatttttgg
       61 gttcacagag ttcgagcatg cacattgaga caaatacact ctggttctca cgactatcct
      121 tgcaccacca aggctttcaa ctgaaataaa gaggaatcag gctggcctcc agttccccct
      181 gaatgcgcct tcgtcgctgt agtgtgagat tcatatctca tacccccaat aaatccccag
      241 aaagagaatg atgacgacct taagtgaagc agccggcgcg gctgccttag agagtgcttt
      301 gcgcgcatgc cagcaagcaa acgagcgtct cgctgcgatt ttggccaaca ctatgagcca
      361 aacggacccc gcgatgtcaa aaggtggcgt tcaaacccaa ttcaattggc cgaagaaaat
      421 tgatacaaca ggcttcgatc atagcctgag catcgccaga aacctcgttg ttagacgcga
      481 cacgacccgc gtgtacgttg cgggttgcga gggattgagg tgtttatcaa aggagctggg
      541 cgtcccgctt ttcaagatcg gcacgaccag cgctgatctt ttgcagcgcc tcgccgaaat
      601 cagcgctgat cgctacgccg cggcctacag cctcaacgac gagaccatcg tagaccagga
      661 gttcgatcac tggaaactga tgagcatgga tttctctctg ccgagagccg tcgaaagccc
      721 ggtctggatc gaaccgcgcg cccttcgggt cactctgccc aaaatgctct cgggaaagca
      781 attcgaagag cggttgcgtg aagcgctcgc accgctctcc ttggcgaatt ggctcgaaac
      841 cgaagaggct caacgccatt tggtgacgcg cgggatcagc aaggcgcgcg gcgaccgctt
      901 cacgccgtac gggtatgggg atagcacgcg gctttctcgt gccgacgagc tctacatctt
      961 ccgtcacagg atcgacatgc ctcggctttg ccggctgtct gaagtgatcg tgcaggaagc
     1021 cgtccaaggc tgatccgctt attcgatcga cctcccttcg ttcccactcg tgcgctttcc
     1081 gtgcgcgaga accccctcgc ctaccctaaa taaggaataa ccatgcctcg cactatcatg
     1141 gtcttcgacc tcgagacccg cctcgataaa tctgcggttg ctcgcgccta ccaactcgac
     1201 gagggcgatc acgcttgggc gaccagcgtc attggcgata aatttccacc gttgatcttt
     1261 cacgagatca tttgcattgg cgctctacgg gcggtccatt ccgacggcgc ctggaaagtc
     1321 gaagcgcttg gcgcacctca ctcgaaagaa cgcgacgaga aaaatctcat cagcgatttc
     1381 gctgttgcga tcgagaagca ccgaccgaag ctcgtcacct tcaatgggtc gtcttttgat
     1441 ttgcccgttc tgcgctaccg cggcggcctg catcaagtct ttgcgccggg attgtaccag
     1501 caaccttact ttcatcgcta tggcgacgct tcgacggctc tctgcgatct cctcgcctct
     1561 tacgacgggc gcggcaaagt gagcctcgat gtcttgtgca gaacctggaa tattccgggc
     1621 aagcccggca caatcgatgg cagccaggtc gctggttttg ccggcgacgg ccgcttcacc
     1681 gaaatcgcgc aatattgcga gcacgacgtc gtggcgacct acaaggtctg gctccggcac
     1741 gagctctttg ctggccggct cacacccgaa gcttttgcgc ggagtgaaga tgccctcgcc
     1801 gattacatct tctgcgccgg caagtttcat ctgcatgcgt tcttggcgcc acgcggcacg
     1861 tcgtcgaact agcccgcgcc gctataactt agcttttcca cgccgcgaca agtaccgcgt
     1921 aaagcagcag cctttctgaa gctgccgact ttctcccgac ctgaaacgat tctcaaactc
     1981 aaaggaacga tgacgatggt gtcaccggac gaacccgtgc gcgcactgct tggccaagac
     2041 atcgctgaag ccgatacgaa gaaccaagaa gcgaaatccg cggacaaaaa tgtcgaatcg
     2101 acgatcgatc tttttgacgt ggcggagatc cgccgacaga tcggcgacga tcaagacgat
     2161 ttcggcatgg agatcgggaa ccatgagccg caggccctct caaacgaaga agtcgaacga
     2221 cagcggcggc tcagggccc
//
LOCUS       DASWOG010000445         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_358380,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000445 DASWOG010000000
VERSION     DASWOG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358380"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1475)
                     /locus_tag="VGB93_09235"
     CDS             complement(87..1475)
                     /locus_tag="VGB93_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146317.1"
                     /translation="MGESFDIAIIGGGYTGAVLALHLLRRARAGARIAVIEPRADVGS
                     GVAYATPDEQHRINVPADRMGVSAAPQDRFDIWLKTQHPELIGDGDPERTYVPRRWFG
                     IFVRDRLAEAIRTSAAVFRHIQAEATSVRAAEGGAEIALSDGRKLRARHVVVAVSHGL
                     PALPRGIPATLVGTPGFLENPWNVERLREIPRDGAVLIIGTGLTMADVIASLLARGHC
                     GPITAISRHGLLPRKSGPASVPPGLDLSAWPSASVAHYVKSIRAEMDRVEAEGLSWRG
                     VFTALRVQSGYLWSRLTHADKHRFLRHLKTFYDVHRYRMAPETYELIEAAESRGQVRI
                     LAAHVIGVERTAQGFDVAFRRRGAAADETLRVAGIVNCTGPKQKLRADPTNFLGGLIA
                     AGIAKADPLGLGLAVDGDFRVVGNATNFYALGPLTRERFGDIYGAPEIQLHAERLAGQ
                     LAAALAKENAAV"
     gene            complement(1856..2596)
                     /locus_tag="VGB93_09240"
     CDS             complement(1856..2596)
                     /locus_tag="VGB93_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0146318.1"
                     /translation="MRKAISAAALLLTVGTTSVFAADLPSAKGAPVYTPAPPVLSWTG
                     VYVGGQIGIQWGQTSWDRFDPTNTALIASELPYSNKGVVGGGHIGYNYQVSQFVFGLE
                     GDVEDTNFNGNGTSNGNTWANTTRADIEASIRARAGIAWNQILFFLTGGGTYAGFHNT
                     AQNPPGNFYAGDSFGRIGWTGGGGVEYAIDPNWSILIEDRFSEYGHHDLDTGTEFIHE
                     GLWDNRVEAGFSYRFGDVLPPAPVVAKY"
     gene            complement(2712..4835)
                     /locus_tag="VGB93_09245"
                     /pseudo
     CDS             complement(2712..4835)
                     /locus_tag="VGB93_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT         1046 a         1382 c         1394 g         1018 t
ORIGIN      
        1 gtaatgattt ggctcatgag caaggtccgc gctgcgaatc tcccgatgat ggctggatcg
       61 cgggcggtgc gggagcaata aattgcctaa accgcggcgt tttccttggc gagcgcggcc
      121 gcaagctggc ctgcgagccg ctcggcatga agctggattt ccggcgcgcc ataaatgtcg
      181 ccaaaccgtt cgcgggtgag cggcccgagc gcgtaaaagt ttgtcgcgtt cccgacgact
      241 cgaaaatctc catcgacggc aagtccgagg ccgagaggat cggccttcgc aatacctgcg
      301 gcgatgaggc cgccgagaaa attcgtcgga tcggccctga gcttctgttt cggccccgtg
      361 caattgacga tgccggcgac acggagcgtt tcgtcggcgg cagcgccgcg gcgccggaag
      421 gcgacatcga agccctgcgc cgtgcgttcg acacctatga catgcgccgc cagaatcctg
      481 acctgcccac gcgattcggc ggcttcgatc aattcatagg tttccggcgc catgcggtag
      541 cgatggacgt cgtaaaacgt tttgaggtga cgcaggaacc ggtgcttatc agcatgtgtg
      601 agtcgcgacc agagatatcc gctctggaca cgcaaggcgg tgaagacacc gcgccaggaa
      661 agtccttccg cctcgacgcg atccatctcg gcgcgaatcg atttcacgta atgcgcgact
      721 gatgccgacg gccatgcgct gagatcaaga cctggcggca cgctcgccgg cccactttta
      781 cgcggcagaa gaccatgccg cgaaattgca gtgattggac cgcaatggcc tcgcgccaga
      841 agcgaggcga tgacatcggc catcgtcaac ccggtgccaa tgatgagcac ggcgccgtcg
      901 cgcggaattt cccggagacg ttcgacgttc cagggatttt cgagaaagcc cggcgttcca
      961 acaagcgtcg cgggaatgcc gcgtggcaac gccggcaatc catgactgac cgcgaccacc
     1021 acatggcgcg cacgcaactt cctcccgtcg ctgagggcaa tttcggcgcc gccttccgca
     1081 gcccgcacgg atgtggcctc ggcctgaata tgacgaaaaa cggccgccga agtccgtatc
     1141 gcctcggcaa gccggtctcg cacgaaaata ccaaaccagc ggcgcggcac gtaagtgcgt
     1201 tcagggtcgc cgtcgccgat cagttccgga tgctgcgtct tgagccaaat gtcgaagcga
     1261 tcttgaggcg ccgcgctcac gcccatccgg tcggccggca cattgatgcg gtgctgctcg
     1321 tccggcgtcg cataggcgac accggagccc acatctgcgc gcggctcgat aacagcgatt
     1381 ctcgccccgg cacgcgcgcg gcgcaggaga tgcagcgcaa gaacagcgcc cgtgtagccg
     1441 ccgccgatga tcgcgatgtc gaaactttca cccaagagag cgccccatca taaatcaaga
     1501 ttcgctcgtg tacatctcac tcgcgtattg ggacattttc tagttagatt ggcttaaaag
     1561 gaaatactgg caatcatttt ttcttgattt ccgtcatctg cgtttttcac atctccctat
     1621 cgttcttgca tcgaaaattt tcttctccga gcgacgatgt aggggcgaag ataaaaccgt
     1681 ggacaccaag gaattgcata cgaaggccgg gatcaagctc gatcgcgtca tcgaatagag
     1741 cgctttccag cgcgcatcgc caaacaaaag agccgctgca aaatgcagcg gctcttttgt
     1801 tttttaaatg aagatgaccg cgttccccgc cagccgtgaa gccgggttga ccggtttagt
     1861 atttcgcaac gaccggcgca ggcggcagca catcgccgaa cctatagcta aagcccgcct
     1921 ccacccgatt atcccacaga ccctcgtgga taaattccgt gccggtatca agatcgtgat
     1981 gaccatattc cgaaaaccga tcctcgatga ggatcgacca attgggatca attgcgtatt
     2041 cgacgccacc accgccggtc caaccgatgc ggccaaagct gtcacccgca taaaaattgc
     2101 cgggaggatt ttgcgccgta ttatggaagc ccgcgtaagt accgcccccc gtgagaaaaa
     2161 atagtatttg attccaggct ataccggctc gggctcgaat agaggcttca atatcggcgc
     2221 gcgttgtatt cgcccatgta ttgccatttg aagtgccatt accgttgaag ttcgtgtcct
     2281 cgacatcgcc ctcgaggccg aaaacgaatt ggctcacctg ataattgtaa ccaatatgac
     2341 cgccgccgac gacaccctta ttgctataag gcaattctga ggcgatcaaa gccgtgttgg
     2401 ttggatcgaa gcgatcccag ctggtctgtc cccactgaat accaatctgg ccaccgacat
     2461 aaacaccggt ccagctgagg accggaggag ccggcgtata aaccggcgca ccttttgcgg
     2521 aaggcaagtc ggccgcaaag actgaagttg tccccaccgt caaaagcagc gctgctgctg
     2581 aaatggcttt gcgcatttct tccccccgaa atgaatatgc atcaactgtt tattcgcgct
     2641 tccgtcagtc gtaacttttt aaagaaggac cggcgaactg cccgccggtc ctgaagccgg
     2701 aatttaacgg ctcaatattt ggccacgagc ggcggagccg gtggcggcgc gtcattcagc
     2761 gagtatttga gaccgacgaa ggcttcgaat ggcggcgcaa tcgtgatggc gcgtgggtct
     2821 tcgctcgcaa aaaggccggc cggaccgcct gtaagctgat tggtcgtcga atctgtctcg
     2881 tacaaggtgc caaaggtcgc gccacgaaaa ttggcggcgt tgttaatcag accgtagatc
     2941 tgaaggtcct tggtcacctg atacgacgtg cgcagatcga tcgtggcata gccagagacc
     3001 cgcgggttga gacctggccc gagcgtgttg ttgtccgccc cgaaataata ttggctggag
     3061 cgatagacca cgtcaccgcc cactttccat cccggcagga cttcataatc gatgccgaat
     3121 ttgaacttat ggttcggaat gcctggaata ttatcgccag gtgaaacctg tatctcgccc
     3181 gtatccggat cggaggttgg attgttcggc gacgaaagcg ctattgacgt caggtatatc
     3241 gcccggatgt aggaatagtt gacgtaaaca tcccaacggt cggtcgtata ggtggcgccg
     3301 atgtcgacac cttcgcgcag cgtattacca gcgttggtga aaaacccgag agtcggatca
     3361 attgggcttg gaacattcgt aatatcgtcc tggttctcgg tgtgatagcc ggcaatgctc
     3421 cagtcaagcc ggcccggtgc ccagccccaa ttgattacgt tgctgccctt gaagccggct
     3481 tcgacggtcc gggcggcgat ctacttcagc ggcgggtcgg cggagaggaa attatcgatg
     3541 acgcaaggcg tctgcgggct cgagcaaccg atttcaagag gcgtcggcgc gcgattggct
     3601 tctgaatagc tcgcgtaggc tgcgatatcc ggcgtgatct tgaatgtcag gccgacaaca
     3661 ggattgatac ggtggaaact ttgggtccca ttgatattgg gctcgactgt tccgtcggtg
     3721 gcgataccac cagactcatt gccgcccata tcggccagcg aaataatggc gtcgttgaag
     3781 cgtgcgccgg cgtggatggc taatctgtcg gtgatgtcga agttatcaag cacgtagacg
     3841 ccgatatagg tattctgcgc cttcacggac accgggacaa tagagttatc gggatcgtcg
     3901 ataaggttat tggtaaacgg cacgacgaaa ttcggcggca gcgtgcctag agtgctgctg
     3961 ccgttaaaat tggtccagcc atgatcgaca ctgacgccgg cggtgatcgt gttgttgtga
     4021 ccaaggatac gatcggtatc ggtcaattgc cccgtcgcgc ccgtgctcag cgtgtgcgtc
     4081 caattgctat cgatctctcc caaaggctgg ccgtcgggca agttaaacgg attgctgacg
     4141 ttcgttgccg gtatgccggc acccgagact ggatcggtgc cattacaggc cgcattcgtg
     4201 ccgcccgggc ccagaccggc gccggcgtcg caattaaaat aatcgctgat gttgccatca
     4261 tgatgcactg acgcatattg gcggaaatag gtattgccat tgaacgtcag cgtcggcgta
     4321 atgtgtgttt catccgacaa cgtgatcatt tccgcggcgc tgtccgtcgt ctgcggcgtc
     4381 gtgaaaacgg cccaggggtt ttgctgggcg agttgcagcg gggtggtgcc agcgatgcca
     4441 acgcccgcag cgccacctgt catgctcaaa tggagttcgc tgttccaggc gcgatagcca
     4501 atgtcgccat aagcgcgctc cgcgtccgac tggccgaaat agcggaagcc gttggtgcca
     4561 atgccatcaa tgtcgaagta ataggcccaa tcgcccttgt tgacaccgta ttgggcgtaa
     4621 ccctgaccgg tataatccat accgccctgg agattgattt ccgtgccttg ccaattgaag
     4681 ccgttcttca tttgcatgac caccgcgcca gcgagcgcgt tgaggccgaa gaccggattg
     4741 ccggttacga actgtgccgt gctgatcgcc gacggcggga tcaaatccca attcacgacg
     4801 tcgccgtagg attcgttgat tcggacaccg ttcatataaa
//
LOCUS       DASWOG010000446         4479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_322599,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000446 DASWOG010000000
VERSION     DASWOG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322599"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGB93_09250"
     CDS             complement(<1..208)
                     /locus_tag="VGB93_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEY0146319.1"
                     /translation="MTDHTVRAYDDELEALGRKIAEMGGIAEQMLSNAMDALATLDSD
                     LARVTISMDLRLDVLQRDVEEQAIL"
     gene            complement(236..1045)
                     /gene="pstB"
                     /locus_tag="VGB93_09255"
     CDS             complement(236..1045)
                     /gene="pstB"
                     /locus_tag="VGB93_09255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0146320.1"
                     /translation="MTTAGPSVVAATTPVKKALPTKIDVKNLQFYYGAHHAVKNVNLP
                     LYSNKVTALIGPSGCGKSTLLRVLNRMYDLYPGQRAEGEVLLDGTNILSPSQDLNLLR
                     SRVGMVFQKPTPFPMSIFDNIAFGVKLYERVSRADLEGRVETALRGAALWEEVKDKLN
                     ASGLSLSGGQQQRLCIARTVAIHPEVILFDEPCSALDPISTAKVEELIDELKDKYTIA
                     IVTHNMQQAVRVSEHTAFMYLGELVEFGLTHQIFTAPENQRTQNYVTGRFG"
     gene            complement(1042..1881)
                     /gene="pstA"
                     /locus_tag="VGB93_09260"
     CDS             complement(1042..1881)
                     /gene="pstA"
                     /locus_tag="VGB93_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0146321.1"
                     /translation="MALYENRKRANAIWLGLCYASVVFGLTFLVLILITLFWNGFSGL
                     SLKVFTQSTPAPDSEGGLLNAIVGSLIITILGVLIGTPLGILAGTYMAEYGRNSKLAS
                     VVRFINDILLSAPSIVVGLFVYQVMVTRMGHFSGIAGAVALAVIVIPIVVRTTENMLL
                     LVPGSLREAASALGLPRSTVVQRIAYKAAQAGLITGVLLAIARVSGETAPLLFTALNN
                     QFFSTDLNAPMASLPSIIFQFAMSPYKGWQSLAWTGALLITLTVLGLSILARTLSSQG
                     RTR"
     gene            complement(1884..2876)
                     /gene="pstC"
                     /locus_tag="VGB93_09265"
     CDS             complement(1884..2876)
                     /gene="pstC"
                     /locus_tag="VGB93_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0146322.1"
                     /translation="MADIAVKSGRVIDEAAARARVLTGFKFRDTAFRWLTLSAAVIVL
                     LLLGGVIATLINGSIPAFKAFGFRFLFTNVWNPATGQYGAPAAVYGTVITSIIAMVIA
                     VPIGLGVAIFLTELCPHALRRPIGTAIELLAGIPSIIYGLWGLFVLAPFLQQHVQPLL
                     IGLFAKVPVFNILFAGPPYGIGLLTAGLILAIMVIPFVSAVSRDVFDTVPPLLKEAGY
                     GNGFTTWEVISNIVIPYTRVGVLGGVMLGLGRALGETMAVTFVIGNADVIPTSLMAPG
                     TTISAAIANEFTEAVDELYTSSLIELGLILFVITFVVLSISRLMLMRLERQAGR"
     gene            complement(3070..4116)
                     /gene="pstS"
                     /locus_tag="VGB93_09270"
     CDS             complement(3070..4116)
                     /gene="pstS"
                     /locus_tag="VGB93_09270"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0146323.1"
                     /translation="MSFKTLAKVGLIAGAIIGAVVAAEATDISGAGATFPYPIYSKWA
                     AAYKTETGDSLNYQSIGSGGGIKQIEARLVTFGATDKPLEVADLDKNGLVQWPQVMGG
                     IVPVVNLDGVTAGSIVLDGPTLAQMFLGEIKTWDDPAIAKLNSGVKLPSTAIAVVHRA
                     DGSGTTFNFTDYLSKVSPDWKAKVGSATAVQWPVGIGAKGNEGVANNVVQTKGAVGYV
                     EYAYALQNKMTYTKLINGAGKVVAPTAGAFAAAAANADWAHAPGFHLILTNQPGDASW
                     PITAATFILLPKQPKDEPAAKAALKFFAWAFDKGGPAAESLDYIPIPAAVVTLIKKTW
                     ADDVKGADGKALVN"
     gene            complement(4254..>4479)
                     /locus_tag="VGB93_09275"
     CDS             complement(4254..>4479)
                     /locus_tag="VGB93_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146324.1"
                     /translation="AVRDFGTGIAPEHLPRLTERFYRVDAGQSRAKGGTGLGLAIVKH
                     IIARHGGRLNIESKWREGATFIVTLPLFES"
BASE COUNT          931 a         1303 c         1418 g          827 t
ORIGIN      
        1 tcaggatcgc ctgctcttcc acgtcgcgtt gcagcacgtc gagccgcaaa tccattgaga
       61 tcgtcacgcg cgcaagatcg gagtcgagcg tcgcgagtgc atccatcgcg ttgctcagca
      121 tttgctcggc gatgccgccc atttccgcga tcttgcggcc aagggcttcg agctcgtcgt
      181 cgtaggctct gacagtatga tcggtcatgg caatagggtc ctgaaaccgg aaacctcagc
      241 cgaagcggcc ggtgacgtaa ttctgcgtcc gttggttttc gggcgcggtg aagatttgat
      301 gcgtcagtcc aaactcgacg agctcgccga gatacatgaa ggccgtgtgt tcggagacgc
      361 gaaccgcctg ctgcatgtta tgcgtgacga tggcgatcgt gtacttatct ttcagttcgt
      421 caatcaactc ttccactttc gcggtggaaa tcggatcgag cgccgagcag ggctcgtcga
      481 acaggataac ttccgggtgg atggcgacgg tgcgggcgat gcacaggcgc tgctgctgac
      541 cgccggagag cgacaggccg ctggcgttga gcttgtcctt gacctcttcc caaagcgcgg
      601 cgccgcgcaa cgccgtttcc acccgtcctt cgagatcggc gcgtgacacg cgctcgtaaa
      661 gcttaacgcc gaaagcgata ttgtcgaaga tcgacatcgg gaagggcgtc ggcttctgga
      721 ataccatgcc gacacgcgag cgcagcaagt tcagatcctg cgatggcgag agaatattcg
      781 tgccgtcgag taaaacctca ccctcggccc gctggccagg ataaagatcg tacatgcggt
      841 tcaacacgcg cagcaaggtt gacttgccgc agccggacgg cccgatgagc gccgtgacct
      901 tattgctgta gagcgggaga ttgacgttct tcaccgcatg gtgcgcgcca taatagaatt
      961 gcagattctt cacgtcgatc ttcgtcggaa gagccttttt caccggcgtg gtcgctgcca
     1021 ctacggaagg gcctgcggtc gtcatcgtgt acgtccttgc gaactgagag tccgcgccag
     1081 gatcgacaat ccgagcacgg taagcgtgat gaggagcgcg cctgtccagg cgaggctctg
     1141 ccagcctttg taggggctca tcgcgaattg gaagatgatc gatggcaggc tcgccatcgg
     1201 cgcattgagg tcggtcgaga agaattggtt attcagcgcc gtaaaaagca atggtgccgt
     1261 ctcgccgctg acgcgggcga tcgcgaggag gacgccggtg atcagtcccg cctgcgcagc
     1321 cttgtaagcg atccgttgca cgacggttga gcgcggcaga cccagtgcgc tcgccgcctc
     1381 gcgcagagag cccgggacga gaagcagcat gttctcggtg gtgcgcacga cgatcgggat
     1441 cacgatgaca gcgagcgcca ccgcgccggc aatgccggag aaatgcccca tgcgcgtcac
     1501 catcacctgg tagacgaaca ggccgacaac gatcgacggt gcgctgagca ggatgtcgtt
     1561 gatgaaacgc acgaccgagg cgagcttcga attgcgcccg tattcggcca tgtaggtgcc
     1621 ggcgaggatg ccgagcggcg taccgatcag gacaccaagg atggtgatga tcaggctgcc
     1681 gacgatcgca ttgagaagac cgccttcgct atctggcgcc ggggtgcttt gcgtaaatac
     1741 tttaagcgaa aggccggaga agccgttcca gaaaagcgta ataagaatga gcaccagaaa
     1801 cgtcaggccg aaaacgaccg aggcgtagca gaggccgagc cagatggcgt tggcgcgctt
     1861 gcggttttcg taaagtgcca tgattaccgc cccgcctgcc tctcgagccg catcagcatc
     1921 agccgcgaga tcgagaggac cacgaatgtg atgacgaaaa ggatgagccc caactcgatc
     1981 agcgacgacg tataaagttc gtcaacggct tcggtgaact cattggcgat cgcggcggaa
     2041 atcgttgtgc cgggcgccat cagcgacgtt gggatcacgt cggcattgcc gatgacgaat
     2101 gtgaccgcca tggtttcgcc gagtgcccga ccaaggccaa gcatgacgcc gcccagcacg
     2161 ccgacccgcg tataggggat gacgatgttg ctgatcacct cccaagtggt gaatccattg
     2221 ccatagccgg cttccttaag gagcggcggg accgtgtcga acacgtcgcg cgacacggcg
     2281 gagacgaagg gaatgaccat gatcgcgaga atgaggccgg ccgtcagcag gccgatgccg
     2341 tagggcggcc cggcgaacag gatattgaac accggcactt tggcgaacaa accgatcagc
     2401 aagggctgaa catgttgctg caggaaaggc gcgagaacaa acaggcccca gagcccgtag
     2461 atgatcgaag gaatgccggc gagaagctca atcgccgtac caatcggacg gcgcaaggca
     2521 tgcgggcaaa gttcggtaag gaagatcgca acgccgaggc cgatcggcac ggcaatgacc
     2581 atcgcgatga tggatgtgat caccgtgccg tagaccgccg ccggggcacc atactggccg
     2641 gtggcagggt tccagacatt ggtgaagaga aagcggaagc cgaacgcttt aaaggcggga
     2701 atcgagccgt tgatcagcgt cgcgatgacg ccgccgagga gcagcaggac gatgacagcg
     2761 gcgctgagcg tcagccaccg aaacgcggtg tcgcgaaact tgaacccggt caatacgcgg
     2821 gcgcgggcgg cagcttcgtc aatcacgcgg ccactcttca ctgctatatc cgccaaacgt
     2881 cgcctccgca cttagcgcaa gaaattcgtt cgcgatgcgc ttcgcttatt ttcaagccgc
     2941 acaatctacg ccggatgtcg gcgcctgcgt catgcgtgag acttgccgcg gcgatctgtg
     3001 cccgcggcgg tcctttactg tgagacgcgg cggtgacgcc gcgcctcaaa ctttcctaag
     3061 cgaagccgct tagttcacca gagccttgcc gtcagctccc ttgacgtcat ccgcccatgt
     3121 cttcttgatg agcgtcacaa ccgccgccgg gatcgggatg tagtccagag actcggccgc
     3181 cggaccgccc ttgtcgaagg cccaagcgaa gaatttcagc gccgctttgg cggccggctc
     3241 gtccttcggc tgcttcggca acaggatgaa ggtcgctgcc gtgatcggcc aggacgcatc
     3301 gcccggctgg ttggtgagga tcaggtggaa gcccggggca tgcgcccagt cggcattcgc
     3361 cgccgcggct gcgaaagcac ccgccgtcgg ggcgacgacc ttgccagcgc cgttgatgag
     3421 cttggtgtag gtcatcttgt tctggagagc gtaggcatat tcgacatagc cgacagcgcc
     3481 cttggtctgg acgacattgt tggcgacgcc ttcgttaccc ttggcgccaa tgccaaccgg
     3541 ccattgcacg gcggtcgcgg aaccgacctt cgccttccaa tccggcgaaa ccttcgacag
     3601 gtagtcggtg aagttgaagg tggtgcccga accgtcggcg cggtggacaa cggcaatcgc
     3661 ggtcgagggc agcttgacgc ccgagttcaa cttggcgatt gccgggtcgt cccaggtctt
     3721 gatctcgccc aggaacatct gggcgagggt cgggccgtcg aggacaatcg aaccggcggt
     3781 gacgccgtcg agattgacga ccggcacgat gccgcccatg acctgcggcc attgaacgag
     3841 accgtttttg tcgagatcgg cgacttcgag cggcttgtcg gtcgcgccga aggtcacgag
     3901 gcgggcttca atctgcttga taccgccgcc ggagccgatc gactgatagt ttaggctgtc
     3961 gccggtttcg gttttatagg ccgcggccca tttggaatag atcggatagg ggaacgtcgc
     4021 gccggcaccc gagatgtcgg tagcttcggc ggcgaccacg gcgccaataa tggcaccagc
     4081 aatcaaaccc accttggcga gggtcttgaa actcatctta agcctctcaa aactgagaaa
     4141 atagcgctgt atgtctggcc ttcggcccaa ccgcgcggcc gcaccctagg aaggtcgggc
     4201 aacgcattta cgacggtcat atgacgttta catgacgatg ggggaagggt cgtctagctc
     4261 tcgaaaagcg ggagcgtcac aatgaaggtc gcgccttctc tccatttgga ttcaatattg
     4321 aggcggccgc cgtggcgcgc gataatatgt ttaacgattg caaggccaag ccccgtgccg
     4381 cccttcgcgc ggctctgccc ggcatcgacg cgataaaagc gctccgtcag gcgtggtaaa
     4441 tgttccggcg cgatgcctgt gccgaaatca cgcacggcg
//
LOCUS       DASWOG010000447         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_46788,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000447 DASWOG010000000
VERSION     DASWOG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46788"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VGB93_09280"
     CDS             <1..1253
                     /locus_tag="VGB93_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146325.1"
                     /translation="FADDFADAEGFGWTVGETSFDWGKLIAAKDKEISRLSAIYRANL
                     EKTGVAFVQSRAIVEDAHTVRILAENRTVTARYILIATGGAPFLGPEIPGRELAITSN
                     EIFDLKEQPKRILIVGGGYIAVEFASLLERLGSKVIVALRGENILRGFDEDARCFLCD
                     NLSERGIEFKFNDMPTRLEKKDEVIHATLALGETLEVDQVMLAIGRRPATQGIGLEKA
                     GVRLDAVGAVCVDSFSQSSVTSIYAVGDVTNRVDLTPVAIREGHAFADTVFGGKPRVV
                     DHMRLPTAVFTTPELGTVGMTEEEARLKHSVVDVFKTSFRPLKATLCESCDRVFMKII
                     VDGETDRVLGVHIIAGDAGEMIQLLGIIIKMGATKADFDAAMAVHPTSAEELVTLHTR
                     TMRYEQPKLDPGEAPALMDEEQMP"
     gene            1386..2771
                     /locus_tag="VGB93_09285"
                     /pseudo
     CDS             1386..2771
                     /locus_tag="VGB93_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175240.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
     gene            2860..3114
                     /locus_tag="VGB93_09290"
     CDS             2860..3114
                     /locus_tag="VGB93_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146326.1"
                     /translation="MAWKTTIAVLAALAFSAPAFATEMMQGGHHACMSGGKKGGCMEH
                     HYTGHSACMHGGKKGGCMMHHSMGHHATPSGDKKAGAMTH"
     gene            complement(3161..>4288)
                     /locus_tag="VGB93_09295"
     CDS             complement(3161..>4288)
                     /locus_tag="VGB93_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ceramide glucosyltransferase"
                     /protein_id="HEY0146327.1"
                     /translation="LAWALALTCLVLVVLNLISDGIAIWHSKPCTTPLPPAPDAPPVS
                     IVRPCCGIDNFCEDTLGSSFKLDYPRYEVIFGVARSNDPVIPVLQKLIAANPRVPAQL
                     IIGDERVSANPKLNNCVRGWEAARHDWIILADSNVLMPRDYIQRLMAAWKPTTGLVCS
                     MPQGSHPRNVWAELEIAFLNTYQARWQYAADGVGTGFAQGKNMLWRRDILDAAGGIRA
                     LAMEIAEDAASTKVVRSAGLHINLVDSPFPQPLGKRTLRDIWSRQIRWARMRRKTFPL
                     HFLPEILSGSATPAVVGAYAGALFGFNPLLAAGLVLAVLYSGETALALSNKWHFSWRM
                     PFLFLLRDLMLPIVYVDAWVVNDFVWRGNEMTMREKERPST"
BASE COUNT          863 a         1262 c         1350 g          813 t
ORIGIN      
        1 gttttgcgga tgactttgcc gatgcggaag gttttggctg gacggtcggg gaaacctcct
       61 tcgattgggg caaacttatt gcagcgaagg acaaggaaat ttcccggctt tcggcgatct
      121 accgcgcaaa cctggaaaag accggcgttg cgttcgtcca aagccgcgca atcgtcgaag
      181 atgcgcatac ggtccgcatc ctggccgaaa accgcaccgt cacggcgcgt tacatcctga
      241 tcgcgaccgg cggcgcgccc tttctcggcc cggaaattcc cggccgggaa ttggcgatta
      301 cgtcgaacga gattttcgat ctcaaggaac agccgaagcg catcctcatc gtcggcggcg
      361 gctacatcgc ggtcgaattt gcctcactac tcgagcgcct cggcagcaaa gtcatcgtcg
      421 ctttgcgcgg cgaaaatatc ctgcgcggct ttgacgaaga cgctcgttgc ttcttgtgcg
      481 ataatttaag tgagcgagga attgaattta aattcaatga tatgccgaca agattggaga
      541 aaaaagatga agtgattcac gcgacgcttg cgctaggtga aacccttgaa gtcgatcagg
      601 tgatgctggc gatcggccgg cgtccggcga cgcaaggtat cggcctcgaa aaggctggcg
      661 tccggctcga tgcggtcggt gcggtctgcg tcgattcctt ctcgcaatcc tcagtgacct
      721 ccatctatgc ggtcggtgat gtgaccaatc gtgtcgatct gacccctgtc gcgatccgcg
      781 aagggcacgc ctttgccgat acggtcttcg gtggcaagcc gcgtgtggtc gatcatatgc
      841 gtctgccgac agccgtcttc acgacaccgg aactcggcac cgtgggcatg accgaagaag
      901 aggctcggct gaaacatagc gtcgtggacg ttttcaagac gagtttccgg ccgctcaagg
      961 cgacgctttg cgagagctgc gaccgcgttt tcatgaagat catcgtcgac ggcgagaccg
     1021 accgtgtgct gggcgtccat attatagcgg gcgatgccgg ggaaatgatc caacttctcg
     1081 gaatcattat caaaatgggc gcaaccaaag ccgatttcga tgccgcgatg gcggtgcacc
     1141 cgacctcggc ggaggaattg gtgacgctgc atacccgcac catgcgctat gagcagccga
     1201 agctggatcc gggcgaggcg ccggcgttga tggacgagga gcagatgccc tgatcggggc
     1261 tgcctggttc cgcggcgcgg cctctgccgg gacgtcctaa ttgcgggtcg caatcggctt
     1321 cacatatatg ttaaagaccc ggcgcgttca ttgccccggc aagtgctgcc aactggatga
     1381 gactcatgcc ttccgaaccc tggtcgccaa aaacttggcg gacaaaatcc atcgaacagg
     1441 cgccggttta cccggacatc gccgcgctct cggatgtgga gcggcagctg gcgtcgtttc
     1501 cgccgctcgt ctttgccggc gaggcgcgca agctgaaggc gctgctggcg aaggcctcga
     1561 agggagaggc gttcctgttt cagggcggcg attgcgccga gagcttcgcc gaacatgcgg
     1621 ccgacaatat ccgcgatttc ttccgcgtct tcctgcagat ggcggtagtg atgaccttcg
     1681 gcgccgcgct gccggtggtc aaggttggcc gcgtcgccgg ccaattcgcc aagccgcgtt
     1741 cggagccttt cgagaagaag ggcgatgtgt cgctgccgag ctatcgcggc gacattgtca
     1801 atgacattgc cttcacccag gcggcgcgga tccctgatcc gcagcgccag ctcgatgcct
     1861 atcggcagtc agcggcgacg ctcaatctgt tgcgcgcctt cgcctcgggc ggttatgcca
     1921 atctcgagaa tgcgcatcgc tggatgctgg gcttcatcaa ggacagcccg caatcggccc
     1981 gctaccagga gcttgcggat cggatcaccg agacactcgg cttcatgcgg gcgatcgggc
     2041 ttgaccccga gtcgcatccg gaattgcggc agacggattt ctatacgtcg catgaggcgc
     2101 tgctgctcgg cttcgaggag gcgctgactc gcgtcgattc gacgacaggc gattattacg
     2161 cgacctcagg ccatatgctc tgggtcggcg accgcacgcg ccagctcgat catgcgcata
     2221 tcgagtatct gcgtgggatc gaaaacccgc ttggcctcaa atgtggcccg tcgctgacgc
     2281 cggaaaacct gatccagctt atcgaccggc tcgatcccgc caatgagccg ggacggctga
     2341 cgctgatctg acgcttcggc gccgataaga tcggcgacca tctgccgaag ctgatccgcg
     2401 ccgtgcagcg cgagggccgc aacgtgctgt gggcgtgcga cccgatgcac ggcaacacga
     2461 tcaaggccgt gtcgggctat aagacgcggc ctttcgagcg catcatgagc gaaatccgca
     2521 gcttcttcgc ggtgcatcat gccgaggggt cttatgccgg cggcgtgcat ctcgaaatga
     2581 ccggcaagaa cgtcaccgaa tgtaccggcg gcgcgcgggc aatctcagag gccgatctgt
     2641 cggaccgcta ccacacctat tgcgatccgc gtctcaacgc cgagcaggca atcgaagtcg
     2701 ccttcctcgt cgcggaactg ctcaaggctg agcgactgca acgcccactg gcggaagtcg
     2761 cggcggaata ggccgtcggc cgtcgttcac gacgtgttcc tcctgtgtta tacgaacaag
     2821 gttcgtcagt cgaagaactg cgacacagga ggaaagtcca tggcgtggaa aacgacgatt
     2881 gcggtgctgg ccgcgttagc cttttcggcc ccagcctttg ccactgaaat gatgcaaggc
     2941 ggccatcacg cctgcatgtc cggcggcaaa aagggcggct gtatggagca tcattacacc
     3001 ggccacagcg cctgtatgca cggcggcaag aagggtggct gcatgatgca ccactcaatg
     3061 ggccatcatg cgacgccgtc cggcgacaag aaagcgggtg cgatgacgca ttagcggcat
     3121 cgcaactacc attgtggaat ttttagccgg ccgagtgcga ttacgtgctc ggccgttcct
     3181 tttcgcgcat cgtcatttcg ttgccgcgcc agacgaaatc attgaccacc catgcgtcga
     3241 cgtagacgat gggcagcatc aggtcgcgta acaggaagag aaacggcatc cgccaggaga
     3301 agtgccattt gttggacagg gccaaagcag tctcgccgct ataaagcaca gcgagaacga
     3361 ggccagcagc cagcagcggg ttgaacccaa acagagcgcc cgcataggca ccgacgacgg
     3421 cgggtgtcgc agaccccgac aggatttcgg gcaggaaatg cagcggaaac gtcttgcgcc
     3481 gcatccgcgc ccagcggatt tgccggctcc agatgtcccg caatgtccgt ttgcccaagg
     3541 gctgcgggaa gggactatcg acgagattga tatggaggcc agcggaccgc acgaccttcg
     3601 tggaggcggc gtcttcggcg atctccatgg cgagtgcgcg gatgccgcct gcggcgtcga
     3661 gaatatcgcg gcgccagagc atgttcttac cctgggcaaa gccggtgccg acgccatcgg
     3721 cggcatattg ccaacgcgcc tgataggtat tgaggaaggc gatctcgagt tcggcccaga
     3781 cgttccgcgg atgcgatcct tgcggcatcg agcagacaag gccggtcgtc ggcttccagg
     3841 ccgccataag ccgctggatg taatcgcgcg gcatcagtac attggaatca gccagaataa
     3901 tccagtcgtg acgcgccgct tcccagccgc gcacacaatt gttgagcttc ggattggcgc
     3961 tcacccgttc gtcgccgatg atcagctgcg ccggaacccg cggattggcc gcgatcagct
     4021 tttgcaggac cgggatgacc gggtcattgc ttcgggcgac gccgaagatg acctcgtagc
     4081 gcggatagtc gagcttgaaa ctcgacccga gcgtgtcctc gcagaagttg tcgatgccgc
     4141 aacaggggcg cacgattgag acggggggcg cgtccggggc tggcggcagc ggggttgtgc
     4201 acggcttcga gtgccagatc gcaataccgt cgctgatcag gttgaggacg accagaacaa
     4261 gacatgtaag ggccaaggcc caggctag
//
LOCUS       DASWOG010000448         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_120579,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000448 DASWOG010000000
VERSION     DASWOG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120579"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VGB93_09300"
     CDS             <1..943
                     /locus_tag="VGB93_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEY0146328.1"
                     /translation="ATAHARKRFELIVRPRPNAFSLLFIMRGSILPMIAPRVLCVLAV
                     SAAVVVWHHFAPTLFSNVSAAPFTLLGLALSIFLGFRNNACYERWWEGRKQWGQLAAE
                     ARNLTREFLVLLPCDAALQRRCAHRLVAFAHALRNQLRDGDDGKTLDWLPKDETAAVT
                     GSHCQPDAIQRAQAVEFAALRQAGKLADIPYSILSRHLQALTEVQTACERLHTTPTPF
                     TYTLLLHRTAWLFCLLLPFGLVGTVGLATPVLTAILAYSFFGLDALGEELEEPFGGTQ
                     NALPLDALVRNIEIAVGEALGDATLPEPLKPKNFILD"
     gene            complement(1025..>2397)
                     /locus_tag="VGB93_09305"
     CDS             complement(1025..>2397)
                     /locus_tag="VGB93_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0146329.1"
                     /translation="AGYEVSSEPAILICLIVKEAVANAIRYSHPAGVAGLIKIVCERN
                     HDGTFSIEVLDDGVGLPEDFDPTKDGGPGLQFIYGAARRLDARLEFESTSLGLCVRLQ
                     VPRASIEARGATQILVTNEKNIGSATGEGENQATLDAFSAVQRLAAIVESCDDAILSK
                     DLNGIIKSWKAGAQRLFGYAAEEMIGKSVMLLIPEDRHDEEPAILHRIRRGERVDHYE
                     TVRIRKDGRPVEISLSVSPIKDTNGVVVGASKIARDISERKQAQARQEFLTREVNHRT
                     KNLFAIIQSVVNRSFLGKSSIAEAKAALLGRLSSLAQTNVLLMEGDFKGADIADSIRT
                     EMSPYHDRAEIEGPHFMLSAQAAQNIALAVHELATNATKYGALCTPSGSICINWRLVD
                     DEGRQFFVFGWREQGGPEVTAPQRKGFGSTVLEHVMAEYFETRIDFAPSGVVYELKGP
                     FDGIAG"
BASE COUNT          438 a          748 c          677 g          534 t
ORIGIN      
        1 agccacggcg cacgcccgga agaggtttga attgatcgtc cgtccccgcc ccaacgcgtt
       61 cagcctgctc ttcatcatgc gcggctccat cctgcccatg atcgcgccgc gcgttctgtg
      121 cgtgctggcc gtctcggccg ccgtcgtcgt ctggcatcat tttgcgccaa cgctgttcag
      181 caatgtctcg gcagccccct ttacgttgct cggcttggcg ctctcgatct tcctcggctt
      241 tcgcaacaac gcctgttacg agcgctggtg ggagggtcgc aagcaatggg ggcagctcgc
      301 cgccgaggcg cgcaacctga cccgcgagtt tctggttctg ctaccctgcg acgccgcatt
      361 gcagcggcgc tgcgcacatc ggctcgtcgc tttcgcccat gcgctccgca atcaattgcg
      421 cgacggcgat gacggcaaga cgctggattg gctgccgaaa gacgagacgg cggcggtcac
      481 cggaagccat tgtcagccgg acgccatcca gcgcgcgcag gcggtggaat ttgcggcgct
      541 tcggcaggcc gggaagctcg ccgacatccc gtattcgatc ctgtcgcgcc accttcaggc
      601 tttgaccgag gtccagaccg cttgtgaacg gctgcatacg acgccgacgc ccttcactta
      661 cactttgctc cttcaccgga cggcctggct gttctgcctt ctcctgccct tcggccttgt
      721 cgggacggtc ggcttggcca caccggttct gaccgcgatc ctcgcctatt ccttcttcgg
      781 tctcgatgcc ctgggtgagg aattggaaga gccgttcggc ggcacgcaga atgccctgcc
      841 gctcgatgca ctcgtccgca atatcgagat cgccgtgggc gaagctctgg gcgacgcgac
      901 tttgccggaa ccgctgaagc cgaagaattt cattctcgac tgagtgcctt ctattttcct
      961 gcattaagtc tggttgccga cccttcggtt gggtggaccg cccatcgcct tgagactcag
     1021 ctttttaccc ggcgataccg tcgaaagggc ccttcagctc gtaaacgacg ccggacggcg
     1081 caaagtcgat ccgggtctca aaatattccg ccatgacgtg ttcgagaacg gtgctgccga
     1141 agccctttcg ctgcggcgct gtcacctcag ggccgccctg ctcgcgccag ccaaagacaa
     1201 agaattggcg accctcatcg tcaacgagcc gccaattgat gcatatgctt cccgagggcg
     1261 tgcaaagcgc cccgtatttg gtggcattgg tcgccagttc gtggacagcg agagcgatgt
     1321 tctgcgccgc ctgcgccgat aacataaaat gcggcccctc gatttcggcg cgatcgtgat
     1381 aggggctcat ttcagttcga atgctatccg ctatatccgc gcctttaaaa tcgccctcca
     1441 tcagcagaac gtttgtttgc gccagcgaac taagacgacc gagcaacgcg gctttggcct
     1501 ccgcgatcga gcttttgccg aggaagcttc tgttgacgac tgactgaatg atcgcgaata
     1561 aatttttcgt gcgatgattt acttcgcggg tcagaaattc ctgccgcgcc tgggcttgct
     1621 tacgttcgct gatatcgcgc gcgatcttcg atgcgcctac gactaccccg ttcgtgtctt
     1681 tgatcggaga aacactaagc gaaatttcaa ccggcctgcc gtctttgcga atccgaaccg
     1741 tttcgtaatg atcgacccgc tcgccacgac ggatgcgatg tagaattgcc ggttcctcgt
     1801 cgtggcgatc ctctgggatg agaagcatga ccgacttgcc gatcatctct tcagcggcat
     1861 agccgaagag cctctgcgca ccagctttcc aactcttgat gatgccattg agatctttgc
     1921 tcaagatagc gtcgtcgcag gattcgacga tggccgcgag ccgctgcacc gcggagaagg
     1981 cgtcaagcgt ggcttgattt tccccttcgc cggtggctga gccgatattc ttttcattgg
     2041 taacaagtat ctgcgtggcg ccgcgcgctt caatcgatgc gcgtggtact tgtaaacgca
     2101 cgcaaagccc caaacttgtc gattcaaact ctagacgcgc atcgagtctg cgggccgcgc
     2161 cgtagatgaa ctgcaagccg gggccgccat ccttcgtcgg atcgaaatcc tcgggcagac
     2221 cgacgccatc gtcgagaact tcgatggaga aagtgccatc gtggtttcgt tcgcaaacga
     2281 tcttgataag gccggcaacg ccggcggggt gagaatagcg gatcgcattg gcgaccgctt
     2341 ccttgacaat gaggcaaatt aaaatcgccg gctcgctgga cacttcatag cctgccg
//
LOCUS       DASWOG010000449         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_322766,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000449 DASWOG010000000
VERSION     DASWOG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322766"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB93_09310"
     CDS             <1..822
                     /locus_tag="VGB93_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146330.1"
                     /translation="TKPGFCLDTPETGVTCSGVTGINFRGGGPKFSAGGIRFQKASWC
                     AIKVAQFDNFSDEAIVIDAGMACVIEDVLTTNVLLNRARPKPAGAITLAGTDHYLNRV
                     ESNTSLHALSSDGPERIIAAFLIRGANHFISNCVGEISDVGYIVEGLYHRFTGCRGDR
                     NFGEGFIINCKLTTFSSCTALDNNHDPKAKASGFLILGPSNTFASCIAACNRLPSLQL
                     YGFDEHNPIGDVDRDTLYAACRSLNHQIAAQRCFTAKGKDLTFADVATRSGAEIR"
     gene            complement(838..2043)
                     /locus_tag="VGB93_09315"
     CDS             complement(838..2043)
                     /locus_tag="VGB93_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0146331.1"
                     /translation="MLAPSLEETKVVEKTGQDDFYTSPTAVVYIFGAIDTLVPFVYIL
                     NIFSHINYWHGATLAIKLLLCCLYFRYISISLTALVSLVVLTAIILIASIPGGSQISD
                     YIRIFGFVAHVFVTLAIIRRENLEKYLRACVGVLFVTTLIYLLAAALGQINITFGRYS
                     YFHGSHPNLGSEIIAMSIVLAACVLSWLPFLIVTLPSLYAIHLMEGRAALIVALIAVG
                     AKFIYDQWDNKKRLALFCGLFVCAGAALVLVSSKAASVFNSIFLLHDKYRGLGSGFVG
                     RDQLWSTALEWFRASPIIGNGAGFFDRNGVEIHNFFLFGLSEFGLLSLIIYGMIFYLF
                     YDLYRTDKKWFVCFLGIPVFWMFNDRFFNINPYPFLFYVVLFAHANKARLGIGALPVT
                     QTGLVPHAR"
     gene            complement(2070..>2671)
                     /locus_tag="VGB93_09320"
     CDS             complement(2070..>2671)
                     /locus_tag="VGB93_09320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0146332.1"
                     /translation="GKIDVLPYCYDDVFFQPGPPPLRPTNRPVRFLFLGQAGPRKGIH
                     LLLKAFARLPRNAATLTVVGQLQVPAATFRQYSDLVTLISTVPRSAVADYLRESDCLV
                     FPTFFEGSPITIYEALACGCAIIQSKNSNLDAIPMAGMALERLDEDSVYAALMYAIEN
                     RDVLQSWQQAAPAIAEGFTIAAYQKAVMAYLDKMLPAKG"
BASE COUNT          640 a          755 c          772 g          504 t
ORIGIN      
        1 accaaaccag gtttctgcct cgatacgccg gaaacgggcg tcacttgctc cggcgtcacc
       61 ggcatcaact ttcgtggcgg cggccccaaa ttctccgcag gcggtatccg ctttcaaaag
      121 gcaagctggt gcgcgatcaa agtcgcgcaa ttcgacaatt tctctgacga ggcgatcgtc
      181 atcgacgccg gtatggcctg cgtgatcgag gacgttctga cgacgaacgt cctgctcaat
      241 cgcgcgcggc ccaaacccgc cggcgccatc acgcttgccg gaaccgacca ttatctcaac
      301 cgtgtcgagt cgaacacctc gcttcacgcc ctgagttcag acggcccgga gcgcatcatc
      361 gccgcctttc tcattcgcgg cgccaatcat ttcatcagca attgtgtcgg cgaaatttcc
      421 gatgtcggct acattgttga aggactttat catcgcttca ccggctgccg cggcgatcgc
      481 aattttggcg aaggcttcat catcaattgc aagctgacga cattttcaag ttgcaccgcg
      541 ctcgacaaca atcacgaccc gaaagcgaag gcgtcgggct tcctgatcct tggcccatcg
      601 aacacgttcg caagctgcat cgccgcctgc aaccgcctgc cgtcgctcca gctttatggc
      661 tttgacgagc acaatcccat cggcgacgtc gatcgcgaca cgctttatgc cgcctgccgc
      721 agcttgaacc accagatcgc ggcgcagcgt tgcttcacgg cgaaaggcaa ggatttgaca
      781 ttcgccgatg tcgcgacgcg atccggcgcg gaaatccggt aagccggatt tcccgaatta
      841 tctcgcgtgg ggcactaaac cggtttgcgt cactggcagc gcgccgatcc ccaggcgcgc
      901 tttattggcg tgggcgaaaa gcacgacgta aaacaggaat ggataaggat tgatattgaa
      961 gaagcggtcg ttgaacatcc agaagaccgg gatgccgaga aaacagacga accacttctt
     1021 gtcggtgcga tatagatcgt agaacagata aaagatcatc ccgtagatga tcagcgacag
     1081 cagcccgaat tccgacaggc cgaataaaaa gaaattatga atctcgacgc cgttgcgatc
     1141 gaagaatccg gcgccgttgc cgatgatcgg gctcgccctg aaccattcca acgccgtgct
     1201 ccaaagctgg tcgcggccga cgaagccgga accgaggccg cggtatttgt catgcagcag
     1261 gaaaattgaa ttgaagacgc tggccgcttt actcgacacg aggacgagcg ccgccccggc
     1321 acagacaaac aacccgcaaa acaatgcgag gcgcttcttg ttgtcccatt gatcgtagat
     1381 aaatttggcg ccgaccgcga tgagcgcgac gatcaacgcg gcgcggcctt ccatcaagtg
     1441 gatcgcgtaa agtgacggca gcgtcacgat gaggaacgga agccacgaca gcacgcaagc
     1501 ggcaagcacg atcgacatgg cgatgatctc ggagccgaga ttcggatgcg agccatggaa
     1561 gtaggaatag cggccgaaag tgatgttgat ctggccgagc gcggccgcga gcagatagat
     1621 gagcgtagtg acaaagagca cgccgacgca agcccgaagg tatttttcga gattctcgcg
     1681 gcgtatgatc gccagcgtga cgaagacatg cgccacgaat ccgaaaatcc tgatgtagtc
     1741 ggagatttgc gagccgcccg gaatcgacgc gatcaggatg atcgccgtca ggacaacaag
     1801 cgataccaga gcggtgaggc tgatcgagat gtatctaaaa taaaggcagc acagcagaag
     1861 ctttatcgcc agcgtggcgc cgtgccaata attgatatgc gaaaaaatgt tgaggatata
     1921 aacaaacggg acaagcgtat cgatcgcgcc gaaaatataa acgacggcgg tcggcgacgt
     1981 ataaaaatcg tcctgcccgg ttttttccac gactttggtt tcttctagcg acggtgcgag
     2041 caaatggttc tccgggcctc ggcgggcgat tatccctttg ctggtaacat tttatcgaga
     2101 taggccatta ccgccttctg atacgcggcg atagtaaaac cttcggcaat cgcgggtgcg
     2161 gcctgctgcc acgattgcag gacgtcacga ttctcgatcg catacatcag cgccgcgtaa
     2221 acactgtcct cgtccagccg ttcaagcgcc atcccggcca tcggaatggc gtcgagattc
     2281 gaatttttcg attgaatgat ggcgcagccg caagccagcg cctcatagat cgtaatcggc
     2341 gagccttcaa agaaggtcgg aaagaccagg cagtcggact cgcgcagata gtcggccacc
     2401 gccgagcgtg gcacggtgga gatcaaggtc acgagatcgg aatattggcg gaaggtcgcc
     2461 gccggcacct gcaattggcc gacgacggtc agcgtcgcag cattgcgcgg taggcgcgcg
     2521 aacgccttga gcagaagatg gatgcctttg cgtggccccg cctggccaag gaagaggaac
     2581 cgcaccgggc gattggtcgg ccgtagcggg ggcgggcctg gctggaagaa gacatcgtcg
     2641 tagcaataag gcagcacgtc gattttgccg g
//
LOCUS       DASWOG010000450         6022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_120650,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000450 DASWOG010000000
VERSION     DASWOG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120650"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGB93_09325"
     CDS             <1..547
                     /locus_tag="VGB93_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0146333.1"
                     /translation="VAGIVEKTKTYKPGNPVDPQTTMGTLISKAHLDRVLHYIELGIS
                     EGATLALGGGRPSDPALAAGHFVNPTIFINVDASMQIAREEIFGPVLSVLRWSDEAKM
                     LEDVNDVDYGLAAAIYTKDLATAHRTAAAVEAGYIWVNNSATHYLGAPFGGYKLSGIG
                     REEYSGEIESFTQLKNISITL"
     gene            complement(629..1885)
                     /locus_tag="VGB93_09330"
     CDS             complement(629..1885)
                     /locus_tag="VGB93_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146334.1"
                     /translation="MPTDQMIDYVPDNRVDSGAGVNAVRIAGIGMFVLLLATYSLNAM
                     DRQIFPLLLNDVRHQYGFSLSSGGLLSTVFTFGMACAGLPTGWLMAHLSRRATVQIGI
                     VIFSAATVLTALAPGFWTMMFCRGLTGIGEAMQLTALVAIAGSYFAKHRSLAVGAVNT
                     SFGIGAIIGPALGGAILASYHTWHAPMFTFGALGFIAIALIAVFVRPWLSEAAANAST
                     EGAHVSQGFETLLNRHTILLSVLSLIGGLIIYGYLGMYPSYLRTHLGYSPQAAGNVMA
                     FFGFGVLASIAGGAIGDRLPARVLLPAAFLGGGAIGFGLFHGPSNFAAQAGLSFLWGL
                     IVSGTVYVNLAAYHVKAVVPRLAARSSGVFVTTLYASSSVAGYVFGALATWLGWTRAA
                     DLQIVCLCVAGALLAACLGSSRSASR"
     gene            complement(1908..2921)
                     /locus_tag="VGB93_09335"
     CDS             complement(1908..2921)
                     /locus_tag="VGB93_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146335.1"
                     /translation="MNKSLSACAIAIGLLFAGLDSASSANAPLEKLTVNVFPGGFNWP
                     LFVARDKGFFARQGLEVTVQPTTGSVAQMSGLASGKFDIAMTAVDNIVAYVEGEGDKS
                     VGKQPDFFGFMGSDSGFLSLVSVPAVKSIGELRGKTLLVDARSTGYAFVLYDMLRHAG
                     FKSTDYQVVPAGGMVQRWAAMQKNSSAATLLSTPFNILAKDKGFHQLSWATQVIGPYQ
                     GNVAATKRAWAAAHHQEVLGYIRAYAVAIDWLYKPGNRDEAIRILRANLPNMPEKLAE
                     QTYGELLDPKAGFFRSAKVNMDGLRTVLRLRSTYGSGPKLDDPARYYDASYWADAMKA
                     PAK"
     gene            complement(2948..3817)
                     /locus_tag="VGB93_09340"
     CDS             complement(2948..3817)
                     /locus_tag="VGB93_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0146336.1"
                     /translation="MLPTERIDYVPIAGRKKLHLPDGKRLIVWVIVNVEEWNARETMP
                     RTVLTPPAGGSPTPDIPNWAWHEYGNRVGFWRMLAVFDDLKIPAVLAINGSAINAYEP
                     IARAALDRKWEFIGHGFTQKNMQKVDDESEDIRKTTDAIKAFTGRPPRGWLGPGLTET
                     WNTPDLLKEIGYDYVCDWVLDDQPVVLRTAHGPLVNVPYTQECNDVAMMLIQHHKASE
                     YRDRAIDQFEQIYADSEDSARVMALVVHPYIMGAPHRLRYFREALQHIAAKPDVAWWT
                     GEQILDWYAGQSA"
     gene            complement(3819..4526)
                     /locus_tag="VGB93_09345"
     CDS             complement(3819..4526)
                     /locus_tag="VGB93_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0146337.1"
                     /translation="MLAEKAYLFTPQIAALPVAGATSRFPVRRLYCVGRNYVSHIREM
                     KEGDERDPPFFFQKPADALVFDGGTVPYPPATTDFQYEVELVVAIGTAGAFIAVDDAL
                     NHVYGYAIGIDMTRRDLQREARERQLPWEPGKSFDHSAPCGPLFPKKDVGDIASSAIS
                     LSVNGIPHQQADLKQMIWNVPEIIANLSKQYRLEPGDLIFTGTPAGVGPVEVNDRIEA
                     TIGGLGSLSITITSKEV"
     gene            4687..5418
                     /locus_tag="VGB93_09350"
     CDS             4687..5418
                     /locus_tag="VGB93_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0146338.1"
                     /translation="MSQASDAAGSGALAQRKRTGALAVTESIRSALLDGNLGKGRINE
                     VHLASTLGVSRTPVRAALQMLAGEGLLDHAPNRGFAVRSFSLDEIGDAFEVRALNEGL
                     ACRLAAERGLTLAQDDTIREVLGRGDRLLSGTVDLVECRRGYAEINTQFHATLLEAAG
                     SRLIVDVLKLCNRVPQTTTHNVMAFNLDDVRQRHTEHHAIYKAVALREPQSAQAQMRE
                     HILHVKNSLVRSALAERIASARAGE"
     gene            complement(5546..>6022)
                     /locus_tag="VGB93_09355"
     CDS             complement(5546..>6022)
                     /locus_tag="VGB93_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146339.1"
                     /translation="AQALDEGSIDGFWANGLGAEYAVRSGKGDIVLDVRRGLGPSVAF
                     HYTFPAVAASDRLIAAEPDLVERAIRAIVRAQRMLRADISLASVVGRKLFPKAEAEYT
                     EAVVTRDLAYYSAEISSQTVDGIIRFSQACGLLKGTPSRDDIVAMPFSRLWGEAGI"
BASE COUNT         1253 a         1747 c         1853 g         1169 t
ORIGIN      
        1 ggttgccggc attgtggaaa agacgaaaac ctacaagccc ggcaatcccg tcgatccgca
       61 aacgacgatg gggacgctta tttccaaagc gcatctcgat cgcgtcctgc attatatcga
      121 gctcggtatt tcggaaggcg cgacgcttgc tttgggcggg ggccggccga gcgatccggc
      181 tttggccgcg ggtcatttcg tcaatccgac gatcttcatc aacgtcgatg cctcaatgca
      241 aatcgcacgc gaagaaatat tcggtccggt cctctccgtc ttgcgctgga gtgacgaggc
      301 caaaatgctc gaagacgtga atgatgtgga ttatgggctg gcggcagcga tctatacgaa
      361 agatcttgcg acggcgcacc gcacggcggc agcggtggaa gcgggttata tctgggtcaa
      421 taattccgcc acgcattatc ttggcgcccc attcgggggc tataaacttt ccggaatcgg
      481 gcgcgaggaa tacagcggcg agattgaatc cttcacgcag ctgaagaata tcagcatcac
      541 gctctagggc gcggtatttc agccagctaa agaacacgcc ggccgtcgcc gcgggcaatt
      601 gctaacatcg cggtggtcgc tcggcgaatt atctcgaagc cgagcgactg cttccgaggc
      661 atgcggcgag gagtgcgccc gcaacgcaca ggcatacgat ctgcaaatcg gcggcgcgcg
      721 tccatccgag ccaggttgcc agggcgccga aaacatagcc ggcgactgac gacgatgcat
      781 aaagcgtcgt cacaaaaact ccggaactcc gcgccgccag tcgtggaacg acggctttga
      841 cgtgataggc ggcaaggttg acatagacgg tgccgctgac gatcaggccc cagagaaaag
      901 acagtccggc ctgcgcggcg aaatttgaag gcccgtggaa aagaccgaag ccgatcgcgc
      961 cgccgccgag aaacgccgcg ggcagcagaa cgcgcgctgg cagacgatcg ccgatcgcgc
     1021 cgcccgcgat ggacgcgaga acgccaaagc caaagaaggc catgacattg ccagccgctt
     1081 gcggcgaata gccgagatgc gtccgcaggt aacttggata catcccgaga taaccgtaga
     1141 tgatcaatcc gccgataagg ctcaggacgc taagcagaat cgtgtgtcgg ttcagcaagg
     1201 tttcaaagcc ttggctgacg tgggcgccct ctgtagatgc attggcagcc gcttcgctca
     1261 accaggggcg gacgaagacc gcgatcaacg caatggcgat gaagccaagg gcgccgaagg
     1321 taaacatcgg cgcgtgccag gtatgatacg aggcgagaat agcgcccccc aacgccggac
     1381 cgatgatggc gccgatgccg aaagatgtgt tcacggcgcc gacggcaagc gagcggtgtt
     1441 tggcgaaata gcttccggcg atggctacca gtgccgtgag ttgcatggct tcgccgattc
     1501 cggtcaagcc ccggcaaaac atcatggtcc aaaagcccgg cgccagcgcc gtcaggacgg
     1561 tcgccgccga aaaaatcaca atgccgatct gcacggttgc gcgtcgcgac agatgtgcca
     1621 tcagccagcc ggtcggtagc ccggcgcagg ccattccgaa cgtgaagacc gtcgaaagca
     1681 ggccaccact cgaaagtgaa aacccgtatt ggtgccgaac gtcgttcagc aaaagcggga
     1741 agatctgccg atccatcgca ttgagcgaat aggtggcaag aaggagcaca aacatgccga
     1801 tgccagcgat ccgcacggca ttcacgcctg cgccggagtc aacgcggttg tctgggacat
     1861 aatcaatcat ctggtccgtc ggcatgatgg cgctctttca gaggtttcta tttcgcgggg
     1921 gctttcatgg cgtctgccca atagctcgcg tcgtaatagc gagcggggtc gtcaagcttc
     1981 ggcccgctgc cataggtgct ccgcaggcgc agcactgtcc gcagtccgtc catattcact
     2041 ttggcactcc ggaaaaaacc ggccttggga tcaagcaatt cgccataggt ctgctcggcg
     2101 agcttctccg gcatgttggg cagattggcg cgcagaatgc ggatcgcttc gtcgcggttg
     2161 ccgggcttgt aaagccaatc gatcgcgact gcgtaagcgc ggatatagcc cagcacctcc
     2221 tggtgatgcg ctgcggccca ggcgcgcttg gttgcggcga cgttgccctg ataaggcccg
     2281 atcacctgcg tcgcccacga caattgatgg aagcctttgt ccttggcgag aatgttgaag
     2341 ggcgtcgaga gaagcgtggc ggcgctgctg ttcttttgca tcgctgccca acgctgaacc
     2401 atgccgcccg cgggaacgac ctgatagtcc gtgcttttga agccggcgtg ccgcagcatg
     2461 tcataaagga cgaaagcgta gcccgtgctg cgggcatcca ccaaaagcgt tttgccgcgc
     2521 agttcgccaa tggatttgac cgccggaacg ctgaccaggc tgaggaagcc gctgtcgctt
     2581 cccatgaagc cgaaaaagtc cggttgtttg ccgactgatt tgtcgccttc gccctcaaca
     2641 taggcgacga tattgtccac ggccgtcatg gcaatatcga acttgccgga tgcaaggccc
     2701 gacatttgtg ccaccgatcc cgtcgttggc tgcaccgtga cttcgaggcc ttgccgggcg
     2761 aaaaaacctt tgtcgcgggc gacgaacagc ggccagttga agccgccggg gaagacgttg
     2821 actgttagct tttcgagcgg cgcattggcc gagcttgccg aatcgaggcc cgcgaagaga
     2881 agcccgatgg cgatcgcgca ggcagataga cttttgttca ttagatatgg tctcgtactt
     2941 gtgagagtca ggcgctttgg ccggcgtacc agtcgaggat ttgctcacca gtccaccacg
     3001 cgacatcggg cttggcggcg atatgctgca aggcttcacg gaaatagcgc agccgatgcg
     3061 gcgcacccat gatgtatgga tgtacgacca gcgccatgac gcgcgcgcta tcctcggaat
     3121 cggcatagat ctgctcgaac tgatcgatgg cgcggtctcg gtattcggag gctttgtgat
     3181 gctggatcag catcattgcc acgtcattgc actcctgcgt atagggcaca ttgacaagcg
     3241 gaccgtgcgc ggtgcgcagg acgacgggtt ggtcgtccag gacccagtcg cagacgtagt
     3301 cgtagccaat ttctttgagc aggtcgggcg tattccaggt ttcggtcagg cccggcccaa
     3361 gccagccgcg cggcggccgt ccggtgaaag ctttgatcgc gtccgttgtc ttacggatat
     3421 cctcgctttc gtcgtcgacc ttttgcatat tcttctgcgt aaagccgtgg ccgatgaact
     3481 cccatttgcg atcgagtgca gcgcgcgcga ttggctcgta ggcgttgatc gccgacccgt
     3541 tgatcgcaag cacggctgga attttaagat cgtcgaagac agccagcatg cgccagaatc
     3601 cgacgcgatt gccgtattcg tgccacgccc aattcgggat atccggtgtc ggcgacccgc
     3661 cggccggcgg agtcagcaca gtacgcggca tcgtttcacg cgcgttccat tcctctacat
     3721 tcacgatgac ccagacgatc agacgtttgc cgtcgggaag gtgaagcttt ttacggccgg
     3781 cgatggggac gtagtcgatg cgttcagtag gcagcatctc aaacctcctt cgacgtgatc
     3841 gtaatggaca gcgacccgag gcctccgatt gtcgcttcga tccgatcatt cacctccacg
     3901 gggccgacgc cggccggcgt gcctgtgaaa atgagatcgc ccggctcaag ccgatattgc
     3961 ttggacagat tcgcgatgat ctctgggacg ttccagatca tctgcttcag atcggcttgc
     4021 tgatgcggga tgccattcac cgaaaggctg attgcactcg atgcgatgtc gccgacatct
     4081 ttttttggga agagcgggcc gcacggcgct gagtgatcga aagactttcc cggctcccac
     4141 ggaagctgac gctcgcgcgc ttcgcgttgc agatcgcgcc gcgtcatgtc gataccgatt
     4201 gcgtagccat agacatggtt gagggcgtca tccacagcga tgaatgcacc cgcggtgccg
     4261 atcgcgacga cgagttcgac ctcgtattga aagtctgtcg tggctggcgg gtagggcacc
     4321 gtgccgccat cgaagacgag ggcatcggcc ggcttttgaa agaaaaacgg gggatcgcgc
     4381 tcgtcaccct ccttcatctc acgaatgtgc gagacataat tgcgtccgac gcagtaaagc
     4441 cggcggactg gaaatcggga cgtggcgcca gcgaccggca gggcggcaat ctggggtgtg
     4501 aatagatagg ccttttcggc gagcatcttc cctctcggcg gcttgatgaa gtcaaactga
     4561 atacacattg gcattattcg tgccaaagtg tgtacacttt agcttgattg ggccgtgatt
     4621 ggatcggctg cggccgtcga gatatgacag agacggaaat gtcagcctca agcggacgtc
     4681 gtggatatga gccaggcatc tgatgccgcc ggaagcggcg ccctcgcaca aagaaagcgc
     4741 accggcgctc tggcggtaac ggaatcgatc cgcagcgcgc tgctggatgg aaatctcggc
     4801 aaggggcgga tcaacgaggt gcatctcgcg agtactttgg gtgtctcgcg cactccggtg
     4861 cgcgccgcgc tccagatgct tgccggggag gggcttctcg accatgcgcc aaatcgcggt
     4921 ttcgcggttc gctcgtttag ccttgacgag atcggcgacg ctttcgaagt gcgggcgctc
     4981 aacgagggat tggcctgtcg cctcgcggcg gagcgcggcc ttacgctggc tcaggacgat
     5041 acgatccgcg aagtcctcgg ccgcggcgat agactccttt ccggcaccgt cgatctcgtc
     5101 gaatgccggc gcggttatgc ggagataaat actcagtttc acgcgacgct gctcgaggcc
     5161 gctggttcga gattgatcgt cgatgttcta aagctctgca atcgagttcc gcagaccacg
     5221 acgcataacg tcatggcctt caatctggac gacgtgcggc agcgccatac agaacaccat
     5281 gctatttata aagctgttgc cttacgtgag ccgcagagcg cgcaggcaca gatgcgcgag
     5341 cacatcctcc atgtgaagaa ctctttggtg agatcggcgc ttgccgaacg catcgcatcg
     5401 gcgcgagctg gcgagtagcg ctttcgcgcg gccgacgatt ccgatttttg gatttcgctc
     5461 ctgccgcaga caggcagttc ggcgtcacga aataggccag cgtagcagtg gccgcgctgt
     5521 gttgcgcgcg gccaccgggt ggactctata tccccgcttc gccccagaga cgcgagaacg
     5581 gcatcgcaac gatgtcgtcg cgggaaggcg tacccttgag aaggccgcaa gcttgcgaga
     5641 aacggatgat cccgtcgacc gtctgggacg aaatttccgc tgaataatag gcaagatcgc
     5701 gcgtcacgac ggcttcggtg tattcggctt cggctttcgg gaacagcttc cgtccgacca
     5761 cactcgccaa gctgatatcg gcacggagca tacgctgggc ccgaacaatc gcacgtatgg
     5821 cgcgctcgac gagatcaggc tccgcggcaa tcaagcggtc gctcgccgcg acagcgggga
     5881 aagtatagtg gaaagccacc gacgggccga ggccgcgccg gacatcgaga acgatatcgc
     5941 ccttgccact tctcaccgca tattcggcgc cgaggccgtt ggcccaaaat ccgtcgattg
     6001 atccctcatc cagcgcctgc gc
//
LOCUS       DASWOG010000451         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_358588,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000451 DASWOG010000000
VERSION     DASWOG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358588"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGB93_09360"
     CDS             complement(<1..280)
                     /locus_tag="VGB93_09360"
                     /inference="COORDINATES: protein motif:HMM:NF045295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0146340.1"
                     /translation="MLNRYGSAGAALAALPRLMPRGRAAKVASVEDIESEFDAAARSG
                     ARFVAMGEPDYSPSLGNIDAAPPLIAVRGNLSVFQRPAVAIVGSRNASA"
     gene            complement(379..990)
                     /gene="plsY"
                     /locus_tag="VGB93_09365"
     CDS             complement(379..990)
                     /gene="plsY"
                     /locus_tag="VGB93_09365"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0146341.1"
                     /translation="MAVLSSVVALIFGYFCGAIPFGLLLTRLAGTADLRSLGSGNIGA
                     TNVLRTGRKDLAAATLLLDALKGLAAVLIVGAFAPGLLPLFAAAGAFLGHVFPVWLGF
                     RGGKGVATFLGCLFGLDWHIALIFVALWLAVAAATRYSSAGALTASAATPIALLVDGR
                     RDVAVLYALLTLLLWYMHRANIARLSAGTESKIGGPKAVGDNA"
     gene            complement(1058..>1691)
                     /locus_tag="VGB93_09370"
     CDS             complement(1058..>1691)
                     /locus_tag="VGB93_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0146342.1"
                     /translation="VEGWSLAERLRREFESVGFFLSGHPLDAYDSVLARLRVRRWSDF
                     ARSVKLGASAADRLAAVVLDRQERRTKSGNKMGIVQLSDQSGQYEAILFQEGLNQYRE
                     LLEKGAAVLVSLQANVEGEDVRARIVSVEPLDQAASRHQKGLRIFLRDIAPLPSIARG
                     LNGRGDGEVSLMLMTDSGEVEIKLPGKYPSNAQVASTLKAIPGIVNVEHI"
BASE COUNT          385 a          550 c          501 g          255 t
ORIGIN      
        1 cggcggaggc attgcgcgag ccgacgatgg cgaccgctgg ccgctggaac acagacagat
       61 ttccgcgcac ggcgatgagc ggcggcgctg catcgatatt gccaagactc ggcgaatagt
      121 ctggctcgcc catcgcgacg aaacgcgcac cggagcgtgc ggcggcgtcg aattcgctct
      181 cgatgtcttc gaccgaagcg accttggccg cccgcccgcg cggcatcagc cgaggcaggg
      241 ccgcaagcgc cgcgccagcg ctgccatagc gattaagcaa ttcccggaac gtccgcagct
      301 cgatattttc acagcgccac agtcgcagtc aatcgaagcg ctgggcgtcg gacagtttgt
      361 tgcctgccgc gccagcactc atgcgttatc gccaaccgcc ttcggcccgc caatcttgga
      421 ttcagtcccc gcagagaggc gagcgatatt ggcgcgatgc atataccaga gcagcaacgt
      481 caggagcgca taaaggacgg cgacatcgcg ccgcccgtca accaataatg caatcggtgt
      541 tgccgcgctg gccgtaagcg cgcccgcaga agaatagcgt gtcgccgcag cgaccgcgag
      601 ccagagcgca acgaagatca aagcaatgtg ccagtcgagg ccaaaaaggc aaccaaggaa
      661 cgtcgcgacg cccttaccgc cacgaaagcc gagccaaacc gggaagacat ggccaagaaa
      721 agctccggcc gcggcgaaga gcggcagcag accgggcgca aaagcgccga caatcaagac
      781 cgcggcaagc cctttcagcg catcgagcaa caacgtcgcc gcagcgagat ccttgcgccc
      841 ggtgcgtagc acattcgttg cgccgatatt accggagccg aggctgcgca aatctgcggt
      901 cccagcgagg cgcgtcagca gaagcccgaa tggaattgcg ccgcagaaat agccgaagat
      961 aagggctacg acagaactca acacggcgat ctcaccccga aaacatgcgc gtcaaactag
     1021 ccggacagca ccagcgcggc aaccggcgcg gcccgcgcta gatatgctca acattgacaa
     1081 ttccgggaat ggccttcaaa gtcgaggcga cctgtgcgtt tgacgggtat tttccgggga
     1141 gctttatttc gacctcgccc gaatccgtca ttagcatcag cgagacttcg ccgtcgcccc
     1201 tgccattgag accgcgcgcg atcgacggca gcggcgcaat atcgcgcaaa aatatgcgta
     1261 ggcccttttg gtgacggctg gccgcctgat ccaaaggctc gacagagaca attcgcgccc
     1321 gcacgtcctc gccctcgaca ttggcttgca gcgaaaccag aacggcggcg cctttttcca
     1381 gcagttcgcg atattgattg agcccttcct gaaacaggat ggcttcgtat tggccggatt
     1441 gatccgacaa ttgcacgata cccatcttgt tgccggactt tgtgcgccgc tcctgccgat
     1501 cgagcacgac ggcagcaagg cggtccgccg ccgaagcccc cagcttcacg gagcgggcga
     1561 aatcgctcca gcggcgcacg cgcagccgcg ccagcacgga atcataagca tcgagcggat
     1621 ggccggatag aaagaagccg acggattcga attcgcgccg caaccgctcg gccaacgacc
     1681 agccttcgac c
//
LOCUS       DASWOG010000452         7654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_120673,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000452 DASWOG010000000
VERSION     DASWOG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120673"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VGB93_09375"
     CDS             complement(<1..1014)
                     /locus_tag="VGB93_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0146343.1"
                     /translation="MKCRRVISICAGIAGAWLYAGAGFAEDASTLAKNPNLWPAPGRD
                     LALTRHSELNQINTKNISHLQLAWDQSTGALRGHEGQSLVVDVDGTPMMYIFSAWPNI
                     VQALDLSDPDYPKEVWNYVKKTDRDESAVPRACCDTVNRGGSYADGKVVYLTLDGWLI
                     ALDAKTGKEAWVVKNNYPDKGEDGTPAPLIADGKVFVGFGGDEFAARGSFSAYDLKTG
                     KLVWKKYSTGSDVDVGITADTNKDNQRHSHTAGDLGIHTFPEDNNYKIGGGAAWGWYS
                     YDPELKYIYYGTGNPGLWSPGYRCGEKPLTQAGCNSGKWDNKWSLTIFARKADTGEAV
                     WAYQ"
     gene            complement(1352..3349)
                     /locus_tag="VGB93_09380"
     CDS             complement(1352..3349)
                     /locus_tag="VGB93_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0146344.1"
                     /translation="MFALLSRLSIPRQFTLLGLLAVILTLLGLGMTLKQSYDLALEEK
                     RQDLRSLVESAASSIDFLVQQEKAGTLSTAEAQKRAKALIAAMKFDGNNYFFLYDYDC
                     VLLSINERMRGSIGKNLSHNVDAYGHPTVLPMIESAKAGSPQYNFYYWPRPDNPQPQP
                     KIGYALAIPEWQWVVGAGLYVDDIQATFVASMLKLSAIFVPLFLGLIAIILMMRRSVS
                     KLLRSLTTNMQSLVRGDYETQIVGRTRRDEIGEMAAAVQVFKDNGLRLRAAEVETERL
                     NEAAQATRKSNDEARSEAERRQKSVVAALAGGLGRLAKRDLTSRLEQGFPPEYEQIRA
                     DFNATAESLNQAMRTIIAATVSIDSGSDQIAIAADDLSRRTEQQAASLEESAAALNLI
                     TGTVKKMSADADEAASVARATRQSAEASGQVVARAVQAMDKIKESSNQITQIIGVIDE
                     IAFQTSLLALNAGVEAARAGDTGRGFAVVASEVRALAQRSAEAAKEIKTLISTATVEV
                     DNGVAHVSQTGMALSEITEKVKAMDALIHKISTSSNEQSGGLAEINTAIGSMDTIIQQ
                     NAAMVEESTAAVHGLKDEAKDLMVIVGEFQTGTDAYQRDSSSQNIGRSQRASGPSPQA
                     SRPQAAKQQMSKASPRPLLRTVGSAARKVLSPAQDEAWEEF"
     gene            complement(3615..3701)
                     /locus_tag="VGB93_09385"
     tRNA            complement(3615..3701)
                     /locus_tag="VGB93_09385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3665..3667),aa:Leu,seq:caa)
     gene            complement(3876..5282)
                     /gene="leuC"
                     /locus_tag="VGB93_09390"
     CDS             complement(3876..5282)
                     /gene="leuC"
                     /locus_tag="VGB93_09390"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0146345.1"
                     /translation="MAELRTIYDNIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFE
                     GLRIAGRDVRAPEKTLAVVDHNVPTSDRSEGIKDEESRIQVETLAANAKDFGIDYFHE
                     LDRRQGIVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQSKAKNMRVVVDGRRGPGVFAKDIILAIIGEMGTAGGNGHVIEFAGEAIRDLTMEGR
                     MTVCNMTIEGGARAGLIAPDEATYAFLKDRPRAPKGADWDAARRFWDTLHSDEGAHFD
                     KEIRLDAANLPPIVTWGTSPEDVISITGRVPTPEAAASEAKRASIRRALDYMGLQGGE
                     QISDIAVDRVFIGSCTNGRIEDLRVAAKMVAGKKVSERVNAMVVPGSGLVKEQAEAEG
                     LDKIFKAAGFDWREPGCSMCLAMNPDRLEPGERCASTSNRNFEGRQGFKGRTHLVSPA
                     MAAAAAVAGHFVDVRDWH"
     gene            5455..5856
                     /locus_tag="VGB93_09395"
     CDS             5455..5856
                     /locus_tag="VGB93_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0146346.1"
                     /translation="MRPPVAPYLTVSPAMAAIAFYTTVFGAKQQALMPALDGLRIMHC
                     ELTINGGSIMLADAFPEFGNTRVPVPGEPMTASVSLEFETAEEVDTVFKRAVSLGSKG
                     ETNPTNSFWGTRLATFRDPFGHRWILNAPTA"
     gene            complement(5864..6346)
                     /gene="rplS"
                     /locus_tag="VGB93_09400"
     CDS             complement(5864..6346)
                     /gene="rplS"
                     /locus_tag="VGB93_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0146347.1"
                     /translation="MANIIEELEAAQVAKLVAVRPVPEFQAGDTLIVNVKVKEGERTR
                     VQAYEGVCIARTGGGINESFTVRKISYGEGVERVFPVYSPNIDSIKVVRRGKVRRAKL
                     YYLRDRRGKSARIVEKLETPAQKAAREAANAQAPTVKKAPPVKKATGAIRAPKKGGKG
                     "
     gene            6550..7425
                     /gene="uppP"
                     /locus_tag="VGB93_09405"
     CDS             6550..7425
                     /gene="uppP"
                     /locus_tag="VGB93_09405"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEY0146348.1"
                     /translation="MAGACTEGLDTGFVALGYAKVGFLGIIQGITELLPVSSTAHMRI
                     IPALLGWRDPGSAFSAAMQLAALAAVVSYFWADVKGISEGAVAAVKRRDYEDWSLRFC
                     VWIVLATLPIIIAGSLLSKLLNTCNSPLRSLAAIGVAYIVMGALLAVAEVYSRHKRTL
                     DHVDLKDAMIVGLAQCGALIPGVSRSGSTFTAALFRDLERTEAARFSFLLGIPAIAAA
                     GIKELYELHKAHLDAHGWSILGFGLVVASISAFIAIWGLLRILERFTSWPFVIYRVLL
                     GIFLLAAVAAHWLPN"
BASE COUNT         1456 a         2383 c         2182 g         1633 t
ORIGIN      
        1 ctggtaggcc cagaccgctt cgccggtatc cgccttgcgg gcgaaaatgg tcagcgacca
       61 cttgttgtcc cacttgcccg agttacagcc agcctgcgtc aggggcttct cgccgcagcg
      121 atagccgggg ctccacaaac cgggattacc cgtcccgtag tagatgtact tcagttccgg
      181 atcgtagctg taccagcccc aggccgcacc gccaccgatc ttgtaattgt tgtcttcggg
      241 gaaagtgtgg atgccgaggt caccggcggt atggctgtgc cgctgattgt ccttattagt
      301 atcggcggtg atgccgacat cgacatcgga gccggtgctg tattttttcc acaccagctt
      361 gccggtctta aggtcatagg cactgaagga gccgcgggcg gcgaattcgt caccaccgaa
      421 gccgacgaac accttgccat ccgcaatcag cggcgccggc gtgccgtctt cgcccttgtc
      481 cgggtagttg ttcttgacga cccacgcctc tttgccggtc ttcgcatcaa gcgcgatcag
      541 ccagccgtcg agggtcaggt agacgacctt gccgtcggca taggaaccac cgcggttgac
      601 ggtgtcgcag caagcgcgcg gaacagccga ttcatcacga tcggtcttct tgacgtaatt
      661 ccagacttcc ttcggatagt cgggatcgct cagatcgagc gcctgaacaa tgttcggcca
      721 agcgctgaag atgtacatca tcggcgtgcc gtcgacatcg acgaccagcg actgcccttc
      781 atgaccgcgc agagcgccgg tcgattggtc ccaggcaagt tgaagatgcg agatgttctt
      841 cgtattgatc tggttcagtt cgctgtggcg ggtgagtgcc agatcacgtc ccggcgccgg
      901 ccacagattg gggtttttag caagcgtaga ggcgtcttcc gcgaagcctg ccccggcata
      961 taaccacgcg ccggcaatcc cggcgcagat cgatattacc ctacgacact tcattccgat
     1021 tctccattcc aacgaattat tttcgacaac aaacaacgcc cgcgatcaca aacccgagag
     1081 cttgcgaagc gggcatggcc gaatctgaaa gttgagccgt gaaagcctgg cggccttgtt
     1141 cactctattg cgaaagctat ttcgtctctc gattcaaaga cctcgggcga ggcggaatcg
     1201 aagcgggcgc ctctttttct ctaaccgcgc ttaatgctta ggaaaatccg aaaagctaca
     1261 ggaataattc ccgaccattt cgggatttgg agacggggaa tgttggcaca ctccaagatc
     1321 aacaggcgca gttggcctgc gccgcgaatt gtcagaactc ttcccaggct tcgtcttgtg
     1381 caggtgacag aaccttccgg gcggcgctgc cgaccgtgcg caaaagcggc cgcggtgaag
     1441 cctttgacat ctgctgcttt gccgcctgcg gtctcgatgc ctgtggtgat gggccggagg
     1501 cccgctgcga tcggccgatg ttttgcgaag atgaatcgcg ctggtatgcg tccgtgccgg
     1561 tttgaaactc gccgacgata accatcagat ctttggcttc gtctttcagg ccgtgaacag
     1621 cggccgtcga ttcctcaacc attgcggcgt tttgctgaat gatcgtgtcc atgctgccga
     1681 tcgccgtatt gatctccgcc aggcccccag attgctcgtt cgaggatgtc gagattttat
     1741 gaatcagcgc atccatcgcc ttgacctttt cagtgatctc gctaagtgcc atcccggtct
     1801 ggctgacgtg ggcgaccccg ttatcgactt cgaccgtcgc cgtcgagatc agcgtcttga
     1861 tctctttcgc tgcttcggcc gaacgttgcg cgagcgcgcg cacttccgag gccacgaccg
     1921 cgaaaccgcg tcccgtatcc ccagcgcgcg ccgcttcaac cccagcattg agcgcgagca
     1981 aactcgtctg gaacgcgatt tcgtcgatca cgccgataat ctgggtgatc tggttggacg
     2041 attccttgat cttgtccatt gcctggacgg cccgcgcgac gacctggccc gaggcttcgg
     2101 cggattgccg cgtggcgcgc gcaacactcg cagcctcatc ggcatcggcc gacatcttct
     2161 tcacagttcc ggtgatgaga ttgagcgccg cagcgctctc ttcgaggctc gcggcctgct
     2221 gctcggttcg acgggaaaga tcgtccgcgg cgatggcgat ttggtctgag ccggaatcga
     2281 tactcaccgt ggccgcgata atagtgcgca tcgcctgatt gaggctttct gccgtcgcgt
     2341 tgaagtcggc acgaatctgt tcgtattccg gcgggaaccc ttgttccagc cggcttgtaa
     2401 ggtcgcgctt ggccagacgg ccgagccctc cggcgagcgc tgcaacgacc gacttctgcc
     2461 gacgctcggc ctccgaccga gcctcgtcat tgcttttgcg cgtcgcttgc gcggcctcgt
     2521 tcaagcgttc agtttcgact tccgcagccc taagccgcaa tccattgtcc ttaaaaacct
     2581 ggacggcggc agccatctcg ccgatttcgt cgcggcgcgt gcgtccgacg atctgggttt
     2641 catagtcccc acgcaccagg ctttgcatgt tcgttgtcag cgatctcaaa agcttcgaga
     2701 cggaacgccg catcatcaga atgatcgcga tcaatccaag aaagagcgga acgaagatcg
     2761 cggaaagctt caacatgctc gcgacgaaag tggcttgaat gtcgtcgacg taaagaccgg
     2821 caccgacgac ccattgccat tccggaatcg caagggcgta gccgatcttc ggctgcggct
     2881 gcggattatc gggccttggc caatagtaga aattgtattg tgggctaccg gccttcgccg
     2941 actcgatcat cggaagaacg gtgggatgac cataggcgtc gacgttatga gaaagatttt
     3001 tcccgatcga cccgcgcatg cgctcgttga ttgagagcag aacgcagtcg taatcataca
     3061 aaaagaaata attgttgccg tcgaacttca tcgccgcgat gagggccttg gcccttttct
     3121 gtgcctcggc agtgctcaaa gtaccggctt tttcctgctg gaccaggaaa tctatgctcg
     3181 atgcagcgct ctcaaccaga ctccgcagat cctggcgttt ttcttccaaa gcgagatcat
     3241 aactttgctt caaagtcatt ccgaggccca gaagcgtcag aatgacagca agcagaccga
     3301 gcaacgtgaa ctgcctcgga atcgaaaggc gagaaagcaa agcaaacatt tcagaatctc
     3361 acagcggtac gactcaaaaa tgctgcactg caaccgttaa aaaatcatac gaaacgttga
     3421 attccgcccc ggaattgggt gcttatctgc cggatcacga cggaatattg gtgatgcgca
     3481 aagcgctcca ggagcgaagc acgctcggcc atatgtgctt cgacagcgat attagatgaa
     3541 gcgcccaagc cacagcatga acgcgccaat gctcaatcag aaaacggtct tgccgccgag
     3601 agcattatga tctttggtgc gggcggtcgg aatcgaaccg acactctgtc accagaacca
     3661 gattttgagt ctggcgcgtc taccagttcc gccacgcccg cccggcatgt tccgaaaaag
     3721 ttgtccgact ttttcgatga gaacatgctc agaattgtgc cgaggcgctc acttataaga
     3781 ttggtttgcg gcccgcaaga cgcgagccgc aattttgggt gggtccttgc gcatcactag
     3841 atccgaaaag tccgcaactt ttcggcgcga tacggctaat gccagtcgcg cacgtcgacg
     3901 aaatggccgg ccacagccgc cgccgctgcc atagccgggg agacgaggtg cgtgcggccc
     3961 ttgaagcctt gccggccctc gaaattgcgg ttcgaggtcg aggcacagcg ttcgcccggc
     4021 tcaagacgat cagggttcat cgccagacac atcgagcagc ccggctcgcg ccagtcgaaa
     4081 cccgccgcct tgaagatctt gtccaggccc tccgcctcgg cctgctcttt gacgaggccg
     4141 gagcccggaa cgaccatcgc gttgacccgc tccgagactt tcttgccagc gaccattttg
     4201 gcggcaacgc gcaaatcctc gatccggcca ttcgtgcacg agccgataaa cacccggtcg
     4261 acagcgatgt cgctgatctg ctcgccgccc tgcaggccca tataatcgag cgcgcgccgg
     4321 atcgaggcgc gcttggcctc gcttgctgct gcctcaggcg tcggtacgcg gccggtgatc
     4381 gagatcacgt cctcggggct cgtgccccag gtgacgatcg gcggcagatt ggccgcgtcg
     4441 agacggattt ccttgtcgaa atgcgcaccc tcgtccgagt gcaacgtgtc ccagaagcgc
     4501 cgagccgcat cccagtccgc gcctttcggc gcacgcggcc ggtccttgag gaaagcatag
     4561 gtcgcttcgt ccggcgcgat gagcccggcg cgggcgccgc cttcgatcgt catgttgcag
     4621 acggtcatgc ggccttccat cgtcagatcg cggatcgcct cgccggcaaa ttcgatcaca
     4681 tggccgttgc cgcccgcggt gcccatctcg ccgatgatcg cgaggatgat atccttggcg
     4741 aagacgcccg ggccacgcct gccgtccacg acgacacgca tgttcttggc cttcgactgg
     4801 atcagcgtct gcgtcgccag cacatgctcg acttccgacg tgccgatgcc atgcgccaaa
     4861 gcgccgaagg cgccatgcgt cgaggtgtgg ctgtcgccgc agacgattgt cgtgcccggc
     4921 agggtgaagc cctgctccgg cccgatgata tggacgatac cctggcggcg atccagctcg
     4981 tggaagtagt cgatgccgaa gtccttggca ttggcggcca gcgtctcgac ctggatgcgg
     5041 ctttcctcat ccttgatgcc ttccgagcga tccgaggtcg gcacgttatg atcgacgaca
     5101 gcgagcgtct tttccggtgc gcgcacgtcg cgcccggcaa ttcgcaaacc ttcaaatgct
     5161 tgcgggctcg tcacttcgtg gacgaggtgg cgatcgatat agagaaggca ggtgccgtcg
     5221 tcctgacgat cgaccacatg gtcatcgaag atattgtcgt atatcgtgcg taactcggcc
     5281 attggaaagc actcttcgtt gggttacgac ccttcgccgc ttgccggagc ggcgcgtctg
     5341 gcagaaactg aggcgttatt gacctcatcc cccatatagg aacagttata aaaacatcat
     5401 cgccactatg cggaggtgtc ttggctcgca aggcccgcag caccggagaa cagaatgcgt
     5461 ccgcccgtcg cgccctatct caccgtctcg ccggccatgg cggccatcgc tttttatacg
     5521 accgtcttcg gagccaagca gcaggcgctg atgccggcgc tcgatggctt acggatcatg
     5581 cattgcgaac tgacgatcaa cggcggctcg atcatgctgg ccgacgcatt tccggaattc
     5641 ggcaataccc gcgtgcctgt cccgggcgag ccgatgacgg cctcagtcag tctcgaattc
     5701 gagactgccg aagaagttga caccgtcttc aagcgcgcgg tcagcctcgg cagcaaaggc
     5761 gaaaccaatc cgacgaacag cttctgggga acgcggcttg caaccttccg cgaccccttc
     5821 ggccaccgct ggattcttaa cgccccgacg gcatagggcc ggcttagccc ttgccaccct
     5881 tcttcggcgc acggatggcg ccagtggcct tcttcaccgg cggcgccttt ttcacggtcg
     5941 gcgcctgggc attggccgcc tcacgcgcgg ccttctgcgc cggcgtctca agcttctcga
     6001 caatgcgggc cgacttgccg cgccgatcac gcaggtaata gagcttggcg cgccggacct
     6061 taccgcgacg cacgaccttg atcgaatcga tattcggcga atacaccggg aagacgcgct
     6121 cgacgccctc accataggaa atcttgcgaa cggtgaagct ctcgttgatc ccgccgccgg
     6181 tgcgggcgat gcagacgccc tcataagcct gaacgcgggt acgctcgcct tccttcacct
     6241 tcacgttgac aatcagcgta tcgccagcct gaaattccgg gaccggacgc actgcaacga
     6301 gcttggcgac ctgggccgct tccagctctt caataatatt agccatggta aaaccctgcc
     6361 gttccgcctg gttcggcctg ggccgcccgg cgagcgtttc ggaacgctgt tttaagttaa
     6421 gccgcggctt ctacacgaga ccgagagggt tgtcgagaca gacaacccca tttacgccgc
     6481 agcttgcctc aggcaaactt ttgcggcacg tgcacggcac gaatcaaaaa cgggcgggaa
     6541 ttcatcgaca tggccggcgc ctgcactgaa ggcctcgata ctggatttgt cgcgctgggc
     6601 tatgccaagg ttggttttct cggcatcatc cagggcatta cggagctttt gcccgtctcc
     6661 tcgacggcgc atatgcgcat tatccccgcc ctgctcgggt ggcgcgaccc cggctcggcc
     6721 ttttccgccg ccatgcagct tgctgcactt gccgccgtcg tcagctattt ctgggccgac
     6781 gtaaagggca tctccgaagg cgcggtcgca gcggtgaaac gtcgcgatta cgaggattgg
     6841 tcactccgtt tctgcgtgtg gatcgtgctc gcgacgctac cgatcatcat cgctggctcg
     6901 ttgctctcga aacttctgaa tacctgcaat tcgccgctcc gctcgctcgc cgcgatcggc
     6961 gtcgcctata tcgtcatggg ggcgctgctt gccgttgcgg aagtctattc gcgacacaaa
     7021 cgtacgctcg atcatgtcga tctgaaggac gcgatgatcg tcggtctcgc ccaatgcggt
     7081 gcgctgatcc caggcgtttc ccgctccggc tcgaccttca ctgcggcgct tttccgcgac
     7141 ctcgaacgca ccgaagcggc gcgcttttcc ttcctgctcg ggatcccggc cattgccgcc
     7201 gccgggatca aagaacttta cgaattgcat aaggcgcatc tcgacgccca cggctggtcg
     7261 atcctcggct ttggcctcgt cgtcgcgtca atttcggcgt ttatcgcgat ctggggcctc
     7321 ctgcgcattc tcgaacgctt tacgtcgtgg ccgttcgtta tctatcgggt cttactcggt
     7381 attttcctgc tcgccgcggt cgccgcccat tggttgccga attaagcggc agacgcggcc
     7441 acgggccgca atgtctggtg ccacggacgg gcccaacaaa tttttgccgc cctttacgtt
     7501 cccaaatcgc atcacggtga cgatgccagc cggtttgact aggccggcgc aaactggtct
     7561 acattcgcgt ccgtttgggg ttttacagag ccaatatgtc tgacgccgcc atcaaaaagc
     7621 cgggctccgg catcgctgga gaatttgctg aaag
//
LOCUS       DASWOG010000453         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_285547,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000453 DASWOG010000000
VERSION     DASWOG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285547"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1737
                     /locus_tag="VGB93_09410"
     CDS             286..1737
                     /locus_tag="VGB93_09410"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY0146349.1"
                     /translation="MNQIVRIASPANTSWLSRSLAISYFTVAALAGATDGVLITLSSV
                     FADWLYNGLIRHDIVQIDIALGIGFCGGLGFILVAKALGLYSMQVTLNPQKHVSKIVF
                     VWATVLLSLTAVLFLLRVGTIFSRGTIIIFAFTELGALLGARLVSAGLMRSAIAKGGI
                     VGRRAVIVGEPAELGHLSSKALLAHFGLKELARVTIAGAGEKTSEGIDVAALDLAIDV
                     ARRHHADELVIAVDWRRTDVIEKIKERFRASPLAVRLLPDRTVRATVGHQAEISLETM
                     LSVELQRAPLTPFERFVKRAFDTILATAAVIALSPLLLIVAIAIKLDSKGPVLFRQQR
                     TGFDGQKFPIYKFRSMSVMEDGPVVVQAARGDRRVTRVGALLRRSSVDELPQLFNVIK
                     GDMSLIGPRPHALAHDDHYGRLIATYAFRHHVKPGMSGWAQVNGQRGETAQVADMRKR
                     LEFDLWYINNWSLTLDLKIILRTCLEITRQQAY"
     gene            complement(1757..2317)
                     /locus_tag="VGB93_09415"
     CDS             complement(1757..2317)
                     /locus_tag="VGB93_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HEY0146350.1"
                     /translation="MAILDARKTKPLEGGASFSLGNRVYRALWQVSWAAFAAWTPPPL
                     FAWRTSLLRLFGADIAPSARVYGKTHVWYPPNLTMAENALLGPGVNCYNQARISIGAK
                     AVVSQGAHLCAGTHDINDPDFQLIAKPITIGAGAWVAAEAFVGPGVNIGDGAVLGARG
                     VAFRDIEPWAVYAGNPAKKLKDRPRF"
     gene            complement(2327..>2629)
                     /locus_tag="VGB93_09420"
     CDS             complement(2327..>2629)
                     /locus_tag="VGB93_09420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0146351.1"
                     /translation="ILPSLSEGLPMAILEAWAAATPALMSASCNLPEGFSAGAAFDTG
                     ITADCIAVALRQALALPEPDWQRMSAAAQSLARTRFSAKSVAAQWVEIYRGLAAAV"
BASE COUNT          473 a          840 c          786 g          530 t
ORIGIN      
        1 gtgccggcaa ggcagaatcc ggcaacggct gacggctcgg ggcggggctc cgtaaaattt
       61 tccgtatggt ttgatgacgg tgagcgaact ggccggttcg ctcaccgttt ttttgccatc
      121 gtttctgcgc ggaaatggcc caatcgttgc ttgagagaag ataaattata taggtgcggc
      181 gccgtacagg cgccgttcat acgaaaagag ttagacctct ccccacaaaa gggaaccttg
      241 atccctgcaa tttcattctc cgttctctgc gtcttgtgag cgttcatgaa tcagattgtg
      301 cgcattgcca gccccgcgaa cacctcgtgg ctttcccgca gccttgccat ttcctacttt
      361 accgttgcag ccctcgctgg cgccacggac ggtgtgctga tcacgctgtc gagcgttttc
      421 gccgactggc tgtataatgg gctgatccgc cacgatatcg tgcaaatcga tatcgcgctt
      481 ggcatcggtt tctgcggcgg ccttgggttc atcctggtag cgaaggcttt gggcctttac
      541 agcatgcagg tcacgctgaa cccgcagaaa cacgtttcca agatcgtctt tgtctgggcc
      601 accgttcttc tttcgctcac cgctgtgctg ttcctactgc gggtcggaac gatcttttcc
      661 cgcggcacga tcattatttt cgccttcact gaattggggg ctctcctcgg ggctcgcttg
      721 gtttcggcgg gactgatgcg ctccgccatc gcgaaaggcg gcatcgtcgg acggcgcgcg
      781 gtgattgtcg gcgaaccggc cgaacttggc catcttagct ccaaagcctt gttggcccat
      841 ttcggcctga aagagctggc acgcgtcacg atcgccggcg ctggcgagaa gacgtccgaa
      901 ggaattgatg tcgccgcgct cgatctggcg atcgacgtcg cccgccgcca tcacgccgat
      961 gagctcgtca tcgctgtgga ttggcgccgc accgacgtga tcgaaaagat caaggaacgc
     1021 ttccgtgcct cgccgcttgc cgtgcggctt ctgcccgacc gcaccgtccg cgcgacagtc
     1081 ggccatcagg cggaaatctc gctggagacg atgctttcgg tcgagctgca gcgtgcgccg
     1141 ctgactccat tcgagcgttt cgtcaaacgc gccttcgaca cgattctcgc gacggcggcc
     1201 gtcattgctc tctcaccgct gctgctcatc gttgcgatcg ccatcaagct cgacagcaaa
     1261 ggcccggtgc tgttccgtca gcagcggacg ggcttcgacg gccaaaaatt ccccatctac
     1321 aagttccgct cgatgagcgt aatggaggac gggccggtcg tcgtccaggc ggcccgcggt
     1381 gatcgtcgag tcacccgcgt cggcgcgctt ctgcgtcgct ccagtgtcga cgaactgccg
     1441 cagctcttca atgtcatcaa gggcgacatg tcgctcatcg ggccgcggcc acatgcgctg
     1501 gcgcatgacg atcattacgg ccgcctcatc gccacctatg ccttccgcca tcacgtcaag
     1561 ccgggcatga gcggttgggc gcaggtgaat ggccagcgcg gcgagaccgc gcaggtcgcg
     1621 gatatgcgca agcgcctcga attcgatctc tggtacatca acaattggtc gttgacgctg
     1681 gacctcaaga tcatcctgcg gacctgcctc gagatcaccc gccagcaagc ctattgaagc
     1741 tgagcgatcg ctgccgtcaa aagcgcggcc ggtctttcag cttcttcgcc ggattgcccg
     1801 catacaccgc ccagggctcg atatcgcgaa acgcgacgcc ccgggcgccg agcaccgcgc
     1861 catcgccgat gttgacgccg ggccccacga aggcttcagc cgccacccag gcgccggcgc
     1921 cgatggtgat cggcttggcg atcaactgga aatcaggatc gttgatatcg tgcgttccgg
     1981 cgcagagatg cgcgccctgc gaaacgacgg ccttcgcacc gatcgaaatc cgcgcctgat
     2041 tgtagcaatt gacgcccggg ccaagcagcg cgttttccgc catcgtcaag ttcggtggat
     2101 accagacgtg ggttttgcca tagacgcgcg cactcggcgc aatgtcagcg ccaaagagac
     2161 gcagaaggct cgtccgccag gcaaagagcg gcggcggtgt ccaggcggcg aacgcagccc
     2221 aactcacctg ccacaatgcg cgataaacac gattgccgag gctgaagctt gccccgcctt
     2281 ccagcggctt tgttttgcgc gcgtcgagaa tagccatggt tcacccttat accgccgccg
     2341 cgaggccgcg atagatctcg acccattgcg ctgcgaccga cttggccgag aaccgggtcc
     2401 gcgccagcga ttgcgccgcg gccgacattc tttgccagtc tggctcaggc agggccagcg
     2461 cttgacgcaa cgcgacggca atgcagtcgg cggtaatgcc cgtgtcgaat gccgcgccgg
     2521 cggaaaagcc ctcgggcagg ttgcaggacg ccgacatcaa tgcaggcgtg gcggcggccc
     2581 aggcttcgag gatcgccatc ggcaggcctt cgcttagcga cggcagaat
//
LOCUS       DASWOG010000454         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_340414,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000454 DASWOG010000000
VERSION     DASWOG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340414"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VGB93_09425"
     CDS             complement(<1..1067)
                     /locus_tag="VGB93_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0146352.1"
                     /translation="MTHSTVDSAITDLGLAAWGRKEIDIAETEMPGLMATRAEYGAAQ
                     PLAGARIAGSLHMTVQTAVLIETLSALGADIRWASCNIYSTQDHAAAAIAAAGIPVFA
                     RKGESLTDYWDYTHRIFEWGDGGAPNLLLDDGGDATLLIHLGVRAEGGDVAFLDKATN
                     EEEEILFAAIKRRLKENPGWYGRMGAAIKGVSEETTTGVHRLYITQKEGKLLWPAINV
                     NDSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRNAGCR
                     VIVSEVDPICALQAAMEGYEVATMEEAAPRADIFVTCTGNIDVIRLEHMRAMKDRAIV
                     CNIGHFDSEIQVASLRNFRWN"
     gene            1259..>1701
                     /locus_tag="VGB93_09430"
     CDS             1259..>1701
                     /locus_tag="VGB93_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0146353.1"
                     /translation="MALVTRELVSGPALGTAQIRPLLEKYGLRATRQRLGLAKLLFSK
                     GNRHITADVLASEAHEARMFASLATVYNVLNLFAEVGLVRSFTVEGGKTIFDTNTTDH
                     CHFYFEDSGRVSDISASTARFGDRFEAPEGSEVVKVDVVVRLRPIG"
BASE COUNT          317 a          485 c          538 g          361 t
ORIGIN      
        1 ttgttccacc tgaaattgcg tagcgaagcg acctgaatct ccgagtcgaa atggccgata
       61 ttgcagacga tggcgcgatc cttcatcgcc cgcatgtgct cgagacgtat gacgtcgata
      121 ttgccggtgc aggtcacgaa aatgtcggcc cgcggcgcgg cctcttccat ggtcgcgacc
      181 tcgtagcctt ccatcgccgc ctgcaaggcg cagatcggat cgacttcgga gacgatgaca
      241 cggcagccgg cgttccgcag cgaggcggcc gaacccttgc cgacatcgcc aaagcccgcg
      301 accatcgcga ccttgccggc catcatgacg tcggtgccgc ggcggatgcc gtcgacgagc
      361 gattcacggc agccgtacaa attgtcgaac ttcgacttgg tgacggaatc gttgacgttg
      421 atcgccggcc agaggagctt gccttccttc tgcgtgatgt agaggcgatg cacacctgtg
      481 gtcgtctctt cgctgacgcc cttgatggcg gcgcccatac gcccgtacca gccgggattt
      541 tccttcagcc ggcgcttaat cgcggcgaag aggatttctt cttcttcgtt ggttgccttg
      601 tcgagaaagg ccacgtcgcc gccttcggcg cgcacgccga ggtgaatgag cagggtcgca
      661 tcgccgccgt catcgagcaa aaggttcggc gcaccgccgt cgccccactc aaagatgcgg
      721 tgggtgtaat cccaataatc ggtgaggctt tcgcccttgc gtgcgaagac cgggatgccg
      781 gcggcggcaa tcgctgcggc ggcatgatcc tgcgtcgaat agatgttaca ggaggcccag
      841 cggatgtcgg cgccgagcgc gctcagcgtc tcgatcagaa ccgcggtctg gaccgtcatg
      901 tgcagggagc cggcaatgcg ggcgccggcg agaggctggg cggcgccata ttcggcccgc
      961 gttgccatca ggccgggcat ttccgtctcg gcgatatcga tctccttgcg gccccaggcg
     1021 gcaagcccca gatcggtgat ggcggaatcg acggttgaat gggtcatagg cgctccttga
     1081 aaactgcgcg gcggtctagc gcaaagccgg gccagacgca ataaagatat aaagaagtcc
     1141 ttatatgagc cgtccaaccc gtgtgttttt tgggattgtt cttaagtaag gctcgagggg
     1201 ttgatctgct ggcacggttt ttgttaaatt ctttgcttga taaggggtta tagggacaat
     1261 ggctttagtg acacgcgaac tggtttcggg accagcactc ggcacggcgc aaattcgccc
     1321 gttgctggag aaatacggtc tgcgtgccac gcggcaacga cttggcttgg ccaagctgtt
     1381 gtttagcaag ggcaatcgtc acattaccgc cgacgtcctg gcgtccgaag cgcatgaggc
     1441 gcggatgttc gcgtcgctgg cgacggtcta taacgtgctc aatctttttg ccgaagtcgg
     1501 cctcgtccgc agcttcaccg tcgaaggcgg caagacgatt tttgatacga atacgacgga
     1561 ccactgtcat ttttatttcg aggacagcgg tcgggtgtca gatatttccg caagcaccgc
     1621 ccgtttcggg gaccgtttcg aggccccgga gggctccgaa gtggtgaagg tcgatgtggt
     1681 tgtgcggctg cggccgatag g
//
LOCUS       DASWOG010000455         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_139452,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000455 DASWOG010000000
VERSION     DASWOG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139452"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1007
                     /locus_tag="VGB93_09435"
     CDS             99..1007
                     /locus_tag="VGB93_09435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0146354.1"
                     /translation="MLCRLARQSGAAALIFALATVSATAVGCGPDKLGTERTLTLSTN
                     GGFAIGLQTYPRTLALADHEVVLTFDDGPWPTTTPRVLDALAKECVKATFFLIGRNAQ
                     AYPGLARREIADGHSIGNHTFSHPARTLRLMRDAAARADIDHGFAADEQAAYGSVGDQ
                     IKFFRFPGFADTPALVSWLESRNIGMFGSDFWASDWLLLTPQAELDLVLKRLDRAKRG
                     ILLMHDSRASTAEMLPQLLRALKQRGYKIVHIAPGPDKPAFRPAPPGWRSETEEILAK
                     IIPRLAGHKAPGSHEAQTHTTRLPEP"
     gene            complement(1012..>1542)
                     /locus_tag="VGB93_09440"
     CDS             complement(1012..>1542)
                     /locus_tag="VGB93_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEY0146355.1"
                     /translation="TAFGGLFALVFAFAYGRVSGLGPRTVSALLALTGLICLYIVPNL
                     KYPSNPPSVGHPDTIGIRTGLYFAMMVLSVGAMVGAAILRKRLAPKHGAWTAALVASG
                     AYIVAVIVAAALLPPINEVPSDFPAVVLWHFRIDSLAMQAIMWATLGFVFGALTERDS
                     VGRLHRFSGNANPAWR"
BASE COUNT          314 a          502 c          465 g          261 t
ORIGIN      
        1 ctcttccagg ctcgtgatcg aagtcggtca tctgccaatc ctgactcgcg ctctatcgcc
       61 tggcggttca taaggagccc gtttcaggaa ggcaaacaat gctttgcaga ttagcgcggc
      121 aatctggtgc cgcggccttg atcttcgccc tcgccaccgt gagcgccacg gcggtcggct
      181 gcggaccgga taagcttggc acggagcgca cgctgacact ttcgaccaat ggcggcttcg
      241 ccatcggatt gcagacctat ccgcggacgc tcgcgctcgc cgaccacgaa gtcgttttga
      301 cgttcgacga cggcccctgg ccgacgacca cgccccgcgt gctcgatgcc ttggccaagg
      361 aatgcgtcaa agcaacgttc ttccttatcg gccggaatgc gcaagcctat ccgggcctcg
      421 cgcggcgcga gatcgctgac ggccattcga tcggcaatca cacgttttcg catccggccc
      481 ggacgctgcg gctgatgcgc gatgccgccg cccgcgccga tatcgaccac ggcttcgccg
      541 ccgacgaaca ggccgcttat ggcagcgtcg gcgaccagat caaattcttc cgctttccgg
      601 gcttcgccga tacgcctgcg cttgtgagct ggcttgagag ccgcaacatc ggcatgttcg
      661 gctccgattt ctgggcttcg gactggcttt tattgacacc acaagccgaa ctcgacctcg
      721 tgctgaaacg gctcgatcgc gccaagcgtg gcattttgct gatgcacgat tcccgcgctt
      781 cgacggcgga aatgctgccg cagcttttgc gcgccttgaa gcagcgcggc tacaagatcg
      841 tgcatatcgc gcccggtccc gacaagccgg cgtttcgccc ggcgccgccc ggctggcgat
      901 cggagaccga agagattttg gcgaagatca ttccaagact ggccgggcac aaggcgcccg
      961 gctcacacga agcgcagacg catacaacgc gcctgccgga gccttaaaag tctaacgcca
     1021 agcgggattg gcgttgccgc taaaccgatg caagcggccg acggaatccc gctcggtcaa
     1081 tgcaccgaac acgaagccca atgtcgccca catgatcgcc tgcatggcaa gtgaatcgat
     1141 gcggaagtgc cagagtacaa cggccgggaa atcgctgggg acttcgttga tcggcggcaa
     1201 gagcgcagcg gcgacaatca cagccacaat gtaagcgcca gatgcgacca gagcagccgt
     1261 ccaagccccg tgtttgggtg cgaggcgctt gcgcaggatc gccgcgccga ccatcgcgcc
     1321 gaccgacagg accatcatcg caaaataaag gccggtgcga atcccgatcg tgtccggatg
     1381 acccaccgag ggcggattgg acgggtattt gagattcggc acgatgtaga ggcagataag
     1441 gcccgtcagc gccagcaacg ccgacaccgt gcgcggaccg agaccgctga cgcggccata
     1501 agcgaaggcg aacaccaacg cgaacaggcc accgaaagcc gt
//
LOCUS       DASWOG010000456         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_322959,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000456 DASWOG010000000
VERSION     DASWOG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322959"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGB93_09445"
     CDS             <1..484
                     /locus_tag="VGB93_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0146356.1"
                     /translation="YAMTGWRIGYAAGPDKLIKAMDMLQGQQTSGACSIAQWASVEAL
                     NGPQDFIPERRKIFETRRDLVVSMLNQAKYLKCPSPEGAFYVYPSIAEAIGRKTPDGK
                     KISNDENFVASLLEAEGVAVVQGSAFGLGPNFRVSYAASTEALEDACTKIQRFCASLV
                     "
     gene            859..>2200
                     /locus_tag="VGB93_09450"
     CDS             859..>2200
                     /locus_tag="VGB93_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0146357.1"
                     /translation="MQTFVPKLRLIHRFAAVIITFAVLTAICASIFWHEFGVLRDLRL
                     DIIHRQYPARVAIVEAKASEANFTAFAYELAHADADTAQQTTWAFRDEAKRFRNWIAI
                     AHAFLPESQDDLDGISTRFDKMLAFLETFAHRPAGGEAPEFQIDYRSGPLRDDLEASL
                     NHVSNEIAGNMNDRIVHVDNVTNPHFQRLSAFFAAGFIICFLGALIWASLAVARPILR
                     LATAMRVIASGQLDAEIGYTRGSDEVGDMARAIEVLRDKGRALRTLEAETRSAEARAH
                     QALQAEREHFVDVFQDDVLQVIDALSTATAELQRYAAAMRDIAHSTDDRTKHVVRSSR
                     VSVEMVEALGFASQEFSTLVDAANQDFFNANQIAARAANDGQVTSTSARDLAQAVETI
                     GQIADFIGGVAYQTNLLALNATIEAARCGEAGRGFAVVAGEVKALAQETSRAAAD"
BASE COUNT          417 a          690 c          640 g          453 t
ORIGIN      
        1 ctatgccatg accggctggc gtatcggcta tgccgcaggc ccggacaagc tcatcaaggc
       61 gatggacatg ctgcaaggcc agcagacgtc cggcgcctgt tcgatcgcgc aatgggcgtc
      121 ggttgaagcc ctcaacgggc cgcaggattt catccctgaa cggcggaaga ttttcgagac
      181 gcggcgcgat ctcgtggtct cgatgttgaa ccaggcaaaa tatctgaaat gcccgtcgcc
      241 agagggcgcg ttctacgtct atccgtcgat tgccgaagcg attggccgca agacgccgga
      301 cggcaagaaa atctccaatg acgaaaattt cgtcgcgtcg cttttagagg cggagggcgt
      361 cgctgtggtg cagggctcgg cctttggtct cggcccgaac ttccgcgtgt cttatgccgc
      421 ctcgaccgag gcgctcgaag acgcctgcac caagattcag cggttctgcg caagtcttgt
      481 ctgattttcg cgtcgtctga ggttcagtcg tcgcgacccg atatgacgga acggcccagg
      541 tcaaactggg ccgttttgtt ttgcccaatc cttcttgggc tccagacgcg aaaaagcccg
      601 gttcccccct cggaaccggg cttttgcccc ccgcgccctg tgtctgtgcc gtcgcccccg
      661 accggcagaa caggcagcca gtgaattcga tatagcaaag cgacccaccg aagtctgttg
      721 caattggaca acaatgggat tgcaaagccg cggccgcggg gcccttccga aacgctgtca
      781 caccagagca acacgtttcg ctaacccctt taagcttagg gtgcaccgcg catccgattc
      841 gccatccagc agattcaagt gcaaacattc gttccaaagt tgcggctcat ccaccggttt
      901 gccgcggtca tcattacgtt cgccgtcttg acggcgatct gcgcttccat cttctggcat
      961 gaattcggtg tcctgcggga tctccgcctc gacatcattc atcgccaata tccggcccgc
     1021 gtcgcaatcg tcgaagccaa ggcttcggaa gcaaacttca ccgctttcgc ttacgaactc
     1081 gcgcatgctg atgcggacac cgcgcagcag acgacatggg cgttccgtga tgaagcgaag
     1141 cgtttccgca attggattgc gattgcccat gccttcctgc cggaatcgca ggatgacctc
     1201 gacggcatat ccacgcgctt cgacaagatg cttgcgtttc tcgaaacgtt cgcgcatcgg
     1261 cctgcgggcg gcgaggcgcc ggagtttcag atcgattatc gctccggtcc attgcgcgat
     1321 gatctcgaag cgtcgctcaa tcacgtctca aatgaaatcg ccggtaacat gaacgaccgc
     1381 attgtccatg tcgacaatgt gaccaatccg cattttcagc gcctgagcgc gtttttcgcg
     1441 gcgggcttca tcatctgctt tttgggcgcg ctgatctggg cctcactggc tgtcgcgcgg
     1501 ccaattttgc ggcttgccac cgccatgcgc gtgatcgcgt ccggacagct cgatgccgag
     1561 atcggttata cgcgcggctc ggacgaagtc ggtgatatgg cacgcgccat cgaggttctt
     1621 cgcgacaaag gccgtgcgtt gaggacgctg gaggcggaga cccgctcagc cgaagcccgc
     1681 gcccaccagg cgctgcaagc ggagcgcgaa catttcgtcg atgtcttcca agacgacgtc
     1741 ttgcaggtta tcgacgcctt gtcgacggcg acggccgaac tccagcgcta cgcagcggcg
     1801 atgcgcgaca tcgctcattc gaccgacgat cgcacgaagc atgtcgttcg ctcgtctcgt
     1861 gtcagcgtcg aaatggtgga agctctcggc ttcgcgtcgc aggaattcag caccctcgtc
     1921 gatgctgcca atcaggattt tttcaacgcc aatcagattg ccgcgagggc ggccaacgat
     1981 ggccaggtca cgtccacctc ggcgcgcgat ctcgcccagg ccgtcgaaac cattggccag
     2041 atcgccgatt tcatcggcgg cgtcgcctat caaaccaatt tgctcgcgct caacgccacg
     2101 attgaggcgg cgcgctgcgg cgaggccggg cgcggttttg ccgtcgtcgc cggcgaggtc
     2161 aaagccttgg cgcaagagac gtcgcgcgcc gcggccgaca
//
LOCUS       DASWOG010000457         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_249407,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000457 DASWOG010000000
VERSION     DASWOG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249407"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..704)
                     /locus_tag="VGB93_09455"
                     /pseudo
     CDS             complement(3..704)
                     /locus_tag="VGB93_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
     gene            complement(707..1582)
                     /locus_tag="VGB93_09460"
     CDS             complement(707..1582)
                     /locus_tag="VGB93_09460"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0146358.1"
                     /translation="MNLFCFGLGYSAQHFIKAYGEHFETIAGTARSYYAIERLGDPRI
                     KGFMFDAERADPEIATALAQADVLLVSIPPDTSVEPTLAKFGRVLSQLPQQVKIIYLS
                     TIGVYGDRQGEWVDETRVPVPKQPRSTSRLRAEKAWQALSRDARKTIQILRLAGIYGP
                     GRNALVQLKNGTAQRVIKPGQVFNRIHVTDISTAIAAALAYEGDSDVWNVADDEPAPP
                     QDVITYAAKLMGIEPPPEEDFETAPLSAMARGFYEENKRSANGKMKEGLGVTLAYPTY
                     RDGLNALWDAGEGRR"
     gene            complement(1579..2417)
                     /gene="queG"
                     /locus_tag="VGB93_09465"
                     /pseudo
     CDS             complement(1579..2417)
                     /gene="queG"
                     /locus_tag="VGB93_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205353.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
BASE COUNT          464 a          713 c          734 g          506 t
ORIGIN      
        1 ggctaagcgg ttttggccgc cgtctggccg aagagatgtt tgcgtgcttc ctcatccggg
       61 acggacggcg gcgccgcgtt gatctctttc gccttggcat aggcggcgat tgtgcccggg
      121 cgcgctttaa tcgtctcgaa ccagcgtttc agatgcggaa aatcatcgag gttctggcct
      181 tgcgccgcat gcggtacgat ccagggataa gaggccatgt cggcgatcga ataatcgtcc
      241 gcgacatact catgatccgc gagccgcttg tttagtacgc catagagccg gttggtctca
      301 ttcgtatagc ggtcgatcgc gtaagggatc ttttccggtg cgtaattgcg gaaatgatga
      361 gtctggcccg ccatcgggcc gaggccgccc atctgccaga acagccattc aatagtcgcg
      421 acccgcttgc gaacgtcgga cgaaataaat ttttcagtct ttgttgcgag ataaaacagg
      481 atcgcgccag attcgaaaac ggagatcggc tcgccgctgt cttttggcgc acggtcaatt
      541 atggaaggga tgcgattgtt gggtgcgatc gccagaaact ccggcttgaa ctgttcgccg
      601 cggccgatgt tgacggcgtg gatcgtatac ggaacctgtg cttactcaag aaacatcgta
      661 atcttgtggc cgttcggcgt tgtccagtaa taaaggtcga tcattcttat ctccgtcctt
      721 cgccggcatc ccacagcgcg ttcaatccgt cgcgataagt tggataggcc agcgtaacgc
      781 cgagcccttc cttcatcttg ccgtttgccg agcgcttatt ctcctcatag aagccgcgcg
      841 ccatcgcact caagggggcc gtctcgaaat cctcttccgg cggcggctcg atgcccatca
      901 atttcgcggc ataagtgatg acgtcctgag gcggcgccgg ctcatcgtcc gccacgttcc
      961 agacatcgct gtcgccttca taggcgagcg cggcggcgat agccgtgctg atgtcggtga
     1021 cgtgaatgcg attaaacacc tgacccggct tgatcacgcg ctgcgccgtg ccattcttca
     1081 attgcacgag cgcgttgcgg ccgggaccat aaatcccggc gagccgtaaa atttggattg
     1141 tttttcgcgc gtcgcggctc agcgcctgcc aagccttttc cgcccgcagg cgtgaggtcg
     1201 agcgcggctg tttcggcacc ggcacccgcg tttcgtcgac ccattcgccc tgccggtcgc
     1261 catagacgcc gatggtcgag agatagatga ttttgacctg ctgaggcagt tgcgacagca
     1321 cgcggccgaa tttggcgagc gtcggctcaa ccgatgtgtc gggcggaatc gagacaagca
     1381 gcacgtcggc ctgcgcgagg gcggtggcaa tctccgggtc ggcacgctcg gcatcgaaca
     1441 tgaagccttt gatgcgcgga tcgcccaagc gctcgatggc atagtagctg cgcgccgtgc
     1501 cagcgatcgt ttcgaaatgc tcgccatagg ctttgatgaa atgctgcgcg ctgtagccga
     1561 ggccgaagca gaaaaggttc attgtatcat ctcgattggt gcatcggccc gtgcccattc
     1621 cgctagaaca tcagggtcgt cttctgtttg tcgccgcata cgcaattctg caaaccgcca
     1681 cggtgccacg cggccgatgg cccagaccgc catcgcgcgg acgagcggcg aggcatcgtc
     1741 gatcagcgcc tcgatgtgcg gctgcaaagc atcatcgctg gaattgcctg ccgcgatgag
     1801 aacattgcgc agaaagcgcg cacggccgat gcgcttcacc gggctgcccg aaaagagcgc
     1861 gcgaaaacct gcatcatcca atcgcaataa atctccaatt ttcggcgcga cgagatcggc
     1921 gcgcgcctgc aatttcgcct cgcgcgaggc ttgcgcgaat ttgttccacg gacaggcggc
     1981 gaggcaatca tcgcagccat agatgcgatt gccgatggct tcgcgaaatt cgacgggaat
     2041 aggccccttg ttctcgatcg tcagatgcga gatgcagcgt cgcgcatcga ggagataggg
     2101 ttgcggaaac gcattggtcg ggcaggcatc gagacaggcg cggcattggc cgcaatgatc
     2161 cgtctcggct tcgtccggtg acaggtccag attcgtgaac agcgcgccga gaaagagcca
     2221 ggagcctgcc tcgcgcgaca cgaggttcgt atgcttgccc tgccagccga ggcccgcggc
     2281 ctgcgccatc ggcttttcca tcaccggcgc ggtatcgacg aagactttga tctcgctgtc
     2341 gggcgcctgc gccgcgggcc acgaaccaag aagcttaagc cgcccctaga tcaggtagtg
     2401 atagtcgcga tggcgcg
//
LOCUS       DASWOG010000458         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_323018,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000458 DASWOG010000000
VERSION     DASWOG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323018"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1326)
                     /locus_tag="VGB93_09470"
     CDS             complement(85..1326)
                     /locus_tag="VGB93_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0146359.1"
                     /translation="MLAAGFSLVCASAFAQPSEGSLGASVEGLLAAGRRLSPALRAAS
                     LETMAAGAKAAGADKLDDPTISDGYQFYRDPGVFSAHTVMLSQSIPLWGKLDLRRDAA
                     IAELEAARGKERAAEDALDENIKVAYAIYYLTAREIEINQHIGELDVQMHAAASARYN
                     EGGGDQSAIIQASDEETTAQTEIIKLQGDEGAARARLNALVGRPADAPLAEPQRLRRV
                     PAQMPALQILLDRSLSSNPTLSASQSEISAAKARKELADKAWYPDITLGSGPIVQTNR
                     EPVGFAATVGLNIPVPWGRESSDQAQATAQLGASEAQYDAATHEVEGALGEALAKLRA
                     SRKIDFLLQRESTPQARAMFQTLLANYSQGKGDLASVIAAEHQLHEVDLRHLQALLDE
                     QTSFAAVERLVGGEICCVACL"
     gene            complement(1364..1762)
                     /locus_tag="VGB93_09475"
     CDS             complement(1364..1762)
                     /locus_tag="VGB93_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY0146360.1"
                     /translation="MTRTFILAGATAFVFGMGFSMPPSFAAEADYRFELVGQPQPAAG
                     KDIVRVRLVRASDGKPVTDAVIFESTADMGPSGMATMPAPVKPLPPKDGVYRFEVAPG
                     MTGTWALHLAAKVQGETGTVKGTINANLVN"
     gene            complement(1861..2259)
                     /locus_tag="VGB93_09480"
     CDS             complement(1861..2259)
                     /locus_tag="VGB93_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146361.1"
                     /translation="MSVSDETASFVVLMLRILRHILLVLVAITLVGGAAAESARAASH
                     GPMMLMNGIPCDMQMPASDLDHSKPMAPCKGMTHDCLNMAGCATINALPAQFVLQEFP
                     AFQRVRAVWADVFDRASLYHQPELLPPRTA"
     gene            complement(2316..>2695)
                     /locus_tag="VGB93_09485"
     CDS             complement(2316..>2695)
                     /locus_tag="VGB93_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY0146362.1"
                     /translation="LRGEGRVVAMAGDGVNDAPALAEADVGVAMGTGTDVAMQSAGVT
                     LVKGDLAGIARARALSQATMRNIRQNLALAFVYNLLGIPLAAGVLYPAFGLLLSPVIA
                     AAAMSLSSVSVISNALRLRLTRL"
BASE COUNT          560 a          830 c          807 g          498 t
ORIGIN      
        1 cgggagcctg atagaagagc ggctgacggg cgagggcctc ggccgcggca aatcccgagg
       61 aaagaacgcc ggccagcgca agagtcagag acaagcgacg cagcatattt cccccccgac
      121 caggcgttcg accgcggcaa acgaagtctg ctcgtcaagc agcgcctgga ggtggcgcaa
      181 gtcgacctca tgcaattgat gttcggcggc gatgaccgag gcgagatcgc ccttgccctg
      241 gctgtaattt gccaaaagcg tttgaaacat tgcgcgcgct tgcggcgtcg actcgcgttg
      301 cagcaagaag tcgatttttc gggacgcgcg cagcttggcg agagcctccc ctagcgcgcc
      361 ttccacctcg tgtgtggcgg cgtcgtattg cgcctccgac gcccctaatt gtgcggtggc
      421 ctgggcctgg tccgacgatt cgcggcccca tgggaccgga atattgagcc ctacggtcgc
      481 cgcaaatccc accggctcgc gattggtctg tacgatcggg ccgctgccaa gcgtgatgtc
      541 gggataccag gctttgtccg ccaattcctt ccgcgccttg gcggccgaga tctcggactg
      601 actcgctgac agcgtcggat tactcgaaag cgaacgatcc aaaagaattt gaagcgccgg
      661 catttgcgcg ggaacccgtc tcagccgttg cggctccgca agcggcgcat cggcaggacg
      721 accgaccaaa gcattcagcc tggcgcgcgc ggcgccctca tccccctgca atttgatgat
      781 ttcggtttgc gccgtcgtct cttcatccga agcttggata attgcggact gatcgccgcc
      841 gccctcgttg tagcgcgcgg acgcggccgc gtgcatttga acgtctaatt ctccgatgtg
      901 ctgattgatc tcaatttcgc gcgccgtcag ataatagatc gcgtaggcga ccttgatatt
      961 ctcgtcgagc gcgtcttcgg ccgcccgttc tttcccgcgc gcggcttcga gttcggcgat
     1021 cgccgcgtca cgccgcaaat ccaatttgcc ccagagagga atcgactggg acaacatgac
     1081 agtatgtgcg ctgaagacgc cgggatcgcg ataaaattgg tagccgtcgc tgatggttgg
     1141 atcatcgagc ttgtcggcac cggctgcttt ggcgccagcc gccatagtct caagcgaagc
     1201 ggcgcgcagc gccgggctca ggcgtcgccc cgcggcgagc aatccttcga cggaagcgcc
     1261 cagcgagccc tcggaaggct gagcgaacgc cgacgcacat accaagctga agccggccgc
     1321 caacatcgcg ctggcgccaa accacgtccg ctgggcgtac acgtcagttc accagatttg
     1381 cgttgatggt tcctttgacg gttcccgtct cgccttgaac cttggcagcc agatgcaagg
     1441 cccatgtgcc ggtcatgccg ggggcgactt caaagcgata gacgccatct ttgggtggaa
     1501 ggggtttaac gggcgccggc atggtcgcca tgccggacgg gcccatatcg gccgtgcttt
     1561 caaagatcac ggcgtctgtt acgggcttgc catcactggc gcgaacaagc cgcactcgaa
     1621 cgatgtcttt gccagccgcg ggttggggct gcccgaccaa ttcgaaccga tagtcagcct
     1681 ctgcggcgaa actgggcggc atggagaatc ccatgccgaa gacgaacgcc gtagcgcccg
     1741 caagaatgaa agtgcgagtc acggaacacc tcttcatagc cggccggcgg cttaagcgcg
     1801 cggcaagcag aacaaccaga ttgattcatt gagaaatgcg cacgcgtgtg cgcgaaccaa
     1861 tcaggcagtt cgaggaggca aaagttccgg ttgatggtag aggctggccc gatcgaacac
     1921 atcggcccaa acagctctca cacgctggaa ggcaggaaat tcctgcagga cgaactgagc
     1981 tggtaaagca ttgatcgttg cacagcccgc catgtttaga cagtcatgcg tcatgccctt
     2041 gcaaggcgcc attggtttcg aatgatccaa atccgaagcc ggcatttgca tatcgcaagg
     2101 gataccattc atgagcatca tcggcccatg tgacgcggca cgagctgatt ctgccgccgc
     2161 ccccccgacg agggtgatcg cgacaagaac aagcaggata tggcgcaaga tacgaagcat
     2221 gagcacaaca aagctagcag tttcgtccga tacgctcaag cccacgcgac cgcgggacgc
     2281 tgacgcatat tatcgtgaat ccaggttctt ttttctcaca aacgcgtcaa ccgcagccgc
     2341 aaggcgttac tgatcaccga caccgaactc aggctcatcg ctgcagccgc tatgacggga
     2401 ctgaggagca gaccaaaggc cgggtaaagc acgccggcgg caagcgggat gcccagcaga
     2461 ttgtagacga acgccaatgc aaggttctgt cggatattac gcatcgtcgc ctggctgagc
     2521 gcgcgggcgc gtgcaattcc ggcaagatca cctttgacca aggtaacgcc ggcgctttgc
     2581 atggcgacat cggttcctgt gcccattgcg acgccaacat ccgcttcagc gagggccgga
     2641 gcgtcgttga cgccgtcacc ggccatggcc acaacgcggc cttcacctct cagcc
//
LOCUS       DASWOG010000459         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_231395,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000459 DASWOG010000000
VERSION     DASWOG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231395"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGB93_09490"
     CDS             complement(<1..1005)
                     /locus_tag="VGB93_09490"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF044583.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0146363.1"
                     /translation="MRDYQDVGGLKIARPLYDFVTEEALPGTGITAASFWDGFEKLID
                     EFAPQIQAHMQLRDALQSKIDDYHVSHRGLPFDPVDYEKFLREIGYLVPEPPDFSIAT
                     ENVDDEIALVPGPQLVVPVSNARYVLNAANARWGSLYEALYGTDAISSADGGERGKGY
                     NKIRGARVVTWGKEFLDASVPFVGASYLDVAGLRVGNGKLIATLADGSETGLKNPAQL
                     RAYRGQADAPTAVLLRNNGLHIELVVDRASRIGGDDPAGVADILIEAALTAIMDLEDS
                     VAAVDAADKVGLYRNVLGLMQGTFSANVEKNGKIIERRLADDCTYTQLDGAPLSVSGR
                     S"
     gene            complement(998..>2257)
                     /locus_tag="VGB93_09495"
     CDS             complement(998..>2257)
                     /locus_tag="VGB93_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY0146364.1"
                     /translation="VAASEHRIRVRLVKTTAWHALFARNMFILPPANEFDAFVPDYVI
                     LHAPGLSVEPAALGLRSPTAIALSFAQKLIVISGTQYAGEVKKAIFTVMNWLLPAKGV
                     LPMHCSANIGPDGDSALFFGLSGTGKTTLSSDPERWLIGDDEHAWSPAGVFNFEGGCY
                     AKVINLRQEAEPEIWAAAHRFGTVLENVVLDANGHVDLDDDSLTPNTRACYPLHFIAN
                     AEPSGAGGVPRNVVMLTADAFGVVPPLSRLTPEQAMYHFLSGYTARVAGTEKGLGKEP
                     VATFSACFGAPFMPRPPEVYGRLLMHYLSKQKTNVWLVHTGWTGGPYGIGTRISIAHT
                     RALLRAALTGALDRAPFREEPYFGLNVPLHVAGVPEDMLDPSRAWPDPQAYAAQARHL
                     VHLFEENFARFAGTAAELDMQEKAVHA"
BASE COUNT          476 a          690 c          679 g          412 t
ORIGIN      
        1 gctgcggccg gaaaccgaga gcggcgcgcc gtcgagttgc gtgtaggtac aatcgtcggc
       61 aaggcggcgc tcgatgattt tgccgttctt ttccacattg gcggaaaacg ttccctgcat
      121 caggccgagc acattgcgat agagcccgac cttatcggca gcgtcgacgg ccgcgacact
      181 atcctcgagg tccatgatcg ccgtcagtgc cgcctcgatg agaatatctg cgacgccggc
      241 gggatcgtcg ccgccgatcc ggcttgcacg atcaacgacg agttcaatat gcagcccgtt
      301 gttgcgcaag aggaccgccg tcggcgcgtc ggcttggccg cgataggcgc gcaattgcgc
      361 cgggtttttc agtccggtct cggaaccatc ggcgagcgtt gcgatgagtt tgccattgcc
      421 gacgcgaagg cctgccacat cgagatagct cgcgccgacg aacggcaccg aagcatcgag
      481 aaactctttg ccccaggtga caacacgagc gccccggatt ttgttgtagc ccttgccgcg
      541 ttcgccgcca tccgctgaag aaatcgcatc cgtgccgtaa agggcctcgt aaagcgatcc
      601 ccaacgcgca ttggccgcat tcaaaacgta gcgcgcattc gaaaccggca cgaccaattg
      661 cggacccggc acaagcgcga tctcatcgtc gacattttcg gtcgcgatgg aaaaatccgg
      721 tggctcgggc acgagatagc cgatctcgcg cagaaatttt tcgtaatcga ctggatcgaa
      781 cggcaggccg cgatggctga catgataatc gtcgatcttc gactgcaacg catcgcgaag
      841 ttgcatgtgc gcctgaatct gcggcgcaaa ttcgtcaatg agtttttcaa acccgtccca
      901 aaacgacgcc gctgtaatgc cggtgcccgg taacgcttct tcggtgacga aatcataaag
      961 cgggcgagcg atcttaagac cgccaacgtc ttgatagtca cgcatggacg gctttctcct
     1021 gcatatcaag ttccgccgcc gtgccggcga atctggcgaa attctcttca aacaggtgga
     1081 cgaggtgacg tgcctgcgcc gcataggctt gcggatccgg ccacgcgcgc gagggatcga
     1141 gcatgtcttc cggcacgccc gcgacatgca gcggcacgtt caggccaaaa tagggctcct
     1201 cgcgaaatgg cgccctatcg agtgcgcccg tcagcgccgc gcgcagcagg gcccgcgtat
     1261 gggcgatcga aatgcgcgtg ccgattccat aaggaccgcc cgtccagccg gtgtggacga
     1321 gccagacatt ggtcttttgc ttcgacagat agtgcatcag aaggcggcca tagacctccg
     1381 gcgggcgcgg catgaacggc gcgccgaaac aggcgctgaa ggttgcgacc ggctccttgc
     1441 ccagcccctt ctccgtcccg gcgacgcgcg ccgtgtagcc agagaggaaa tgatacatcg
     1501 cctgctcggg cgtgaggcgc gacaatggcg gcacgacgcc aaaagcatcc gctgtgagca
     1561 tcacgacatt gcgcggaaca ccgcccgcgc cgctcggctc ggcattggca atgaagtgga
     1621 gcggatagca ggcgcgcgtg ttgggcgtca gcgaatcgtc gtcgaggtcg acatggccgt
     1681 tggcatcaag gacaacattt tccagcaccg tcccgaaacg atgtgccgct gcccaaattt
     1741 caggctcggc ctcctgtcgc agattgatga ccttggcata gcagccgccc tcgaaattga
     1801 agacgccagc tggcgaccac gcgtgttcgt cgtcgccgat gagccaacgc tccggatccg
     1861 atgacagcgt tgttttgcct gtgccggaaa ggccgaaaaa aagtgcacta tcgccatccg
     1921 gaccgatatt cgcagaacaa tgcatcggca acacaccttt cgcgggcagc agccaattca
     1981 taacggtgaa gatcgccttc ttcacttccc ctgcatattg agtgccggaa atgacgatca
     2041 gcttttgcgc aaacgaaagc gcgatcgcgg tcggcgaacg cagcccaagg gccgccggct
     2101 ccacggaaag cccgggcgcg tgcaaaatga catagtccgg cacgaaggcg tcgaattcgt
     2161 ttgcgggcgg caggatgaac atgttgcggg caaaaagcgc gtgccaggca gtggtcttta
     2221 ccagccggac cctgatgcga tgctcggacg cggcaac
//
LOCUS       DASWOG010000460         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_268122,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000460 DASWOG010000000
VERSION     DASWOG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268122"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGB93_09500"
     CDS             complement(<1..300)
                     /locus_tag="VGB93_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0146365.1"
                     /translation="MATDFGADIAAVQGISEVPRILEVVCRSSGMGFAAVARVTEQRW
                     ICCASRDEIEFGLAPGGELAVETTICREIRKSHEAVVIDHVAEDAAFCGHHTPAQY"
     gene            complement(421..627)
                     /locus_tag="VGB93_09505"
     CDS             complement(421..627)
                     /locus_tag="VGB93_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0146366.1"
                     /translation="MTDEDERELEAELARLQQEHRDLDAAIDALHQSPAPDLLRLQRL
                     KKRKLQLRDRIAFIEDQITPDIIA"
     gene            913..1098
                     /locus_tag="VGB93_09510"
     CDS             913..1098
                     /locus_tag="VGB93_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0146367.1"
                     /translation="MAIQAHLVELERKHKVLENELHEALVHLSTDDLQIVELKRRKLM
                     VKDEIERLRHTASETIH"
     gene            complement(1267..2370)
                     /locus_tag="VGB93_09515"
     CDS             complement(1267..2370)
                     /locus_tag="VGB93_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0146368.1"
                     /translation="MDKVDCVVIGAGVIGLAIARRLAKAGREVIVLEAAEGIGTITSS
                     RNSEVIHAGIYYKAGSVMARMCVDGKRALYRYCAERGIPHRNCGKLIVATTPNETTKL
                     QSIRAHAEANGVTDLQTLDGDAARALEPALNCDAALLSPSTGIIDSHAYMLALRGEAE
                     DAGAAFAFHTPLLAARARDGRIEVDAGGDAPMSLECRLLINAAGLGATAVARSIDGMP
                     IDLIPPAYLAKGNYFSCSARAPFSHLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWVD
                     AIDYAVDPARAERFYPAIRRYWPTLPDVALMPSYSGMRPKIVPPAVAVQDFLIQGPRD
                     HGIGGLINLFGIESPGLTSSLAIADYVGELADA"
BASE COUNT          468 a          753 c          734 g          461 t
ORIGIN      
        1 gtattgcgcg ggcgtgtgat gcccgcaaaa ggcggcatct tcggccacat ggtcgatcac
       61 gacagcttcg tggctcttgc ggatctcgcg gcaaatggtg gtttcgaccg ccaactcccc
      121 gcccggcgcc aggccgaact cgatttcgtc gcgcgaagcg cagcaaatcc atcgctgctc
      181 ggtgacgcgc gccaccgcgg cgaaacccat gccgctcgac cgacagacaa cttcaagaat
      241 gcgcgggact tccgagatac cctgcacggc agcgatatcc gcgccgaagt cggtcgccat
      301 aatccgcttt ctgtgagtgt cccggcgaaa cgctagcgca gctgccgcga cgagtcgaga
      361 aatccgtcat gcaggaaccg caacggcgcc aaagccaagc aatccaaacc aacgccaaat
      421 ctacgcaata atgtccggcg tgatctggtc ttcgatgaag gcgatgcggt cgcgcaattg
      481 cagcttgcgt ttcttcagcc gctgcagccg cagcaaatcc ggcgccgggg attgatgcag
      541 cgcatcgatc gcggcgtcga gatcgcggtg ctcctgttgc agccgtgcaa gctcggcttc
      601 gagctcgcgc tcatcttcgt cggtcatgat ttatgttagc cggaaaacgc ggatgagatg
      661 gacaggttgc ggaagccgca gccaagaatg aagctaggaa tatcgtccct gccgggccgg
      721 tccgcaagct ggagcggatg gccggccgga gcgatcttcg ggttcgatac atagcacggt
      781 tccatcctca cgattcgtta cagatacagc cgaaatatga ggacggcatt ttcaataccc
      841 atcgacagat tcggccactt gtgtacactc atttgtccgg actgaggtct catccaacgg
      901 aggaggtttc gtatggcaat tcaggcgcat ctcgttgaac tggaacgcaa acacaaggtt
      961 cttgaaaacg aactgcacga agctctcgtg cacctgtcaa cggacgatct gcaaattgtc
     1021 gagttaaagc gccggaaatt gatggtaaaa gacgagatcg agcggttgag acataccgcg
     1081 agcgaaacaa tccattagca aatagtacaa gccggcgagc gtcgtgcttt gccaccgggg
     1141 tcggcgcatt gcaccacccg acgatggttt gcagccgttc gtttgcgttg cgctttgccg
     1201 ggcggcttgc tgctttcctt gccgctttcc ggcaagtcgc tcaaggcaag tcgcgcttgc
     1261 tgacgttcat gcgtccgcga gttcgccgac ataatcggcg atcgccaatg acgatgtcag
     1321 acccggcgat tctattccga acagattgat caggccgccg atgccgtgat cgcgcggccc
     1381 ctggatcagg aaatcctgca ccgcaaccgc cggcggcacg atcttcggcc gcatgcccga
     1441 atagctcggc atcagtgcga cgtctggcag cgtcggccag tagcggcgga tcgccgggta
     1501 aaatctttcc gcccgcgccg gatcgacggc gtagtcgatc gcatcgaccc attccacgtc
     1561 cggaccgaac cgcgcctgtc ctgccatgtc gagggtgagg tgcacgccga ggccgccagg
     1621 ctcgggcacc gggtagatca ggtgcgagaa cggcgcccgc gcgctgcaac tgaaatagtt
     1681 gcccttggcc agataggccg gcgggatcag gtcgatcggc atgccgtcga tgctgcgcgc
     1741 aaccgccgtg gcgccgagcc ccgcggcgtt gatcaaaagc cggcattcca gcgacatcgg
     1801 cgcatcgccg ccggcatcga cctcgatccg gccgtcgcgt gccctcgccg ccaataacgg
     1861 ggtgtgaaaa gcaaaggccg cgcccgcgtc ttcggcttcg ccgcgcaacg ccagcatata
     1921 ggcgtggctg tcgatgatgc cggtggaggg ggagagcagg gcggcgtcgc agttcaacgc
     1981 cggctccagc gcgcgggccg catcgccgtc gagtgtttgc agatcggtca cgccgttggc
     2041 ttcggcatgc gccctgatcg attgcagctt tgtggtctcg ttcggcgtcg ttgcgacgat
     2101 cagcttgccg caattgcgat gcggaatgcc gcgctccgcg caatatctgt agagcgcgcg
     2161 cttgccgtcg acgcacatcc gcgccatcac gctgcccgcc ttgtaataga tgccggcgtg
     2221 gatcacctcg ctgttgcgtg acgaggtaat cgtgccgatg ccttcagccg cctccaggac
     2281 gatgacctcg cggccggcct tcgccagccg ccgcgcgatc gcgagaccga tcacgcccgc
     2341 accgatgaca acacagtcga ctttatccat tcgccgtttg gatccggatt gttggttgtt
     2401 tggcaacgct tgcgct
//
LOCUS       DASWOG010000461         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_305063,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000461 DASWOG010000000
VERSION     DASWOG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305063"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1981
                     /locus_tag="VGB93_09520"
     CDS             356..1981
                     /locus_tag="VGB93_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0146369.1"
                     /translation="MSESVYSRDALSERLDFLGLDERMRTTLRDLTPVLTQAIRPALN
                     TFYDKVRATPHMRAFFSDDRHIAAAQSHQEKHWALIAEANFDTDYTNAVRRIGQAHAR
                     IGLEPRWYIGGYAAVLTELVRTLVKEQWPRLTQLSKRSPQSVAESLGALVRAAMLDMD
                     LAISVYLDELDGQRQAVQQANETVKREAANAVNALGAGLTKLAGKDLTYRISDDLPDA
                     YTKLKGDFNAAVEQLESAIGAVSGSAEAIHSGTQEISSAADDLSRRTERQAASLEETA
                     AALDEITATVKKSAEGARHAQSVVLAADENAKQSALVVQETIAAMDAIAKSAQQINQI
                     IGVIDEIAFQTNLLALNAGVEAARAGDAGRGFAVVASEVRALAQRSADAAKEIKALIS
                     ASNVQVDHGVKLVGETGKSLERIMAQVGDISDVVSQIATGASEQALGLDQVNATVNEM
                     DRVTQQNATMVEETTAAAHSLSQEMSQLYDFIGKFQIGRVRQERMTDMERKKAAGHVI
                     RNQDGVKPAARFTRGAIKAVVYDEKFRGIARKF"
     gene            complement(1978..2112)
                     /locus_tag="VGB93_09525"
     CDS             complement(1978..2112)
                     /locus_tag="VGB93_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146370.1"
                     /translation="MSKLLTRPEILLLIDGALVYALVLGAGLYATGVRLGIRSRRQRA
                     "
     gene            2159..2314
                     /locus_tag="VGB93_09530"
     CDS             2159..2314
                     /locus_tag="VGB93_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0146371.1"
                     /translation="MKVLWAKSYAATSTEDLLQAMGLGRQSLYNAFGDNRRLYLEALA
                     AYQGRTM"
     gene            complement(2430..2636)
                     /locus_tag="VGB93_09535"
     CDS             complement(2430..2636)
                     /locus_tag="VGB93_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146372.1"
                     /translation="MSDLLERMPPQHRQVFAEIQILMAFPTFGPPSIQSLADTYHPTF
                     QQLLTMPGMSHSLLADLLHRVGVH"
     gene            complement(3001..3078)
                     /locus_tag="VGB93_09540"
     tRNA            complement(3001..3078)
                     /locus_tag="VGB93_09540"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3041..3043),aa:Pro,seq:ggg)
BASE COUNT          705 a          926 c          913 g          590 t
ORIGIN      
        1 ccaagttgag cagatgcgaa acatcgcatc cgctcaaggc cgtcgcgcgg gcgcggacgc
       61 aaaagcgccg ttacgctccg aaaaatctgc agctttccgc aattatgctc caggtcaatt
      121 cgcctgacga ttccccgagt ctttggcagc gctcttgacg acgttcgacg tcgccaagtt
      181 tcgactttgc cacggatgtc tatatttctg tttataagta ttaatgagaa cttaatgggg
      241 taaaaaaacg ttttccatac gaaatatctc cgacacaata ttgatcctaa tcaacagcgg
      301 aacccaattc acgtcataca cagggcgaat caatacattg aaatctgagg ccctgatgtc
      361 tgagtccgtg tatagccggg atgctttgag tgaacgtctc gattttctag ggctcgacga
      421 gcggatgcgc acaacactcc gagacttgac gcctgttctt acgcaggcta tccgccctgc
      481 cctgaacacc ttctatgata aggtccgcgc cacgccgcac atgcgggctt ttttcagcga
      541 cgaccgtcac atcgccgctg cgcaaagcca tcaggaaaag cattgggcgc tgatcgccga
      601 agcaaacttc gatacggatt atacaaatgc tgtccggcgc atcggtcagg cccatgcccg
      661 catcggcctt gaaccccgtt ggtatatcgg cggctacgcg gccgtcctca ccgagcttgt
      721 ccgtaccctc gtcaaggagc aatggccgcg cttgacgcaa ctctcgaagc gcagccccca
      781 aagtgtcgcg gagtccttgg gcgcgctcgt cagggcggca atgctcgaca tggacctcgc
      841 catttccgtc tatctcgatg agctcgacgg gcagcgccaa gcagtgcagc aagcaaacga
      901 aaccgttaag cgcgaggcgg cgaacgccgt caatgccctt ggcgccggct tgaccaagct
      961 tgcaggcaag gatctgacct atcgcatttc cgatgatctt ccagacgcct acactaagct
     1021 aaaaggtgat ttcaacgcgg cggtagaaca gcttgagagc gcaataggtg ctgtctctgg
     1081 aagtgccgag gctatccatt ccggcacgca ggagatttcc tcggccgccg acgatctctc
     1141 gcgccgcacc gagcgacagg cggcaagcct tgaggagacg gcggcggcgc tcgatgagat
     1201 taccgcgacg gtcaagaagt cggccgaggg agcgcgccat gcgcaatcgg tcgtcttggc
     1261 tgctgatgaa aacgccaagc aaagcgccct cgtcgtgcag gaaaccatcg ccgcgatgga
     1321 tgcgatcgcc aaatcggcac agcaaatcaa tcagatcatc ggcgtcatcg acgagatcgc
     1381 cttccaaacc aatctgcttg cgcttaacgc aggtgtggaa gccgcccgcg ctggcgatgc
     1441 cggacgcggc tttgcggtcg tggcttcgga agttcgcgcc ttggcgcaac gttccgccga
     1501 tgccgccaaa gagatcaagg ccctgatctc cgcctccaac gtgcaagtcg atcacggcgt
     1561 gaagcttgtc ggagaaaccg gtaaatcgct cgaacgaatc atggcgcagg tcggcgacat
     1621 cagtgatgtc gtcagccaga tcgcaacggg cgccagcgaa caggcacttg gtctcgatca
     1681 ggtcaacgcc actgtcaacg agatggaccg cgtgacgcag cagaatgcga caatggtcga
     1741 agaaacgacc gcagccgccc attccctgtc acaggaaatg tcacagctct acgatttcat
     1801 cggcaaattt cagatcgggc gagtgcggca ggaaaggatg accgacatgg agcgaaagaa
     1861 agccgccggc cacgtcattc ggaaccagga tggcgtcaag cccgcggcac ggttcacgcg
     1921 cggcgcaata aaggcggtcg tctatgacga aaaatttcgc ggaatcgcgc ggaaattcta
     1981 ggctcgctga cgccgcgagc gaatcccgag ccttacacct gtcgcataaa gtcctgcgcc
     2041 aagcacaagc gcatagacca aagcgccgtc gataagcagc aggatctcgg gccgcgttag
     2101 aagtttactc atagtcaggc cccgaaagtt cgatcgcgat gccgtcctga aacacgccat
     2161 gaaggtgctc tgggccaaga gttatgccgc cacgtcgacc gaggatttgt tgcaagcaat
     2221 ggggctcggc cgtcagagcc tttacaatgc gttcggcgac aatcggcgcc tctatctgga
     2281 agcgcttgcg gcctatcagg ggcgcaccat gtagccggcc accctaagat ggttgagcga
     2341 acctgcctcg ccgtggaagg catctgtggc cttgctctat ggactgatct cagatgatga
     2401 cgccctgcga gccatgggcg gaggctcagt cagtgaaccc ccacacggtg gagcaaatcc
     2461 gcgaggagtg aatgactcat tcccggcatc gtcagcaatt gctggaacgt cgggtggtat
     2521 gtatcggcca agctttggat tgacggcgga ccgaacgtcg ggaacgccat gaggatttgg
     2581 atttccgcga agacttgcct gtgctgtggc ggcatccgct caagcaggtc actcatgccg
     2641 gcctccgctg acattgctgc gccagtcgtc ccggccgcgt tgcagtggtg ggatggctgg
     2701 cccgctgaga ggcggagcgg aaatgaccgt catggcggcg aggtctgccg cgacccgcga
     2761 agtccctgag tcggggcgaa cgaaacccgt ggatttagaa cccgccgggt cgctctgctg
     2821 gcggccgtcg gacctggtcc ggcgaccaga cgcgtcagga tcggcggcgg aacaaacgcg
     2881 atactttgcg cgggactttt cgccgccgaa tacacgcttc cgaggggttt tgttcccatt
     2941 tggggagaac gggcttcagc cctcccgaga caggaaatca aaataaatca ataagttgag
     3001 tggtcggagt ggcgggattc gaacccacga ccccttgtcc cccagacaag tgcgctaacc
     3061 gggctgcgct acactccgac tgcggctctt atagagaaac gccgagaccg ctgcaacgcg
     3121 catccggccc gaaa
//
LOCUS       DASWOG010000462         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_175927,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000462 DASWOG010000000
VERSION     DASWOG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175927"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VGB93_09545"
     CDS             <1..631
                     /locus_tag="VGB93_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0146373.1"
                     /translation="VKADAYLNTIHDYIDFEPVGGTFLTSGIPGGIPGIPAGELCQFI
                     PNACFPITNFQYLNIGEAQISGVELEGTYDWGGGYVTVAGTHINGKNVSDPSTPLFSI
                     PPDRISGTLGLRFLDRKLIVGTRLTLVDASQQFPFSTGEDSSNGSTFPPTAGYALVDL
                     FASYKYNDDISASLTVKNLLNKEYTQYLDNEANPGLQVLGSVTVRFASK"
     gene            681..1022
                     /locus_tag="VGB93_09550"
     CDS             681..1022
                     /locus_tag="VGB93_09550"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0146374.1"
                     /translation="MSNYIAMNRFKVIKDQAHAFEQVWLTRVSRLDEVPGFIEFHLLR
                     GPEHEDYVLYASHTIWANKETFTAWTNSEQFRAAHARAGDEKPKTLGHPEFEGFEVIQ
                     TVDNPKRHAAE"
     gene            1159..1617
                     /locus_tag="VGB93_09555"
     CDS             1159..1617
                     /locus_tag="VGB93_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0146375.1"
                     /translation="MVQFFALISAGFAMAGPIKIENAFTRPTLGGASVAVGYMMITNT
                     GKIDDRLESVTSDISATSEIHETKMENGMMEMRELPTGLAVPAGATVAFKPSAYHVMF
                     VGIKHAVKPGDTVHAILTFDKAGKIAVDFPAADNPGAVAPSSGMGGMKMQ"
     gene            complement(1659..>2100)
                     /locus_tag="VGB93_09560"
     CDS             complement(1659..>2100)
                     /locus_tag="VGB93_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0146376.1"
                     /translation="QQLPTVNLTNNQLGATVLAGVSVPLYDGGMRLAQLKQAQANSDN
                     AKLALLRTRDEAVRQIVLADNALHTSLAAYSASVQLSSAAQTTFDAALAAYRNGVGSI
                     TDATLAETQLLQAQDARSDAYSTALSAAATLALSAGALGSAPGP"
BASE COUNT          435 a          587 c          640 g          438 t
ORIGIN      
        1 tgtgaaggcg gatgcttatc tcaatacgat ccacgattac atcgatttcg agccagttgg
       61 cggcactttc ctgacatccg gcattccggg tggcattccc ggtattccag caggggagtt
      121 gtgtcagttc attccaaatg cctgcttccc gatcaccaac tttcaatatc tcaatattgg
      181 tgaagcacag atttccggtg tcgagcttga aggcacctat gactggggtg gcggctacgt
      241 cacggtggcg gggacgcata tcaacggcaa gaatgtatct gacccgtcga cgccgctctt
      301 ttccattccg ccggaccgca tcagcggcac gctgggcttg cgcttcctcg atcgcaagct
      361 gatcgttgga acgcgcctga cgcttgtcga tgccagtcag cagttcccgt tctcgacggg
      421 cgaagattcg agcaacggct caacatttcc gcccacggca gggtatgcgc tggtcgatct
      481 cttcgcgtct tacaagtata atgacgacat cagcgcaagc ctgacggtca agaaccttct
      541 gaacaaggaa tacacgcaat atttggacaa cgaggccaat ccggggctac aagttctcgg
      601 ctcggtgacg gtccggttcg catcgaaatg atataagtag gggcgctcta ttagcccgcg
      661 gcagtttgga ggataaacgc atgtcgaact atatcgctat gaaccgtttc aaggtcatca
      721 aagatcaggc tcacgccttc gagcaggtgt ggctcacgcg cgtatcgcgt ctcgacgaag
      781 tcccgggttt catcgaattt catctgctgc gaggcccgga gcacgaggat tatgtcctct
      841 atgcttcgca cacgatctgg gcgaataaag agactttcac cgcgtggacg aattcggaac
      901 agtttcgcgc ggcgcatgcg cgcgccggcg acgagaagcc gaagacgctc ggccatccgg
      961 agttcgaggg ctttgaagtc attcagacgg tggataatcc caaacggcac gcggcggaat
     1021 aagcggtctc gcacgtgtga atggtgccgc gcctcttgcg cttgaccggg gttgaatcgt
     1081 cgctagattg cggacgcgat gagagcaaga ttacaacgcc gatttgaatg gacagcgccg
     1141 acttttttga tcgcgctgat ggtgcaattc tttgcgctca tcagcgccgg cttcgccatg
     1201 gctggcccga tcaagatcga aaatgctttc acgcgaccga cgctgggcgg tgcgagtgtc
     1261 gctgtcggct atatgatgat tacgaacacc gggaagatag acgaccggct agaaagcgtg
     1321 acctcggaca tttccgcgac atccgagatc catgaaacga aaatggaaaa cggcatgatg
     1381 gagatgcgcg aattgccgac agggcttgcc gtccccgcgg gcgccaccgt cgcctttaag
     1441 ccgagcgcct atcatgtcat gtttgtcggc atcaaacatg ccgtcaaacc cggcgacacg
     1501 gttcacgcaa tcctgacctt cgacaaggcc ggcaagatcg ctgtcgattt tccggcggcg
     1561 gacaatccgg gtgccgtggc gccaagcagc ggcatgggcg gcatgaaaat gcaatagcgg
     1621 ccgctgccgc tggagcatcc tcgccaacgc tcgattcgtc atggtcccgg ggcgcttccg
     1681 agcgcgccgg cggacagggc caaagtcgcg gcagcggaga gcgcagtgct gtaggcgtca
     1741 ctgcgggcat cttgcgcctg cagaagttgc gtctcggcga gagtcgcgtc ggtgatcgag
     1801 ccgacgccat tgcggtaggc ggcgagcgct gcatcgaacg tcgtctgcgc cgcactcgag
     1861 agttgaaccg aggcgctata ggccgccaga cttgtatgca gcgcattgtc ggcgaggacg
     1921 atctgacgga ccgcctcatc gcgggtgcgt agcagggcga gtttcgcgtt gtcggaattg
     1981 gcctgcgcct gtttcagttg ggcgaggcgc atgccgccgt catagagcgg gacgctcacg
     2041 ccggccagga cggttgcacc caattggttg ttcgtgagat tgacagtcgg caattgctgt
//
LOCUS       DASWOG010000463         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_157385,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000463 DASWOG010000000
VERSION     DASWOG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157385"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1916)
                     /locus_tag="VGB93_09565"
     CDS             complement(78..1916)
                     /locus_tag="VGB93_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HEY0146377.1"
                     /translation="MSEPKHHTKDLSLSRLYFRALGLLGTETHVAIFLAFANFALAAA
                     QFAEPVLFGRIIDRMNAGEAQNQSSGLHALAPLIGLWIGFALFSIIGAVFVGLRADRM
                     AHRRRLAAMGIFFEHVLNLPLSFHASVHSGRLLKGMLEGASALFWLWLSFFREKCADF
                     VALTVLLPLALFINWRLGLPLIILVVIFGGIIIFVVRRTHTLQTQVEGFNANLSERAS
                     DVLGNIAVIQSFTRIQSEASAMNQLIQAVLNAQMPVLSWWAVAVVAARAASTLTLLTI
                     FIVGVWLHQQNLATIGQLVTFMALATMLIGKLVEIVNFINGLFLQAPKLSEFFNVLDT
                     SPGVADRPDAQDPGKLKGHVVFNHVTFAYVDGKIAIENVSFDVPAGETIALVGATGSG
                     KSTTLSLLHRVFDPQSGTISIDGIDIRDMTLAGLRRNIGVVFQEPMLFARSIEENVKI
                     GNLDASDSDVEQALNLAQADEFVARQTDGLATIVGERGRSLSGGERQRLSIARALLKN
                     PPIMIFDEATSALDVETERKLQIAMARATKGRTTFIIAHRLATVRNADRIFVFDRGEI
                     VERGSYEELLAQGGRFAKLAETQNVLQTPESDFEAVSSHGARPEEV"
     gene            2014..>2213
                     /locus_tag="VGB93_09570"
     CDS             2014..>2213
                     /locus_tag="VGB93_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146378.1"
                     /translation="MNTGLTEYDEIVAERFAFVVEVETAVRRLDALLAARPEALEASL
                     SRTRLKALIEAGAVEIDGVPAL"
BASE COUNT          456 a          630 c          741 g          386 t
ORIGIN      
        1 gcgcgatcat gggcaggatg gagccgcgca tgatgaagag caggctgaac gcgttggggc
       61 ggggacggac gatcaattca aacctcttcc gggcgtgcgc cgtggcttga gacagcctcg
      121 aaatctgatt ccggcgtctg caacacgttt tgcgtctccg cgagtttggc gaagcgtccg
      181 ccttgcgcga gaagctcttc gtagctgccg cgttcgacaa tctcgccgcg atcgaaaacg
      241 aagatgcggt cggcgttgcg caccgtcgcg agccggtgcg cgatgatgaa ggtcgtgcgc
      301 cccttcgtcg cccgcgccat ggcgatctgc aatttccgct cggtttcgac atcgagtgcc
      361 gaggtggcct cgtcgaagat catgatcggc gggtttttca aaagcgcccg ggcgatcgaa
      421 aggcgctggc gttcgccacc cgacaacgac cggccgcgct cgccgacaat cgtcgcgaga
      481 ccgtcggttt ggcgcgcgac gaactcgtcg gcttgggcaa ggttcaacgc ctgctcgaca
      541 tcggaatcgc tcgcgtcgag attgccgatc ttgacgtttt cctcgatcga gcgggcgaat
      601 agcatgggct cctgaaagac gacgccgatg ttgcggcgca ggcccgcgag cgtcatgtcg
      661 cgaatgtcga tgccgtcgat cgagatcgtt ccggattgcg gatcgaagac ccggtgcagc
      721 aggctgagcg tggttgattt accggagccg gtcgcgccga caagcgcgat cgtctcgccc
      781 gccggaacgt cgaagctgac gttctcgatc gcgatcttgc cgtccacata agcgaaggtc
      841 acgtggttga agacgacatg gcccttgagc ttgcccggat cctgggcgtc cggccggtcg
      901 gcaacgccgg gcgaggtgtc gaggacattg aagaattcgg acagtttcgg cgcctgcaga
      961 aacagtccgt tgatgaaatt gacgatctcg accagcttgc cgatcagcat cgtcgcgagg
     1021 gccatgaagg tcacgagctg gccgatcgtc gccaggtttt gctgatgcag ccagacaccg
     1081 acgatgaaga tcgtcaagag cgtcaatgtc gaggcggcgc gtgccgcgac aaccgccact
     1141 gcccaccagg agagcacggg catctgcgcg ttgagcacgg cctgaatcaa ctgattcata
     1201 gccgaagctt cgctctggat gcgggtgaag ctctggatca cggcgatatt gccgagcacg
     1261 tccgaggcac gctcggacag attggcgttg aagccctcga cctgcgtctg cagcgtgtgc
     1321 gtgcggcgga cgacgaagat gatgatgccg ccgaaaatga cgacgaggat gatgagcggc
     1381 agcccgagcc gccagttgat gaagagcgcc agcggcagca gcaccgtcag cgcgacgaaa
     1441 tcggcgcatt tttcgcggaa gaacgatagc cagagccaga agagcgccga agcaccttcg
     1501 agcatcccct ttagcagccg cccggaatgg actgaggcgt ggaacgagag gggcaggttg
     1561 aggacgtgct cgaaaaaaat gcccatcgcg gccaaccggc gccggtgcgc catccggtcg
     1621 gcacgcaggc cgacgaaaac cgcaccgatg atcgagaaga gcgcaaagcc gatccataaa
     1681 ccgatcagag gtgccagcgc gtggaggccg gagctttgat tttgtgcttc gccggcgttc
     1741 atccggtcga tgatgcggcc gaagagaacc ggctcggcga attgcgccgc ggcgagcgcg
     1801 aaattggcaa aggcgagaaa gatcgccaca tgtgtctcag tgccgaggag gcccagggcg
     1861 cggaaataaa gccgcgacag cgagagatcc ttggtgtggt gcttgggctc ggacatgcgc
     1921 tagagatcaa ccggataggt gccgcgacga gggcattttt tgacatcgtt caaatgaggt
     1981 catgccccag tggcgaagca aacagcaatt gaaatgaaca ccggcttgac ggaatacgac
     2041 gaaattgtcg cggagaggtt tgctttcgtc gtcgaggttg agacggccgt gcggcgactc
     2101 gacgcgctcc ttgccgcgcg cccagaggcg ctggaagctt cgctgtcgcg cacacggctg
     2161 aaggccttga ttgaagccgg ggcggtcgag atcgacggcg tgccggcgct cga
//
LOCUS       DASWOG010000464         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_212295,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000464 DASWOG010000000
VERSION     DASWOG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212295"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..744)
                     /locus_tag="VGB93_09575"
     CDS             complement(46..744)
                     /locus_tag="VGB93_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146379.1"
                     /translation="MTTLSAPLRVLVVDDEPAILRFLRPSLATQGYVVSEAKDGRSAL
                     DAIRAKAADVVVLDLGLPDMDGLEIIRQLRGAGETLPIIVLSSRETEGAKVAALDLGA
                     DDYVTKPFGIDELLARIRAAQRHRLQQQGEKAIFRNGDLTADLVRRIITVRGQEVKFS
                     PREYDVLRLLIAHAGKVLTHRFILREVWGGDADVQYLRIYIRALRQKIERDPERPRHI
                     LTETGVGYRLALQE"
     gene            complement(741..>2469)
                     /locus_tag="VGB93_09580"
     CDS             complement(741..>2469)
                     /locus_tag="VGB93_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0146380.1"
                     /translation="GEDAAPMLLDYAHTHNATHIRVAQTGRASWRDILRGSTLNKLIQ
                     NAGGINIHIATADSAAGKTRRFAFVAPDSPRDWTVYLKSLVFVAGAGAFAFLLRETLG
                     ISSVALIFLIGVLASAVCYGLFPSLVAALASALAYNFFFLPPLYTFTIADPENVVALT
                     TFAIVAVIASNLAARLRTQAIAARARARITEELYLFSRKLAGAASLDDIIWASVYEIA
                     LLLKVNVVLLLPRNGKLEPMGGYPPEDRLDEADFAAAQWSFERNHAAGRGADTLPGAK
                     RLFIPMRTGRGAIGVIGIDSERPGPLLRPEESRLLDSLSDQAALAIERIHLAADIERA
                     RLAEETDRLRQALLTSISHDLRTPLASVIGSATSLVAHGAELDRATQISLARTIQEEG
                     ERLNRFIGNLLDMTRLESGSLKPNSDLVDVSDLVGAVLQRGQKILKDHKVEIAVAPEL
                     PMLSLDVVLTEQVLFNLLDNAAKYAPPGTKITLGAHRENGNVLITVTDEGEGIPPADY
                     EQIFNKFYRVHRGDHQRAGTGLGLAICRGFVEAMSGTITAANRTDRSGAIFTLKFPVP
                     PPSNIEAIK"
BASE COUNT          496 a          722 c          784 g          467 t
ORIGIN      
        1 cttgctgccc cgccgcccgg gaacgtcgcc tgatgaacag ccattctact cttgtaaggc
       61 gaggcgatag ccgacgccgg tttcggtgag gatgtggcgc gggcgctcgg ggtcacgttc
      121 gatcttctgg cgtagagcgc ggatgtagat gcgcagatat tggacgtcgg catcgccgcc
      181 ccagacttcg cgtaggatga agcgatgtgt caaaaccttg ccggcatgcg cgatcagcaa
      241 tcgcagcaca tcgtattctc gcggtgaaaa tttcacctcc tggccgcgga ccgtgatgat
      301 ccggcggaca agatcggcgg tcagatcgcc gttgcggaaa atcgcttttt caccttgctg
      361 ctggaggcgg tgacgttgcg cggcgcggat gcgcgccagc aattcgtcga tgccgaaagg
      421 cttggtcacg taatcgtccg cgcccagatc aagcgccgcg acctttgcac cctccgtctc
      481 acggcttgat agaacgatga tcggcagagt ttcgccggca ccgcgcaatt ggcggataat
      541 ctcgagtccg tccatgtcgg gcaggccgag gtcgaggacg acgacatcgg ctgccttcgc
      601 gcggatcgcg tcgagtgcgc tgcgcccgtc cttcgcttca gagacgacgt agccctgtgt
      661 cgccagactt ggccgcagaa agcgcagaat cgccggttcg tcatcgacga ccagcacgcg
      721 caggggcgcc gaaagtgtcg tcatttaatc gcctctatat ttgacggggg tggcacagga
      781 aatttgagtg tgaagatcgc gccgctccgg tccgtgcggt tggccgccgt gatcgtgccg
      841 ctcatggctt cgacaaagcc gcggcagatg gcgaggccaa ggccggtgcc ggcgcgctgg
      901 tggtcgccgc gatgaacgcg atagaacttg ttgaaaatct gctcataatc ggcgggcgga
      961 ataccttcac cctcgtcggt gaccgtaatc agaacattgc cgttctcgcg gtgcgcgccg
     1021 agcgtgatct tggtaccagg cggtgcgtat tttgcggcgt tgtcgagcag gttgaagaga
     1081 acctgctcgg tcagcacgac atcgagcgag agcatcggca gttccggtgc gacggcaatt
     1141 tccactttat ggtctttcag gattttctgc ccccgttgga gcacggcacc gacgagatcg
     1201 gagacatcca ccagatcgga attgggtttg agcgaccccg actcaagccg cgtcatgtca
     1261 agcaaattac cgatgaaccg gttcaaccgc tcgccttctt cctgaatagt ccgcgcgagg
     1321 cttatctgcg tcgcgcggtc gagttcggcg ccatgcgcga caaggcttgt cgcggatccg
     1381 atgaccgacg cgagtggcgt gcgcagatca tgcgagatcg atgtcagcaa cgcctgccgc
     1441 agccggtccg tttcctcggc gagccgggcc ctttcgatat cggccgcgag atggatgcgc
     1501 tcgatcgcca gcgccgcctg atcggacagt gaatcgagca gacggctttc ttcgggccgc
     1561 aacagtggac ccggacgttc tgaatcgatg ccgatcacgc cgatggcgcc acgtcccgta
     1621 cgcatgggaa tgaagagccg cttggcgccg gggagcgtat cggcgccgcg accggcagcg
     1681 tgatttctct cgaaggacca ttgcgccgcc gcgaaatccg cttcgtccaa gcgatcctcg
     1741 ggtggatagc cacccatcgg ctcgagtttg ccgttgcgcg gcaacaataa aacgacgttt
     1801 actttcaaaa gcagcgcgat ctcgtagacg ctggcccaga tgatatcgtc gagcgacgca
     1861 gctccggcca gcttgcggct gaaaagataa agctcttcgg taatgcgggc ccgcgcccgc
     1921 gccgcgatcg cttgcgtccg caaacgcgcc gcgagattgc tggcgatcac cgcaacaatc
     1981 gcgaaagtcg tcagcgcgac gacattttcg ggatcggcaa tcgtaaacgt ataaagcggt
     2041 ggcaggaaaa agaaattata ggccagcgca ctggccaacg cggcgacaag agacgggaag
     2101 aggccgtagc agacggcgct tgccaacacg ccaatgagga aaattagcgc aacgctcgaa
     2161 atgccgagcg tctcgcgcaa caggaaggcg aaagcaccgg cgccagcgac gaagacgagg
     2221 ctcttaagat agacggtcca atcgcgcgga gaatcgggcg ccacaaacgc aaaacgtcgt
     2281 gttttgccgg cggcggaatc cgctgttgcg atgtgaatgt tgatgccgcc ggcgttctgg
     2341 atcaatttat tgagggtcga gccacgcaga atgtcgcgcc agcttgcccg gccggtctga
     2401 gcgacacgga tatgcgtggc attgtgcgta tgggcgtagt cgaggagcat cggtgcggca
     2461 tcctcgcca
//
LOCUS       DASWOG010000465         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_103075,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000465 DASWOG010000000
VERSION     DASWOG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103075"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB93_09585"
     CDS             <1..186
                     /locus_tag="VGB93_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0146381.1"
                     /translation="WLAARVDEDTFFIFDAFNAESGRNAHLNGPIATALMANAEKLLA
                     TAPVIHKADVLADKLPA"
     gene            222..1226
                     /gene="dusA"
                     /locus_tag="VGB93_09590"
     CDS             222..1226
                     /gene="dusA"
                     /locus_tag="VGB93_09590"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY0146382.1"
                     /translation="MTKTIDELSHRFCVAPMMDWTDRHCRVFHRLLSRRARLYTEMVT
                     AAAIMHGDRQRLLGFDAQEHPLAVQLGGSEPVMLAEAAVIAADFDYDEINLNIGCPSE
                     RVQNGAFGACLMREPDLVADCVAAMKARVRVPVTVKCRIGVDEQEPEALSIFAGRVIA
                     AGADLLIVHARKAWLKGLSPRENRSVPPLDYARVRALKNEYPNVPIVLNGGLIEIGQM
                     QAELEHLDGVMVGRAAYQDPAILMTVDSALFGEPAPMSSRHAALEAFMPYIEARLAEG
                     VSLSSMTRHVLGLYNGLPGARAFRRHLATEAVKPGAGLDVLRAAMNFVPEHAIAEAAE
                     "
     gene            1223..2002
                     /locus_tag="VGB93_09595"
     CDS             1223..2002
                     /locus_tag="VGB93_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146383.1"
                     /translation="MNARDMRDAVASKVPAVTLVFWIIKIAATTLGETGGDSVTMTLN
                     WGYLAGTVLFLTALIVLVALQIAAKKFHPWLYWATIVASTTAGTTMADFADRSLGIGY
                     VGGSTLLSVCLMAVLALWYWTQGSVAVETVTTPKVEAFYWAAITFSQTLGTALGDWMA
                     DSGLGYAGGALVFAASLLILIGLYYWTQISRVFLFWAAFILTRPLGATFGDFLDKPLN
                     HGGLAFSRPLASLILAVFIVGCILILPQRAGTHPGEAHATS"
     gene            complement(2007..3107)
                     /locus_tag="VGB93_09600"
     CDS             complement(2007..3107)
                     /locus_tag="VGB93_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0146384.1"
                     /translation="MGDIDHRLLSEAVSQFYEAAMTPAFWPGALKTLSNAFNGSGSLF
                     LYPQQSGVPWHACSPGLEESVDVFFRESWYNQNFRMAGREFMARNNLKIFTETDLLTP
                     AQLDLQPIQADFFDRFDLRSFLAFNLGPIQASIERGKEPFTTEQASTLNKVLPHLQQA
                     GRLATAVGIARNEGALETFTTMRTGAALLDYAGRVTLMNSEAERLLNSAARLQGGVII
                     PHDRDATAALRRMIGMATSPGALLGGDLTEMVALKRPQGWPLFLRIAPLVNARADMFQ
                     MAKAILTIVDPYDRPDIAHDRLMRLLGLTPAEARVAKELARGATLAEIADEFNLSMKT
                     VRTQLRSIFAKTETHRQSDLIALLTRIFLTPH"
     gene            complement(3504..4604)
                     /locus_tag="VGB93_09605"
     CDS             complement(3504..4604)
                     /locus_tag="VGB93_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HEY0146385.1"
                     /translation="MDALKNFLHKEWLLFVSVATVAAFWHWGREIFAGLSNPLWLAFV
                     FIWLFSVVLASALAVVRHADALAERLGEPYGTLILTLSVTAVEVMSISAVMLHGANNP
                     TLVLDTLFAVVMIILNGMVGLSLLVGGLRHAEQHYNLQGANAFLGVIIPLAVFSLIMP
                     DFTQTTPGPRLSAHQQIFLIVMSAGLYCAFLVIQTGRHRGFFTLGDEPIEHFAKVREP
                     IFPHAVLLLAYMIPVVSLAEKLAHPIDYLVETQHFPDALGGLVIALLVATPEALGAAK
                     AAMRNHLQRSMNIFLGSVLSTIGLTIPAMIVMSHITGRGLDLGLEHTDFVMLLLTLGV
                     SVVTFASGRTNILQGIVHLLLFLSYVLLLIEG"
BASE COUNT          944 a         1367 c         1456 g          981 t
ORIGIN      
        1 tggttagcgg cacgcgtcga tgaagacacg tttttcattt tcgacgcttt taatgctgaa
       61 tccggacgca acgcccatct gaacggcccg atcgcgacag ctctcatggc caatgccgag
      121 aagcttctcg cgacggcgcc cgtcattcac aaagcggatg ttctggcgga caaattgcct
      181 gcctgatata ggctggcgca ttccttgtga atgagccaac gttgacgaag acgattgatg
      241 aactgagcca ccgtttctgc gtggcgccga tgatggattg gacagaccgg cattgccgtg
      301 ttttccatcg tctattgtcg cgccgcgcgc ggctttatac cgagatggtg acggccgcgg
      361 cgatcatgca tggcgaccgc cagcggctcc tcggtttcga cgcgcaggaa catccgcttg
      421 ccgtgcagct tggcggttcg gaaccggtga tgctcgccga agctgccgtg atcgcggccg
      481 atttcgacta tgacgagatc aatctcaata tcggctgccc atcggagcgc gtgcagaacg
      541 gcgcgttcgg ggcctgcctc atgcgcgagc ccgacctcgt cgccgattgt gtcgcggcaa
      601 tgaaagcgcg cgttcgcgtg cctgtcaccg tcaaatgccg gatcggcgtg gacgagcagg
      661 agccagaggc cttgtcgatc ttcgcagggc gggtgatcgc ggccggtgcc gatctgctca
      721 tcgttcatgc ccgtaaggcg tggctgaaag gtttgtcgcc gcgcgagaac cgcagcgttc
      781 cgccattgga ttacgcgcgc gtgcgcgcct tgaagaacga atatccgaat gtgccgatcg
      841 tgttgaacgg cggcttgatc gagatcgggc agatgcaagc cgaactcgaa caccttgacg
      901 gcgtgatggt cggccgtgct gcgtatcagg acccggcgat actcatgacg gtcgattcgg
      961 ccctcttcgg cgaacccgca cctatgtcga gccgccatgc cgcgctcgaa gctttcatgc
     1021 cctatatcga agcacgcctc gctgaaggcg tgtcgctctc ttcgatgaca cggcacgttc
     1081 tcgggcttta caacggcctg ccgggcgcac gcgccttccg ccgccatctc gccacagagg
     1141 cagtgaaacc tggcgcggga ctagatgtgc tgcgcgccgc gatgaacttc gtgccggagc
     1201 acgcgattgc ggaggccgcc gaatgaacgc cagagacatg cgcgatgctg tggccagcaa
     1261 agtgcccgct gttacgcttg tcttctggat catcaagatt gccgcgacga cgcttggcga
     1321 gacgggtggc gatagcgtca cgatgacatt gaactggggt tatctcgccg gaaccgtgtt
     1381 gtttcttacc gcgctcattg tgcttgtcgc gctgcaaatt gcggcgaaga aatttcatcc
     1441 ctggctctat tgggcgacga tcgtcgcttc gacgacagcg ggcacgacga tggcggattt
     1501 cgctgaccgt tccctgggta ttggatatgt tggcggttcg acgctgcttt ctgtctgtct
     1561 gatggccgtg cttgcgctct ggtactggac acaaggttcg gttgccgtcg agactgtgac
     1621 cacgccgaaa gtcgaagcct tctattgggc ggcgatcaca ttctcacaga cgctcggcac
     1681 agcgttgggc gattggatgg ccgatagcgg tcttggttat gcaggcggcg ctttggtttt
     1741 cgcggcgtcg cttttgatcc tcatcgggct ttattattgg acgcagattt cgcgcgtctt
     1801 tttgttctgg gccgctttca ttctgacgcg gccgcttggt gcgacattcg gcgactttct
     1861 cgacaagccg ttgaaccacg gcgggctcgc gttcagccgt ccgctggcct cgctcattct
     1921 ggcggtcttt atcgtcggct gcatccttat cctgccgcaa cgggccggaa cgcatccggg
     1981 cgaggcacac gcgacgtcgt agcgtatcaa tgcggtgtca ggaaaatccg tgtgagcaat
     2041 gcgatcagat ccgattgtcg gtgtgtctcc gtcttggcaa agattgagcg caattgcgtg
     2101 cggactgtct tcatcgacag attaaattcg tcggcgatct cagccaatgt cgcgcctctc
     2161 gcaagctctt tggcgacgcg cgcctcggcc ggtgtaaggc cgagcagccg catgagacgg
     2221 tcgtgggcga tatccggacg atcgtaggga tcgacgattg tgagaatggc ttttgccatt
     2281 tgaaacatat cggcgcgcgc gttcacgagc ggcgcaatgc gtagaaacag tggccagccc
     2341 tgcggtcgtt tcaaagcaac catttccgtc aaatccccgc ccaacagggc cccgggcgag
     2401 gtcgccatac cgatcatgcg gcgcagtgcg gccgtcgcat cgcgatcgtg cgggatgatc
     2461 acgccgcctt gcaagcgcgc agcgctgttg aggagccgct ccgcctcgga attcatgagc
     2521 gtgacgcggc cggcatagtc cagcagtgct gcgccggttc gcatcgtggt gaaggtttca
     2581 agggcccctt cgttgcgcgc gatgcccacc gccgtggcga gccgtccggc ctgttggaga
     2641 tgcggcagca ctttattcag ggttgacgcc tgctctgtcg tgaacggctc cttaccgcgt
     2701 tcgatagacg cctggatcgg tcccaggttg aaagcgagaa agctgcgcag gtcgaagcga
     2761 tcgaaaaaat ctgcctgaat gggctgaagg tccaattgtg ccggtgtgag cagatcggtt
     2821 tcagtgaaaa tcttgagatt gttgcgggcc atgaattcgc ggccggccat gcggaaattc
     2881 tggttgtacc aactttcgcg gaagaaaacg tcgacactct cttccaaacc gggcgagcat
     2941 gcgtgccagg gcacccctga ttgctgcggg taaagaaaca agctgccgga tccattgaag
     3001 gcattcgata gtgtcttgag tgcccccggc cagaacgccg gtgtcatcgc agcttcataa
     3061 aactggctga cggcctcgga gagcaaacga tgatcgatat cacccacgct tcgcctcctt
     3121 gaaattccgg tcatcgcaaa gtgccagaca gcgcagggca tgcaggacat tgcaacgcgt
     3181 aattgactgt gcggagccaa gcgtcgcggc atcttagccc gttgcgttgc aggtatgcaa
     3241 cgccgcagag ccaaactggc ccggcatacc tcatttgccc gaagcggcct cgttctcggt
     3301 atgccagaat gctaaggcgc accacgagtt acgggtcgct tcatcgctgg cagacggcaa
     3361 tacgttttag ttagaaattt tcagacagac cctttgcacg agataaattt tttatgcggc
     3421 gccgattcca gtgatcggcg ccgatttact tttgtcttca aatttgcgcg ccgcccaatt
     3481 ggtttgcggc cgccacggcg ctttcagcct tcgatcagca aaagcacata ggacagaaac
     3541 agcagcaggt gcacgatacc ttgcaggata ttcgtccggc cgctcgcgaa ggtgacgacg
     3601 ctcaccccca gcgtcagcag cagcatcaca aaatcggtgt gctcgagacc gagatccaag
     3661 ccgcggccgg tgatatggct catgacgatc atcgccggaa tggtgaggcc gatggtcgag
     3721 agcaccgaac ctagaaagat gttcatcgat cgttgcagat gattgcgcat ggctgccttc
     3781 gcagcaccca gcgcttcggg cgttgcaacg agcagtgcga tgacgagccc gcccagggca
     3841 tccgggaaat gctgcgtctc aaccagatag tcgatgggat gcgcgagctt ttcggcgagc
     3901 gacacgaccg ggatcatata ggcgagaagc agcaccgcat gcggaaagat cggctcccgc
     3961 actttggcga aatgttcgat cggttcatcg ccgagggtga agaaaccgcg atgccgtccg
     4021 gtctggatca cgaggaacgc gcaataaagt cccgccgaca tcacaatcag gaaaatctgc
     4081 tgatgcgccg ataagcgtgg ccctggcgtg gtctgggtga aatcgggcat gatgaggctg
     4141 aagacggcga gcgggatgat gacgccgaga aaagcgttcg cgccctgcag attgtagtgc
     4201 tgttccgcgt ggcgcagccc gccgacgagg agcgagaggc cgaccatgcc gttgaggatg
     4261 atcatcacga ccgcgaacag tgtgtcgagc accagcgtcg ggttgttcgc cccgtgcagc
     4321 atcaccgcgg aaatgctcat cacctcgacc gcggtgactg agagggtgag gatcaacgtg
     4381 ccgtaaggct cgcccagccg ctcggccagc gcatcggcat ggcgcacgac ggcgagcgcc
     4441 gaggcaagga cgacgctaaa aagccagata aagacgaacg cgagccaaag cggattggat
     4501 agccccgcaa agatctcgcg tccccagtgc cagaaggccg ccaccgtcgc gacgctgacg
     4561 aacaacagcc attctttgtg gagaaagttt ttcaatgcgt ccatgaagcg gccgaccccc
     4621 atttgccgcg gagaattagg ctagttagcc cgggggccgg aacagcggca agctgatgcc
     4681 tgcagatgag agtggattat cgctcctcca ccgcatccct gagccgctca atcgttgtct
     4741 gcatctcg
//
LOCUS       DASWOG010000466         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_157430,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000466 DASWOG010000000
VERSION     DASWOG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157430"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..441
                     /locus_tag="VGB93_09610"
     CDS             160..441
                     /locus_tag="VGB93_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146386.1"
                     /translation="MGASQVEAKPGGCLKYGVAGAVAGHYAGHHAMKGAIAGCLAGMA
                     RRKVYNDELARQKADAAKKNATITGAPVESPPPTTSDAPSTATPATDQK"
     gene            complement(432..1448)
                     /gene="galE"
                     /locus_tag="VGB93_09615"
     CDS             complement(432..1448)
                     /gene="galE"
                     /locus_tag="VGB93_09615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0146387.1"
                     /translation="MAILVTGGAGYIGGHMVLALLDAGEQVVVLDDLSTGFAWAVPSA
                     ATLVVGDISDEAKLRAIAAAHHIDAIAHFAAKIVVPESVSDPLGYYLNNTVKARALLE
                     FAVRNGIGKFIFSSTAAVYGEPAINPIFEEAPAAPINPYGRSKLMVEWILEDAARAHG
                     LSYVALRYFNVAGADPKGRLGQSTPNATHLIKVAVQAALGMRSGLDIFGTDYATADGT
                     CVRDYIHVTDLIAAHLLALRHLRAGGESLVLNCGYGHGHSVREVVDVVKNVSGVDFET
                     RLSPRRPGDPAALVAGADRIRAKLGWTPQYDDLREIVNQAYAWERKLRDGTIPKARSA
                     SAYF"
     gene            1639..2535
                     /locus_tag="VGB93_09620"
     CDS             1639..2535
                     /locus_tag="VGB93_09620"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0146388.1"
                     /translation="MSQRVRKAVFPVAGLGTRFLPATKSVPKEMLTVVDRPILQHVVE
                     EAFEAGIEHCIFVTGRNKAVIEDYFDMAYELEDILLKRGKTKEWQALKADLPKPGAMS
                     FTRQQAPLGLGHAVWCARDIVGDEPFAVVLPDMLTMPRRTGGKRCLAQCVEAFETHGG
                     NVIAVEEVKPEETHKYGVVSVGDKSGSTFEITGMVEKPPQGTAQSNFIISGRYVLSPE
                     IFSILERGEKGSGGEIQLTDGMKSLAHDQKFHGVNFDGQTYDCGSSLGFLGANVAFAL
                     ASPDIAPEFRALVKSLLNAPQD"
     gene            complement(2532..4067)
                     /locus_tag="VGB93_09625"
     CDS             complement(2532..4067)
                     /locus_tag="VGB93_09625"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0146389.1"
                     /translation="MQAPPLPPPELRGSEGPALNDLPGATISPDSDDLNPVAAPPANY
                     GLPAPVTASGRVTNYGRPRPKKSALYQLPKIRRVGQRPLPLLRAYATAAAPALRRRSI
                     ADLPADPGPTVAVIPILPQPLRPKADLTPFDAIGVGVGSLRLYPYVETATGYDSNPNL
                     LNTDVKGSPYIYGETGLKLQSDWSQSSLAADLHGGYYDYLTDPTANQPNATGTITGRI
                     DVTRQSQINLQTTFSLTEGTPGSPLLSVPNAVFVTSRPLYASIGQTLGFTQQFNRLSV
                     SINSAFAHYFFGNATQSDGTDLQLALNNYNAYSVAGRVSYELTPALIPYVQVTGTRNQ
                     YDSSMDVDGFDRSSNGVLAQVGTTYEVTRLLTGDISVGYDQRFYADPRLPVARAPTVN
                     ASLIYDATPLTTLTFTAATDVGETTLANSSAVVAHTLSLKISHDLLRNLTLAATGTYQ
                     INNYIGEPEIDQFYSGLFEADYHMTRSIMITGSYKYQRYTSTAGFTNYIDHVFLAGLK
                     FQL"
     gene            4482..>4586
                     /locus_tag="VGB93_09630"
     CDS             4482..>4586
                     /locus_tag="VGB93_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HEY0146390.1"
                     /translation="MTLLLIIVVLILLFGGGGGYYGYKRGYYGGNGLGC"
BASE COUNT          986 a         1302 c         1373 g          925 t
ORIGIN      
        1 tgaccgcgtg cgccggcaca cgcgagcgcg tttgcgctcg tcgtgaccgt caccatattt
       61 cgcctgcccg gccacatgat ggccgcggtt tttcactggg aggagatttt tatgaggact
      121 gcaaaattcg ccctcggcct cttgtgtgcc gccactttga tgggcgcctc gcaagtcgaa
      181 gccaagcctg gtggctgcct gaagtacggc gtggcgggcg cagtcgccgg tcattatgca
      241 ggtcatcatg ccatgaaggg cgcgatcgcc ggctgtctcg ccgggatggc tcgccgcaaa
      301 gtgtataacg acgaattggc gcggcaaaag gcggacgcgg cgaagaaaaa cgccacgatc
      361 acgggcgcac ccgtcgaatc gcctccgcca acaaccagcg acgccccttc aacggccacg
      421 cctgcgactg atcagaagta agccgacgcc gatcgcgctt tcgggatcgt cccgtcgcgc
      481 agtttgcgct cccatgcata agcttggttc acgatctcgc gaagatcgtc gtattgtggt
      541 gtccagccaa gcttcgcccg aatgcgatcc gcgcccgcaa cgagcgcggc cggatcgcca
      601 gggcgccgcg gcgacagccg cgtttcaaag tcgacgccgg aaacgttttt aacgacatcg
      661 acaacttcgc gtaccgaatg accgtggccg tagccacaat tcagcacgag actctcgccg
      721 ccggcgcgca aatgtctaag cgccaacaga tgcgccgcga tgagatcagt gacgtggata
      781 taatcgcgca cgcaggtgcc atcggcagtc gcataatccg tgccgaaaat atcaaggccg
      841 gaacgcattc caagcgcagc ttgcactgca acctttatta gatgtgtcgc gttaggggtg
      901 gactggccaa gacggccttt cggatcggcg cctgcgacgt tgaagtagcg cagcgccaca
      961 taggaaagcc catgcgcgcg agcagcatct tcgagaatcc actcgaccat gagtttagat
     1021 ctaccatacg gattgatcgg cgccgccggc gcctcctcga aaatgggatt aatggcgggc
     1081 tcgccgtaaa cggcagcggt agaggaaaaa ataaattttc cgatgccgtt ccttacagcg
     1141 aattccagga gagctcgggc cttgaccgtg ttgttgagat aatagccgag tgggtcggag
     1201 actgattccg gcacgacgat cttcgccgca aaatgcgcaa tcgcatcgat atgatgtgct
     1261 gcagcgatgg cgcgaagctt agcttcgtcg ctgatgtctc cgacgacgag cgtcgccgcc
     1321 gaaggcacgg cccaggcaaa tcccgtcgaa agatcatcga gaacaacgac ctgctcgccg
     1381 gcatcgagca gcgccaaaac catgtggccg ccaatatagc ccgcccctcc ggtgaccaag
     1441 atcgccatgc cgcgctttct cccgctgtta agttttcgcc aattcgccaa aattgccaga
     1501 tagttgctcg cactataggc aagttgacgc tcagttgttc tgcattctaa gtaaaaacct
     1561 cagattgaat tttttcttag ctttcctgcc cccaaacgga ctttccaaca agtcggactt
     1621 ccaacggacg gactttcaat gagccaacgc gtacgcaaag ctgtcttccc cgtcgccggc
     1681 ctcggcacaa ggtttctgcc ggccacgaag tccgtcccca aagaaatgct gactgtcgtc
     1741 gaccggccga ttttgcagca tgtggttgaa gaggccttcg aagctggcat tgaacattgc
     1801 attttcgtga cgggtcggaa caaggcggtc atcgaagatt atttcgatat ggcttacgaa
     1861 cttgaagata ttttgctcaa gcgcggcaag accaaagaat ggcaggcttt gaaagccgat
     1921 ttgccgaagc ccggcgcgat gagctttacc cgccagcagg cgccgcttgg cctcggccat
     1981 gccgtctggt gcgcgcgcga cattgtcggc gacgaaccct ttgccgtggt tctgccggac
     2041 atgttgacca tgccaagaag gaccggtggc aagcgttgtc tggcgcagtg cgtcgaagcc
     2101 ttcgagacac atggcggcaa tgtcatcgcc gttgaagaag tcaaacccga agaaacccat
     2161 aaatacggcg tcgtctcggt tggcgacaaa tccggcagca cgttcgaaat cactggcatg
     2221 gtggaaaagc cgccgcaggg cactgcgcag tctaatttca tcatttcagg gcgttatgtt
     2281 ctctcgccgg aaatcttctc gatcctcgag cgcggtgaga aaggttcagg cggcgaaatc
     2341 caactgaccg acggcatgaa gtccctcgcg cacgatcaga aatttcacgg cgtcaacttc
     2401 gacggccaaa cctacgattg tggatcgagc ctcggcttcc tgggcgccaa tgttgccttc
     2461 gcgctcgcca gccccgatat cgcgccggaa ttccgcgcgc tggtgaaatc gctgttgaac
     2521 gcgcctcagg attaaagctg gaatttcaac ccggcgagaa aaacgtgatc gatgtagttg
     2581 gtgaagccgg ctgtgctggt gtagcgctga tatttatatg acccggtgat catgatggac
     2641 cgggtcatat gataatcggc ctcgaatagc ccggaataaa actggtcgat ctccggctcg
     2701 ccgatataat tgttgatctg atatgtaccc gtcgccgcaa gcgtgagatt gcgcagaaga
     2761 tcatgtgata tcttcagact caatgtatgc gcaacgaccg cagatgaatt ggcaagcgtc
     2821 gtttcgccaa cgtcggtcgc tgccgtgaag gtcaatgtcg tcagcggcgt cgcatcgtaa
     2881 atgagactgg cattgacagt cggtgcacgg gcaaccggca agcgcgggtc ggcatagaaa
     2941 cgttggtcat aaccgaccga gatgtcgccg gtgagaagcc tcgtcacttc gtaggttgta
     3001 ccgacctggg cgagaacgcc atttgaactc cggtcaaagc cgtcgacatc cattgacgag
     3061 tcgtattggt tccgcgtgcc cgtcacttgc acgtaaggaa tgagcgccgg cgtcaattca
     3121 taagagacgc ggccggcaac gctgtaggca ttgtaattgt tcaatgccag ttgaagatcc
     3181 gtaccgtcgg actgcgtcgc gttgccaaag aaataatgcg cgaaagcact gttgatgctg
     3241 accgaaagcc gattgaattg ctgcgtaaat ccgagcgttt gcccaatgct cgcataaagc
     3301 ggacggctgg tgacgaagac agcgttcggc accgaaagaa gcggtgatcc cggcgtcccc
     3361 tccgtcagac taaacgtggt ttgaaggttg atctgcgatt gccgcgtaac gtcaatgcgg
     3421 cccgtgatcg tgccggtagc attaggttga ttggcggtag ggtcggtgag gtaatcgtaa
     3481 tagccgccat gcaaatcggc cgcgaggctg ctctgcgacc agtccgattg cagcttgagg
     3541 cctgtttcgc cgtaaatata gggcgagcct ttgacatcgg tgttgagaag atttggattg
     3601 gaatcatacc cggtcgcggt ttcgacataa ggatatagac gtagcgatcc cacgccgaca
     3661 ccaatggcat cgaacggcgt gagatcggcc ttcggccgca agggttgcgg caagatcgga
     3721 atgacggcaa ccgtcggtcc cgggtcggcg gggagatcgg cgatgctacg ccgccgcaga
     3781 gcgggcgccg ccgccgtcgc ataggctctc aacagcggta gcggtctctg cccgacgcgc
     3841 ctgatcttcg gcaattgata gagcgccgat tttttcggcc gcgggcggcc gtagttggtg
     3901 acgcggcccg atgccgtgac cggtgcgggc aggccgtaat tcgccggggg cgcggcgact
     3961 ggattgagat catccgagtc cggcgaaatc gtcgccccag ggagatcatt gagggctggc
     4021 ccttccgagc cgcgcaattc cggcggcggc agcggcgggg cttgcataac gggtgcctgc
     4081 gcatcgtcgc tggagggtgc cggtggctcg ggcgtcatcc tctgtgccca agcgcaacca
     4141 tgcgcggcga cgacaagaag cgcggccgcg gctgctttga ggcagagtct ggctgaaacc
     4201 ggcacgcacc ctcgctgtcg cagcaggaat tcgacgagtt tgggcgggat cgacgcgaaa
     4261 aaccgcgaac actcttccgc atcccgcccg gaaggctgcg accaatgagg gttaggggaa
     4321 catggttaac gccgtcttag cggcactgca aaaacgtctc gaaacgggac gggcgggcga
     4381 cgctctttcg atcgccgtga tgccccactg accggcgaac ggaactcgac cctggctatt
     4441 gcgttaaagg gacggcccat tctgacgtcc ggtacaaagc catgaccctg cttctgataa
     4501 ttgtcgtcct gatccttctg ttcggcgggg gcggcggcta ttatggctat aaacgcggct
     4561 attatggcgg caatggcttg ggctgc
//
LOCUS       DASWOG010000467         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_47342,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000467 DASWOG010000000
VERSION     DASWOG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47342"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..365)
                     /locus_tag="VGB93_09635"
     CDS             complement(168..365)
                     /locus_tag="VGB93_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146391.1"
                     /translation="MGELLAFRAQTRVARSAAPRGEEAEILFFTGVRYIRTETYHEAA
                     IRSDRMDTKRKSVAQREQAPH"
     gene            complement(521..862)
                     /locus_tag="VGB93_09640"
     CDS             complement(521..862)
                     /locus_tag="VGB93_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY0146392.1"
                     /translation="MRLRSDIWIAAYLRRCGVEGAPAYLRRRGAAEAGTIFVKIDRLD
                     GQAALFGPAPPSEVDDGVRLFTRLHKEEWLDPGAIEARLKREIDFDVDLWIVEVEDRL
                     GRNFLDLADAS"
     gene            1035..>1921
                     /locus_tag="VGB93_09645"
     CDS             1035..>1921
                     /locus_tag="VGB93_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0146393.1"
                     /translation="MKAVIVFLTIIGCGLLFAAFSVHNDVEATGTVVKSALPYLLLGV
                     ALVIALGFEFVNGFHDTANAVATVIYTRSLPAQVAVVWSGTFNFLGVLTSTGAVAFGI
                     VSLLPVELILQVGSEAGFAMVFAMLIAAIIWNLGTWWLGLPASSSHTLIGSIIGVGVA
                     NALMRGRDGTSGVDWGKATEIGYALLLSPLVGFIFSALLLLTFKALIKNPDLFKEPKG
                     DQPPPAWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRAVPEHHV
                     QTFISTSEAASKVIDAKASG"
BASE COUNT          355 a          611 c          537 g          418 t
ORIGIN      
        1 gacgacgatg aaaatgatca gccgttgaag gaattgcgcc tcgtcgcccc ggacgccaag
       61 ggcttcgtcc tgatcaacgc tctttcggcc tatccgagcg aacgcgctga ggccgaggcg
      121 gaagtcaaat cggtcgcctg cgcccccgaa acaattgcgc aagacaatta atgcggcgcc
      181 tgctcgcgct gggcgacact tttgcgcttt gtgtccatgc gatcggatcg aatggcggcc
      241 tcgtgataag tctctgtgcg aatgtaacgc acacccgtaa agaagagaat ttccgcctcc
      301 tcgccgcgcg gcgctgcgct gcgcgcaacg cgcgtttgcg ccctaaaagc taacagctcg
      361 cccatggttt gacctccacg cggccctgcg tccgtcccta ttctccgcat aacgccgagc
      421 ccgtgaggac aagcgtcacc ccgggttggt taatgctttc tcaacggatt ttaaaagatt
      481 ggaaagaaaa gcgcggcggg gctccggcgc tacaaaggca tcaggacgcg tctgcgagat
      541 cgagaaaatt gcggccgagg cgatcttcga cttcgacgat ccaaagatcg acatcgaagt
      601 cgatttcgcg tttaagccgc gcttcaatgg cgccgggatc gagccattct tctttatgaa
      661 gccgggtaaa aaggcgcacg ccgtcatcaa cttcgctcgg cggtgctggg ccaaacagag
      721 cggcctgtcc atcaagcctg tcgatcttta cgaatatggt gccggcttcc gccgcgcccc
      781 gtcggcgtaa gtaggcgggt gcgccttcta cgccgcagcg ccgcaaatac gcggcaatcc
      841 agatatcgct tcgcaatcgc attaaactgg acccatatcg cccgcatccc caactttgtc
      901 acaaaactgc aatgcacttc ggtttggtta gcgccgccgt ttggaggatg cgcgccatga
      961 cttccctttc atctattgat accgccccgc aagagacgcg aggtcctgcc ccgaaactcg
     1021 atcatccgat cgatatgaag gctgtgattg tttttcttac gattattggc tgcggactgc
     1081 tgttcgccgc tttcagtgtg cataacgacg tcgaagcgac cgggacggtc gtcaaaagtg
     1141 ccctgcctta cctcctcctt ggcgtcgctt tggtgatcgc ccttgggttc gaattcgtca
     1201 acggcttcca tgataccgcc aacgccgtcg ccactgtcat ctatacccgc tcgctgccgg
     1261 cgcaggtcgc cgtggtgtgg tcgggcacct tcaacttcct tggcgttcta acctcgaccg
     1321 gtgccgttgc cttcggcatc gtctcgttgc tgccagtgga gttgatcctc caggtcggct
     1381 ccgaagcggg tttcgccatg gtgttcgcca tgttgatcgc cgcgatcatc tggaacctcg
     1441 gcacctggtg gctcggcctc ccggcgtcga gctcccacac gttgatcggc tcgatcattg
     1501 gcgtcggcgt tgctaacgca cttatgcgtg gccgtgacgg cacctcgggc gtggattggg
     1561 gcaaagccac ggagatcggc tatgcgctgc tcctgtcgcc gctggttggc ttcatcttct
     1621 cagcgcttct gctgctgacc ttcaaggccc tcatcaaaaa ccccgatctc ttcaaggagc
     1681 cgaagggcga tcaaccgccg ccggcctgga ttcgcggtct gctggtcctg acctgcaccg
     1741 gcgtttcctt cgcccacggc tcgaacgacg gtcagaaggg catgggcctt atcatgctca
     1801 tcctgatcgg caccgtgccg acggcctacg ccctcaaccg cgcggtgcct gagcaccacg
     1861 tccagacctt catctcgacc tcggaagctg cgtcgaaagt catcgacgcc aaggcgagcg
     1921 g
//
LOCUS       DASWOG010000468         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_323192,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000468 DASWOG010000000
VERSION     DASWOG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323192"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="dnaX"
                     /locus_tag="VGB93_09650"
     CDS             complement(<1..873)
                     /gene="dnaX"
                     /locus_tag="VGB93_09650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0146394.1"
                     /translation="MAEDHSTEPSLLPAGGDAPPAAPYRVLARKYRPTNFDDLIGQEA
                     MVTTLSNAFDLSRIHQAYILTGVRGVGKTTTARILARAFNYEVPAQDGKAAVSQPTIR
                     MPELGVHCQAIIDSRHVDVIEMDAASHTGIDDIREIIDNARYRPVLARTKVYIIDEVH
                     MLSKAAFNGLLKTLEEPPDHVKFIFATTEIEKVPVTVRSRCQRFDLRRIDADRLVAHL
                     ATICTKEELATGPEALALIARAAEGSVRDALSLLDQAIAHAKGSLRSEVKAVDLRQML
                     GLADRTRLIDLFADV"
     gene            complement(971..1068)
                     /gene="ffs"
                     /locus_tag="VGB93_09655"
     ncRNA           complement(971..1068)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGB93_09655"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1162..1704
                     /locus_tag="VGB93_09660"
     CDS             1162..1704
                     /locus_tag="VGB93_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146395.1"
                     /translation="MRCLSKAEAAAILDPRGYSIRPPAPIDRLGARPPADIAVLPDFI
                     RELSRWLSPDGERLLWIDHWEPGAYGFDHAILALIRAGLGEPRALDEAPGYLFEAQNW
                     AAEDQAEVSIEMARNLGELVGLTSLVMLTQSYGWLLSPESADRIEFWEGNFFFYSREP
                     DQIIRAYTIVDQFDCSRDLK"
     gene            1719..2420
                     /gene="queC"
                     /locus_tag="VGB93_09665"
     CDS             1719..2420
                     /gene="queC"
                     /locus_tag="VGB93_09665"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY0146396.1"
                     /translation="MTKALVLFSGGQDSTTCLAWALARFDHVETIGFDYGQRHRIELD
                     QRAKIREKIVALRPGWAGRLGEDHLIDLAVLGQISATAMTRDVEIVMQENGLPNTFVP
                     GRNLVFLTFAAAVAYRRGLTHIVGGMCETDFSGYPDCRDATIKAMQTALNLGMEKDFV
                     LETPLMWLDKAQTWALAEELGGKPLVDLVLEDTHTCYLGERGVRHEWGYGCGTCPACT
                     LRAQGWAKYRAAAAA"
     gene            complement(2430..3938)
                     /locus_tag="VGB93_09670"
     CDS             complement(2430..3938)
                     /locus_tag="VGB93_09670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEY0146397.1"
                     /translation="MPYRFSSFRRLAGIVAAVALAALLSGCVGEEDSGSDGLVDRAYA
                     PLSPQMLALMQAKGTSPQAPVLFRTYKKEAEFEVWKMRSDGRYTLLRTYPMCRWSGQL
                     GPKKKQGDRQVPEGFYAIYPSQMNPHSNYYLSFNVGYPNLYDRVHGYSGDSIMVHGIC
                     SSAGCFSMTNPEIGEIYTIAREAFAAGQPEIQMQSMPFHMTAENLAKYRLDPNVDFWR
                     ELKNGADNFEVTKHEVAVGVCNMHYVFNAKPANGESFDPAAPCPVLRRDEMVHDEVVA
                     REARDDARIAELVGTGVAAVHTAYADGGQNPVFKGAKIDDVSRPDALATGPVDVAIKR
                     HGRAPSLIQLKAQRAKDLAAAIAAEKKAQALRIARGETDPVDTQPTGSTGAPAVAAPA
                     PQKPAPFSNFFGFNKSAQPAATPAPAVATAEPPQSQPGAPAPQQPAQTVPTPSAAPSA
                     SHGFSLTNMFAPKPAQQSAPAAQAASATPSAVQSAAPADTQSPAPVTPTNGQ"
     gene            complement(4020..4376)
                     /locus_tag="VGB93_09675"
     CDS             complement(4020..4376)
                     /locus_tag="VGB93_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146398.1"
                     /translation="MAFMVNRKIIRGLRLGLASIGLVLAGLLAMTVARAADLDHPYYR
                     HRRHLPPPTPVAFLPPPPSCKIVPLPEMNLYNEVTRAGIQNVCISRGVYADSFWPYAP
                     PYWRPNPHQGYAFGFW"
     gene            complement(4466..4840)
                     /locus_tag="VGB93_09680"
                     /pseudo
     CDS             complement(4466..4840)
                     /locus_tag="VGB93_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408677.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
BASE COUNT          851 a         1458 c         1553 g          978 t
ORIGIN      
        1 gacatcggca aagagatcga tgagccgcgt ccgatcggcg aggccgagca tctgccgcag
       61 atctacggcc ttcacctcgc tccgcagact gcctttggca tgggcaatcg cctggtcaag
      121 cagagagagc gcgtcacgca cggagccttc cgccgcacgg gcgatgagcg ccaaggcctc
      181 aggcccggtg gcgagttctt ctttcgtgca aatggtcgcg agatgcgcga cgagccggtc
      241 ggcgtcaatg cggcgcagat cgaaacgctg acagcgcgag cgcaccgtca ccggcacttt
      301 ctcgatctcc gtcgtcgcga agatgaactt cacatggtcc ggcggctctt cgagcgtctt
      361 caacaggccg ttgaatgcgg ccttcgagag catgtgaact tcgtcgatga tatagacttt
      421 ggttcgcgcc aggacgggcc ggtaacgggc gttgtcgatg atctcgcgga tgtcgtcgat
      481 gccggtgtga gaggcggcgt ccatctcgat cacatcgaca tgacgcgaat cgatgatcgc
      541 ctggcaatga acgccaagct ccggcatccg aatggtcggc tggctgacgg ccgccttgcc
      601 gtcttgcgcc ggcacttcgt aattgaaagc gcgggcgagg atgcgcgcgg tcgtcgtttt
      661 gccgacaccg cgcacgccgg tcagaatata ggcctgatgg atgcggctga ggtcgaacgc
      721 gttcgacagc gtcgtgacca tcgcctcctg accgatgaga tcgtcgaaat tggtcggccg
      781 gtatttgcgc gcgagcacgc gatacggcgc ggccggaggc gcatcgcccc cagccggcag
      841 aagggaaggt tcggtgctgt gatcttcggc cattcagccc gtcatcgcga gcggagcgaa
      901 gcgatccggt cttgtcccgg cttgcttggc cgctgcgttt ctcgcaacga cgcagcgctc
      961 gaaaataaga agtgggaggc tggacagaaa cccgcccggt tctcgtctgg gctgcttcct
     1021 tccggacctg acccggttgg cgagtggtgc gtccaccgcc aacctcccgg ctcttatttg
     1081 gactatcgaa gccaaatccg caatagattg tccagcgaca atggccgaag gtggccgaac
     1141 ccggaatagg gcgttttttc aatgcgttgt ctgtccaagg cggaagccgc cgcgattctc
     1201 gacccgcgcg gctattcgat tcggccgccg gcgccgatcg atcgtcttgg cgcacggccg
     1261 cccgcagaca ttgccgttct ccccgatttt atccgcgaat tgagccgctg gctgtccccg
     1321 gatggcgaaa ggctgctttg gatcgatcat tgggagcccg gcgcttacgg attcgaccat
     1381 gcgattcttg cgttgatacg tgcgggattg ggcgagcccc gcgctttgga cgaggcaccc
     1441 ggatatttgt tcgaggccca gaattgggcc gctgaggatc aggccgaggt ttcaatcgag
     1501 atggcgcgaa atctgggtga gcttgtcggg ctgacctccc ttgtcatgct gacccaatct
     1561 tatggctggt tgctatcgcc ggagtccgcc gatcggattg agttttggga aggcaatttc
     1621 tttttttatt cgcgcgaacc cgaccagatc atccgcgcct acacgatcgt cgatcaattc
     1681 gactgttcgc gcgatctgaa ataaaaaagg cgatcatcat gacgaaagcg ctcgtgcttt
     1741 tttccggagg gcaggattcg acgacctgtc tcgcctgggc gcttgcccgc ttcgatcatg
     1801 tcgaaacgat cggtttcgat tacggccagc gccaccgcat cgagctcgat cagcgggcca
     1861 agatccgcga gaagatcgtg gctctccgcc caggctgggc cggacggctt ggtgaggatc
     1921 atctgatcga tcttgccgtg cttggccaga tttcagcgac ggcgatgaca cgtgatgtcg
     1981 agatcgtcat gcaggagaat ggcctgccga ataccttcgt ccccggtcgt aacctcgtct
     2041 ttctgacctt cgcggcggcg gtcgcctatc gccgcggcct cacgcatatc gtcggcggca
     2101 tgtgcgagac ggatttttcc ggctatcccg attgccgcga cgcgacgatc aaggcgatgc
     2161 agacggcgct caatctcggc atggaaaagg acttcgtgct cgagacgccg ctgatgtggc
     2221 tcgacaaggc gcagacctgg gcacttgccg aggagcttgg cggcaagccg ctcgtcgatc
     2281 tcgttctcga agatacgcac acctgctacc tcggcgagcg cggcgtgcgg cacgaatggg
     2341 gctacggctg tggcacgtgt ccggcttgca cattgcgtgc gcaaggctgg gcgaagtacc
     2401 gcgccgcggc cgcggcttaa gcctaaaaat tattggccgt tcgttggtgt cacgggtgca
     2461 ggagactgcg tatcggcagg cgctgcactt tgcacagcag atggcgtagc gctcgcggcc
     2521 tgcgcggccg gggccgactg ctgcgccggt ttcggcgcga acatgttggt gagggaaaag
     2581 ccgtggcttg ctgacggcgc cgcagatggc gttggtacag tctgtgccgg ctgctgtggg
     2641 gcgggtgcgc ccggttggct ttgcggcggt tcagccgtcg cgacggccgg agctggggtc
     2701 gccgcgggtt gcgccgactt gttgaagccg aagaagttcg agaatggcgc gggcttttgc
     2761 ggcgccgggg cggcgaccgc tggtgcaccg gtgctccctg tcggctgcgt gtcaaccgga
     2821 tcggtttcgc cgcgcgcaat acgcaacgcc tgcgcctttt tctccgcggc gatcgccgcc
     2881 gccaggtctt tcgcccgttg ggctttcaat tgtatcagcg acggcgctct gccatgccgc
     2941 ttgatcgcga catccaccgg gccagtcgca agcgcgtccg ggcgactgac atcgtcgatc
     3001 ttcgcgccct tgaagacggg gttctggccg ccatcggcat aggcggtgtg aaccgctgcg
     3061 acgccggtgc cgacaagctc ggcgatgcgc gcatcgtcgc gcgcttccct ggcgacgact
     3121 tcgtcatgca ccatctcgtc gcgtcgcaag acagggcagg gggccgctgg atcgaaactt
     3181 tcaccattcg ccggctttgc gttgaacacg taatgcatat tgcagacgcc gaccgcgact
     3241 tcgtgtttgg tcacctcgaa attgtcggcg ccgttcttca gctcgcgcca gaagtcgacg
     3301 ttggggtcga gccggtattt cgcgagattc tcggccgtca tgtggaacgg catggactgc
     3361 atctggatct ctggctggcc ggcggcgaag gcctcgcgcg caatcgtgta gatttcgccg
     3421 atctccggat tggtcatcga gaaacagcca gccgacgagc agatgccgtg caccatgatc
     3481 gaatcgccgc tgtagccgtg gacccgatca tagaggttcg gatagccgac gttgaacgac
     3541 aggtagtagt tcgaatgcgg attcatctgc gacggataga tcgcgtagaa cccttccggc
     3601 acctgccggt cgccctgctt tttcttgggg ccgagctggc cggaccagcg gcacatcgga
     3661 taggttcgga gcagcgtata acgcccgtcc gaacgcatct tccagacttc gaattccgct
     3721 tccttcttgt aggttcgaaa gaggaccggc gcctgcgggc tcgtgccctt ggcctgcatc
     3781 agggcgagca tttgcggcga caaaggcgcg taggcgcgat caacgagacc gtcgcttcca
     3841 gaatcctctt cgccgacgca gccgctcaga agtgcggcca gagccacggc cgcaacaatc
     3901 ccggcaaggc gtcgaaagga cgaaaaacgg tacggcatcg gcataattct tgacccaggc
     3961 cccctgacct cgccgccatc taagggcgtt atccttattg gtcggttact tcaatctggc
     4021 taccaaaagc cgaaagcgta gccttgatgg ggatttggcc gccaataagg cggcgcgtag
     4081 ggccagaagc tgtcggcata cacgccgcgc gaaatgcaga cattctggat gccggcccgt
     4141 gtcacctcat tatagaggtt catctccggc agcggcacga tcttgcagct cggtggcggc
     4201 ggcagaaagg cgaccggagt cggcgggggc aggtgcctgc ggtggcgata ataggggtga
     4261 tcgaggtccg cggcccgggc aaccgtcatc gccaaaagcc cggccagaac gaggccaatc
     4321 gaggcgaggc caagccggag cccgcggatt atctttctgt taaccataaa agccattcgt
     4381 ccggctccgg cgcggccgat cccgcacgat tcgccggaca atggcgcctt aagcttgcct
     4441 ctcgcttaag tcgcgcgcct tctcctcaag cctttggctt ggcgaagaca gactcggcgt
     4501 cgaaatggtc ttcctcttgc ggcgtcgccg gcctgtgcga gaagacatcg ccatcatgct
     4561 ggccttcggg gcgtggcgcg gtcgtgatct cggccggtac gagcgtctgc ttgcggtcct
     4621 cttcgaccgg cacccgttcc ttggccgcgc gattgacctc gaaatcgaga tccatctgcg
     4681 agcaaaggcc gagggtgaca gggtccatcg gcgccagggc cgccgaattt cagtgcgtgc
     4741 ggttacggat cgcctgcagc gtcgttttcg tcgtgccgac gagccgcatg atctgcgcat
     4801 ccttcaattc cggatggttg cgcaagagcc agaggatcgc
//
LOCUS       DASWOG010000469         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_47381,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000469 DASWOG010000000
VERSION     DASWOG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47381"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="VGB93_09685"
     CDS             <1..2381
                     /locus_tag="VGB93_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0146399.1"
                     /translation="DPYAETVSQQIDLLVNYTAGDGFTYRQSARQQVAVATELALVGT
                     IAALVLSGLVVLLLSRRITKPVAAASAVAKRIASGELEGEIPAGSGDELGALLAAMRV
                     MRDNIRAMMEREVAQRRTAQVRLADALESSREGIVVVDADHRIALANSQAKEFFGSAM
                     GLLEPGKSAGAATQSNDLALATLSLEGLSPGTEEFRLPDGRWLRVSRSATEDEGFITL
                     YSDITVLKEQEAKLKATNLLLDAALDNMSQGLCLYDSEQRLKVVNRRFCEIFRLSPAQ
                     LRPGIVFRDVLALSIAANNHGDETADDLYAQEIPLISGRISATRFLELSQNRVILIAR
                     QPTADGGWVATYEDATERRRAEERIVFMARHDALTGLPNRLLFAERIEAAISQIGRVH
                     SGFAVLSLDLDHFKQVNDTLGHPVGDALLRSVAERLQACVREVDTVARLGGDEFAILQ
                     PGLDKPEDAAVLGRRIVEVLSAPYEIEGNRLTISVSIGIAIAPGDGMAYDKLLKSADV
                     ALYLAKADGRAIWRFFEPEMDVRLQVRRALELDLRDALVNNEFQVFYQPIYDLRENKI
                     GGFEALLRWNHPTRGLVAPTEFISLAEEIGLIVPLGEWVLATACAEAATWPKYIKLAV
                     NVSVVQFKNELFVPMVTDNVRAASLSPRRLELEITESLLLNNTAATLAKLHQLRDFGV
                     HIAMDDFGTGYSSLSYLRSFPFDKIKIDQSFIRTMSDKDGSRAIVRAITAMGGSLGIR
                     TTAEGVETHEQLAWLRATGCDEAQGYLFSRPVPASKIPRLLMEWNGLKREAV"
     gene            2522..>2925
                     /locus_tag="VGB93_09690"
     CDS             2522..>2925
                     /locus_tag="VGB93_09690"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0146400.1"
                     /translation="MMRPLAILPSVLAADWLYMGDSVQKVMAAGADIVHLDVMDGHFV
                     PNISFGPDMVKAIRSITDARLDVHLMIAPADPYIEAFAKAGADVITIHPEVGPHLHRS
                     LGAIRQLGKKAGVALNPATPISAIEHVLDQLDL"
BASE COUNT          562 a          945 c          860 g          558 t
ORIGIN      
        1 ttgaccctta cgccgagacg gtgtcgcagc aaatcgacct gctcgtcaat tacacagccg
       61 gcgatggctt tacctaccgt caaagcgcgc gccagcaggt tgccgtcgcg acggaactcg
      121 ctctggtcgg gacgatcgcc gcgcttgtct tgtcgggtct tgtggtgctg cttttgtcgc
      181 ggcggatcac caagccggtc gccgcggcgt ccgccgtcgc caaacggatc gccagcggtg
      241 agctcgaagg cgaaattccc gccggtagcg gcgatgaact cggtgcgttg ctcgccgcca
      301 tgcgcgtcat gcgcgacaac atccgagcca tgatggagcg cgaggtcgcg caacgccgga
      361 ccgcccaggt ccggctcgcc gatgcgcttg aaagctcgcg cgaaggtatt gtcgtcgtcg
      421 atgccgacca ccgtatcgcg ctcgccaatt cccaggccaa ggaatttttc ggtagcgcaa
      481 tgggactgct ggagccaggt aaatctgctg gcgcggccac gcaatccaac gatctcgccc
      541 tcgccacgct ctcgctcgaa ggcctctcgc cgggcaccga agaattccgc ttgccggacg
      601 ggcgctggct gcgcgtcagc cgcagcgcga ctgaggacga aggtttcatc accctctaca
      661 gcgacatcac tgtcctgaaa gagcaagagg caaagctcaa agcgacaaat ctgcttctcg
      721 acgctgcgct cgacaacatg tctcagggtc tatgcctcta cgacagcgag cagcggctga
      781 aggtcgtcaa tcgccgcttc tgcgaaatct tccgcctttc gccagcgcaa ctgcgtccgg
      841 gtattgtttt ccgcgatgtc ctcgccctca gcatcgcagc caacaaccac ggcgacgaga
      901 ccgcggatga tctttacgcg caagaaatcc cactgatcag cggccgtatc tcggcaacgc
      961 gttttctcga gttgagccag aatcgagtca ttcttatcgc gcgtcagcca acggctgatg
     1021 gcggctgggt ggcaacctat gaagacgcga ccgagcgccg ccgcgccgaa gagcgcattg
     1081 tcttcatggc acggcacgac gcgctgaccg gattgccgaa ccgcttgctc ttcgccgaac
     1141 ggatcgaagc cgcgatcagc cagatcgggc gtgtccatag cggcttcgcg gtgctgagcc
     1201 tcgatctcga tcacttcaag caggtcaatg acacgcttgg ccatcctgtt ggcgacgcgc
     1261 tgctgcgttc ggtggcggag cggctgcaag cctgcgtgcg cgaagtcgat acggtcgctc
     1321 gtctcggcgg cgacgaattc gcaattttgc aacccggcct cgacaagccg gaagatgcgg
     1381 cggtcctcgg ccgccgcatt gtcgaagttc tcagcgcgcc ttatgagatc gaaggcaacc
     1441 gtctcacgat cagcgtcagc atagggatcg cgatcgcgcc cggcgacggc atggcatatg
     1501 acaagttgct gaaaagcgcc gatgtcgcgc tttacctcgc caaggcagat ggccgggcga
     1561 tctggcgctt cttcgaaccc gagatggatg tccgactaca ggtgcgccgc gcactggaac
     1621 tcgatttgcg ggatgcgctg gtaaacaacg aattccaggt cttctaccaa ccgatctacg
     1681 atttgcgtga aaacaagatc ggcggtttcg aggccctgct gcgctggaac catccgacgc
     1741 gcggtctcgt cgcgcccaca gaattcattt cgctcgccga ggaaatcggg cttattgtgc
     1801 cgctcggcga atgggttctg gcgaccgcct gcgccgaggc cgccacctgg ccaaaatata
     1861 tcaagctcgc ggtcaacgtt tcggtcgttc agttcaagaa cgagcttttc gtgccgatgg
     1921 ttacagacaa cgtacgcgcg gcaagccttt cgccgcgtcg gcttgaactc gagatcaccg
     1981 agtcgctgct gctcaacaac acggccgcga cgctcgccaa gctgcatcaa ttgcgcgatt
     2041 tcggcgttca catcgcaatg gatgacttcg gcaccggcta ttcgtcgctg agctatctgc
     2101 gcagcttccc cttcgacaag atcaagatcg accaatcttt catccgcacc atgtcggata
     2161 aggacggttc gcgcgctatc gtccgcgcca tcacggcgat gggcggcagc cttggcatcc
     2221 gcacgacggc ggaaggcgtc gagacgcacg aacaactcgc ctggctgcgg gcgacgggct
     2281 gcgacgaggc acagggctat ctgttcagcc gcccggtgcc ggcaagcaaa atcccccgcc
     2341 tgctcatgga atggaacggc ctaaaacgcg aagcggtcta acctgcttgt ccacctctcg
     2401 acgcgcgcgc gccgcgcgtg ctatggcgtg gcatccggca cccgcagagc cgccagagtc
     2461 agaaaagcca atcttttcag agatctccgg ctaaatgcgt cgttcgcgcc ggtgaagagg
     2521 cttgatgcgt ccgcttgcca ttctgccatc cgttctcgcc gccgactggc tctacatggg
     2581 cgactcggtc caaaaggtga tggccgccgg cgccgacatc gtgcacctcg acgtgatgga
     2641 cgggcatttc gtccccaaca tcagtttcgg accggacatg gtcaaagcga tccgtagcat
     2701 cacggacgcg cggctcgatg tgcatctgat gatcgcccct gccgatccct atatcgaggc
     2761 cttcgccaag gcgggcgccg atgtcatcac aatccacccg gaggtcgggc cgcatttgca
     2821 tcgctcgctt ggcgccattc gccagctcgg caaaaaagcc ggggtggcgc tgaaccctgc
     2881 gacccccatt tccgcgatcg aacatgtgct cgatcaactc gacct
//
LOCUS       DASWOG010000470         7168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_84048,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000470 DASWOG010000000
VERSION     DASWOG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84048"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(757..3210)
                     /gene="gyrB"
                     /locus_tag="VGB93_09695"
                     /pseudo
     CDS             complement(757..3210)
                     /gene="gyrB"
                     /locus_tag="VGB93_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610463.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
     gene            complement(3537..6155)
                     /gene="clpB"
                     /locus_tag="VGB93_09700"
     CDS             complement(3537..6155)
                     /gene="clpB"
                     /locus_tag="VGB93_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0146401.1"
                     /translation="MDFEKYTERARGFVQSAQSLATREGNQQFMPEHLLKVLLDDPEG
                     LASGLINKAGGNAKEALTQTELALAKLPKVQGSGAGQLYIAPATARLFDNAEQIAKKA
                     GDSYVTVERLLLALALEKGTEAAKILQSAGVTPQVLNAAIEDLRKGRTADNATAENAY
                     DALKKYARDLTEAARAGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRIVNGDVPESLRDKSLLALDLGSLIAGAKYRGEFEERLKAILNEVTSAQGGII
                     LFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCIGATTLDEYRKHVEKDAALARR
                     FQPVFVNEPNVEDTISILRGLKEKYELHHGVRITDSAIVAAATLSNRYIADRFLPDKA
                     IDLVDEAASRLRMQVDSKPEELDEIDRRVVQLKIEQEALKKETDTASKDRLQRLEHEL
                     AEAEEKSAALTSRWRAEKDKLDSAQKIKEQLEAARTELLQAQRRGEYQRAGELTYGVI
                     PDLEKKLADVESKGSEVLVDEAVTADMVAGVVSRWTGVPVDKMLEGERGKLLRMEDEL
                     ARRVVGQAEAVAAVATAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAEFLFD
                     DETALIRVDMSEYMEKHSVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEIEKA
                     HPDVFNVLLQVLDDGRLTDGQGRTVDFRNVLIIMTSNLGAELLVMQPEGQDTAAIRDD
                     VMQIVRAHFRPEFLNRIDEIIMFHRLRRQDMAAIVDIQMGRLSRLLDDRKIILEMAET
                     AREWLADKGYDPAYGARPLKRVIQKALQDPLAEKILAGKIHDGEVVKVGGGPEGLIFG
                     NVEPPKSGGKGNAKVA"
     gene            complement(6330..>7168)
                     /locus_tag="VGB93_09705"
     CDS             complement(6330..>7168)
                     /locus_tag="VGB93_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY0146402.1"
                     /translation="AHHIGEKYLQLARDAQSSGDPVMAESYLQHAEHYFRIIATAQQA
                     QQAQFAGYNRPGTETDNEELEDEDDFSGLPDRFASPVAPSVTPPPQQPQPYVAQPTVP
                     NGQGQPNYSAQPTYQERPQYEPRGERGPRNFQDRNQDRNQDRNNQDRNQERPAYGERN
                     GQNGPRDNREGGNGRPRQPYRGDGAPRYDNAPRYERHEPRNVSEEATAAALPAFITAP
                     VPVRVQPEPELPVNDESVGTEVLARPEAEAEENNFALRPRRRRRVKPEFGVAETKASL
                     EE"
BASE COUNT         1286 a         2260 c         2104 g         1518 t
ORIGIN      
        1 agctgtgtct ccaagaatcc atgcccaagt gcatggtggt tcgcaggaga gatcggcgcg
       61 gggacacgtt cgtgacggtt agaacgatag tcacgcaatc caccattgcg cgtggggaac
      121 atatatttca ttttggattt cgatagtcgc cgccaatagg cgccttaaaa caagtttaga
      181 acaccgaacg gccgtcggat ctgatccggc tcgataatca gcaggtcccc aagatttcga
      241 atcgataaga tatagtgccc cgctaacgcg agttcggaag ctttgaaccg ctcggcgcaa
      301 gctcttcgca tggtcgattc cgtcgccatg ggtgatccac atttcatcgg cgtcgagtat
      361 ggcgaccaac ccttcgtcgt ttctttcggt cgaggccctt caccccaatc caccgcgcta
      421 tattgtccgc atattgaaca agccgcggat caaaacatcc taaactattg aaatcatttt
      481 gacgcccata gggaatcgaa ccccctgttt ttgccgtgca agggcaaaaa tagatcggag
      541 attttcactt tttaattgtg gcatttctca aatatcgatc gtctattcac cagttgcccc
      601 catccagacc cgccgtggaa tttgaccctc cggtgaagtt tctccctctt taggagactg
      661 cagagtcgaa cttcattgtt gatcgagtcg gactacggcc tcccagagtc gaagttttgc
      721 gttacgcgac atatgtgtta cgcgacactt ggggtcttaa acgtcgagat tggcgacgtt
      781 cagcgcgttt tcctggatga actcgcggcg cggttcgacg acatcgccca tcagcttgac
      841 gaaaatatcg tccgcttcgt cgccttcctt gatcttcacc tgcagcagcg agcgcgcatt
      901 gcggtcgagc gtcgtctccc acaattgctc ggcgttcatt tcacccaggc ctttgtaacg
      961 ctggatgtgt gaaatgccct tctgccccgc cgccatgatg agatcgacca attgcgacgg
     1021 gccgccaact tgcttctctt cgccgcggcg ggtcagtttg gcagggcggc tataaatatc
     1081 gcgcaggctc tgcgcatgtt cgttcagccg ccgcgcctcg cccgaggcca acaatgcgcc
     1141 gtcaagcatc gaggcgtggc gcacgccgcg caattcgcgc gagagaacgt aagtgccgtt
     1201 ttccacgcca ccttgccacc cccgctccat ctcgtcagca aaacgatcga gccgttcggc
     1261 aatttcgcgc gcatgatcca acgccgccgg atcatcgtca gaggcgatgg cctgcaacgc
     1321 cccggcaatc gccgcctgct cgacgatact gcggtcgtag cgcgaatgca ggcccgtgag
     1381 cacctgccgc acgccgcgcg cctcgtcaac gacagcacgc aaatcctgcc ccgcgcgtta
     1441 ctcgccattg tcgagcctca gcaccgcgcc gtcgagccca ttgtcgatga ggtaatcctc
     1501 gcgcgcgcgc tcgtccttca gatattgttc ggaaccgctg cgcttgacct tatagagcgg
     1561 cggctgggct atgaagatat ggccgcgctc gatcaattct cgcatctggc ggaaaaagaa
     1621 agtcagcagc agcgtgcgga tatgcgagcc atcgacatct gcgtcggtca tgatgatgat
     1681 cttgtggtaa cgcagcttgt cgggattaaa atcctcgcgt ccgatgccgg tgccaagcgc
     1741 cgtgatcaga gtgccgatct cttggcttga cagcatcttg tcgaagcgcg cccgctcgac
     1801 attgaggatc ttaccgcgca agggcagaac cgcctgaaac gcgcggtcgc ggccctgttt
     1861 cgccgagccg ccggcggaat cgccctcgac cagaaagagc tccgacttcg ccggatcgcg
     1921 ttcctgacaa tcggcaagtt tgccgggcag atttgcgaca tcgagcgcgc ctttgcgacg
     1981 cgtgagttcg cgcgccttgc gcgccgcttc gcgtgctagc cccgcctcaa tcaccttgct
     2041 ggcgacaatc ttggcgtcct gtggatgctc ctcgaaccac gagctaagca tttcgttgac
     2101 gataccttcg accgccggtc gcacttccga agaaacgagc ttgtccttcg tctgtgacga
     2161 gaatttcggg tccggcactt tgaccgagag cacgcaggtc aggccctccc ggcaatcgtc
     2221 gccggtgaga tcgaccttct cgcgtttggt aaggcccgag cgttcggcat agcccgtcac
     2281 ctgacgcgtc agcgccccgc gcagacccgc gagatgcgtg ccgccatcgc gctgcgggat
     2341 gttgttggta aaggccagca cgttctcgtg gtaggaatcg ttccaccaca aggcgacttc
     2401 aaccgtcatc cggtcgcggt cgcccttgat cagaatgggc ttgccgatca gcggcgattt
     2461 tgcgcggtca aggtagcgca cgaaggcttc aaggccacct tcctaataaa gctcctcgtg
     2521 cctggtctcc gcagtgcgca catcggtcaa ttcgattcgc acgccggaat tccggaacgc
     2581 caattcgcgc agccgatgct cgatcgtggc gaaatcgaag acggtcatcg tgaaggtcgc
     2641 ggtcgaaggc aggaaggtca cttccgtccc gcgcttggcc ttgccgtcta cgaccggcgc
     2701 cgggccaacg accttgagcg gcgcgaccgt ttcgccatcg gcgaactcga cgacatgctc
     2761 ctgaccgttg cgccagatgc gcagcttgag aaacactgaa agcgcgttga cgactgagac
     2821 gcctacgccg tgcaggccgc cggacacctt gtaggagttc tggtcgaatt taccgcccgc
     2881 gtgaagttgg gtcatgatga cctctgccgc agagacgccc tcttccgagt gaatgtcggt
     2941 cgggatgccg cgtccgtcgt cggtgacggt gaccgagccg tcggcgttga gcgttacggt
     3001 gacccgcgtc gcatggccgg ccagcgcctc gtcgatcgca ttatcgacga cctcatagac
     3061 catatgatgc aggcccgacc cgtcgtcggt gtcgccgata tacatgcccg gccgcttgcg
     3121 cacggcatca agcccgcgga gaaccttgat cgaatcggcg ccgtaagcat cacggtccgg
     3181 gccgggcaaa tcctgcgcat tttcgggcat aggattcggg ttggcgggcg cggattggga
     3241 catgcttttc tggcgctttc tggcttgcga attgggcggg agaatcgtgg ttcaagacca
     3301 ctcaaattat atagtgcaaa acgccgccaa atgcatgtcc tgaaatgcag ttttcccgtc
     3361 tttggcaccc tgtttcgggc cccgcgacgc tctggcgcgg cggctgttct cagacccgaa
     3421 ataaagctga aaaattgccc ggaaattagc cctcatcgcg ggtcagacga agcaacccgg
     3481 acagagcccg ggttgctggt cattgggggc gcgccacggc gcgtcaggca gcgcgttcag
     3541 gcgaccttcg cgttgccctt gccgccggac ttcggaggct cgacgttgcc gaagataagg
     3601 ccctccgggc caccgccgac cttcaccacc tcgccgtcat gaatcttgcc ggcgaggatt
     3661 ttctcggcca gcggatcctg tagcgccttc tggatcacgc gtttcagcgg gcgcgcgcca
     3721 taggccgggt cgtagccctt atcggccagc cattcgcgcg ccgtctcggc catctccaag
     3781 atgatcttgc gatcatcgag cagacgtgag agccgcccca tctggatatc gacgatcgcc
     3841 gccatgtcct gccgccgcag gcgatggaac atgatgatct cgtcgatccg gttgaggaac
     3901 tccggccgga aatgcgcccg cacgatctgc atcacgtcgt cgcggatcgc cgccgtgtcc
     3961 tgtccctccg gctgcatgac cagaagctcg gcgccgaggt tggaggtcat gatgatcagc
     4021 acattgcgga aatcgaccgt gcggccctgt ccgtcggtca gacggccatc gtcgaggact
     4081 tgtaacagga cgttgaaaac gtcgggatgc gccttctcga tctcgtcgaa aagcacgacc
     4141 tgataggggc ggcggcgcac cgcctcggtg agcgccccgc cctcctcgta gccgacatag
     4201 ccgggcggcg cgccgatgag ccgcgcgacc gagtgctttt ccatatattc cgacatgtcg
     4261 acacggatca gcgccgtctc gtcgtcgaac aggaattccg caagcgcctt ggtgagttcg
     4321 gtcttgccga cgccggtcgg tcccaggaac atgaacgaac cgatcggccg gttcgggtcc
     4381 tgcaagcccg cgcgagcccg acgcacagcc gtagccacag cggccaccgc ctccgcctgg
     4441 ccgacgacac gccgggcgag ttcgtcttcc atgcgcaaga gcttgccgcg ctcgccttcc
     4501 agcattttgt cgacgggaac gccggtccag cgcgacacga cgcccgcgac catatcggcc
     4561 gttaccgcct catcgaccag cacttcgctg ccctttgatt cgacgtcggc gagtttcttt
     4621 tcgagatcgg gaatgacgcc ataggtcagc tcgccagcac gctgatattc gccgcgccgc
     4681 tgcgcttgca gaagctcggt gcgggccgct tcaagctgct ccttgatctt ctgcgccgag
     4741 tcgagcttgt ccttctcggc gcgccaacgc gaagtcaaag cggcggattt ctcctccgcc
     4801 tcggcaagtt catgctcaag ccgttgcagc cgatctttcg aggcggtgtc cgtctctttt
     4861 ttcagcgcct cttgctcgat cttcaattgc acgacacgcc ggtcgatctc gtccaattcc
     4921 tcgggcttgg aatcgacctg catccgaagc cgcgacgcgg cctcgtcgac aagatcgatt
     4981 gccttatccg gcaggaagcg gtcggcgata tagcgattgg acaaggtggc cgcggcaacg
     5041 atggcggaat cggtgatccg cacgccgtga tgcagctcgt atttctcttt caggccgcgc
     5101 agaatcgaga tcgtatcctc gacgttcggc tcgttgacga aaaccggctg gaagcgccgc
     5161 gccagcgccg catccttctc gacatgcttg cgatattcgt cgagcgtggt cgcgccgatg
     5221 cagtgcaatt caccgcgcgc caaggccggc ttcaaaaggt tcgaggcgtc cattgcgcca
     5281 tcagccttgc cggcgccgac gagcgtatgc atctcatcga tgaacagaat gatgccgcct
     5341 tgcgccgagg tcacttcgtt gaggatggct ttcagccgtt cctcgaactc gccgcgatat
     5401 ttggcgccgg cgatgagcga gccaagatcg agcgccagca ggcttttgtc gcgcaggctc
     5461 tccggcacat cgccattgac gatgcgtagc gcaaggcctt caacgatggc cgtcttgccg
     5521 acgcccggct caccgatgag cacggggtta ttcttcgtcc gccgtgacag aacctgaatc
     5581 gtgcgacgga tttcttcgtc gcggccgatg accggatcga gctttcccgc gcgcgctgcc
     5641 tcggtgaggt cgcgggcgta tttcttcaac gcgtcatagg cattttcagc cgtcgcattg
     5701 tcggccgtgc ggcccttgcg caaatcctcg atcgccgcgt tcaacacctg tggcgttacg
     5761 ccggcgcttt gcaaaatttt tgcggcttcg gtgcctttct cgagcgccag tgccagcaaa
     5821 agccgttcga cggtgacgta agaatcaccc gctttcttcg cgatctgttc ggcattgtcg
     5881 aaaagccgcg ccgtggccgg tgcgatgtag agctggccgg cgccggagcc ctgcactttc
     5941 ggcagtttcg ccagcgcaag ttctgtttgg gtgagcgctt cttttgcgtt gccgccggct
     6001 ttattgatca acccgctcgc gagcccttcg ggatcgtcga gcagcacctt gagcagatgc
     6061 tcgggcatga attgctggtt gccttcacgg gtcgccagcg attgcgcgct ttgaacaaac
     6121 ccgcgcgcgc gttcggtgta tttttcgaaa tccattctct ccagacctcc caatggcaag
     6181 gctgttcatg cgttctagca tgacgattcc gccccggcac gaccgacagc ggaccttgag
     6241 gggaacttag gaaagtgaat ccacgattgg tagaggtacc gcactgactt tgcgcaaatc
     6301 gatgcagcaa aacagtgcgg cttccccgat tattcctcga gcgacgcctt ggtctccgcc
     6361 acgccgaatt ccggcttgac gcgacgacgg cgacgcggac gcagagcgaa attgttctcc
     6421 tcagcctcgg cctccggccg cgcgagaacc tcggtgccta ccgattcgtc attgaccggc
     6481 agttccggct caggctggac acggacaggc accggcgccg tgataaaggc cgggagcgct
     6541 gcggcagtcg cctcttcgct cacattgcgc ggctcgtgcc gctcatagcg cggggcgtta
     6601 tcatagcgcg gcgcgccgtc accgcggtat ggctgccggg gacgcccatt gccgccctcg
     6661 cggttgtcgc ggggaccatt ctgtccgttg cgctcgccat aagcaggacg ttcctgattg
     6721 cggtcctgat tattgcgatc ctgattccgg tcttgattcc gatcctggaa attgcgcggg
     6781 ccacgttcgc cacgcggctc atattgcggc cgctcctgat aagtcggctg ggcgctgtag
     6841 ttcggctggc cctggccgtt cggcaccgtc ggctgcgcca catagggttg cggctgctgc
     6901 ggaggcggcg tgacactggg agccactggc gacgcgaaac gatccggaag cccgctgaag
     6961 tcgtcctcgt cctcgagctc ctcgttgtcc gtctcggttc ccggacgatt atagcccgcg
     7021 aattgcgcct gctgcgcctg ttgggcggtg gcgatgatgc ggaaataatg ttcggcgtgc
     7081 tgcagatagc tttcggccat cacggggtcg ccggacgatt gtgcgtcgcg ggcgagctgc
     7141 agatattttt cgccgatgtg gtgcgcgg
//
LOCUS       DASWOG010000471         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_157543,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000471 DASWOG010000000
VERSION     DASWOG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157543"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGB93_09710"
     CDS             complement(<1..581)
                     /locus_tag="VGB93_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY0146403.1"
                     /translation="MSSESGADTIFALASGQGRAAIAVLRLSGAGVASILGALVEKAT
                     PPRQAALATFRDPASGEAIDRGLCLFFPAPASFTGEDCAEFHLHGGPAVIAAMFAALA
                     HFPATRPALAGEFSRRAFENGKLDLTEIECIADLIDADTEQQRRQALRQTRGDLRHKA
                     ETWRAALVEAAALLEASIDFADEGDVSPLAQERL"
     gene            595..1787
                     /locus_tag="VGB93_09715"
                     /pseudo
     CDS             595..1787
                     /locus_tag="VGB93_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173984.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
BASE COUNT          353 a          549 c          545 g          340 t
ORIGIN      
        1 agccgctcct gcgccagcgg cgagacatcg ccctcgtcgg caaaatcgat cgaggcttcc
       61 aatagcgctg ccgcttcgac aagcgcggcg cgccaggttt ccgccttgtg ccgcagatcg
      121 ccgcgcgttt gtcgtaacgc ctggcgccgc tgctgctcgg tgtcggcgtc gatgagatcg
      181 gcgatgcact cgatctcggt caggtcgagt ttgccgttct cgaaggcgcg ccgcgaaaat
      241 tcgccagcca gcgcgggccg cgttgcgggg aaatgcgcca gggcggcgaa catcgcagcg
      301 ataacggcag gtccgccgtg aaggtggaat tcggcgcaat cctcgccggt gaaactcgct
      361 ggcgcgggaa aaaagagaca gagcccccgg tcgatggctt cgcccgacgc gggatcacga
      421 aatgtcgcga gggcagcctg ccggggtggc gtcgcctttt cgacaagtgc ccctaatatg
      481 ctggcaacgc ccgccccgga aaggcgtaga acggcaatcg ctgcccgccc ctggccggag
      541 gcgagcgcaa agatcgtgtc ggccccgctt tcgctgctca tctcaaggct cgttatgtcg
      601 ttcaatgaac tcgccaacgc cgccagcccc tatctgctcc aacataagga caatcctgtc
      661 cattggcggc tctgggggcc ggatgccctc gcggatgcgc aaagtgccaa tagaccgatc
      721 ctgctctcgg tcggctatgc cgcctgccat tggtgccatg tgatggccca cgaaagtttc
      781 gaggacgccg aaaccgccgc ggtcatgaac gagcttttcg tgaacatcaa ggtcgatcgc
      841 gaggaacgac cggacatcga ccacatctac atgcaggcgc tccatgcctt tggcgaacaa
      901 ggcggctggc cgatgaccat gtttctgacg ccaaaaggcg agccgttttg gggcggcacc
      961 tattttccga aagactcgcg ctatggccga cccgccttcg tcacggtcct gcgcagtgtc
     1021 gccagagctt tccgcgacga gcaaggtaaa attacctcga atgcggaggc cgtccgcaaa
     1081 gctcttggcg ccgcagctcg cgcaatatgt cgatccaatt gatggcggcc tacgcggtgc
     1141 gccgaaattt cccaatccgt caatcttcga atttttgtgg cgtgcgggcg gtcggcttgg
     1201 cgagaagaat taccgcgatc tcgtccacct gacgctgaca aaaatgtcgg cgggcggcat
     1261 ctacgatcac ttaggcggcg gctatgcgcg ctattcgact gatgaccgct ggctcgtgcc
     1321 gcatttcgag aagatgctct acgacaatgc gcagattgtc gaactgctcg cgctcgcctg
     1381 gcgcgccttc ggcgatgacc ttttcaggca gcgcattgat gaaactattg gctggctaga
     1441 acgcgaaatg acgcggggcg gcttctgcgc ctcgctcgat gccgacagcg aaggcgagga
     1501 aggcaaattc tacgtctgga cgtgggacga actcgtcgaa attcttggcg gcgcggatgc
     1561 cgaattcttc agctcctgtt acgaagcaac gcgcgccggc aattggtccg aaggcggcca
     1621 tcgcgtcatc attctcaatc aattgcagtt gaaagaatcg ccaagcgcca acgaggattc
     1681 acgcctcgcg ccgctgcgac aaaaactttt tgccgcccgc gagacgcgcg tgcatcccgg
     1741 actcgacgac aagatcatgg ccgattggaa tgggctgatg atcgcgg
//
LOCUS       DASWOG010000472         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_305318,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000472 DASWOG010000000
VERSION     DASWOG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305318"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1079
                     /gene="secF"
                     /locus_tag="VGB93_09720"
     CDS             117..1079
                     /gene="secF"
                     /locus_tag="VGB93_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0146404.1"
                     /translation="MKLLRLAPENTKFPFMRFRRVSYPFSAFMSIVSVALFLTLGMNF
                     GIDFSGGTLMELRAKTGAADISAIRSEMRKLTSGDIEVQGFGSPSDVLLRFGVPKEGA
                     AAQEQTVAAVKNALGNQYDFRRVEVVGPRVSGELVQSGTLGVVISIIAVLCYLWFRFE
                     WQFAVGAIIATMHDLLLTVGFFSLTRLEFNTTSIAAILTIVGYSLNETVVVLDRIREM
                     RKKYRKMPTDQLIDVSVNAVLPRTIMTATTVFLALLSLVIFGGEVIRSFSLAMIWGIC
                     VATYSSVFICSPMLIYLGLRNVSAEPAPTGAKDSKAKPGAVKTA"
     gene            1091..1477
                     /locus_tag="VGB93_09725"
     CDS             1091..1477
                     /locus_tag="VGB93_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY0146405.1"
                     /translation="MSGPKYSGFLPGRHVIEGYGNFGFTFGGMSHRGSILALPSGIYA
                     WDVLGPEDLGVGALAQVFAEPKGEIEHLLIGTGVELRPLAKELRVALNRDGIRVEPMA
                     TGAAARTYSILLGENRRIAAALIAVA"
     gene            1482..2294
                     /locus_tag="VGB93_09730"
     CDS             1482..2294
                     /locus_tag="VGB93_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0146406.1"
                     /translation="MDRAADYAYCETQIRRNDPDRWLASLFLPSMLRSHVIALYAFSD
                     EIARVRAHVSEPLLGEIRFQYWRDVITGERGADAPVAAALIDTVERFGLPREKLVSLI
                     DARLFDLYDASMPSMAALESYAAATAGSLFQLAAFILDPAADAAAASRHAGIAYAVTG
                     LLRGLPWQIAAGQTYVPRELYAWPDLKTDRPALDAALARLRALVRQHLMALAAEPAAK
                     AGKAAPAFLPAALCELYLRQMEKPGYDPLTTLIEVPQWRRQWGLWRAARKIG"
BASE COUNT          442 a          779 c          802 g          535 t
ORIGIN      
        1 cgcttgcgct cggaattctg acgaccatcg tgaccgcggt gacgatgacg cgcatgatga
       61 tcgcgctctg gtatcaatat aagcggccaa ccaagctgcc gatctaaggt tttgcgatga
      121 agctgctgcg cctcgccccg gagaatacca agtttccatt catgcggttt cgccgcgtga
      181 gctatccgtt ctccgcgttc atgtcgatcg tgtccgttgc gctgttcctg acgttaggga
      241 tgaatttcgg tatcgatttt tctggcggga cgctgatgga actgcgcgcc aagacgggcg
      301 ctgccgatat ttccgcgatc cgcagcgaaa tgcgcaagct cacgagcggc gatatcgaag
      361 tgcagggctt cggcagcccg tccgatgtgt tgttgcgttt cggcgtgccg aaggaaggcg
      421 cggcggcgca ggagcagaca gttgccgctg tcaaaaatgc gctggggaat cagtatgatt
      481 tccgccgtgt cgaagtcgtc gggccccgcg tctccggcga attggtgcag tcaggcacgc
      541 tcggcgtcgt catctcgatc atcgccgtgc tgtgctatct ctggttccgc ttcgaatggc
      601 agtttgccgt cggcgcgatc atcgcgacaa tgcacgatct tctgctcacc gtcggcttct
      661 tctcgctgac gcggctggaa ttcaacacca catctatcgc cgcaatcctc accatcgtcg
      721 gctattctct gaacgaaacg gtggtcgtgc tcgaccgcat ccgcgagatg cggaagaaat
      781 accgcaaaat gccgaccgat cagttgatcg acgtttcggt gaacgccgtc ctgccacgca
      841 cgatcatgac ggcgacgacg gttttcctcg ccttgctctc gctggtcatt ttcggtggcg
      901 aagtcatccg ctcgttctcg ctcgcgatga tctggggcat ttgcgtcgcg acctattcgt
      961 cggtcttcat ctgctcgccg atgctgatct atctcggctt gcgcaatgta agcgccgaac
     1021 cggccccaac gggcgcgaag gattcgaaag ctaaaccggg cgctgtcaag acggcgtaag
     1081 gagccgacgc atgtccggcc caaaatattc aggctttttg cccggccggc atgtgatcga
     1141 gggctatggc aatttcggct tcaccttcgg cggcatgtcg catcgcggct cgatcctggc
     1201 cctgccgtcc ggcatctatg cctgggatgt gttagggccg gaagatctag gcgtcggcgc
     1261 actggcacag gtcttcgccg aaccgaaagg cgaaatcgaa catctgctga tcggcaccgg
     1321 cgtggaactg cggccattgg ccaaggaact tcgtgttgcg ctcaatcgcg acggaattcg
     1381 cgtcgagccg atggcgacgg gggcggcggc gcggacctat tcgatcctgc tcggtgaaaa
     1441 ccgtcgcatc gcggcggcgc tgatcgccgt ggcttgaggc gatggatcgc gccgccgatt
     1501 acgcatattg cgagacgcag attcggcgca acgatccaga ccgttggctg gcaagcctgt
     1561 ttctgccgtc gatgctgcgg tcgcatgtga ttgcgctcta tgccttcagt gatgagatcg
     1621 cccgcgtccg cgcgcatgtg agcgagcctc tgctcggcga aatccgcttc caatactggc
     1681 gagatgtaat aaccggtgag cgcggtgcgg atgcaccggt cgcggctgct ctgatcgata
     1741 cggtcgaacg ctttgggctg ccgcgcgaaa aactcgtcag cctcatcgat gcgcgcctgt
     1801 ttgatctcta tgatgcgtcg atgccgagca tggcggcgct cgaatcctat gccgcggcga
     1861 cggcggggtc gttgtttcag cttgccgcct ttatccttga tcccgccgcg gacgcagcgg
     1921 cagcatcgcg tcatgccggg atcgcctatg ccgtcaccgg ccttttgcgc gggcttccct
     1981 ggcagatcgc ggcggggcag acttacgtgc cgcgcgagct ttatgcctgg ccggatttga
     2041 aaaccgatcg cccggcgctc gatgccgctc tggcaaggct gcgggcgctg gtgcgccagc
     2101 atctgatggc cttggctgcc gagcctgcgg ccaaagccgg taaggctgcg ccggcgtttc
     2161 ttccggccgc gctttgcgag ctttatctgc ggcagatgga aaagccgggc tacgatccgc
     2221 tcacgaccct tattgaagtt ccgcaatggc ggcgccagtg ggggctctgg cgcgccgccc
     2281 gcaagatcgg ctgagagccg ggccggtttc ccccttgccc ggaatggttt agaacgcgcg
     2341 cacgacatcg cggcgccgcc gcgcccatca ggcccagcaa gaatgaacga acagacgcgg
     2401 gcggccaaga aggccgaagg cccggattat tcaaaaagcc tctatctgcc gcagacggat
     2461 ttcccgatgc gcgccggcct gccgcagaag gagccggaat tgcttacgcg ctggcgcgag
     2521 atcgagcttt accagcgcct gcgcgaagcc gggcagcg
//
LOCUS       DASWOG010000473         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_249725,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000473 DASWOG010000000
VERSION     DASWOG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249725"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1342
                     /gene="hflX"
                     /locus_tag="VGB93_09735"
     CDS             14..1342
                     /gene="hflX"
                     /locus_tag="VGB93_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0146407.1"
                     /translation="MIGPYLNERHKRRADAAAVQGIDNARSAEARIEEARGLALAIDV
                     DVVDAWLVTLTQIRPATYIGPGKVEEIAERTKAEAIDLVVMDCGLSPVQQRNLEKGFA
                     CKVIDRTGLILEIFGRRALTREGTLQVELAHLTYQKSRLVRSWTHLERQRGGFGFLGG
                     PGETQIETDRRLIQERITRIERDLDKVKKTRGLHRKSRNAVPYPIVALVGYTNAGKST
                     LFNRLTRADVLAENMLFATLDPTLRALKLPRGSEIILSDTVGFISDLPTMLIAAFKAT
                     LEEVLEADIILHVRDIAHDDSEAQRTDVDKVLVELGIDIEQPGRVLEVWNKIDAVDAE
                     TALALLNAARRRPANARPVLISALTGQGVDALLSRIETLLSSERVVIELALDPADGQG
                     RHWLYENSEVLSRNEGSDGRLFLTVSVSPALADRVRQRFVEEARPRAGIA"
     gene            complement(1356..2150)
                     /gene="mazG"
                     /locus_tag="VGB93_09740"
     CDS             complement(1356..2150)
                     /gene="mazG"
                     /locus_tag="VGB93_09740"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY0146408.1"
                     /translation="MQKSRNIDRLLEIMTALRDPRTGCPWDVAQTFATIAPYTIEEAY
                     EVANAIGRNDLADLKDELGDLLLQVVFHARMAQEQNAFDFGDVVEAIAAKMIRRHPHV
                     FGAKRDLSRDEVAGLWAKIKAEEKAAQPQEELGLLADVPLALPGLTRAVKLQSKASTV
                     GFDWNDPRLVLDKIREETREIEAALDAKDDVAIQDEIGDLLFAVANLARHVGADPEAS
                     IRGTNAKFERRFGHIETEIGRRGQTLGEVGLAEMDALWNDAKAREK"
     gene            complement(2233..>2766)
                     /locus_tag="VGB93_09745"
     CDS             complement(2233..>2766)
                     /locus_tag="VGB93_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY0146409.1"
                     /translation="GGPDMAPPSPTSPPVDAVVKWFKGDKGFGFVELSNGTGDAFLHI
                     GALQSAGYETVPPGAKLKVYVGNGMKGAQVTRVLEVDTAGASERAPAPRFTEARPARR
                     HPDPSTATEIGGTVKWFDDNKGFGFVQSNDGGKDVFVHISILGPSGVHHLAEGQAVTM
                     RVVDTPKGREALSINLA"
BASE COUNT          548 a          839 c          821 g          558 t
ORIGIN      
        1 gacccgcgct ttcgtgatcg ggccttatct caatgagcgg cacaagcgtc gcgccgatgc
       61 cgctgccgtt cagggaatcg ataatgcacg gtcggcggaa gcgcggatcg aggaagcgcg
      121 cggtctcgcg cttgcgatcg atgttgacgt cgttgatgcc tggctcgtca cgctcacgca
      181 aattcgcccc gcgacctata tcgggcccgg caaagtcgaa gagattgcgg aacgcaccaa
      241 ggccgaggcg attgatctcg tcgtcatgga ttgcggactt tcgccggttc aacagcgcaa
      301 tcttgagaag ggtttcgcct gcaaggtgat cgaccggacg gggctcattc tcgaaatctt
      361 tggccgccgc gcgctgactc gtgaaggtac attgcaagtc gagctcgcac atctgaccta
      421 tcagaaatcg cggcttgtac gctcatggac ccatcttgag cgccaacgcg gcggctttgg
      481 ctttctcggc ggccccggcg agacgcagat tgaaaccgac cggcggctca tccaggagcg
      541 catcacccga atcgagcgcg atctcgataa ggtgaagaaa acgcgcggcc ttcaccggaa
      601 gagtcgtaat gccgtgcctt atcccatcgt cgcgctcgtc ggctacacga acgccggcaa
      661 atcgaccttg tttaaccggt tgacgcgcgc cgacgtgctc gcggagaaca tgctcttcgc
      721 aacgctcgat ccgacattgc gagcgctgaa gctgccgcgc ggaagcgaga ttatcctatc
      781 cgatacagtc ggcttcatct ccgacctgcc gacgatgctg attgctgcct tcaaggccac
      841 tttggaagag gtgctggagg ccgatatcat cctgcatgtg cgcgatatcg cgcatgatga
      901 cagcgaggcg cagcgcaccg atgtcgataa agtgcttgtc gaactcggca tcgacatcga
      961 acaaccgggc cgggttcttg aagtctggaa caagattgac gcggtcgacg ccgagaccgc
     1021 tcttgcgctg cttaacgctg cgcgccggcg ccctgcgaat gcgcggccgg tgctcatctc
     1081 ggcgctcaca gggcagggtg tcgatgccct gctctcgcgc atcgaaactt tgctatcaag
     1141 cgaacgcgtc gtcatcgaac tcgcgctcga tccagccgac ggacaaggcc gccactggct
     1201 ttatgaaaat tccgaggtcc tgagccgcaa tgagggttcg gacggccgtc tgtttctcac
     1261 cgtcagcgtg tctccggcct tggccgacag agtgcggcaa cgtttcgtcg aagaggcgcg
     1321 gccgcgcgct ggcatcgcat agcagtagct aagggctatt tctcgcgtgc cttggcatcg
     1381 ttccagagcg cgtccatttc ggcgagaccg acttcaccca acgtttgacc acggcggccg
     1441 atctctgttt cgatatggcc gaagcggcgc tcgaattttg cgttcgtacc gcgaatcgac
     1501 gcctccggat cggcccccac atgacgcgcc agattggcga cggcgaacag gaggtcaccg
     1561 atctcgtctt gaattgcgac atcgtccttg gcatcgagag cggcttcgat ttcacgggtt
     1621 tcttcgcgga ttttatcgag caccaggcgc ggatcgttcc agtcgaatcc gacggtggac
     1681 gccttgcttt gcaattttac tgcgcgggtc aggccgggta gggccagggg cacatcggcg
     1741 agcagcccca gctcctcttg cggctgcgcg gctttttcct cggctttgat cttggcccat
     1801 agacccgcga cttcgtcgcg cgaaagatcg cgcttggcgc cgaaaacatg cggatgacgg
     1861 cggatcatct tggctgcgat ggcctcgacg acatcaccga aatcaaaggc gttctgctcc
     1921 tgcgccatgc gtgcatgaaa gaccacttgt aggagcaggt cgccgagttc gtctttgaga
     1981 tcggcaagat cattgcgccc gatcgcgttg gcaacctcgt aggcttcttc tattgtgtaa
     2041 ggcgcgatcg tcgcgaaagt ttgcgcgacg tcccatggac agccggttcg cggatcacgc
     2101 aaagccgtca tgatttccaa gagccggtcg atgttgcggg acttttgcat ggccgatcaa
     2161 ataaaatggc cgatcaaaat aaaacggcgc aaggaatcgc ctcgcgccgc gaaatgaaac
     2221 ctggacgaaa cctcaggcga ggttgatcga caaagcttcg cgacccttcg gcgtatcgac
     2281 gacacgcatc gttaccgcct ggccttcggc gagatgatga acgccggacg gccctaggat
     2341 cgagatgtga acgaacacat ctttgccacc gtcattcgac tgaacgaagc cgaagccctt
     2401 gttgtcgtcg aaccacttga ccgttccacc aatttccgtg gcagtcgaag gatcgggatg
     2461 gcggcgcgca ggacgcgctt cagtgaagcg cggcgcaggc gcgcgctcag acgcaccagc
     2521 agtgtcgacc tcaagcacgc gggtcacctg cgcgcccttc atgccattgc cgacgtagac
     2581 cttcagtttg gcccccggcg gcacggtttc ataacctgca ctctgcaagg caccgatgtg
     2641 caggaaggcg tcgccggtcc cattgctcaa ttcgacaaag ccaaagcctt tatcgccttt
     2701 gaaccattta acgacggcat caaccggcgg gctggtcggg ctagggggcg ccatatccgg
     2761 gccacc
//
LOCUS       DASWOG010000474         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_29825,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000474 DASWOG010000000
VERSION     DASWOG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29825"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1554..3665)
                     /gene="scpA"
                     /locus_tag="VGB93_09750"
     CDS             complement(1554..3665)
                     /gene="scpA"
                     /locus_tag="VGB93_09750"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEY0146410.1"
                     /translation="MDAKRPAAMAEAGRLIFSNLKPRPDVHLRMQTPGSAPFLRGARA
                     TMYANQPWSIRQYSGFATAEESNAFFRRNLAAGQTGLSIAFDLPIQRGYDSDDPRVAE
                     DVGKTGVAIDTVEDMKVLFDGIPLARTSVSMTMNGAVLPVFAAFIVAARESGVSSREL
                     SGTIQNDILKEFLVRNTYVYPPQPSMRIVSDVIGHVSREMPRFNAISISGYHMHEAGA
                     TATQELAFTLANALEYVRTALADGLNIDDFAPRLSFFFGIGMDFFMEIAKLRAARLLW
                     VELMAQFAPQLPESSRLRTHCQTSGASLTQEEPYNNIVRATIEAMAAVLGGTQSLHTN
                     SFDEALALPSAQAARTARNTQLILGEESGIPLVVDPLGGSFYVERLTADVAAAARSLI
                     HEIEAIGGMTKAIELGIPQSRIEEAASRRQARVDNCEDIIVGVNKYCAVGPSLVDVRE
                     IDATHVRRQQTERLASVRAARDEGAVIRALAALREAASSGQGTLLDLSIGAMAVRSTV
                     GEVSSALSDVFQRHQAGAYSLETVPSVETPDRRVPGIREKIARFAERKGRAPRILLTK
                     LGQDGHDRGMKVLAGMFKAWGFGVDIAPLFSDATEIVRRAASKDVDVIGVSSHAGGHK
                     ALVAALNAALKAEGVEDIVLICGGIIPPSDGPALEALGVQAIYGPGTSVSEIAADILQ
                     LLEGRRLHVGEKFENDLASAT"
     gene            complement(3788..3961)
                     /locus_tag="VGB93_09755"
                     /pseudo
     CDS             complement(3788..3961)
                     /locus_tag="VGB93_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            4249..4686
                     /locus_tag="VGB93_09760"
     CDS             4249..4686
                     /locus_tag="VGB93_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0146411.1"
                     /translation="MRSTKERTGIVSGFGCNPAKEPSAKNTGGFVADDFQHEIVTSYV
                     IKGKGLVILSSCSHRGIINAVRQAQAATGVEKIHATVGGFHLVPPLTDDYVGRTVMEM
                     KALAPDCIIAAHCSGETFYDIARAEMPGRVVRAAVGTRVDFSA"
     gene            complement(4734..4874)
                     /locus_tag="VGB93_09765"
     CDS             complement(4734..4874)
                     /locus_tag="VGB93_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HEY0146412.1"
                     /translation="MARATSRAWLKRIGWLVLIWTLSVLALGIVALLFRALMKLAGLT
                     TG"
     gene            complement(4874..5881)
                     /gene="cydB"
                     /locus_tag="VGB93_09770"
     CDS             complement(4874..5881)
                     /gene="cydB"
                     /locus_tag="VGB93_09770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0146413.1"
                     /translation="MQLDLSFAWVGIIAFSLAMYVIMDGFDLGIGILFPFVRDRGDRD
                     LMVNTVAPVWDGNETWLVLGAAGLYASFPLAYSIVLSALYLPLILVLAGLIWRGVAFE
                     FRFKADAAHRPFWDKAFAWGSYIATFFQGVSLGAFINGFAVHGPSYEGGALDWLTPFS
                     VFTGLGLIVAYALLGSTWLILKTEGDLQARMRSAAQATTLALLVVVAIVSIWTPLAHP
                     AIAARWFKLPNLIFFAPVPGLTLATAIVLLLSLQQKAHLVPFIAALGLLFLGYSGLGI
                     SLWPHIVPPDLTIEAAAAPPQTQLFVLVGALIIIPVILAYTGFAYYVFRGKVRPGEGY
                     H"
     gene            complement(5885..>6223)
                     /locus_tag="VGB93_09775"
     CDS             complement(5885..>6223)
                     /locus_tag="VGB93_09775"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0146414.1"
                     /translation="LALVMGPAGLIAILSGWITTEMGRQPWVVYGVMRTKDAVSNHSV
                     LALSATLALFVVMYFAVFGTGISYILKLAAHGPDGSGSNVGHTPTPSRPLSAAPDNID
                     PLPADAPRGT"
BASE COUNT         1377 a         1868 c         1851 g         1127 t
ORIGIN      
        1 cttttcgacg gcgggcgtac cgcttgcccg gaacgcggcc gtgatcgaca ccgggctcga
       61 tctcgcgctc acccgtgaca ccaagatcgg catttcttat ttcggccagc tcggctcgaa
      121 ccttcaggac aacgccgtca agggcgactt cctctggcgg ttctgagcgg gaaacggcgg
      181 aaagacagca ccgcaagttc acggcgtcag ggccttttcg aaagtcggcg ttgaaggacc
      241 gggccggtct tcgaatctta gtctgactga ttatccgcca gcgtggcaaa tcgttgccaa
      301 cgccgatcca cgcggtgcag gattcgaaag aacgcgtctc gaatccgcgg tacaattttt
      361 ggggtggaag acccctcatt tgacactttt ttggggtgag gatgacaatc catgggggtg
      421 gaggctgacc aaacttcaat ggcttacggt gacaccatgt ttgccttttg aggtgagcta
      481 cgtgcgttgt ccctcacccc cattaattgt caaaaccgca ctctcattgt cacgtccacc
      541 ccaaaaaatt gtcacggaat tcgagtcgaa cacctggaaa aatgccgttg catcgagccc
      601 tgacggcttg gatatcggaa ccagctcatg ggagcatgca cagaacgcag gaagaaccgt
      661 taggcgagga tccccgcgaa aggagcagcc taatgacctc taataaagag aagaacaaag
      721 accgtgacgc gatcctcgcg gcgtttttga atgaatgtcc gcagccttca ccggctcaga
      781 tcgccgggtg gcgacagcgt tatcctcggt tcgccgcaga tattatgaat cttgctgcgg
      841 acctctacgc tctcgcaaac gctcgcgaga accagccgaa agaccggacg gtagaacgcg
      901 cacgccgcga cgacgaggca gctctggagt cgtttcggaa aaagcgacgc cgcgtcgagc
      961 agtgcctgaa cagcctacgc tcggatgcta taaaagcggc gggtaggagg cgacgcgcgg
     1021 cgttacggag tctgatctcc gggctccaca gcaccgctgt cggcgttggt cagttgccct
     1081 ctcttaaaac tattcggcca gtccgagggc acgcgccttt cgccgcaatt ctttgtatcg
     1141 ggacggcgtc tccgtgaaac cctcaatgag tgcttcgccg tagggcgggt cggagttgcg
     1201 gaaagaatcg aaaggctcgg aaagccgctt gtcatcaata agccccgcaa tcctgcaccg
     1261 atagccaatc gcttgcaggc tgataccata ctcttccttc aaagggatga gagctcggat
     1321 ggtgacatcg cttgcatttc gaaagcgccc cgaacgatat caatcggaag aagaaacgcg
     1381 ccagcaaact ggtgcgccat cgtttccgag gcgtgcggat ctaaaaggtg cgcgagttca
     1441 tgggcgagag tgaataagcg tcagatcgag ctctgcgatg cgaagctaca aagcggatgg
     1501 gcaactcccc attggaagcc ggcccgctcc gtagagtaga ctaatccacc ttgtcacgta
     1561 gcagaggcaa gatcgttctc gaatttctcg ccgacatgga gcctccgtcc ctcgagcagc
     1621 tggagaatgt cggctgcaat ctccgatacg ctcgttcccg gaccatagat cgcctgcacg
     1681 cccaaagcct cgagcgccgg cccgtccgat ggtggaatga tcccaccgca aatcaacacg
     1741 atgtcctcga ctccttcagc cttcagagcc gcattcaagg ccgcgacgag agccttgtga
     1801 ccaccggcat ggctcgaaac cccgatcaca tcaacgtcct tcgaggccgc ccggcgtaca
     1861 atctccgtgg cgtcgctaaa caacggcgcg atatcgacgc caaaacccca cgccttgaac
     1921 atgccggcga ggaccttcat gccccgatcg tgaccgtcct gaccgagctt cgtgagcagg
     1981 atccgcggcg ctcttccctt gcgttcggca aatcgcgcaa ttttctcgcg gatgcccgga
     2041 acgcgtcggt ccggagtctc gactgagggg acagtttcga gggaataggc gccagcttga
     2101 tgtcgctgaa acacgtcgga aagcgcgctc gagacttcgc cgacggtcga gcggacggcc
     2161 attgccccga tggaaagatc cagcagagtc ccctggccgc tcgatgcggc ctcccgcaag
     2221 gccgccagag cgcgaataac ggcaccctcg tcacgcgccg cgcggacgga tgcgagccgc
     2281 tcagtctgtt gccggcgtac gtgtgtcgca tcgatctcgc gaacatcgac gagcgacgga
     2341 ccgaccgcgc aatatttgtt gaccccgaca atgatgtctt cgcaattatc gacacgcgct
     2401 tgccggcggg aagccgcctc ctcgatcctc gattgcggga tgcccaactc aatcgctttc
     2461 gtcattccgc cgatcgcttc aatctcatga atgaggctcc gcgcagcagc cgcgacgtcg
     2521 gcggtcaggc gctcgacgta gaaagaccca ccgagcggat ccaccacaag cgggataccg
     2581 gattcttcgc ctaggataag ttgcgtgttg cgcgccgtgc gggccgcctg cgcactcgga
     2641 agggcaagcg cctcgtcgaa gctgttcgta tgcaacgact gtgttccgcc aaggacggcc
     2701 gccatcgcct cgattgtcgc gcggacgatg ttgttatagg gctcctcctg cgtcagcgac
     2761 gcgccggatg tctggcaatg cgtgcgcagc cggctgctct ccggcagttg cggcgcaaat
     2821 tgcgccatca attccaccca cagaagtcgt gccgcccgca gcttggcgat ctccatgaag
     2881 aaatccatgc caatgccgaa gaagaaggac aggcgcggtg cgaagtcgtc aatatttaga
     2941 ccgtcggcga gagcggttcg aacgtactcg agcgcattgg ccagagtaaa agccagctcc
     3001 tgcgtcgcgg tcgcgcccgc ctcatgcata tgatagccgg aaatcgagat cgcattgaat
     3061 cgcggcatct cgcggctgac gtgcccgatc acatccgaaa cgatccgcat ggatggctga
     3121 ggcggataga catacgtatt gcggacgaga aattctttca ggatgtcgtt ctggatcgtg
     3181 cctgaaagct cgcggctgct gaccccgctt tcccgcgcgg cgacgatgaa ggcagcgaaa
     3241 accggaagga ctgcaccgtt catcgtcatc gacacgctcg tgcgcgcgag cgggatgccg
     3301 tcgaagagga ctttcatatc ctcgaccgtg tcgatggcga cgccggtctt gccaacatcc
     3361 tcggcgacgc gcggatcatc actgtcatag ccgcgctgga tcggcaaatc gaaagcgatg
     3421 gaaagccccg tctgcccggc ggcgaggtta cgccgaaaga aagcattcga ctcttcagcc
     3481 gtggcaaaac cggaatattg ccggatcgac cagggctgat tggcatacat cgtcgcccgc
     3541 gcgccgcgaa ggaacggcgc cgaacccggt gtttgcatcc gcagatgaac gtccggtcgc
     3601 ggctttagat tggaaaaaat aagcctgcct gcttcggcca ttgctgccgg ccgctttgcg
     3661 tccataccag ccaacccttg gctatggcgt gacgctccga agcggtcgcc cgctggcagt
     3721 ccttcgttta tgaagcgagc aggacgctct cgtgtgtgcg gtgtcagtcg gtggggccag
     3781 ccgcgcgtca gcgccaaccg agggcgggcg cgacatgggt gagaattgcc tcgatgacat
     3841 gtgcattgta ggcaacgccc agctgattcc ggaccgtcag gagcagcgtg tcggcctcgg
     3901 cgatcgcttc gtctttcttt agctgctcga tgagcacatc cggttcagca gcatagctac
     3961 gatcgacgcg ctggttttga gccacggtca ttacgatcat ttcggcggcc ttgtcggctt
     4021 tctcaaagca acgcaagggc gcctgaaacc gaatttgccg ctcttcgtcg gcggcgagga
     4081 ttgtttctgc catcggcaat atggcaacgg cgacgattac tgcgttcttg atcggccggc
     4141 catcgtcgcg agcggcctga ggttgatgat gtcaccgcct gcccggcgat cgccgccggg
     4201 caggcggtga catcaggaca gattcaactt gtttcggatg aaaagccgtt gcgttcgacc
     4261 aaagaacgca cggggattgt ctcgggcttt ggttgcaatc ccgcgaaaga gccaagtgcg
     4321 aaaaataccg gcggcttcgt tgcggatgat ttccagcacg agatcgtgac gagctacgtc
     4381 atcaagggca aaggacttgt aatcctcagc tcctgcagcc atcgcggcat catcaatgcc
     4441 gttcgacagg cacaggcggc gaccggcgtt gaaaaaattc acgcgaccgt cggcggcttt
     4501 cacctagtac cgccgctgac ggacgattac gtcggccgca cggtcatgga gatgaaggcc
     4561 ctcgcgccgg attgcatcat cgcggcgcat tgctcgggcg aaacgttcta cgacatcgcc
     4621 cgtgcggaga tgccgggccg cgtcgtccgc gcggccgttg gcacgcgcgt cgatttttcc
     4681 gcgtaagact gacgtcgata aatcggattg attcgacgca cgcacgcggg caactaacct
     4741 gtcgtcagtc cagccaattt catcaaggcg cggaagagaa gagcgacgat tccaagtgcg
     4801 aggacactca gcgtccagat cagaacaagc cagccaatgc gtttaagcca ggcgcgcgac
     4861 gtcgcgcgtg ccatcaatga tagccctcgc ccggacgaac cttgccacga aaaacgtaat
     4921 aagcgaagcc ggtataagcc aggatcaccg gaatgatgat gagcgcgccg acgagcacga
     4981 agagctgagt ttgcggcggc gccgcggccg cttcgatcgt caggtcgggt ggaacgatat
     5041 gcggccacag gctgatgcca aggccactat aaccaaggaa cagcaggccg agcgccgcga
     5101 tgaacggcac aagatgcgct ttctgctgca aggagagcag caacacaatg gccgtcgcca
     5161 gggtcaggcc cggcaccggc gcgaaaaata tgagattcgg caatttgaac cagcgcgccg
     5221 caatcgcggg atgagcgagc ggcgtccaga tgctgacgat cgcaacgacg acgagaagcg
     5281 ccagtgtcgt tgcctgcgcc gcagagcgca tacgcgcctg cagatcgcct tcggtcttta
     5341 atatgagcca tgtgctgccg agcagcgcat aggcgacgat caagccaagg ccggtgaaga
     5401 cactgaacgg cgtcagccaa tccagtgctc caccctcata cgaaggccca tgaacggcaa
     5461 agccgttaat gaaggcgcca agcgagacac cttgaaagaa ggtggcgata taggagcccc
     5521 aggcgaaggc cttgtcccaa aaaggccgat gcgccgcatc tgccttgaaa cggaactcaa
     5581 acgcgacgcc gcgccagatg aggccggcca gcaccaatat cagcggcaga taaagcgcgc
     5641 tcaacacgat cgaataagcg agcggaaaag acgcatagag cccggcggcg cccagcacca
     5701 gccaggtttc attgccgtcc cacacaggcg caaccgtgtt gaccatgaga tcgcggtcac
     5761 cgcggtcgcg cacgaaggga aacaggatac cgataccgag atcgaagcca tccatgatga
     5821 catacatggc gaggctgaag gcgatgatgc caacccaggc gaaggagaga tccagttgca
     5881 tggctcaagt tcctcgcggt gcgtcggccg gcagcggatc gatattatcc ggcgcggcag
     5941 aaagtgggcg cgacggcgtc ggcgtatggc cgacattgct gccgctgccg tccggcccgt
     6001 gcgcggcgag cttgaggatg taggaaattc cggtgccgaa caccgcaaaa tacatcacca
     6061 caaaaagtgc aagtgtcgcc gacagcgcga gaaccgaatg gttcgagacc gcatccttgg
     6121 tccgcattac gccatagacg acccaaggct ggcggcccat ctccgtggtg atccaaccgg
     6181 acagaattgc gatgagcccg gccgggccca taaccagcgc aag
//
LOCUS       DASWOG010000475         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_323406,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000475 DASWOG010000000
VERSION     DASWOG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323406"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGB93_09780"
     CDS             complement(<1..467)
                     /locus_tag="VGB93_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0146415.1"
                     /translation="MRYATHHKDQTRQQLLAVAARALRREGPHRLGVAAIMAEVGLTH
                     GGFYAHFKSKEALIAAALEEIFEERLRIFTTRYEGLAPRDGLARYIDSYLSISHCEKV
                     ENGCALPALAADMPRLGPVARKHFAVGLERLQRSVASFFLRDGSDEQEEMKHAT"
     gene            673..1716
                     /locus_tag="VGB93_09785"
     CDS             673..1716
                     /locus_tag="VGB93_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0146416.1"
                     /translation="MSAKRSALYAGIIAALIAAGWYGYDWWRDGRFIESTDDAYVGGN
                     ITPIAPHVGGIIAKIAVTDNQHVAAGQLLVQIDDRDYRAAADHTRAVQDQHKAALNGL
                     HAQYALQQSTIAKAAADLDAKKAQAIFAKDDAARYRHLALTDAGTSQDAQRSVALDQQ
                     SRAAVAAAQASLVAAKQQLAVLDANIGEAQAAVAQATADLNTAHLNLGYATIRSPIDG
                     FVGNRAAQVGSYVAQGAYLLTIVPARGLWVDANFKEDQLAHMGPHQRANVIADILPGH
                     VFRGHVESLSPGTGAVFSVIPPENATGNFTKIVQRLPVRIALDPDDSSLVRIRPGLST
                     TVSIDTRTQSDAP"
     gene            1713..>3229
                     /locus_tag="VGB93_09790"
     CDS             1713..>3229
                     /locus_tag="VGB93_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0146417.1"
                     /translation="MTQAEPDNWLRSLLPFVVMCIGMFIALLDIQIVASSLQDIGGGL
                     SAAQDEISWVQTAYLVAEIIVIPLSGWLTRVFSTKWLFTFSAAGFTVASVLCGLAWNI
                     ESMILFRALQGLLGASMIPTVFTSSFHYFQGPRRVYSAAVIGTIASVAPTLGPVIGGW
                     ITDTLNWHWLFYVNLVPGIAITLLVPFLVDIDKPDFSLLGEADYPGIVLMAVFLGTLE
                     YVLEEGTRWNWFDDSTIRLCACISAVSGLLFIARSLTFSRPVVDLRAFTNRNFAVGCV
                     LSFITGIGIFATIYLTPLFLGYVRGFSAWQTGEAIFSTGVASMLGVPLYIFFARRFDT
                     RWLMMLGLASFGIAMWTYSFVTHDWGGNQFFLPQILRGLPQVFAVAPSVTLGLGSLSP
                     ERLKYASGLFNMMRNLGGAVGIAVCGAIVNIRTNFHFLGIASNLTPANGAMTRLVDTL
                     GVRYAASPAAQHAWHVVALKRLWLLAYREASTLAYVDAFRAVMIAFLFGTLLVPL"
BASE COUNT          572 a         1001 c          926 g          730 t
ORIGIN      
        1 gtcgcgtgct tcatctcttc ttgctcgtcg cttccatccc tgaggaagaa tgatgcgacg
       61 cttcgctgca agcgttccaa gcccaccgca aaatgcttgc gggcgaccgg tccgaggcgc
      121 ggcatgtcgg cggcaagcgc cggcaaggca cagccgtttt cgactttttc gcaatgagaa
      181 atcgacagat acgaatcgat atagcgcgcg agcccgtctc gcggcgcaag cccttcatat
      241 ctggtcgtaa atatgcggag gcgctcttca aatatctctt cgagcgcggc ggcgataagt
      301 gcttcctttg atttgaaatg tgcgtaaaag ccgccgtgcg tcagcccgac ctcagccatg
      361 atcgccgcca cgccaagccg atgcgggcct tcacggcgaa gagcgcgggc cgccacagcc
      421 agcaattgct ggcgtgtctg gtccttatgg tgggtcgcat agcgcatcag attggcaata
      481 tcgaatttcg cattcggtta cgcgccgtca aacccgcttg aactccagcg cgcgtaatta
      541 tatgactatc atcatataat gcaatcccgc tggctttacc gtggcatcgg cgcggttcga
      601 aattgggtga ttctcgtgga tgactctgtg gtcgcgcgta ccgccctaac cgagcccggc
      661 gctcgcgcga agatgtcggc aaagcggtcg gccctctatg ccgggatcat cgccgcactc
      721 atcgccgcgg gctggtatgg ctatgactgg tggcgcgacg gccgctttat cgaaagcacc
      781 gacgatgcct atgtcggcgg taacataacc cccattgcgc cgcatgtcgg cggcatcatc
      841 gccaagatcg cggtgacgga caatcagcat gttgcagcgg gccaattgct ggttcagatc
      901 gacgatcgcg actatcgcgc cgcggccgat cacacacggg cggtccaaga tcagcacaaa
      961 gccgcgttga atggtctgca cgcgcaatat gcgctccaac aatccacgat cgccaaggcc
     1021 gctgcggatc tcgacgctaa aaaagcgcag gcgatcttcg ccaaggacga tgccgctcgc
     1081 taccggcacc tggcgttgac cgacgccggg accagtcagg atgcgcaaag atcggttgca
     1141 ctagaccagc agtcgcgcgc ggccgtcgcc gcagcgcagg cgagcctcgt ggccgcaaag
     1201 cagcagctcg cggttctgga cgccaatatc ggcgaggcgc aggccgccgt cgcacaggcc
     1261 acggcggacc tcaacacggc ccatctcaat ctcggctatg ccacgatccg gtcgccgatc
     1321 gatggcttcg tcggcaatcg cgccgctcag gtggggtctt acgtcgcgca aggcgcttat
     1381 ctgcttacca ttgtcccggc gcgcggactt tgggtcgacg ccaatttcaa ggaagaccag
     1441 ctcgcccata tggggccgca tcaacgcgcc aacgtgattg ccgacatcct tcccggccac
     1501 gttttccgcg gccatgtcga gagtctttcg ccgggaacgg gcgcggtttt cagcgttatt
     1561 ccaccggaaa acgcgacggg caatttcacc aaaatcgtgc agcgtctccc cgtccggatc
     1621 gcgctcgatc cggatgattc atcgcttgtt cgtatccggc ccggcctttc gacgacggtc
     1681 agcattgata cgcgcacgca aagcgacgcg ccttgaccca ggcagagccg gataattggc
     1741 tgagaagcct gcttcctttc gtcgtgatgt gcatcgggat gttcatcgca ctgctcgaca
     1801 tccagatcgt cgcctcatcg cttcaggaca tcggcggcgg cctctccgct gcgcaggatg
     1861 aaataagctg ggtgcagacg gcctatctgg ttgcggagat catcgtcatt ccgctgtctg
     1921 gctggctgac gcgcgtcttc tcgaccaaat ggctttttac cttctcggca gcaggcttca
     1981 ccgtcgcgag cgttctctgc gggctggcct ggaacatcga aagtatgatc ttgtttcgcg
     2041 cgcttcaggg cctgctcggc gcatcgatga ttccgacggt cttcacatcg tcatttcatt
     2101 attttcaggg accgcgacgc gtctattcgg cagcggtcat cgggaccatc gcatctgtcg
     2161 ccccgacgct gggaccggtg atcggcggct ggatcaccga cacgctcaac tggcactggc
     2221 tcttctatgt caatctcgtg cccggcatcg cgattacgct cctcgtgccc tttctggtcg
     2281 acatagacaa accggacttt tcgttgctgg gagaagcgga ttaccccggc atcgtcctga
     2341 tggcggtgtt cctcgggacg cttgaatacg ttctcgaaga aggcacgcgc tggaattggt
     2401 tcgacgattc caccatccgg ctctgcgcct gtatcagcgc cgtttcgggt ctgctcttta
     2461 tcgcacgcag cctcaccttt tcccgaccgg ttgtggatct gcgcgccttt acgaatcgca
     2521 atttcgcggt tggctgtgta ctctcgttca tcaccggcat cggcatcttt gccacgattt
     2581 atctgacgcc gctctttctt ggttatgtcc gcggcttcag cgcctggcag acgggagaag
     2641 cgatcttctc cacgggcgtc gcctcgatgc tcggcgtgcc gctgtacatt tttttcgcgc
     2701 ggcgctttga cacccgctgg ctgatgatgc ttggcctcgc atccttcggc atcgccatgt
     2761 ggacctacag ttttgtgacg cacgattggg gcggcaatca gttttttcta ccgcagattt
     2821 tacgtggctt gccgcaggtc ttcgcggttg cgccgagcgt cactcttggt ctgggcagcc
     2881 tgtcgccgga aaggctgaaa tatgcgagcg gcctgttcaa catgatgcgc aatctcggcg
     2941 gcgcggtcgg catcgcggtc tgcggtgcca tcgtcaacat ccgcaccaat tttcattttt
     3001 tagggatcgc gtcgaacctg acacccgcaa atggcgccat gacgcggctc gtcgatacat
     3061 tgggcgtgcg ttatgcggcg agtccggccg cgcaacatgc ctggcatgtt gttgcgctaa
     3121 agcgcctctg gcttcttgcc taccgcgaag cttcgacact tgcctatgtg gacgcgtttc
     3181 gcgctgtcat gattgcattt ttgttcggca ccctattggt tccgctcat
//
LOCUS       DASWOG010000476         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_249871,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000476 DASWOG010000000
VERSION     DASWOG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249871"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="mch"
                     /locus_tag="VGB93_09795"
     CDS             complement(<1..765)
                     /gene="mch"
                     /locus_tag="VGB93_09795"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEY0146418.1"
                     /translation="MTEAPKISVNSLAGTIVDRMVAEADKLRIIVSEGSLGETLIDAG
                     VKAVGGFDVGLLMAEICLGGLGSVTLTPYAATAKWPFHLTVRTCDPVIACLASQYAGW
                     ALSHEKWFALGSGPARALALKEPLFQDLGYADKANRATLIIEGDKPPPAEVVAKVAKD
                     TGVDARHLTFVYAPTRSLAGSLQVVARVLEVALHKAHELKFPLSRIIDGIGTAPLSPP
                     HPDFVQAMGRTNDAIIYGGLTHLFVTGPADEARALAE"
     gene            complement(762..1889)
                     /locus_tag="VGB93_09800"
     CDS             complement(762..1889)
                     /locus_tag="VGB93_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0146419.1"
                     /translation="MSEAKAVLIAAASGRALAAAARLSGYRPRVVDFFDDDDTRALAS
                     ATASIVDPAQGFAADDLISRLRELAHGENPIGLVYGSGFEDRPEILAALAKEFFVFGN
                     NAPNVARTKDPRRLAALCRELNIPHPEIRFDPPVDPENWLIKRQGGGGGIHIRPAYAR
                     LEAGDYYQRLVPGAPVSVLLLADGHAAQVLGTSSQWTAESPNLPYRFGGAVRPALGAD
                     AQLAAMSEAGQKIVAAFDLIGLNSVDFLAEANGFHLLEVNPRPGATLDIFMDRDGALF
                     DAHLAACRGHLPRQPLVFTKATATAIAYAPRELPSMPRLAWPDWCKDRQRPGTFLRAG
                     DPVCTLSAEADTAAAARARVGERLALFDDYLLREAQKECAA"
     gene            complement(1882..2769)
                     /locus_tag="VGB93_09805"
     CDS             complement(1882..2769)
                     /locus_tag="VGB93_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEY0146420.1"
                     /translation="MAKLILHMLSPLKHMSPFDVNMALDAGYDSVTPYTSVTLEEIEP
                     LVQDAMFSRSPRDAVRTGVFIAGKDAIQALDMLEKAKKTLLKPFEISLFADPAGSFTT
                     AAAMVACVEKVLKEKKKRSLSGTKVVVYGATGVVGFSSAVIAALEGAHVTLAGYNGPE
                     RVEKATQEIKKRFGVETFFADASDPEKVLNLLVNSEIALCAGRAGVQILSADDVKKAT
                     NLLVCADVNAVPPLGIEGVGLHDDGKELPGGSYGIGALAIGNVKYQTEFRLFKKMATS
                     DAPLCIDFRDAFKLARELV"
     gene            complement(2794..3798)
                     /locus_tag="VGB93_09810"
     CDS             complement(2794..3798)
                     /locus_tag="VGB93_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HEY0146421.1"
                     /translation="MSVQTSARLHLGFFDLNGSLGRRFGSFGLALDRPVTTLTVFRAP
                     ELTVGGPDADRVGAHIEALRSHLHLDATYGARVEQAIPAHAGLGSGTQLAFALAAGLR
                     RLEGLPEDFAGDALLLQRGGRSGIGAALFSRGGLVVDGGHGPHTVLPPVLSHLPFPED
                     WRVILVMDSTFSGVHGAEERAAFAALPTFSASASADICHRLLMQGLPALVERDLGQFG
                     QAVSHIQAILGDYFAPAQGGGRYTSAAVGAVMSRLAHFGAQGIGQSSWGPTGFAFAAS
                     DDEAQRLVRRVREERPAQKAENEGDPVLVVCKAINHGAKITLNSSAQDFKSAPHHSNA
                     "
BASE COUNT          777 a         1198 c         1223 g          657 t
ORIGIN      
        1 ctcggccagt gcacgtgcct cgtcagccgg gccggtgacg aagagatgcg tcaggccgcc
       61 atagatgatc gcatcattgg tgcggcccat ggcctgaacg aaatccggat gcggcggcga
      121 cagcggcgcc gtgccgatgc cgtcgatgat gcgcgacagc gggaatttca gctcgtgcgc
      181 cttatgcagc gcgacttcga gcacgcgcgc aacaacctgc aggctgccgg caaggctgcg
      241 cgttggcgca taaacgaagg tgagatgacg cgcatcgacg cccgtatcct tggccacctt
      301 cgcgacgact tctgccggcg gcggcttgtc gccttcgatg atcagggtcg cgcgattggc
      361 tttgtcggca tagccgagat cctggaatag cggctccttg agcgccagag cccgggccgg
      421 ccccgagccg agcgcgaacc atttttcatg ggaaagagcc cagccggcat attgactggc
      481 gaggcaggcg atgaccggat cgcaggtccg gaccgtcaga tggaacggcc atttcgccgt
      541 cgcggcataa ggcgtcagcg tcaccgaccc gagaccgccg aggcagattt ccgccatcag
      601 cagaccgacg tcaaagccgc cgacagcctt aacaccggca tcgatcaaag tttcgccgag
      661 cgatccctcg gacacgatga tgcgcagctt gtcggcctcc gcgaccatgc ggtcgacgat
      721 cgtgccggcg aggctgttga cgctgatttt cggggcttcg gtcatgcggc gcattccttt
      781 tgagcttcac gcaacagata atcgtcgaag agtgcgagac gttcaccgac gcgcgcacgg
      841 gctgcagcgg cggtgtcggc ctcagccgaa agcgtgcaga cgggatcgcc cgcgcgcaaa
      901 aatgttccgg gcctctgccg gtccttgcac cagtccggcc acgcgagacg aggcatggag
      961 ggaagttcgc ggggcgcata agcgatcgct gttgcggttg ccttggtaaa gaccaatggc
     1021 tgccgcggca agtggccgcg acatgcggcc aaatgtgcgt cgaagagcgc gccgtcgcga
     1081 tccataaaaa tgtcaagcgt ggccccaggg cgcggattca cttcgagcaa atgaaagccg
     1141 ttggcttcgg cgaggaaatc gacgctgttc aggccgataa ggtcgaacgc agcgacgatc
     1201 ttctgaccgg cctcactcat agccgcaagc tgtgcgtcag cgcctaaagc ggggcggacc
     1261 gcgccgccaa accggtaagg caggttcggc gactctgccg tccattgcga agaggtgcca
     1321 agaacttgcg ccgcgtggcc gtccgccagc agcaggaccg agacgggcgc gcccggcacg
     1381 aggcgctgat aataatcgcc ggcctcaagc cgtgcatagg ctgggcggat atgaatacca
     1441 ccgccaccgc cttgacgctt gatcagccaa ttttcgggat cgaccggcgg atcgaaacga
     1501 atctcaggat gtggaatgtt cagctcacgg cagagcgccg cgagacgacg cggatctttt
     1561 gtacgcgcga cgttcggggc gttgttgccg aagacgaaaa attccttagc caacgcggcg
     1621 agaatttcgg ggcgatcttc gaagccgctg ccataaacga ggccgatagg gttttcgccg
     1681 tgcgcaagct cgcgcaaacg tgagatcaga tcatcggcgg caaacccttg cgccggatcg
     1741 acgatcgaag ccgtcgccga tgccagcgcc cgcgtatcgt catcgtcaaa aaaatcgacg
     1801 acgcgcggac gatagcccga aagccgcgcc gcagcggcga gggctcgccc cgaggcagcg
     1861 gcaatcagaa cggctttcgc ctcagacaag ctcgcgcgcg agtttgaacg cgtcgcggaa
     1921 atcgatgcag agcggcgcgt cggacgtcgc catctttttg aagaggcgga attccgtctg
     1981 gtacttcaca ttgccgatcg cgagcgcacc gatgccgtag ctgccgccgg gcaattcctt
     2041 gccatcgtca tgcagaccga caccttcgat gccgagcggc gggacggcgt tgacgtcggc
     2101 gcaaacgaga agattggtcg ccttcttgac atcgtcggcg gagagaattt gcacacccgc
     2161 gcggccagcg cagagtgcta tttcagaatt gaccagcagg ttgagcactt tctccggatc
     2221 gctggcgtcg gcgaagaagg tctcgacacc gaaacgcttc ttgatttcct gcgtcgcctt
     2281 ttcgacgcgc tccgggccat tgtaaccggc gagggtcaca tgcgcgcctt cgagcgcggc
     2341 aatgacagcc gacgagaaac cgacgacgcc ggttgcgcca tagaccacca ctttggtgcc
     2401 ggacagcgaa cgcttcttct tttccttcaa gaccttctcg acgcaggcga ccatcgcggc
     2461 cgctgtcgtg aacgaaccgg ccggatcggc gaaaagcgaa atctcgaacg gcttcagcaa
     2521 agtcttcttg gccttctcga gcatgtcgag cgcctgaatc gcatccttgc cggcgatgaa
     2581 gacgccggtg cgcacggcgt cgcgcggtga gcgggagaac atcgcgtcct gcaccagcgg
     2641 ctcgatctct tcgagcgtga cgctcgtata gggggtcacc gaatcatagc cagcatcgag
     2701 cgccatgttc acgtcgaacg ggctcatgtg tttcagcggg ctcagcatgt gcaggatgag
     2761 tttggccatt atcttgtttc cccgtcttag gtcttaggcg tttgaatggt gaggcgcgct
     2821 tttaaaatcc tgggcggaag aattcagcgt gattttggcg ccgtgattga tggctttgca
     2881 aacgaccaag accgggtccc cctcgttctc cgccttttgc gcgggccttt cttcccgaac
     2941 gcggcgcaca agtcgctgcg cttcatcgtc gctggcggca aaggcaaagc ctgtcggccc
     3001 ccaggagctt tgaccgatac cttgcgcgcc aaaatgtgca agccgggaca tgacggcgcc
     3061 aaccgcggcg ctcgtatagc gtccgccgcc ctgcgccgga gcgaaataat cgccgagaat
     3121 cgcctggatg tggctgaccg cctggccgaa ttgccccaaa tcccgctcga cgagagccgg
     3181 aaggccctgc atgagcagcc gatggcagat gtcggcggac gccgacgctg aaaatgtcgg
     3241 cagagccgcg aaggcggcgc gttcctcggc gccgtgtacc ccgctaaagg tgctgtccat
     3301 cacgagaatg acacgccaat cctcggggaa cggcaaatgg ctcaagaccg gcggcaggac
     3361 cgtgtgcggg ccatggccgc cgtcgacgac caggccgccg cgcgagaata aagcagctcc
     3421 tatgccggag cgcccgcccc gctgcagcag cagagcgtca cccgcgaaat cctccggcaa
     3481 accttcgagc cggcgcagcc ctgccgccaa ggcaaaggcg agctgcgtgc ccgagccgag
     3541 acccgcatgg gcgggaatgg cctgctcgac ccgggcccca taggtcgcgt cgagatgaag
     3601 atgcgaccgc agggcctcga tatgggcgcc gacgcgatcc gcatccggac cgcctacggt
     3661 gagttcgggg gcgcgaaaaa ccgtcagcgt cgtgaccggc cggtcgagcg ccagaccaaa
     3721 actgccgaaa cggcggccaa ggctcccgtt gagatcgaaa aacccgagat gaagacgggc
     3781 ggaggtctgg accgacacgc gagtttgcat tcgtcttcaa cctaacgtga aaaaacttgc
     3841 gccatttatc cggcg
//
LOCUS       DASWOG010000477         5981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_66219,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000477 DASWOG010000000
VERSION     DASWOG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66219"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGB93_09815"
     CDS             complement(<1..648)
                     /locus_tag="VGB93_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0146422.1"
                     /translation="MSDIILKLDHVGVRFDALKAVDDVSLCINCGERRAIIGPNGAGK
                     TTLFNAITGVVRPTSGRVDFDGRDITQLPPHRRAAYGISRTFQITNLFPSLTVRQNME
                     IALRGLKARKFSFFGTSEFKGDEYVQAERALGLSRLGSRAGALVSELSYGEQRQLEMA
                     MALAPGPRLLLLDEPAAGLSPAERVIVTDVIRALPADITIVLIEHDMDLVLSLVDW"
     gene            complement(676..1938)
                     /locus_tag="VGB93_09820"
     CDS             complement(676..1938)
                     /locus_tag="VGB93_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146423.1"
                     /translation="MMDRRSFLKTTGLTLAAGTGVLSAPFIARAQSAPIRIGLMAPQT
                     GVVAAGGREIINGFTLFWDSVGNEAGGRKVEIIVEDDASNPDSALQKARRLVEQANVD
                     FLFGNLLANTGLAVANYVKGNGTPYFIPVIAADDLTQRKRIKNVIRAGGYSASQFSRP
                     LADWALKQGYKKVAMISQDYTFGHEQCGGFAQTFTEGGGTIVGQFWHPLNTSDFSPYL
                     GQVATLQPDAVFAMETGADSARLIQQYASFGLKGQIPLLGSQNAADQSVIRTLGPECE
                     GIVTSAHFAEGADLPATRAFVKTYSDKFGKIPSLYAFSHYVGAMWVAKAINTISGKVA
                     DRDLFIDTVLKTDLPNSPLGKPVRFDQYGNPIYDVFIRKVVKNSDGKYWNVPIETYPQ
                     VSQFWKYDPVTYMKQPPYSRDFQGIKKV"
     gene            complement(1935..2930)
                     /locus_tag="VGB93_09825"
     CDS             complement(1935..2930)
                     /locus_tag="VGB93_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0146424.1"
                     /translation="MTPTQMNAASVGQRVRAHKLRATIWIAAALAALAALPLVLSSYQ
                     TSIATQVLIFSVLAMSIDILAGYAGRTSLGHGAIFGVSTYVAIYWTSSIGGDLWIGIA
                     LGILAATALSAVFGLLAVRTSGVYFLLLTLALGMIVWGVCLRWTNVTGGENGLRGIGR
                     PDFIADDKSFYYAVLIVVSLSTFVMWRFVHSPFGLALRGIRDSESRMRSLGYNVSLHL
                     FLAFLMSGLFAGIAGALYMLFNNFVSPSTVELSQSVSGLLMAVTGGVGTLFGSFVGAA
                     AIIVLENVVSTFTARWPTILGLTFVLIMIFAPEGLVGRARILFARRVQGARWKDK"
     gene            complement(2927..3787)
                     /locus_tag="VGB93_09830"
     CDS             complement(2927..3787)
                     /locus_tag="VGB93_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0146425.1"
                     /translation="MMGINLFQILNGLTFAALLFIVASGFTLIFGLLRIVNLAHGALY
                     LLGGLIGYSIAQETGSFILAVLGAMAAAALLGAALDRGLLRFVRGVELRQVLLTLGVA
                     FILDDLGLVIWGGDTFSVPVPEALRGAMHIGNFFYPKYRIFVLLLGILVFAALWLLMN
                     KTRLGALIRAGVDDPEMVEAMGINIRRVFLGTFMLGAALAGLGGVVGGAFLTLYPSAD
                     AEILVFSLAVVIIGGRGSLWGTALGAVLVGLLNAIGQVIFPELSYFVIFGPMALLLAF
                     RPLGLFGRTA"
     gene            complement(3864..4310)
                     /locus_tag="VGB93_09835"
     CDS             complement(3864..4310)
                     /locus_tag="VGB93_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY0146426.1"
                     /translation="MICNPFWRTRAEDPKATRKSVLRHNIAGMPGVTIMPELYDRAHD
                     LVVDTKKRYDCFIGTDLEFTLRAHGINTLLVTGLNTNSCVLATVVAANVRDFAVIVVK
                     DCVETMDGSALHDAGLLCIRTAFGFVIDTDEIQQLVVFDRASEDAA"
     gene            complement(4329..4517)
                     /locus_tag="VGB93_09840"
     CDS             complement(4329..4517)
                     /locus_tag="VGB93_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146427.1"
                     /translation="MNLGVDHPKAAIIAIDLHRGHLDMGVATMPTSPQVAKQVIAANK
                     RLFDWGRSKSIQIIHLLT"
     gene            4830..5546
                     /locus_tag="VGB93_09845"
     CDS             4830..5546
                     /locus_tag="VGB93_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0146428.1"
                     /translation="MSDSRGREALSTRSESVVGALRTAIMAGQFEPGERLHEVKLTGL
                     LGVSRTPVRAALQRLASEGLLDYTPNRGYTLREFPTGEIVNAYEVRAVLEGLAARQSA
                     ERGLNASELAILRQSLHGGDEMIGSGHVTNEHRTQYSGINASFHETILSSAGSRLLSD
                     MVYLCQQVPVSSPRNVVAFEEGDVRRRHDDHHRIFEAIVGREPWRAEMLMRDHIASVK
                     SSLIRSLSSPAAIHEALRRA"
     gene            complement(5581..>5981)
                     /locus_tag="VGB93_09850"
     CDS             complement(5581..>5981)
                     /locus_tag="VGB93_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146429.1"
                     /translation="VPIFFNTLAAPQPSARRCLELGKIVGDIARRSNKRIALIATGGM
                     SHDPGERRHGWIDEAFDQRFLGEMASGDLEKLAHYTTDDFLAAGAGAVELLGWVALAG
                     ALGAYTSEIVAYEAVKPWATGIGLMSFVPA"
BASE COUNT         1399 a         1761 c         1699 g         1122 t
ORIGIN      
        1 ccaatcgacc aatgacaaca ccagatccat gtcatgctca atcagcacga ttgtgatgtc
       61 cgcgggcaag gcgcggatga catcggtgac gatcacgcgc tcggcgggcg acaggccagc
      121 ggcgggctcg tccagcagca gcaggcgagg accaggcgca agcgccatag ccatttcgag
      181 ttgccgctgc tcgccgtaag agagttcgct caccaaggcg ccggcgcgcg aacccaatcg
      241 cgataagcca agcgcacgct cggcctgcac atattcgtcg cccttgaatt cgctcgttcc
      301 gaagaatgag aatttgcgcg ccttaagccc gcggagagcg atctccatgt tttggcgtac
      361 agtcaggctc ggaaatagat ttgtgatctg aaacgtacgg ctgatgccat aggcggcgcg
      421 gcgatgtggt ggcaattgcg tgatgtcgcg accgtcgaag tcgacgcgac ccgaagtcgg
      481 ccgcacaacg ccggtgatgg cattgaacag cgtcgttttc ccggcgccgt tcgggccgat
      541 gatcgcacgc cgctccccac agttgatgca taacgataca tcatcgacgg cttttaacgc
      601 gtcgaaccgg acaccgacat gatcaagctt gaggataata tctgacacgc gcgcgctcgt
      661 atctgaatgc ctgaatcaga ccttcttgat gccttgaaaa tcacgggagt agggcggctg
      721 tttcatgtag gtcaccggat catatttcca gaactgcgaa acctgcggat aggtctcgat
      781 cggcacgttc cagtacttgc cgtcgctatt cttgacgact tttcggatga atacgtcgta
      841 gatcgggtta ccgtattgat cgaaccgcac tggcttgccc agtgggctgt tgggtagatc
      901 ggtcttaagt accgtatcga taaagagatc gcgatcggcg accttgccgc tgatcgtgtt
      961 gatcgctttc gccacccaca tcgcaccgac ataatgcgaa aaggcataaa gcgacgggat
     1021 ctttccgaat ttgtcggaat aggtcttcac aaaggctcgc gtcgccggca agtcagcgcc
     1081 ctccgcgaaa tgcgccgagg tgacgatgcc ttcgcactca ggccccaacg tgcggataac
     1141 ggactgatcc gccgcattct gcgagccaag gagtggaatt tgccccttca gaccgaagct
     1201 cgcgtactgc tggatcagac gcgcggaatc cgccccggtt tccatcgcga acacggcatc
     1261 gggctggagc gtcgcaacct gcccgaggta aggactgaaa tccgacgtat tcaatggatg
     1321 ccagaactgc cccacaatcg tgccgccgcc ttcggtgaaa gtctgcgcga agccgccgca
     1381 ttgttcatgg ccgaatgtgt aatcctgcga aatcatcgcg actttcttgt aaccctgctt
     1441 gagtgcccaa tcggccagcg gacgcgagaa ttggctggcg ctgtagccgc cggcccgaat
     1501 cacattcttg atccgctttc tctgcgtgag atcgtcagcc gcgatgacgg gaatgaaata
     1561 gggcgttccg ttgcccttca cataatttgc aacggcaagg ccggtgttcg ccagaagatt
     1621 gccgaagagg aaatcaacgt tcgcctgttc aaccagtcgg cgtgccttct gcaaggccga
     1681 atcggggttg gaggcatcgt cttcgacgat gatctcgacc tttcgtccgc ctgcctcgtt
     1741 gccgacgctg tcccaaaaca acgtaaagcc attgataatt tcacggccgc cggcggcgac
     1801 gacacccgtt tggggcgcca tcaggccgat gcgaatcggc gcactctgtg cgcgcgcgat
     1861 gaaaggcgcg gataaaacac cggtgccggc ggcaagtgtg agacccgttg tcttcagaaa
     1921 gcttctgcga tccatcattt atccttccaa cgagcgcctt gaacccgacg agcgaacaga
     1981 atacgtgcac ggccgaccag gccctccggc gcgaaaatca taatgagaac gaaggtaaga
     2041 ccgaggatcg tcggccaacg cgccgtgaag gtgctgacga cattttcaag aacgatgatc
     2101 gccgccgcgc ccacgaatga cccgaagagc gtcccgacac cgcccgtgac tgccatcaaa
     2161 agtccggaaa ccgattgcga cagttcaacc gtcgacggac tgacgaaatt gttgaagagc
     2221 atatagaggg cgccggctat tccggcgaac aaaccggaca tcaggaacgc cagaaacaaa
     2281 tgcagtgaaa cattgtagcc gaggctgcgc atgcgactct ctgaatcgcg gatgccgcgc
     2341 aacgccaggc cgaagggcga atgaacgaat cgccacatca cgaaagtcga cagcgagacg
     2401 acgatcaaga ctgcgtaata gaagctcttg tcgtcagcga tgaagtcggg acgaccgatt
     2461 ccgcgcagac cgttctcgcc acccgtcacg ttcgtccatc gcaggcacac tccccagacg
     2521 atcatgccga gggcaagcgt cagcaggaga aaatagacgc cggacgtccg cactgcgagc
     2581 aggccaaaaa ctgccgaaag tgctgtcgcc gcgaggattc caagcgcgat cccaatccag
     2641 aggtcgccgc cgatcgaaga cgtccaatag atggcgacat aggttgaaac gccgaagatg
     2701 gcgccatgac caagtgaggt gcggccagca tagccggcga gaatgtcgat cgacatcgca
     2761 agcacgctga aaattagaac ctgcgttgcg atcgaggtct gatagctcga tagaacaagc
     2821 ggcaatgcag caagagccgc caaggccgcg gcaatccata ttgttgcgcg caatttatgt
     2881 gcgcgaaccc tctgccccac cgatgcggcg ttcatctgcg tgggcgtcac gcggtcctcc
     2941 cgaagaggcc gagcggacga aatgcgagca gcagcgccat cggtccgaaa atgacgaaat
     3001 aggaaagttc ggggaatatc acctggccga tcgcattcag gagaccgacc agcacggcgc
     3061 ctagagcagt gccccaaagg ctgccacgcc ccccaatgat cacgaccgcg agcgagaaaa
     3121 caagaatctc ggcatcggcg ctcgggtaga gcgtaagaaa ggcgccgccg acgacgccac
     3181 caagtcctgc aagcgccgca ccaagcataa aagttccgag gaagacgcgg cggatattga
     3241 tgcccatcgc ctcgaccatt tcgggatcgt cgacgccggc acggatcagt gcgcctagac
     3301 gggtcttatt catgagaagc catagcgcgg cgaagactag gatgccgagc aggagcacaa
     3361 agatgcggta tttgggatag aagaaattgc ctatgtgcat ggcgccacgc agcgcctcgg
     3421 gaacgggcac cgagaatgtg tcgccacccc agatcacaag gccaagatcg tccaggataa
     3481 aagccacgcc caacgtcagc agaacttgtc gcaattcgac gccacgaaca aaacgcagca
     3541 gccctcggtc gagcgcggca ccaagcagag ccgctgcggc catagcgccc agcacggcga
     3601 gaataaaact gccggtctcc tgggctattg aatatccgat caagccgccg agcagataaa
     3661 gcgcaccgtg cgccaggttg acgatgcgca gaagcccgaa gatcagcgtg aaaccagatg
     3721 ccacgatgaa caacagcgcc gcaaaggtca ggccattcaa aatctggaag agattgattc
     3781 ccatcatgcc ggagcgtcac tctttggcga gctcgacggc gcggcctccg tccttaggtc
     3841 ggatgacgga tgcgtgacga aattcatgcc gcatcctcgc tagctctgtc gaagacaacg
     3901 agctgctgaa tctcatctgt atcgatcacg aaaccaaatg cggtcctgat acagagcagc
     3961 ccagcatcgt gcagggcgga cccatccatg gtttccacgc agtctttcac gacaatcacc
     4021 gcgaaatccc ggacattggc ggccaccacg gtggccagga cacaggagtt tgtattcagg
     4081 ccggttacga gcagcgtatt gattccatgc gcgcgcagcg tgaattcaag atcggtgcca
     4141 atgaagcagt cataccgttt cttcgtatcg accacgaggt cgtgggcgcg atcgtacaat
     4201 tccggcatga tcgtaacgcc gggcattcct gcgatgttgt ggcgcaggac gctcttgcga
     4261 gtcgctttcg gatcctcggc gcgcgtacgc caaaacggat tgcagataat ttccgcctcg
     4321 tcgcggtact atgtcagcag atgaatgatc tggatacttt tggaccgccc ccagtcgaac
     4381 aggcgcttgt tcgctgcgat cacctgtttt gcgacctgag gagaggtcgg catggtcgcg
     4441 acgcccatgt cgagatgacc gcgatgcaga tcgatcgcga tgatggccgc cttgggatga
     4501 tcgacaccga ggttcatcgg agaacctctg cggttccaac aatcggactt tcgcagggcg
     4561 caccacgcgc aacctctccg acccctttgc tcacagcgct tgtcatgacc gccccgagtt
     4621 cgttacccga aactcaatct gcaatacatc cagacaatca gtcaaaataa atgtatacat
     4681 tgatgcgatg gaattgcgca atgctggagc aaatttagcg cataatgcct aaacatatat
     4741 caggtaagtg cccccccccg ccgccgcttg tccgataacc gctatattgt atattatgta
     4801 tacatctggt cttccgattc ggaaaaatta tgagcgatag ccgcgggcgc gaagccttat
     4861 ccaccaggtc tgaatccgtc gttggggcgt tgcgcaccgc gatcatggcc ggtcaattcg
     4921 agcctggcga acgattgcac gaagtcaaac tcaccggttt gctaggcgta tcgcgcaccc
     4981 ccgtccgggc ggctctccag agactcgcat cggagggcct gctcgattac acgccgaacc
     5041 gcggctacac gttgcgcgaa ttcccaaccg gagagatcgt caatgcatac gaagtgcgcg
     5101 cggtacttga gggtcttgct gcgcggcaaa gcgccgagcg aggcctgaat gcgagcgagc
     5161 tcgcaattct tcgccaatcg ctgcacggcg gtgatgagat gattggcagc ggccacgtca
     5221 cgaatgaaca tcggacgcaa tacagtggca tcaatgccag tttccacgaa accattcttt
     5281 cgtcggcggg atcccggctt ctgagcgata tggtttacct gtgtcagcag gtgcccgttt
     5341 cgtcgccacg caatgtcgtc gcattcgaag agggcgacgt gcgtcgccgt catgacgatc
     5401 atcaccgaat atttgaagcg atcgtcggcc gcgagccttg gcgcgccgaa atgttgatgc
     5461 gggaccatat cgccagcgtt aaatcctcgc tgatccgttc gttgtcatcg cctgcggcaa
     5521 tccacgaagc attaaggcgc gcgtgagatc ggcccgatga tttatttagc cagctttaac
     5581 ctaggccggc acaaaagaca tcaggccgat tcccgttgcc cagggcttga cggcttcgta
     5641 ggcgacgatc tcgctggtat aggcgccaag cgcgccggca agcgcgaccc agccaagcag
     5701 ttccacggcg ccggcgccgg ccgctaaaaa atcgtcggtt gtatagtggg caagtttctc
     5761 caggtctccc gaagccatct cacccagaaa acgctggtcg aaagcctcat cgatccaacc
     5821 atgccgccgc tcgcccggat cgtgtgacat accgccggtc gcgatgagcg cgattcgctt
     5881 gttcgagcgt cgcgcgatat cgccgacgat tttgccgagc tccagacaac ggcgcgccga
     5941 aggttgcggc gccgccagtg tattgaagaa gatcgggaca a
//
LOCUS       DASWOG010000478         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_359342,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000478 DASWOG010000000
VERSION     DASWOG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359342"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGB93_09855"
     CDS             <1..293
                     /locus_tag="VGB93_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY0146430.1"
                     /translation="ARYGEPYDPELFEQTEERDFGHALVRTKTLTARAVQEEAFIHAM
                     EHLSYLRAPVDAFFDKVTVNADDSGLRLNRLRLLSALRDAVHRVADFSKISG"
     gene            580..798
                     /locus_tag="VGB93_09860"
     CDS             580..798
                     /locus_tag="VGB93_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146431.1"
                     /translation="MHIEEQGEAAADWPHFVVGRDQLARWVVIETHGLCGGVFRDQAA
                     ALRYAEEECQAYQADFEISREPISLKFP"
     gene            896..1771
                     /locus_tag="VGB93_09865"
     CDS             896..1771
                     /locus_tag="VGB93_09865"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY0146432.1"
                     /translation="MGMTVRGGAFVLRGRDARRLDWRDPYHFALAMSWPRFAALFLGV
                     DLSLNFLFALLYLAQPGSLVNARPGSLTDAFFFSIETLATVGYGVMSPATLYGHVVAT
                     VEIFAGMTFMTIMTGLVFVRFSRPRARVIYAEQAVVGHFNGERTLMIRIGNGRAHAMA
                     ETNAKLSALISEWTSEGQFFRRVYDLNLSRSQIPIFPLTWTLMHKIDETSPLYKYDAE
                     SFPKFVMRLFLSIEARDPMLSAHVYDGKDYDPSEVLFGSRYADAVAVDEQGRTIADLS
                     RISWTEPENAAPEPT"
     gene            complement(1772..2023)
                     /locus_tag="VGB93_09870"
     CDS             complement(1772..2023)
                     /locus_tag="VGB93_09870"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0146433.1"
                     /translation="MSTDQNDDISTLSFETAMKELESIVERLEKGNVELEESIAIYAR
                     GEALKKRCDDLLKNAEEKIQKITFGADGKPAGTAPLDAE"
     gene            complement(2053..>2268)
                     /locus_tag="VGB93_09875"
     CDS             complement(2053..>2268)
                     /locus_tag="VGB93_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEY0146434.1"
                     /translation="YIAACALKSSADETLIARQLRRASPTATPNMHLVALADRILARD
                     GRMSAAIKQIGRGADCFEAEPFVLELD"
BASE COUNT          408 a          665 c          682 g          513 t
ORIGIN      
        1 gggcgcgcta tggcgagccg tacgacccgg agctttttga gcaaactgaa gaacgtgact
       61 ttggccatgc tttggtgcgg acaaaaactt tgacggcaag ggccgtgcaa gaggaggctt
      121 tcatacacgc gatggaacac ctgtcttatc ttcgcgcccc ggtcgatgcc tttttcgaca
      181 aggttacggt caacgccgac gattcggggc tgcgactgaa ccggctgcga ctgctcagcg
      241 cgttgcgcga cgccgttcac cgcgttgccg acttctccaa aatctcgggt taagagactg
      301 ccgggcaaag aattttcctg gcagttatca tccctatatt tcgcgccgtt tcgatccctc
      361 tcgaatctat atggccgctt cgccagattc cgggcgctgc cgaggtttcg gcgttggaga
      421 ccaagatgag atcgagagca cgtgctcgga atgctgccac ggagcctagt attatcatgc
      481 ggcccgaagt tttatttgca ggcgtcgtcg cctgcatcac cgttttttgc atgcttgtgt
      541 gtgccgccga tcttctgtcc ggccattgaa agaaggccga tgcacatcga agagcaaggc
      601 gaggcggctg ccgattggcc gcattttgtt gtcgggcgcg atcaattggc gcgctgggtt
      661 gtcatcgaga cgcatggcct ctgcggcggc gtcttccgcg atcaggcggc ggcgctacgc
      721 tacgccgagg aagaatgcca ggcctaccaa gctgatttcg agatcagccg cgagccgatc
      781 agcctgaaat tcccttaaaa acgtccccct cgacattctc cgccgattcg ctaccaaagc
      841 cggatcggcg gaggtcgcgt ttatggcgaa cgagaaggcg aggaaactaa agtccatggg
      901 catgacggtc cgtggcggcg ccttcgtgct ccgcgggcgc gatgcgcgcc ggctcgattg
      961 gcgcgatccc tatcattttg cactggcgat gagctggcca cgttttgcgg ctctgtttct
     1021 tggcgtcgat ctttcgctca actttctctt cgcgctgctt tatctggcgc aaccgggcag
     1081 tcttgttaac gcgcggccgg gctcgttgac cgatgcgttc ttcttcagca tcgagacgct
     1141 ggcgacagtc ggctatggcg tgatgtcgcc cgcgacgctt tacggccatg ttgtcgcgac
     1201 ggtcgaaatc ttcgccggca tgactttcat gacaatcatg accggccttg tgttcgtgcg
     1261 attctcgcgg ccgcgcgcgc gggtcattta cgctgagcaa gcggtcgtcg ggcatttcaa
     1321 tggcgaacgc acgctgatga tccgcatcgg caacgggcgt gcccacgcca tggcggagac
     1381 caacgcgaag ctttccgcgc tgatcagcga gtggacgtcc gaaggccaat tcttccgccg
     1441 cgtctatgat ctcaacttgt cgcggtcgca gatcccgatc ttcccgctga catggacgct
     1501 gatgcacaag atcgacgaga cgagtccact ttacaaatac gatgcagaaa gctttccgaa
     1561 attcgtgatg cggctgttcc tctcgatcga ggcgcgcgat ccgatgcttt cggcgcatgt
     1621 ctacgacggc aaagattacg atccgagcga agttcttttc ggcagccgtt atgccgatgc
     1681 cgtcgcggtc gacgagcagg gacgcacgat tgccgatctg tcccgtatca gctggacgga
     1741 gccggaaaat gcggcccccg agccgacctg attactcggc gtcgaggggt gcggtgccgg
     1801 ccggcttgcc gtcggcgccg aatgtgatct tctgaatttt ttcctcggcg tttttcagca
     1861 gatcatcgca gcgcttcttc aaagcctcgc cgcgtgcgta gatcgcgatc gattcctcaa
     1921 gctcgacatt gcccttttcc agccgctcga caatcgactc gagttccttc atcgcggtct
     1981 cgaagctcag cgtgctgatg tcgtcgtttt gatcggtgct cacatttggc tcgcgtcgga
     2041 aagagaaacg ggtcagtcga gttcaagtac gaagggttcg gcctcgaaac agtccgcgcc
     2101 gcggccgatt tgcttgatgg ccgcgctcat gcgtccgtcg cgggccagga tgcggtccgc
     2161 caaggcaacg agatgcatgt tcggcgtcgc ggtgggcgac gcgcgccgca attgccgcgc
     2221 gatcaaggtc tcgtccgcgc tggatttgag cgcgcaggcc gcgatata
//
LOCUS       DASWOG010000479         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_176446,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000479 DASWOG010000000
VERSION     DASWOG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176446"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..899)
                     /locus_tag="VGB93_09880"
     CDS             complement(120..899)
                     /locus_tag="VGB93_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEY0146435.1"
                     /translation="MIDFITTAPLWAQTFWILIVPTIIFMFGPFVVRRFVALESLSTN
                     NEVAGFKFATVGVLYAVLLAFSIIIVWEKFNDAEATVEREAAAAATIYHLVPGLDPAQ
                     AVATRDALTAYLKSVVTEEWSAMEQGRANRSTWEALGNIYTALLTNDQTAKGDATVKA
                     EIFNQLNTLTQARRTRLRAAEGDVPGPLWIVLFGGAVLTIVFTFFFGTKNLRAQTLMT
                     GFLSVLIFSELLIAVALDRPFTGGIREPPDAIVHVLNDFSP"
     gene            complement(1009..1614)
                     /locus_tag="VGB93_09885"
     CDS             complement(1009..1614)
                     /locus_tag="VGB93_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0146436.1"
                     /translation="MSKIAAIILAAGRSTRFGDDRSTTKLVAQFGGAALVRRVDEAAL
                     ASRASPVIVVTGQARDLIASALKDCDVRLVDAPDYAAGLSRSLRAGVAAVPVDAEGAI
                     ILLADMPLIAPALIDALIATFEAAPRATAVVPTYAGRRGNPVLLARTLFSTIAKLDGD
                     RGAGQILAEIEGVVECPVWDESIFVDVDTPDDLRHLAEPRG"
     gene            complement(1611..2312)
                     /locus_tag="VGB93_09890"
     CDS             complement(1611..2312)
                     /locus_tag="VGB93_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0146437.1"
                     /translation="MRASLLAELNAERESRHASILVTDIATGAQRLVHDTEVDRDPLA
                     PQLREQIRLGKSALVESGEATFFLRVRVPTLRMVVVGAVHIAQGLTPIARLAGYEVIV
                     IDPRSAFATPERFEDCEVIAQWPQDVFATRAVDRYTAMVVLSHVPDIDDEGIKAALAG
                     ECFYIGALGSRKTNAARLERLRVQNISEAALARIHAPIGLDIGAVGATEIAIAIVAEV
                     IAARRQKPLRSEQAA"
     gene            complement(2312..2635)
                     /locus_tag="VGB93_09895"
     CDS             complement(2312..2635)
                     /locus_tag="VGB93_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0146438.1"
                     /translation="MLAADDEILQTALEWHTQSGQVAIATVVETWGSAPRPVGSQLVV
                     GSDGRFVGSVSGGCIEADVITEALDVIASGQPKLREFGVSDETAWRAGLSCGGRIKIF
                     VERVD"
     gene            complement(2698..3243)
                     /locus_tag="VGB93_09900"
     CDS             complement(2698..3243)
                     /locus_tag="VGB93_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003554634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0146439.1"
                     /translation="MRAVPVLSICLLLSMPVLGSSAGAQSLTEKTGINSLIGRAPSTQ
                     DFVTEAGISDMFEIQSSKLALDKVSDDAIKTFARHMVTDHGKTTAKLEDMVDSGKVKA
                     KPPTVLDSSHQSLLDKLKSLSGADFKKEYLDDQVKGHKDAVDLFKRYSKGGTNPDLKQ
                     FAETVLPTIERHLDMAQTLDK"
     gene            3815..>4648
                     /locus_tag="VGB93_09905"
     CDS             3815..>4648
                     /locus_tag="VGB93_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0146440.1"
                     /translation="MTIQSDQSIDIHATDHLGYSAGGLKRETGWWGAFVIGLAGTILV
                     TGIAPVMVTSLGASSIPITVFITLTGWLLCLFLAELAAMMPERTGGSPSYAYPAFVSR
                     WPKFAKHVNGFTAWAYWLGWFPVAPLNMILAAAYLADRFGFNTTAGFTPIHTFIAWST
                     LVISVIGILLFFIPAYRGIKFGAAFTTVLALLSMIPLTFIAVAWIFNPGVVHFNELAG
                     FKHVDGTSFFSPISGFNWFEVYIAYGFLLTWNVIAMEAAACDIGECTNPDHDAKIAMN
                     HE"
BASE COUNT         1002 a         1370 c         1291 g          985 t
ORIGIN      
        1 acagcaatca tcaataaact tgaggcacga gccgcgcttt gcgcatcttt cccccgattc
       61 gagtttttta agctgtccga ccagacgctt gaattacggc ctcggggcgg cagcgtgaac
      121 tagggtgaga agtcattcag cacgtggacg atggcgtcag gcggttcgcg gatgccgcct
      181 gtgaacggtc ggtcgagcgc gacagcgatc aacagctcgg agaaaatcag cacgctcaag
      241 aagccggtca tcaatgtctg tgcgcgcaga ttttttgtgc cgaagaagaa ggtgaaaacg
      301 atcgtcagaa ccgcgccacc gaagagaacg atccaaagag gccctggcac gtcaccttcg
      361 gccgcgcgta gcctcgtccg tcgcgcctgt gtcaatgtgt taagctgatt gaaaatttcg
      421 gctttgaccg ttgcgtcgcc tttcgcggtt tggtcattcg tcagcagcgc tgtgtaaatg
      481 ttgccgaggg cctcccaagt cgatcggttc gctcgcccct gctccatcgc cgaccattct
      541 tcggtgacga ccgatttcag gtaagcagtc agcgcgtccc gcgtcgcgac ggcctgtgcg
      601 ggatcaaggc ccggaaccaa atgataaatg gtggccgcag cggctgcctc ccgctcgacc
      661 gtcgcctcgg catcgttgaa tttctcccac acaatgatga tcgagaaagc gagaagcaca
      721 gcatacaaca cgccgacggt cgcgaatttg aacccggcga cctcattgtt cgtggaaagg
      781 ctctcaagcg ccacaaaccg ccggacgacg aacggtccga acataaagat gatcgtcggc
      841 acaatcagaa tccaaaaggt ctgtgcccag agcggcgcgg tggtgatgaa atcgatcata
      901 cgtgactctt cgtgtggcgg ggcgcagcca tctccgctcc atcttcactt ataggagctg
      961 ggacttgggc ggagattgcg gcaacccgtt tgacatttca actcgccatt aacctcgggg
     1021 ctcagcgaga tggcgcagat cgtccggtgt atccacgtcg acaaaaattg attcgtccca
     1081 aacgggacat tcgacgacac cttcaatctc cgcaagaatt tgtccagcac cgcggtcgcc
     1141 atcgagcttt gcaatcgtgg agaaaagcgt ccgcgcgaga aggaccggat tgccacggcg
     1201 gccggcatag gtggggacga ctgctgttgc gcgcggcgcc gcctcgaatg tggcaatcaa
     1261 agcgtcgatc agcgccgggg cgatgagcgg catatcggcg agcaaaatta tcgcaccttc
     1321 agcatcaacg ggcacggcgg cgacgcccgc ccgcaacgat cgcgagagtc ccgcagcata
     1381 atccggcgca tcgacgagcc gcacgtcgca atctttaagc gccgacgcta tcaggtccct
     1441 ggcctggccg gtcacgacga tgaccggaga cgcgcgcgac gccagtgctg cttcgtctac
     1501 acggcgcaca agcgctgcgc cgccgaattg agctaccagc tttgtcgtcg acctatcgtc
     1561 gccaaaacgc gtcgagcggc cggccgcaag aatgatcgcg gcgattttgc tcatgcggcc
     1621 tgttccgacc gcagcggctt ttgccgccgc gccgcaatga cctcagcaac gatcgcaatt
     1681 gcgatctcag ttgcgccgac ggcgccaatg tcgagaccga tcggcgcatg gattcgcgcg
     1741 agcgccgcct cggaaatgtt ctgcacgcgc aagcgttcga gccgcgcagc gtttgttttg
     1801 cgcgagccga gcgcaccgat atagaagcat tcgccagcga gcgccgcctt gataccttca
     1861 tcgtcgatat cgggaacatg cgacagcacc accatcgcgg tgtagcgatc gacagcgcgc
     1921 gtcgcaaata cgtcttgcgg ccattgcgcg atcacttcgc aatcctcaaa cctttccggc
     1981 gtagcaaagg cactgcgcgg atcgatcaca atgacctcat acccggcgag acgcgcaatg
     2041 ggtgtcaagc cctgcgcgat atgaacagcg ccgacaacga ccatgcgcag cgtaggtacg
     2101 cgcacacgca ggaaaaacgt agcttccccg ctttcgacca atgcactctt gccgagacga
     2161 atctgctccc gcaattgcgg cgcgagcgga tctcgatcga cctctgtgtc gtggacgagg
     2221 cgctgcgcgc ccgtggcaat gtcggtgacc agaatggacg cgtgccgtga ctcgcgctcg
     2281 gcgttcagtt cggcgagaag agaagcgcgc atcagtcaac gcgctcgacg aaaattttta
     2341 tgcggccccc gcacgacaga ccagcacgcc acgccgtttc atcgctgacg ccaaattcgc
     2401 gcagctttgg ctgaccactc gctatgacat ctagcgcctc ggtaatgaca tcggcttcga
     2461 tacagcctcc agaaacggag ccgacaaaac ggccgtcgct gccgacgacc aattggctgc
     2521 cgaccgggcg cggcgccgat ccccaggtct caacaacggt ggcaatggcg acttgtccgc
     2581 tttgtgtgtg ccattcgagg gcggtctgaa ggatttcgtc atcagcagca agcataaagg
     2641 cctcacgaaa ttcgtgaggc ctttatatca tgtaaactcg cgggaaaacg agcttcttta
     2701 cttgtcgaga gtctgcgcca tgtctaggtg gcgctcgatc gtcggcaaca cggtctccgc
     2761 aaattgcttg agatcgggat tcgtcccgcc cttggaatag cgcttgaaca ggtcaacggc
     2821 gtccttatgc cctttgacct gatcgtcgag atattctttt ttgaaatccg cgccgctcag
     2881 cgatttcagc ttgtcgagca gggattgatg cgaactgtcg agcacagtcg gcggtttcgc
     2941 tttgactttg cctgaatcga ccatatcctc gagtttggcg gtcgttttgc cgtggtccgt
     3001 gaccatatgc cgagcgaaag tcttgatcgc gtcgtcgctg accttatcca gcgccagctt
     3061 gctcgattga atttcgaaca tgtcgctgat gccggcttca gtaacgaaat cctgcgtcga
     3121 tggcgccctg ccgatcaaag agtttatgcc agtcttttca gtcagcgact gggcccccgc
     3181 cgatgaacct agcaccggca tacttagtag aaggcaaatt gataatacag gaacggcccg
     3241 cattttaact cctcgcgttc agtcgcaccg acaactggag gcgggaatga aagttccggg
     3301 accgctaagc gcttgcgacg acggtttgat caccgcttga tctattactc gtattttgtt
     3361 gtgcctgacc ctcacgcgct tcgacctgca tcttcgcccg cgcgatgttc tgttccagca
     3421 atggccaagt cgaaaacgaa acacatgtgt gaaagctcgt gctcgctcgc tgatcgcttt
     3481 gcgacgcaaa aaccgcaatg caaaactgcg ctgcactgcg aagcgcaaat tgaatctgtc
     3541 aaacatcgcg gcaaattgca tcgcaaatag gcaatgaagc gtgcgaacac ccgcaaaatt
     3601 tcattgtgga aaaagagttt ctttacggga atgatttttc gaagcatcct gccgtcgtga
     3661 ggaaacgaca taagcaaaag ccggtatcga gacgtctgcg attccgcgat gaataaaaaa
     3721 acagcatcgc gggtggcacg tgtctaacaa ggcacgctcg atcgtatgaa aaataaaaac
     3781 atctcggcgc cggcaaccaa ggagcgcgag accaatgaca attcagtctg atcaaagcat
     3841 agatattcat gcaaccgatc acttgggtta cagtgcgggt ggcctaaaac gcgaaaccgg
     3901 atggtggggc gctttcgtca tcggcttggc cggcaccatt ctggtgacgg gcatcgcgcc
     3961 ggtgatggtc acctcactcg gcgcttcgtc gattccgatt accgtcttca tcacactgac
     4021 gggttggctg ctttgcctct tcctcgccga gcttgcggcg atgatgccgg agcgtaccgg
     4081 cgggtcgccg tcgtatgcct acccggcctt cgtctcccgc tggccgaaat ttgccaaaca
     4141 cgtcaatggc tttacggcct gggcttactg gcttggctgg ttcccggtgg cgccactcaa
     4201 tatgatccta gcggctgcct atctcgccga ccgtttcggt ttcaacacca cggccggatt
     4261 caccccgatc catacgttca tcgcctggtc gacgttggtc atctcggtga tcggtatcct
     4321 gctgttcttc attccggcgt accgggggat taagttcggc gctgccttca cgactgtgct
     4381 agctttgctt tcgatgattc cgctgacgtt cattgcggtc gcctggattt tcaatcccgg
     4441 tgtcgtccat ttcaacgagc tcgccgggtt caaacatgtg gacggaacaa gcttcttttc
     4501 accgatcagc ggtttcaact ggttcgaggt ctacatcgcc tatggcttcc tgctgacatg
     4561 gaacgtgatc gcgatggaag cggccgcttg cgatatcggc gaatgtacta atccagatca
     4621 tgacgccaag atcgccatga accacgaa
//
LOCUS       DASWOG010000480         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_305652,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000480 DASWOG010000000
VERSION     DASWOG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305652"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VGB93_09910"
     CDS             <1..564
                     /locus_tag="VGB93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0146441.1"
                     /translation="SSAIEVACALIGELYRVADDLKTAGDPSGRFDPPCSTIHVGIIR
                     GGTARNILAKECVFEWEFRGLPNVDYNLALAHMNAQIARIVAEKAADPTKRARVETAA
                     HHPVPGLNPEPGSLAENLGLKLARRNATTTVSYASEAGQFQTAGIPTVICGPGSIDQA
                     HQPDEYIEIDQLAAGVTFMRRLVAELS"
     gene            complement(580..1257)
                     /locus_tag="VGB93_09915"
     CDS             complement(580..1257)
                     /locus_tag="VGB93_09915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0146442.1"
                     /translation="MSWRNFFFADRASRASDRYPAIYYEDIGKAIAAQIPIPEAVVLD
                     YACGAAPAASLIAEHCATAYLYDSTPKMQAILRQRYAANPKVKILTEAQVRALPDGTL
                     DMVLFNGLLQYLTLAQCTEAVDFAAAKLRLNGRLIVADVIPPHADPLSDSWALVEYAY
                     RGGFLLAALRGALSRSRPLRRHIPLTTFTIPDMQRLLATHGFETRRADRNIGHNQMHM
                     TFLAKRL"
     gene            complement(1289..2272)
                     /gene="dmeF"
                     /locus_tag="VGB93_09920"
     CDS             complement(1289..2272)
                     /gene="dmeF"
                     /locus_tag="VGB93_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEY0146443.1"
                     /translation="MHDHANDGMNHPHVFLGAEHAANERRTLAVVALTVAMMFGEIIG
                     GLAFRSMGLVADGLHMSTHAAALGVSAFAYWYARRHANDPRFAFGTGKVGDLAAFTSG
                     LGLAMVAVLIAYESAIRLFHPQVIVYNEALMLAALGLCVNIASAWLLRDSHDHGHSHG
                     HTHDHAEAHDHDRDHDHDHDHPQGEQDLNLRAAYVHVIADAAVSILVITALALARIFG
                     WVWLDPAVGLVGSVVIISWAYGLLRAAGGILLDMTPDEKPKETIRARLERNGDYVSDL
                     HLWRVGPGHRAAMVSIVSSNPQPTEAYRARLADMPGLSHVTIEVLTCPEEH"
     gene            complement(2302..>2855)
                     /locus_tag="VGB93_09925"
     CDS             complement(2302..>2855)
                     /locus_tag="VGB93_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY0146444.1"
                     /translation="SGEPPPPPPGPPPGFGPNDADQGAPGVPNNSTTVTLDVPPRPVV
                     LLAAKARWDGGFKTIKDILDRVKAAADKAGLKITGHPLAMFTQEDDNGFGFTAMLPIA
                     AGSKATLTDGITLGQSPAGKAIKFQHRGPYDDIDSTYDLITAYLDEKGLEAQNFFIEE
                     YLTSLTSAEDPYLGVDIYVFLKP"
BASE COUNT          613 a          863 c          845 g          534 t
ORIGIN      
        1 tcgagtgcga tcgaagtcgc ctgcgctttg atcggcgagc tttatcgtgt ggccgacgac
       61 ctcaagacgg cgggcgatcc cagcgggcgt tttgatccgc cctgctcgac catccatgtc
      121 ggtatcatcc gcggcggcac ggcgcgcaac atcctcgcca aggaatgcgt gttcgaatgg
      181 gagtttcgcg gcctccctaa tgtcgattac aaccttgcgc tggcacatat gaatgcgcag
      241 atcgcgcgga tcgtcgccga aaaagccgcg gacccgacaa agcgcgcccg tgtcgagacg
      301 gccgcacatc atcccgtacc gggtctcaat ccagagccgg gatctttggc cgaaaatctg
      361 gggctgaaac tggcgcgccg caacgcgacg accaccgtct cctatgccag cgaagcaggg
      421 caattccaga ctgccggcat tcctacggtc atctgcggcc cgggatcgat cgatcaggcg
      481 catcagccgg atgaatatat cgagatcgac caattggcgg cgggcgtcac gttcatgcgt
      541 cggcttgtgg ctgagctgag ctagtagcca acctctcggt cacaggcgct tcgccagaaa
      601 cgtcatatgc atctgattgt ggccgatatt gcgatcggcc cggcgcgttt cgaaaccatg
      661 cgttgcgagc agccgttgca tatcggggat agtgaaagtc gtcagcggaa tgtggcgccg
      721 aagcggccgc gagcgcgaca gtgccccgcg caaagccgca agcaggaagc cgccgcgata
      781 cgcatattcg acgagcgccc atgaatcgct cagcgggtcg gcatgcggcg ggatgacatc
      841 ggcgacgatg agccggccat tcagccgcaa tttcgccgcg gcgaaatcga ccgcttccgt
      901 gcattgcgcc aacgttaggt attgaagcag accattgaac aaaaccatgt cgagcgttcc
      961 atccggcaac gccctgacct gcgcctcggt tagaattttg acctttggat tggccgcata
     1021 acgttgacgc aagatcgcct gcatcttcgg tgtggaatcg tagagatagg ccgtcgcgca
     1081 atgttcggcg atgagcgaag cggctggcgc tgcgccgcag gcataatcga gaacgaccgc
     1141 ctcggggata ggaatttggg cggcgatcgc cttgccgata tcttcgtaat aaatcgccgg
     1201 atagcgatcg ctcgcccggc tcgcgcgatc ggcaaagaaa aaattacgcc agctcatatt
     1261 tcgcgccaat caaaacccgg cacatgcctc aatgttcttc cggacacgtt agaacttcga
     1321 tcgttacatg gctcagaccc ggcatatccg ccagacgcgc cctgtaggct tcggtcggct
     1381 gcggattcga actgacgatc gaaaccatcg cggcccgatg gcccggacca acccgccaca
     1441 gatgcaaatc ggaaacgtaa tcgccattgc gctcaagccg cgcgcggatg gtttccttcg
     1501 gtttctcgtc cggcgtcatg tcgaggagga taccgcccgc agcccgcagc aaaccataag
     1561 cccaactgat gatgaccacg gaaccgacca gaccgactgc aggatcgagc cacacccagc
     1621 cgaaaatacg cgccagcgcc aaagcagtga tgacaagaat ggagaccgcc gcgtcggcga
     1681 tcacatgcac gtaggccgca cgcagattga gatcctgctc gccttgtgga tgatcatgat
     1741 catgatcatg atcgcggtca tggtcatgcg cttcggcgtg atcatgcgta tgtccgtgag
     1801 aatgcccgtg gtcgtgcgag tcgcgcagca gccaggcact tgcgatattc acacagaggc
     1861 caagcgcggc aagcatcaaa gcttcattgt aaacgatcac ctgcggatga aagagccgga
     1921 tggcgctttc gtaagcgatc agcaccgcga ccatggcgag gccaagaccg ctggtgaatg
     1981 cggcgagatc gccgaccttg cctgtcccaa aggcaaagcg cggatcgttg gcgtggcggc
     2041 gcgcatacca ataggcaaaa gccgaaacgc caagcgctgc cgcatgcgtc gacatgtgca
     2101 aaccatcggc gacgagaccc atcgagcgga acgcaagacc gccgatgatc tcgccaaaca
     2161 tcatggccac agtcaacgcg acgaccgcaa gcgtgcgccg ttcgttggcg gcgtgctcag
     2221 cgcctagaaa gacatgcgga tggttcattc cgtcgtttgc atgatcgtgc atgcccgtct
     2281 ctccgttccg cagcgttttc ctcaaggttt aaggaacacg tagatgtcga ctccgagata
     2341 aggatcttcg gcgctggtca aagacgtcaa atattcctcg atgaagaaat tctgcgcctc
     2401 aagacccttc tcgtcgagat aagcagtaat gagatcatac gtcgaatcga tgtcgtcata
     2461 aggacctcga tgctggaact tgatcgcctt gccggctggt gactggccga gcgtgatgcc
     2521 gtcggtcagc gtcgcttttg accctgccgc gatcggcagc atggcggtga agccaaaccc
     2581 attatcgtct tcctgggtga acatcgccag cggatggccg gtgattttga gaccggcctt
     2641 gtcggccgct gccttcaccc ggtccagaat atccttgatc gtcttgaacc cgccatccca
     2701 tctggccttg gcagcgagca ggaccaccgg ccgaggcggc acgtcgaggg tcacggtcgt
     2761 ggaattgttc ggcacgccgg gcgcgccctg atccgcatca ttgggcccaa accccggcgg
     2821 gggccccggt ggcgggggcg gcggttctcc cgaag
//
LOCUS       DASWOG010000481         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_140222,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000481 DASWOG010000000
VERSION     DASWOG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140222"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1672
                     /locus_tag="VGB93_09930"
     CDS             215..1672
                     /locus_tag="VGB93_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0146445.1"
                     /translation="MDEKKMDEKVDQPRRPAPPGDSYAPERKANPALKQNEPAPQARK
                     DAPQAPGDAPVAPAEDIPAAKPRRRSFLGFLIGLIILGGVGYYAYHVLHPAPAERQAH
                     KHAGGPPQSVGVATVGTGDIKIIYAGLGTVTPLATITVRTQINGQLLQVAFKEGDHVK
                     KDDLLAQIDDRPYQLLAQQYEGQLAHDQGLLQQAQLDLVRYQTLVTQNSIARQTAEDQ
                     AYIVKQNQGSVLTDQALIAQEQLNITYCHIVSPVTGRVGIRIVDQGNYVQTSDANGLA
                     VVTQLEPISVIFSLPEDDIPDVMSQMKGSTPLSATVYDRANVHELGTGAVTVLDNQID
                     TTTGMVRFRAEFANADESLFPNQFVNVQVLVRTLQNVITAPVPAIQRGAPGTYVYLVS
                     PNNTVSVQKVTLGQSQGGMVQIISGLKPGDRVVTDGTDRLSDGARVIVPGASAAAESG
                     AAPAGPGTAAPAGEQNTPPAGAAGHHHHHHRQNSE"
     gene            1701..>3346
                     /locus_tag="VGB93_09935"
     CDS             1701..>3346
                     /locus_tag="VGB93_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0146446.1"
                     /translation="MNPSQIFILRPVATTLLTAAILLAGAFSYRFLPLSALPEVDYPT
                     IQIQTFYPGASPEVMTSAVTAPLERQLGEMPGLNQMTSASSAGASVITLQFDLDLSLD
                     VAEQEVQAAINAGTNLLPSDLPAPPIYAKVNPADAPILTIAITSKTLPLTSIEDLVET
                     RLAQKISQQPGVGLVSISGGNRPAIRVQFNPRALAAYGLNIDDIRTTIGNMNVNTPKG
                     SFDGPAQSTTIDANDQITDASQFDKIIVAYRNGNPVRLSDVATTVRGPENTKIAAWAN
                     KTPAILLNIQRQPGANVIQVVDGIKALLPQIEASLPSALNISILSDRTNTIRASVSDV
                     EFELGLAVVLVVLVIFVFLRNLPATIIPSLSVPLSLVGTLVVMYLLGFSLDNLSLMAL
                     TISTGFVVDDAIVMIENIARYIENGEAPLQAALEGSAQIGFTIISLTISLIAVLIPLL
                     FMGEVVGRLFHEFAITLTATIIISAFVSLTLVPMLCAKILRHRPESQRSRWSVAADRG
                     FQWIIDQYGRALRIVLNHQPLTLLVAIATLVVTVLLYITIP"
BASE COUNT          664 a         1103 c          941 g          638 t
ORIGIN      
        1 cgcggattat tgcccttatg ggaaaagagg cgggaaccgg gtaggttggt gatgctttgc
       61 cggaactagt ccccctaggt aaggcatcgc aaggtggccg ttctgatctc ggcggcgcct
      121 ggtttgcaag ccatagatta gggtccgcgc ctctatattg attttaactt gacgatctgc
      181 gccgcgcggg ttgcccatcg ctcggacgaa ccggatggac gaaaagaaga tggacgaaaa
      241 agtcgaccag ccccgtcgac ctgcgccccc cggcgattcg tacgcgccgg agcggaaggc
      301 caatccggca ttaaagcaga acgaacctgc cccgcaggcg cgcaaggatg caccgcaagc
      361 tccaggcgac gcgccggtgg cacctgccga agatattccc gcggcaaagc cacgccggcg
      421 ctcgttcctc ggttttctga tcggcctcat catcctcggc ggcgttggct attacgccta
      481 tcatgtcctc catcccgcgc cggcagagcg ccaggcgcat aagcacgccg gcggcccgcc
      541 gcaatcggtc ggcgtggcga cggtcggcac cggcgatatc aagatcatct atgccggcct
      601 cggcacggtc accccgctgg cgacgatcac ggtccggact cagatcaacg gccaacttct
      661 gcaagtcgcc ttcaaggaag gcgatcatgt caaaaaggac gatctcctcg cgcagatcga
      721 cgaccggcct taccaattgc tggcgcaaca atatgaaggc cagcttgcgc acgatcaggg
      781 gctgctccaa caggcgcaac tcgacctggt gcgctaccag acgctcgtca cgcagaactc
      841 gatcgcgcgg caaacggccg aagatcaggc ctatatcgtc aagcaaaatc agggttcggt
      901 gctgaccgat caggcgctca tcgcgcagga acaattgaac atcacctatt gccatattgt
      961 ctcgccggtg accggccgcg tcggcatccg catcgtcgat cagggcaatt atgtgcagac
     1021 gtccgacgcc aacggcctcg ccgtcgtgac tcagctggag ccgatctcgg tcatcttctc
     1081 gctacccgaa gacgatattc ccgacgtcat gtcgcagatg aaggggagca caccgctctc
     1141 ggcgacggtc tacgatcgcg ccaatgtaca tgaattggga accggcgcgg tcaccgtcct
     1201 cgataaccag atcgatacga cgaccggcat ggtacggttc cgcgccgaat tcgcgaacgc
     1261 agacgagtcg ctcttcccca atcagttcgt caacgttcag gttctcgtgc ggacgctgca
     1321 aaacgtcatc accgcacctg tgccggcgat ccagcgcggc gcacccggca cctatgtcta
     1381 tctcgtcagt ccgaacaaca ccgtctccgt acagaaggtg acgcttggtc aaagccaggg
     1441 tggcatggtc cagatcattt cgggactcaa acccggtgac cgcgtcgtca ctgacgggac
     1501 cgaccggctg agcgacggcg ctcgcgtcat cgttcccgga gcgtcggccg cggcggagag
     1561 cggagcagcc ccagccgggc ctggcacggc ggctcccgcc ggcgagcaga acacgcctcc
     1621 ggcgggcgcg gctggccacc atcatcacca tcatcggcag aattccgagt gatccctgat
     1681 cgcgtagcgg agcacagggc atgaatccgt cgcagatatt tatcctgcgg cctgtcgcga
     1741 ccacgctgct gacggctgcg attctgcttg ccggcgcttt ttcgtatcgg tttctcccgc
     1801 tctcggcctt gcctgaggtc gattatccga ccattcaaat tcagacgttc tatccgggcg
     1861 ccagtccgga ggtgatgacc tcggcggtca ccgcgccgct cgagcgtcag ctcggcgaaa
     1921 tgccgggcct caaccagatg acctcggcaa gttccgccgg cgcgtccgtg atcaccctgc
     1981 agttcgatct cgatctcagc ctcgatgtcg cggagcagga agtgcaagcg gcgatcaacg
     2041 ccggcacgaa tctcctgccg agcgatctgc cggcgccgcc catctacgcc aaggtcaatc
     2101 ccgccgacgc accgattctc acgatcgcca tcacgtcaaa aacgctgccg ctgacctcga
     2161 tagaagatct cgtcgaaacg cggcttgcgc agaagatctc ccaacagccg ggcgtcggcc
     2221 tcgtcagcat cagcggcggt aacaggccgg cgatccgcgt tcagttcaat cctcgcgcac
     2281 tggccgcata cggtctcaac atcgacgata tccgcacgac aatcggcaat atgaacgtca
     2341 atacgccgaa aggcagtttt gacggcccgg cgcaatcgac gaccatcgac gccaacgacc
     2401 agatcaccga tgcgagccaa ttcgacaaaa tcattgtcgc ctatcgcaac gggaaccccg
     2461 tccgattgtc ggacgtcgcg acgacggttc gcggcccgga aaataccaag attgcggcct
     2521 gggccaataa aacgcctgcg atcctgctca acattcagcg acagcccggg gcgaacgtca
     2581 tccaggtcgt cgacggtatc aaggccttgc tgccgcagat agaggcgagc ctgccgagcg
     2641 cactcaacat ttcgatcttg agcgaccgca cgaatacgat ccgcgcgtca gtgtccgatg
     2701 tcgagttcga actcggactc gccgtcgtgc tcgtcgtgct cgtcattttt gtcttcctgc
     2761 gcaatttgcc ggcgacgatc atcccgagcc tttccgtgcc gctctcgctc gtcggcacgc
     2821 tggtcgtgat gtatctcctc ggcttcagcc tcgacaatct ctcgctgatg gcgctaacca
     2881 tctcgaccgg ctttgtcgtc gacgacgcga tcgtgatgat cgagaatatc gcccgctata
     2941 tcgaaaacgg cgaggcgccg ttgcaggctg cgctcgaagg ttcggcgcag atcggcttca
     3001 cgatcatttc gctcaccata tcgctgatcg cggtgctcat cccgctgctc ttcatgggcg
     3061 aagtcgtggg ccgactattt cacgaatttg ccattacgct gaccgcgacc atcatcatct
     3121 cggccttcgt gtcgctgacg ctcgtcccca tgctctgcgc gaaaatcttg cggcatcgcc
     3181 cggagtcaca gcgcagccgg tggtccgtcg ccgccgatcg tggctttcaa tggattatcg
     3241 atcaatacgg gcgggcgctt cgcatcgtcc tcaaccatca gccgctgaca ctgctcgtcg
     3301 ccatcgccac attggtcgtg acggtcctcc tttatattac aatccc
//
LOCUS       DASWOG010000482         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_305676,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000482 DASWOG010000000
VERSION     DASWOG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305676"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VGB93_09940"
     CDS             complement(<1..1316)
                     /locus_tag="VGB93_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146447.1"
                     /translation="MIKAFPGKSGDSSKSAQGQPPARGSPPVLPDETGGRVYGAELNR
                     VEAKLNSLLAAVTAAAEAQPVNWAVQAPAPRRPSLGTTISEISRRQQELDALRAAPSR
                     DSLTAQMDPQPYDTRVRATSLVTLQNDIAGLSAKLDAMQREQTARYAAPPVCNLDKLR
                     GEIAAMSVALRDVASRGSVATVEAAIRGLSQKIEESRNGGTRDSILRPMEELVAELRQ
                     SLAEIDPRMTIKGLESEVSKLGLKIDDLGRAGVDPTAFGHIQAQTREIRDLLSAAIAR
                     PLPVERIERQVAELAQSLGGQRAAPPRENLANVRGAEPAQFSIIEARLEQIAAKVEES
                     LNAAHDQSRYHDLSSRIDTVHEGLSARIADVQRTAPDTSALEDLVRSLSDKIERAVAP
                     QADHHAIEALEEQITLLAQRMEASNPALSLNALERTIGELFGEFERT"
BASE COUNT          281 a          477 c          462 g          332 t
ORIGIN      
        1 gtgcgctcaa attctccgaa gagttcgccg atcgtacgct ccaacgcatt cagcgaaagc
       61 gccggatttg aagcctccat gcgctgggcg agcagagtga tctgctcttc cagcgcttcg
      121 atcgcatgat ggtccgcctg cggcgctacg gcgcgctcga ttttgtcgga cagcgaacgc
      181 accagatctt cgagtgcgct cgtatcgggc gccgtgcgct gcacgtcggc aatgcgcgcg
      241 gacaggcctt catgaacagt gtcaatccga ctagaaagat cgtgatagcg gctctgatcg
      301 tgcgccgcgt tcagcgattc ctcgaccttc gccgcgatct gctcgagcct cgcttcaata
      361 atggaaaatt gcgccggctc cgcgccgcgc acattggcca aattttcccg cggcggtgcc
      421 gctcgctggc cgccgaggct ttgggcaagt tcggcaacct gacgctcgat tcgttcgacc
      481 ggcagtggcc gggcgatagc cgcggagaga agatcgcgaa tttcacgcgt ttgcgcctga
      541 atatgaccga atgcagtggg atcaacgccg gcacggccaa ggtcgtcaat cttcaagccg
      601 agcttgctca cctcgctttc gaggcccttg atcgtcatcc gcggatcgat ctccgcgagc
      661 gactggcgca attcggcgac aagttcttcc atcggccgca aaatcgagtc cctggtgccg
      721 ccattgcgcg attcttcgat cttttgcgac aggcctcgga tcgcagcttc gacagtcgcc
      781 accgagccgc gcgacgcgac atcgcgcaaa gctaccgaca tggcggcgat ctcgccgcgt
      841 aacttgtcga ggttgcagac gggcggcgcg gcatagcgcg cagtctgctc acgctgcatg
      901 gcgtcgagtt tggccgaaag cccggcgata tcattctgaa gcgtgaccag cgacgttgcg
      961 cgtacgcggg tgtcgtaggg ttgtgggtcc atctgcgcgg tcagtgagtc gcgcgacggc
     1021 gcggctcgca aggcgtccaa ctcttgctgg cgccgcgaaa tttcagagat cgtcgtgccc
     1081 agcgaaggcc gccggggggc cggagcctga accgcccagt tcacaggctg cgcttcggca
     1141 gcggctgtca cggccgcgag caggctgtta agctttgctt cgacgcggtt cagttcggcg
     1201 ccatagacgc ggccaccggt ctcatccggc aaaaccggcg gactgccccg cgcggggggc
     1261 tgtccctgcg ccgacttgga agaatccccg cttttccccg gaaaagcttt gatcatgttc
     1321 gctccggagg gaccgcttag cctgtccttt tcccccactt tttcaagtca aatggcatcg
     1381 aatcaaactt ttcgctgcgt cgcgttctct ccacgaattt cggtaactgc ggtagacaac
     1441 gggttaatgt tgatagatta tgagacagct cgacgctttt agattgcatt taataacgag
     1501 tacaatttta agttgtgcaa cgccgtgatt cagtccacct ttgtctgcgc tc
//
LOCUS       DASWOG010000483         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_11876,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000483 DASWOG010000000
VERSION     DASWOG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11876"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGB93_09945"
     CDS             <1..449
                     /locus_tag="VGB93_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2280 domain-containing protein"
                     /protein_id="HEY0146448.1"
                     /translation="FVVQSLAMFDSPNTVAEAVKKEFGIQITRQSIEHYDPTSRQGHD
                     LAQKWADLFEATRKEFLDDTKAIAISHRSIRIRALQRMVAKAEEMRNIALAAQLYEQA
                     AKEMGGLYTNRRELTGRSGGPIETVSTQMTAKEAADAYAAALDGTG"
     gene            415..1257
                     /locus_tag="VGB93_09950"
     CDS             415..1257
                     /locus_tag="VGB93_09950"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY0146449.1"
                     /translation="MPMPQPSTVQAEAWPPNYVDVFAWRQQQVLRFRGNPALIRGAFE
                     YYRTRPAEFINHWVDTYDPRNAAGEVAARIPLILFPRQADLVDFITAAGITSPDYSDI
                     LAELQDGYYSIYGSDAVLTSDSQDGQLLAIFAQAIYDAGQSVIAAYNSFSPANAQGAG
                     LSSIVKINGISRKVPSNSQCVVTVIGQAGAQITNGVVGDNLNLGTQWALPALVTIPPG
                     GSVDVTATCTAAGSTAAAAGSLTQIITPTFGWQSVTNAGGATPGQPVEDDPRCGNDSR
                     NPPR"
     gene            1467..1724
                     /locus_tag="VGB93_09955"
     CDS             1467..1724
                     /locus_tag="VGB93_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146450.1"
                     /translation="MDEPRRVDVVYTGGHWFADVRQIMKPIDRSNLAELIKAVQRSQP
                     GDALLFVVDQSTVEGDEEAEWQLLHAAEKSGALVISSVQEF"
BASE COUNT          381 a          564 c          563 g          366 t
ORIGIN      
        1 cctttgtcgt tcagtcgctc gcgatgttcg acagcccgaa tacggttgcc gaagcggtca
       61 aaaaagaatt cgggattcaa atcactcggc agtcgatcga gcattacgat ccgacatcgc
      121 gccaaggcca tgatctggcg cagaaatggg cagatctatt cgaggcgacg cgcaaagaat
      181 tcctcgacga taccaaggcc atcgcgatct cgcatcgttc catccgcatc agggcgttgc
      241 agcgaatggt ggccaaggcc gaagagatgc gcaacatcgc actagctgca cagctttatg
      301 agcaggccgc gaaagagatg ggcggcctct ataccaatcg gcgggagctt acgggccgca
      361 gcggaggacc gatcgaaacc gtcagcactc agatgaccgc gaaagaggcg gcggatgcct
      421 atgccgcagc cctcgacggt acaggctgag gcgtggccgc caaactatgt cgatgtcttc
      481 gcctggcgtc agcagcaggt tttgcggttc cgcggcaatc cggcgctgat ccgaggcgcc
      541 ttcgaatatt atcggacgcg gccggctgag ttcatcaatc attgggtcga tacttacgat
      601 ccgcgtaacg cagcgggcga ggttgcggcg cgcatcccgc tgatcctgtt cccgcggcag
      661 gcagatctcg tcgatttcat caccgccgcc ggtatcacgt cgccggatta ttccgatatc
      721 ctcgccgaac ttcaggacgg ctattattcg atatatggct ctgatgccgt cctgacctcg
      781 gatagccagg acggccagct cctcgcgatc ttcgcccaag caatctacga cgccgggcaa
      841 tccgtcattg cggcctacaa ttcgttctcg ccagccaatg cgcaaggtgc cgggctatcc
      901 agcatcgtca agatcaacgg catctcgcgc aaagtcccga gcaatagtca gtgcgtcgtc
      961 actgtcatcg gtcaggccgg cgcgcagatc acgaacggtg ttgtcggcga taatctcaat
     1021 ttgggcacgc aatgggcgct tcccgcgctc gtcaccatcc ctccgggggg ctccgtcgat
     1081 gtgaccgcaa cctgcacggc tgcgggctcg accgcggcgg cggcagggtc gctcacccag
     1141 atcatcaccc cgacgtttgg ctggcagtcc gtcaccaatg ctggtggcgc tacgcccggt
     1201 cagccggtag aagatgaccc acgctgcggc aacgacagtc gcaatccacc tcgctgaacg
     1261 ccgagacagt cctcgaggct atttaagccg tggctttatg agcagctccg cacagccgga
     1321 agtgcttgcg cgctgggccc gctgggatgc gatcgaacgc ggtgatgagc cgtggcccga
     1381 ggacggccgg ccaaggcggt aggtgccgga tggcacggca ccgagcattt gatgcaacgt
     1441 tctcaaggct accgtattgt tccggcatgg atgagccacg cagagtcgat gtcgtctaca
     1501 ccggcggcca ctggttcgcg gatgtgcgcc agatcatgaa gcctatcgat cgcagcaatc
     1561 ttgccgaatt gattaaggcg gtgcaacggt cgcagccggg cgatgcgctg ctatttgtcg
     1621 tcgatcaatc gacggttgag ggcgacgagg aggctgaatg gcagcttctg catgcggctg
     1681 agaagagcgg cgcgctggtg atctcgtccg ttcaggagtt ttaggcgtca tcgacatcga
     1741 tgctcgccag atattcgtcg aacttgacca gaacgcgcca tagcgaaagc cgccctcata
     1801 cctagcctca cggcattgtg cgccgccgat gaggaacttg aggcattcga gttccttaat
     1861 ctctcgtcag acgt
//
LOCUS       DASWOG010000484         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_47815,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000484 DASWOG010000000
VERSION     DASWOG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47815"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VGB93_09960"
     CDS             <1..722
                     /locus_tag="VGB93_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146451.1"
                     /translation="PDFGLSIGSVGYTLHRNPKAEVWSNIETEAKFAFFQDPTHEFIM
                     SAAVSAEIGNTGSPQSASLPSDPFSTITPKFFIGKGFGDASADWVRPFAVTGEVDYSI
                     PTVSINSDGSYNPTVLTYGASLQYSLLYENSFVHQLPDVFNRLIPAFEGIFSTPVANL
                     GPADPDDFSTRDTTGVVGPSLYYIGQYFEIGVMAQVPINRASGSHVGALAVLDIFLDD
                     VAPTSLGKPLFSAPLSPLAHY"
     gene            734..1096
                     /gene="copC"
                     /locus_tag="VGB93_09965"
     CDS             734..1096
                     /gene="copC"
                     /locus_tag="VGB93_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEY0146452.1"
                     /translation="MKRIFSLFAISTALMLLDGQAFAHAFLDHAVPGVGTTVAGSPAE
                     LKLTFTEDIVPAFSGVHVATEGGAAIAAAKAVPGPANTLHVRLAHALKPGTYVVTWHV
                     VSVDTHHTQGSYKFTIAP"
     gene            1158..2060
                     /locus_tag="VGB93_09970"
     CDS             1158..2060
                     /locus_tag="VGB93_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0146453.1"
                     /translation="MTILQTLVAARWVHFAALFFLFGCPLSFLVVCGVNKTNGARIGN
                     ALHGLLPIVAVAAAISGLVWIAALIANMAGSFAEAATRETLQAFFFETQFGPIVIVRL
                     ALLAVAMAVLLLPRDIRFATWLVVSAALLVNQAWLGHAANGGASLFGASMIGAYAIHV
                     LAAAAWVGGLPVLLFALFSGRGAPRANDNAAILSRYSLLATGAVTLILLSGIANALFR
                     VHGHPGWLYGTGYGDILAIKLLLVALMLGLAFHNRFIAMPRLGAATPEPLRRCLGLSI
                     SVELLLGVLVIGAAALLGVTPPPD"
     gene            2160..2489
                     /locus_tag="VGB93_09975"
     CDS             2160..2489
                     /locus_tag="VGB93_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY0146454.1"
                     /translation="MSKSIEIAVPHNLGIEEARRRVATEIEQLRSEYVNKFAYSEIAW
                     VGDTANIRVVALAQEVKARIDVLADSVHIEIILPWLLARLAAPLQNKLAATTKDTLSL
                     GFSPKKS"
BASE COUNT          483 a          865 c          763 g          593 t
ORIGIN      
        1 cgcctgattt cggcctctcc atcggctccg tcggctatac tttgcatcgc aatccgaagg
       61 cagaagtctg gtcgaacatc gagaccgaag cgaaattcgc gttcttccaa gacccgacgc
      121 atgagttcat catgtcggcc gcggtaagcg ccgagatcgg caatacggga agtccgcaaa
      181 gcgcctcgct tccctctgat ccattttcga cgatcacgcc gaaattcttc atcggcaagg
      241 ggttcggcga cgcgtctgcc gattgggtgc ggcccttcgc tgtcacgggt gaagtcgatt
      301 attcgatccc gaccgtcagc atcaattccg atggcagcta taacccgacg gtgctgacct
      361 atggcgcatc actgcaatac agtctgctct acgaaaattc gttcgtgcat caattgccgg
      421 atgtcttcaa tcggctgatc cccgccttcg agggcatttt cagcacgccc gtagccaatc
      481 tcggccccgc cgaccccgac gacttttcca cccgcgatac cacgggtgtc gtcggcccct
      541 cgctctatta catcggccaa tatttcgaga tcggcgtcat ggcgcaggtt ccgatcaatc
      601 gcgccagcgg ttcgcatgtc ggcgctctgg ccgttctcga catcttcctc gatgacgtgg
      661 cgccaaccag tctcggcaaa ccgctgttca gcgcgccctt gtctcccctc gcgcattatt
      721 gaaggacatg atcatgaagc ggatattttc tctcttcgcc atcagcactg ctttgatgct
      781 gcttgacggc caggcgtttg cgcatgcgtt tctcgatcat gccgtgccgg gcgtcggcac
      841 aacggtcgcc gggtcgcctg ccgaactgaa gctaaccttc accgaggaca ttgtgccggc
      901 cttctccggg gtgcatgtcg cgaccgaagg tggcgcagca atagccgccg ccaaagctgt
      961 tcccggcccg gcgaatacgc tgcatgtgag gctcgcccac gctctcaaac ccggcactta
     1021 cgtcgtcacc tggcacgtcg tttcggtcga tacgcaccat acacagggat catacaaatt
     1081 cactatcgcg ccatgaacga agccgcgcgc cggattgctg atgccggtgc gcggcccttt
     1141 gccaagcgga cgctctcgtg acaattctgc aaactctcgt cgccgcgcgc tgggtccatt
     1201 tcgctgcgct gttcttcctc ttcggctgcc cgctgtcgtt tcttgtggtg tgcggcgtga
     1261 acaagaccaa tggggcgcgg atcggcaacg cactccacgg attgctgccg attgtggctg
     1321 tggcggcggc catctccggc cttgtctgga ttgcggcgct gatcgccaat atggctggca
     1381 gctttgcaga ggccgccacg cgcgagacgc tgcaagcgtt cttcttcgag acccagtttg
     1441 gcccgatcgt gatcgtccgg ctggctctgc tcgccgtggc aatggcggtt ctcctgctgc
     1501 cccgtgacat ccgtttcgcc acctggctcg tcgtgagcgc cgcgctgctg gtcaaccagg
     1561 catggctcgg ccatgccgcc aacggtggcg ccagcctttt tggcgcatcg atgatcggcg
     1621 cctatgcaat ccacgttctc gcagccgccg cgtgggtcgg cggtttgccg gtgctactgt
     1681 tcgcgctttt cagcggccgg ggcgcgcctc gcgccaacga taatgccgcg atcctgtcgc
     1741 gctattcgct ccttgcgact ggcgccgtca cgctcatcct tctgagcggc atcgccaacg
     1801 ctctatttcg ggtgcatggc catccggggt ggctctacgg cactggctat ggcgacatcc
     1861 tcgccatcaa gctcctgctt gtcgcgctca tgctaggctt ggcctttcac aatcgtttta
     1921 tcgcgatgcc gcggctcggt gcggccacgc cagagcctct ccggcgctgc cttggcctga
     1981 gcatcagcgt cgaactgctg ctgggcgtgc tcgtgatcgg cgcagcggca ctcctcggcg
     2041 tcacgccacc gccggattag cagcataatg gagtcttgct ttcgatccac gagcaaggac
     2101 agacttgaga aggcacagac ttgcccggaa acgcggctcg cagaggcgga aggaccaaga
     2161 tgtcgaaatc gatcgagatt gctgtgccgc acaacctcgg catcgaggaa gcccgacgtc
     2221 gtgtcgccac cgaaatcgag cagctccgga gcgaatacgt caataaattc gcttattcgg
     2281 aaattgcctg ggtcggcgac accgccaata tccgcgtcgt ggccctcgcc caggaggtga
     2341 aggcacgcat cgacgtgctg gccgatagcg tgcacatcga gattattctt ccctggctcc
     2401 tggcaaggct cgccgcgccg ttgcagaata agctcgccgc gacgacgaaa gacaccttgt
     2461 cgctcggctt ctcgccgaag aaatcctgag cctcattcct cccgcgcaag caggagcctg
     2521 caaacaacgg gcaatgaact ccttgtgatt atgcctttaa accattgcca caaattaata
     2581 tttccaggtc gcataaagag cgtgcgctca cgcacggaca aattgttcat tcggagttca
     2641 agtattgtgg gttagcaatg ttagctacgg ggtccgctga agggcgcagg ctgtaacggg
     2701 tcat
//
LOCUS       DASWOG010000485         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_157963,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000485 DASWOG010000000
VERSION     DASWOG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157963"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="serS"
                     /locus_tag="VGB93_09980"
     CDS             <1..732
                     /gene="serS"
                     /locus_tag="VGB93_09980"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0146455.1"
                     /translation="DVKGRGLDERYARNQVLRDARDDLFAQFDLKYWNEHLTKRTLWL
                     IPTAEVPLTNLVREKILDEKDLPIRVTASTPCFRAEAGAAGRDTRGMIRQHQFPKVEL
                     VSVTTPEQSLAEHERLLNCAEEVLKRLGLAYRVVTLCTGDMGFASQKTYDIEVWLPGQ
                     GRYREISSCSLCGDFQARRMNARFRPQDEKATRFVHTLNGSGVAVGRALVAILENYQN
                     EDGSVNVPDVLLPYMGGIKRISKST"
     gene            749..1549
                     /gene="surE"
                     /locus_tag="VGB93_09985"
     CDS             749..1549
                     /gene="surE"
                     /locus_tag="VGB93_09985"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0146456.1"
                     /translation="MRILVTNDDGIHAPGLQVLERIAAQLSDDVIVVAPEHDQSGVSH
                     SLSLNDPLRLRKISERRFAVKGTPTDCVIMGVREILRTRQPDLVLSGVNRGSNVADDV
                     TYSGTIAAAIEGTILGIRSIALSQTYHPSDHDELDWDCAEVHAPRIIESLLAEGIDRD
                     VLINLNFPGCPAGDVEGVSVTLQGRRDMQLARLERRHDGRGNPYYWVAFDRDASARRP
                     GTDLDALWNNRISVTPLKLDMTDEVSVTRYTRVFDKPEFIPRPKERAE"
     gene            1699..2367
                     /locus_tag="VGB93_09990"
     CDS             1699..2367
                     /locus_tag="VGB93_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY0146457.1"
                     /translation="MEENAAAARAEEKAAFLLRLRSGGIRDLDLLRALEKVPREMFVP
                     HRFVDLARRDLVLPIGCGQTLSEPSLVARMIAALGVGPDHRVLEIGTGSGYATAILAE
                     LAAQVMSLERFQTLALAARLRLERLGKQNAEIVFTDGLALPASAGPFDRILIHARLES
                     LPPSLAALLSVEGRIVMARPHARLPHRQMLVELRHGEGGEMQETELGMCRLQALLPGV
                     AEVL"
BASE COUNT          447 a          715 c          748 g          525 t
ORIGIN      
        1 gacgtgaaag gccgcggcct tgacgagcgc tatgcccgaa atcaggtgct gcgcgatgcg
       61 agagatgatc tttttgctca attcgatctg aaatattgga acgaacatct caccaagcgg
      121 acgctttggc tcatccccac cgcagaagtg ccgctgacca atctggtgcg tgaaaaaatc
      181 ctcgacgaaa aagacctgcc gatccgcgtc accgcctcaa cgccctgctt ccgggccgag
      241 gccggggccg ccggacgcga tacgcgcggc atgatccgcc agcatcagtt tcccaaggtc
      301 gaactcgtct cggttacgac accggagcaa tcccttgccg agcacgagcg tctgctgaat
      361 tgtgctgaag aagtgttgaa acggcttggc ctcgcttatc gcgtcgttac gctctgcacc
      421 ggcgatatgg gctttgcctc gcagaagacc tatgacatcg aggtctggct gccggggcag
      481 gggcgttatc gcgagatttc ttcctgctcg ctctgcggcg actttcaggc gcggcgcatg
      541 aatgcccgtt tccggccgca ggacgagaag gcaacgcgtt tcgtccatac cttgaatggc
      601 tccggcgtcg cagtcgggcg cgcgctcgtc gcgattctgg aaaactatca gaacgaagac
      661 ggatccgtga acgtgcccga tgttttgctg ccatatatgg gcggtatcaa gcggatctcg
      721 aagtcaactt aggcggatcg cggtcaagat gcgcattttg gtcaccaatg acgacggcat
      781 tcatgcgccg gggcttcagg tccttgagcg aattgccgcg cagctctccg acgatgtcat
      841 cgtcgtcgcg ccggagcatg accaaagcgg cgtctcgcat tcgctctcgc tcaacgatcc
      901 gctgcggctg cgcaagattt ccgagcggcg tttcgcggtc aaaggcacac cgacggattg
      961 cgtcatcatg ggcgtgcgcg agattttgcg gacgcgtcag ccggacctcg ttctttccgg
     1021 cgttaaccgc ggctcgaatg tcgccgacga cgtaacatat tccggcacga tcgcggcagc
     1081 gatcgaaggt acgattctcg gtattcgttc catcgcgctg tcgcagacct atcatccaag
     1141 cgatcacgac gaattggatt gggattgtgc cgaagttcat gcgccgcgga tcattgaatc
     1201 ccttcttgct gagggcatcg accgcgacgt tttgatcaac ctgaattttc cgggctgtcc
     1261 ggccggagat gtcgagggcg tctcggtcac cctgcaaggg cggcgtgaca tgcagttggc
     1321 tcggcttgag cgtcgccacg atgggcgcgg caacccctat tattgggttg ccttcgaccg
     1381 ggacgccagc gcgcgccggc ccggcaccga tctcgatgcg ctttggaaca accgcatttc
     1441 cgtgacgccg cttaagctcg atatgacgga cgaagtgtcg gtcacgcgct acacgcgtgt
     1501 cttcgacaag cccgaattta ttccacgtcc caaggaacgc gccgaatagc tgttggtgct
     1561 ccggcggcca gctttaagct tggcctaaat catgaaagca tgagccttcg tctgattcgg
     1621 ctgcgtcgcc agcgcggcga gcatggctgc gtcgcgtgag gaacgcagat cttgggcaaa
     1681 agcagatcat tggccattgt ggaggaaaat gccgccgccg cgcgtgccga agaaaaggcc
     1741 gcctttctgc tgcgtttgcg ctccggcggc atccgcgatc tcgatctctt gcgagcgctc
     1801 gaaaaagtgc cgcgcgaaat gttcgtgccg caccggtttg tcgatctcgc gcggcgcgat
     1861 ctcgttttac cgatcggttg cggtcaaacc ctgtccgagc cttcgctggt tgcgcggatg
     1921 atcgcagcgc ttggcgtcgg gcctgaccat cgcgttctcg aaatcggcac tggctccggc
     1981 tatgcgacgg caatcctcgc tgaattggct gcgcaggtca tgtcgcttga gcggtttcag
     2041 acattggccc tggcggcccg gctgcggctc gaacgcttgg gcaaacagaa cgccgaaatt
     2101 gtcttcaccg atgggctcgc gcttccggcg tcggcgggtc ccttcgatcg cattttgata
     2161 catgcacggc tcgaaagtct tccgccgtcg ctcgccgcac ttttgtcggt ggagggccgg
     2221 attgtcatgg cgcgccctca tgcgcgcctt ccgcatcggc agatgttggt cgagttgcgc
     2281 cacggcgagg gtggcgaaat gcaagagaca gaactcggca tgtgccgctt gcaggccttg
     2341 ttgccgggcg tggccgaggt tctttgaggg cggcgcgcgt taagtttacg ttaagctaaa
     2401 atgagaggga attaggtgaa gcggcgttca tctta
//
LOCUS       DASWOG010000486         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_176544,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000486 DASWOG010000000
VERSION     DASWOG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176544"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..578)
                     /locus_tag="VGB93_09995"
     CDS             complement(123..578)
                     /locus_tag="VGB93_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146458.1"
                     /translation="MLFLIREPLWLSGLLLVGLTSMLACLGPSLVRRFVVLEKLTPNN
                     EVAGLEFAAVGRLYAVLLAFSIILVWQKYLTADGTVEKEADAAESMYHLSSGLDEPQA
                     SALRSALTFYLRSAVSDEWPAMEDSDGPQRHHLAAEGFIRPRTGELGLL"
     gene            875..2005
                     /locus_tag="VGB93_10000"
     CDS             875..2005
                     /locus_tag="VGB93_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0146459.1"
                     /translation="MTSSDPEIKVASDDPSKTKPLDGEALPAGANALVPAQPATLVPV
                     DAPPVRKRSRWRLIAIALAALGAAGGGIYWWHVRPAGLPAGIVSGNGRIEAQEIDIDT
                     KFAERVAQLLVDEGDYVVPGQVVARMDTRDVEASLKKAQAQVEEAKRSLDEAHANVAQ
                     QQTEVVFAQQEFDRTKALVERGFATYELLDQRQQTLNGANDALSAANDRVGEAERARD
                     ASTHDVELYKVNIADDTLVSPTSGRIQYRISNVGEVLPAGGKVFTMLDFTDVYMDIYL
                     PTVEAGRARIGADARIVLDSYPDRMIPAHVSFVATQAQFTPKAVETEDERDKLMFRVK
                     VRVDPVFLLSRAALVRTGLPGRAFVRVDRKTIWPASLPGPAQ"
     gene            2002..4821
                     /gene="rbbA"
                     /locus_tag="VGB93_10005"
     CDS             2002..4821
                     /gene="rbbA"
                     /locus_tag="VGB93_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEY0146460.1"
                     /translation="MISGAEVGRICGVSLRYGKTIALDDVTLSIPKGRMVGLIGPDGV
                     GKSSLLALLTGARRIQSGKVYVLGGDLGDARHRAAVCPQIAYMPQGLGKNLYPDLSVR
                     ENIEFFGRLFGQSRSERDSRIAALLKATGLDPFPDRLASQLSGGMRQKLGLCCALIHD
                     PDLLILDEPTTGVDPLSRRQFWSLISRMRAEREGMSVIVATAYMEEAEQFDWLIAMNA
                     GRVLATGSPSSLKEKMGSQTLEESFIALLPEETRRGHKAFSIPPRVADDKMPVIVARN
                     LTRRFGTFTAVNNVSFAIKRGEIFGFLGSNGCGKTTTMKMLTGLLPASAGEVLLFGKP
                     IDASDMRARFRVGYMSQSFSLYTELTVRQNLSLHAHLFHLGKHAHERIAALVARFGLA
                     PYLDQHAADLPLGIRQRLSLAVAIVHKPEILILDEPTSGVDPLARDAFWDLLADLSRN
                     DGMTIFISTHFMNEAARCDHIALMNAGRVLAMGTPAELIKARRSSNLEDAFISYLEEA
                     NETRASGQGSTDAPVQAKAALPIANTRGPTKIWFSPRRLFAYAIREGLELLRDPIRMG
                     FALLGTAFLMAVFGAGISTDVNNLSFAVLDRDNSHESRAYLEELRGSSYFIEKPPIAT
                     PADMEQRLRSGDIKAAIEIPPDFGRDIKKNVPTEDGAWVDGAMPFRAETIRGYLQAAH
                     QQFLSDYAVRTGQTADDTPDDMREALRNKDISLFETPDVNPAVQARIETRFLYNQDFE
                     SIYAQVPGSIALLLVIVPAILMALSIVREKELGSITNLYVTPVTRIEFLLGKQIPYIM
                     IAMINFGILCLMGIFIFGVPIRGNFLVLLIGALLYVTATTGIGMLISSFCKTQIAALF
                     GAAILTMLPATTFSGMLTPVSSLTGLPHLMGIGFPMTYFLKISVGTFTKALGFQDLSM
                     TLLQVAVFIPILTILSLAFLRKQES"
     gene            4835..>5058
                     /locus_tag="VGB93_10010"
     CDS             4835..>5058
                     /locus_tag="VGB93_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0146461.1"
                     /translation="MNRLSNIFWLGTKELRSFFSDVVLLGLVIYTFSFAIYVQGHSNA
                     QELYNGSVAYVDDDKSELSRRMMAVFLPPY"
BASE COUNT         1051 a         1516 c         1394 g         1097 t
ORIGIN      
        1 ctgaggcatc ccaggcgttt ctgaaaccgg accagccatt gcttggataa ccagcaaggg
       61 ggaatcggct agccgtcagg caagctaaga attcccgctg ggtcatatct aactcaattg
      121 attcagagga gtccgagctc ccccgttcgt ggccggataa atccctcagc cgccaaatga
      181 tgtctttgcg gaccgtcgct atcttccatg gcgggccact cgtcagagac ggcggaccga
      241 agatagaaag ttagagcgct ccgtagcgcg gaggcttggg gttcgtccaa gcctgacgaa
      301 aggtgataca tcgattctgc ggcatccgcc tctttttcga ccgtaccatc ggcggtcaag
      361 tatttctgcc agacgaggat aatcgaaaaa gcaagaagca cggcataaag acgcccaacc
      421 gctgcgaact caagtccagc gacttcattg ttcggcgtta acttttcaag cacgacgaag
      481 cgacgaacaa ggctgggtcc gagacatgcg agcatacttg ttaatccgac cagcaagagg
      541 ccggaaagcc aaagtggctc gcggataaga aacagcatct gttggtcgct cgcgtgaaat
      601 aaatccgctc gccagcatga gcctgaattt gtcgcatacg aatcattaac agcggtttag
      661 cttgtccgcg ccgcaacctc ttgcctgacg gcccggcgct gcgagccaag cccctcggat
      721 tcctgaccgc cgcagcgagc cctttggtag ctcccgattg atcgaaatca agtagtccgc
      781 gccccttcga cgctatttat cagtttagag tgcagtgcac acggctgatc ccgcgtgctc
      841 ttggcaattg taagcaagcc gatggatact aaccgtgacc agctccgacc ccgagatcaa
      901 ggttgcgtcc gacgatccaa gcaaaaccaa acctctcgat ggcgaagccc tcccagcggg
      961 tgcaaatgct ctcgttcctg cccagccggc gacgctagtc ccagtcgatg ccccgccggt
     1021 tcgcaagaga tcgcgctggc gacttatcgc catcgccctg gccgcactgg gcgcagcggg
     1081 cggcgggatt tactggtggc acgtgcgtcc ggcgggtttg ccggcaggta tcgtttccgg
     1141 caatgggcgt attgaggccc aagagatcga tatagacacg aagtttgctg agcgagttgc
     1201 gcagctgctt gtcgacgaag gtgactatgt cgtacctggc caagtcgtcg cacggatgga
     1261 tacgcgtgac gtcgaagctt cgctcaaaaa ggcccaggcg caggtcgagg aggcaaaacg
     1321 cagccttgac gaggctcacg ccaatgtcgc gcagcagcag acggaagtcg tcttcgcgca
     1381 gcaggagttc gatcgaacga aggcgcttgt cgagcgcggt tttgcgactt acgaacttct
     1441 ggatcagcgc caacaaacgc tcaacggcgc aaacgacgcg ttaagcgcgg ccaacgaccg
     1501 cgtgggggaa gccgagcggg cacgcgatgc atccacgcat gatgtcgaac tttataaagt
     1561 gaacatcgcc gacgacactc tggtatcgcc caccagcggg cgcatccaat accgcatttc
     1621 caacgtcggc gaggttctcc cggcgggcgg caaggtgttt acgatgctgg attttacgga
     1681 cgtgtatatg gacatttacc tgccgaccgt ggaagccgga cgcgcacgga tcggcgcgga
     1741 cgcgcgcatc gtgctggatt cctacccgga caggatgatt ccggcgcatg tctcgtttgt
     1801 cgccacacag gcgcaattta cgccgaaggc cgtcgagacc gaagacgaac gcgataagct
     1861 gatgtttcgc gtcaaagttc gcgtcgaccc cgtttttctg ctctcacgtg ccgcgctcgt
     1921 gcgcaccgga ctgccgggca gggccttcgt gcgcgttgac aggaagacca tttggccggc
     1981 ttcgctgccc ggtccggcgc aatgatatct ggcgccgagg tcggcagaat ttgcggagtt
     2041 tcgctgcgtt acggcaagac cattgcgctt gatgacgtga cgctctcgat cccgaaaggc
     2101 cggatggtgg ggctgatcgg tcccgacggc gtcggtaaat cctccctgct cgcgctgctc
     2161 acgggcgccc ggcgcatcca gagcgggaag gtgtatgtcc ttggcggcga tctcggcgac
     2221 gcgcgccatc gcgccgccgt ttgtccgcag atcgcctata tgccgcaagg gctggggaag
     2281 aatctctatc ccgatctcag cgtgcgcgag aacatcgaat tttttggccg cctcttcggt
     2341 caatcgcgca gcgaacgcga ctctcgtatc gccgctcttt tgaaagcgac cgggctcgat
     2401 ccctttccgg atcggctcgc aagccagctc tcaggtggca tgcgccaaaa gcttgggctt
     2461 tgttgcgcgc tgatccatga tcccgatctt ctcattctgg atgagccgac aacgggcgtc
     2521 gacccgctct cgcgacggca attctggagt ctcatttccc gtatgcgcgc cgagcgcgaa
     2581 ggcatgagcg tcattgtcgc gacggcctat atggaagagg ccgagcaatt tgattggctg
     2641 atcgcgatga acgccggccg cgttctcgcc actggatcgc cgtcctctct caaagagaag
     2701 atgggatcgc aaacgctcga agagagcttc atcgcacttc ttccggaaga aacacggcgc
     2761 ggccacaagg ccttttctat tccgccgcgg gtggctgacg acaagatgcc ggtcattgtc
     2821 gcgcgcaacc tgacgcgccg cttcggcaca tttaccgccg tgaataatgt cagcttcgcg
     2881 atcaaacgcg gcgagatttt tggtttcctc gggtcgaatg gttgcggcaa gacgaccacg
     2941 atgaaaatgc tgacgggcct ccttcccgcc agcgccggcg aagtgcttct gtttggcaag
     3001 ccaatcgatg cttcagatat gcgcgcaagg tttcgcgtcg gctatatgtc gcaatccttt
     3061 tcgctttata ccgaattgac cgtaaggcag aatcttagcc ttcacgctca tctgtttcat
     3121 ctcggcaagc acgcccatga gcgcattgcg gcgcttgtcg cccgcttcgg gcttgcccct
     3181 tatctcgatc agcacgctgc cgaccttccg cttggcattc gtcagcgcct ttcgctggcc
     3241 gtcgccatcg tccacaagcc cgaaatcctc atcctcgacg agccgacatc cggcgtcgat
     3301 ccgctggcgc gcgacgcctt ctgggatctc ctggccgacc tttcacgcaa cgatggtatg
     3361 acgatcttca tctcgacaca tttcatgaac gaggctgccc gttgcgatca tatcgcattg
     3421 atgaacgcgg gacgcgttct cgcgatggga acaccggcgg aactgatcaa ggcccgtcga
     3481 tcttctaacc ttgaagacgc cttcatctct tatctcgaag aagcgaacga gacccgcgcc
     3541 tccgggcaag gttcgacgga cgcgcccgtt caagcgaaag cggcgctgcc catagccaat
     3601 acccgcggac ccacgaaaat ctggttcagc ccgcgccgtc tcttcgccta tgcaatccgc
     3661 gaaggactgg aactcctgcg agacccaatc cgcatgggct tcgccctact cggcaccgca
     3721 tttctcatgg cggtcttcgg ggctggcatt tcgacggacg tgaataattt atccttcgct
     3781 gttctcgatc gcgacaattc gcacgaaagc cgcgcttatc tcgaagagct acgcggctcg
     3841 agctatttta tcgaaaagcc gccgatcgcg acccccgccg acatggaaca gcgcctccgc
     3901 tccggggaca taaaggcggc catcgaaatc ccgccggact tcgggcgcga catcaagaag
     3961 aatgtgccaa ctgaagacgg cgcgtgggtg gacggcgcta tgccgtttcg cgccgaaacg
     4021 attcgcggct atctccaggc ggcccatcag caatttctct ccgattacgc ggttcgcacc
     4081 gggcaaactg cggacgatac accggatgat atgcgcgagg ccttgcgcaa taaggatatc
     4141 tcactgttcg agacaccgga tgtaaacccc gccgtgcagg caaggattga aacgagattt
     4201 ctctacaatc aggattttga aagcatctat gcccaggttc ccggcagcat cgctctgctg
     4261 ctcgtcatcg ttcccgccat tttgatggca ctctctatcg tacgtgagaa ggagcttggc
     4321 tctatcacga atctttatgt gacacctgtc acgcgcatcg agttcctgct cggcaaacaa
     4381 atcccctaca tcatgatcgc catgatcaat tttgggattc tctgtctgat gggaattttc
     4441 atattcggtg tcccaatcag gggaaatttc ctggtcctgc ttatcggcgc gttgctctat
     4501 gtgacggcaa cgaccggaat aggaatgctg atatcttcct tttgcaaaac acagatcgcg
     4561 gccctgttcg gcgcggcaat cctcacgatg ttgcccgcca cgacattctc cggaatgctg
     4621 acgcccgtct cctctcttac cggcttgccc catttgatgg gcatcggctt cccgatgacc
     4681 tattttttga agatcagcgt cggcactttc accaaagcgc tcggctttca agacctttcc
     4741 atgacattgc tacaggtcgc cgtcttcatt ccaatcctga cgatcctcag cctggcattt
     4801 ctgcgcaagc aggaaagtta gaaggcaccc tggaatgaac agactcagca atattttctg
     4861 gcttggcacg aaggaacttc gcagcttctt cagtgatgtc gtgcttctcg gtcttgtgat
     4921 ctacacattc tcctttgcga tttatgtaca ggggcatagc aacgcgcagg aattatataa
     4981 tggctccgtc gcctacgtgg acgacgataa atcagaatta tcccgcagga tgatggcggt
     5041 ttttctgccc ccttattt
//
LOCUS       DASWOG010000487         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_30223,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000487 DASWOG010000000
VERSION     DASWOG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30223"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..391
                     /locus_tag="VGB93_10015"
     CDS             116..391
                     /locus_tag="VGB93_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146462.1"
                     /translation="MLYLRSDDSFFRGTDFSDDEIMKRLRDGGAIVIDASLSKEKKDG
                     VQISIPGDGHPTPYANQLRASILKTYLAEHAPDILKSVSDTQCLGAQ"
     gene            complement(407..2245)
                     /locus_tag="VGB93_10020"
     CDS             complement(407..2245)
                     /locus_tag="VGB93_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEY0146463.1"
                     /translation="MRVLGISAFYHDAAAALVEDGRIVAAAQEERFTRKKHDPAFPRH
                     AIEYCLSQTAGGSGVDRIIFYEKPFVKFERLLETYVAFAPQGFASFRKAMPLWIGEKL
                     FQRDLLLRELKAIDPALGDRQKLGFSEHHYSHAASAYYPSPFTSALVLTMDGVGEWAT
                     TSLALGQGDDLNIIKEIRFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGAPKYAQ
                     TILDHLIDVKTDGSFRLNMDYFSYATGLTMTDARFDALFGGPPRTPDQRLDQRHMDLA
                     ASVQAVTEEVVLRLTRAAASETGEKNLCLAGGVALNCVANGKILRDGKFERIFIQPAA
                     GDAGGALGAAFAGYYDGRRDGQRKSLVQEPDAMQGSYLGPAYAQDDIEMRLQNAGAKF
                     DVVDDTTLISETAAVLARGQAVGWHQGRMEFGPRALGGRSILGDPRSPTMQKALNLKV
                     KYRESFRPFAPSVLREHVSEWFDLDSDSPYMLLVANVKETRCRKMSGNEEHLFGIDKL
                     NIMRSEIPAVTHVDYSARVQTVHKETNPRYHALIEKFAELTGCPVLVNTSFNVRGEPI
                     VMTPEDAFACFMGPAIEFLAVGNCILRKDAQNPALKRDYKDAFELD"
     gene            complement(2252..>2383)
                     /locus_tag="VGB93_10025"
     CDS             complement(2252..>2383)
                     /locus_tag="VGB93_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEY0146464.1"
                     /translation="LFEFLGTRKKFWLIPLLVLFLVFGGLFVLTQGSVVAPFIYTLF"
BASE COUNT          508 a          669 c          709 g          497 t
ORIGIN      
        1 tgaattcgtg taaccgagcc gacgccagct gacgcatgac gatatagagc tttatattcg
       61 tatccttgat gcagcggttc aactggcgaa aaagaagtat gacgcatcga ccgtcgtgct
      121 ctatttgcgg tcggatgaca gctttttccg tggcaccgac ttttccgacg acgagatcat
      181 gaagcggttg cgcgatggtg gtgcgatcgt gatcgatgcg agcctttcaa aggagaaaaa
      241 ggacggcgtt cagatcagca taccgggcga tggccacccc acgccctatg caaaccagct
      301 tcgcgcttca attctgaaga catatctcgc tgagcacgct ccggacattc tgaaatcagt
      361 ttccgatacg caatgcttgg gcgcgcaatg agaatgggct agatatttag tccagttcaa
      421 aggcgtcctt gtaatcgcgc ttgagcgccg ggttctgcgc gtccttgcgc agaatgcaat
      481 tgccgacggc gaggaactcg attgccggtc ccatgaagca ggcgaacgca tcctccggcg
      541 tcatgacgat cggctcgccg cgcacattga agctcgtgtt aacgagcacc gggcagccgg
      601 tcagttcggc gaatttctcg atcaaggcgt ggtagcgcgg attggtttct ttgtgcactg
      661 tctggacgcg cgccgaataa tcgacatgcg tcacggccgg aatctccgag cgcataatat
      721 tcagcttgtc gatgccgaac agatgttctt cgttgcctga cattttgcgg cagcgcgtct
      781 ctttgacgtt ggcgacgagc agcatgtagg gcgaatcgct gtcgagatcg aaccattcgc
      841 tgacatgttc ccgcagcacg gagggcgcga acgggcggaa actctcgcga tattttactt
      901 tgaggttcag cgccttctgc atcgttggcg agcgcggatc gccaaggatc gagcggccac
      961 cgagcgcgcg ggggccgaat tccatccggc cctgatgcca gccgacagcc tgacctcgcg
     1021 cgagcaccgc cgccgtttcg gagatcaagg tcgtgtcatc gacgacatcg aatttcgcgc
     1081 cggcattttg cagccgcatc tcgatgtcgt cctgcgcata ggcgggcccg agatagcttc
     1141 cctgcatcgc gtccggttct tgcacgagcg attttcgttg gccgtcgcgg cgtccgtcgt
     1201 aatagccggc aaacgccgca ccgagtgcgc cgcccgcatc gcccgccgcg ggctggatga
     1261 aaatgcgttc gaatttcccg tcgcgaagaa ttttgccgtt ggcgacgcaa ttcaacgcga
     1321 cgccgccagc gaggcagaga tttttttcgc cggtttcgct ggccgccgcc cgcgtcaagc
     1381 gcaacacgac ctcttcggtc acggcctgta ccgaggcagc aagatccata tggcgttgat
     1441 cgagcctttg atcaggcgtg cgcggcggtc cgccaaataa cgcatcgaac cgagcgtcgg
     1501 tcatggtgag gccggtcgcg taggaaaagt aatccatatt caaccggaat gagccgtcgg
     1561 tcttcacgtc gatcagatga tcgaggatcg tctgtgcata tttcggcgca ccatagggcg
     1621 cgaggcccat aactttgtat tcgcccgaat tcaccttgaa gccggtgtaa taggtgaagg
     1681 ccgaataaag caggccgagc gaatgcggaa agcggatttc cttgatgatg ttgagatcgt
     1741 cgccctggcc aagcgcaagg ctcgtcgtcg cccattcacc gacgccgtcc attgtcagaa
     1801 cgagcgccga cgtgaaaggc gacgggtaat aggccgaggc cgcgtgagaa taatgatgtt
     1861 cgctgaagcc gagtttttgc cggtcaccca gcgccggatc gatcgccttt aactcgcgca
     1921 gcaggaggtc gcgctgaaag agtttttcgc cgatccataa cggcattgct ttgcggaacg
     1981 aggcaaaccc ttgcggcgcg aaggcgacat aggtctcgag cagacgctcg aatttcacga
     2041 atggcttttc gtagaagata atgcggtcga cgccactgcc tcccgcggtc tggctcaggc
     2101 aatattcgat cgcgtggcgc ggaaatgccg gatcgtgctt cttgcgggtg aagcgttctt
     2161 cctgcgccgc ggcgacaatg cggccgtctt cgaccagtgc cgcagccgca tcgtgataga
     2221 atgctgaaat cccgagaaca cgcaccggcg attagaacag cgtatagatg aacggtgcaa
     2281 cgacggagcc ttgcgtcaga acgaacaggc cgccgaatac gaggaaaagc acaagcagcg
     2341 ggatcagcca gaatttcttg cgtgttccga ggaattcgaa aag
//
LOCUS       DASWOG010000488         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_11939,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000488 DASWOG010000000
VERSION     DASWOG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11939"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB93_10030"
     CDS             <1..186
                     /locus_tag="VGB93_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146465.1"
                     /translation="VTRALGDHRQEEKAQFAIVEESATAAVPAKATLMTVVMTIRRIV
                     PGCKTVRTILVSLSHGR"
     gene            447..899
                     /locus_tag="VGB93_10035"
     CDS             447..899
                     /locus_tag="VGB93_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0146466.1"
                     /translation="MLTSKAKYGLKALMHLATYDGPCLAEEIATENNIPRKFLDAILV
                     ELRNAGILTSRRGKGGGYHLGRPADKITVGQIIRILDGSLAPIACASRTAYRPCRDCP
                     DENACQIRDLMIDVRDSMALILDRTTIMSLKGRNAREAERLWERAHRP"
     gene            980..1606
                     /locus_tag="VGB93_10040"
     CDS             980..1606
                     /locus_tag="VGB93_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0146467.1"
                     /translation="MLGVGLPANRDDPLRHFSTILGERLRRLMARERISLDKLSDFLG
                     ICRNSLSRIYDGEVVPTISLIWKIANVFGVPFGSLISSREGRGTFVLRSADTAVLSSS
                     DGQFTSRALFPYNSARLVEFYELRIAPGHTEDAVAHVPGTVECLVVTRGTVEITSGKE
                     PSETLRKGDAIVFEADVPHRYANRGKNEAVLYLVMSYARLDDERGHDN"
     gene            complement(1660..2520)
                     /locus_tag="VGB93_10045"
     CDS             complement(1660..2520)
                     /locus_tag="VGB93_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146468.1"
                     /translation="MRIFRELRDLSDAPRGASLVLGSFDGVHRGHAALIAAARARKNP
                     VGVVTFTPHPRLLTQPRQAPFLLTLAEQKYSALRALGADFCVELPFTREFASISAEDF
                     VSAILAGRLRAGHVVCGYNFRFGAARKGDVALLEHLGAQLGFSVGNVGPVLGDDGEAY
                     SSTRIRNCLRSGDIAGATAVLGRPWTIEVRFQREPHARDARGYFKFGEYLKPLPARYN
                     ARVRSENDILVDARLIVSQQNHVGWLELPIHSSRQWEGNIELDLIELIRPIGVRDWRS
                     ETDVERTCYI"
     gene            complement(2609..>3274)
                     /locus_tag="VGB93_10050"
     CDS             complement(2609..>3274)
                     /locus_tag="VGB93_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146469.1"
                     /translation="VGQTGSGKTSVTALVHRFYDVWSGAVLVGGHNVKDITRDSLGRA
                     VAMVLQEPFLFSGSIFENIRYSSVWATRDDVVAAAQAVCAHEFIRKLPEGYDTNLGQR
                     GQNLSMGQRQLLSFARALVADPKILILDEATASIDSFTEQSIQAALRVLLKGRTSIVI
                     AHRLATVRDADNIIVLRNGAIAEQGDHESLLRQGGLYADLWRKNYASFDDQIFGALDV
                     GNR"
BASE COUNT          732 a          959 c          896 g          687 t
ORIGIN      
        1 gtgacgcggg cgctcggcga tcatcgccag gaggagaagg cgcagttcgc catagtcgaa
       61 gagtcggcca cggccgccgt gccggcgaag gccaccctca tgacggtagt gatgacgatc
      121 cgacggatcg tcccgggatg caaaacggtc cggaccatcc ttgtctcttt gtctcatggt
      181 cgctgaccta tatcaagata tagctagatt caagatatat ctagagaata ttcgtttacc
      241 gaattgacat tcagactggc aagcaaacga tcggatcaac cgatctcagg cggccgtgat
      301 tcccggtcag cggtctcgat ttttccgcat cgatttcttt tgcggcgcgg aagaattgcc
      361 tcgggggagc acggcaaaga ggaaattgct ctctttctca aaatcgacta ggcataaagg
      421 attaacgccg ggtcccgctc gtctcgatgc tcacaagtaa agcaaaatac ggcctcaagg
      481 cgctgatgca tctcgcgacc tatgacggtc cgtgtctcgc ggaagagatc gccacggaga
      541 acaatattcc tcgcaaattt ctcgatgcca ttctcgtcga gttgcgcaat gcgggaattc
      601 tcacgagtcg gcgcggcaag ggcggcggtt atcacctcgg cagaccagcc gacaaaatca
      661 cggtcggcca gataattcgc attctcgatg gatctttggc tcccatagct tgtgcgagcc
      721 gcacggccta tcgcccttgt cgcgattgtc cggacgagaa cgcctgccag attcgcgacc
      781 tcatgattga tgtccgcgat tcaatggcgc tcatcctcga tcggacgacc attatgtcgc
      841 tgaaaggccg caacgcgcgt gaagcagagc ggctttggga acgcgcgcat cgtccttgac
      901 ctaaaggcat ctttctctat agtctatagt aattatagat tgtggggtaa cagacgtggc
      961 gggcgggaat agtagaagtg tgctcggagt cgggttaccc gcaaatcgag atgatcctct
     1021 tcgtcatttt tcgacgatcc tcggagagcg ccttcgccgc ctgatggcgc gggaacggat
     1081 ttcactcgac aaactgtcgg atttcctagg catctgccga aatagcctat cgcgcattta
     1141 cgacggcgaa gttgtcccca ccatcagcct gatctggaaa atcgccaacg tctttggtgt
     1201 tccttttggc agcctgatct cttctcgcga aggacgcggc acgtttgttc ttcggtccgc
     1261 cgacacggct gtcttatcgt cgagcgacgg gcaattcacg tcgcgcgcgc tctttcctta
     1321 caacagcgcg cgcctagtcg aattctatga attgcggatc gcgcccggtc ataccgagga
     1381 cgccgtcgct catgttcctg gaaccgtcga atgtctcgtc gtgacgcgcg ggacggtcga
     1441 gataacatcc ggcaaagaac cgtcggagac cttacgaaaa ggcgacgcaa tcgtcttcga
     1501 agccgacgtt ccgcatcgct atgcgaaccg cgggaaaaat gaagctgtgc tttatctggt
     1561 gatgtcctac gcgcgcttgg acgacgaaag aggccacgac aactgaccgg cgcctgcacg
     1621 acagattcga cagccgtacc ccggcgtcgc aaagatgcgt cagatgtaac aagttctttc
     1681 gacgtccgtt tcgctgcgcc aatcccggac accgatcggc ctgatcaact cgatcaggtc
     1741 gagttctata ttcccttccc attgccgcga agaatgtatc ggcaattcga gccacccgac
     1801 atgattctgt tgcgagacga tgagacgtgc atcgacaaga atgtcatttt ccgaccgcac
     1861 ccgcgcattg tagcgcgcag gtaatggctt cagatattcg ccgaatttaa aatagccgcg
     1921 cgcatcacgc gcatgtggct ctctttgaaa acgcacttcg atcgtccatg gccgcccaag
     1981 gacagctgtg gcgccggcga tgtcaccaga tcgaaggcaa ttgcggatgc gggttgatga
     2041 ataggcttct ccatcgtccc ctagcacggg accgacgttc cccacggaga acccgagttg
     2101 tgcgccgagg tgctcgagca atgcgacgtc acctttccgc gcggcgccga agcgaaagtt
     2161 atagccacaa acgacatggc cggcacggag gcggccagcc agaatcgcag agacaaagtc
     2221 ttcagccgag atagatgcaa attcgcgcgt aaacggcagt tcgacacaaa agtccgcacc
     2281 cagcgcgcgt agagcggagt atttctgttc tgcgagcgtc agaagaaagg gcgcctgtcg
     2341 aggctgtgtc agaaggcgcg ggtgcggcgt gaaagtcacg acgccgaccg gatttttacg
     2401 cgcgcgcgcg gcggcgatga gtgccgcatg accgcggtga acgccgtcga aactgccgag
     2461 aaccagcgaa gcgccgcgtg gcgcatcgct caagtctcga agctcacgaa aaatgcgcat
     2521 gccatctcca acaaaactcg cctaagttgc cttccacact gtaagggctc gcaatcagaa
     2581 cttgtcgatc ataccgtcct tgcgcccgct acctattccc gacatcaagc gcaccgaaga
     2641 tttgatcgtc gaatgatgcg taatttttcc gccagagatc ggcgtagaga ccgccttgcc
     2701 gcagcaggct ctcatgatcc ccctgctccg caatggcgcc attccgcagg acgataatat
     2761 tgtcggcatc gcgcaccgtc gcaaggcgat gcgcaatgac gatacttgtt cggcccttca
     2821 acagcacacg cagcgcagct tgaatgcttt gttctgtgaa actatcgatg ctcgcggtcg
     2881 cttcatcgag gatgagtatt ttcggatcgg caacgagggc ccgcgcaaaa ctcagaagtt
     2941 gtctttgacc catcgataga ttctgtcctc gctgcccgag gttcgtgtcg taaccttccg
     3001 gcagcttcct gataaactca tgcgcacaaa cggcctgcgc ggccgccacg acatcgtcgc
     3061 gcgtcgccca cacgctgcta tagcggatgt tctcgaagat cgagcccgag aacaggaatg
     3121 gttcctgcaa gaccatcgcg acggcacggc cgagcgagtc ccgcgttata tctttgacat
     3181 tatggccgcc aaccagcacc gcgccagacc acacatcgta aaaccgatgc accagcgctg
     3241 tgacacttgt cttgccggat cctgtctggc caac
//
LOCUS       DASWOG010000489         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_140371,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000489 DASWOG010000000
VERSION     DASWOG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140371"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGB93_10055"
     CDS             complement(<1..270)
                     /locus_tag="VGB93_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0146470.1"
                     /translation="MASIYDFSAKTLDGNEKSLADFRGQVVLVVNTASKCGFTPQYGG
                     LETLYKDLHARGFEILGFPCNQFGGQEPGNEQDIGQFCTLNYGVTF"
     gene            complement(276..758)
                     /locus_tag="VGB93_10060"
     CDS             complement(276..758)
                     /locus_tag="VGB93_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146471.1"
                     /translation="MPTGFWVPLLAGVFGTLIGSIATITAALINRQPTPASILDSRIV
                     TLMQIYEKTIGELRIEIARLQEKIDIYERTVMDLRDHIAQLEAKIDIMKARMARWPLV
                     RRRWRRSANSQVYWFRPLAPVPVCPGQGPFLIPASRANPLAVPESFALEQSDWRQQDG
                     "
     gene            complement(779..1096)
                     /locus_tag="VGB93_10065"
     CDS             complement(779..1096)
                     /locus_tag="VGB93_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146472.1"
                     /translation="MKYAIYAGVLVLPFYLAGCADLDETLNKAVSWIDAPATQHAIES
                     LKTGVTAFTCAVADVGAVAQQIETGVGGGQSIIGTDGKVYVGSSTVCAALGGTLGPQA
                     VIP"
     gene            complement(1118..1423)
                     /locus_tag="VGB93_10070"
     CDS             complement(1118..1423)
                     /locus_tag="VGB93_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146473.1"
                     /translation="MTSDLSSLATDVQKVATVLKYASQINFSKAAQAFESANLSGEIE
                     TLDDILRVVGVFVPPVAIAANDLAAALGGFELLIQFSNGKIPQLTLDLEQTIQDRFE"
     gene            complement(1420..1920)
                     /locus_tag="VGB93_10075"
     CDS             complement(1420..1920)
                     /locus_tag="VGB93_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146474.1"
                     /translation="MRMRIFCVAAMTLAAPSAHAAGNTLVDLPWGALCNEVLKALQPV
                     AISLAGLALTWLAAKLGPDIARALYAAHIDQVMTRAIDAGFALVEGAEKGKVLEIPVA
                     NEVLRRAAQYPIAQAPGLFKELGDNLGQMLVARLSASGALPPQASPANLDLAPATRIK
                     PERTVA"
     gene            2063..2296
                     /locus_tag="VGB93_10080"
     CDS             2063..2296
                     /locus_tag="VGB93_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146475.1"
                     /translation="MKVKTFSGAMILAMSIIGAPAAQAHHRHMRHTHPREPVQTFSHE
                     TERPDTTQRVDAYVNSEGEYIPSLLEPSAPSDQ"
     gene            complement(2300..2575)
                     /locus_tag="VGB93_10085"
     CDS             complement(2300..2575)
                     /locus_tag="VGB93_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146476.1"
                     /translation="MKKAIAITILSLGLSPLSAEAANFTCHGAGQCVCTQTMSAKSMI
                     GYNATDDQESVNVNITCINMTTMSVAYYTVRKEDGLGGQYNGMVPDH"
     gene            complement(2609..3097)
                     /locus_tag="VGB93_10090"
     CDS             complement(2609..3097)
                     /locus_tag="VGB93_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0146477.1"
                     /translation="MTLHNTGVPSLATWVGPAHPAQQRIVSQKHYERDILYWHSGVHL
                     FVAQDLIWNLCDLTQVGVSVSCWNHLTLGMEMVGDYAAESFESGPGAQVRDNAVAALA
                     VLHRKLGLRPDGFRLGVRGLHFHKECRRDHHDCPGRNVVKADVVARVLAQMAAMEKAA
                     AS"
     gene            complement(3132..3575)
                     /locus_tag="VGB93_10095"
     CDS             complement(3132..3575)
                     /locus_tag="VGB93_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0146478.1"
                     /translation="MPQTRRPIFARADVAAVVAAKAEALDVPARLALAVVRFESGLRM
                     SMHGAAGERGTVQVLPRTARKVGIVGNLYGQAGIEVGVRYLKLAMAMHRQAGWCAVAS
                     AYNSGVGRVAPIAQLTAARWRPWRRCDDVSRRAQGICPGRIRHFR"
     gene            complement(3929..>4697)
                     /locus_tag="VGB93_10100"
     CDS             complement(3929..>4697)
                     /locus_tag="VGB93_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0146479.1"
                     /translation="ADYPGLGTLGPHPYLVGLSEARAVIDSVRVARSMPGAGGGSRFV
                     VWGHSQGGHAALFTGLIAKNYAPELTLLGVAAAAPASELTALMDDDLDTIAGTNITAM
                     TLWSWQRVYGAPMDRVVDPRAMPAIDKLARECIEGPWDISTRNRTTRPLEQHFLTVAH
                     PADVQPWRDLLAKNTPGPLPPDLPVFLAQGLDDAVIRPAVTQDYFDKLCKGGSKARLF
                     LMPGVGHGGAAMHSALEAVAWTSARFAGETPPTDCGK"
BASE COUNT          964 a         1447 c         1403 g          883 t
ORIGIN      
        1 gaaggtcacg ccgtaattga gcgtgcagaa ctggccaatg tcctgttcgt tacccggttc
       61 ctggccacca aattggttgc acgggaaacc cagaatctcg aagcccctag cgtgcaaatc
      121 cttatatagg gtctcaaggc ccccatattg cggggtgaac ccgcatttgc tcgcggtatt
      181 gacgacgagc acgacctgcc cgcggaaatc ggcaagcgat ttctcgttgc cgtccaatgt
      241 cttggccgaa aaatcgtaaa tcgaggccat gctgttcacc cgtcttgctg acgccaatcg
      301 ctttgctcta atgcgaaact ctcgggcact gcaagcggat tggcccggga ggccggaatc
      361 aaaaaaggcc cctgccccgg gcagaccggg acaggggcta aaggacgaaa ccagtaaact
      421 tgactattcg cggagcgccg ccagcggcgc cggaccagcg gccacctcgc cattcgcgcc
      481 ttcataatat cgattttggc ctcgagctgc gcaatgtgat cgcgaagatc catcacggtg
      541 cgttcgtaaa tatcgatctt ctcttgcaga cgggcgatct cgatccgcaa ttcgccaatc
      601 gtcttctcgt agatctgcat caaggtcacg atgcgggaat cgaggatcga agcgggcgtc
      661 ggctggcggt tgatgagcgc cgccgtgatc gtcgcgatcg aaccgatgag tgtaccgaag
      721 acgccggcta gaagcggcac ccaaaatcca gtaggcatct gcgctccccg gctcattctc
      781 aaggaataac agcctgcggt cctagcgttc cgccaagcgc cgcgcagacc gttgaggagc
      841 cgacatagac cttgccatcc gtgccgatga tcgactgacc gccacccacg cccgtctcga
      901 tctgctgtgc taccgcgccg acatcggcga cggcgcatgt gaacgccgtc acgccggttt
      961 tcagcgactc gatggcatgt tgtgttgccg gcgcatcgat ccacgaaaca gccttgttca
     1021 atgtctcgtc caagtcggcg cagcctgcca aatagaaagg gagcaccagc acgcccgcat
     1081 aaatcgcgta tttcattttc gtcctttcag gagggattta ttcgaaacga tcctggatcg
     1141 tctgttcgag atcaagcgtg agttgcggga tcttgccgtt tgagaactgt atgagcagtt
     1201 cgaagccgcc caatgccgca gcgagatcgt tcgcggcgat tgcgacgggc ggtacgaaga
     1261 cgccgaccac gcgcagaatg tcgtcgagcg tttcaatttc cccggaaaga ttggcggact
     1321 cgaaggcttg cgcggctttg ctgaagttga tttgtgaagc gtatttcagc acggtcgcaa
     1381 ccttctgcac atccgtcgcg agagacgaaa ggtcgctggt catgcgacag tcctttcggg
     1441 ctttatccgc gtggcgggcg cgagatcgag attggcaggg ctggcctgcg gtggcagtgc
     1501 tcccgatgcc gaaaggcgcg cgaccagcat ctggccgaga ttgtcgccaa gctctttgaa
     1561 aagacccggc gcctgagcga tgggatattg cgcggcgcga cgcagaactt cgttcgcgac
     1621 ggggatttcg agcaccttgc ctttttccgc gccctcaacg agtgcgaatc cagcgtcgat
     1681 cgcgcgcgtc atcacctgat cgatgtgcgc cgcgtacaac gcgcgcgcga tgtccggacc
     1741 gagcttcgcg gcgagccacg tgagcgcgag gcccgcgagc gaaatcgcga caggctggag
     1801 cgccttgaga acttcgttgc aaagcgcgcc ccatggcaga tcgacaagtg tgttacctgc
     1861 ggcatgggcc gacggcgcgg caagcgtcat cgccgccacg caaaaaatgc gcatgcgcat
     1921 gaagatatcc ctttggattt gccgtgaacc gcacggcgcg gatgacgcct cagcgtctaa
     1981 atgatgtttt aagcgtctaa acgttgtttt aaatgaattg cccgcgcttg ccggtcatgt
     2041 tgcgcctcaa aaggaggcca acatgaaggt caagacattc agcggcgcaa tgattctggc
     2101 gatgtcgatc ataggcgcgc cagccgcgca ggcgcatcat cggcatatgc gccatacgca
     2161 ccctcgcgaa ccagtgcaga cattttcaca cgagaccgag cgcccggaca cgacgcaacg
     2221 ggtggacgcc tatgtcaaca gcgaaggcga atatatccct tcgctgcttg aaccgagcgc
     2281 gccgtctgac caataagcat cagtggtcgg gcaccatgcc gttatattgg ccgccgagac
     2341 cgtcttcctt acgcaccgtg taataggcga cggacattgt cgtcatattg atgcaggtga
     2401 tgttgacgtt gacactttcc tgatcatccg tggcgttata gccgatcata gatttggcgc
     2461 tcatcgtctg cgtacaaacg cattgtcctg cgccatggca cgtgaagttc gcggcctcgg
     2521 cggagagcgg cgacaacccc agcgacagaa tggtgatcgc gattgccttt ttcattgttg
     2581 gctccattga tgaactggtc cggcatcctc acgaagcggc tgctttttcc attgcagcca
     2641 tttgcgcgag aacgcgtgcg acgacgtcgg ccttgaccac attcctcccc ggacaatcat
     2701 gatgatcgcg gcggcattct ttgtgaaaat gcaatccgcg tacgccaagc ctgaagccat
     2761 ccggccgcaa tccaagcttg cgatgtaaaa ctgcgagggc cgctaccgca ttatcgcgga
     2821 cttgcgcgcc cggcccgctc tcaaaacttt ccgccgcata atccccgacc atctccatcc
     2881 cgagcgtcag atgattccag cacgagacag agacaccgac ctgcgtcaga tcacagagat
     2941 tccaaatcaa atcctgtgcc acaaatagat ggaccccaga gtgccagtac aaaatgtcgc
     3001 gctcatagtg tttttgcgac actatccgtt gctgggcagg atgggccggg cccacccagg
     3061 tggcgaggct tggcacgccg gtattgtgca aggtgatgaa gcgcggtcgc tattcgcgcc
     3121 aggccagcgt gtcaacgaaa atggcgaatt ctgccggggc atatgccttg ggcgcggcga
     3181 gaaacgtcat cgcagcgccg ccagggccgc caccgcgcgg ccgtaagctg agcaatggga
     3241 gcgacacgcc caacgccgct attgtaggcg ctggcaacgg cgcaccagcc cgcctgccga
     3301 tgcatcgcca tcgcgagctt gagatagcgg acgcccactt cgataccggc ttggccgtag
     3361 agattgccga cgattccgac cttacgcgct gtccgcggca aaacctgcac ggtgccgcgc
     3421 tcccccgcgg caccgtgcat cgacatgcgc aagccggatt cgaaacgcac cacagcaagg
     3481 gcaaggcgag cgggaacgtc aagcgcttcg gccttcgctg caacgacggc ggcaacgtca
     3541 gcgcgcgcaa aaatcggacg cctcgtttgg ggcacgtcag cgatttgcgg ccgccaattt
     3601 gcgaaaggat cgagatcggc gcgatatgag gtgggtgcgg actatcgagc acttcaggcc
     3661 cgtgcagcct tgaaacccag aaactaattg ttgcatccgg taggggtaca atctcggcac
     3721 catgccgcac cgtgagtggg atgccaggcc atatccagac cgcgccaccg catgcgacga
     3781 gcgtgcgaca gcgttccggc gaacgcgaga gcgttgccgg cggattgagc ctgcataaag
     3841 aagtcctgtg agtaatgagt tagcttttta gggcttcaac gcgttgatgc ccggcacgag
     3901 gccgggcatg acgcggcagc gctctgccct acttcccgca atcggtcggc ggcgtttcgc
     3961 cggcaaatcg cgcgctcgtc caggcgacag cctcaagcgc gctgtgcatc gccgcgccgc
     4021 cgtgcccgac gcctggcatc aggaacagcc gcgccttgct cccgcccttg cagagcttgt
     4081 caaaataatc ctgcgtcacc gccggccgga tgaccgcatc gtcgagacct tgcgcgagga
     4141 acaccggaag atcgggcggc agcgggccgg gcgtgttctt cgccaggaga tcgcgccagg
     4201 gctgcacatc agccggatgc gcgacggtca gaaagtgctg ctccagcggc cgcgtcgtcc
     4261 ggttgcgggt cgaaatatcc cacggtccct cgatacattc ccgcgcgagc ttgtcgatcg
     4321 ccggcatggc gcgtggatcg acgacgcgat ccatcggcgc gccataaacg cgctgccagg
     4381 accagagagt catcgccgtg atgttcgtgc cggcgatcgt atcaagatcg tcgtccatca
     4441 gcgccgtgag ttcgcttgcc ggcgcagcgg cggcgacgcc gagcagcgtg agttccggcg
     4501 catagttctt ggcgatgagg ccggtgaaca gcgccgcgtg gccgccctgc gagtggcccc
     4561 agacaacaaa gcggctgcca ccgcctgcgc ccggcatact gcgcgcaaca cgcacgctgt
     4621 cgatgaccgc gcgcgcctcg ctcagcccga cgagataggg atgcggcccc agcgtgccga
     4681 ggccgggata atccgcc
//
LOCUS       DASWOG010000490         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_103783,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000490 DASWOG010000000
VERSION     DASWOG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103783"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGB93_10105"
     CDS             <1..624
                     /locus_tag="VGB93_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146480.1"
                     /translation="SLATQGYVVSEAKDGRSALDAIRAKAADVVVLDLGLPDMDGIEI
                     IRQLRGAGETLPIIVLSSRETEGAKVAALDLGADDYVTKPFGIDELLARIRAAQRHRL
                     QQQGEKAIFRNGDLTADLVRRIITVRGQEVKFSPREYDVLRLLIAHAGKVLTHRFILR
                     EVWGGDADVQYLRIYIRALRQKIERDPERPRHILTETGVGYRLALQE"
     gene            complement(654..1388)
                     /locus_tag="VGB93_10110"
     CDS             complement(654..1388)
                     /locus_tag="VGB93_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0146481.1"
                     /translation="MLYKDIRERGRPCVACPAGYPPACSVLIDPQAEEFGRFQQRLAQ
                     NFEIFEPHRNVYLRNAKSDDIYILCDGWAFTFLQTADGRRQIISFLVCGDFFSPSILF
                     EETSALSLRTLTKALFCRMKRADVRKKVVSSDETLDVIGKIGAANERAKNELLLDLGR
                     RNADERIAHLMLTLTERLDRLGTVQGGAYPFPLRQRDMADATGLTPVHVGRTIAKFRE
                     SGLIELSAGFLRILNRAELHRIGCID"
     gene            1644..>2956
                     /locus_tag="VGB93_10115"
     CDS             1644..>2956
                     /locus_tag="VGB93_10115"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEY0146482.1"
                     /translation="MNRRPRGRSSLVSKSARVTRNDTVDSNIPRRLDCPFWSQAMSVD
                     VLADQLKFMNLGADAQKNIQSVKTIITQELPGALDTFYAQVRRFPETVKFFSNEAQIV
                     SAKSRQLAHWDAISSGRFDGDYVRAASAVGDIHAKIGLEPRWYIGGYALVVEVLVAKV
                     MEARFPKHRFGSRRMPVQQVAAELGALIKATMLDMDFAISAYLRAAETARKRAEEAVL
                     AQEREDVSASVGKAMSSLSDGDLTCRLADDIPVEYQQLRIDFNSSMEKLQQTVLAVSA
                     NAQAIRSGTEQIAVAADDLSHRTERQAASLEETAAALDEITATVKKTAENANYAHVTV
                     SAAKMEAEKAGVVVSEAMHAMSGIEKSSHQISQIISVIDEIAFQTNLLALNAGVEAAR
                     AGDAGRGFAVVAMEVRGLAQRSAEAAKEIKGLISASTTQVAQGVH"
BASE COUNT          678 a          859 c          830 g          589 t
ORIGIN      
        1 agtctcgcga cacagggcta tgtcgtctct gaagcgaagg acgggcgcag cgccctcgac
       61 gcgatccgcg cgaaggcagc cgatgtcgtc gtcctcgatc tcggcctgcc cgacatggat
      121 ggaatcgaga tcatccgcca attgcgcggc gccggcgaaa cgttgccaat catcgttttg
      181 tcgagccgcg agacagaggg cgcgaaagtc gcggcgcttg atctcggtgc agatgactac
      241 gtcaccaagc ctttcggcat cgacgaactg ctggcacgca tccgcgcggc gcaacgtcac
      301 cgcctccagc agcaaggtga aaaagcgatt ttccgcaacg gcgatttgac cgccgatctc
      361 gtccgccgga tcatcaccgt ccgcggccag gaggtgaaat tttcaccgag agagtatgat
      421 gttctacggt tgctgatcgc gcatgccggc aaggtcctga cgcatcgttt tatcctgcgt
      481 gaggtctggg gcggcgatgc cgatgtccaa tatctgcgca tttacatccg cgcgctgcgg
      541 cagaaaatag agcgtgaccc cgagcggcca cgccacatcc tcacggaaac aggcgttggc
      601 tatcggctcg cacttcagga atagacgccg cgcagaacct gtcgccgcca atttcagtcg
      661 atgcagccga tgcggtggag ttcggcacgg ttgaggattc tcaagaatcc cgcggacaat
      721 tcgatcagcc cggactcgcg aaatttggcg atcgtccggc cgacatgaac tggcgtgagc
      781 cccgtggcat ctgccatgtc gcgctggcgt agcggaaaag gataggcgcc tccttgaacc
      841 gtgcccagac gatccagacg ttccgtcagg gtcagcatca aatgcgcgat ccgctcatcg
      901 gcgttgcggc ggccaagatc gagcaggagt tcattcttcg cccgctcgtt cgcggcgcca
      961 atcttcccga tgacgtccaa tgtctcgtcg gacgaaacga ccttctttct cacgtcggca
     1021 cgtttcatgc ggcagaacag tgcctttgtc agcgttcgca gcgacagggc cgatgtctcc
     1081 tcgaacaata tgctgggcga gaagaaatcc ccacacacca ggaacgagat gatctggcgc
     1141 ctgccatccg ccgtttgtag aaacgtgaac gcccagccgt cgcacaaaat atagatatcg
     1201 tctgacttgg cattgcgcag atagacattg cggtgaggtt caaagatctc gaagttttgc
     1261 gcaaggcgtt gctgaaatcg gccaaactct tcggcttgag gatcaatcag tacactgcag
     1321 gctggcggat atccggccgg gcatgcaacg caaggccggc cgcgttcgcg aatgtcctta
     1381 taaagcattc cgactctctg gcggcggaac ctacgccgct acgataagca catagataat
     1441 atgaattggc aacgctggtc ctatttggcg acatacccaa ccgcgcacaa gatatgttca
     1501 caatctatgt tgtaaatcta agtataataa tatcaattat tgaaaatatt tttcgatgtc
     1561 ttgttgaaaa acggcataag tgtgaacttt acatggacca ttcagtcgca ccaacttacc
     1621 aaaagtagat cttattttat ttggtgaatc gcaggccacg cggccgatct tcgttagtca
     1681 gtaaaagcgc acgcgttacg cgaaacgaca cagtagattc taatattccg cggcggttgg
     1741 attgcccttt ttggagccaa gcgatgtcag tggacgtgct agccgaccaa ctcaaattca
     1801 tgaaccttgg ggccgacgct cagaagaata ttcagtcggt caaaaccatc atcacccagg
     1861 aattgcctgg cgcgctcgat acgttttatg cgcaagtgcg ccgcttcccc gaaacggtca
     1921 aattcttctc aaacgaagcc cagattgtga gcgccaaaag cagacagctt gctcactggg
     1981 acgcgatatc aagcggccgg tttgacggcg actatgtcag agccgcctcg gccgtcggcg
     2041 atattcacgc aaagatcggc cttgagccgc gctggtacat cggcggctat gctctcgtgg
     2101 tcgaggtgct cgttgcgaaa gttatggagg cgcggttccc caagcaccgt ttcggatcgc
     2161 gacgcatgcc cgtccaacag gtcgcagcag aactcggcgc actcatcaag gcaactatgc
     2221 tcgacatgga ctttgcgatt tcggcttatc tcagagcggc cgaaaccgcg cgtaaaaggg
     2281 cggaagaagc cgtgctcgcg caagaacgcg aggatgtgtc ggcttccgtc ggcaaggcca
     2341 tgtcctctct ctcggacggc gatcttacct gccgattggc ggacgacatt cctgtcgaat
     2401 accagcaact gcgcatcgac ttcaacagct caatggagaa gctgcagcaa accgtgttgg
     2461 cggtcagcgc caacgcgcag gccattcgct cgggcaccga gcagattgcc gtcgccgccg
     2521 acgacctgtc acaccgcacc gagagacaag cggccagcct ggaagagacg gccgccgccc
     2581 tcgacgaaat taccgcgacg gtcaaaaaga ctgctgagaa cgcaaactat gcccatgtca
     2641 ccgtctccgc ggcaaagatg gaggcggaaa aagccggcgt cgtggtgagc gaagcgatgc
     2701 acgctatgtc cggcatcgag aaatcatccc accagattag tcagatcatc agcgtcatcg
     2761 acgagatcgc cttccaaacg aaccttctcg ccttgaacgc cggcgtagaa gccgcccgcg
     2821 cgggcgacgc tggccgcggt tttgccgtcg tcgcgatgga agtgcgcggg ttagcgcaac
     2881 ggtcggcaga ggccgccaag gaaatcaaag gattgatttc ggcctcaacc acccaggtcg
     2941 ctcagggcgt gcattt
//
LOCUS       DASWOG010000491         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_47957,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000491 DASWOG010000000
VERSION     DASWOG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47957"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGB93_10120"
     CDS             <1..306
                     /locus_tag="VGB93_10120"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY0146483.1"
                     /translation="ALADSFGSVEEAKKQLVQGGVGQFGSGWTWFALKDGKIEVMKTA
                     NGENPLIFGAIPILGIDVWEHSYYIDYRNRRLDYLQAVVDHLVNWEYVAERFETGEA"
     gene            461..1996
                     /gene="murJ"
                     /locus_tag="VGB93_10125"
     CDS             461..1996
                     /gene="murJ"
                     /locus_tag="VGB93_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0146484.1"
                     /translation="MYRNLFSVAVLTLVSRATGFIRDVILAAVLGAGLNADAFVVAFR
                     LPNHFRAIFGEGAFNAAYVPCYSQILAKRGAEAGRHFASQIFTLLLASQVVLLAAALI
                     FMPQFIDLLAPGFRADPQKFALAVTLTRITFPYLLFITLVTLQSGTLNALEHFTAAAS
                     APILLNVVMIFFLALAFLFPSAGIAAAVGVTVSGVAQFALTFMALRRIGLHEHLVRDI
                     WNADVKHFFVILGPAVIGSAGVQLALFADTIIGSMLPTGGVSSIYYADRIYQLPIGVV
                     GIAAGTVLLPEMSRRLVRGDADGAYHAQNRTMALSVTIAAPCFVAFIMIPEIIMRGVF
                     MHGAFNEGDALAAAAVLTAYGFGIVPIVLIRSAVASFQSIADTRTPMYISLFAVAVNV
                     GLKLLLFHPFGAAGLAAATATGAWINLLLLMAIASRQGLMRLDWVFGKSAAATSIAGF
                     ALALFALLAAWPVAEFCARLPRFQNESELILLLIGGGVVYALVLGAGLYATGVRLRRS
                     ASV"
     gene            complement(2351..3425)
                     /locus_tag="VGB93_10130"
                     /pseudo
     CDS             complement(2351..3425)
                     /locus_tag="VGB93_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860716.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
BASE COUNT          592 a          996 c         1023 g          814 t
ORIGIN      
        1 gcgctcgccg attcattcgg ctccgttgaa gaggcgaaga agcagctcgt tcagggcggc
       61 gtcggccaat tcggctccgg ctggacgtgg ttcgcgctta aggacggcaa aatcgaagtc
      121 atgaagaccg ccaatggcga aaacccgctg atcttcggtg caattccaat tcttggcatc
      181 gatgtctggg aacattccta ttacatcgac taccgcaatc gtcgtctcga ttatctgcaa
      241 gctgtcgtcg atcacctcgt caattgggaa tatgttgccg agcgcttcga aaccggcgaa
      301 gcttaaacta ggtttcgcct gaattgcggc gataaaaggt cggccaagca tttggccgac
      361 ctttcatttt gtgtaccttg gtcgcaacgg cgtgcgtttt gacttggcgc cgaaataaaa
      421 agatcgtggt cctccgcgat tcgcgggggc ggacgcttgg atgtatagaa accttttctc
      481 cgtcgcagtg ctgacgctcg tttcgcgcgc gacgggattt atccgcgacg tcattctggc
      541 cgccgtgctt ggcgcgggcc tcaatgccga tgcgtttgtc gtggctttcc ggctgccaaa
      601 ccattttcgt gcgatcttcg gcgaaggtgc gttcaacgcc gcctatgtgc cttgctattc
      661 gcagattctg gcgaaacgcg gcgccgaggc ggggcgccat ttcgccagcc agatattcac
      721 actgttgttg gcctcgcagg tcgtgctgct ggctgcggcg cttattttca tgccgcagtt
      781 tattgatctt ctggcgccgg gatttcgtgc tgatccgcag aaattcgcgc tcgccgtgac
      841 gctgactcgg atcacttttc cctatttgct tttcatcacg ctcgtcacat tgcaatcagg
      901 cacgttgaac gcgctggagc atttcaccgc cgcggcctcc gcgccgattt tgctgaacgt
      961 cgtgatgatc ttctttctgg cattggcgtt tcttttccca agcgctggaa tcgctgcggc
     1021 ggtcggcgtc accgtttcag gcgttgcgca attcgctctg acgttcatgg cgctgcggcg
     1081 gatcggcctg cacgaacatc ttgtgcgtga catctggaat gccgacgtca agcatttctt
     1141 cgtcatcctc ggccccgcgg taatcggctc ggccggtgta caacttgcac ttttcgccga
     1201 tacgatcatc ggctcgatgc tgccgacagg cggcgtttct tcaatctatt atgccgatcg
     1261 tatctatcaa ttgccgatcg gcgtcgtcgg cattgccgcc ggaaccgtgt tgctgccaga
     1321 gatgagccgc cgtctggtgc gtggcgacgc agacggcgct tatcacgcgc agaaccggac
     1381 gatggccctg tcggtgacaa ttgccgcgcc gtgtttcgtc gccttcatca tgatcccgga
     1441 gatcatcatg cggggcgttt tcatgcatgg cgcctttaac gaaggtgatg cacttgcagc
     1501 tgccgccgtg ctgacggcct atggcttcgg gatcgtcccg atcgtgttga tccgttcggc
     1561 ggtcgcgagt tttcaatcga tcgccgacac gcgcacgcca atgtacattt cgcttttcgc
     1621 cgtggccgtg aacgtcgggc tgaaactgct gttgttccac ccgttcggcg cggccggtct
     1681 cgctgcggcg accgcgacgg gcgcctggat caatctgctt ctgctgatgg cgattgcctc
     1741 gcggcagggc ctgatgcggc tcgattgggt gttcggcaag agcgcagcgg cgacatcgat
     1801 cgcaggcttt gcgttggcgc tcttcgcttt gctcgcggca tggcctgtgg cggaattttg
     1861 cgcccgcttg ccgcgtttcc agaacgagag cgagttgatc ctgctgctca tcggcggtgg
     1921 cgtggtctat gcgcttgtgc tgggagcagg gctttatgca acgggtgtga ggttgcggcg
     1981 ctctgcaagc gtgtgaagtc tcgcttccgc acagcagact tcttttccgt aacggaggcg
     2041 cgcgcctcgc catattccaa gctggaactg caccatgaca cgcgtcaaaa ttgcgacaaa
     2101 aattactttg gaaatttccc tagtttaaac tgccgcagcg atttttatga accaaggagg
     2161 gcggcccttc gtttgtgcgg ggaggtcgcc cttctcgatc ctcgaaaagg attcaatgag
     2221 atacgcatcg agtcgttaaa ttcggacgaa gtcgatgaag gaccgttcag cctctatgaa
     2281 ctgggcgatc caattgttta gggcaggcgg ccgcacgacg cgtatcgaga agtgacgtac
     2341 aagttgcgcc ttagaacagc ccttcgatct ggcccttgtc gtcgagcttg atgttcaccg
     2401 ccgccggcac cttcggcaga ccgggcatgg tcatgatctc accggtgatc atcacgacaa
     2461 agccggcgcc ggccgagagc cgcacttcac gcacgccgac gatgtggccg gtcggcgcgc
     2521 cgagcttggt cggatcggtc gagaaggaat attgcgtctt cgccacgcag acgggcaaat
     2581 ggccgaagcc aagcgcctcg atatcggcga tctgcttctt ggccgcgggc tcgatcgaaa
     2641 tgtccttcgc gccgtagatc tcttgcgcga ttttgcgcat tttctcggtg agcgaaagct
     2701 cgtcggggta gagcacttgg aaattggctt tgccagattc ggcgagttcg acaaccttct
     2761 cggcgagctt gaccgcgcct ttgccgccct cagcccaatg tttgcaaacg atcgcctccg
     2821 cgcctaattc gtcgcgcgag gtcttggcga tgatgtcgag ttagtcctcg tgatcgccgc
     2881 cgaaatggtt gatcgcgacg accaccggta cgccgaactt gcgcacattg gcgacatggc
     2941 ggccgagatt ggcgaggccg gccttcaccg cagcgtggtt ttccttgccg agatcggctt
     3001 tgacgacgcc gccctgtgcc ttgagcgcac gcagcgtcgc gacgatgacg gcggcggatg
     3061 gtgcgaggcc cgccttgcgg cacttgatgt ccatgaattt ttccgcgccg aggtcggcgc
     3121 cgaagccggc ttccgtcacc gtgtaatcgg ccagcgcgag gcccgcggtc gtcgcgatca
     3181 ccgaattgca gccatgcgcg atattggcga aaggaccgcc gtgaatgaag gccggcgtgc
     3241 cctccagcgt ttgcaccaag ttcggcatca gagcgtcctt gagtagcacg gtcatcgcac
     3301 cggcagcctt caggtcttcg gctgttatcg gctttttgtc tgggccataa gcaacaacga
     3361 ttttgcccag ccgatcttgc agatcggcga gattgcgggc gaggcagaag atcgccatca
     3421 cttcc
//
LOCUS       DASWOG010000492         7410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_47975,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000492 DASWOG010000000
VERSION     DASWOG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47975"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..663)
                     /locus_tag="VGB93_10135"
     CDS             complement(160..663)
                     /locus_tag="VGB93_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY0146485.1"
                     /translation="MSWTDERIELLRRLWLEGLSASQIARELANGISRNAVIGKVHRL
                     GMSGRVKAAAPASVRPKVKTARPVIHQRMSAPMMHGNLALKSQPMEAPLPAPREDVVI
                     PMSEMVTIMELKESMCRWPMGDPSSGEFRFCGAKKGANQSGPYCSYHARIAYQPVQDR
                     RRRLQSA"
     gene            complement(1045..1383)
                     /locus_tag="VGB93_10140"
     CDS             complement(1045..1383)
                     /locus_tag="VGB93_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0146486.1"
                     /translation="MSSDESLSCGHTGGYRLTPRALDDLDDIWRFSAETWSIKQADRY
                     IDELARVFETIAIMPTLARERNEFAPPVRIHPHESHLIVYTIEADHITILRSLGGQQE
                     WISLLKAADL"
     gene            complement(1370..1615)
                     /locus_tag="VGB93_10145"
     CDS             complement(1370..1615)
                     /locus_tag="VGB93_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY0146487.1"
                     /translation="MATMNVSLPEPMKAWVERQTEGGLYGNASDYIRDLIRKDQQRKE
                     AIAALQAAITQGLESGEPQPFDPEAFKLRMRERHVVR"
     gene            1934..3139
                     /locus_tag="VGB93_10150"
     CDS             1934..3139
                     /locus_tag="VGB93_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0146488.1"
                     /translation="MTSSLLPTYARFNLAFERGEGAWLYATNGEKYLDFGAGIAVVSV
                     GHSHPHLVEALTEQGRKLWHVSNLFEIPQAERLGARLVAESFADLVFFTNSGTEAMEG
                     AIKTARRHQFVSGHPERYRIISFLGAFHGRTLAAVAAGGNPKYLEGFGPPHEGFDPVP
                     FGDLAAVKAAITPQTAAILIEPIQGEGGVRVATPEFLEALRALCDARGLLLILDEVQS
                     GMGRTGKLFAYEHSRAAPDIMALAKGVGGGFPLGAFLTTRKVGECMTPGTHGTTYGGN
                     PLATAVGNAVLDLILADGFLDHVTKMAAALDHGLRELQVRYPDVIEEVRGKGLLSGIK
                     VAGPVVEFVNAALAEKLITIPASDNVVRLLPPLTVNEVEIALALTRLNSAAETLSVAR
                     AKAARRGAA"
     gene            3136..4089
                     /gene="argF"
                     /locus_tag="VGB93_10155"
     CDS             3136..4089
                     /gene="argF"
                     /locus_tag="VGB93_10155"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0146489.1"
                     /translation="MTSSALSLETRPKHFLDLSEVSAEELRRILSLAGKIKGRRRKGQ
                     LADVRPLTGKVLAMIFDKPSTRTRVSFDVAMRELGGETIMLTGKEMQLGRGETIADTA
                     RVLSRFVDAIVIRVLDHGELAELARNAEVPVVNGLTKKSHPCQVMADVLTFEEHRGPI
                     KDRTVAWTGDANNVLTSWIHAAQRFDFTINVASPAELDLPLEVQSWAKANNARLNITR
                     DPFEAVRGADAVISDCWVSMGDKDESFRHNLLAPYQVNSKLMQAAAKDAVFMHCLPAH
                     RGEEVTDEVIDGPQSVVFDEAENRLHAQKGILAWCFDAVAG"
     gene            4086..5057
                     /locus_tag="VGB93_10160"
     CDS             4086..5057
                     /locus_tag="VGB93_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEY0146490.1"
                     /translation="MSEAETPLRPVSDQAHDDMVLPFAVEPLDVRGRVVRLGPAIDEI
                     LTRHAYPPPVARLVGEAAALTVLLGAALKSEGRLQLQTRSDGIVNMIVVDFDAPDRLR
                     AFARFDEAKLAELAPGDELLGHGHLALTIEQGSDVARYQGVVALEGQGLGEAAHQYFR
                     QSEQIPTFVHLAVAESLTGAGTNWRAGGLLLQFLPASVERQRQADLAPGDAPQGAILP
                     ETLEDDAWAEAKALAATLEDHELVDPGLSSERLLFRLFHERGVKVFAPQVVRAACRCS
                     DERIAAMLRNFTQAERDDMIGENGKIGVTCEFCSTHREFEPKDFAVE"
     gene            complement(5683..5919)
                     /locus_tag="VGB93_10165"
     CDS             complement(5683..5919)
                     /locus_tag="VGB93_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0146491.1"
                     /translation="MTTTVTSKGQVTIPKEVRELLEIGPGSLIDFRRDSSGAITLVKV
                     EKTARPNRFARLRGHAGKGLSTDEIMAMTRDELK"
     gene            complement(6358..6861)
                     /locus_tag="VGB93_10170"
     CDS             complement(6358..6861)
                     /locus_tag="VGB93_10170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0146492.1"
                     /translation="MARRIEKLQREHRVEEFVCGKEPLDRFLIRFALQSQLANSAQTY
                     VALSGSEIIGYHTLTYGDVAYEDSPERLRKGVARHPVPLMILARLAVSRTWSGKGIAA
                     GLLKDALTRTLAAADIAGLRAFAVHAKDGEARTFYEHFGFVASPSDPMHLFILLKDVR
                     ALIDPKA"
     gene            complement(6867..7154)
                     /locus_tag="VGB93_10175"
     CDS             complement(6867..7154)
                     /locus_tag="VGB93_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY0146493.1"
                     /translation="MANAIRSEKLDLRLTPEAKRVLNAAARASRRSVSEFVLESALAR
                     AEEALPDRQHFGLDAKHWQAFLAALDAPPRPLPRLKRLLSETSVFEQQPVD"
BASE COUNT         1395 a         2078 c         2325 g         1612 t
ORIGIN      
        1 tggtcggtgc tcgccaaatt cgatggcgaa ttcgctggta attcgcaaac ctatggcgga
       61 accggaacac tgcaatatac gtggtaaagc tccacggatt gcttcgcggg agtttgccgc
      121 cggccggacg tagtccgacc ggttggcttg cgacgctgat taggccgatt gcaggcgtcg
      181 gcgacgatcc tgcaccggct ggtaggcaat acgggcgtga tagctgcaat aagggccgct
      241 ctgattggcg ccttttttgg cgccgcagaa gcggaattcg ccggaggatg gatcgcccat
      301 cggccaacgg cacatggatt ccttcagctc catgatcgtg accatttcgg acatcgggat
      361 gacgacatcc tcccgaggtg cgggaagcgg cgcctccata ggctgtgatt taagggcgag
      421 attgccgtgc atcattggcg cgctcatgcg ctgatggata accggccgcg ccgttttcac
      481 cttgggccgg acggaggctg gagccgcggc cttgacccgc ccggacatgc cgaggcggtg
      541 gactttgcca atgacggcgt tgcggctgat gccgtttgca agttcgcgcg cgatctggct
      601 ggcgctgagc ccttcgagcc agagtctgcg cagcagttca attcgttcat cggtccagga
      661 catttagtcc tcctcgattt tgatcggaaa gtttggctga acgcgattga cgcaatccgg
      721 cgctcctgaa gcgacgggcg gaatccggaa tcgacgcagc gccggaaaac cggcgtcgta
      781 caagaagtta tgcatttgaa aggcccgccg cacgagatgt cgtagtcgac ttcagagaag
      841 ctacactagc tgttgaatct agcgcaagag tcgccatgtc ttgcagcaca acgttttcaa
      901 caagcgtcct agtcgaacgg gcgcccgcgt cattgtgact cgggcccatg ccgcaaaacg
      961 cccgtggccc ggggtggctc aatttgaaat tcgccgcccg gccgcattct cttctttagg
     1021 tgttcgtggc gtgatagtga ggcttcacaa gtccgccgct tttaggagtg aaatccattc
     1081 ctgctgcccg cctaacgaac gcaagatagt gatgtgatcg gcttcaatcg tgtaaacgat
     1141 tagatggctt tcgtgcggat ggatacggac aggcggggca aattcattac gctctcgcgc
     1201 taaggtcggc atgatcgcga ttgtctcgaa gacgcgcgcc agctcgtcga tatagcgatc
     1261 cgcctgtttg atcgaccaag tttcggctga aaatcgccag atgtcatcga gatcgtcgag
     1321 cgcacgcggc gtaagccgat agcctccggt atgtccgcac gacagcgatt catcggacga
     1381 catgacgctc ccgcattcgg agcttgaagg cttccgggtc gaagggctgt ggctcgccgc
     1441 tttcaagccc ttgggtgatc gctgcctgca aagcagcaat ggcctctttg cgctgctgat
     1501 ctttgcggat caggtcgcgg atataatcgc ttgcattgcc gtagaggccg ccttccgttt
     1561 gacgctcgac ccaggctttc attggctctg gtagtgaaac attcatcgtg gccacgggag
     1621 tctcctttta aggaaatctt accaatgact accaataatt gtcaaattat atggcaatga
     1681 ttgccatcat gtagagggtt tgcgggcttg gccatggggt tgtgcacaag ggaggcttcg
     1741 cgatcagctg gctgcccgaa atttcaaatt gaggcccaac cggctggggc aatgatttga
     1801 ccgctgtgcg caggccccgt aaaatatgag ttcgtgttgc cgctcccccg gggcggcact
     1861 tttctttggg agaccgccca atccggcgtc acgggcaaga ccgcgcaccc ggatttttac
     1921 tggagctttt gctgtgacct cgtcgcttct ccccacctac gcgcgcttca atcttgcttt
     1981 cgagcgggga gaaggagcct ggctctatgc gacgaacggc gagaaatacc tcgatttcgg
     2041 tgccggcatt gccgtcgtca gtgtcggtca ttcgcatcca catctcgtcg aagctttgac
     2101 cgagcagggc aggaagctct ggcacgtgtc caatcttttc gagattccgc aggcggagcg
     2161 gcttggcgcg cggcttgtcg cggaaagctt tgccgatctg gtgttcttta ccaattccgg
     2221 caccgaagcg atggaaggcg cgatcaagac cgcgcgccgc catcaattcg tcagcggcca
     2281 tccagagcgc tatcggatca tcagcttcct gggcgccttt cacggccgca cacttgcagc
     2341 ggtggccgct ggcggcaatc cgaaatatct cgaaggcttc ggaccgccgc acgaggggtt
     2401 cgatccggtt cccttcggcg atctcgcagc ggtgaaagcg gcgatcacgc cgcaaacagc
     2461 ggcgatcctg atcgagccga tccagggcga gggcggcgtc cgcgtcgcga cgccggaatt
     2521 cctggaagcc ttgcgcgcac tctgcgatgc gcgcgggctc ttactcatcc tggacgaagt
     2581 gcaaagcggc atgggccgca ccggtaaact ctttgcctat gagcattcgc gcgccgcgcc
     2641 ggacatcatg gctcttgcca agggcgttgg cggcggtttt ccactcggcg cgttcctcac
     2701 cacgcgcaag gtgggcgaat gcatgacgcc tggcacgcac ggcacgacct atggcggcaa
     2761 tcccctggca acggccgtag gcaatgcggt gctcgacttg atcctggccg acggttttct
     2821 cgaccacgtt acgaagatgg ccgcggcgct cgatcatggc ctgcgcgaat tgcaggtccg
     2881 ctatcccgac gtgatcgagg aggtgcgcgg caaggggctg ctgtccggca tcaaggttgc
     2941 cgggccggtt gtcgaattcg tcaatgcggc gctggcggaa aagctcatca ccatcccggc
     3001 gagcgataat gtcgtccgtc tgttgccgcc gctgacggtc aacgaagtcg aaatcgcatt
     3061 ggcgctgacc cggctcaatt ccgccgcgga gactctttct gtggcgcggg cgaaggccgc
     3121 gcggcggggg gccgcatgac cagctcggct ttatcgctgg aaacccgacc gaagcatttc
     3181 ctcgacctct cggaagtgtc ggccgaggaa ttgcggcgca tcctgtcgct cgccggcaag
     3241 atcaagggcc gacggcgtaa gggtcagctc gccgacgttc gaccactgac cggcaaagtg
     3301 ctggcgatga ttttcgacaa gccctcgacc cgcacgcgcg tctccttcga tgtggcgatg
     3361 cgtgagctcg gcggcgaaac gatcatgctg accgggaagg aaatgcagct tggccgcggc
     3421 gagacgattg ccgacacggc gcgtgtgctt tcgcgattcg tcgatgccat cgtcatccgt
     3481 gtgctcgacc atggtgaact cgccgaactg gcgcgtaacg ccgaagttcc ggtcgtcaac
     3541 ggtctgacca aaaaatcgca cccgtgccag gtgatggcgg acgtcctcac gttcgaggag
     3601 catcgtgggc cgatcaaaga tcgtacggtc gcgtggacgg gcgacgcgaa taatgtactc
     3661 acgagttgga ttcacgccgc gcaacggttt gatttcacca tcaatgtcgc atcgccggcg
     3721 gagctcgatc tgccgctcga ggtccaatcc tgggcgaagg cgaataacgc acggctcaat
     3781 atcacgcgtg atccttttga agcggtgcgc ggcgccgatg ccgtgatctc ggattgctgg
     3841 gtgtcgatgg gcgacaagga cgagagtttc cgtcacaact tgctcgcgcc ctatcaggtc
     3901 aattccaaac tgatgcaggc ggcggccaag gacgcggtct tcatgcattg ccttcccgcg
     3961 catcgcggcg aagaggtgac ggacgaggtg atcgatgggc cgcaatcggt ggtcttcgac
     4021 gaggccgaga accggctgca tgcgcagaaa ggcattctcg cttggtgttt cgacgcggtg
     4081 gcgggatgag cgaggcggaa acgccgctgc ggccggtctc cgatcaggct cacgacgata
     4141 tggtgctgcc gttcgcggtc gagccgctcg acgtgcgcgg ccgcgtcgtg cgtttggggc
     4201 cggcgatcga cgaaattctc acccgtcatg cctatccgcc gccggtcgcg cggcttgtcg
     4261 gcgaggcggc ggcgctcacg gttctgctgg gcgcggcgct aaagtcagaa ggccggctgc
     4321 aattgcagac ccgcagtgat ggcatcgtca atatgatcgt cgtcgatttc gacgctccgg
     4381 accggctgcg cgcttttgcg cggttcgacg aggccaagct cgcagagttg gcgccaggcg
     4441 acgaattgct cggccacgga catctggcgc tgacgatcga gcagggcagc gatgtcgccc
     4501 gctatcaggg cgtcgtcgct cttgaaggtc aggggctggg cgaggcggcg catcaatatt
     4561 tccgtcagtc agaacagatc ccaaccttcg tgcatcttgc cgtggccgag agtttgaccg
     4621 gtgcgggaac gaattggcgg gcgggaggcc tgctgctgca gtttttgccc gcttcggtcg
     4681 agcgccagcg ccaggcggac cttgcgcccg gcgatgcccc ccaaggcgct attttgcctg
     4741 agacgcttga ggacgatgcc tgggccgagg cgaaagcact cgctgcaacg ctggaagatc
     4801 acgaattggt cgatcctggc ctctcgagcg aacggctgtt gttccggctg tttcacgagc
     4861 gtggggtcaa ggtcttcgca ccgcaggtcg tccgcgcagc atgccgctgc tcggacgagc
     4921 gcattgccgc gatgctgcgg aatttcacgc aagccgaacg cgacgacatg atcggtgaaa
     4981 acggcaagat tggcgtgacc tgcgaattct gctcgaccca tcgcgaattc gagccaaaag
     5041 attttgccgt ggaatagtcg agtgtgatcg aggccgctcc ggtcatattg tcgacgcgct
     5101 ccacccacct ccctagcgat gtggtcgttg caatttctca atgtctttgc ggggcaaggc
     5161 ctttttccca gtcgtcgccg ctgtggccag aagagcccag ctattgaaca ctcgctcgcg
     5221 cctgtcggcc ctcgtagaat gagcgggagg cgtggcaccc gtcggcgttt gtgattattc
     5281 cggggcaatt agctccagcc cgggaaaata ggtgcgacag cggcttggat cacgggtgat
     5341 aagcggcgag ccggcgacgg cagtatgtgc gccgataaag aagtctggca acacgctggt
     5401 gcgcgttccg ccattggagc gatactgcaa gaagaccttg ccggggagaa acaaggccgt
     5461 gcgtgggatg ggaaccgttt cgatctgtgc tttcggtctg tgcggtaatt agcatggcgt
     5521 cgaggtcttc gatccgttta tatcggatcg atgtttctgc ataggacgtc atttataacg
     5581 attggtccgg caagcgccgc ctcttcgagg gggctataga ccattccgcc cagttcggat
     5641 cgtcagtgac aaggtcaagc agcacgtttg tatcgacgaa gatcacttta gttcatcgcg
     5701 agtcattgcc atgatttcgt cggtgcttag gccttttccg gcgtgcccgc gcaggcgcgc
     5761 gaaacggttc gggcgggccg tcttttccac tttgacaagg gtgattgcgc cactgctgtc
     5821 gcgtctgaaa tcgattaggc tgcccgggcc gatttcgagc agctctctaa cctcttttgg
     5881 aatagtaacc tggcccttgc tggtgacagt cgtggtcatg gcgagtccgg taaggcgttg
     5941 ctctaatttg gtattactta tctcatatcg cacgcttttt cgagctttgg tgttagttcc
     6001 aaagagtgcg gtttcggcat tgaagctggg tgagtgagtt cgcaattttc taagtttcgc
     6061 agcggcttag ccttctttta tcttagcaga gccgtcgtgc agggtatttc gcgctctttg
     6121 ttgcttttgc ctttttcaag aaacgcaaca agcggaggct ttgaagcgcg atcccggggg
     6181 ccggtgttgc tatttgtaga gctcagcagt cgacgggcga tttgtccttg agcgcggtgg
     6241 ggtgggtgag ttgttgcctc ttcaggggtc ggtgcatcct cgtgcaaatg caagactaac
     6301 gccgctgtgg tgaaaaatct tgagactccg ccattgggtg gtgccttcag ttttgattta
     6361 ggctttgggg tcaatcaggg cgcggacgtc cttgaggagg atgaataagt gcatcggatc
     6421 gctgggcgag gcaacgaaac cgaaatgctc gtagaaggtt cgcgcctccc cgtctttggc
     6481 gtggacggca aaggcgcgca ggcccgcaat atcggcggcg gcgagcgtcc gagtcagcgc
     6541 gtctttcagc aaaccggcag cgattccttt gcccgaccaa gtacgagaaa ccgcgaggcg
     6601 cgcaaggatc atcaaaggca caggatgtcg ggctacgccc ttgcgtagcc gctcgggact
     6661 gtcctcatag gctacgtcgc cataggttag tgtgtgatag ccaattattt cgctgcctga
     6721 gagtgcgaca taggtctgcg cgctgttggc aagctggctt tgcaaggcaa agcggatcag
     6781 gaagcggtcg agcggttcct taccacaaac gaattcctcg acgcgatgtt cgcgctgcag
     6841 cttctcgatg cggcgtgcca tggcaattaa tcgacaggtt gctgctcgaa cacgctagtc
     6901 tcactcagaa gccgcttgag ccgcggcaaa ggacgcggcg gcgcatcgag tgcggcaagg
     6961 aatgcctgcc aatgtttggc atcaagcccg aaatgctggc gatctggcaa cgcttcctcg
     7021 gcacgggcaa gcgcgctctc cagcacaaac tcgctgactg accggcgcga ggcacgggca
     7081 gctgcgttta gcacgcgctt agcttctggc gttagccgca ggtcgagttt ctcagatcgg
     7141 attgcattgg ccatgatggc gcctcttgcc ttacatatag cacgccgagc caagtttgtc
     7201 atgactttgt ctggcataag gttagcttgg aaggggagtt ttttagtttc gcgtccagag
     7261 tcacggttgc agtgccgttt ggaggctaaa agcaacatcc acgaaaccgt tcgggtgttc
     7321 caaagagtgc gatttcggtc gcgttgctga tgagcgagtg atcgtgactc tctgatttga
     7381 aggccacagg cggggcgaat gcctgacatc
//
LOCUS       DASWOG010000493         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_323885,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000493 DASWOG010000000
VERSION     DASWOG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323885"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGB93_10180"
     CDS             complement(<1..569)
                     /locus_tag="VGB93_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146494.1"
                     /translation="MARTYPSRWQQIGLELPLEPQFSREDFLISNSNADALRMIEAWP
                     HWPESVLLLIGERGSGKSHLAAIWAAQARAEIFSGASLAGADVAALAEMSALVIDAVD
                     EIGAAEAPLFHLLNLARQGRRSLLITAQRRPDLWGLKTPDLLSRLRLAPVVELGAPDD
                     ALLQSVLGKLFRDRQLLVDPSVIDFIALH"
     gene            complement(570..1712)
                     /locus_tag="VGB93_10185"
     CDS             complement(570..1712)
                     /locus_tag="VGB93_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0146495.1"
                     /translation="MRIEWQIVFWAAALAFIGMLLWLFNGVIMPFAAAFVIAYLMDPL
                     ADYLQRRGMSRLAATLFILFGIVFVLALILVLVVPVLAHQSVAFIQALPGLLNKLQSL
                     VSGVAGKLNSQYAEPVLRKLGYHSPSVPEMRASVGDIAGQAAGWFVGVLNSLVAHGAA
                     LISLMSLVVVTPVVAFYMLVDYHRMIAAVDGLIPPRYRGIVHQLAHQIDRTMSGFLRG
                     QSLVCLFLGLWYGLGLSLVGLNFGLLIGITAGFLSFIPYVGSMTALVAAAIIAIVQGW
                     PHWTLFAEAIGVVVVGQVLEGNVISPKLVGDNVGLHPVWLMFALLAFGSLFGFTGLII
                     AVPVAAATGVIMRFAVQKYRESHLFMNLPQGQTLILPETVNAPKEH"
     gene            complement(1745..>2144)
                     /locus_tag="VGB93_10190"
     CDS             complement(1745..>2144)
                     /locus_tag="VGB93_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0146496.1"
                     /translation="GWLAKTFNLRTELGAYLDPVADKALLVSIYVALSVYGAVPSWLT
                     IAVVARDVMIIGALIISWLMNKPVAIRPLWISKANTAAQIAFAAAVLGAKAFQFTLGP
                     WFIVSAVAVAALTLASLAAYLALWARHMSL"
BASE COUNT          537 a          647 c          628 g          332 t
ORIGIN      
        1 atatgcaagg cgatgaaatc gatgacggaa ggatcaacca gcaattgccg atcccggaaa
       61 agttttccga gtaccgattg aagcagcgcg tcgtctggcg cgcccagttc gacgaccggc
      121 gcaagccgca ggcgcgagag aagatctggc gtcttgaggc cccagagatc ggggcggcgc
      181 tgtgccgtta ttagcaggct tcgcctgccc tgccgcgcaa gattgaggag atgaaacaag
      241 ggcgcttcgg cggcgccaat ttcatctaca gcgtcgatca ccagtgccga catttctgcc
      301 agcgccgcaa cgtccgcacc cgccagcgac gctccggaaa aaatctccgc ccgcgcctgc
      361 gcagcccata ttgccgcgag atgacttttg ccgctgcccc gctcaccgat gagcagcaaa
      421 acagactccg gccaatgcgg ccacgcctcg atcatacgca gcgcgtcggc attggaattc
      481 gagatcagaa aatcttcgcg cgaaaactgc ggttcgagcg gcagttcgag gccgatctgc
      541 tgccagcgcg aaggataggt ccgcgccatt cagtgctctt tgggcgcgtt taccgtctcc
      601 ggcaggatga gcgtctgccc ttgtggcagg ttcatgaaaa gatggctttc gcgatatttc
      661 tgcacggcga agcgcatgat gacgccggtc gcagcggcaa ccggcacggc gatgatgagg
      721 ccggtgaacc cgaacagact accgaaggcg agcaaggcaa acatcagcca gaccggatgc
      781 agaccaacat tatcgccgac gagcttgggc gaaataacat ttccttcgag aacttgcccg
      841 acgacgacaa cgccgattgc ctctgcaaac aaggtccagt gcggccagcc ttgaacgatt
      901 gcaataatcg ccgccgcgac caaagccgtc atcgaaccga catagggaat gaagcttaag
      961 aaccctgcgg tgatgccaat cagcaggccg aagttcaagc cgaccagcga cagaccgagg
     1021 ccgtaccaaa gtccaagaaa tagacagacc agcgattgcc cacgcagaaa gccggacatc
     1081 gtccggtcga tctgatgtgc aagctgatgc acgatgccac gatagcgcgg tgggatgagg
     1141 ccatcgaccg cagcgatcat gcgatgataa tcaaccagca tgtagaaggc gaccactggc
     1201 gtcacgacga caagcgacat caggctgatg agagccgcgc cgtgcgcgac gagcgaattg
     1261 agaacgccga cgaaccaccc cgccgcctgc cctgcaatat cgccgacgct cgcccgcatt
     1321 tctggaacgc tcgggctatg atagccgagc ttgcgcagca caggctccgc atattgcgag
     1381 ttgagtttgc cagccacgcc ggaaactaga ctctgcaatt tgttcaacag gccgggcaaa
     1441 gcctggatga atgccactga ctgatgcgcc agcaccggaa caactaaaac caggatcagc
     1501 gcgaggacga agacgatacc gaagagaatg aagagcgtcg ctgcaagccg gctcatcccc
     1561 cgtcgctgca gataatcagc gagcgggtcc atcagatatg caatgacgaa ggcagcagcg
     1621 aacggcatga tgacgccgtt gaacagccac agcagcatgc cgatgaaggc gagcgccgca
     1681 gcccagaaca cgatttgcca ctcgatccgc atcaggtgac ctcaccgcat cttaaagcaa
     1741 aatctcaaag gctcatgtgc cgggcccaga gcgccagata ggccgcgagc gaggccaagg
     1801 tcaatgccgc tacggcgact gcggagacga taaaccacgg cccaagcgtg aattgaaacg
     1861 ccttggcgcc gagcaccgcc gcggcgaaag cgatctgcgc cgccgtgttg gccttggata
     1921 tccacaaggg acgaatggcg acgggcttgt tcatcagcca ggaaatgatc agggcaccga
     1981 tgatcatgac atcgcgcgcg acgaccgcga tcgtcagcca cgatggcacc gcgccataga
     2041 ccgagagcgc cacgtaaatc gagacgagca aagccttatc ggcgacgggg tcgagatagg
     2101 cgccaagttc ggtgcgcaga ttgaaggtct tcgccagcca gcca
//
LOCUS       DASWOG010000494         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_12075,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000494 DASWOG010000000
VERSION     DASWOG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12075"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VGB93_10195"
     CDS             <1..1481
                     /locus_tag="VGB93_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0146497.1"
                     /translation="KVVKVAMDITAEKLKNAEFAGKLNAISRAQGVIEFTIDGEIITA
                     NANFLTALGYTLEEIQGKHHRMLVDAEYAQSADYRDFWRRLQSGEFVAAEFKRIGKGK
                     KEVWIQASYNPIFDLNNRVVKVVKFATDVTERVVAVKEIGAGLSRLADQDLTYEIARE
                     FNPSFENLRLDFNRSVENLRNALVQIARGTDSIGSGTRSITTASDDLSQRTERQAASL
                     EETAAALDEITAVVKKSAAEAIHARQVAAEADDDAKKGTIVVRQAVEAMNAIAKSAKQ
                     ISQIIGVIDEIAFQTNLLALNAGVEAARAGDAGRGFAVVASEVRALAQRSAEAAKEIK
                     SLISSSTAEVENGVALVAETGKSLERILAQVVDINGIIGNIASGAQEQSAGLQQVNTA
                     VNDMDRVTQQNAAMVEETAAASHALAEDTAQVEALIAQFHIETGTENAPVRYEPRKSA
                     APVKQPEKMPRVSNARPTKQASRGSLKAVVGGGTVSSDWEEF"
     gene            complement(1621..1743)
                     /locus_tag="VGB93_10200"
     CDS             complement(1621..1743)
                     /locus_tag="VGB93_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146498.1"
                     /translation="MASRDDPDRRRRGSTARVLRDVLACLYFVSRPDKCNGERK"
     gene            1765..>2692
                     /locus_tag="VGB93_10205"
     CDS             1765..>2692
                     /locus_tag="VGB93_10205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0146499.1"
                     /translation="MTALSILDLVRVTEDTDARGALDNARDLAAHAEKWGYRRIWVAE
                     HHNMAGIASAATSIVIAHIAAGTNTIRVGAGGIMLPNHAPYVIAEQFGTLARLFPGRI
                     DLGLGRAPGTDQLTLRALRHSPEAADNFPEDVLELQAYFAPAGPNQRIVAVPAAGTEV
                     PLWILGSSHFGAMLAAELGLPYAFASHFAPELLIPALEIYRSRFKPSEQLARPYAMVG
                     VNIIAAESDAVARRLATTQQMSFTNIFRGARGLSQPPIDDIETYWSPLEKAQATKMLA
                     RSIIGSPDTVRSGIAALSAETGADEIMIVSDVY"
BASE COUNT          574 a          831 c          800 g          487 t
ORIGIN      
        1 tcaaggtcgt caaagtcgcc atggatatca ctgcggaaaa actcaagaac gcggaatttg
       61 ccggcaaatt aaatgccatc tcgcgagcgc agggcgtaat cgaatttaca atcgacggcg
      121 agatcatcac tgcgaacgca aatttcctga cggctcttgg ctacactctg gaagaaatcc
      181 agggcaaaca tcaccgtatg ttggtcgatg cggagtacgc gcaatcggcg gattatcgcg
      241 atttctggcg gcggctccag tcgggcgagt tcgtcgcggc agaattcaag cgcatcggca
      301 agggtaagaa ggaagtttgg attcaagcgt cctacaatcc gattttcgat ttgaacaatc
      361 gcgtcgtgaa ggtcgtgaaa tttgcgaccg acgtgaccga gcgcgtcgtc gcggtcaagg
      421 aaatcggcgc aggcctctcc cgacttgctg accaagacct gacttacgag attgctcgcg
      481 aatttaatcc gtcgtttgaa aatctgcgat tggatttcaa tcgctcggtc gaaaacctca
      541 gaaatgcact ggtgcagatc gcgcgtggga ccgattcgat cggttccggc acgcgctcga
      601 tcacgaccgc gtcggatgac ctttcgcagc gcacggaacg ccaggccgcg agcctggagg
      661 aaactgctgc cgcgctcgat gagatcacgg cggtcgtcaa gaaatcggcc gctgaggcga
      721 tccacgcgcg tcaggttgcg gccgaggccg acgacgacgc gaagaagggt acgattgtcg
      781 tccgtcaggc cgtcgaggcg atgaatgcca ttgcaaaatc agccaagcag atcagccaga
      841 tcatcggcgt catcgacgaa attgcattcc agaccaacct tcttgcgctg aatgctggcg
      901 tcgaagccgc acgcgcaggc gatgccggcc gcggttttgc ggttgtcgca tccgaggtgc
      961 gcgcgctcgc ccaacgttcg gcggaagccg ctaaggaaat caagagcctc atttccagtt
     1021 cgaccgccga agtagaaaac ggcgtcgccc tggtggccga aacgggtaag tcactggagc
     1081 gaattctggc tcaggtcgtc gacatcaacg gcatcatcgg caatatcgcc tcgggcgcac
     1141 aggaacaatc cgccggcttg cagcaagtca atacggccgt aaacgatatg gatcgtgtga
     1201 cgcagcaaaa tgcggcgatg gtcgaagaaa cggcggccgc aagccacgcg ctcgcggaag
     1261 atacggcaca agttgaggcg ctcattgccc aatttcacat tgaaaccggc acagagaacg
     1321 cccctgtacg ctatgaaccg cgcaagagcg cagcgcccgt caaacagccc gaaaaaatgc
     1381 cgcgtgtcag caacgcgcga ccgacaaaac aagccagccg cggctcgctg aaagcggtcg
     1441 tgggcggtgg cactgtcagc tccgactggg aagagttctg agctttcact tacggctcga
     1501 cgcccgagcg gtccgtgggc aatctcgcgt gcgcgccgcg ccttgatggc ccccatcggc
     1561 attgctgcga gcccataagc ctcgccagag catgattgct gcgactatcg gcgcagcgct
     1621 tcatttacgc tcgccattgc acttgtccgg acggctcacg aaatagaggc aggcgagaac
     1681 gtcgcgtagc acgcgagcgg tcgagccgcg gcggcgccga tcgggatcgt cgcggctcgc
     1741 catcaccgtc catccggagc aacaatgacg gccctgtcca tcctcgacct tgtccgcgtc
     1801 accgaggaca ccgatgcgcg cggcgcgctc gacaacgccc gcgatctcgc agcccatgcc
     1861 gaaaaatggg gctatcgtcg catctgggtg gcggaacatc acaatatggc cggcattgcc
     1921 agcgccgcga cgtcgatcgt gatcgcccat atcgctgcgg ggacaaacac catccgggtc
     1981 ggcgcgggcg gcatcatgct cccaaaccac gcgccctatg tcatcgcgga acagtttgga
     2041 accttggcgc gactgtttcc gggccgcatc gatctcggcc tcggccgtgc tcctggcacc
     2101 gaccagctca ccttgcgcgc cctgcgccac tcgcccgaag cggccgataa tttccctgag
     2161 gacgtgcttg aattgcaggc ttacttcgcg cctgcgggtc cgaatcagcg gatcgtggcc
     2221 gtgccggcgg cggggacaga agttccgctg tggattctcg gatcgagtca tttcggtgca
     2281 atgctcgcag cagagctcgg cctgccctat gccttcgcct cgcacttcgc acccgaattg
     2341 ctgataccgg cattggaaat ctatcgcagc cgtttcaaac cctccgaaca gcttgcccgc
     2401 ccctacgcga tggtcggcgt caatatcatc gccgcggaaa gcgacgcggt ggcgcgacgg
     2461 ctcgccacga cgcagcagat gtccttcacc aacatcttcc gtggcgcgcg cggcttgagt
     2521 cagccgccga tcgacgacat cgagacttat tggtcgccct tggaaaaagc acaggcgacg
     2581 aagatgctgg cccgttcaat catcggctcg ccagatacgg tgcgttcggg catcgccgcg
     2641 ctcagcgccg agaccggcgc tgacgagatc atgatcgtct ccgacgtcta cg
//
LOCUS       DASWOG010000495         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_121697,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000495 DASWOG010000000
VERSION     DASWOG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121697"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VGB93_10210"
     CDS             complement(<1..142)
                     /locus_tag="VGB93_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0146500.1"
                     /translation="MRLLLVVAAALIDADSRVLIAQRPEGKELAGLWEFPGGKLHEGE
                     RPE"
     gene            complement(139..813)
                     /locus_tag="VGB93_10215"
     CDS             complement(139..813)
                     /locus_tag="VGB93_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0146501.1"
                     /translation="MSLLIFDCDGVLVDSEVIALDVLRQMLADLGKPMSLAECRQRFM
                     GRRTRDVLAEIEKELGHPIPADTGLRMKALLFERFRRELRPVAGVKDAIAHLPGPICV
                     ASSSPPERIALSLEVTGLATLFGDRVFSAEQVPEGKPAPDLFLFAAKSMGASARETIV
                     IEDSTPGVLAARRAGMAVVGFAGASHSDDALVTALHAVGADAILTSMADLPRAVEKLR
                     AGQAVA"
     gene            complement(810..1544)
                     /locus_tag="VGB93_10220"
     CDS             complement(810..1544)
                     /locus_tag="VGB93_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146502.1"
                     /translation="MSLAGDPKQLEVFIKRKAFRAATGEVREVLRDFTLTLDAGKIHA
                     LVGPSGYGKTTLLRIIIGLDRDFEGRVTLPPETKIGMVFQEPRLLPWRSVLDNLKLAA
                     PQANEADLVKIADDLGVGDHLRNFPGELSLGQARRVALARAFAIKPNLLVLDEPFVSL
                     DAVLARRLADELVALVEQTQVTTLLVTHDIDEAVRLADHIILLGHRPAEVVGNIDVTL
                     PRRAMTADYAAKTKAEIGAILAENAV"
     gene            complement(1513..2250)
                     /locus_tag="VGB93_10225"
     CDS             complement(1513..2250)
                     /locus_tag="VGB93_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0146503.1"
                     /translation="MLRLLSLLMLIGLWQAASMVADTRLLPPPLTVLAVMGREAHTRA
                     LYFNLATTLARVVVAFTLAMVLGTIFGILMGRNRTFDRLGDPWLVILLNLPALVIIVL
                     AYVWLGLTDVAAVLAVALNKFPNTVVTIREGARALDPGLEEMATVFAMPWQTRIQHVV
                     LPQLAPYIAAATRAGLSLVWKIVLVVELMGRSNGVGFEIGTAFQLFDVALLLAYALPF
                     VAIMLAIETFLVQPLENHVSRWRPKAA"
     gene            complement(2255..>3061)
                     /locus_tag="VGB93_10230"
     CDS             complement(2255..>3061)
                     /locus_tag="VGB93_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146504.1"
                     /translation="DVTELATTEAGKVAIMGGSADVILSDWLWVSRERSLGGKLKFYP
                     YSSTLGAVMVPPGSPIKTLADLKDKKLGVAGGPLDKSWLLLQAYAKNSDVDLDSDATI
                     LYGAPPLLLQESLQREDDATLNFWNFCAALETKGFRRLIGIEDVEKALGASGRVAMVG
                     YVFDETYAASHADLLHRFFAMTAQAQDILAHSPEDWKKIGEKIGVTDPAALEIYRKRY
                     LEGAAHRSVADEEADARKLYLVLAGIGGAKLVGPAADLDAGTFYKDAAGK"
BASE COUNT          646 a          963 c          938 g          514 t
ORIGIN      
        1 tttccgggcg ctcgccctca tgcagcttgc cgccggggaa ttcccaaagg cccgcaagct
       61 ccttgccttc agggcgttgc gcgatcagca cacggctgtc ggcatcgatg agcgcggcgg
      121 cgacgacaag cagcagcctc atgcgaccgc ctgaccggcg cgcagctttt cgaccgcgcg
      181 gggcaaatcc gccatgcttg tcagaatcgc atccgcgccc accgcgtgca aggctgtcac
      241 gagcgcatca tcggaatggc ttgcgccggc gaagccgacg acggccatgc cggcccggcg
      301 tgccgcgaga acacctggcg tggaatcctc gatgacgatc gtctcgcgtg ccgaggcacc
      361 catcgatttc gcagcgaaga ggaaaagatc gggcgcgggc ttgccttctg gcacctgttc
      421 agcgctgaag acgcgatcgc cgaacaaggt cgcgagcccg gtcacctcaa gcgagagcgc
      481 gatacgctcc ggcggtgagg acgaggcgac gcagatcggc cctggtaggt gagcgatcgc
      541 gtctttcacg cccgcgacgg gtcgcagttc gcggcgaaat cgctcgaaca gcaacgcctt
      601 catgcgtagg ccggtatcgg cagggatggg atgaccaagt tccttttcga tctcggcaag
      661 aacgtcccga gtgcgccgcc ccatgaaacg ctgacggcat tcggccagcg acatcggctt
      721 gccgagatcg gccaacatct gccgcaaaac atctaaggcg atgacttcgg aatcgacgag
      781 cacgccgtcg caatcgaaaa tgaggagact cataccgcgt tctccgccaa aatagcgccg
      841 atctcggctt tggtcttggc agcgtaatca gcggtcatcg cccggcgcgg caaggtcaca
      901 tcgatattgc cgacgacctc cgccggacga tggccgagta ggatgatgtg gtcggcgagg
      961 cgcacggcct cgtcgatatc atgcgtaacg agcagtgtcg tgacttgagt ctgctcaacg
     1021 agcgcgacaa gctcgtccgc gagccgtcgc gcaagtacgg catcgagcga aacaaacggc
     1081 tcatcgagca caagcagatt gggtttgatc gcgaaggccc gcgccagcgc cacacggcgc
     1141 gcttgaccga gggacaattc gccggggaag ttacgcaaat gatcgccgac gccgagatcg
     1201 tcggcgattt tcacaagatc ggcctcgttt gcctgcggcg cagcgagctt cagattgtcg
     1261 agcaccgagc gccaggggag cagacgcggc tcctgaaaca ccatgccgat cttcgtctcc
     1321 ggcggcagcg tcacccggcc ttcgaaatcg cgatcaaggc cgatgatgat gcgcaggagc
     1381 gtcgtcttgc catagccgga agggccgaca agcgcgtgga tcttgccggc atcgagcgtc
     1441 agcgtgaaat cgcgcagcac ttcgcgtacc tcgcctgtcg cggcgcggaa ggccttgcgc
     1501 ttgatgaaga cttcaagctg ctttgggtcg ccagcgagag acatgattct cgagaggttg
     1561 cacgaggaag gtttcaatcg ccagcatgat cgcgacgaaa ggcagcgcat aggcaagcag
     1621 cagcgcgacg tcgaaaagct ggaaggcagt gccgatctcg aagccgacgc cgttcgagcg
     1681 gcccatcaat tcgacgacga gcacgatctt ccagacgagc gagaggccag cgcgcgttgc
     1741 cgccgcgata tagggcgcga gctgcggcag cacgacatgc tggatgcgcg tctgccaggg
     1801 catggcgaac accgtcgcca tttcttcgag gcctggatca agcgcgcggg cgccttcgcg
     1861 gatcgtcacc accgtattcg gaaatttatt cagcgcgacg gcaagcaccg cagcaacgtc
     1921 ggtcaatccc agccagacgt aggcgaggac gatgatcacc agcgccggca ggttgaggag
     1981 gataacgagc catggatcgc cgagcctgtc gaacgtcctg ttccgcccca tcaagatgcc
     2041 gaaaatcgtg ccgagcacca tcgccagagt gaaggcaacg acaacgcgcg ccaacgtcgt
     2101 agcgagattg aaataaagcg cgcgcgtatg ggcttcacgg cccataacgg cgaggacagt
     2161 gagcggcggc ggcaaaagcc ttgtgtcggc gaccatcgag gccgcctgcc acaaccctat
     2221 cagcatcaga agcgacaaaa gcctcagcag cacgtcattt gcccgccgcg tctttataga
     2281 atgtcccggc gtcgaggtcc gcagccggcc cgacaagctt ggcaccgccg atcccggcga
     2341 gcacgagata aagcttgcgc gcatccgcct cttcatcggc gaccgaacga tgcgcggcgc
     2401 cctccagata gcgcttgcgg tagatttcaa gtgccgccgg atcggtgaca ccgatcttct
     2461 cgccgatctt tttccaatcc tccggcgaat gcgccagaat atcctgcgct tgtgcggtca
     2521 tcgcgaagaa acgatgcaaa aggtccgcat ggctcgcggc ataagtctcg tcgaacacat
     2581 agccgaccat cgcaacccgg ccgctggcgc cgagcgcctt ttcgacatcc tcgatgccga
     2641 tcaaccgcct gaagcccttg gtttcaagcg cggcgcagaa attccagaaa ttgagcgtcg
     2701 catcgtcctc acgttgcagg ctttcctgca aaagcagcgg cggcgccccg tagagaatcg
     2761 tcgcatccga atcgagatcg acatcggaat ttttggcata ggcctgaagc agcagccagc
     2821 tcttgtcgag cggcccaccg gcaacgccaa gcttcttgtc cttgagatcg gcgagcgtct
     2881 tgattggcga acctgggggc accatcaccg cgccgagcgt gctcgaatag ggatagaatt
     2941 tgagtttgcc gccaaggctg cgctcacgtg acacccacag ccagtcggag aggatcacgt
     3001 cggccgaacc gcccatgatc gcgaccttgc cggcttcggt cgtcgcaagt tccgtgacat
     3061 c
//
LOCUS       DASWOG010000496         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_250283,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000496 DASWOG010000000
VERSION     DASWOG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250283"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..1054
                     /locus_tag="VGB93_10235"
     CDS             623..1054
                     /locus_tag="VGB93_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0146505.1"
                     /translation="MDWHTPGEILKDLCELFADARFLMALRNRGFQKCKVTLDISKSK
                     ALPYSMIASFKNRALKKYWTKNDASGIRPDWIAKVRIILSRLDAATEPKQMDIPGFGF
                     HALTGDLIGRYAVSVSRNWRITFAFDGEDAIDVDMEDYHGR"
     gene            1044..1358
                     /locus_tag="VGB93_10240"
     CDS             1044..1358
                     /locus_tag="VGB93_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0146506.1"
                     /translation="MVAKTLRHIDLQPSHPGELLREIVIPATGKSKVEIAQLLGISRQ
                     SLYDILAERQSITPAMAVRVGKMFGDGPEVWLKMQAAHDLWQAARDLKDEVADIPTLH
                     AA"
     gene            1382..1654
                     /locus_tag="VGB93_10245"
     CDS             1382..1654
                     /locus_tag="VGB93_10245"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0146507.1"
                     /translation="MTEVCALLHKGNANYGISFPDFPGCISGGKTAEEAIARGREALA
                     FHVAGLIEDGPALPRVRAVEDLQSDPCLREDFEAGVVALVPVEFPR"
     gene            complement(1917..2010)
                     /locus_tag="VGB93_10250"
     tRNA            complement(1917..2010)
                     /locus_tag="VGB93_10250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1974..1976),aa:Ser,seq:gct)
     gene            complement(2077..2970)
                     /locus_tag="VGB93_10255"
     CDS             complement(2077..2970)
                     /locus_tag="VGB93_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0146508.1"
                     /translation="MTHANAAYANWFTSAPDGLRLHLRDYGSPLDPGLPVVCLPGLAR
                     NAADFAPLAEALAAGAAGRKRRVIAVDYRGRGESDYDPDWQHYDLRVEGADILSQLAA
                     LEIHEAIFVGTSRGGLHIMTLAAARPALARAAVLNDVGPVIELQGLTRIRQYLRHSAA
                     PNSLSDAVDYVRKAMSAQFTALSDADFERYARASFQKPDGSFGATFDPNLVKPLETLR
                     LDEPLPQSWGLFEALAKVPLLIIRGSNSDVLSQETFVEMARRHRNCAAYVVEGQGHPP
                     LLADDATITRIADFIARADTA"
BASE COUNT          637 a          906 c          926 g          639 t
ORIGIN      
        1 cacgccttcg ccgcggtcgt tgctaacgaa ctcaatgccg gcttcttcta gggccgtggt
       61 aattgcggct ttggcattct ctgagatttc acggcgctcg cgctcaaagt ccacgacggt
      121 cgacagccca tccgccgcca cgaagacctc gagggcacgt atgacgaggt ggtggtgtcg
      181 ctgacaccgc gacctggttt gaactatcgc cgccggctgc tgccgagccg cctgcctgta
      241 tagcccgacg ccagcggaag cccgccagcg gccgtctcca acacaccccc tgccacccag
      301 cgcctgtctc gagacaggcg ctggcggccg cctatacacc tctgctcgcc gcttcggcga
      361 gggccggccc cggcagcccc tggccagcgg gtccttccgg gggcctcggc ccgcacgggc
      421 tgaagctcgc ggtctatttt ccaatttatc ttttttctta gggggttccg tttccggaag
      481 ccgtggtcaa aaagtcaaag tgattacaat acattaagtt gagttctgaa aacggaaggc
      541 cccataaaaa actatgcggt ccgcaattga cggtctccgc gacggcgcaa gctcctcgga
      601 ttacgaaccg ggagcatcgg ccgtggactg gcatacccct ggagaaatct tgaaagatct
      661 atgcgaactc ttcgctgacg ccagattttt gatggcgttg cgtaatcggg gttttcagaa
      721 gtgtaaggta actcttgaca tctcgaaaag taaggcatta ccttacagca tgatcgcttc
      781 gttcaaaaat cgcgcgctca agaaatattg gacgaagaac gatgcgagcg gcatcaggcc
      841 ggactggata gcaaaggttc gaattatcct ttcgcgtctc gacgctgcga ctgaaccgaa
      901 acagatggac atccctggtt ttggctttca cgccctgacc ggcgatctta taggccgtta
      961 tgccgtttct gtttcgcgaa actggcgcat tacattcgct tttgacggcg aggatgcaat
     1021 cgacgtcgac atggaggatt atcatggtcg ctaaaacgct acgccacatt gatctccagc
     1081 cgtcccatcc cggcgagctt ttgcgcgaaa tcgtgattcc ggcgaccgga aagagcaagg
     1141 tggaaatcgc ccagcttctc ggcatctccc ggcagtccct ctatgacatt ttggctgaaa
     1201 ggcagtctat cacgcccgcc atggccgtcc gtgtaggcaa gatgtttggc gatgggcctg
     1261 aggtttggct caagatgcag gccgcgcatg atctctggca ggcggcccgc gatctcaagg
     1321 acgaagttgc cgatattcct acgcttcacg ctgcgtgaag gcagaaggcg gtggagcata
     1381 aatgaccgaa gtttgcgcat tgttgcataa gggcaacgcc aattacggca tttctttccc
     1441 ggattttcct gggtgcatat ccggcggcaa aacggccgag gaagcgatcg cccgtggccg
     1501 cgaggcgttg gcgtttcatg ttgcgggtct gatcgaggat gggccggcgt tgccgcgggt
     1561 ccgcgccgtc gaggatttgc aaagcgatcc ctgtctgaga gaagatttcg aggcgggagt
     1621 cgttgctctc gttcccgttg aatttcccag ataacccacg cgcatcagta tttcgatcga
     1681 cgatcggctg cttgaacgga tcgacgccgg gccaaggcca ccggcgagac ccgttcaagt
     1741 tgtctcccga aagcggccat gcagcgtctg gcgagctgag gatgtgctct actacgacgg
     1801 aagttcgttg gctgaccagg cgactcaaat cgcattttgc ttcggacgaa atttcgccgt
     1861 gcgaggctag cggagcaact tggcgatcga ccaaacaaca gcttatttag ccaacatggc
     1921 ggagacggag ggattcgaac cctcgatagg gctttacaac cctataacgg tttagcaaac
     1981 cgccgccttc agcctctcgg ccacatctcc gaaacgatgc acatatgccc gacgtccggc
     2041 cggatggcaa ggcgccaggc gccacggctt ccctgttcaa gccgtatcag ctctggcgat
     2101 gaagtcggcg atccgcgtga tcgtcgcgtc atcggcgagc agcggcggat ggccttggcc
     2161 ttcaacgaca taggccgcgc aattccggtg gcggcgcgcc atttcgacga aagtctcctg
     2221 tgacaggacg tccgaattgc tgccgcgaat gatcaacagg ggtaccttcg ccagcgcctc
     2281 gaacaaaccc caggattgtg gcagcggctc gtcgagccgc agtgtctcga gcggtttcac
     2341 caggttcgga tcgaacgtcg cgccaaagct gccatccggt ttctgaaacg aggcgcgtgc
     2401 gtaacgctcg aaatcggcat ctgagagcgc cgtaaactgc gcgctcatgg ccttgcgcac
     2461 ataatcgaca gcgtcggaca aggaattcgg cgccgccgaa tgacgcagat attggcggat
     2521 gcgtgtgagc ccttgcagtt cgatcaccgg gccgacatcg ttcagaacag cggcacgtgc
     2581 cagcgccggc cgggccgccg ccagagtcat aatgtgcagg ccgccgcgtg acgtaccgac
     2641 aaagatggcc tcgtgaattt caagagccgc caattgcgag agaatatccg cgccttcgac
     2701 tcggaggtcg tagtgctgcc agtctggatc gtaatcggat tcgccgcggc cgcgataatc
     2761 gaccgcgatg acgcggcgtt tccggccggc tgctcctgcg gccagagcct ccgccagcgg
     2821 ggcaaaatcg gccgcattgc gcgccaggcc gggcaggcag acgacgggca agcccgggtc
     2881 gagggggctg ccgtagtcgc gcaagtgcag ccgcaggccg tccggggcgc tggtaaacca
     2941 attggcgtat gccgcgttag catgggtcat gggggagccg ggcagtaacg ctttctttac
     3001 cgtgattgcg cggatacgga acttacctca atttttagcg atttacggaa catgagcaat
     3061 tttgacaaca atcgccgcct tcgagtcctc attgtggatg atgaatgg
//
LOCUS       DASWOG010000497         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_323944,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000497 DASWOG010000000
VERSION     DASWOG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323944"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2854)
                     /gene="dnaE"
                     /locus_tag="VGB93_10260"
     CDS             complement(<1..2854)
                     /gene="dnaE"
                     /locus_tag="VGB93_10260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0146509.1"
                     /translation="MAVPAPGFATVAATVGFVHLHVHSAFSLREGALSIARLAKLAIA
                     DDMPALAITDSNNLFGALEFSEKLAKEGVQPIIGIELTLDFGDGARLASRGGETAAGR
                     AEIVLLAQDETGYLNLMHLASQAWLLPEPGDLPHLPVAMLAARTAGLLALTSGPDGVL
                     DRCFAFGRPEAAEHRLDVLQACFGGRLYVELQRHGLSPEREAEPQLIDLAYRYSLPLI
                     ASNEPYFAGVGEYEAHDALLCIAEGTVIGAPERRQLSPEHRFKTRAEMALAFADLPEA
                     LTNSVEAALRCAYRPLTRKPILPRFSNVAGDGEDEAAELVHQAEAGLEARLGKHGTTH
                     GLSEADYRARLAFELDVIIKMLFPGYFLIVADFIKYAKQNGIPVGPGRGSGAGSLVAY
                     ALTITDLDPLRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIRYVRDRYGADRVAQI
                     ITFGSFLARGVMRNVGRVLEMPLGQVDKLAKLVPQNPAAPVTLKQAIESEPRLREAAE
                     AEPRVARMLKIAETLEGLYSNASTHAAGIVIGDRPLEELVPLYRDPKSDMPATQFNMK
                     WVEPAGLVKFDFLGLKTLTVLDKTVRLLMLRGIAIDLAEIPLDDAATYEMLRRGETIG
                     VFQVESAGMRKALVEMRADRFEDLIALVALYRPGPMANIPTYCAVKLGDEPANYIHPK
                     IESILKETFGVIIYQEQVMQIAQILSGYSLGEADLLRRAMGKKIKAEMDAQRSRFVGG
                     AVERGLAKQQANEIFDLLAKFADYGFNKSHAAAYALLAYQTAYFKANHPVEFLAASMT
                     LDKGSTDKLAEFSNEARRIGIKIEPPSINRSGVDFEVAANAGDSLSILYALSAIKGVG
                     DGQAAALVASRVARPFADLADFAERISARDLNKRMLECLVAAGAFDTLFADRARVAGA
                     IEPILALAHRREDERAAGQSGLFDALRPDPLAGVKVE"
     gene            complement(3381..>3813)
                     /locus_tag="VGB93_10265"
     CDS             complement(3381..>3813)
                     /locus_tag="VGB93_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146510.1"
                     /translation="LAPIQVRKMKRRAAEDLARRYLARVKMADHADKYPAQLSGGEQQ
                     RVAIARALCMSPKIMLFDEPTSALDPEAVKDVLDLIMTLTAEDRMTMLCVTHEMGFAR
                     QVADRVIFMAAGEIVEINRPDAFFTAPRHQRTKLFLSQILR"
BASE COUNT          816 a         1168 c         1103 g          726 t
ORIGIN      
        1 cttcgacctt gacgcctgcg agcggatcgg gacgcaaagc gtcaaagaga ccggattgac
       61 cggcggcgcg ttcgtcctcg cggcgatgcg ccaacgccaa aatcggttcg atcgcgccgg
      121 cgacccgcgc ccggtcggca aagagcgtat cgaaggcgcc ggccgcaaca aggcactcca
      181 acatacgttt gttcagatcg cgcgccgaaa tccgctcggc gaaatcggca agatcagcaa
      241 aaggccgcgc gacgcgcgac gcgacgagag ccgcggcctg gccgtcgccg acgcctttga
      301 ttgcagacag cgcatagagg atcgaaaggc tgtcgcccgc attcgccgcg acttcgaaat
      361 cgacaccgga gcgattgatc gatggtggct cgatcttgat gccgatgcgc cgcgcctcat
      421 tgctgaattc cgccagctta tcggtcgagc ctttgtccaa cgtcatcgac gcggcaagaa
      481 attcgaccgg atgattggct ttgaaatagg ccgtctggta ggcaagcagc gcataggccg
      541 ccgcatggct cttgttgaag ccgtaatcag cgaatttcgc gagcaggtcg aagatttcgt
      601 tggcttgctg tttggcaagg ccccgctcaa ccgcaccgcc aacaaaacgg ctgcgctgcg
      661 catccatttc cgccttgatc ttcttgccca tcgcgcggcg cagaaggtcg gcttcgccca
      721 gcgaatagcc ggacaatatt tgcgcgatct gcatcacctg ctcttgatag atgatgacgc
      781 caaacgtctc tttcagaatc gattcgatct tcggatgaat gtaattcgcc ggctcgtcgc
      841 cgagcttcac cgcgcaatag gtcgggatat tggccatggg acccggccga tagagcgcga
      901 caagcgcgat cagatcctcg aaccggtcgg cgcgcatttc gaccagcgcc ttgcgcatgc
      961 cggcgctttc aacctgaaac acgccgattg tttcgccgcg ccgcaacatc tcgtaggtcg
     1021 ccgcatcgtc gagcggaatt tcagcaaggt cgatagcaat gccgcgcaac atcaataacc
     1081 gcactgtctt atcgaggacg gtgagtgtct tgaggccgag aaagtcgaat tttaccagcc
     1141 ccgccggctc gacccatttc atattgaact gcgtcgccgg catatcggat ttcgggtcgc
     1201 gatagagcgg cacgagttct tcaagcggcc ggtcgccgat gacaatacct gcggcgtggg
     1261 tcgaggcatt ggaataaaga ccttccagtg tctcggcgat tttgagcatc cgcgcgacgc
     1321 gtggctcggc ctcggcggct tcgcgcaagc gcggctcgga ttcgatggcc tgtttcagag
     1381 tgactggcgc ggcggggttt tgcggcacaa gcttggcgag cttatccacc tgacccagcg
     1441 gcatttccag aacccggccg acattgcgca tgacgccacg tgccaggaac gaaccgaatg
     1501 tgatgatctg cgccacccgg tcggcgccat aacgatcgcg gacatagcgg atgacctcgt
     1561 cgcgtcgatc ctgacagaaa tcgatgtcga aatccggcat cgacacgcgc tctggattga
     1621 gaaagcgttc gaacagaaga ccgaagcgca acggatcgag atcggtaatc gtcaacgcat
     1681 aagcgacgag tgagccggcg ccggacccgc gccccggccc gaccggaatg ccgttttgct
     1741 tggcgtattt gatgaagtcc gcgacgatca ggaaatagcc cggaaacagc atcttgatga
     1801 tgacatcgag ctcgaaagcg agacgcgcgc gataatcggc ttcgctgagg ccatgcgtcg
     1861 ttccgtgttt gccgagccgc gcttccagtc ccgcctccgc ctgatgaacg agctcggcgg
     1921 cttcgtcttc gccgtcgccc gccacattgg agaagcgggg caggatcggc ttgcgggtca
     1981 gggggcgata ggcgcagcgc agcgctgcct cgacgctgtt cgtcaaagct tccggcaaat
     2041 cggcaaaagc cagagccatt tccgcccgtg tcttgaaccg atgctcgggc gaaagctgcc
     2101 ggcgctccgg cgcgccgatc acggtgcctt cggcgataca gaggagtgca tcatgcgcct
     2161 catattcacc gacgccagca aaatacggct cgttgcttgc gatgaggggc agagaatatc
     2221 gataggccag atcgatcagt tgcggttcag cttcgcgctc gggcgacaaa ccgtgacgct
     2281 gcaattcaac gtaaagccgg ccgccgaaac acgcctgcag aacgtcgagc cgatgttccg
     2341 cggcttcggg acgcccaaat gcaaagcagc gatcgaggac gccatcgggg ccgctcgtca
     2401 aggcaagtag ccccgccgtc cgcgcagcga gcatcgccac cggcaaatgc ggcagatcgc
     2461 caggctcggg aagcagccat gcctgcgagg ccagatgcat caggttaaga tagcccgtct
     2521 cgtcctgcgc caggagcacg atctcggccc tcccagccgc ggtttcgccg ccgcgagacg
     2581 cgagacgggc cccgtcgccg aaatccagcg tcagttcgat gccgatgatc ggctggacac
     2641 cctccttggc gagcttttcg gagaactcta gtgcgccaaa aagattgttg ctgtcggtga
     2701 tcgccagcgc cggcatatcg tcggcaatgg cgagcttggc caatctggca atcgaaagcg
     2761 ccccctcgcg tagggaaaag gcactatgga catgaagatg cacaaagcca acggtcgcgg
     2821 cgacggtcgc aaatcccgga gccgggaccg caattcccgg ttggcctaag ccgccagcac
     2881 catcggcgaa agaaacgtcc aaaattcgcc cccggcctga taatgtcggc ccattcgagc
     2941 ccacgacgac cacagcttaa tcgagcgcga tccgatccgc gcgcaccaac ggcgtctctc
     3001 aacaattttt caggccgcgc cgacgcttcg cgcgacgatt gcgatcagga cgaccaaaca
     3061 actcagggat ataagctccg cggcatcggc aagaaacgca aacatacgca agctccacaa
     3121 acgctttatg ttcgcggttt attccctttt tgttccttag tcaagacact gatggaaaat
     3181 cctctccccg aaatctctga taaatttgta ctatcatcga cttgtgtgga gtccccgctt
     3241 tgttcttgtt ctatcccatt gttaattatg gggaaatcac gaaagcttta aggaactttt
     3301 cagaaaatgt cacatctaaa tgagatggga ataggtccta gatctgcata gccattgcac
     3361 tgattgagcc atccacccgc tcagcgcaaa atctgactca ggaaaagctt cgtgcgctga
     3421 tgccgcggcg ccgtaaagaa cgcgtccggc cggttgatct cgacgatctc gcctgcggcc
     3481 atgaagatca cccggtccgc aacctgccgg gcaaatccca tttcgtgcgt cacgcagagc
     3541 atggtcatcc ggtcctcggc ggtcaatgtc atgatcaggt cgagcacgtc cttcactgct
     3601 tccggatcga gcgccgaagt cggctcgtcg aacagcatga tcttcgggct catgcaaagt
     3661 gcgcgggcga tcgccacacg ctgctgttca ccgcctgaga gctgcgccgg atatttgtcg
     3721 gcgtgatccg ccattttcac acgagccaga tagcgacgcg cgaggtcttc ggcggcacgg
     3781 cgcttcatct tgcgaacctg aatcggcgcg agc
//
LOCUS       DASWOG010000498         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_158223,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000498 DASWOG010000000
VERSION     DASWOG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158223"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1318
                     /locus_tag="VGB93_10270"
     CDS             5..1318
                     /locus_tag="VGB93_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0146511.1"
                     /translation="MTDQALTRLTLSERTAAAMGDVLAGRRRGFADALLFVGPAVIAS
                     IAYMDPGNFATNIQAGARYGYALLWVVVLANLIAMLFQALSAKLGIVTGRNLAELCHA
                     HFPKPIVMAMWIVSEIAAMATDLAEFLGGAIGLSLLLHLPLIVGMGITAIVTYAILLV
                     EKNGFRPMELIIGTLVATIALCYVAEMFIAHVAWGAAAFHSVVPSLPGAAALTIAVGI
                     IGATVMPHAIYLHSGLTQNRAPAKNNDARRRLVRFSNIEVVVALAAAGLVNMAMVMMA
                     AAAFHAGHSDVAEIETAYHTLSPLLGPAAAGIFLVSLLASGISSSAVGTMAGQMIMQG
                     FVGFRIPVWLRRLLTMIPAFIVVALGVNATRSLVLSQVVLSIALPVPMIALVWFTRRT
                     DIMGAFANGRLTDFAAIAGTIIILGLNIVLLLQTCGAHIPGLPSS"
BASE COUNT          257 a          485 c          492 g          400 t
ORIGIN      
        1 gaatatgact gaccaagccc tcacgcgtct gacattgtcg gaacgcaccg cggcagctat
       61 gggcgatgtc ctggcgggcc ggcggcgggg cttcgccgac gccctcctct tcgtgggacc
      121 ggcggtcatc gcctcgatcg cctatatgga ccccggcaat ttcgcgacca atattcaggc
      181 cggcgcgcgc tacggctatg cgctgctctg ggttgtggtg ttggcgaatc tcatcgccat
      241 gctgttccag gcgttgtcgg ccaagctcgg cattgtcacc ggccgcaacc tcgccgaatt
      301 gtgccacgcg cattttccga agccaattgt catggccatg tggatcgtga gcgagattgc
      361 ggccatggcg acggatcttg ccgaatttct cggtggcgcg atcggcctgt cgcttctgct
      421 gcatctgccg ctcatcgtcg gcatgggcat cacggcaatc gtgacctatg cgattttgct
      481 ggtcgagaag aatggcttcc ggccgatgga actcatcatt ggtacgctcg ttgcgaccat
      541 cgcgctttgt tatgtcgcgg aaatgtttat cgcccatgtc gcttggggcg cggcggcttt
      601 tcattccgtc gtcccaagct tgcctggcgc ggctgcgctc acaattgccg tcgggattat
      661 cggcgcgacg gtgatgcccc atgcgatcta tctgcactcc ggactgaccc agaatcgtgc
      721 gccggcaaaa aacaacgacg cgcgacgccg tctcgtgcgt ttctccaaca ttgaagtcgt
      781 ggtcgcgctt gcggccgctg gcctcgtcaa tatggcgatg gtgatgatgg cggcggcggc
      841 cttccatgcc ggccacagcg acgtcgccga aatcgagacc gcgtatcaca cgctgtcgcc
      901 tttgctcggc cccgcggcgg ccgggatatt tctcgtctcg ctgctcgcct ccggtatttc
      961 gagctctgcc gtcggcacga tggccgggca gatgatcatg caaggcttcg tcggtttccg
     1021 cattccggtc tggctgcgcc gtctgctgac gatgataccg gccttcatcg tcgtggcgct
     1081 tggcgtgaat gcgacacgat cgctggtact cagtcaggtc gtgttgtcga tcgcgctgcc
     1141 ggtgccgatg atcgcgctgg tttggttcac gcgtcgcacc gacatcatgg gggctttcgc
     1201 caatggccgt ttgaccgatt tcgcggccat tgccggtacg atcatcatcc tcggcttaaa
     1261 tattgtcctg ctgttgcaaa cttgtggcgc acatattccc ggattgccgt cgagttgatc
     1321 gcccgccttg gcgctgtgcc aacaggcttc ctcgtcatat ttagaagagt tcttgtatcg
     1381 ctttagaagt tttctcttct tgaggcgaat cgttgactgt gaaaaatcgc agccctaacg
     1441 atctattatg ggctcattat ttgcgcgggg gcgaaatgct ttttgcagac aaacgcctgt
     1501 tggcaggcgg taatcgtaga tgcggtgcgc ggtgcaagcg cgctgttcac gaaactggcg
     1561 ctgcgctcgg cacattgttg ctgcttggca cgagtgcatg ggcggattca tctttgcctg
     1621 cgccgccgcc gacg
//
LOCUS       DASWOG010000499         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_30474,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000499 DASWOG010000000
VERSION     DASWOG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30474"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VGB93_10275"
     CDS             complement(<1..1297)
                     /locus_tag="VGB93_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0146512.1"
                     /translation="MVPNSGQSDHKSGIFGLAVGSIGVVYGDIGTSPLYALRESVKHG
                     NAAHRITEQSVTGTVSLLLWALFFTVTAKYVLFLMRADNKGEGGTLSLMALAQTAFGR
                     RAAPIFFLGVAGAALFSGDAIITPAISVLSALEGLNVATPVFSDYVLPLTIVVLISIF
                     AVQRHGTARVATFFGPMMVIFFVVIGFLGAIHIPDAPRILLAFDPLVGIAFLFGHGLV
                     GFIVLGSVFLAVTGAEALYADMGHFGRKPIQLAWLFFVLPALTLNYLGQGALVLANHA
                     AVENPFYLLAPHWALLPLVIMATIATVIASQAVITGAFSLTRQAIQLGLLPRMDVQHK
                     SATQEGQIFIPKVNRILLVGVLLLVVLFKKSDSLASAYGIAVSGTMVVTTALAFVVVW
                     RMWKWPLWGAVAFVSIFLLVDFTFFAANLMKIVDGGWVPL"
     gene            1614..>2056
                     /locus_tag="VGB93_10280"
     CDS             1614..>2056
                     /locus_tag="VGB93_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007813695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0146513.1"
                     /translation="MQSSEAKLPVFEVLPQIEAALRNHASAVLVAPPGAGKTTIVPLA
                     LRESGWAADKKIIVLEPRRLAARAAAERMAALLGENVGETIGLRMRLVSRASAATRNE
                     VVTEGVFARMILDDPALAGVGAVIFDEYHERSLDADFGLALALDT"
BASE COUNT          466 a          604 c          647 g          339 t
ORIGIN      
        1 tcaagggcac ccacccgcca tcgacaatct tcatgaggtt cgcggcaaag aaggtgaaat
       61 cgaccaaaag gaaaatcgac acgaaggcga ctgcgcccca gagcggccat ttccacatgc
      121 gccagacgac gacgaaggca agcgccgtcg tcacgaccat cgtgcctgaa acggcaatgc
      181 cataggctga ggctaaagag tcggacttct tgaacaggac gacgaggaga agcacgccaa
      241 cgagcaggat gcggttgacc ttcgggatga agatctggcc ttcctgtgtt gcggacttgt
      301 gctggacatc catgcgcggc agcaggccga gctgaattgc ctgccgcgtc agcgagaagg
      361 cgccggtgat caccgcctgg cttgcgatga cggtggcaat cgtcgccatg atcaccagcg
      421 gcagaagcgc ccagtgcggt gccagtagat aaaacggatt ctcgaccgcc gcatgattgg
      481 cgagcacaag cgcgccctgg ccaaggtagt tcaaggtgag cgcgggcaga acgaagaaaa
      541 gccaagcgag ttgaatcggc ttgcggccga aatggcccat gtcggcataa agcgcttccg
      601 cgccagtgac cgcaagaaag actgatccga gaacgataaa gccgacgagg ccgtggccga
      661 ataaaaaggc gatgccgacc agagggtcaa aagcgagcag aatccgcggc gcgtcgggga
      721 tgtgtatggc gccgaggaat ccaatgacga caaaaaagat caccatcatc gggccgaaga
      781 aggtggcgac gcgcgccgta ccgtgccgct ggacggcaaa gatggaaatc agcacgacga
      841 tcgtgagcgg caggacatag tcgctgaaaa ccggcgttgc gacgttcaaa ccttcaagcg
      901 ctgacagaac cgaaatcgcc ggtgtgatga tcgcatcacc cgaaaagagc gctgcgccag
      961 cgacacccag aaagaaaatc ggcgcggcgc gccggccgaa agccgtttgc gccagcgcca
     1021 tcaacgagag cgtgccgcct tcgcccttgt tgtcggcacg catgaggaac agcacatatt
     1081 tggcggtgac ggtgaaaaac agcgcccaga gcagcagaga gacggtgccg gtcacgctct
     1141 gctcagtgat ccgatgggcg gcattgccgt gtttgacgga ttcacgcagc gcatagagcg
     1201 ggctcgtgcc gatgtcgcca tagacgacac cgatcgaacc gacggccaag ccgaagatcc
     1261 cgctcttatg atcagactgt ccgctattgg gaacgattgg cccgcagtcg gccgagctgg
     1321 cggccttgtc ttgcgcggtc gctaccattc aaaaaagccc tcaatccgga cgctggcatg
     1381 acgttacccg gataggggca agtcggatgc caaacggcgg agcgaccggt tggttgagca
     1441 tggtcggcgg cggcatatag cgttttcccc ccgcgacgag aagcggaatc gtcttcaatc
     1501 gcccaaacaa gcatcatcaa ctgctttcgc gttgtaaatg gtccacgttt ggcttccagc
     1561 ccttggaacc gtcggagctt gcccattggc gcatcgcctg ccaattcagg ggcatgcaaa
     1621 gttccgaagc caagcttccg gttttcgagg ttctgccgca gattgaggcg gcgcttcgca
     1681 atcacgcgtc cgccgtgctc gtggcgccgc cgggcgccgg caagacgacc atcgtcccgc
     1741 tcgccctgcg ggaaagcggt tgggccgccg ataaaaagat tatcgtgctt gagccgcggc
     1801 ggctcgccgc gcgggcggcc gccgaacgta tggcggcctt gctcggcgaa aacgtcggcg
     1861 aaacaattgg cctgcgcatg cggctggtct cgcgcgcttc cgccgcaacg cggaatgagg
     1921 tcgtcaccga aggcgtgttt gctcgcatga tcctcgatga tccagctctc gccggcgtcg
     1981 gcgcggtgat cttcgatgaa tatcacgagc gctcgctcga cgcggatttc ggtctagcgc
     2041 tggcgctcga tacgca
//
LOCUS       DASWOG010000500         6647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_12215,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000500 DASWOG010000000
VERSION     DASWOG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12215"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGB93_10285"
     CDS             complement(<1..305)
                     /locus_tag="VGB93_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146514.1"
                     /translation="MSDTHAKIAIITGGSRGLGRNTALAVAARGIDVIFTYRTNKAEA
                     EKVAAEIKASGRKALALQLDTGDSTSFPAFAETIAAALRDTWGRNHFDYLVNNAGHSA
                     "
     gene            463..1356
                     /locus_tag="VGB93_10290"
     CDS             463..1356
                     /locus_tag="VGB93_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017990791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0146515.1"
                     /translation="MSVALKEALLAYMAEKGGHDGPYPTEIEALTLMRSTTPVLPHPM
                     VYRPALCVIVQGAKQVMFGDRIFDYEEMQCLVISIALPAFGRVVRATPDKPLLALHLD
                     FDMGIMRDVMAALEPPPRPSGAEDFSVFVEDLREPLADCLARLIRLLATPKAIPILYP
                     AIMREICFWLLTGPHAGEVCKLVLPNSQTQRIARAIHMLRERFSRPLRIEELATASGM
                     SASSFHQHFRALTAMTPLQYQKQLRLLEARRLLIEGEINAAHAAYRVGYESASQFSRE
                     YARMFGQPPKRDVTEVRALSA"
     gene            complement(1337..1801)
                     /locus_tag="VGB93_10295"
     CDS             complement(1337..1801)
                     /locus_tag="VGB93_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146516.1"
                     /translation="MATLAASGRRATRFKILVCALVASAGLIAGRLSFVEPSQHAITA
                     PRRILATSERDVIVAAIAQTLKDATAATIAWPPLILTSREGISDYCATVKESDRDVGF
                     YAQLIFPHADPREKLSQVNFAIIAVADDLEARYLVSVACLRYGYGAIMRTAP"
     gene            complement(1954..2853)
                     /locus_tag="VGB93_10300"
                     /pseudo
     CDS             complement(1954..2853)
                     /locus_tag="VGB93_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3069..4574)
                     /locus_tag="VGB93_10305"
     CDS             complement(3069..4574)
                     /locus_tag="VGB93_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY0146517.1"
                     /translation="MTNSAKLLALALLGLAAAAQPLAAQDASVKHATSAFTPGETATP
                     NPLPSAVTTSHELKFADRTLKFTAKAGAIRLSDANNGTPVADIAYVAFLKDGEDPANR
                     PITFAFNGGPGAGSAWLDLGALGPWRLPLDAGALAPSASPRTVDNAETWLDFTDLVFI
                     DAPETGYSRTLNKDAAKSFQNVGGDINALAVVIRKWLVENHRIASPKFIAGESYGGFR
                     APKLARRLVDNEGIGVNGIIMISPVLDFGWFESTNNPLTFATHLPSFTAAARNITHLE
                     GRGKLADVEAYAAGPYIVDLLRGEADAAALARISEKVAVFTGLDPALVRKLGGRIDTA
                     TFERERDRAQGRVASAYDATVTGFDPHPHAARSRYSDPVLDALKAPLASAMADIAANK
                     LNWFIDARYEILNESVSENWDWGHGNAEALDDLKEDLALDPHFRVLVAHGATDLVTPY
                     FTSKLLLDQIPPMGEPNRVRLVVYGGGHMLYARDKSRAALRDDARKLIEGK"
     gene            complement(4756..6321)
                     /locus_tag="VGB93_10310"
                     /pseudo
     CDS             complement(4756..6321)
                     /locus_tag="VGB93_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
BASE COUNT         1380 a         1891 c         1983 g         1393 t
ORIGIN      
        1 gcgctgtggc cggcattatt gacgagataa tcgaaatggt tgcggcccca ggtgtcgcgc
       61 agcgcggcag cgatagtctc ggcgaaggcc ggaaaactgg tgctgtcgcc cgtgtccaat
      121 tgcagcgcca gagccttgcg gcctgatgct ttgatctcgg cggcgacctt ttcagcctcg
      181 gccttgttgg tgcggtaggt gaagatcacg tcgatgccgc gtgccgcgac ggcaagcgcc
      241 gtattgcggc cgaggccgcg gctgccgccg gtgatgattg cgattttcgc gtgtgtatca
      301 gacatggctt tttgctccct ctgtctgagg tgcatatggg cgggatacgg cgcgccgtca
      361 ttgccggaag gcccgaaaag cttgcctgat cctccaaacc cgcttggaag cgccacgcgg
      421 tgtctctatt ctcctcacag aagaactcag ggtgtcgatc cgatgtctgt tgctttgaaa
      481 gaggccttac ttgcctacat ggccgaaaag ggcggccatg atgggccgta tccgaccgaa
      541 atcgaagctc tgactttgat gcgcagcacg acgccggtct tgccacatcc aatggtctac
      601 cgtccggcgc tctgcgtcat cgttcagggt gctaaacagg tcatgtttgg cgaccgaatc
      661 ttcgattacg aggagatgca atgccttgtg atcagcattg ctctgccggc cttcggccgc
      721 gtcgtgcgtg cgacgccaga caagccgttg cttgcgttgc atctcgattt cgacatgggc
      781 atcatgcggg acgtcatggc cgcgttggaa ccgccgccgc ggccaagcgg cgcggaggat
      841 ttcagcgtct ttgtcgaaga cttgcgcgag cccctggcgg attgccttgc acgccttatc
      901 cgactcctcg cgacgccgaa agccattccg attctatatc ccgcgatcat gcgcgagatc
      961 tgcttttggc tgcttaccgg gccacacgcg ggcgaagtct gtaagctcgt cctgccgaac
     1021 agtcagacgc agcgcattgc ccgggcaatc catatgctgc gagagcgatt ttccaggcct
     1081 cttcgcatcg aggagcttgc tactgcgtct ggaatgagcg cgtcgtcgtt tcatcagcat
     1141 ttcagggcgc tcaccgcgat gacgccgctg caataccaaa agcaattgcg tctgctcgaa
     1201 gcacgccgtc tgctgatcga gggcgagatc aatgccgccc atgccgccta tcgcgtcggc
     1261 tacgaaagcg catcccaatt cagccgcgaa tacgcgcgca tgttcgggca gccgccaaag
     1321 cgcgacgtga ccgaggtcag ggcgctgtcc gcatgatggc gccgtagcca taacgaagac
     1381 aagcgacgct cacgaggtaa cgtgcctcaa gatcgtccgc gactgcgata atcgcaaaat
     1441 tcacctggct caatttttcg cgcgggtcgg catgtggaaa tatgagctgg gcgtagaagc
     1501 cgacatcgcg gtcggattcc ttgaccgtcg cacaatagtc gctgatgcct tcacggcttg
     1561 tcaggatcag cggcggccat gcaatcgtcg cggccgttgc atcctttaat gtttgtgcaa
     1621 tcgccgcgac gatgacgtcc ctttccgagg ttgccaatat gcgccgcggc gcggtgatcg
     1681 cgtgttgtga tggttccacg aacgaaagcc gcccggcgat cagcccggcg ctcgcgacaa
     1741 gagcgcagac cagaattttg aagcgcgtag cacgacgccc cgatgcggcg agcgtggcca
     1801 tcccaatgac gtcctaatga aaatcgattt attaaatttc gagggagttt atagttgtac
     1861 cgcaagcgca aatacagcgg ctaatctcat ttatgtaaaa attaatactg aacgctgcat
     1921 cagcccctat ctcgccagtc gtttcgaaac gagctattgc cgccgtccgt aagcccggga
     1981 aaaagatcgt gtaattaatc ccacgtgctc gctcggggct gttgcccacg ggcgaggagt
     2041 tttcttcgcc ctcaaccctt tcaagaattc ggaatagacc atggcccaat tggaccggcg
     2101 ccgggtcaga ggcgagaagt cgatcgggtc ggctaacaca gtctccggga cgccatactt
     2161 cttgcagaaa taagcgatgt tgccgctgga atcgcgtccg ccactgccaa agagcgtatc
     2221 gtgggggatg tgaatgcaat aagcctcgaa atcgcagatc ggaaaaacat cggcgagaaa
     2281 gagccggaag aggaagtcga gatcatcgat cccataggtt ccgttatagc cttcgtcgaa
     2341 gccgccgatc gccttccaca gcgcgcgtga gacgcaatgg ccgccgtgtc ctctctgcca
     2401 tgcgggcaga tagccgtcag cgtcgagacc ctgttgttcg gcaccgtacc cccacatgaa
     2461 attgacaggg ccggcgcgat ctccagtgcc gtattcgaaa cgcggcccca tactgacgat
     2521 atttcggtgc cggacatgct gctgaagatg gcgctcgata tagaccgggc tcaaaacgac
     2581 atcgccatcg atcaatatca cgagaccgtc gagcgccgcc tcgaatccca gattccggat
     2641 cgtgttcagc cggtagtttt catcaggcgg tagcgcggta acgatgtgag ccaagctgaa
     2701 ttctcggcat ttgttgagaa agatatcgac ggattcatcc gtcgaggcgt cgtcggcaag
     2761 cacgaccaga tctgggcggc gggtctgctt tgcaattcgc tctaggacga cggaaagaac
     2821 gggcgcttta tttttgcagg ttatgacgat cgaagcacga ttagggtcag ttcaattccg
     2881 cgaactgtgg ccgccatttc tcggccagct ccgcgagcgc cttaaaatca gcaagcaaag
     2941 caggacgttg ctgctcaagt gcctccgcat tcccgcgtca ccccgttcgg catccttctt
     3001 ctttataggc atacaatcta agcgtgcaat tggattcggc gctggtctca ggccccaacg
     3061 caaagagcct attttccttc gatcaacttg cgggcgtcat cgcggagcgc cgcacgggat
     3121 ttgtcgcgcg cataaagcat atggccgccg ccgtagacga cgagccggac acggttcggc
     3181 tcacccatcg gcggaatctg atcgagcaaa agttttgagg tgaagtaggg cgtcacgaga
     3241 tcggtcgcgc catgcgcgac aagcacgcga aaatgcggat cgagcgcaag atcttccttc
     3301 aaatcgtcga gcgcctcggc attgccgtgg ccccaatccc aattttcgct gacgctttca
     3361 ttcaggattt catagcgcgc gtcgataaac cagttgagct tgtttgcggc gatatccgcc
     3421 atggcgcttg cgagcggcgc tttcaacgcg tcgagaaccg gatcggaata gcggctgcgc
     3481 gccgcatgtg gatgcgggtc aaagccggtg acggtcgcat cataggcgct ggcgacacgc
     3541 ccctgcgcgc gatcacgctc gcgttcaaag gttgcggtgt cgatccggcc accgagcttg
     3601 cggacgagcg ccgggtcgag accggtgaac acggcaactt tttcgctgat acgcgcgagc
     3661 gccgctgcgt cagcctcgcc gcgcaggaga tcgacgatgt aaggcccagc cgcataagcc
     3721 tcgacatccg ccaacttccc gcgtccctca agatgggtga tgttacgcgc ggcggcggta
     3781 aacgagggca ggtgggtcgc gaaggtcagt ggattgttgg tgctctcgaa ccagccgaaa
     3841 tccagcaccg gggagatcat gatgatcccg ttgacgccga tgccttcgtt gtcaacgagc
     3901 cggcgtgcga gctttggcgc gcgaaatccg ccatagcttt cgccggcaat gaatttcgga
     3961 ctcgcaatgc gatgattttc gacgagccac ttgcgaatga caacggccag cgcattgatg
     4021 tcgccgccga cgttctgaaa gcttttcgcg gcgtccttat tgagcgtgcg gctatagccg
     4081 gtttccggcg catcaatgaa aacgaggtcg gtgaagtcga gccaggtttc ggcattgtcg
     4141 accgtgcgtg gcgatgccga aggcgcgagt gcgccggcat cgagaggtaa acgccagggg
     4201 ccaagcgcac cgagatcaag ccaggccgag ccggcgccgg ggccgccatt gaaggcgaat
     4261 gtgatcgggc gatttgcggg gtcttcacca tccttcaaaa acgcgacata ggcaatatcc
     4321 gcgacaggcg taccgttgtt ggcatcgctt agccggattg cgccggcttt ggcggtgaac
     4381 ttgagtgtcc gatcggcgaa tttcagctca tgcgaggttg tcacggcgga cggcagcgga
     4441 ttaggcgtcg ccgtttcgcc gggtgtaaaa gccgaggtgg catgtttgac gctggcgtcc
     4501 tgcgccgcca gcggctgtgc ggcggcagcc aaacccaaca gagccaatgc caaaagcttt
     4561 gctgaattgg tcatgctgaa aatctccgcg cgcacccgga gggcataggc ccgcggaata
     4621 tggtcgcggt gaggcgtgcg gcataaagcg ggatgcgaaa agtgggttcc ggtttttcgc
     4681 gtagatcccg ctctaaatta tgagaatcga tcacgttcat gattttggat cgattcgatc
     4741 caaaatcatc gtgatctatg cggcgctgcg ggcttggcgc gacgcagacg ggtctggcgg
     4801 caggggctcg gcgggctcgt ccagatcgag ctgcgggagt gcgtccatca cgcgttcagg
     4861 cgggaagatc acaatgacct ccgtcccctc gcggacgcgc gacttcagcg taaacgtacc
     4921 gccgtgcaat tcaacgaggc ctttaacgat gggaagcccg agacccgaac cttcttcggc
     4981 atttttctgc gccagcgtgc cacgtccgaa cgacgacata acgatcggaa tttcatcttc
     5041 cggaattccc ggcccggtgt ccttgaccga gaaatattgg ccgccggttg aggtccagcc
     5101 gactttgatt gtcaccgttc caccctgagg tgtaaatttc acggcgttcg acagaaggtt
     5161 gatcgcaacc tgacggacgg cgcgctgatc cgcccagata tgcggcaatc ccgcttcgga
     5221 agattcggtg atcgtgatgt cgcgcttctt ggcccgcaaa gcgattagat ggcgacattc
     5281 gtccatgacg gcattgagcg cgaccggctc ggccttcaat tcatagcggc cggcctcaac
     5341 gcgcgagaga tcgagaatct cattgatcaa tgtcaggaga tgttggccgc tcgaatgaat
     5401 gtcctttgaa tattccttgt aggatgggac tttgtgggcg ccgaacaact ccgcctgcat
     5461 gacttcggag aagccgagaa tggcgttgag cggtgtgcgc agctcgtggc tcatcgtggc
     5521 gaggaagcgc gacttcgcca gattggcttc ctcagcgcgc cggcgcgcaa cgtcgctgtt
     5581 gatcttggcc tgttcgagtt cagcgatcaa ttcgtctttt tccgcctcga aggagagcga
     5641 gtcgatcgag cgtgcataaa gttttttggc gaggaccgcc gaataaagca atgtgctgca
     5701 tgccagcaca gccaggggaa ttgtgcggtc gtccaaactc gccggccata agaaggcgac
     5761 ggcagccgcc gtcatcggcg cgaggccggc ctagacggcg accggaatcg aagcggtgat
     5821 cgtcgcgttc atcgcagcga cgagcagcaa catgacaagc acgaatgtgc gggcgttggg
     5881 atcggcggta tcgccaatca atgacaccaa ttgtgcccag acgatgccct ggatcacctc
     5941 ggcgataacg aaattgccgc gccaattgcc aacattgatg cgcgccggct caagcgaaag
     6001 aaatttcccg gcaagccaat acagaacggc ggtcgtgatc agatcgaggc caacccatgc
     6061 aaccagtcgc gcgggcgtgg cccaagcaag ggcaaccagc gccgcgacga gtccgtaaag
     6121 cataaacgcg agcgtcgagc ccttacgcgc cttggcgtag agctggagca attcgacatc
     6181 gaacgcacga tggcccgagc ccgcagaggt aagcttttca cgcgcttcgc ggacttttgc
     6241 agccagccgt cgccgttcgg tcgcgacctt cgtgtcggcg ccgcgtcctc gctctatcat
     6301 ctcggcggtg acttcggtca tccttggcaa aatccgtcga tttgtagaac cgcgcgcaag
     6361 ttttcgttag agtgtgatca ggaaagatta acggtcgcct catgaattcg ttaagatcga
     6421 gtcaatctgg ccgtcctaag atcgttaatg tctgctgtgt catcccgcac atcgtgcatt
     6481 tcaaatattg gttaaatata tggttcagtc gagttacccg tgccggaggg gcgcgaaaaa
     6541 gcgtaagcgc gttcgcgagt taatgtctca acttcctgga cgaagcgatg tcgttttttt
     6601 cgaagcttgt tgcaaatttc catgttgatt ccggcgatga tgagcaa
//
LOCUS       DASWOG010000501         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_66731,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000501 DASWOG010000000
VERSION     DASWOG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66731"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGB93_10315"
     CDS             complement(<1..351)
                     /locus_tag="VGB93_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEY0146518.1"
                     /translation="MPLTDTRLRAIKPTAKLVKYSDSEGLQVWSYPNGAKLWRVAYRF
                     DNKQKLLAVGPYPLISLSDARQARDDAKRLLLQGRDPSAVKRELKAAKAAAGDTFEAI
                     GREYVEKLRREGRAE"
     gene            complement(531..606)
                     /locus_tag="VGB93_10320"
     tRNA            complement(531..606)
                     /locus_tag="VGB93_10320"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(571..573),aa:Phe,seq:gaa)
     gene            826..1248
                     /gene="ndk"
                     /locus_tag="VGB93_10325"
     CDS             826..1248
                     /gene="ndk"
                     /locus_tag="VGB93_10325"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY0146519.1"
                     /translation="MAIERTFSIIKPDATRRNLTGAINALIEAAGLRIVAQKRVLMTQ
                     AQAETFYAVHKARPFFGELVETMTAGPVVVQVLDGEDAIKKYREVLGATDPSRAADGT
                     VRKQFALSVGENSAHGSDAAETAALEIAQFFSGNEIIG"
     gene            1334..2029
                     /locus_tag="VGB93_10330"
     CDS             1334..2029
                     /locus_tag="VGB93_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEY0146520.1"
                     /translation="MQRRARGVLASFLSLGLALAISGPAAADKIKHSIAVFSGLDKIT
                     GRIISFEVSANETVQFGSLLVTDRVCYTRPPTEAPQTDTFVQVDEVDANKNTNRIFSG
                     WMFAASPGLNALDHPIYDIWLTDCKGTAQVIASAPSTAATPEPEAAPAPQQTPQNSDA
                     EPPPAPAPVKPPRHARHSAQQPPQDMQNGPVDVSPPLGFQAPPDNGGDDDQAPPPQVP
                     GMLPPDNNNNNNN"
BASE COUNT          404 a          640 c          601 g          389 t
ORIGIN      
        1 ctccgcccga ccttcgcggc ggagcttttc gacatactcg cgcccgatgg cctcgaacgt
       61 gtcgcccgcc gccgcctttg cggccttcaa ttcgcgtttg actgcggacg gatcgcgacc
      121 ttgcaatagg agacgcttcg catcatcgcg agcctgtcga gcgtctgaca gcgatatgag
      181 cggataggga ccaaccgcca agagcttttg cttgttgtcg aacctgtagg cgacgcgcca
      241 gagtttcgcg ccgttgggat atgaccacac ctgtagccct tcgctgtcgc tgtattttac
      301 gagcttggca gtgggcttga tcgcccgcaa tctcgtgtca gtcaggggca tttcgattcc
      361 agcgaatcct gtgtcagcta ccaacacaaa ctaatccgta ccgtcgacct taccaacgaa
      421 tttgttggat gatggcgctc ggatttggat tatggcggag gaccggcccg atcgaatgcg
      481 ctgaattatt agcagctttt gcgatatttg ggcggatttg caagagtttg tggtgcccag
      541 gggcggaatc gaaccaccga cactgcgatt ttcagtcgca tgctctacca actgagctac
      601 ctgggcaggt tccggccgac gccggaggcg cgctttataa gaagcgatgc cgggcttgtc
      661 cagcggcatg gcgtcttcaa ctcaaggcgc gcggcggggc cggccggcgc ttcatcccgg
      721 tcgtttgtga cgaaggcgtt tcgagattgc agcgtcgccg ccaccttgcc gcgcgccaaa
      781 cgcgccgcta taagcgcgcg gcccacctgc gagacattaa aaaacatggc gatcgaacgc
      841 accttttcca ttatcaagcc tgacgcaacg cgccgcaacc tcaccggcgc catcaatgcc
      901 ctgatcgaag cggcgggatt gcgcatcgtc gcccagaagc gcgtgttgat gacacaagcg
      961 caggctgaga cgttttatgc ggttcataag gcgcggccat tttttggcga gttggtcgag
     1021 acgatgaccg ccggcccggt ggtcgtgcag gttctcgatg gcgaagacgc gatcaagaaa
     1081 tatcgcgaag tgcttggcgc gacggatccc tcgagggctg cggatggcac ggtgcgcaag
     1141 cagttcgcgc tttcggttgg tgaaaattcg gcacatggat cggacgcggc agaaaccgcc
     1201 gcgcttgaaa tcgcgcagtt cttttccggc aacgagatca tcggctaacc ggcgcggccc
     1261 tattccggcc ggagtttgat gctcctttcg ccgcccacca agacgacgaa cagcgccgcg
     1321 gaccgagaac cttatgcaga gaagagcgcg cggcgttctg gcgtcatttc tgagccttgg
     1381 actcgcgctc gcgatctcgg ggccggccgc ggcggacaag atcaagcatt cgatcgcggt
     1441 cttttccggt ctcgataaaa tcaccggccg catcatctcg tttgaggtca gcgccaacga
     1501 aacggtgcag ttcggctcgc tgctcgtcac cgatcgcgtc tgctacactc ggccgccgac
     1561 cgaagcgccg cagacggata cgttcgttca agtcgatgag gtcgatgcca acaaaaacac
     1621 caaccgcatc ttttccggct ggatgtttgc ggcgagcccg ggtctcaacg cgctcgacca
     1681 tccgatctat gatatctggc tgaccgattg caaaggcaca gcgcaggtca tcgcctcggc
     1741 gccttcgacg gctgcgacgc cggagccgga ggccgcgccg gcgccacagc aaacgccgca
     1801 aaattccgac gctgagcctc cgccagcacc cgcaccggtc aaaccgccgc ggcacgcccg
     1861 gcatagcgcg cagcaaccgc cgcaggacat gcaaaacgga ccggttgacg tcagcccgcc
     1921 gctcggcttc caggcgcccc cggacaatgg cggtgatgac gaccaagccc cgccgccgca
     1981 agtgcccggc atgctgccgc ccgacaacaa taacaataac aacaactagg tttc
//
LOCUS       DASWOG010000502         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_176889,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000502 DASWOG010000000
VERSION     DASWOG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176889"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="coaE"
                     /locus_tag="VGB93_10335"
     CDS             complement(<1..440)
                     /gene="coaE"
                     /locus_tag="VGB93_10335"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873253.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0146521.1"
                     /translation="MFVLGLTGSIGMGKSVTAAIFREAGIPVHDSDAAVHALYRAAAV
                     PLVVAAFPGTLRDGEIDRTRLSAYVVNDPAALARLEAIVHPLVRAERAEFAAEARASG
                     ADLIVLDIPLLFETGCEGEVDAVLLVTAPEDVQKARVAGRPGMT"
     gene            complement(440..1279)
                     /locus_tag="VGB93_10340"
     CDS             complement(440..1279)
                     /locus_tag="VGB93_10340"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0146522.1"
                     /translation="MTLQAFVIGWPISHSRSPLIHNYWLEKLQVPGRYERFAVAPPDL
                     PTFLANLADLGFVGGNVTLPHKEKAFAACAVTTLVARQLQAVNTLWIEDEKLFGDNTD
                     VAGFLASLDADVPDWDKSVDKAVVLGAGGAARGIVFALNARGIRNIVIVNRTRSRGEQ
                     LQRQFPDAQIDLADFDALPGLLRDADLLVNTTSLGMIGQPPLEIDLAPLPARAVVADI
                     VYAPLETALLRQASACGLRTSGGLGMLLHQAVPGFARWFGQTPQVTPDLRARVAADIA
                     DKS"
     gene            complement(1276..>1563)
                     /locus_tag="VGB93_10345"
     CDS             complement(1276..>1563)
                     /locus_tag="VGB93_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0146523.1"
                     /translation="RTHALQSAVCVARDGTVLFEAISTANMTCRLFSQDFIDSYIAAA
                     GDSLLTSVGAYQVEGLGVHLFDKIDGDQATILGLPLLSLLAFLREEGSLAA"
BASE COUNT          325 a          489 c          493 g          256 t
ORIGIN      
        1 tctgtcatgc ccggtcttcc ggcaacgcgc gccttttgca cgtcttccgg cgcggtgacg
       61 agcagcaccg catcgacttc accctcgcag ccagtttcaa acagaagcgg aatgtcgagg
      121 acgatgagat cggcgcccga ggcgcgcgcc tcggcggcga attcagcgcg ctcggcgcgc
      181 acgagcgggt ggacgatcgc ttcaagccgc gccagcgccg ccgggtcatt gaccacatag
      241 gccgagaggc gcgtccggtc gatctcgccg tcgcgcagcg tgcccggaaa agcggccacg
      301 acgagcggca cggcggcggc gcgatagagc gcatgtacgg cggcatccga atcgtgcacg
      361 gggattccag cttcgcgaaa aatcgcggcg gtcaccgatt tgcccatgcc gatcgagccg
      421 gtgaggccga gaacgaacat cagcttttat ctgcgatgtc ggcggcaaca cgcgcgcgta
      481 agtccggcgt cacctgtggc gtttgcccaa accagcgcgc aaaccccggc actgcctgat
      541 gcagcaacat gccgagacca cccgatgtgc gcagcccgca cgctgacgcc tggcgcagaa
      601 gtgcagtttc aagcggtgca tagacgatat cggcgacgac ggcgcgtgct ggcaggggtg
      661 ccagatcgat ctcgagcggc ggctggccga tcatgccgag cgatgtcgta ttcaccagca
      721 aatcggcgtc gcgcagaagg ccgggcagcg cgtcgaaatc agcgaggtcg atctgcgcat
      781 caggaaattg cctttgcaat tgttcgcccc ggctgcgcgt gcgattgacg ataacgatgt
      841 tacggatgcc acgcgcgttc agcgcaaaga cgatcccgcg cgccgcgccg ccggcgccca
      901 ggacaacggc tttatccacg ctcttatccc aatccggaac atcagcgtcg agcgaggcaa
      961 gaaagcccgc gacatcggtg ttgtcgccga aaagtttttc atcctcgatc cacagggtat
     1021 tcacagcctg gagctggcgc gcgaccagcg tcgtcacggc gcaggctgcg aaggcttttt
     1081 ctttatgcgg cagcgtgaca ttgccgccga cgaatccgag atccgcaaga tttgccagaa
     1141 aggtcgggag atcgggcggg gccacggcaa accgctcata gcgacccggc acttgcagtt
     1201 tttccagcca gtaattgtgg atcagcggcg aacgcgaatg cgagatcggc cagccgatga
     1261 cgaaagcctg caaagtcatg ccgcgagact gccttcttcg cgcagaaatg caagcagcga
     1321 cagtagcggc aggccaagga tcgttgcctg atcgccatcg atcttgtcga agagatgcac
     1381 gccgaggcct tcgacctgat aggcgccgac gctggtcagc aacgaatctc cggccgcggc
     1441 aatgtaactg tcgataaagt cctgactgaa caaacggcag gtcatgttcg cggtcgagat
     1501 cgcctcgaaa agaacggtgc catcgcgcgc gacgcagacc gccgattgca gcgcatgcgt
     1561 ccg
//
LOCUS       DASWOG010000503        10512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_12266,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000503 DASWOG010000000
VERSION     DASWOG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12266"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VGB93_10350"
     CDS             complement(<1..1853)
                     /locus_tag="VGB93_10350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0146524.1"
                     /translation="MGALIWALPIGLGLGLSVANPPLVERLRSPVFDYDQQIQPRPYS
                     PNIPVRVVDIDDASLARLGQWPWPRHRLADLARHLFAQGAAVVDFDILFSEQDRSTPR
                     NLLAQLPDLPERKTLADALTAHGMMEDQSLEQVLTRGPSVLTLVLTNGVGTDVPVKSG
                     FVTLGDDPRPFLLNFRGAILPLAGLTETAAGLGSINWEPDRDLIVRKVPLISTQGDGA
                     AAHLVPSLDAEILRVAQHASTIIVKSSNASNTSGFGARTGVVGVKIGALQIAPESDGA
                     VRVHYSGTQAARHISAWRVLAGKVANSEIAGRIILIGSSASALADIRSTPLEAAVPGV
                     DIHAELLEHILSGTNLARPDYAPGLEALLLVVGGTIIAMMARFTKPVAAVSVLLLSLE
                     LLAFASFYAFSKMGLLFDPLMPGATWILAYAAMTVLVYRRSERQREFVRKAFSRYLAP
                     ALVERLAKDPSRLQLGGESRDVSVLFADVRDFTGRSEGLSAAEVVQFLNGVLTPLTQS
                     VLLEAGTIDKYFGDGLMAFWNAPLDVIDHAERACAAALAMREALPALNARLETGRSER
                     PIEIGVGINTGEAFVGNMGSDMRFDYSIVGDTVNVASRLEEATKELGVPIVV"
     gene            complement(1910..2686)
                     /gene="xth"
                     /locus_tag="VGB93_10355"
     CDS             complement(1910..2686)
                     /gene="xth"
                     /locus_tag="VGB93_10355"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0146525.1"
                     /translation="MQIVTWNVNSVRQRTEHLLRYLREIGPDVICLQELKCLDAAFPY
                     AEVEACGYNVAVHGQKGFNGVAILSKNPLEVTRGLPGDESDEHARYIEAVIPDGGGII
                     RVASIYLPNGNPPETDKYTHKLAWMDRLAAHARNLLELEEPLVLAGDFNVIPAARDCF
                     DPAVWAGDALYLPQTRARFHALLNLGFADALRATSDVGGLYTFWDYQAGAWQRNRGIR
                     IDHLLLSSRAADRLMQVTIDKERRGEEKPSDHVPVRIELC"
     gene            complement(2745..3986)
                     /locus_tag="VGB93_10360"
     CDS             complement(2745..3986)
                     /locus_tag="VGB93_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0146526.1"
                     /translation="MAEAESAVDILVVGAGAAGLCAALSLANSGHSVALAGPLEAASN
                     GRTVALFEGSLRFLRMQNLWPDLAGIAAKIIAIDLIDATNAPVPIPSLTFAADEIGLN
                     ALGANIENDKLVLRLADLVRRNPDIHRVEDLVADIEYHTDGATAVFASGRRIGAKLIV
                     AADGQRSTVRKEAGIGTRRWTYPQVALTVLLAHERPHHGRSVEFHTRSGPCTLVPLPP
                     RGEAQHRSSLVWLMSPQDAQRRRGLDADNLAAEVAHEAEEIYGEMRLDSDAGFFPMAG
                     MRAARLTGRRIALIAEAAHTFPPLAAQGLNLSLRDIATLTSSLETARQGREDVGGAAA
                     LHRYAAAQEPDIDIRVRGIDVLNRSMLSDALPIDMLRGLGFFAMSALGPLRRAALREG
                     VLPHRRRSLRSAPRPEPGAIF"
     gene            complement(3991..5307)
                     /gene="gltA"
                     /locus_tag="VGB93_10365"
     CDS             complement(3991..5307)
                     /gene="gltA"
                     /locus_tag="VGB93_10365"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0146527.1"
                     /translation="MLDAPKQASASDSAKFSIGDKTIDLPVKEGNIGPAVVDISKLYA
                     QTGMFTYDPGFTSTASCESEITYIDGDKGVLLYRGYPIEQLAEQGDFLETSYLLLYGE
                     LPTPAQRADWVHRITRHTMVHEQLARFFQGFRRDAHPMAVLVACVGALSAFYHDSTNI
                     SDPKQRMVASMRLIAKLPTLAAMAYKYSIGQPFVYPKNELDYSSNFLRMCFAVPCEDF
                     KVNPVMARALDQIFILHADHEQNASTSTVRLAGSSGANPFACVAAGVACLWGPAHGGA
                     NEAALKMLEEIGRVENIPTYIARAKDKNDSFRLMGFGHRVYKNYDPRAKLMQKTCHAV
                     LEAAGKKDDPLLDVAMELEQIALHDDYFIEKKLYPNIDFYSGITLKAMGFPTSMFTVL
                     FAVARTAGWVAQWKEMIEDPGQKIGRPRQLYTGARERPYVPIDERE"
     gene            complement(5459..6877)
                     /gene="gltX"
                     /locus_tag="VGB93_10370"
     CDS             complement(5459..6877)
                     /gene="gltX"
                     /locus_tag="VGB93_10370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0146528.1"
                     /translation="MAQVVTRFAPSPTGFLHIGGARTALFNWLYARHAGGKMLLRIED
                     TDRERSTDAAITAILDGLKWLGLDWDGDVVHQFQRAARHREIAEQLLAAGRAYHCYAT
                     PQELDDMRAKARAEGKPPRYDGRWRDRPASEAPAGVKPVVRLKAPQVGETVLDDLVQG
                     RVAWANENLDDFVLLRSDGTPTYMLAVVVDDHDMGITDIIRGDDHLTNAARQMQIYEA
                     LGWEVPRMAHIPLIHGADGAKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQ
                     EYFSTEEMIAAFDLGHVGRSAARFDFAKLENMNGHYIRTTDDETLVDLLIDTLPHLPE
                     NAAFPQNFDADMRQKLLAAMPGLKERAKTLTELRDGAIFLFAQRPLALDAAAAGILDA
                     SARAHLRGLLPDLATLETWNSTTTENVVRHYAEENHLKLGQVAQPLRAALTGRATSPG
                     IFDVFSVLGRDESLARLTDQSL"
     gene            7071..8606
                     /locus_tag="VGB93_10375"
     CDS             7071..8606
                     /locus_tag="VGB93_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0146529.1"
                     /translation="MRLVQFAGSARQAFQQAVEIEAAERRFFLWLPVAAGVGVILYLT
                     ADREPSLWYAAGAAIAFGALAAALRQHRHAQSVALVLCCICLGLFSASWRAARVAAPV
                     LDHIRVTTLEGMIEEMDFREQGARFLLRVDQADGLAAQATPYRVRLTLKRTPPFEAGS
                     YVRLKARLLPPAQESLPGGYDFARDAWFSRIGAVGNVLGRVTLMTPPHPSGLIDAALM
                     ALDRSRNALALRIDTIVGGEAGDVAAAMVKGKRDLLSDDTKEVIREAGIFHIITISGV
                     QMMLMAGIFFVGLRRVLALSPSLALRYPIKQWAAATAILGAIFYDIATGSRVGTERAL
                     FMTLIMLGAALAGRQSLSMRNLALAAVLVILVAPEAILGASFQLSFAAVAALVAVYES
                     RAENSAQIRDGRTGLVAPSPMRARIAALHTFFSHGPPGLLFATLCATTATASFMAYDF
                     HELNPYVLIGNPLTLTLIELFAVPCALLGTLLYPLGLDAFVWHYLGLGIRFVLTIIGR
                     RPS"
     gene            complement(9009..9722)
                     /gene="lexA"
                     /locus_tag="VGB93_10380"
     CDS             complement(9009..9722)
                     /gene="lexA"
                     /locus_tag="VGB93_10380"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0146530.1"
                     /translation="MLTRKQSELLRFINERLKETGVPPSFDEMKEALDLRSKSGIHRL
                     ILALEERGFIRRLPNRARALEVLRLPESTTPASNGRSRKFSPEVIEGNLGKVRPVMPA
                     FEDEDERAGMVAIPVMGRIAAGTPITALQSRSHTITLSPEMLSTGEHYALEVRGDSMI
                     DAGIFDADTVVIRKQDTAETGDIVVALIDDEEATLKRLRKRGASIALEAANSAYETRI
                     FGPDRVRIQGKLVSLIRKY"
BASE COUNT         1983 a         3202 c         3329 g         1998 t
ORIGIN      
        1 acgacgatcg ggacgcccaa ttctttcgtc gcctcttcga gacgcgaggc gacgttcacc
       61 gtgtcgccga cgattgaata atcgaaccgc atgtcggagc ccatgttgcc gacaaaggcc
      121 tcgccggtgt tgattccgac gccgatctcg attggccgtt ccgaacgtcc ggtttccagg
      181 cgcgcattga gcgcgggcag agcttcgcgc attgccaggg cggcagcaca agcacgttca
      241 gcgtgatcga taacatcgag cggtgcattc cagaatgcca tcagtccgtc gccgaaatat
      301 ttatcgatcg tgccggcttc gaggagaacg gattgcgtca gcggtgtcag cacgccgttg
      361 agaaattgca cgacttcggc ggcggagaga ccttcggaac ggccggtgaa gtcgcgcacg
      421 tcggcgaaga gtactgaaac gtcgcgcgac tcgccgccaa gctgcaacct gcttgggtcc
      481 tttgcgaggc gctcgacgag agccggtgcg agatagcgtg aaaaagcctt tcggacgaat
      541 tcgcgctgtc gctcgctgcg ccggtaaaca aggaccgtca tcgccgcata ggccaatatc
      601 catgttgcgc cgggcatcaa cggatcgaac agaaggccca tcttggagaa tgcgtagaag
      661 ctcgcgaagg caagcaattc gagcgacagc agcaaaacgc tgacagcagc gaccggcttg
      721 gtaaagcgcg ccatcatcgc gatgattgtg ccgccgacaa ccagcaggag agcttccaga
      781 cccggcgcgt aatccggccg cgccaaattt gtgcccgaga gaatatgctc caagagttcc
      841 gcgtggatat cgacaccggg gacggcggct tcgagcggcg tcgagcgaat atcggcaagc
      901 gcactggcgc tcgagccgat gaggataatg cggccggcga tctcgctatt cgcgactttt
      961 ccggcgagca cccgccacgc cgaaatgtgt cgcgccgctt gcgtgccgga ataatggacg
     1021 cgcaccgcgc cgtcggattc aggagcgatc tgcaacgcac cgattttaac accgacgact
     1081 ccggttctgg ctccgaaacc gctggtgttg gaggcgttcg aggacttcac gatgatcgtc
     1141 gaggcgtgtt gtgctacgcg aagaatttca gcatcgagcg aggggacgag atgcgctgct
     1201 gcgccgtcgc cttgcgtcga gataagcggg actttgcgga cgataagatc gcggtccggc
     1261 tcccagttaa tgctgccaag tccagcggct gtttcggtga gaccggccaa aggcaaaatc
     1321 gcaccgcgga aattgagcag aaacggccgt ggatcgtcgc ccaaagtgac gaagccggat
     1381 ttcactggca cgtcggtgcc gacgccgttg gtcagaacca gcgtaagcac acttggtcca
     1441 cgcgtgagca cctgttccag gctctgatct tccatcattc cgtgcgccgt cagtgcatca
     1501 gcgagcgtct tgcgttccgg taggtcgggc aattgcgcca gcagattgcg cggcgtcgaa
     1561 cgatcctgct cggagaagag aatatcgaag tcaaccaccg ccgcgccttg cgcaaaaaga
     1621 tgacgggcga gatcggcaag cctgtggcgc ggccaaggcc attggccgag tcgcgcgagc
     1681 gaagcatcgt caatatcaac gactctgacc ggtatgtttg gcgaataggg gcgcggctgg
     1741 atctgctgat cgtaatcgaa aacaggactg cgaagccgct cgacgagcgg tgggtttgcg
     1801 acggacagtc cgagtcccaa gccaatcggg agtgcccaga tcaacgcgcc caagatatac
     1861 gcggcgtagg gtcgaagccg cgccgcgagc ctccgagccg gcgaccgggt cagcaaagct
     1921 cgatcctgac ggggacatga tcggacggct tctcctcgcc gcgccgctcc ttatcgatcg
     1981 tgacttgcat caggcggtcg gcagcgcgcg acgacaacag cagatgatcg atgcggatgc
     2041 cgcgattgcg ctgccacgcg cccgcctggt aatcccaaaa tgtatagagc ccgcccacgt
     2101 cgctcgtcgc gcgcaacgca tcggcgaaac cgagattgag cagcgcgtgg aagcgcgccc
     2161 gcgtctgcgg cagatagagc gcgtcgcctg cccaaaccgc gggatcgaag caatcgcgag
     2221 ctgcggggat gacgttgaaa tccccagcga gaaccagcgg ttcttcaagc tcaagaagat
     2281 tgcgcgcatg cgcggcgaga cggtccatcc aggcgagctt gtgagtatat ttgtcggtct
     2341 caggcggatt gccgttggga aggtagattg aagcgacacg gatgattccg ccgccatccg
     2401 gaatcaccgc ctcgatatag cgcgcatgtt cgtcactctc gtcgcccggc aggccgcgcg
     2461 tgacctccag cgggttcttg gaaagaatag cgacgccgtt gaagcctttc tggccatgca
     2521 cggcgacatt gtagccgcag gcctcgacct ctgcataagg gaaggccgca tcgaggcatt
     2581 tcaattcctg caggcagatg acgtcagggc cgatttcgcg caaatagcgc agcaggtgtt
     2641 cggttctttg cctgaccgaa ttgacgttcc aggtgacaat ctgcatggcg gatcctgttt
     2701 cgggccgcca gtgtatcacg ggggccggcg acgcaaacca gccctcagaa gatcgcgccc
     2761 ggctcgggcc gcggggcaga tcgcaatgac cggcggcgat gcggcagaac accctcgcgc
     2821 agcgcggcac gacgcagcgg accgagcgcc gacatggcga agaagcccag cccccggagc
     2881 atatcgatgg gcagggcgtc ggagagcatc gagcggttca ggacgtcgat gccgcgaacg
     2941 cggatgtcga tgtcaggctc ctgcgccgcc gcataacgat gcagtgccgc cgcgccgcca
     3001 acatcctcgc gtccctggcg tgcggtctct aggcttgaag tgagcgttgc gatatcgcgc
     3061 aagctgaggt ttaggccctg cgctgcaagt ggcggaaagg tatgggccgc ctccgcgatc
     3121 aacgcgatcc ggcggccggt caaacgcgcg gcgcgcatcc ctgccatcgg aaagaatccg
     3181 gcgtcactat cgagccgcat ctcgccataa atctcctccg cctcatgggc gacctcggcg
     3241 gcgagattgt cggcgtcgag cccgcggcgc cgctgcgcgt cctgcggcga catcagccag
     3301 acaaggctcg agcgatgttg cgcttcgcca cgcggcggca acgggacgag agtgcagggg
     3361 ccgctgcggg tatggaattc gacggagcga ccgtggtgcg gccgttcgtg cgctagaagc
     3421 accgtgaggg caacttgcgg gtaggtccag cggcgcgtgc cgatgccggc ctctttgcgc
     3481 accgtcgaac gctggccatc ggcagcaacg atcagcttcg ccccgatccg gcgacccgaa
     3541 gcgaagaccg cggtggcgcc gtcagtatga tactcaatgt cggcgaccaa gtcctcgact
     3601 cggtgaatat ccggattgcg ccgcacgaga tcggccagac gcagaacgag cttatcgttt
     3661 tcgatattgg cgccgagcgc attgaggccg atttcatccg ccgcgaaggt cagcgatgga
     3721 attggcaccg gcgcattggt tgcgtcgatg agatcaatcg cgatgatttt cgcggcgatg
     3781 ccagcaaggt caggccagag attctgcatc cgcaaaaaac gcagcgaacc ctcgaagagg
     3841 gcgaccgtgc gcccattgct cgctgcttcg agcggcccgg cgagcgcgac ggagtggccg
     3901 gaattcgcga gagacagtgc cgcacaaagc ccggccgcgc cggcgccgac gacgagaata
     3961 tcgaccgcgg actccgcttc ggccacaatc ctactcgcgc tcgtcgatcg gcacgtaggg
     4021 gcgttcgcgc gcgccggtgt aaagctggcg cgggcggccg atcttctggc caggatcctc
     4081 gatcatttcc ttccactggg cgacccagcc ggcggtgcgc gccacggcga agagcacggt
     4141 gaacatggat gtcgggaagc ccatcgcctt cagcgtgatg cccgaataga aatcgatgtt
     4201 cggatagagc ttcttttcga tgaaatagtc gtcgtggagt gcgatctgct caagctccat
     4261 cgcgacatcg agcagcggat cgtctttctt gcccgccgct tcgagaaccg catgacaggt
     4321 cttctgcatg agcttggcgc gcggatcata gttcttatag acgcgatggc cgaagcccat
     4381 gagacggaac gaatcgttct tgtctttggc gcgggcgata tatgttggaa tgttctcgac
     4441 ccggccgatc tcctcaagca ttttgagcgc ggcttcgttg gcgccgccgt gcgctgggcc
     4501 ccagagacac gcgacgcccg ccgcgacaca ggcaaaggga ttggcgccgg aggagccggc
     4561 gagtcgcacc gtcgaggttg aagcgttctg ctcgtggtcg gcgtgcagga tgaaaatctg
     4621 gtcgagcgca cgggccatca ccggattgac tttgaagtcc tcgcagggaa ccgcgaaaca
     4681 catgcgcaag aagttcgacg aatagtcgag ttcgttcttc ggataaacga agggctgacc
     4741 gatcgaatat ttatacgcca tcgccgcgag cgtcgggagc ttcgcgatca gccgcatgga
     4801 cgcgaccatg cgctgcttcg gatcggagat attcgtcgag tcgtgataga aggctgagag
     4861 cgcgcccacg caggcaacga ggactgccat cggatgtgcg tcgcggcgga agccctggaa
     4921 gaagcgggcg agctgttcgt gcaccatcgt gtggcgcgtg atgcgatgga cccaatcggc
     4981 ccgctgcgcc ggtgtcggca actcgccata aagaagaagg taggacgttt cgaggaagtc
     5041 gccctgttcg gcgagctgtt cgatcggata gccgcgatag agcaggacac ccttatcgcc
     5101 gtcgatataa gtgatttccg actcgcagct tgcggtcgac gtgaagccgg gatcgtaggt
     5161 gaacatccca gtttgcgcgt aaagcttgct tatgtcgacg accgccggcc caatattgcc
     5221 ttccttgacc ggcaaatcga tcgtcttgtc gccgatcgag aatttggccg agtcgctcgc
     5281 ggaagcctgc ttgggtgcat cgagcatgaa agtctgcctt cttttgtgat tatcatccgt
     5341 gtgcgttgcc ggtccaaggg ctggccatgg gcgggcgccg ccagagcgga cttggtgcaa
     5401 tgcagtattg cctaccgcat agtcgagtcg tgttcaaggt cgcgtggtct gcggggcgtc
     5461 aaaggctttg atcggtcagc ctggcgaggc tttcgtcgcg tcccagaaca ctgaagacat
     5521 cgaaaatccc gggcgaggtt gcccgcccgg tcagcgctgc gcgtaaaggt tgggcaacct
     5581 gaccaagttt caagtgattt tcctcggcgt aatgccgcac gacgttttcc gtcgtcgtcg
     5641 aattccaggt ctcaagcgtc gccaagtccg gcaaaagccc acgcaaatgt gccctggccg
     5701 atgcatcgag gatgccagcc gccgcggcat cgagcgccag cggcctttgc gcgaacagga
     5761 agattgcgcc gtcgcgcagt tcggttagcg ttttcgcgcg ctccttgagg cccggcatcg
     5821 ccgcaagcag cttttgccgc atgtcagcat cgaagttttg cgggaaggcg gcattttccg
     5881 gcagatgcgg cagggtgtcg atcaaaagat cgacgagcgt ttcgtcatcc gtcgttcgga
     5941 tatagtggcc gttcatattt tcgagtttgg cgaaatcgaa ccgcgccgcc gaacgcccga
     6001 cgtggccgag atcgaacgcg gcgatcatct cctcggttga aaaatattcc tggtcgccgt
     6061 ggctccaacc aagccgcacg agataattgc gcaacgccgc cggcagataa cccatggcgc
     6121 gataggcatc gacgccgagc gcaccatgcc gtttcgacaa tttcgcgcca tcggcgccgt
     6181 ggatcagcgg gatatgcgcc atgcggggca cctcccagcc cagcgcctcg tatatctgca
     6241 tctgccgcgc tgcattggtg aggtgatcgt cgccgcggat gatatcggtg atgcccatgt
     6301 cgtgatcgtc gaccacgacg gcgagcatgt aggtaggagt cccatcggag cgcagcaaaa
     6361 cgaaatcgtc gagattttca ttcgcccagg cgacgcgccc ctgaacgaga tcgtcaagca
     6421 ccgtctcacc cacctgcggc gccttgagac gaaccaccgg tttgactccc gccggcgcct
     6481 cggacgcggg ccgatcacgc cagcgcccgt cgtagcgcgg cggcttgccc tcggcgcgcg
     6541 ccttggcgcg catatcgtcg agctcttgcg gcgtcgcata gcaatgatag gcgcgccctg
     6601 ccgcaagtaa ttgttcggcg atttcgcggt ggcgcgccgc gcgctggaac tgatgcacga
     6661 catcgccatc ccaatcgagg ccgagccatt tcagtccgtc gagaatggcc gtgatcgccg
     6721 cgtcggtcga acgttcgcga tccgtgtcct cgatccggag cagcattttg ccgcccgcgt
     6781 gacgcgcgta gagccagttg aacagcgccg tccgtgcccc gccaatatgc agaaacccgg
     6841 tcggcgaagg cgcaaaacgt gtcaccacct gagccatgag cgaagtctga tcctcttacc
     6901 cgtaatccga tcacaaagtg cggccctaag ggccgggcgc ggtttagcac ggacagggcg
     6961 aaagaaaagc ccgcggcctt gccacgcgcc ggtctcggat caatcatgcc cgctcggggg
     7021 agtgccccgg gacgaccttt gcgaacggct tcgcgccggc gcgcttcggc gtgcgcctcg
     7081 tgcaattcgc cgggtctgcg cggcaggcgt ttcagcaggc tgtcgaaatc gaggctgccg
     7141 agcggcggtt tttcctctgg ctcccggtcg ccgccggcgt cggcgttatc ctctatctca
     7201 ccgccgaccg ggaaccctcg ctctggtatg ccgccggcgc tgccatcgcc ttcggcgcgc
     7261 tggccgcagc gctgcgacag catcgccatg cgcagagcgt cgcgcttgtt ctctgttgca
     7321 tctgccttgg gctattttcg gcaagctggc gcgcggcacg ggtcgcggcg ccggtgctcg
     7381 atcatatccg cgtcacgacg ctcgaaggca tgatcgagga aatggatttt cgtgagcaag
     7441 gggcgcgctt tctactgcgc gtcgatcaag ccgatgggct tgcggcgcag gcaacaccct
     7501 atcgcgtgcg cctgacgttg aagcgcacgc cgccgtttga ggcgggcagc tatgttcggc
     7561 tcaaggcgcg gctgttgccg ccggcgcagg agagtttgcc cggcggctac gatttcgccc
     7621 gcgatgcgtg gttctcccgc atcggcgccg taggcaatgt gctcggccgc gtgactttga
     7681 tgacgccgcc acatccgtcg gggttgatcg atgccgcgct gatggcgctc gaccgcagcc
     7741 gcaacgcgct cgcgctgcgc atcgacacga ttgtcggtgg cgaggccggc gacgtcgccg
     7801 cggccatggt gaagggcaag cgcgatttgc tttcggatga cacgaaagag gtgatccgcg
     7861 aggcgggcat ctttcacatc atcacgattt ccggcgtgca gatgatgctg atggccggca
     7921 ttttcttcgt cggcctacgt cgtgtgttgg cgctctcgcc gtcgctggcg ctgcgctacc
     7981 ccatcaaaca atgggcggcg gcgacagcga tcctcggcgc gatcttctac gacatcgcga
     8041 cgggctcgcg cgtcggcacc gagcgcgcgt tgttcatgac cttgatcatg ctcggcgcgg
     8101 cgctcgccgg acggcaatcg ctcagcatgc gcaatctcgc gctcgccgct gtgctggtga
     8161 tacttgttgc gcccgaggcg atcctcggcg cgagctttca gctttccttc gccgcggtcg
     8221 ccgcacttgt cgcggtgtac gaatcccgcg cagaaaatag tgcgcaaatt cgcgacgggc
     8281 gcaccggcct cgtcgcgccg tcgccgatgc gcgcgcggat cgcggcactg cacacgttct
     8341 tcagtcacgg gccgccgggt ttgctgtttg cgactctatg cgcgacgacg gcaacggcct
     8401 cgttcatggc ctacgatttc catgagctca atccttacgt gctcatcggc aatccgctga
     8461 cgctgacatt gatcgaattg tttgccgtgc catgcgcgct tctcggcacg ctgctttatc
     8521 cgctcggact cgatgcgttc gtctggcatt atctcggcct cggcatccgg ttcgtgctga
     8581 cgatcattgg ccggcggccg agctagttca cggtcgagca atggcttggc gccgacgccg
     8641 atgggggcga cccgcgtcaa gccatcgaca cgagcgcctg tgaccgcatc ggctgcgtcg
     8701 gcactctggc cgatggccgc agcgttgcgc tggtgctgga cgacgcggct ttcgccgaag
     8761 actgcctgcg agccgatgtg atcatcacgc cgctctacgc gccgccgggc tgcgccgcga
     8821 cgctcgtcat cgatcgcgac aaactgcgcg gcagcggcgc aagcttttgc gcgcgcaagg
     8881 caagggctgg gcgttcgaca ccgctcgtgc cgtggacgaa gaccgacctt ggtcgccagc
     8941 gccgcctgcg tgggcgcgga cagccgcaac agcagctaag gtcaccgact cagacgcgcc
     9001 gtaagccttc aatatttcct gatgaggctc acgagcttgc cctggatgcg tacgcggtcg
     9061 ggcccgaaga tacgcgtctc ataggccgaa ttggcggctt caagcgcgat cgacgcgccg
     9121 cgcttgcgca accgcttcaa cgtcgcctcc tcgtcgtcga tcagcgcgac gacgatgtcg
     9181 ccggtctcgg ccgtgtcctg cttgcggatc acgaccgtgt cagcatcgaa aatgccggca
     9241 tcgatcatcg aatcgccgcg cacttcgagc gcgtaatgtt cgccggtcga gagcatctcc
     9301 ggggacaggg tgatcgtgtg gctgcggctc tgcagcgcgg tgatcggcgt gccggcggcg
     9361 atgcggccca tgaccggaat ggcgaccatg ccggcgcgct catcctcgtc ctcgaacgcg
     9421 ggcatgaccg ggcgcacctt gcccaagttg ccctcgataa cctcgggcga aaatttgcgg
     9481 ctgcgcccat tcgaggcggg cgtcgtcgac tcgggcaggc gcagcacttc aagcgctcgg
     9541 gcgcggttcg gcaggcggcg gatgaagccg cgctcctcga gcgccaagat caggcggtgg
     9601 atgccggatt tcgagcgcag atcgagcgct tccttcatct cgtcgaagga gggcgggaca
     9661 cccgtttcct tcaggcgctc gttgatgaag cgcagcagtt cgctttgttt acgggtcaac
     9721 attcaggcgc tctcgctctt gggcggctcc ggagccgaat cactccggtt cagaagacgg
     9781 gaagccgcta aacaaagagt gaacagaccc taaccgttct tgctgtgttc cgcaagggga
     9841 gggagccgcc tttttgagca tggttttaac cacactcaaa ttgccaggat atctgacaga
     9901 cctttagagc cgcggcgggc gcaagcgatt gctggaaatc ccagtgaccc gcagcagact
     9961 taagacatgg gtggtcccaa gcctcgaaga ttgtgggccg ctcgaaacac ttcctgagta
    10021 ggctatctgc ttggcggcgt ccgaaccatc ttcttcatgg cggtcgccgc ctcgtttgga
    10081 atctccgtgt cggtctttga cttaggtgat ttggacatgc agccgctcag tagagtcaaa
    10141 ccaacagcaa acgtcgtttt tggcgaggat gatacaatgc agcagcggcc tgagctggcc
    10201 gtcttagttg cagaatgtat aactcagttc gccaacctag attctatgat gggcctatct
    10261 ctagctctta ttctagacgc gaaccagaaa gccattctgc tatgtatagc gcattcgata
    10321 gccgcgcttc acaaatgaaa cttttgaaag cagccactga atctgagctt gagaagcctc
    10381 attgcgatat ttttgacgcg ataattactc ttttcgtcaa gcccgcaata agggatcggg
    10441 acaaatttgc tcactgggtg tggggatacg cacctgaatt gccagacgcc cttttgctta
    10501 tggagccgag ag
//
LOCUS       DASWOG010000504         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_341583,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000504 DASWOG010000000
VERSION     DASWOG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341583"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..420)
                     /locus_tag="VGB93_10385"
     CDS             complement(139..420)
                     /locus_tag="VGB93_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY0146531.1"
                     /translation="MMTKSTLAKATLASSLAAAVALVAVQAQAETKKSEKCFGVALKG
                     QNDCAAGAGTTCAGTQAIDYDGHYFRFVPKGTCTAIKTPKGTGSRTAKL"
     gene            621..1181
                     /locus_tag="VGB93_10390"
     CDS             621..1181
                     /locus_tag="VGB93_10390"
                     /inference="COORDINATES: protein motif:HMM:NF009165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0146532.1"
                     /translation="MRVDRNSEWSALLRAANAGDARAYQRFLQELAPVLRAFVRRLLM
                     RTSSGGTEAEDIVQEILLAIHLKRQTWIETAPVTPWVFTIARHKTIDALRRRGRHIDV
                     PIDDFTDILADENDEPNLASVYIDRELGTLPDGQRKVVQAIAVAGDSITEAAEKLKMT
                     QGAVRVALHRGLAALAARTTKRSGSA"
     gene            1178..1813
                     /locus_tag="VGB93_10395"
     CDS             1178..1813
                     /locus_tag="VGB93_10395"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEY0146533.1"
                     /translation="MKTDDLIRSLATDNGPRMSLDRTLLYGFLPGVAISLLIYAVRMG
                     MRPHIFDMIGEPRLLFKIAFPIAVFACAGPLALQLARPRGNPRPLVITLLALLAVLAV
                     AIVVELSVLPQGLWSVRLIGHNARFCMVMIPLLSAAPLIGALIALHRGAPRNPGLTGA
                     TAGLFAGAFAASLYATHCPDDSPLFVATWYTLAILVVMIVGALAGSRFLRW"
     gene            complement(1817..2350)
                     /locus_tag="VGB93_10400"
     CDS             complement(1817..2350)
                     /locus_tag="VGB93_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146534.1"
                     /translation="MPNERLLMIVEDDSAFANTLKRSFERRQYDVLLAESFADVSSLL
                     QTQTPGYAVVDLKLGGGSGLACVEALHAHDPDMLIVVLTGYASIATAVEAIKLGACHY
                     LAKPSNTDDIEEAFKKSAGNTEVPLTDRPTSIKTLEWEHIHRALVEADFNISETARRL
                     GMHRRTLARKLEKRQVK"
     gene            complement(2340..3740)
                     /locus_tag="VGB93_10405"
     CDS             complement(2340..3740)
                     /locus_tag="VGB93_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146535.1"
                     /translation="MSANAIDAGHMLAREDTQIAKPEFEATPHASSRAEPKTAPPISN
                     AANRKNMVLLIQFRWVAVVGQLVTITMVQYGLGIPLPLPAMLAVLAALILVNVASFIW
                     LRLHEDISSAALLLVSMLDVFALTVQLWLSGGATNPFVSLYLLQITMAAILLDAQSTW
                     LVVALAFTGFVVLVSFHQPLEMPRRWSGDMFSLHIFGMLVCFILNAALLVVFLTRITR
                     NLSERDARLAALRQSAIEEDHIVRIGLLATGAAHELGTPLASLSVILGDWRRMPLIKG
                     NAEMRQEVEEMQAAVNRCKAIVTDILLSAGEARGEAPSITTVTKFLDDIVASWREGRP
                     AAVLSYENKFGADLPIVSDSALKQIVFNVLDNAVEASPTWVGFTAMRDTDSLILEVSD
                     LGPGFAPEILANFGRPYQSSKGRAGRGLGLFLVVNVVRKLGGVAEARNRTGRGAIVTL
                     RLPLTALAIGAPHYAE"
     gene            complement(3721..4371)
                     /locus_tag="VGB93_10410"
     CDS             complement(3721..4371)
                     /locus_tag="VGB93_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY0146536.1"
                     /translation="MAGLTGLGVWQLERRVWKLGLIARVDARVHAAPVAPPGPADWPK
                     VSAERDEYLRVRAAGHFLNAHETDVQALTALGPGFWVLTPLKTDQGFIILVNRGFVQP
                     EQRAPAAHGAVNGETIVTGLLRMTEPKGRFLRPNDPAQDRWYSRDVAAIAATRELTGV
                     APYFIDAEAGADPEAWPRGGLTVLDFPNNHLLYALTWFSLALMLAGASLYNVRQRN"
     gene            complement(4443..4823)
                     /gene="cyoD"
                     /locus_tag="VGB93_10415"
     CDS             complement(4443..4823)
                     /gene="cyoD"
                     /locus_tag="VGB93_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY0146537.1"
                     /translation="MSHDAHAIDDDHGHDDAPPHGTLQSYLIGFGLSVVLTVIPFWLI
                     MSGVIANKAIAAITIMAFAAVQIVVHIIYFLHVNFRSERGWTFLTLMFTAIVLFITLS
                     GSLWVMFHLTSNMAPTPAVMRQMP"
     gene            complement(4820..5455)
                     /gene="cyoC"
                     /locus_tag="VGB93_10420"
     CDS             complement(4820..5455)
                     /gene="cyoC"
                     /locus_tag="VGB93_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEY0146538.1"
                     /translation="MTVASDTAAQGGQPPVYYVIDEHEHPEGGSTYLGFWVYVMSDCL
                     IFACLFATFGVLGGNYAAGPAPKDVFELPIVAVNTAMLLLSSITYGFAMLAMEKDKVG
                     ATLGWLAVTALFGASFVGIELHEFAGLIHEGATPERSAFLSSFFTLVGTHGLHVTSGL
                     VWMLVLMSQVATKGLIAANKRRLMCLSMFWHFLDVVWIGVFTFVYLLGMLR"
     gene            complement(5459..>6024)
                     /locus_tag="VGB93_10425"
     CDS             complement(5459..>6024)
                     /locus_tag="VGB93_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEY0146539.1"
                     /translation="LYVLGFMGVTRRLRVIDDPSLHNWFIIAFLGAVMIFLGIISFLI
                     QIAVSIKNREALTDTTGDPWNGRTLEWSTSSPPPSYNFAFTPVVHDIDAWWDMKQHGY
                     VRPLEGFKPIHMPKNTAAGIILAGFATAFGFAMIWHIWWLAIASFAAAIATGIAHTFN
                     YHRDFYIPAADVSLTEDERTRHLSAKV"
BASE COUNT         1198 a         1754 c         1946 g         1126 t
ORIGIN      
        1 cacggcaagg tcgtggagca gggcattgcg agcgagattt tcgccaatcc gcagagcgat
       61 tatacccggg ctctattcgc tgcggccttt gccatcgagg cggaccgcag cggcgtggtc
      121 gcgcaatagt acgtggcctc acaattttgc ggtgcgcgag ccggtgcctt tcggcgtctt
      181 gatcgcggtg caggtgccct tggggacgaa cctgaaataa tggccatcgt aatcgatcgc
      241 ctgcgtcccg gcgcaggtgg tgccggcgcc ggcggcgcaa tcgttctggc ccttcagcgc
      301 gacgccaaag catttctcgg actttttggt ttcggcctgc gcctgaacgg cgaccaacgc
      361 gacggctgcg gcaagtgaag aggccagggt cgccttggct agtgtggatt tggtcatcat
      421 gaatctgctc ccgagcctcg ataaatgggg gccgccttga agctcaagac ggtttgcaac
      481 ccgcactgct ccggtacggc cggccttcgc cgagcgttac ccccgaacgc ggcagatttt
      541 agatcacgaa ggcgtgacaa gacgtgggta aaaaggcatg taacgcccag ccgggctttt
      601 gcgaacgcca aagaagacgc gtgcgggtag accgaaacag cgagtggagc gctcttctac
      661 gcgccgccaa cgcgggggat gcccgggcct atcaacggtt cctgcaagaa ttggcgccgg
      721 tacttcgggc gtttgtgcgg cgtttgctga tgcgaacgag ttcggggggt actgaggctg
      781 aggatatcgt gcaggaaatc ttacttgcca ttcatttgaa acggcagacc tggatcgaaa
      841 cggcgccggt gacgccctgg gtcttcacga tcgcgcggca caagaccatc gacgccctgc
      901 ggcggcgtgg ccgccatatc gatgtgccga tcgacgattt caccgatatt ctggcggacg
      961 aaaacgacga gcctaatctc gccagcgtct acatcgaccg tgagcttgga acgctgcccg
     1021 atggccagcg gaaggtcgtg caggctatcg ccgtcgcggg cgattccatc accgaggcgg
     1081 cggagaagct gaagatgacg caaggcgcgg tgcgggtggc tctgcatcgc gggctggcag
     1141 cacttgcggc gcgcaccacc aagaggagcg gcagcgcatg aagacggacg atctgatccg
     1201 tagtcttgcc accgacaacg gcccgcgcat gtcgctcgac cggacgctac tctatggctt
     1261 cttaccgggt gttgcgattt ccctgctcat ctacgccgtg cggatgggta tgcgaccgca
     1321 tattttcgat atgattggcg aaccgcggct cctcttcaag atcgcttttc caatcgcggt
     1381 ttttgcctgt gccgggccgt tggctttgca attggcgcgg ccacgcggca atccgcggcc
     1441 gcttgtcatc acccttttgg cgctgctcgc tgtgcttgcc gtcgcaatcg tggtcgagct
     1501 ttccgttttg ccgcagggtc tttggtccgt tcggctcatc ggccataacg cgcgcttctg
     1561 tatggtgatg attccactgc tttcagccgc gcccttgatc ggcgccttga ttgccctgca
     1621 tcgcggcgcg ccgcggaatc ccggcctcac gggcgcaacc gccgggcttt ttgccggggc
     1681 ttttgccgcc tcgctttacg cgacccattg ccctgacgat tcaccgcttt tcgtcgccac
     1741 ctggtacacg cttgcgattc tcgtcgtgat gatcgtcggc gcgctggcgg gcagccgttt
     1801 cctgcgctgg tagtgtttat tttacctgcc gcttctcaag tttgcgggcg agggtgcgcc
     1861 gatgcatgcc gagccgacgc gcggtttccg aaatattgaa atcggcttcg accagcgcgc
     1921 ggtggatgtg ctcccactca agcgttttga tcgacgtcgg tcggtcggta agcggcacct
     1981 cggtattgcc ggccgatttc ttgaaggctt cttcaatgtc gtcggtattt gagggcttgg
     2041 cgagataatg gcaggcaccg agcttgatcg cctcgacggc cgtcgcaatg ctggcatagc
     2101 cggtgagcac gacgatcagc atgtccggat cgtgcgcatg cagagcttcg acgcaggcaa
     2161 ggccggagcc gccgccgagt ttcaggtcga cgaccgcata acccggcgtc tgcgtctgta
     2221 aaagcgaact gacgtccgcg aagctctcag cgagtagcac atcatactgg cgccgctcga
     2281 acgaacgctt gagcgtattg gcgaaagcgg aatcgtcctc gacgatcatc agcagacgct
     2341 cattcggcat aatgcggtgc tccaattgcg agggcggtca acggcaggcg taacgtgacg
     2401 atagcgcccc ggccggtgcg gttgcgcgcc tcggcgacac cgccgagttt cctgaccaca
     2461 ttcacgacga ggaaaagacc gagcccgcgg ccggcgcgac ccttgctcga ttgataggga
     2521 cggccaaaat tggcgagaat ctcgggcgcg aacccggggc cgagatcgct gacttcgagg
     2581 atgagcgaat ccgtgtcgcg catcgcggtg aagccaaccc aggtcggcga tgcttcgaca
     2641 gcattgtcga ggacattgaa aacgatctgc ttcagtgctg aatcggagac gatcggcaaa
     2701 tcggcgccga atttattctc ataggagagc accgccgcag gtctgccctc gcgccagctc
     2761 gcgacgatat cgtcgaggaa ttttgttacc gtggtgatac ttggagcttc accgcgcgcc
     2821 tcgccagccg aaagcaggat gtcggtgacg atcgccttgc agcgattgac cgctgcctgc
     2881 atctcctcga cctcctggcg catctcggca ttgcctttga tcagcggcat acgccgccaa
     2941 tcgccgagga tgacagagag agaggcgagc ggcgttccga gctcatgcgc cgcacccgtc
     3001 gcgagcaggc cgatacggac gatatgatcc tcttcgatcg cgctttgtct gagcgctgcg
     3061 agacgcgcat cgcgttcact caggttgcgc gtgatgcgcg tcaggaacac cacaagtagc
     3121 gccgcattga gaatgaagca gacgagcatc ccgaaaatat gcagcgagaa catgtcgccg
     3181 gaccagcggc gcggcatctc cagcggctgg tggaagctca cgagcacgac gaagccggtg
     3241 aaggcgaggg cgacgacgag ccaggtcgat tgcgcgtcca ataggatcgc cgccatcgtg
     3301 atctgcagca gataaagtga aacgaacggg ttggtggcgc cgccgctcag ccaaagttgg
     3361 accgtcagcg cgaaaacgtc gagcatcgac acgaggagca aggcggcgct gctgatatcc
     3421 tcatgcagac gcagccagat gaagcttgcg acattgacca agatcagcgc ggcgagaacg
     3481 gcgagcattg cgggcagcgg caggggaatg ccgagcccat attgcaccat cgtgatggtg
     3541 acgagctgcc cgactaccgc gacccagcga aactggatca gcagaaccat gttcttgcgg
     3601 ttggcggcgt ttgaaatcgg cggcgcggtt ttcggctccg cacggcttga tgcgtgcggc
     3661 gtcgcttcga attccggctt cgcgatctgg gtgtcttcgc gcgcgagcat gtgtccagca
     3721 tcaattgcgt tggcggacat tatagaggct tgccccggcc aacatcaaag ccaagctgaa
     3781 ccaggtcagc gcgtagagga ggtggttgtt ggggaagtcg agcacggtca ggccgccgcg
     3841 gggccaagct tctggatcgg cgccggcctc cgcatcgatg aaatagggcg cgacgccggt
     3901 cagctcacgg gtggcggcga tggcggcgac gtcgcgagaa taccatcggt cttgtgctgg
     3961 atcgttcggg cgtaaaaagc ggcctttcgg ctccgtcatt cgcaacaggc cggttacgat
     4021 ggtttcacca ttgaccgcgc catgcgccgc aggcgcgcgt tgctccggct gaacaaaacc
     4081 acgattgacg aggatgatga agccttggtc ggtcttgagc ggcgtcagaa cccaaaagcc
     4141 cggtccgagc gccgtcagag cctggacatc agtttcatgg gcgttgagga aatgtccggc
     4201 ggcgcgaacc cgcaggtatt cgtcgcgttc ggctgagact tttggccagt ccgcgggacc
     4261 tggcggcgca acgggcgcgg cgtgaacgcg cgcatcgaca cgggcgatga ggccgagttt
     4321 ccagacgcgg cgctcaagct gccagacacc gaggccggtg agcccggcga tgacgagaaa
     4381 tacgctggcg agaaccgcga accgcgcgag cggcgagcgt tgcccgccgc cgcccgtaat
     4441 tgtcacggca tctgtctcat gacggcgggg gtcggcgcca tattcgaggt cagatggaac
     4501 atgacccaca gtgagccgct gagcgtaatg aacaggacga tcgcggtgaa catgagcgtc
     4561 aggaacgtcc agccgcgctc ggacctaaag tttacatgca ggaaatagat gatgtggacg
     4621 acgatctgca cggcagcgaa cgccatgatc gtgatggccg cgatagcctt gttggcgatg
     4681 acgcccgaca tgatcagcca aaagggaatg accgtcagga cgaccgaaag gccgaagccg
     4741 atcagatagc tttgcagcgt cccatgcggg ggcgcgtcat catggccgtg atcgtcgtcg
     4801 atcgcgtgcg cgtcatggct catcgcaaca tccccagaag atagacaaag gtgaacacgc
     4861 cgatccagac gacgtcgaga aagtgccaga acatgctgag gcacatcagg cggcgcttgt
     4921 tggccgcgat caggcccttg gtcgcgacct gggacatgag caccagcatc cagacaaggc
     4981 cggaggtgac gtgcagaccg tgggtgccga caagggtgaa gaaagacgat aggaaggcgc
     5041 tgcgctccgg cgtcgcgccc tcgtggatca ggccggcgaa ctcgtgcaac tcgatgccga
     5101 cgaagcttgc gccgaagagc gctgtaacgg cgagccagcc taaagtggcg ccgaccttgt
     5161 ccttctccat cgccagcatg gcgaatccgt aggtgatcga ggagaggagt agcatcgcgg
     5221 tgttgaccgc cacgatgggc agttcgaaca catccttcgg tgccggaccg gcagcgtaat
     5281 tgccgccaag cacgccaaac gtcgcgaaca ggcacgcgaa gatgaggcaa tcgctcatca
     5341 catagaccca gaagcccaga taggtgcttc cgccctcggg atgctcgtgc tcgtcgatga
     5401 cgtaataaac gggcggttgc ccgccctggg cggcggtgtc gctcgcaacg gtcatggatt
     5461 aaaccttcgc cgagagatga cgggtgcgtt cgtcttccgt caggctgaca tcagccgccg
     5521 gaatatagaa gtcgcggtga tagttgaacg tgtgcgcgat acctgtcgcg atcgccgcgg
     5581 caaaacttgc gatcgccagc caccagatat gccagatcat cgcgaagccg aaggcggtgg
     5641 cgaagcccgc aaggatgata ccggcggctg tgttcttcgg catgtggatc ggcttgaacc
     5701 cttcgagcgg gcgaacgtag ccatgttgct tcatgtccca ccaggcgtcg atgtcatgca
     5761 cgacgggcgt gaaggcaaag ttataggagg gcggcggcga cgaggtcgac cattccagcg
     5821 tgcggccgtt ccacggatcg ccggtcgtgt cggtcaaagc ttcgcgattc ttgatgctga
     5881 cggcaatctg aatgaggaag ctaatgatgc cgaggaagat catcacggcg ccgaggaagg
     5941 cgatgatgaa ccagttgtgc agcgacggat cgtcaatcac acgcaggcgg cgcgtgacgc
     6001 ccatgaagcc gagtacatag agcg
//
LOCUS       DASWOG010000505         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_250506,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000505 DASWOG010000000
VERSION     DASWOG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250506"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGB93_10430"
     CDS             <1..358
                     /locus_tag="VGB93_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY0146540.1"
                     /translation="VTAFATGDRELLARLLDKDVNGSFASAIDARAARGESLMTKIVS
                     IDKTGVFDAGVRDGMLQITLRFMASLISATHDKDGKLIDGDPEKPVNMVDLWTFARPA
                     ASRDPNWKLVATQTGH"
     gene            460..1026
                     /locus_tag="VGB93_10435"
     CDS             460..1026
                     /locus_tag="VGB93_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0146541.1"
                     /translation="MSEETRETLRPLSHEEVTLWLTVTRHVLRRSGIKAPLAPPPKPE
                     KPVKSAPSPGPAQVRPEPPPRPAMPGLAPLERRARQKLSRGHIPVDAALDLHGLRQHE
                     AHPALHSFLIRAQHQGAKIVLVVTGKGEGRTGDVYEEGGVLRRNVPIWLRAPEWRNLV
                     VGFEEAARHHGGAGALYIRIRRRSRGED"
     gene            1023..1436
                     /locus_tag="VGB93_10440"
     CDS             1023..1436
                     /locus_tag="VGB93_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY0146542.1"
                     /translation="MIPINWPDLVPPSLADFETLADAAYARLPDDFRALCQGIIIRVD
                     DFPDDEVLDRMACETEFDLLGLFSGRGLAQGGAQSFTGQMPNMVWLYRRPILDYWAEN
                     EDTLGEIVTHVLVHEIGHHFGLSDADMEAIEAAGD"
     gene            1548..2093
                     /locus_tag="VGB93_10445"
     CDS             1548..2093
                     /locus_tag="VGB93_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146543.1"
                     /translation="MRRADVLRWAEDAIDQLQTLLVICVLDEGELEPAFEREKLAAIM
                     LTTSTLAERGRLFFKNEIRGDGHGQEKELAYQGYRPKILDQLVVAHQIARHWPQANAD
                     DRKIMLILAQDAARTFISLAQQEVGRSKTASAVTKRAGDGRDLDELVKEFNRARLEQG
                     SSFARARAEADVVVATRSGAS"
     gene            2097..2702
                     /gene="leuD"
                     /locus_tag="VGB93_10450"
     CDS             2097..2702
                     /gene="leuD"
                     /locus_tag="VGB93_10450"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0146544.1"
                     /translation="MDKFTSLTGVAAPLAIRNVDTDMIIPKQYLKTIKRTGLGTGLFS
                     EMRYKDDGSENPDFVLNKPAYRHAQILVAEDNFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFYNNCFKNGILPIVVKPDELAKLMDDAERGANATLSVDLESQEIRGPDGGVIHF
                     DIDPFRKHCLLNGLDDIGLSLQKAHHIDRFEAKAKGERPWH"
     gene            3004..>3873
                     /locus_tag="VGB93_10455"
     CDS             3004..>3873
                     /locus_tag="VGB93_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0146545.1"
                     /translation="MNEMMVRALRTIGFDVAFQRGSGQYLFDKGGTRYLDLLSGWGVF
                     GIGRNHPVVRQALETVLAADLPNLVQMDVTALSGLLAERLLQFVPFLDKVFFANSGTE
                     AVEAAIKFARGATGRPNIVYCSHAFHGLTYGALSLNGDATFRDGFGGFVPGCIEVPLN
                     DLAALEQALSRRDVAAFIVEPIQGKGVNMPDDGYLAGVRALCTKYGTLLVADEIQTGV
                     GRTGRFLAIEHWNVEPNMVLLAKALSGGHVPIGAVLMRRKIFESVFNRMDRMVVHGST
                     FAKNDLAMAAGLAT"
BASE COUNT          734 a         1166 c         1201 g          772 t
ORIGIN      
        1 cgtcacggcc tttgcaacgg gcgaccgtga acttctggcg cgtctgctcg acaaggatgt
       61 taatggcagt ttcgccagcg cgatcgatgc ccgcgcggcg cgcggcgaat cgctcatgac
      121 caaaatcgtt tccattgata agaccggcgt tttcgatgcc ggcgtacgcg acggcatgtt
      181 gcagatcacg ctgcgcttca tggcgagcct catcagcgcg acgcatgaca aggacggcaa
      241 actgatcgat ggcgatccgg aaaagccggt gaacatggtc gatctatgga cctttgctcg
      301 ccctgccgcg tcgcgcgatc ccaactggaa gctcgtcgcc acacagacag ggcattgagg
      361 caggctcccg aaaagcggta agctttccgg agagacatcc gacgatgtcc ataaatccgg
      421 cgctgacggc gcttgctatg actcttgcca cgggcgcgca tgagcgaaga gacacgcgag
      481 acattgcgcc cgctgtccca cgaggaagtt accctctggc tgacagtcac gcgccatgtc
      541 ctgcgccgtt ccgggataaa agcgccccta gcgccgccgc ctaaacctga aaagcccgtc
      601 aaaagcgcgc cgtcaccggg gcccgcgcag gtcaggcccg aacccccgcc gcgtccggct
      661 atgccgggcc tcgcgccgct ggagcgccgt gcccgtcaaa agctgtcgcg gggccatata
      721 ccggtcgatg ccgcgctcga tttgcatggc ctgcgccagc atgaggcgca tcctgccctg
      781 catagctttc taattcgcgc ccagcaccag ggggccaaga tcgtgcttgt cgtcaccggc
      841 aagggggagg gccgcaccgg tgatgtttac gaggaaggcg gcgtgctgcg ccgcaatgtg
      901 ccgatctggc tgcgggcgcc ggaatggcgc aatctcgtcg tcggcttcga ggaagcggcg
      961 cgccatcacg gcggcgccgg cgcgctctat atccgcattc gccgtaggtc gcggggggaa
     1021 gattgatacc gatcaattgg ccagatctcg taccgccgtc gcttgccgat ttcgagacgc
     1081 ttgccgacgc ggcttatgcg cgacttccgg atgatttccg cgcgctctgc caaggcatca
     1141 tcattcgggt tgatgatttt cccgatgacg aggtgctcga ccggatggcc tgcgaaaccg
     1201 aattcgacct gctgggcctt ttctccggtc gcggcctcgc ccaaggtggt gcccagtcct
     1261 ttaccgggca gatgccgaat atggtctggc tttaccggcg gccgatcctc gattattggg
     1321 ccgaaaacga ggacactctc ggcgaaatcg tcacccatgt cctggtgcat gagatcggcc
     1381 atcatttcgg tctctccgac gccgatatgg aagcgatcga ggcggcgggg gattagcccc
     1441 tatcctctgg agcaatgccg atgttcgcaa ccattgcctc cgccatctca gcctgcgtcg
     1501 ccgcctgcgg ccttttcttc gcctggcgac agtcgcgcga agcgaccttg cgccgcgccg
     1561 atgtgctgcg gtgggcggag gacgcgatcg accagttgca gacccttctg gtgatttgcg
     1621 tcctggatga gggcgaattg gagcccgcgt ttgaaaggga gaagctcgcc gcgatcatgt
     1681 tgaccacttc gacgcttgcc gagcgcggac gactcttctt caaaaacgag attcgcggcg
     1741 acggccacgg ccaggaaaag gaactggctt accaaggcta taggcccaaa atccttgacc
     1801 aactcgtagt cgcgcaccaa atcgctagac actggcctca ggcgaatgcc gacgatcgga
     1861 agatcatgct gatcctggcg caggacgctg cccggacttt catctcgctg gcgcagcagg
     1921 aggtcggccg cagcaaaacg gcaagcgccg tcaccaaacg ggcgggggat ggccgcgacc
     1981 ttgacgaatt ggtcaaggag ttcaatagag cacgcctaga acaaggttcg agctttgcgc
     2041 gcgcccgcgc cgaggccgac gtggtggtgg ccacccgatc tggagcatcc tgagcgatgg
     2101 ataaatttac gtccctgacg ggcgtcgcgg cgccgctggc gatccgcaat gtcgacaccg
     2161 acatgatcat cccgaagcaa tatctgaaga cgatcaagcg caccggcctc ggcacggggc
     2221 tgttctccga aatgcgttac aaggatgatg ggtcggaaaa ccccgatttc gtgctcaaca
     2281 agccggccta tcgtcacgcg cagattctgg tcgccgaaga caatttcggc tgcggctcgt
     2341 cgcgcgagca cgccccttgg gcactgctcg atttcggtat ccgctgcgtc atttcgacga
     2401 gctttgccga tatcttctat aacaattgct tcaagaacgg cattttgccg atcgtcgtga
     2461 agccggacga acttgccaag ctgatggacg acgcggagcg gggcgccaat gcgacgctct
     2521 cagtcgatct cgaaagtcag gaaatccgcg gcccggacgg cggcgtgatc catttcgaca
     2581 tcgatccgtt tcgcaagcat tgcctcttga atggtctcga tgacatcggc ctgagcctgc
     2641 agaaggcgca ccatatcgac cggttcgagg cgaaggcaaa aggcgagcgc ccttggcact
     2701 gacatgaatt caggcccgat atgtgaactc agtgacttct atgtcattga agtttactcg
     2761 catttctggt tttaaagagg tttcggtcga aagccggatg gtctagtgag agaccaccgg
     2821 atttgctact ctgccggtcg ccgaggcgtg aagccgcgat gccgccggcg ctggagaatt
     2881 tccccggtga atgatctgac aatgccacag gaagccgggt tcgatcggtc cccgcgtcca
     2941 ttcgatctcg ctgcattgct caaagatcgc gagcccgagc gataccaact gcatacgctg
     3001 tatatgaacg agatgatggt gcgggctttg cgcaccatcg gcttcgatgt cgccttccag
     3061 cgcggcagcg ggcaatatct gttcgataag ggcggcacgc gttatctcga tctcttgagc
     3121 ggttggggcg tgttcggcat cggccgcaat catcccgtcg tgcggcaggc cttggagacc
     3181 gtgctcgcgg ccgatctgcc gaatctcgtg caaatggatg tcacggcgct gtcgggtctg
     3241 ctcgccgaac gcctgctgca attcgtgccg tttctcgaca aggtgttttt cgctaattcc
     3301 ggtaccgagg cggtggaagc cgcgatcaag ttcgcccggg gcgcgactgg ccggccgaat
     3361 atcgtttatt gcagccacgc ctttcacggg ctgacctatg gggcgctctc gctgaacggc
     3421 gatgcgacgt ttcgcgatgg gttcggcggc tttgtgccgg gctgcatcga agtcccgtta
     3481 aatgacttgg ccgcgctgga gcaagcgtta tcgagacgcg acgttgctgc gttcatcgtc
     3541 gagccgatcc agggcaaggg cgtcaatatg cccgacgatg gttatctggc gggggtgcgg
     3601 gcgctctgca ccaaatatgg caccttgctc gtcgccgacg agattcagac gggtgtcggc
     3661 cgcaccgggc gtttcctcgc gatcgaacat tggaatgtcg agccgaatat ggtgctgctc
     3721 gccaaggcgc tgtccggcgg ccatgtgccg atcggcgcgg tgctcatgcg ccgcaagatt
     3781 ttcgagagcg tcttcaaccg gatggaccgc atggtcgtgc atggctcgac cttcgcgaaa
     3841 aacgatctgg cgatggcggc aggcctggcc acg
//
LOCUS       DASWOG010000506         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_213311,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000506 DASWOG010000000
VERSION     DASWOG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213311"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="cheB"
                     /locus_tag="VGB93_10460"
     CDS             complement(<1..789)
                     /gene="cheB"
                     /locus_tag="VGB93_10460"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY0146546.1"
                     /translation="MNKTRVLVVDDSRTMRHLISQILAQDPDIEVVGQAADPLEAREA
                     IKALEPHVITLDVEMPNMNGIKFLSKIMKLRPTPVIMISNLTARGTEATIKALEIGAF
                     DCIAKPAPGDRELFPDLAAKVKAAAAARLHLMKPPRQDAPTPAVPKQSIYESDGRIIA
                     IGASTGGVEALIAVLSKFPENCPPTVVTQHMPATFTKSFADRLDRLCAPRVSEASDGA
                     PLKQGQIYLAPGTTTHLEIVAGRGLSCRLSNAGPVNGHRPSVDVM"
     gene            complement(786..1685)
                     /locus_tag="VGB93_10465"
     CDS             complement(786..1685)
                     /locus_tag="VGB93_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HEY0146547.1"
                     /translation="MTSVPTKAIEAQRASAAISAEFVMTDADFRRIAEMLHADAGIHV
                     PNGKAALVYSRLAKRLRALGLESFRDYCELVSSHAGVSERQQMLAALTTNVTRFFREP
                     HHFEHLRKQVLPALIEKARHGGRVRFWSAACSNGAEPYSIALTILSMLPDAANYDIRV
                     LATDIDPNMVAEGTAGTYSDALMQPVPSDQRLRWFSSTRGAGGKVWTASDALRTLVAF
                     RELNLIGNWPMRGQFNAIFCRNVVIYFEADTQANLWSRFAPQLVPGGHLYIGHSERVS
                     GPAAARFESDGITTYRLRQEARK"
     gene            complement(1682..>2578)
                     /locus_tag="VGB93_10470"
     CDS             complement(1682..>2578)
                     /locus_tag="VGB93_10470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY0146548.1"
                     /translation="EVDKTVIERLSDPLTHMIRNAIDHGLEKPETRIAAGKPEEGTVR
                     LSALHRSGRIVIEISDDGAGINRPRVKQIAIDKGLIAADAMLSDDEIDNLIFLPGFST
                     ASEISDISGRGVGMDVVKRSIQALGGRISISSRPGRGSTFTMSLPLTLAVLDGMVVTV
                     ADQTLIVPLTAIVETLQPKPGEVHSMGGQARVISIREKFTPLIDISRELGYRDGASDA
                     FASVVILVETEGGGRSAFLVDAIQGQRQVVIKSLEANYRHVPGIAAATILGDGRVALI
                     LDVDAVIANTQENIGSGVSEAA"
BASE COUNT          492 a          784 c          801 g          501 t
ORIGIN      
        1 catgacatcg acagacggcc gatggccgtt gactggtccg gcgttgctca agcggcaact
       61 caagcctctg ccggcaacga tttcaagatg cgtcgttgtg ccgggcgcga gatagatctg
      121 gccctgcttc agcggcgcgc cgtcgctggc ctcgctcaca cgcggcgcac acaaccggtc
      181 aagccgatcg gcgaaactct tggtgaacgt cgccggcatg tgctgggtta cgaccgtcgg
      241 cgggcaattc tcgggaaatt tcgagaggac cgcgatcaag gcttcgacgc caccggtcga
      301 ggcgccgatc gcgataattc tgccgtcgga ttcatagatg ctctgctttg gcacagcagg
      361 tgtcggcgca tcctggcgcg gcggtttcat gagatgcagc cgcgcggcgg cggctgcttt
      421 cactttggcg gccagatcgg gaaataattc gcggtcgcca ggcgccggct tggcgatgca
      481 atcgaacgcg ccgatttcga gcgccttgat cgtcgcctcg gtgccgcgcg cggtgaggtt
      541 cgagatcatg atgaccggcg tcggccgcag cttcatgatt ttcgagagaa atttgatacc
      601 gttcatgttc ggcatttcga catcgagcgt tatgacgtgg ggctcaagcg ccttgatggc
      661 ctcgcgtgcc tcaagcggat cggcggcctg accgacgact tcgatatccg gatcctgcgc
      721 caaaatctgg ctgatcagat gccgcatcgt ccgcgaatca tcgacaacga gaaccctagt
      781 cttgttcact tgcgcgcctc ttgtctcaga cgatacgtgg tgatgccatc gctttcgaag
      841 cgtgccgcgg caggtcctga cacacgttcc gaatggccga tgtagaggtg gccgccgggc
      901 acgagctgcg gtgcgaagcg gctccacagg tttgcctgcg tatcagcctc gaaatagatc
      961 acgacgttgc ggcagaagat tgcgttgaac tggccgcgca tcggccaatt gccgataaga
     1021 ttgagttcgc ggaaagcgac gagcgtgcgc aaggcgtcgc tcgcggtcca gaccttgccg
     1081 ccggcgccgc gcgtggacga aaaccagcgc aggcgctgat cggacggcac gggctgcatc
     1141 aacgcgtcac tatatgtgcc ggcggttcct tcggcgacca tattggggtc gatgtcggtc
     1201 gccaaaaccc tgatgtcgta attggcggca tcggggagca tcgagagaat tgtcagcgca
     1261 atcgaatagg gctcggcgcc atttgaacac gccgcagacc agaaccgcac gcggccgcca
     1321 tggcgcgctt tttcgatcag ggccggcaga acctgcttgc gcaaatgctc gaaatggtgc
     1381 ggctcgcgga agaaacgcgt gacgtttgtc gtcagcgccg cgagcatttg ctggcgctcg
     1441 ctgacgcccg catgagacga gacaagctcg caatagtcgc gaaagctttc gagccccagc
     1501 gcgcgcaatc tttttgccag ccgcgaatag acgagcgcgg ccttgccgtt cggcacatga
     1561 ataccggcat cggcgtgcag catctccgcg atccggcgaa agtcggcatc cgtcatcaca
     1621 aattcggccg agatcgcagc ggaagcccgc tgcgcctcga tcgctttggt cgggacgctt
     1681 gtcatgccgc ttcgctaacc cccgagccga tattctcttg cgtattggcg atcacggcat
     1741 ccacgtcgag gatcagcgcg acgcggccgt cgcccaggat ggtcgcggcg gcaatgccgg
     1801 gcacatgacg ataatttgct tcgagactct tgatgacaac ctgccgctgc ccctggatgg
     1861 catcgacaag aaaagcgctg cggccgccgc cctcggtctc gaccagaatg accacgctcg
     1921 caaaagcgtc gctcgccccg tcgcgatagc cgagttcgcg gctgatatcg atcagcgggg
     1981 taaacttctc gcggatcgag atcacacggg cctgaccgcc catgctatgc acctcgccgg
     2041 gttttggctg caaggtttcg acaatggcgg tcagcggaac gataagggtc tgatcggcga
     2101 cagtcacgac catcccgtcg agcaccgcaa gagtcagcgg caagctcatc gtgaaggtcg
     2161 aaccgcggcc gggtcgcgaa gagatcgaaa tacggccacc gagcgcctgg atcgaacgtt
     2221 tcaccacgtc catgccgacg ccgcgcccgg agatatcgga gatttccgat gccgtcgaaa
     2281 atcctggcag gaaaatcaga ttgtcgatct cgtcgtcgct gagcattgcg tcggcggcga
     2341 ttagcccctt gtcgatcgcg atctgcttca cgcgtggacg gttgatccca gcgccgtcgt
     2401 cggaaatctc gatcacgatg cgcccggagc gatgcagcgc ggagagacgt accgtgcctt
     2461 cttccggctt gccagccgcg atgcgcgtct ccggcttttc caaaccgtgg tcgatggcat
     2521 tgcggatcat atgggtgagc ggatcgctca agcgttcgat caccgtcttg tcgacttc
//
LOCUS       DASWOG010000507         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_158403,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000507 DASWOG010000000
VERSION     DASWOG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158403"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VGB93_10475"
     CDS             complement(<1..1547)
                     /locus_tag="VGB93_10475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0146549.1"
                     /translation="MAKKKSDSNEIETAAASEPAKPYVVLARKYRPATFDELIGQSAM
                     VTTLRNAFASGRIAQGYMLTGVRGVGKTTTARILARALNYEAPGYDKPTIDMPGLGVH
                     CKDIMEGRHPDVLEMDAASNTGVDNIREIIESTQYTPLVARTKVFLIDEVHMLSKGAF
                     NALLKTLEEPPAHVRFIFATTEIRKVPVTVLSRCQRFDLRRVEQPVLAGHFQKIVGLE
                     GADADADALQLIARAAEGSVRDGLSILDQAIAMGEGHVTAAKVRDMLGLADRARIFDL
                     AESLFKGDVAAALQALSSLYRDGAEATEIVGDLAEAVHAIARVKAVGEEPSGEGLTAE
                     EKARLSSLASRLSMPYLSRAWQMLIKGFEETARAPNPRVAAEMVLIRLAYTSDLPTPD
                     ELIRTLGGGTAPARRPALAPSDGTPADKRAPLNSRAGAPVAESFEDEPNEGFEFDDGE
                     PIGDEDDAALPVSASKSDPRSFADVVALVSEKRNAKLTIHLEDHVSLVKFDASAGSID
                     LFLLPGAP"
BASE COUNT          334 a          550 c          493 g          307 t
ORIGIN      
        1 ggcgcacccg gcaaaagaaa gagatcgatc gatcccgccg acgcatcgaa cttcacgagg
       61 ctcacgtgat cctcgagatg aattgtgagc ttggcgttgc gcttctcgct gacgagtgcc
      121 accacgtcag caaacgagcg cgggtctgac ttcgacgcgg agacgggaag tgctgcatcg
      181 tcttcgtcgc cgatgggctc gccatcgtcg aactcgaaac cttcgtttgg ctcatcttcg
      241 aagctttccg caaccggcgc accggctcgc gaattgagcg gcgcacgctt atcggcagga
      301 gtaccatcgc tgggcgccaa agccggccgc cgcgcaggcg cggtgccacc acccaaagtc
      361 cgaatcagct catccggcgt cggcaaatcg gatgtgtagg cgagacggat cagaaccatc
      421 tcggcggcga ctcgcggatt aggcgcccgc gccgtctctt cgaagccttt aatcagcatc
      481 tgccaagcgc gagacagata cggcatcgac agccgcgatg cgagactgct caagcgcgcc
      541 ttctcttccg ccgtgagccc ttcgcctgac ggttcttcac caaccgcctt gacgcgggca
      601 atcgcgtgca cggcttcggc aaggtcgccg acgatctccg tcgcctctgc gccatcacga
      661 tacaatgacg agagcgcttg cagcgccgcc gcgacatcgc ctttgaaaag agattccgcg
      721 agatcgaaaa tccgcgcgcg atccgcaaga ccgagcatgt cgcggacttt cgcagccgtg
      781 acatggcctt cgcccatcgc gatcgcctga tcgagaatgg agagcccatc gcgaaccgaa
      841 ccttccgcag cgcgcgcgat caattgcaga gcgtcggcat cggcgtccgc accttcaagg
      901 ccgacgatct tctggaagtg cccggcaagc accggctgct cgacacggcg taaatcgaaa
      961 cgctggcagc gtgacagcac ggtaaccgga actttgcgga tttccgtcgt cgcgaaaatg
     1021 aagcgcacat gcgcaggcgg ctcttcgagc gtcttcagga gcgcgttgaa cgcgcccttc
     1081 gacagcatgt gaacctcgtc gatgaggaac actttggtgc gcgcaacgag cggcgtgtac
     1141 tgcgtgctct cgatgatctc gcgaatgttg tcgacgccgg tgttcgaggc ggcgtccatt
     1201 tcaagcacgt ccggatgacg gccctccatg atgtccttgc agtgaacgcc aagccccggc
     1261 atatcgatcg tcggcttatc gtagcccggc gcttcgtagt tcaacgcgcg cgcgagaata
     1321 cgcgccgtcg tcgtcttgcc gacgccgcgc acgccagtca gcatatagcc ctgcgcgata
     1381 cgccccgatg cgaacgcgtt gcgcagcgtc gtcaccatcg ccgactgacc aatcagctcg
     1441 tcaaacgtcg caggacgata tttccgcgcc agcacgacat acggcttcgc cggctcgcta
     1501 gccgcagcgg tttcgatctc gttcgagtct gatttcttct tcgccattga tccctgaaat
     1561 aacctttcgc cctaagggcg agggagcaaa gcgacccgcg gtaagccgcg cccccgcgga
     1621 gagcgccgga tcgcccttgc gatccgaatt gcgctcgtga gcgagtaaat aaggctggcg
     1681 aacg
//
LOCUS       DASWOG010000508         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_66855,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000508 DASWOG010000000
VERSION     DASWOG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66855"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VGB93_10480"
     CDS             complement(<1..659)
                     /locus_tag="VGB93_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY0146550.1"
                     /translation="MTLRRAMADTPRLHRRMSRRRRALVLLPALAALLCVLAIFAIGH
                     GPLPIAPRRVIEIIIGALRGVDQSSSREALVVLDIRLPRVLLAIMIGANLAVSGALMQ
                     SLFRNPLADPAIVGVSAGAALAAAAIIVLGDKYLTAGLPFVTLPAGAFAGALGTTLIL
                     YLIATRDRETSIATLLLAGIALGALAGAVTGLLTFVADDRQLRDLTFWSLGSLGGATW
                     D"
     gene            complement(656..1474)
                     /locus_tag="VGB93_10485"
     CDS             complement(656..1474)
                     /locus_tag="VGB93_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146551.1"
                     /translation="MLMPAAAAPNVPPPAQRIVAIGGAVTEILYALAQDGKIVGVDTT
                     SLYPPQALKSKPSVGYFRAFSPEGVLSLNPTLIIASDGAGPADAIELLRQSKVPVEKL
                     PDDFTAAGVVAKIETIGRLTHAESKAAELAKSVGGGFADVAALRGKADGKTHVLFIIS
                     MQDGRPLVAGNGTAADAMIKLAGGINVGADFAGYKQMNDESIVAAAPDVIVMMANGKQ
                     PDKNEIFGLPAFKTVPAAAHQRLVAMDGLYLLGFGPRTPKAAKDLFSAFYQGLK"
     gene            complement(1547..1714)
                     /locus_tag="VGB93_10490"
     CDS             complement(1547..1714)
                     /locus_tag="VGB93_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEY0146552.1"
                     /translation="MADEKDRTPSSEEPKRPPEIDVQELLGDAKEVTLLHGSERYRLR
                     ITANDKLILTK"
BASE COUNT          440 a          607 c          637 g          404 t
ORIGIN      
        1 ttgtcccatg tcgccccgcc aaggctgcca agcgaccaaa atgtgaggtc acgcaactga
       61 cggtcatccg caacaaatgt cagcagtccg gtcacggcgc ccgccaaagc gccaagcgcg
      121 atgccggcga ggagcagcgt agcgatggag gtttcccggt cgcgcgttgc gatgagatag
      181 agaatgagcg ttgtgccgag cgcgcccgca aacgcacctg cgggcagcgt tacgaatgga
      241 agaccggcgg tgagatattt gtcgccgagt acgatgatgg ccgctgcggc cagggccgcg
      301 ccagccgaga cgccgacgat ggcagggtcc gcgagaggat tgcgaaacag gctttgcatc
      361 aaggcgcctg agaccgcgag attggcgccg atcatgatcg ccagcaaaac gcgcggcagc
      421 cggatgtcga gtacgacgag cgcctcgcgc gaggaagatt ggtccacgcc acgcaaagcg
      481 ccgatgatga tctcgatgac gcgacgtggc gcgatcggca aaggcccgtg tccgatcgcg
      541 aagatggcca gcacgcacag gagcgcggcg agggcaggga gtagaacgag tgcccttcgg
      601 cgacggctca tgcgccgatg caggcgcggc gtgtccgcca tcgcccggcg cagcgtcatt
      661 tgaggccctg ataaaaagcg gaaaagagat ccttggcggc tttcggggtg cgcgggccga
      721 agccaagaag atacaggcca tccatcgcga caaggcgctg atgcgctgcg gctggcactg
      781 tcttgaacgc aggcaggccg aaaatctcgt tcttgtccgg ttgcttgcca ttggccatca
      841 tgacgatgac atcgggcgcg gccgcgacga tggattcgtc attcatctgc ttgtagccgg
      901 caaagtcggc gccgacgttg ataccgcctg caagcttgat catcgcatcg gcggccgttc
      961 cattgccagc gacgagcgga cgaccatcct gcattgagat gatgaagaga acatgcgtct
     1021 tgccatcggc tttgccgcgc agagctgcca catcggcaaa acccccgccg acgcttttgg
     1081 ccaattcggc ggctttcgat tcggcatggg tgaggcggcc gatggtttcg atcttcgcga
     1141 caacgccggc ggcggtgaaa tcgtccggca atttttcgac ggggaccttg gactgtcgca
     1201 acagttcgat ggcgtccgct ggacccgccc catcggacgc gatgatcaat gttggattga
     1261 gcgacagcac accttcgggc gagaacgcgc ggaaatagcc gacgctcggc tttgatttca
     1321 gcgcctgcgg cggatagaga cttgtcgtat cgacgccgac gattttcccg tcttgtgcga
     1381 gtgcgtagag gatctcggtg accgcgccgc cgatggcaac gattcgctgc gcgggcggag
     1441 ggacattcgg cgccgcggcg gcgggcatta acattgtcgc tatcgcgata aacgcgaaaa
     1501 gtcgccgttt gagagtcgat attttcaaca tagatgccgc gcgtacttat ttggtcagga
     1561 tgagtttgtc gttcgcagtg atgcgcagcc ggtagcgttc gctgccgtgg agcagagtca
     1621 cctctttcgc atcgccgagc agttcttgaa cgtcgatttc gggcggccgc ttcggctctt
     1681 cgctcgaagg cgttctgtcc ttttcatcag ccatcccata aaccccaaat gtcacacttc
     1741 cgtaggatca gacgtcgaaa atttagaatg gaactaattt atacttagaa tttagaatca
     1801 taataatata ataaaaacaa tacgttgctt gacccctcat catccattta ctacagacag
     1861 tcaagtcgcc gtcggggttc acggcgtcca atcagcaagc cagccagagc gcaaacgcgc
     1921 agagccagcc agccagcaaa atgcaacaag gcggggctct tcatgtcttc tatgattacg
     1981 cgcgcgctgc tcgccagcgt gtctttcttg tcgatctctt ctgcgtatgc gcagagcgca
     2041 tcgccgacgc aatctgcgtc ctccgcgccg caggccacca attcgccc
//
LOCUS       DASWOG010000509         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_341673,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000509 DASWOG010000000
VERSION     DASWOG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341673"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..710
                     /locus_tag="VGB93_10495"
     CDS             207..710
                     /locus_tag="VGB93_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0146553.1"
                     /translation="MPRLAHDPGYLQRMGFETYYFHGQLMVRQPPGEKNALGRIKFMF
                     PNQYSVYLHDTPEKRFFASTKRAFSHGCVRVDQPLDFAQTVLGPKWPQDRIESLIGGK
                     ERYVFLPKPLPIHIEYFTAYVSDDDRLIVRNDVYGYEHKIEEALGLVAAKPTNRNVAA
                     QFVPSRY"
     gene            1138..2703
                     /locus_tag="VGB93_10500"
     CDS             1138..2703
                     /locus_tag="VGB93_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY0146554.1"
                     /translation="MHSTAGKILSACLISSCLLLFAPSVTESAKANGDTRTIYLYHAH
                     TGESIDATYLVDGHYDPEVLKKLNWFLRDWRRNEQTNMDPRLFDAVWEAYRGAGATQR
                     ITVLCGYRSPETNAMLRRRSRAVAEHSQHILGKAMDTTMHGMSMEHVRESAARLQMGG
                     VGYYGDTNFVHIDVGGVRMWPRMTYAQLMRLFPDGKTVHIASDGRTLPGYDEARAELA
                     ANGTAVASLPPSQHSGIGAFFAWLFGKGNSGDDEDNAATEQQPTASASTQVAAAQAPA
                     PAEAMQADATGSIPDRSSDARAAEAAAAPDAVTASIDMPLPPQRPDDLVTAADVPLPP
                     ERPAFQLTALLSPAAPAKAAPVVVAEAETSAPDLPGIITQGPDDGRPRQGLSRMYGVP
                     ALVLAYAPVAQMEGLRRAAHHPEAMAPIPVNNVTIVPARLDGSNFGSLTSGTEVAELA
                     SATVFGPTAIGLRSAARVESAALFTQSPADYRERFQRAPATLRVDRFAMASVPALRQD
                     DGAVALDDSRSGD"
     gene            3051..>4526
                     /locus_tag="VGB93_10505"
     CDS             3051..>4526
                     /locus_tag="VGB93_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0146555.1"
                     /translation="MGPCFDEMTLPDGSTRAVYEKVARWLKEEPPEHLASRRTQAELL
                     FRRIGITFAVYGSKDAAERLIPFDIVPRVIGSAEWARLETGLFQRVNALNMFLADIYG
                     AQEILKSGRIPPDIILSNPFYLPMMRGLKVPHNVYVQIAGIDVVRVDANDFYVLEDNL
                     RTPSGVSYMLENREVMMRLAPDLFAEHRVAPVENYPDILLATLRSVAPESTQGEPSIA
                     LLTPGQFNSAYYEHSFLADRLGVELVEGRDLLVRDDIVYMRTTEGPKRVDVIYRRIDD
                     AFLDPLAFNSESVLGVPGLFSAYKAGNVTLANAVGTGIGDDKAIYSYVPDIIEFYLGE
                     KPILKNVPTWRCREPEALAYVLAHLPELVVKEVNGSGGYGMLIGPHATQAELALFAEK
                     LRIDPNNFVAQPTLALSTCPTALDSGVAPRHVDLRPFVLSGSDGVRVVPGGLTRVALK
                     EGSLVVNSSQGGGTKDTWVVSDAGLMDATGVIGQTQSQTSSF"
BASE COUNT          902 a         1365 c         1336 g          923 t
ORIGIN      
        1 gggctacatg gatgaaatag ataacgagaa acgttcggcc ctagaaacca ttgtggcctc
       61 aactaaccgc atgaatgagc taattagtac gctgcttaat ataactaggc tagaaagtgg
      121 tgggttagta atcgaaccac ggccagtaga tatagtccaa atagtcagtg aagtaatgga
      181 tgagatggct atactagcaa aaagtcatgc cgcgtctcgc gcacgatccg ggctaccttc
      241 agcgcatggg ctttgagacc tattatttcc acggtcaatt gatggtgcgc cagccgccgg
      301 gcgaaaagaa tgccttgggc cgcatcaagt tcatgttccc caaccaatat tcggtctatc
      361 tccacgatac gccggaaaag cgcttttttg catcaacgaa acgcgccttc agccacggtt
      421 gcgtccgcgt cgatcagccg ctcgactttg cgcaaacggt gcttggaccg aaatggccgc
      481 aggaccgcat cgaaagcttg atcggcggca aggagcgtta tgtgtttctg ccgaagccgc
      541 tgccgatcca tatcgaatat ttcaccgcct atgtgagcga tgacgaccgg ctgatcgtgc
      601 gcaacgacgt ctacggctat gagcataaga tcgaggaggc gctgggcctt gtcgcggcaa
      661 aaccgacgaa tcgcaatgtc gccgcgcaat tcgttccatc gcgatattga cggcgaggcg
      721 tcgaatcagc gattcttttt gaattgaaac gaaaagcggc gttcgtttcg gctcccgtcc
      781 gcgattccgg gcgtgaagag aaagctcgct cgccgacaca ggttcgccac atttgctggc
      841 ttacctgaac tggtcttcat cattcggcaa catcaagagt gtcagggatg tgaggcgacc
      901 gttgaacggg ggtagcgttt cgttccagaa gcgctcacgg ttctcggtaa atgcaggtat
      961 cggcggagac gcccgaaaaa gcagatcggc ttaaccgccg catccgagaa ccggggattt
     1021 gttttgccaa aaccgcgctt gagcacggat tctgtagtat gtgcgcggtg gcgccgggtc
     1081 gaggcagtag caaatttgct cagggtaaaa tcgagcgcga tcaaatccgc tcttaagatg
     1141 cattccacgg ccggcaaaat tctgtcggcc tgcctgatca gctcctgcct tctcttattt
     1201 gcaccttccg ttacagaatc ggcgaaagcc aacggtgata cgcgcacgat ctatctctat
     1261 catgcgcata cgggcgaatc gatcgacgcc acatatctcg tcgacggcca ttacgatccc
     1321 gaagttctca aaaagctgaa ctggttcctg cgcgactggc ggcgcaacga gcaaaccaat
     1381 atggacccgc gccttttcga cgcggtgtgg gaagcctatc gcggcgccgg cgcgacccag
     1441 cgtattaccg tgctttgcgg ctatcgttca ccggaaacaa acgccatgct gcggcggcgc
     1501 tcgcgcgcag tcgccgaaca ttcgcaacat atcctcggca aggccatgga cacgaccatg
     1561 catggcatgt cgatggagca cgtgcgcgaa tccgccgccc ggctgcaaat gggcggcgtc
     1621 ggctattatg gcgatacgaa tttcgttcat atcgatgtcg gcggcgtacg gatgtggccg
     1681 cgcatgacct atgcgcaact catgcggctg ttccccgacg ggaagaccgt gcatatcgct
     1741 tcggacgggc ggacgctgcc gggctatgac gaagcgcgcg cggaactcgc cgccaatggc
     1801 accgccgtcg caagcctgcc gccgtcgcag cattcaggca tcggagcttt cttcgcctgg
     1861 ctcttcggca agggcaacag cggcgacgac gaggataacg ctgcgaccga acagcaaccc
     1921 acggcgtcag cgtcgacgca ggttgcggcc gcacaagcgc ctgcgccggc ggaggcgatg
     1981 caggctgacg ccacgggcag cattcccgat cgttccagcg acgctcgcgc tgccgaggcc
     2041 gccgctgcgc cggatgccgt gaccgcctca atcgacatgc cgctgccacc gcaacgtccc
     2101 gacgacctcg tcaccgcggc tgacgtgcct ttgccgcccg agcggccggc gttccaactc
     2161 acggcgcttc tgtcaccggc agcgccggcg aaagcggcgc cggttgtcgt cgcagaagcc
     2221 gaaacctctg cacctgatct tcccggcatc attacccagg gcccggacga tggccgcccg
     2281 cggcaagggc tctcacggat gtatggcgtt cctgcgctgg ttctggccta tgcgccggtg
     2341 gcgcagatgg aaggattgcg ccgcgctgcg catcatccgg aggcaatggc gcccatcccg
     2401 gtgaacaatg ttacgatcgt cccagcgcgc cttgacggct cgaatttcgg cagccttacc
     2461 agcggcaccg aggtggcgga actcgccagc gcaacggttt tcggcccgac ggccatcggg
     2521 ctgcgcagcg cggcgcgggt cgaaagtgct gctttattta cccaatcgcc ggcggattac
     2581 cgcgagcgct ttcagcgcgc gcccgcgacg cttcgcgtgg atcgtttcgc catggcttcg
     2641 gttccggcgc tacgtcagga tgacggggct gtcgctcttg acgatagccg ttccggcgac
     2701 tgagccgatc tatcgaatcg ccaaaatctg aactgtgggc gcctgcgctg cgcccgcggg
     2761 cgtttcccgt agcatccagg ccatcgccag cgcgaaggcg gcgccgagcg agagggcgcg
     2821 aaacatttgc gccattgtta cgaccggacc gcagaagcgg cgactacgga ctttcgacat
     2881 accgaattgc tctgtttcac cggcgcgcgg cttatgccca gagtgttaac cttaattggt
     2941 tagcgatgct ttacctcgcg cttggccgcg gagtctccga ctgacgtcca gttgcatcga
     3001 ttttgcttaa tatgattcga tactaataat tagcggaccg ggttgcgcac atggggccgt
     3061 gttttgatga aatgacgctg ccggacggct caacgcgcgc cgtctatgaa aaagttgctc
     3121 gctggctcaa ggaagagccg ccagaacatc tcgcgtcgcg gcggacgcag gcggagcttc
     3181 tgttccgccg gatcggcatc acgttcgcgg tctatggcag caaggatgcg gccgaacggc
     3241 tgatcccctt cgatatcgtg ccgcgcgtga tcggcagtgc cgaatgggcg cggctggaga
     3301 ctggtctctt ccaacgcgtc aacgcgctca acatgtttct cgccgatatt tacggcgcac
     3361 aggaaatcct caaaagcggt cgcattccgc cggatatcat cctcagtaat ccgttctatc
     3421 tgccaatgat gcggggcctc aaagtcccgc acaatgtcta tgtgcagatc gccggcattg
     3481 atgtcgtccg cgtcgacgcg aatgatttct atgtgctgga agataatctg cgcacgccat
     3541 ccggcgtctc ttacatgctc gaaaatcgcg aagtcatgat gcggctggcg ccggaccttt
     3601 tcgccgagca tcgcgtcgcg ccggtcgaga attatcccga catcctgctc gcgacgctgc
     3661 gctccgttgc gccggagtca actcagggcg aaccgagcat cgctctgctg acaccgggcc
     3721 aattcaattc cgcctattac gaacattcct tcctcgccga caggcttggc gtcgaattgg
     3781 tcgaagggcg cgatctcctg gtccgggatg acatcgtcta tatgcgcacg accgaagggc
     3841 caaagcgcgt cgatgtcatt taccggcgca tcgacgatgc tttcctcgac ccgctcgcct
     3901 ttaattcgga gtcagtgctc ggcgtaccgg gtcttttttc tgcctataaa gcaggcaatg
     3961 tgacgctggc aaatgcggtc ggtacgggca tcggcgacga caaggcgatt tacagctatg
     4021 tgccggacat catcgaattc tacctcggcg aaaagccgat cctgaaaaac gtgccgacct
     4081 ggcgctgtcg tgagcccgag gcgctcgcct atgtgctcgc gcatctgccg gaactcgtgg
     4141 tgaaggaagt caacggctcc ggcggctacg gcatgctcat cggcccgcat gcaacgcagg
     4201 cggagctggc gctcttcgcc gaaaagctcc gcatcgatcc gaacaatttc gtcgcccagc
     4261 cgaccctcgc gctttcgact tgcccaacag cactcgatag cggcgttgcg ccgcggcatg
     4321 tcgatttacg cccctttgtc ctctcggggt cagatggggt gcgtgttgtg ccgggcggcc
     4381 tcacacgcgt ggcgttgaag gaaggctcgc ttgtcgtcaa ttcctcgcag ggcggcggga
     4441 ccaaggatac ctgggtggtc tccgatgcag gccttatgga cgccacgggt gttataggtc
     4501 agactcaaag tcagacgtct tcgttc
//
LOCUS       DASWOG010000510         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_196212,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000510 DASWOG010000000
VERSION     DASWOG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196212"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="VGB93_10510"
     CDS             <1..109
                     /locus_tag="VGB93_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY0146556.1"
                     /translation="YDRAAAPCPRPGCGGTIKRIVQSGRASFYCNTCQK"
     gene            complement(193..1359)
                     /gene="recF"
                     /locus_tag="VGB93_10515"
     CDS             complement(193..1359)
                     /gene="recF"
                     /locus_tag="VGB93_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY0146557.1"
                     /translation="MNDARAIVKELPARPAVTRLVLADFRSYPALDLDLVPGLVVLTG
                     ENGAGQTNLMEAMSRLSPGRGLRRAELGECALAGGTGTFAVSVEITTRDEDKLQLGTG
                     TEIQPDGGIVRRYRLNREPVTSIRSFADYIRIVWLTPAMDGLFSGSAGERRRFLDRLV
                     LALDGEHGGRVSALERALRNRNRLLENGSRDWQWLDAAEREVAELAVAVAAARVETVA
                     HLMQVILQDKNTDSPFPFAELTLKGDVEDLVATRPAIEAEDVYRDVLHQNRARDAAAG
                     RTLVGPHAADLLVVHGPKQADAARSSTGEQKALLVGIVLAHARLVAVLSGIHPIVLLD
                     EIAAHLDASRRAALYDRLVALGSQVFMTGADRAAFAGLEDRAQFLRVTPGHVEA"
     gene            1410..1889
                     /locus_tag="VGB93_10520"
     CDS             1410..1889
                     /locus_tag="VGB93_10520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0146558.1"
                     /translation="MGHKNSSEVQDYRLGPRGTDLAPLADLWVAAWLAVMPQIDFPAR
                     RAWLVTCVKDIEAHGGTTICAYDAESTLAGFSLLDLDRAYLEQIAIAPPLFGTGVGRL
                     LIGEAQRLCPDGLALDVNADNLRALRFYENAGFLRGEPGANPVSGLATWRMHWQGLT"
     gene            complement(1897..2283)
                     /locus_tag="VGB93_10525"
     CDS             complement(1897..2283)
                     /locus_tag="VGB93_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146559.1"
                     /translation="MKIFRRAAPILGLLSFGLSAAPLAAAPRTINDCEKIQAADAYNQ
                     CLASFGPVAHMHGMSSGPDAGGDEGGDAGGETGEALVRHHGHRAGGSHNGRVYMELDA
                     GANSAPVLTHRHWTHWHSHRGHHRHH"
     gene            complement(2280..4712)
                     /gene="pheT"
                     /locus_tag="VGB93_10530"
     CDS             complement(2280..4712)
                     /gene="pheT"
                     /locus_tag="VGB93_10530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0146560.1"
                     /translation="MKFTLSWLKQHLDTNADLATIVDTLTKVGLEVEHVHDPAAALRD
                     FVIAEVLEAHPHPNADRLRLCFVEIGSGKPVQVVCGAPNARAGMKTVFAPPGAYIPAK
                     GITLSKGVIRGVESAGMLCSGAELEVSTDQEGILDLPADAPVGTAYAKWAGLDDPLIE
                     INLTPNRPDAAGVLGIARDLDAAGIGQLQMPPVEPIAGSFPCPVKVSLDLAEADHHLA
                     PAFALRLVRGVKNGPSPDWLQKQLRAIGLRPINALVDITNFLTIDRARPLHVFDAAKV
                     AGDLHVRRAKTGESFLALDGKTYNLEENAIVIVDANGVESLAGIMGGEATGCTEATTD
                     VLIESALWDPVNIARTGRRLGINSDARYRFERGIDPASVLPGLARATQLVLEFCGGEA
                     SDIVLAGEIPDAHKRVTFPWSEVRRLAGVELSPSEMASILEHLGFRPSSLPGNSDLVI
                     IDVPSWRPDIEGKADIVEEILRIAGLDRITAQPLAREDEHISSAILTPLQKRVRSVKR
                     ALAANGLTEAINWSFIAHDAAQMFGGGDESLVLANPIASDLSDMRPSLLPGLIAASKR
                     NAARGFGDVALFEVGPIFRGTTPEDQKTMVAGLRRGRARVAGRGRHWADGASDADIFD
                     AKADALALLGALGVPQGGLQIGANAPRWFHPGRSGTFQFGPKNIVGTFGQLHPRVLEA
                     LDVDGTLVGFEIELDEIPLPKAKATKTKPKLELSDLMPVERDFAFLAERHIKAVDLVR
                     AIQSADRNLITDVTLFDIYEGAEIPPGKKSVAIAVTLQPRDKTLTDAEIDAVASRIIA
                     EVQNKTGATLRG"
     gene            complement(4907..>5446)
                     /locus_tag="VGB93_10535"
     CDS             complement(4907..>5446)
                     /locus_tag="VGB93_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY0146561.1"
                     /translation="PSAVFGPEDQCFNRFATLARLSPGMPLIGGGVTKLQPVYAGDVA
                     KAVAAGVKGSAAAGTIYELGGPEVRTLREIIEFILAETERKRFLMPLPFGPARGFGGI
                     SELVAKLTLGLMPEDFVLTTDQVELLQQDNVVSATAAAERRTLNGLGIAAESYEALVP
                     TYLTRYRKTGQFGTNDAFG"
BASE COUNT         1070 a         1709 c         1677 g          990 t
ORIGIN      
        1 ctatgatcgc gcagcagcgc cctgcccgcg ccccggttgc ggcggcacga tcaagcgcat
       61 cgttcaatcc ggccgcgcca gcttctattg caatacctgc cagaagtagc cgttattgcg
      121 aggagcaagg cgacgaagca atccagaaag cggtccatct actggattgc atcgcttcgc
      181 tcgaggtgac gctcaagcct ccacgtgccc cggtgttacc cgcaaaaatt gcgcgcgatc
      241 ttcaagcccg gcaaaagcgg cgcgatccgc gccggtcatg aacacttgcg atccaagtgc
      301 gacgagcctg tcataaagcg ccgcgcggcg cgacgcatcg agatgggcgg cgatttcatc
      361 aagcaggacg ataggatgga tgccgctgag cactgcaacg agccgcgcat gggcgagaac
      421 aatgccgaca agcagtgctt tctgctcacc ggtcgaggag cgtgccgcat ccgcctgttt
      481 cgggccgtgg acgacgagca gatcggccgc atgcgggcca acgagtgtgc gccccgcggc
      541 ggcgtcgcgc gcacgattct ggtgcagcac gtcgcggtaa acgtcttcgg cctcgatcgc
      601 cggccgtgtc gcgacaagat cctcgacatc gcctttcaac gtcaattcgg cgaagggaaa
      661 aggcgaatcc gtgttcttgt cctgcaggat cacctgcatg aggtgcgcaa ccgtctcgac
      721 ccgcgctgcc gcgaccgcaa cggcaagttc ggcgacctca cgctccgccg catccagcca
      781 ttgccagtcg cgcgatccat tttcgagcag gcgattgcga ttgcgtaatg cgcgttcgag
      841 cgcactgacg cggccgccgt gttcaccatc gagcgccaga acgaggcgat ccagaaatcg
      901 acgccgttcg ccggccgacc cggaaaacag accgtccatc gccggcgtta gccagacgat
      961 gcgaatataa tcggcaaaag atcggatcga cgtcaccggc tcgcggttca gccggtagcg
     1021 gcgcacaatt ccaccatcgg gctgaatctc ggttcccgtc ccgagttgaa gcttgtcctc
     1081 gtcgcgcgtg gtaatttcca ctgagacggc aaaggtgccg gtccccccgg cgagggcaca
     1141 ttcgccgagt tcagcccgac gcaatccgcg gcccggagac aggcgagaca tcgcctccat
     1201 caaattggtt tgcccggcac cattttcgcc tgtcagcacg acaaggcccg gcaccagatc
     1261 gagatcaagc gctggatagg agcgaaaatc ggcgagcacg agcctggtca cggcaggccg
     1321 ggcgggcagc tccttaacga ttgctcgggc gtcgttcatg tacattcatc taaagcagat
     1381 gcggagcctt ggaagcgcag gttgtgacaa tgggccacaa gaattcaagc gaggtgcagg
     1441 attaccggct cgggccgcgc ggcaccgacc tcgcgccgct cgccgacctc tgggtcgccg
     1501 cgtggctggc cgtcatgccg caaatcgatt ttcccgcgcg acgcgcatgg ctcgtgacct
     1561 gcgttaagga tatcgaggca cacggcggaa cgacaatctg cgcctatgac gcggaaagca
     1621 ccctcgccgg ttttagcctg ctcgatctcg accgcgccta tctcgaacag atcgccatcg
     1681 cgccgccttt attcggcacc ggtgtcggcc ggcttttgat cggcgaagcg cagcgtctgt
     1741 gcccagacgg tttggcgctc gacgtcaatg ccgacaatct gcgcgccttg cggttctacg
     1801 aaaatgccgg atttctgcgc ggcgagcctg gcgctaaccc ggtatccggg cttgctacct
     1861 ggcgcatgca ctggcagggc ctcacgtaag cgcgacctaa tggtgccgat gatgcccacg
     1921 atgagaatgc caatgagtcc agtgcctgtg cgtaagcacc ggggcggaat tggcgccggc
     1981 atcaagttcc atgtagacgc ggccattatg gctgccaccg gcccgatgcc catgatgccg
     2041 gacgagcgcc tcgccggttt cacctcccgc atcgccaccc tcgtcgccgc cagcatcagg
     2101 tcccgatgac atcccgtgca tatgggcgac cggtccgaaa gaggcgaggc attggttata
     2161 ggcgtcagcg gcctggattt tctcgcaatc gttgatggta cggggcgcag cggcgagcgg
     2221 cgcagcggac aaaccgaaac tcagcaagcc gagaatcggc gcagcgcgtc gaaaaatctt
     2281 catcctctga gcgtcgctcc ggttttattt tgaacttcag cgatgatcct gctggcgacg
     2341 gcgtcgatct cggcatccgt taaggtctta tcgcgcggct gcagagtgac cgcaatagcg
     2401 acggactttt tcccgggcgg aatctcggcg ccttcgtata tatcgaacaa ggtcacgtcg
     2461 gtaataagat tgcgatcggc cgattgtatg gcgcgtacga gatcaaccgc cttaatatga
     2521 cgctcggcta aaaaggcgaa atcccgctcg accggcatga gatccgacag ctcaagcttc
     2581 ggcttggttt tcgttgcctt ggccttgggc aaggggattt cgtcgagttc gatttcaaaa
     2641 ccgacaagag ttccatcgac atcgagcgcc tcaagcacgc gcggatgcaa ttggccgaaa
     2701 gtgccgacga tattttttgg cccgaattgg aatgtgcccg accggcccgg gtgaaaccag
     2761 cgcggcgcat tcgccccaat ttgcaaacca ccttgcggaa cgccaagcgc gccgagcaaa
     2821 gccagagcgt cggctttcgc gtcgaaaata tcggcatccg acgcgccatc ggcccaatgg
     2881 cgtccgcgcc ctgcaacgcg cgcgcgaccg cgccgcagac cggcgaccat ggtcttttga
     2941 tcctctggcg tcgtgcctct gaagatgggg ccgacttcga atagggcgac atcgccgaag
     3001 ccgcgcgccg cgttacgctt cgacgccgcg ataagacccg gcaaaagact tggccgcatg
     3061 tcggaaagat cggatgcgat cggattggcg agcacgagag attcgtcgcc accgccgaac
     3121 atttgcgccg cgtcatgcgc gatgaaggac cagttgatcg cttcagtcag accattggcg
     3181 gccagcgcgc gtttcacgct gcgcacgcgt ttttgtagcg gcgtcaagat cgccgacgaa
     3241 atgtgctcat cctcacgtgc caagggttgc gcagtaatcc tatcgaggcc ggcaatgcgc
     3301 aggatttcct cgacgatatc ggccttgcct tcaatgtccg gtcgccagga cggaacgtcg
     3361 atgatgacga ggtcgctgtt gccgggcagg ctcgaggggc gaaagccgag atgttcgaga
     3421 atcgacgcca tctccgacgg cgacagttca accccggcca gacgccgcac ttcgctccag
     3481 ggaaacgtca cccgtttgtg cgcgtctgga atctcccccg cgagaacgat gtcggacgct
     3541 tcgccaccgc aaaattcgag cacgagttgc gtcgcgcgtg cgagccccgg cagcacggac
     3601 gccggatcga taccgcgctc gaaacgatag cgcgcgtccg aattgatgcc gaggcggcgg
     3661 ccggtgcgag cgatgttgac ggggtcccac agcgccgatt cgatcagaac gtcggtcgtc
     3721 gcctcggtac agcccgtcgc ttcgccaccc atgatgccgg cgagcgattc gacgccattg
     3781 gcatccacga tgacaatggc attttcttcg agattgtagg ttttgccgtc gagcgcgagg
     3841 aagctctcgc ccgttttggc gcggcgcacg tgcaaatcgc ccgcgacttt cgccgcatcg
     3901 aagacatgca gcggtcgcgc cctgtcgatc gtcagaaaat tggtgatgtc gacaagcgcg
     3961 ttgatcggcc gaagcccaat ggcgcgcaat tgcttttgca gccaatcggg gctggggccg
     4021 ttcttgacgc cccgcacaag ccgcaacgcg aaggccggtg cgagatgatg atcggcttcg
     4081 gcaagatcga ggctgacctt cacagggcag ggaaagctgc cagcaatcgg ttcgacgggc
     4141 ggcatttgca attggccgat cccggctgca tcgagatcac gcgcgatgcc gagcacgcct
     4201 gcggcgtctg gtctgttggg cgtcagattg atctcgatca gcggatcatc aagccccgcc
     4261 catttggcgt aggcggtgcc gacgggcgcg tccgccggca gatcgagaat cccttcctga
     4321 tcggtcgaga cttcaagctc agctcccgaa cacagcatgc cggcggattc gacgccgcgg
     4381 atcacgcctt tgctgagtgt gatgcccttg gcgggaatat aggcgccggg cggtgcgaag
     4441 accgtcttca tgccggcacg cgcattgggc gcgccgcaga cgacctgcac cggcttgccg
     4501 ctgccaatct cgacgaagca caggcgcagc cgatccgcgt tcggatgcgg atgcgcttcc
     4561 agaacttcgg cgatgacgaa atccctcagt gccgcggctg gatcgtggac atgttcgacc
     4621 tcaaggccga ctttggtcaa agtgtcgacg atggtcgcga gatcggcgtt ggtatcgagg
     4681 tgctgcttga gccagcttaa ggtgaatttc atggagagtc cggttgcggg cacccgcggg
     4741 ccacgccttc ggcgcccacg gcgtcgccgg tatagagaca gaaggcggcg aattccagcc
     4801 ttcgagcacg acgcgacgct tcgcatctgc tccaaataaa aattcagggc gttttcaacc
     4861 gaatcggacg ccggttcgcg caaagaaaac gcgctgaaac aatgtcttat ccgaaggcat
     4921 cgttggtgcc gaactggccg gtcttgcgat agcgcgtgag ataggtcggc accagcgcct
     4981 cgtaggattc ggcggcgatg ccgaggccgt tcaaggtccg ccgctcggcc gcggccgttg
     5041 ccgagaccac attgtcctgc tgcagcaact cgacctgatc cgtcgtcagc acaaagtctt
     5101 cgggcatcag gcccaatgtc aatttcgcca ccaactcact tatgccgccg aagccgcgcg
     5161 ccggaccaaa aggcagcggc ataaggaaac gcttgcgctc ggtctcggcg aggatgaatt
     5221 cgatgatctc gcgcagcgtg cgcacttcgg ggccgccgag ttcgtaaatg gtccccgccg
     5281 ccgcactacc cttgacgccg gcggccaccg ccttggcgac atcgccggca tagaccggct
     5341 gcagtttggt gacgccgcca ccgatcagcg gcatccccgg cgacagccga gccagcgtgg
     5401 cgaagcggtt gaagcattga tcctccgggc cgaagacggc ggacgg
//
LOCUS       DASWOG010000511         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_213450,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000511 DASWOG010000000
VERSION     DASWOG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213450"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VGB93_10540"
     CDS             complement(<1..1671)
                     /locus_tag="VGB93_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0146562.1"
                     /translation="MPISQPNTAAVRRALFVSSSVIALAFSSSALAQLATGQAADQSQ
                     SLPEVLVTSPTTVPTPESDVASSISVITSQQMAAQQDRTVPDALSTVPGLNVVQTGGP
                     GGQTSVFLRGTNSNHVKVLIDGIDVSDPSNPNDSFDLGQLLTGDIERIEVLRGPQSGL
                     YGSDAIGGVINIVTKKGQGPLKVTGTIEGGSFGTANQTFDASGAHSIFNYAFNINHYL
                     AASTPVTPSNELPPGVQRNNDSYENWTFSTKLGADINEHLMLNYVGRYTDSTYFFTGD
                     GFDPSTGLEAPEGTQSLQIDHDFFTSGEAVTSFFEDRLKNYLGINYSDSHSFNASPGA
                     DNEINQGQRVKFDWRSVIVALPSETIVLGADHETESLFQNDPDVNASRNDAGGYAELQ
                     SNFYNRFFLVSNVRYDNYDTFGGHVTFRVAPAFIVPQTETKLKASYGTGFKAPTLSEL
                     FVNFPAFSFFANPDLLPEESTGYDAGFEQPLFHDRFSFGATYFHNNITNLIDSTDTTY
                     VNIGHAETQGVESFADWAVTDRLHLRTDYTYTRAVDESTGLELQRRPYD"
     regulatory      complement(1777..1967)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2079..2219
                     /locus_tag="VGB93_10545"
                     /pseudo
     CDS             2079..2219
                     /locus_tag="VGB93_10545"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
     gene            2436..2984
                     /locus_tag="VGB93_10550"
     CDS             2436..2984
                     /locus_tag="VGB93_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146563.1"
                     /translation="MSLSLQDHVAALAIGSAESSSRSIVVLAVPRVLTRQFPGGGLAS
                     LGEDTEAIQPDERKATLYDFGLGRAAGAFCMRTSSPALKASLDACLGAEWQQVLSLKG
                     ANILAESPVRVVRNPIGRIEVYSGIPQSGGVSPTGPHTHFLPAQIAAGGDLPATMQIP
                     RAYVPCVIYYPPLSDARSEHDH"
     gene            complement(3010..>3189)
                     /locus_tag="VGB93_10555"
     CDS             complement(3010..>3189)
                     /locus_tag="VGB93_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146564.1"
                     /translation="TGYNNRSEEARDEFIVLRKPFSATDLARVIARIQSEASPQGPTN
                     VIRLGQPRKSAKPES"
BASE COUNT          630 a          898 c          963 g          698 t
ORIGIN      
        1 gtcatatggt ctgcgctgca attctagacc ggtcgattca tccaccgcgc gcgtgtaggt
       61 gtaatccgtt cgcagatgca gccggtcggt gaccgcccag tcggcaaagc tttcgacacc
      121 ctgcgtttcg gcatggccga tatttacgta ggtcgtgtcg gtggaatcga tcagattcgt
      181 gatgttgttg tggaaataag tcgccccgaa gctgaacctg tcgtggaata aaggctgctc
      241 gaaaccggcg tcgtagccgg tcgattcctc cggcagaagg tctggatttg cgaagaatga
      301 gaacgccggg aaattcacga agagttcact cagcgtcggt gccttgaagc cggtgccgta
      361 gctcgccttg agcttcgtct ctgtctgcgg gacgatgaaa gctggcgcaa cgcgaaaggt
      421 cacgtggccg ccgaacgtgt cgtaattgtc gtagcgcacg ttcgagacga ggaagaagcg
      481 attatagaaa tttgactgca attccgcata gccgccggca tcgtttcggc ttgcgttgac
      541 gtccggatcg ttctggaaga gcgactccgt ctcgtggtct gcaccgagta cgatcgtttc
      601 gctcggcagc gccacgatca cgctgcgcca atcgaatttg acgcgctggc cctggttgat
      661 ttcgttgtcg gcgccggggc tcgcattgaa actatgagag tcggaataat tgatgccgag
      721 ataattcttc agccggtctt cgaagaagga cgttaccgct tcgccgctgg taaaaaagtc
      781 gtggtcaatt tgcaggcttt gcgttccctc gggcgcttct aggccggttg acggatcaaa
      841 cccgtcgccg gtgaagaaat acgtggaatc agtgtaacga ccgacatagt tcagcatcag
      901 gtgctcattg atgtcggcgc caagcttggt cgaaaaggtc caattctcgt aggaatcgtt
      961 gttgcgttgt acgcccggtg gaagctcatt cgacggcgtc actggtgtgc tggccgcgag
     1021 ataatggttg atgttgaacg cataattgaa gatcgagtgc gcgccgctgg cgtcgaatgt
     1081 ctggttggcc gttccgaatg agccgccctc gatcgttccc gtcaccttga gcgggccttg
     1141 gcctttcttg gtgacgatat tgatgacgcc gccgatcgca tccgagccgt aaagaccgct
     1201 ctgcggcccg cgcaaaacct caatgcgctc gatatcgcca gtcaacaatt ggcctaagtc
     1261 gaaagaatca ttgggattgc tcggatcgct gacatcaatg ccgtcgatca aaaccttgac
     1321 gtgattcgaa tttgttccac gaagaaaaac ggaagtctgg ccgcccggcc ccccggtctg
     1381 cacgacattg aggccgggga cagtgctcag cgcgtcaggg acggtgcggt cttgctgcgc
     1441 cgccatctgc tggctggtga tgacgctgat gctacttgcg acatcgctct caggcgtcgg
     1501 caccgtcgtc ggcgacgtca cgaggacttc cggaagcgat tgcgactgat cggcggcttg
     1561 gccggtcgca agctgcgcca aggcagagga cgaaaacgct agggcgatga cgcttgacga
     1621 aacaaaaagc gcgcggcgca ccgccgcggt attgggttgg gagataggca taaggcctcg
     1681 cagcaacgtg acacgtcact gcgagccaaa gcgatcaatg cgagcgatct aaacgcgcat
     1741 tcatccccca tcaggacacc ccgcccaatg tgttcgcgcg tgaccacggc ggatggcagg
     1801 tctcctggct cacgggtctt tgctttgcgc cgccttcccg gttgcccagt ggcttaatgg
     1861 cgttcagctc gccgcttaca gttgcggggg cagccgcggc atggatctga cgatcgcacc
     1921 gcgttccctt ttgatccccg aagggaacca tcacactcat gatgaagctg aagcccgaat
     1981 gctgtcaagt tcgatttgca caagttcgca gcttgcgaat gaatgcgtcc ccggcgtaaa
     2041 gctccgcgcc aattttttca gatcaagcgg tgtccgagat gattccaagt ccctgtatcg
     2101 gcgtatgccg catccgcccg tcttctgatt tctgtattgg ttgcgcgcgc agccgcgatg
     2161 aaatcgcaag ctggccaatc ctgaccgacg accaaaagag cgccgtttgg gcggcgctgc
     2221 cggatcgtcg tgcgcagatg gaacttagca ttcatcacct cggctggagc accgacgatt
     2281 tacgagcctt cctaagggac tcacttcgcc ccggcggcgg aacctgggtt tcgggtgtga
     2341 acggtgcaat cgcagaattt tgcatcggcg cagacgatga tttgcgtttg gaactgagcg
     2401 atagaacgat cagtgcgcgc acgccaagcg gtgcgatgtc tctttccttg caggatcacg
     2461 tcgcggcgct ggcaattgga tcggcagaat cctcaagccg cagtatcgtc gtccttgccg
     2521 tgccgcgcgt tctcacacgg caatttcccg gcggcggttt agcgagcctc ggagaagaca
     2581 ctgaagcgat ccagccagat gaacgtaagg cgacgctcta tgatttcggt ctcgggcgcg
     2641 cagcgggtgc gttctgcatg cggacgagtt caccggcgct caaggcgagt ctcgatgctt
     2701 gtctgggcgc ggaatggcaa caggtgttga gcttgaaggg cgcaaatatt ttggccgagt
     2761 cgccggtccg cgtcgtacgc aatccgatcg ggcggatcga ggtatatagc ggaattccgc
     2821 agtccggcgg cgtctcacct acaggtccgc acacgcattt cctgcccgcg caaattgcgg
     2881 ccggcgggga tttgcctgcc acgatgcaaa ttccccgcgc ctacgtgccc tgcgtcatat
     2941 attatccgcc gctctccgac gcgcggtcgg agcacgacca ctagcgcgtt tcgacgctga
     3001 tcctcgactt caactttcag gcttcgcgct tttacgcggc tgacccaaac ggataacgtt
     3061 cgtcgggcct tgcggtgagg cctcggactg gatacgggcg atgacgcgcg cgaggtcggt
     3121 ggcactgaag ggcttgcgca atacaataaa ctcatcgcgc gcttcctctg agcgattgtt
     3181 gtagccggt
//
LOCUS       DASWOG010000512         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_158547,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000512 DASWOG010000000
VERSION     DASWOG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158547"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="hisG"
                     /locus_tag="VGB93_10560"
     CDS             complement(<1..513)
                     /gene="hisG"
                     /locus_tag="VGB93_10560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0146565.1"
                     /translation="MSDVTNPPLVLAVPSKGRLQENAAAFFARAGLQFVQGRGARDYR
                     AKIAGLDNVEVAFVSTSEIVTHLAAGTAHLGVTGEDLVRESIPDADSKCELLAPLGFG
                     LANVIVAVPQAWIDVRTMADLEDVTVAVRARRGERMRVATKYDNLARHFFAARHVTDY
                     RIVESLGATEG"
     gene            complement(510..1667)
                     /locus_tag="VGB93_10565"
     CDS             complement(510..1667)
                     /locus_tag="VGB93_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0146566.1"
                     /translation="MSVSAEPSSAAARRAASAALVAHLEASGYPRYEPPVLQRAELFF
                     NSGEEIRSRLYLTSDLSGAEYCLRPEYTIPVCAHYLASEAVGRATTYSYCGPVFRYRP
                     DGLNEFLQAGLENFGHPDREAADAEILGVTLDAVAALGQGDLNVRFGDNGLVRDLVAA
                     LDLSPQWQRRILRGHAQDKSLDTILARPGNGKGADHTGVLAALEGVDRHGARALVEDL
                     LSIAGISSVGGRSVTEIADRFLEQAALKTGEEFPVEHRAILKEFLAIETDPDQAAAKL
                     RQLASTTGLDLNAALDRFDARLGFMAARGLDVADFTFATRFARDLEYYTGFVFEAFDR
                     SRPHEKPLVGGGRYDGLLKSLGCQGDVGAVGAAIWIERLQLPQRGQTDWWR"
     gene            complement(1756..>2110)
                     /locus_tag="VGB93_10570"
     CDS             complement(1756..>2110)
                     /locus_tag="VGB93_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146567.1"
                     /translation="GLVFPDFEACRGLGIGVATTPAEIAAHPAVRAKFQALLDGFAEA
                     ARSSSRRIARLLLLATPPLAEAGEWTEKGGINQRAVLANRAVMVTRLLSATPPAEVIA
                     AANSAISQQRHEIAK"
BASE COUNT          412 a          644 c          679 g          375 t
ORIGIN      
        1 gccttcggtc gcgccgaggc tttcgacaat tcgatagtcg gtgacgtggc gcgcggcgaa
       61 aaaatgccgc gcgagattgt cgtatttggt cgcgacacgc atccgctcgc cgcgtcgggc
      121 gcgaacagcc accgtaacgt cctcgagatc ggccatggtt cgtacatcga tccacgcctg
      181 cggcacggca acgatgacat tggcaaggcc gaagccgagc ggcgcgagaa gttcgcattt
      241 cgaatcggcg tcgggaatgc tttcacgcac cagatcctcg ccggtgacgc cgagatgcgc
      301 cgtaccagca gcgagatgcg tgacgatttc cgatgtcgag acgaaggcaa cctcgacatt
      361 gtcaaggccg gcgattttgg cccggtaatc gcgggcgcca cggccctgca cgaattgcag
      421 tccggcgcgc gcaaagaacg cggccgcatt ctcctgcaaa cggcccttgg aggggacagc
      481 gagaacgagc ggggggttcg tcacgtcgct catcgccacc aatccgtctg gccgcgctgc
      541 ggcaattgca aacgttcgat ccagatcgcc gcgccgacgg cgccgacatc gccctgacag
      601 ccgagcgatt ttaaaaggcc gtcgtagcgg ccaccaccga cgagcggctt ttcatgcggc
      661 cggctgcggt cgaaggcctc gaagacgaag ccggtataat attcgaggtc gcgggcgaac
      721 cgtgtcgcga aagtgaaatc ggcgacatcg agtccgcgcg cggccatgaa accgagacgc
      781 gcgtcgaaac ggtccaacgc cgcgtttaga tcgaggcccg tcgtcgaagc caactggcgc
      841 agcttggcgg cggcttgatc gggatcggtc tcaatggcga gaaattcttt caggatcgcg
      901 cggtgctcga cggggaattc ctcgccggtt ttgagtgccg cttgttcgag aaagcgatca
      961 gcgatctccg tcacgctgcg gccgccgacg ctcgaaatgc cagcgatcga gagcagatcc
     1021 tcgacaagag cacgcgcacc gtgccggtca acgccctcaa gcgctgcgag tacgccggta
     1081 tgatcggcgc ccttcccatt accgggacga gcgaggattg tatccagcga tttgtcttgc
     1141 gcgtggccgc gcagaatgcg gcgctgccat tgcggcgaca agtcgagcgc ggcgacgaga
     1201 tcgcggacca gtccattgtc gccgaaacgt acattgagat cgccttggcc gagcgcggcg
     1261 acggcatcga gtgtcacgcc caagatctcg gcgtcggcgg cctcgcggtc ggggtgtccg
     1321 aaattctcaa gccccgcctg cagaaattcg tttagaccat ccggccgata gcggaacacc
     1381 ggaccacaat agctgtaggt cgtcgcgcgc ccgaccgcct cggaagcgag ataatgcgcg
     1441 cagaccggga tcgtatattc gggccgcagg caatattcgg cgcccgagag gtcgctcgtc
     1501 agatagagcc ggctgcggat ctcttcgccc gaattgaaga agagttcagc gcgttgcaga
     1561 accggcggct catagcgcgg atagccggag gcttcgagat gcgcgacaag cgccgcggaa
     1621 gcggcacgcc tggcggctgc tgaggagggt tcggcgctca cggacatgtc tcgaatttgt
     1681 gcttctcttt agcaagtgcg cggcgcgggc gcgatgcctt tcgatctgca aggtgaatgg
     1741 caagaaaaca gcggtttatt tagcaatttc atgtctttgc tgtgaaatgg ctgagtttgc
     1801 cgccgcaata acctctgccg gcggtgtggc agacagaagg cgagttacca taaccgcccg
     1861 gttggcgagc acggctcttt ggttgatccc gcccttctcg gtccattcgc cggcttctgc
     1921 caaaggcggc gtcgcgagca gcaggaggcg cgcgatccgt cgtgatgagc ttctggcagc
     1981 ttcggcgaaa ccatccagca gagcctgaaa tttggcccgg accgccgggt gtgcagcgat
     2041 ctccgctggc gttgtcgcca cgcctatgcc gagacccctg catgcctcga aatccgggaa
     2101 cacgagcccc
//
LOCUS       DASWOG010000513         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_158573,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000513 DASWOG010000000
VERSION     DASWOG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158573"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..555)
                     /locus_tag="VGB93_10575"
     CDS             complement(268..555)
                     /locus_tag="VGB93_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146568.1"
                     /translation="MNLRSFLRDERASSFESLALALSVIAVLCVAAANFLHYAARKDG
                     ELAQLIRASHQEVARIFGNDAPLRGDIDYTSTGSIGALHNPPALNPCTEQK"
     gene            complement(719..>2235)
                     /locus_tag="VGB93_10580"
     CDS             complement(719..>2235)
                     /locus_tag="VGB93_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0146569.1"
                     /translation="VFKRGEPKSKLEQVGKIANRRGTQVRFKVDEQIFGKGAKFKPSR
                     LFRMARAKAYLFGGVEIRWHCAPQLIEAGSDIPAEAVFHFPGGLKDYLAQDIKDKETV
                     ADQSFTGKVEKPGGHGSLEWAVTWLAEDDGFVHSYCNTIPTPDGGTHEAGLRGALLRG
                     LKDHSERIGQAKRAAVLTSDDVMACCAALVSVFLREPEFQGQNKGRLMTAEATRIVET
                     TLRDAFDHWLAASPSAATKLLDFTIERAEERLRRRAEKDVARKSASRKLRLPGKLADC
                     SNNAAQGSELFIVEGDSAGGSAKQARDRINQAVLPLRGKILNVANATKDKVTANQQLA
                     DLIQALGCGTGAHYKDDDLRYEKIIIMTDADVDGAHIASLLITFFFRQMPKLIEHGHL
                     LLSVPPLYRLTQGSNHVYARDDAHREELMKTVLTGKAKVEVSRFKGLGEMPAAQLKET
                     TMDPRSRTLLRVVVVPADQEETADSVERLMGNKPEARFAFIQERAAFANDLVVV"
BASE COUNT          403 a          659 c          684 g          489 t
ORIGIN      
        1 cttgtcttcc cggctatatc ccttcgtcgt tccctcaatg ctaacttccg ggggatggaa
       61 ataggcccag cgagcgcccg acttactgca gaacgtgcag ttgcactcgt tgatgaagtc
      121 cggtcgcttc gggatttgga cgcggacttg gccacagagg cagaaaatct tcatcattcc
      181 cgcgttgtat caggagagcc gttcgcctaa tagcggctcg tcaatctcga ttatgtccgg
      241 tttctggctt ctcggtcttg agcagggtta tttctgctcg gtgcagggat tgagtgccgg
      301 cggattgtgc aaagcgccaa tgctgccggt ggacgtataa tcgatgtcgc cgcgcagcgg
      361 cgcgtcgttg ccaaagatgc gcgccacctc ctgatggctg gcacgtataa gctgggcgag
      421 ttcgccgtcc ttgcgcgcgg catagtgcag aaagttggcc gcggcgacgc aaagcacggc
      481 gatgaccgac aacgccaaag ccaagctttc aaagctgctc gcgcgctcat cgcgcaagaa
      541 agaacgcaaa ttcatggcct ctgaacctct aaccgccgca tactcaagat gcgggggcag
      601 ggttaagaga gcgttgacga attgcgttaa aagcgtggtt ttggcttaac gttccgatgc
      661 ggttgaaaag tgcatcggta ccgcccgaaa accgcttcac acttttcggt ccaatgctct
      721 atacgaccac gagatcgttg gcgaaagcgg cccgctcctg aatgaacgca aaacgcgctt
      781 ccggcttgtt gcccatcagg cgttcgacgg aatccgccgt ctcttcctga tcggcaggga
      841 cgacgacaac acgcagcagc gtgcggctcc tcggatccat tgtcgtctct ttcaattgcg
      901 ccgctggcat ttcgccgagg cccttgaagc ggctgacctc taccttggct ttgccggtca
      961 gcacggtttt catcagttct tcgcgatggg catcgtcacg cgcgtacaca tggttcgaac
     1021 cctgcgtcag ccggtaaagc ggcggcacac tgagaaggag gtgaccgtgc tcaatgagct
     1081 tcggcatctg tcgaaagaag aacgtgatga gcagtgaagc gatatgggcg ccgtcgacgt
     1141 cggcatccgt catgatgatg atcttctcgt agcgaaggtc gtcgtccttg taatgggcgc
     1201 cggtgccaca gccgagcgct tggatcaggt cggccaattg ctggttggcg gtgactttgt
     1261 ccttggtggc attcgcgacg ttgagaatct tgccccgcag cggcagcacc gcctggttga
     1321 tgcggtcgcg cgcctgcttt gcggaaccgc cggccgagtc gccctcgaca ataaataatt
     1381 cagatccttg tgctgcgttg ttcgagcaat ctgcgagctt gcccggcagc cgcagtttgc
     1441 gactggcgct tttgcgggcg acgtccttct cggccctgcg ccgcagccgc tcttcggcgc
     1501 gctcgatggt gaaatcgagc agtttggtcg cggccgaagg ggacgcggcg agccagtggt
     1561 caaaggcgtc gcgcagtgtc gtttcgacga tgcgggtcgc ttcggcggtc atcagccgac
     1621 ccttgttctg cccctggaat tccggttcgc gcagaaagac ggagaccagc gcggcgcaac
     1681 aggccatcac gtcgtcgctg gtgagcacgg cggcgcgttt tgcctggccg atgcgctcgg
     1741 aatggtcctt caggccacgc agcagtgcgc ctcgcaggcc ggcctcatgc gtgccgccat
     1801 caggtgtcgg gatcgtgttg caataggaat gaacgaagcc gtcgtcttcc gcgagccacg
     1861 tcaccgccca ttcaagcgag ccgtggccgc cgggcttttc gaccttgccg gtaaagctct
     1921 ggtcggcgac cgtttccttg tccttgatgt cctgagcgag atagtccttg agaccgcccg
     1981 ggaagtggaa caccgcctct gccgggatgt cgctgcctgc ctcaatcaat tgcggggcgc
     2041 agtgccagcg gatttcgact ccgccgaaga gataggcttt ggcccgcgcc atacggaaga
     2101 gtcggctggg cttgaatttg gcccccttgc cgaaaatctg ctcgtccacc ttgaagcgca
     2161 cctgggtgcc acggcggttg gcgatcttgc caacttgctc aagcttgctt ttgggctcgc
     2221 cgcgcttgaa gacct
//
LOCUS       DASWOG010000514         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_324358,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000514 DASWOG010000000
VERSION     DASWOG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324358"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGB93_10585"
     CDS             <1..235
                     /locus_tag="VGB93_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0146570.1"
                     /translation="VCIKVIAENLTTQESRHAISCYFTMVARGTDGQAREVPHLILET
                     ELDRRLFEAGKMRKEMREEIIERNRKLHVGMPD"
     gene            complement(257..1435)
                     /locus_tag="VGB93_10590"
     CDS             complement(257..1435)
                     /locus_tag="VGB93_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0146571.1"
                     /translation="MDAKAPFNAKQGNHFTGPSKSGGWRPDRCDQIALIFQGGGALGA
                     YQAGVYEALHEANIEPDWVTGVSIGAINAAIIAGNPRSERLEKLRAFWQRITDRHVWA
                     YTPDGDIYRRARNAASAMMTMTLGQPGFFKPHDVSPWLNAAGAKAATSFYDSAPLAAT
                     LSEFVDFSLINDGNTRFAVGAVNVLNGNFIYFDNRNEEIAAEHILASGALPPALPMVK
                     IGTDYYWDGGIVSNTPLQHLLDQDDLLNTLVFQVDLFSAQGLLPRDMADVLGRQKDIM
                     YSSRTRYNTDMYQRLRTWKNRAYEALAKLPDNLLTDNDRKLRDELADMPNIAILHLIY
                     QQKAYEGDAKDYDFSGTSMREHWQSGYEDTKRTLMHHVWFDMPENGIVVHDVHRQRDR
                     "
     gene            1583..2329
                     /locus_tag="VGB93_10595"
     CDS             1583..2329
                     /locus_tag="VGB93_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEY0146572.1"
                     /translation="MRAEDILHLPSMPLASPSYPKGPYRFVDREYMVITYETDPEIIR
                     HQLPEPLEPLDQPLVSYEWINMPDSSGFGSYTESGLVIPCKFQGEDVNFVSQMYLDDD
                     PPIAGGREIWGFPKKYAHPKLKVFKDTLTGTLHYGEQLVAMGTMGYKHEAMAGNGVGT
                     TAILSKTQINLKIIPGVDGGHGICQLVAYNLTEITVKGSWTGPARLHLVPHVNAPVGD
                     FPVRHIVGARHFIADLSLPYGRVVHDYLKP"
     gene            2482..3540
                     /gene="gmd"
                     /locus_tag="VGB93_10600"
     CDS             2482..3540
                     /gene="gmd"
                     /locus_tag="VGB93_10600"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0146573.1"
                     /translation="MSDSKVALITGVTGQDGALLAELLLNKGYIVHGIKRRSSLINTD
                     RVDHLYQDPHEADVRFSMHYGDLTDATNLIRIVQEVQPDEIYNLAAQSHVQVSFETPE
                     YTANSDALGTLRLLEAIRILKLVDKTRFYQASTSELYGKVQAVPQSETTPFYPRSPYA
                     AAKLYAYWITVNYREAYGIHASNGILFNHEGPTRAETFVTRKITRAVAAIQLGLQNKL
                     YLGNLDAKRDWGYAGDYVEGMWRILQQPVADDYVLATGETHAVREFVERAFAEIGIEI
                     AWRGAGVEEQGFDRKSGRVLVEIDPEYFRPTEVDLLLGDPSKARDKLGWVHKTTFPEL
                     VKEMVASDLVLMKKRGHI"
BASE COUNT          751 a         1079 c         1071 g          735 t
ORIGIN      
        1 ggtctgtatc aaggtcatcg ctgaaaatct gacaacgcag gaatcgcggc acgcgatctc
       61 gtgctatttc accatggttg ccaggggcac cgatgggcag gcccgcgaag tgccacatct
      121 cattcttgag acggaactcg atcgccgtct cttcgaggcg ggcaagatgc gcaaggaaat
      181 gcgcgaagaa atcatcgagc ggaacagaaa gctccacgtc ggcatgccgg attagtcgca
      241 agagcttacg cgcggctcag cgatcccgct gccgatggac gtcatgcacg acgatgccgt
      301 tctccggcat gtcaaaccag acgtgatgca tcagcgtgcg cttggtatct tcgtagccgc
      361 tttgccaatg ttcgcgcatc gacgtcccgg agaagtcgta atccttggca tcgccttcgt
      421 aggctttctg ctgatagatc agatgcagga tcgcgatatt cggcatatcc gccaattcgt
      481 cgcggagctt gcggtcgttg tccgtcagca aattgtccgg cagtttcgcc agcgcctcat
      541 aggcgcggtt tttccacgtg cgcagccgct ggtacatgtc ggtgttgtag cgggtgcgcg
      601 aggaatacat gatgtccttt tggcgcccga ggacgtcggc catatcccgc ggcaagagac
      661 cctgcgccga gaaaagatcg acctggaaga cgagtgtgtt gagcagatcg tcctgatcca
      721 gcaagtgctg cagcggcgtg ttcgagacga tgccgccatc ccaataataa tcggtgccga
      781 tcttgaccat cgggagcgcc ggcggcagtg cgccagaggc caagatgtgt tccgccgcga
      841 tctcctcatt gcgattgtcg aaatagatga aattgccgtt caggacgttc accgcaccga
      901 cggcaaagcg cgtgttgccg tcgttgatga gcgagaaatc gacaaattcg cttaacgtgg
      961 cggcgagtgg cgcggaatcg tagaagctcg ttgccgcctt ggcacccgcg gcgttgagcc
     1021 aagggctgac atcgtgcggc ttgaaaaagc ccggctggcc gagcgtcatg gtcatcatcg
     1081 cgctcgcggc attgcgggcg cggcggtaga tgtcgccatc gggcgtatag gcccagacat
     1141 ggcgatcggt aatcctctgc cagaaggcgc gtaatttttc gagccgctcg ctgcggggat
     1201 tgccggcgat gatcgcggcg ttgatggcgc caatcgaaac ccccgtcacc caatcgggtt
     1261 cgatattggc ctcgtgcagc gcctcgtaga cgcccgcctg ataggcgcca agcgcaccgc
     1321 cgccctgaaa gataagcgcg atttgatcgc agcgatcggg ccgccagccg ccggatttcg
     1381 atggcccggt gaagtggttg ccttgcttgg cgttgaaggg ggcttttgcg tccaaaattg
     1441 acctctcacc gcgcaggcta ggcaggcggg attgcaggaa tgtgacgctg ccgcgtcaca
     1501 ccattttcaa ggtaaacagg cagaagcaga gcgctgccgt ggcatttaac ttgctgcgtg
     1561 cctcagctcc agggacctga ccatgcgcgc tgaagatatc ctgcatttgc cgtccatgcc
     1621 gcttgccagc ccaagctatc ccaaggggcc gtatcgcttc gtcgaccgcg aatacatggt
     1681 catcacctac gagaccgacc ccgagatcat ccgccatcaa ttgccggaac cgcttgagcc
     1741 gcttgaccag ccgctcgtca gctacgaatg gatcaacatg ccggattcct ccggtttcgg
     1801 cagctacact gaaagcggtc tcgtcatccc gtgcaagttc cagggcgaag acgtgaactt
     1861 cgtcagccag atgtatctcg acgacgatcc gccgatcgcc ggaggccgcg aaatctgggg
     1921 cttcccgaag aaatatgcgc atccgaagct caaggtcttc aaggacacac tgaccgggac
     1981 tttgcattat ggcgaacagc tcgtcgccat gggcacgatg ggctacaagc acgaagccat
     2041 ggccggcaac ggcgtcggca cgacggcgat cctgtccaag acgcagatca atctcaagat
     2101 catcccgggg gtcgacggcg gtcacggcat ctgccagctt gtcgcctata acctcaccga
     2161 gattacggta aaaggttcgt ggaccggccc ggcgcggctg catctcgtgc cgcatgtgaa
     2221 tgccccggtc ggcgacttcc cggtacgcca tattgtcggc gcccgccatt tcatcgccga
     2281 cctgagcctg ccttatggcc gcgtcgtcca tgattatctg aagccataaa ttagctcata
     2341 tcaatgcctt atctgtgtca gccgcgggca gggccgcctg cggctggcgc gcgcatagtc
     2401 agagccaaaa atccgcgcta gagcatgtgc cccgcgctgg cgttggccag ggcgcccacc
     2461 cggatttttg cggaactctc gatgtcagat tcaaaagtcg ctctcatcac cggcgtgacc
     2521 gggcaggatg gagcgcttct cgccgaattg ctgctcaaca agggctatat cgtccacggt
     2581 atcaaacgcc gttcctcgct catcaatacg gatcgggtcg atcatcttta tcaggacccg
     2641 catgaggcgg acgttcgctt ctccatgcat tacggcgatc tcaccgatgc gacgaacctc
     2701 atccgcatcg tgcaggaggt ccagccggac gaaatttata atctcgccgc gcagagccat
     2761 gtgcaggtga gcttcgagac gccagaatac accgccaatt ccgatgcgct ggggacgttg
     2821 cggctgcttg aagcgatccg catcctgaag ctcgtggaca agacccgctt ttatcaggcc
     2881 tcgacctcgg aattatacgg caaagtccag gcggtgccgc agagcgagac aacgcccttc
     2941 tatccgcgct cgccttacgc ggcggccaag ctttatgcct attggatcac ggtcaattac
     3001 cgcgaagcat acggtattca cgcctcgaac ggaattctct tcaatcatga aggtccgact
     3061 cgcgcggaga ctttcgtgac ccgcaagatc acccgtgcgg ttgcggccat tcaactcggt
     3121 ttgcaaaaca agctctacct cggcaatctc gatgccaagc gcgactgggg ctatgcgggc
     3181 gattatgtcg agggcatgtg gcgtattctg cagcagccgg ttgcggacga ttacgtcctg
     3241 gcgacgggcg agacccacgc ggtgcgtgaa ttcgtcgaac gcgccttcgc ggaaatcggt
     3301 atcgaaatcg cgtggcgcgg tgccggcgtc gaggagcaag gattcgaccg taaatcaggc
     3361 cgagttctgg tcgagatcga cccggaatat ttccgcccga cggaagtcga tctgctcttg
     3421 ggcgaccctt cgaaggcacg cgacaagctc ggctgggttc acaagacgac ttttcccgaa
     3481 ttggtgaaag aaatggttgc gagcgatctc gtcctcatga agaaacgcgg ccatatataa
     3541 cccaaccgat ttgggacgga gcaattcacg catgaagccc tcgcgcattt tcgtggcagg
     3601 acatcgcggc atggtcggcg ccgcgctcat gcgccg
//
LOCUS       DASWOG010000515         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_67097,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000515 DASWOG010000000
VERSION     DASWOG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67097"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1455)
                     /locus_tag="VGB93_10605"
     CDS             complement(130..1455)
                     /locus_tag="VGB93_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0146574.1"
                     /translation="MSQAHQNAPLTPSVSPAKPAGPAVTSAKLANGLEIVVIPDHRAP
                     VVTHMVWYRNGSADDPVGKSGIAHFLEHLMFKGTKDHPAGQFSELIADVGGQENAFTS
                     NDYTAYFQRVPKDHLRTCMDYEADRMQNLVLTDEVVAPEREVVLEERRMRTDSDPSDL
                     LSEAVQSALFTRHPYGLPVIGWADDIAGLGRADALAYYERFYTPENAILIVAGDVEPK
                     DVITLAGEVYGKIPARGEAPKRWRVGEPKAQARRQITLADEKVEQPSYQVCFLVPSYR
                     TAPRDAEALEVLAHYLGGGQTSLLFRTLVVEKKLAVSIGAYYGGTALDETRFYLYGMP
                     APGVSLETLDEEIASIIAGLAEKGVDAADIERAKSRLVAETIYAQDNQGAMAQWYGAS
                     LATGLSLDDIQSWPTRIEAVTADQVLKAAHWLDHQRGVTGFLLPAGRAA"
BASE COUNT          309 a          502 c          502 g          308 t
ORIGIN      
        1 gatctgctgg actttcgccg cgtgcgaact ggtcggcttc aatcccgtct tgagactgtc
       61 ggtcattttg catcccgttg ggcgtgatcg ccggaaaccc agttccgcgg cggtcgcaac
      121 atttgaaaat taagccgcac gtccggccgg cagcaaaaac ccggtgacac cgcgctggtg
      181 atcgagccaa tgcgccgcct tcagcacctg atcggccgtg acggcctcga ttcgcgtcgg
      241 ccagctctga atatcatcga gcgaaaggcc ggtggcgagg gaggcgccat accattgcgc
      301 catcgcgccc tgattgtcct gcgcatagat cgtctcggca acgagacgcg atttggcgcg
      361 ctcgatatcg gccgcgtcga cacccttctc ggcaaggccg gcaatgatgc tggcaatctc
      421 ttcgtccagc gtctcaagcg agacgcccgg cgccggcatg ccgtagaggt aaaagcgtgt
      481 ctcatcgagc gccgtgccgc cgtaataagc gccgatcgac acagcgagct tcttttcgac
      541 gacgagcgtc cggaacagaa ggctcgtctg gccgccgccg agataatgcg ccagcacttc
      601 cagcgcctcg gcgtcgcgtg gcgccgtcct ataggaggga acgaggaagc agacctgata
      661 ggagggctgc tcaaccttct cgtcggcgag cgtgatctgc cgacgcgcct gtgctttcgg
      721 ctcgccgacg cgccagcgct tgggcgcttc gccgcgcgcc gggattttac catagacttc
      781 gccggcaagc gtgatcacgt ccttcggctc gacatcgccg gcaacgatga gaatagcatt
      841 ttccggcgtg tagaacctct cgtaataagc caaggcatcg gcgcgaccga ggccggcgat
      901 gtcgtcggcc cagccgatga ccggcagacc gtaaggatga cgggtaaata gcgccgattg
      961 caccgcctcg ctcaggagat cactcgggtc cgaatctgtg cgcatgcggc gctcttccag
     1021 cacgacttca cgctccggcg cgaccacctc gtcggtcaga accaagttct gcatccggtc
     1081 ggcttcataa tccatgcagg tgcgcagatg gtctttcggc acgcgctgga aataggccgt
     1141 gtaatcgttc gaggtgaagg cattctcctg gccgccgaca tcggcgatga gttcggagaa
     1201 ttggcccgcc gggtgatcct tcgttccttt gaacatcaga tgttcgagaa aatgcgcgat
     1261 acccgacttg ccgaccggat cgtcagccga cccgttgcga taccagacca tatgggtgac
     1321 gacgggggcg cgatgatccg ggatcaccac gatctcaaga ccgttggcga gcttcgcgct
     1381 ggtcacggcc ggacccgcgg gcttggcggg cgacaccgaa ggcgtgaggg gcgcgttttg
     1441 atgagcctgg gacattcgtc tgctttctca aagagaggca atggctaagc ttacgccatg
     1501 cccggaatct cgaataggtc acggcaggat ttgcgcgaaa accgctcagc gctttttcgc
     1561 aaggccgccc gtgaggctac cccggcccca tataggaagc aatttgcggc gcgcgcaggg
     1621 g
//
LOCUS       DASWOG010000516         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_177198,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000516 DASWOG010000000
VERSION     DASWOG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177198"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGB93_10610"
     CDS             <1..556
                     /locus_tag="VGB93_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0146575.1"
                     /translation="PVSGPSPRWNLMFQKPLLLPWLDVAGNVALGLRFAGRSKEAPAR
                     VAQMLDIVGLHGYGARPVTELSGGQQQRVALARSLATDPEILLLDEPYASLDPVTRAA
                     LRAEVRAIAAELGLTLLLVTHDVDDALGLAERVIVMAPNPGRLIADVVLASTPARGLR
                     AHLLGLLENDRETAGETLSVPVLQ"
     gene            537..1778
                     /locus_tag="VGB93_10615"
     CDS             537..1778
                     /locus_tag="VGB93_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0146576.1"
                     /translation="MCQYCNNLDYHATPDAPQSFTRRRTLDVLAAGGLATLLATMPRQ
                     ARAATTPDEPVRIGYLPITDATALLVAHAKGFFQDEGLNVEKPVLMRGWAPLVEAFAA
                     GKFNLVHLLKPVPVWMRYNNHFPVKIVAWAHTNGSAIVVGGDSKIHSFADFGGKQIAV
                     PFWYSMHNIVLQLALRQAGITPVIRPQGAPLAPHECNLQTLPPPDMPAALAARKIDGY
                     TVAEPFNAMGEIAVHARILRFTGDMWKNHPCCVVCMHEADTVQRPEWSQKILNAIVRA
                     EIYASQHKVEVATLLSRDGAGYLPMPAPVVTRAMTLYTQNAEYLSDGAIEHPQWHNGR
                     IDFQPWPYPTATTLIVEEMKKTLVAGDRTFLDKLDPAFVTKDLVNYDFVRAALKLHPE
                     WKTDPSVNAADPFARHEVLAL"
     gene            1805..>2525
                     /locus_tag="VGB93_10620"
     CDS             1805..>2525
                     /locus_tag="VGB93_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0146577.1"
                     /translation="MVRRQWHGVSLRFAAPLIGLLLLLAIWALGGWVLTQSPQGTAFA
                     GFAPRPTFVSLARMIANGSLWQAFVPSIRRIGLGLAFAMLIGAPVGVLVGHSAIFRQL
                     THVPFQLLRMISPLAWMSIAILAFPTWEGAIVFLIAAASVWPIIFATANGLQKLDPDW
                     VKLAKNLGARPWNLLTIVILPAILQDILVGIRLALGVAWIVLVPAEYLGVTSGLGYAI
                     NDARDTLEYDRLAAFIVAIGVI"
BASE COUNT          443 a          789 c          762 g          531 t
ORIGIN      
        1 gcctgtctcc ggcccgtcac cgcgctggaa tctgatgttt cagaaaccgc tgcttttgcc
       61 gtggctcgac gttgcgggga atgtcgcgct cggcctgcgt ttcgccggtc gctcgaaaga
      121 ggccccggcg cgagtagcgc aaatgctcga tattgtgggg ctgcatggct acggcgcgcg
      181 tccggtcacg gaattgtccg gcggccagca acagcgtgtc gccttggcgc gatcgctggc
      241 gaccgatcca gaaatccttt tgctcgacga accttatgca tctctcgacc cggtgacgcg
      301 tgccgcgttg cgcgccgaag tacgcgcgat tgccgccgag cttggcctca cgctgctgct
      361 cgtcacccat gatgtggacg acgctctcgg tcttgccgaa cgcgtcatcg tcatggcgcc
      421 aaatccaggc cggctgatcg ccgacgtcgt tttggcgtca acgccggcac gcggcttacg
      481 tgcccatctt ttggggcttc tggaaaatga ccgcgaaact gcgggggaga cattaagtgt
      541 gccagtattg caataatttg gattaccacg ccacgccgga tgcgccgcaa tcatttacgc
      601 gacggcggac gctggacgtg cttgccgccg gcggactcgc aacgttgctc gccacgatgc
      661 cgcggcaagc gcgcgccgca acgacgccgg acgagccggt ccgtatcggc tacctgccga
      721 tcactgatgc gaccgcactg ctcgtcgccc atgctaaagg tttctttcag gacgaaggac
      781 tgaacgtcga gaagccggtt ctgatgcgcg gctgggcgcc gcttgtcgag gcatttgccg
      841 ccggcaagtt caacctcgta catctgctga agccggtgcc ggtctggatg cgctacaaca
      901 atcacttccc ggtcaagatc gtggcctggg cgcatacgaa cgggtccgcc atcgttgtcg
      961 gcggcgacag caagatccat agcttcgccg attttggcgg caagcagatc gcagtaccgt
     1021 tctggtattc gatgcataat atcgtgctgc aattggcgct gcgacaggcg gggatcacgc
     1081 cggtcatccg gccgcagggc gcaccgctcg caccgcacga atgcaatctg caaacgctgc
     1141 cgccgcccga catgccggcg gcgctcgcgg cccgtaagat tgacggctat acggtcgccg
     1201 aacccttcaa cgctatgggt gagattgccg ttcacgcgcg catcctgcgc tttaccggcg
     1261 atatgtggaa gaaccacccg tgttgcgtcg tctgcatgca cgaggccgat acggtccagc
     1321 ggccggaatg gtcgcagaag attctgaatg ccatcgttcg cgctgaaatc tatgcctcac
     1381 aacacaaggt cgaggttgcc actctgctgt cgcgcgatgg cgctggttat ctgccgatgc
     1441 ccgcgcccgt cgtgacgcga gcgatgacgc tctacacgca aaatgccgaa tatttgagtg
     1501 atggggcgat cgagcatccg caatggcaca acggccggat cgactttcag ccgtggccct
     1561 atccaacggc gacgacgctg attgtggaag agatgaaaaa aacgctcgtc gccggagacc
     1621 ggacgtttct cgacaagctc gaccccgctt tcgtcaccaa ggacttggtg aactacgatt
     1681 tcgtccgcgc cgcgctgaag ctccatcctg aatggaagac ggacccgagc gtcaatgccg
     1741 ctgatccctt tgctcgtcac gaggttctgg cactgtgacg agtatcgccg tgcaacctga
     1801 acgcatcgta aggcgccaat ggcatggggt ttcccttcgc ttcgcagcgc cgttgatcgg
     1861 gctgttgctg cttctggcga tttgggcgct cggcggttgg gtgctgacgc aaagtccaca
     1921 aggcacggct ttcgcaggct tcgcgccgcg tccgacgttt gtcagcctcg cgcgcatgat
     1981 cgcgaacggc tccttgtggc aagcatttgt accaagcatc cgccggatcg gcctcggcct
     2041 cgcttttgca atgttgatcg gtgcgccggt cggtgttctt gtcggccaca gcgcgatctt
     2101 ccggcagctt acgcatgtcc cgtttcaatt gttacggatg atcagcccgc ttgcctggat
     2161 gtccattgcg atcctggcgt ttccaacctg ggaaggtgcc attgtctttc tgatcgcggc
     2221 cgcctccgtc tggccgatca tcttcgccac ggcgaacggg ctgcagaaac tcgatcctga
     2281 ctgggtcaaa cttgcaaaga acctcggcgc caggccgtgg aacctgctga caatcgtaat
     2341 tctgccggcc attctgcagg atattcttgt cggcatccgg ctggcgctgg gcgtcgcctg
     2401 gatcgtcctc gttcccgccg aatatctcgg cgtgacgagc ggcctcggct acgcgatcaa
     2461 cgacgcgcgc gacacgctcg aatatgaccg gctcgccgcg ttcattgtgg ccattggcgt
     2521 catcg
//
LOCUS       DASWOG010000517         6084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_67128,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000517 DASWOG010000000
VERSION     DASWOG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67128"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..2265
                     /locus_tag="VGB93_10625"
     CDS             4..2265
                     /locus_tag="VGB93_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0146578.1"
                     /translation="MSEATNALDPKVPRRTPKRLIFGRAVVVLAIATALISFLIFAGY
                     TPIAPTDVVVLRLFVLNLVCILALVGFVMVEAWSIIAARRRQAAGARLHIRIVGLFTI
                     IAIVPAILMALVGSITLDRSLNPTFLRDVRGFILRTGEAAQFFQEGQCRVLLREAQLT
                     ASDLDRGVSLFKSDRQLFHEFFSSRIEALGFGAAAMIHPDGKIDDKVVASGREGAPIV
                     RPQSSDFADASKNEPLCMILDEGRTFVALRKLQAFDDAYLYVARPVGPFGVEFRHQAR
                     HLITFYDMFDGYRHNIQIAFATMYVLIALIMLLAATWVGLAFANGLVAPIRRLITAAD
                     EVGSGNLDAHVAVNPSEGELGHLGETFNKMTSEIRLQQNRLIAASTLIDERRLFTEAV
                     LSGVPVAVIGVDGKGQITVVNPSAEKLIANEDGRGAVGRIIDDVLPEVAPVLHDARGT
                     NGRLARGQISVNRGGRDRTFNLAVTTELGGGTEKNYVITLDDISDLVTAQRTAAWADV
                     ARRIAHEIKNPLTPIQLSAERLKRKYGRLIVDDRDIFDQCIDTIVRQVDDIKRMVDEF
                     SAFARMPRAQLVRDDVAECLRHVIFLMRVGHPDIEFADALPQSPLLANFDRRLLSQAV
                     TNITKNATEGIAAQAEIEPMKGKIAISLDVTREGLVEILIADNGRGFPKENRHRLLEP
                     YMTTRAEGTGLGLPIVAKILEDHGGGIELLDAPGGRGACVRLYFPKGDTSHGVNEFAG
                     EERTTALIETAKW"
     gene            2292..3662
                     /locus_tag="VGB93_10630"
     CDS             2292..3662
                     /locus_tag="VGB93_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0146579.1"
                     /translation="MPSDILIVDDEADIRDIVSGILSDEGHGTRTAKNAEEALNAIEA
                     RRPHLAFLDIWLQGSHLDGLQLLQLVKTAHPTLPIVMISGHGNIETAVSAIKMGAYDF
                     IEKPFKADRLVLVAERALEASRLKRELSELRTRSGVANRIVGKSSVIHQLHQVIGRVA
                     PANSRVMITGAPGSGKELVARSIHDASPRANAPFVVINSATITPEMMEIELFGVESRD
                     GQQSRKVGALEEAHGGTLYLDEIADMPKETQGKILRVLVDQNFLRVNGATRVNVDVRI
                     LSSSSRDLPALIADGALREDLFHRLSVVPIRVPSLSEHREDIPELIEFFMDQISATTG
                     LPKRNIADEAMAVLQSHDWPGNIRQLRNNVERLMILAAGDPSTELTSDMLPSEIGALV
                     PSTPNGSGGERLMSLPLRDAREVFEREYLIAQINRFGGNISRTAEFIGMERSALHRKL
                     KSLGID"
     gene            3675..4535
                     /locus_tag="VGB93_10635"
     CDS             3675..4535
                     /locus_tag="VGB93_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEY0146580.1"
                     /translation="MGRIAYVNGRYLPQAQATVSIEDRGYQFGDGVYEVCEVRGGALI
                     DEERHLARLDRSLRELKMSAPLAPKALSRVLREVLTRNKIRDGYVYAQVTRGVAPRDH
                     AFPARAVRPSLVVTAKAIDPAKGAQQAAKGIKVISLPDIRWKRPDIKTINLLPNVLAR
                     QAAKEQGAYEAWLIDDEGMVVEGAASNAWIVTPEKIIVTHQVDQSILRGVTRTTLLDL
                     IAAKGYRLEERRFSLDEAKYAEEAFITGATTLVMPVIAIDDTLIGGGKPGPIATSLRA
                     QFHNFARATV"
     gene            4734..4988
                     /gene="hfq"
                     /locus_tag="VGB93_10640"
     CDS             4734..4988
                     /gene="hfq"
                     /locus_tag="VGB93_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0146581.1"
                     /translation="MAAERTQNLQDTFLNFVRKNKVPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGHPIQMFEPGEETPAGS"
     gene            4985..>6084
                     /gene="hflX"
                     /locus_tag="VGB93_10645"
     CDS             4985..>6084
                     /gene="hflX"
                     /locus_tag="VGB93_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0146582.1"
                     /translation="MTSRDKDANTASATRTRAFVIGPYLNERHKRRADAAVQGIDNAR
                     SAEARIEEARGLALAIDVDVVDAWLVTLTQIRPATYMGPGKVEEIAERTKAEAIDLVV
                     MDCGLSPVQQRNLEKAFACKVIDRTGLILEIFGRRALTREGTLQVELAHLTYQKSRLV
                     RSWTHLERQRGGFGFLGGPGETQIETDRRLIQERITRIERDLEKVKKTRGLHRKSRSA
                     VPYPVVALVGYTNAGKSTLFNRLTRAYVLAENMLFATLDPTLRALKLPRGSEIILSDT
                     VGFISDLPTMLIAAFKATLEEVLEADVILHVRDIAHEDSEAQRGDVEKVLVELGIDIE
                     QPGRVLEVWNKIDALDSESALALPNTARRRPA"
BASE COUNT         1229 a         1802 c         1826 g         1227 t
ORIGIN      
        1 cggatgagtg aagccacgaa tgcgcttgac cccaaggttc cgcgcagaac gccgaagcgc
       61 ctgattttcg ggcgcgcggt ggtcgtgctc gcgatcgcca cagcgctgat cagcttcctg
      121 attttcgccg gttacacgcc gatcgcgcca accgacgtcg ttgttctgcg gctcttcgtc
      181 ctcaaccttg tttgtattct ggcgctcgtc ggcttcgtga tggttgaagc ctggtcgatc
      241 atcgctgcgc ggcggcgaca ggcggctggc gcacggctgc atatccggat tgtcgggcta
      301 ttcacgatca tcgccattgt cccggctatt ctcatggcgc tcgtcggctc gatcacgctc
      361 gaccgcagcc tgaatccaac attcctgcgc gacgtgcgtg gcttcatcct gaggaccggc
      421 gaggcggcgc aatttttcca agagggtcaa tgccgcgtgc ttctgcgcga ggcacaattg
      481 accgcctccg accttgatcg cggcgtctcg cttttcaagt cagatcggca attgttccac
      541 gaattcttct catctcgcat cgaggcgctc ggcttcggtg cggcggcgat gatccatccg
      601 gacggcaaga tcgacgacaa agtcgttgcc tcgggcaggg agggagcgcc aatcgtgcgg
      661 ccgcaatcca gcgattttgc tgacgcgagc aagaacgaac cgctgtgcat gattttggac
      721 gagggacgta cgttcgtcgc gctgcgtaag ctgcaagctt ttgacgatgc gtatctctac
      781 gtcgcgcgcc ctgtcggccc gttcggcgtc gagtttcggc atcaggcgcg ccaccttatc
      841 actttctacg acatgttcga cggctatcgg cacaacatcc agatcgcttt tgcgacaatg
      901 tatgtgttga tcgcgttgat catgttgctc gccgcgacct gggtcggctt ggcgttcgcc
      961 aatggtcttg tcgcgccgat ccggcgcctt atcaccgctg ccgatgaggt cggctccggc
     1021 aaccttgacg cccatgtcgc cgttaaccca tccgaaggtg agttgggcca tctcggcgaa
     1081 accttcaata agatgacttc cgagatccgc ttgcagcaga accgcctgat cgccgcgagc
     1141 acgttgatcg acgaacggcg gctattcacc gaggcagttc tctcgggcgt gccggtggcc
     1201 gtcatcggcg tggacgggaa gggtcagatc acggtcgtca atccgtctgc ggagaagctc
     1261 attgcgaatg aagacggccg cggcgccgtc gggcgcatca tcgatgacgt gctgccggaa
     1321 gtggcgcccg tgcttcacga cgcgcgcgga acgaacgggc ggctggcgcg cggtcagatt
     1381 tccgtcaaca gaggcgggcg cgaccgcacc ttcaaccttg ccgtgacgac cgaactcggc
     1441 ggcggcaccg agaaaaatta tgtcatcacg ctcgacgaca tcagcgatct cgtcaccgcg
     1501 caacgcaccg cagcctgggc ggatgtggcg cgacgcatcg cgcatgagat taaaaatcca
     1561 ctgacgccga tccagctttc agccgaacgg ctgaagcgca aatacggccg ccttatcgtc
     1621 gatgaccgcg atattttcga ccaatgcatc gatacgatcg tgcgccaggt tgacgacatc
     1681 aagcgcatgg tcgacgaatt ttccgccttt gcgcggatgc cgcgggcgca actcgtgcgg
     1741 gacgatgttg ccgaatgcct gcgccatgtg atcttcctca tgcgcgtcgg ccacccggac
     1801 atcgaatttg ccgatgcgct gccgcaatcc ccgctgcttg ccaatttcga tcggcggttg
     1861 ctctcgcaag ccgtcactaa tatcaccaag aacgcgacag agggcattgc agcacaggcg
     1921 gagatcgagc ccatgaaggg caaaatcgcg atttcgctgg atgtcacgcg ggaggggctt
     1981 gtcgaaattc taattgccga taatggtagg ggctttccga aagaaaatcg gcatcgcctg
     2041 ctcgaaccct atatgacgac ccgagccgag ggcactgggc ttggcctgcc gatcgttgcc
     2101 aaaatcctcg aggatcacgg cggcggcatt gaattgcttg atgcgccggg tggccgggga
     2161 gcctgcgtcc ggctttattt tccaaaaggt gacacttcgc acggcgtcaa tgagttcgcc
     2221 ggggaagaac gtacgactgc cctcattgag actgccaagt ggtaacgcta aaagcgagag
     2281 cttgaacctt aatgccaagc gacattctca tcgtcgatga cgaggctgat atccgtgaca
     2341 ttgtgtcggg cattctgtcc gacgaaggtc acggcacccg cacggcaaaa aacgccgagg
     2401 aggccctcaa cgccatcgag gcgcggcggc cgcatcttgc cttcctcgac atctggctgc
     2461 agggctcgca tctcgacggc ttgcaactgc tgcaactcgt gaagactgcg catccgacat
     2521 tgccgattgt gatgatctcg ggtcacggca atatcgagac ggccgtgtcg gcgatcaaaa
     2581 tgggcgccta cgacttcatc gagaagccgt ttaaggccga caggcttgtg ttggtcgcgg
     2641 agagggcact cgaagcttcg cgtctcaagc gtgaattgag cgaattgcgc acgcgctccg
     2701 gcgtcgccaa tcgtatcgtc ggcaagtcga gcgtgatcca tcaattgcat caggtcatcg
     2761 gccgtgtcgc gccggcgaat tcacgcgtga tgatcaccgg tgcgcccggc tccggcaagg
     2821 aactcgttgc gcgcagtatc catgacgctt ctccgcgtgc aaatgccccg tttgtcgtca
     2881 tcaattcggc gacaattacg ccggagatga tggaaatcga gcttttcggt gtcgagagcc
     2941 gggatgggca gcagagccgt aaggtcggtg cgctggaaga agcgcatggc ggcacgcttt
     3001 atctcgacga aatcgccgac atgccaaaag agacgcaggg caagatcctg cgggttctgg
     3061 tcgatcagaa tttcttgcga gtcaacggtg cgacccgcgt gaatgtcgac gtgcgcatat
     3121 tgtcctcgag ttcgcgcgac ctcccggcgc tgatcgccga tggggctttg cgcgaggact
     3181 tgttccaccg cctctcggtc gtgccgatcc gagtgccgtc gctttccgaa catcgcgagg
     3241 acattccgga actcatcgaa tttttcatgg accagatatc ggcgacgacg ggtttgccca
     3301 aacgcaatat cgccgatgaa gccatggccg tcctgcaatc gcacgattgg cccgggaaca
     3361 tccgccagct ccgcaataac gttgagcgtc tgatgatcct cgcggccggc gatccgagta
     3421 ccgaattgac atccgacatg ttgccgtcgg agatcggcgc tttggtacct tcgacgccca
     3481 atggttcggg cggcgagaga ctgatgagcc tgccgctgcg cgacgcgcgc gaagtcttcg
     3541 agcgcgaata tctgatcgct cagatcaatc gtttcggcgg caatatttcg cgcacggccg
     3601 aattcatcgg catggaacgc tcggcactgc atcgcaaatt gaagtcgctt ggaatcgatt
     3661 gagacggggg cggggtgggg cgcatcgcat atgtaaatgg tcgctacctg ccgcaggccc
     3721 aggcgaccgt ctcgatcgag gaccgcggct atcagttcgg cgatggtgtc tatgaggtct
     3781 gcgaggttcg tggcggcgcg ctgatcgatg aggagcgcca tctcgcgcgc ctggaccgat
     3841 ccttgcgcga actcaaaatg tcggcgccct tggcaccgaa ggcgctgtcg cgggttttgc
     3901 gcgaggttct gacgcgcaac aaaatccgcg acggctatgt ctacgctcag gtgacgcgcg
     3961 gcgtcgcccc gcgggatcat gctttcccag cgcgggcggt gcggccgagc ctcgtggtga
     4021 ccgccaaggc gatcgacccg gcgaaaggcg cccagcaggc tgccaagggc atcaaggtca
     4081 tatctctgcc cgacatccgc tggaaacgtc ccgacatcaa gacgatcaac ctgcttccca
     4141 atgtgctcgc gcggcaggcg gcaaaagagc agggcgctta tgaagcgtgg ctcatcgacg
     4201 acgagggaat ggtcgtcgaa ggcgccgcct ccaacgcttg gatcgtgacg cctgagaaga
     4261 tcatcgtgac gcatcaagtc gatcagtcga ttctgcgcgg ggtgacccgg accacattgc
     4321 tcgacttgat cgcggctaag ggctaccggc tcgaagaacg ccgtttcagc ctcgatgaag
     4381 ccaagtacgc ggaagaagcc ttcatcaccg gcgcgacgac gctggtgatg cctgtcatcg
     4441 cgatcgacga tacgcttatc ggcggcggca aaccggggcc catcgcgacg agtttacggg
     4501 cgcagtttca caattttgct agagcaaccg tctaaaggac ggggcagcgg gagttggaat
     4561 ttggccctac atgccaagga taaagtccct cttgcagctt tgccacacga taaggtctaa
     4621 tgcacacgat cggctatcgc taaagctaag ctggcgctgg tccaccttaa gtcttttcgt
     4681 tgcgtcccgg gccacgttat taacgtggcc tcaaaaaaga acaggaagaa aaaatggcgg
     4741 ccgagcgcac tcaaaatcta caagacactt ttctaaattt cgttcggaaa aataaagtcc
     4801 cgctgaccat ttttctggtc aatggcgtca agttgcaagg cgtcgtgacg tggttcgata
     4861 atttctgcgt cttgctacgg cgcgacgggc attcgcagct tgtttacaag cacgcgattt
     4921 cgaccatcat gccgggtcac ccgattcaga tgttcgaacc cggcgaagag acgccggccg
     4981 gttcgtgacg tcccgagaca aagacgcaaa tacagcatcg gcgacgcgga cccgcgcctt
     5041 cgtgattggg ccttatctca acgagcggca caagcgtcgc gccgatgctg ctgtccaggg
     5101 gatcgacaat gcacggtcgg cggaagcgcg gatcgaggaa gcgcgcggtc ttgcgcttgc
     5161 gatcgacgtt gacgtcgttg atgcctggct cgtcacgctc acgcaaattc gccccgcgac
     5221 ctatatgggg ccgggcaaag ttgaagagat cgcggagcgc accaaggccg aggcaatcga
     5281 tcttgtcgtc atggattgcg gactttcgcc ggttcaacag cgcaaccttg agaaggcgtt
     5341 tgcctgcaag gtcatcgaca ggacggggct tattctcgaa atctttggcc ggcgcgccct
     5401 gactcgtgaa ggcacgctgc aagtcgaact tgcgcatctg acctatcaga aatcccgact
     5461 cgtacgctcc tggacgcatc ttgagcgcca acgcggcggc tttggcttct taggcggccc
     5521 gggtgagacg cagatcgaaa ccgaccggcg actcattcag gagcgcatca cccgaatcga
     5581 gcgcgacctt gaaaaggtga agaaaacacg cggcctccac cgcaagagcc ggagcgccgt
     5641 gccttatccc gtcgtcgcgc tcgtcggcta tacgaatgcc ggcaaatcaa ccttgttcaa
     5701 ccgactgaca cgcgcctatg tgctcgcgga aaacatgctc ttcgctacgc tcgatccgac
     5761 attgcgggcg ctgaagcttc cgcgcggaag cgagatcatc ctgtccgaca cggtcggctt
     5821 catttccgat cttccgacca tgctgatcgc tgctttcaaa gccactttgg aagaagtgct
     5881 ggaggccgat gtcatcctgc atgtgcgtga catcgcacat gaagacagtg aggcgcagcg
     5941 cggcgacgtc gaaaaagtgc ttgtcgaact cggcatcgat atagaacagc cgggccgggt
     6001 cctcgaagtc tggaacaaga ttgacgcgct cgattccgag agcgctcttg cgctgcctaa
     6061 cactgcgcgc cgacgccccg ccag
//
LOCUS       DASWOG010000518        10527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_341932,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000518 DASWOG010000000
VERSION     DASWOG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341932"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..801
                     /locus_tag="VGB93_10650"
                     /pseudo
     CDS             37..801
                     /locus_tag="VGB93_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611807.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1320..1838)
                     /gene="msrA"
                     /locus_tag="VGB93_10655"
     CDS             complement(1320..1838)
                     /gene="msrA"
                     /locus_tag="VGB93_10655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0146583.1"
                     /translation="MAETERAVLAGGCFWGMQDLIRHRPDVLSTRVGYTGGDVKNATY
                     RNHGTHAEAIEIVFDPTRTTFRDILEFFFQIHDPTTLNRQGNDVGASYRSAIFYTNET
                     QKHVAEDTIADVNASGLWPGKVVTKVEPVGDFWEAEPEHQDYLVRLPHGYTCHFPRPN
                     WKLPHRAAATAK"
     gene            1949..2476
                     /gene="mug"
                     /locus_tag="VGB93_10660"
     CDS             1949..2476
                     /gene="mug"
                     /locus_tag="VGB93_10660"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEY0146584.1"
                     /translation="MMSDGLPDVLAPGLAVVFCGINPGAHAAAAGHNFVGRSNRFWRA
                     VHLAGFTPTELSAEEDQTFVRYGCGLTVAVARPTRRAEEITRDERSAGAAALRARIEH
                     YAPRTIAFLGKASFAAIMGQRHVTWGPQPARFGGAAVWVVPNPSGLNRGFSLDQLVEA
                     YGTLRRAVAKDLPSP"
     gene            2592..2897
                     /locus_tag="VGB93_10665"
     CDS             2592..2897
                     /locus_tag="VGB93_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY0146585.1"
                     /translation="MSEHCHHLHSIRDVTPRARGCEECLKTGDWWVHLRICRTCGHVG
                     CCDDSPNKHATAHFHATSHPIIEGYDPPEGWGWCYVEEVFVDLPDQTPQLGPIPRYI"
     gene            2945..4594
                     /locus_tag="VGB93_10670"
     CDS             2945..4594
                     /locus_tag="VGB93_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146586.1"
                     /translation="MPTDPYDRPAQTFPKLPPEMVARIKKYGQTDSFDGGAYLYNVGD
                     RDVDFFLILEGGVDVLESDGHGGHVLVVTHEASEFTGELDHLSGRAVFVCARAARPTK
                     VVRISSASFRKLASAEPDIGEIILRAFILRRVGLIQHAEGGTILVGSQRLSDTLRIQS
                     FLTRNACPHRFLDTDVDPDAAIALKYFHLEGNAQPVVIINARDVLQKPTNSELADALG
                     ITEHLDPDRIYDVVVVGAGPAGLAAAVYAASEGLDTVVIESMAPGGQAGSSSRIENYL
                     GFPTGISGQALSGRAQVQAQKFGARLAIARAAIGLDCGFPFRVHMEGRTAVTTRAVVV
                     ASGARYRRLDVEDLARFEGQGVHYAATPIEGGLCAGAEIVVVGGGNSAGQAAVYLSRK
                     ARHVHILVRGDGLAATMSDYLVRRIEESAQITLHTRCDITALIGDKYLEAVRWRDKDG
                     AETQVSTSNLFLMIGAVPNTDWLNGCVDLDPKGFVETGHIKAGEALTTPYVTSVPGIF
                     AVGDVRANSVKRVASGVGEGSVVVHAIHRWLAKQAATDPSN"
     gene            complement(4591..5808)
                     /locus_tag="VGB93_10675"
     CDS             complement(4591..5808)
                     /locus_tag="VGB93_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY0146587.1"
                     /translation="MDKIVRHQTPHGTAISDLSVWRATLSAFCTSLVGIGLARFAYAP
                     LLPAIINAHWFAPSAAAYLGAANLAGYLGGALLGRQLAVRLPVAIALRLMMILATAAF
                     FACAFPLSFLWFFAWRFVAGLSGGVLMVLAAPTVLAHVPAGRRGLAGGIIFMGVGIGV
                     IASGTLVPFLLRQSLTETWLGLGLLSLALTVVAWSGWPAGAPKAANIEPLQRPHHRGA
                     LKALYVEYALNAAGLVPHMIFLVDYIARGLGQGLQTGAQYWVLFGLGATIGPLLTWHL
                     ADRAGFGPALRLAYLLQAAAVVLPALGLGSVSLVISSVVVGAFTPGIVPLVLGRVHEL
                     LAHHPAEQKGAWSAATVSFATLQATAAYGLSFIFAQSGGNYRLLFVIGTIALLAALAI
                     DLIAAVLIRGRAD"
     gene            complement(5869..6558)
                     /locus_tag="VGB93_10680"
     CDS             complement(5869..6558)
                     /locus_tag="VGB93_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0146588.1"
                     /translation="MKILMVLTSHDKLGNTGRKTGFWLEELAAPYYVFKDAGADITLA
                     SPKGGQPPLDPKSNEPNFQTDLTRRFEADADAKAQLATTLRLDSVKSEDFDTVFYPGG
                     HGPMWDLAEDPNSIKLIESLLAAGKPVALVCHAPGALRHVKTPEGRPLVEGKQVTGFT
                     DTEEEAVGLTKVVPFLVEDELKAEGGIFSKTADWGVHVVSDGLLTTGQNPASSGPAAK
                     VLLEKLAETAK"
     gene            6751..7845
                     /locus_tag="VGB93_10685"
     CDS             6751..7845
                     /locus_tag="VGB93_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0146589.1"
                     /translation="MADLFSPGKIGAIPVSNRVAMAPLTRARADMDGVHTPLAIEYYR
                     QRASAGLIISEATNISRQGRGYAFTPGIYTDAHVRAWRPVTEAVHEAGGHIVLQLWHV
                     GRISHDSLQEGGQPPVAPSAIQAGGLTYIETGFERPSMPRALRTDEIPGIIDDYRNAA
                     RKAKDAGFDGIEVHSANCYLLEEFIRDITNKRDDAYGGSIENRTRLTFEVARAVAEIW
                     GPERVGVRLSPLTKSAGDTPLDSDPQTTYGFLAERLGTLNLAYLHCIEGQTRGDNAKA
                     EFDFQALHRAFGGAYIANNGYDRELAIEAIATHHADMVAFGRPFIGNPDLVERLQRDE
                     PLATAPQETFYGVGAKGYIDFPTFAAASTS"
     gene            7881..8249
                     /locus_tag="VGB93_10690"
     CDS             7881..8249
                     /locus_tag="VGB93_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY0146590.1"
                     /translation="MAEKSSRVQIKRVYEPASPADGARVLVDRIWPRGLSKAHADVKL
                     WLKDIAPSTDLRKWFGHEPERWPEFRTRYWAELSKSEEAVTQLLDVVKHGHTTLVYAA
                     HDELHNNALALLEYLAAHRH"
     gene            complement(8593..9837)
                     /locus_tag="VGB93_10695"
     CDS             complement(8593..9837)
                     /locus_tag="VGB93_10695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0146591.1"
                     /translation="MADNGAHISSSTSADLASIDAAIQSRNWLLRFPGKLEAAFERDT
                     GAERCRDLIVRAYIGIVVYDLFAIADYWATPKLFWTAFWVRAAFFTPVALILTAVLHT
                     RPRAFVRESLVCVGGGAISVATIIFLMAASGTAPQATLYESMILVVLFLSVVQRTRFS
                     FLAPTCLASIVGHVLAVAHFYHYATGQQVAINMVFSAAVIFALIASYTMERDLRLHYL
                     LSQRGLAQNSELDKMTRRDSLTGLGNRRALEETLAACEQFDFAVELSIVLLDIDHFKL
                     FNDSAGHQAGDLCLKRVAGIIQAELRGQADHAFRFGGEEFIIVLPQTPMAKALVVAER
                     MRRAIESAGIPHPALDSAAYVTASFGVASGQPAFDLTANGIVANADSCLYAAKRNGRN
                     QVWPGSPAGVGPDTGTSTSLNA"
     gene            10078..10479
                     /gene="msrB"
                     /locus_tag="VGB93_10700"
     CDS             10078..10479
                     /gene="msrB"
                     /locus_tag="VGB93_10700"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0146592.1"
                     /translation="MDVKDYPVTHSEAEWRKLLTPEQYQVMRGHGTERPGSCALNFEK
                     RAGSFTCAGCDQPLFVSKTKFESGTGWPSFNDPVPGSVETSVDNSYGMERVEAHCSRC
                     GSHLGHVFPDGPPPTHLRYCINGVAMNFHPE"
BASE COUNT         2179 a         3103 c         3189 g         2056 t
ORIGIN      
        1 gaccctgagg tgcctgtttc ttaggaaggc cgcggcctac ttcgcgaagg agtcgacatg
       61 aagttcggct ttatcgtgaa gcaccgaacc gtctggcccg gtggcatggc tctgcgaagc
      121 gctgggtgtc tcaagatctg gctttcatgc ctggctgaaa cgaggaccga gcatgtgcgc
      181 ccggatggat gaaactcttc taccgatcat caaatcgagc tttgtgacga gcgcccgcac
      241 ctatggcgcc cgaagagtct ggcgggatgt tctggaaaca ggcctgtcct gtggcttgca
      301 caagatcgag cgcctgatgc gcgccagtgc cttgcgagcg cagcctcttc gctcgtccgc
      361 ccggcggcga tagcttgcca gaaacggcag agattctcac gctgccaacc cggcggatga
      421 cccggcgagc gcagcctctt acgcatgcac cggtccgaag ctcgtttgta aaccttcatc
      481 gcaacctcca acttcgtgat gttgcgacga ccggttgaat ccacccagta caccagcgag
      541 cagttcgaga agctgatggc cgataatggc atcgactgtt cgatgagccg ctcgggaaac
      601 gtctgggaca acgcggcgat ggagagcttc ttctcatcgc tgaagaccga acggatcggc
      661 cataaggtct atcgcgatcg cgatgccgca agggccgacg tgttcgatta catcgagagg
      721 ttctacaatg cggttcgcag gcactcgacg ctgggctatc tcagcccggt tgagttcgaa
      781 cggaaggtgg gattagctta accgcctgtc catcaaaccg gcagcaggcc aagcagggac
      841 tttaggccgt aagcgtgcca ttgccagccc atcttaaatg catcgcctgg ttttgagggg
      901 ccagtttcat aaatggcaag cgcgctctct ttgatcattt acggaaagcc cgtcagcagc
      961 ccagcttaag acggcggcca tctctatcat ttggtcaatt cggtcaagga gcaggctaat
     1021 gattcagtag acgggagccc caaggggaac gtcaccgaca acagcagttt tgacaatttg
     1081 cctgtgtaat cgggcgaatt ggcgccgagt ctgacttctt tgtgactact tctctaggag
     1141 tcgaaatagt ccgcgtctcg agtcgagctt ttaagtgcga cacttcatat aagtcattga
     1201 attatatgat gcggatattc gtcgtagagt cgaaattcag tgttagcaca ccgagctttc
     1261 accgctggcg tcgccagaac gcgggggcga cgccccgcgt tgttttataa cttctacgct
     1321 cacttcgccg tcgctgcagc acggtgcgga agtttccagt tcggtcgcgg gaaatggcag
     1381 gtatagccgt ggggcaaacg gaccaaatag tcctggtgct caggttctgc ctcccagaaa
     1441 tcgccgacag gttcgacttt cgtcacgact ttgccgggcc agaggccgga ggcattcacg
     1501 tcagcgatcg tgtcttcggc gacgtgcttc tgcgtttcat tcgtatagaa gatcgcagac
     1561 cgatagctcg cgccgacatc attgccttgc cggttcaacg tcgtcggatc atggatctgg
     1621 aaaaagaact ccagaatgtc gcgaaacgtc gtccgggtgg ggtcgaatac aatttcgatc
     1681 gcttccgcat gcgtgccatg attacgatat gtcgcgttct tcacatctcc gccggtataa
     1741 ccgacgcgag tcgagaggac atccgggcgg tgccggataa ggtcctgcat gccccagaaa
     1801 cagccgccgg caagaacggc gcgttcggtc tcggccatgt cgagtctcct tcattttcag
     1861 catcttcgct cggggtgagc gcggagagtg ccggcttcgt cacgtcttgt atagatattc
     1921 cggcgcgttt cgcgttcaag gacagcctat gatgagcgat ggtttgcccg atgtcctcgc
     1981 gcccgggttg gcggtcgtct tctgtgggat caaccccggc gcgcatgccg cggctgcggg
     2041 ccacaacttt gtcggccgca gcaaccgctt ctggcgcgcg gttcatctgg ccggttttac
     2101 cccgaccgaa ctttccgccg aagaagatca gacttttgtc agatatggat gcggcctgac
     2161 ggtcgcggtc gcgcggccga cccgaagggc tgaggagatc acgcgcgacg agaggtccgc
     2221 tggcgccgct gcacttagag cgaggatcga gcattacgca ccccgaacca tcgcctttct
     2281 tggcaaggcg tccttcgccg caatcatggg gcagcgtcac gtgacctggg gcccgcaacc
     2341 ggcgcggttc ggcggcgcgg cggtctgggt cgtaccaaac ccaagcggtc ttaaccgcgg
     2401 gttcagtctg gatcagctgg tcgaagcgta tggcaccttg cgacgcgcgg tggcgaagga
     2461 tctgccgtcg ccctgatgtt tccaaggatt atgaggatct gatgctcgtc ccatcaggag
     2521 tggagtcttt tcgaaagacc ctctatcttc ggccgcgtcg tctgggacgg aaaaccgtgg
     2581 ccgaggattc tatgtcggaa cattgccacc acttgcattc gatccgggat gtcacgccca
     2641 gggcgcgcgg ctgcgaggaa tgtctcaaga cgggagattg gtgggttcat ctgcggattt
     2701 gccgtacctg cgggcacgtc ggatgttgcg acgattcacc caacaagcat gccaccgcgc
     2761 atttccatgc gacaagccat ccgatcatcg aaggctatga tccaccggaa ggatggggct
     2821 ggtgttacgt cgaggaagtc ttcgtggacc ttcctgatca aacgccgcag ctcggcccga
     2881 tcccgcgtta catttgatga gcccctcgcg cccttccaaa tctgtttcgg gctatgatgg
     2941 cgcgatgccg acggacccct atgaccggcc ggcgcaaacc ttcccgaagc tgccgccgga
     3001 gatggtggca agaatcaaga aatacgggca gacggacagt ttcgacggtg gcgcatatct
     3061 ttacaatgtc ggcgatcgcg acgttgattt ctttctcatc cttgaaggtg gcgtcgatgt
     3121 tttggagagc gacggccacg gcggtcatgt tctcgtcgtc acacatgaag cgagcgaatt
     3181 caccggcgaa ctggatcatc taagcggccg agccgtgttc gtctgcgccc gcgcggcgcg
     3241 accgacgaag gtcgtgcgta tttcgtccgc atcgtttcgc aaattggcga gtgccgagcc
     3301 cgacattggc gagatcatcc ttcgcgcttt cattttgcgc cgcgttggcc tgatccagca
     3361 tgccgaaggc ggcacgatcc tggtgggcag tcagcgttta agcgacacgc tgcgtattca
     3421 aagcttcctc actcgcaacg cctgcccgca tcgttttcta gatacggatg tggaccccga
     3481 tgctgctatt gcattgaaat attttcacct cgagggcaac gctcagccgg tcgtgatcat
     3541 caatgcgcgt gacgtcttgc agaagccgac gaacagcgaa ctcgccgatg cgctgggcat
     3601 caccgaacat ctcgacccag atcgcatcta tgatgtcgtc gtggtcggcg ccgggccagc
     3661 cgggctggcc gctgcggtct atgcggcgtc cgaaggtctc gacacggttg tcatcgaatc
     3721 gatggcgccg ggaggacaag ccggcagcag ttcgcgaatc gagaattatc tcggctttcc
     3781 gacagggatt tcagggcagg cgctgtccgg acgcgcgcag gtgcaggcgc agaagtttgg
     3841 cgctcggctc gccatcgccc gcgccgcgat aggtctggat tgcggctttc cctttcgcgt
     3901 tcacatggaa ggtagaactg cggtcacgac gcgggccgtc gtcgtggcct ccggcgcgcg
     3961 ttaccggcgg ctcgatgttg aagacttggc gcggttcgaa ggtcagggcg tgcattatgc
     4021 ggcgacgccg atcgagggag gcctttgcgc cggcgccgag atcgttgtcg tgggcggtgg
     4081 gaattcggcc ggtcaggccg ccgtctatct ttcgcgcaaa gcgagacacg ttcatatctt
     4141 ggtgcgcggc gacgggctcg cggcaacgat gtcggattat ctcgtcaggc gcatcgagga
     4201 atccgcgcag atcacgcttc acacgcgatg cgacattacg gctctcatcg gcgacaaata
     4261 cctcgaagcc gtacgatggc gcgataaaga tggcgccgaa acgcaagttt caacatcgaa
     4321 tctttttctc atgatcggcg ccgtgccgaa taccgattgg ctcaacggct gcgttgatct
     4381 tgatccgaag gggttcgtgg aaaccgggca tataaaagcc ggcgaggcgc tgacgacgcc
     4441 ttacgtaacg agcgtcccag gcatttttgc cgtcggcgac gtgcgcgcca attcggtcaa
     4501 gcgggtggca tccggcgtcg gagaaggctc cgtcgtggtg cacgcgatcc atcgctggct
     4561 cgccaaacag gccgctaccg atccaagcaa ttagtcggct ctgccgcgga taagcacggc
     4621 tgcaatgagg tcgatcgcca gcgcagccaa cagcgctatt gtgccaatca cgaacagcaa
     4681 tctgtaattg ccgccgcttt gcgcaaagat gaaggaaagc ccgtaggccg ccgtcgcctg
     4741 cagcgtcgcg aaactgacgg tcgccgcgct ccacgcgccc ttttgctcgg cggggtgatg
     4801 cgcgagcaat tcatgcaccc gccccagcac caacggcacg atgccgggcg tgaaggcgcc
     4861 gacgacgaca ctcgatatga ccaatgacac cgacccgagg ccaagcgccg gtaacacgac
     4921 ggccgcggct tgcagcaagt aagcaagacg taaggcaggg ccgaagccgg cgcggtccgc
     4981 aagatgccag gtcagcaacg gtccgatagt cgcgcccaat ccgaacagca cccaatattg
     5041 cgcaccggtt tgcagaccct ggccgaggcc acgagcgata taatcgacaa ggaagatcat
     5101 gtgcggcaca agccccgccg cgttcagcgc atattccacg taaagcgcct tgagcgcgcc
     5161 gcggtgatgc ggccgctgaa gtggctcgat attggcggcc ttcggcgccc cggctggcca
     5221 gccgctccac gccacgacgg tcaacgcgag ggagagcaat ccgagcccga gccacgtttc
     5281 agtcagactt tggcggagca aaaatggaac gagcgtgccg gaagcgatca caccgatgcc
     5341 gacgcccatg aaaataattc cgccagcgag accccgccgc ccggcaggga cgtgcgcaag
     5401 gaccgttggc gccgccagca ccatcaacac gccaccggaa agacccgcga caaaccgcca
     5461 ggcgaagaac cagagaaacg agagcgggaa ggcgcaggcg aagaacgccg ccgtcgccaa
     5521 gatcatcatc agccgtagcg cgatcgcaac tggcagccgc accgcgagtt gccgaccgag
     5581 cagcgcgccg ccgagatagc cggcgagatt ggcggccccg agataagccg cggccgatgg
     5641 cgcaaaccag tgcgcattga tgatcgccgg cagcaatggc gcataggcga aacgcgcaag
     5701 gccaatgccc acaagactcg tgcagaaggc cgagagagta gcgcgccaga cgctgaggtc
     5761 ggagatcgcc gttccatgcg gcgtttgatg cctgacgatt ttatccatgt cgcccttcgt
     5821 gccgaattca ggaggcgcat tgcgcacctc ctgaacggtc gatgagcctt acttggcggt
     5881 ctctgccagc ttctcaagca gtacttttgc cgcaggcccc gacgaggcag ggttttgacc
     5941 tgtggtcagc agaccatcgc tgacaacatg cacgccccag tctgcggtct tggagaaaat
     6001 accgccttcc gccttcaact cgtcttcgac aaggaaaggc acgacctttg tcaggccaac
     6061 ggcttcttcc tcagtatcgg tgaagccagt gacctgcttg ccttcaacca gtggccggcc
     6121 ctctggtgtc ttcacatgcc gcagcgcgcc tggcgcatgg cagacaagcg cgacaggctt
     6181 gccagccgca agtaatgatt caatcagctt gatggaattt ggatcctcgg cgaggtccca
     6241 catcgggcca tggccgccgg gataaaacac ggtgtcgaaa tcttcggatt tgacgctgtc
     6301 gagccgcagc gtcgtggcga gctgcgcctt ggcatcggca tccgcctcaa agcgccgcgt
     6361 aagatccgtt tgaaaattcg gttcattgct cttggggtca agcggcggct ggccgccttt
     6421 tggcgaggcg agggtgatgt cggcgccggc gtccttgaaa acgtagtaag gcgcggcaag
     6481 ctcctcaagc cagaagccgg tctttcgtcc cgtgttgccg agcttgtcgt gggaggtcag
     6541 gaccatcaga attttcatgg aaatctcctt ggtcgatcaa ttagcggagg cgtcaataaa
     6601 gcctatgtca atgaagcttg taaggcgatg cgcttcaccg atcgctgcca tatatggatg
     6661 atgcacccac gttgccctta aacaactggg aaggtggggt ctccgttaaa attcaacacg
     6721 cctcgccaat acatctggaa ggatcgatcg atggctgact tgttttcgcc gggcaaaatc
     6781 ggcgctattc ccgtgtccaa ccgcgttgcg atggcgccgc tcacccgcgc tcgcgccgac
     6841 atggacggcg tccacacgcc gcttgcgatc gaatattacc gtcagcgcgc gtccgccggc
     6901 ctcatcatca gcgaggcaac gaatatctcc cgacagggca ggggctacgc gttcacgccc
     6961 gggatttaca cggatgcgca tgtgcgcgcg tggcggcccg tgaccgaggc cgtccacgaa
     7021 gccggtggcc acatcgtttt gcaactctgg catgttgggc gcatttccca cgacagcctg
     7081 caggaaggcg gccagccgcc ggttgcgcca tcggcaatcc aggccggagg attgacttat
     7141 atcgaaacgg gcttcgagcg accgtccatg ccgcgggcgc tccgcactga tgaaatcccc
     7201 ggcatcatcg acgattatcg caacgccgcg cgcaaagcga aggacgccgg atttgacggt
     7261 atcgaagttc attcggccaa ttgctatctg cttgaagagt tcatccgcga catcaccaat
     7321 aaacgcgatg acgcttatgg cggatcgatc gaaaatcgga cacggctgac gtttgaagtc
     7381 gcgcgagccg ttgcggaaat ctggggtccc gagcgcgtcg gcgtgcgcct gtcgccactg
     7441 acgaaatccg cgggcgatac gccgctcgat agcgatccgc agacgaccta cgggtttctg
     7501 gccgagagac ttggcacgct taaccttgcc tatctgcatt gcatcgaagg ccagacgcgt
     7561 ggcgacaatg cgaaagctga atttgatttc caggctttgc atcgggcttt tggcggtgcc
     7621 tatatcgcca acaacggcta tgatcgcgaa ctggccatcg aggccatcgc gacccaccac
     7681 gcagacatgg tagcattcgg gcggcccttc atcggaaatc ccgatctggt cgagcgtctg
     7741 cagcgcgacg agccgctcgc gacggccccg caggagacct tttacggcgt tggcgcaaaa
     7801 ggctacatag atttcccgac attcgcggcg gcgtcaacgt cgtaaagcgg cgcggtctgc
     7861 gaccttgtgg agggcatgcc gtggctgaaa aatcctcccg ggtccagatc aagagagtct
     7921 atgagcccgc ctcgccggcc gacggcgctc gcgtgctcgt cgatcgtatt tggccgcgtg
     7981 gattgagcaa ggcacatgcc gacgtcaaat tatggctgaa ggacatcgcg ccgagcaccg
     8041 acttacgcaa atggtttggt cacgagccgg agcgctggcc ggaatttcgg acgcgttact
     8101 gggcagaact ctcgaaaagc gaagaagccg tgacccaatt gctcgacgtc gttaaacacg
     8161 gccacacgac acttgtttat gcagcgcacg atgaattgca caacaatgcc ctcgcgcttc
     8221 tcgaatatct cgctgcgcat cggcattgag agcagcgcgc cgcaggccct aacacctccg
     8281 acgcgactgg ctgctcgata acaaaccggt caacggcgtt ccgttgggtg tgaccggagc
     8341 agtcggtggg cttatgctgc ccgcatcatg ctgccagccg ctgcaaggta tgacttcatc
     8401 gcgtttaggc cgattctgaa agtaataaat cgcggcgact atgaacacgc caacgaagat
     8461 caaggcgata attttcgtcc ccttggtcaa acgctgacca ctctcgataa tggaactcat
     8521 ccaaaacctc gggcaggaac acaccgctcc gcagccttcg gtctatatcc ggaaaatcgg
     8581 cgagcgggcg ttttatgcgt tcagtgatgt ggatgtgccg gtatccgggc cgacgccagc
     8641 cgggctcccc ggccaaacct gattgcggcc attgcgcttg gcggcataaa ggcatgaatc
     8701 ggcattagcg acgattccat tggcggtgag atcgaacgcc ggctggccgg aggcgacgcc
     8761 gaaactcgct gtgacatagg ctgcagagtc gagcgccggg tggggaatgc cggcgctttc
     8821 gatcgcgcga cgcatccgct cggcgacaac aagcgctttc gccattggtg tctgtggaag
     8881 gacaataatg aactcttcgc cgccgaagcg gaacgcgtga tcggcttggc cgcgcaattc
     8941 cgcctggatg atgccggcta cgcgtttgag gcaaagatcg ccagcctgat gcccggcgga
     9001 atcgttgaag agtttgaaat gatcgatgtc gagcaagaca atcgagagct cgaccgcgaa
     9061 gtcgaattgc tcgcaggcgg cgagagtttc ttcaagcgcg cgccggttgc cgaggccggt
     9121 cagcgagtcg cgacgggtca ttttgtcgag ttctgaattt tgcgccaggc cgcgctgcga
     9181 cagcaggtag tgcaggcgca gatcgcgctc catcgtatag gaggcgatga gcgcgaaaat
     9241 caccgctgcg ctgaatacca tgttgatcgc gacctgctgt cccgttgcat aatgataaaa
     9301 atgtgcgaca gcgagaacat gccctacgat gctggcgagg cacgtcggcg ctaggaacga
     9361 aaaccgcgtt ctttgcacga ctgagagaaa aagaacgacg aggatcattg attcatacag
     9421 ggtcgcttgc ggtgcggtgc cgctcgcggc catgaggaag atgatcgtcg cgacggagat
     9481 ggcaccgccg ccaacacaga cgaggctttc gcggacaaag gcgcgaggac gcgtgtgcag
     9541 caccgccgta agaattaggg ccacgggcgt gaaaaacgcc gcacgaaccc aaaaagccgt
     9601 ccagaaaagc tttggcgtcg cccaataatc cgcgatcgcg aataggtcat agacgacgat
     9661 gccgatatag gcccggacga taaggtcgcg gcatctttcc gcacccgtgt cgcgctcaaa
     9721 ggccgcctcc aactttccgg gaaatcgcaa cagccagttt ctggactgta tcgcggcgtc
     9781 aatagaggcg aggtcagcgc ttgttgacga tgaaatatgt gcgccgttat cggccatgca
     9841 gacgcctgcg tcctacccgc cttgagaggt ggcgaaatta ttagccaggg aggcttaaca
     9901 atcgctctcg gtggctccta catctctgct tatggccgat tttcctgggt ccaatacctc
     9961 acaatcgcgt tggcgcgtgg ccggggcgtt gccttctctg tcctgtaaca aatccgcgga
    10021 attggcgtaa taatgctgga aagcgcggag cccaagccgc gcgagggagt gaatatcatg
    10081 gacgtcaaag actatcccgt cacgcattcc gaggctgaat ggcgcaagct gctgacgccg
    10141 gagcaatatc aggtcatgcg cggccacggg acggagcggc ccggctcctg cgcgctcaac
    10201 ttcgaaaagc gggctggaag cttcacctgc gctggatgcg accagccact gttcgtctcg
    10261 aagacgaagt ttgagagcgg cacgggctgg ccgagcttca acgatccggt gcccggctcg
    10321 gtcgagacca gcgtggacaa cagctacggc atggagcgcg tcgaagcgca ttgcagccgc
    10381 tgcggcagcc atctcgggca tgtcttcccc gacgggccgc cgccgacgca cctgcgctat
    10441 tgcatcaatg gcgtggcgat gaatttccac ccggaatagg ctgtcagggc atccccggac
    10501 tcgatccggg gatcgccccg accatcc
//
LOCUS       DASWOG010000519         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_31016,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000519 DASWOG010000000
VERSION     DASWOG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31016"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGB93_10705"
     CDS             <1..752
                     /locus_tag="VGB93_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HEY0146593.1"
                     /translation="GSGRHSDGRVVLAITGMKAEARIAAGAAVSVIASGGNRSILETA
                     LDNVFEANIGAVLSFGIAGGLAPGLPAGTALVARKIVSDDGEYECDRAWSQRLAAALG
                     GAPIVDMAGIDEAVATPADKRALHIATRAAAADMESHIAARFAAERNLPFAAFRVVAD
                     PAERHVPPAALAGIGKTGRVTVGPVLRSLARAPGQFPHLVKTALDANAAFMTLFRSRQ
                     MAAGGGFGFFDFSELVLDMPREDVIGGSLPV"
     gene            complement(639..1790)
                     /gene="hpnH"
                     /locus_tag="VGB93_10710"
     CDS             complement(639..1790)
                     /gene="hpnH"
                     /locus_tag="VGB93_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY0146594.1"
                     /translation="MGIPLLQALQIGGYVLGQKLKGNKRFPLVLMMEPLFRCNLACAG
                     CGKIDYPDPILNQRLPLDEALHSMEECGAPVVVIAGGEPLLHKELPKIVEGGLAQGRY
                     MTVCTNALLLEKQIDRFKPHRRFNWSIHLDGDQEMHDKSVCQKGVYDRAVSALKLAKS
                     KGFRVTINCTLFNDADPERVASFFDDMKELGIEGVTVSPGYAYERAPDQQHFLNRAKT
                     KQLFRDIFKRGNGGKKWPFFQSGLFLDFLAGNRTYECTPWGNPTRTVFGWQRPCYLLG
                     EGYTKTYAELMETTNWDSYGVGKYEKCADCMVHCGFEATSVNEAFSKPWEILKVAIGG
                     IRTDGAMAKDVSLDGQRPADYVFSRHVQNKLAEIKEAETATSSHLSAAE"
     gene            complement(1825..>2031)
                     /locus_tag="VGB93_10715"
     CDS             complement(1825..>2031)
                     /locus_tag="VGB93_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0146595.1"
                     /translation="ADRAELDLGLVRGANTIGLTAGASAPEVLVEGVIEALRGLGPLD
                     VALLPGVQENVEFRLPFEFLATSA"
BASE COUNT          390 a          621 c          604 g          416 t
ORIGIN      
        1 atggttccgg gcggcattca gacggccgcg tggttcttgc tatcaccggg atgaaggccg
       61 aggcgcgaat tgctgccggc gctgccgttt ccgtgatcgc cagcggcggc aacagaagca
      121 tcctcgaaac tgcgctcgac aatgtctttg aggccaatat cggcgcggtg ctgagtttcg
      181 gaattgccgg cggtctggcg ccaggcctac cggccggcac ggcactcgtt gcgcgcaaga
      241 tcgtttccga cgatggcgaa tatgaatgcg accgcgcctg gtcgcagcgg cttgccgcgg
      301 cgctcggcgg cgcaccgatc gttgacatgg ccgggatcga cgaggccgtg gcgacacctg
      361 ccgacaagcg cgccctgcat atcgccaccc gcgctgccgc cgccgatatg gaatcgcata
      421 tcgccgcacg cttcgcggcc gaacgtaacc tgccgttcgc agcgttccgc gtcgtcgccg
      481 atcccgccga gcggcacgtg ccgccagcgg cgcttgcggg cattggaaag accggccgcg
      541 tcactgtcgg gccggtgttg cgctctttgg cgcgggcgcc gggtcagttc ccgcatctgg
      601 tgaaaacggc gctcgatgcg aacgccgcgt tcatgacctt attccgcagc cgacagatgg
      661 ctgctggtgg cggtttcggc ttctttgatt tcagcgagct tgttctggac atgccgcgag
      721 aagacgtaat cggcgggtct ctgcccgtct aacgagacat ccttcgccat tgcgccgtcg
      781 gtgcggatac cgccgatggc gactttcaga atttcccacg gcttggaaaa ggcttcgttg
      841 acggacgtcg cttcgaagcc gcaatggacc atgcagtcgg cgcatttctc atatttgccg
      901 acgccatacg aatcccaatt ggttgtttcc atcagctcgg cataggtctt ggtgtaacct
      961 tcgcccagca aatagcacgg gcgctgccag ccgaagacag tccgcgtcgg gttgccccac
     1021 ggtgtgcatt cgtacgtgcg gttacccgcg agaaagtcga ggaacaggcc cgactggaag
     1081 aacggccatt tcttgccgcc attgccgcgc ttgaagatat cgcggaacaa ttgcttcgtc
     1141 ttcgcgcggt tgaggaagtg ctgctgatcg ggcgcgcgct cgtaagcata gcccggcgag
     1201 accgtgacgc cttcgatgcc gagttccttc atgtcatcga agaaactagc gacacgctcg
     1261 gggtcggcat cattgaagag cgtgcagttg atcgtcacgc ggaagccctt cgacttggcg
     1321 agcttcaatg ccgacacggc acgatcgtaa acaccttttt ggcagactga tttgtcatgc
     1381 atctcctggt cgccatcgag atgaatcgac cagttgaaac gccgatgcgg cttgaacctg
     1441 tcgatctgct tctccagcaa cagagcattg gtgcagacag tcatatatct gccctgcgca
     1501 agaccgccct cgacgatctt aggcaattcc ttgtgcagca gcggttcgcc gcccgcgatc
     1561 acgacgacag gcgcgccgca ttcttccatc gaatgcaggg cttcatcgag cggcagacgc
     1621 tggttcagga tcggatcggg atagtcgatc ttgccgcagc cggcgcaagc gagattgcag
     1681 cggaacaaag gttccatcat gagaacgagc gggaatcttt tgttgccctt gagtttctgg
     1741 cccaggacat aaccgccgat ttgcagcgcc tgcagtaacg gaatacccat ctaacgccgg
     1801 ctcctatatg agtggctcaa ttgtttaagc tgacgtcgca aggaattcaa aaggcagccg
     1861 gaattcgaca ttctcttgaa cgccgggcag aagcgcgacg tccagcggcc ccaaccctcg
     1921 caaagcttcg attacccctt cgaccagaac ctccggtgct gaagcgccag ctgtcaggcc
     1981 aattgtgttt gccccacgca caagaccaag gtcaagttcc gccctgtcgg c
//
LOCUS       DASWOG010000520         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_85312,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000520 DASWOG010000000
VERSION     DASWOG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85312"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            789..1148
                     /locus_tag="VGB93_10720"
     CDS             789..1148
                     /locus_tag="VGB93_10720"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HEY0146596.1"
                     /translation="MKQASLVHLSIDELVNLFRQNATEQDRVIFKEQVSKYEEVFRES
                     DTITAELQKRGPGALRALMRLYDDPNMQVRLQAARLTVGIAPAEAQGVLKNIARFGHV
                     PQSANARAKLRSLGVEW"
     gene            1282..2688
                     /locus_tag="VGB93_10725"
     CDS             1282..2688
                     /locus_tag="VGB93_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEY0146597.1"
                     /translation="MSRAAAHLRVTAAFLFATLLCTSARADEAPPVSPDKPAPLVTPS
                     RVSATVEFSIPVLARDVERDVPRRLATFDERIQCVHRRVMFFRVRANCDIEGYVERTG
                     PISLYGRGDAIIGSTPIFGTVSGQGANRFTRRIHGEAEAGVTVEAEARPMLRHDWSLD
                     LRLSDSFHWTEPPVLHVLGRDIQLARYVEPRIREQLARVRVRAAEAVRRLDLHGKAAT
                     VWRHAFEPVELADNPPVWLQLVPQSAAFAGVHADRRVLWGSLEIEGNATTTVGTAPAP
                     VAATPLPPLGRDVQAPGKFEVILPVHVGYDFLSQKFKDAVAAQLKGDYTLHDVKIYPS
                     NGKIIIGLKIAKKEDTAPDAGKWFYLSATPKVDPDTQTLQFPDLAALAAKAEAMAQAS
                     GNADLVDELRQAKILYQEAYQRLLTVANQRLTRPLKDGFRMEGHLDSAKLDHVSLLQD
                     GISIALRMSGNLKILYGL"
     gene            2688..>2884
                     /locus_tag="VGB93_10730"
     CDS             2688..>2884
                     /locus_tag="VGB93_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEY0146598.1"
                     /translation="MRIAYLLAAFVFFASPAAAQGRFYDAPRIDALGKPGTLVRSQAI
                     EGAPLNAAAYRVLYRSTSFDG"
BASE COUNT          562 a          837 c          885 g          600 t
ORIGIN      
        1 taggttgacc gattttctcg ccaatgcatt atgtaatttt accgttaaca taaaggtata
       61 attacctgaa cgccgctcca tcaaggaggg acactccatg tcatctgaaa aaaacatcct
      121 ggacggcgga gcgggtcgcg cggctcggct ttctggtcgg gcacggtttc ggcgccaaac
      181 gcatcgccga ggatccgttc atcgcctcga cgcccaacaa tgtgcatcgt caggcccagc
      241 gcttcggcct cgcctttcgc gacgcgatgg cgacggccct gcgtctgccg ccggaggccg
      301 ctgcccgcta cgacgccgcg gccgccaaac gcgggctgac ccgcgtgtca acggtaggaa
      361 gcgaggaatt tccagatgta aaagggcttg caacgcacct gagtctggat gtctctcgag
      421 cacggattgg gccaaccaaa cgggtggaat gcatcaacgg cgcgctcttt aaagacggag
      481 gaccgacagt cgagattact taggggattc ttccgcggcg ttcgatctgg gtgctattcc
      541 aaggctcgtt ctattcccta cgagagtctg aagcacgaag tgctcggacg ggtcaaaaaa
      601 ttcaacggtc gaaatatggt accggatttg cgatcgtctt cgagcttgat gggtggcatt
      661 gcggttacac ggttgattcg gaggcttcgg acatacacgc ggtgttttgc gaagcgctaa
      721 cgacaacata actagaccct gggtgtagga gggatctttt cgcttaaaga ttttggagtg
      781 ctcgagaaat gaagcaggca tcgctcgtgc acctttctat cgatgagctg gtcaatcttt
      841 tccggcaaaa tgcgactgaa caggatcgcg tcattttcaa agagcaagtc tctaagtacg
      901 aagaagtttt tagagagagc gataccatca ccgctgaact gcaaaaacgg gggcctggcg
      961 cactccgtgc tttgatgcga ctttatgacg acccgaacat gcaggttaga ttgcaagctg
     1021 ctcggttgac agtcgggatt gcgccagcgg aggctcaagg cgtgctcaaa aacattgcgc
     1081 gttttggtca cgtgccgcaa tcagccaatg cgcgcgccaa attacgaagt cttggagtcg
     1141 aatggtagtc gcgtgcgacg acggtcgatc aatctcgcca cggcgaagtg ctggggacgc
     1201 aggcatgaca atctccaatc ttgacggagg cgtcattaag ccggattaat ctgcaaagca
     1261 gtcaagtggc ggagactggc gatgagtaga gcggcggcgc atcttcgggt tacggcggcg
     1321 tttctctttg cgacgctttt atgtacgtcc gcccgcgcgg acgaggcgcc gccggtcagt
     1381 cctgacaagc cggcgccact cgttacgccg tcgcgcgtat cggcgaccgt tgaattcagc
     1441 attcccgtgc tggcccgcga tgtcgagcgc gatgtgccgc ggcggctcgc gactttcgat
     1501 gagcgcatcc agtgcgtgca tcgccgcgtg atgttctttc gtgtccgcgc gaattgcgac
     1561 atcgagggct atgtcgagcg caccggcccc atctcgctct atgggcgtgg cgatgcgatc
     1621 atcgggtcga cgccgatctt cggtacggtg agcgggcagg gcgcgaaccg gttcacgcgg
     1681 cgcatccatg gcgaggccga ggcgggcgtg acggtcgagg cggaagcgcg gccgatgctg
     1741 cgccacgatt ggtcgcttga tcttcgcctg agcgacagtt tccattggac cgagccgccc
     1801 gtgcttcacg tgctcggccg cgatatccag ctcgcgcgtt atgtagagcc acgtatccgc
     1861 gaacagcttg cacgggtgag agtccgcgcc gccgaggcgg tgcgccggct cgatctgcat
     1921 ggcaaggcgg cgactgtctg gcgtcatgct ttcgaacccg tcgagcttgc cgacaatccg
     1981 ccggtctggc tgcagctcgt cccgcagagc gccgctttcg ccggcgtgca cgccgatcgc
     2041 cgcgtgctgt ggggatcgct tgagattgag ggcaatgcca caaccaccgt cggcactgcg
     2101 ccggcgccgg tcgccgccac gcctttgccg ccgctcggcc gcgatgtgca agcgccgggc
     2161 aagttcgagg tcatcctgcc ggtgcatgtc ggctacgatt tcctgagtca gaaattcaaa
     2221 gatgcggtgg cggcccaact aaagggcgat tacacgctgc acgacgtcaa gatctatccg
     2281 tcgaacggca agatcatcat tggcttgaaa atcgcgaaga aggaggacac ggcgccggat
     2341 gccggcaagt ggttctatct ttccgcgacg ccgaaggtcg atcccgatac gcagaccttg
     2401 cagtttcccg atttggccgc cctcgccgcg aaggccgagg cgatggcgca ggcgagcggc
     2461 aatgccgacc tcgtcgatga gcttcggcaa gcgaaaattc tttatcaaga ggcctatcaa
     2521 cgcctgctca ctgtcgcgaa tcagcgtctg acgcggcctt tgaaggatgg cttccgcatg
     2581 gaaggccatc tcgactccgc caagctcgat cacgtctcct tattgcagga cgggatcagc
     2641 attgcgttgc gcatgagcgg caatctcaaa attctctacg gcctctgatg cgcatcgcat
     2701 atttgctggc ggcgttcgtc ttcttcgctt cgccggccgc ggcgcagggc cgcttctatg
     2761 atgcgccgcg cattgatgcg ctcggcaaac ccggcacgct cgtccgctcc caggcgattg
     2821 aaggtgcgcc gctcaacgcg gctgcctatc gcgtgcttta tcgttcgacg agtttcgacg
     2881 gcaa
//
LOCUS       DASWOG010000521         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_213775,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000521 DASWOG010000000
VERSION     DASWOG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213775"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGB93_10735"
     CDS             complement(<1..593)
                     /locus_tag="VGB93_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0146599.1"
                     /translation="MRRSLIFVFAAALALFAPVRSIADDITPLGIGLEDIDYPYPVHF
                     LDLTIEGQRLRMAYMDVSAAHPNGQSIVLLHGKNFDGEYWAQTIRMLADHGYRVIVPD
                     QIGFGKSSKPDIDYHFDLLAANTKALLDHLGVAHATIVGHSFGGMLAVYFARDYPQTT
                     TRLVLENPIGLEDYRSAIKPPTLDSLFNAEMAQTAAS"
     gene            complement(756..>1621)
                     /locus_tag="VGB93_10740"
     CDS             complement(756..>1621)
                     /locus_tag="VGB93_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEY0146600.1"
                     /translation="WPAYMTAERIAKAKNLKIIITAGIGSDHTDLEAANKHKITVAEV
                     TYCNSISVAEHVVMMILGLVRNYIPSHDWVRKGGWNIADCVERAYDLEGMHVGTVAAG
                     RIGTAVLRRLKPFDVKLHYTDRHRLPAEVEKELGLTFHENREDLFKVCDVITLNTPLH
                     PETEHMINDRTLALMKRGTYLVNTARGKLADRDAIVRALENGQLAGYAGDVWYPQPAP
                     KDHPWRTMPHEGMTPHTSGTTLSAQARYAAGTREILENFFAGKPIRDEYLIVQGGHLA
                     GVGAHSYSVAK"
BASE COUNT          315 a          454 c          528 g          324 t
ORIGIN      
        1 taagaggcgg ccgtttgcgc catttcggcg ttgaacagcg agtcgagcgt cggcggctta
       61 atggcgctgc gataatcttc aaggccgatc gggttttcca gcacgagccg cgtggtggtt
      121 tgcggataat cgcgggcgaa ataaaccgcg agcatgccgc cgaacgaatg accgacgatt
      181 gtggcgtggg cgacgccgag atggtcgagc agggccttcg tgttcgcggc cagtagatcg
      241 aaatgataat cgatatccgg cttcgaggat ttgccgaacc cgatctggtc cgggacgatg
      301 acgcgatagc cgtgatcggc gagcatgcgg atcgtttgcg cccaatattc gccgtcgaaa
      361 ttcttgccgt gcaagagcac gattgattgg ccgttcgggt gcgcggctga cacgtccata
      421 taggccatgc gcaggcgttg accctcgatc gtcagatcga gaaaatggac cgggtaggga
      481 tagtcgatgt cttcaaggcc gatgccgagt ggcgtgatat cgtcagcgat agaacgtacc
      541 ggcgcaaaaa gcgcgagcgc cgcggcaaac acgaaaatca ggctgcgtcg catcgccggg
      601 ccctccagcc gcaagtcggc tcgggaatag gctgcgctcg gaaaatatca cgtcacattc
      661 acgcgaggta ggcccggcac gtcgcgccgg accgaaaagt gtaagcggtt ttcggacgat
      721 ccggcgcgat gacgaatcgc gccgggcccg tcatatcact tggcgacgct gtacgaatgc
      781 gcgcccacac cggcgagatg gccgccctgg acgatcagat attcgtcgcg gatcggcttg
      841 ccggcgaaga agttctcgag gatttcgcgc gtgccggcgg catagcgggc ctgcgccgaa
      901 agcgtcgtgc cggatgtgtg gggggtcatg ccttcatgcg gcatcgtccg ccacggatga
      961 tccttcggcg ccggctgtgg ataccagacg tcgccggcat aacccgcgag ctggccattt
     1021 tcgagcgcgc gcacgatggc gtcacgatcg gcaagtttgc cgcgggcggt gttcacgaga
     1081 taagtgccgc gtttcatcaa cgcgagcgtg cgatcgttga tcatatgctc ggtttcagga
     1141 tgcagcggtg tgttcagcgt gatcacatcg cagaccttga acagatcttc gcggttttcg
     1201 tggaatgtca ggccgagctc cttttcgacc tcggccggca ggcgatgccg gtcggtgtag
     1261 tgcagcttca cgtcaaaggg cttcagccgg cgcaacaccg cggtgccgat gcggccagcc
     1321 gcaacggtgc cgacatgcat gccttcgaga tcgtaagcgc gctcgacgca atcggcgata
     1381 ttccagccgc ctttgcgtac ccaatcgtgt gacgggatgt aattacgcac gagaccgagg
     1441 atcatcatca ccacatgctc agcgacgctg atgctgttac aataggtgac ctcggcgacg
     1501 gtgatcttgt gcttgttggc ggcttcgaga tcggtatggt ccgaaccgat gccggccgtg
     1561 atgatgatct tgaggttctt cgccttggcg atgcgctcgg ccgtcatata ggccggccag
     1621 a
//
LOCUS       DASWOG010000522         5911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_48700,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000522 DASWOG010000000
VERSION     DASWOG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48700"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGB93_10745"
     CDS             <1..624
                     /locus_tag="VGB93_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0146601.1"
                     /translation="AEIPLIVAITEGIPVLDMVRVKRALCGSKSRLIGPNCPGVVTAG
                     ESKIGIMPANIFLPGSVGIVSRSGTLTYEAVFQTSREGLGQTTAVGIGGDPVKGTEFI
                     DVLELFLADDKTQSIVMIGEIGGGAEEDAAQFLRDERKKGRAKPIVGFIAGRTAPKGR
                     RMGHAGAIISGGKGGAEEKIAAMEAAGIVVSPSPARLGKTLADVLNR"
     gene            748..3699
                     /locus_tag="VGB93_10750"
     CDS             748..3699
                     /locus_tag="VGB93_10750"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0146602.1"
                     /translation="MPRSTSNHTFALTSFLDGSNAAYAEQLYENYTRDPNSVDPQWRS
                     FFAELKDDPILVEKSVQGPAWKAEVAPSPRDETLAALDGNWDAIESALGAKLKDVHKD
                     GSHSDAELRQATRDSIRALMMIRAYRMRGHLEADLDPLGLQAKGEQELLHPSNFGFTE
                     ADYTRQIFIDGVMGLKFATIPQMLTILRRTYCGTIGYEFMHISDPAEKSWLQARIEGP
                     NKEITFTREGKRAILNKLIEAEGFEKFIDLKFTGTKRFGLDGGESMIPALEQVIKRGG
                     ALGVKEIIFGMAHRGRLNVLSQVMGKPHRAIFHEFKGGSFTPSEVEGSGDVKYHLGAS
                     SDRDFDGNHVHLSLTANPSHLEIVDPVVLGKVRAKQDQLHDYVERSKIMPLLIHGDAA
                     FAGQGVIAECFGLSGLKGHRTGGSIHFIINNQIGFTTYPRYSRSSPYPSDTAKMIEAP
                     IFHVNGDDPEAVVHVVKVAVEFRQMFHKPVVIDMFCYRRFGHNEGDEPSFTQPLMYER
                     IRAHPTTLQIYTKRLLDEGVVTEADVDEMKANWRKRLDEEFDAGQVEYKSNKADWLDG
                     RWAMLRPADQVDEARRGKTGVAIAKLRELGVGLCAVPDGFHLHKTIQRFLDTRKHAID
                     SGQGIDWATGEALAFASLLDEGYPVRLSGQDSERGTFSQRHSVLIDQQSEARYVPLNH
                     LRHGQARYEVINSMLSEEAVLGFEYGYSLAEPNALTVWEAQFGDFANGAQVVFDQFIS
                     SGERKWLRMSGLVCLLPHGYEGQGPEHSSARLERYLQMCAEDNMQVANCSTPSNYFHI
                     LRRQLKRDFRKPLILMTPKSLLRHKRAVSAIEDFDADKTFHRLLYDDAEMHEGGEIKL
                     VRDDKIRRVVLCSGKVYYDLLDERAKRGIDDVYLLRVEQLYPFPLKALIKKLSLYKKA
                     DIVWCQEEPKNMGAWSFVEPYLEWVLGQTGGKVSRPVYVGRPASAATATGLMPKHLAQ
                     LKAFVDEALAA"
     gene            3709..4932
                     /gene="odhB"
                     /locus_tag="VGB93_10755"
     CDS             3709..4932
                     /gene="odhB"
                     /locus_tag="VGB93_10755"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY0146603.1"
                     /translation="MTIEIRVPALGESVSEATIGRWFKKAGETVKADEPLVELETDKV
                     TLEVNAPSAGVLSEIAAESGTVVKPDALLGAIAEGAGAPAAPAASPKPAAPAASAPQA
                     AAPVAAPSMPPSPAAAKIAADNSLDPAAISGSGKRGQVLKGDVLSALIEAPTAPLVPE
                     PPLPQARVPVPVEDAPREERVRMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMAL
                     RTRTKDAFEKKHAIKLGFMGFFAKACVAALKEIPSINAEVDGADLIYKNYYHLGIAVG
                     TDKGLVVPVVRDCDRLSLAEIERNIADLGRRARGGSLKIEDMQGGTFTITNGGVYGSL
                     MSTPILNAPQSAILGMHKIEDRPVAIGGKVEIRPMMYLALTYDHRIVDGKEAVTFLVR
                     VKEALEDPARLLLDI"
     gene            4991..>5911
                     /locus_tag="VGB93_10760"
     CDS             4991..>5911
                     /locus_tag="VGB93_10760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0146604.1"
                     /translation="MYDLIVIGTGPGGYVCAIRAAQLGLKVAVVEKNKTFGGTCLNIG
                     CIPSKALLAASEKFAEADHALAGFGVIVDKPRLDLKGMMKHKDDTVAANVNGVAFLFK
                     KNKIDSFIGVGEIKKAGVVEVTADDGARQSLVTKNIVIATGSDVAPLPGLEIDEKQVV
                     SSTGALSLGKVPGKIIVVGAGVIGLELGSVWRRLGAEVLVVEFLDHILGPMDGEVGKQ
                     FQRMLEKQGFEFLLGHKVNKADKTGSGLKVTIAPANGGEEIIRDADIMLVAIGRRPFI
                     AGLGLEAAGVATERGRVVIDEHFATNVPGIY"
BASE COUNT         1211 a         1776 c         1820 g         1104 t
ORIGIN      
        1 gccgaaatcc cgttgatcgt cgcgatcacc gagggcattc cggtgctcga tatggtgcgg
       61 gtgaagcggg cgctttgtgg ctcgaaatcg cgtttgatcg ggcccaattg ccccggagtg
      121 gtcacggccg gcgagagcaa gatcggcatt atgccggcca acatctttct gcccggcagc
      181 gtcggcattg tttcgcgctc aggcacgctt acctacgagg cagtgttcca gacgagccgc
      241 gaaggccttg gccagacgac cgctgtcggc atcggcggcg atccggtgaa ggggacggag
      301 ttcatcgatg tgcttgaatt gttcctcgcc gacgataaga cgcaatcgat cgtcatgatt
      361 ggcgagatcg gcggcggtgc ggaagaagat gcggcgcaat tcctgcgcga cgagcgcaag
      421 aaaggccgtg caaagccaat cgtcggcttc atcgccgggc ggaccgcccc aaagggccgg
      481 cgcatgggcc acgcgggcgc gatcatttcc ggcggcaaag gtggcgccga agaaaaaatc
      541 gctgccatgg aggcagcagg aatcgtcgtt tcgccctcac cggcccgact cggcaagact
      601 ttggcggacg tgttgaaccg atagtttcaa gcctcggtac ggatcggaat cgccgccgtc
      661 cgagcaagat ttatgagagc gaaagctcgg ggaaagtata tggcacggca aggtagcaat
      721 gggcagaacg gctccggggg tgcagcgttg ccgcgctcca cgagcaacca cacctttgcc
      781 ctcacctcct ttctggacgg cagcaatgcc gcctatgccg aacagcttta cgaaaattac
      841 acccgcgacc cgaactcggt cgatccgcaa tggcggagct tctttgccga attgaaggac
      901 gacccgatcc tcgttgagaa aagcgtgcag gggccggcct ggaaggcaga agttgcgccg
      961 tcaccgcgtg acgagacgct ggccgcgctc gacggaaact gggacgccat cgaatccgcc
     1021 ctcggcgcga agctcaagga cgtccacaag gacggcagtc acagcgatgc ggaactgcgc
     1081 caggcgacgc gcgattcgat ccgcgccctc atgatgatcc gcgcctatcg tatgcgcggc
     1141 catctcgaag cagacctcga tccgctcggc ttgcaggcga agggcgagca ggaactcctg
     1201 catccgtcga acttcggttt caccgaagcg gattacaccc ggcagatttt catcgacggt
     1261 gtgatggggc tcaaattcgc gaccattccg cagatgctga cgatcctgcg ccggacttat
     1321 tgcggcacga tcggctacga attcatgcac atctccgacc ctgcggaaaa atcctggctg
     1381 caagcgcgca tcgaagggcc gaacaaggag atcaccttca cgcgcgaggg caagcgcgcc
     1441 atcctcaaca agctgatcga ggccgaagga ttcgagaaat ttatcgatct gaagttcact
     1501 ggcaccaagc ggttcggtct cgacggcggc gaatcgatga tcccggcgct tgaacaggtc
     1561 atcaagcgcg gcggcgcgct cggcgtgaaa gagatcatct tcggcatggc ccaccgcggc
     1621 cgcctcaacg tcctttcaca ggtgatgggc aagccgcacc gcgcgatctt ccacgaattc
     1681 aagggcggct cgttcacgcc ctcagaggtc gagggctctg gcgacgtcaa atatcatttg
     1741 ggcgcgtcct cggatcgcga cttcgatggc aaccatgttc atctgtcgct gactgccaat
     1801 ccgtcgcatc tcgaaatcgt cgatccggtc gtgctcggca aggtgcgcgc caagcaggat
     1861 cagctgcacg attatgtcga gcgcagtaag atcatgccac ttttgattca tggcgatgcg
     1921 gcgttcgccg gccagggtgt gattgccgaa tgtttcggcc tctccggcct gaagggccat
     1981 cgcaccggcg gctcgatcca tttcatcatc aacaaccaga tcggcttcac gacctatccg
     2041 cgttattcgc gctcctcgcc gtatccatcg gacacggcga agatgatcga ggcaccgatc
     2101 ttccatgtga atggcgacga tccggaagcg gtcgtccatg tcgtaaaagt cgccgtcgaa
     2161 ttccggcaga tgttccataa gcctgtcgtg atcgacatgt tctgctatcg ccgcttcggt
     2221 cacaacgaag gtgatgagcc gtcatttacg cagccgctga tgtacgaacg catccgcgcg
     2281 catccgacga cattgcagat ctacaccaaa cggctcctcg acgaaggcgt cgtcaccgaa
     2341 gccgatgtcg atgagatgaa ggccaattgg cgcaaacggc tcgatgaaga attcgatgcc
     2401 ggccaggtgg agtataaatc caacaaggcc gactggctgg acggacgctg ggcgatgttg
     2461 cgccccgccg atcaggttga tgaggcgcgc cgcggcaaga ctggcgtcgc cattgccaag
     2521 ctgcgtgaac tcggggtcgg cctctgtgcc gtgcccgacg gctttcatct ccacaagacg
     2581 atccaacgct tcctcgacac gcgcaaacac gcgatcgact cgggccaagg catcgactgg
     2641 gcgacgggcg aggctctggc cttcgcgagc ctgctcgacg agggttatcc ggttcgcctc
     2701 tccggtcagg acagcgagcg cggcacgttc tcgcagcgac acagcgtgct gatcgatcag
     2761 cagagcgagg cgcgctatgt gccgctgaac catctgcgcc acggtcaggc gcgctacgaa
     2821 gtcatcaact caatgctgtc ggaagaagcc gtgctcggct tcgaatacgg ctattccctc
     2881 gccgaaccga atgccttgac cgtttgggag gcgcaattcg gcgattttgc caatggcgcg
     2941 caggtcgtct tcgatcaatt tatctcgtcg ggcgagcgca aatggctgcg catgtccggc
     3001 ctcgtctgcc tgttgccgca tggctacgag ggccagggac cggaacattc atccgcgcgg
     3061 cttgaacgct atctgcaaat gtgtgcggag gacaatatgc aggtcgccaa ttgctcgacg
     3121 ccgtcgaatt atttccacat cctgcggcgg caattgaagc gcgacttccg caagcctctg
     3181 atcctgatga cgccgaaatc gctgctgcgc cacaagcgtg ccgtctcggc gatcgaggat
     3241 ttcgatgccg acaagacctt ccaccgcctg ctctatgacg atgctgagat gcacgagggt
     3301 ggtgagatca agcttgtccg cgacgacaag atccgccgcg tcgttctctg ttcgggcaag
     3361 gtttattacg atctgctgga cgaacgcgcc aaacgcggca tcgacgacgt ttatctcttg
     3421 cgcgtcgagc agctttatcc cttcccgctt aaggcactga tcaagaagct gtcgctttat
     3481 aagaaagctg acattgtctg gtgtcaggaa gagccgaaga atatgggcgc ctggagcttt
     3541 gtcgaaccct atctcgaatg ggtgctcggc cagaccggcg gcaaagtaag tcggcctgtc
     3601 tatgtcggtc gtccggcttc ggcggcgacg gcgaccggcc tgatgccgaa acatctggcg
     3661 caattgaaag cattcgttga cgaagcgctt gccgcttgag ggatgaaaat gacgattgag
     3721 attcgcgtgc cggctttagg ggaatcggtc agcgaggcca cgattggccg ctggttcaag
     3781 aaagccggcg agacggtgaa agccgacgag ccattggtcg aacttgagac cgacaaggtg
     3841 acgctggaag taaacgcgcc gagcgccggc gtgctcagcg agatcgcggc cgaaagcggc
     3901 acggtggtga agcccgacgc tttgctcggc gccattgctg aaggtgcggg ggcgccagcg
     3961 gctcccgccg cttcgccgaa accagcggcc cccgccgcat ctgcgccgca agcagcagcg
     4021 cccgtcgcgg cgccatccat gccaccgtcg cctgctgcag cgaaaatcgc cgccgacaat
     4081 agtctcgatc ccgcagcgat ctccggctcc ggcaagcgcg gtcaagtgct caaaggcgac
     4141 gtgctctcgg cgctgatcga agcgccgacg gcgccgctcg tgcccgagcc gccattgccg
     4201 caggcacgcg tccccgttcc ggtggaggat gcgccgcgcg aagagcgcgt gcggatgacc
     4261 aagctgcgtc agaccatcgc gcgccgcctg aaggacgcgc agaacaccgc cgcgatgctg
     4321 acgaccttca acgaggtcga catgagtgcc gtcatggcgt tgcgcacccg caccaaggac
     4381 gcgttcgaga agaaacacgc gatcaagctg ggcttcatgg gctttttcgc caaggcctgc
     4441 gtcgcggcat tgaaggagat tccttcgatc aacgccgagg tcgacggcgc cgacctcatc
     4501 tacaagaact attaccacct cggcatcgcc gtcggcacgg acaagggtct tgtcgttccg
     4561 gtcgtgcgcg attgcgatcg gctgagcctc gccgaaatcg agcgcaacat tgccgatctc
     4621 ggacggcgtg cccgcggtgg gtcgctcaag atcgaggaca tgcaaggcgg cacatttacg
     4681 atcaccaacg gcggcgtcta tggctcgttg atgtcgacgc cgatcctcaa tgcaccacag
     4741 tcggcgattc tcgggatgca caaaatcgag gaccggccgg tcgcgatcgg cggcaaggtc
     4801 gaaatccgcc cgatgatgta tctcgccttg acgtacgatc atcgcatcgt cgatggcaag
     4861 gaggcggtga ccttcctcgt ccgcgtcaaa gaggcgcttg aagacccggc gcggctgctg
     4921 ctcgatattt aatatttaaa agtgagcttc gagacgcgtt aatgcagtcg tgggttagga
     4981 ccagcgatcc atgtatgatc tcatcgtcat cggcaccggc cccggcggat atgtctgcgc
     5041 cattcgcgct gcgcaattgg gcctgaaggt cgccgtcgtc gaaaaaaaca agaccttcgg
     5101 cggcacttgt ctgaacatcg gctgcattcc ttcgaaggcg ctgcttgcag cttcggaaaa
     5161 attcgccgag gcggatcatg cgctcgcggg cttcggtgtc atcgtcgata agccgcggct
     5221 cgatctcaaa ggcatgatga agcacaagga cgataccgtc gcggccaacg tgaacggcgt
     5281 cgccttcctc ttcaagaaga acaagatcga cagctttatc ggggttggcg agatcaaaaa
     5341 ggccggcgtt gtcgaggtga cggcggacga cggtgcgaga cagagcctgg tgacgaaaaa
     5401 tatcgtcatt gccacgggct ccgatgtcgc gccgctgccc ggcctcgaga tcgacgagaa
     5461 gcaggtcgtc tcttcaactg gcgcactctc gctcggcaag gtgcccggca agatcatcgt
     5521 tgtcggcgcc ggtgtgatcg gcctcgagct tggttcggtc tggcggcggc ttggcgccga
     5581 agtgcttgtc gtcgaattcc tcgaccacat tctcggcccg atggatggcg aagtcggcaa
     5641 acaattccag cggatgctcg aaaagcaggg attcgaattc ctgctcggcc acaaggtcaa
     5701 caaagccgac aagacggggt caggcctcaa ggtcacgatc gctccggcaa acggcggcga
     5761 agagattatc cgcgatgcgg atatcatgct cgtcgcgatc ggccgccgtc cgtttatcgc
     5821 cgggctgggg ctggaagccg ccggcgtcgc gacggagcgc ggccgcgtcg tcatcgacga
     5881 acatttcgcc accaatgtgc cgggcatcta t
//
LOCUS       DASWOG010000523         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_233150,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000523 DASWOG010000000
VERSION     DASWOG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233150"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VGB93_10765"
     CDS             complement(<1..1432)
                     /locus_tag="VGB93_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY0146605.1"
                     /translation="MIVGFGILLILLATFGAILSVREFRIRDVAEARRELMALDLLFS
                     QETQRTMQSVDLVLSSLQDDLTLGGITKPQDIIRKRASVEMYLLLKSRIVGIPQIREA
                     SLIGPDGKLICTTKTYPAPNLDFSDRDYFLVLRDTPISGSYLSQPSYNASTKTWSIYL
                     ARRVDDASGHFIGVVAAAIDLTYLENLYRTLQIGDYGAVSLWRSDGTLLARYPASGSI
                     GQIYKIKSFEGILQPGTPVTFEATNTIDKPERIVATMAVDEFPLVVNISRLKSHVLAD
                     WRQARFLIAVGTLLFGIALCCVLWLLVRHFNTYDALTAADRERSKAIAERERAEAQLR
                     QAQKLESIGQLTGGIAHDFNNLLTAVLGNLEMLKRHTEKGDARLNRWANNAFDAAKRG
                     AVLTQRLLVFSRRQPLEPRGAKIESVLASMSDLLRRTLGENIEIVTTTEPGLWPAFAD
                     LNQLDNAILNIAINARDAMDGRGRLSI"
     gene            complement(1654..2637)
                     /locus_tag="VGB93_10770"
     CDS             complement(1654..2637)
                     /locus_tag="VGB93_10770"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0146606.1"
                     /translation="MTHTHILGTGSYAPSRILTNHDLEKIINTSDAWITERTGIKERR
                     IAADGEVTSDMAVAAARKALEMAETDPADLDMIIVGTISPDMPMPSCAVFVQAKLGAT
                     RAFAFDLSAACAGSLFGMAIADQFIRTGSARRVLVIGAELLSRLVDWEDRSSCVLFGD
                     AAGAMVLGPSPDPARGLISTHLHSDGTAAGMLSIPGGGSKYPQSEDVLAKKLNKISMN
                     GREVYKYARRALPEVILEALAANGLKPGAIDHVVTHQANIRIVESVVDRLGIPREKCW
                     LNIDRYGNTSSASLPISLDEANRAGRLRPGDLVAMMAIGAGMAWGSALVRW"
BASE COUNT          607 a          785 c          745 g          511 t
ORIGIN      
        1 tgatactcag gcggccgcgc ccgtccatcg cgtcacgtgc attgatcgcg atattgagaa
       61 tggcattgtc gagctggttg agatcggcga aagccggcca gagaccgggt tccgtcgtcg
      121 tgacaatctc aatgttttcc cccagcgtcc gtcgcagcaa gtcagacatc gaagcgagaa
      181 cgctctcgat cttcgcgccg cgcggctcaa gcggctgccg gcgcgaaaag acgagcaacc
      241 gctgtgtaag aacagcgcca cgcttggctg catcgaacgc attattcgcc caacgattga
      301 gccgtgcgtc gcccttctcc gtatggcgct tcagcatttc gaggttgccg agaaccgcag
      361 tcagcagatt attgaagtca tgcgcgatgc cgcccgttaa ctggccgatc gattcgagtt
      421 tctgtgcctg gcgcaattgc gcctcggccc gttcacgctc ggcaatcgcc ttgctgcgct
      481 cgcggtcagc cgccgtcaac gcatcgtaag tgttgaaatg gcgaacgagc agccagagaa
      541 cgcagcaaag ggcaatgccg aaaaggagag ttccgacagc gatgagaaac cgcgcctggc
      601 gccaatccgc cagaacatgg ctcttcagcc gcgaaatatt gacgaccagc ggaaattcgt
      661 cgaccgccat cgtcgcaacg atccgctccg gcttgtcgat cgtgtttgtc gcttcaaaag
      721 tgaccggcgt tcctggctgc aaaattcctt caaaggattt gatcttgtag atctgcccga
      781 tcgagcccga tgcagggtag cgcgccagca gcgtaccgtc gctgcgccat aggctgaccg
      841 caccataatc gccgatctgc agcgtcctat agagattttc gagataagtc agatcgatag
      901 ctgcggcgac gacaccgata aaatggcccg aggcatcatc gacacgtcgg gcaagataga
      961 tcgaccaggt ctttgtgctc gcgttatacg aaggctggct gagataagac ccgctgatcg
     1021 gtgtatcgcg cagaacaaga aaataatcgc gatccgagaa gtcgagattc ggcgccggat
     1081 aggttttggt cgtgcagatc agcttgccgt ccggacctat aagactggcc tcgcggattt
     1141 gcggaatacc gacgatccgc gatttcagca aaagatacat ttcgacgctg gcgcgcttgc
     1201 ggataatgtc ttgcggtttg gtaataccgc ccagcgtcag atcatcctga aggctcgaca
     1261 gaacgagatc gacgctctgc atcgtgcgtt gcgtctcttg cgagaacaaa agatcaagcg
     1321 ccatcaattc gcgccgcgcc tctgcaacgt cgcggatgcg aaattcgcgc acggacagga
     1381 tagcgccgaa cgtcgccagc aggatcagca gaatgccaaa cccgacgatc ataggttttg
     1441 gttttagcag cggagggcgc gaaccatccg cacgggcgcc atctaacggc aaggcatttc
     1501 ccgtcatgca ttgtgctcgg cctacggacc ccccgaggcc agcatcggca caatcgacag
     1561 atcaagttaa caggaccgga cgagaaagga acccatacca cgacggtaaa aaggtgatca
     1621 aaaatgacga tatttttcaa taagttatat ctactaccag cggacgagcg cactgcccca
     1681 ggccatcccg gcgccaatcg ccatcatcgc gacgagatcg cccgggcgca aacggccagc
     1741 gcgattcgcc tcatcaagcg aaatcggcaa cgacgcgctc gacgtattgc cgtagcgatc
     1801 gatgttcagc cagcattttt cacgcggaat gccgagccgg tcgacgaccg attcaacgat
     1861 gcggatgttc gcctggtgcg tgacgacatg atcgattgca ccaggcttga ggccattggc
     1921 ggcaagcgcc tcgagaatga cttcgggcaa cgcccgcctg gcgtatttgt agacttcgcg
     1981 accattcatc gaaattttgt tgagtttttt tgccagcacg tcctcagatt gcggatattt
     2041 actgccgccc cccggaatcg acagcatgcc agccgcggtg ccatcggaat ggagatgcgt
     2101 cgaaatcaga ccgcgcgccg ggtccggcga cgggccaagc accatcgcgc cagccgcgtc
     2161 accaaagaga acgcaactgc tccggtcctc ccaatcgacg agccggctca aaagctccgc
     2221 gccaatgacg agcacgcggc gcgcgctgcc ggtgcggatg aattgatcgg caatcgccat
     2281 gccaaacagt gacccggcac aagcggctga gagatcgaac gcaaaagccc gcgtcgcacc
     2341 aagtttcgcc tgaacgaaga cggcacaaga tggcatcggc atatcgggcg agatcgtccc
     2401 gacaatgatc atgtcgagat cggccggatc ggtttctgcc atctccaggg ctttacgcgc
     2461 tgcggcgacc gccatatccg acgtcacctc gccgtcggcc gcgatgcgac gctccttgat
     2521 ccccgtacgt tcggtgatcc acgcatccga agtattaatg atcttttcaa gatcgtgatt
     2581 ggtgaggatg cgcgacggtg cgtaagaccc cgtccccaaa atatgggtat gcgtcactgg
     2641 atatcttg
//
LOCUS       DASWOG010000524         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_12878,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000524 DASWOG010000000
VERSION     DASWOG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12878"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VGB93_10775"
     CDS             complement(<1..1466)
                     /locus_tag="VGB93_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146607.1"
                     /translation="MKDSSDGTRVSCHSRHVALAAAAAISAASFASPARADSANEKRL
                     EAKLDALMGEVSELKREMHVEKRHTQHEFEHVRMAVSAPGFAGPIVKGPGSYVPPQPW
                     DKRFHLFGITLTPGGFIEAGGVWRSRGDSTGIDTNWNAIPMGNSPLAHTNEFRFDARQ
                     SRFSLLAQGNINPALLVSGYSEFDFLAAAGTANQIDSNSYTPRVRVLYTTLDWTDTGW
                     SVLGGQSWTLATTNGKGIQPRTEIFAPFIDANFIPGSVWTRQPGLRITKNFGDFTAAL
                     SAEQPETTFAPACPGIGGYGAIGNGVSVYCQQAGSHFFNTATNYSLNRVPDAIAKVAW
                     DPTFGDRAMHFEAFGLYTDLYDRVEYPGGNFTSRDTTGWGAGGSVIVPILPKWLDFEA
                     NAMTGRGIGRYGVSTLPTATFNADGSLSPIPETIALAFLTLHATPAIDLWAAAGIEHE
                     YGDYFLGGTVNGPGSPLANNTGCNIETAAASTCNGITK"
     gene            complement(1897..2298)
                     /locus_tag="VGB93_10780"
     CDS             complement(1897..2298)
                     /locus_tag="VGB93_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0146608.1"
                     /translation="MAGLMRIFTWWQGATINTLFYTWRTGELVGEDEFGNRYYRTRGG
                     AIDPTLGFQRRWVIYKGYSEGSATPPGWYGWLHHTVDIAPPDDNYKPREWQLPHEPNM
                     TGTPLAWRPPGSTLASGVRPPATGDYEAWTP"
BASE COUNT          546 a          744 c          771 g          565 t
ORIGIN      
        1 tctttggtga tgccgttgca tgtgctggca gccgccgtct cgatattgca acccgtgttg
       61 ttggcgagcg gcgaacccgg cccgttcaca gtcccgccga ggaaatagtc gccgtattcg
      121 tgctcgatgc cggcggcggc ccagaggtcg atcgcgggcg tcgcatgcaa tgtcaagaaa
      181 gcgagcgcga tcgtttcggg tatcggcgac aatgaaccgt ctgcgttgaa ggttgcggtc
      241 ggcagcgtcg agacgccata gcggccgatg ccgcggcccg tcatcgcatt ggcttcaaag
      301 tcgagccact tcggcaaaat cggaacgatg acgctgccgc cggcgcccca acctgtcgta
      361 tcccggctcg tgaaattgcc gcccggatat tcgacgcgat cgtagagatc ggtgtagagg
      421 ccgaaggcct cgaaatgcat cgcgcgatcg ccgaatgttg gatcccaggc gacttttgcg
      481 atcgcatcgg gaacgcggtt gagcgagtag ttagtcgcag tgttgaagaa gtgcgaaccc
      541 gcctgctggc aataaacgct gacgccattg ccaattgctc catagccgcc aattcctgga
      601 caggccggcg cgaacgtcgt ctccggctgt tcagccgaga gcgccgccgt gaagtcaccg
      661 aagttcttag tgatgcgcag gcccggttga cgcgtccaca ccgagcccgg aatgaagttt
      721 gcgtcgataa acggcgcaaa gatttccgtg cgcggctgaa tacccttgcc gtttgtcgtc
      781 gccaacgtcc aggattggcc gcccagcacg ctccagccgg tgtccgtcca atccaaggtc
      841 gtgtaaagaa cgcgaacacg cggcgtgtag gaattgctgt cgatctgatt ggctgtaccg
      901 gcggcagcga gaaaatcgaa ttcgctgtag ccggagacca gcaacgccgg attgatgttg
      961 ccctgagcta acaacgagaa gcggctctga cgcgcatcga agcggaattc gttggtgtgc
     1021 gcgagcggcg aatttcccat ggggatggcg ttccaattgg tatcgatgcc ggtcgaatcg
     1081 ccgcgcgaac gccaaacacc acctgcttcg atgaagccgc cgggcgtcag ggtgatgcca
     1141 aagaggtgaa acctcttatc ccacggttgc ggtggaacgt atgaccccgg ccctttgacg
     1201 atgggaccag caaagcccgg cgcgctcact gccatgcgta cgtgttcaaa ttcgtgctgc
     1261 gtatggcgtt tctcaacgtg catttcgcgc ttcagctcac tgacctcgcc catcaaggcg
     1321 tcgagtttgg cttcaaggcg cttttcgttg gcggaatccg cccgcgccgg acttgcgaaa
     1381 cttgccgcgc tgatcgcggc ggcggcggcc agcgccacat gccgcgaatg gcaagacaca
     1441 cgtgtgccgt ctgatgaatc tttcatgcgt tccccctggt ccgtttcggc taacgccctg
     1501 cggtttttga agatttcttg tcggcgaatg ctcgccccaa aggattgccg ctccctggaa
     1561 ggagccgtcg tttatttgca atagggggag gctatgcgcg ccgggagcaa gtcgtaaaca
     1621 gactatatca atccaagcat aatatagtta taatattaat atcagtgttg catttatatc
     1681 ataagtaaat atagacctaa cgctcaactc tatttcaatc tttaggcgtt gcatattaaa
     1741 tatatattat taaatatatt tttatactta cggtcggcaa atgcttcgca aaaataaaaa
     1801 ggctgcgcaa agcgcagcct cgatcatcgg aaattaaact ctagaacggg gcctggtttt
     1861 gacacgcgtc gggaaaacag ggcccgtttg gcgcgcttag ggcgtccagg cttcgtaatc
     1921 gcccgttgct ggcggccgca cgccagaagc gagcgtcgag ccgggcggcc gccatgcgag
     1981 cggtgtgccg gtcatattcg gctcatgcgg caattgccat tcgcgcggct tataattgtc
     2041 gtcgggcggc gcgatgtcga cggtatgatg gagccagcca taccagccgg gcggcgtcgc
     2101 cgaaccttcg gaatagccct tataaatgac ccagcggcgc tggaacccca gcgttggatc
     2161 gatagcgcca ccgcgtgtgc gataatagcg attgccgaat tcgtcctccc cgaccagctc
     2221 gcctgtacgc caagtataga agagcgtgtt gatcgtcgcg ccctgccacc aggtgaaaat
     2281 acgcatgagg ccagccatga ggacggtttc cctgcttcga cgcggcgcaa tatgccagtg
     2341 acccagaaat tgtccacctt gcttgcgcaa gcctcagtca tgtttggcgc cggatcgttg
     2401 gccccagtcc cgacaatctg tggatgattc ccaggccgcc tgttgatgtt ggggatagcg
     2461 cggatggccg gcgcgaaagc cccgagagcc attgcggcgc acctccctcg gggtcaagat
     2521 tgcgttaaga gcgcggcctt gaattttaac tctttgcaca acattttgta tctaggcttc
     2581 gcgcgcctac tagagttagg cttattcaac agcgcgcgag tcggac
//
LOCUS       DASWOG010000525         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_177498,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000525 DASWOG010000000
VERSION     DASWOG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177498"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGB93_10785"
     CDS             complement(<1..261)
                     /locus_tag="VGB93_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146609.1"
                     /translation="MPKRLETLRRIASLQNDIRKTGEIKLATLQRQRFSAEATEARLV
                     SYLDENHIYTPAYMKTLADKLQAVGRAKRRLAQEIEDQAKVLL"
     gene            complement(263..1018)
                     /gene="fliR"
                     /locus_tag="VGB93_10790"
     CDS             complement(263..1018)
                     /gene="fliR"
                     /locus_tag="VGB93_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HEY0146610.1"
                     /translation="MIWTGSNAVLTAFLLFCRISGCLMLMPGFSSPRVPVTVRLFLCV
                     AISLALTPFLGETIGKIVDNASAFSLLKLIVSETLIGSLIGLMGRIFFAALETLGTSI
                     AMQIGLANALGVSVEDNESTPSIADFLIFLATTLLFVTDQHLEVIRGLVASYRTLPVA
                     SGFGSQFGLIQVADTLSKAFFLSLRVASPFILFGIVFNLAVGVANKLTPQIQVYFIAT
                     PFAVAGGLFLFYFTFKEVLGLFMAGFSNWLAGG"
     gene            complement(1015..>2876)
                     /gene="flhA"
                     /locus_tag="VGB93_10795"
     CDS             complement(1015..>2876)
                     /gene="flhA"
                     /locus_tag="VGB93_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY0146611.1"
                     /translation="VATLLRLSLNIATTRLILSNGPEGTSAAGYVIGGFAQLVMSGDF
                     VIGLIVFAILITVNFVVITKGATRIAEVGARFTLDAIPGKQMAIDADLSSGTIDEKEA
                     QRRRRELEEESSFFGSMDGASKFVRGDAIAGLIILAVNIFGGIIIGATRYDMPLSQAT
                     DVFTKLSVGDGLVTQIPALIISLAAGLLVSKGGTRGSAEQAVLGQLVKYPRALFVAGG
                     LMFVLGVIPGLPFVPFALLGSLMAFAGYTIPARHARQAAVDSASAAAAEQRALKEARE
                     SVKESLKSVEIELCLGKQLSSRMLAAQGELMHRVTKMRRKFAQQYGFVVPEIKVSDNL
                     MVPAKNYQIKIHGTVVASFTMMVGHLLVVVGDGRRPDLPGEEVREPAFGMKAMWVSEA
                     YAGELKRQGFESIDVMSVLLTHLSEVIRNNLSQLLSYKDMRALFDRLGPEYKRLLDDI
                     CPSQISYSGLQAVLKLLLAERVSIRNLQLILEAIAEIAPHVRRAEQVTEHVRMRIAQQ
                     ICGDLLDNGTLKVLRLGNRWDLAFHQSLKRDAKGDVIEFDLDPGMVEQFGNEASIAIR
                     DRMDKGHVFVLVTTPEARPYVRMVIERLFATLPVLSHLEIARGVEIKSLGTIS"
BASE COUNT          690 a          841 c          807 g          538 t
ORIGIN      
        1 gagcagaact ttcgcctgat cttcgatctc ttgtgccagc cggcgcttgg cgcgtcccac
       61 tgcctgcagt ttatcggcaa gagttttcat gtaggcgggc gtgtaaatat gattctcgtc
      121 gagatatgag acaagccgcg cctcggtcgc ttcggcgctg aaccgctgac gctgaagcgt
      181 cgcgagctta atttcgccgg tcttgcggat gtcattttgc aggctcgcga tccgacgcag
      241 cgtctcaagt ctcttcggca tctcaaccgc ccgccagcca attcgagaac cccgccatga
      301 aaagcccaag cacctctttg aaggtgaagt aaaagaggaa caggccgccg gccaccgcaa
      361 atggcgtggc aatgaaatat acctggatct gcggcgtgag cttattggcg acaccgacag
      421 cgagattgaa gacgatgccg aacaaaatga atggactcgc gactctcaga ctgagaaaga
      481 aggccttcga cagcgtgtcg gcaacctgga taagtccgaa ttgggagcca aatccggagg
      541 cgaccgggag cgtgcggtaa gacgcgacaa ggccgcgtat gacttccagg tgctgatcgg
      601 tgacgaagag caaggtcgtc gcaaggaaga ttaggaaatc cgcgatcgac ggtgtggact
      661 cgttgtcctc gaccgacacg cccagtgcgt tggcgagacc gatctgcatc gcgatcgacg
      721 tgccgagcgt ttcgagcgcc gcgaaaaaaa tacgtcccat aaggccgatc aagctgccga
      781 tgagcgtttc cgaaacgatg agcttcaaga gcgaaaatgc ggaggcgtta tcgacgatct
      841 tcccgatcgt ttcaccaagg aacggcgtca aagccagact gatcgcaacg cacagaaaga
      901 gccgcacggt gaccgggaca cgcggacttg aaaatcccgg catcagcatg aggcacccgg
      961 agatgcggca gaaaagaagg aacgccgtca ggaccgcgtt cgatccagtc cagatcacga
     1021 gattgttcca agcgacttga tctcgacgcc gcgggcgatc tccagatgcg agagaaccgg
     1081 caatgttgca aacaaccgct caatcaccat gcgcacgtaa ggccgtgctt ccggcgttgt
     1141 aacgaggaca aagacatgcc ccttgtccat ccggtcgcgg atcgcgatcg acgcctcgtt
     1201 gccgaattgt tcgaccatac cggggtctag gtcgaactcg atcacatcgc ctttcgcatc
     1261 acgtttcaag ctttggtgga acgccagatc ccagcgattg ccaagccgca gcactttcaa
     1321 cgtgccgttg tcgaggaggt cgccgcaaat ctgttgcgct atgcgcatgc gcacatgctc
     1381 tgtcacctgc tcggcgcgac gcacatgcgg cgcaatctca gcgatggctt caaggatcaa
     1441 ttgcaaattg cggatcgaga ctcgctccgc gagcaaaagc ttgaggactg cctgcaggcc
     1501 ggaataggaa atttgcgaag gacaaatatc gtcaagcaac cgcttgtatt cgggtccaag
     1561 ccgatcgaac agcgcacgca tgtccttgta agagagcaat tgcgagagat tattgcggat
     1621 gacctcgctc aaatgcgtga gcagaaccga catcacgtca atcgattcga agccttggcg
     1681 tttgagttcg cccgcatagg cttcagaaac ccacatcgcc ttcatcccaa aggcaggctc
     1741 acgcacctct tcgcccggca gatccggcct gcggccgtca ccgacaacga caagcaaatg
     1801 gccgaccatc atagtgaacg acgcgaccac cgttccgtga attttgatct gataattctt
     1861 cgccggcacc atcaggttat ctgagacctt gatctccggg acaacgaagc cgtattgctg
     1921 ggcgaacttt cgccgcatct tggtgacgcg atgcatgagt tcgccttgcg ccgcgagcat
     1981 acgcgaggaa agctgcttgc cgaggcaaag ctcgatctca accgacttca gcgattcctt
     2041 gacggattca cgcgcctcct tcagcgccct ttgttcggcc gccgcagccg acgcgctgtc
     2101 caccgccgcc tgccgtgcgt ggcgcgccgg gattgtatag ccggcgaacg ccatgagact
     2161 acccagtaag gcaaatggga cgaacggcag ccccggaata acaccaagta caaacatcaa
     2221 cccgcccgca acgaagagcg cacgcgggta ttttaccaac tggccgagca ccgcctgctc
     2281 ggccgagcca cgcgtcccgc cttttgagac gagaagaccg gccgccagcg agatgatcag
     2341 ggccggaatt tgcgtgacga gaccgtcgcc gaccgagagc tttgtgaaaa catctgtggc
     2401 ctgcgacaaa ggcatatcat agcgggtcgc cccgatgatg atgccgccga aaatattgac
     2461 cgcaagaata atgagaccgg cgattgcgtc accacgcacg aacttggacg caccgtccat
     2521 cgaaccgaaa aaggagcttt cctcttcaag ttcgcgacga cgacgctgcg cctccttttc
     2581 atcgatcgtt cccgatgaga gatcggcgtc gatcgccatt tgtttgccag ggatggcgtc
     2641 aagtgtaaaa cgcgcgccga cttccgcgat acgcgtcgcg cctttggtta tgacgacaaa
     2701 attgaccgta atgaggattg caaaaacaat aagcccgatg acgaaatcgc cgctcatgac
     2761 gagctgggca aagccgccga tgacatagcc cgcggcggag gtgccctcag gcccatttga
     2821 aagaatgagt cgtgtcgtgg cgatattcag cgacagccgc aacagcgtcg cgacga
//
LOCUS       DASWOG010000526         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_31276,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000526 DASWOG010000000
VERSION     DASWOG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31276"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGB93_10800"
     CDS             <1..327
                     /locus_tag="VGB93_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEY0146612.1"
                     /translation="NYGLSRWTAFTHFLEDGRVCLSNNAAERAVRGIAVGRRNWTFAG
                     SDEGGRRAASVYTLIETCKLNDVDPQAWLTDVLARLPDHPAKEISQLLPWAWKAARQS
                     AQAVAA"
     gene            complement(271..849)
                     /locus_tag="VGB93_10805"
     CDS             complement(271..849)
                     /locus_tag="VGB93_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146613.1"
                     /translation="MMSAIVRRRFIIFAVFPLLLCGLAYVVSLTHSKYSQAAYEFLAF
                     LLIVDLAIGLHGGLRNTATAIAATVLGLSLIEVAATAMQPDQAINTRGFSVSRPILGW
                     GPSAPGTYHAARTGVDGRLIYNVDYTIDNQLLRRTQSGPGDPTVAFFGDSFTFGVRIR
                     FAPQVDLGSTKGSCLGGHSLRGLARSFPCPWQ"
     gene            1067..1759
                     /locus_tag="VGB93_10810"
     CDS             1067..1759
                     /locus_tag="VGB93_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0146614.1"
                     /translation="MNIEGSVALVTGANRGLGKVLVEQLRACGAKKIYAGARDPSKVA
                     HSGVEPVKLDITSAADVEAAAKALSDVTLVINNAGVASFSSLADDGSLEIARKAMETN
                     YFGTLRVANAFAPILAKNGGGALVNMLSVLTWITYPSLAAYCASKSAAWALTNALRQE
                     LKAQGTLVVGVHAGLIDTDMAAGVEAPKVTPEQVAKAILDGVAAGHEEVLSDDPSRHI
                     KAALSNQPGPYL"
BASE COUNT          380 a          531 c          519 g          340 t
ORIGIN      
        1 aattatggcc tcagccgctg gactgccttc acacacttcc tggaggatgg ccgcgtttgc
       61 ctcagcaata atgccgccga acgcgcggtg cgtggaatcg ccgtcggccg ccgcaactgg
      121 acattcgcgg gctccgacga aggcggccgc cgtgccgcat cggtttatac attgatagaa
      181 acctgtaagc tgaacgacgt cgatccgcag gcttggctca ccgacgtcct ggcgcgcctc
      241 cccgaccatc cggcaaagga gatcagccaa ctattgccat gggcatggaa agctgcgcgc
      301 caatccgcgc aggctgtggc cgcctaaaca actacccttc gtcgaaccca gatccacctg
      361 cggtgcaaac cggatgcgta cgccgaatgt gaacgagtcg ccgaagaagg cgacagtggg
      421 atcgcccgga cctgattgcg ttcgacgcaa cagctgatta tcgatcgtgt aatcgacgtt
      481 gtagatcaga cgtccgtcga cgcccgttct ggcggcgtga taggtgccgg gagcagaagg
      541 cccccagccc aaaattggcc gggaaacgga gaaaccccgc gtattgatgg cctggtcggg
      601 ttgcatggcg gttgccgcga cctcgatcaa ggacaatccc aagactgtcg cggctatcgc
      661 agtcgctgtg ttccgcaacc cgccatgaag tccgatcgct aaatcgacaa tcagcaggaa
      721 ggccagaaat tcgtatgctg cctgagaata cttggagtgg gtcagcgata cgacataggc
      781 cagtccgcaa agtaacagcg gaaatacggc aaagataata aaccggcgtc gaacaattgc
      841 tgacatcatc accccgatat attttccctc gcgtctgtgc accttcttaa ctgctctgtg
      901 gtgcgcacgc gagaccggtg agaccgcaaa aatttcgaag ggagttgcgg tccgcgacct
      961 tcaggatatt ccagagttct ttcagcctct ggcttgaccc gcgagaaatc gggtgcatat
     1021 acatccatac aagcgcatat gcgcctattg gctcatggag agacggatga atatcgaagg
     1081 atcggtcgcg ttggtcacgg gcgccaaccg cggccttggc aaagtcttgg ttgagcaatt
     1141 gcgcgcgtgc ggcgccaaga aaatctacgc tggagcgcgc gacccgtcga aggtcgctca
     1201 ttctggcgtt gagccggtga aactcgatat tacgagtgcc gccgacgtcg aggctgcggc
     1261 caaggccctg agcgacgtca ccctggtcat caacaatgcc ggcgttgcgt ctttttcctc
     1321 gcttgccgac gacggctcgc tggagatcgc tcgaaaggcg atggaaacca attatttcgg
     1381 cactttacgc gtcgccaatg ctttcgcgcc gatcctcgcc aagaatggcg gcggcgccct
     1441 cgtcaacatg ctctccgtct tgacctggat cacctatcca tcgcttgccg cctattgcgc
     1501 gtcgaaatcg gctgcctggg cgctcaccaa tgccctgcgg caggaattga aagcgcaagg
     1561 cacgctggtt gtcggggttc acgccggatt gatcgacacc gatatggccg ccggggtcga
     1621 agcgccgaag gtcacgccgg aacaggtcgc gaaggccatc ctcgatgggg tcgcggcggg
     1681 gcacgaggag gtactttccg acgatccctc gcgccatatc aaggcagcgc tttcgaacca
     1741 gccggggcca tacctctaag ccggcgtcac
//
LOCUS       DASWOG010000527         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_48905,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000527 DASWOG010000000
VERSION     DASWOG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48905"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VGB93_10815"
     CDS             complement(<1..113)
                     /locus_tag="VGB93_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HEY0146615.1"
                     /translation="MSPIISVSHLSKTYSGGRAALKDVSLEIGKGEIFALLG"
     gene            complement(197..1492)
                     /locus_tag="VGB93_10820"
     CDS             complement(197..1492)
                     /locus_tag="VGB93_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146616.1"
                     /translation="MNMRAKTSYPFGQKYVFVVVAAIFLALLAAAGLRATPGVLLLPW
                     QKAFGWSAGTVSAAAALGIFLYGLTGPFAAAAMQQFGLRRTVVAAIVLMALAAGISVF
                     MTAPWQLFLTWGVLSGLGSGVVANVLAATIVNRWFKTHRGLIMGLLTASMSTGTLIFL
                     PGLAGLAAWGGWKPVVLTIAGCALALIPLIAFLVPESPAAIGLRRYGATEDEPAAPAR
                     AHNPFTVALGDLAMAARTRTFWFLFATFFICGFTTNGLVGTHLIAFCGDNGIAEVHAA
                     GLLAMMGIFDIFGTTFSGWLTDRYDPRKLLFLYYGLRGLSLIYLPYSNFSFVSLSLFA
                     AFYGLDWIATVPPTVRIANDKFGDKNALIIFGWIVAGHQLCAASAALLAGLIRTAQGS
                     YLEAFVIAGATGLAAAFLSLMIEKQKVAPQFAGALEAGE"
     gene            1563..2162
                     /locus_tag="VGB93_10825"
     CDS             1563..2162
                     /locus_tag="VGB93_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0146617.1"
                     /translation="MSARTDKSRRKAGGDTPRAADRIRASARELFYREGIRAVGVDEI
                     VHRAGVTKPSLYRSFASKDELAADYLRDYDRDFWERFERPHGKAYADPREQVLAYIAE
                     LSGRAVSRGYRGCGLSNAAIEYPTPDHPARKVAEAHKKAVRQRLRDLAAAMDAREPDV
                     LGDSLLLLIEGIFGSGQQSEDGPAQSAVAAAERLIDSFT"
     gene            2238..2672
                     /locus_tag="VGB93_10830"
     CDS             2238..2672
                     /locus_tag="VGB93_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0146618.1"
                     /translation="MDEKAYNFFDLAEIKARFPDRAESMIVDAYLSDHESASSRLFRL
                     YHPIPRHLHETCDEHLVLLDGEVDFTIADEPPRWLRAGQMVTFLRNIVHGIQPVEAAA
                     PAVFLTVDTPRRAPGDVHFVDPTEAKGRRFVIHLASYEARSA"
     gene            2817..>3294
                     /locus_tag="VGB93_10835"
     CDS             2817..>3294
                     /locus_tag="VGB93_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0146619.1"
                     /translation="MSQANLRLVEGTSVDKTKALDAAVSQIERAFGKGSIMRLGKSPV
                     VAIETISTGSLGLDIALGVGGLPRGRVVEIYGPASSGKTTLTLHVIAEAQKAGGVCAF
                     IDAEHALDPIYARKLGGNLDDLLISQPDTGEQALEITDTLVRSGSVDVPVIDSVAAL"
BASE COUNT          689 a          976 c         1042 g          587 t
ORIGIN      
        1 ccgagcaggg cgaaaatttc gcctttaccg atctcaaggc tcacgtcctt cagcgccgcc
       61 cggccgccgg aataggtttt cgaaagatgg ctgaccgaga tgatgggcga catgaccgac
      121 tggatggggg gtgggcgact cgggggccgc ttttagagca tcgctccccg aagtggaaat
      181 ccgatttgcg tcccacctat tcgccggcct ccaaagcgcc ggcaaactgc ggcgcaacct
      241 tttgcttttc aatcatgagt gacagaaatg cggcggcgag gccggtggca ccggcgatga
      301 cgaacgcctc aagatagctg ccttgcgcgg tacggataag gccggctagg agagccgcac
      361 tcgcagcgca aagctggtgg ccggcgacga tccagccgaa gatgatcagc gcgttcttgt
      421 cgccgaactt gtcattggcg atgcgcactg tcgggggcac cgtcgcgatc cagtcgaggc
      481 catagaaagc ggcgaacagc gaaaggctta cgaacgaaaa gttcgaatac ggcagataga
      541 tcagcgagag gccgcgcagc ccgtaataaa ggaacagcag tttgcgcggg tcatagcgat
      601 cggtcagcca acctgaaaac gtcgtgccga aaatatcgaa aatgcccatc atcgccagca
      661 ggcctgcggc gtggacctca gcgatgccgt tgtcgccgca aaaggcaatg aggtgcgtgc
      721 cgacgagtcc gttggtcgtg aagccgcaga tgaagaatgt cgcgaataga aaccagaagg
      781 ttcgcgtccg tgccgccatt gccaaatcgc cgagcgcgac agtgaaggga ttatgcgcac
      841 gggccggggc ggctggctcg tcctcggtcg cgccgtagcg ccgcaagccg atcgccgccg
      901 gactttccgg aacgaggaac gcgatcaatg gaatgagcgc gagggcgcag ccggcgatcg
      961 tcaggaccac cggcttccag ccgccccagg cggcgaggcc cgcgaggcca ggcagaaaga
     1021 taagcgttcc cgtcgacatg ctcgctgtca gcaggcccat gatcaggccg cgatgcgttt
     1081 tgaaccaacg gttgacgatt gtggcggcca gcacgttggc aacgacgccc gaaccgaggc
     1141 cggagagcac gccccatgtc agaaagagct gccatggcgc ggtcatgaag acgctgatgc
     1201 ctgcggccaa agccatcagg acaatggcgg cgacgacggt tcggcgcagg ccgaattgct
     1261 gcatggctgc cgcagcaaag ggacctgtga ggccataaag gaaaatgccg agggcggcgg
     1321 cggcggagac cgtcccggcg ctccagccga acgctttttg ccagggtagc agcagcacgc
     1381 cgggcgtcgc gcgtaggccc gctgcagcga gcagggcgag aaaaatcgcg gcgacgacga
     1441 cgaacacata cttctggccg aagggataac ttgttttggc gcgcatgttc atgttactga
     1501 ccggtacgtg tcgggcttaa tacgtaccga gcggtaacat tgtcaagacg cattggatgc
     1561 ggatgagcgc gcggacggac aaatcgagac gtaaagccgg aggcgatacg ccacgcgcgg
     1621 ccgaccgtat ccgcgcctcg gcgcgcgaat tattttatcg cgagggtatt cgtgcggtcg
     1681 gcgtggacga gattgttcac cgcgctggcg tcaccaagcc gagcctctat cgcagctttg
     1741 cttccaagga cgaactcgcg gccgactatc tgcgcgatta cgatcgcgat ttctgggaaa
     1801 ggttcgagcg cccgcacggg aaggcttatg ccgacccgcg cgagcaggtt ctcgcttata
     1861 ttgccgaatt gtcggggcgg gccgtcagtc gcggctatcg cggctgcggg ctcagcaatg
     1921 cggcgatcga atatccgacg cccgaccatc ccgcccgcaa ggtcgccgaa gcgcataaga
     1981 aggcagtccg ccagcgtctg cgcgacctcg ccgcggcgat ggacgcccgc gagccagatg
     2041 tcctcggcga cagcctgctt ctgctgatcg agggcatctt tggctccggt cagcagtcag
     2101 aggatggccc ggcgcaatcc gccgtcgcgg cggcagaacg tctcatcgac agtttcacat
     2161 agggccgtga acagtcgggc gcggatacat agatatgagg cgttccgagc ccgcccatca
     2221 ccggagtcca aagcgcgatg gatgaaaaag cttataattt cttcgatctg gcagaaatca
     2281 aagctcgctt tcctgatcgt gccgagtcga tgattgtcga tgcctacctg tcggaccacg
     2341 agtctgcgag ttcgcggttg ttccgcctct atcatccgat cccgcgtcac cttcacgaga
     2401 cctgcgacga gcatctcgtt ctgctcgacg gcgaagttga tttcaccatt gccgacgagc
     2461 cgccgcgctg gcttcgcgcg gggcagatgg tgacctttct gcgaaacatt gtgcatggga
     2521 ttcagccggt cgaagctgcg gcacccgccg ttttcttaac tgttgatacg ccgcgccggg
     2581 cacccggcga cgtgcatttc gttgatccca ccgaggccaa gggacggcgt ttcgtgatcc
     2641 atcttgccag ctatgaagcg cgatcggcat aaagcctcga taatggcagg aaagaacaaa
     2701 aaaagaactt ggtcgacaag aacaaatagc gtacatttct cggcacaggc ggaagcgtcg
     2761 ccgccgcgca aacacattgc acttgtccgc acgcccgtgt cagaaggaat cattcgatga
     2821 gccaagcgaa tcttcgcctt gtggaaggaa catccgtgga caagaccaag gcgctcgacg
     2881 ccgccgtctc tcaaatcgag cgcgcgtttg gcaaaggctc gatcatgcgc ctcggcaaga
     2941 gcccggtggt cgcgatcgag acgatttcga cggggtcgct cgggcttgat atcgcgcttg
     3001 gtgtcggcgg tctgccgcgc ggccgggtcg tcgagattta tggcccggca tcttccggca
     3061 agacgacgct gacgctgcat gtcatcgccg aggcccagaa ggccggcggc gtctgcgcct
     3121 tcatcgatgc cgaacatgcg cttgatccga tctatgcccg caaacttggc ggcaatctcg
     3181 acgatctgtt gatctcgcag cccgatacgg gcgaacaggc gcttgaaatt accgatactc
     3241 tggttcgctc cggctcggtt gacgtgccgg tgatcgactc ggttgccgcc ctca
//
LOCUS       DASWOG010000528         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_287448,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000528 DASWOG010000000
VERSION     DASWOG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287448"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGB93_10840"
     CDS             <1..334
                     /locus_tag="VGB93_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY0146620.1"
                     /translation="ASWIIWGPPPAFAYALVAAVSVVIIACPCALGLATPMSIMVGVG
                     KGATAGVLIKNAEALERFEKIDTLVVDKTGTLTEGNPRVLSVKPSEGFDETALLALAA
                     SLERARAW"
     gene            420..1363
                     /locus_tag="VGB93_10845"
     CDS             join(420..755,758..1363)
                     /locus_tag="VGB93_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611189.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0146621.1"
                     /translation="MGRPYSMDLRERVVAAIVGGMSCRQAAARFGVSYSVAIGWMKRL
                     RETGSVAPGQMGGHKPRKISGEYRTWLVERCRERAFTLHGLVVELGARGLVVDYRSVW
                     DFVHGEGLTYKKTMVATERERPDVARRRAQWLKHRIGIDPARLVFIDETWTKTNMAPL
                     RGWAPRGERLIGHAPFGHWNTMTFLAALRHDRIDAPWLIDGPINGERFLVYVEKVLVP
                     TLSPGDIVIMDNLGSHKAKAVRLAIRQAGARLFFLPKYSPDLNPIEKFFAKLKHLLRK
                     AAARTIDAVCQAIAEILDTASPQECRNYFIEAGYDQT"
     gene            1381..>1827
                     /locus_tag="VGB93_10850"
     CDS             1381..>1827
                     /locus_tag="VGB93_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0146622.1"
                     /translation="MHSLAAAIVEAARERGLGLHEVTDFSSDTGKGVKGAVAGKLVAI
                     GNAKLLAEAGTAVPASLEEQADLLRREGATVMYVTVDGKSAGIVAVADPVKASTPAAL
                     ESLRAAGSRIVMLTGDNRKTAVAVANKLGITEIEAEVLPEQKNQIVR"
BASE COUNT          345 a          550 c          592 g          340 t
ORIGIN      
        1 cgcgagctgg ataatctggg gcccgccgcc agcctttgcc tatgcactgg tcgccgctgt
       61 atcggtcgtc atcatcgcct gcccctgcgc gctcggcctg gcgacaccga tgtccatcat
      121 ggtcggcgtc gggaagggcg cgacagccgg cgttctcatc aagaatgcag aggcgctcga
      181 gcgcttcgaa aagatcgaca cgctggtcgt ggataagacc ggcaccctga cggaggggaa
      241 tccacgcgtg ctttctgtca aacccagcga aggcttcgat gagacggcgc tcctcgcact
      301 tgcggccagc ctcgaacggg ctagagcgtg gtgaaatttg gttgagtcgg tggatttagg
      361 cttttcaaga tgttgtattt ttgattcaag aatcgggctg gatatggggg ccagcccgga
      421 tgggacgtcc ttattcgatg gatcttcgag agcgcgtcgt tgcagcgatt gtgggcggga
      481 tgtcgtgtcg gcaggcggcc gcccgcttcg gtgtcagcta cagcgtcgcg attggctgga
      541 tgaagcgtct tcgcgagacg gggagtgtgg cgcccggcca gatgggcggt cacaagccga
      601 ggaagatttc gggcgagtac cgcacctggc ttgtggagcg ctgccgcgag cgtgccttca
      661 ccttgcatgg cctcgtcgtc gagctgggcg cccgcggtct tgtggtcgat taccgctcgg
      721 tctgggactt cgtccatggc gaggggctca catataaaaa aagacgatgg tcgcgaccga
      781 acgggagcgg ccggacgtcg cccgtcggcg agcgcagtgg ctgaaacatc gcatcggcat
      841 cgatccggcc cggctggttt tcatcgacga gacttggacc aagaccaaca tggcgccgct
      901 gcggggctgg gcgccgcgcg gtgagcgcct gatcggtcat gctcctttcg gccattggaa
      961 tacgatgact ttcctggcgg cgttgcgcca tgaccggatc gacgcgccct ggctcatcga
     1021 cggaccgatc aacggcgagc gcttcctcgt ctatgtcgaa aaggtgctcg tcccgacctt
     1081 gagcccaggc gatatcgtga tcatggacaa tctcggttcc cacaaggcca aggccgttcg
     1141 tcttgcaatc cgacaggccg gcgccaggct gttcttcctg ccgaaatact cccccgatct
     1201 gaacccgatc gagaagttct tcgctaagct caagcatctc ttgcgaaagg ccgccgcgag
     1261 aaccatcgat gccgtctgcc aggccatcgc cgaaatcctc gatacagcct ccccgcagga
     1321 atgccgcaac tacttcatcg aagcaggata tgaccaaacc taatttcata ccgctctagc
     1381 gtgcattcgc tcgcagcggc gatcgtcgag gcggcacgcg agcgcggcct cggcttgcac
     1441 gaggtcaccg atttcagctc ggatacgggc aagggcgtca agggcgcagt tgcagggaag
     1501 ctggtggcga tcggcaatgc caagctcctg gcagaggccg gcacagccgt gccggccagc
     1561 ttagaagagc aggcggatct cctccgcaga gaaggcgcca ccgtcatgta cgttacagtc
     1621 gacggcaagt cggccggcat cgtagcggtc gccgatccgg tcaaagcctc gaccccggca
     1681 gccttggaaa gcttgcgcgc cgccggcagc cgcatcgtca tgctgaccgg cgacaaccgc
     1741 aagacggcag tggcggtggc caacaagctc gggatcactg aaatcgaggc cgaggtgctg
     1801 ccggagcaga agaaccagat cgtgcgg
//
LOCUS       DASWOG010000529        10984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_177675,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000529 DASWOG010000000
VERSION     DASWOG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177675"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VGB93_10855"
     CDS             complement(<1..1020)
                     /locus_tag="VGB93_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0146623.1"
                     /translation="MGGRAFIAVPIFIGALIGSTFEFSAVNAQQASAGGTEALPHIDV
                     TAPPGGDNIQSTPPLQQVPQVGLTGTKVSDLPLSVKVVPSQLVTQQGGTDVKDALRDV
                     SGTSTGGPDSIGGFDRFLIRGLDARIYEDGFSDGEQVNGLPHSLNGVQSIEALKGPGS
                     ALLGSGPPGGSINITHYLPSSTFSGGVGAQYGSFNTINSNYWLTGPTLVNGLSYRVDA
                     TVAHSDGFRGLKSDDYEIRPELTWNLGDHFITFSVDARHIIGTPDSYGLIYFHGSPID
                     VSRDAKYSTPFGYVDQDYLRTDLSDAWTINKFLTLNNRFSYLRRDASFLRNNDSGTVV
                     GDTFTG"
     gene            complement(1429..2553)
                     /locus_tag="VGB93_10860"
     CDS             complement(1429..2553)
                     /locus_tag="VGB93_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0146624.1"
                     /translation="MSTPALVIGGGLAGAGLAVSLANAGRAVTLVERETRGQHKVCGE
                     FVSAEAMLYLRDLGIDLDGLGAVAIERVGMFSGERQATAYLPFSARSLSRYALDEALL
                     NRAARCGARVLRGHKAIGVERDGHRWRALLDDGTSVSASEIFLATGKHDLRGWKRPPG
                     SQNDLIGFKLHWRLSASQTQALRGAVELFLFPGGYAGLEAVENDVANLCLVVRRRTFA
                     CGQSWPTLFASLLKNCPHLRRRLDGAQPCWPRPLAISAIPYGFVQRRADGLWRLGDQA
                     ACTPSFSGDGMSIALHSARLAAQYYAQGHDARTFQARLAQDVAPQVQRATLLSQMLLQ
                     PLGQKAGMATLERLPSLLRVVARATRLPQNRIDAARAGSV"
     gene            complement(2550..3272)
                     /locus_tag="VGB93_10865"
     CDS             complement(2550..3272)
                     /locus_tag="VGB93_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0146625.1"
                     /translation="MIALDFSRRNDLSELMDAEDTDFATFRECLVDLTKVNRLTLAYR
                     PTLSFLKRLAVSGALPRNRAVSVVDVGSGSGDMLRRIDLWATAEGYKFDLVGVDLNPW
                     SAKTAAQLTQENPRLRFVTANIFDYRPAAPIDIVISSLFTHHLDNEAIVKFLCWMEAN
                     TRIGWFVNDLQRHPLAYHAFKHASHALRFHHFVQHDGPVSIARAFSADDWQQLLGAAD
                     ISGARVRGHVPFRLCVSQIKPK"
     gene            complement(3272..4345)
                     /locus_tag="VGB93_10870"
     CDS             complement(3272..4345)
                     /locus_tag="VGB93_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY0146626.1"
                     /translation="MTIAYINRVATAVPAHEVHADFVRFAGQTLQNRTSRALFERMAN
                     RAQIERRWSCLAPPNGGVEVLDGEGFYKPGHFPSTADRMARYAFAAPALGATAVERLG
                     LDDSERKGITHLIVASCTGFSAPGVDLELLERCGLSSSVERTVIGFMGCHAAMNALKI
                     AHHIVRSTPTANVLVVCLELCTLHFQETADLDRLLTFLLFGDGCAAALISAAPDGIAL
                     DSFHAELVQKAADQITWNIRDAGFDMVLSGRVPSTITGALRESSARVLGGKPMEAIDI
                     WAVHPGGRSILDAVEGAFELEDSALAASRAVLRDYGNMSSATILFVLERLMRDKTKRG
                     ASGCALAFGPGLTAETMLFRMAA"
     gene            complement(4375..4803)
                     /locus_tag="VGB93_10875"
     CDS             complement(4375..4803)
                     /locus_tag="VGB93_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146627.1"
                     /translation="MLRKSALALIAAAALSVSASGAWAHGGGGGGGGHGGGFGGGGFG
                     GGGFHGGGGFGGSSFGHAGFAGGGFGHAGGGHFAAIGRPGFHDGHSFHGHFRGRGFAR
                     NGFFGDPFWYDGDYDDSSYDSACYREVWTRWGWTPAWVCN"
     gene            5004..5696
                     /locus_tag="VGB93_10880"
     CDS             5004..5696
                     /locus_tag="VGB93_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146628.1"
                     /translation="MAENRRRILIIEDDRETAEQLVEFVETSGYEVDLALNGEEGFER
                     GRSPDYAVMVIDRMLPSIDGIAIIRRLREIGIVTPTLIISALGEVDDKVRGLRAGGDD
                     YLVKPFAFTELLARVDALARRSTAAAKDLVLRVGDLTLDLVSRSVTRAGREIELLPRE
                     FQVLEYLVRNEGHVVPRAMLLQHVWDLHFDPTTNIIDVYVGRVRRKVDSLRDYPLIHT
                     VRGVGFCVRAPS"
     gene            5683..7089
                     /locus_tag="VGB93_10885"
     CDS             5683..7089
                     /locus_tag="VGB93_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0146629.1"
                     /translation="MLLAKTFRSRTFRIALTAIAGFGTIVLSLLGYIYWSTTSYVASR
                     SDRAIAVEQSLLQAAYDQGGRGALEAAIKTRIAEKAFNGGLYLLADQNFKPVAGDLDS
                     WPHELSGASGFGNFGAPDTGGRSSYRALIDTLPDNSHLLVGRNAEDLESFKRTIDTAL
                     AFTLLLIAVLAGVVSILVTRRTVGRIESINATSRAIMERGLAARIGLRGVNDEWDELA
                     RNLNSMLDRIEALMREVREVTDNVAHDLRTPLARIRGRLEKASGSPRSAESDQKLIDD
                     MIGDLDDVLRIFSSLMRISQIESAARTEGFAVVDLAAVARDVVELFDAAAEEKPIELS
                     VAGDPCVVVTGDRDLLFDAVANLVDNAIKYGKKNGLVTVTVRALEHDALIAVADDGPG
                     IPSDEFAHIFKRFYRLERSRCTAGNGLGLSLVAAVAHLHRARIDLKDNQPGLAFRLII
                     PSGDAAFGEKVSEGNAQG"
     gene            complement(7101..7937)
                     /locus_tag="VGB93_10890"
     CDS             complement(7101..7937)
                     /locus_tag="VGB93_10890"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0146630.1"
                     /translation="MGNLDGLRILIPESRQLNLFVNMIEEQGGTAIRCPLVQILDLED
                     SREAEAWIDKLIAGGFEDVIWLTGEGLRRLLAIAQRTERETAFIAALGNVRSITRGPK
                     PARALREIGLAPALAAATPTSEGVAEALAPEDIAGRPIGVQLYPGDGPNALLATLQTR
                     GAVLTPVTPYRYASQAETAQVVGAIEEMAAGHIDVIAFTSTPQVERLFDVAEKSGLGP
                     KLKEAFSHVSVASIGPIVEEALHRHGILQMVQPEAAFHMKPLLRTIAAWNADRSISQI
                     ET"
     gene            8054..9496
                     /locus_tag="VGB93_10895"
     CDS             8054..9496
                     /locus_tag="VGB93_10895"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0146631.1"
                     /translation="MTETYPDVQLLIDGKWRPGASGQTIPVINPATEEVIGQVAHADI
                     ADLDLALAAAKRGFAVWRNVSAFERGKILRRAGELLRERAEHVALLMTREQGKPLTEA
                     RVEVMAAADTLDWFGEEARRAYGRIVPARAPNVTNLVMKEPVGPVAAFAPWNFPLNQT
                     VRKLGAALAAGCSIIVKAPEETPASPAELIRAVIDAGVPDGVVNLVYGVPAEISEHLI
                     PHEAIRKISFTGSTAVGKQLAALAGKHMKRITMELGGHAPAIVFDDADVDAAAKLLVA
                     AKYRNGGQVCTAPTRFLVQRKVYEPFLDRFVAAAQSLKVGNGTAPDTQLGPLANPRRI
                     DAMEALIADALGKGAELRAGGARLGNKGYFFAATVLVNVPLTARAMNDEPFGPLALVN
                     AFDDLDEALAEGNRLPYALASYAFTSSAATSAALAARLEAGMLGINQTQVAWPELPFG
                     GVKDSGYGREGGSEAIQDYLVTKLITQAAG"
     gene            complement(9500..9772)
                     /locus_tag="VGB93_10900"
     CDS             complement(9500..9772)
                     /locus_tag="VGB93_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0146632.1"
                     /translation="MSGQATPRETLIDLVAAILKRNGATRPVDAEADLVNQGLTSVDM
                     VHLMLAIEAAFDITIPQSGLTPENFRSIATIEALLAKLTSPAEASS"
     gene            10018..>10984
                     /locus_tag="VGB93_10905"
     CDS             10018..>10984
                     /locus_tag="VGB93_10905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0146633.1"
                     /translation="MTVQNVHLREAQGEETATNARADLASRGARVVEVAAAHAASVDV
                     EARFPTAAIEAMREGGLLSLMVPQDLGGEGLPLSAIADVCYTLGQACSATAMIFAMHH
                     SAAACVIRHAGASAWHRDFLRQIANAQLLLASSTTEGQNGGNVRASAAAIEGSGDSIT
                     LDRAATVISYGAEADAIVTTARRADDAPSSDQILVTFVKGDYTLERLSGWNALGMRGT
                     ASAGFTLKARGDRAQILPEPYERVHAQTMQPVSHLLWASVWAGIAASAVGRAQAFIRK
                     AARQSGGALPPGAAHYTKARSTLQMLCALITSSLTRFEAVADDPKA"
BASE COUNT         2179 a         3223 c         3427 g         2155 t
ORIGIN      
        1 gccggtgaac gtgtcgccga cgaccgtgcc gctgtcgttg ttgcgcagaa agctcgcgtc
       61 gcgtcgcaaa taggagaatc ggttgttcaa tgtgagaaac ttgttgatcg tccacgcgtc
      121 gctcaaatcg gttctcaaat aatcttggtc gacgtaaccg aacggcgtcg aatatttcgc
      181 atcgcgcgaa acgtcgatcg ggctgccgtg gaaatagatc aggccgtagc tgtcgggcgt
      241 cccaatgata tgccgcgcat cgacggaaaa tgtgatgaaa tggtcgccga gattccaagt
      301 cagttccggc ctgatttcgt agtcgtcgct tttaagaccc ctgaagccgt cggaatgtgc
      361 cacggtcgca tcgacgcgat aactcaggcc gttcaccagc gtcggcccgg tcagccagta
      421 gttcgagttg atcgtattga acgatccata ttgggcgccg acgccgccag agaatgtgga
      481 tgaaggtagg taatgcgtga tattgatcga accgccgggc ggcccgctgc cgagcagggc
      541 cgatcccggc ccctttagcg cttcgatact ttgcacgccg ttgagagaat gcggcagacc
      601 gttgacctgt tcgccgtcgg agaaaccatc ctcgtagatc cgcgcgtcga gaccgcggat
      661 caaaaaccgg tcgaagccgc cgatcgaatc tggtccgccg gtgctcgtgc cgctaacgtc
      721 gcgcagcgcg tccttgacgt ccgtaccacc ctgttgcgtg acgagctggc tcggcacgac
      781 tttgacgctc aggggtaaat ccgagacctt agtccctgtc agcccgactt gtggaacctg
      841 ctggagaggc ggtgtcgatt ggatgttgtc ccctccgggt ggggcggtga catcgatatg
      901 gggtagggct tccgtcccgc cagcgctcgc ctgttgcgca ttgacggcac tgaattcgaa
      961 cgtcgatccg atcagcgcgc cgatgaagat cggtaccgcg atgaacgcgc gtcctcccat
     1021 tttcggttgc tgcagaaggc gcgtttcaag aagatgacgt ggtgggctct cggcgtatat
     1081 tcgagatatt ctcgatatag tctcgtggag tttgccgtaa tacgacgcca atgcacacat
     1141 catttctttg cagcccttcc aaaacacacg aacgccaatg ccgaagcgcg gtgagtaagg
     1201 ggctgataat tttgaaatac atgagcggtc aatcgaagta gtctataata cttataagac
     1261 tgacttctat gtcaggtttt gaatgactta aatcttgcga ggaagctttg cgtaatcagt
     1321 ggacaagcaa gaatttgagc gcggattcac gaggcccgaa ggaccgagtt tttacgatgt
     1381 ggcttcgaaa taaacgcccg cgccgttgcg gagcggtgac acgtctgctc agacagaacc
     1441 ggcgcgcgcg gcatcaatac ggttttgcgg aagacgcgtt gcccgagcga cgacgcgcag
     1501 caatgatggc aaccgttcca gggtcgccat gccggccttt tggccgagcg gttgcaatag
     1561 catttgcgaa agcaaggtcg cgcgctgcac ttgcggcgct acgtcctggg cgaggcgcgc
     1621 ttgaaacgtc cgcgcgtcat ggccttgcgc gtaatattgc gccgcgagcc gcgctgaatg
     1681 gagcgcgatc gacatgccat cgccggagaa agatggcgtg caggccgcct ggtcgccaag
     1741 ccgccagagt ccatcggcgc gacgctgcac gaaaccataa gggatcgcgc tgatcgctaa
     1801 agggcgcggc cagcacggct gtgcgccatc gagacgccgg cgcagatgcg ggcagttttt
     1861 cagcagcgac gcgaacagag ttggccagct ttgtccgcag gcaaatgtcc gtctgcgaac
     1921 cacgaggcaa agatttgcga catcgttctc gacggcttcg agtcccgcat atccgcctgg
     1981 aaaaagaaac agttcgaccg cgccccgcag ggcctgcgtt tgcgaggcgg aaagccgcca
     2041 gtgcaatttg aacccgatga gatcgttttg cgatccgggc ggccgcttcc aaccgcgcag
     2101 atcatgtttg cccgtcgcaa ggaatatttc agaggctgaa acgcttgttc catcatcgag
     2161 caaggcacgc caacgatgtc cgtctcgctc gaccccgatt gccttgtgtc cacgcagcac
     2221 ccgggcgccg caacgagccg ctctgttcag cagagcctcg tccaaggcat agcgcgaaag
     2281 gctccgcgcc gaaaacggca gatacgccgt ggcctgcctc tcgccggaga acatgccaac
     2341 gcgctcgatt gcaacggcgc ccaggccgtc gagatctatg ccgagatcac gcagatagag
     2401 catggcctcg gcgctgacga actcgccaca gaccttgtgt tgcccccgcg tttcacgctc
     2461 gacgagggtg acagcgcgcc cggcgttggc gaggctcacc gcaagccctg cgcccgcaag
     2521 gccgccgccg atgaccaggg ccggagtgct catttcggct tgatttgcga cacgcagagc
     2581 cggaatggca cgtggcctct gacgcgggcg cccgagatat cggcggcgcc aagaagttgt
     2641 tgccaatcgt ctgcgctgaa tgcccgtgcg attgagacag gtccgtcgtg ctggacgaaa
     2701 tgatgaaacc gcagcgcatg cgaggcgtgt ttgaaagcat gataggcgag aggatggcgc
     2761 tggagatcgt tgacgaacca gccgatccgt gtgttcgcct ccatccagca caggaatttc
     2821 acgatggcct cgttgtcaag gtgatgcgtg aagagcgaag agatgacgat atcgatgggc
     2881 gccgccggcc gataatcgaa aatattcgcc gtgacaaaac ggaggcgcgg attttcttgc
     2941 gtcaattgcg cggcggtctt cgccgaccac gggttgagat cgaccccaac gaggtcgaac
     3001 ttatagccct ctgctgtggc ccagaggtcg atccggcgca acatatcgcc cgacccgctc
     3061 cccacatcga cgacgcttac ggcgcggttg cgcggcaggg cgccgctcac cgcgagtcgc
     3121 ttgagaaacg acagtgttgg gcgataggca agcgtcagcc ggttgacctt ggtgagatcc
     3181 acgagacatt cgcgaaaggt cgcgaagtcc gtgtcctcgg cgtccatcag ctcggacaaa
     3241 tcattgcgtc gcgagaaatc gagcgcaatc atcacgctgc catccggaac agcatggttt
     3301 cggccgtgag gcccggacca aacgccagtg cgcagccgct ggcgccgcgc ttggtcttgt
     3361 ctcgcatgag acgctccagc acgaacaaaa tcgttgccga ggacatattg ccgtaatcgc
     3421 gcaagaccgc gcgcgaggcg gcgagcgccg aatcttccag ctcgaacgcc ccttcgaccg
     3481 catcgaggat cgaacggccg ccaggatgga cggcccagat gtcgatcgcc tccatcggct
     3541 tgccgccgag cacacgcgcc gagctttcgc gcagtgcgcc ggtgatggtg gagggaaccc
     3601 ggcccgacaa aaccatatcg aaaccggcgt cgcggatatt ccaggtgatt tgatcggctg
     3661 ctttctgcac aagctccgcg tggaaactgt cgagggcgat gccgtccggt gccgcactga
     3721 tcagcgcagc cgcacatccg tcaccaaata gcagaaatgt tagaagccgg tcgagatcgg
     3781 ctgtttcctg gaagtgcaat gtgcagagtt ctagacatac gaccagcaca ttcgcggttg
     3841 gcgtcgaacg cacgatgtgg tgcgcgattt tcaacgcgtt catggccgca tggcatccca
     3901 tgaacccgat gacggtcctc tcgacggacg aacttagccc gcaccgttcg agcagttcga
     3961 gatcgacacc cggcgccgaa aagccggtgc aacttgccac aatgaggtga gtgatgccct
     4021 ttcgctcact atcgtcgagg ccgagccgtt cgacggctgt agcgccgagc gcgggcgcgg
     4081 cgaacgcata gcgcgccata cggtcggcgg tggaggggaa atgtcctggc ttgtagaagc
     4141 cttcgccgtc gaggacctcg acgccgccat tcggcggcgc caggcatgac caacgacgct
     4201 cgatctgcgc ccggttcgcc atgcgttcaa acaaagcgcg cgacgtgcgg ttttgcagag
     4261 tctgccccgc gaagcgcacg aagtccgcgt gcacttcgtg cgcgggaaca gcggtcgcaa
     4321 cgcggttgat gtaagctatc gtcattggtc aattcctaat cgagaacgtc gaggtcaatt
     4381 gcagacccac gccggcgtcc aaccccaacg cgtccagact tcgcgataac aggcactatc
     4441 gtagctgctg tcgtcataat cgccgtcgta ccagaaggga tcgccgaaga agccgttgcg
     4501 tgcgaacccg cgaccgcgga aatggccatg aaaggaatgg ccatcatgaa aacccggacg
     4561 gccgatggct gcgaaatggc cgcctccagc gtgaccgaag ccaccgccag caaagccagc
     4621 gtggccaaag ctactgccgc cgaaacctcc gccgccgtgg aagcctccac cgccgaaacc
     4681 gccgccacca aaaccaccgc cgtggcctcc gccaccacca ccgccgccgt gcgcccacgc
     4741 ccccgacgcg gaaacggaaa gcgccgcggc tgctatcaat gccaatgccg acttgcgtaa
     4801 catggtgttc gctccatcac tcaaatccac cccaggaccc tcgtgggcgc cgttgccaga
     4861 ggcgacgaca ccgcgagccg gatgcttaag tcgcgatgca aaataggcaa atgactaatg
     4921 attcaatttc tgactcagat gtcagtttgc gagacggcga cctgtgctaa aatcggtttc
     4981 acgaattcga gagatttgga agcatggcgg agaaccggcg tcgcatattg atcattgaag
     5041 atgatcgcga gactgcagaa cagctcgtgg aatttgttga aacgagcggc tacgaagttg
     5101 acctcgcgtt gaacggcgaa gaagggttcg agcgtggccg ctcacccgac tacgctgtga
     5161 tggttattga ccggatgttg ccgagcatcg acggcatcgc gatcattcgc cgcctgcgcg
     5221 agattggcat cgtcacgccg acattgatca tcagcgcttt gggtgaggtc gacgataaag
     5281 tacgtggctt gcgtgccggt ggcgacgatt atctggtcaa gcctttcgct tttactgaat
     5341 tgttggcgcg cgtcgatgcg ctcgcgcggc gcagcacggc ggccgccaag gatctggtgc
     5401 tccgcgttgg cgatctcacg ctggatctcg tatcgcgcag cgtgacccgt gcgggccgcg
     5461 aaatcgaact gctgccacgc gaattccagg tgctcgaata tctcgtccgt aacgagggcc
     5521 acgttgtgcc ccgcgcgatg cttttgcagc atgtctggga tctgcatttc gatccgacga
     5581 cgaatatcat cgacgtctat gtcgggcgcg tacgccgtaa ggttgacagt ttgcgcgact
     5641 atcccttgat ccatacagtg cgcggcgtgg gattttgcgt tcgtgctcct agctaagaca
     5701 tttcgctcgc gcacattccg gatcgcgttg acggcgattg ccggtttcgg cacgatcgtt
     5761 ctctccctgc tcggctacat ctattggtca acgacgtctt acgttgcgag ccggtccgac
     5821 cgcgcgatcg cggtcgagca atcgcttctg caggcggcct atgaccaggg cggccgcggc
     5881 gcgctagagg cagcgatcaa gacccggatc gcggagaagg ctttcaatgg cggcctttat
     5941 ttgctcgcgg atcagaattt caaaccggtc gcaggcgatc tcgatagctg gccgcatgaa
     6001 ttgagcggcg cgtccggctt cggaaacttt ggcgcgccgg acacgggcgg ccgctccagt
     6061 tatcgcgctc tcatcgacac gttgcccgac aactcgcatc tgctcgtcgg gcgcaacgcg
     6121 gaagaccttg aatcgttcaa gcggacgatc gacaccgctt tggccttcac gcttttgctg
     6181 atcgccgtgc tcgcaggcgt cgtcagcatt cttgtcacgc gccggacagt cggccgcatc
     6241 gaatcgatca atgccacaag tcgcgccatc atggagcggg gtcttgcggc gcgtatcgga
     6301 ctgcgtgggg tcaacgacga atgggatgaa ctcgccagaa atcttaactc gatgctcgat
     6361 cgtatcgagg ccttgatgcg cgaggttcgt gaagttaccg acaatgtcgc gcatgatctt
     6421 cgcacgccgc tggcgcggat aagaggcagg ctcgagaagg cctcgggcag tccgcgcagt
     6481 gcggaatcgg atcagaaatt gatcgacgat atgatcggcg acctcgacga tgttctacga
     6541 atcttttcgt cgctgatgcg aatctcgcag atcgagtctg cggctcgcac agagggattc
     6601 gcggtggttg atctcgcggc ggtcgcgcgc gacgtcgtcg agcttttcga tgcggcggcg
     6661 gaggagaagc cgatcgaact atctgttgcc ggcgacccat gtgttgtggt caccggggat
     6721 cgtgacctcc tcttcgatgc ggtggccaat ctcgtcgata acgccatcaa atatgggaaa
     6781 aagaacgggc tggtgacggt gacggtgcgg gcgctcgaac atgatgcgct gatcgccgtc
     6841 gccgacgatg gaccgggcat cccgtccgac gaattcgcgc atattttcaa gcgcttctac
     6901 cggcttgagc gcagccggtg caccgctggc aatgggctcg gtctaagcct cgttgcggcc
     6961 gtcgcgcatc tgcatcgggc gcggattgat ctgaaagata atcagcctgg gttggcgttc
     7021 cggttgatta tccccagcgg agacgcggcc ttcggcgaga aagtgagtga aggcaacgcg
     7081 cagggctgat tggcgtccag ctaggtttca atttgcgaga ttgaccggtc cgcattccat
     7141 gcggcgatgg tgcgcagcag tggcttcata tggaaggcgg cttccggttg caccatttga
     7201 agaatgccat ggcggtggag ggcttcttcc acgattggac ctatcgaggc cacgctgacg
     7261 tgggagaatg cctctttcaa ctttggcccg agcccggact tctccgcgac atcgaagagg
     7321 cgctcgactt gtggcgtact ggtgaaggcg atgacgtcga tgtggcccgc ggccatctct
     7381 tcgattgcgc cgacaacctg cgccgtctcg gcctgcgacg catagcggta gggcgtgacc
     7441 ggcgtcaaca ccgccccgcg tgtttgcaag gttgcgagca gcgcgttggg gccgtcgcca
     7501 ggataaagct gaacgccgat cggccgtccg gcgatatctt caggcgccag cgcttcggcc
     7561 accccttccg aagtcggcgt cgctgcggcc agtgcgggcg cgagtccgat ttcgcgcagg
     7621 gcccgcgccg gcttcggccc acgggtaatt gagcggacat tgccaagcgc ggcgataaag
     7681 gccgtttcgc gctctgtgcg ttgcgcgatc gccagcagcc gccgcaaccc ttcgccggtc
     7741 agccagatga catcctcaaa gccgcccgcg atgagcttat cgatccaggc ttcggcttcg
     7801 cggctgtcct cgagatcgag gatttgcacc agcgggcaac ggatcgcagt tccgccttgc
     7861 tcctcgatca tgttcacgaa aaggttaagc tgtcggcttt cgggaatgag gatgcgaaga
     7921 ccgtcgagat tgcccatatc gtctctttga tgcgcggcgg tgcgccgctc tttccatccg
     7981 agctaagaga gccgcaattt attccagtgc gcaagagcga taagctcgac caccgcccca
     8041 gacgagagag ccgatgaccg agacctatcc cgatgtgcag cttctgattg acggaaaatg
     8101 gcggccgggc gccagcggcc agacgatccc cgtcatcaat ccggcgacgg aagaggtgat
     8161 cggtcaggtc gcacatgcgg acattgcgga cctcgatttg gcgctcgctg ccgcgaagag
     8221 aggtttcgcg gtttggcgga acgtctcggc gttcgagcgc ggcaagattt tgcgccgtgc
     8281 cggtgagctt ctgcgcgagc gcgccgagca cgttgcgctg ttgatgacgc gcgagcaggg
     8341 caagccgctg actgaagcgc gggtcgaagt catggccgcc gccgacacgc tcgactggtt
     8401 cggtgaagaa gcgcgccgcg cctatggccg catcgttccg gcgcgcgcgc cgaatgtcac
     8461 caatctcgtt atgaaggagc cggtcggccc cgtcgctgca ttcgcaccat ggaattttcc
     8521 gctcaatcag acggtccgca aactcggcgc ggcgcttgcg gcgggctgtt cgatcatcgt
     8581 gaaagcgccg gaagagacgc cagcgtcgcc agcggaattg atccgcgccg tcatcgatgc
     8641 gggcgtgccg gatggcgtcg tcaatctcgt ttacggtgtg ccggcggaaa tctccgaaca
     8701 tctcattcca catgaggcga tccggaaaat atcattcacc gggtccaccg ccgttggcaa
     8761 acagcttgcg gcgctcgccg gcaaacatat gaaacgcatc acgatggaac tcggcggcca
     8821 tgcgcctgct attgtcttcg acgacgcgga tgtcgatgcg gcggccaagc ttctcgtcgc
     8881 cgcgaaatat cgcaacggcg gccaggtctg caccgcgcca acgcgcttcc ttgtgcaacg
     8941 gaaagtctac gaaccgtttc tggatcgctt cgttgcggcc gcacaatcgt tgaaagtcgg
     9001 taatggcacg gcgccagata cgcagcttgg tcccttggcc aatccccgtc gcattgatgc
     9061 aatggaagcc ctgatcgcgg acgcgctcgg caagggcgcg gagttgcgcg ccggcggcgc
     9121 gcgccttggc aacaaaggtt acttcttcgc ggcgaccgtg ctggtaaatg tgccgttgac
     9181 ggcacgcgcg atgaacgatg agcctttcgg gccgctcgcg ctcgtcaatg cgttcgatga
     9241 tctcgacgag gctttggcgg agggcaatcg gttgccctat gcgcttgcgt cctatgcttt
     9301 cacaagttcc gccgcgacga gcgcagcttt ggcggcgcgc cttgaggccg gcatgctcgg
     9361 catcaaccag acgcaggtcg cttggccgga attgcccttc ggcggtgtca aggatagtgg
     9421 ttacggtcgt gaaggcggca gcgaagccat ccaggattat ctcgtgacaa agctcatcac
     9481 gcaggcggcg ggttaatttt caggacgaag cttccgcggg cgaagtgagc ttggcgagaa
     9541 gggcttcgat ggttgcgatc gagcggaaat tttccggcgt cagccccgat tgcgggatcg
     9601 tgatgtcaaa agcggcttcg atcgcgagca tcagatgtac catgtcgacg gaggtgaggc
     9661 cctgattgac aagatcggcc tcagcgtcaa ccggccgcgt ggcgccgttt ctcttcaaaa
     9721 tagcggcgac gaggtctatc aacgtctctc gcggtgtggc ctgtcctgac atcaaggtct
     9781 ccgtagaaat tatctcgaag ttttagcgaa gaaaaacgca gatacaaata aaatcccggg
     9841 cattaaccat aaatggttac aattggtcca gcaattgccg gcgggttagt taactaaggc
     9901 tcaagtaaca ggaattcgtg aagagcggaa ttaatggctg aattgagtat ttccgctaaa
     9961 tgagacgaat tacgtttctc attcgtgtgc gcggccgctg gcggactgga tttctggatg
    10021 acagtgcaaa acgttcatct gcgcgaggcg caaggcgagg aaaccgcgac gaatgctcgc
    10081 gctgatctgg catcgcgtgg cgcgcgtgtt gtggaagttg ccgcggcgca cgctgcttcg
    10141 gtggatgttg aagcgcgttt tccaaccgcc gcgattgagg ccatgcgcga gggcggtttg
    10201 ctgagcctga tggtgccgca ggatctcggc ggcgagggct tgccacttag cgccatcgcc
    10261 gatgtttgtt atacgctcgg gcaggcgtgt tctgccaccg cgatgatctt tgccatgcat
    10321 cacagcgcgg cggcctgcgt gatccgccat gccggcgcca gcgcctggca cagggacttt
    10381 ctgcggcaga tcgccaacgc gcaattgctc ctcgcgtcct cgacgacgga aggccaaaat
    10441 ggtggcaatg tccgcgccag tgcggcagca atagaaggga gcggggattc aatcacgctc
    10501 gatcgcgcgg cgaccgtcat ttcttatggc gccgaagccg atgcgatcgt gacgacggcg
    10561 cggcgcgccg acgatgcgcc aagctcggat cagattctcg tgacatttgt taaaggcgat
    10621 tacacgcttg agcggctgtc ggggtggaat gcgctgggga tgcgtgggac ggccagcgcc
    10681 ggcttcacgc tcaaggcgcg cggtgatcgc gcgcaaattc tgccggagcc ttacgaacgg
    10741 gtccatgcgc agacgatgca acccgtgtcg catttgctct gggccagcgt ctgggctggc
    10801 attgccgcga gcgctgtggg ccgcgcgcaa gcgttcattc gtaaagccgc gcggcaatcg
    10861 gggggcgcgt tgccgccggg tgcggcgcat tacaccaagg cgcgttcgac gctgcagatg
    10921 ctgtgcgcgc tgatcacatc gagtttaacg cgttttgaag ccgtggccga cgacccgaag
    10981 gcgc
//
LOCUS       DASWOG010000530         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_196929,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000530 DASWOG010000000
VERSION     DASWOG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196929"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGB93_10910"
     CDS             <1..213
                     /locus_tag="VGB93_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter permease"
                     /protein_id="HEY0146634.1"
                     /translation="NASIVSEVSSWGQDHLYAHGLGAYISQATAAGDYPRIVLGIVTM
                     SAFVVLFNRLLWRPLYGFSERRLRLN"
     gene            225..1556
                     /locus_tag="VGB93_10915"
     CDS             225..1556
                     /locus_tag="VGB93_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0146635.1"
                     /translation="MPEPEKIESAAADVELEIRHVRQTYARPSGAPLLVLDDINLTLH
                     TGEIVALLGRSGCGKSTLLRVVSGLAPSVAGEVRYHDRVVDGPAEGIAIVFQSFALFP
                     WLTVLQNVQAGLDARGVPEKAARTRALDAIELIGLDGFENAYPHELSGGMSQRVGFAR
                     ALVVDPTVLLMDEPFSALDVLTGEILRSDFMDLWTAERLSIKSVLLVTHNIEEAVFMA
                     DRILILAPNPGRIAAEIDVPLPRPRQRLSSEFRDLVDDIYGRMTAGPAPTADGKRPAL
                     ASIGTNLPIMSAVRIVGLADVLASAPFCGKADLQVLAARLHLSANDLFHIAEMNRMMG
                     FAELRDGDLHLTAAGRAIAQADIPERKRLFAEHLLRAIPLAAHIRRVLDERPEHIAPR
                     SRFIAELEDHLSTEDAESTLNAVIGWGRYAECFAYDTLHLRFSLEDSAAAE"
     gene            complement(1573..2022)
                     /locus_tag="VGB93_10920"
     CDS             complement(1573..2022)
                     /locus_tag="VGB93_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY0146636.1"
                     /translation="MGNLRIPNESWVLVCDGQKALLLRNEGDPDLLNLKPIEIHLEPH
                     PRTAELGTDAPGRSFQSHGAGRSAYEGTDFHAQEEAQFLQKITQIIDAAASERKFKHL
                     VLVAPPHALGLIRQNLTPAARALIFAEVHKDLTNVPIGTIEEHLASA"
     gene            2180..4096
                     /locus_tag="VGB93_10925"
     CDS             2180..4096
                     /locus_tag="VGB93_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146637.1"
                     /translation="MKSEIVEWLGQSDLLLPQLIAEGLAANDRVKARLSVLQMAAQHA
                     RDPQTAFDLTEECRAAGLDPVPLQALVKSASLSSGEIITAVGLAALGKAICDDMTTMS
                     QAVHAGDAARAEQYEARLAAIKAAIPLGVSDDLGANQIIRLTAISAEDSLHRLIMDLH
                     RALNTLATTHAQELIAGAHAYGLTSDDKTAVEAFMRGVDATRKLKFGHPGLAATAMRS
                     GSRFTIQNDIGETDAHVIVIGVETDAVIVTYTDVHHARAKFFADLLRNFPVQWSGLHK
                     AASKLHDEEAFYLVTGRFAFHDAEARDHFLEQLGASLVFLIDWNKARKVLREFVANAD
                     AIRILDWAALQRVGHRGFIELGGAVLVTSAVRHAASARIGFGERLAEALGRESAIDFL
                     KAVLRISVEALEQGGSERLAKAGIEEALVAHLQRADTSLLAIVIRQAGLAREIASGVA
                     QFLDGRRRQQVPEDTRLARRASRIEEKGDRIALEARSKIARLGVDRGIERLVDLIEQT
                     IDELEEAAFVATLLPKDAAPEILDCLIELCGIVVAGIESAAIGTASATEMPEGQRVDF
                     EDTLAAVTRLIEDEHRADVAERVVTTKILTGAYDLKTSLSVLSVAHPLERATDQLARF
                     GHSLRDHILADLST"
     gene            4109..>4640
                     /locus_tag="VGB93_10930"
     CDS             4109..>4640
                     /locus_tag="VGB93_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0146638.1"
                     /translation="MTILFIGDGSATLCPADVVGGKAANLAHMAALGLPVPPAFILPV
                     ELCAAITKGEPGARKTMVEGLDTGIKFLERTTGRIFGDRRRPLLVSVRSGAARSMPGM
                     LDTVLDVGCVPAAVRGLIRMTGNPHFAFDCRRRFLESYGGVVLGIAAALFAAPIASLA
                     AAENEAQKHDLDREALA"
BASE COUNT          853 a         1346 c         1426 g         1015 t
ORIGIN      
        1 aatgcgagca tcgtatcgga ggtttcctcc tggggacagg atcatctcta tgcccacggc
       61 ctgggcgcct atatcagcca ggcgaccgct gccggcgatt atccgcgcat cgtgctcggt
      121 atcgtaacaa tgtcggcttt cgtggttctc ttcaatcgcc tgctttggcg cccactttat
      181 ggctttagcg agcgccggct gcggctgaac tgacaggagg cattgtgccc gagcccgaaa
      241 aaatcgaaag cgccgctgcc gacgttgaac tcgaaatccg ccatgtgcgg cagacttatg
      301 cccgtccttc cggcgcgccg ctcctcgtcc tggacgatat caatctgact ctgcatacgg
      361 gcgagatcgt cgcgctgctc gggcgttcgg gctgcggaaa gtcaacgctg ctacgcgtcg
      421 tttcgggtct cgcgccgtcg gtcgccggag aagtgcgcta tcatgaccgc gtcgttgatg
      481 gtccggcgga agggattgcg attgtcttcc agagcttcgc cttgtttccc tggctcacgg
      541 tgctgcaaaa tgtccaggct ggcctcgatg cgcgtggcgt gccggaaaag gcggcgcgta
      601 cccgtgccct ggacgcgatc gaactcatcg gcctagacgg atttgaaaat gcctatccgc
      661 atgagctttc cggcggcatg agccagcgcg tcggttttgc gcgcgcactc gttgtcgatc
      721 cgacagttct cctgatggac gagccgttct cggcgctgga cgtgctgacc ggcgaaattc
      781 tgcgcagcga ttttatggac ctttggaccg ccgagcggct ctcgatcaaa tcggttctgc
      841 tcgtgactca caatatcgag gaggcggtct tcatggccga ccggattctc atcttggcgc
      901 ccaatcccgg ccgcatcgcg gcggaaatcg atgttccgct gccgcggcca cgtcagcgtc
      961 tgagcagtga gttccgcgac ctcgttgacg acatctatgg acggatgacg gctggccccg
     1021 cgcccacggc tgacggcaaa cggccggcgc tggcaagtat cggcaccaat ctgccgatca
     1081 tgtcggccgt acgaattgtc ggactagccg atgtcctggc atcggcacca ttctgtggca
     1141 aagcagatct ccaagtgctc gcagcacgtt tgcatctcag cgccaacgat ctctttcaca
     1201 ttgcagaaat gaatcggatg atgggttttg ccgaattgcg tgacggtgac cttcacttga
     1261 cagccgccgg ccgcgccatt gcccaagccg atataccgga acgcaaacgc ctctttgccg
     1321 aacatctgct gcgcgcgatt ccgctggcgg cgcatattcg ccgcgtgctc gacgaacggc
     1381 cggagcatat cgcgccccgc agtcgcttca ttgccgagct tgaggaccat ttaagcaccg
     1441 aggacgccga gagcacgctc aatgccgtca tcggctgggg gcgttatgct gaatgcttcg
     1501 cctacgacac gctgcacctc cggttcagtc tggaagattc agcggcggcg gagtaggggt
     1561 ggccgcttca actcacgcgc tcgccaaatg ctcttcgatt gtcccgatcg gcacattcgt
     1621 cagatcctta tgaacttccg cgaaaatgag agcccgcgcc gcgggcgtca ggttctgacg
     1681 gatgagcccg agcgcatgtg gtggcgcgac aagtaccaag tgcttgaact ttctctcgct
     1741 tgcagcagcg tcgatgatct gtgtgatttt ctgcagaaat tgggcttctt cctgagcatg
     1801 aaaatcggtg ccctcgtatg cgcttcggcc ggcaccgtgt gattggaagg accggcccgg
     1861 ggcatcggtg ccgagttcgg ccgtccgtgg gtgcggctcc aaatggattt cgatcggctt
     1921 gaggttaagc aggtccgggt cgccctcgtt tcggagcaga agagcttttt ggccgtcaca
     1981 aaccaggacc cagctttcat ttggaattct gaggtttccc atttcttttc ctcgttgtta
     2041 cgccagagtc ggctttctgt tgcagaattc ggccccaccg aatttgcctt atgtcaaaga
     2101 agtataatcg aaatgaggaa agatgatcgg gctggcgttc ttggggtgac gcggcgaagg
     2161 tgcatggctg cgaggcatta tgaaatcgga gatcgttgaa tggctgggac aatccgattt
     2221 gcttttgcca cagctcattg ccgagggttt ggcggccaat gacagggtga aggcacggct
     2281 gagcgttctc cagatggccg ctcaacatgc gcgcgacccg caaacggcat ttgatctgac
     2341 tgaggagtgc agggctgcgg gacttgatcc ggttccgctg caggcgcttg tcaaatcggc
     2401 aagcctttcg agcggagaaa tcatcaccgc agttggcctt gcagcgctgg gtaaggcaat
     2461 ctgcgacgat atgacgacaa tgtcacaggc tgttcatgct ggagatgccg cacgcgccga
     2521 acagtacgag gcgcggctgg ctgcgatcaa ggctgcgatt ccgctgggcg tgtccgacga
     2581 cctcggtgcc aatcagatca tcaggctgac agcaatttcg gccgaggata gtctgcatcg
     2641 cctgatcatg gacttgcatc gtgcgctcaa cacgctcgcc acgacgcacg cgcaagaatt
     2701 gatcgccggg gcgcatgcct acggcctgac gtctgacgat aagacggcgg tcgaggcctt
     2761 catgcgcggc gtcgacgcaa cccggaaatt gaaatttggc catcccggcc tcgccgcgac
     2821 cgcgatgcgt tcaggcagcc gttttacgat ccagaacgat attggcgaaa ccgatgccca
     2881 tgtcatcgtg atcggcgtcg agaccgacgc cgtgattgtt acatatacgg acgtccacca
     2941 tgctcgcgcc aaattcttcg ccgatctcct gcgcaatttt ccggttcaat ggagcggact
     3001 tcataaagcc gcgtcgaaat tgcatgatga agaggccttt tatctcgtta ccggccgttt
     3061 tgcctttcac gacgccgaag cgcgtgatca ctttcttgag cagctcggcg cctcgctcgt
     3121 ctttctgatc gactggaata aggcgcggaa agtcctgcgt gagttcgttg cgaatgcgga
     3181 tgcgatccgc attctggact gggccgcgct tcagcgggtg ggccaccgtg gctttatcga
     3241 gcttggcggt gcagttctcg tcacttcagc ggtgcggcat gccgcatcgg cgcgaattgg
     3301 cttcggcgag cgtttggcgg aggcacttgg gcgtgagtcg gcaattgatt ttctcaaggc
     3361 ggtcttgcgg atttcggttg aggccctgga gcagggcggc tcggagcgct tggcgaaagc
     3421 cggcatcgaa gaggcccttg tcgcgcattt gcagcgagcc gacacgagcc tcttggcgat
     3481 cgtgattcgt caagcgggtc tcgcccgtga aattgcctcc ggggtcgcgc aatttcttga
     3541 tggccggcga cggcaacaag tgccggaaga tacgcggctg gcgcggcgcg cgagccgtat
     3601 cgaagaaaaa ggcgatcgca tcgctctgga ggcgcgcagc aagattgcgc gattgggcgt
     3661 cgatcgcggc atcgagcgcc tggtcgattt gatcgagcag acgatcgatg agcttgaaga
     3721 ggccgcgttt gtcgcaacac tccttccgaa ggatgccgcg ccggagattc ttgattgtct
     3781 gattgagctt tgcggaattg tcgttgcggg catcgagtcc gcggccatcg gtacggcatc
     3841 cgccaccgaa atgccggagg gacagcgcgt cgatttcgag gatacgctgg ccgcggtcac
     3901 cagattgatc gaagacgagc accgggccga tgtcgccgag cgggtggtga cgacgaaaat
     3961 cctgaccggc gcttatgatc tcaaaacaag cctttcggtc ttgagcgtcg cgcatccgct
     4021 tgagcgtgcg accgatcaac tggcacgttt cggtcattcc ttgcgcgatc acatcctcgc
     4081 cgacctttca acctgacggc aaactcacat gacaatcctt tttattggcg atggctcggc
     4141 aacgctctgt ccggcggatg tcgttggcgg caaagcggcc aatctggcgc atatggcggc
     4201 tctcggcctc ccggtgcccc cagccttcat tctgcctgtc gaactttgcg ccgcgatcac
     4261 caagggcgag ccgggcgcca ggaagacgat ggtggagggg ctcgacacgg gaatcaaatt
     4321 tctcgaacgc acgaccggaa ggattttcgg cgaccggcgg cgcccccttt tggtgtccgt
     4381 gcgttcgggt gccgcgcgct caatgccggg catgttggat actgtcctcg atgtcggttg
     4441 cgtgcccgcc gccgttcgcg gcctgatccg catgaccgga aatccgcatt tcgcttttga
     4501 ttgccggcgc cgctttcttg aaagctatgg cggcgtggtt ctcggtattg cagctgcgct
     4561 cttcgccgca ccgatcgcat cactagctgc cgctgagaat gaagcacaga agcacgatct
     4621 cgatagagag gcgctggcga
//
LOCUS       DASWOG010000531         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_105034,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000531 DASWOG010000000
VERSION     DASWOG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105034"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGB93_10935"
     CDS             complement(<1..382)
                     /locus_tag="VGB93_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0146639.1"
                     /translation="MSGESKPWRPKLILASASVRRLELLQQIGLDPDALIPADLDETP
                     NRGELPRVLAARLAAEKAEAAAKIAAQRPELEGAFLVAADTVVAVGRRILPKCEVVDE
                     ATQCLRLLSGRVHRVYTGVTLITPK"
     gene            complement(398..616)
                     /gene="infA"
                     /locus_tag="VGB93_10940"
     CDS             complement(398..616)
                     /gene="infA"
                     /locus_tag="VGB93_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0146640.1"
                     /translation="MSKEELLEFPGVITELLPNATFRVKLENEHEIIAHTAGKMRKNR
                     IRVLTGDKVLVEMTPYDLTKGRITYRFK"
     gene            complement(723..1565)
                     /locus_tag="VGB93_10945"
     CDS             complement(723..1565)
                     /locus_tag="VGB93_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEY0146641.1"
                     /translation="MSDPLPRRFSVHEREPEQGERRLGLVRGRRFRPVPLRIILPNLV
                     TLLALSMGLTGIRFAIEGQFQIAVLVVIIAAILDGLDGRLARALRGTSRFGAELDSLA
                     DFVDFGVAPGLILYLWNLHQLKSFGWFAALVFAIACALRLARFNVSLDDPDRASWEGN
                     FFTGMPAPAGAILGLLPLYLNFSFLGLPATHVTAPFEIAYVLLIAFLMASRIPHFSAK
                     KVGRVPREYVIVVLFAIAALILLLASFPMEVLACLSLIYLATIPLAVMKYRAFAARDA
                     EAAT"
     gene            complement(1620..>2162)
                     /locus_tag="VGB93_10950"
     CDS             complement(1620..>2162)
                     /locus_tag="VGB93_10950"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY0146642.1"
                     /translation="FFRDPVRVMPQASDLVISPADGKVSAVGLAAPPPEMGLGADPLL
                     RISIFMSVFNVHVNRAPMAGRIAKIAYRPGIFLNADLDKASEDNERNGLVIDTAAGRI
                     GVVQIAGLIARRIVCFVEQGDSVDAGERFGLIRFGSRVDVYLPHGARALVAEGATAIA
                     GETILADFHADEPARLFKLA"
BASE COUNT          471 a          675 c          664 g          352 t
ORIGIN      
        1 ccttcggcgt gatcagcgtc acgccggtat aaacgcggtg tacgcggcct gagagtagcc
       61 gaaggcattg cgtcgcctca tcgacgacct cgcatttcgg cagaatgcgg cgcccgacgg
      121 caaccacagt atcggccgcg accagaaaag cgccctcaag ctcgggccgc tgggcggcga
      181 ttttcgccgc agcctccgcc ttttcggccg caagccgcgc ggcgaggaca cgcggcaact
      241 cgccacgatt gggcgtctca tcgagatcgg caggaatcag ggcgtcggga tcaagcccga
      301 tctgctgcaa gagttcgagc cggcgcacag acgccgaggc gaggatcagc ttgggccgcc
      361 agggtttgct ctcgccgctc acggccgccg cccgaattta cttgaaacga taggtaatgc
      421 gacccttggt caggtcgtaa ggggtcatct cgacgaggac tttgtcgccg gtcaagacgc
      481 ggatgcggtt cttgcgcatc tttccggccg tatgggcaat aatttcgtgc tcattctcga
      541 gtttgacccg gaaggtcgcg ttcgggagca attctgtgat tacgcccgga aactcgagca
      601 attcttcttt cgacatttga ttccttctgc tgaaaccggc cgccacggct tcatggatgt
      661 aggcggcgcg gaccctaatc gagaacgacg gatttgtgaa ctgccgcggc aacgccgtcc
      721 gctcaagtgg cggcctctgc gtcccgcgcg gcgaaagccc ggtatttcat gaccgccagc
      781 gggatggtcg cgagataaat caggcttagg caggccagaa cttccattgg aaagctcgcc
      841 agcagcaata tcaaagctgc gatggcgaac agaacaacaa tgacatattc gcgcggcacg
      901 cggccgacct tcttcgccga aaaatgcgga atccggctcg ccatcaggaa ggcgatcagc
      961 agcacatagg caatttcgaa cggcgccgtt acatgtgtcg ccgggaggcc gaggaacgag
     1021 aaattcagat agagcggcaa gagccccaaa atcgcgccgg cgggggccgg cataccggtg
     1081 aaaaaattgc cttcccagct cgcgcggtcg ggatcgtcga gcgaaacatt gaagcgggcg
     1141 agccgcagcg cacaggcgat agcgaagacc agcgccgcaa accagccgaa gcttttgagc
     1201 tgatgcaggt tccagagata aaggatcaag cccggcgcga cgccgaaatc gacaaaatcg
     1261 gccagcgaat caagctctgc cccgaaacgg ctcgtgccgc gcaaagcccg cgcaaggcgc
     1321 ccgtcgagac cgtctagaat ggcggcgata atcacgacga gaaccgcgat ctggaattgc
     1381 ccctcgatgg caaaacggat gcccgtcagg cccatcgaca gcgccagcag cgtcacgagg
     1441 ttgggtagga tgatgcgtaa cggtaccggc cggaaacggc gcccgcgtac gagtccgagc
     1501 cggcgctcgc cctgttccgg ctcgcgttca tgcacagaaa atcgtcgcgg caaaggatcg
     1561 ctcatcgccg ccttcataaa gcaaattgtg gcacgacgga aacctgcttg atctcggccc
     1621 taggccaact tgaacaggcg cgccggttca tcggcgtgga aatcggccag gatggtctcg
     1681 cccgcaatcg cggtcgcgcc ttccgcgacc agcgcccgcg ccccatgcgg cagatagaca
     1741 tcgacccgcg acccaaagcg gatcaggccg aaacgctcac ccgcatcgac gctatcgcct
     1801 tgttcgacga agcaaacgat tcgccgggcg atgaggccgg caatctgaac gacgccaatc
     1861 cgccccgcgg ccgtgtcgat cacgaggccg ttgcgctcgt tgtcctcgct ggccttgtcg
     1921 agatcagcgt tgagaaagat acccggccga taagcgatct tggcgatgcg gcccgccatc
     1981 ggcgcgcgat tcacatgcac gttgaagacc gacataaaaa tcgagatgcg cagcaagggg
     2041 tcggcgccga ggcccatttc cggcggcggc gccgcgagac ccacggcgga aaccttgccg
     2101 tccgccggtg aaatgacgag atcgcttgcc tgcggcatca cccgcaccgg atcgcggaag
     2161 aa
//
LOCUS       DASWOG010000532         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_177784,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000532 DASWOG010000000
VERSION     DASWOG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177784"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VGB93_10955"
     CDS             complement(<1..482)
                     /locus_tag="VGB93_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated channel"
                     /protein_id="HEY0146643.1"
                     /translation="MRYLLPLAATAAVFAGMGGAQAQQALPQINVTATPNSNSPLAPS
                     QQVLVQTEALDAARANIFTPTGANAYEIDEKAIDDLPQAENTPLEKVLLQAPGVAQDS
                     AASGDFHIRNEHANVQFRINGILLPDGVSGFGQVIETSFISSLTLLDGALQAEYGLHT
                     A"
     gene            complement(810..1373)
                     /locus_tag="VGB93_10960"
     CDS             complement(810..1373)
                     /locus_tag="VGB93_10960"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0146644.1"
                     /translation="MSRIRTISILCALTQFVGGVAFAQNSKPAPVVSQPETTTATFGA
                     WTLRCERRADVAKGQKLCEVEESVVPKDQQNPIAHIGIVHPLDTAKGVLRVNVIFPPN
                     IFIPTAPSIRAKDGDPGVPLVWQRCFSGGCFAEAAIPPAEIKAWRAVEADTGRITFTE
                     AVGRNLAVQFSLRGFAQALDALEKVKS"
     gene            complement(1462..2163)
                     /locus_tag="VGB93_10965"
     CDS             complement(1462..2163)
                     /locus_tag="VGB93_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY0146645.1"
                     /translation="MAARTYPLRWQQIGLELPLDPQFSREDFLISESNADALRMIEAW
                     PRWPESVLLLIGERGSGKSHLAAIWAAQARAEIFSGASLRGADVAALAEMPALVIDAV
                     DEIGAAEAPLFHLLNLARQGGRSLLLTAQRRPDLWGLKTPDLLSRLRLAPVVELGAPD
                     DALLQSVLGKLFRDRQLLVDPAVIDFIALHIDRSLEVARAFVELVDRVALARGVAVTR
                     SLARELLIDATDERD"
     gene            complement(2164..>2518)
                     /locus_tag="VGB93_10970"
     CDS             complement(2164..>2518)
                     /locus_tag="VGB93_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0146646.1"
                     /translation="LVAAAIIAIVQGWPHWTLFAEAIGVVVVGQFLEGNVISPKLVGD
                     NVGLHPVWLMFALLAFGSLFGFTGLIIAVPVAAATGVIMRFAVQKYRESHLFMNLPQG
                     QTLILPEPVNAPKEH"
BASE COUNT          527 a          759 c          750 g          482 t
ORIGIN      
        1 gcggtgtgca gaccgtattc tgcctgcaac gcgccatcga gcaatgtgag cgagctgatg
       61 aagctcgttt caatcacctg gccgaagcct gacacgccgt ccggcaacag aatgccgttg
      121 atgcggaatt gcacgttcgc gtgttcgtta cgaatgtgga aatcgccgct tgcggctgaa
      181 tcctgcgcca cgccgggggc ctgcaagagc accttttcga gcggcgtatt ttcggcctgc
      241 ggcaggtcgt cgatcgcctt ttcatcgatt tcgtaagcgt tggcccccgt gggtgtgaag
      301 atattcgcgc gcgcggcgtc gagcgcctca gtttgaacga gcacctgctg gctcggcgcc
      361 aaaggtgaat tgctgttcgg cgttgccgtc acgttgatct gcggaagcgc ttgctgcgcc
      421 tgtgctccgc ccatgccggc aaaaaccgcg gctgtcgccg caagtggaag aagataacgc
      481 accaagccgc cccgctatta ttagatatgt tatactgtta catatcgcgg gtgagaattg
      541 tcaactggtg ctgggacgat gtgacggcgt tgatcttggt cgttagcggc gtcggggcat
      601 cgcgttcatc ccggacgccg catccggcgc aacgcggaga accgccttga aaagcgatgt
      661 gttgataaat caggcgaggt tataccgggt ttatcgagga tcgcgtgaaa gccgcgatcc
      721 tgtccgatgc taacggatct agccgcggcc gcgcgccggc attttggggc gcgcaccgta
      781 agaagtgcgc gccgcttgag actttgaaac tacgacttta ccttctcgag cgcatcaagc
      841 gcctgcgcaa aaccgcgcag cgagaattgg acagccagat tgcggccgac cgcctcggtg
      901 aatgtgatgc gccccgtgtc ggcctcgacc gcacgccagg ccttgatctc cgcaggcggt
      961 atggccgcct cggcaaaaca gccgccggag aaacaccgct gccagacaag tggcacgccg
     1021 ggatcgccgt ctttcgcgcg gatgctgggg gcggtcggaa tgaagatatt cggcgggaaa
     1081 atcacattga cccgtagcac cccttttgcc gtatcgagcg gatgcacgat accgatatgg
     1141 gcgattgggt tttgctggtc tttgggaacg accgattcct cgacttcaca gagcttctga
     1201 cctttggcga catcggcacg gcgttcgcaa cgcagtgtcc aggcgccaaa tgtggcggtc
     1261 gtcgtttctg gctgagagac taccggcgcc ggcttgctat tttgggcgaa agcgacgccg
     1321 ccgacgaatt gtgtcaaagc gcacaaaatt gaaatcgttc taatacggct cacgatcata
     1381 ctcccgaaac tgcgccctgc ctttaggcgt cacgcaccgg ctggtcaaca taagtcagcg
     1441 agaaatattc cctatagccg tttagtcgcg ttcatccgtc gcatcgatga ggagttcgcg
     1501 cgccaaactt cgtgtcactg cgacgccgcg tgcaagcgcg acgcgatcga ccaactcgac
     1561 aaacgcgcgc gcaacctcga gcgaacggtc gatatgcaac gcgatgaaat cgatgacggc
     1621 cggatcgacc agcaattgtc gatcccggaa aagttttccc aggaccgatt gcagcaacgc
     1681 atcgtctggc gcgcccagtt cgacgaccgg cgcgagccgc aggcgcgaga gaagatcggg
     1741 cgtcttgagg ccccagagat cggggcgccg ctgcgccgtc aatagcaggc ttcgcccgcc
     1801 ctgccgcgca agattgagga gatgaaacaa gggcgcttcg gccgcgccga tttcatctac
     1861 ggcgtcgatc actagcgccg gcatttctgc cagcgccgca acgtctgcac ccctcagcga
     1921 cgctccggaa aaaatctccg ctcgcgcctg cgcggcccat attgccgcga gatgactttt
     1981 gccgctgccc cgctcgccga tgagcagcaa aacggactcc ggccaacgcg gccacgcctc
     2041 gatcatgcga agcgcgtcgg cattggactc cgagatcaga aaatcttcac gcgaaaattg
     2101 tggatcgagc ggcagttcga ggccgatctg ctgccagcgc aaaggatagg tccgcgccgc
     2161 cattcagtgc tctttgggcg cgtttacggg ctccggcagg ataagcgtct gcccttgtgg
     2221 cagattcatg aaaagatggc tttcgcgata tttctgcacg gcgaagcgca tgatgacgcc
     2281 ggtcgccgcg gccaccggca cggcgatgat gaggcccgtg aaaccgaaca gactgccgaa
     2341 ggccagcagg gcaaacatca gccagaccgg atgcagccca acattatcgc caacgagctt
     2401 gggcgaaata acatttccct cgagaaattg cccgacgacg acaacaccta tcgcctctgc
     2461 aaacagcgtc cagtgcggcc agccttgaac gatcgcaata atcgccgctg cgaccaaa
//
LOCUS       DASWOG010000533         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_325080,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000533 DASWOG010000000
VERSION     DASWOG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325080"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGB93_10975"
     CDS             complement(<1..543)
                     /locus_tag="VGB93_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0146647.1"
                     /translation="MNLVIVESPSKAKTINKYLGRDYEVIASFGHVRDLPPKDGSVDP
                     EQDFTMLWQVDAKAAKRLNDIAAAAKTANKLILATDPDREGEAISWHVLEVLKNKKVL
                     RDIPVERVVFNAITKTAVLDAMKHPRQIDAALVDSYLARRALDYLVGFNLSPVLWRKL
                     PGARSAGRVQSVALRIVSDRE"
     gene            806..1756
                     /locus_tag="VGB93_10980"
     CDS             806..1756
                     /locus_tag="VGB93_10980"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0146648.1"
                     /translation="MSAPQPRQKFRHRHLLGIEGLSAEDIMILLDRAEEAVEVSRQIE
                     KKRTTLRGRTLINLFFESSTRTQSSFELAGKRLGADVMNMSVQTSSLKKGETLIDTAV
                     TLNAMRPDLIVVRHAQAGAVHLLARKVDCAVINAGDGAHEHPTQALLDALTIRRNKGP
                     IEGLTVAICGDIMHSRVARSNIILLSALGARVRVVGPSTLMPVGIERMGVETFHDMKT
                     GLAGADIIMMLRLQRERMSGGFMPSSREYFRFFGLDQEKLRFAKPDALVMHPGPMNRG
                     VEIDSNIADGPQSLIREQVEMGVAVRMAVLAALAEHLPNE"
     gene            1763..>2766
                     /locus_tag="VGB93_10985"
     CDS             1763..>2766
                     /locus_tag="VGB93_10985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175815.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0146649.1"
                     /translation="MKHAPTGAIAHQPLALVNARLIDPARETEERGGVLIIDGIIRDL
                     GPQVVAASLPPHAHIIDCAGDVVAPGLIDMCAFVGEPGAEHRETIATATAAGAAGGVT
                     TILARPDTNPPIDEPAVVDFLQRRARDTGIVRVLPMAALTRGLHGEEIAEIGLLKEAG
                     AVAFSDGLQSLRNARVMRRVMAYAKDFDALIIHFAQDPDLATDGVMNEGELATRLGLP
                     GVPREAEAIMLDRDIRLVALTGARYHAALISTTLSLDAIRAAKAAGLPITCGTSINHL
                     ALNENDIGDYRTFLKLSPPLRREDERLALVEALAEGLIDVVVSDHNPQDVETKRLPFA
                     "
BASE COUNT          506 a          874 c          867 g          519 t
ORIGIN      
        1 ctcgcggtcg gagacgatgc gcaaagcgac ggattgcacg cggcccgccg agcgcgcgcc
       61 cggcaatttg cgccagagca ccggcgagag attgaagccg acaagataat cgagcgcccg
      121 gcgggcgagg tacgaatcga ccagcgccgc atcgatctgg cgcggatgct tcatcgcgtc
      181 gagcacggcg gtcttggtga tcgcattgaa cacgacacgc tcgaccggta tgtccctcaa
      241 gaccttcttg ttcttgagaa cttcgagaac atgccaggag atcgcttcgc cttcgcggtc
      301 cggatcggtc gccaggatca gcttattggc cgtcttcgcg gcggcggcaa tgtcgttgag
      361 ccgcttggcg gccttggcgt cgacctgcca aagcatagtg aaatcctgct ccggatcgac
      421 ggacccatcc ttgggcggta gatcgcgcac atggccgaac gaggcgatga cctcatagtc
      481 gcgcccaagg tacttattga tcgtcttggc cttggagggc gattcgacga tgacgagatt
      541 cataagttct cagctccgtg ctaaacggct gaagacgcta cattttttgg aaatcggcca
      601 agccgtgagg agctcccggc gcgtctctgg gccgccgctc attgtggcgg aacatggggt
      661 aggggaggcg tggtgtcaaa tcccggcccc aagcccggct cgcacatccg cctccaaatc
      721 ccggtaacca gtgcatcgaa agggccggga aatcctcgcg cgcggcttgc cgacgccgca
      781 ggttcacccg tatagaacca ctttaatgtc cgctccccag ccccgccaaa aatttcggca
      841 ccggcatttg ctcgggatcg aaggactttc agccgaagac attatgattc tcctcgaccg
      901 cgccgaagaa gcggtcgagg tctcgcggca gattgagaaa aagcgcacga cgctccgcgg
      961 ccgaaccctg atcaatctct tctttgaatc ttcgacgcgc acgcaatcgt cttttgagct
     1021 cgccggcaaa aggctcggcg ccgatgtgat gaacatgtcg gtccagacct cgagcctcaa
     1081 gaaaggcgag acgctgatcg ataccgctgt cacgctcaat gcaatgcgcc cggatcttat
     1141 cgtcgtgcgc catgcccagg cgggcgcggt acatcttctc gcccgcaagg tcgattgtgc
     1201 ggtgatcaat gccggcgacg gtgcgcatga gcacccgacc caagctctcc tcgatgcgct
     1261 gacgatccgc cgcaacaagg gcccgatcga aggcctgacg gtcgcaatct gcggcgacat
     1321 catgcattcg cgcgtcgcgc gctcgaatat cattctgcta tcggcgctgg gcgcacgggt
     1381 gcgcgttgtc ggcccctcga cgctgatgcc ggtcggtatt gagcgtatgg gcgtcgagac
     1441 ctttcacgac atgaaaaccg gacttgccgg tgccgacatc atcatgatgc tgcgcctgca
     1501 gcgcgagcgg atgagtggcg gcttcatgcc gtcgtcgcgc gaatatttcc gtttcttcgg
     1561 tctcgaccag gagaagctgc gcttcgccaa gccggacgcg ctagtcatgc atccggggcc
     1621 gatgaaccgc ggtgtcgaga tcgactcgaa tatcgccgac ggcccgcaga gcctgatccg
     1681 cgaacaggtc gaaatgggcg tggcggtccg catggccgtt ctcgcggcgc tggctgagca
     1741 tttgcccaat gagtgaggcg cgatgaaaca cgccccgacc ggcgccatcg cccaccagcc
     1801 gctggcgctc gtcaatgcgc ggctgatcga ccccgcgcgc gaaactgaag agcgcggcgg
     1861 cgtgctcatc atcgatggga tcattcgcga tctcgggccg caggtcgtcg ctgcgtcgct
     1921 gccgccgcat gcgcatatca tcgattgcgc cggcgatgtc gtcgcgccgg gcctcatcga
     1981 catgtgcgct tttgtcggcg agccgggtgc cgagcatcgc gagacgatcg cgaccgcgac
     2041 ggcggcgggg gcggcaggtg gagtcacgac cattctggcg cggcctgaca ccaatccgcc
     2101 gatcgacgaa ccggccgtgg tcgattttct ccaacggcgt gcccgcgata ccggcatcgt
     2161 ccgggttctg ccgatggccg ctttgacgcg cggcctacat ggcgaagaga tcgccgagat
     2221 cggccttttg aaagaggctg gcgcggtggc gttcagcgat gggctgcaat ctttacgcaa
     2281 cgcgcgcgtg atgcgccgtg tcatggccta tgccaaggat ttcgatgcgc tgatcattca
     2341 tttcgcgcaa gatcccgatc ttgccaccga cggcgtcatg aatgaaggcg agctggcgac
     2401 gcgccttggc ttgccgggtg tgccgcgcga ggcggaagcg atcatgctcg atcgcgacat
     2461 ccgcctcgtc gcgctgaccg gcgcgcgcta ccacgcggcg ctgatttcga cgaccctgtc
     2521 gctcgatgcc attcgcgccg ccaaggccgc gggcctgccg atcacctgcg gcacctcaat
     2581 caatcatctt gctctgaacg aaaatgatat cggcgattac cgcaccttcc tgaagctctc
     2641 gccgccgctg cggcgcgagg atgaacgcct ggcgctcgtt gaggcgcttg ccgaagggct
     2701 gatcgatgtc gtcgtctcgg accacaatcc gcaggacgtc gagacgaagc ggctgccgtt
     2761 tgcgga
//
LOCUS       DASWOG010000534         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_49122,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000534 DASWOG010000000
VERSION     DASWOG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49122"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGB93_10990"
     CDS             <1..256
                     /locus_tag="VGB93_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146650.1"
                     /translation="VGQSDNVGEGPDFSAQSNSGDLVIDRKKASDKFRNAESVAEIPC
                     LVYVGRKIIEASSCGVSGKVIPSGGRFNEWVFRPERYALV"
     gene            299..655
                     /locus_tag="VGB93_10995"
     CDS             299..655
                     /locus_tag="VGB93_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0146651.1"
                     /translation="MIEAVPVAERLEGAPVRLHRRWSDELKEQVVAESFLPGANVSAI
                     ARRLGIDPSQLFSWRKALRKGRVTMAASQPDALTEDKASSRPVIEIVVGDVVIRADAM
                     ADEAHLRRVIRAIRSA"
     gene            complement(721..1071)
                     /locus_tag="VGB93_11000"
     CDS             complement(721..1071)
                     /locus_tag="VGB93_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146652.1"
                     /translation="MTQIRAYFHPDGAHFRPRDSLCRSDLPNKLRRLRCHRMNPLPIH
                     AVDERQQLRLIELNPAMPDLRPAKLRFLQALRVKTNPRSIPKYDFDSVGPLGAKNVKR
                     TVKGIAAGVPHQRQ"
     gene            1070..>1551
                     /locus_tag="VGB93_11005"
     CDS             1070..>1551
                     /locus_tag="VGB93_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0146653.1"
                     /translation="MPAPVFDLPDNVDALKAMVLAMAEKAARAEVLESEVADLKALNA
                     SAEERIARLTSILKTLERARFGRRSEKLGSNALDDEQSAFVFDEVQTGLGAIQAELDK
                     RQDSDRGKRAARPRKGFAPHLERVEIVIEPVELPEHAGKPKILIGEDVSERLDVVAAK
                     "
BASE COUNT          308 a          400 c          500 g          343 t
ORIGIN      
        1 tgtaggacaa tccgacaatg ttggggaagg tccagacttt tccgcccaat ccaatagtgg
       61 tgatctcgtc attgaccgga aaaaagcctc cgacaaattc cgaaatgccg aatccgttgc
      121 cgagattcca tgcctcgtct acgtaggccg aaagattata gaagccagta gctgcggagt
      181 aagcggcaag gtgatcccat ctggcggtcg gtttaacgag tgggtattca gaccggagcg
      241 atatgctctg gtgtgatctt ctatgtctac tcctaggtct ggtgatagaa cattccatat
      301 gatcgaggcg gttccggttg cggaacgtct tgagggtgct ccggttcgtc tgcaccggcg
      361 gtggtcagat gagctcaaag agcaggtggt ggcggaatct tttctgcctg gggcgaatgt
      421 ttctgcgatt gcgcgtcgcc tcggcattga cccgtcgcag cttttttcct ggcgtaaggc
      481 gcttcgcaaa ggccgcgtga cgatggccgc gtcgcagccc gatgcactga ccgaagataa
      541 ggcgagttca cggccggtca tcgaaatcgt tgtcggcgat gttgtcatcc gtgcggatgc
      601 catggctgat gaggcccatt tacggcgcgt gatccgcgcg attcgctcgg catgatcccg
      661 tccggcgtca aagtttttct ggccagtcat ccgatcgatt ttcggaaggg catggacgga
      721 ttattgtcgc tggtgcggga cgccggcagc gatcccttta acggtgcgct ttacgttttt
      781 cgcgccaagc gggccgacag aatcaaaatc gtattttggg atggatctgg ggtttgtctt
      841 tacgcgaagc gcctggagaa agcgcagttt tgctggccga agatcgggca tagccgggtt
      901 cagctcaatc aggcgcagct gttggcgctc gtcgacggca tggattggaa gcgggttcat
      961 gcggtgacag cgaagccgcc tgagtttgtt gggtagatca ctgcggcaga gtgaatcacg
     1021 cggccgaaaa tgcgcgccat cggggtgaaa atatgctctg atctgggtca tgccggcgcc
     1081 ggttttcgat ctccctgaca atgttgatgc gctcaaagcc atggtgctgg ccatggccga
     1141 gaaagcggca cgcgctgagg ttcttgagag tgaagttgcc gatctcaaag ccctgaatgc
     1201 gagtgccgag gagcggatcg cgcgcctgac ctcgatcctc aagacgttgg agcgggcaag
     1261 gttcgggcgc cgctccgaga aactcggctc gaatgctctt gatgatgaac agagtgcctt
     1321 cgtcttcgac gaggttcaaa cggggctcgg cgccatccag gccgaactcg ataaacggca
     1381 ggactctgac aggggcaaac gcgccgcacg gcctcgcaag ggctttgcgc cgcatctcga
     1441 gcgggtcgag atcgtcatcg agccggttga gctaccggag cacgccggca agccgaagat
     1501 cctgatcggc gaggatgtct cggagcggct cgatgtggtc gcggcgaagt t
//
LOCUS       DASWOG010000535         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_342457,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000535 DASWOG010000000
VERSION     DASWOG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342457"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VGB93_11010"
     CDS             complement(<1..631)
                     /locus_tag="VGB93_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146654.1"
                     /translation="MHFDVIVVGGGHAGCEAAAAASRLGARTALVTHSFAAIGTMSCN
                     PAIGGLGKGHLVREIDALDGLMGRVADEAGIQFRVLNRTKGPAVRGPRAQMDRKLYRA
                     AMQTALNDVPGLTVVEGEAAEILTEAGRVTSLMLSDGRALKTGALVLTTGTFLRGMIH
                     VGAERVPAGRVGEPPAQQLSASLLAHGFALGRLKTGTPPRLDGTTIDWAM"
     gene            complement(671..1990)
                     /gene="mnmE"
                     /locus_tag="VGB93_11015"
     CDS             complement(671..1990)
                     /gene="mnmE"
                     /locus_tag="VGB93_11015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY0146655.1"
                     /translation="MSSASEAETIFALASGQGRAAIAVLRLSGAGVASILAAIVEKAT
                     PPRQAVLATFRDPVSGEAIDRGLCLFFPTPASFTGEDCAEFHLHGGPAVIAAMLAALA
                     FFPATRPAQAGEFARRAFENGKLDLTEIEGIADLIDAETEQQRRQALRQTRGDLRHKA
                     EGWRAALLEASALLEATIDFADEGDVSPLAQGRLAALLTSVRADLAAALARRRAGEIV
                     RDGVTVVIAGPPNAGKSTLLNALVQREAAIVSPIPGTTRDAIEVHLDIAGYAFVLIDT
                     AGLRETSDPLESIGIERARKRAEVADLVLWLDETGVTPADLPPGVPIWPIRSKSDLLE
                     GLCHNPLAISAATGANLDVLSERLADFARGVAGQAELGLITRERHRRAFLTAKAALDR
                     TSDAIDGPVELLSEDLRLAIHALESLIGKVDVEDVLGEIFASFCIGK"
     gene            2004..>3854
                     /locus_tag="VGB93_11020"
     CDS             2004..>3854
                     /locus_tag="VGB93_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0146656.1"
                     /translation="MSFNELANAASPYLLQHKDNPVHWRLWGPNTLADAQSANKPILL
                     SVGYAACHWCHVMAHESFEDNETAAVMNELFVNIKVDREERPDIDHIYMQALHAFGEQ
                     GGWPMTMFLTPKGEPFWGGTYFPKDSRYGRPAFVTVLRSVAKAFRDEQGKITSNAKAL
                     RLALGASEPSGQITPLSIATADALAPPLAQYVDPIDGGLRGAPKFPNPSILEFLWRAG
                     GRLGEKNYRDLVHLTLTKMSAGGIYDHLGGGYARYSTDDRWLVPHFEKMLYDNAQILE
                     LLALAWHAFGDDLFKQRAYETIGWLERELTRGGFCASLDADSEGEEGKFYIWTWDELV
                     EILGEADSEFFAAFYDATRAGNWSEGGRRVIILNQLQLKELPSTEEESRLAPLRQKLF
                     AVRETRVHPGLDDKIMADWNGLMIAALVNAATIFVEPRWLGLAARAYHYVVDHLQFND
                     NDGARRLAHSTRGGVVVTPGLALDHAAIARAALTLHEARHMEGAGLAAYDYLADAIAW
                     AEAIARHHVVAESSLVTMSADDANDIILRLTPTADDAIPNAHSVYLGALARLAAATGD
                     EKWRTRADALFAAVAAATNANPIGHTGILNALDLYLRGREIVTAGPERAPL"
BASE COUNT          741 a         1268 c         1146 g          699 t
ORIGIN      
        1 gcatcgccca atcgatggtc gtgccgtcga gacgcggcgg cgtcccggtc ttcagacgtc
       61 cgagcgcaaa cccatgcgcc aaaagactcg cggaaagctg ctgagccggc ggttcgccaa
      121 cgcgtcccgc aggaactcgc tctgcgccca cgtggatcat gccgcgcaga aatgtgccgg
      181 tcgtcagcac gagggcgccg gtcttaagtg cgcgcccgtc cgagagcatc aggcttgtca
      241 cgcgtcccgc ttcggtgagg atttctgccg cctcgccttc gaccacggtt agaccgggaa
      301 cgtcattcaa ggccgtctgc atagcggcgc gatacagctt gcgatccatt tgagcgcgcg
      361 ggccgcgcac cgccggcccc ttggtcctgt tcaagacacg gaattgaatg ccggcttcgt
      421 cggcgacgcg gcccatcagc ccatcgagcg catcgatctc gcggacgaga tgccccttgc
      481 cgaggccacc aatcgccgga ttgcaggaca tcgtgccgat ggcggcaaag ctgtgcgtca
      541 ccagcgccgt gcgggcgccc aaacgcgacg cggcggccgc ggcttcgcag ccggcatggc
      601 cgccgcccac cacgatcacg tcgaaatgca ttccgctcac gaatccgtcc aatgtttcac
      661 gtgaaacata tcatttaccg atgcagaagc tggcgaaaat ttcgccaagc acgtcttcga
      721 catcaacctt accaattaag ctctcgaggg cgtgaatcgc gagccgcagg tcctcactca
      781 gcagctcgac cggcccgtcg atggcgtcgg acgtcctgtc cagcgccgcc ttggccgtca
      841 ggaaggcgcg gcggtgacgt tcgcgcgtga tcaggcccaa ttccgcctgc ccagcaacgc
      901 cgcgggcgaa atccgccaac ctttcactca agacgtcgag gtttgcaccc gtcgctgcac
      961 tgattgccaa aggattgtgg cagaggcctt ccaaaagatc gctcttgctc cgaattggcc
     1021 agatcgggac gcccggcggc aaatcggctg gcgtcactcc cgtttcgtca agccaaagaa
     1081 ccagatccgc aacttcggcg cgtttgcgcg cacgctcgat gccgatgctt tcgagcggat
     1141 cggaggtttc ccgcaaacct gccgtgtcga tcagaacgaa ggcatagcca gcaatgtcga
     1201 gatggacttc gatcgcatcg cgtgtcgtgc cggggatagg cgaaacgatc gcggcctcac
     1261 gctgaacgag cgcattgagc agcgtggatt tgccggcatt gggcggcccc gcgatcacga
     1321 cggttacgcc gtcgcggacg atctccccgg ctcgcctcct tgccaaagcc gcggccagat
     1381 cggcccgcac cgaggtgagc aacgcggcga gccgcccctg agccaagggc gagacgtcgc
     1441 cctcatcggc aaagtcgatt gtggcttcca acaacgccga ggcttcgagt aaagcggcgc
     1501 gccagccttc ggccttgtgc cgcaaatcgc cacgcgtctg ccgcagcgcc tggcggcgct
     1561 gctgctcagt ctccgcgtcg atcaggtcgg cgatgccctc gatttcggtg agatcgagtt
     1621 tgccgttctc gaaggcgcgt cgagcgaatt cgccggcctg cgccggccgc gtcgcgggga
     1681 aaaacgccag cgcagcgagc atggccgcga tgacggcagg tccgccgtgg aggtggaatt
     1741 ccgcacaatc ctcgccggtg aaactcgccg gcgtcggaaa aaacagacag agtccgcggt
     1801 cgatggcttc ccccgacacc ggatcacgaa atgtcgcaag caccgcctgt cggggcggcg
     1861 tcgccttttc gacaatcgcc gctaatatgc tggcaacgcc ggcgcccgag aggcgtagaa
     1921 cggcaatcgc tgcccgcccc tgaccggatg cgagcgcaaa aatcgtttca gcctcgctag
     1981 cgctgctcat ctcaaggctc gccatgtcgt tcaacgaact cgccaacgcc gccagcccct
     2041 atctgctgca acataaggat aatcccgtcc attggcggct ctgggggccg aacacgctcg
     2101 ccgacgcgca gagcgccaat aagccgatcc tgctgtcggt cggctacgcc gcctgtcatt
     2161 ggtgccatgt gatggcccac gaaagtttcg aggacaacga aaccgccgcg gtcatgaacg
     2221 agcttttcgt gaacatcaag gtcgatcgcg aggaacggcc ggacatcgac cacatctata
     2281 tgcaggcgct ccatgccttt ggcgaacaag gcggctggcc gatgaccatg ttcctgactc
     2341 caaagggcga gccgttttgg ggtggcacct attttccgaa agactcacgc tatggccgtc
     2401 ccgccttcgt cacggtcctg cggagcgttg ccaaggcttt ccgcgacgag caaggtaaaa
     2461 ttacctcgaa cgcgaaggcc ctacgtctgg cgcttggagc ctcagaacca tcggggcaga
     2521 tcaccccgct cagcatcgcc accgccgatg cgctggcgcc gccgctcgcg caatatgtcg
     2581 atccgatcga cggcggccta cgcggtgcgc cgaaattccc caatccttcg atcctcgaat
     2641 ttttgtggcg cgctggcggt cgacttggcg agaagaatta tcgcgatctc gtccacctga
     2701 cgctgacgaa aatgtcggcg ggcggcatct acgatcattt gggcggcggc tatgcgcgct
     2761 attcgaccga cgaccgctgg ctcgtcccgc atttcgaaaa aatgctatac gacaatgcgc
     2821 agattctcga acttctggcg ctcgcctggc acgccttcgg cgacgatctc ttcaagcagc
     2881 gcgcttatga aacgatcggc tggcttgaac gcgaactgac acgcggcggc ttctgcgcct
     2941 cgctcgatgc cgatagcgaa ggcgaggaag gcaagttcta catctggaca tgggacgaac
     3001 tcgtcgaaat tctcggcgaa gcggattccg aattcttcgc cgctttttac gatgcaacgc
     3061 gtgccggcaa ttggtccgag ggcggccgtc gcgtcatcat cctcaatcaa ttgcagttga
     3121 aagaattgcc aagcaccgaa gaggaatcgc ggctcgcgcc actgcgacaa aaactttttg
     3181 ccgtgcgcga aacgcgcgtg catcccggcc tcgatgacaa gatcatggcc gattggaatg
     3241 ggctgatgat cgcggcgctc gtcaatgccg cgacgatctt tgtcgaacca cgctggctcg
     3301 gccttgcggc gcgcgcctat cattatgtcg tcgatcatct gcaattcaat gacaacgatg
     3361 gtgcacgccg cctcgcgcac agcacccgcg gcggcgttgt cgtgacgccc ggcctggcgc
     3421 tggatcatgc tgccatcgcg cgcgcggcgc tcaccttgca cgaagcacgg catatggaag
     3481 gcgccggcct tgcagcttac gattatctcg ccgacgccat cgcctgggca gaggcgatcg
     3541 cgcgtcatca tgtcgtcgcg gagagctccc ttgtcacgat gtcggccgac gacgcaaacg
     3601 atatcattct gcggctcacg ccaacagcgg atgatgcaat tcccaacgcg cattcggttt
     3661 atctcggcgc tttggcgcgg ctcgccgcag caaccggcga cgaaaaatgg cgcacgcggg
     3721 cagacgcgct ctttgccgcc gtcgcggctg ccacaaacgc caatccgatc ggtcataccg
     3781 gcattttgaa cgcgctcgat ctttatctac gcggccgcga aatcgtcact gccgggcccg
     3841 agcgcgcgcc gctt
//
LOCUS       DASWOG010000536         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_270194,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000536 DASWOG010000000
VERSION     DASWOG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270194"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGB93_11025"
     CDS             <1..198
                     /locus_tag="VGB93_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146657.1"
                     /translation="VGEIFAPYTGRALADAIERLLPRVTEFQSRAAHSVRDFAARHSG
                     DAYIDATLRFAEQREARRAKP"
     gene            455..1318
                     /locus_tag="VGB93_11030"
     CDS             455..1318
                     /locus_tag="VGB93_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146658.1"
                     /translation="MSEATVVSEIVQAKKYGIAIIANDRVIDWLLPFLESYKATSASI
                     PLYLIPYDDNLTRTRKAADAYDVEVVSLDSGELDALARRLYPLNPGHRRRLRKLLALA
                     LPLDEVIYLDVDTIMLQNFNKVLGYIEPGKTEFIVSSRCHDYIYNSRHKNYDFLRDAL
                     MFNDGFFVTSRHVLSLQDFYDVIAKDERTFHIVRQRGGLFAQPLTNFVVHRQNLKIAM
                     LPEIIPTASEESYYKAEGIAQGAGGPVDYKGEQLYFCHWPGIIGMPRRRRVVDQLWHQ
                     FADKAAARFSA"
     gene            complement(1308..2585)
                     /locus_tag="VGB93_11035"
     CDS             complement(1308..2585)
                     /locus_tag="VGB93_11035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0146659.1"
                     /translation="MNSAAPIAASFPLAGKTVLLVHPAWHSCGSYNVFMTQAQAYHAL
                     GAKVLSLAISDFPVNAAQSGAAKAYFDATQDLEVERRFFTGMPLGAVATRQFMRVVNA
                     WLHVDYAAMLIETARRSALPEELRATKIDLIHCNHFFCMPAAAKLRDEQGCPLLLETH
                     DLQARQFALRNRDAWTLSPKAKYETMLAQELAQMRVADVLIHLNAEEADAFGALLPEK
                     RHVLLFPAVAPMPAGAGDGIIIVASSNYPNYLGLKWFLSEVMPQAGDIPVRIVGNIYR
                     MVRWRAPLLYRRYKHLFTGFTADLGGVYAQAGTILLPTTSGFGISIKTIEALSCGVPL
                     VATRQAFRGMGLDPATLRNVTLAEDAIAFAEAMRQLTKTPNARDRVDSSSDTRQFYEK
                     LFAADAYRQRLAELVEPLLKNAADAPAKVVARR"
     gene            2964..>3229
                     /locus_tag="VGB93_11040"
     CDS             2964..>3229
                     /locus_tag="VGB93_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY0146660.1"
                     /translation="MMLPRLLYFYLGRRIFFSVVMLEVGLCVPVVMTQLFHSLPSSAV
                     RSGLLMPAFMGTFPTAVYLALPMAVGVDVALEFSRMAAEGMIAVL"
BASE COUNT          641 a          924 c          964 g          700 t
ORIGIN      
        1 gtcggggaaa ttttcgcgcc ctataccggc cgcgcgctgg cggatgcgat cgagcgcttg
       61 ctgccgcgcg tcaccgagtt tcaatcgcgc gcggcccata gcgtccgcga ctttgccgcg
      121 cgacacagtg gggatgccta tatcgatgcg acgctgcgat ttgccgagca gcgcgaggca
      181 aggagggcaa aaccttgatc cggcgtcccg ctgtcgctaa aaggaccaag ttcgtgatca
      241 tgcaaaggcc aagctatcgc gcgtaccttt tcccgcctta tccttttgga tcggcgttgt
      301 ttcataaaat ttgccgtctt ttcccggtgc atcggcgacg tgaactcgcg agcctgcacc
      361 gtgggcggtt cgtcggtttg gaatgtcgtg ggtccggggg gccgagccgg cgcattcgga
      421 attccggcgg atatcgtcgt cgcgaagtga gcccatgtcc gaagctaccg tagtgtcaga
      481 aatagttcag gctaaaaaat acggcatcgc catcatagca aatgatcgcg tcatcgactg
      541 gctgttgccg tttttagaaa gctacaaggc gacaagcgcg tcgattccgc tttatcttat
      601 tccatatgac gataacctca cgcgcacacg taaggctgca gacgcctatg acgtcgaagt
      661 cgtttccctc gacagcggcg aactcgacgc gctggcgcgg cggctctatc cgttgaatcc
      721 aggccaccgc cgccgattgc gtaaattgct cgccttggcg cttccgctcg acgaggtcat
      781 ttatctcgac gtcgacacga tcatgctcca gaatttcaat aaagtgctcg gatatatcga
      841 gccggggaaa actgagttta tcgtctcgtc tcggtgccac gattacatct acaattcacg
      901 gcacaaaaat tacgattttc tgcgtgacgc gcttatgttt aacgacggat tcttcgtgac
      961 gtcgcggcat gtgctttcgc tgcaggattt ttacgacgtc atagccaagg atgagcgcac
     1021 gtttcatatc gttcgtcagc ggggcggcct gttcgcccag ccgttgacca attttgtcgt
     1081 gcatcggcaa aatctgaaga tcgcgatgct accggagatc atcccgaccg cgtcggaaga
     1141 gagttactat aaggctgaag gaatcgcgca aggcgcgggg ggccccgtcg attacaaggg
     1201 cgagcagctg tacttctgcc attggccggg catcatcgga atgccgcgcc ggcgcagggt
     1261 ggtcgaccaa ttatggcatc aattcgccga caaggccgca gcccgcttca gcgcctagct
     1321 acgaccttgg ccggcgcgtc ggcggcattt ttcaaaagtg gttcgaccag ttctgcaagc
     1381 cgttgccgat aggcgtcggc tgcaaacagc ttttcgtaaa actgccgcgt atcgctgctg
     1441 ctatccactc tgtcgcgagc attgggcgtc tttgtgagct gccgcatcgc ctctgcgaag
     1501 gcgatcgcat cttcggccag tgtcacgttg cgcaaagtgg cgggatcaag tcccatgccg
     1561 cggaaggctt gccgcgtggc gacgagcggc acgccgcagg acagggcctc gatcgttttg
     1621 atcgaaatgc cgaaacccga tgttgtcggc aagaggatcg ttccagcctg cgcataaacg
     1681 ccgccgagat cggcggtaaa gccggtgaag agatgcttgt aacggcggta gagaagcggc
     1741 gcgcgccagc gaaccatgcg ataaatattg ccgacaatgc gcacggggat gtcgcccgcc
     1801 tgcggcatca cctcgctcaa aaaccatttg aggccgagat aattcggata attggaactc
     1861 gcgacaataa tgatgccgtc gcccgcgccg gcaggcatgg gcgcgacggc ggggaataac
     1921 aacacgtgcc gtttttccgg caaaagcgcg ccgaaggcgt ccgcttcttc cgcgttgaga
     1981 tggatgagaa catccgcgac acgcatttgc gcgagttcct gcgccagcat cgtctcgtat
     2041 ttcgccttcg gcgacagagt ccaagcgtcg cgattgcgca acgcgaattg tcgggcttgc
     2101 agatcgtgcg tttcaagcag gagcgggcag ccttgctcat cgcgcagctt tgccgccgcg
     2161 ggcatgcaaa agaaatggtt acaatggatg aggtcgattt tcgtcgcacg aagttcctcc
     2221 ggcagagcgg agcgccgcgc ggtttcgatc agcatcgccg cataatcgac atgcagccag
     2281 gcatttacga cccgcataaa ttgccgcgtg gccacagcgc caagaggcat gccagtgaaa
     2341 aagcgtcgct cgacctcgag gtcttgggtc gcatcgaaat aagccttcgc ggcgccggat
     2401 tgtgcggcgt tgaccggaaa gtccgagatc gccaggctca acactttggc gccaagtgcg
     2461 tggtaggctt gtgcctgcgt cataaaaaca ttgtagctgc cgcaggaatg ccaagccgga
     2521 tgcacaagca agaccgtctt gcccgcgagc gggaatgatg cggcgatggg agcggcggag
     2581 ttcatcgcgc gtagccctcg tccgcgttcg gatagcgatg atgtccttcg cctcgattgt
     2641 cacaatgtct cgcggtaagc cacgtaaccg aacccgaaac gacgcccggc gatcgaattt
     2701 gacacaaagg ccggctccga ttcgtgcggc cacccggcga ggggtgtttt tttgccggtc
     2761 ttctcgctat ctgcagtgag cttggtgttg cgtcggtcaa ggaaaggcct ggttgagcga
     2821 cgatgaacgc gcagctcatc gccgccaaag acgttatcgc ctttggacgc agaggtcgat
     2881 agtctcgccg tcgggcccgc aggcgaatcg cgtctcctgc gcgatgtata ctcgtcatag
     2941 gaacactggg aactcgacgc cggatgatgc taccgaggct gctctatttc tatcttggcc
     3001 gacggatatt cttctccgtc gtcatgctcg aagtgggcct ttgcgtgccg gtcgtgatga
     3061 ctcagctttt ccattctctg ccgtcgtcag cggttcgcag cggcctgctg atgcccgctt
     3121 tcatgggaac ttttccgacg gccgtttatc tcgccttgcc gatggcggtc ggcgttgatg
     3181 tcgcgctcga attttcacgc atggcggccg agggcatgat cgccgtgct
//
LOCUS       DASWOG010000537         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_197053,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000537 DASWOG010000000
VERSION     DASWOG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197053"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGB93_11045"
     CDS             complement(<1..473)
                     /locus_tag="VGB93_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146661.1"
                     /translation="MEKTATETNTLSPALIAIIAVSVGVLVANIYYAQPLIVSIGSSI
                     GVGTAFSGSLVTVTQIAYGAGLFFLVSLADIVENRRLVLSVMGVVILSLLGIAMSHSA
                     ATFFIASLFLGFCSPSVQILLPYVAHFTPPERRGRVVGIVMAGLLSGIMLARPAAL"
     gene            641..1372
                     /locus_tag="VGB93_11050"
     CDS             641..1372
                     /locus_tag="VGB93_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0146662.1"
                     /translation="MDCAPVIRTPGGNYVHNKYRGRQTMRIGIIGAGHIGSALARRLT
                     ELGHRIIIANSRGPATLAEVAAKTGATPVAVEDAVRGVDLIVLTIPMKNVSQLPSGLF
                     DSVPAATPVIDTCNYYPRQRDGRIAEIEDGLVESTWVARQIGHSVVKVFNSIYARHLA
                     DFGKESGSPGRIALPVAGDDPAAKAIVLTLVESLGFDALDAGPIAESWRQQPGTPAYC
                     TDLGVAALRKALADAPHERTANWRA"
     gene            complement(1376..2086)
                     /gene="bluB"
                     /locus_tag="VGB93_11055"
     CDS             complement(1376..2086)
                     /gene="bluB"
                     /locus_tag="VGB93_11055"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEY0146663.1"
                     /translation="MRLAEHGDDAADEADLAPAGAFSPEERAAVYRAIHARRDVRDEF
                     LPRPIPDATLTRILEAAHHAPSVGLSQPWNFVLIRDQATKTQVHDAFLRRNDEAAQMF
                     DAKRGARYRQLKLEGIRKAPLNICVTCDRARGGPVVLGKTHQPDTDLYSTVCAIQNLW
                     LAARAEGIGVGWVSIFDPNDLRPILGIPAEIAVVAYLCVGFVDQLFVRPELEARQWAE
                     RLPLDDLVFEGAWGKMRG"
     gene            2190..2663
                     /locus_tag="VGB93_11060"
     CDS             2190..2663
                     /locus_tag="VGB93_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146664.1"
                     /translation="MRLLLAAILALTVLPVRGDEVPFAQPASSLDQHHQSYTVALSDA
                     MELTQLRHIKLWLAGKSKNWPLTNYELAQLKDTFDKAAVLYLNIPVEYIAAVEKPLGT
                     LSGAVAAKDPVQFRRGFVALQAACNDCHKAADVGFIVIQTPTTSVFSDQNFAPGR"
BASE COUNT          556 a          822 c          820 g          519 t
ORIGIN      
        1 agcgcggccg ggcgtgccag catgatgccg ctcagcagcc cagccatgac aattccgaca
       61 acccgcccgc gccgttccgg tggcgtgaaa tgcgcgacat acggaaggag aatctgcacc
      121 gacggcgagc agaagccgag gaacaaagag gcgatgaaga aagtcgccgc cgaatgcgac
      181 attgcaatgc ccagcaggct gaggatcaca acgcccatca cgctcaacac aagccggcgg
      241 ttttcgacga tatccgcgag cgaaaccagg aagaacaggc cggcgccata ggcaatctgc
      301 gtcaccgtca cgagcgaacc ggagaacgcc gttccaacac cgatcgaact gccgatggaa
      361 acgattaacg gctgcgcata gtagatattc gcaacgagca cgccgaccga gaccgcgatg
      421 atcgcgatca gcgccggtga taaggtattt gtttctgtgg cagttttttc catggagtcc
      481 gcgcgaatat ccgtgacttg ccttgcgcgt cctcgctggt tcaatatgtt cttgcgcggt
      541 agctagtcgc gcgagaccct ggtgtctagg ggccctgtca cggccgcgct tgtcacattc
      601 gcggcaccta tgctgcggtc gcggtctgaa cagccaagtt atggattgcg ccccggtcat
      661 cagaacgccg ggcggcaatt atgtgcacaa taaatacagg gggagacaga cgatgaggat
      721 cggcatcatc ggcgcaggac atattggcag cgcgctggcg cgacgtctca ccgagctcgg
      781 tcatcgcatt atcattgcca attcgcgtgg tccggcaacg ctcgcagagg tcgcagcaaa
      841 gacgggcgca acgcctgttg cagttgaaga cgccgttcgc ggcgtcgatc tgatcgttct
      901 gacgatcccg atgaaaaacg tcagccagct ccccagcggt cttttcgact cggtgccggc
      961 tgcgactccg gtcatcgaca cgtgcaatta ttatccgcgt cagcgcgacg gccgtatcgc
     1021 cgagattgag gacggcttgg tggaaagcac atgggtcgca cgccagatcg gccatagcgt
     1081 ggtcaaagtg ttcaacagca tttatgcgcg gcatcttgcc gatttcggca aggaatccgg
     1141 ctcgccaggc cgcatcgcgt tgccggttgc cggtgacgat ccggccgcaa aagccatcgt
     1201 gctcaccttg gtcgaaagtc tcggcttcga cgcactcgat gccggtccga tcgcggagtc
     1261 ctggcgccag cagccgggga cgccggccta ttgtaccgat ctgggcgtcg ccgcattgcg
     1321 gaaggcgctg gccgatgcac cgcacgagcg cacggccaac tggcgcgcct aagcttcacc
     1381 cgcgcatttt gccccaagcg ccttcaaaga cgagatcgtc aagcggcaga cgctccgccc
     1441 attgacgcgc ctcaagctcc ggacgaacga aaagctgatc gacaaaaccg acgcagagat
     1501 aagccacgac ggcgatctcc gccggaatac cgaggatcgg acgcagatcg ttggggtcga
     1561 aaatgctgac ccagccgacg ccgatcccct cggcgcgcgc ggcaagccag agattctgga
     1621 tcgcgcagac ggtactgtaa aggtcggtgt ctggctgatg tgtcttgccg aggacgaccg
     1681 gcccgccccg cgcgcgatca caggtgacgc aaatgttcag tggcgctttg cggatgcctt
     1741 cgagcttgag ctgccggtaa cgcgcgccgc gcttcgcatc gaacatctgc gccgcctcgt
     1801 cgttgcggcg tagaaacgcg tcatgcactt gcgttttcgt cgcctgatcg cggatcagga
     1861 caaaattcca cggctggctg aggccgacag acggggcgtg atgcgcggct tcgagaatac
     1921 gcgtgagcgt cgcgtccgga attggccgcg gcagaaattc gtcgcgcaca tcgcgccggg
     1981 catggatggc gcgatagacg gccgcgcgct cctcaggcga gaaggcgcca gccggagcca
     2041 gatcggcctc gtcagctgca tcgtcgccgt gttcggcaag ccgcattgcg cctcctccct
     2101 gacattcggg cgcacctttg cattgttctt gatccggatc aaagcggatc ggcccctctg
     2161 tctgtgtaat taatctgacg gaggcattta tgcgattatt acttgcagct attcttgcac
     2221 tgaccgtgtt gccggtgcgc ggcgacgagg ttccgtttgc gcagcccgcg tcctcccttg
     2281 atcagcatca tcaatcctat accgtcgcgc tcagcgacgc gatggaactg actcagttgc
     2341 gccacatcaa gctctggctt gcgggcaaat cgaagaactg gccgttgacg aattacgaac
     2401 tcgcgcagct caaggataca ttcgacaagg cggcggttct ttatctcaat attccagtcg
     2461 aatatatcgc ggcggtggaa aagccgcttg gcactttaag cggtgcggtc gcggcgaagg
     2521 acccggtaca gtttcgacgc ggcttcgtgg cgctgcaagc ggcttgcaac gattgtcaca
     2581 aggcggccga cgttggtttc attgttattc agacgccaac aacatcggtc ttttccgacc
     2641 aaaatttcgc cccggggcgg tgatgaaaga cctgcctctc cctgaggtct ataaacttct
     2701 ggagcccggt ccggtcg
//
LOCUS       DASWOG010000538         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_270209,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000538 DASWOG010000000
VERSION     DASWOG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270209"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGB93_11065"
     CDS             <1..352
                     /locus_tag="VGB93_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0146665.1"
                     /translation="RIVNEKGRFTLVYLAAPDDAEAAKATKRPLIELTYNWDPETYTG
                     GRNFGHLAFAVDDIYATCEKLQKTGVTINRPPRDGYMAFIRSPDDISIELLQKGGAKP
                     VQEPWQSMPNTGVW"
     gene            complement(354..1076)
                     /locus_tag="VGB93_11070"
     CDS             complement(354..1076)
                     /locus_tag="VGB93_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0146666.1"
                     /translation="MTEAKLRHALVEASRELEKLQINQGTSGNLSVRSGDAMLITPSG
                     IPYDRLTPEMVALMPLSANDGAWRGPQKPSSEWRMHLDILRARPDDGAVIHTHSVYAT
                     ALSMLREEIPAAHYMVAAFGGPNVRCTPYAPYGTQELSDLVIEGLRDRHGVLLGQHGM
                     VVTGRDLGQAIWRAVELETLAKMYVIARSVGRPHILPDEEIAITVERFKSYGYQSDVV
                     AALVAAPRAAPTKKPAGKPRKL"
     gene            1720..2073
                     /locus_tag="VGB93_11075"
     CDS             1720..2073
                     /locus_tag="VGB93_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146667.1"
                     /translation="MSIDEIVHTCSNENVARAAVASIGVSFASRVRSAADLLGVSTGL
                     FAARAVRTFAAAAPIDEQREVVRAMFRADQPILRGLEAILERELDDVSSLDGRAWRPL
                     KPAMSSDAARDFCCP"
     gene            complement(2024..2533)
                     /locus_tag="VGB93_11080"
     CDS             complement(2024..2533)
                     /locus_tag="VGB93_11080"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY0146668.1"
                     /translation="MALYRDGRFESDSWRRLEADELPEEGHVLLSLAEWRRFKAEQKN
                     SNIAFGVVLEPGETAEMIAEDLPRLALVAVSFPKFTDGRGYSTARILRDRYKFAGELR
                     AVGDILFDQVQLYGRSGFDSLDIKDAITIKLLESGRKPVMTHFYQPAEGPEIKDSKSP
                     WRRRSTSRA"
     gene            complement(2536..>2731)
                     /locus_tag="VGB93_11085"
     CDS             complement(2536..>2731)
                     /locus_tag="VGB93_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY0146669.1"
                     /translation="KAYFKKYDLPPHPLVAKNYLSIGCIPCTSPVRPGEDARAGRWRG
                     RGKTECGVHIPATLEAGADI"
BASE COUNT          592 a          764 c          780 g          595 t
ORIGIN      
        1 ccgcatcgtt aacgagaagg gccgcttcac tctggtctat ctcgccgcgc ccgacgacgc
       61 ggaggcggcc aaggcaacca agcggccgct catcgaactc acctataatt gggacccgga
      121 aacttacacc ggcggccgca attttggcca tctcgcgttt gctgtcgatg atatctatgc
      181 gacctgcgaa aaattgcaga aaacgggcgt gacgatcaat cggccgccgc gcgatggata
      241 catggccttc atccgttcac cggacgacat ctcgatcgaa cttttgcaaa aaggcggcgc
      301 gaagcctgtg caagagccgt ggcagtccat gcccaacact ggcgtctggt agattagagc
      361 ttcctcggtt tgccggcggg ttttttcgtc ggcgcagcgc gcggcgcagc aacgagcgcc
      421 gcgacaacat cggattgata gccgtaggac ttgaaacgct cgaccgtgat cgcaatctcc
      481 tcatcgggca ggatgtgcgg gcggccgaca ctgcgggcga tcacgtacat tttcgccaat
      541 gtttcaagtt cgaccgcacg ccatatcgcc tgaccaagat cgcgcccggt cacgaccatg
      601 ccgtgctggc cgagtaaaac gccgtggcga tcacgcaatc cttcgatgac gagatcggac
      661 agttcctgtg tgccataagg cgcataaggt gtgcaccgca cattgggacc gccaaaggcg
      721 gcaaccatat aatgcgccgc gggaatttct tcgcgcaaca tcgaaagcgc tgtcgcgtag
      781 accgaatgtg tgtggatcac ggcaccgtca tcgggacggg cacgaagaat atcgagatgc
      841 atacgccatt cgctcgaagg cttttgcggg cctcgccagg cgccatcgtt tgccgaaagc
      901 ggcatgaggg cgaccatttc gggtgtcaaa cggtcgtagg gaatgccgct tggcgtgatc
      961 agcatcgcgt cgccggatcg gacagacaga ttgccggagg tgccctgatt gatttggagt
     1021 ttttccagct cgcgagaggc ctcaaccaga gcgtggcgaa gtttggcttc cgtcattgcg
     1081 tcgctgccgt tttaaaccgg atggaaaata tttctcgcaa gccggaaccc gatggatgtc
     1141 tgcgagttaa ggtaactgcg ctcggcttgg atgcctgtcc aaattgtgcg agcgcgatct
     1201 gccaagatga taatcgctcc agacaagaca taaacattta gtgcaatttc gcgctggttc
     1261 ttgggagatc attatcatta catcgcggcg cacacaaagc cgttcaaaaa ttcttcccca
     1321 taagcaaagt ttgactcgac aacgacgaag tttagcgcat tcttcgcgtg ctgtgccgat
     1381 acccgctgct cacttcaaat gttcgttgtg agcgagccca tctcaaactc tgagagtgct
     1441 cattccagca tggccaattt ttaatattgc atttggatga caacgcacaa cgccgtgagg
     1501 cggtaacacg aaagaatcgc gttaccgtca cgagtttgcc gagttcgacc gtcattggtt
     1561 ttttgtcgcc gccgatgacg aagatgcggc ctttgaaacg gcagcgatga actagattct
     1621 ttcaaattca gaaagactct aagtgtcgca aacgcgggag acatccatct aagccggatg
     1681 tctcatcgag tttgcaaaag caatgcaaag gaggcttcaa tgtcgatcga tgaaatcgtt
     1741 cacacatgct cgaacgagaa tgttgcgcga gcggctgtgg cttcgatcgg agtttccttt
     1801 gcttcgagag tccgatcggc tgccgacctt ttgggtgtga gcaccggcct attcgctgcg
     1861 cgggccgtac gtacgtttgc cgcggcggcg cctatcgacg agcagcgcga agtcgtgcgt
     1921 gcaatgtttc gcgccgacca gccaatcctg cgcgggcttg aggcgatcct ggaacgcgaa
     1981 ttggatgatg tcagttcgct cgatggtcgc gcgtggcggc cactcaagcc cgcgatgtcg
     2041 agcgacgccg ccagggactt ttgctgtcct tgatctctgg cccttcggcg ggctggtaga
     2101 aatgcgtcat caccggcttg cggccggatt cgagcaattt gatcgttatg gcgtctttga
     2161 tatcgagtga atcgaagcca gaacgaccat aaagctgaac ctgatcgaag aggatatcgc
     2221 cgacggcacg gagttcgccg gcgaatttgt agcggtcgcg caggattctc gccgtcgaat
     2281 aaccccgtcc gtcagtgaat ttggggaaac tcaccgcaac gagcgccagc cgtggcaaat
     2341 cctcggcaat catttctgcc gtttcgccgg gttcgagcac gacgccaaag gcgatattgc
     2401 tgtttttctg ttccgccttg aatcgccgcc attcggcgag gctcaacagc acatggccct
     2461 cttctggaag ttcgtcggcc tcaagccggc gccatgaatc gctctcgaac cttccgtcgc
     2521 gataaagcgc cataatcaga tatccgcacc ggcttcgaga gttgcgggaa tatgcacgcc
     2581 gcattcggtt ttgccacgac cgcgccagcg cccagcgcgc gcgtcttcgc cagggcgaac
     2641 cggcgatgtg cagggaatgc agccgatcga cagataattc ttcgcgacga gcgggtgcgg
     2701 cggcagatcg tacttcttga aataagcttt t
//
LOCUS       DASWOG010000539         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_122948,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000539 DASWOG010000000
VERSION     DASWOG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122948"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGB93_11090"
     CDS             <1..791
                     /locus_tag="VGB93_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0146670.1"
                     /translation="PDVTVPNQPPFGPNQPFLAGETFTRNDQPFDWSAGALYKLTPFL
                     SPYFGVSKSHLANFSAEAPAASVQAPEAALQYEAGVKGDALDNRLELTLSGFNVDRQN
                     VFTLVNDEANFSSQYTRGVDADLQFSVTPDWKIIANGTLQRAVLTANPSNPTAVGRVP
                     IGVPLQIANLWTTYNLRQLRLPDVTTGFGVEYRGKIFGDQLNTDEVPSYVIFDANITY
                     SQPKWDLGAGIKNIADTVYFTAANGAGAFVGDPRTFYVKGDVKF"
     gene            958..>1682
                     /locus_tag="VGB93_11095"
     CDS             958..>1682
                     /locus_tag="VGB93_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146671.1"
                     /translation="MTLAIDDSRRSIALWGGFAMMCVGMFMALLDTQVVVTSLPTIQR
                     ALGIPQDQMSWIQTAYLISEIISIPLTGLLTRVFGIRWLFIVSVSFFTLASIGCANSA
                     GFSSLILWRICQGFAGGTLIPAVFSAVFLLFPLHRQTLATMIASVLAVLAPTTGPLVG
                     GWVTQTYSWPWLFLINVIPGVLVAIAGIYLLPRASLRLDQLKDLDLISLQFGAGALAE
                     LEIAIKEAPGRGWISPWVLGLFA"
BASE COUNT          316 a          496 c          465 g          405 t
ORIGIN      
        1 cgccagacgt gacggtgcca aaccaaccgc ccttcggccc gaatcagccc tttcttgccg
       61 gcgaaacgtt cacgcgtaac gatcagccgt tcgattggag tgcgggcgcg ctttataagc
      121 tcacgccgtt cctttcgccc tatttcggcg tatcgaagag ccatctggcg aatttcagcg
      181 cggaggcgcc cgccgcctcc gttcaggcac cggaagcggc gctgcaatat gaagccgggg
      241 tcaagggcga tgcgctcgac aatcgattgg aactcacgct ttcgggattc aatgtcgatc
      301 ggcagaatgt cttcaccctc gtcaacgatg aggcaaattt cagcagccaa tacacccgcg
      361 gcgtcgatgc cgacctgcaa ttttccgtca cgcccgattg gaaaatcatt gccaacggga
      421 cgttgcagag ggccgtcctg actgcgaatc cgtcaaatcc tacggccgtc ggcagagtgc
      481 cgatcggcgt gccgttgcag atcgccaatc tttggacgac ctacaatctt cgacagctac
      541 ggcttccgga cgtcacgaca ggttttggtg tcgaatatcg gggcaagatt ttcggcgacc
      601 agctcaacac ggatgaggtc ccctcctatg tgatattcga cgcaaacatc acctattcgc
      661 agccgaaatg ggaccttggt gccggtatca agaacatcgc ggatacggtt tatttcaccg
      721 ctgccaacgg ggccggggcg tttgtcggcg atccgcgcac gttctacgtc aagggcgatg
      781 tcaaatttta gtgttagcgc cgaaaagtga aagcactccg gggaatcctg acaaataaga
      841 cagtctctga ctcataattc aattttttga atgagcggac ctgtctaaat cttccggata
      901 ctgcctgctg cggagatggc caaatatttc tgcagcggta ttcatgccgg agttttcatg
      961 acgctcgcga ttgacgatag tcgccggagc atcgcgctat ggggcggctt tgccatgatg
     1021 tgcgtcggca tgttcatggc gttgctcgac actcaggtag ttgtgacgtc gcttccgacc
     1081 attcagcgcg cgctgggcat tccgcaagat cagatgagct ggatccagac cgcttatctg
     1141 atctcggaaa tcatctcaat cccgctgacc gggcttttga cgcgcgtttt cggcatccgg
     1201 tggctgttca tcgtatcggt ttcgtttttc acgctggctt ccattggctg cgcgaacagc
     1261 gcgggctttt cctcgctgat cctctggcgc atctgccagg gatttgccgg cggaacgctg
     1321 atccctgcag tcttctccgc ggtttttctg ctctttccgc tccaccggca gaccttggcg
     1381 acgatgatcg ccagcgttct cgccgtcctg gcgccgacga ccggcccgct tgtcggcggc
     1441 tgggttacac agacctatag ctggccatgg cttttcctca tcaatgtcat ccccggcgtt
     1501 ctggtcgcga ttgccggaat ttacctcctg ccccgcgcgt cactgcgact cgatcagctt
     1561 aaagacctcg atttgatctc actgcaattt ggtgcgggcg cacttgctga gcttgagatt
     1621 gccatcaagg aagcgccggg ccgcgggtgg atttcgccgt gggtcctcgg acttttcgcc
     1681 tt
//
LOCUS       DASWOG010000540         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_13385,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000540 DASWOG010000000
VERSION     DASWOG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13385"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1296)
                     /locus_tag="VGB93_11100"
     CDS             complement(25..1296)
                     /locus_tag="VGB93_11100"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY0146672.1"
                     /translation="MTQDIDWKEAWRDHIWLPYAQMKDMEPPLAVSATRGSRIELADG
                     RELVDGIASWWTACHGYNHPHIRAAVMRQLESMPHVMFGGLIHEPAVRLASRLSALLP
                     GDLDRVFFSDSGSVAVEVALKMAAQYWLNKGVRGRTKMLAFRGAYHGDTFATMAICDP
                     DEGMHKHFAGILPEQIIADLPRDEDAMAALDALIAQHADKLAGIIVEPLVQGAGGMIF
                     HDAIVLSRLRALADKYDLLLIFDEIFTGFGRTGTMFACESADVVPDIICLSKALTGGT
                     MPLAATIARKKIFDAFWSDDPTHALMHGPTFMANALACAAANASLDLFAREPRLAQVA
                     KIESLLKAGLEPCRDMRGVKDARVRGAIGVVEFSKIDNIATLKRRFVAHGVFIRPFGD
                     IIYLTPALTISSDDLRRLTDAIVEVVRDELN"
     gene            1362..2501
                     /locus_tag="VGB93_11105"
     CDS             1362..2501
                     /locus_tag="VGB93_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0146673.1"
                     /translation="MKSLDAFARRKLAELDESSLRRGLVKTERAGGAIVMRGGRPLVS
                     FSCNDYLGLSQHADVKAAAIAATEAFGAGAGASRLVTGNYPLLYALEARLAQLKHTEA
                     ASVFGSGYLTNLGIIPALAGDGDLILIDELAHACIFAGGRLSPATTRVFLHNDVEAAN
                     AILKAERGRHRHALLITDHVFSMDGDRAPVTALADVCATHDTWLMTDDAHGLAVLGTQ
                     THQAHVPLQMGTLSKALGSYGGYLCASRDVIDLMTSRARPLVYSTALPPASAAAAIAA
                     LDLIAADPTLCARPLKKARDFAQRLGLPDAQSPIVPLVLGRAETALKASKILEDEGFL
                     VVAIRPPTVPEGSARLRFAFSAAHRDEDVARLADIVARLDLRAPA"
     gene            2515..3201
                     /gene="bioD"
                     /locus_tag="VGB93_11110"
     CDS             2515..3201
                     /gene="bioD"
                     /locus_tag="VGB93_11110"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY0146674.1"
                     /translation="MPALFITATGTDVGKTFVTTGLIRALRQHGRKVTALKPIGTGID
                     AENFAASDAALLLEACGRGVDLDAIAAISPWRFTAPLSPDMAAAEEGRTIDVSAVRHF
                     CTNAMAEAEDVSLIEGIGGLMVPLDARSTVCDLIVALKIPTILVAGTYLGSLSHTLTA
                     LEVAQKRDLHIAVLVLNETKNSTVKIEATRNSLSHFWKGPIVTIPHNALDASAFVQLM
                     EFLPPASSVA"
     gene            3236..4555
                     /locus_tag="VGB93_11115"
     CDS             3236..4555
                     /locus_tag="VGB93_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY0146675.1"
                     /translation="MPDPSRLTHLRIKTRDALRPWGRRTLFVIGGLLVGAAAILMAML
                     ADRAQHEFHQILGISRYLSLVLTPFGFGLIVFLTLHVFPNSQGSGIPQVIAARELPDG
                     PTRTALVSLRVAVGKIIAVTLGLFFGASIGREGPTVQVGAALMYAMGRRAMEYQRGLL
                     LAGAAAGIAAAFNTPLAGIVFGIEELSRSFESRTSGLVLGAIIAAGLTSLGLVGDYTY
                     FGSTAAVLPLGRAWLVVPICAVLGGLSGGLFSRIVVTFARGLPSQAGRMIAKHPIVFA
                     MLCGLGVAFTGLLSADTSYGTGYEQARAIVHGAVPSCAIFGLMKFFASLFSSIGGIPG
                     GLFAPSLAVGAGLAANLHPLFQDVPLGALALVGMVSYLTGVVQTPITSFVIVSEMTQN
                     HRMVIPLMLAALIADAVSKSICKDSIYHALAQVFLDRAAKTDAAPPE"
     gene            4552..5463
                     /locus_tag="VGB93_11120"
     CDS             4552..5463
                     /locus_tag="VGB93_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0146676.1"
                     /translation="MSSTDPEIPFDLRSPGAPDKLVRLSPLVRRIIADNPGPMTFTGT
                     CTYVVGAGDVAVIDPGPDSPTHLAALIEALGSERVCHIIVTHTHRDHSAGVVALKAAT
                     GADIIGCAPYIASEDGSSSGLEAGHDRTYRPDRVLADGDEIGGRGYVLKAVATPGHTG
                     NHLAFALPEERALFSGDHVMAWATTVVIPPDGKMGDYMASLEKLLARDDVQYWPGHGG
                     GLIEPQRMVRALFHHRRMREAAILAALDRGAADIPTLVASLYQGLDPRLHNAAGLSVL
                     AHLRDLGLRGLVQVPDGDGLAGVYSRT"
     gene            complement(5464..>5825)
                     /locus_tag="VGB93_11125"
     CDS             complement(5464..>5825)
                     /locus_tag="VGB93_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY0146677.1"
                     /translation="SDAAREAQTGAYPNVQPIYLDLEADDAYQAVLAAASALRWKIIN
                     AIPPGGRLGLGHVDAIAHGMILGFADDITIRIWPLAGETRVDIRSSSRIGRHDFGGNA
                     SRIEAFADELKTQANAK"
BASE COUNT         1076 a         1887 c         1706 g         1156 t
ORIGIN      
        1 aggcctgcca aaagccaagc ttcttcaatt gagttcatca cggacgactt cgacgatggc
       61 gtctgtcagc cgccgcaaat cgtcactgct gatggttaag gccggggtga gataaataat
      121 atcgccaaac ggccggatga aaacgccatg tgctacgaag cgtcgcttta gcgttgcgat
      181 attgtcaatt ttcgaaaatt cgacaacgcc gatcgcgccg cggacgcgcg catccttaac
      241 gccgcgcatg tcgcggcaag gctcaagccc cgccttcaac aatgattcaa tcttggcaac
      301 ttgcgccagc cgcggctcac gcgcgaaaag gtcgagcgac gcgttcgccg ccgcgcaggc
      361 caaagcattc gccatgaagg tcggcccatg catcagcgca tgggtcggat cgtcggacca
      421 aaaagcatcg aagatttttt tccgtgcgat tgtcgcggcc agcggcatgg tgcccccagt
      481 cagggctttc gacaggcaga tgatgtccgg cacgacatcc gcagattcgc aggcgaacat
      541 cgtgccggtg cggccaaagc ccgtgaaaat ctcatcgaag atcaaaagaa gatcgtattt
      601 gtccgcgagt gcgcgcaaac gcgacagcac aatcgcatca tgaaaaatca tcccaccggc
      661 gccctgcacc agcggctcga cgataatgcc cgcgagctta tccgcatgtt gtgcgatcag
      721 cgcgtcgaga gccgccatcg catcttcgtc gcgtggtaga tcggcaatga tctgttccgg
      781 caaaattccc gcgaagtgct tatgcatccc ctcgtcgggg tcgcagatcg ccatcgtcgc
      841 gaatgtgtca ccgtgatagg cgccgcgaaa ggcgagcatc ttggttctgc cgcggacgcc
      901 cttgttgagc cagtattgcg ccgccatttt cagcgcgacc tcgaccgcga ccgagccgct
      961 gtcggagaag aatacgcgat cgagatcgcc cggcaacagc gccgaaagcc gcgacgcgag
     1021 ccgcaccgcg ggttcgtgga tcaggccgcc aaacatgaca tgcggcatgg attcaagctg
     1081 ccgcatgaca gcggcgcgga tatgcggatg attatagccg tggcaggccg tccaccagct
     1141 tgcgatcccg tcgacgagct cgcggccatc cgcgagctcg atgcgcgaac cacgtgtcgc
     1201 ggaaaccgca agcggcggct ccatatcctt catctgcgca taaggcagcc agatgtggtc
     1261 gcgccaagcc tctttccaat caatatcttg cgtcaaaaga tacgccttgt tgccagagcc
     1321 gggcgccacg gttaaaccgc aacggcaacg aaggaaagcc cgtgaaatcc ctcgacgcat
     1381 ttgcgcgacg caaactggca gaactcgacg agtcgagcct gcggcgaggc ctggtgaaaa
     1441 ccgagcgcgc gggcggcgcc attgtcatgc gcggcgggcg cccgctcgta tccttttcct
     1501 gcaacgatta tctcggcctg tcgcagcatg cggacgtcaa ggccgccgcg attgccgcga
     1561 ccgaggcgtt tggcgcgggc gccggcgcct cgcgcctcgt cactggcaat tatcctttgc
     1621 tttacgccct tgaggcgcgg ctcgcgcaat tgaagcatac cgaagccgcg agcgttttcg
     1681 gctcgggcta tctgaccaat ctcggaatca ttccggcgct tgccggagat ggcgatctga
     1741 tcctcatcga cgaactggca cacgcctgca tcttcgctgg cggccggctt tcgcccgcga
     1801 cgacgcgtgt cttccttcac aacgacgtgg aggccgcgaa tgccattctc aaagccgagc
     1861 gcggacgcca tcggcatgcc ctactcatca ccgatcacgt tttctcgatg gatggcgacc
     1921 gcgcgcctgt aacggcattg gcggatgtct gcgcaacgca tgatacctgg ctgatgaccg
     1981 acgatgctca tggcctcgcc gttctcggca cgcaaacgca ccaagcccac gttccgcttc
     2041 aaatgggcac actgtcgaag gcactcggca gctacggcgg ctatctctgc gcatcgcgcg
     2101 atgtgattga tctcatgaca tcacgagcgc gcccgctcgt ctattcgaca gcattgccgc
     2161 cagcaagcgc cgcggcggcc atcgccgcgc tcgatctcat tgccgcagac ccgacgcttt
     2221 gcgcgcgacc gctgaagaaa gcgcgcgatt tcgcgcaacg gctcgggctg cccgatgcgc
     2281 aaagcccgat cgtaccgctc gtcctcggcc gggcggaaac cgcgctcaag gcctcgaaaa
     2341 ttctcgaaga cgaaggcttt ctcgtcgtcg cgatccggcc gccgaccgtg ccggaaggct
     2401 ctgcccggct tcgctttgct ttttccgccg cgcatcgcga cgaggatgtg gcacgcctcg
     2461 ccgacattgt cgctcggctc gatcttcgcg caccggctta gcgggaacgt catcatgccc
     2521 gctctcttca tcaccgcgac cggaaccgac gtcggtaaaa ccttcgtcac gaccgggctt
     2581 attcgtgctt tgcgccagca tggccgcaag gtcactgcat tgaaaccgat aggaaccggc
     2641 atcgacgcgg aaaacttcgc cgccagcgat gcggctctct tgctcgaagc ctgcgggcgc
     2701 ggcgtcgatc tcgacgccat cgcggcgatt tcgccctggc gcttcaccgc gccgctctcg
     2761 ccggatatgg cagcggcaga ggaaggcagg acgatcgacg tttcggctgt acggcatttc
     2821 tgcaccaacg cgatggcaga ggccgaggac gtatcgctca tcgagggcat cggcggcctc
     2881 atggtgccgc ttgatgcgcg atcgacggtc tgcgacctca tcgtagcgct caaaattcca
     2941 acgatcctcg tcgccggcac ctacctcggc agcttaagcc atacactcac ggcgctcgaa
     3001 gttgcacaaa agcgtgatct gcacatcgcc gtgctcgtcc tcaatgaaac gaaaaattcc
     3061 accgtaaaga tcgaggcaac ccgcaacagc ctcagccatt tctggaaggg gcccatcgtg
     3121 acgatcccgc acaatgctct cgacgcctct gcgttcgtgc aacttatgga atttttgccg
     3181 ccggcatctt ctgtggcatg aacgtctgac agattacact catacagcgc cgccgatgcc
     3241 cgacccaagc cgcctgaccc atctccgaat caaaacgcgc gacgcgttgc ggccttgggg
     3301 ccgccggacg ttgtttgtta tcggcggtct tctcgtcggc gcggcggcga tcctcatggc
     3361 gatgcttgcc gatcgcgcgc agcacgaatt ccatcagatt ctcggtatat cgcggtattt
     3421 gtcgctcgtg ctgacgccgt tcggcttcgg tctgatcgtc tttctcaccc tacatgtttt
     3481 tccgaattcg caaggcagcg gtattccgca agtgatcgcc gcgcgcgaat tgccggacgg
     3541 cccgacacgc accgcgctcg tatcgttgcg tgtcgccgtc ggaaaaatca tcgccgtcac
     3601 gctcgggctt ttcttcggcg cttcgatcgg ccgcgaaggg ccaaccgttc aagtcggcgc
     3661 cgcactcatg tatgcgatgg ggcggcgcgc catggaatat cagcgcggac tgctgctcgc
     3721 tggcgcagcg gcaggcattg ccgcggcttt caacacgccg ctcgccggta ttgtcttcgg
     3781 catcgaagaa ctgagccgct cgtttgaatc gcggacaagc ggcctcgttc tcggtgcgat
     3841 tatcgccgca ggcctgacct cgcttggtct tgtcggcgac tacacctatt tcggctcgac
     3901 cgctgcggtg ctgccgcttg gccgtgcctg gctcgttgtc cctatctgcg cggttctcgg
     3961 cggcctgtcg ggggggctgt ttagccgcat cgtcgttacc ttcgcgcgtg gcctgccgag
     4021 tcaggctggt cggatgatcg ccaaacaccc gattgtgttc gcgatgttat gcggactcgg
     4081 cgttgccttt acgggcctcc tttcagcaga tacgagctac ggcacgggtt acgagcaagc
     4141 gcgtgcgatc gttcatggcg ccgtgccatc ctgcgccatc ttcggcctca tgaaattctt
     4201 cgcgagcctg ttttcctcga taggcggcat tcccggcggc ctctttgcgc catcgctggc
     4261 agtgggcgcc ggcctcgccg ccaatcttca tccgctgttt caggatgtgc ctctcggcgc
     4321 gctggcactg gtcggcatgg tttcctatct cacgggtgtc gtgcagacgc cgattacgtc
     4381 gttcgttatc gtgtcagaga tgacgcaaaa tcacaggatg gtcatcccat tgatgctggc
     4441 cgctctcatt gccgatgccg tctcgaagtc aatctgcaag gacagcatct atcacgctct
     4501 ggcacaggtc tttctggaca gagctgccaa gaccgacgca gcgccgcccg aatgagcagc
     4561 accgatccgg aaattccctt tgatctccgc tcaccgggcg cacccgataa actcgtccgg
     4621 ctttcgccgc tggtgcggcg catcattgcc gacaatcccg gcccaatgac cttcaccggc
     4681 acgtgtacct atgtcgttgg cgcgggcgat gtggcggtca tcgaccccgg gcctgactcg
     4741 ccgacgcatc ttgccgcgct gatcgaggcc ctggggagcg agcgagtctg ccacattatc
     4801 gtcacccata cgcatcgcga ccattcggcg ggcgtcgtgg ccttgaaggc ggcaaccggt
     4861 gcggacatca tcggctgcgc gccctacatc gcatccgagg acggaagcag ttccggtctc
     4921 gaagccggcc atgatcgcac gtatcggccg gacagggtgt tggccgacgg cgacgaaatc
     4981 ggcggccgcg gctatgttct caaggccgta gcgacccccg gccatacggg caaccatctg
     5041 gcgtttgcgc tgcccgagga acgcgcgctg ttctccggcg atcatgtcat ggcctgggca
     5101 acgacggtgg tcattccgcc ggacggcaaa atgggcgact acatggcctc gctcgaaaaa
     5161 ctgctggcgc gggacgacgt gcaatattgg cccggccacg gcggcggcct gatcgaacca
     5221 caacgcatgg tccgtgccct cttccatcat cgcaggatgc gcgaggccgc gattctcgcg
     5281 gcactcgata gaggagctgc cgacattccg acgctcgtag cgtcactcta ccaagggctt
     5341 gacccgcgcc ttcacaatgc cgccggtctt tcggttcttg cgcacctgcg ggatctcgga
     5401 ttacgtggcc tcgtgcaggt ccctgatggc gacgggcttg cgggcgtcta cagccgcacc
     5461 tgattatttc gcgttggcct gggtcttcaa ttcgtcggca aaagcctcga tccgcgaggc
     5521 attgccgccg aaatcatgcc gcccaatccg tgatgaagaa cggatatcga cgcgcgtctc
     5581 cccggccaag ggccagatac ggatcgtgat gtcatcggcg aagccgagaa tcatgccatg
     5641 cgcaatcgca tccacatgtc cgagcccaag ccttccgccg ggcggaattg cattgatgat
     5701 tttccagcgt agggccgagg cggcggcgag cacggcctga taggcatcgt cggcctccag
     5761 atcgagatag ataggctgca cattcggata ggctccagtc tgtgcctctc gtgcagcgtc
     5821 cgaaa
//
LOCUS       DASWOG010000541         5495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_141779,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000541 DASWOG010000000
VERSION     DASWOG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141779"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGB93_11130"
     CDS             complement(<1..402)
                     /locus_tag="VGB93_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY0146678.1"
                     /translation="MHAFADPVSPAGWTMNLPVAVHLPGGLYFADTCYYMERQTAGAG
                     KVQGEVNLPALIWSTPFDFLGGHVEAIATVPELSIGDHTAGISSTSFYNSALLIGDVW
                     NLGSGWSVSEYVGTFFTVKTYNGETLGFGGDF"
     gene            657..1409
                     /locus_tag="VGB93_11135"
     CDS             657..1409
                     /locus_tag="VGB93_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0146679.1"
                     /translation="MTSSRQTSVRIQKDSSKALFAEKVRVCDYCELRNRGFCSRLTDA
                     QKAILADTARLVTVEPNKALFRQGDETENIYIVRSGLLRLYQRHLNGRRQVIGFAKPG
                     DLLLSPLNERNEYSADALDPVCLCHFRRQDFIAFFQRDPAFSAALAIAIHEELMRARQ
                     QIALLGQKRAEPKVAQFLLTLLESYAWRNVEATLVPVRVPQADIADFLGMTVETVNRT
                     LARFARDAIISVAKRGIDFLDLRRLKQIAESY"
     gene            complement(1406..2491)
                     /locus_tag="VGB93_11140"
     CDS             complement(1406..2491)
                     /locus_tag="VGB93_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEY0146680.1"
                     /translation="MIARVALATETRLKPQAFRLDKGQGVAEPAHEAYTLEPVEDFVE
                     AEAAAAAALDPAERAVESAQRRGITRGALISWGGLFFSAASGFVLVALSNWVFELIAD
                     LFSRSKALGYAAVGLGGLALLALAVMAGREIWSIGRQSKIAHMHMNFAAARTADDRDP
                     ARRLVTELVSLQSKRGSSTAVRANLLDLTKEIVDDPELIDIAERDLILPLDREVSREI
                     ATAAKRVSMVTAISPRALVDVIFVAAQALRLVRRISEIYGGRPGFFGFLKLIRSVGAH
                     LAITGGVAIGDSLIQQVVGHGIAAKLSARLGEGVLNGMLTARVGLSAMAVCRPMPFAI
                     ESPPGIGDVAPFLFQSDKKAAEDTRKS"
     gene            complement(2531..4030)
                     /locus_tag="VGB93_11145"
     CDS             complement(2531..4030)
                     /locus_tag="VGB93_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEY0146681.1"
                     /translation="MPSFADFLFDTGLAIQNLAESLSGRPLRLGVTGLSGAGKTIFLT
                     ALVQHLTLAARVATLGGKNPLPVFRVHAEGRLVRGAIQPQPDDAVPRFAYEDHIVTLT
                     GPGGDADKRSWPESTRRISELRLTLEFERDSGFGRRTSQLTIDLIDYPGEWLLDLPLL
                     GKSYRRWSHDTLDASLTPTRAPLAAEWRAYVTSLDPEAAADESVAQKAAALFTSYLRA
                     CRAEPISASALPPGRFLMPGDLEGSPLLTFSPLPVSEGSETDPQSLAGMMARRYDAYC
                     NHVVRPFFRDHFVRLDRQVVLVDVLAALNAGVSAVRDLEQALGDVLNAFRTGKNTLLT
                     SLLRPRIDKILFAATKADHLNHVSHDRLEAILRRLTARAIARSENVGAIIDVVALAAV
                     RATHEVNVGRGGAALPAVVGIPIAGERIGGEVFDGRTEAAIFPGELPRDPDAVFSGDM
                     GVIDPHAADYRFVRFRPPIAARDAAGAALPLPHIRLDRALQFLLGDRFD"
     gene            complement(4057..4572)
                     /locus_tag="VGB93_11150"
     CDS             complement(4057..4572)
                     /locus_tag="VGB93_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0146682.1"
                     /translation="MLRLAILRHAEAVPLTDESDADRELTPAGWEMAERMGRYFRDAG
                     LRPDLALVSPAKRAKETFRGLERGAGVTFTVDFVGKLYSADLRTLEEVVAEAPGDAKF
                     LLIVGHNPGLAELAHVLAGHGSKAELARMRGNFPAPALAVIDFDVDVWQKARRGVGQL
                     EHFVTRHALAK"
     gene            complement(4670..>5495)
                     /locus_tag="VGB93_11155"
     CDS             complement(4670..>5495)
                     /locus_tag="VGB93_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0146683.1"
                     /translation="FNGAIQEQPVVSFDLTDRGLNLGDGVFDTSLARNGRIFRRKSHI
                     DRMADASEILGIPFDEMAAGTALDALAEAIGDGAVRLTLTRGPGPRGLSFPKNPAPLL
                     YGAAAAMHTPLFPVLKLGTTSIRRNETSPASRIKALPYLDSVLALEQASARGADEVLF
                     ENTHSRVACLATANIFAIFGRQLVTPPLSDGALAGTTRAFVLSKAHGLHFDVAERSLP
                     LEKFSRADALFATSSLRLIAPCSQLDAITYPSEANEIVMELQTSIKEVIAAECGRF"
BASE COUNT         1140 a         1712 c         1702 g          941 t
ORIGIN      
        1 gaaatcgccg ccgaacccga gcgtctcgcc gttataggtt ttgaccgtaa agaaggtgcc
       61 cacgtactcc gacacgctcc agccgctgcc gaggttccag acgtcgccga tcagcaacgc
      121 cgaattgtag aaggaggtcg agctgatgcc ggcagtgtga tcgccgatgc tcaactccgg
      181 caccgtggcg atcgcctcga catggccgcc gaggaaatcg aacggcgtcg accagatgag
      241 agcgggcagg ttgacctcgc cctgcacctt gcctgcaccc gcggtctggc gctccatgta
      301 atagcaggtg tcggcgaaat agagaccgcc cggcagatgc accgcgaccg gcaggttcat
      361 cgtccagcca gcaggactga ccggatcggc gaaggcatgc acgccgcaaa tatagaatag
      421 tgccgcagcc gatgccgcgg atgctctcgc gagctttgtc gttgatcgtt tcatgcaagt
      481 ccccctccgg cttgtgtgcc gttgtcgggg aagatcgcat tcgtcgcgcg cgcggcaggt
      541 gatggggatc aagtcaacgt cacgtcggat cgcgacaaat tgtctccgcg tagtgtgatc
      601 tagatcaatt ctgctcgccg acgctggcgc gatgcttcgc gtgggaagga agcaaaatga
      661 catcgagtcg ccagacaagc gtgcggatac aaaaggattc gtcaaaggca ttgttcgcag
      721 aaaaagtccg cgtctgtgac tattgcgaac tacgcaacag aggcttttgc agccgcctca
      781 ccgatgctca aaaggccatc ctcgcggaca ccgcgcggct cgtgaccgta gaaccaaaca
      841 aggcactttt ccgtcagggc gacgaaaccg agaatatcta catcgtgcgc agcggcctgt
      901 tgcgactcta tcagcgccat ctcaacggac gccggcaagt gatcggcttt gcaaagcctg
      961 gcgatctgct gctctccccc ttgaacgagc gcaacgagta cagcgccgat gctctcgacc
     1021 ctgtttgtct ttgccatttt cgccgccaag atttcattgc ttttttccag cgcgatcccg
     1081 ccttttcggc tgcgttggcg atcgccatcc atgaagagtt gatgcgcgcc cgccagcaaa
     1141 tcgcgctcct cggccaaaaa cgcgccgagc caaaagtggc gcaattcctt ctcaccctgc
     1201 ttgaaagcta cgcgtggcgc aacgtcgagg cgacgcttgt gcctgtgcgg gtcccacagg
     1261 ccgacattgc cgattttctc gggatgacgg tggagacggt gaaccgcaca ctcgcgcgtt
     1321 ttgctcgcga cgcgatcatc agcgtcgcca agcgcggcat tgattttctt gacctgcggc
     1381 ggctcaaaca gatcgccgaa agctattagc ttttccgcgt atcttccgcg gctttcttgt
     1441 cggactgaaa gagaaacggc gcgacatcgc cgatgcccgg cggcgattcg atcgcgaacg
     1501 gcatcggccg gcaaacagcc atcgccgaca ggccgacgcg cgcggtcaac atgccgttga
     1561 gaacgccctc gcccagacgc gccgacaatt tggcggcaat gccgtggccg acgacctgct
     1621 ggatgagcga atcgccgatc gcgacgccgc ctgtgatggc gagatgcgcg ccgaccgagc
     1681 ggatgagttt gagaaatccg aagaagcccg gccgaccgcc atagatttcc gagattcgac
     1741 gaacaagacg caacgcctga gcggcgacaa agatcacatc gacaagcgcg cgcggcgaaa
     1801 tcgcggtcac catcgaaacg cgcttcgcgg cggtcgcaat ctcacgcgag acttcgcggt
     1861 cgagcggcag aatgagatcg cgctcggcaa tgtcgatcag ttctgggtca tcaacgatct
     1921 ccttggtgag atcgagaaga ttggcacgca cggcggtgct cgatccgcgc tttgattgca
     1981 ggctgacgag ttccgtcacg aggcgacgcg ccgggtcgcg atcgtccgcc gtgcgggccg
     2041 ccgcaaaatt catatgcata tgggcgatct tgctctggcg gccgatgctc cagatttcac
     2101 gcccggccat gacggcaagc gcaagaagcg ccaggccgcc gaggccgacc gccgcataac
     2161 ccaaagcttt cgagcgcgaa aatagatcgg cgataagttc gaagacccaa ttgctcaagg
     2221 cgacgagaac gaaaccgctc gccgccgaaa agaacagacc gccccaggaa atcagcgcgc
     2281 cgcgggtgat gccccgacgc tgcgccgatt cgacggctcg ctcggccggg tcgagcgccg
     2341 ctgcggccgc ggcttcggcc tcgacaaaat cctcgaccgg ttccagcgta tacgcctcgt
     2401 gcgcgggctc agcgacgccc tgccccttgt cgaggcggaa ggcctgaggt ttgagtcgtg
     2461 tttcggtcgc caaagccact ctcgcaatca tttcgatcgc atcggattgt ccgaactccg
     2521 atgcaacgcc ttaatcaaat ctgtcgccga gcaggaattg caaggcgcgg tcgagtctaa
     2581 tgtggggcaa cggcagcgcc gcacccgccg catcgcgcgc ggcgatcggc ggccggaaac
     2641 gcacgaaacg ataatccgcc gcgtgcggat cgatgacacc catgtcgccg gaaaagacag
     2701 catccggatc gcgcggcaat tcgccgggaa aaatcgccgc ttccgtgcgg ccatcgaaga
     2761 cctcgccccc gatgcgctca ccggcaatcg gaataccgac aacggccggc aaagccgcac
     2821 cgccacgtcc gacattgact tcatgcgtgg cacgcactgc cgcgagcgcg acaacgtcga
     2881 tgattgcgcc gacattctcc gaccgcgcaa tggcgcgcgc cgtcaaacgt cgcaggatcg
     2941 cctccaggcg atcgtggctc acatgattga gatgatcagc cttggtggcg gcaaaaagaa
     3001 ttttatcgat gcgcggccgc agcaaacttg tcagcagcgt attcttgcca gtgcggaaag
     3061 cattgaggac gtcgccgagc gcttgctcga gatcgcgcac ggcggagacg ccggcgttca
     3121 gcgccgccaa aacatcgacg agcacgactt gccggtcgag ccttacgaaa tgatcgcgaa
     3181 agaacggccg caccacatga ttgcaatagg catcgtagcg gcgtgccatc atgccggcga
     3241 gcgattgcgg atcggtttcg ctgccttcac tgaccggtag cggcgaaaac gtcagcagcg
     3301 gcgagccttc aagatcgccc ggcatcagga aacgacccgg cggcagcgcc gatgcggaga
     3361 tcggctccgc gcggcaggcg cgaagatagg acgtgaacag cgccgcagcc ttctgcgcga
     3421 cgctctcgtc tgccgcggcc tcaggatcga ggcttgtgac ataggcgcgc cattccgccg
     3481 ccagcggcgc acgcgttggg gtcaggctcg cgtcaagcgt gtcatgcgac cagcgtcgat
     3541 aggatttgcc gagcagcggc aaatcgagca gccattcgcc gggataatcg atgaggtcga
     3601 tcgtcaactg cgacgtccgc cggccgaacc cgctgtcacg ttcgaattcg agcgtcagcc
     3661 gcagttcgga aatgcggcgg gtcgactcgg gccatgagcg tttgtcggca tcgccgcccg
     3721 gccccgtcag tgtcacgata tggtcttcat aggcaaagcg cggcaccgca tcgtcgggct
     3781 gcggttggat ggcgccgcga accagacggc cttccgcatg tacccgaaag acgggcaacg
     3841 gatttttgcc gcccaatgtc gcgacacgcg cggccagcgt gagatgctga acgagcgcgg
     3901 tgaggaaaat cgtcttgccg gcgcccgaga ggccggtaac gcccagccgc aacggacggc
     3961 cggataggga ttcggcaagg ttctgaatcg cgagcccggt atcgaagagg aaatcggcga
     4021 aagagggcac gggcgatcct caaccttccg atgggttcat ttggcgagcg cgtggcgtgt
     4081 gacaaaatgt tcgagttggc ccacgccgcg acgcgccttt tgccagacat cgacgtcaaa
     4141 atcgatcacg gccagcgcgg gcgcgggaaa attgccacgc atccgcgcaa gttccgcctt
     4201 gctgccatgc ccggccaaga catgcgccag ttcggcgagg ccgggattgt ggccgacgat
     4261 gagaagaaat ttggcatctc cgggtgcttc agccaccacc tcctccagcg tgcggagatc
     4321 agcgctatag agtttgccga caaaatccac ggtgaacgtc actcccgcgc cgcgttcgag
     4381 gccgcggaaa gtttctttgg cgcgctttgc cggtgagacc aaagcgagat ccggccgcaa
     4441 tcccgcatcg cggaaataac ggcccatccg ctcggccatt tcccaccccg cgggggtcaa
     4501 ttcccgatcc gcatccgact cgtcggtgag cggcactgct tcggcatgac gcaaaattgc
     4561 taggcgcagc ataaggaaac cgggcaggag taaggacgaa ccgggaaccc ggcggcgcta
     4621 ttatctcccg cggcgggcac cttgcaaagc cgcgctcgga acgcccggtt tagaagcgtc
     4681 cgcattcggc cgcaatcacc tctttgatcg aggtttgcaa ttccatcacg atctcgttcg
     4741 cctcggaggg atacgtgatg gcatcgagct ggctgcacgg ggcgatcagc cgcaggctgc
     4801 tggtcgcgaa cagagcgtcg gcgcgggaga atttctccaa cggcagcgaa cgctcggcga
     4861 catcaaagtg caagccgtgc gccttggaaa gaacgaaggc gcgtgtggtc cccgcgagcg
     4921 cgccgtcgct cagcggtggg gtgacgagct gacggccgaa gatggcaaaa atgttcgccg
     4981 ttgcgaggca agccacgcgg ctatgcgtat tctcgaacaa aacctcgtcg gcaccgcgcg
     5041 cggaagcctg ctccagcgca agcacggaat cgagataggg cagcgccttg atgcgcgagg
     5101 cgggcgacgt ttcgttccgg cggatcgatg tcgtgccaag tttcaaaacc gggaacagcg
     5161 gcgtgtgcat cgctgcggcg gcgccgtaga gaagcggcgc tggatttttt gggaatgaca
     5221 acccgcgtgg cccggggccg cgcgtcagtg tcaaccgaac cgcgccatcg ccaatagcct
     5281 cggcgagcgc gtcgagcgcc gtcccggcgg ccatttcgtc gaacggaatc ccgagaatct
     5341 cggacgcgtc ggccatgcgg tcgatatgcg acttgcggcg gaaaatgcgg ccgttacgcg
     5401 ccagcgatgt atcgaaaacg ccgtcgccaa ggttgagtcc gcgatccgtc aaatcgaagg
     5461 aaacgaccgg ctgctcttgt atcgcaccat taaac
//
LOCUS       DASWOG010000542         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_49284,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000542 DASWOG010000000
VERSION     DASWOG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49284"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGB93_11160"
     CDS             complement(<1..215)
                     /locus_tag="VGB93_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146684.1"
                     /translation="MTIAFAAAVGHAPGMVAWPDAAPPAQKDALYAALDRLRRDLAAA
                     RVDEIILFTSEHWANFFLDHISAFCVGR"
     gene            complement(217..471)
                     /locus_tag="VGB93_11165"
     CDS             complement(217..471)
                     /locus_tag="VGB93_11165"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146685.1"
                     /translation="MSIYALQKTIREINRNHEMRQRFMAVPQDALADFDLTSEERDAL
                     IGRDYRALYRLGVHGLILRPFSIINGVSEADYLSAICEEP"
     gene            complement(471..794)
                     /locus_tag="VGB93_11170"
     CDS             complement(471..794)
                     /locus_tag="VGB93_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0146686.1"
                     /translation="MKKPTPVEYLRSINANSADAFQALRAAVTKAGPLDAHTVELIVL
                     GGLVTAGSERSFRTHALRLLKDKVDPQALRQAVLVTLAASASFNQVISALEWVDDVVR
                     EVEQA"
     gene            complement(801..>1538)
                     /locus_tag="VGB93_11175"
     CDS             complement(801..>1538)
                     /locus_tag="VGB93_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY0146687.1"
                     /translation="QGHLYSVESGELLAILDAQYLTTLRTGATSAVATRLMARPGQFH
                     VALLGSGVEARAQLEAMQAAGRVRSATVFSPTEANRNALTEDFRARHGMEIKAVASAQ
                     DAIEGAELVLAAVKSREPVLLGEWLKPGMHVNSVGTARRDQREIDVAVFERSARIAVD
                     TDAGVFGEAGDAVVAAPSLDRTKVHELHALASGQATGRMNDDEITLFKSVGTGIQDIA
                     LAAVIYANARAKGLGTDLGAFPYLKKN"
BASE COUNT          310 a          484 c          459 g          285 t
ORIGIN      
        1 cgcccgacac aaaatgccga aatgtgatca aggaaaaagt tggcccaatg ttcggaggtg
       61 aacaagatga tttcatcgac gcgcgcggcc gccagatcgc gccgcagccg gtctagcgcc
      121 gcataaagcg catctttctg agcaggcggc gccgcatcag gccaagcgac cattcccggc
      181 gcgtggccta cggccgcagc gaaagcgatc gtcatctcag ggctcctcgc agatggcgct
      241 tagataatcc gcttccgaga cgccgttgat gatcgagaac ggccgcagaa taagtccgtg
      301 aacgccaagg cggtaaagcg cacgataatc acggccgatg agcgcgtcgc gctcctcgct
      361 tgtgagatcg aagtccgcca gcgcgtcctg cggcacagcc ataaagcgct gccgcatctc
      421 gtgattgcgg ttgatctccc ggatcgtctt ctgcagcgcg taaatgctca tcaggcctgt
      481 tcgacctcgc gaacgacgtc atcaacccac tcaagtgccg agatgacctg attgaagctc
      541 gccgacgccg cgagcgtgac aagcacggcc tgacgcaggg cctgcggatc gaccttgtcc
      601 ttcagaaggc gtagcgcgtg cgtgcgaaaa ctgcgctcgc tgccagcggt cacgagtccg
      661 ccaaggacga tcagttcgac cgtgtgagcg tcgagcggtc ccgccttcgt aactgcagcg
      721 cgcaaggcct gaaaggcgtc agcggagttt gcgttgatgg aacgcagata ttcgaccggg
      781 gtcggctttt tcatgaggcc tcagttcttt ttcaagtaag gaaaggcgcc aagatcggtg
      841 ccaagtcctt tggcgcgcgc attggcatag atcaccgcgg caagcgcgat atcctggatg
      901 ccagtcccaa cggatttgaa cagcgtgatc tcgtcatcgt tcatccgccc agtcgcttgt
      961 cctgaggcaa gggcgtgcaa ttcatgcacc tttgtccggt caagcgatgg ggcagcgacg
     1021 actgcatcgc ccgcctcgcc gaagacgccc gcgtcggtat cgaccgcgat ccgcgcgcta
     1081 cgctcaaaca ctgcgacatc gatctcgcgt tgatcgcgtc gcgccgtgcc gaccgaattg
     1141 acatgcattc ccggcttgag ccattcgccg agcaaaacgg gttcgcgcga tttgacggcg
     1201 gcgagaacaa gctcggcacc ttcgatcgcg tcttgcgcgc tggcgacggc tttgatctcc
     1261 atgccgtggc gtgcacgaaa atcttcggtg agcgcattgc gattggcctc ggtcgggctg
     1321 aagactgtcg ccgagcgcac cctgcccgcc gcctgcatcg cctcaagttg cgcccgcgcc
     1381 tcgacgccgg acccaagcaa ggcaacatga aactggccgg gtcgcgccat cagccgcgtc
     1441 gcgactgcac tcgtcgcgcc ggtgcgcaat gtcgtgagat attgcgcatc caagatggcg
     1501 agcaattcgc cgctttccac agaataaaga tggccctg
//
LOCUS       DASWOG010000543         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_13450,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000543 DASWOG010000000
VERSION     DASWOG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13450"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..875)
                     /locus_tag="VGB93_11180"
                     /pseudo
     CDS             complement(116..875)
                     /locus_tag="VGB93_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
     gene            complement(899..1504)
                     /locus_tag="VGB93_11185"
     CDS             complement(899..1504)
                     /locus_tag="VGB93_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0146688.1"
                     /translation="MDEKIAAVLDAYHALIREERNKPWTTEDNGQDRRLRAVGPDTGQ
                     VINILAKSLKAPTILELGTSFGYSGIWLAEAARASGGRLITMELHDYKSAFARDMAIK
                     AGLVDYIDFRVGDAVQMIGDLQTSVDFVLVDLWKDLYVPCLDAFYPKLNPGAILVADN
                     MIYPGDEDVKHYGRAVRSKAGITSVLLPVGSGIEVSRFEPA"
     gene            complement(1557..2123)
                     /locus_tag="VGB93_11190"
     CDS             complement(1557..2123)
                     /locus_tag="VGB93_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0146689.1"
                     /translation="MRQRDKDGPDRHFASRDDPSDRHHHRHEGGLRRHGGRGRLFDYG
                     ELRLLLLAMIAERPRHGYEVIKAIEERFGGSYIPSPGVIYPTLAWLDDMGYAAIDAVE
                     SGRKLYRLTAEGEAFLVANRAAADELQSRLRPEGGGPASAPASIVRAVENLKLALRLR
                     LKRGTINQGAAESIAAALDAAAQTVERS"
BASE COUNT          511 a          773 c          741 g          503 t
ORIGIN      
        1 cgatgatcgt ctcgcctcgc tcggttatcc ctttgaggat gaaaccgcga atgatgccta
       61 ccgactgttc ttacgctagc tgagggaccc gtccccccag gattttgtcg gttccctatc
      121 tgcccggcga tcggtcggcc aacttgtcat gggcatcggc gacaccctta cgccaatagc
      181 cggctgcctt catccaaacg agcggatggc cacatgaatt gacaaggtaa tcgcgcgcgg
      241 cgcgggcgac ggatgcctcg gctgcgatcc agacaaaacc atctcccttc ggaagcgaaa
      301 gcgtctcgat cgccgaaagc agtgaggccg gatcgttggc gtgatcaagc ggccgatgaa
      361 cccagacagc ctcgtggtgc gcgcgggtgg caaaggtttg ttcctccacc tgagccgcaa
      421 ccgacacaac actgatgacg cgcgcgctct gtcatctcct caatgcggcg gccgatcgca
      481 ggcggcgccg tctcatcgcc gatcagaagc caccagtcga aggtcggcga caccacggtt
      541 gagccgcgcg gaccgccaat ctcgatctga tcgcccggtt cggcctcgat cgcccaacgc
      601 gtggccggac ctgcgtcatg gattgcgaaa tcgatggtca atgtgtgcgc cactggatcg
      661 taacgccgtg gcgtgtaatc gcgccgctcg ccttggcctg cttgcgacgg aatgaacagc
      721 ttcacatgat cgtccgggcc caaactcgcg aaatctgcga ggtcgtttcc cgcgaacaca
      781 atgcggatca tgccgggcgt gatccggctt ttctccttga cggtcagact tcgacgcttc
      841 ggctccagcc gatgccgggt gatttcatgc gccatcacga actccgaaaa ttctcctgtc
      901 aggccggctc gaagcggctg acttcgatcc ccgagcccac aggcagcagc acgctggtga
      961 tcccggcttt ggagcgaacg gcacggccgt aatgtttcac gtcctcgtcg cccggataga
     1021 tcatgttatc ggcgacgagg atcgcgcccg gattgagctt gggataaaaa gcgtcgagac
     1081 acgggacata gagatccttc cagagatcga cgagaacgaa gtccacgctg gtctgcagat
     1141 caccgatcat ctgcacagcg tcgccaaccc ggaaatcgat ataatcgacc agccccgcct
     1201 tgatcgccat gtcacgcgcg aaggcggact tgtaatcgtg cagttccatc gtgatcagcc
     1261 ggccaccgct tgcccgcgca gcctccgcca gccagatgcc ggaataaccg aaggaagtac
     1321 caagctcgag gatggtcggc gccttgaggc ttttagccag aatgttgatg acctgtccgg
     1381 tgtcaggccc gaccgcacgc aggcgacgat cctggccatt atcttccgtc gtccacggct
     1441 tattgcgttc ttcgcgaatc aacgcgtgat aggcgtcgag cactgcggcg atcttctcgt
     1501 ccatgcgtaa atcccgtcgt atgaggtgtc gtcgaattat tgaccgcagc cgtgagtcaa
     1561 ctgcgctcca cggtttgcgc cgcggcatcg agcgcggccg cgatactttc cgcggcaccc
     1621 tggttgatcg ttccgcgttt gaggcgaagg cgaagggcga gcttcaggtt ctccacggcc
     1681 cgcacgattg acgccggagc ggaagccggg ccgccgccct ccgggcgcag gcgcgattgc
     1741 aactcgtcgg cggccgcgcg attggcaacg aggaaggcct cgccttcggc ggtgaggcga
     1801 tagagcttgc ggcctgactc cacagcatcg atcgcggcgt aacccatgtc gtcgagccag
     1861 gcgagcgtgg gataaatcac gccggggctt gggatgtagc tgcccccgaa gcgctcctcg
     1921 attgctttga taacctcata cccgtgacgc gggcgctcgg cgatcatcgc caggaggaga
     1981 aggcgcagtt cgccatagtc gaagagtcgg ccacggccgc cgtgccggcg aaggccaccc
     2041 tcatgacggt ggtgatgacg atccgacgga tcgtcccggg atgcaaaatg acggtccgga
     2101 ccgtccttgt ctctttgtct catggtcgct gacctatatc gagatatagc tagattcaag
     2161 atatatctag ataatattcg tttaccgaat tgacattcag actggcaagc aagcgatcgg
     2221 atcaaccgat ctcaggcggc cgtgattccc ggtcagcggt ctcgattttt tccgcatcga
     2281 tttcttttgc ggcgcgggaa gaattgcctc gggggagcgc ggcaaagagg aaattgctct
     2341 ctttctcaaa aacgactagg cataaaggat taacgccggg tcccgctcgt ctcgatgctc
     2401 acaagcaaag caaaatacgg cctcaaggcg ctgatgcatc tcgcgaccta tgacggtccg
     2461 tgtctcgcgg aagagatcgc caaggagaac aatagtcctc gcaattttct cgaggccatt
     2521 atcgtcga
//
LOCUS       DASWOG010000544         4367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_141829,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000544 DASWOG010000000
VERSION     DASWOG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141829"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..693)
                     /locus_tag="VGB93_11195"
     CDS             complement(226..693)
                     /locus_tag="VGB93_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0146690.1"
                     /translation="MKKVLVLAAGSAALLLSCVSAFAIESEYKATSTHSVGDVWAKVG
                     DFCGIGTWHPAVAKCELSFGKKIRTLSLKGGGTIVERLVSWDKADHKYTYRIVKSPLP
                     VKDYISTIKVVADGSGSDVIWTGKYKAVKGTTDDAAKKVVDGIYKAGADSLAQ"
     gene            complement(862..1029)
                     /locus_tag="VGB93_11200"
     CDS             complement(862..1029)
                     /locus_tag="VGB93_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146691.1"
                     /translation="MLKKIVAAIVLASAVAFAPVAAMADHVVVVHHGGFHHDYHHHHH
                     VVVIHHHRDHY"
     gene            1221..1757
                     /locus_tag="VGB93_11205"
     CDS             1221..1757
                     /locus_tag="VGB93_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEY0146692.1"
                     /translation="MSEIWLRTGEATVLAAEGQFTDAMPEVMIGDVRGPVGQAFVSMA
                     GQVAGHPRMFVIRDLNQQVRPATMMTTKMTVRSEAYVELLGGIVQAATGDAIVDCLSE
                     GILPKDKANDLCMIIMIWLDPRCATDENLDRRDLYRTNYDATKLAISRAMKGEPTVEQ
                     LIANRKTISHYALEGVFD"
     gene            complement(1758..2066)
                     /locus_tag="VGB93_11210"
     CDS             complement(1758..2066)
                     /locus_tag="VGB93_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146693.1"
                     /translation="MSEALHAQRRARFFAFVVVLLDAMAYFFLVRTYWFTMHEPATRN
                     FDIVAGLVVSFFFCMSAIPALILLLLRAQSRTAILFALGFPVACVLAFAAGAAIFTWA
                     "
     gene            complement(2063..>4367)
                     /locus_tag="VGB93_11215"
     CDS             complement(2063..>4367)
                     /locus_tag="VGB93_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY0146694.1"
                     /translation="GTAQSAFSNFEAQRKALNKNLRDETQRYPQCEAELAKVSTELAA
                     LRTENENASAADLFSAAVEAARAAAEAAEKDMLAAEAAHAESRRAEAETRGPWQDAER
                     KAQSLATEAQTLAKLLSAPSGSRWPAVVEEISVAKGYEAALGAALGDDLDASIDVTAP
                     AYWDLLSGYDDPALPAGVDSLAQHVSAPSALARRLVQIGVVAQSTGKALQAQLRPGQR
                     LVTMEGDLWRWDGFCLAAEAPTPAARRLAEKNRLGDLTRDAEIARHSADEVKAKAETA
                     QADLRVKAEAEAKVRQAHRDALRAVENTREAAAEAERKKAQVAARLSGREEAAQRLTA
                     SRDEARANQAKAEAALAELVATPELAMALEAARVKAAQDRAATAEARAHLQAQMHEMQ
                     TRTKRLAAIGEERGSWNNRRERAQSQIGELDSRLKEAAIEQEKLADAPGEFLRLRRNL
                     MDQIETAENTRRAAAEARAKAEERLADADRAARKALDAMSTAREEKARDEARLEGARE
                     RFAAITHHIETELQCDPRGLATLAGVERDDELSDAPSVEAKLEKLKAERERLGNVNLR
                     ADEELTESETRRTNLTSEREDLTEAIRRLRQAIQNLNKEGRERLLGAFDAVNAHFKDL
                     FTTLFGGGAAELKLVESEDPLEAGLEILAQPPGKRMQALSLLSGGEQALTAMSLIFAV
                     FLTNPSPICVLDEVDAPLDDANVEHFCDLLEQMVKRTDTRFVAITHNPISMARMDRLF
                     GVTMAERGISQLVSVELTEVEHFLEAV"
BASE COUNT          810 a         1344 c         1334 g          879 t
ORIGIN      
        1 acgagtggtt atcccgaata cgatcacgag gccgtctcgg ccgttcgccg cgccgcgccg
       61 tttccgcagc cgccttatgg cgaaccgcac ggctttatcg ccaagatgga ttttccggcg
      121 cggtagattt tccggcgcgt aatggcggaa acgaaaacgg tctccgcagc cgcgctgcag
      181 agaccgcctg acagaaaatg acaattatgt caggaagcgc tggctttatt gcgccagcga
      241 atccgcgccg gctttgtaga tgccgtcgac gactttcttg gcggcgtcgt cagtcgtacc
      301 cttcaccgct ttgtatttgc cggtccagat gacgtccgaa cccgaaccat cggcgaccac
      361 cttgatcgtc gagatataat ccttcaccgg cagcgggctt ttgacgatgc gataggtgta
      421 tttgtgatcg gctttgtccc acgacacgag ccgctcgacg atggtgccgc cacccttcaa
      481 actcagagtg cggatcttct tgccaaacga cagttcgcat tttgcaacag caggatgcca
      541 ggtgccgatg ccgcagaaat cgccaacttt cgcccagacg tcaccgacgg aatgggtgga
      601 tgtggctttg tattccgatt caatcgcgaa ggccgatacg caggacaaca acaacgcggc
      661 cgagccggcc gcgagcacta atactttttt catagggcgt ttctcccatt attcccatga
      721 tctctgggct ctttagctta gcctcgggca ccacatgctg gcaatcccgc ggtctggtgc
      781 aacattattt ggttggcccg caaagaaacg cgccgggaaa ggtcccggcg cgaaacaatc
      841 atcagcaatc agacgcgtcg cttagtaatg gtcgcgatga tgatgaatca caacgacgtg
      901 gtgatggtgg tggtaatcat gatgaaagcc gccatgatgc acgacgacga catggtcggc
      961 catggccgcg acaggcgcga aggcgacggc cgaagccagg acgatcgccg caacaatttt
     1021 cttcaacatc caaatcccct tattgtgttt ctgttcgaga gccacggacg tgccgtgatt
     1081 ccctcctcga aatatctaaa tcgcgacctt aacggcggct gaacttggcc gtaagcgatg
     1141 cattcaaagc ggcgctgggt taggccttgg acaaacagct tgcgggtgtt atgcgagcgc
     1201 gaaaagcaag gggtaacgaa atgagcgaga tttggttgcg tacaggcgaa gcgacagttt
     1261 tggcggcgga ggggcaattc accgacgcaa tgccggaggt gatgatcggc gacgtacgcg
     1321 ggccggtggg gcaagcgttc gtgtcgatgg cggggcaggt cgcgggtcac ccgcgcatgt
     1381 tcgtcatccg cgaccttaac cagcaggtcc gcccggcgac catgatgacc accaaaatga
     1441 cggtgcgatc agaagcttat gtcgaattgc tgggcggcat cgtccaggcg gcgacaggcg
     1501 atgcgatcgt cgattgcctg tccgaaggga tactgccgaa ggacaaggcc aacgatctct
     1561 gcatgatcat catgatctgg ctcgatccgc gctgcgccac agacgaaaac ctcgatcgcc
     1621 gcgaccttta tcgtaccaat tacgacgcga cgaaactcgc gatctcgcgc gcgatgaagg
     1681 gcgagccgac ggtcgagcaa ttgatcgcca accgcaagac tatcagccat tacgcgctcg
     1741 aaggcgtgtt cgattgatca cgcccatgtg aaaatggcgg ctccagcagc gaacgccagc
     1801 acgcaggcga caggaaagcc aagcgcgaaa agaattgcag tccgtgactg cgcgcgcagc
     1861 agcaaaagga tgagtgctgg aatcgcgctc atgcaaaaga aaaacgaaac aacgagtccg
     1921 gcgacgatat cgaaatttct cgtcgctggt tcgtgcatcg tgaaccaata agttcggaca
     1981 agaaagaaat aagccatcgc gtcgagcaag acgacgacga aagcgaagaa acgcgcgcga
     2041 cgctgcgcgt gcaacgcttc gctcacaccg cctcgaggaa gtgttcgact tcggtcagct
     2101 ccaccgacac caattggctg atgccgcgtt cggccatcgt cacaccgaag agccggtcca
     2161 tccgcgccat cgagatcggg ttatgcgtga tggcgacgaa gcgcgtgtcg gtgcgcttca
     2221 ccatctgctc aagcagatcg cagaagtgtt cgacgttggc gtcgtcgagc ggcgcatcga
     2281 cctcatcgag cacgcagatc ggcgacggat tggtgaggaa gacagcaaag atcagcgaca
     2341 tcgccgtcaa agcctgctca ccgccggaca gaagcgagag cgcctgcatg cgcttgcccg
     2401 gcggctgcgc gagaatttcg aggccggctt cgagcgggtc ttcggattcg acaagcttca
     2461 attccgccgc gccgccacca aagagtgtcg tgaagagatc cttgaaatgc gcgttcacag
     2521 cgtcgaaggc gccgagcagc cgttcgcggc cttccttgtt gaggttctgg atcgcctgac
     2581 gcagacggcg gatcgcttcg gttaaatcct cgcgctcgct cgtcaggttc gtccggcgcg
     2641 tttcggattc ggtcaattcc tcatcggcac gaaggttgac gttgcccagc cgttcacgct
     2701 ccgccttcag cttttcgagt ttggcttcga cgcttggcgc atcgctcaat tcgtcatcgc
     2761 gctcgacccc ggcgagtgtt gcaagacctc tgggatcgca ttgcaattca gtttcgatgt
     2821 gatgggtgat cgcggcgaag cgctcacgtg cgccttcgag gcgtgcctca tcacgcgcct
     2881 tttcttcgcg tgccgtgctc atcgcatcga gcgccttgcg cgccgcgcgg tcggcgtcgg
     2941 ccaggcgttc ttcggctttg gcgcgtgcct ctgcggcagc acggcgtgtg ttctcggccg
     3001 tttcgatctg atccatcaga ttgcggcgca ggcgcaaaaa ttcgcccggc gcatcggcga
     3061 gtttttcctg ttcgatcgcg gcttccttga gacgtgagtc caattcgccg atttgagatt
     3121 gcgcacgctc gcgccgatta ttccacgagc cgcgctcctc gccgatcgcg gcaagacgct
     3181 tcgtccgcgt ctgcatctca tgcatctgcg cttgcagatg cgcgcgcgct tcggcggtcg
     3241 cggcgcgatc ctgcgccgcc ttgacccgcg ccgcctccag ggccatcgca agttcgggcg
     3301 tcgcaacgag ttccgccaac gctgcttccg ccttggcctg attggcgcgg gcttcgtcgc
     3361 ggctcgccgt caggcgctgc gccgcttcct cgcgccccga aagccgcgcc gcgacttgcg
     3421 ccttcttgcg ctccgcttcg gcggccgctt cgcgtgtgtt ttcgacagcg cgcaacgcgt
     3481 cgcggtgtgc ctgccgcacc ttggcttcgg cctcggcttt cacccgtagg tcggcctgcg
     3541 ccgtctcggc tttggctttg acctcgtcag cggaatgacg tgcgatctca gcgtcgcgcg
     3601 tcaggtcgcc aagccggttc ttttcggcga ggcgccgggc cgccggcgtc ggtgcttccg
     3661 ccgcaaggca gaagccgtcc cagcgccaga gatcgccttc catcgtgacg agtcgctgac
     3721 cgggccgcaa ttgtgcctgc aaggccttgc cagtgctctg ggcgacgacg ccaatttgca
     3781 ccaaacgtcg cgccagagcg gagggcgcgc tgacgtgctg cgcgagcgag tcgacgccag
     3841 cgggcagggc gggatcgtca tagcccgaga gcagatccca ataggccggg gcggtcacat
     3901 cgatcgaggc gtcgagatcg tcgccaaggg cggcgccgag cgccgcctca tagcctttgg
     3961 cgacgctgat ttcttcgaca accgcgggcc agcggcttcc gcttggcgcg gagagcagtt
     4021 tcgccaacgt ctgggcttcg gtcgcaagac tttgcgcctt gcgttccgcg tcctgccagg
     4081 ggccgcgcgt ttccgcttcg gcgcgccgcg actcggcatg ggcggcctcg gcggcgagca
     4141 tgtccttttc tgcggcctcg gcggcggcac gtgccgcctc gaccgcggcg ctgaacagat
     4201 ctgccgcgct cgcattttcg ttctcggtgc gcagggctgc tagttcagtg ctcaccttcg
     4261 caagctcggc ctcgcattgc ggatatcgct gcgtctcgtc gcggagattt ttattcagcg
     4321 ctttgcgctg cgcttcgaaa ttcgagaagg cgctttgcgc ggtgccg
//
LOCUS       DASWOG010000545         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_67906,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000545 DASWOG010000000
VERSION     DASWOG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67906"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1366
                     /locus_tag="VGB93_11220"
     CDS             215..1366
                     /locus_tag="VGB93_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0146695.1"
                     /translation="MSDSRRPPIFPLTILTGFLGAGKTTLLNQLLRAPELSDSLVLIN
                     EFGEVGLDHLFVEKVDGNVVVMASGCLCCTIRGDLVSTLEDLLRKRDNNRIAPFNRVI
                     IETTGLADPAPVLHTIMYHPYFMLRFRLDGVITLVDALNGAATLDRHPEAVKQAAVAD
                     RLVISKSDLLPADDVGLAALHSRLKALNPTAIQLDAARGEATPAALFNIGLYDPDKKT
                     PDVRRWLNAEALDDHAHAHHHHHDHSGHDHAHAHDVNRHDARIRAFALRHPTPVPPAM
                     VDMFLELLRTAHGAHLLRVKGIVALDDDPARPLVIHGVQHIFHPPARLDSWPDDDHTT
                     RIVFILHDMEPSFIQGMWNAFVGVAADTPDAAALTQNPLKVSSGGGLLA"
     gene            complement(1363..>2271)
                     /locus_tag="VGB93_11225"
     CDS             complement(1363..>2271)
                     /locus_tag="VGB93_11225"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0146696.1"
                     /translation="AATSAVERWLEVSFLPHTRLDLGLRNAIKTSIGYIGRIFALGFA
                     LAYLGVDFAKLAIVAGALSVGIGFGLQSIVNNFVSGLILLWERAIRVGDWISVGPNEG
                     FVRRINVRSTEIETFDRAAVVIPNSDLVAGVVKNFVRTDKTGRLKLNIPVNAAADPQK
                     AREVLLDVTKSNKNVLKKPAPFVVFAEITGGVFNFELYCYVSDVTSMKSITSELNFEI
                     YRRFKDASLFDAPPPVMNINLPELEKRGFVLREQVDRNPANDQEAPADDDKAFSGEAD
                     KGPREENGSKQKNQSPASDPIRSKKL"
BASE COUNT          456 a          680 c          656 g          479 t
ORIGIN      
        1 ggtaaaagta aagagcaaaa cggcgaaacc ggcgagcaaa aaggccaagg cgaagaacgc
       61 cgctggcaag agccacggga aacatttgtc gcacgcaagc cattgaggct cgcggcgcgg
      121 tccgattgaa tcggagcggt ccgcggctta tctctctcca gcacgatctt ttgcccggac
      181 gcacggtccg cgcgatcgcg ctggatttta agagatgtct gactcgcgcc ggccgccgat
      241 ctttccactg acgatcctca ccggctttct cggcgccggc aagacaacgc tactcaatca
      301 attgctgcgc gcgccggagc tctcggatag tctggtgctc atcaatgaat tcggcgaggt
      361 cgggctcgat catctctttg ttgagaaagt cgatggcaat gtcgtcgtca tggcatccgg
      421 gtgcctttgt tgcacgatcc gcggcgacct cgtctcgacg ctcgaagatt tgctgcgcaa
      481 gcgcgataac aatcgcatcg cgccattcaa tcgtgtgatc atcgagacga ccggcctcgc
      541 cgacccggcg ccggtactgc atacgatcat gtatcatccc tatttcatgc tgcgctttcg
      601 ccttgacggc gtcatcacgc ttgtcgatgc attgaacggc gcggcgacgc tcgaccggca
      661 tcctgaagcg gtcaaacagg cggcggtggc cgaccggctc gtcatcagca agagcgatct
      721 tctgccagca gacgatgttg gccttgcggc attgcatagc cgcctcaagg cgctcaatcc
      781 gaccgcgatt cagcttgatg ctgcgcgcgg tgaagcgacg ccggccgcgc tgttcaacat
      841 tggtctttat gatccggaca agaaaacacc cgatgtgcgc cgctggctga atgccgaggc
      901 gctggatgat cacgcgcatg ctcaccatca ccatcacgat cattccgggc acgaccatgc
      961 gcacgcgcac gacgtcaacc ggcacgacgc gcgaattcgc gcttttgcgt tacgtcaccc
     1021 gacgcccgtt ccgccagcca tggtcgatat gtttctcgaa ttgctgcgaa cggcacatgg
     1081 cgcgcatctc ctgcgcgtga agggcatcgt cgcgctcgat gacgatccgg cgcgcccgct
     1141 cgtcattcat ggcgtgcagc atattttcca tccgccagcg cggctcgatt catggcccga
     1201 cgacgaccat acaacgcgca tcgtcttcat cctgcacgat atggagccga gcttcattca
     1261 aggcatgtgg aatgccttcg ttggcgtcgc agccgatacg cccgatgccg cggcgctgac
     1321 acaaaatccg ctgaaagttt cgtctggcgg cggcttgctg gcctagagct ttttgcttct
     1381 gatgggatca gaagcagggc tctggttttt ttgttttgat ccgttttctt cacgcggacc
     1441 tttatccgct tcgcccgaaa acgctttgtc gtcgtccgct ggcgcttcct ggtcgttggc
     1501 gggattgcga tccacctgtt cgcgcaggac gaagccgcgc ttttcgagtt ccggcagatt
     1561 gatgttcatc accggcggcg gcgcgtcgaa caaacttgca tccttgaagc ggcgatagat
     1621 ttcaaagttc aattcgctcg tgatcgattt catcgacgtg acatcgctca cgtagcaata
     1681 aagctcgaag ttgaagacac cgccggtgat ctcggcgaag acgacaaagg gcgccggctt
     1741 tttcagaacg ttcttgttgc tcttggtcac gtcgagaaga acttcgcgcg ctttttgcgg
     1801 gtccgcggcg gcgttcaccg gaatgttgag cttgagccgg cccgtcttgt cagtgcggac
     1861 aaagtttttg acgacgccgg cgacgagatc cgaattggga atgacgaccg ccgcgcgatc
     1921 gaacgtctct atctcggtcg aacgcacatt gatgcggcgt acgaagcctt cgttggggcc
     1981 aacagaaatc cagtcgccaa cacggattgc ccgttcccaa agcaggatca ggccagagac
     2041 gaaattgttg acgatcgatt gcaggccaaa gccgataccg acggacagcg cgccggcgac
     2101 gatggcaagt ttggcgaaat cgacgccgag ataggcgagt gcaaagccca gcgcgaaaat
     2161 gcgcccaata tagccgatgc tcgtcttgat ggcgttgcgc agcccgagat cgagccgcgt
     2221 atgcggcaga aagctcactt cgagccagcg ctcgactgcg ctcgtcgcgg c
//
LOCUS       DASWOG010000546         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_13467,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000546 DASWOG010000000
VERSION     DASWOG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13467"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VGB93_11230"
     CDS             <1..408
                     /locus_tag="VGB93_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEY0146697.1"
                     /translation="GDTFRWKGENVSTFEIAEALNAVPGVVDATAYGVVVPGADGRAG
                     MATLVIAPDFDLKLLHARLGALLPSYARPLFLRISAEIEATETFKQKKAQLAREGFDP
                     GKIEDQLYLDDGIGYVPLDADLFAKISAGEVRL"
     gene            complement(383..1312)
                     /locus_tag="VGB93_11235"
     CDS             complement(383..1312)
                     /locus_tag="VGB93_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0146698.1"
                     /translation="MTDAFDPYAKRFLDMLALKGGSVASLDIEARRTAFANLLRFCAP
                     GSPIASVEEIDAAGRTLRLYRPEARSATAMVFLHGGGLVAGSLETHDALCRTLAAANG
                     CNIFAVDYRLAPEHPFPAAFDDTLSALEWIFEKAPAYGVKRIGIGGDSAGATLATAAA
                     AEWGMQEKPGLAFQLLLCPILDFAGETPSRHAFASPVLDQATLDHDIALYTGGTVGAD
                     DPRISPLRAADFSGLPPTFLHIAQCDPLRDEGADYANKLRAAAVEVHHTCHAGMPHLF
                     YALSGVIPAAKTVVAAIGAEVSTWLATSGPLRH"
     gene            1399..2148
                     /locus_tag="VGB93_11240"
                     /pseudo
     CDS             1399..2148
                     /locus_tag="VGB93_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
BASE COUNT          449 a          656 c          658 g          425 t
ORIGIN      
        1 ggtgacacgt tccgctggaa aggcgagaac gtctctacgt tcgaaattgc cgaagcgctc
       61 aacgccgtcc ccggcgtggt cgacgcgacc gcctatggcg tggtggttcc cggcgccgat
      121 ggacgcgccg gcatggcgac actcgtcatt gcgccggatt tcgatttgaa gctcctgcac
      181 gcgcggcttg gtgcgctgtt gccttcctat gcgcgtccgc tctttctgcg gatcagcgca
      241 gagatcgagg ccacggaaac cttcaaacag aagaaagctc aactcgcgcg cgagggcttc
      301 gaccccggca agattgagga tcaactctat ttggacgacg ggataggcta tgtgccgctc
      361 gacgccgatc tcttcgcaaa aatcagtgcc ggagaggtcc gcttgtagca agccacgtcg
      421 atacctcggc gccgattgcg gcaacgacgg tcttcgccgc ggggatgacg cccgataatg
      481 cgtagaaaag atgcggcata ccggcatggc acgtatgatg gacttcgact gccgccgcgc
      541 gaagtttgtt ggcatagtct gcgccttcgt cgcgcaatgg gtcgcattgt gcaatatgca
      601 gaaatgtcgg cggcaggccg gaaaaatccg cagcacgcaa tggcgagatt cgcggatcgt
      661 ccgctcccac cgtgcccccg gtgtaaagcg cgatatcgtg gtcgagcgtc gcctgatcga
      721 ggaccggtga tgcgaacgca tggcgcgacg gggtttcacc ggcgaaatcg agaattggac
      781 aaagcaaaag ctggaaagcc aagcctggtt tttcctgcat cccccattcg gccgcggcgg
      841 cggtggccag tgtcgcgccc gctgaatcgc caccgatccc aattcgcttg acgccataag
      901 ctggcgcctt ttcaaaaatc cattcgagcg ctgacagagt atcgtcaaat gccgcgggaa
      961 aaggatgttc cggcgccaac cgataatcga cggcgaaaat gttgcagccg tttgccgccg
     1021 ccaatgtgcg acagagggca tcgtgcgtct cgaggctgcc ggcgacgagg cctccgccgt
     1081 ggagaaagac catcgccgtc gcggatcgag cctccggccg gtaaagccgg agcgtgcgcc
     1141 cggcagcgtc gatctcttcg acactcgcga ttggcgaacc cggcgcgcaa aatcgcagga
     1201 gattggcaaa agccgtgcga cgcgcctcga tatccaacga tgcgacgctg ccgcccttca
     1261 gcgcgagcat atcaagaaaa cgtttagcat agggatcgaa agcgtcggtc acagaagatt
     1321 atactcgtga gggcaatttt tcgcctgcga gcctataaga attgccttaa gactgcgcta
     1381 cgaaaattct catctcccat gcgaggactc gttttgtcta gcgtagtagg cgtcgcgccg
     1441 accaagacct ggaccttttt tgaaagcaac tggcacgagg gcaatgtcgc catcatgggg
     1501 ccgcgcaccc acggcgcgtg gctcggctcg gtcgtcttcg acggctcccg cgcgttcgaa
     1561 ggcgtgacgc cggatctcga ttttcatttt gagcgcgtca atgagtcggc acggcgactt
     1621 ctgctgaaac catccgtatc ggtcgaaagc tggcttgacc tcgccggcga cgggttgaag
     1681 cgcttcgatg cgaaagccga actctatatc cgcccgatgt attgggccga ccttggcacg
     1741 accggcggcg gcgtgcgctt cgatcctgat tcgacccgct ggtgtctctg cctttacgag
     1801 gcagccatgc cgaagccgag cggcttatct atcacgcttt cgccattccg gcgtccaacg
     1861 ctcgaatgcg cgccggtcga cgccaaggca agctgcaatt accccaataa cgcgcgggct
     1921 ctgatggagg cgcatcggcg cggcttcgac aattgtctga tgcgcgacat gctcggcgcg
     1981 attgccgaac tcggtaatgc caacgtcttc atggccaaag acggaatcgt ctacacgccc
     2041 gcgccgaacg gcacctttct cgacggcatc acacggcagc gtgtgagtgg cctgttgcgc
     2101 gatgctggcg tgagcgtgat cgagaaaacg ctcagctacg cggacttcta acaggccgac
     2161 gaaatctttt cgaccggaaa tttctcaa
//
LOCUS       DASWOG010000547         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_123100,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000547 DASWOG010000000
VERSION     DASWOG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123100"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VGB93_11245"
     CDS             complement(<1..929)
                     /locus_tag="VGB93_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0146699.1"
                     /translation="MNVRPASPPAYGTLTTLRSPLTRLAQLAQTIRGRILTAFLAMSI
                     ITAALGGYAALGIRRAGVLVTKTFDESLMSINYARAASADFADMQVAFARRLSATKPE
                     TRTKLDRTISDLEENLTEDLQIAAERSQSLRAARAAAEVQNAADTWSEARRRLPENIS
                     FNAAWGELDPYAETVSQQIDLLVNYTAGDGFTYRQSARQQVAVATELALVGTIAALVL
                     SGLVVLLLSRRITKPVAAASAVAKRIASGELEGEIPVGSADELGALLAAMRVMRDNIR
                     AMMEREVAQRRTAQVRLADALESSREGIVVVDA"
     gene            1098..1466
                     /locus_tag="VGB93_11250"
     CDS             1098..1466
                     /locus_tag="VGB93_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY0146700.1"
                     /translation="MTRRVMMSGKQVRLSMAVLAAAVVLTAGGTARTQQGPTPYVISQ
                     RGRAFNPNFLSIKQGDTVRIVNDDGDLLHHAYVESDTFNFDSGDQLPGSKTDVVFSVR
                     GKFNVLCGIHPKMKLIVVVK"
BASE COUNT          362 a          480 c          522 g          343 t
ORIGIN      
        1 tcggcatcaa cgacgacaat gccttcacgc gagctttcaa gtgcatcggc gagccggacc
       61 tgggcggtcc gacgctgtgc gacctcgcgc tccatcatgg cgcggatgtt gtcgcgcatg
      121 acacgcatgg cggcgagcaa cgcgcccaac tcatcggcgc taccgaccgg aatttcacct
      181 tcgagttcac cgctggcgat ccgcttcgcg acggcggaag ccgcggcgac cggcttggtg
      241 atgcgtcgcg aaagaagcag gacgaccaag cccgacaaaa cgagcgcggc gattgtcccg
      301 accagtgcaa gttccgtcgc gacggcgacc tgctggcgcg cgctttgccg ataggtaaag
      361 ccatcgccgg ccgtataatt gacaagcaag tcaatctgct gcgacaccgt ctcggcataa
      421 gggtcgagtt caccccaggc ggcattgaaa gagatgtttt ccggcagccg gcggcgcgct
      481 tcactccagg tatcagcggc gttctgcact tccgcagcgg cgcgggccgc tcgtagggat
      541 tgcgagcgtt ccgcggcgat ctgcagatct tcagtcaggt tttcttcgag gtccgatatc
      601 gtccggtcaa gtttggtccg ggtttcgggc ttcgtcgcgc tcaagcggcg cgcgaatgcg
      661 acctgcatgt cggcgaaatc ggccgacgcc gcacgcgcat agttaatcga cattaaggat
      721 tcgtcgaagg ttttcgtgac caacacgcca gcgcggcgaa tcccgagcgc ggcataaccg
      781 ccgagcgcag ccgtgatgat gctcatcgcc agaaaggctg ttagaatgcg cccgcgaatc
      841 gtttgcgcca attgggcgag acgcgttagc ggcgagcgca gcgttgtcag cgtaccatat
      901 gccggcggcg atgcaggacg gacattcata aaaataactc gaattgaata gtttgttttg
      961 gggccttcgg agttaagacg aggctaaatt cctcgtcgaa ccacgcctaa aagtggtgat
     1021 tttgctttta gttaagcgat gagcgaattg gttcccggga aagttgtgtc gctgacgggg
     1081 cgacgagaag cgggtacttg acccgtaggg ttatgatgtc aggcaaacag gtccggcttt
     1141 cgatggctgt gctggcggca gccgtggtct tgaccgccgg cggtactgcg cgcacgcagc
     1201 aaggtccgac cccgtacgtg atctcgcaaa ggggacgtgc gttcaatccg aattttctca
     1261 gcataaagca gggcgatacc gtgcggatcg tgaacgacga cggtgatctg ctgcaccatg
     1321 cgtatgtcga gtccgatacg ttcaacttcg actcgggcga tcaactgcca ggcagcaaga
     1381 ccgacgtcgt gttttcagtc cgcggcaagt ttaacgtcct ctgcggcatt catccgaaaa
     1441 tgaagctcat cgtcgtcgtc aagtgacgat gccctaaaaa agaagctccg aggatttctc
     1501 ggagcttctt gcaggaccgg ttttgcgcgt gggctcaggc gatatgcgtt ccgtagagca
     1561 ccgaatgcag cacgtaatag aacgccagga aggctgcgag ggcggcgcaa taggcagccg
     1621 ggatcagaac gatatccccg ctcgacatcg ccaatttgaa cttggcaaga ttttccttga
     1681 tgcaaagaca cacatgaatc ggccgcc
//
LOCUS       DASWOG010000548         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_159544,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000548 DASWOG010000000
VERSION     DASWOG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159544"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGB93_11255"
     CDS             complement(<1..576)
                     /locus_tag="VGB93_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0146701.1"
                     /translation="MDINRKVLVVDGDPQSRQLKVQLLANAGLRLTAVETGREALKSL
                     DAEIFDLVVANDEAADGDEVAFYQNVKEKSPSTAVLRMAHATTVKTSAAIDAEIDGFL
                     LDPFEPSELITLVRSLLRLNRARLALRDAEARLQLVQDTGGLAVLDCDLVTRRALWSE
                     KFAQIFSLPPDTSAKRLRFGMILNFVHPDDRV"
     gene            complement(747..1553)
                     /locus_tag="VGB93_11260"
     CDS             complement(747..1553)
                     /locus_tag="VGB93_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEY0146702.1"
                     /translation="MFSASEVELYARHLVFRDVGGPGQQKLKAARVLVIGAGGLGSPV
                     IQYLAAAGIGEIGILDDDVVSLSNLQRQVIFTTDDIGKRKTDCAAAAVQRLNPHVKVA
                     PLAQRLDVENAREIVAGWDVVADGSDNFATRYAVSDACFYEGRPLVTAAIGTFDGSLT
                     TLRPFEMSQSGAPNPTYRCLFPEPPPPGSVPTCEETGVIGALAGTLGSLMAHEIIREI
                     VGFGEGLVGRLMLVDMAHMRFESIAYGWDRDNPLNGITAKPKPVGADLGF"
BASE COUNT          325 a          460 c          495 g          321 t
ORIGIN      
        1 gacgcggtcg tccggatgca cgaaattcag gatcatgccg aagcgcagcc gttttgcgga
       61 ggtgtccggc ggcaaggaaa atatctgcgc aaacttctcc gaccaaagcg cgcgacgcgt
      121 cacgagatcg caatcgagca cggcgaggcc gccggtgtcc tggacaagtt gtagcctggc
      181 ttcggcgtcg cggagcgcta ggcgcgcgcg gttcagccgc aaaagcgagc gaaccagcgt
      241 gatgagttcc gacggttcga aggggtcgag gagaaatccg tcgatctctg cgtcgattgc
      301 cgcagaggtt ttcaccgtgg tcgcgtgcgc catgcgcagc accgctgtcg acgggctttt
      361 ctctttcacg ttttggtaga aagcgacttc gtctccgtcg gcagcctcat cgttcgcgac
      421 gacgagatcg aagatttccg cgtcgagaga tttgagcgcc tcgcggccgg tttcgacggc
      481 ggtgagacgc agaccggcgt tggccagaag ctggaccttc aattggcgag attgcggatc
      541 gccatcgaca accagcactt ttcgattgat gtccatctgg atggcgacct atgtccgcgt
      601 tacatcgaga ttgccggccg gctcgtgacg cactcgtgcg aagctatgat tgagcgggaa
      661 cttggttcca cgtctttgca catattcggc gcatattgct tgccggactc ataatccgac
      721 cgtcgccaag atgaaagcga gagcattcag aagccgagat cagcgccgac aggctttggc
      781 ttagcggtga tgccgttgag cggattgtcc ctgtcccaac cgtaagcgat gctttcgaag
      841 cgcatatgcg ccatgtcgac gagcattaaa cggccgacga ggccttcgcc gaagccaacg
      901 atttcgcgaa tgatttcatg tgccatcaaa cttccgagcg taccagcgag cgcgccgatc
      961 acgcccgttt cctcgcaggt tggcacgctg ccgggcggtg gcggttctgg gaagaggcaa
     1021 cgataggtcg ggttcggcgc gccagattgt gacatctcga agggccgcag cgtcgtcaac
     1081 gaaccgtcga atgttccgat ggcggcagtg acaagcgggc gaccttcgta aaagcaggca
     1141 tcggagactg cataacgggt cgcgaaatta tcagagccgt cggcgacaac atcccatccg
     1201 gcgacgatct cgcgcgcgtt ctcgacgtca aggcgttgcg cgagcggcgc gactttgacg
     1261 tgcggattaa gccgctggac agcggctgcg gcgcaatcgg tcttgcgttt accgatgtcg
     1321 tcggtcgtga agatcacttg tctttgcagg ttcgacagcg agacgacatc gtcgtcaagg
     1381 atgccgattt cgccgatgcc tgccgccgcg agatattgga tgaccgggct accgaggccg
     1441 cccgccccga tgacgagcac gcgcgcggcc ttcaatttct gctgaccggg tcctcccaca
     1501 tcgcgaaaca caagatggcg cgcgtaaagt tcgacttcac tcgctgaaaa catgggctct
     1561 atctaatacc gcggagcgca aaaatcgatt ccgtccgtgt a
//
LOCUS       DASWOG010000549         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_105445,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000549 DASWOG010000000
VERSION     DASWOG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105445"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VGB93_11265"
     CDS             <1..854
                     /locus_tag="VGB93_11265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0146703.1"
                     /translation="ENVSDTAFWVAHYRAKESARPDALFHDPLADRLAGTRGEEIARA
                     IPGRVMTGWIVSLRTCIIDDFLRFAIDQGVDLVLNLGAGLDTRPYRLDFIDKVTWVEV
                     DYPAMIAYKDEQLAGEAPRCQLERVKLDLADTAARQKFLAEMNARSKNILVLTEGVLP
                     YLSNDEVGALADDLRRQENIRFWIADYMAAQIQRFRRRLSRRHLRNAAFKFAPGDWFE
                     FFARRGWQSKTMRYYLEESIKLNRPITLPLPYRLVMGVRWLFATRQQRENFGKFAGFA
                     LLVPKDR"
     gene            complement(856..2964)
                     /gene="ligA"
                     /locus_tag="VGB93_11270"
     CDS             complement(856..2964)
                     /gene="ligA"
                     /locus_tag="VGB93_11270"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0146704.1"
                     /translation="MTKSKTSPTADNADAQAEYVRLGAEIAHHDALYYREDAPEISDA
                     DYDALRWRYEALEKAHPEIVTEKSLSQRVGAAPSEKFAKVRHRVPVLSLGNVFADSDV
                     EEFVARVRRFLGLAADSPLAMTAEPKIDGLSCSLRYERGRLVLAATRGDGYEGEDVTA
                     NVKTIHEIPHILKHDPPDVLEVRGEIYMTRVDFTALNVRQAEAGKALFANPRNSAAGS
                     LRQLDPAITASRPLHFFAYAWGEVSKMPAATQMGMIEKFKRYGFTVNLLTALCHSAAD
                     LLRHYHDIESRRASLGYDIDGVVYKVDDLALQDRLGFVSRAPRWAVAHKFPAEQATTV
                     LRDIEIQVGRTGAMTPVARLDPVNVGGVIVANATLHNEDEIARKDIRVGDTVRLQRAG
                     DVIPQIVGVILAKRPTGSEPYKFPHKCPVCGSAAVREIDPKTGRADVVRRCTGGLICA
                     AQVKERLKHFVSRLAFDIEGLGEKQIDQFYAEGLITKPDDIFTLAARDARSLTKLANR
                     EGYGQTSVRKLFAAIEARREIALNRFIFALGIRHVGETNARRLARHFGTFEALRQTAR
                     VASEGSDARAEIDNIEGLGAVVAEAVADFFGEEHNEVELDALLRYVTVTPMEAVASSS
                     PIAGKTIVFTGSLERMTRDEAKAQAERLGGKVAASVSKNTDLVVAGPGAGSKLAKAKE
                     FDVETIDEDTWLKLVADARD"
     gene            complement(3053..3889)
                     /locus_tag="VGB93_11275"
     CDS             complement(3053..3889)
                     /locus_tag="VGB93_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0146705.1"
                     /translation="MIFSNISQPRIRGVVTAIWQLGALAFCGAVSAADLPTEKAPVFA
                     PPPPAFSWTGLYAGVNGGIALDHYAFVYQYFPTSVPGGGGSNGITSTGPVIGGQVGYN
                     YELSDQSISGLSFLNHAVIGIEADSDWGDVKGWTNVSAAGRTLHIGTDFENFGTLRLR
                     LGYSFDRLLVYLTGGLSYATVDNYYNVAGVSVSRTQTHAGVFPNFGVVGIGAEYAITD
                     HFSVKAEYLYDFNGARWDQLNSPTTGIVGFGTRAMYHIARLGLNYKFESFGARRSIQN
                     IE"
     gene            complement(4086..5756)
                     /gene="recN"
                     /locus_tag="VGB93_11280"
     CDS             complement(4086..5756)
                     /gene="recN"
                     /locus_tag="VGB93_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY0146706.1"
                     /translation="MLAQLAIRDIVLIEKLDLEFDRGLTVLTGETGAGKSILLDAFSL
                     ALGARGDGTLVRQGAGQGQVTAVFDLPSGHPGLMAAEAQDINIDGALILRRVQMGDGR
                     TRAFVNDQPVSAQALRAIARELVEIHGQHDDRALVNADAHRQLLDAFGGLGGERDAVR
                     AAYKALRAAEAVYAAEESRIEQARREADYLTHAHAELTKLAAVPGEEEALAERRQVLM
                     QAEKVSGELRDAAEAIGGDGAPMSAIASALRRLERRRSQAPDLLALAVNALDAALSAF
                     DAARQALDEALRVAGDNPGELEQVEERLFSLRAASRKFGVAVEALPALKDKIGNDLSR
                     LEAGESRLVRLRETVVSAAAAYNGAAKALSAARQSAAREIERAVNIELPPLKLETAEF
                     IVKLQTDAAAGTSEGIDQVEFWVKTNPGTRPGPLMKVASGGELARFMLALKVVLADRG
                     SAPTLVFDEIDTGAGGAVADAIGQRLARLAGGIQVLAVTHAPQVAAQAGGHFRITKDA
                     AGEGERVATRVLTLTKAARREEIARMLAGATITAEARAAASRLMNSRG"
BASE COUNT         1165 a         1779 c         1691 g         1146 t
ORIGIN      
        1 ttgaaaacgt ctcggatacg gccttctggg ttgcgcatta tcgcgccaag gaaagcgcgc
       61 gacctgatgc gctcttccat gatccgctcg ccgaccgcct cgcgggcacg cgcggcgaag
      121 agatcgcccg cgctattccc ggtcgggtga tgacgggctg gatcgtttcg ctccgcactt
      181 gcatcatcga tgattttctg cgtttcgcca tcgatcaagg tgtcgacctt gtgctcaatc
      241 ttggcgccgg cctcgacacc cgaccctatc ggttggattt catcgataag gtcacatggg
      301 tcgaagtcga ttatccggcg atgatcgcct acaaagatga gcagcttgcc ggcgaggcac
      361 cccgctgcca gctcgaacgg gtcaaactcg acctcgcgga tacagcggcg cgacagaagt
      421 tccttgctga gatgaacgca cgcagcaaaa atattctggt gctgacggaa ggcgtgctgc
      481 catatctcag caatgacgag gtcggcgcgc ttgccgacga tttgcgcaga caggaaaata
      541 tccgtttctg gatcgccgat tatatggccg cgcagattca aagattccgg cggcggctgt
      601 cgcgacggca tttacgcaat gctgcgttca aattcgcacc gggcgattgg ttcgaatttt
      661 tcgcgcggcg cggctggcaa agcaaaacga tgcgttacta tctcgaagaa tcgatcaagc
      721 taaaccggcc gattacactg cccttgccct atcgactcgt gatgggcgtg cgttggcttt
      781 tcgcgacgcg ccagcagcga gagaatttcg gcaaattcgc gggttttgcg ttgctggttc
      841 ccaaggatcg gtagttcaat cgcgcgcgtc ggcgacgagc ttcaaccacg tatcttcgtc
      901 aatcgtctcg acgtcgaatt cttttgcctt ggcgagcttt gagcccgcgc ccggcccggc
      961 cacgacgaga tcggtattct tcgagacgga cgccgcaacc ttaccaccga gacgttcagc
     1021 ctgcgccttg gcttcatcac gcgtcatgcg ttcaagcgag ccggtgaaca cgatcgtctt
     1081 gcccgcgatg ggacttgatg aagcgaccgc ctccatcggc gtcaccgtga catagcgaag
     1141 cagcgcatca agctcgactt cgttatgttc ttcgccgaag aaatctgcga cggcttccgc
     1201 aacgaccgcg ccaagccctt cgatattgtc gatttccgcg cgcgcgtcag agccctcgct
     1261 cgcgacacgc gctgtctgac gtaacgcctc aaacgtgccg aaatggcgcg cgaggcgacg
     1321 cgcgttggtc tcgccgacat ggcgaatgcc gagggcgaaa atgaaacggt tcagcgcaat
     1381 ctcgcgccgc gcctcgatgg cggcaaaaag tttacgcacc gaggtctgac catagccctc
     1441 gcgattggcg agtttcgtca ggcttctcgc gtcacgtgcc gctagtgtaa aaatatcatc
     1501 gggctttgta atcagccctt cggcatagaa ttgatcgatc tgcttttcgc caaggccttc
     1561 gatgtcgaag gcaaggcgcg agacgaaatg cttgaggcgt tccttcacct gcgccgcgca
     1621 aatcagacct ccggtgcagc gacggacaac atcagcgcgg ccggttttag gatcgatttc
     1681 gcgaacggcc gccgagccgc acacaggaca tttgtgtggg aacttatatg gctctgaacc
     1741 tgtcggccgc ttcgccagaa taacaccgac gatttgtggg atgacatcgc cggcgcgctg
     1801 caaccgtacg gtatcgccga cgcgaatgtc tttccgcgcg atctcgtcct cgttgtgcag
     1861 tgtcgcattg gcgacgatga cgccaccgac gttgaccgga tcgagccgtg cgacaggtgt
     1921 catagcgccg gtgcggccga cttgaatttc gatatcgcgc aggacagttg tcgcctgctc
     1981 ggccggaaat ttatgcgcaa ccgcccaacg cggcgcgcgc gagacgaagc ccagccgatc
     2041 ctgcaaagcg agatcgtcga ccttatagac gacaccgtcg atgtcgtagc caagcgaagc
     2101 ccggcggctt tcgatgtcat gataatggcg caataagtct gctgctgaat gacagagcgc
     2161 ggtaagcaga ttgaccgtga acccatatcg cttgaatttc tcgatcattc ccatctgcgt
     2221 cgcggccggc atcttgctca cctcgcccca ggcgtaggca aagaagtgca acggccgcga
     2281 tgcggtgatc gcaggatcga gctgccgcaa cgagcccgct gcggaattac gcggattggc
     2341 gaagagcgct ttgcctgctt ccgcctggcg cacgttgagc gcggtgaaat caacgcgcgt
     2401 catatagatt tcgccgcgca cttcaagcac gtccggcgga tcgtgcttca agatatgcgg
     2461 tatctcgtgg atggttttga cgtttgccgt gacgtcctcg ccctcatagc catcgccgcg
     2521 tgttgccgcg aggacaagcc ggccgcgctc atagcgcagg gaacaggaaa ggccgtcaat
     2581 cttgggctca gccgtcatcg ccagcggcga gtcagcggca agtccaagga aacgccggac
     2641 gcgtgcaacg aattcttcga catcggaatc ggcgaaaaca tttccgagcg agagcaccgg
     2701 cactctatgc cggaccttgg cgaatttttc tgacggcgcc gcgccgacgc gttggctcag
     2761 cgatttctcg gtgacgattt cgggatgggc cttttccagc gcctcatagc gccagcgcaa
     2821 cgcgtcataa tccgcgtcgg aaatttcggg cgcatcctcg cgataataaa gcgcgtcatg
     2881 atgcgcgatt tccgcaccga gacggacata ttccgcttgt gcgtccgcgt tgtcggcagt
     2941 gggggatgtc tttgacttgg tcacgtcgaa aatttagcgc atcgcgaccg aaagaggaaa
     3001 ccgatttcac gctggtgctg cgaattcgac gataagcttg atcgtgtctg cttcattcaa
     3061 tattttgaat cgaccgccgc gccccaaagg actcaaattt ataattcaaa ccgaggcgcg
     3121 cgatgtgata catcgcgcgc gtgccgaagc caacgatacc cgtggtggga ctattaagct
     3181 gatcccaacg tgcgccgttg aaatcgtaca gatactccgc tttcaccgaa aaatggtcgg
     3241 tgatcgcgta ttcagcgccg atgccgacaa caccgaaatt tggaaatacg ccggcgtgtg
     3301 tctgcgtccg ggagaccgaa acacctgcga cgttgtaata attatcgaca gtcgcatagc
     3361 tgagaccgcc ggtcagatag acgagcaatc gatcgaaaga atatcccagc cgcaaacgca
     3421 gcgtcccgaa attctcgaag tctgttccga tgtgaagcgt gcggccggcc gcggagacat
     3481 ttgtccatcc cttcacgtcg ccccaatccg aatcggcttc gatgccgatc acagcatgat
     3541 tcaggaaaga cagacctgag atcgattggt ctgagagttc ataattgtaa ccgacctggc
     3601 caccgataac ggggccagtc gaggtaatgc cgtttgagcc cccgccgccc gggacactag
     3661 tgggaaagta ttgatagaca aaggcgtaat gatcgagcgc gataccgccg ttcacacccg
     3721 cataaaggcc cgtccagctg aacgctggcg gcggcggtgc gaagaccggc gctttttcgg
     3781 tcggcaagtc ggcggcggat accgcaccgc agaaagcaag tgcgccaagc tgccaaatgg
     3841 cggtgacgac gccgcgaatt ctcggctgag agatgtttga aaaaatcatc aatcacttgc
     3901 tcaaattcgg aagaccacaa tgccgcacct tcagggcgtt gcaagcctaa gcgtacccgg
     3961 gcgttgaaat ttgctgtcga gtggcattcg cgcagcactt atctttgacc ccagtgtcat
     4021 gcgcggcgcg acggtcgaag agctccacga tcgataagct ttcggaaaat cgttggattt
     4081 tcgattcagc cgcggctgtt catcagccgg ctggcggccg cccgggcttc ggcggtgatt
     4141 gttgcacctg cgagcatccg cgcaatttct tcacgacgtg cggctttcgt gagagtcaaa
     4201 acgcgggtcg cgacgcgctc accctccccg gccgcatcct tggtgattcg aaaatgcccg
     4261 ccggcttgcg ccgccacttg cggcgcatga gtcacggcca gtacctggat gccgccggca
     4321 agccgagcaa gtctttggcc gatcgcatct gcgacagccc cgcccgcgcc ggtgtcaatc
     4381 tcgtcgaaga cgagcgtcgg cgccgagccg cgatctgcca ggacgacttt gagtgccaac
     4441 atgaagcgcg ccaattcacc gcctgatgcg accttcatca gcggcccagg acgcgtacca
     4501 gggttggtct tcacccagaa ttcgacttga tcgattccct cagaagttcc agcggccgca
     4561 tccgtctgca acttcacgat gaattcggca gtttccaatt ttaacggcgg caattcgata
     4621 ttgacggcac gttcgatctc gcgcgcggca ctctggcgcg ccgcagatag agccttggcc
     4681 gcgccgttat aggccgctgc cgcgcttacg accgtttcgc gaagccggac cagccgggat
     4741 tcgccggctt caagccgcga aagatcgttg ccgattttgt ccttcaacgc cggcaaggct
     4801 tcgaccgcaa cgccgaattt gcggctcgcc gcgcgtagcg aaaacagcct ctcctcgacc
     4861 tgctccaatt cgcctggatt atctcccgcc acgcgcaacg cttcgtcgag cgcctggcgt
     4921 gcggcgtcga aggcggacag cgccgcatcg agggcattga cggccagcgc caacaggtcc
     4981 ggcgcctgcg accgccgccg ctccagccgc cgcaaggccg aggctatggc gctcatcggc
     5041 gcgccatctc cgccgatcgc ctcagcggcg tcgcgcaatt cgccgctgac cttttcggcc
     5101 tgcatcaaaa cctgccggcg ctcggcaagt gcctcctctt cgccgggcac ggcggcaagc
     5161 ttggtcaatt cggcatgcgc gtgggtcaga taatcagcct cgcgccgcgc ctgttcgatg
     5221 cgcgattctt ccgccgcata gacggcttcc gccgcgcgta gggctttgta ggcggcgcgc
     5281 accgcgtcgc gctcgccgcc aaggccgccg aacgcatcaa gcaattggcg gtgcgcgtcg
     5341 gcgttgacga gggcgcggtc gtcatgctgg ccgtggatct caacaagctc gcgcgcaatt
     5401 gcacgcagcg cctgggcgct gaccggctga tcattgacga acgcgcgggt gcgcccatct
     5461 cccatctgga cacggcgcag gatcagcgcc ccgtcaatat ttatgtcctg cgcctccgcg
     5521 gccatcaagc caggatggcc ggagggcaga tcgaacaccg cagtaacctg cccctggcct
     5581 gccccctggc ggacgagcgt gccatcgccc cgcgcgccga gcgcgaggga aaaagcatcg
     5641 agcaggatgg atttgccggc gccggtctcg ccggtgagca ccgtcaggcc gcgatcgaac
     5701 tcaagatcga gcttttcgat cagcacgatg tcgcggatag cgagctgagc gagcataagg
     5761 caccctacgc ggacagcgga c
//
LOCUS       DASWOG010000550         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_49452,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000550 DASWOG010000000
VERSION     DASWOG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49452"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VGB93_11285"
     CDS             complement(<1..643)
                     /locus_tag="VGB93_11285"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEY0146707.1"
                     /translation="MSLKASLADLRRFYGIDAECAAILRRNRDFLSREFTAALDLFYR
                     TAPAILGISSYINDPASVAFAREKYLRHWETMMQARFADDYADSVASIYRLRNDFGIN
                     LQLSMGGRSFILARVLEAIALRLPRKPWDFSARQRRAKLQLAYLRVTMLDLLITMDGY
                     LEAGQDERTRTLSHLAKSFEHAVGDIVAGVTAAAGDLRSTSDVLTLSAEETTKQ"
     gene            825..2000
                     /gene="rmuC"
                     /locus_tag="VGB93_11290"
     CDS             825..2000
                     /gene="rmuC"
                     /locus_tag="VGB93_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0146708.1"
                     /translation="MNQILFVAFGTPVSVAILAMGLSGLAFVLLVLTLSLGANGRRRD
                     AAEAVARQVDLQEKLADLARAHAEVTGRVRSMGEILGSRQADLARFVAEQLESVGSRV
                     GRSLEDQSRNSTESLGKLNERLAVIDAAQARLTGLTEEVIGLKDILANKQARGAFGQG
                     RMEAIVRDALPGNAYEFQYTLSNRCRPDCVIRLPGDDRVMVVDAKFPLEAFVALKDAP
                     DAEARKIAATRVRADVGRHIAYIKELYFVAVETQDIAILFVPSESLYADLAEHFEDVV
                     QKAHTNRIVIVSPSLLVMAVQVMQALVRDARVREQAHIIQVEVRRLVEDVVRLRQRVG
                     KLSAHFGHAQEDIAGIVISADKIAKSGERIDMLDFEQAPSPNAQAEFALGGLGKAAE"
     gene            complement(2273..>2793)
                     /locus_tag="VGB93_11295"
     CDS             complement(2273..>2793)
                     /locus_tag="VGB93_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0146709.1"
                     /translation="IGGFGPEANDRLVYENDVNVTWTATDKITLIGEANYTKNDLTGS
                     STYGAVGYVSYQTQWDWLKINGRAEVFRDNNGGFVNAYPGNFDFVDFEHGYPNTAVLG
                     PPATYLELTAGLNITPTLPTSIPFVKSVIFRPEVRYDSTLNGATPFDLDAFDVGHKTD
                     QVTIGGDVIVKF"
BASE COUNT          582 a          773 c          863 g          575 t
ORIGIN      
        1 attgctttgt cgtttcctcg gcagacaagg tcagcacatc cgaagtgctg cgcaaatcgc
       61 cggcggcggc tgtgacaccg gcaacaatat caccgaccgc atgttcgaag gatttggcga
      121 gatggctgag cgtgcgggtt cgctcgtcct ggccggcttc gagataaccg tccatcgtga
      181 tgagcaaatc cagcatggtg acgcgcaaat aggcgagttg gagcttcgcc cggcgttgcc
      241 gcgcggagaa atcccagggt ttgcggggca aacgcaaagc aatggcttcg agaacgcgcg
      301 ccagaatgaa actgcggccc cccattgaga gctgaagatt gattccgaaa tcgttgcgta
      361 ggcgatagat gcttgcgacc gaatccgcgt aatcgtctgc gaatcgcgcc tgcatcatag
      421 tttcccagtg acgcagatat ttttcgcgtg cgaatgcgac gctggccgga tcgttaatgt
      481 aggacgatat ccccagaatc gctggcgcgg ttctatagaa caggtcgagc gctgcggtga
      541 attccctcga cagaaaatcg cgattgcgcc ggaggatcgc ggcgcattcc gcatcgatcc
      601 cgtaaaatcg ccgaaggtcg gcgagcgaag ctttcaaaga catctgcact tccatctgga
      661 gcgcaccaac tctaggagga ttttattaag cgagctgtaa cgcagtgcga aaaccctgcc
      721 gaaaccgcat cttgcgatgt aggtgccgca atcaagcgtc gcttgtcctc gaaaatatgt
      781 tccgccttcg ttcacacgca ttccgtgatc tggtaggaag gcgtatgaac cagattctct
      841 tcgttgcatt tggaacgccg gtttccgtcg cgatattggc gatgggcctc tccggattgg
      901 cgtttgttct gctcgtcttg acgctgagtc ttggcgcaaa tgggcgtcgc cgcgacgcag
      961 ccgaggcggt ggcgcgacag gtggatttgc aggaaaagct cgccgacctt gcacgcgctc
     1021 acgcggaagt gacaggacgc gtgcgcagca tgggcgagat tctcggttcg cggcaggcag
     1081 acctcgcgcg ctttgttgcc gagcagctcg aatcggttgg ctcgcgcgtc ggccgcagtc
     1141 ttgaagatca atcgcgcaat tcaaccgaga gcctcggcaa gctcaacgag cgtctggcgg
     1201 tgatcgacgc ggcacaggcg cgccttacgg gcctgaccga ggaagtgatc ggactcaaag
     1261 acattctcgc aaacaagcag gcgcgcggtg cgttcggcca gggacggatg gaggcgatcg
     1321 tccgcgatgc gctgccaggg aacgcctacg aatttcaata cacgttgtcg aaccgctgcc
     1381 gcccggattg cgtgattcgc ctgccgggcg atgatcgggt gatggtggtc gacgccaaat
     1441 ttccgctcga agctttcgtc gctttgaaag acgcgccgga cgccgaggcg cgaaagattg
     1501 ccgccacgcg ggttcgagct gatgttggcc ggcatatcgc gtatatcaaa gaactttatt
     1561 tcgtcgccgt cgaaacacag gacattgcca ttctcttcgt gccttcggaa tcgctctacg
     1621 ccgacctcgc tgaacatttc gaggacgtcg tgcagaaggc gcataccaac cgtatcgtga
     1681 tcgtctcgcc gtcgctgctc gtgatggccg tgcaggtgat gcaggcgctg gttcgcgatg
     1741 cgcgcgtgcg cgaacaggcg catatcatcc aggtcgaagt gcggcggctg gttgaggatg
     1801 tcgtgcggct gcgccaaagg gtcggcaagc tcagcgccca ttttggccac gcgcaagagg
     1861 atattgccgg cattgttatt tctgccgata agatcgccaa aagcggcgag cgcatcgata
     1921 tgctcgactt tgagcaagcg ccatcgccaa acgcgcaggc ggagttcgcg ctgggggggc
     1981 tcggcaaagc cgcggagtaa agccgggcag attgcggcgc tttttgcgtg gccggggtcg
     2041 cgtaaattgc ctcgtaacct aaagcaggcg ctcatccgat agtgcggaac gggacgatcc
     2101 tcgtatttgg cagcccggaa ccgtcgcaca actgcagcaa caagcggcga acctaaccta
     2161 gcgcctcgga ggactctttc aggcggcttt agctacgcgc ggcttccgca gttcgcgcaa
     2221 acaaaaacgc cggcccgaag gccggcgttt aatattgttt ctctcagaga gatcagaact
     2281 tcacgatgac gtcgccaccg atcgtgacct gatccgtctt gtggccgacg tcgaaagcgt
     2341 cgaggtcgaa gggcgtcgcg ccgttcagcg tggagtcata gcgaacttcc ggccggaaga
     2401 tgaccgactt gacgaagggg atcgacgtcg ggagcgtcgg cgtgatgtta aggccagccg
     2461 tcaattcgag ataggtggct ggcggcccga gaacagcggt gttgggatat ccatgctcga
     2521 aatcgacaaa gtcgaaattg cccggatatg cgttcacaaa accaccattg ttgtcacgaa
     2581 aaacctcagc gcggccgttg atcttcagcc agtcccattg cgtctggtaa gagacataac
     2641 cgacggcgcc ataggtggag gatccggtca gatcgttctt ggtgtagttg gcctcgccga
     2701 tgagcgtgat cttgtccgtc gcggtccagg ttacgttgac gtcgttttca tagacgagac
     2761 gatcgttcgc ttccgggcca aatccgccaa ttg
//
LOCUS       DASWOG010000551         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_361034,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000551 DASWOG010000000
VERSION     DASWOG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361034"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /gene="pheT"
                     /locus_tag="VGB93_11300"
     CDS             complement(<1..1725)
                     /gene="pheT"
                     /locus_tag="VGB93_11300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0146710.1"
                     /translation="MKFTLSWLKQHLDTNADLATIVDTLTKVGLEVEHVHDPAAALKD
                     FVIAEVLEAYPHPNADRLRLCFVEIGSGKPVQVVCGAPNARAGMKTVFAPPGAYIPAK
                     GITLSKGVIRGVESAGMLCSGAELEVSTDQEGILDLPADAPVGTAYAKWAGLDDPLIE
                     INLTPNRPDAAGVLGIARDLDAAGIGQLQTPSVEPIAGSFPCPVNVSLDLAEADRHFA
                     PAFALRLVRGVKNGPSPDWLQKQLRAVGLRPINALVDITNFLTIDQARPLHVFDAAKV
                     AGDLHVRRAKTGESFLALDGKTYNLDENAIVIADANGVESLTGIMGGEATGCTEATTD
                     VLIESALWDSVNIARTGRRLSINSDARYRFERGIDPASVLPGLELATQLVLEFCGGEA
                     SEIILAGEIPDAHKRVTFPWSEVRRLAGVELSPSEMASILEHLGFRPASLPGNSDLVI
                     IDVPSWRPDIEGKADIVEEILRIAGLDRIAAQPLPREDAHISSAILTPLQKLVRSVKR
                     ALAANGLTEAINWSFIGHEAAQVFGGGDDSLVLANPIASDLSDMRPSLLPGLIAASKR
                     NAARGFGDV"
     gene            complement(1876..2883)
                     /locus_tag="VGB93_11305"
     CDS             complement(1876..2883)
                     /locus_tag="VGB93_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY0146711.1"
                     /translation="MNEALRRADRLVTVFGGAGFLGRHVVRALAKDGWLVRVAVRRPD
                     LAFHLQPSGNVGQITAVQANLRYPDSIAAALRNADAAVNLVGILREQRRQTFDAIHRE
                     GAANIAAAVRDAGIEHFVHVSALGADPNSASAYGRSKAAGEAAVQAALPGAIILRPSV
                     VFGPEDHFFNRLAALARLSPVVPLIGGGVTKLQPVYAGDVAKAVAAGVRGSAAGIIYE
                     LGGPEVRSLREIIEFILAETERKRFLMSLPFGPARGFGGMSELVAKLTLGLMPEDFVL
                     TTDQVELLQQDNVVSTAASTERRTLNGLGIAAESYEVLVPTYLTRYRKTGLFGTNDAF
                     G"
     gene            3038..3122
                     /locus_tag="VGB93_11310"
     tRNA            3038..3122
                     /locus_tag="VGB93_11310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3072..3074,aa:Leu,seq:cag)
BASE COUNT          655 a         1015 c         1071 g          603 t
ORIGIN      
        1 gacatcgccg aagccccgcg cggcattgcg cttcgacgcg gcgatgaggc ccggcaagag
       61 gctcggccgc atatcggaaa gatcggacgc gatcggattg gcgagcacga gagaatcgtc
      121 gccgccgccg aagacttgcg ccgcttcatg tccgatgaaa gaccaattga tcgcttcggt
      181 gaggccgttg gcagcaagcg cgcgtttgac gctgcgcacg agcttttgca gcggcgtcag
      241 aatcgcggaa gaaatatgcg cgtcctcgcg cggcagaggt tgcgcggcga tcctgtcgag
      301 gccggcaatg cgcaggattt cctcgacgat atcggccttg ccttcaatat ccggacgcca
      361 cgacggaacg tcgatgatga cgaggtcgct gttgccgggc aggctcgcag gacgaaagcc
      421 gagatgttcg aggatcgacg ccatttccga tggcgacagt tcgacaccgg cgagacgccg
      481 cacttcgctc cacggaaacg tcacgcgttt gtgcgcgtcc gggatttccc cagcgagaat
      541 gatttcggag gcttcaccac cgcagaactc gagcacgagc tgcgtcgcaa gttcgagtcc
      601 cggcaaaacg gaggccggat cgataccgcg ctcgaaacga tagcgcgcat cggaattgat
      661 actgaggcgc cgtccggtgc gggcgatatt taccgagtcc cacaacgccg attcgatcag
      721 cacatcggtc gtcgcctcgg tacagcccgt cgcttcgcca cccatgatgc cggtgagcga
      781 ttcgacgcca ttggcatcgg cgatgacaat ggcattttca tcgagattgt aggttttgcc
      841 gtcgagcgcg aggaagctct cgcccgtttt ggcgcggcgt acatgcaaat cgcctgcgac
      901 tttcgcggca tcgaacacat gcagcggccg cgcctggtcg atcgtcagaa agttcgtgat
      961 gtcgacaagc gcgttgatcg gccgaagccc gacagcgcgc aactgctttt gcagccagtc
     1021 aggactcggc ccgttcttga cgcctcgcac aagccgcagt gcaaaagccg gtgcgaaatg
     1081 ccggtcggcc tcggcaagat cgaggctgac gttcacggga cagggaaagc tgcccgcgat
     1141 cggttcgacg gacggtgtct gcaattggcc gatcccggcg gcatcgagat cgcgcgcgat
     1201 gccgagcacg cccgcggcgt ccggtctgtt gggcgtcaaa ttgatttcga tcagcggatc
     1261 gtcaaggccc gcccatttgg cgtaggcggt gccgacaggc gcatccgccg gaagatcaag
     1321 gatgccctcc tgatcggtcg agacttcaag ctctgccccc gaacagagca tgccggcgga
     1381 ttcgacgccg cggatcacgc ctttgctcag cgtgatgcct ttggcgggaa tataggcgcc
     1441 gggcggcgcg aagaccgtct tcatgccggc gcgcgcgttg ggggcgccgc agacgacctg
     1501 caccggcttg ccgctgccga tctcgacgaa acacaggcgc agtcggtcgg cattcggatg
     1561 cggatatgcc tccagaactt cggcgatgac gaaatccttc agcgccgcag ccggatcgtg
     1621 gacgtgttcg acctcaaggc cgactttcgt caaagtgtcg acgatggtcg cgagatcggc
     1681 gttagtatcg agatgctgct tgagccagct tagggtgaat ttcattgaga gtccggttgc
     1741 gggcgcccgc gagccacgcc tccggcgccc acggcgtcgc cggtatagag acagaaggcg
     1801 gcgaattcca gcctagggcg ttttcaaccg aagcggacgc cggatcgcgt gaagaaagcg
     1861 cgctaaaaca aggccttagc cgaaggcatc gttggtgcca aacaggcccg ttttgcgata
     1921 gcgcgtgaga taggtcggca ccagcacctc gtaggattcg gcggcgatgc cgaggccgtt
     1981 caaggtccgc cgctcggttg aagccgctgt ggagaccaca ttatcctgct gcagcaattc
     2041 gacctgatcc gtcgtgagca caaagtcttc gggcatcagg cccaatgtca gtttcgccac
     2101 caactcgctc atgccgccga agccccgcgc ggggccaaaa ggcagcgaca tcaggaaccg
     2161 cttgcgctcg gtctcggcga ggatgaattc gatgatctcg cgcaggctgc gcacttcggg
     2221 gccgccgagt tcgtaaatga tccccgccgc actgcccctg acgccggcgg ccaccgcctt
     2281 ggcgacatcg ccggcataga ccggttgcag cttagtgacg ccgccgccga tcagcggcac
     2341 caccggcgag agacgcgcca gcgcggcgag acgattgaag aaatgatctt ccggcccaaa
     2401 cacgacggac ggccgcagga tgatcgcgcc gggcagcgcc gcctgcaccg ccgcttcgcc
     2461 agccgccttg ctgcggccat aggccgaggc cgaattggga tcggcgccga gcgcggacac
     2521 gtggacgaaa tgttcaattc ctgcatcgcg aaccgcagcg gcgatattgg ctgcgccctc
     2581 gcggtggatc gcgtcgaaag tctggcgccg ctgctcgcgc agaatgccga cgagattgac
     2641 tgctgcatcg gcattgcgca gggcggctgc aatcgagtcg ggataacgca gattggcctg
     2701 aacagcggtg atctggccaa cgttgcctga cggctgaaga tggaaggcca ggtccggccg
     2761 gcgcaccgcg acgcgaacga gccagccgtc tttggccaag gcgcggacga catgacggcc
     2821 caagaatcca gcaccgccaa agaccgtcac cagccggtcg gcgcgtcgca aagcttcgtt
     2881 catcgctgag acccccgtgg cgcttgctgg ccgcattttt aatccagccg gggcaaatct
     2941 gtatagggca cggcggctgg aaatcgcctt tttcgcactt gcggcggggc gccgattgac
     3001 aagggggcga actggcccct atgggatggg accgcatgcc ctggtggcgg aattggtaga
     3061 cgcgctggtt tcaggtacca gtggtgaaag ccgtggaggt tcgagtcctc tccagggcac
     3121 cagcttctaa gctgttgtaa tatatagcag agattgcttt cggtatctca ccggcaaaaa
     3181 acaaggcgat cttccttgaa ttcccggtcc caattaggct tcctgtggaa gatttaggcg
     3241 tcaccgcttt tcgcaattgc atttacgagg cgctcatcgg gcggcgcgat ttcggtcaca
     3301 agatcgcgca gatgctgttt cgtcggaagc ggcttcagga tcat
//
LOCUS       DASWOG010000552         6997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_270595,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000552 DASWOG010000000
VERSION     DASWOG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270595"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGB93_11315"
     CDS             <1..190
                     /locus_tag="VGB93_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146712.1"
                     /translation="LKTLTEIKVGRYPEDVVASADGTTAYVTDWFSDAISIVDLAHGK
                     ETRQIAVAAGPRDLVVVP"
     gene            complement(192..680)
                     /locus_tag="VGB93_11320"
     CDS             complement(192..680)
                     /locus_tag="VGB93_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0146713.1"
                     /translation="MSVLKIMNSRRWLCAAAAISVAGVLPALSATPVPTARGAYLARI
                     TGCIGCHTPRLHDGGVAESRLMSGGDHPIAGNLGRFFPPDVTPDKTTGLGTWTVSDIA
                     KAIKTGRTPDGRILSSAMPWRTQFSAMTDADADAIALYLQSLPPIRNAVPPPLPPLAP
                     KN"
     gene            complement(690..1694)
                     /locus_tag="VGB93_11325"
     CDS             complement(690..1694)
                     /locus_tag="VGB93_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0146714.1"
                     /translation="MRRPCFRDARFWLLLAAFVCFAAALILPSTREQTNGVDVLFTVD
                     ITGSMNTRDYSENGHPISRLDHIKAVLRQTLVDLPCQSHVGVALFAERRIFLLFNPIE
                     VCANFSPITESISAFDWREGWEGDSHIAEGLYRAIALADDLHSDLVFMTDGQEAPPLP
                     WSGGPTFDGRPGAVKGLVVGVGGYGLSPIPRYDSSGREIGFYGEDDVLQESRFGIPPP
                     DAVNRPGYNPRNAPFGDVHVNNNEQLSSVREPYLKSLAQKTGMAYTHLTDAPDFIEAL
                     RTHATPHPVSTTVSRGPIAAALGLICLGCVYIVLPLISWLRRQNWWRRRQHAVPVAAR
                     "
     gene            complement(1691..2302)
                     /locus_tag="VGB93_11330"
     CDS             complement(1691..2302)
                     /locus_tag="VGB93_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaK protein"
                     /protein_id="HEY0146715.1"
                     /translation="MTTFALTHPARLVLLQISALWGRIKTGVLVALVIVLALCTVAAF
                     AMWGRDAARNRTIVALAHNHDIAVAQNAAAKLLFARAYFLATHGQLDEAQALVSLFDL
                     RGSDKARADLHYDLGNGRLLAAFDKIDMADFDAAGALVGLAQGDYIEAMRLNPDDWDA
                     RYNFDVAARLVRQYPSFVNTPDPRRQGPRPIWTELPNTPQGEP"
     gene            complement(2299..3324)
                     /locus_tag="VGB93_11335"
     CDS             complement(2299..3324)
                     /locus_tag="VGB93_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0146716.1"
                     /translation="MVAKPDFLWLLLLAPVPFLVSTLRRAPFPSLDGIPRDPLSSLIS
                     FLIKAAAALAIGAGAIALAQPYRLAENVKLVATGAHVVLVMDRSLSMDFTMDDDEVGS
                     GNESKTQLATRMLMDFVNRSPHDQFGVVAFSSAPIFVLPITGHRDAVKAAITALRRPG
                     IEGTNVGLGLQLALNMIGHDPEATSPVVLFASDGEYGGAGFFDFSLQDALRRAFQREH
                     AHLYWLFLRAHKGNTDVFSPPPSGEEDSPHTKPERHMDLFFKTLGVTYRVFWADSPHA
                     IQDAVAEINRLETRPVTYYEEIPRRDVTPVFYGIAAFASLLLVLAKFAETEFRRSSTF
                     GGRVNAT"
     gene            complement(3330..4289)
                     /locus_tag="VGB93_11340"
     CDS             complement(3330..4289)
                     /locus_tag="VGB93_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nonribosomal peptide synthetase MxaA"
                     /protein_id="HEY0146717.1"
                     /translation="MPMRCRAISSKYERIMRRALTAILIFLGASLAAQAQVQSVKLVT
                     PRLFGHFVGDVLEDEVDVRVDDGVELAPASVPQPGPLNQWLELSSSRVETQSDQGATL
                     YRLYFAYQTFYPALDARQLDVPGFTLSFKSGDHIYPAQVPAWSFGISPLREILPPAKA
                     SGGQYMQPDAPPPYYNLQRESYLAFGLLVATLIALALLAYHLAWWPFGARAHRPFTEA
                     SRTIRKLFAGAEAGSAYRQALVVLHRAVDSTAGRAIFSEDVPAFLAQHPAFARLGEQF
                     RRFFASSQSVFFSDKPAAGIGAFSAAELRQFSDQLAATERSAS"
     gene            complement(4252..5103)
                     /locus_tag="VGB93_11345"
     CDS             complement(4252..5103)
                     /locus_tag="VGB93_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0146718.1"
                     /translation="MTSVAATVSYRPRERAEGIVIGAHRGRNAGGQGVFLDQVTYLRQ
                     PDPRRIDLRLTLRDPFETIYVRRFEQRLAIALYAVVDLSRSMSFKGTARKMTLVGDLC
                     LSLAHSAHELGDAFGIIGCDTEARPDFFIPATRNRGIEPEIARRFEDFVPERRSAVGL
                     LQAATYLAGKRKLVFLVSDFRMPMAEIEKILESLSRHDIVPILVSDSAEETELPSFGL
                     IDVRDLESGASKVVFMRPALRQRWRAEAAARRQTLDGLFLRCGRPAFELKDRFDADAL
                     SRHLLEV"
     gene            complement(5100..6137)
                     /locus_tag="VGB93_11350"
     CDS             complement(5100..6137)
                     /locus_tag="VGB93_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0146719.1"
                     /translation="MTAASIRSADSNLDSWQQQALIFEKEVAKAVIGQERVIRLLTIA
                     VFARGHVLLEGDVGVGKTTLLRAVARAIGGAYERVEGSVDLMPSDLIYYTYLNEEGRP
                     KVDPGPVLRHGEDLSIFFFNEINRARPQVHSLLLRLMAERSANAFNRDYNFPYLQVFA
                     DRNRIEKEETFELPAAARDRFFMEVSVKTPSEPQIRQELIFDPRFHDVDALVATVKEG
                     VIDFRQLPAISAAIQSGVHASPTLQKYVLDLWLAVQTPGSFGVSLPGVDMTRLIRGGG
                     SPRGLSYLVRGARVHAWLHGRDAVVPEDIRSLFQEIIAHRIFLDPVYELRQEGLTRDL
                     CAAIFASVPTP"
     gene            complement(6278..6595)
                     /locus_tag="VGB93_11355"
     CDS             complement(6278..6595)
                     /locus_tag="VGB93_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEY0146720.1"
                     /translation="MKTYRSFNRLVILLGGVAVLSLGASLGALAYDGQHCKEPGVCWE
                     PQPNMPEKLAGSKYDVRGLEEPHEIAKQGNSERAMEARNAKRTAYFHKSGKWVYDVDQ
                     IPE"
     gene            complement(6663..>6997)
                     /locus_tag="VGB93_11360"
     CDS             complement(6663..>6997)
                     /locus_tag="VGB93_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146721.1"
                     /translation="NDDYWTYPKNIHDQGVFETVYGGARAMMGPHNGDLTLDQFMQVI
                     AWVRHLYKDPVNQAFWLTDAQKKNYKPYNDAEGKRIAALPISTPGQCQATAVQEKKEQ
                     DEEDKESQ"
BASE COUNT         1525 a         2114 c         2088 g         1270 t
ORIGIN      
        1 cttgaagacg ctcaccgaga tcaaggtcgg gcgctacccg gaagatgtcg tcgctagcgc
       61 ggatggcacg actgcctatg tcacggactg gttttccgac gcgatctcga tcgtcgatct
      121 cgcgcatggc aaggaaacgc gccagatcgc ggtcgcagct ggcccgcgcg atctcgtcgt
      181 cgtgccttaa gctaattttt gggcgccaac ggcggcagcg gcggcggcac cgcgttgcgg
      241 atcggcggca gggattgcag atagagcgca attgcgtcgg cgtccgcgtc ggtcatggcg
      301 ctaaactggg tgcgccaggg catggcggag gaaagaatgc gtccgtcggg cgttcggccg
      361 gttttgattg ctttcgcgat atcagacact gtccacgtac caagtcccgt cgttttgtcc
      421 ggcgtgacat cgggcggaaa aaagcggccc agattgcccg caatcggatg atcgccgccc
      481 gacatcaggc gcgattcagc aaccccgcca tcatgcaggc gcggcgtatg gcaaccgatg
      541 cagccggtga tccgcgcgag ataggcgccg cgtgccgtcg gcactggcgt tgcgctcaaa
      601 gccggcaaaa cgccggcgac acttatcgca gcggcagcgc aaagccatcg tcgcgagttc
      661 ataatcttca aaacactcat tgaatctgct cacctggccg cgaccggcac cgcatgctgg
      721 cgtcgccgcc accagttctg acgtctgagc caggaaataa gcggcaggac gatataaacg
      781 caaccgaggc agatgagacc caaagccgcc gcgataggcc cgcgcgagac cgtcgtgctc
      841 accggatgcg gagtggcatg cgttcggaga gcctcgataa agtccggcgc atcggtgaga
      901 tgcgtgtagg ccattccagt cttttgcgcg agacttttga gatagggctc gcgcaccgac
      961 gataattgtt cgttattatt gacatgcaca tcgccgaacg gcgcattgcg cggattatat
     1021 ccgggacgat tgacggcgtc aggcggcggg attccgaagc ggctttcctg cagcacatcg
     1081 tcctcgccat agaaaccgat ctcgcggccg ctgctgtcat atctggggat cggcgacagc
     1141 ccgtagccgc cgacgccgac gaccagcccc tttacggccc ccggcctgcc gtcgaacgtc
     1201 gggccgccgc tccagggcaa cggcggcgct tcctgtccgt cggtcataaa gacgagatcc
     1261 gagtgaagat catcggccaa agcgatcgcg cgataaagcc cctcggcaat atggctgtcg
     1321 ccctcccagc cctcacgcca atcaaacgcc gagatggatt ccgtgatcgg cgaaaaattg
     1381 gcgcagacct cgatcggatt gaagagcaga aaaattcgcc tttcggcaaa cagcgccacg
     1441 ccgacatggg actgacatgg cagatcaacg agcgtttgcc gcagcacggc cttgatatga
     1501 tcgagccggc tgatcggatg accgttctcg ctgtaatcgc gcgtgttcat actgccagtg
     1561 atatcgaccg tgaagaggac atcgacgccg ttggtctgct cgcgagtcga cggcaaaatc
     1621 aatgcagccg cgaagcacac aaaagccgcg agcagcagcc aaaagcgcgc gtcgcggaag
     1681 cacggccgtc tcatggctct ccctgcggtg tgttcggcag ttccgtccat atcggacgcg
     1741 gcccttgccg gcgtgggtcc ggtgtattca cgaaacttgg atattgacgc acgagccgcg
     1801 cggcgacgtc aaaattatag cgcgcgtccc aatcgtccgg attgagccgc atcgcctcga
     1861 tgtaatctcc ctgcgccaaa ccgacgagcg cccccgccgc gtcgaaatcg gccatatcga
     1921 tcttgtcgaa tgccgccagc aagcggccat tgcccaagtc gtaatgcaga tccgcccttg
     1981 ctttatcgct gccgcgtaga tcaaagaggc tcaccagggc ctgcgcctca tcaagctgac
     2041 cgtgggtcgc caagaaataa gcgcgggcga atagaagctt cgccgcggcg ttctgcgcga
     2101 cggcaatatc gtgattatgc gccagcgcca cgatggtccg attgcgcgcg gcatccctgc
     2161 cccacatggc aaaagcggcg accgtgcaaa gggcgagaac aatcacaagc gcaacgagta
     2221 cgccggtttt gatgcgcccc cacaatgcgc tgatttgaag gagaacgagg cgggccggat
     2281 gggtgagagc aaaggttgtc atgtcgcgtt cacccgtccg ccgaaagtgg aggaacgacg
     2341 gaattcggtc tccgcgaact tggccaagac caacagcaaa gacgcgaaag ctgcaatgcc
     2401 atagaagacc ggcgtcacgt ccctgcgtgg gatttcctcg taataggtca cgggccgcgt
     2461 ctcgagccgg ttgatttcgg caacggcatc ttgaatggca tgtgggctgt ccgcccagaa
     2521 cacccgatag gtcacgccga gtgtcttaaa gaagagatcc atatgccgct cgggcttcgt
     2581 atgcggcgaa tcttcctcac cactgggcgg cggcgagaat acatccgtgt tgcccttatg
     2641 tgcgcgcagg aagagccaat aaagatgcgc atgttcgcgc tgaaaggccc tacgcaaggc
     2701 gtcttgcaga ctgaaatcga agaacccggc gccgccatat tcgccgtcgg aggcaaaaag
     2761 aacgactggc gatgtcgcct ccgggtcgtg gccgatcata ttgagcgcga gttgcaaacc
     2821 aaggccgacg ttggtgccct cgatacccgg tcgccgcagc gcggtgatcg ccgccttgac
     2881 ggcatcgcga tggccggtga tcggaagaac gaaaatcggc gcgctcgaaa aggcaacgac
     2941 gccgaattgg tcgtgcgggc tgcggttgac gaaatccatc agcatccgcg ttgcgagctg
     3001 ggttttagat tcattgccgg acccaacctc gtcatcatcc atggtgaaat ccatgctgag
     3061 actgcggtcc atgacgagca cgacatgcgc gcctgtcgcg acaagtttca cattttcggc
     3121 gagccggtaa ggttgggcaa gtgcgatcgc gccggcgcca atggcaagcg cagcggcggc
     3181 cttgataagg aaactgatca acgatgatag agggtcgcgc gggataccat caagcgaagg
     3241 aaacggcgcg cgccgcaaag tcgagacgag gaacggcaca ggcgccagca gcagtaacca
     3301 aagaaaatcc ggcttcgcga ccatgtagtt cacgatgccg acctttccgt tgccgcgagc
     3361 tgatcgctga actgccgaag ctctgccgcg ctgaaggcgc cgatgccggc agccggctta
     3421 tcgctgaaga aaacgctctg tgaacttgca aagaaacgcc tgaactgctc ccccagccgc
     3481 gcaaacgccg gatgttgcgc gagaaaggcc gggacatctt cggaaaagat tgcgcggccc
     3541 gccgttgaat ccaccgcgcg atgcaggacg acgagcgcct gccggtaggc cgaacccgcc
     3601 tcagcgccgg caaacaattt gcggatcgtg cgcgatgcct cggtaaacgg ccgatgcgcg
     3661 cgagcaccga acggccacca cgcgagatga taagcgagca gcgccaaggc aatcagcgtt
     3721 gccacgagaa gcccaaaggc gagatagctc tcgcgctgaa gattataata aggcggcggc
     3781 gcatctggct gcatatattg gccgccggag gccttggcag gcggcaaaat ctcacgcaac
     3841 ggcgaaatgc cgaatgacca ggccggcacc tgcgccggat agatgtgatc gccagactta
     3901 aacgacaacg tgaagccggg cacgtccaat tggcgagcgt cgagcgccgg atagaatgtc
     3961 tgataggcga aatagagtcg gtacagcgtc gcgccttgat cgctttgcgt ctcgacacgg
     4021 ctgctcgaga gttcgagcca ttggttaagc ggccctggct gcggcaccga cgccggtgcg
     4081 agttcgactc cgtcatcgac gcggacatcg acctcgtctt cgagcacatc gccaacgaaa
     4141 tgaccgaaca gacgcggcgt cacgagctta accgactgga cctgggcctg cgccgcgagc
     4201 gacgcaccca gaaagatcaa aatggcggtt aaggccctgc gcatgatgcg ctcatacttc
     4261 gaggagatgg cgcgacagcg catcggcatc gaacctatcc ttcaactcga acgccggacg
     4321 cccacaacga agaaagagcc cgtccaatgt ctgccgccgt gccgccgctt ccgcacgcca
     4381 gcgctggcgc aaagccgggc gcatgaaaac aaccttgctg gcaccgctct caagatcgcg
     4441 cacatcgatc aaaccgaacg acggcaattc ggtctcttct gcggaatcgg agacaagaat
     4501 cggcacgatg tcgtggcgcg aaagcgattc cagaattttc tcgatctccg ccatcggcat
     4561 acggaaatcc gaaacgagga acacaagctt gcgcttaccg gcaaggtagg tcgccgcctg
     4621 caggaggcct acggcactcc ggcgctccgg cacaaaatct tcgaaccgcc gggcgatttc
     4681 gggctcgata cccctgttgc gcgtcgccgg aatgaagaaa tccgggcgcg cctccgtatc
     4741 gcagccaatg atgccgaacg cgtcacccaa ttcatgcgcc gagtgcgcca gcgaaaggca
     4801 aaggtcgcca acgagcgtca tcttccgcgc cgtgcccttg aagctcatcg atcgcgacag
     4861 atcgacgacg gcataaagag cgattgccag cctctgctca aaacggcgga cgtagatagt
     4921 ctcgaacggg tcgcgcaacg tcagccgcag atcgatccgg cgcgggtcag gttggcgcag
     4981 ataggtaacc tgatcaagga agacgccttg cccgccggca tttcggccac gatgcgcccc
     5041 aatgacaatc ccttcggcac gctcgcgcgg ccgataggaa accgtcgcag ctacactggt
     5101 catggcgtcg gcaccgacgc gaaaatcgcg gcgcaaagat cgcgcgtcag cccttcctgt
     5161 cgcaactcat agaccggatc gaggaagatg cgatgcgcga tgatttcctg aaagagcgag
     5221 cggatatcct ccggcacgac agcgtcgcgg ccgtgcagcc atgcatggac gcgggcaccg
     5281 cgaaccaagt agctcaagcc gcgcgggctg ccgccaccac ggataagccg cgtcatgtct
     5341 acgccgggca atgaaacacc gaaagagccc ggcgtctgta ccgccaacca gaggtcgagt
     5401 acgtattttt ggagcgtcgg gctggcgtgc acaccactct gaatggcagc ggagatcgcc
     5461 ggcaattgac gaaaatctat gacgccttct ttcaccgttg cgacaagcgc atcgacgtca
     5521 tggaagcgcg gatcgaaaat gagctcctga cggatttgcg gttcgctcgg cgtcttgacg
     5581 ctgacctcca tgaagaagcg atcgcgcgct gccgcaggca gctcgaacgt ctcttccttt
     5641 tcgatccgat tgcgatcggc gaagacctgg agatagggaa agttataatc gcggttgaaa
     5701 gcgttggcgc tgcgttcggc catgagacgc aagagcagcg aatggacctg cggccgggcg
     5761 cgattgatct cgttgaaaaa gaagatcgac agatcctcac cgtgccgcag cacagggccg
     5821 ggatcgactt ttgggcgtcc ctcttcgttc aggtaggtat aatagatgag gtcgctcggc
     5881 atgagatcga ccgaaccttc gacgcgctca taggcgccgc cgatcgcgcg cgccacagca
     5941 cgcaagagcg tcgtctttcc gacgccgaca tcgccctcaa gcaggacatg gccgcgcgcg
     6001 aaaaccgcga tggtcaagag ccggatgacc cgctcctggc cgataaccgc ttttgccact
     6061 tccttctcga agatcagcgc ctgctgctgc caggaatcaa gattcgaatc ggcactgcgg
     6121 atcgaagctg ccgtcatgag aatcatcact atttgaattt gccgcgaaca atgcggagag
     6181 gcgtttattg gcccgcgcgc ggcgcaagtc ttagcttgcg ccgcggcgaa gtacgacaat
     6241 gcgtgcataa gatctcagtt cgtcaatatt tcgacgatta ctctgggatc tgatcgacgt
     6301 cgtagaccca tttgccgctc ttgtggaaat aggcagtgcg cttggcgttg cgagcttcca
     6361 tggcgcgctc ggaattgccc tgtttggcaa tttcgtgcgg ctcttcgagg ccccttacgt
     6421 cgtacttcga gccggcgagc ttctccggca tattcggctg cggctcccag catacgccgg
     6481 gttctttgca atgctgaccg tcataggcaa gcgcgccgag gctggcgccg agcgacaaaa
     6541 cggcgacgcc acccaaaaga atcacaaggc gattaaacga ccgatatgtt ttcatgcgtt
     6601 ttcctccact ttcctcgctt tcacgcgagt ataaaccgat tgataccgcg tgccggaacg
     6661 gctcactgcg attctttgtc ttcttcgtct tgttctttct tttcctgcac cgccgtcgcc
     6721 tggcactggc cgggcgtcga aatcggaaga gccgcgatac gtttgccttc cgcgtcgtta
     6781 tagggcttgt aattcttttt ctgcgcgtcg gtgagccaga aggcctggtt gaccggatcc
     6841 ttatagagat gccgcaccca ggcgatcacc tgcatgaact ggtcgagcgt caggtccccg
     6901 ttgtggggac ccatcatcgc gcgggcaccg ccgtaaaccg tctcaaacac gccctggtca
     6961 tgaatgttct ttggataggt ccagtagtca tcgttca
//
LOCUS       DASWOG010000553         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_142075,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000553 DASWOG010000000
VERSION     DASWOG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142075"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..942
                     /gene="scpB"
                     /locus_tag="VGB93_11365"
     CDS             208..942
                     /gene="scpB"
                     /locus_tag="VGB93_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY0146722.1"
                     /translation="MSADEPMMGEALRVVEAMLFAAAEPLEESEIARRMPDGLAAADV
                     LARLKQDYSNRGVNLVCVGKKWLFRTASDLGWLLALDSIEPKKLSKAALETLAIIAYH
                     QPVTRADIEDIRGVAISKGTLDVLLETGWVRLRGRRRTPGRPLTYGTNETFLLHFGLE
                     QIGDLPGLDELKGAGMFDGALPQGFAVPQPRDDTALMEDEDPLDGDAQEEFVELSAEA
                     AADGEPGESETDAAPEDDDENSLAEE"
     gene            1497..2250
                     /locus_tag="VGB93_11370"
                     /pseudo
     CDS             1497..2250
                     /locus_tag="VGB93_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          422 a          653 c          701 g          474 t
ORIGIN      
        1 ggtgaaggcc tcgtctctcg ccgcggcgct tgaaatggtt cgtcagggtg ccatcagcct
       61 gcgtcaggat caggccttcg cgccgctgtg gatcaagaag cgcgatgcgg ccgcgagccc
      121 cgcgccggta caataaggga gctgaacttg gccgacgtcg cacgactttt tgccgagatt
      181 ctcgacgaag aggccgagag tgaatccatg tcggccgacg aacctatgat gggcgaggcg
      241 ctgcgggtcg tggaagctat gctgtttgcg gcggcggagc cgcttgagga atccgaaatc
      301 gcccggcgga tgccggatgg tttggccgct gccgatgtac ttgcgcggct gaagcaggat
      361 tattccaatc gcggcgtcaa tcttgtgtgc gtcggcaaga aatggctgtt tcgcaccgct
      421 tccgacctcg gctggctttt ggcgctggat tcgatcgagc cgaagaaact ctccaaggcc
      481 gcgctcgaaa cgctcgccat catcgcctat caccagccgg tcacgcgtgc ggatatcgaa
      541 gacatccgtg gcgtcgcgat ctcgaagggc acgctcgacg tgctgctcga aacgggatgg
      601 gtgcgtttgc gcggccgccg ccgcacgccg ggacgtccgc tgacctacgg caccaatgaa
      661 accttcctgc tgcatttcgg ccttgaacag atcggcgatt tgccggggct tgacgaattg
      721 aagggggcgg gcatgttcga cggcgcgctg ccgcagggct ttgccgtgcc gcagccgcgc
      781 gacgacaccg cgctgatgga agacgaagac ccgctcgacg gcgatgcgca ggaagagttc
      841 gtcgaattga gcgcggaagc tgcggcggac ggcgaaccgg gagagtctga aacggatgca
      901 gcgcccgagg acgatgacga gaactcgctt gcggaggaat agtcgctgcg ccgcagtcgc
      961 atacgcgaca tcttcgtccg caggctcccg ttgcctctgc acatagtttg agccgttggt
     1021 gcgcccggcg catgcatcga gcgcgaacat catgagatga cgcacgcaaa ctgaagtcgc
     1081 gatcatcaca gaagattcat gccaacggcg tacgcatctt attactaggg tccagcaaag
     1141 cttgcgtacc gatgccgcgt tagagacttg cacgtccaat tggcggcctt gattgatttg
     1201 cgtcaatgcc ggtcgaagtg cgatttgcca tacttgaggg gcctagaaac acgcacatac
     1261 tttcgtccca tttgtcgggc tattcgtcgc agcatcggga aggcgtatcg gcgtggcgtt
     1321 gacagagccg ccgctgctcg gaattcgtta aggttctatc cttatgcccg tcggccctcg
     1381 gcgctagttg ccaggtctgg aattccggcg ctcaccaaag tcagtagcgc tcgcatttgg
     1441 agccgcgtgt cgcgcggtct tcggtatttc gccgatttcg cttcgaaagt tgttagatga
     1501 attttttcaa gtttgtcacg gaccgccgcg ctgctttcaa tgtttcagag ctgacgattc
     1561 gacgaccttc tattgcgttt tttggagctt tcgtcgcgct cgcgcttggg atcatcggct
     1621 tccgtcaatt gccgatcacg ttgctgccaa atgtcgatcc gccgctcgtt tctgtcttcg
     1681 tcactgactt cggcgcgacg ccgaccgagc ttgaaacaca agtgacggag aaggtcgagg
     1741 ccgccgtttc aagcattcct ggcgtccatc atgtgatgtc ctccatcggc gacggcattt
     1801 ctgcgacgac gatcagcttt gacttgtcgg tcaatacgga tcacgtgacc gaggcggtga
     1861 agcgggccgt cgcgcagatt gtacccgaat tgccgcgcgg ggcggatgcg ccggttgtca
     1921 ggcgcgtcac cgcggttggg ctaaatatcg ttacctatgc agcgtcgtca tcgaaactga
     1981 ccccggcaca attgtaagcc ttcgtcgacg caaagatcat caatggcctc aaggcactgt
     2041 ccggtgtcgg cagggtcgag cgtatcggcg gcgcgacgcg cgaaatttcg gtccggctcg
     2101 atcccgttgc acttgccgcg catggtctca cggcgtccga cgtcgatgcg caggtccaga
     2161 tcgcgcgaaa gaatgctggt gcaagcgaaa gcctgagcga tctcgccgca acgccgatcc
     2221 gccagcccaa aggcggtaca attcggctcg
//
LOCUS       DASWOG010000554         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_159714,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000554 DASWOG010000000
VERSION     DASWOG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159714"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..3639
                     /gene="gltB"
                     /locus_tag="VGB93_11375"
                     /pseudo
     CDS             1..3639
                     /gene="gltB"
                     /locus_tag="VGB93_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173423.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
     gene            3704..>4542
                     /locus_tag="VGB93_11380"
     CDS             3704..>4542
                     /locus_tag="VGB93_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY0146723.1"
                     /translation="MGKVTGFLEIDRRDRRYAPASDRIRNYKEFMIPLSEEATKDQAA
                     RCMNCGIPYCHNGCPVNNQIPDWNDLVYNSDWEEAARNLHSTNNFPEFTGRVCPAPCE
                     AACTLNLTDQPVTIKTIECTIVDRAFAKGWVRPEPSERRTGKKIGVVGAGPAGLACAQ
                     QLARVGHDVHVFDKHLKMGGLLRYGIPDFKMEKHLIERRVQQMEAEGVNFHGGVNVGV
                     DLDPANLLDDCDALVLAGGAEKPRDLPIPGRDLAGIHFAMDFLPQQNRRVSREPFHTN
                     QPIL"
BASE COUNT          887 a         1336 c         1442 g          877 t
ORIGIN      
        1 cgcgctttct atgtatatca tgcggtgctg atggagccct aggacgaccc cgcggccgtg
       61 gccttcgccg atggccggca gatcggtgcg acgctcgatc gcaatggttt gcgtccggcg
      121 cgctatgtcg tcaccgatga cggctttgtc gtcatggcgt cggaagtcgg cgtgctgccg
      181 atcccggaag agaagatcgt caccaaatgg cggcttcagc cgggccggat gctgctcgtc
      241 gatctcgaac agggccgcat catttccgat gatgagatca aggcggagat gtcgaagctg
      301 gcgccttatg gtgagtggct gtcacgcacg cagatcaagc ttgaggaatt gccggcgccg
      361 tccggacagc gcccgccgcg cggcgatgcc tccttgctcg atcgccagca ggcattcggc
      421 tacacgcagg aagacgttgc gctgctgatg gagcccatgg ccgagacagg ccaggaggcg
      481 atcggctcga tgggcaccga cacgccgatc tcggcgctgt cgtcgaagtc gaagctgctc
      541 tacacttatt tcaagcagaa ttttgcgcag gtgacgaacc cgccgatcga tccgatccgc
      601 gaagacatcg tcatgagcct cgtgtccttc atcgggccgc ggccaaacat tttcgaccgc
      661 gaaggcaatt ccaagcgcaa gcgccttgag gtcaagcagc cgattctgac caacgacgat
      721 ttggaaaaga tccgcaacat cggcatggtc gaggatacgt tcgacacgcg cacgctcgac
      781 gtgacctatg gggcagacaa gggcgccgaa ggcatggccg aggcgctcga tcggctctgc
      841 agtcgtgccg aacaggcgat cacgagcggc tatacgatca tcgttctgtc tgaccgtctg
      901 gttggaccgg atcggattcc gattccgtcg ctgctcgcca ccgcggccgt acatcatcat
      961 ctgattcgca agggtctgcg cacgtctgtc ggccttgtcg tcgagaccgg cgaagcgcgc
     1021 gaagtgcacc actttgcttt gctcgccggc tatggcgccg aggcgatcaa cccctatctc
     1081 gcgtttgaaa cgctgtacgc aattgcctcc gaattgccca aggatgtcga tgggcatgag
     1141 gcggtgaagc gctatatcaa gtccatcgac aagggcctga tgaaggtcat gtcgaagatg
     1201 ggcatttcga cttatcaatc ttactgcggc gcgcagatct tcgatgcgat cggcctcgcc
     1261 gatagtttcg tagcccgcta ttttaccggc acggcgagcc gcatcggcgg catcggcctt
     1321 accgaagtcg cacaggaatc ggcgcgccgg catcaggatg ctttcggcaa tgcgccgatc
     1381 tatcgcaacg cgctcgatgt cggcggcgaa tacgcctatc gcattcgtgg cgaagtacat
     1441 tcctggacac cgcagacggt ctcgctgctt cagcacgcgg tgcgcagcaa ttcggccgag
     1501 acctataagt cctatgcagc gctgctgaat gaggagagcg aacggccgct gacgatccgc
     1561 agtctgttcc gtatccgccc ggccgaggaa gacggccgtg tgccggtgcc gttagaagaa
     1621 gtcgagtcgg tagcaacgat cgtccggcgc tttgcgacgg gcgccatgtc gttcggctcg
     1681 atctcgcgtg aggcgcatac gactctcgcc gtcgcgatga accgcatcgg cggcaaatcg
     1741 aatacgggcg agggcggtga ggaatccgat cgcttcaagc cgttgcctaa cggcgattcg
     1801 atgcgctcgg cgatcaagca ggtcgcttcc ggccgcttcg gcgcgacggc ggaatatctg
     1861 gtcaacgccg acatgatcca gatcaagatg gcgcaaggtg cgaagcctgg cgagggtggg
     1921 caattgcccg gccacaaggt cgatgccatc atcgccaagg tgcgccactc gacgcagggc
     1981 gttggtctca tctcgccgcc gccgcaccat gacatttatt cgatcgagga tctgaagcaa
     2041 ctcatctacg atctgaaaaa cgtcaatccg cgcgctgacg tgtcggtgaa gctcgtttcg
     2101 gaaatcggtg tcggcacggt tgccgccggt gtgtccaagg cgaaggctga tcacgtcacg
     2161 atctcgggct atgagggcgg tacgggcgct tcgccgctga cctcgatcaa gcacgcgggt
     2221 tcgccgtggg agatcggcct tgccgagacg catcagacgc tggtgctcaa tcggctgcgc
     2281 tcgcgcattg cggtgcaggt cgacggcggt ttgcgcactg gacgcgatgt cgtggtcggc
     2341 gcgctgcttg gcgccgatga gttcggtttt gccaccgcgc cgctgatcgc ggccggctgc
     2401 atcatgatgc gcaagtgtca tctcaatacc tgtccggtcg gtgtcgcgac gcaggacccg
     2461 gtgctgcgca agcgcttcgt tggccagccc gagcatatca tcaattattt cttcttcgtg
     2521 gctgaggaag tgcgcgaatg gatggccaag ctcggctatc gcacattcga tgaaatggtc
     2581 ggccagatgc agatgctcga ccgcgccaag gtcgtcgagc attggaaggc acacggcctc
     2641 gatttctcga aattgttcca taagccgact gccgacgggc cggaggcgat ctatcattcc
     2701 gaagggcagg atcatcagct cgagaaagtg ctcgaccgcg gcctgatcgc ggaggcgcag
     2761 ccggcgatcc agcgcggcgc gaaggtgcgg atcgaaacga cgatccgcaa cagcgatcgc
     2821 acggcaggcg ccatgctttc gggggagatc gcccgcgtct acggccacac cggcctgccg
     2881 gacgaaacgg tccatatcaa aatgaagggc acggctggac agagcttcgg tgggtggctc
     2941 gccaagggcg tgacgatcga actcgaaggt caggccaacg actatgtcgg caagggcctt
     3001 tcaggcggca agatcatcat ctatccgccg gcagaatcgc gcgtcgtgcc ggaagagtca
     3061 atcatcgccg gcaatacggt gctttacgga gcgatcgccg gcgagtgcta cttgcgcggc
     3121 gttgctggcg agcgttttgg cgtgcgcaat tcgggcgcga tcacggtcgt tgaaggcacc
     3181 ggcgatcacg gctgcgaata tatgactggc ggcattgtcg tcgtgatcgg caaaacgggg
     3241 cgtaatttcg ccgccggcat gtcgggcggc atcgcgtatg tccttgatga ggatggcgat
     3301 ttcgcccagc gctgcaatct ggcgatggtc gaactcgaac cggtcgtcgc ggaagaagag
     3361 aagatgcagg agagctatca ccagggtggc gatctcgact tccacggccg cgtcgatgtc
     3421 atgcgcgaca tgacccggtt cgatgccgag cgcctgcatc agctgatcag caatcacgcg
     3481 gcctacacgg gctcgatgcg ggcgcgtctc atccttgaca attgggatga gtacaaaggc
     3541 aagttccgga aggtgatgcc ggtggaatat cggcgcgcgc tggccgaact cctggcgcag
     3601 gaagaggatg cgccgccatt cgccgctgcc ggcgaataaa cgtcagggca ttcgcagtta
     3661 atcgatttcg cccgccggta acgcgggcgc gtttggggct aatatgggca aagttacggg
     3721 cttcctcgaa atcgaccggc gagaccggcg ctatgcgccg gcgtccgacc gcatccgcaa
     3781 ttacaaggag ttcatgatcc cgctttccga ggaagcgaca aaggatcagg ccgcgcgctg
     3841 tatgaattgc ggcatcccgt actgtcacaa cggctgcccg gttaacaatc agatcccgga
     3901 ttggaacgat ctcgtctata attccgattg ggaagaggcc gcgcgcaatc ttcattcaac
     3961 aaataatttt ccagaattta ccggccgcgt ctgcccggcg ccttgcgagg cggcctgcac
     4021 gctcaatctc accgatcagc cggtaacgat caaaacgatc gaatgcacga tcgtcgatcg
     4081 cgctttcgcc aagggttggg tcagaccgga gccgtcagag cgccgcacgg gcaagaaaat
     4141 cggcgtcgtc ggcgccggtc ccgcgggcct tgcctgcgcg cagcagctcg cgcgcgttgg
     4201 ccatgatgtg catgtcttcg acaagcatct gaaaatgggc gggctgctgc gttatggcat
     4261 tcccgatttc aagatggaga agcatctgat cgagcgccgc gttcagcaga tggaagcgga
     4321 gggggtgaat tttcacggcg gcgtcaatgt cggcgtcgat ctcgatcccg ccaatcttct
     4381 cgatgattgc gatgcgctcg tgctcgcggg cggggcggag aagccgcgcg atctgccgat
     4441 tcccggccgc gatctcgcgg gcattcactt cgcgatggat tttctgccgc agcagaaccg
     4501 ccgcgttagc cgcgagccgt tccataccaa tcagccgatc ct
//
LOCUS       DASWOG010000555         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_159737,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000555 DASWOG010000000
VERSION     DASWOG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159737"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..798)
                     /locus_tag="VGB93_11385"
     CDS             complement(283..798)
                     /locus_tag="VGB93_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0146724.1"
                     /translation="MHPNSLGFDVSVVQRIPVVLTALDVVCDTTGMGFAVVARVTEDR
                     WVACQVRDRINFGIEPGDELVIETTICRDIRQSGRPVAIDEVATDRIYSQHPTPKLYG
                     FQSYISVPLLLPDGSFFGTLCAVDTKPARVNTPGAVGMFKLFAELIGFHLADRYRDMA
                     ASRSCDNAGAG"
     gene            complement(996..1072)
                     /locus_tag="VGB93_11390"
     tRNA            complement(996..1072)
                     /locus_tag="VGB93_11390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Arg,seq:tct)
     gene            complement(1105..1410)
                     /locus_tag="VGB93_11395"
     CDS             complement(1105..1410)
                     /locus_tag="VGB93_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEY0146725.1"
                     /translation="MTARIYRPSKTATQSGRAKSKRWVLEFPPEFAPKIDSLMGWTGT
                     QDMRRQVRLWFGTQEEAVAYAEKNGIVFLLEEPKPTTRKIQSYSDNFKPTRLGQWTH"
     gene            complement(1437..1513)
                     /locus_tag="VGB93_11400"
     tRNA            complement(1437..1513)
                     /locus_tag="VGB93_11400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1477..1479),aa:Pro,seq:tgg)
     gene            complement(1585..>1835)
                     /locus_tag="VGB93_11405"
     CDS             complement(1585..>1835)
                     /locus_tag="VGB93_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY0146726.1"
                     /translation="LFDFKHEQSVMMWMKNTFIPLDMLFMDKTGKVVGIIADAEPMSE
                     KILTVSKPTDAVLELRGGTAARIDLKVGDQVRDPIFAP"
BASE COUNT          408 a          525 c          514 g          388 t
ORIGIN      
        1 tttgtcgttt cgagattctc gaacggctta agacagacat gagatttgag ataatcctcg
       61 aggctcgtgt cggtcgcgtt gccagccagc actttgcgct ggcgcgcgcg gattccaaag
      121 tgccgataaa tgaattctcg gacagcaatg gagaaggcat ttttgcgccg catggccgcc
      181 aatcgaaacg agcgcgttgt catcattcaa taggaagtac gcgaaattgg caacatccaa
      241 agtcttgaaa gatcacaaac tcattgattt gacatgatct tttcaacccg ccccagcgtt
      301 atcgcaagat cgggatgctg ccatatcgcg gtaacgatca gcgagatgaa agccgatcaa
      361 ttcggcgaaa agcttgaaca tgccgacggc gcctggcgtg ttcacacggg caggctttgt
      421 atcgacggcg cacagggtgc cgaagaagct gccgtcaggc agcaacaacg gcactgaaat
      481 ataactctgg aagccataaa gtttcggcgt cggatgctgc gaataaatgc gatcggtcgc
      541 aacctcgtcg atggcaaccg gccgcccact ctgccgtata tcgcggcaaa tggtggtctc
      601 gatcacgagt tcgtcgcccg gctcaattcc gaaatttatc ctgtcgcgga cctggcaggc
      661 gacccagcgg tcttcggtca cacgcgcaac gacagcaaag cccatgccgg tcgtatcgca
      721 gacgacatca agggccgtga gcacgaccgg aatgcgctga acgacgctga catcgaatcc
      781 gagcgaattg gggtgcatcg cgaactcccc cgagcccaaa cctatgcgac ttttggacca
      841 tatcaatcag cggcatcttc gaaaaggcta ttttgtcttg agcggcttgg ctaaagctag
      901 acaggaaagc acgtttgcgg attatccgcg gaaagttagt gccgaagatc attttgaaaa
      961 cggcaatttg aacccggtga atagtttgcg gaatatggcg accccggcag ggctcgaacc
     1021 tgcgacctgc cgcttagaag gcggctgctc tatccagctg agctacgggg tcctgcccgg
     1081 cgccattcgc tcggccgcgc cggtttagtg cgtccactgg cctaagcgtg tcggcttaaa
     1141 attatcggaa taggattgga tcttccgcgt cgtcggcttc ggctcctcaa gcaggaagac
     1201 gatgccgttc ttctcggcgt aggcgaccgc ctcctcctgc gtgccgaacc agagccggac
     1261 ttgccggcgc atatcctgtg ttcccgtcca gcccatcaga gagtcgatct tcggggcgaa
     1321 ttccggcgga aattccagaa cccagcgttt gcttttcgcc cggcccgatt gcgtcgcagt
     1381 tttgctgggg cggtaaatcc gtgcggtcat ctcgaacatc cgatcttcga aattaatggt
     1441 cggggcagca ggattcgaac ctgcgacctg aagtacccaa aacttccgcg ctaccaggct
     1501 gcgctatacc ccgaagagcg ataagccgcc ctgtccgctc cgtgttcagc taccatgcgg
     1561 ctggaaggcc gacaaggcgg gggctcaagg cgcgaaaatc gggtcgcgca cctggtcgcc
     1621 caccttgaga tcgatgcgcg ccgccgtgcc gccccgaagt tcgagcacgg cgtcggtcgg
     1681 cttgctgacc gtcaaaatct tttccgacat cggctccgca tcggcgataa tgccgacgac
     1741 ctttccggtc ttgtccatga agagcatgtc gagcggaatg aaggtgtttt tcatccacat
     1801 catgacgctt tgctcatgct tgaagtcgaa cagca
//
LOCUS       DASWOG010000556         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_32085,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000556 DASWOG010000000
VERSION     DASWOG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32085"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VGB93_11410"
     CDS             complement(<1..988)
                     /locus_tag="VGB93_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY0146727.1"
                     /translation="MTDTAIPHVERAAVNLSEDWISAILGTSIFVLALLGLTGADLLG
                     WAATAKIWLVPDTALAPIGKSYAALGGLPALVLTYVVFLAVLSAAAALLRANVGRFAL
                     AFSAVFWLASIAWIIGSYAHFAAATPVDIAKTGVTWSLHLTPEGGLILALIAGLIVGN
                     FFPRFADWLSEAIRPEFYVKIAIVILGGVIAITVAGKLNLASTVFLRSLAAIVEAYLI
                     YWPVVYYISRKYFGFSREAAVPLASGISICGVAAAIATGSAIRARPQVAVLVSSLVVV
                     FAVVELLILPFLANTFLAHEPLVAASWLGLAVKTDGAAVAAGGIAESLISAKN"
     gene            complement(1084..1305)
                     /locus_tag="VGB93_11415"
     CDS             complement(1084..1305)
                     /locus_tag="VGB93_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146728.1"
                     /translation="MTASADILATRLRARGRETSAGIDERLARNREFSENLTADFVVD
                     NSGDATTAAAQLAAILQNLAAAQPIAAAL"
     gene            complement(1369..1665)
                     /locus_tag="VGB93_11420"
     CDS             complement(1369..1665)
                     /locus_tag="VGB93_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146729.1"
                     /translation="MSQGAGMFVCLVGPSGAGKDTLLRLVKIKLAAQPGFIFPCRRVT
                     RAASPHEDHLVLTETAYLEGVRAGRFALAWRAHGLGYAIDHDVLPALASGHVVT"
     gene            1852..>1991
                     /locus_tag="VGB93_11425"
     CDS             1852..>1991
                     /locus_tag="VGB93_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0146730.1"
                     /translation="MDEPHADKPQRRELIAFRIGAQEFCVNIMSVREIRGFTPATALP
                     RAP"
BASE COUNT          449 a          595 c          620 g          327 t
ORIGIN      
        1 cattcttcgc gctgatcagc gactcggcga taccgccggc agcgaccgcc gcaccgtccg
       61 tcttcaccgc aagaccaagc cagcttgccg cgacgagcgg ctcatgcgcg aggaatgtat
      121 tggcgaggaa cggcaggatc agaagttcga ccaccgcgaa gacgacgacg agcgaagaga
      181 cgagaaccgc cacttgcggc cgggcgcgga tggcgctgcc ggtggcgatt gccgcggcga
      241 cgccgcagat cgaaatgccg gaggcaagtg gcacggcggc ttcgcgggag aagccgaaat
      301 atttccgtga aatgtaataa accaccggcc aatagatcag ataggcctcg acgatggcgg
      361 cgagcgagcg caggaagacc gtcgaagcca gattgagctt gcccgcaacg gtgatggcga
      421 tgacgccgcc caggatcacg atggcgatct tgacataaaa ttccggccgg atcgcctcgc
      481 tgagccaatc ggcaaatcgc ggaaagaaat tgccgacgat aaggccggcg atcagggcga
      541 ggatcaagcc gccctcgggc gtcagatgca gagaccaggt gacgccggtc ttggcgatgt
      601 cgacgggggt cgctgccgcg aaatgcgcgt agctgccgat gatccaggca atcgaggcaa
      661 gccagaagac cgccgaaaag gcgagtgcga agcggccgac gttggcgcgc agcagcgcgg
      721 ccgcggcgct gagcaccgcg agaaacacga cataggtcaa aacgagtgcc ggcagcccgc
      781 cgagcgccgc ataggatttg ccgatcggcg ccagcgccgt atctggcaca agccagattt
      841 tggcggtcgc ggcccagccg agcaaatcgg cgccggttaa tccaagcaac gcgaggacaa
      901 aaatcgatgt gccgaggatg gcggaaatcc agtcctcgct cagatttacc gccgctcttt
      961 caacgtgcgg aatcgccgta tctgtcatga aatccagctc cccttatcaa tttattatag
     1021 tacactattt tcatagacta tcgctgtaaa tacatacttt gtcagtaaat taggttgccc
     1081 ctctcaaaga gcggccgcga ttggctgggc ggcagccagg ttttgcaaaa tggccgcaag
     1141 ctgcgcggcg gctgtcgtcg catccccgga attatcgacg acgaaatcgg cggtgagatt
     1201 ttcggaaaat tcgcgattgc gggcgagccg ctcgtcgatg ccggctgagg tttcgcgtcc
     1261 gcgcgcacga agccgcgtgg cgagaatgtc tgccgacgcc gtcacgagca ccgtcgcgat
     1321 gcacggaaaa tgagccttcg cagcggtgat cgcgtcgcgc gagacatttc aggtaacaac
     1381 gtgaccggag gcgagcgccg gcagaacatc gtggtcgatc gcgtagccaa gaccatgcgc
     1441 gcgccaggcg agcgcgaaac gccccgcgcg aaccccttcg agataggcgg tctcagtcag
     1501 aacgagatgg tcttcatgag gcgaagcggc acgggtgacg cggcggcacg ggaaaataaa
     1561 gccgggttgt gcagcaagct tgattttgac gagtcgcagc aacgtgtcct tgccggcgcc
     1621 gcttgggccg acgagacaga cgaacatgcc ggcgccttgc gacaagagga aaatgcctca
     1681 tgaaacccga aagatttttg cgtcaaaacg gcccgatacg ccagcgctgt ctcaagtccg
     1741 ccgccatcgt cagtcccggc ccactacgac tttattcact tttttcgagc acattctacc
     1801 gagccattca cccccgccgt gggcacgcgg cattttcatt caggcgagtt catggacgag
     1861 ccgcacgcag acaaacccca acggcgcgaa ctgatcgcct tccgcatcgg cgcgcaggaa
     1921 ttttgcgtga atatcatgtc ggtgcgcgaa atccgcggct ttacgcccgc gaccgcgctg
     1981 ccgcgtgcgc c
//
LOCUS       DASWOG010000557         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_361210,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000557 DASWOG010000000
VERSION     DASWOG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361210"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGB93_11430"
     CDS             <1..662
                     /locus_tag="VGB93_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146731.1"
                     /translation="VALLTPRDVPNKEASPLRELAALKRPQVWLTLAIGAIGFGGMFA
                     VYTYLASTLADVTHVSSLYLPLVLCAFGLGLTAGKLIIPLFADRALMPTAGALLVWTA
                     ATLALYAFAAHNIWLVTFDVFLIGCGGALGTVLQARLMDVSGEAQALPAALNHSAFNT
                     ANAIGPWLGGIAIGAGFGWTSTGWVGFALALGGLVIWLLSVVIDSRLEGDPKLIEDPA
                     E"
     gene            complement(729..>1994)
                     /locus_tag="VGB93_11435"
     CDS             complement(729..>1994)
                     /locus_tag="VGB93_11435"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0146732.1"
                     /translation="SANVEKNGKIIERRLADDCTYTQLDGAPLSVSGRSVTLIRNVGH
                     HMITDAVLDADGAQIPETLLDAAVTGLIASHDLHAKGRRNSAKGSVYIVKPKMHGPDE
                     VALADRLFGAVEDFLGLKRYTLKMGIMDEERRTSANLKAVIKAATNRVFFINTGFLDR
                     TGDEIHTSMEAGPMIRKEDMKKTKWIAAYEDSNVDVGLRCGLPGRAQIGKGMWAMPDS
                     MSAMLVDKIPHLTSGANTAWVPSPTAAILHATHYHRVDVRKRQKELRARPPAKLADLL
                     TIPISRGNFAPDAVQEELDNNCQGILGYVVRWVDQGIGCSKVLDIHDVGRMEDRATLR
                     ISSQHIANWLQHGIVNETQVSDTLKRMAKIVDRQNHDDGAYQPMAPAFDGPAFKAAED
                     LIFKGRVQPNGYTEWILHDRRREAKEQAA"
BASE COUNT          326 a          635 c          601 g          432 t
ORIGIN      
        1 ttgttgccct gctcacgccg cgcgacgtcc ccaacaaaga ggcgagtcca ctgcgcgagc
       61 ttgcggcgct caagcggccg caggtctggc tgacactcgc gatcggcgcc ataggcttcg
      121 gcggcatgtt cgccgtttac acctatctcg cctccacgct ggccgacgtg acgcatgtgt
      181 cgagcctcta tctgccgctc gtgctctgcg ccttcggcct cggcctcacg gctggcaagc
      241 tcatcatccc gcttttcgcc gatcgcgcgc tgatgccgac ggcgggcgcg ctcctcgtct
      301 ggacggcggc aacgctcgcc ctttacgcgt tcgctgcgca taacatctgg ctggtgacgt
      361 tcgatgtctt cctcatcggc tgcggcggtg cgcttggcac ggtcttgcag gcgcggctga
      421 tggatgtgtc cggcgaggcg caagccctgc cggcagcgct caaccattcc gccttcaaca
      481 cggcgaacgc catcggcccc tggcttggcg gcatcgcgat cggcgcagga tttggctgga
      541 catcgaccgg ctgggtcggc tttgccctag cgctcggcgg ccttgtcatc tggctgcttt
      601 ctgtcgtgat cgacagccgt ctagagggcg atccgaaact tatcgaagac ccggctgagt
      661 aaaactcgcg cgtctatcat cgcatcggat ttatccggat accgcttcgt acttttcggc
      721 ccgatgctct aagccgcctg ctccttcgcc tcacggcggc gatcatgcag aatccattcg
      781 gtgtagccgt tcggctgcac gcgccccttg aagatcaaat cttctgccgc cttgaaggcc
      841 ggtccgtcga aggccggcgc catcggctga taggcaccat cgtcatggtt ttggcgatcg
      901 acgatcttcg ccatccgctt cagtgtgtct gacacttgcg tctcgttgac gatgccgtgc
      961 tgcagccaat tggcgatgtg ctggctcgaa atgcgcaacg tggcgcgatc ttccatgcgc
     1021 ccgacgtcgt ggatatcgag caccttcgag cagccgatcc cctgatcgac ccagcgaacg
     1081 acatagccca gaatgccctg gcaattgttg tcgagttctt cctgcaccgc atccggtgcg
     1141 aaattgccgc gcgaaattgg gatcgtcagc agatcggcca gtttcgccgg cgggcgggcg
     1201 cgcaattcct tctgccgctt gcggacatcg acccgatgat aatgcgtcgc gtggaggatc
     1261 gccgcggtcg gtgatggcac ccaggccgtg ttggcgccag aggtcagatg cggaatttta
     1321 tcgacgagca tggcggacat gctgtcgggc atcgcccaca tacccttgcc gatctgcgcc
     1381 cggcccggca ggccgcaacg caggccgaca tcaacattcg aatcctcata ggcggcgatc
     1441 cacttcgtct tcttcatatc ttccttgcgg atcatcggcc cggcttccat cgacgtgtga
     1501 atttcgtcgc cggtgcgatc gaggaaaccc gtattgatga agaagacgcg atttgtggcg
     1561 gccttgatga cggccttcag attggcgctg gtgcgccgct cttcgtccat gatgcccatc
     1621 ttgagcgtgt aacgcttgag gccgagaaaa tcctcgaccg cgccgaagag acgatcggca
     1681 agcgcgactt cgtccggacc gtgcattttc ggcttgacga tatagacaga gcccttggcg
     1741 ctgttgcgcc ggcccttcgc gtggagatcg tgactcgcga tcaggcctgt caccgccgcg
     1801 tcgagcaacg tctcgggaat ttgcgcgcca tcggcatcga gcacggcatc ggtaatcatg
     1861 tgatgcccga cattgcggat gagcgtcacg ctgcggccgg aaaccgagag cggcgcgccg
     1921 tcgagttgcg tgtaggtaca atcgtcggca aggcggcgct cgatgatttt gccgttcttt
     1981 tccacattgg cgga
//
LOCUS       DASWOG010000558         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_159816,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000558 DASWOG010000000
VERSION     DASWOG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159816"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            580..>1715
                     /locus_tag="VGB93_11440"
     CDS             580..>1715
                     /locus_tag="VGB93_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEY0146733.1"
                     /translation="MKFLFEDESFSFETLRTAGFANYAGADLGEVLVTARAIPEGDET
                     AWLMQWEATATRVARIGESSLAAGHRISAREALFRASNYYRSAEFFKRANPADDVDVA
                     RLSSRSTQTFIQAAKLLDTPFETVEIAYEGGMLPGYLFLVDNSGEPRPTVIYNNGFDS
                     IQEESYFAIGAAALRRGYNVLAFDGPGQGAAIRKSKLTFRHDWEAVVTPVIDFALTRR
                     EVAPDKIALFGYSLGGYLVARAAAFDRRAAAIILDDGVLDFYAAFQHILPPFLLSWID
                     ERKDDAANSVLRMLMDLKTGIRWAIQNGMWTFGASSPSAFVRASKLYTVADVAADIRA
                     ATLVLGGDNDQFLKGQPQKIYAALKVPKTLVTFTEAEGAGEHCQ"
BASE COUNT          328 a          520 c          507 g          360 t
ORIGIN      
        1 gtctacttca aaggggtatg ctcgctgggt tggtgtgtgc gaatgatttg aagggagacc
       61 caacgcttgt gtgggccggc cgggcttatg gcattcgcac ggcgtcgcag tcgatcgcga
      121 agagggggtt gcgacccata acgagatcca tcgtggcgat cgaggctctc ttcccatcgc
      181 ggatggatcg agatggtcat ccttgactcg gccacaatcc agcgaaaagc cgaggccttt
      241 tttgattggc gtcaacgact gttgtccgcg ccttcgacta aaagtctgcg ttagcgggtg
      301 atgccccttc ctccgcgagc acgtcgaagg ccgtcggcaa ccgctccgcg tttggccgca
      361 atggcaaacc ttcccgatga tctgcgcttt ggtggccagc tcgaactcgg ccgacaccgt
      421 catgaccacg gtctctatgc tggcggcaac ttgttgtttc acgcgtcgac gcctactggc
      481 gccagttggt tcggcaagat gcgatcgttc gtgcggagct cgcaaaaccg gagcagggag
      541 tcagctccta aaccctctta aatccaggag acctcccaaa tgaaattctt gtttgaagac
      601 gagtcctttt ccttcgaaac cttgcgcacc gcaggcttcg caaattacgc cggtgccgac
      661 ctcggcgaag tccttgtgac ggcgcgagcg atccctgaag gcgacgagac ggcttggctg
      721 atgcaatggg aggcgacggc cactcgcgtc gctcggattg gcgaaagctc gcttgccgct
      781 ggacatcgta tcagcgcgcg cgaggcttta tttcgcgcct cgaactatta ccgctcggcc
      841 gaatttttca aacgcgccaa tccggcggac gacgtagatg tcgcccggtt gtccagccgc
      901 tcgacgcaaa cgttcatcca agcggcaaag ctcctcgaca cccctttcga gaccgtcgag
      961 attgcctatg aaggcggaat gttgccgggc tacctgtttc ttgtcgacaa ttccggcgag
     1021 ccgcggccaa ccgtcattta caataacggc ttcgactcga tccaagagga atcctatttt
     1081 gccatcggcg ccgcggcctt gcgccgtggt tacaatgtgc ttgccttcga cggtccgggc
     1141 cagggcgccg cgatccggaa atcgaaactc acattccgcc acgactggga agccgtcgtc
     1201 actccggtga tcgactttgc gttaacccgc agggaggtgg cgccggacaa gattgcgctg
     1261 ttcggctaca gcctgggtgg atatctggtg gctcgcgccg cggccttcga tcggagggcc
     1321 gccgccatca ttctcgacga cggcgttctc gatttctacg ccgcattcca gcacattctg
     1381 ccgccattcc tgctatcgtg gattgacgag cgcaaggacg atgcagccaa ctcggtgctg
     1441 cggatgctca tggacctcaa gacgggcatt cgctgggcaa tacagaacgg aatgtggacc
     1501 ttcggcgctt cctcaccgag cgcgtttgtt cgcgcgagta agctctacac ggtggccgac
     1561 gtcgccgccg acatccgcgc cgcgacattg gtgcttggcg gcgacaacga ccagttcctc
     1621 aagggccagc cgcaaaagat ttacgcggcg ctgaaggtgc caaagactct cgtaaccttc
     1681 accgaggcag agggcgctgg cgagcactgc cagat
//
LOCUS       DASWOG010000559         5227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_86382,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000559 DASWOG010000000
VERSION     DASWOG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86382"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..293
                     /locus_tag="VGB93_11445"
     CDS             30..293
                     /locus_tag="VGB93_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146734.1"
                     /translation="MKTTGKLALAVSAIFWVSAAVAQDDSTTTTTHTDHYDNSHHDGA
                     YVGVPGVVGVHVGGDSHSGCETRSKTVQDNETGDSRTHTETNC"
     gene            complement(336..1862)
                     /locus_tag="VGB93_11450"
     CDS             complement(336..1862)
                     /locus_tag="VGB93_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEY0146735.1"
                     /translation="MKMVSIRTLQDLLAVGRGDAVAVTAPGTQALTYDAFRKLVADTV
                     ATLNGSGIGRNDRVAIVLPNGPEMATSFVAIATGATAAPLNPAYKGDEFEFYMSDLGA
                     KALVVEAGSKSPALEVAQKLGIAVLTLTPDPSVGAGIFTLSGGGEKKAAANSGYGETD
                     DVALILHTSGTTSRPKIVPLTQGNVTASAQNIAAALEFCDADRGLNIMPLFHIHGLIA
                     GLLAPLSPGSQIFCMPGFNALKFFGWMEESKPTWYTAVPTMHQAILARANNNKETIAR
                     YPLRFLRSSSASMPPQLIGELEATFHAPLIEAYGMTEASHQMASNPLRGTRKPGAVGL
                     PAGPEIGIMDEDGNLLGIGEIGEVVIRGDNVTAGYENNPSANASAFTNGWFRTGDQGM
                     IDADGHLSLTGRLKEIINRAGEKISPREVDEALLDHPAVEQVVTFAMPHDKLGEEVAA
                     AVVLREGQTVTEKELRDFLSNRLAAFKTPRKIIFLAEIPKGATGKLQRIGLAQKLGLA
                     "
     gene            complement(1882..2883)
                     /locus_tag="VGB93_11455"
     CDS             complement(1882..2883)
                     /locus_tag="VGB93_11455"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0146736.1"
                     /translation="MRICVIGAGAIGGLLAVKLADAGEEVSVIARGPHLAAINANGLK
                     LIEGDKETVAKVKASDRIADVGTQDLVILGMKAHQVAAVVRDLPALYEPETTVLTAQN
                     GIPWWYFFKHGGAHEGARLESVDPGGIIADNLPIERVLASVVYPAAEIERPGIIKHIE
                     GYRFSLAEVDGKPTERLETIAAAFTRAGFKAPIVADIRTELWTKLWGNATFNPISALT
                     HATLEDICRFGLTRKLAEDVMREVKAIGEALGIRFRIPLEKRIAGAEAVGAHKTSMLQ
                     DVETGRALEADALLGSVIELGRIVDVASPHLDALYAVVKLLGKTLADHKGRLVVEPA"
     gene            3136..4425
                     /gene="oxlT"
                     /locus_tag="VGB93_11460"
     CDS             3136..4425
                     /gene="oxlT"
                     /locus_tag="VGB93_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY0146737.1"
                     /translation="MSVAAGEEVIVSENSSANRWVQLLIGIVCMTMIANLQYGWTLFV
                     GPLHDAFGWKLASIQVAFTIFVVCETWLVPFEGYFEDLFGPRPLVIFGGVLVGIAWVL
                     NSQITGLSGLYIAQAIGGIGAGAIYGSCVGNAVKWFPDHRGLAAGLTAAGFGIGSALT
                     IVPISHVIAAHGYQNAFLYFGIIQGIVVCICALLLRSPNKDDAYQAAIAAGEHGRQFA
                     PKEMLRTPLFWLLYAMFVMMAAGGLMATAQLAPIAKDFGVAKVPVSFFAFTMPALVFA
                     LALDRTLNGLTRPFFGWVSDRIGRESTMFIAFGVEGLAILALSKYGHTPGLFVLLSGL
                     VFFGWGEIYSLFPATCTDSFGSKFATTNAGLLYTAKGTAALLVPFSSKLTEWTGSWHL
                     VFLIAAGLNIVTALLAIFVLRPLRRKYAEANGQSLRA"
     gene            complement(4407..4829)
                     /locus_tag="VGB93_11465"
     CDS             complement(4407..4829)
                     /locus_tag="VGB93_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146738.1"
                     /translation="MTAAAFLALVLNAHQPAIQPVHEIYRPPLASFDLLVDNDSVRVL
                     NARNVGVSLRNSEYTDLDISHSFHVACALPMNYGLWRVRAWADTVYNLLPVTIVEYKY
                     YDPTYVELEIEPSTFTDAIDGNGTGPHRVHIDFYARND"
     gene            complement(4961..>5227)
                     /locus_tag="VGB93_11470"
     CDS             complement(4961..>5227)
                     /locus_tag="VGB93_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146739.1"
                     /translation="PATIVLSFIIGFVLSSAFAAIIVYAQELLPERIGAVAGLFFGLA
                     FGIAGLAAAVLGVVADATSITFVYHVTAFLPLIGVLAYFLPRNL"
BASE COUNT          986 a         1556 c         1603 g         1082 t
ORIGIN      
        1 ccgcggtctt tcaatgaggg agaggacaca tgaagacgac tggcaagctc gcgctcgcgg
       61 tgagcgcaat cttttgggtt tccgcggctg ttgcgcagga cgactcgacg acaacgacaa
      121 cccatacgga tcattacgac aactcgcatc acgacggtgc ctatgtcggc gtgccgggtg
      181 ttgtcggcgt gcacgtcggt ggcgattcgc actcaggttg cgagacgcgc agcaagacgg
      241 tgcaggacaa tgagacgggt gactcgcgca cccataccga aacaaactgc tagatcggca
      301 acgggacgcc tggtggcgtc ccgtttcgtt ttgctctagg cgagaccaag cttttgggcg
      361 aggcctatgc gctgcaattt gcctgttgca cccttcggga tttccgcaag gaagatgatc
      421 ttgcgtggtg tcttgaacgc cgcgaggcgg ttggaaagaa agtcgcgcag ctctttctcg
      481 gtcacagtct gaccttcgcg caggacgaca gcggcggcca cttcctcccc gagcttgtcg
      541 tgcggcatcg cgaacgtcac gacttgttcc accgcaggat gatcaagcag cgcctcatcg
      601 acctcgcgcg gcgaaatctt ttcgccggcg cgattgatga tttccttgag ccgtccggtc
      661 aggctcaaat gtccgtcggc atcgatcatg ccctgatcgc cggtacggaa ccagccattg
      721 gtgaaggcgc tcgcatttgc ggaaggattg ttctcataac ctgcggtgac gttgtcgccg
      781 cggatgacga cttcgccaat ctcgccgatg ccgagcagat tgccgtcttc gtccatgatg
      841 ccgatctcag ggccggcggg caggccgacg gcgccaggct tgcgcgtccc acgcagcgga
      901 ttggacgcca tctgatgcga cgcttcggtc atgccgtaag cctcgatcag cggcgcgtgg
      961 aaggtagcct caagttcgcc aatcaattgc ggcggcatcg acgctgacga ggagcgcagg
     1021 aagcgcagcg gataacgcgc gatcgtctct ttgttgttgt tcgcgcgcgc gagaatcgcc
     1081 tgatgcattg tcggcacagc cgtgtaccag gtcggtttgc tctcttccat ccagccaaag
     1141 aacttcagcg cgttgaaacc gggcatgcag aaaatctggc tgccgggcga gagcggcgca
     1201 agaaggccgg cgatcagccc gtggatgtgg aacagcggca tgatgttcaa gccacgatcg
     1261 gcgtcgcaaa attcgagcgc cgcggcgatg ttctgcgcag atgcagtgac attgccctgc
     1321 gtgagcggga cgatcttcgg ccgggaggtg gtgcccgacg tgtgaaggat cagcgcgaca
     1381 tcgtcggttt cgccatagcc ggaattggcg gcagctttct tttcgccgcc gcccgacagc
     1441 gtgaaaatac ccgcgccgac gctcgggtcc ggcgtcaaag tcaaaactgc gatgccaagc
     1501 ttctgtgcga cctcaagtgc cggcgacttg ctgccagcct cgacgacaag cgccttggcg
     1561 ccgaggtcgc tcatgtagaa ttcgaactcg tcacccttgt aggccgggtt caacggcgcc
     1621 gcggtcgcgc ccgtcgcaat ggcaacgaag ctcgtcgcca tctccgggcc atttggcagg
     1681 acaatggcca cacggtcatt acggccgatg ccagatccgt tgagcgtcgc gaccgtgtcg
     1741 gcgacgagtt tgcggaacgc atcataagtc agggcctgcg tgcccggcgc cgtaacggcg
     1801 acagcatcgc cgcgtccgac ggcgagcaga tcttgcaggg tccggatcga aaccatcttc
     1861 attccccaat aaagcgccgg atcaggccgg ctcgacaaca aggcggcctt tgtgatcggc
     1921 cagggttttt ccaagaagct tgacgacggc gtagagcgcg tcgaggtgtg gcgacgcaac
     1981 gtcgacaatg cgaccgagtt cgattaccga gccgagcagg gcgtcggctt cgagcgcgcg
     2041 gccggtttcg acatcctgca gcattgacgt cttgtgcgcc ccgacggcct cggcgccggc
     2101 aatgcgtttt tcgagcggga tacggaaacg aatcccgagc gcctcgccga tcgctttcac
     2161 ctcacgcatc acgtcttctg cgagctttcg cgtcaggccg aaccggcaaa tgtcctcaag
     2221 cgtcgcatgg gtcagtgcgc tgatcgggtt gaacgtcgca ttgccccaaa gcttcgtcca
     2281 aagctcggtg cggatatcgg cgacgatcgg cgccttgaat ccggccctgg tgaaggccgc
     2341 ggcgatggtt tccagcctct cagtcggctt gccatcgact tcggcgagcg agaagcgata
     2401 gccttcgatg tgcttgatga ttccggggcg ctcgatctct gctgcaggat agacgacgct
     2461 agcgagcacg cgctcgatcg gcagattgtc ggcaatgatt ccacccgggt cgacgctttc
     2521 gagccgcgcg ccttcatgcg cgccgccatg tttgaagaaa taccaccacg ggatgccgtt
     2581 ctgcgccgtg agcactgtgg tttccggttc gtagagcgcc ggaagatcgc gcacgacggc
     2641 ggcgacctga tgcgccttca tgcccagaat aaccagatcc tgcgtgccga catcggcaat
     2701 gcgatccgag gccttgacct tcgcgacggt ttccttgtcg ccttcaatca gtttcaaacc
     2761 gttggcgttg atcgccgcaa gatgcggccc gcgagcaatg acgctgactt cctcgcctgc
     2821 gtctgcgagt ttcaccgcga gcagtccgcc aatcgcgcca gcaccaatta cgcatattct
     2881 catcaatcga aacccttcca gcgatggtcg cggggatcgg cggctgcgcc gggacatatc
     2941 tggcgtgtca atgccgcatc tcagatggct ttgtccaccc ttgcgagtgc gtgaaaatgc
     3001 gcacttagcg aatatgctgc atcgcaaaac attgcaaacg attggctttt ggcgctcaac
     3061 cgcagaaaaa attgttgcgt ggcgacgttt ctgtcggcac attcccgcgc cattacaaaa
     3121 aggggcgcgt agaaaatgag cgttgctgcg ggggaagaag tcatcgtttc tgaaaacagc
     3181 agcgccaatc gttgggtgca attgttgatc ggcatcgtct gcatgacgat gatcgccaat
     3241 ttgcagtatg gctggacact ctttgtcggc ccgctccacg acgcgttcgg ttggaagttg
     3301 gcctcgatcc aggtcgcttt caccattttc gtggtctgtg aaacctggct cgtgcccttc
     3361 gaaggctatt tcgaagatct gtttggcccg cgcccgctcg tcattttcgg cggcgtgctt
     3421 gtcggcatcg cgtgggttct gaactcacag atcaccggtc tctccggcct ttacatcgcc
     3481 caggcgattg gcggcatcgg cgccggcgcg atctatggct cctgcgtcgg caacgcggtg
     3541 aaatggtttc ccgaccatcg cggccttgcg gccggcctca ccgccgcggg tttcggcatc
     3601 ggatcggctt tgaccatcgt gccgatctcg catgtcatcg cagcgcacgg ctatcaaaac
     3661 gcgtttctct atttcggcat catccagggc atcgtcgtct gtatttgtgc gctgctgctg
     3721 cgttcgccga acaaggacga tgcatatcag gccgcgattg ctgcaggcga gcacggacgt
     3781 cagttcgcgc ccaaggaaat gctacgcacg ccgttgttct ggctgctcta tgcgatgttt
     3841 gtaatgatgg ccgccggcgg cctgatggcg accgcgcagc ttgccccgat cgccaaggat
     3901 tttggcgttg ccaaggtgcc cgtgagcttc ttcgccttca ccatgcccgc attggtcttc
     3961 gcgctggcgc tcgaccgcac gctcaatggt ttgacacgac cgttcttcgg ttgggtgtcg
     4021 gacaggatcg gtcgtgaaag cacgatgttc atcgccttcg gcgtcgaggg tctggcgatc
     4081 ctcgcgctga gcaaatacgg ccacacaccg ggcttgttcg tgctgctcag cggcctcgtc
     4141 ttcttcggct ggggtgaaat ctattcgctg tttccggcaa cgtgtacgga ttccttcggc
     4201 tcgaaattcg cgacgacgaa tgcgggcctg ctttatacgg ccaagggcac cgctgcgctg
     4261 ctcgtgccgt tttcaagcaa gctcaccgaa tggaccggca gctggcatct cgtcttcttg
     4321 attgcggccg ggctcaatat cgtgaccgcg ctgctggcga tcttcgtgct gcggccgctg
     4381 cggcgtaaat acgccgaagc gaacggtcag tcgttgcggg cgtagaaatc gatatgcacc
     4441 cggtgcgggc cggtgccgtt gccgtcgatc gcgtcggtga aagttgacgg ctcgatctcg
     4501 agttcgacat aggtcggatc gtaatattta tattcgacga tcgtcaccgg tagcagatta
     4561 tagacggtgt ccgcccaagc gcgcacgcgc cataggccgt aattcattgg cagcgcgcag
     4621 gcgacgtgga aagagtgcga aatatctaga tcggtatatt ccgaattgcg taacgacacg
     4681 ccgacattgc gtgcattgag gacgcgcacg ctgtcattat caacgagcag atcgaagctc
     4741 gccagcggcg gccggtagat ttcatgcacc ggctggatcg ccggctgatg cgcatttagg
     4801 acgagagcca ggaacgccgc cgccgtcata atcttctccg agtcgcgccg cgaaacccgg
     4861 cacatggtcg actttttgcg ccgtgccgcg cgaaaagtct atcggcgcgg ggatcacgac
     4921 atcgcggggc aggccaatcc tccggaggat tggcctgcgc ttacagattc cgcggtagga
     4981 aataggcgag gacgccgatt agcggcagga aggccgtcac atgatagaca aaggtgatgc
     5041 tcgtcgcatc tgcgaccacg ccgagcaccg ccgccgcgag acctgcgatg ccaaatgcga
     5101 gcccgaagaa gagtccggcg accgcgccga tgcgttcggg caagagttct tgcgcataga
     5161 cgatgatcgc cgcgaaagcc gaggacagaa cgaagccgat gatgaagctc agcacgatcg
     5221 tcgccgg
//
LOCUS       DASWOG010000560         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_325792,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000560 DASWOG010000000
VERSION     DASWOG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325792"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGB93_11475"
     CDS             <1..204
                     /locus_tag="VGB93_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821625.1"
                     /note="responsible for the amidation of carboxylic groups
                     at position A and C of cobyrinic acid or hydrogenobrynic
                     acid; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HEY0146740.1"
                     /translation="CLLGDSVLGVAGTRLRGHEFHYATITEPGTDAPLAEVTDAYGSP
                     AKAGGSRRGHVSGSFFHMIAPVP"
     gene            complement(205..1254)
                     /gene="cobD"
                     /locus_tag="VGB93_11480"
     CDS             complement(205..1254)
                     /gene="cobD"
                     /locus_tag="VGB93_11480"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEY0146741.1"
                     /translation="MIARPSPRPAEIEDEHVAPAVYHGGDLGAARSLFPEAPTPWLDL
                     STGINAIPYPTEAFSQQALTRLPDRHALMRLLTAAAAAYGAKDMQNVVAAPGTQALIQ
                     WLPRLVPGRRVAILGFGYQEYPALWRAAGAEVFVTDDVTDLTNPGVDVAIVVNPNNPG
                     GRLVDPQTLTEIATRLARRGGLLIVDEAFMDLMRPAMSLIPQLPQHGALVLRSFGKAY
                     GLAGIRLGFAVTGLDLAERLRTALGPWAVSGPAIEIGAAALTDQDWLEESAARLAQQA
                     IRLDALLTAAGFSILGGTPLCRLAQHEDAALWFQKFGRRGILTRSFPERPDWLRFGFP
                     GVETDWARLAEALKS"
     gene            complement(1251..2009)
                     /gene="cobF"
                     /locus_tag="VGB93_11485"
     CDS             complement(1251..2009)
                     /gene="cobF"
                     /locus_tag="VGB93_11485"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HEY0146742.1"
                     /translation="MRKILIIGMGAGNPDYMTVQAIDALNKVDVFFIPDKGTEKAALR
                     KLRTDICARFIKTKAHRFVEMPVPTRAGDFSDYKANVADWHAEIEANYTRLLREELGE
                     TKCAGFLVWGDPAIYDSTLRIIEKIQASGGIEFDYQVIPGISAIQALAARHRVPLNRI
                     GESIHITTGRKLAGGYPADADSVVVMLDGEQTFQKLAGEFEIYWGAYVGTDEEVLVSG
                     RLPDVASEIAAIRAERRERNGWIMDSYLIRRPEK"
BASE COUNT          393 a          608 c          630 g          411 t
ORIGIN      
        1 tgtctgcttg gcgatagtgt tttaggtgtg gctgggacgc gcctgcgcgg ccatgaattt
       61 cactatgcga cgattactga gccgggcacc gatgcgccgc ttgccgaagt caccgacgct
      121 tacggttcgc ccgccaaggc aggcggcagc cgtcgtggtc atgtttcggg aagctttttt
      181 cacatgatcg cgccggtgcc ttgatcagga cttgagcgcc tccgccaacc gcgcccagtc
      241 cgtttcgacg ccggggaagc cgaagcgcag ccaatcgggg cgttcgggaa acgaccgtgt
      301 caggattccg cggcggccga atttttgaaa ccatagcgcc gcatcttcgt gttgcgccag
      361 gcgacataaa ggcgtgccac ccaggatcga aaagccggct gccgttaaaa gcgcgtcgag
      421 gcgtatggcc tgctgcgcaa gccgtgccgc agattcttcg agccagtctt ggtccgtaag
      481 ggcggccgcg ccgatctcga tggctggtcc tgagaccgcc cagggaccga gcgccgtgcg
      541 gaggcgctcg gcaagatcaa gcccggttac tgcgaagcct agccgaattc ccgcgaggcc
      601 ataggctttg ccgaacgagc gaagcactaa ggcgccgtgt tgaggtagct gcggaatgag
      661 gctcatcgcc gggcgcatca ggtccatgaa ggcttcatcg acgatcaaca gaccgccgcg
      721 ccgtgcgagg cgtgttgcga tctctgtgag tgtttgagga tcaacgagac ggccacctgg
      781 attgttgggg ttgacgacga tggcgacatc gacgccggga ttcgtcagat cagttacatc
      841 gtcggtgaca aagacctccg cgccagccgc gcgccagagc gccggatatt cctgatagcc
      901 aaatcccaga atcgcgacgc gccggcccgg aactagcctc ggcagccatt ggatcaaagc
      961 ctgtgttccc ggcgcggcca cgacgttctg catgtctttt gcgccgtagg cggccgcggc
     1021 ggctgtcagc agtcgcatca acgcatgacg atccggcaag cgcgtcaaag cttgttgcga
     1081 aaaagcttca gtcgggtagg ggatggcatt gatgccggtc gaaagatcta gccaaggcgt
     1141 tggcgcctcc gggaacagcg aacgcgccgc gccgagatcg cctccgtgat agacggcggg
     1201 ggcaacatgc tcgtcttcaa tctctgccgg tcgcggcgat ggcctcgcga tcacttctcg
     1261 ggccggcgga tcagatagct gtccatgatc cagccattgc gttcgcgccg ttcggcacgg
     1321 atcgcggcga tctcgctcgc gacatccgga agccgacccg aaacgagaac ttcttcatcg
     1381 gttccgacat aggcacccca ataaatttca aattcgccgg caagcttttg gaacgtctgc
     1441 tcgccatcga gcatcaccac cacactgtcg gcgtccgccg gatatccgcc ggcaagcttg
     1501 cggccggtgg tgatgtggat cgactcgcca atgcggttca gcgggacgcg atggcgtgcg
     1561 gccaaagcct ggatggcgct gatgccgggg atgacctgat aatcgaattc aatccctccg
     1621 cttgcttgga ttttctcgat gatccgcaac gtgctgtcgt agatcgccgg atcgccccag
     1681 acgagaaagc cagcgcattt ggtctcgcca agttcctcgc gaagcaatct cgtgtaattg
     1741 gcttcaatct cggcgtgcca gtcggcaaca ttggctttgt aatcggaaaa gtccccggcg
     1801 cgtgtcggca ccggcatttc gacgaaacga tgcgccttcg ttttgatgaa gcgggcacag
     1861 atgtcggtgc gcaattttcg caacgcggct ttctcggtcc ccttgtcggg gatgaagaat
     1921 acatcgacct tgttcagcgc atcgatggcc tggacggtca tatagtcggg attgccggca
     1981 cccatgccga tgatgaggat tttccgcatc gcgccagtct agcttatcaa cactgatctt
     2041 ga
//
LOCUS       DASWOG010000561         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_197772,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000561 DASWOG010000000
VERSION     DASWOG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197772"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VGB93_11490"
     CDS             <1..326
                     /locus_tag="VGB93_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146743.1"
                     /translation="LLKIHFKVPPAHEPWIGAFMGAATGVVTGATGINVIPAVPYLQS
                     LDLERDDLIQALGLSFTVSTMAMAAGRYTVACSLTHHGCWVHHYLPSSPASSACSLVS
                     IRANG"
     gene            complement(438..>2721)
                     /locus_tag="VGB93_11495"
     CDS             complement(438..>2721)
                     /locus_tag="VGB93_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY0146744.1"
                     /translation="YHSDAELESRVAHLTNLQFGITTLKDAATRTPYFCSGCPHNVST
                     KVPEGMRAYAGIGCHYMVQNMDRATEGYTHMGGEGANWVGEALFSTRSHIIQNLGDGT
                     YNHSGVLAIRWAVAARTNITYKILFNDAVAMTGGQALEGDLTVPKLVRQVAAEGAKKI
                     VVITDAPAKYRDIADWPQDVEVYPREDLEPVQRELAEVPGTTILIYDQTCAAEKRRRR
                     KRKEFPEPDKRVFINDIVCEGCGDCGVTSNCVSVQPLETEFGRKRVIDQSSCNKDFSC
                     LNGFCPAMVTVEGAKPKVPGTKPAANDFPPLPHPEIPQIGDRPFAILIAGVGGTGVVT
                     IGAFLAMAAHLEGKFCGSTDMAGLAQKGGAVHSHIKIAAKPDQIHAISVGTGGADLVI
                     GCDLVVSGSAKVLATVRSGETGVVINNAETYPGDFTRNPDFTLPVSAIRQAIEKACAG
                     HASFCDATSRAIALVGTSVAANIFLVGFAWQKGLLPLSEDALMRAIEIVGESVEMNKR
                     AFLWGRREAAEPARVASLLAPKIAPTSSRAMSESFDELFARRQDFLTAYQNRAYADIY
                     VARVKRIIDAEKRVAPGETRLATAVAKSLFKLMSVKDEFEVARLYSDGTFHDNIAAAF
                     DGDIKIKFHLAPPIFARKRGPMPKWTFGPWMMRLFKILAPLKVLRGTPLDLFGLSSER
                     RMERHLRAAYEATLDEIVAKLTSENYDCAVALAALPEKIRGFGHVKFRAVELAKAEEA
                     ELLARFRSDVSLQPMKLAAE"
BASE COUNT          508 a          845 c          828 g          540 t
ORIGIN      
        1 gcctgctcaa aatccatttc aaagtgccac cggcgcatga accctggatc ggtgcgttca
       61 tgggcgccgc caccggcgtc gtcaccggcg cgacaggcat caatgtcatc cccgcagtgc
      121 cctatttgca atcgctcgac ctcgaacgcg acgatctcat tcaggcactt gggttgtcgt
      181 tcacggtttc gacgatggcg atggccgcgg gccgctacac cgtggcgtgt tcactcactc
      241 accatggctg ctgggtgcat cactatttgc cctcgtcgcc ggcctcatcg gcatgttcat
      301 tggtcagtat ccgcgcgaac ggatgagcct cgaaaccttc cgccgcgtct tccagattgg
      361 cttgctcgcg ctcggcacat atctcgcgat cgagggcttt gcgtatatgt agcgcgaaca
      421 cgcgataaac gtagcgccta ttccgccgcg agcttcatcg gctggaggct gacatccgag
      481 cgaaagcgcg ccagcagctc cgcctcctcg gcctttgcca gttcgacggc gcggaacttg
      541 acgtggccga agccgcggat tttttccggc aatgccgcaa gcgcgacggc gcaatcgtaa
      601 ttttcggacg tgagtttcgc gacgatctcg tccagcgttg cctcataagc ggcgcgcaag
      661 tgccgctcca tccgccgctc gctcgacaag ccgaaaagat caagcggcgt gccgcgcaaa
      721 actttgagcg gcgccaatat cttgaagagc cgcatcatcc acggcccgaa cgtccacttc
      781 ggcatcgggc cgcgtttgcg cgcgaagatc ggcggcgcga gatggaattt gattttgata
      841 tcgccgtcga aagccgccgc gatattgtcg tggaacgtgc cgtcgctgta gaggcgcgcg
      901 acctcgaatt cgtccttcac cgacatcagc ttgaagagac tctttgccac cgccgtcgcg
      961 aggcgcgtct cgccaggcgc aacgcgcttc tcggcatcga tgatgcgctt caccctcgcg
     1021 acatagatgt cggcgtaggc gcgattctga taggcggtaa ggaaatcctg gcggcgtgcg
     1081 aaaagctcgt cgaacgattc cgacatcgcg cgcgacgaag taggggcgat ctttggcgcc
     1141 agcaaacttg cgacgcgcgc cggttccgcg gcctcgcgcc ggccccagag gaaagcccgc
     1201 ttgttcattt cgacggactc gccgacgatt tcgatggcac gcatcagcgc gtcttccgac
     1261 aagggcagaa gccccttctg ccaagcgaaa ccgacgagga agatattggc tgcgaccgac
     1321 gtgccaacga gcgcgatcgc gcggctcgtc gcatcgcaga aagaggcgtg gccagcgcag
     1381 gccttctcga ttgcctgccg gatcgcgctg acgggcagcg tgaaatcggg gttgcgggtg
     1441 aaatcgccgg gataggtttc ggcgttgttg atgacgacgc cggtttcgcc cgacctgacc
     1501 gttgcgagga ccttggcgct gcctgagaca acgaggtcgc aaccgatgac gagatcggcg
     1561 cccccggtgc cgacactgat cgcgtgaatc tgatccggct tcgcagcgat cttgatgtgg
     1621 ctgtgaaccg cgccgccctt ttgcgcaaga cccgccatat cggtcgagcc gcaaaacttg
     1681 ccttcaagat gtgccgccat cgccagaaag gcgccaatgg tgacgacgcc cgtgccgccg
     1741 acaccggcga tcaaaatagc aaaaggcctg tcaccgatct gcggaatttc cggatgcggc
     1801 agcggcggaa aatcgttggc cgcaggcttc gtacccggca ctttcggctt ggcgccttcg
     1861 accgtcacca tcgcggggca gaagccgttg aggcacgaaa agtccttgtt gcagctcgac
     1921 tgatcgatca cgcgcttgcg gccgaattcc gtttcgagcg gctgcaccga cacgcaattc
     1981 gaggtgacgc cgcaatcgcc gcagccttcg cagacgatat cgttgatgaa gacgcgtttg
     2041 tcgggctcgg gaaattcctt gcgcttgcgg cgccggcgtt tttccgcggc gcaggtctga
     2101 tcgtaaatga ggattgtcgt gcccggcacc tcagcgagtt cgcgctggac gggttcgaga
     2161 tcttcgcgcg gatagacttc gacgtcttgc ggccaatcgg caatatcgcg atatttcgct
     2221 ggcgcatcgg tgatgacgac gatcttcttg gcgccctccg ccgcgacctg acgcacgagc
     2281 ttgggcaccg tcagatcgcc ttcgagcgcc tggccaccgg tcatagccac ggcatcgttg
     2341 aaaagaattt tgtaagtgat gttggtccgc gcagcgacgg cccagcggat cgccagcacg
     2401 ccggaatgat tgtacgtccc gtcgccgaga ttctggatga tatgcgagcg cgtcgagaac
     2461 agcgcttcgc cgacccagtt cgcgccctcg ccacccatgt gagtgtagcc ttcggtcgcg
     2521 cggtccatat tctgaaccat gtaatggcag ccgatgccgg cataggcgcg catgccctca
     2581 ggcactttcg tcgagacgtt atgcgggcag ccggagcaga aatagggcgt ccgcgtcgcc
     2641 gcgtctttca gtgtcgtgat tccaaactgc agattcgtga gatgggcgac acggctttcg
     2701 agctcagcgt cggaatgata g
//
LOCUS       DASWOG010000562         6916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_234350,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000562 DASWOG010000000
VERSION     DASWOG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234350"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VGB93_11500"
     CDS             complement(<1..490)
                     /locus_tag="VGB93_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146745.1"
                     /translation="MQGASVLFSEMTPPPGQEQRFHNWYDEEHIPLRMDVPGFVSAQR
                     YRDLSENAKGFLAVYEMTDPNVMKTPAYQEVKTKPSETTREMLGTISGFTRYIAAETS
                     VKRQPNDSGVSALDAPLLFAVWFEVPESALADFDAWYELDHVPLLLGCKDWSMVRRFR
                     VVD"
     gene            complement(534..1745)
                     /locus_tag="VGB93_11505"
                     /pseudo
     CDS             complement(534..1745)
                     /locus_tag="VGB93_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            complement(1742..2938)
                     /locus_tag="VGB93_11510"
     CDS             complement(1742..2938)
                     /locus_tag="VGB93_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0146746.1"
                     /translation="MKTAIVNIGRIGSGDWKSPFEPGDAILCEDGLIQSVGTLSAQDV
                     SSADVVIDADGATAIPGLIDSHVHITFGDYTPRQKTVGYLESYLHGGVTTAISASEVH
                     VPGRPKDPVGVKALAVAAARCFKTYRPGGMRVHAGSVILEPGLNESDFAELRNHGVWL
                     AKAGFGAFATPFDYAEQVAWAKQAGMITTMHTGGSSIPGSSGIWADHLLAVQPHVSFH
                     VNGGPVAMPDKDFPRLVEESEIALQICTAGNLRTKLLVADLARKANAFHRVLIATDTP
                     TGSGIMPLGMLYTISHLASLTDLAPELAIAAATGNNAEVYRLNSGFLRNGRDADIVLI
                     DACAGGSQGDALSALRNGDVTAVGAVVTAGVPRFVGRSRNTPATIRSVRVAECRLPMD
                     FSGADH"
     gene            3390..4772
                     /locus_tag="VGB93_11515"
     CDS             3390..4772
                     /locus_tag="VGB93_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0146747.1"
                     /translation="MRTSDQAISINPYTEETIETFSDLSDDGAEQILGAAARAFGSWK
                     MMPVARRAEIIRKAGALLRQNVDKFAKAITLEMGKTIGEAEAEVLKCAAQCEWYADNG
                     PAMLADRPMPVESGAAYISYLPLGPVLGVMPWNFPLWQAIRAAVPILLAGNAFILKPA
                     RNVLRSAFNLEDAFRESGMPDGLFSVLNISQDKISGVIADRRVAAVTVTAGVRAGAAV
                     ASQASALLKKSVLELGGSDAFIVLKDADLDRAVSVGIKARFQNNGQVCIAAKRFILEK
                     PIAAEFTERFVEGVRALKLGDPLDRATRLGPMARADLRDGLHKQVEESKHTGARLLAG
                     GHYVESRGYFYQPTIFDGVTPGMPIFDEETFGPCAALKLVNDLDEAIALTNQSEYGLS
                     SNLWTNDLDLAAKAARRIEAGGVFVNGFSATDPRVPIGGVKLSGYGRELSSFGVTEFA
                     NIQTVWRERA"
     gene            4859..6283
                     /locus_tag="VGB93_11520"
     CDS             4859..6283
                     /locus_tag="VGB93_11520"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0146748.1"
                     /translation="MSVAYQDLREFIALADQLGAVRHISGAQVFHEIGGITEVAAGLA
                     HAPALLFDNIPGYRSGFRVFTNATTNAQRAALALGLDPTLRPLDALKLWKEKRTGLKP
                     IAPTVVGDAPFMENSVRGDDVNLAVFPAPHWHPEDGGPYIGSGALIVSRDPDENWVNA
                     SIYRVQVHSRNRVTIQFDHTGRHGSMIAQKYWSKGQGCPVAIVIGQDPALFIAGFEYL
                     PGGQSEYDFAGAIKDAPIEVMKGPVTGLPVPARAEIILEGELLPFPENSLPEGPFGEF
                     TGYYAADKRPAAVMEVTGMHYRNNPILFGSPPLKPPRFHFGLPFRAAGIWANLEALGV
                     SDVVGVWQHVSQLMTVVALKQRYAGHAKRAGLIAAANSYMGRVVVVVDEDIDPSNLAD
                     VMWAVTTRSEPSESVDIIRNGWSSSLDPRLSAEARAQGATANSKMIIDACKPYHLIAE
                     YPRASALSLGAARAIEEKWGAVLR"
     gene            6321..>6916
                     /locus_tag="VGB93_11525"
     CDS             6321..>6916
                     /locus_tag="VGB93_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY0146749.1"
                     /translation="MSSKTKSADLIVALTGASGAIYCIRLLEALKETEIRTHLVMSKW
                     GIQTLKYETPHTAASVRRLADECYSDNDLGATISSGSFQTMGMVIVPCSAKTLGSIAH
                     GHGSGLIQRAADVVLKERRKLVLAVREMPLNDIHLENMLKLSRMGAVICPPVPAFYTL
                     PKTIDDIVDQTVGRIADQFGIALPLAARWAGTPPVFETA"
BASE COUNT         1397 a         1998 c         2137 g         1384 t
ORIGIN      
        1 catcgacgac tcggaagcgg cggaccatcg accagtcctt gcagcccagg agcagcggca
       61 catggtcgag ctcgtaccag gcatcaaaat cggctagcgc gctttcgggc acctcgaacc
      121 aaacggcgaa gagcagcggt gcatcgagcg cggaaacgcc gctatcgttt ggctgccgtt
      181 tgacgctggt ttcagcggca atgtagcggg tgaaaccaga gatggtgccg agcatttcgc
      241 gcgtcgtctc gctcggcttc gtcttcacct cctgataggc cggcgtcttc atcacgttcg
      301 gatctgtcat ttcataaact gcgaggaagc ccttcgcatt ttcgctgaga tcacgataac
      361 gctgcgccga aacgaaaccc ggcacgtcca tgcgcagcgg gatatgctcc tcatcatacc
      421 aattgtggaa gcgctgctcc tggccaggcg gcggcgtcat ttcagaaaac agaaccgatg
      481 caccctgcat aatatctcct tgtcgcggcg gaacgccggc gatttcgatt tgatcaggcg
      541 acttccgcgg cttcgcggac gctttcgatg agcgacgagc gcaggaggaa ggctcgcgcg
      601 cgcgccggat cagctgccgt cgcgcgcaac gcgtctaggc tcgctttgcg cgccgcggaa
      661 tctctttctt ccaagcgctt cttgttggcg atggtttgct cttgcacgaa cttcacattc
      721 agcaatcggc gccgcctcga ataatgatcg agcgccgcag ggtctttccc gcgtaacgcg
      781 acagcagaaa gcgtggcaac ggcatccgtc gcgtcgtgaa cgccgaaatt gagacccaaa
      841 ccgccgatcg gattgttgac atggccggag tcgccggcga gcagtacgcg gcccacgcgg
      901 aacgtctcgg cgacccgctg gtggacggcg taaagattct tgtgcacgat ttggtaaggg
      961 cgcccgagcg ggtagagccc ctcgaggcgc gctagcgcgc gcgcgtcgga cagcgcctct
     1021 tcatcgcttt catcgactgt ggtcgggaag acagcgcgcc agcgaccctt gccgtcgtca
     1081 ccagcgacct tgaacagatt ggcccactcg ccgatgccgg cgaagtaact gcggaaggcg
     1141 aagccgccga gggcgtgcat atcgtcgagt atggtcagaa cgctgaagcg ttccggccat
     1201 gtgtagccgt cgaaggatac gccgagcgat ttgcgcattg cgctgcggcc gccgtcggcg
     1261 gcgatgatcc aatcggcaac gagcgtttcg ggcccttcag ccgtttccac ctagacggag
     1321 acatgatccg catcttgcgt gcaggagaca gcgcgtacgc cgaaacggac gttcacaccg
     1381 gattcacgca gcttgtcgag gcccatacgc gcgagtttgt gctgttcggt ctgaacgacg
     1441 aaaggaaagc gcgtgtcgtt gcgcagaagt tcgtgatcga aggtcgcgac caaagtgcct
     1501 gtttcacggt cccagaactg aaactcgcgt gccaccagac cttccgcgat gaagcgatcg
     1561 agaagcccga tcctgtcgat catttcgagt gtcgaaggat gtgtggttga ggcgcgcggc
     1621 cgcgtatcaa tctcagtcgc ggcttcgatc aattcgacgt cgaaacccgc ttgcgtcgcg
     1681 gcaacggcgg aaacaacgcc gacgggacca ccgccgacga caagaagttt aggcgccttg
     1741 gtcaatgatc agctcctgaa aagtccatgg gtaaacggca ttcggcgacg cggacgctgc
     1801 ggatggtggc gggcgtgttg cggcttcgtc cgacgaagcg cggaacgccg gccgtaacga
     1861 cggcgccgac cgcagtgaca tcgccattgc gaagcgcgga cagagcgtca ccctgagagc
     1921 cgcctgcgca ggcatcaatg aggacaatat ccgcgtcacg cccatttcgc aggaagcctg
     1981 aattcagcct gtagacttca gcgttgtttc cggtcgctgc ggcgatcgcg agttccggtg
     2041 cgagatcggt gaggctggcg agatgcgaaa tcgtataaag catcccgagc ggcatgatac
     2101 cgctgccggt cggcgtgtcg gttgcgatca gcacgcgatg gaaggcattc gctttgcgcg
     2161 cgagatcggc aacgagcaat ttcgtgcgca aatttcccgc ggtgcagatt tgcagcgcga
     2221 tctcggattc ctcaacgaga cggggaaagt ccttgtccgg catcgcaaca gggccgccgt
     2281 tcacatgaaa cgaaacatgc ggctgcacgg caagaagatg atccgcccaa atgccggatg
     2341 agcccggaat ggatgaaccg cccgtgtgca tcgtcgtgat catgcctgcc tgcttggccc
     2401 aggcgacctg ctcggcatag tcgaagggcg tcgcgaacgc gccgaaaccg gccttggcga
     2461 gccaaacacc gtgatttctc aattcggcga agtcgctttc gttgaggccc ggctcaagaa
     2521 tgactgagcc ggcatggacc cgcatgcctc ccggacgata ggtcttgaaa cagcgcgccg
     2581 cagcaaccgc gagtgccttg acgccgaccg ggtcctttgg gcggccgggc acatggactt
     2641 cggacgccga gatcgcggtc gtcacgccgc catgcagata actttccaga tagccgaccg
     2701 tcttctgccg cggcgtataa tcgccgaagg taatatggac atggctgtcg atcaggccgg
     2761 gaatcgcggt ggcgccgtcg gcatcgatca cgacatccgc ggagctcacg tcctgcgccg
     2821 acagggtccc aaccgattgg ataaggccat cttcacaaag aattgcatcg ccgggctcaa
     2881 acggcgattt ccaatcgccg ctgccgatcc ggccaatgtt tacgatggca gtcttcatcc
     2941 gtcgctcctc gatgtcagcg caatgccggc ggtacgctaa agcccggagg gtgacgcgag
     3001 agccttctct tcgtcgccgc gcgggccgga tcgtaccaaa ggcacacacg ttttagcgcc
     3061 gcgaagcgac cgatgtcgat caaacctgtt tgtaagacgc tgccaaacag cattgcggcc
     3121 agtttcagat cggagtccgg tcatttcaag agagcacaac acgtgcggct gcacatattg
     3181 taagacgatc ttaaaatctt ggcagtcgcg ccgtcaagtt gtaggcagat taatatctaa
     3241 tcagaacaag caaggctttt aggcatgttg ccgcggacac ggccgactgt agtgccgttg
     3301 aatgatattg acatattgta tgacaatata gttgttgttt tgccgccgct gcgggtagcc
     3361 ggcctaccga gaagccagag accaagacca tgagaacgag cgaccaggcg atctcgatca
     3421 atccctatac cgaagagacg atcgagacat tttccgatct gagcgacgat ggtgccgagc
     3481 agatattggg cgccgccgcg cgcgcctttg gctcctggaa gatgatgccg gttgcgcggc
     3541 gggccgaaat catccgcaaa gctggcgcgt tactccggca gaatgtcgac aagttcgcca
     3601 aggcgatcac gctcgaaatg ggcaagacga tcggcgaggc ggaagccgag gtgctgaaat
     3661 gcgccgcgca atgcgaatgg tatgcggaca acggccccgc gatgctcgcc gaccggccga
     3721 tgcctgtcga aagcggcgcg gcttatatct cttatctgcc gttggggcct gtccttggtg
     3781 tgatgccgtg gaatttcccg ctctggcagg caatccgcgc ggccgttccg atcttgcttg
     3841 cgggcaacgc ctttattttg aagccggcgc gcaacgtcct gcgctcggcg ttcaatcttg
     3901 aagacgcatt ccgcgagagc ggcatgcctg atggcttgtt ttctgttctt aacatttcgc
     3961 aagacaagat atcgggcgtc attgctgacc ggcgcgtggc tgcggtcacg gtgacggctg
     4021 gtgtgagagc gggcgccgca gtcgcgtccc aggcaagtgc cttattaaag aaatccgtgc
     4081 tggagctcgg cggatcggac gcgttcatcg tactgaagga cgcggatctc gacagagcgg
     4141 tttcggttgg gatcaaggcg cgttttcaga acaacggtca ggtctgcatc gccgcgaagc
     4201 gcttcattct ggaaaagccg atcgcggcgg aatttaccga acgcttcgtc gaaggcgttc
     4261 gcgcgctgaa acttggcgat ccgctcgacc gcgcgacgcg tttgggacca atggcgcgcg
     4321 cggacttgcg tgatggtctt cacaagcaag tcgaagagtc aaaacacacg ggcgcccggc
     4381 ttttggcggg gggacattac gttgaatccc ggggctattt ttatcagccg acgatattcg
     4441 acggcgtcac acccgggatg ccaatctttg acgaagagac gttcggaccc tgtgctgcac
     4501 tcaaattggt aaacgatctg gacgaagcga ttgcgctgac caatcaaagc gaatatggcc
     4561 tcagcagtaa tttgtggacg aacgatctcg atctcgcggc aaaggccgcg cggcgcatcg
     4621 aggcaggcgg cgttttcgtc aatgggttct ccgccaccga tccgcgtgtt ccaatcggcg
     4681 gtgtcaagct gagtggttac gggcgagaac tctcctcgtt tggcgtgacc gaatttgcaa
     4741 atatccagac cgtctggcgg gagagagcct aggttgcgac gggtgaattt gccggaaggt
     4801 ggaatggttt ttgcggcatt agcgcccggt ttgaatctaa cgataaaatt ggagcgatat
     4861 gagcgtcgcc tatcaggacc ttcgggaatt catcgcgctg gccgatcagt tgggcgcagt
     4921 acggcacatt tccggcgcgc aagtttttca cgagatcggc ggcattaccg aggttgctgc
     4981 gggccttgcg cacgcgccgg cgcttctctt cgataacatt ccgggctacc gcagcggatt
     5041 tcgtgtcttc acaaacgcga cgacgaacgc gcaacgcgca gcgctggcgt tggggctcga
     5101 tccgacgtta cggccgctcg atgctttgaa gctctggaag gagaagcgca ccggattgaa
     5161 gccgattgcg ccgaccgtcg tcggcgacgc cccgttcatg gagaattcgg tgcggggcga
     5221 tgacgtcaat ctcgctgttt tcccggcgcc gcattggcat ccagaggacg gcgggccgta
     5281 tatcggttcc ggcgcgctga tcgtcagccg cgatccagat gaaaactggg tcaacgcttc
     5341 gatctaccgc gtgcaggttc attcgcgtaa tcgcgtgacc atccagttcg atcacaccgg
     5401 gcgccacggc tcgatgattg cgcaaaaata ttggtcgaag gggcagggat gccctgtcgc
     5461 aatcgtcatc gggcaagatc cggcgctctt catcgcaggc tttgaatatc tccctggggg
     5521 acagtcggag tatgattttg ccggcgctat taaagacgcg ccgatcgagg tgatgaaggg
     5581 gcctgtcact ggcctgccgg tcccggcgcg ggcggagatc atccttgagg gcgaattgtt
     5641 gccattcccg gagaacagtc ttccggaagg cccgtttggc gagttcaccg gctattacgc
     5701 ggctgacaaa aggccggccg ccgtgatgga ggtgacgggc atgcattatc gaaacaaccc
     5761 gattcttttc ggttcgccac cattgaagcc gccaaggttt cactttggcc tgccgttccg
     5821 tgctgccggt atttgggcaa atctcgaagc gctgggcgtc tcggacgtcg ttggcgtctg
     5881 gcaacacgtc tcgcagctca tgaccgtcgt ggccttgaag cagcgctacg cgggtcatgc
     5941 caagcgcgcg ggtctcattg ccgccgcgaa cagctatatg ggtcgcgtcg tcgtggtggt
     6001 ggatgaagat atcgacccct ccaatctcgc cgacgtcatg tgggcggtca cgacgcggtc
     6061 ggagccttcg gaatccgtcg atatcatccg caacggctgg agttcttcgc tcgatccgcg
     6121 cctgagcgcg gaggcacggg cgcagggcgc cacggccaac tcgaaaatga ttatcgacgc
     6181 ctgcaagcct tatcatttga tcgccgaata tccccgggcg agcgccctct ctctaggggc
     6241 ggctcgggcg atcgaggaaa aatggggcgc ggtgttacgc tgaccggaat ttattgagcc
     6301 gctgtcatga ttggaaagcg atgagcagca aaacgaaatc cgccgatctt atcgtcgccc
     6361 taaccggggc cagcggcgcg atctattgta tccggcttct cgaagccctg aaggaaaccg
     6421 agatacgcac tcatctggtg atgagcaaat ggggtatcca aactctcaaa tacgagacgc
     6481 cccacacggc ggcttcggtg cggcgtttgg cagatgaatg ctattcggac aatgacctcg
     6541 gtgcgacgat ttcgagcgga tcgtttcaga cgatgggcat ggttatcgtg ccttgttcgg
     6601 ccaagaccct tggctccatc gcgcacggac acggcagcgg gctcatccag cgtgccgccg
     6661 atgtcgttct gaaggaacgc cgcaagcttg tccttgcggt gcgggaaatg ccgctgaacg
     6721 acatccatct cgaaaacatg ctgaagcttt cccggatggg cgcagtgatc tgcccaccgg
     6781 tgccggcgtt ttatacgctc cccaagacga tcgatgatat cgtcgatcaa accgtcggac
     6841 ggatcgccga ccagtttggc atcgcgcttc ccttggcggc gcgctgggcc gggacgccgc
     6901 cggtctttga aaccgc
//
LOCUS       DASWOG010000563         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_32293,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000563 DASWOG010000000
VERSION     DASWOG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32293"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGB93_11530"
     CDS             complement(<1..409)
                     /locus_tag="VGB93_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146750.1"
                     /translation="MSKVATHRRSNLWRTSVFGALAALSIATAKAEPAQIIRPGNNIF
                     PESVTSTADGTLYVGSFAAGGVFRVPQGASHADVWIKPGAFGTRSLLGVHADEKSQTL
                     WVCSNDLSAIGLPGPSKVPGAWLRGFDLKTGVGK"
     gene            782..1381
                     /locus_tag="VGB93_11535"
     CDS             782..1381
                     /locus_tag="VGB93_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0146751.1"
                     /translation="MMSTISRLRAFFFIILLALPISACGYNQIPTLQENAKAKWADVQ
                     NNYQRRADLIPNLVATVQGYAKQEKDVLVQVTEARAKATQVHVDSSDLTDPAKLKAFQ
                     DAQAQLSGALGHLLAISENYPDLKSNQNFLALQSQLEGTENRIAVSRRDYIEAVRQYN
                     TALVTFPGVLWASTLFKNSKPMAEFTANDNAQTAPQVKF"
     gene            1404..2207
                     /locus_tag="VGB93_11540"
     CDS             1404..2207
                     /locus_tag="VGB93_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0146752.1"
                     /translation="MVSHRAKVSFVRPGARAIFTIAALACALAFVSVAAFAYNFPPLT
                     GRVVDQANIIPDATRQEITQKLADLEQKSGIQLVVATVNSLEGGDIETYANELFRVWK
                     LGEKDKNNGVLLLVAPKEHKVRIEVGYGLEGTLTDALSKIIIVNAITPSFKKGDFGGG
                     ISRGVDDIITTLTTDSSDWQPKPQVRADQDDNSSAIGWVLIVGAIFLVVMLGVSPGFR
                     WFFFNVLLNALLSSGGSRGGGDSWGGGGGGGGFSGGGGSSGGGGASGDW"
     gene            2207..2815
                     /locus_tag="VGB93_11545"
     CDS             2207..2815
                     /locus_tag="VGB93_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0146753.1"
                     /translation="MISADDRARISAAIHAAEAKTSGEIVCVLAESSSQAGALPVLIA
                     AIVALALPWLLISFTNFAVERILLLQILVFVLLTFLLLLPPVYLALLPPRVKRAAAYR
                     LATEQFVVRGIARKKDRSGILIFVSLAERYARIIADEGVAARTSQAEWQAAIDVLVSH
                     MRQDQVADGFIAAIGACGDVLAAHFPHTQAGRDELPDRIYVI"
     gene            2905..3717
                     /locus_tag="VGB93_11550"
     CDS             2905..3717
                     /locus_tag="VGB93_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146754.1"
                     /translation="MDLQLNGKRALVTGSSIGIGEAIAHALAAEGVAVAVHGRNRERT
                     GLVAKEIMAAGGKAVVVLGDLTNDEDVGRMVDEAQRLLGGVDILVNNAGGSGEKQNWE
                     STPIADWIAAFDRNVLAAVRVTGLLLPKMRAARWGRVVNVSSLAGMMPPATGPDYSAA
                     KAAMNNMTVSLSKAAAADGVTVNAISPGPILTPKLEAAFRKIGASNGWVDGAAAWPDI
                     ERVVLPHVVHVPIGRVGRAADIAHEVAFLCSPLAGYITGVDLRIDGGTLPSL"
BASE COUNT          738 a         1175 c         1237 g          855 t
ORIGIN      
        1 tttttccgac gccggtcttg agatcgaagc ccctgagcca agcaccgggc accttgctcg
       61 gcccagggag gccgatcgcg gaaagatcat tggagcagac ccagagcgtc tgcgactttt
      121 cgtcggcatg cacaccaagc aacgagcgtg tgccgaatgc tcccggcttg atccatacgt
      181 ccgcgtggct cgcgccctgc ggaacgcgga agacgccgcc tgccgcgaag cttccgacat
      241 agagggtgcc atccgccgtc gacgttacgc tctcggggaa aatattgttg ccgggccgga
      301 tgatttgggc aggctcggcc tttgcagtgg cgatgctcag cgctgcgagc gcgccgaaaa
      361 cgctagttcg ccacaaattt gagcgccggt gtgtcgcgac tttggacatt agctcccccc
      421 gtccaatgcg agggtcatgt atgcaccggc ctggcgcgcc gccagccttg tctggcaagt
      481 ttctaatcgc tgtccggcat gaagaaaacg tgacgacaaa tgcgcgttat ctcatgcacg
      541 gccatcgtgt gagcgcccgc atctttggca agtcgcatcg catgcaatgt caagcggcag
      601 cgatcatccg gaaaatgcca tgtttacttt tcggcgtctt ttgtcgcacc gcgtgtgacg
      661 gcgcaccatg gtcacatgct atagctggcg ccgcggccgt gacccaattg acacggcggt
      721 gcgaaccttg gcttaatccg cgccgcaaag tcagccgttc attcttcatc acgaggcctt
      781 tatgatgtct acgatttcgc gtctgcgcgc ttttttcttc atcattttgc tcgcgcttcc
      841 gatttcggcc tgcggctaca atcagattcc gaccttgcag gaaaatgcga aggccaaatg
      901 ggccgatgtg caaaacaatt atcagcgccg cgctgacctc attcctaacc ttgtcgcgac
      961 cgtccagggt tatgccaagc aggaaaagga cgtgctggtt caggtcacag aagcccgcgc
     1021 caaggcgacg caagtgcatg tcgattcttc ggacctcacc gatcctgcaa aactcaaggc
     1081 tttccaggat gcgcaggcgc aattgagcgg cgcgcttgga catctgctgg cgatcagcga
     1141 gaattatccg gatttgaagt cgaaccagaa ttttctcgca ttgcaatcgc agcttgaggg
     1201 cacggaaaac cgcatcgccg tctcgcgtcg cgattatatc gaggcggtgc ggcaatataa
     1261 tacggcgctt gtgacattcc ccggggttct ctgggcctcg accctgttca aaaacagcaa
     1321 gccgatggcg gaattcacgg cgaacgacaa tgctcagacc gcgccacaag tgaaattcta
     1381 actcctgcgc cggaggcgct ggaatggttt cacaccgcgc aaaagtctcg tttgtccggc
     1441 ccggtgcgcg cgcgattttc acaatcgcgg cactcgcctg cgccttggct ttcgtcagcg
     1501 ttgcggcgtt cgcctataat tttccgccgc tcaccggccg cgtcgttgat caggccaata
     1561 tcatccccga cgcgacgcgg caggagatca cgcagaagct cgccgacctc gaacagaaat
     1621 ccggcattca acttgtcgtt gcgacggtca attcgctcga aggcggcgac atcgagacct
     1681 atgccaacga actctttcgt gtctggaaac ttggcgagaa agacaagaat aacggcgtgc
     1741 tgctgctcgt cgcgccgaag gagcacaaag tccggatcga ggtcggctat gggctcgaag
     1801 gtacgctcac cgatgctctg tcgaagatca ttatcgtcaa tgcgatcacg ccgtcgttca
     1861 agaaaggcga tttcggtggc ggcatttcgc gtggcgtcga cgacatcatc acgacgctga
     1921 cgacggattc gtctgactgg cagccgaagc cgcaggtacg tgcggatcag gacgacaatt
     1981 caagcgcgat cggttgggtg ctgattgtgg gtgccatctt tctggttgtg atgctcggcg
     2041 tttcacccgg cttccgttgg tttttcttca atgttctttt gaatgcgctg ttaagctcgg
     2101 gcgggtcgcg cggcggtggc gattcatggg gcggcggcgg tggtggtggc ggcttctccg
     2161 gcggtggcgg ttcatcgggt ggtggcggcg cctcgggaga ttggtgatga tctcggcaga
     2221 tgaccgcgca cgtatttcgg cagcgatcca cgcggcggag gcaaagacct cgggcgagat
     2281 cgtttgcgtt ctcgccgaaa gctcgtctca ggccggcgcg ctgccggttc tgatcgcggc
     2341 gatcgtcgcg ctggctcttc cctggctgct catcagtttt acgaattttg ctgtcgaacg
     2401 cattctgctg ctgcaaatcc tcgtctttgt gctcctcacg ttcctgctcc tcttgccgcc
     2461 cgtctatctc gctctcctgc ctccgcgggt aaagcgggcc gcggcgtatc gactcgcgac
     2521 cgaacagttc gtcgttcgcg gcatcgcccg caaaaaggat cgcagcggca ttttaatttt
     2581 cgtctcgctg gcggagcgct atgcgcggat catcgccgac gagggcgtcg ccgcccgcac
     2641 gtcgcaggcc gaatggcagg cggcgatcga cgtgcttgtc tcgcatatgc gtcaggatca
     2701 ggttgccgac ggcttcattg ccgcgatcgg cgcttgcggc gacgtgctag cggcgcattt
     2761 cccgcacacg caggcggggc gggacgaatt gcccgaccgg atttacgtaa tctgatcgca
     2821 tctgtgcacc aaggattgac gggttgaacg gtcgccggct tcctggcgcg tatactcgcc
     2881 cttagaggtc ctttggagga actgatggat ctgcagttaa acggcaagcg cgcactggtg
     2941 acgggcagta gcatcgggat tggagaggcc atcgcacatg cgctggcggc tgagggcgtg
     3001 gccgtggccg tgcatggacg gaatcgagag cgcaccggtc tggttgcgaa ggaaatcatg
     3061 gccgcgggcg gtaaggcggt cgtcgtgctc ggagatctga cgaacgacga ggacgtcggc
     3121 cgtatggttg acgaggccca gcgcctgctc ggcggcgtcg atattctcgt caataacgct
     3181 ggcgggtccg gcgagaagca gaactgggag agcacaccta ttgcagattg gattgctgcg
     3241 tttgatcgca acgtgctcgc cgcggttcgt gttaccggtc tcctgttacc gaagatgcgg
     3301 gcggcgaggt ggggccgcgt ggtgaacgtg tcgagcttgg ccggaatgat gccgcctgcg
     3361 accggtcccg attattccgc cgccaaagca gcgatgaata acatgactgt ctctctctcc
     3421 aaggctgcag ccgctgacgg cgtgacggtg aatgctattt cgccgggccc gatactcaca
     3481 cccaaactgg aagctgcctt ccgcaagata ggggcatcga acgggtgggt agatggcgcg
     3541 gcggcctggc cggacataga gcgtgtcgta ttgcctcatg tcgtccatgt gccgattggc
     3601 agggttggcc gcgccgctga catcgcgcac gaggtcgcct tcctgtgtag cccgctggcc
     3661 ggctacatca ccggcgtcga tctgcggatc gatggtggga cgttgccaag cctgtgagtc
     3721 tctcgccacg ctggctcgtc aaaacgcgcg tttagcgcca ctagacgagg cccgtccggg
     3781 catagaaact ggcgcctgca atgcccatct taggcttcgc cggccgttta tccaaaacgt
     3841 cgaagatttt cccgattcgg agttccgtaa tgactgaacc cgtaacgcgt gcgctcaccg
     3901 acgcgccgat cgcgccgcgc ggcccgagcg atcaggcgaa ttataacgtt ctcgtcgtcg
     3961 gcggtggcga ttcagggctt gagcttgcga cccagctcgg caacc
//
LOCUS       DASWOG010000564         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_307969,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000564 DASWOG010000000
VERSION     DASWOG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307969"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VGB93_11555"
     CDS             complement(<1..714)
                     /locus_tag="VGB93_11555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0146755.1"
                     /translation="MDEAGSELVFVALGGLGEIGMNVALYGVGPQHKRKWLMVDCGIA
                     FSDEAGVEIVLPDLTFVEKIKRDLLGLIITHAHEDHIGAVAELWPRFQCPVFATPFAA
                     ALLAARLEGIPNAPEIKISTVKQGGRVALDPFDVELIPVAHSIPESCALAIRTPHGTV
                     VHSGDWKIDPEPGLGLPTDSARLTAIGDEGVLALVCDSTNILRDGISPSEAEVALALT
                     EVIGGATARVVVTTFASNVA"
     gene            complement(728..1507)
                     /locus_tag="VGB93_11560"
     CDS             complement(728..1507)
                     /locus_tag="VGB93_11560"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0146756.1"
                     /translation="MNFSAGAAETFTLADAAVAAGCQLHVFQTVGSTNDAAFDAARDG
                     ADRTWIVARAQTLGRGRHGRQWQSPPGNLYASLLLIDAVEPRSAPQLGFVAGVALARA
                     LRHLMPDVSRIRVKWPNDILLDGAKLAGILVEGRMLSTGAYAVVVGFGVNCRSRPADL
                     PYPAAALAETDAVRNKAEDVLALLSEEMARGLEVLERGFDAIRAEWLSLAAGVGAPIK
                     VRTATREFEGVFQSIDATGRLLLTEANGTHAIEAGDVLLGH"
     gene            complement(1504..2937)
                     /gene="nuoN"
                     /locus_tag="VGB93_11565"
     CDS             complement(1504..2937)
                     /gene="nuoN"
                     /locus_tag="VGB93_11565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY0146757.1"
                     /translation="MSTFGFTFALSHCLPEAILTVGVLALILVGAIRERAEFIITELA
                     VGVLGLALLSLFSGKANAVLFGGAFIDDGFGRFAKGMCYIGSIVTLLISHHFLVQEKI
                     NKFEFPVLVLLSTLGMSLLISANGLIALYLGLELMSLALYVIAAFHRDDAKASEAGLK
                     YFVLGALSSGMLLYGASLLYGFAGTVSFAGIASAVTGIPSLGVVFGLVFLMAGLAFKI
                     SAVPFHMWTPDVYQGAPTPVTAFFATAPKIAAFAITVRVIISAFPGVAVQSQQIVIFI
                     SILSMALGSFAAIGQQSFKRLMAYSSIGHVGFALVGLAAHNQAGVAAVLFYLGIYLVM
                     VLGTFAAILSMQVSGKPVENVSDLSGLARSDGAMAFFLSMLMFSLAGIPPLAGFFAKF
                     YVFDAAVRAHLYGLAVIGLVTSVVAAYYYLRIVKIMYFDEPATAFDKAPTVLRVVLAV
                     AGIFVVLMFVYPAPFFAATVAAAKTLF"
     gene            complement(2934..4457)
                     /locus_tag="VGB93_11570"
     CDS             complement(2934..4457)
                     /locus_tag="VGB93_11570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0146758.1"
                     /translation="MFGFGILSGLVVLPLVGAAFILTLRGDDKATQNNARWAALATAI
                     ITFLFSLVVCSKIDFSSSAFQLVEEHRWFGDGLVYKLGLDGLSAPFILLTTFLKPFCI
                     LASWRSVQFRVKECMIAFLILETLLLGAFSALDLVLFYLFFEGGLIPMFLIIGIWGGK
                     RRVYASFKFFLYTLLGSVLMLIAIMAMYNFAGTTDITILLKTHFPPQMQTWLWLAFFA
                     SFAVKLPMWPVHTWLPDAHVEAPTGGSVDLAAILLKLGGYGFMRFSLPMFPEASAHFA
                     PLIFALSVIGIVYTSLVALVQTDMKKLVAYSSIAHMGFVTIGLFSMTQQGVQGALFLM
                     ISHGLVSAALFLSVGVIYDRMHTREIAAYGGLVARMPLYALAFMLFTMANIGLPGTSG
                     FIGEFLSTLGAFKVNSWVGAFAALGTILSACYALYLYRRIIFGVLDKPSLKGITDLSL
                     REIVTLAPLFLIIIYYGVHPGPLLDNTAPSVTNLVQGIELRLAEAHERTLQVVGLSQ"
     gene            complement(4457..>5066)
                     /locus_tag="VGB93_11575"
     CDS             complement(4457..>5066)
                     /locus_tag="VGB93_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0146759.1"
                     /translation="AGALVDHAHDDHAHAPHESPPVVLVPLFVLAFGALVAGFVFKHF
                     FIGEGGPDFWKSALYYGPANHILQAMEHVPALVSLGPTLMMVGGFFVALYVYILAPGT
                     AQKWADAMPGLYRFLLNKWYFDELYDRIFVRPAFWLGNLLWKGGDENIIDRFGPNGVA
                     ARVVDVTNRVVRLQSGYLYHYAFAMLIGVAALITYYLVGSLR"
BASE COUNT         1308 a         1489 c         1482 g          787 t
ORIGIN      
        1 cgcgacattc gaggcaaagg tcgtgacgac gacacgcgcc gtcgcgccac cgatcacctc
       61 tgtcagtgcc agtgccactt cagcctctga aggactgata ccgtcgcgca gaatattggt
      121 ggaatcgcag acgagcgcga gcacaccctc atcgccgatt gccgtcagcc gcgccgaatc
      181 cgtcggcaaa cccagccctg gctccggatc gatcttccaa tcgccagaat gaacgaccgt
      241 gccatgtggc gtacgaatcg ccagcgcgca gctttccggt atcgaatgcg caacggggat
      301 caattccaca tcgaacggat caagcgcgac gcggccgccc tgcttgaccg tcgagatctt
      361 gatctccggc gcattgggaa tgccctcgag ccgtgcggcg agaagcgcgg cggcaaatgg
      421 tgttgcgaag accggacatt gaaaacgcgg ccagagttcc gcgacagcgc cgatatggtc
      481 ttcatgcgcg tgcgtgatga tcaggccaag aagatcgcgt ttgatttttt cgacaaaagt
      541 gaggtccggc agaacgatct cgacaccggc ctcatcggaa aaagcaatgc cgcaatcgac
      601 catcagccat ttgcgcttgt gttgcggacc gacgccataa agggcgacgt tcatgccgat
      661 ttcgccaagg ccgcccaacg ccacgaagac cagttcactt cccgcttcgt ccattcacct
      721 gtcctgacta atggccgaga agaacgtcac cggcctcgat cgcatgcgtg ccattcgcct
      781 cagtgaggag caagcggccc gtcgcgtcga ttgattgaaa aacgccctcg aattcccgcg
      841 tggctgtgcg caccttgatc ggcgcgccaa ccccagccgc aaggcttaac cactcggcgc
      901 ggatggcgtc aaagcctcgt tccaaaactt ccagcccgcg cgccatttcc tctgagagca
      961 aagcaagaac atcctcggct ttgttgcgca ccgcgtccgt ctcggcaagt gctgcggctg
     1021 gataaggtag atcggctggg cgcgaccggc aattgacgcc gaacccaacg acgacggcat
     1081 aggcgcccgt cgaaagcatg cgcccctcaa ccaggatgcc agcgagcttt gcgccatcga
     1141 gcagaatatc gttgggccac ttcacacgaa ttcgtgaaac gtcgggcatg agatgtcgca
     1201 acgcacgcgc cagcgcgacg ccggcgacga agccgagttg cggcgcggag cgcggctcga
     1261 ccgcgtcgat gagcaacaga cttgcataga gattgcccgg cggcgattgc cattggcggc
     1321 cgtgacggcc acggcccaac gtctgcgcac gggcgacgat ccaggtgcga tccgcgccgt
     1381 ctcgcgccgc atcaaatgcg gcatcgttgg tcgagccgac cgtttgaaag acgtgaagct
     1441 gacagcccgc cgcgactgcc gcatcagcaa gagtaaacgt ctcggcagct ccggcggaaa
     1501 agttcaaaac agcgtcttgg cggccgcgac cgtcgctgca aagaagggcg caggatagac
     1561 gaacataagc acgacaaaaa tccccgcgac ggcgaggacc acgcgcaaga ccgtcggcgc
     1621 tttatcaaac gctgtcgccg gttcatcgaa atacatgatc tttacgatgc gcagataata
     1681 ataagccgcc acgacgctgg tgacgaggcc gatcaccgcg aggccgtaaa gatgcgcccg
     1741 taccgcagca tcgaagacat aaaatttcgc gaagaacccg gccagcggcg gaataccggc
     1801 gagtgaaaac atcagcatcg ataagaagaa cgccatggct ccatcggagc gcgcgagtcc
     1861 ggaaagatcg ctgacattct cgaccggctt gccgctgacc tgcatcgaaa ggatcgccgc
     1921 gaatgtcccg agaaccatga cgagatagat gccgagatag aacaaaactg ctgccacgcc
     1981 agcctgatta tgcgcagcaa ggccgacgag cgcgaagccg acatggccga tcgaagaata
     2041 ggccatcagc cgcttgaaac tctgctgtcc gatcgcggca aaggatccca gcgccatcga
     2101 caggatcgaa atgaagataa cgatctgctg cgattgcaca gcaacgcccg gaaaggcgct
     2161 gataatgaca cggacggtga tcgcgaaggc cgcgatcttc ggcgctgtcg cgaaaaaggc
     2221 cgtcaccggc gtcggcgccc cctgatagac atccggtgtc cacatatgga agggcactgc
     2281 ggaaatcttg aaggccaatc cggccattag gaagacgagg ccaaacacga cgccgagcga
     2341 cggtatgccc gtcaccgccg aagcgatccc ggcgaacgaa acggtgccgg cgaagccata
     2401 gagcagcgac gccccgtaaa ggagcatgcc ggacgaaagc gcgccgagga cgaaatattt
     2461 aaggccggct tccgaggctt tcgcatcgtc gcgatgaaac gccgcgataa cataaagcgc
     2521 gagcgacatc agttcgaggc cgagataaag cgcgataagc ccgttcgctg atatgagcaa
     2581 cgacatgcca agagtcgaaa gcaatacaag cactggaaat tcaaacttgt tgattttttc
     2641 ctgcacaaga aaatgatgcg agatcagcag cgtcacgatc gagccgatat aacacatgcc
     2701 cttcgcgaag cgcccgaagc cgtcgtcgat gaatgcgcca ccgaacagca ccgcattcgc
     2761 cttgccgctg aaaagcgaca agagcgcaag gccgaggacg cccaccgcga gttccgtgat
     2821 gatgaattcg gcacgttcgc gaatggcacc gaccaggatc agcgccagaa cgccgacggt
     2881 caggattgct tcaggcaggc aatgcgaaag cgcgaaggta aaaccgaacg tactcattgg
     2941 gacagaccca ctacttgcag cgtccgttca tgcgcctcgg ccagacgcaa ttcgatgccc
     3001 tgaacgagat ttgtgaccga aggcgcggtg ttatcgagga gcggccccgg atgcacgccg
     3061 taatagatga tgatcagaaa gagcggtgcg agcgtcacga tctcgcgtag cgagagatca
     3121 gtaatgccct tcaggcttgg cttgtcgagc acgccgaaaa taatgcgccg gtagaggtaa
     3181 agcgcatagc acgctgaaag aatggtgccg agtgccgcga aggcgcctac ccacgaattc
     3241 accttaaagg caccaagcgt tgacaggaat tcgccgataa agcccgacgt acccggcaga
     3301 ccgatattgg ccatagtgaa aagcatgaag gcgagagcgt aaagcggcat acgcgcgacg
     3361 aggccaccat aggcggcgat ctcgcgtgtg tgcatccggt cgtatatgac gccgacgctg
     3421 aggaacaagg cggccgaaac gagcccgtgc gagatcatca ggaagagcgc gccctgcaca
     3481 ccctgctgcg tcatggagaa caggccgatg gtgacaaagc ccatatgcgc gatcgatgaa
     3541 taagcgacaa gcttcttcat gtcggtttgc accaacgcga ccagcgaggt gtagacgata
     3601 cctatgaccg atagcgcgaa gatgagaggc gcaaaatgcg cggacgcctc cgggaacatc
     3661 ggtagcgaaa aacgcataaa gccgtatccg cccaatttca gcaatatggc cgcgagatcc
     3721 accgacccac cggtcggcgc ctcgacatgc gcgtccggca gccaagtgtg gaccggccac
     3781 atcggcagtt tcaccgcgaa agaggcgaag aaggcgagcc acagccaagt ctgcatctgg
     3841 ggcgggaaat gagtcttcaa caggatggtg atgtctgtcg tgccggcaaa attgtacatc
     3901 gccatgatgg cgatgagcat caaaaccgag ccgagcagcg tgtaaaggaa gaatttgaag
     3961 ctcgcataaa cgcgccgttt accgccccag atcccgatga tgaggaacat cgggatcaaa
     4021 ccgccctcga agaaaagata gaaaagcacg agatcgagcg cggagaacgc gccgagaagc
     4081 agcgtctcga ggatcagaaa cgcgatcata cattccttca cccggaactg aaccgaccgc
     4141 caggacgcga ggatgcagaa aggtttgagg aaggtcgtca gcaagatgaa aggcgccgac
     4201 aaaccgtcga ggccgagttt atagacaagg ccgtcgccaa accagcgatg ttcctcgacg
     4261 agttgaaagg ccgaggaact gaaatcaatc ttcgaacaaa cgaccagcga gaacaggaag
     4321 gtgatgattg ctgtcgccag cgccgcccag cgcgcgttgt tttgcgtcgc cttatcatcg
     4381 ccgcgcaggg tcaggatgaa ggccgcgccg acgagcggca gaacgacgag ccccgagagg
     4441 atgccaaaac cgaacatcag cgcaggctcc ccacgaggta ataggtgatg agcgccgcga
     4501 cgccgatcag catggcgaac gcgtaatgat aaagatagcc gctctgcaac cgcaccacgc
     4561 gattggtgac gtccacgacg cgtgccgcaa cgccattggg accgaaacga tcgatgatgt
     4621 tctcgtcgcc gcctttccac aacagattgc cgagccagaa cgccggacgc acgaagatgc
     4681 ggtcataaag ctcgtcgaag taccatttgt tgagcagaaa acgataaagc ccgggcatcg
     4741 cgtcggccca tttctgcgct gtaccaggcg caagaatata aacatagagc gcaacgaaaa
     4801 agccgccgac catcatgagg gtcgggccaa gactcacgag tgcgggtaca tgctccatcg
     4861 cctgaaggat atggtttgcc gggccataat aaagcgctga cttccagaag tcgggaccgc
     4921 cctcgccaat gaagaaatgc ttgaatacaa aacctgccac cagcgcgccg aaagcgagga
     4981 cgaaaagcgg gaccagcacc accggcggcg attcatgcgg agcatgcgcg tgatcatcgt
     5041 gggcatgatc gacaagagcc ccagca
//
LOCUS       DASWOG010000565         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_214951,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000565 DASWOG010000000
VERSION     DASWOG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214951"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGB93_11580"
     CDS             <1..278
                     /locus_tag="VGB93_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY0146760.1"
                     /translation="TKSTLAKATLASSLAAAVALGAVQAQADTKKSEKCFGVALKGQN
                     DCAAGAGTTCAGTQAIDYDGRYFRFVPKGTCTAIKTPKGTGSRNAKL"
     gene            complement(293..1918)
                     /locus_tag="VGB93_11585"
     CDS             complement(293..1918)
                     /locus_tag="VGB93_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0146761.1"
                     /translation="MSDAPLLDVRDLRVVFQQDGKEFEAVRGISFSVARGRTLAIVGE
                     SGSGKSVTAMSILRLLPPAAVVSGTALLKGEDILKLSERRLRGIRGAAITMVFQEPMT
                     SLNPLHTIERQVGETLELHAGVKGAALRRRIIELLTEVGIPDPAQRLSAYPHQLSGGQ
                     RQRVMIAMALANRPDLFIADEPTTALDVTVQAQILTLLKDLQQRYGMAMLFITHDLNI
                     VRKLADDVAVMQRGEIVEAGSVAEIFKSPQHPYTKALLAAEPKGTPPQSDPSAPVVLE
                     AQDIRVHFPIRRGLLRRTVGAVKAVDGISLALRQGETLGIVGESGSGKTTLGLAVLRL
                     IRSQGPIVYLGRRIDGLSFRAMRPLRRDMQIVFQDPYGSLSPRLSIADIVAEGLIAQG
                     ARLSLDERREIVAKALADTGLDPAAMDRYPHEFSGGQRQRVAIARAMVLEPKFVVLDE
                     PTSALDMSVQAQIVDLLRQLQQKRDLAYLFISHDLRVVRALANEIIVMRHGKVVEQGA
                     ASEIFANPQSDYTRALFAAAFAIEADRSGVVAQ"
     gene            complement(1915..2419)
                     /locus_tag="VGB93_11590"
                     /pseudo
     CDS             complement(1915..2419)
                     /locus_tag="VGB93_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174154.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          512 a          759 c          734 g          414 t
ORIGIN      
        1 cgaccaaatc cacgctggcc aaggcgaccc tggcctcttc ccttgccgct gccgtcgcat
       61 tgggagccgt tcaggcgcag gccgacacca aaaaatccga gaaatgcttt ggcgtcgcgc
      121 tgaagggcca gaacgattgc gccgccggcg ccggcaccac ttgcgccggg acgcaggcga
      181 tcgactacga cggccgttat ttcaggttcg tccccaaagg cacctgcacc gcgataaaga
      241 cgccgaaagg cacaggctcc cgcaacgcaa aattatgagg ccacgtactg tactattgcg
      301 cgaccacgcc gctgcggtcg gcctcgatgg caaaggccgc agcgaacaat gcgcgcgtat
      361 agtcgctctg cggattggcg aaaatctcgc tcgcggcgcc ctgctccaca accttgccat
      421 gacgcataac gatgatctcg ttggcaagcg cgcgcacgac gcgcagatcg tggctgataa
      481 acagataggc gagatcgcgc ttctgctgca attgccgcaa tagatcgacg atttgcgcct
      541 gcaccgacat gtcgagcgcc gatgtcggct cgtcaagcac aacgaatttc ggttccagca
      601 ccatcgcccg cgcgatcgcg acgcgttggc gctggccacc tgagaactcg tgcggataac
      661 ggtccattgc cgccggatcg agccccgtgt cggcgagcgc cttggcgacg atttcgcgcc
      721 gctcgtcgag actgagacgc gcgccttgcg cgatcaggcc ttcggcgacg atatcggcaa
      781 tcgagagccg cggcgacagc gagccgtatg ggtcttgaaa cacgatctgc atatcgcgcc
      841 gtagcggccg catggctcga aatgacaacc cgtcgatgcg gcggccgaga tagacgatcg
      901 gcccctgcga acggatgagc cgcagcactg cgaggccgag ggtggtcttg ccggatcccg
      961 actcgccaac gatgccaagt gtctcacctt gccgcaaagc gagcgagatt ccatcgacag
     1021 ccttcaccgc gccgactgtc cgtcgcagca ggccgcgccg gatcgggaaa tggacgcgga
     1081 tgtcctgtgc ttcgagtacg acgggcgccg acggatcgct ctgcggcggt gtccccttcg
     1141 gctcggccgc aaggagcgct tttgtataag gatgctgcgg cgatttgaaa atctccgcga
     1201 cgcttcccgc ctcgacaatc tcgccccttt gcatcaccgc gacgtcgtcc gcgagcttgc
     1261 ggacaatgtt gagatcgtgc gtgatgaaca gcatggccat gccgtagcgc tgctgtaaat
     1321 ccttcaacaa ggtcagtatc tgcgcctgga cggtgacatc aagcgccgtc gtcggttcat
     1381 cggcgatgaa gagatccggc ctgttcgcaa gcgccatcgc gatcatcaca cgctgacgct
     1441 gaccgccgga gagctgatgc ggataggccg acagccgctg cgccgggtcg ggaataccaa
     1501 cctcggtgag caattctatg attcgccgtc gcaaagcggc acctttgacg ccggcgtgta
     1561 gttcgagagt ttcgccgacc tgccgctcga tcgtatgcag cggattgagc gaggtcatcg
     1621 gctcctgaaa gaccatcgtg atcgcggcgc cgcgaatgcc gcgcagccgt ctttcactca
     1681 atttcaggat gtcttcgccc ttgagcagcg ccgtgccgga aaccacggcc gccggcggca
     1741 gaagccgcag gatggacatc gccgtcacag acttgccgga gccggattcg ccgacaattg
     1801 ccagcgtgcg cccgcgcgca acggaaaatg aaatgcctct gacagcttcg aattccttgc
     1861 cgtcctgctg gaagacgacg cgcagatcac ggacatcgag gagcggcgcg tcactcatgc
     1921 gatcgtcttt cgcggatcga aggcgtcgcg caccgcctcg ccgatgaaga tcaaaagcga
     1981 aagcatgatc gcgatgacga agaagcccgt aaggccaagc cacggagcct gaaggttcga
     2041 ctcgccttcg agcagcagtt cgccaagaga agccgaaccc gcaggcagac cgaagcctag
     2101 gaagtccagc gctgtcaaag tcgagatcga gccgttgagc acgaagggta gaaacgtcag
     2161 cgtcgcgacc attgcgttag gcagcacatg cttcaccata attcgcgcat tgctgaggcc
     2221 gagcgcgcgc gctgccgtca cgtattcaag cttgcgcgcg cgcagaaatt ccgcgcgcac
     2281 gacatgcacg aggttgaccc acgaaaacag cagcagggtg ccgagcagga tgccaaagcc
     2341 gggcgtgatg atttaggata tcatgatcag caaatagagc tgcggcacgg aagaccagat
     2401 ttcgatcagc cgctgcatg
//
LOCUS       DASWOG010000566         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_214988,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000566 DASWOG010000000
VERSION     DASWOG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214988"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1046
                     /gene="murI"
                     /locus_tag="VGB93_11595"
     CDS             210..1046
                     /gene="murI"
                     /locus_tag="VGB93_11595"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0146762.1"
                     /translation="MSKPPHVLVFDSGLGGLTVLAEGMRLRPDVSCLFAADDAGFPYG
                     RLAEPALIARVGTLMAHLVSDYAPDAVIIACNTAATAVLPHLRASYPQIPFIGTVPAV
                     KPAAAQSRSKLISVLATPGTVARDYTHDLVRTYAAHCDVTLVGSTQLASLAEACMKGE
                     DVTDAAIVAEIAPSFVEKDGQRTDCIVLACTHYPLLLKQFERLAPWPVAWIDPAEAIA
                     RRLDHVLREKLGGAESDAGNWPPHAALFTSGAAPSPALAKALHERGIGRILENPIPMA
                     IA"
     gene            complement(1043..2008)
                     /locus_tag="VGB93_11600"
     CDS             complement(1043..2008)
                     /locus_tag="VGB93_11600"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0146763.1"
                     /translation="MVDFASSRFVVAVDPQTPPAGFDGAVVAIGNFDGVHRGHIAVLK
                     RAEDIAAQLGRPCAVLTFEPHPADFFFRTDTIFRLTPQETKAVALARAGIDGMIALTF
                     DADLAAMPAEDFVRDILVRRLHVGGVVAGYDFHFGKGRAGTPAFLAEAGRRYGFAVEI
                     VERIAADSSGSIVPASSTETRKALEAGDVERAKVLLGHPYFVIADVVHGEKRGRDLGF
                     PTANLRLHHSVRLRHGIYAVRARIDDIWFGGVASFGTRPTFDNGAPLLEVYVFDFSGD
                     LYGKTLEIAFVHFLRDELKFDSIETLVAQMRRDEAEARKILAADA"
     gene            2275..>2359
                     /locus_tag="VGB93_11605"
     CDS             2275..>2359
                     /locus_tag="VGB93_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604011.1"
                     /note="chemotaxis regulator that, when phosphorylated,
                     interacts with the flagellar motor causing the flagella to
                     spin clockwise which causes the cell to tumble; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEY0146764.1"
                     /translation="MIVEPSLPILIVDDYKTMVRIIRNLLGQ"
BASE COUNT          502 a          733 c          682 g          442 t
ORIGIN      
        1 ttcgatgaaa gattgttcgt ccgcagcttt ttcgctcgcc atgccccctc cgcgagagtt
       61 caacgcggat gccgccgaaa aagcctcaaa tggaatcaca gatcacgcga actgttacat
      121 ccgcgcctct cgccctcgaa atgggtcgat cttggagccc gaaaaggcta aggaccgggt
      181 ataattgagc gcccctcgtc tggagactta tgtcaaaacc gccgcacgtt ctcgtcttcg
      241 attctggatt gggtggcttg accgttctgg cggaagggat gcgcctgcgg ccggacgtga
      301 gctgcctttt cgcggcggac gatgcaggtt ttccctatgg ccggctcgcc gaacccgcgt
      361 tgatcgcgcg cgtcggcacg ctgatggccc atctcgtttc ggattacgcg cccgacgcgg
      421 tcatcatcgc gtgcaatacc gcggcgaccg cggtcttgcc gcatctgcgc gcctcttatc
      481 cgcaaattcc cttcatcggc acggtcccgg cggtgaagcc ggcggcggca caatcgcgct
      541 cgaagcttat ttccgtgctc gccacgccag gcactgtcgc acgggactat acccacgacc
      601 tggtgcgtac ctatgccgcc cattgcgacg tgacgctcgt tggctcgacg caactcgcca
      661 gcctcgccga ggcttgcatg aagggcgaag acgtcaccga cgctgcgatt gttgcagaaa
      721 tcgcgcccag ctttgtcgaa aaagacggcc agcgcaccga ttgcatcgtg ctcgcctgca
      781 cgcattatcc actgctcctc aagcaattcg agcggctcgc gccgtggcct gtcgcctgga
      841 tcgaccccgc cgaggccatc gcccgccggc tcgaccatgt gttgcgggag aagctcggcg
      901 gagcagagtc cgacgcggga aattggccgc cacacgcggc tttattcacc agcggcgccg
      961 cgccaagccc ggcgcttgcc aaagcgcttc atgagcgggg gatcggcaga atactcgaaa
     1021 acccgatccc gatggcgata gcttaggcat cggccgcgag aatttttcgc gcctcggcct
     1081 cgtcgcgacg catctgcgca acgagagttt cgatcgagtc gaatttcaac tcatcgcgca
     1141 gaaaatggac gaaggcgatt tcgagcgtct tgccgtagag atcgccggaa aaatcgaaga
     1201 cgtagacttc gagcaagggc gcaccattgt cgaacgtcgg acgggtgccg aaactcgcga
     1261 cgccgccgaa ccagatgtca tcgatgcgcg ctcgcacggc ataaattccg tgccgcaggc
     1321 gcacagaatg atgaagccgc aaattggcgg tcggaaagcc gagatctcgg ccacgctttt
     1381 cgccgtgcac gacatcagcg atgacgaaat agggatggcc gagcagaact ttcgcccgct
     1441 ccacatcgcc ggcttcgagc gcttttcgcg tttcggtaga cgaggctggc acaattgaac
     1501 cactcgaatc ggcggcgatc cgctcgacga tctcgaccgc aaaaccgtag cgtcggccag
     1561 cttcggcgag aaaggccggc gtgcccgccc tgcccttgcc aaagtgaaaa tcatagccgg
     1621 caaccacacc gccgacgtgc aggcgtcgga cgagaatatc gcgaacgaaa tcttctgccg
     1681 gcatggcggc gaggtcggca tcgaaggtca gcgcaatcat cccgtcgatc ccggctcgcg
     1741 ccagcgcaac ggccttcgtt tcctgcgggg taagccggaa aattgtatcg gttctaaaaa
     1801 agaaatccgc cggatgcggc tcgaaagtca aaaccgcgca agggcgtccg agctgagccg
     1861 cgatgtcttc ggcacgcttc aagacagcga tatggccgcg atgcacgcca tcgaaattcc
     1921 cgatagcaac cacagcccca tcaaaaccag cggggggagt ctgaggatcg accgcgacga
     1981 caaaacgcga tgacgcgaaa tcgaccatta gcaaccagag aggtggagcg tatcgccaag
     2041 cggcgcgaac ctgccccgcg aagcgacatc cgtcaagctg cggcattttc gccagcgtgg
     2101 acaagaagac gttaccttgt tgccccgcaa tttgaccgat ccgagagttt tccggatcgc
     2161 aattaactga attgcaagcg cccatccgtt taattgcagc ggcgccgatg aagttgattt
     2221 ggcggcgcgg catagctttt tattaaccga ccgttttatc ggccggagga cttaatgatt
     2281 gtggaaccgt cacttccgat ccttatcgtc gatgactaca agacgatggt tcggatcatc
     2341 cgtaacctgc tcggccaga
//
LOCUS       DASWOG010000567         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_123607,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000567 DASWOG010000000
VERSION     DASWOG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123607"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1914)
                     /gene="guaA"
                     /locus_tag="VGB93_11610"
     CDS             complement(313..1914)
                     /gene="guaA"
                     /locus_tag="VGB93_11610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0146765.1"
                     /translation="MIKPEDPLHHDIIAHHDKVLIVDFGSQVTQLIARRVREAGVYCE
                     IAPYKKAEAAFADLKPKAVILSGGPASVLEEGSPRLPQAIFEAGVPILGICYGEQLMA
                     AQLGGEVQGGHHGEYGRAEVEAIAESPLLEGVWEQGRHTVWMSHGDRVTKLPPGFQSI
                     GHSPNAPVCVIADEARRFYGLQFHPEVHHTPEGPQLLGNFLFKIAGFRRDWTMRAFRA
                     EAIKVIRAQVGQGRVLCALSGGVDSSVAALLIHEAIGDRLTCVFVDHGLLREGEAEQV
                     VSLFRGHYNIPLVHVDASETFLKALNGVQEPEEKRKIIGKLFIDVFDEEAKKIAADGR
                     GAPDFLAQGTLYPDVIESVSFQGGPSVTIKSHHNVGGLPARMKMKLVEPLRELFKDEV
                     RALGRELGLPDVFVGRHPFPGPGLAIRLPGGVTAEKLAILRKADAIYLDEIRKAGLYD
                     KIWQAFVVLLPVKSVGVMGDGRTYDFVCALRAVTSTDGMTADFFHYDMNFLGRAATRI
                     INEVRGINRVVYDVTSKPPGTIEWE"
BASE COUNT          413 a          656 c          640 g          412 t
ORIGIN      
        1 cagggtttgg gcgatcgcgg cctgaaaagc ttccgcttcg gcaaagatat cgacggcggg
       61 gatgattgag agcttgatga cacactcgcc cagcttgacc gcaacatgcg cctgcggtcg
      121 cgtggaactt aacgcgtccc acaggacgcc tcaagcgccg atgggtttat ctattataaa
      181 tcagttagtt gcttactcac gacttgatga gatacacttg tggtatctga tcgcgtcaag
      241 ataaatgcgt gcacatatct tttgttagcc gctaaacgcg tcatcaaatc gaattaaagg
      301 agctagtgtc cttcactccc actcaatcgt ccccggcggc ttgctcgtca cgtcatacac
      361 aacgcgattg atgccgcgca cttcgttgat gatgcgcgtc gcggcgcggc cgaggaaatt
      421 catgtcgtag tggaaaaagt ccgccgtcat gccgtcggtg gatgtcacgg cgcgcaaggc
      481 gcagacgaaa tcataggtac gtccgtcgcc catgacgccg acacttttca ccggcagcag
      541 cacgacgaag gcctgccaga tcttgtcgta gagcccggcc ttgcggattt cgtcgagata
      601 gatggcgtcg gccttgcgca agatcgcgag tttttccgcc gtcacgccgc ccggcaggcg
      661 gatggcgagc cccggccccg ggaacggatg ccggccgacg aaaacatccg gcaggccgag
      721 ttcgcggccg agcgcacgca cctcgtcctt gaagagttcg cgcagcggct cgacgagctt
      781 catcttcatc cgcgccggca ggccgccgac attgtgatgc gacttgatcg tgacgctcgg
      841 gccgccctgg aacgagacgc tttcgatcac gtccggatag agcgtgccct gcgcgagaaa
      901 gtccggcgcg ccgcggccat cggcggcgat cttcttcgcc tcttcgtcga agacatcgat
      961 gaacagcttg ccgataatct tgcgcttctc ttccggctcc tgcacgccat tgagcgcttt
     1021 caggaacgtc tcgctcgcat cgacatgcac gagcggaatg ttgtaatggc cgcgaaacag
     1081 cgagacgacc tgctccgcct cgccttcacg cagcagacca tgatcgacga agacgcaggt
     1141 gagccggtcg ccgatcgcct catggatcag cagcgccgcg acggaggaat cgacgcctcc
     1201 ggatagcgcg cagagtacgc ggccctgccc gacctgcgcg cggattacct tgatcgcctc
     1261 ggcgcggaag gcgcgcatgg tccagtcgcg gcgaaacccg gcaatcttga agagaaaatt
     1321 ccccaagagc tgcggcccct cgggtgtgtg atgcacctcg ggatggaatt gcagtccgta
     1381 gaagcggcgc gcttcgtcgg cgatgacgca gaccggcgcg ttgggcgaat ggccgatgct
     1441 ttgaaaaccc ggcggcagtt tggtcacgcg gtcgccgtgg ctcatccaga ccgtatggcg
     1501 gccctgctcc cagacgcctt cgagcagcgg cgattccgcg atggcctcga cttcggcgcg
     1561 gccatattcg ccgtgatggc cgccttgtac ctcgccacca agctgcgccg ccatgagctg
     1621 ttcgccgtag cagatgccga ggatcggcac gccggcctcg aagatcgcct gcggcaagcg
     1681 gggcgagcct tcctccagca ccgaggccgg gccgcccgac aggatcaccg ccttgggctt
     1741 caaatccgcg aacgcagcct cggccttctt atacggcgcg atctcgcaat agacgccggc
     1801 ctcgcgcacc cggcgggcga tcaattgcgt cacctgcgag ccgaaatcga cgatcagaac
     1861 cttgtcgtga tgcgcaatta tgtcgtggtg gagagggtct tcgggcttga tcaagcgact
     1921 ggctcctgcc gcaaaaattc gaattaagag attgcacccc gcggcgcaag gaagtgcgga
     1981 gctttacctg aatgctttcc aacgcatttg aactttccgg ttgaaggccg ggcaatgcct
     2041 ctgcgctagt ctgcatcatg cgtagagttg gtattgtcga ggctaagatg gatcgcattg
     2101 tccgggttta cgacccgcag c
//
LOCUS       DASWOG010000568         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_86608,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000568 DASWOG010000000
VERSION     DASWOG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86608"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1296)
                     /locus_tag="VGB93_11615"
     CDS             complement(70..1296)
                     /locus_tag="VGB93_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146766.1"
                     /translation="MGSRHANILGKSFSYLELYENQDLPRWDEICAYCQKIGFDYVTL
                     PPPFAPGQKGDAFLTGDIEFAHPTLAGLAGGARSAKTVDVIETLSQTCRAHGLGIVFD
                     IVIDRADARGMLVSNNRELFDDALSESLNPRSLRATAGAATARFDNGGQLERLIALWA
                     GCLGALLTVGASGFRFLNPGAVATPPWRLLHTTLRDAFSEMISLANTPGMSWQAIGGL
                     RDCEFDGAFCSLPWWDLRSAWFADEINLLHRIGCALAAPRRFGPDCSEGPAADAARER
                     QQLHIAAAIADGLFIPLDFGPSQIGGGAQTRELCATIEQFARIKRRGTQGEMRALCSA
                     GGAITALVHRNLSNTVESETAVVTLINRDLSDSEEIDVPLESLDSTSGVALGNPSRLN
                     GSGDPCPPWSRVRCAW"
BASE COUNT          427 a          579 c          581 g          378 t
ORIGIN      
        1 gaatggcgtg ttcaagcact tcgcgcggat cctcggcagg cgacttgatt ggagtgagtg
       61 gctcaagctt taccacgcgc acctcacccg gctccaggga ggacaaggat cgccgctgcc
      121 gttgaggcgt gacggattgc caagtgccac gccagacgtc gaatcgaggg attcgagcgg
      181 aacatcgatt tcctcgctgt ctgacaaatc gcgattaatc agcgtaacca ctgctgtttc
      241 cgactcgacc gtattagata gattgcgatg cacgagcgcg gtgatggcgc cgcctgcact
      301 gcacaacgcc cgcatctcgc cttgcgttcc acgccgcttg attcgtgcaa attgctcgat
      361 cgttgcgcaa agctctcgtg tctgcgcgcc gccgccaatt tgcgaagggc cgaaatccaa
      421 cgggatgaag agcccgtcgg caattgctgc ggcaatgtgc aattgttggc gctcccgggc
      481 cgcgtctgcg gccggccctt cggagcaatc cggaccgaag cgacgcggag cggccaatgc
      541 gcaacctatc cgatgcagca ggtttatttc gtctgcgaac catgccgagc gcaaatccca
      601 ccacggcaac gaacagaaag cgccatcgaa ttcgcagtcg cgcagaccgc cgatcgcttg
      661 ccacgacata cccggcgtgt tggcaaggga gatcatctcc gagaaggcgt cgcgcagtgt
      721 cgtatgaagg agacgccagg ggggcgtcgc gacggcacct gggttcagga agcgaaaccc
      781 gctggcaccg acggtcagca aagcgcccag acagccagcc caaagcgcaa ttaatcgctc
      841 cagctgcccg ccattgtcaa agcgcgcagt tgcagccccg gccgttgcgc ggagcgagcg
      901 tggattgaga gattccgaga gagcatcgtc gaagagttcg cgattgttcg agacgagcat
      961 gcctctcgcg tcggcgcgat cgatgacgat gtcgaagaca ataccgaggc cgtgggcgcg
     1021 gcaggtctgg cttaacgttt cgatgacatc gacggtcttg gcgctgcggg cgcccccggc
     1081 gagcccggcg agcgtcggat gcgcaaattc tatatcaccc gttagaaaag cgtcaccttt
     1141 ctgacccgga gcgaagggcg gcggcaaagt gacataatcg aagccgattt tttgacaata
     1201 cgcgcaaatc tcgtcccagc gcggcaggtc ttggttttcg tataattcaa ggtaggaaaa
     1261 gctcttgccg aggatatttg catgtcggct gcccaacgcg gactatccca ttcgccgtaa
     1321 aggtaagttc cgcttaaact gcgcaggcaa cggcgaagtt ccaaaacaat aattcattat
     1381 gaagaactac ctgctaagcg acgggtctac acgcgcggaa ctgatcgccg acccaagttt
     1441 gattctctga tggtcgcgat cgatatcagc gacgtccatt ttcaatagca attccactcc
     1501 atttcgccga ccgccccgaa cattttccag agcgtcaagg atcgtctcga gaacggcgat
     1561 ctcctccaaa agtcttgtcg atcatgcgac agccgctcgg acgttccgcc gaatgatttg
     1621 tggcggttgt ccgagcgcac gcaagaatgc ccgccgcata cgttcacgat cccgctgcac
     1681 cgctttaacg cccaataaaa gccggcttcg gccgagagtc cgcaggacgc agacttcgga
     1741 tctggctgca gtttgcaggt tgcagacgac tgagatgcgc cgggcaggaa ccttatttgc
     1801 tttcgtcggc acgctcctcc ggcggaactc ggcccgccgg acgaatggca gtaattggcc
     1861 gaagaacgaa ccgcttcgcg gacggcgctt gggactgagg gggatgggcc ctgacgggca
     1921 ttgagcttgc ctctgagagc gccggtacgt gatgtgacca agata
//
LOCUS       DASWOG010000569         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_288469,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000569 DASWOG010000000
VERSION     DASWOG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288469"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VGB93_11620"
     CDS             <1..792
                     /locus_tag="VGB93_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0146767.1"
                     /translation="SDEIAKAGATPLGVAKDGRLLGVIHLKDIIKGGIKERFQELRRM
                     GIRTVMITGDNPLTAAAIAAEAGVDDFLAQATPEMKLKLIRSEQAQGKLVAMCGDGTN
                     DAPALAQADVGVAMNTGTMAAREAGNMVDLDSDPTKLIEIVEIGKQLLMTRGALTTFS
                     IANDVAKYFAIIPAMFLAFYPQLQALNVMHLHTPQSAILSAIIFNALIIIALVPLALK
                     GVTYRPLGAANILRRNLLVYGLGGIIVPFIGIKLIDMAVSAIGLA"
     gene            803..1423
                     /locus_tag="VGB93_11625"
     CDS             803..1423
                     /locus_tag="VGB93_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEY0146768.1"
                     /translation="MLKQIRPAILLIVLMTIITGLAYPLGMTGIAQVLFPHQANGSLI
                     EKDGKVIGSTLIGQNFVSDKYIHGRLSATTEPDAKDPTKSDPVPYSADNSTGSNLGPT
                     SKALIQRVQADAARLHKENPSVPVPVDLVTTSASGLDPDITPAAAYFQVSRVAKARNV
                     TEDALKTLVAAHIEGRLFGIIGEPHVNVLDLNLALDRAEKVVSGGL"
     gene            1439..>3209
                     /locus_tag="VGB93_11630"
     CDS             1439..>3209
                     /locus_tag="VGB93_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0146769.1"
                     /translation="MDISSGDKRPSPEALLREAENGTRGRLKIFLGAAPGVGKTYEML
                     LMAQAKRREGVDVVVGVVETHGRAETEALVAGLEVIPRRNVVYKGRVLTEMDIDAILA
                     RHPKLVLVDELAHTNAPESRHPKRYLDVEELLAAGIDVYTTVNIQHIDSLNDVVARIT
                     HTRVRETVPDSIIDHADDIEVIDLTPDDLLARLREGKVYVGDRAARAIENIFSRGNLT
                     ALRELALRRTAQRVDAQMVDYMQSHAIDGPWPAGERVLVAIDGGGGSAALVRRARRLA
                     EYLRAPWTALHVETSRELRCDEAERKIVAETLQLATRLGGTAVTLPGEDAATTILDYA
                     HTHNVTHILVARTGRASWLDILRGSTLNKLIQNAGGINIHIATADSAVGKTRHFAFVA
                     PDSPRDWTVYLKSLVFVAGAGAVAFLLRETLGVSSVALVFLIGVLASAVCYGLFPSLV
                     AALASALAYNFFFLPPLYTFTIADPENVVALTTFAIVAVIASNLAARLRTQAIAARAR
                     ARITEELYLFSRKLAGAASLDDIIWASVYEIALLLKVNAVLLLPRNGKLEPMGGYPPE
                     DRLDEADFAAAQWSFERNHAAGRG"
BASE COUNT          622 a         1013 c          930 g          644 t
ORIGIN      
        1 tctgacgaaa tcgccaaggc gggcgcgaca ccgctcggcg ttgccaagga cgggcgcctc
       61 ctcggcgtca tccacctcaa ggatattatc aaaggcggca tcaaggagcg cttccaggaa
      121 ttgcgccgca tgggtatccg cacggtgatg atcaccggtg acaatccgct cactgcggcg
      181 gcgatcgcgg ccgaagcggg ggtcgatgac ttcctcgccc aggcgacgcc ggaaatgaaa
      241 ctgaagctca tccgctctga gcaggcgcaa ggcaaactcg tcgcaatgtg cggcgacggc
      301 accaacgacg ccccggcatt ggcgcaagcc gatgtcggtg tggcgatgaa cactggcacg
      361 atggcggcgc gcgaagccgg caacatggtc gatctcgaca gcgatccgac caagctgatt
      421 gagatcgtcg agatcggcaa acaattactg atgacgcgcg gcgcgttgac gaccttctcg
      481 atcgccaacg acgtggcgaa atatttcgcc atcatcccgg cgatgttcct cgccttctat
      541 ccgcaactcc aggcgctgaa cgtcatgcac ctgcacacgc cgcagagcgc gattctctcg
      601 gccattatct ttaatgcgct gatcattatc gcactggtgc ctctggcgct caaaggcgtc
      661 acgtaccgcc cgctcggcgc ggccaacatt ctgcgccgta acctgctcgt ctacgggctc
      721 ggcggtatca tcgtgccgtt catcggcatc aagctcattg acatggcagt ttctgccatc
      781 ggtcttgcgt gaggattttc caatgttgaa acaaatccgc cctgccatcc tgctgattgt
      841 cttgatgaca atcatcactg gcctcgccta cccgctcggc atgaccggca tcgcccaggt
      901 tctcttcccg catcaggcaa atggcagcct gatcgagaag gatggcaaag tgatcggttc
      961 gacgctgatc ggccagaact tcgtcagcga caaatatatc cacggccgcc tctctgcgac
     1021 cacggaaccc gatgccaagg atccgacgaa atcagatccc gtgccctatt ccgccgacaa
     1081 ttccacgggc tcgaacctgg gaccgacttc gaaagcgctt atccagcgcg ttcaggccga
     1141 tgccgcccgg ctgcataagg aaaacccctc cgtgcccgtg ccggtggatc tcgtcacgac
     1201 ttcggccagc ggtctcgacc cggacatcac gcccgcagcg gcctatttcc aggtgtcgcg
     1261 cgtcgccaaa gcccgcaacg tcacagagga cgcgctcaag acacttgtcg cagcacatat
     1321 cgagggacgg ctcttcggca tcattggcga gccgcatgtt aatgttctcg acctgaacct
     1381 cgccctcgac cgcgctgaaa aagtcgtgag cggcgggtta tgagataaga tcggtgaaat
     1441 ggatatttca tccggcgaca aacggccgtc tcccgaggcc ctgctccgcg aggccgaaaa
     1501 cggcacgcgc ggacggctca agatttttct tggtgcggcc ccgggagtcg gcaaaactta
     1561 tgaaatgctg ctgatggccc aggccaagcg gcgcgagggt gtcgatgtgg tcgtgggcgt
     1621 cgtcgaaacc cacggccgcg ccgaaacaga agcactcgtc gccggcctcg aagtcattcc
     1681 ccgccgcaac gtggtctata agggccgagt cctgacggag atggatatcg acgctattct
     1741 ggcgcgccat cccaagctcg ttctggtcga cgaactcgct catacgaacg cgcccgaaag
     1801 ccgtcacccg aaacgctacc tcgatgtcga ggaattgctg gccgccggga tcgatgttta
     1861 taccaccgtt aatatccagc atatcgacag tctcaacgat gtcgtcgcgc gcatcacgca
     1921 tacccgcgtg cgcgagacag ttcctgactc catcatcgat cacgccgacg acatcgaagt
     1981 catcgatctc acgcccgacg atcttctcgc cagactgcgc gagggtaaag tctatgtcgg
     2041 cgatcgggct gcgcgcgcga tcgagaatat tttttcgcgc ggcaatctca cagctttacg
     2101 tgagcttgcc ttacgccgaa ccgcgcagcg ggtcgatgcg caaatggtcg attacatgca
     2161 atcacatgcg atcgatggac cgtggcctgc tggcgagcgc gtgctggttg cgattgatgg
     2221 tggtggtggc agcgcggctt tggtgcgccg ggcgcggcgc cttgccgaat atctgcgcgc
     2281 gccctggact gcgctgcacg tcgagacatc acgcgaactg cgttgcgacg aagcagaacg
     2341 caagatcgta gccgaaactt tgcagcttgc aacgcggctc ggcggaaccg ccgtcaccct
     2401 tcccggcgag gatgccgcaa cgacgatcct cgactatgcc catacgcaca atgtcacgca
     2461 catccttgtt gcccggaccg gccgtgcaag ctggctcgac attctgcgcg gctcgactct
     2521 caataaattg atccagaacg ccggcggcat caacattcac atcgcaacag cggattccgc
     2581 cgtcggaaaa acgcgacatt ttgcgtttgt ggcgcccgat tctccgcgcg attggaccgt
     2641 ctatcttaag agcctcgtct tcgtcgccgg cgcgggcgcc gtcgcttttc tgttgcgcga
     2701 gacgctcggc gtttcgagcg tcgcgctggt tttcctcatc ggcgtgttgg caagcgccgt
     2761 ctgctacgga ctgttcccgt cgcttgtcgc cgcgttggcc agtgcgctgg cttacaattt
     2821 ctttttcctg ccaccgcttt acacgtttac gattgccgat cccgaaaatg tcgtcgcgct
     2881 gacgactttc gcgattgttg cggtgatcgc cagcaatctc gccgcgcgtt tgcggacgca
     2941 ggcgatcgcg gcgcgggcgc gggcccgcat taccgaagag ctttatcttt tcagccgcaa
     3001 gctggccggg gctgcgtcgc tcgacgatat catctgggcg agtgtctacg agatcgcgct
     3061 gcttttgaaa gtgaatgcgg ttttgctgtt gccgcgcaac ggcaagctcg agccgatggg
     3121 tggctatccg cccgaggatc gcctagacga agccgatttc gccgcggcgc aatggtcgtt
     3181 cgagcggaat cacgctgccg gccgcggtg
//
LOCUS       DASWOG010000570         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_326043,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000570 DASWOG010000000
VERSION     DASWOG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326043"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1524
                     /locus_tag="VGB93_11635"
     CDS             382..1524
                     /locus_tag="VGB93_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY0146770.1"
                     /translation="MTDRIQEFLRDRRQKGLEDGPCLVLDLDVVRDNYTAFNKALPDT
                     RIFYAVKANPDAQVLSLLASLGSCFDTASVVEIEQAFAAGATPDRISFGNTIKKERDI
                     ARAYALGVRLFAVDCEAEVEKIARAAPGAKVFCRILCNGDGAEWPLSRKFGCVPEMAA
                     RVLEHAHRLGLVAYGLSFHVGSQQRNPKSWDAALQASAAIFRDLAERGISLQMVNLGG
                     GFPTKYLRDVPAVKAYGQAIFRALRKHFGNRIPETIIEPGRGMVGNAGMIEAEVVLIS
                     RKSDDEKMRWVYLDIGKFNGLAETMDEMIRYPIRTAFDEDAMEPCIIAGPSCDSVDVL
                     YEKEPYPLPLSLEIGAKVLIEGTGAYTTTYSAVGFNGFPPLKSYVI"
     gene            complement(1635..2336)
                     /locus_tag="VGB93_11640"
     CDS             complement(1635..2336)
                     /locus_tag="VGB93_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146771.1"
                     /translation="MTARTFVFVVCSLSGHAGKTTAARLLGDYFLASRRTFRGFDTDA
                     HEPDFARRFPDEAIVADISTIQGQMALIDPLLENDSIPKIIDLWHRSFEAFFTLIDHT
                     GFIAEARSRGVEPILLYVDDGAPRAVDVAARIGARYPGLQVMIVQNQMVSPEAADEPG
                     GPALRSFKIGRIDPLLQATIDKPGFSLSQFMRAPPSDMSIVLRAGLRDWVWRVFSQFK
                     SFELRMTLSESEHLG"
     gene            complement(2383..3126)
                     /locus_tag="VGB93_11645"
     CDS             complement(2383..3126)
                     /locus_tag="VGB93_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146772.1"
                     /translation="MGHNQMKPTLIMVGADKGGVGKTTVARSLLDFLASNNVIVRAFD
                     TETPRGTLQRFHPEITQVVDINQTPDQMRILDTLNTSQVKVTLIDTRAGALASSLAAL
                     RDVGFLDAAKAGEFNFVLFHILGPSIASLSEINETAPYVADAHYFLVKNYVNDSTFFD
                     WDPATLQTYFGKVKNANDVTIPKLNAMAYEQVELAGTPLSTFVADKTASGEPANHSFT
                     LRGYVRTWQNKNADEFNRIRLLDLVNGRG"
BASE COUNT          684 a         1005 c         1049 g          667 t
ORIGIN      
        1 cagccggagc gaaccggtga accccgccaa acgggggaca cgacttaaag caacgacgga
       61 ccgggctttt tcgtctctgc cagcttccaa aactggctta ccgaagaggc tagtctttct
      121 tgccgggcgt gcggagcggg actccatccc atctccaaac gaaggccatg aggtttcata
      181 acggcaattt gccgtgcgaa ccgcattgcc gacggcgcag catctggttt ccgcaacgcg
      241 agtctcctcg cggggtggcg gccagcgatg acgggagcgg tgagcggtgg cgtctccaca
      301 atgcgccagg atccaccttc gtcatcatcc caagcttgtt tcgcttctgc gtggggaacc
      361 gcgcttcgag gggatgccgc gatgaccgat cgtatccaag aattcctgcg tgatcgacgc
      421 cagaaaggcc tcgaggatgg cccgtgcctt gtcctcgacc tcgacgtcgt gcgcgacaat
      481 tacaccgcct tcaacaaggc actgcccgac acgcgcatct tctacgccgt gaaggcgaat
      541 cccgacgcgc aggtgctctc gctactcgcc agccttggct cgtgctttga cacggcctcg
      601 gtcgtcgaga tcgagcaagc gtttgccgct ggcgcgacgc ctgatcgcat cagcttcggc
      661 aacacgatca agaaggagcg tgacatcgcc cgcgcttatg cgctcggcgt gcgtcttttt
      721 gcggtcgatt gcgaagccga agtcgagaag atcgcccgcg ccgcgcccgg tgcgaaggtg
      781 ttctgccgca tcctctgcaa cggcgacggt gccgaatggc cgctatcgcg caagtttggc
      841 tgcgtgcccg agatggcagc gcgggtgctg gagcacgcac atcggctcgg gcttgtcgcc
      901 tacggcctct cgttccatgt cggctcgcag caacgcaacc cgaagagctg ggacgcggcg
      961 cttcaggctt cggcggcgat cttccgtgat ctcgccgaac gcggcatctc gctacagatg
     1021 gtcaacctcg gcggcggctt cccaacgaag tatttgaggg acgttcccgc ggtgaaagcc
     1081 tatgggcagg cgatcttccg ggctctgcgc aagcatttcg gcaaccgtat tccggagacg
     1141 atcatcgagc cgggccgcgg tatggtcggc aacgccggca tgatcgaggc cgaggtcgtg
     1201 ctgatctcga ggaagtcgga cgatgagaaa atgcgctggg tctatctcga cattggcaag
     1261 ttcaacggac ttgccgagac gatggacgaa atgatccgct atccgatccg taccgccttc
     1321 gacgaagatg cgatggagcc ttgcatcatc gccgggccgt cgtgcgactc ggttgatgta
     1381 ctctacgaga aggagcccta cccgctcccg ctcagcctgg agatcggcgc caaagtgctg
     1441 atcgaaggaa caggcgctta tacgacgacc tattcggcag tcggcttcaa cggcttcccg
     1501 ccgctgaagt cctacgtcat ctagagcatc ggacggaaaa gcgcgaagcg gttttcggac
     1561 aaatccgatg cgatgaaaga taagagacgt aacttgggcg gggggaaatc tccgcccttt
     1621 ttattgaaat tccttcagcc cagatgttcg ctctcgctga gcgtcatgcg cagctcaaaa
     1681 ctcttgaatt gcgagaacac gcgccacacc caatcgcgca agcccgcgcg tagcacaatc
     1741 gacatgtcgg agggcggcgc gcgcatgaat tgcgaaagcg aaaagccggg cttgtcgatc
     1801 gtggcctgaa gcaggggatc gatgcggccg attttgaaac tgcgcagggc cggtccgcct
     1861 ggctcgtccg cagcctcggg actcaccatc tgattctgga ctatcatcac ctgcaggccg
     1921 ggatagcgcg ccccgatccg cgccgcgacg tcgaccgcac gtggcgcgcc atcatcgaca
     1981 tacagcagga tcggctcgac gccgcgcgac cgcgcctcgg cgataaaacc ggtatgatcg
     2041 atgagcgtga aaaaggcctc gaacgaacgg tgccagagat cgatgatttt agggattgaa
     2101 tcgttttcca gcagcggatc gatcaaagcc atctggccct ggatcgtgct gatatccgcg
     2161 acaatggcct cgtcgggaaa gcgacgggca aaatccggct cgtgcgcgtc ggtatcgaag
     2221 ccacgaaatg tacgccggct ggcaagaaag taatcgccga ggaggcgcgc cgcagtcgtc
     2281 ttaccggcgt ggccggacag cgagcaaacg acaaaaacga aggtcctcgc ggtcatggga
     2341 tgatgaccgc ggaagcgagg cgcagtgtat taatggcgca ggttagccgc ggccattcac
     2401 gagatcgagc aagcggatac ggttgaactc gtccgcgttc ttgttttgcc atgtacgcac
     2461 gtagccacgc agagtaaaag aatggttcgc cggctcgccg gacgcagtct tgtcggcgac
     2521 gaacgtcgat aggggcgtgc cggcgagttc gacctgctca tacgccatcg cgttgagctt
     2581 cgggatcgtc acgtcgttcg cgtttttcac cttgccgaaa taggtctgca acgtcgccgg
     2641 gtcccagtcg aagaacgtcg agtcgttgac gtaattcttg acgaggaagt aatgcgcatc
     2701 cgcgacatag ggggcggttt cgttgatttc cgacagcgat gcgatcgacg gtccgaggat
     2761 gtggaagaga acgaaattga actcgccagc cttcgccgca tcgagaaagc cgacatcgcg
     2821 gagcgcggcg agacttgagg ccaaagcgcc ggcgcgggta tcgatcagcg ttactttcac
     2881 ctgcgaagtg ttcagcgtat cgagaatacg catctgatcc ggcgtctgat tgatgtcgac
     2941 gacctgcgtg atctcgggat gaaaccgctg gagcgtgccg cgcggcgtct cggtgtcgaa
     3001 agcgcggacg atgacgttat tgcttgcgag aaaatcaagg agcgagcgcg ctacagttgt
     3061 cttgccgacc ccgcctttgt cggcgccgac cataatcagc gttggcttca tctggttgtg
     3121 ccccatcccc ggcgctcgcg cggaatcttg cgcctctgtt gcggtttccg acggctttgg
     3181 tcgctgcgtt cgggccaccc gcctcaaaga tttaattttg cgagccatac gccgatatac
     3241 agcactttta ccggtgcgcc aatcagagcg gaattttagt taatgccgtt gcatccgcag
     3301 cttagcgcgt gacgcgggga aattcctact tgtcttcgcg ggccgtggcg aaggccccgc
     3361 cgttaggcgc tttggcgatc aacgcgtcga tacggtcgcg ctcgc
//
LOCUS       DASWOG010000571         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_326046,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000571 DASWOG010000000
VERSION     DASWOG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326046"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VGB93_11650"
     CDS             complement(<1..1829)
                     /locus_tag="VGB93_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146773.1"
                     /translation="MATSNKAQDPAAAALSAIEEALNLSDSAPAAEEDKAPEISTPIF
                     PKASDPDKTLKIRKRYPEGPALGAPLPASPVDLDRAPPRLPEVEDHPLFAPRRPDNNV
                     DPGQGDFNANRPNLQPAATPANDDRHSVGAILKALNRRPSPAPLIVAGVLSVVWLALA
                     GFFFMAHRADYFAHISVSRPETLIFALLTVGPVIFFMVTGLLARRAQEMRLTARSMAE
                     IAVRLSAPETIASDQMVTLSQAIRREIVSMGDGIERALARAGELETLVRSEVSNLERS
                     YSENERRIRSLVDELQTERESMMANSERMRSAVGGAQDSFAQELDTAAVRLSENLNNA
                     GNRITTTLGSTAEEIRFSIARVGEDLLGTLATHGEQIIGRLEQTRASVDETLTRANGA
                     LAETFNQRLGDVDAHLHETSEAFANHIVQRLDDASGRVATLLGGTTSSFEEISQNFAQ
                     RLEDVDAHLRTTGEAVVSSIVRELDEAAERAATALGGTTSAFDDISQGLSEKLADANA
                     QLRGSSENVVAEITQKLEEASARIAAVFEDSSSSFAAHSDKMHERIATTAQESFDHFA
                     YHAGTLNDRFAETAGDAISAIGMHSERINETLAARLSRFEDTIA"
BASE COUNT          356 a          570 c          583 g          370 t
ORIGIN      
        1 gcaatcgtat cttcaaagcg cgacagccgt gccgccagcg tttcattgat acgttcgcta
       61 tgcatgccga tggcgctgat cgcatcgccc gctgtctcag cgaagcgatc gttgagcgtt
      121 cctgcgtggt aggcgaaatg atcgaacgat tcctgcgctg tcgttgcaat gcgctcgtgc
      181 atcttgtcgg aatgcgccgc aaaggacgaa gacgaatctt caaagaccgc cgcaatccgg
      241 gcgctggctt cttccagctt ctgcgtgatc tcagcaacga cgttctcgct gctgccgcgc
      301 aattgcgcat tcgcatcggc gagcttctcg ctcaaaccct gtgagatatc gtcgaaagcc
      361 gacgtcgttc cgccgagtgc cgtcgccgca cgctctgcgg cttcatcgag ctcgcggacg
      421 atgctggaga caacggcttc gcccgtcgtg cgcaaatgcg cgtcgacatc ctcaagccgt
      481 tgcgcgaagt tctgggagat ctcctcaaag gaggacgttg ttccgccgag cagcgttgcg
      541 acacggccgc tggcgtcatc gagacgctga acgatatgat tggcgaatgc ctcgctcgtc
      601 tcatgcagat gcgcatcgac gtcacccaga cgctggttga aggtttctgc gagcgcgccg
      661 ttggcgcgtg tcagggtctc gtcaaccgac gcgcgggtct gctcaagccg tccgatgatc
      721 tgctcgccgt gcgtagcaag tgtgccgagc aagtcttcac cgacgcgcgc gatcgaaaag
      781 cgaatctctt ccgcagtcga accgagggtg gtcgtgatgc gattcccggc attgttgagg
      841 ttttcggaga gacgaaccgc tgcggtatcg agttcctgtg cgaaggaatc ctgcgcgccg
      901 cccacggcgg aacgcatccg ctcgctgttg gccatcatcg attcacgttc ggtctgcaat
      961 tcatcgacga gcgaacgaat gcggcgctcg ttttccgaat aggagcgctc gaggttggac
     1021 acttcggaac gaacaagggt ctcgagttcc ccagcgcgtg ccagcgcccg ctcgatgccg
     1081 tcgcccatcg agacgatctc gcggcgtatg gcttgcgaca gcgtcaccat ctgatcgctg
     1141 gcgatcgtct ccggcgccga aagccgcacg gcgatctcag ccatcgagcg cgcggtgagg
     1201 cgcatttcct gcgcgcggcg cgccagaagc cccgtaacca tgaagaatat gaccggaccg
     1261 acggtcagaa gcgcaaaaat caaagtttcc ggccgcgata ccgagatgtg cgcgaaataa
     1321 tcggcgcgat gcgccatgaa gaagaagccg gccaaagcca gccagacgac cgagagaacg
     1381 cccgcgacga tgagcggcgc tgggctcggc cgccgattca gcgctttcag aatcgcgccg
     1441 acagaatggc gatcgtcatt ggccggcgtg gcggcgggct gaagattggg acggttggcg
     1501 ttgaaatcgc cctgccccgg gtcgacgttg ttgtccggcc gccgcggcgc gaagagcggg
     1561 tggtcttcca cttccggcag tcgcggcggc gcgcgatcca gatcaacagg cgatgccggc
     1621 aatggcgcgc cgagagccgg tccttcagga tagcgtttcc tgattttgag tgtcttgtcg
     1681 gggtccgagg ccttgggaaa aatcggcgtc gaaatttccg gcgctttatc ttcttcggca
     1741 gccggcgcgc tgtcggagag gtttagggcc tcttcaattg ccgacagcgc tgcagccgcc
     1801 ggatcttgcg ccttattgga cgtcgccatt ttgacctctt acgcaaacga aatacgcacc
     1861 gaacaagaac gctacggca
//
LOCUS       DASWOG010000572         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_86634,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000572 DASWOG010000000
VERSION     DASWOG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86634"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGB93_11655"
     CDS             <1..276
                     /locus_tag="VGB93_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY0146774.1"
                     /translation="RAGTPAALMPQLPPGIAAARAKTLRQAGDAALQRHLTAQTGKIL
                     TVLTERGGTARAEDFTKVRVGNIPAGRLMNARIGRHDGKMLIAEALS"
     gene            369..1961
                     /gene="purH"
                     /locus_tag="VGB93_11660"
     CDS             369..1961
                     /gene="purH"
                     /locus_tag="VGB93_11660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0146775.1"
                     /translation="MSEKLHSIARAMISVSDKTGLVEFAQALAARGIEIVSTGGTHKA
                     LVAAGIAARDVSELTGFPEMMDGRVKTLHPKVHGGLLAIRENPEHEAAMLAHDIAPID
                     LLVVNLYPFESTVAKGADLDECIENIDIGGPAMIRAASKNFDAVNVIVDVADYPKLLV
                     ELEANAGKTSFGFRRALAQKAFACTAVYDAGISNWFAAQIGEKAPYYRAFGGLKLEAL
                     RYGENPHQAAAFYANEQPSFGLATAKQIQGKQLSYNNVGDTDAALELVSEFDPAKTAA
                     VAIIKHANPCGVAQAQSLDEAYRLALRSDPISAFGGIIALNQTLDAATAREIIKIFTE
                     VIIAPDATEEARAIIATKLNLRLLLTGGLPDPRRRELTFRSVNGGFLVQGRDNALVDD
                     MELQVVTKRAPTAAELADLKFAFRVAKHVKSNAIVYAKGGATVGIGAGQMSRVDSSRI
                     AAQKALEAAQTAGLPESLAKGAVVASDAFFPFSDGLLAAASAGATAVIQPGGSLRDGD
                     VIEAADAEGLAMVFTGARHFRH"
     gene            complement(1984..2937)
                     /locus_tag="VGB93_11665"
     CDS             complement(1984..2937)
                     /locus_tag="VGB93_11665"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY0146776.1"
                     /translation="MRTYLDFEKAVADLDTKVEELRTLVGDGENGVGSEDLTRLEIRA
                     EKALADLYASLTPWQKTQVARHPQRPHCADFVAGLVTDFTPLAGDRLFGEDAAIVGGF
                     ARFRGTSVCLIGQEKGNDTETRLKHNFGMARPEGYRKAVRLMELADRFSLPVISLVDT
                     AGAFPGVDAEERGQAEAIARSTDKSLSLGVPNVAVVIGEGGSGGAIAIAACNRVLMLE
                     HAIYTVASPEASASILWRDAARAQDAATNMKITAQDLLRFGLIDAIISEPIGGAHRDP
                     AAAIAATSNAIASALESFGNMSPDALRDARADKFLAMGRKT"
BASE COUNT          600 a          963 c          952 g          530 t
ORIGIN      
        1 cgagccggaa cgccggcggc actgatgccg cagctcccgc caggcattgc cgccgcgcgc
       61 gcgaaaacat tacggcaggc cggcgacgcg gcgctccagc gccatctcac ggcccagacc
      121 ggaaagatcc tgaccgttct gaccgagcgc ggcggcaccg cgcgggcgga ggatttcaca
      181 aaagtgcgcg tcggcaacat tccggccgga cggctcatga acgcccggat tggccgccac
      241 gatggcaaaa tgttgatcgc cgaggcgctg tcctgagctt gcccgaatgg acgagccttc
      301 tgcgacggcg gcgacgctga cgcattttca cggacgtgct aaagcgcgcg gatagttcga
      361 gaggcccgat gtcagaaaag ctccattcga tcgctcgcgc gatgatttcc gtgtcagaca
      421 agaccggcct cgtggagttc gcgcaagcgc tcgcagcgcg cggtatcgag atcgtctcga
      481 ccggcggcac gcataaggcg cttgtcgcgg caggcatcgc cgcgcgcgat gtctcggaac
      541 tcaccggctt tcccgagatg atggacggcc gcgtgaagac gctgcacccg aaagtgcatg
      601 gcggcctgtt ggccatccgc gaaaacccgg agcatgaggc ggcaatgctg gcgcacgaca
      661 tcgcgccgat cgatcttctc gtcgtcaatc tttatccgtt cgaatcgacg gtcgccaaag
      721 gcgccgacct cgacgaatgt atcgagaata tcgacattgg cggtccggcg atgatccgcg
      781 ccgcatcgaa aaatttcgac gccgtgaacg tcatcgtcga tgtggcggat tatccaaagc
      841 tgcttgtgga acttgaggcg aatgccggca agacgagctt tggcttccgc cgtgctctgg
      901 cgcagaaggc gtttgcgtgc accgccgtct atgatgccgg catttccaat tggtttgccg
      961 cgcagatcgg cgagaaagcg ccgtattatc gcgctttcgg cggcctcaag ctggaagcct
     1021 tgcgttatgg cgaaaaccca catcaggcag cggcgtttta tgccaacgag cagccgagtt
     1081 ttggcctcgc caccgcgaag cagattcagg gcaagcaact ttcctacaac aatgtcggcg
     1141 ataccgatgc agcgctcgaa ctggtcagcg aattcgatcc cgcgaagacc gcggcggtcg
     1201 cgatcatcaa acatgccaat ccgtgcggcg tggcgcaggc gcaaagcctc gacgaggctt
     1261 acaggctcgc gctgcgcagc gatccgatct cggccttcgg cggtattatc gcgctgaacc
     1321 agacgctcga tgccgccacg gcgcgggaga tcatcaaaat ttttaccgaa gtgatcatcg
     1381 caccggacgc gaccgaagag gcgcgcgcga tcatcgcgac gaagctcaat ctacgtctgc
     1441 tgctcaccgg cggcctgccc gatccacgcc gccgcgaact cacctttcgc tcggtcaatg
     1501 gcggcttcct ggtgcagggc cgtgacaacg cgcttgtcga tgacatggaa ttgcaggtcg
     1561 tcaccaaacg cgcgccgacg gcagccgaac ttgccgatct caaattcgcg ttccgcgtcg
     1621 ccaaacacgt caaatcgaac gccatcgtct atgccaaggg cggcgcgacc gttggaatcg
     1681 gtgcaggaca gatgagccgc gtcgattcct cgcgcatcgc cgcgcaaaag gcgctcgaag
     1741 ccgcgcagac ggcggggctt ccggaaagtc tggcgaaagg cgcggttgtc gcgtcggacg
     1801 ccttctttcc cttctccgat ggcttgctgg ctgcggcatc ggcgggcgcg acagccgtga
     1861 ttcagcccgg tggttcgcta cgcgacggcg atgtcatcga agccgccgac gccgagggcc
     1921 ttgcgatggt cttcaccggc gcaaggcatt tccggcatta gaacatcggt tggtgcttcg
     1981 gcgctaagtc ttccggccca tcgccaaaaa cttgtcggcg cgggcgtcac ggagtgcgtc
     2041 gggcgacatg ttgccgaagc tctcaagtgc cgaggcaatg gcattgctcg tcgcggcgat
     2101 ggccgcggct ggatcgcgat gcgcgccgcc gatcggctcg gaaatgatcg catcgatgag
     2161 gccaaagcgc agcaaatcct gcgcggtgat cttcatattg gtcgcggcgt cctgcgccct
     2221 ggccgcatcg cgccagagaa tcgaagccga ggcttcgggc gaggcgacag tgtagatcgc
     2281 atgttcgagc atcagcacgc gattgcaggc agcgatcgca atggccccac ctgagccgcc
     2341 ctcgccgatg acgaccgcga cattcggcac accgagcgac agcgacttat cggtcgagcg
     2401 ggcaatcgcc tcggcctgcc cgcgctcttc cgcatcgacg ccaggaaacg cgcccgccgt
     2461 atcgaccagc gaaatcaccg gcagcgaaaa gcggtcggcg agctccatca gccgaacggc
     2521 cttgcgatag ccttccggcc gcgccatgcc gaaattatgc ttgaggcgtg tttcggtgtc
     2581 gttgcccttc tcttgcccga tgaggcaaac cgaagtgccg cgaaaacgcg cgaagccgcc
     2641 gacgatcgcc gcgtcttcgc caaacagccg gtcgccggcg agcggcgtga aatcggtgac
     2701 gaggccggca acaaaatccg cgcaatgcgg ccgctgcgga tggcgggcga cctgggtctt
     2761 ctgccagggc gtgaggctgg catacagatc ggccaaggcc ttctcggcgc ggatttcgag
     2821 ccgcgtcaga tcctcactcc ccacgccatt ctcgccgtcg ccgacaagcg tgcgcaattc
     2881 ctcgactttg gtgtcgaggt cggcaaccgc tttttcaaaa tcgagatagg tgcgcataga
     2941 gccagtcagg ggaacaaagc ggggagaact ccccgaatta gacgctttca gtcaagcaag
     3001 atggggtttc gcgccagcct cataaaggcg catcgcgaaa gaaga
//
LOCUS       DASWOG010000573         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_308104,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000573 DASWOG010000000
VERSION     DASWOG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308104"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGB93_11670"
     CDS             <1..189
                     /locus_tag="VGB93_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146777.1"
                     /translation="RKTEKAIGTLLDGAHRADECKLKAAFLLDDPGSHARRIANRTFV
                     GTDGERMMKALRCCHTCQ"
     gene            complement(262..909)
                     /locus_tag="VGB93_11675"
     CDS             complement(262..909)
                     /locus_tag="VGB93_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HEY0146778.1"
                     /translation="MRAPRRLKFGVAALLLGSAAPASAHPHVWVTAHEDVIFQPDGKI
                     AAIRNSWVFDDMYSAFAVQGLAKDGQLATKEQLAPLAKTNVESLAEFGYFTFAKNAGE
                     KIDFGPPKDYSLEERPDKLVELSFTVPLKDPLIPGRAFIFSVYDPTFFVDFSLAKTNS
                     VNLLSAPKGCSIGVLGANPLDVQDAKQLSEAFYQNLSPGANFGMKLADRVIVACP"
     gene            1181..>1541
                     /locus_tag="VGB93_11680"
     CDS             1181..>1541
                     /locus_tag="VGB93_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146779.1"
                     /translation="MADVERTQGTAALLSLADKYDLAQNRVFVSGPQAIVRLLLMQAA
                     IDRRAGLNTAGFISGYRGSSLCGLDLNFLRAKKALTARDIICEPGLNEDLAATAIWGA
                     QQDEMRGEGRFDGVIGLW"
BASE COUNT          347 a          451 c          449 g          294 t
ORIGIN      
        1 cgcaagaccg aaaaagccat aggaactttg cttgacggcg cgcatcgcgc cgacgagtgc
       61 aagctaaaag cggctttcct gctcgatgat ccaggcagtc acgccagacg cattgccaac
      121 cgcaccttcg tcggcaccga cggcgaacgg atgatgaaag ccttgcgctg ctgccacact
      181 tgccaatagc agaagcgaga cgaaactaaa tgtgctggta gttagaactc gattcatcag
      241 cgtgcctcga gcctcaatat atcacgggca ggcgacgatg acgcgatcgg ccaatttcat
      301 gccgaagttc gcgcctggcg agagattttg atagaaggct tccgacaatt gtttggcatc
      361 ctggacatcg agcggattgg cgccgaggac tccgatggaa cagcccttgg gcgcgctcaa
      421 gagattgacg gagtttgttt ttgccagact gaaatcgaca aaaaacgtcg gatcgtagac
      481 tgagaaaatg aaagcccgac cgggaatgag cgggtctttc agcggtacgg tgaaactcag
      541 ctccacaagc ttgtccggcc gttcttcgag cgaataatct ttcggcggcc cgaaatcgat
      601 cttctcgcca gcgttcttcg cgaaggtgaa atagccgaac tcagccagtg attcgacatt
      661 ggtcttggcg agcggcgcga gctgctcctt ggtcgccaac tggccgtcct tggcgaggcc
      721 ctggacggcg aaagctgaat acatgtcgtc aaatacccag gaattgcgga tcgccgcgat
      781 cttgccgtct ggttgaaaga tcacatcctc gtgcgccgtt acccaaacat ggggatgcgc
      841 gctcgctgga gccgccgagc cgagcaaaag ggccgcaacg ccaaatttaa gccttcttgg
      901 ggcgcgcatc tttcccgatc aaagggccta aggccgaacg gcagaatcgt taaggcgaca
      961 attgcccggc aaactctggc caaagcgagg cgggggcgat cacttacttg ccccagcggc
     1021 gcggtccatc ccgcaaaaca cggttacaat catttactta aacacaggga agacggtacg
     1081 gcgcagccgc ctaaagggtt ggccgattca gccagctgct ttataccgtt agctcccaat
     1141 ggggccaggc tggatcttcc tgcctggggg caaaggcaaa atggcggacg tcgaaagaac
     1201 ccagggcacg gccgcgttgc tgtcgctcgc cgacaaatac gaccttgccc aaaaccgtgt
     1261 cttcgtctcg gggccgcagg ctattgtccg ccttttactc atgcaagcgg cgatcgaccg
     1321 gcgcgccggg ctgaacaccg ccggcttcat ctccggctat cgcggctcgt cgctctgcgg
     1381 cctcgacctc aatttcctgc gcgccaaaaa agcgctgacg gcgcgcgata tcatctgcga
     1441 gcccggcctc aacgaagacc ttgccgcgac cgcgatctgg ggcgcgcagc aagacgaaat
     1501 gcgcggcgag ggcaggttcg atggcgttat cggcctttgg t
//
LOCUS       DASWOG010000574         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_50003,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000574 DASWOG010000000
VERSION     DASWOG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50003"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGB93_11685"
     CDS             complement(<1..593)
                     /locus_tag="VGB93_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY0146780.1"
                     /translation="MKAICSHFQRLAVLVPLVVCAAGGVSQQALAGQSDIGTSASPLG
                     IPPGPPTAYPDSGVPEGYFFNLRPYGKPVGDYLADKGIYLTGRTLQQGIGLTSGGLRT
                     GTIFEGFNLFGVDLDMGKIAGIKGGSFHFQMNELSGLPFTNRTGSFYVYNRAFALQAG
                     VRLNEFSYEQNFGGLVDIRAGRVPVGTEFDTERVYCE"
     gene            complement(783..2348)
                     /locus_tag="VGB93_11690"
     CDS             complement(783..2348)
                     /locus_tag="VGB93_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0146781.1"
                     /translation="MQTTQGRVQGLTKGGLAEFFGIPYAAPPIGNLRWRPPVARASWA
                     GVLKATAFGPTCAQIENSPFSGPTSTSEDCLYINIFAPAGAVHKKLPVILWSYGGGDF
                     EGESNDYDGSKLALQGHTIVVTFNYRLNIFGFLAHPALDHEGHLFGNYGILDNQFALR
                     WVHDNIAAFGGDAANITIAGQSAGATNVGAEVLSPLDKGFFARAILQSGAMPTLTPLS
                     FAEAKGVAFAVAAGCGAGVTPAVAQCLRKLPAAKILSFAHPPFVANNIADGTILPKVV
                     IDAFESGKFNHVPLMIGNVENEGAYFVAFPEYSEMPRKPLTEAQFQTYLKATYGGNAG
                     PGYSPPAYPIGTIAAVSAHYPLSAYSSPELQWSAVETDGGRLNLSACKLRHVANILAT
                     QTPVYTYEFRDQTAPSYWPPLPGFKFLAYHTGDIQYYWLLYHGGRLGQSHPLNGAQQR
                     LADALVNAWANFAQTGNPNGQGAKPWPRYTPNGLYLDEDIGGLSTFNDAQFSKAHQCD
                     FWDKVIVYRSTAR"
     gene            complement(2682..>3353)
                     /locus_tag="VGB93_11695"
     CDS             complement(2682..>3353)
                     /locus_tag="VGB93_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146782.1"
                     /translation="MPLILGVVKDNPPVANDAPAEVKRSAVRLADVRLLLADKKVWML
                     TGFGAGLLTYLWGLNSWLPTYLQRVRHFDLHHMGIYSALPFILMLFAGLIGGYIADRT
                     GKYALLSSSGLFAAGVLMYIATQVEQPQLAAVIIALSAAAWGFGIPTQYALALRVLPV
                     GTTSTGIGIINGIGNLVGACAPAVIGFIVAGTGSYKAGLLVIVVASVLGSLALLPMLR
                     FRPTT"
BASE COUNT          762 a          988 c          931 g          672 t
ORIGIN      
        1 aattcgcagt acacgcgctc tgtatcgaac tccgtcccaa ccggcacgcg accggcgcgg
       61 atgtcgacaa gtccgccgaa attttgttcg tacgagaatt cgttcaggcg gacgccggcc
      121 tgcaacgcga aggcgcggtt gtagacgtag aagctacccg tgcggttcgt gaacggaagg
      181 cccgataatt cattcatctg gaaatggaac gatccgccct taatacccgc gatcttgccc
      241 atatccagat cgaccccgaa aaggttgaac ccttcgaaga tcgtacccgt cctgaggccg
      301 ccgcttgtaa ggccgatacc ctgctgtaag gtgcggcccg tcagatagat gcctttgtcg
      361 gccaaataat cgccgaccgg ctttccatac ggacgcagat tgaagaaata gccctctggc
      421 acgccggagt ccggataagc cgtgggcgga ccgggtggaa tgccgagcgg cgacgcgctt
      481 gtgccgatgt cagattggcc tgcgagtgcc tgttgcgaca caccccccgc ggcacagacg
      541 accaacggca ccaggaccgc aagccgctga aaatgtgagc agatcgcttt catataaccc
      601 cctcagatca cgtaaaatgc atagattctg gttttttctt caaccgggta actatctcgc
      661 acgactcatg ccaaaattgt catgttgtct gacaatcaac atcaccctta tatgcgacag
      721 cttagcgcat gacaaatctt cgttagcttc caagagggcc gtcccggcgc tctgcctgcg
      781 atttaccgag ccgtcgaccg gtaaacgatc accttgtccc aaaagtcgca ttggtgcgcc
      841 ttcgagaatt gcgcatcgtt gaacgttgaa agaccgccaa tatcttcgtc gagataaagt
      901 ccgttcggcg tataacgcgg ccacggcttc gccccctgcc catttgggtt gccggtttgc
      961 gcaaaatttg cccaagcatt gacgagtgca tcagcaagtc tttgctgcgc gccattcaag
     1021 ggatgcgact ggccgagacg gccgccgtga taaagcagcc agtaatattg aatgtcgccc
     1081 gtgtgatagg cgaggaattt gaatcccggc agcggcggcc aataagatgg cgcggtctgg
     1141 tcgcgaaatt cgtacgtata gaccggcgtt tgcgtcgcca gaatatttgc gacgtgccgt
     1201 agtttgcagg ccgacaaatt caaacggccg ccgtctgttt cgacggcgct ccattgcaat
     1261 tccggacttg aataggcgct gagcggatag tgtgcactca ccgccgcgat cgtgccaatc
     1321 ggataggcag gcggtgagta cccagggccg gcattgccgc cataggtggc ttttagatac
     1381 gtttgaaact gcgcttcggt aagcggcttg cgcggcattt cgctgtattc aggaaacgcg
     1441 acaaaataag cgccttcgtt ttcaacattg ccgatcatca gcggcacatg gttgaacttg
     1501 ccactttcaa aggcatcgat aaccaccttc ggcaagatcg tgccatcggc gatattgttg
     1561 gccacgaaag gcggatgcgc gaaggagagg attttcgcgg ccggaagctt gcgcagacat
     1621 tgtgcaacgg ctggcgttac gccagcgccg catccggcgg ccaccgcgaa cgcgacgccc
     1681 ttcgcttcgg cgaaggacaa cggcgtcaag gtcggcatcg cgccactttg caggatcgcg
     1741 cgcgcgaaaa aacctttatc gagcggcgaa agaacctcgg cgccaacatt tgttgcgcca
     1801 gccgattgac cggcaatcgt tatatttgcc gcgtcgccgc caaaggccgc gatattgtca
     1861 tgcacccagc gcagcgcaaa ttgattatcg aggatgccgt aattgccgaa taaatgaccc
     1921 tcatggtcaa gcgccggatg agccagaaag ccgaaaatgt tgaggcgata attgaaggtg
     1981 acgacgatag tatgcccttg aagcgcgagc ttgcttccat cgtagtcatt gctttcccct
     2041 tcaaagtcgc cgccgccata gctccagaga ataaccggca attttttatg caccgccccc
     2101 gccggcgcaa aaatattgat gtaaagacaa tcctcgctcg tcgatgtcgg cccggaaaac
     2161 ggcgaattct cgatctgcgc gcaagttggc ccgaaggcgg tcgctttgag aacgcctgcc
     2221 cacgatgcac gcgcgaccgg tggccgccaa cgcaggtttc cgatcggcgg cgccgcatac
     2281 ggaatgccga aaaattccgc gaggccgccc ttcgtgagtc cctgaacacg cccctgcgtg
     2341 gtttgcacta tggggccaag atcatgtcgt tctgagtcgc tggcggcggc aggtgttctt
     2401 gtctctgcgg cggcacaaac aaccaacatc gcagccacag ccaaccgctg gagcgataga
     2461 ccgatcgcca tcatgtcatc ctctcgtgtt ttcaaagccg aatggtggcg tcttccgaca
     2521 gccagtccca ctcattcacg cacgcatatt gtctcgttgt cagacacacc ttgcgcggat
     2581 cgtgcgcgaa tttatcaaaa ggcccaaggt gcgttggatg cgccctgggc catttcgagt
     2641 tcaagttttc gaatttcggc aatcgcagcc gcgcgtcgcg ttcacgtcgt cggcctgaag
     2701 cgcaacatcg gcaaaagcgc cagcgatccg agcacacttg cgacgacgat gacgagcagg
     2761 ccagctttat agctgcccgt ccccgcgacg atgaagccga tcacagccgg ggcgcaggcg
     2821 ccgacaagat tgccaatacc attgatgatt ccgatacctg tcgacgtcgt gcccactgga
     2881 agcacgcgta gcgccagtgc atattgcgtg ggaatgccga agccccaggc cgcagcgctc
     2941 aatgcaataa tcaccgccgc gagttgcggc tgctccacct gcgtcgcgat atacatcagg
     3001 acaccagctg caaacaggcc ggaactgctc aagagcgcat atttgcctgt ccggtcggcg
     3061 atatagccgc cgatcagacc tgcgaacaac atgaggatga aaggaagcgc cgagtaaatc
     3121 cccatatggt gcagatcgaa atgacggacg cgctgcagat aggtcggcag ccagctattc
     3181 aggccccaga gataggtcag cagacccgcg ccaaatccgg tcagcatcca gacctttttg
     3241 tcggcaagca aaaggcgcac atcggcgaga cggacggcgc tgcgcttaac ttcggcaggt
     3301 gcatcgttcg ccaccggcgg gttatccttc acgacgccaa gaatcagcgg cat
//
LOCUS       DASWOG010000575         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_50006,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000575 DASWOG010000000
VERSION     DASWOG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50006"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VGB93_11700"
     CDS             complement(<1..1319)
                     /locus_tag="VGB93_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146783.1"
                     /translation="MAQSDQLTNARVKPQLALRIGVTGARNLATTHLSHIHAKVRAAL
                     TCFSDEMNSIARERAVARFYVSDDKKTCAPLLRIISPLALGADRLVAEEALALGYKLY
                     VPMPFSRTEYEKDFAGTDPAKTPHALPITAAEDLDQFRTLLARAGEDWMSLDGDYDKE
                     KPDRDEQRNRAYEAVGRFVVRNSDIVIAIWNGELAAGRGGTQEVVEYAANVGVPVWWI
                     HATKDIEPHWIADIHDARNPSGSPKAAHKIHAYLRRQILEPKPVPRGHQTGVVSIARW
                     WQSKTVEPAQDYFNEQPNRPWRWSGIYNEMMLFASGCQAAWTRPTEPKSDAGIYWFGL
                     YQPADARGSEYTARYRSSYVWIFTLTALSLLSGAIANFFHKRDITIFGHIIESRVSVT
                     TFAGIEFFLLSVTFCIAVLALRRDWHEHSIEYRLLAELCRKQQVLAPL"
     gene            1508..>1946
                     /locus_tag="VGB93_11705"
     CDS             1508..>1946
                     /locus_tag="VGB93_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146784.1"
                     /translation="MPKYSWMALVGVSLLSGCGSMTGPPSITASRTFYNEVIHDTSSE
                     QLLLNIVRAKNYETPNFIDVSEADATVSFTSGVNGGLSGIGAVPGIHSTSAGTIAGQV
                     GGVTGNFNYADSALVRYAPVQGYPLIQQASSPIAPVSIVHMFNS"
BASE COUNT          414 a          569 c          543 g          420 t
ORIGIN      
        1 agcggcgcga gcacctgctg tttgcggcaa agttcagcga gcaggcggta ttcgatcgag
       61 tgctcatgcc aatctcgacg caacgccaaa actgcgatgc agaaagtcac agacagaaga
      121 aagaactcga tgccggcgaa agtcgtgacg ctcacccggc tttcaattat atggccaaag
      181 atcgtgatat ctcgcttgtg aaaaaaattg gcgatcgcac cggacagcaa cgacaacgcg
      241 gtaagcgtga atatccagac ataactcgaa cggtagcgcg cggtatattc gctgccacgc
      301 gcatccgccg gctgataaag cccaaaccaa taaatacccg cgtcgctttt cggctctgtt
      361 ggccgcgtcc atgctgcctg gcagcccgaa gcgaagagca tcatttcgtt atagattcca
      421 gaccaacgcc acggtcgatt tggctgctcg ttaaaataat cctgcgccgg ctcgactgtc
      481 ttggattgcc accagcgcgc aatgctcacg acgccagttt gatgtccgcg cggaacgggc
      541 ttcggttcta aaatctgccg gcgcaggtaa gcgtgaatct tatgcgctgc tttcggcgaa
      601 cctgacggat tgcgcgcatc gtggatatcg gcgatccaat gcggctcgat atcttttgtc
      661 gcgtgaatcc accagacggg cacgccgaca tttgcggcat attcgacgac ttcctgtgtg
      721 ccgcctcgac cggccgcaag ttcgccgttc cagatcgcga tgacaatatc gctgttgcga
      781 acgacgaaac gaccgacggc ctcataagcg cggttgcgct gctcatcgcg atccggcttt
      841 tccttgtcgt aatcgccgtc gagcgacatc cagtcttccc ccgcgcgcgc caggagcgtc
      901 cggaactggt caagatcctc cgctgccgtt atcggcaggg cgtggggcgt ttttgctgga
      961 tccgttccag caaagtcctt ctcatattcg gtacgcgaaa atggcattgg cacataaagc
     1021 ttataaccga gcgcaagtgc ctcttccgcg acaagacgat cggcgccgag ggcaagcggc
     1081 gaaatgatac gcagaagcgg tgcgcatgtt tttttatcgt cgcttacata gaaccgcgcc
     1141 acggcgcgct cccgcgcgat cgaattcatt tcgtcgctga aacatgtcaa cgccgcgcgc
     1201 actttggcgt gaatatggga aaggtgcgtc gtcgcgagat tgcgcgcgcc cgtgacacca
     1261 atgcgcagcg cgagctgcgg cttcacccgc gcatttgtca gctgatccga ttgagccaag
     1321 ggcacaccgc aagcaacgat ctagcttcgg cgatttgtgc catggcgcat cggcttaaac
     1381 aagtcgacgc ggccaaatca gatgcaaaat tacaacattc ggtaacaatg ctttcgctgc
     1441 cattgactca aattcgcgct gccgatcatg tcaatggctc cggttgcggt gtgtatcggg
     1501 ggttgccatg ccgaagtaca gctggatggc gctcgtcggc gtaagccttt tatccggctg
     1561 cggttcgatg acggggccgc catcgattac ggccagtcgg acgttttaca acgaagtaat
     1621 tcacgacacg tcgtccgagc aattgttgct caatatcgtc cgcgccaaga actacgagac
     1681 gccgaatttc atcgatgtct cggaggccga tgccacggtc tcgtttacgt ctggtgtcaa
     1741 cggcggcctt tcgggtatcg gggcggtgcc agggatacac tctaccagcg ccggaacgat
     1801 tgccggccag gtgggcggcg tgaccggcaa tttcaattat gccgattccg cgcttgtccg
     1861 ctacgcgccc gtccaaggct atccgctcat ccagcaagcg tcgtcgccga tcgcgccggt
     1921 ttcgatcgtg catatgttca attccg
//
LOCUS       DASWOG010000576         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_14245,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000576 DASWOG010000000
VERSION     DASWOG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14245"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGB93_11710"
     CDS             complement(<1..235)
                     /locus_tag="VGB93_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEY0146785.1"
                     /translation="MVEPSAPETSPIAIDLHKELAETQVGEILDELDRELIGLVPVKT
                     RIRQIAALLVVDRVRKRMGFVKETPTLHMSFPGN"
     gene            complement(341..745)
                     /locus_tag="VGB93_11715"
     CDS             complement(341..745)
                     /locus_tag="VGB93_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEY0146786.1"
                     /translation="MRVTQGCFSYLPELSDADIRTQVQYCIDKGWAVNIEFTDDPHPR
                     NTYWEMWGLPMFDIKDAAAVMLELDACRKVHAGRRYIRLSGFDSSHGWESVRISFIVG
                     RPAEEPGFILTRNELGGQAMQYTLQARPLTGA"
     gene            complement(813..2279)
                     /locus_tag="VGB93_11720"
     CDS             complement(813..2279)
                     /locus_tag="VGB93_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEY0146787.1"
                     /translation="MNVLDKKTPGEKRNRYSAGVMEYRDMGYWQPDYEPKDTDILAAF
                     RVTPQDGVDPIEAAAAVAGESSTATWTVVWTDRLTASEKYRAKCYRVDPVPNAPGSYF
                     AFIAYDLDLFEPGSIANLSASIIGNVFGFKPLKGLRLEDMRFPVAYVKTFQGPATGIV
                     VERERLDKFGRPLLGATVKPKLGLSGRNYGRVVYEALKGGLDFTKDDENINSQPFMHW
                     RERFLYCMEAVAKAEALTGEIKGTYLNVTAATMEDMYERADFAKQLGSVIVMIDLVVG
                     YTAIQSMAKWARKNNIVLHLHRAGHSTYTRQKSHGVSFRVIAKWMRLAGVDHIHAGTV
                     VGKLEGDPNNVRGYYDICREDYNPVALEHGIFFEQHWASLNKLMPVASGGIHAGQMHQ
                     LIDLLGEDCVLQFGGGTIGHPMGIEAGATANRVALEAIIFARNARRDLLNEGQEILEQ
                     AAKNCLPLRQALETWKDVTFNYSSTDTPDFVPTATAAQ"
     gene            complement(2342..>2964)
                     /locus_tag="VGB93_11725"
     CDS             complement(2342..>2964)
                     /locus_tag="VGB93_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0146788.1"
                     /translation="DSIGLGEDGPTHQPVETLASLRVIPHLNVFRPADAIETAEAWEL
                     ALQLSEAPSVLALSRQNLPVLRRTHTNENLSAKGAYVLREVAGERDVTLIATGSEVSL
                     AVAAADELAKDSVKAAVISLPCFELFAAQEPGYRAAVLGQAPRIGIEAALRQGWGAVL
                     APEDYFIGMKDFGASAPAADLYKHFGITVEAIVSAARAAAGHGTAS"
BASE COUNT          583 a          878 c          889 g          614 t
ORIGIN      
        1 gattgccggg gaagctcatg tgtagcgtcg gcgtttcctt cacaaagccc atgcgtttgc
       61 gcacgcggtc gacgacgagc agagcggcga tctggcggat gcgcgtcttc accggcacga
      121 ggccgatgag ttcgcggtcg agctcgtcga gaatttcgcc gacctgcgtc tcggccagct
      181 ccttgtgcag gtcgatcgcg atgggtgatg tttcgggtgc ggaaggctcg accattttat
      241 tctgagctct gtgtggtgcc cttcattgcg agcgaagcga agcagtcaag caatcgaatc
      301 tggattgctt cgtcgcgatg ctccccgcaa tgatgatcca tcacgcgcct gtcaacgggc
      361 gcgcctgcaa cgtatattgc attgcctggc cgccaagctc gttgcgggtc aggatgaagc
      421 ccggctcttc cgcaggccgg ccgacgatga aggatatgcg cacgctctcc caaccatgcg
      481 aagagtcgaa gccggaaagg cgaatatatc tgcggcccgc atgcaccttg cggcaggcgt
      541 cgagttcgag catcaccgcc gccgcatcct tgatgtcaaa catcggcaga ccccacattt
      601 cccaataggt attgcgcggg tgcgggtcgt cggtgaattc gatgttcacc gcccatcctt
      661 tgtcgatgca atattggact tgggtgcgta tgtccgcatc gctcaattcc ggcaggtacg
      721 aaaagcagcc ttgggtcacg cgcatcttcg ttctcctatt catgtcagcg tatgatcttg
      781 tccgaaaagt ctgcaacttt tcgggatcac gcttactgcg ccgcggttgc ggtcggcaca
      841 aaatccggcg tgtcggtcga tgagtaattg aaggtcacgt ccttccacgt ctcgagcgcc
      901 tggcgcagcg gcagacagtt ctttgccgcc tgttcaagga tttcctgacc ttcgttcagc
      961 aaatcgcgcc tggcgttgcg ggcaaaaatt atcgcttcga gcgccactcg attggcggtc
     1021 gcaccagctt cgattcccat cggatggcca atcgtgccgc cgccgaattg tagcacgcaa
     1081 tcttcaccaa gcagatcgat gagctgatgc atctgtccgg cgtggatgcc gcccgaggca
     1141 acgggcatca gcttgttgag cgatgcccaa tgttgctcga agaaaatgcc gtgttcgagc
     1201 gcaaccgggt tataatcctc gcggcagatg tcgtaatagc cgcgcacgtt gttggggtcg
     1261 ccctcaagct tgccgacaac cgtgccggca tggatgtgat cgacgccggc caggcgcatc
     1321 catttggcga tgacgcgaaa tgagacgcca tgcgatttct gccgcgtata ggtcgaatgg
     1381 ccggcgcgat gcagatgcag gacgatatta ttcttgcgtg cccatttcgc catggactgg
     1441 atcgccgtat agccgacaac cagatcgatc atcacgatga cggagccgag ctgcttggcg
     1501 aaatcagcac gctcgtacat gtcctccatc gtggccgccg tgacattgag ataagtacct
     1561 tttatctctc ccgttaacgc ctcggccttc gccacggcct ccatgcaata gaggaagcgc
     1621 tcgcgccaat gcatgaaggg ctgcgaattg atgttctcat cgtccttggt aaagtcgagg
     1681 ccgcccttca gcgcctcgta gacgacacga ccgtaattgc ggccggagag gccgagcttc
     1741 ggtttcacag ttgcgccgag cagcggccgg ccgaatttat caaggcgctc gcgttcgacg
     1801 acgatgccgg tcgcggggcc ctgaaaggtc ttcacatagg cgacgggaaa gcgcatgtct
     1861 tcgagccgca ggcccttgag cggtttgaag ccgaaaacat tgccgatgat cgaggcggac
     1921 agattggcaa tcgagcccgg ctcgaagaga tcgagatcat aggcgatgaa ggcgaaatag
     1981 gagccgggcg catttggcac cggatcgaca cgatagcatt tagcgcggta tttttcgctg
     2041 gcggtcagcc gatccgtcca gacaacggtc caggtcgcgg ttgaggattc gccggcaacg
     2101 gcggctgccg cctcgatcgg atcgacgcca tcctgcggcg tgacgcggaa ggccgcgaga
     2161 atgtcggtgt ccttcggctc gtaatcgggc tgccaatagc ccatgtcgcg atattccatc
     2221 acacccgcgg aatagcggtt gcgcttctcg ccgggcgttt tcttgtcgag cacgttcatg
     2281 atcgcgactc ctcaaaggtt tatgcgcccc cgcggcaagt agatccctgg ctcgtaaatc
     2341 cctagctcgc agttccgtgc ccggctgcgg cacgcgccgc gctcacgatc gcttcaaccg
     2401 tgatgccgaa gtgtttgtag agatcggcgg ctggcgcgct ggcgccgaaa tctttcatgc
     2461 cgatgaaata atcctcaggt gcgagcacgg cgccccatcc ctggcgcaat gcagcctcta
     2521 tgccgatccg aggtgcctga ccgagaacgg cggcgcgata gccgggttcc tgcgcggcga
     2581 aaagttcaaa acacggcagc gagatgaccg cggccttgac actatctttt gcgagttcgt
     2641 ccgctgcagc gaccgcgagc gagacctcgg agccagtggc gatgagcgtg acatcgcgtt
     2701 cgccagccac ttcgcgcagc acataggcgc ccttcgcgga aaggttttcg ttggtatgcg
     2761 tgcggcgcag aaccggcaaa ttttgccgcg acagggccag cacagacggc gcctcggaaa
     2821 gctgcaaagc aagctcccaa gcctccgccg tctcgatcgc atcggctgga cggaagacat
     2881 ttagatgcgg aatcacccgc aacgaggcga gcgtctcgac tggctgatgc gtcggcccat
     2941 cttcgccgag accgatggaa tcat
//
LOCUS       DASWOG010000577         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_50039,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000577 DASWOG010000000
VERSION     DASWOG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50039"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..533)
                     /locus_tag="VGB93_11730"
     CDS             complement(156..533)
                     /locus_tag="VGB93_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0146789.1"
                     /translation="MKQILVVDDSTVIRKVARRILEGMNFQTVEAEDGRKALNACSFF
                     MPDAILLDWNMPVMDGFEFLHELRKMPEGLKPKVVFCTTENDVAHIARAIHAGADEYI
                     MKPFDKQILQSKLEEIGLLDAAA"
     gene            complement(575..1066)
                     /locus_tag="VGB93_11735"
     CDS             complement(575..1066)
                     /locus_tag="VGB93_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0146790.1"
                     /translation="MDYRQDPARSSSAPGGAEDDAQIRCFVVFVGGEPFGLCVDYVQT
                     IFGIDAVTPVPLGPREIIGLVNLRGKIVTAVSLRRRLDLPDPADTRSMLAVGMDYRGE
                     NFALVVDEVGDVLHLSPQSRISLPPHLNDRRAKFTKAAYRLETGILSLLDVAAIFDFP
                     RHA"
     gene            complement(1069..3597)
                     /locus_tag="VGB93_11740"
     CDS             complement(1069..3597)
                     /locus_tag="VGB93_11740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0146791.1"
                     /translation="MDDLLSDFLVETSEQIDAAGEQLVLFERDPSDREAIIRIFRLFH
                     TIKGTCGFLSLSRLELLTHVTESLISKLRDGAPATSETVSLILTAVDRVKAILANLTE
                     VGKEPDGDDHDLIAALESDASATSRPIESAPLAAQPRADAVANTPPRARPIPSNRADS
                     IRVSVGALERIMILVSELVLTRNQLLEITRHQEDAIIKTPLQRLSGLTSDLQDAVMRA
                     RMQSVGRVFAGLPRLIRELSVDLKKKFRLVTEGSDTELDRQLIELIRDPLTHLLRNCA
                     DHGIESPEDRVAQGKPEEGTIRVAAAHEAGYITIDISDDGRGLDMQKIREKAVAKGLV
                     SAAELRGLSDEDVSRFIFAPDFSTAATVTSISGRGVGMDIVRDNIEAIGGTISLTTMA
                     GKGTRFTLRIPLTLAITPALIVEVSGHRFALPQHSVVEAVGLEEAGAHALELLQGSLV
                     LRLRDETLPVASLKSLLQLDYSAPAPAGELVIVMRVHNRVFGILVDAVLDVQEIVVKP
                     LGSSLSDLRAFSGHTILGNGSVVLILDPSGIASRMGFESVSDYAVGRAVETYVPADEM
                     TRFVLFRAGPGAPKALPLSLVVRIESIQAKDVHTSDGRMVVQHQSQLMPLVPLSPDAS
                     SQLQDVNPVLVLGVGGESMGLLIEEIIDVIDAPLDIEITSVSPGIIGTAEIRGEIAEV
                     LDATYFLQLGRPAAYSRGVAHQFRVLLVDDKPFFRDMLAPLIIAGGYRVNTAASAAEA
                     LAMFEKGAQFDAVVTDTDMPNMNGYEMARQLTQEPRHQGLPIVALAAHASPNVLQAAK
                     ESGMYGAVGKFDRTALIRILGEILEVRHLNRHELERSVIGSVAA"
BASE COUNT          777 a         1073 c         1134 g          740 t
ORIGIN      
        1 gggccgcccg tcgaagcgcc aatcacgatc tcttcgggcg gtacggaaag ttcaaatccc
       61 atgagcggat gcaaatttgc cggcgcagat atttcgccca tggcaccaaa atccatatcg
      121 ctgccgtgtt gtctcatgcc ggcgggacca ctgtctcacg cggccgcgtc gagcaagccg
      181 atttcttcaa gcttggactg caggatctgc ttgtcgaagg gcttcatgat gtattcgtcg
      241 gccccggcat ggatcgcacg cgcaatatgc gcgacgtcat tttccgtcgt gcagaagacg
      301 actttgggct tcaagccttc gggcatcttg cgcagttcgt gcaaaaactc gaacccgtcc
      361 atcaccggca tgttccagtc gagcaagatc gcatccggca tgaagaaact gcaggcgttg
      421 agcgccttgc ggccgtcctc ggcttcgaca gtctggaaat tcatgccttc aaggatgcgc
      481 cgcgccacct ttcggatgac ggtcgaatcg tcgaccacca aaatttgctt cattgtcgtg
      541 tccccgatct cttccatttg actcatccgt tgcgttacgc gtggcgcgga aaatcgaaaa
      601 tcgcagcaac atcgagcaac gacagaattc cggtttccag acgatacgcg gccttggtaa
      661 actttgccct gcgatcgttg agatgtggcg gcagggagat tcgtgattgc gggctaagat
      721 gcagaacatc gccgacctca tcgacgacga gggcgaaatt ttcgccacgg tagtccatgc
      781 cgaccgcgag cattgagcgg gtgtcggctg gatcgggcaa atccagtcgc cggcgcaggc
      841 tgaccgcggt aacgattttg ccgcgcaaat tgacgaggcc gatgatttcc ctcggcccga
      901 gcggcaccgg cgtgacggca tcgattccga aaatggtctg cacgtaatcg acacaaagcc
      961 cgaatggctc gccgccgacg aagacgacga agcaccggat ttgcgcatcg tcctcggcgc
     1021 cgccgggggc gcttgatgat cgcgcaggat cttgtctgta gtccattgtc atgcagcaac
     1081 cgatccgatg acgcttctct caagctcgtg tctgttgagg tggcgcacct caagaatttc
     1141 gccgagtatc ctgatcagcg cggtgcgatc gaattttccg acagcaccat acatgccact
     1201 ttcctttgcc gcctgcagga cgttcggcga agcatgcgct gcaagcgcga cgatcggcag
     1261 accttgatga cgaggttctt gggtcagttg gcgcgccatc tcgtaaccgt tcatgttggg
     1321 catgtcggtg tcggttacga cggcgtcgaa ttgcgcgccc ttctcgaaca tcgcgagcgc
     1381 ttccgcggcg gaggcggcgg tgttgacccg ataccccccg gcgatgatca ggggcgccag
     1441 catgtcgcga aagaacggtt tgtcatcgac gaggagcaca cggaactggt gcgccacgcc
     1501 gcgtgaatat gcggccggac ggccgagctg cagaaaatag gtcgcgtcga ggacttccgc
     1561 gatctcgccg cggatttcgg cggtgccgat gatgcccggc gacacgctgg tgatttcgat
     1621 atccagaggc gcgtcgatca catctatgat ttcctcgatc aaaagcccca tgctctcgcc
     1681 accgacaccg agtaccagaa ccgggttgac atcctgcaat tgagagctcg cgtcggggga
     1741 gaggggcacg agcggcatca attgcgactg atgctggacg accatacggc catcggaggt
     1801 gtgaacgtct ttggcctgga tcgactcgat ccgcacaacc agggaaagcg gcagcgcttt
     1861 cggcgcgccc gggcccgcgc ggaacaacac aaaacgcgtc atttcatcgg cgggaacgta
     1921 agtttcgaca gcgcggccga ccgcgtaatc gctgacactc tcgaagccca tccgtgaagc
     1981 aatgccgctg gggtcaagga tcagcaccac ggagccgttg ccaagtatcg tgtgcccgga
     2041 aaaagcacga aggtcggaca acgaactgcc gagcggcttc acaacaattt cctgcacgtc
     2101 caatacggca tcgacgagaa tgccgaatac gcggttatgg acccgcatga cgatcacgag
     2161 ttcgccggcg ggcgctggcg cgctgtagtc gagttgcaga agcgacttga gcgacgcgac
     2221 aggtaaagtc tcgtcgcgca gccgaaggac caggctgcct tgtaaaagct caagcgcgtg
     2281 cgcgccggcc tcttccagac caactgcctc gacgacggaa tgctgcggca gcgcgaagcg
     2341 gtggccgctg acctcaacga tcaacgccgg cgtgattgcc agggtgagtg gaattctgag
     2401 cgtgaagcgc gtgcctttgc cggccatcgt cgtcaacgag atcgtgccgc cgatcgcctc
     2461 gatattgtcg cgcacgatat ccatgcccac gccgcggcca gaaatacttg taacggtcgc
     2521 cgcagttgag aagtccggcg cgaagataaa gcgacttacg tcctcatctg aaagcccacg
     2581 aagctccgcc gcggatacga ggcccttggc gacggccttc tcgcgaattt tctgcatgtc
     2641 gagaccgcgg ccgtcgtcgg agatatcgat ggtgatgtac ccagcctcgt gcgcggcggc
     2701 gactcggata gtgccttcct cgggcttgcc ctgcgcgaca cggtcttccg gcgactcgat
     2761 gccgtgatcg gcacaattgc gcagaagatg tgtcaggggg tcgcgtatga gttcgatgag
     2821 ctggcggtcg agttccgtgt cggagccttc agtgaccagc cgaaatttct ttttaagatc
     2881 gacggaaagc tcgcggatga gccggggcag gcctgcgaaa acgcgtccga cggattgcat
     2941 gcgcgcacgc atgaccgcgt cttgcagatc gcttgtaaga ccggagagac gctgcagcgg
     3001 cgtcttgatg attgcatctt cctgatgccg ggtgatttct aaaagctggt tgcgcgtcag
     3061 cacaagttcg gagacgagga tcatgatcct ctccaaagcg ccgaccgaca cgcgaatgct
     3121 atctgcccgg ttcgatggta tgggccgcgc ccgggggggg gtgttggcga cagcgtccgc
     3181 cctaggctgc gctgcgagcg gcgcgctttc gattgggcga gatgtagcgc tcgcgtcgga
     3241 ttccagcgcc gcgattaagt catggtcgtc accgtcgggc tctttgccga cttccgtaag
     3301 gttagcgaga atggctttca cacgatcgac cgcagtcaga atcagcgaga cggtctccga
     3361 tgtcgccggc gcgccgtcgc gcaatttgct gatcagactt tccgtaacgt gtgtgagcag
     3421 ttccagccga gagaggctga gaaaaccgca agttcccttg atcgtgtgga ataggcggaa
     3481 gatacggata atggcttcgc gatcggaggg gtcgcgctca aacagaacaa gttgttcgcc
     3541 ggcggcgtcg atttgctcgc tggtttcgac aaggaaatcg ctaagcaggt cgtccatcgc
     3601 tgcgccaaat ctccagccaa ggacaagtcc gggcgaagaa ttattgcgct gaatgggtta
     3661 aaaagcgttg aaaaaacgcg gccattcaaa gccggttacg aataaaagac gcaataattt
     3721 agcg
//
LOCUS       DASWOG010000578         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_50095,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000578 DASWOG010000000
VERSION     DASWOG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50095"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VGB93_11745"
     CDS             <1..687
                     /locus_tag="VGB93_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0146792.1"
                     /translation="IWFPLAQPNLKLNGIWDAICSAAGVARAPSSTAPVLSAFTTSGV
                     IVTTHVLEHLGASAIGEGATLAQRCAICHGPEGMSGANAPNLAGQHAIVIYKELNDFQ
                     SGARTNGVMTPFAGLISKPEVLELAAYYSQLPPPAVAGTPLPPPKIVVEGEPMRDIAP
                     CEACHGGMAEKVGAPRLDGQPAAYIKEQLLAFASGARHNDISEQMRNIARQMTPDEMD
                     IAAHYYASLR"
     gene            complement(741..1337)
                     /locus_tag="VGB93_11750"
     CDS             complement(741..1337)
                     /locus_tag="VGB93_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0146793.1"
                     /translation="MSNLPYAPQQASSMDVTEAIYKRRATRNFTTAPVAVDILTKLVD
                     AAIQAPSAFNAQPWHFTIIRNPALLDRISAAAKPYMLSTLDPKSAPNGIREHLSNPDF
                     HVFYHAPAMILISGKDSEWAAEDVGLAAENLMLAAHSAGLGTCWIGFAQPWLKTPEGK
                     RAVELNSIYIPIAPIIVGYARETPPPVPRNPPLLQWID"
     gene            complement(1334..2041)
                     /locus_tag="VGB93_11755"
     CDS             complement(1334..2041)
                     /locus_tag="VGB93_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0146794.1"
                     /translation="MSTQLAYSVDHATPFPPLVVARNPWMKTPGERPERGSALGMAGS
                     VLHFGQDREIYSEGAEADLFFKVVSGVVRTCKFLSDGRRQIDAFYKAGDVFGFEPGGL
                     HGFSAEAVRDCTLISYRRRGLEALAANDEGLLAQLFSYAMQSLARAQEHALLLGRRSA
                     TEKVAAFLIDGIDESNGGRIVKLAMTRQDIADYLGLTIETVSRTLSQLERDALIEIPA
                     ARQIHLKNPSALRELNA"
     gene            complement(2158..2535)
                     /locus_tag="VGB93_11760"
     CDS             complement(2158..2535)
                     /locus_tag="VGB93_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0146795.1"
                     /translation="MNSGSDIILVVDDDFAVRESLKFALELEGLVVHACGSGMELLRH
                     PDLSRARCLILDYRMPVIDGFEVMERLDALQIALPVILITSAATDALRKRASKAGVRH
                     VVEKPLSDSALLENIHDVLGLSS"
     gene            complement(2651..3268)
                     /gene="fixJ"
                     /locus_tag="VGB93_11765"
     CDS             complement(2651..3268)
                     /gene="fixJ"
                     /locus_tag="VGB93_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEY0146796.1"
                     /translation="MSNSVVHLIDDDDGVRQSLAFLLATAGLAVRVYESGVAFLAALG
                     TLQPGCIVSDVRMPGIDGLELQRRLKALGVHLPVIIMTGHADVPLAVEAMKAGAVDFI
                     EKPFDDELLLSAIRLALDRYNELGHHETEVVQIQTRLQSLSPRERQVLDGLLAGYPNK
                     TIAYDLKLSPRTVEVHRANVMMKMGADSLSGLVRMAVQAKVLPGG"
     gene            complement(3261..>3860)
                     /locus_tag="VGB93_11770"
     CDS             complement(3261..>3860)
                     /locus_tag="VGB93_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146797.1"
                     /translation="ESGNSDNIPMAREAMDRAADQALRAGQIIRRLREFVARGESERQ
                     IENLVRLIEEASALALVGIKETGVRVSFLFESRSTLVLADKIQIQQVLLNLMRNAIEA
                     MQEVERRELTVSTYKEDNETVRIDVRDTGPGIAPEITHQLFQPFITTKRQGMGVGLSI
                     SRTIVESHGGRLWAEPNADGGTIFHLTLKTIAGEGLGDE"
BASE COUNT          855 a         1156 c         1072 g          777 t
ORIGIN      
        1 atttggtttc ccttggcgca accaaatctg aaactgaacg gcatctggga tgcgatctgt
       61 agtgctgcgg gcgttgcgcg cgcgccgtcg agtacggcgc ccgtattgtc cgcgtttacg
      121 acatctggcg tcatcgtcac gacacatgtg ctggaacatt tgggcgcgtc cgccatcggc
      181 gaaggtgcga cgcttgcgca gcgttgcgcg atctgccatg ggccggaagg catgagcggc
      241 gccaacgccc ccaaccttgc aggccagcat gcgatcgtga tctacaagga actgaacgat
      301 ttccaatccg gtgcgcggac caatggcgtc atgacgcctt tcgcgggcct tatcagcaag
      361 ccggaagttc tagaattagc ggcttactat tcgcagctgc caccacctgc tgtagccggt
      421 acgccacttc ccccgccgaa gatcgtcgtc gaaggcgagc caatgcgcga tatcgcgccc
      481 tgcgaagctt gccacggcgg catggccgaa aaagtcggtg cgccgcggct tgacggacag
      541 ccggccgctt acatcaagga gcaattgctc gcctttgcat caggcgcccg ccataacgac
      601 atcagcgagc aaatgcgcaa tatcgcccgt cagatgacgc ccgatgaaat ggatattgct
      661 gcgcattatt acgcatcact gcgctgagcg actaccacat aggctgtcgc tcagcccctg
      721 ctctgtgagc gggagatcgg ctagtcgatc cactgaagaa gcggaggatt gcgcgggaca
      781 ggtggcggtg tttcgcgcgc atagccaacg ataattggtg cgatcgggat atagatcgag
      841 ttgagctcga ccgcccgctt accttcaggc gttttgagcc aaggctgtgc aaatccgatc
      901 cagcacgtcc ccaaccctgc ggaatgagcc gcgagcataa gattttctgc cgcgaggccg
      961 acgtcctcgg ctgcccattc gctgtctttt cccgagatca ggatcatcgc cggcgcatga
     1021 taaaagacgt ggaagtcggg attgctgaga tgttcgcgga ttccattcgg cgctgattta
     1081 ggatccagcg tactcagcat gtaaggtttc gcggcggccg atatgcgatc aagaagcgcc
     1141 ggatttcgta taatcgtaaa atgccatggc tgggcattga acgcactcgg tgcctggatc
     1201 gcggcatcga caagcttcgt tagaatatca acggcgaccg gcgccgtcgt aaaattacgt
     1261 gttgcgcgcc gcttatagat agcctccgtg acgtccattg aggaggcttg ctgcggggca
     1321 tatggcaagt tgctcatgca ttgagctccc tgagggcgga cggatttttg agatgaattt
     1381 gccgcgcggc cggaatttcg atgagcgcat cacgctccaa ttgcgacaag gtccgcgaca
     1441 cggtctcaat cgtcagcccg agataatcgg cgatgtcttg ccgcgtcatc gccaatttaa
     1501 cgattctgcc accgtttgat tcgtcgattc catcgatgag aaacgcagca accttctcgg
     1561 ttgcgctccg gcgccccaga agtagcgcgt gttcctgcgc ccgtgccagg ctctgcatcg
     1621 cgtaagagaa aagttgggcg agcaaccctt cgtcatttgc tgcgagcgct tcgagcccgc
     1681 gacgacgata ggaaatcaat gtgcaatcgc ggacggcctc ggctgagaag ccatgcaagc
     1741 cgccgggttc gaaaccgaac acgtccccgg ccttatagaa agcatcaatc tgccggcggc
     1801 cgtcactcag gaatttgcag gtacgcacga cgcctgagac gactttgaag aagagatcgg
     1861 cctcagcacc ttcgctataa atttcccgat cctgcccgaa atgcagcacc gacccagcca
     1921 tgcccagcgc agaacctcgc tccggccgtt cgcccggcgt cttcatccaa gggttgcgtg
     1981 ccaccaccaa gggaggaaac ggcgtggcat gatcaaccga ataggctagc tgcgtcgaca
     2041 tcggaaagct ccatctgcga gaacgttcct tatgtctatc ggccggactg cgggccggat
     2101 attgagttac ctcctagggg attctacgta gagaaacgga cgggatcgaa gatcgcccta
     2161 cgacgaaagg cctaaaacgt cgtggatgtt ctccagcaga gcgctgtccg aaagcggctt
     2221 ttccacgaca tggcgaacgc cggccttcga cgcgcgcttg cgaagcgcgt ccgtcgcggc
     2281 gctggtgatg aggatgacag gaagcgcgat ctgcaaggca tcgaggcgct ccatcacctc
     2341 aaatccgtcg ataaccggca ttcgataatc caaaataaga caacgcgctc gcgacagatc
     2401 aggatgacgc aagagctcca tgccgctgcc gcatgcatgc accacgagtc cctcaagttc
     2461 gagcgcaaac ttgagcgatt cgcgcacggc gaaatcatcg tcgaccacca aaatgatatc
     2521 cgacccacta ttcatttcac ggaaactcca ccggtaaacg aaatgtgagc gccatgtcgc
     2581 accggcgcct tgatgtagcg caagggtcgc caggctcgat catcgtgcag cactttaggg
     2641 gatttgacgt tcagccgccg ggcaatacct ttgcctgcac agccatccgc acaaggccgg
     2701 agaggctgtc cgcacccatt ttcatcatga cgttggcgcg atgcacttca acggtccgcg
     2761 ggctgagctt caggtcataa gcaatcgtct tgttcggata ccctgccaac agaccatcca
     2821 gaacctgccg ctcccgcggt gaaagagatt gtagtcttgt ttgaatctgg acgacctctg
     2881 tctcgtgatg gccaagctca ttgtaacggt cgagcgcgag gcgaatagcg gagagaagca
     2941 gttcatcgtc aaacggcttt tcgataaagt cgacggcgcc agccttcatg gcttccacag
     3001 ccaacggcac atcagcatgg ccggtcatga tgatcaccgg cagatgaacg ccgagagctt
     3061 tcaggcggcg ttgcaattcc agcccatcaa tgcccggcat ccgcacgtca ctgacgatgc
     3121 atcccggttg cagagtacca agagcggcaa gaaatgcgac gcccgattca tagacgcgga
     3181 ccgccaaacc agcggtcgcc agaagaaatg ccaacgactg acgcacgcca tcatcgtcat
     3241 cgatcaaatg cacgaccgaa ttactcatcg ccgaggccct ccccggcgat ggtcttgagc
     3301 gtcagatgaa agatggtccc gccgtccgca ttcggctcgg cccaaaggcg gccgccatgc
     3361 gactcgacaa tcgtccgcga gattgacagc cccacgccca tgccttggcg cttcgtcgtg
     3421 atgaagggct gaaagagttg atgggttatc tccggagcaa taccgggacc ggtatcacgg
     3481 acatcaatcc tcacggtctc attgtcttcc ttatatgtgg agacggtcaa ttcccgccgc
     3541 tcgacctctt gcattgcctc tatggcgttg cgcatcaaat tgagtaagac ctgctgaatc
     3601 tgaatcttgt cggcaaggac aagtgtcgaa cgcgattcaa agagaaatga aacgcgtacc
     3661 cctgtttcct taatacccac aagcgcaagc gcgctcgcct cctcaatcag cctgacgaga
     3721 ttttcgatct gccgctcgct ttcgccgcgc gcgacgaatt cccgcaagcg tcgaatgatc
     3781 tgcccagctc gcagcgcctg gtccgctgcg cgatccatgg cctcacgcgc catcggaatg
     3841 ttatcgctat tgccactctc
//
LOCUS       DASWOG010000579         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_142683,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000579 DASWOG010000000
VERSION     DASWOG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142683"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGB93_11775"
     CDS             <1..677
                     /locus_tag="VGB93_11775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0146798.1"
                     /translation="PGRFTYAFDPGRIAKVIAAAQDADPMQGEIDRAFLNNGKPLWWT
                     PLTYVFLHANWAHVGFNALWLVAFGAPVARRFGPQRFIAFCIGGAVAGALAHLLTHLN
                     DLQPVIGASAVDAATMAAAVRFMFQPDTPLGESGRGSERPPHARPALSLMAVFTDSSA
                     LTFLIFWFVTNFIFGIVSGPGNVGGGAIAWQAHVGGFLFGLLAFTLFDPPYAAPHNED
                     VLPIDD"
     gene            864..1295
                     /locus_tag="VGB93_11780"
     CDS             864..1295
                     /locus_tag="VGB93_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0146799.1"
                     /translation="MTIARILATKGREVETAQPHRTLLEISQILAARRIGAVVIADAY
                     GALLGILSERDIVRALGQGGVHALDDPASSHMTINVITVTEDVTVLDAVELMNTGRFR
                     HMPIMKDGRVDGIISIGDVVKFRLAEIEQEQSAMREYIATV"
     gene            complement(1302..1715)
                     /gene="hisI"
                     /locus_tag="VGB93_11785"
     CDS             complement(1302..1715)
                     /gene="hisI"
                     /locus_tag="VGB93_11785"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0146800.1"
                     /translation="MTPFPPPGGKAELEEGGVFTPRFDADGLIGCITTDASDGAVLMF
                     AHMNAEALRLTLETGIAHYWSRSRKSLWRKGDTSGQQQKVVEIRADCDQDVLLMKVVP
                     RGDGGACHTGRRSCFYRKVALGGGATTLSFDRPKT"
     gene            complement(1723..>2001)
                     /locus_tag="VGB93_11790"
     CDS             complement(1723..>2001)
                     /locus_tag="VGB93_11790"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY0146801.1"
                     /translation="EGVVGLSKLARLVDIYARRLQTQEALTAQIIGAIEKHLRPRGAA
                     VMIEAEHLCMSMRGVMKQGASTVTNQFTGVFRDDPAEQVRFLSLVRGR"
BASE COUNT          360 a          588 c          637 g          416 t
ORIGIN      
        1 tacccggacg cttcacctat gcgttcgacc ctggccggat cgcaaaagtc atcgcggcgg
       61 cgcaagacgc cgacccgatg cagggcgaga ttgatcgcgc ctttctcaac aatggcaagc
      121 cattgtggtg gacgccgctg acttatgttt tcctgcacgc caattgggcg catgtgggat
      181 tcaatgcgct ctggctcgtg gccttcggcg caccggtcgc acggcggttt ggtccgcagc
      241 gttttatcgc cttctgcatc ggcggcgcgg tcgcgggcgc attggcgcat ctcctcacgc
      301 atctgaatga tttgcagccg gtgatcggtg cctcggccgt cgatgcggcg acgatggcgg
      361 cggcggtgcg tttcatgttt cagccggaca caccgctggg cgagagcggg cgcgggtcgg
      421 agcgcccgcc ccatgcgcgg ccggcactat cgctgatggc ggtcttcacc gacagcagcg
      481 cgctgacctt tctcatcttc tggtttgtga cgaattttat tttcggcatc gtatccgggc
      541 cgggcaacgt cggtggcggc gcgattgcct ggcaggcgca tgtcggcgga tttttattcg
      601 gccttttggc atttacgctt ttcgatccgc cttatgccgc gccgcacaat gaagacgttt
      661 taccgattga cgattaaccg gaatgctttt cgcgcgttgc gagaaatgac aagctaagtc
      721 tgaacggcgc acgcgggttg ctgcggcgca agggattcat cttcgcgctt gcagattgat
      781 tcattcgaaa aagccatgca ttcttacggc aatcaatcgc catgaacgcg attgttcacg
      841 aaatgccatg caaggaggtc aagatgacaa tcgcgcgaat cctggcgaca aagggtcgtg
      901 aggtcgaaac cgcgcagccg caccggaccc tgcttgaaat ttcccaaatc cttgccgcgc
      961 gccgtatcgg tgccgtggtc attgcggatg catatggcgc actgctcggc attctgtccg
     1021 agcgcgacat cgtgcgggcg ctcgggcagg gcggtgttca cgcgttggat gacccagcgt
     1081 cgtcgcatat gacaatcaat gtcattacgg taacagaaga cgtaactgtg cttgacgcgg
     1141 ttgaacttat gaacacgggc cgcttccgtc acatgccgat catgaaggat gggcgggtcg
     1201 atggcatcat ctcgattggc gacgtcgtca aattccgact cgctgagatc gagcaggagc
     1261 aatcggcgat gcgcgaatat atcgcgacgg tttagccgag cctaagtttt cggcctgtcg
     1321 aagctgagcg ttgtcgcgcc gccgccgagc gcgaccttgc gatagaagca cgagcggcgg
     1381 cctgtgtggc aagcgccgcc gtcgccgcgg ggcacgactt tcatcaacaa aacgtcctga
     1441 tcgcaatcgg cacggatttc gacgaccttc tgctgttggc cggacgtgtc gcccttgcgc
     1501 cagaggcttt tgcgcgagcg cgaccaataa tgcgcgatgc cggtttcgag cgtcagccgc
     1561 agcgcctcgg cgttcatatg cgcgaacatc aacacggcgc catcgctggc gtcggtggtg
     1621 atgcagccga tgaggccgtc ggcgtcgaag cgcggcgtga agacgccgcc ctcttcgagt
     1681 tcggccttgc cgccgggagg cgggaagggg gtcatggaag tcctatctgc cccggacgag
     1741 ggagaggaac cgcacctgtt cggccggatc atcgcggaag acgccggtaa actgattggt
     1801 gacggtcgaa gcgccctgtt tcatgacgcc gcgcatggac atgcacagat gctcggcctc
     1861 gatcatcacc gccgcgccgc gcgggcggag gtgcttttcg atggcgccga taatctgcgc
     1921 cgtgagcgct tcctgtgtct gcaggcggcg cgcatagata tcgacgagcc gggccagttt
     1981 cgacaggccg acgacgccct c
//
LOCUS       DASWOG010000580         6894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_68789,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000580 DASWOG010000000
VERSION     DASWOG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68789"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VGB93_11795"
     CDS             <1..1091
                     /locus_tag="VGB93_11795"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY0146802.1"
                     /translation="EYVARLGGDEFAVLQFPFKTSEDIDRLAEKLLNCFDEPFEIEGR
                     KITISASIGIARAPIDSDNFTDLLRRADVALYRAKSVGRATYCPFNEEMGRQLLIRRE
                     IEGDLREAIENDELVPYFQPIVDLATGRVIAFEALARWILPAGSFVPPNLFIPLAEEM
                     GIIGELGEQILRRACIEAAAWPQDIKVAVNLSSLQLGRHGFIQEIEHALADSGLAPER
                     LELEITETVPLIENAPMREILHGLKRRGISIALDDFGTGYSSLSYLRSFPFDRLKIDR
                     SFVGEIHEHEETMAVIKAMLDLARSLDVRTTAEGIETEQQRIWLLARGCSEGQGYLFE
                     RPMPAAEVADFLRAHPATGTPAFRRRNA"
     gene            complement(1097..2383)
                     /gene="eno"
                     /locus_tag="VGB93_11800"
     CDS             complement(1097..2383)
                     /gene="eno"
                     /locus_tag="VGB93_11800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0146803.1"
                     /translation="MTAIVDIAGREILDSRGNPTVEVDVTLEDGSFGRAAVPSGASTG
                     THEAVELRDGDKGRFGGKGVLKAVESVNRDIFDALAGMDAEDQVAIDEAMIVLDGTPN
                     KSNLGANAILGVSLAVAKAAADVSGLPLFRYVGGTQARVLPVPMMNILNGGVHADNPI
                     DFQEFMILPYAAPSLTEAVRWGAEVFHVLKSALKKAGLNTNVGDEGGFAPNLPSAEAA
                     LDFCLKGIEGAGFKPGKDIALGLDCASTEFFRDGAYRYEGEGKTLKIEEQAQYLAKLA
                     ANYPIATIEDGMSEEDWEGWKILTDLLGKKVQLVGDDIFVTNVERLAKGIADGVGNSV
                     LIKVNQIGSLTETMAAVDMAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSL
                     ARSDRAAKYNQLIRIEEELGAQARYAGRAALKAWAA"
     gene            complement(2380..3531)
                     /locus_tag="VGB93_11805"
     CDS             complement(2380..3531)
                     /locus_tag="VGB93_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0146804.1"
                     /translation="MEYRQLGASGFKVPVLSFGTGTFAGKGELFAAWGSSDVKEAIRL
                     IDICLDAGLTLFDTADVYSAGASEEVLGGAIKGRRDKVLISTKATFRTGDTPNDLGSS
                     RFHLTQTINASLKRLQTDYIDLFQLHGFDALTPIEEVLSTLDDFVRAGKIRYIGCSNF
                     SGWHLMKSLAIADRYNLPRHVAHQAYYSLIGREYEWELMPLALDQNVGAVVWSPLGWG
                     RLTGKIRRGQPLPETSRLHKTADMGPPVDDEYLYKVVDALDEIAQETGKTIPQIALNW
                     LLQRPTVATVIIGARNEEQLKQNLGAVGWSLTPEQVKKLDAASERPLTYPYWHQRLFA
                     ERNPPPVYLLSSRASYRPFGVPAESPPFFGSMFVVEPAANEETRRKDAS"
     gene            complement(3558..3644)
                     /locus_tag="VGB93_11810"
     CDS             complement(3558..3644)
                     /locus_tag="VGB93_11810"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146805.1"
                     /translation="MRREDVTINLVEVKKENWSFGNGEAPYA"
     gene            complement(3626..3937)
                     /locus_tag="VGB93_11815"
     CDS             complement(3626..3937)
                     /locus_tag="VGB93_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEY0146806.1"
                     /translation="MPILRAEMPRGKSPEFKLGIGDVVYAAMRATLNVPEGDRFQIFT
                     EHAPEDLSIDPTYLGIRRSPEALILQITLNEGRTTDQKHAFYKAIADGLIASSVCGAR
                     T"
     gene            complement(3968..4894)
                     /locus_tag="VGB93_11820"
     CDS             complement(3968..4894)
                     /locus_tag="VGB93_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEY0146807.1"
                     /translation="MRILVIGAGALGGYFGGRLLAAGRDVTFLVRPRRAAQLAEHGLE
                     IISVKGGLHLAKPPTVTADKLNQHYDLVLLSCKAFDLTDAIKSFAPAVGPQTMILPVL
                     NGMLHLDILASHFGAEHVLGGLSKISATLDAEGRIHHLSSFHSISFGEQNGARTERIE
                     ALAEMLRSSGFDAPLSENILAEMWNKWVFIAAAASLGCLMRAQVGDIVATGAASYGKI
                     LLNECAAIAARLNFPQDAQGLQFGEKMLTEAGSTFKPSMLRDIERGQQTEAHHIVSDL
                     LRRSGDLPTPILQIADAHLKVYEARRAREAGA"
     gene            5131..5469
                     /locus_tag="VGB93_11825"
     CDS             5131..5469
                     /locus_tag="VGB93_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0146808.1"
                     /translation="MRTTQFSILTRLHESGPMSIHALAEAMVMDRTTLGRNILPLERD
                     GLIVVAQSDRDKRSKELTLTATGRERLKRAVDGWLEAQSQFEKAFGAKRASDLREILH
                     DVAAADLGDA"
     gene            5625..6077
                     /gene="queF"
                     /locus_tag="VGB93_11830"
     CDS             5625..6077
                     /gene="queF"
                     /locus_tag="VGB93_11830"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY0146809.1"
                     /translation="MSKKPAALGHQTALPASPDDARLDRVPNPHPDADYVARFSAPEF
                     TSLCPVTGQPDFAHLVIDYVPDRWLVESKSLKLYLGSFRNHGAFHEDCTLRIGKDLVG
                     LLKPRWLRIGGYFYPRGGMPIDVFWQSGKLPAGVWVPDQGVAPYRGRG"
     gene            complement(6093..6425)
                     /locus_tag="VGB93_11835"
     CDS             complement(6093..6425)
                     /locus_tag="VGB93_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146810.1"
                     /translation="MKILSTVLAMSVLAVAWGPAQAATHHHVHYVHVVHHYHHHYYAG
                     GVSYYGAPAGYYPPPPGYYPPPSGYYPAPGIYAPGYFYATPEEPLGNRGIGKALIDSE
                     WDPPFSGR"
     gene            complement(6522..>6894)
                     /locus_tag="VGB93_11840"
     CDS             complement(6522..>6894)
                     /locus_tag="VGB93_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146811.1"
                     /translation="AETIALLMDRADELSAFERFHFAGHQLARGEMAAAIRRATDNPR
                     LRVWPFPWFLVVGLQPFVRLFREMAEMRYVWRESISIDGAKLRAFLGNALPTAPLDKA
                     VCETLIGLGCLPGAGEKAGAR"
BASE COUNT         1400 a         2087 c         2030 g         1377 t
ORIGIN      
        1 atgaatatgt cgctcggctt ggcggtgacg aattcgccgt attgcagttt ccgttcaaaa
       61 cgtccgaaga tatagatcgc ctcgcggaaa agcttctcaa ttgctttgac gagccattcg
      121 agattgaagg ccgcaagatc acaatttccg caagtatcgg catcgcgcgc gccccaatcg
      181 acagcgacaa tttcaccgat ctgctccggc gcgcggatgt cgctctctac cgcgccaaaa
      241 gtgtcggccg cgcgacctat tgcccgttca atgaagagat gggccgacag cttctcattc
      301 ggcgggagat cgaaggcgat ctgcgcgaag caatcgagaa cgacgagctt gtgccgtatt
      361 tccagcctat cgtcgatctt gctaccggaa gggtaatcgc gttcgaggcg ttggctcgct
      421 ggatcttgcc cgcgggcagc tttgtcccgc cgaatctttt cattccgctc gctgaggaga
      481 tgggcatcat cggcgaacta ggcgagcaga tactacgtcg cgcctgcatc gaggcggcgg
      541 catggccgca ggatatcaaa gtcgccgtca acctgtcctc gttacagctc ggtcgccatg
      601 gcttcattca agaaattgaa catgctcttg ccgacagcgg cctcgcgccc gaacggctcg
      661 aactcgagat tacggaaacc gttccgctga tcgaaaacgc gccgatgcgc gaaatattgc
      721 acggtctcaa acgacgcggc atcagcatcg cgctcgacga tttcggcacc ggctattcgt
      781 cattgtccta tctgcgctcg ttcccgtttg accgtttgaa gattgatcgg tccttcgtgg
      841 gcgaaattca cgaacacgag gagacgatgg ccgtcatcaa agcgatgctc gacctcgcgc
      901 gcagcctcga cgttcgcacg acggccgaag gaatcgagac cgagcagcaa cgcatctggc
      961 tgctggcgcg cggctgctcg gagggacaag gctatttgtt cgagcggccg atgccggcgg
     1021 ccgaggtcgc cgactttttg cgcgcgcacc cggctaccgg cacgcccgca ttccgacgcc
     1081 ggaatgcctg aacgttctag gccgcccagg ctttgagcgc agcgcggcca gcatagcgcg
     1141 cctgggcacc aagctcttct tcaatgcgga tgagctggtt atatttcgcc gcccgatcgg
     1201 agcgcgcgag cgagcctgtc ttgatctgcc cgcaattggt ggcgactgcg agatcagcga
     1261 tcgtcgaatc ttccgtctcg cccgagcgat gcgacatgac cgccgtgtag ccagcccgct
     1321 gcgccatatc gacggcggcc atggtctcgg tcaacgaacc gatctggttg accttgatca
     1381 ggaccgaatt gccgacgcca tccgcgatgc ccttagccag gcgctcgaca ttggtgacga
     1441 aaatatcgtc gccgacgagt tggaccttct tgccgagaag atcggtgagg attttccagc
     1501 cttcccaatc ctcctcagac atgccgtcct cgatcgtcgc gatcggataa ttggcagcga
     1561 gcttggcgag atattgcgcc tgctcttcga tcttgagggt cttgccctcg ccttcgtagc
     1621 gataggcacc gtcacggaag aattcggtcg aagcgcaatc gagcccaagt gcaatgtcct
     1681 tgcctggctt gaacccggcg ccttcgatcc ctttgaggca gaaatcgagc gcggcttcag
     1741 ccgacggaag gttcggcgcg aagccgccct catcgccgac attggtatta aggccggcct
     1801 ttttcaacgc actcttgaga acatggaaga cttcggcgcc ccagcgcacg gcttccgtta
     1861 acgacggcgc ggcgtaaggc aggatcatga attcctgaaa gtcgatcgga ttgtcggcgt
     1921 gaacgccgcc attcaagatg ttcatcatcg gcaccgggag gacccgcgcc tgcgtaccgc
     1981 caacatagcg aaacagcggc agcccgctga catcggcggc ggccttcgcg acggcaagcg
     2041 atacgccgag gatggcattg gcgccaaggt tcgatttgtt cggtgtccca tcgaggacga
     2101 tcatcgcctc gtcgatggcg acctgatctt cggcatccat gccggcgagc gcgtcgaaaa
     2161 tatcgcgatt gacggattcg accgccttca ggacaccttt gccgccgaag cgtcccttgt
     2221 cgccgtcacg caattcgacg gcctcatgcg ttccggtcga ggcacccgat gggaccgccg
     2281 cacgcccgaa agacccatcc tccaaggtta cgtcaacttc gaccgtcgga ttgccgcggc
     2341 tatccagaat ttcgcggcca gcaatatcga caatggcggt catgatgcgt cctttcggcg
     2401 ggtttcctcg tttgcggcgg gttctacgac gaacatgctg ccgaaaaaag ggggcgactc
     2461 ggccgggacg ccgaaaggac gatatgacgc tcttgatgac agcaaataga ccgggggtgg
     2521 attacgctcg gcgaaaagcc gctgatgcca atagggatat gtcagcggcc gctcgcttgc
     2581 ggcgtcgagc tttttcactt gttctggcgt cagcgaccag ccgaccgcgc caaggttctg
     2641 tttgagctgc tcctcgttgc gggcgccgat gataacggtc gcgacggtcg ggcgctgcaa
     2701 tagccagttg agagcgatct gcgggattgt cttgccggtc tcctgggcaa tttcgtccag
     2761 cgcatcgaca actttataga gatattcatc gtcgaccggt ggtcccatgt cggccgtttt
     2821 atgcagacgg ctcgtttccg gcaggggttg gccgcggcga atctttcctg tgaggcgccc
     2881 ccaaccgagt gggctccaga caaccgcgcc gacgttctgg tcgagcgcca ggggcatcag
     2941 ctcccattcg tattcgcggc cgatgagcga ataatacgcc tggtgcgcga catggcgcgg
     3001 caagttgtag cgatcggcga tcgccagcga tttcatcaga tgccaaccgg agaaattcga
     3061 acagccgata tagcggatct tcccggcacg gacgaaatcg tcgagcgtcg agagcacttc
     3121 ctcaatcggc gtcagcgcgt cgaagccatg caattggaaa agatcgatat aatcggtctg
     3181 caggcgcttc agcgatgcgt tgatcgtctg tgtcaggtga aagcgcgaag agccgaggtc
     3241 attgggcgtg tcgcctgtgc gaaaagtcgc cttggtcgag atcagcacct tgtcgcggcg
     3301 gcccttgatg gcgccaccaa gcacctcctc agaggcacca gccgagtaaa catccgccgt
     3361 gtcaaagagc gtgaggcctg catcaaggca gatgtcgatc aggcgtatcg cctccttcac
     3421 gtcgctcgag ccccaggctg cgaacaattc gcctttgccg gcgaacgtgc cggtgccgaa
     3481 actcaggacg gggactttga accccgaagc acctaattgc cgatattcca tatttccctc
     3541 atccgcttgg atgtcgctca ggcatatggg gcttcgccat tgccgaatga ccaattctct
     3601 ttcttcactt cgacgagatt gatcgtcacg tcctcgcgcc gcacaccgag ctggctataa
     3661 ggccatccgc aatggcctta tagaaggcgt gcttctgatc ggtcgtgcgc ccttcgttca
     3721 gcgtgatttg caggatcagc gcctcgggcg aacgcctgat gccaagatag gtcgggtcga
     3781 tcgaaagatc ttcgggcgcg tgttcggtga aaatctgaaa acgatcgccc tcgggcacgt
     3841 tgagcgtcgc acgcattgct gcatacacga cgtcaccgat tccaagctta aactcgggtg
     3901 acttgccgcg cggcatttca gcacgaagga taggcatgat ctttcctcac tgcgttgaat
     3961 ctatgcgtca ggcgccggct tcgcgggcac ggcgcgcctc gtagaccttg agatgcgcgt
     4021 cggcgatctg caggatcggc gtcggcagat cgccggaacg gcgcaaaaga tcgctgacaa
     4081 tgtgatgcgc ctccgtctgc tggccgcgct cgatgtcgcg caacattgac ggtttgaagg
     4141 tcgagcccgc ttcggtcagc atcttctcgc cgaattgtaa gccttgtgcg tcctgcggaa
     4201 aattaagccg tgcggcaatc gccgcgcatt cgttcaacag aatcttgcca taagaggcgg
     4261 cgccagtggc aacgatgtcg ccgacctgag cccgcatcag gcagccgagg ctcgccgcgg
     4321 ccgcgatgaa gacccatttg ttccacattt cagcaaggat attttcgctc aggggtgcgt
     4381 cgaagccgct ggaacgcaac atctcagcca aggcctcgat gcgttccgtg cgcgcgccgt
     4441 tctgctcgcc gaagctgatg gaatggaagc tgctcagatg atggatgcgg ccctcggcgt
     4501 cgagcgtcgc ggaaatcttg cttaggccgc cgagaacgtg ctcggcgccg aaatgcgagg
     4561 cgagaatatc gaggtgcagc ataccgttga gcaccggcag gatcatcgtc tgggggccga
     4621 cagccggggc aaaggacttg atcgcatcgg tgaggtcgaa agccttgcag ctcaggagca
     4681 cgagatcata gtgctgattg agcttatcgg ccgtcacggt cggcggcttg gcgagatgca
     4741 atccgccttt gacgctgatg atttcaaggc cgtgttcggc aagctgcgcc gcacgacgcg
     4801 gccggacaag aaacgtaaca tcacgccccg ccgcgagaag gcggccgcca aaatagccgc
     4861 cgagcgctcc cgcaccaatc acaagaatcc gcataaactg tctcctgaac cgccgtcttt
     4921 aaacattttt ggcgcgcttt agctaaccag cgccgatcaa gatcagttga catgcgcgca
     4981 tatgctctta tctcctgata aggcaaggtt tttcggaaat ttggagcggc cctgggcaag
     5041 aacacaccat cggattgcaa ttgcctggcg gcgcgacagg ccgcgcgccg catcacgcaa
     5101 ttctatgacc gctttctggc gcccgcggcg ttgcggacaa cgcagttttc aattctgacg
     5161 cggcttcatg aatccggccc catgagcatc cacgctctcg ccgaagcgat ggtcatggac
     5221 cgcacgacgc tcggccgtaa cattttgccg ctcgagcgcg atgggctgat cgttgtcgcc
     5281 caaagcgaca gggacaagcg cagcaaggaa ttgacactca cggcgaccgg gcgcgaaagg
     5341 ctgaaacgcg cggtggacgg ctggctggaa gcccaatccc agttcgaaaa agcctttggc
     5401 gccaaacgcg ccagcgatct tcgcgaaatc cttcatgatg tcgcggcggc cgatctcggc
     5461 gatgcctaga gcatcccgct ttcagataga atcatccaaa agcggacaag atgctctaga
     5521 ttcaaagatg tagagcatgc ttttgtgcga aaaatcgcgc acacttttgc gcagcatgct
     5581 ctagacgcaa tggccgtttg ccgcccgcaa cgttataagc acccatgagc aaaaagcccg
     5641 ccgccctcgg ccaccagacc gccctgcccg cctcacccga cgacgcccgg ctcgaccgcg
     5701 tgcccaatcc gcatcccgac gcggattatg tcgcgcggtt ctcggcgccg gaattcactt
     5761 cgctttgccc ggtgacaggt cagccggatt tcgcccatct cgtcatcgat tatgtgccag
     5821 accgctggct cgtcgaatcg aaatcgttga agctctatct cggctcgttc cgcaatcacg
     5881 gcgcttttca cgaggattgc accctgcgga tcggcaagga tctcgtcggg ctgctcaaac
     5941 cgcggtggct gcgtatcggc ggctatttct atccgcgcgg tggtatgccg atcgacgttt
     6001 tctggcagag cggaaagttg cccgccggcg tttgggtgcc ggatcagggc gtcgcgccct
     6061 atcgcgggcg cggctagagc gcgtggtagc gtttagcgtc ccgagaacgg cggatcccat
     6121 tcgctgtcga tcagcgcctt gccgatgccg cgatttccca aaggttcttc cggcgtggcg
     6181 tagaagtaac ccggagcgta aatgccgggt gcaggataat agccggacgg cggcgggtaa
     6241 tagccaggcg gcggcggata ataaccggcc ggcgctccgt aatagctcac gccgcctgca
     6301 taataatgat gatggtaatg atgcaccacg tgaacgtagt gaacgtggtg atgggttgcg
     6361 gcctgcgcag gtccccacgc gacggccagc accgacattg cgagaacggt agataagatt
     6421 ttcatcaaat gacctttctg cgtgccgcga tccgtgccga ccgggttgtt ttacatgtag
     6481 gcacgcaaaa ccgcaaaaac agccgggtgc gttcgcaaag cctagcgtgc gcctgccttc
     6541 tcgcctgctc ccggcagaca tcccaacccg atcagcgtct cgcacacggc tttgtcgagc
     6601 ggcgcggtgg gaagcgcatt accaagaaaa gcccggagtt tcgcgccgtc gatcgagatc
     6661 gattcgcgcc agacgtagcg catctcggcc atttcgcgga acagccgcac gaaaggctgc
     6721 agaccaacaa ccaggaacca gggaaacggc cagacgcgca ggcgcggatt atccgtagcc
     6781 cggcggatcg ccgccgccat ttcgccccgc gccaattgat ggccagcgaa atgaaaccgt
     6841 tcgaacgcgc ttagctcatc cgcgcggtcc ataagaagcg caatcgtctc cgcc
//
LOCUS       DASWOG010000581         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_215331,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000581 DASWOG010000000
VERSION     DASWOG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215331"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VGB93_11845"
     CDS             <1..173
                     /locus_tag="VGB93_11845"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY0146812.1"
                     /translation="VPGSVETSVDNSYGMERVEAHCSRCGSHLGHVFPDGPPPTHLRY
                     CINGVAMNFHPE"
     gene            complement(250..477)
                     /locus_tag="VGB93_11850"
     CDS             complement(250..477)
                     /locus_tag="VGB93_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146813.1"
                     /translation="MYIIARNIKGPAKTRNLRCEALMVDKASFTVWPPSTEDGWRRAY
                     KFRDKLMAEVVRLELDPMGEKLKVIEAVYIP"
     gene            complement(569..886)
                     /locus_tag="VGB93_11855"
     CDS             complement(569..886)
                     /locus_tag="VGB93_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0146814.1"
                     /translation="MDEMKSPREAARPFTPKLTALVESPLYDKVWEDPELSKRDRSLV
                     TVASLIALGNTDELPAHLRRAIDNGVTKTELSALITHLAFYVGFPAAITASHTAHKVL
                     GPI"
     gene            complement(894..2693)
                     /gene="lepA"
                     /locus_tag="VGB93_11860"
     CDS             complement(894..2693)
                     /gene="lepA"
                     /locus_tag="VGB93_11860"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0146815.1"
                     /translation="MTKLENIRNFSIVAHIDHGKSTLADRLIQTTGTVAARDMVEQVL
                     DSMDIERERGITIKAQTVRLQYNAKDGQSYILNLMDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYHALDAGHEIVPVLNKIDLPAAEPERIKQQIEDVIGLDASN
                     AVLISAKTGLGIEDVLEAIVKRLPPPKGDAKSPLKALLVDSWYDAYLGVVVLVRVFDG
                     VMKRHQKIKMMGTDAHYVIDRIGVFRPKMQDVEELGPGEIGFITAQIKQVADTRIGDT
                     ITDERHSVAEALPGFKPAQPVVFCGLFPVDAADFEDLRSAMGRLRLNDASFSYEMESS
                     AALGFGFRCGFLGLLHHEIIQERLEREFNLDLIATAPSVVYQLVMRSGETISLHNPAD
                     MPDPSRIERIEEPWIRATILTPDDYLGAVLKLCQERRGVQVDLNYVGARAMAVYDLPL
                     NEVVFDFYDRLKSISKGYASFDYTITDYREGDLVKMSILVNAEPVDALSMLVHRDRAE
                     MRGRVMVEKLKELIPPHMFQIPIQAAIGGKIIARETVRALRKDVTAKCYGGDATRKRK
                     LLEKQKAGKKKMRQFGKVEIPQEAFIAALKMDS"
     gene            2915..3619
                     /locus_tag="VGB93_11865"
     CDS             2915..3619
                     /locus_tag="VGB93_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0146816.1"
                     /translation="MQPTRLSRDDRRQQILECALPLFARHGFGGTTTRRIADAAGISE
                     ALLFKHFPTKSAIYAAILADICTADPGLARLRAEPPSTRTLALIVRGMTHYFISAADG
                     SDAEGNQKLRLTFSSYLEDGEFAKILFDKVEQVIAPIFINSLDHAIAVGDALPGGANS
                     RNLFWFAHHTLCTLAATQMPLLPSLDYPPPVELEREVCIYILRGIGLTDAAIEMHAAL
                     DIPFESFEASVLEFAR"
     gene            3616..>3935
                     /locus_tag="VGB93_11870"
     CDS             3616..>3935
                     /locus_tag="VGB93_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEY0146817.1"
                     /translation="MTLSAETEVSARTAYRKPKTDKPVRTKLWFSIMVVLMALLVGVF
                     WGFNQFRNHMIAQFFANMKMPPATVSVAGVKSEVLPNLLTGIGDVVAVHQVNVTSEVS
                     GRVV"
BASE COUNT          729 a         1177 c         1194 g          835 t
ORIGIN      
        1 cggtgcccgg ctcggtcgag accagcgtgg acaacagcta cggcatggag cgcgtcgaag
       61 cgcattgtag ccgctgcggc agccatctcg gacacgtatt tcctgacggg ccgccgccga
      121 cgcacctgcg ctattgcatc aacggcgtgg cgatgaattt ccacccggaa taggccgtca
      181 tggccgggca tagcccgttg aagacgggcg ttctaacgaa cgccttatgt cccggccatc
      241 acgccgcttc tatgggatat agaccgcctc gatcaccttc agcttctccc ccatcggatc
      301 gagttcgagg cgcacgactt cggccatgag cttgtcgcgg aatttatagg cgcggcgcca
      361 gccgtcctcg gtcgagggcg gccaaactgt gaagcttgcc ttgtcgacca tcagcgcctc
      421 gcagcggagg ttccgagtct tggcggggcc tttgatgttg cgggcgatga tatacatggc
      481 gcctcgatcc gcagcgcatc tgattgaggc agtttatcgc cgtggctgcg tttgtcacga
      541 agcgattttc gtcgatggcg gtgttcgctt aaatcggtcc cagcacttta tgcgcggtat
      601 gcgaggccgt gatcgcagcc ggaaatccca cataaaacgc gagatgtgta atcagggcgc
      661 tgagttccgt cttggtgacg ccattgtcga tggcgcggcg caaatgggcc ggtagctcat
      721 cggtgttgcc aagggcaata agcgaggcga cggtcacaag gctgcggtcg cgtttggaaa
      781 gttcaggatc ttcccaaacc ttatcgtaga gcgggctctc aaccagcgct gtgagtttcg
      841 gtgtgaaagg gcgtgcggct tcacgcgggc ttttcatctc gtccataacg tcctcacgaa
      901 tccattttca gcgcggcgat aaaggcttcc tgcggaattt ccactttccc gaactggcgc
      961 atctttttct tgccggcctt ttgcttttcg agaagcttgc gcttgcgcgt cgcgtcgccg
     1021 ccgtagcatt tcgccgtcac gtccttgcgc aaggcgcgca ccgtctcgcg ggcgatgatc
     1081 ttgccgccga tcgctgcctg aatcgggatc tggaacatgt gcggcgggat cagctctttc
     1141 agcttttcca ccatgacgcg gccgcgcatc tccgcccggt cgcgatgcac tagcatggag
     1201 agcgcatcga ccggctcggc attgacgagg atcgacattt tgacgagatc gccctcgcga
     1261 taatcggtga tggtgtaatc gaagctggca tagcccttcg agatcgactt gagccggtcg
     1321 tagaaatcga agacgacttc gtttagcggc aagtcgtaga ctgccatcgc tcgcgccccg
     1381 acatagttca aatctacctg gacgccgcgc cgctcctgac agagcttcag cacggcgccg
     1441 agataatcgt ccggcgtcag gatcgtcgcg cggatccacg gctcttcgat gcgctcgatt
     1501 cgggacgggt ccggcatatc ggcgggatta tgcagcgata tcgtttcgcc cgaccgcatc
     1561 acgagctgat agacgaccga aggcgcagtc gcgatcaggt cgaggttgaa ttcgcgctcc
     1621 agccgttcct gaataatttc gtgatgcagc aggccaagga agccgcagcg gaagccgaag
     1681 ccgagcgccg ccgagctctc catttcatag gaaaagctcg cgtcgttgag gcgcagcctg
     1741 cccatcgccg agcgaagatc ctcaaaatcc gccgcgtcca ccggaaacag gccgcagaag
     1801 acgacgggct gggcgggttt gaagccgggc agggcctcgg cgacggaatg gcgctcgtct
     1861 gtgatcgtgt cgccgatgcg ggtatctgcg acctgcttga tctgcgcggt gatgaagccg
     1921 atctcgccgg ggccgagttc ctcgacatcc tgcatcttcg gccgaaagac gccgatacgg
     1981 tcgatcacat aatgggcgtc ggtgcccatc atcttgatct tctgatgcct cttcatcacg
     2041 ccgtcgaaaa cgcgcaccag cacgacaacg ccgagataag catcgtacca ggaatcgacg
     2101 agaagtgcct tcaacggcga tttggcgtca cccttcggcg gcggcaggcg cttcacgatc
     2161 gcctctagca catcctcgat gccgaggccg gtcttggccg agatgagcac ggcattggag
     2221 gcatcgaggc cgatgacatc ctcgatctgc tgcttgatgc gctcgggttc tgcggccggc
     2281 agatcgatct tgttcagaac gggcacgatc tcgtggccgg cgtcgagggc gtgatagacg
     2341 ttggcgagcg tctgcgcctc gacgccctgg ctcgcgtcga cgaccagcag cgagccttcg
     2401 caggccgcca gcgagcgcga gacttcatag gcgaaatcga cgtggccggg cgtgtccatt
     2461 agattgagga tatagctctg cccatctttg gcattgtatt ggaggcgcac cgtctgcgcc
     2521 ttgatggtga tgccgcgctc gcgctcgata tccatcgagt ccagcacctg ttccaccata
     2581 tcccgcgcgg ccactgtgcc ggtcgtttgg atcagccggt cggcgagggt cgatttgccg
     2641 tggtcgatat gcgcgacgat ggagaaattg cggatatttt cgagcttggt catgcgtcgc
     2701 gggatagcag gcgccccacg cgaaagaaag gcgtgcgttg cacgatcgcg gggattgtcc
     2761 cggcaacatt tggggctatc ccgctggccg gcctgcactt ttattgttcg gaacgtgggg
     2821 cgccaggaaa attcggttcg cgtcgccgcc gcggagggga gaaaaattta cggcccagct
     2881 tgacaggccg gtaagtgagt ggtcacttac aagcatgcaa ccgacccggc tctcgcgcga
     2941 cgaccgtcgc cagcagattc ttgagtgcgc gctgccgctc ttcgcccgtc acggttttgg
     3001 cgggacgacg acccggcgga ttgccgatgc cgcggggatt tcggaagccc tgcttttcaa
     3061 gcacttcccg actaagtcgg cgatctatgc ggcgatcctc gcggacatct gtaccgccga
     3121 tccgggtctt gcccggctgc gggccgagcc gccctccacg cgcacgctgg cgttgatcgt
     3181 gcgtggcatg acgcattatt tcatatctgc cgcggacggt tcggatgctg aaggtaatca
     3241 gaagctgcgg ctgaccttct cgagctatct cgaggacggt gaattcgcca aaatattgtt
     3301 cgacaaggtc gaacaggtca tcgcgccgat tttcatcaat tcgctcgatc atgccatcgc
     3361 ggtcggtgac gcgctgcccg gaggcgctaa ttcgcgcaat ctcttctggt tcgcgcatca
     3421 cacgctctgc acgctcgcgg caacgcaaat gccgctgctg ccctcgctcg actatccgcc
     3481 acccgtcgag ctggaacgtg aagtctgcat ctacatcctg cgcgggatcg gcctcaccga
     3541 tgccgcgatt gagatgcatg ccgctctcga tatccccttt gaaagtttcg aagcctccgt
     3601 attggaattt gccagatgac gctctctgct gaaaccgaag tctccgccag aacggcctat
     3661 cgcaagccca agaccgacaa gccggtgcgc accaagctct ggttctcgat catggtggtg
     3721 ctgatggcgc tgctcgtcgg agtgttctgg ggctttaatc agtttcgcaa tcatatgatt
     3781 gcgcaattct tcgccaatat gaaaatgcca ccggcgacgg tcagcgtcgc gggtgtcaaa
     3841 agcgaggttc tgcccaatct cctgaccggc atcggcgatg tcgtcgcggt gcatcaggtc
     3901 aatgtgacga gcgaagtttc cggccgcgtc gtaaa
//
LOCUS       DASWOG010000582         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_234877,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000582 DASWOG010000000
VERSION     DASWOG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234877"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VGB93_11875"
     CDS             <1..755
                     /locus_tag="VGB93_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0146818.1"
                     /translation="TWPDLPLQVRLNAPVGRMDVNAAIARVEAHLAQHPQDGLGYQVL
                     AQALLITGRVGDAVNAAQTAVRLRPNNADTLTVYGETLVVASNGIVTEDARRQFEKAI
                     SSDPHEAKAMFFLGLAASQENDVVTARKYWGPLLASAPPNAPWRADLVARLGQLDKGL
                     PASGQAALIAAMSPAQQQATIQSMVDGLADRLAQNGNDIDGWVRLVRAYKVLNEIDKA
                     RAALKTARHDFDGDAAATARIDALAHELGLES"
     gene            752..1201
                     /gene="ccmE"
                     /locus_tag="VGB93_11880"
     CDS             752..1201
                     /gene="ccmE"
                     /locus_tag="VGB93_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY0146819.1"
                     /translation="MTRKKKRATLIIAAMAVLGTALGLVLYALRDNIVFFYGPSEVVA
                     KDLPPGTRVRIGGLVKKGSVVHEGQDVRFDVTDGKHEIAVTYDGALPDLFREGQGVVA
                     EGVLAAGGLDASEVLAKHDERYMPKEVVDALKKSGRWQENADMGSSQ"
     gene            1198..3195
                     /locus_tag="VGB93_11885"
     CDS             1198..3195
                     /locus_tag="VGB93_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY0146820.1"
                     /translation="MIVETGHFALILALALACLNAVVPLWGVATRDSRLMGIAPTVAL
                     AGFGFVALSYAALTYAHITSDFSLVNVYENSASAKPLIYKITGVWGNHEGSMLLWVLI
                     LAVFAAAVALSARAMPQDLHAAVLAVQSWISAAFLLFILLTSDPFARMANPPIEGQDL
                     NPLLQDPGLAIHPPLLYLGYVGLSITFAFAAGALICGRIDGVWARLVRPWTLIAWIFL
                     TLGIAMGSYWAYYTLGWGGFWFWDPVENASLMPWLAATALLHSATVMEKREALKVWTI
                     FLAILAFSLSLIGTFIVRSGVLTSVHSFANDPQRGEFILAILVFFIGGALLLFALRAN
                     TLKPGGFFAPVSREGALVLNNLVLTTCCATVFVGTLYPLALQSFTGEKISVGGPFFNA
                     TVVPLFLPLVLLMPVGQNLAWKRGDFLGAAQRLYFALLCGLAMVLVLAGWHHNGPLTS
                     IIVSGLGVYVIVGAFAEIVSRSFGRIALVKGGIAQVWRHATGLPLSTYGTALAHAGIG
                     VSLLGLAGTSWGAEKVLTMKPGDSAIVGPYQLTLTSLVSETGPNFTAKVGHVVIRSGE
                     REIAAINPAVRFYPTRQMSRAEAGIKTLNLGQVYVGISDGVKPGTVDARLYWKPLVTL
                     IWIGALVMGFGGLLSLSDRRLRIGVAHRFRKFAGAQQAPAE"
     gene            3192..3662
                     /locus_tag="VGB93_11890"
     CDS             3192..3662
                     /locus_tag="VGB93_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007825878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY0146821.1"
                     /translation="MKWLLRLIVLAVLTLGPLLAHAVQPAEMLSDPQLEARARAISAG
                     LRCLVCQNESIDESNATLAHDIRVLVRERLLAGDSDEQIRNFLVSRYGNFILLKPPFE
                     PGTYLLWLTPLILLLAGGGAIYLNTRRTPPAAPANLTSTEATKLAQIVGHTDDL"
BASE COUNT          651 a         1229 c         1233 g          875 t
ORIGIN      
        1 cgacgtggcc ggatttgccg ttgcaggtgc ggctcaacgc tccagtcgga cgcatggacg
       61 tcaatgccgc catcgcccgg gttgaagccc atctggcgca gcacccgcag gatggtcttg
      121 gctatcaggt gctggcccaa gctctcttga tcaccgggcg cgtgggcgat gcagtcaacg
      181 ccgcgcagac ggcggttcgt ctgcgcccca acaatgccga tacgctgact gtctatggcg
      241 aaacgctcgt cgtcgcatcg aatggcatcg tgacggagga cgcgcgtcgt cagttcgaaa
      301 aggcgatctc ctccgatccg cacgaggcca aggccatgtt tttcctcggc ctcgcggcgt
      361 cgcaggagaa cgatgttgtg acggcgcgca aatattgggg accgctttta gcgtcggcgc
      421 cgcccaacgc accttggcgg gccgatctcg tcgcgcggct cgggcaactt gataagggtc
      481 tgcccgcgtc cggtcaggcg gcactgatcg ccgccatgtc tccggcgcag caacaggcga
      541 cgatccagtc gatggtcgac gggctggcgg accgccttgc gcagaatggt aacgatattg
      601 acggttgggt gcgtctcgtt cgcgcctata aggttctgaa tgaaatcgac aaggcgcgcg
      661 cggcgttgaa aaccgcccgg cacgatttcg atggcgacgc cgctgcgacc gcgcggatcg
      721 atgcgctggc gcacgagctt ggacttgaat catgaccagg aaaaagaagc gcgcgacgct
      781 gattattgcg gcgatggccg tgctaggcac ggccctgggc ctcgtgctct atgcgctgcg
      841 cgacaacatc gttttctttt acgggccgag cgaagtcgtc gcgaaagacc tgccgccggg
      901 cacacgggtg cggatcggcg gtctggtcaa aaagggttct gtcgtccacg agggccagga
      961 tgtgcgcttc gacgtcaccg acggcaagca tgagattgct gtgacctatg acggcgcttt
     1021 gcccgatctt ttccgtgagg ggcagggtgt tgtcgcggaa ggtgtcctgg cggccggcgg
     1081 cctcgatgct tcggaagttc tcgccaaaca tgatgagcgc tatatgccca aggaagtcgt
     1141 cgacgctttg aaaaagtccg gccgctggca ggaaaatgca gatatggggt cgagccaatg
     1201 attgtcgaaa ccggccattt cgcgctgatc ctggcgctgg cgctcgcctg cctcaatgcc
     1261 gtcgtgccgc tctggggcgt cgcgacacgc gattccaggc ttatgggtat cgcgccgacg
     1321 gtcgcgcttg ccggattcgg ttttgtcgct ttgtcttatg cagcgctgac ctatgcgcac
     1381 atcacgtccg atttctcgct cgtcaacgtc tatgaaaatt cggcctcagc gaagccgctg
     1441 atctataaga tcaccggcgt ctggggcaat cacgaaggct cgatgctgct ttgggtgctg
     1501 atcctcgccg tgttcgcggc ggcggtggcg ctctcggcgc gcgccatgcc gcaggatctc
     1561 catgccgccg tgctcgcggt gcaatcctgg atcagcgccg ccttcctgct tttcatcctt
     1621 cttacctctg acccgttcgc gcggatggcc aatccgccga tcgagggtca agacctcaat
     1681 ccgctattgc aggaccccgg tctcgccatc catccaccgt tgctttatct cggctatgtc
     1741 gggctttcga tcacctttgc tttcgccgcg ggcgcactga tctgcggccg gatcgatggc
     1801 gtctgggcgc ggcttgtccg gccgtggacg ctgatcgcct ggatattcct gacgctcggc
     1861 atagcgatgg gctcttactg ggcctattac acgctcggct ggggcggctt ctggttctgg
     1921 gacccggtcg agaacgcttc gctgatgccc tggctcgctg cgaccgcgct gcttcattca
     1981 gcaaccgtga tggagaaacg cgaggcgctg aaagtctgga cgatctttct cgccattctc
     2041 gcgttttcgc tctcgttgat tggtacgttc attgtgcgtt cgggcgtgct gacttcagtg
     2101 cattctttcg ccaacgatcc gcagcgcggt gagtttatcc tggcgattct cgtgttcttt
     2161 atcggcggcg cgctgctgct attcgccttg cgcgccaata ctttgaagcc cggcggcttc
     2221 ttcgcgccag tgtctcgcga gggggcgctc gttctcaaca atctcgtgct gacgacgtgc
     2281 tgcgccaccg tgttcgtggg gacgctttac ccattggcgc tccaatcgtt tacgggcgag
     2341 aaaatctctg tcggcggccc gttcttcaat gcgacggtcg tgccgctgtt cctgccgctc
     2401 gtgctactga tgcctgtcgg tcaaaatctc gcgtggaagc gcggcgattt tctcggcgcg
     2461 gcgcagcggc tctatttcgc actgctctgc ggccttgcga tggtgctggt gctcgcaggc
     2521 tggcatcata acggtccgct cacatcgatt atcgtcagcg ggcttggcgt ctacgtgatc
     2581 gtcggtgcct tcgccgaaat tgtgagccgt agcttcggcc ggatcgccct ggtcaagggc
     2641 ggcatcgctc aggtttggcg tcatgccacg ggcttgccgc tttcgaccta cggtacggcg
     2701 ctcgcgcatg cgggcatcgg cgtctctctg ctgggccttg cgggaacaag ctggggcgcc
     2761 gaaaaggtcc tcaccatgaa gccgggcgat agcgccattg tcgggcctta tcaattgacg
     2821 ctgacaagcc ttgtcagtga aaccggcccg aacttcaccg ccaaggtcgg ccatgtcgtc
     2881 attcgttcgg gcgaacgcga gatcgccgcg atcaatcccg ccgtgcgctt ctatccgacc
     2941 cggcagatga gccgtgccga agccggcatc aaaacgctca atctcgggca ggtttatgtt
     3001 ggcatcagcg acggcgtgaa gccgggcacg gtcgatgcgc gcctctattg gaaaccgctc
     3061 gtcacattga tctggatcgg cgccctcgtt atgggcttcg gaggcctcct ttcgctcagt
     3121 gatcgccggc ttcggattgg cgtcgcccac cgcttccgca agttcgccgg cgcgcagcag
     3181 gcgcccgccg aatgaagtgg ctcttgcgcc tcattgtgct tgcggtcctc acattggggc
     3241 cgctgctggc ccatgccgtt cagccggctg agatgctgtc cgatccgcag cttgaggcgc
     3301 gcgctcgggc gatttccgcc ggattgcgtt gtctcgtttg tcagaacgag tcgatcgacg
     3361 aatccaatgc gacattggcg catgatattc gcgtgctcgt tcgcgaacgt ctgctcgcgg
     3421 gtgacagcga cgagcaaatt cgcaattttc tcgtgtctcg ctacggcaat ttcattttgc
     3481 tgaagccgcc gttcgagccc gggacttatc ttctgtggct gacgccgctt attttgctgc
     3541 tcgccggcgg gggcgccatt tatctcaata cgcggcgaac gccgcccgca gcgcctgcaa
     3601 atctaacctc caccgaagcg accaagctcg cgcagatcgt cggccatacg gacgatctct
     3661 aagcctcctg gcgggtgagg attgcgggcg cccagcgatg ggcttcgcaa ccttacgaaa
     3721 gtttcattcc ctcgaaatag ccgcgtaagc cggccggcgt catcttcacg atcagagtcg
     3781 gacgccagcg ctcaagcccg cggccaatcc ggcagcaggt cgcggagccg tccgcatttc
     3841 gtttcgaacg ccgtcaatgg cacaggagaa gaataaaatg actcaaccgg tttctgaaaa
     3901 gaacccacaa tcgacggtga gaaaaagacc gcgcccgtcg cgcctcgtcc ttctcagcac
     3961 ggttgcggcg gtcgccttgg gcggcgcg
//
LOCUS       DASWOG010000583         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_271408,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000583 DASWOG010000000
VERSION     DASWOG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271408"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGB93_11895"
     CDS             complement(<1..511)
                     /locus_tag="VGB93_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0146822.1"
                     /translation="MADFFQTAFVTLLVTLDPPGLAPIFIALTLGMTKVEKREVAIRA
                     SIIAFAILFIFALGGATVMAALGISLPAFRIAGGLLLFYTAFQMIFAERGQRKKELAT
                     DAVTIDHVNNLAAFPLAMPLMSGPGSITAVILLAGRASGHWREQIGLILIILIVILMC
                     YIVFLISEQV"
     gene            complement(606..863)
                     /locus_tag="VGB93_11900"
     CDS             complement(606..863)
                     /locus_tag="VGB93_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0146823.1"
                     /translation="MSGDSLLIILVVGVVAGWLAGQILRGTGFGLVADLCIGIIGAFI
                     GNWILPKLGIHLGAHLLSQIISATIGAIVLLLILGLFRRRY"
     gene            1207..1722
                     /locus_tag="VGB93_11905"
     CDS             1207..1722
                     /locus_tag="VGB93_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0146824.1"
                     /translation="MAPRANWKGYLKLSLVSCPVALFPAVSRTERISFHLINRETGNR
                     LRQQYIDSVTGDIVERDEMVRGYEIGKNDYITLDESEIAEQAIESTHTIDIETFVPRN
                     EIDEVYLDGSYYLAPDDKVADEPFVVIRDAMRKSDMVGLARVVLAGRERIVMIEARDN
                     GLLATTLHYKY"
     gene            1780..2091
                     /locus_tag="VGB93_11910"
     CDS             1780..2091
                     /locus_tag="VGB93_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146825.1"
                     /translation="MLDLAAHIIDTKRGKFDPSKFDDRYQESLVDLIRAKRAGKPMPA
                     APQPKTPSNVINLMDALRRSVDAESGRRTRGKTSAKAHATAKKAVPAKSAAKRRVRKA
                     S"
     gene            2106..4736
                     /gene="ligD"
                     /locus_tag="VGB93_11915"
     CDS             2106..4736
                     /gene="ligD"
                     /locus_tag="VGB93_11915"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY0146826.1"
                     /translation="MALEAYHKKRNFDVTLEPRGAKGRGSGHSFVVQKHDATRLHYDF
                     RLELDGVLKSWAVTRGPSLVPGEKRLAVHVEDHPLDYGGFEGTIPAGQYGAGAVIVWD
                     RGTWTPDGDPHAGYAKGHLDFVLHGEKLKGRWHLVRMHPRPGEKGDNWLLIKGDDEAA
                     RGPRDPDILKEEPNSVITGRSIDDIAGDKKSRRWTSGRAAQGRVETPGSPRAHALAPL
                     ESVVKSDPAPVKRRSAKAAKTDEKATKPASQPAGKKTGGYAFTPPRGAKRKAQPDFLP
                     PMLPTLAAAPPSGTQWIHELKFDGYRIEAVAKAGRVRLRTRTGLDWTDRFPTIAVAIA
                     ALAGREFIIDGEVVVEEQNGVSDFSALQNALSEGDASGMVYYAFDLIAIDGYDLTSLS
                     VMERKEALAALLMTAPKDTILRLSEHFDENGDLMLRHVCRLGAEGIISKKRNSAYISK
                     RTAEWIKTKCANMQDFFIAGFVPSTSARNAIGSLVVGYYDKGELKHAGRVGTGYSNKM
                     AADLFADLSREKIAKPAFGAPLSAEARRNAVWVKPTRVAEVEFRGWTADANLRQASFK
                     GLREDKDPKEIVREKAAAMPNDAKTPKTSLKLTHPDRVLWPDAGVTKQGLADFYAMVW
                     PLMEKHLVGRPLVLLRCPNGIAQGGFFQKHPWAGIDDHILQIDDPHEEQPILGIKSFD
                     GLIALVQSAALEIHPWGARTDDLDRPDRLIFDLDPGEDVEFSAIIAGAHEVRQRLADK
                     KLKSFVKTTGGKGLHVVAPLKPAAAWTEVKDFCRGLAEAMAHDAPQSYTATVAKTQRT
                     GRIYVDYLRNARGATAVAAYSTRARPQAGVSTPLTWEELDSVLSGSQFTISNFNNRLQ
                     RLSDDPWAEFFRLRQSLPRK"
     gene            4797..4976
                     /locus_tag="VGB93_11920"
     CDS             4797..4976
                     /locus_tag="VGB93_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146827.1"
                     /translation="MTNHDTRYHSGTDEHVELKPNRARAGRKDVPILYVLLWSTALAV
                     LVLAAIFFGFHAMVH"
     gene            complement(5046..5426)
                     /locus_tag="VGB93_11925"
     CDS             complement(5046..5426)
                     /locus_tag="VGB93_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0146828.1"
                     /translation="MRVQAGLLSIIFAVSLAGASFADPLKYDTDKDGTLDLAEMEAAA
                     GAAFDRLNKDQDTTLEYKEAKGRLSKKAFVAADPDKDNSLTKDEYIAMAEKLFKAADL
                     ENDGTLDARELRSHAGRALQRLLK"
BASE COUNT         1168 a         1586 c         1686 g         1069 t
ORIGIN      
        1 ccacctgctc cgaaatcagg aacacgatgt agcacatcaa aatcactatg aggatgatga
       61 gaatgaggcc gatctgctcg cgccaatggc cactcgcccg gccggcgagc aggatcaccg
      121 cggtaatcga gccgggtccg ctcatcaggg gcatggcgag cggaaaagcg gcgagattgt
      181 tgacgtggtc gatcgtcacc gcgtcggtcg caagttcttt ctttcgctga ccacgctcgg
      241 cgaagatcat ttggaaagcc gtgtagaaga gcaaaagtcc gcccgcaatg cggaacgccg
      301 gcagggaaat gcccagcgcc gccatgacgg tggcgccgcc aagtgcgaag atgaaaagaa
      361 tagcgaaggc gatgatgctg gcgcgtatcg cgacttcgcg tttttcgacc tttgtcatgc
      421 ctagggttaa ggcgataaag atcggcgcga gccccggcgg gtctaaagtc acgagcagcg
      481 tgacgaaagc cgtttgaaag aagtctgcca tgggcttcgt ttccgtcgcg acgcgggggt
      541 tacacatgca aaagccgcgg cgcttgtatg cgcgccgcgg ctcaaataca tagcttgaac
      601 ttagctcaat agcgccgtcg gaacaagccc aggatcagga gcagcacgat cgcaccgatc
      661 gttgccgaga tgatttgcga cagcagatgc gcgccaaggt gaatgccaag cttcggtaat
      721 atccaattgc cgatgaacgc gccgatgatg ccgatgcaga gatcggcgac gaggccgaag
      781 ccggtgccgc gcaaaatctg gcctgcgagc cagccagcga cgacgccgac aacgagaatg
      841 atcaggagac tgtctccaga caaggcaaat ccctcttcat gtgagtgaac gataagccgt
      901 cctatgggca ggacgccgat agcggtgtgg cccgccgcac gaaggattaa cacacatcgc
      961 taaggagtcg tagcgtgtcc gttgctgcgg cgcgatgcaa gcgcaagatt aaaccctgcg
     1021 gggtggttac tcctctttac gccacaggcg tcgcggaaca ttttagtgtg atcggcgttg
     1081 aatttaccga atcttttggc gccttcattc gcgtggcgtt gcataagctt atgtggtgta
     1141 atgcgtgtag tttagcgtag ccaggagcgg cccgcccgct cccggtcagt actgagggtc
     1201 ttagccgtgg caccgcgtgc gaattggaag ggctatctca agctctcgct ggtttcctgc
     1261 cccgttgcgc tgttcccggc ggtcagccgg acggagcgca tttcgtttca ccttatcaat
     1321 cgtgaaaccg gaaaccggct ccgacagcaa tatatagatt ccgtgacagg cgatattgtc
     1381 gaacgcgacg aaatggttcg cggctacgaa atcggcaaga atgattacat cacgctcgat
     1441 gaaagtgaaa tcgctgagca ggcgatcgag agtacgcaca cgatcgacat cgagactttc
     1501 gtgccgcgca atgaaattga cgaagtctat ctcgacggct cttattacct cgcgcctgat
     1561 gacaaggttg cggacgaacc tttcgtcgtc atccgcgacg cgatgcgcaa gagcgatatg
     1621 gttggtcttg cgcgcgtcgt gctggccggc cgcgagcgta tcgtcatgat cgaggcgcgc
     1681 gacaatggcc tgctcgcgac gacgctgcac tacaaatatt aagtgcggga cgaaaagccg
     1741 tattttgaat cggttcccaa cgttaagttg tcgaaggaaa tgctcgatct cgccgcgcat
     1801 atcatcgaca ccaaacgcgg caagttcgat ccctcgaaat tcgatgatcg ctaccaggag
     1861 tcgctcgtcg atctgattcg cgcgaagcgt gccggcaagc cgatgccggc agcgccgcag
     1921 ccgaagacgc cgagcaatgt catcaatctg atggatgccc tgcggcgcag cgtcgatgct
     1981 gaatccggtc ggcgtacaag gggcaaaacg tccgcgaagg cgcatgccac ggccaagaag
     2041 gcggtgcctg cgaagtccgc ggcaaagcgg cgtgtgagaa aggcgagctg aaaggcggaa
     2101 cttgcgtggc gctcgaggcc tatcacaaaa agcgcaattt tgacgtcaca ttggagccgc
     2161 gtggcgccaa gggccgcggc agcggtcatt cgtttgtcgt gcagaaacac gatgcgacgc
     2221 ggttgcatta cgatttccgg cttgaactcg atggcgtgct gaagagctgg gcggtgacgc
     2281 gcggccctag cctggtgccg ggcgaaaagc gcctcgccgt tcatgtcgag gatcacccgc
     2341 tcgattacgg cggcttcgaa ggcacgatcc ccgcagggca atatggcgcg ggcgccgtga
     2401 tcgtctggga tcgcggaacc tggacgccgg atggcgaccc gcacgccggc tatgccaagg
     2461 gccatctcga tttcgttctg cacggcgaaa agctgaaggg ccgctggcat ctggtccgca
     2521 tgcacccgcg gccgggcgag aagggcgaca attggctcct gatcaaaggc gatgacgaag
     2581 cagcgcgggg accgcgcgat ccggatattc tcaaagaaga gcccaattcg gtcatcaccg
     2641 gacgctcgat cgacgatatc gccggggata agaaaagccg ccgctggaca tcgggccggg
     2701 cagcgcaggg ccgcgtcgag acgccgggtt cgccacgcgc gcatgcgttg gcgcccctcg
     2761 aaagcgtcgt caaatccgac ccggcgccgg tgaagcggcg ttcggcgaag gccgcgaaaa
     2821 ctgatgagaa ggcgactaag ccggcgagcc agccggctgg caaaaagacc ggcggctatg
     2881 ctttcacgcc gcccaggggc gcaaagcgca aggcgcagcc ggactttctc ccgcccatgc
     2941 ttccaacgct cgccgcagcg ccgccttcag gtacgcaatg gatccacgag ctgaaatttg
     3001 acggctaccg catcgaagcc gtggctaaag ctggcagggt caggctgcgg acacgcactg
     3061 gccttgattg gacggatcgt tttccaacga tcgcggttgc gatcgcagcg ctcgccggca
     3121 gggagttcat catcgatggc gaagttgtcg tcgaggaaca aaacggcgtc tcggacttct
     3181 cggcgttgca gaatgcgctg agcgaaggcg atgcaagcgg catggtctat tatgccttcg
     3241 acctgatcgc catcgacggc tacgatctca cgtccttatc cgttatggag cggaaggagg
     3301 ctctggcggc gctgctgatg accgcgccga aggacaccat tctgcgcttg agtgaacatt
     3361 tcgatgagaa tggcgatctc atgctgcggc acgtctgccg cctcggcgcc gaaggcatca
     3421 tctcgaagaa acgcaacagc gcctatatct ccaagcgaac ggcggaatgg atcaagacga
     3481 aatgcgccaa catgcaagat ttcttcatcg ccggtttcgt gccatcgacg tcggcgcgca
     3541 atgcgatcgg ttcgctggtg gtcggctatt acgacaaggg cgagcttaaa cacgccgggc
     3601 gcgtcggcac cggctattcc aacaagatgg ccgccgatct ctttgccgat ctgtcgcgtg
     3661 agaaaatcgc caagcccgcg ttcggcgcgc cgctctcggc agaggcgcgc cgtaatgccg
     3721 tctgggtcaa accaacacgg gttgcggagg tcgaatttcg cggctggacg gccgatgcaa
     3781 atttgcgcca ggcttcgttc aaaggtttgc gcgaagataa agacccgaaa gaaatcgttc
     3841 gcgagaaggc agcggccatg ccgaacgacg ccaagacgcc aaagacgagc ctcaagctca
     3901 cgcatcccga tcgcgtgtta tggccggacg caggtgtcac caaacagggg cttgcggatt
     3961 tttacgcgat ggtctggccg ttgatggaaa agcatctcgt cgggcggccg ctggttctat
     4021 tgcgctgtcc aaacggcatc gcgcagggcg gcttttttca gaagcatccc tgggccggta
     4081 tcgacgatca catcctgcaa atcgatgacc cgcatgaaga gcagccgatc ctcggtatca
     4141 agtcgttcga tgggctgatc gcgcttgtgc aatcggccgc gctcgaaatt catccatggg
     4201 gcgcgcggac cgacgatctc gatcgtccgg atcgtttgat cttcgatctc gaccccggcg
     4261 aagatgttga gttttccgcg atcattgccg gagcgcacga ggtacgccag cgccttgctg
     4321 acaagaagct caaaagcttc gtcaaaacga ccggcggcaa aggcctgcac gttgtcgccc
     4381 ccttgaaacc tgccgccgcc tggacggagg tcaaggattt ctgtcgcggt ctggctgagg
     4441 ctatggcgca cgatgccccg caaagctaca cagcgacggt cgccaagacg cagcggaccg
     4501 ggcgcatcta tgtcgattat ttgcgcaacg cgcgcggcgc aaccgccgtc gccgcctatt
     4561 cgacacgggc gcggccgcag gccggtgtat ccacgccgct gacatgggag gaactcgaca
     4621 gcgtcctcag cggcagccag tttacgatca gcaacttcaa caaccggctt cagcgactct
     4681 cggacgatcc gtgggcggag ttctttcgct tacgccaaag cctgccgcga aagtgagagg
     4741 ccataacgct ggaactcgtg gtagggcgca gagttgccgt gccaaaggag ccgcgcatga
     4801 caaaccatga cacgcgctat cattcgggca ccgatgaaca tgttgaattg aagcccaacc
     4861 gtgcccgcgc cgggcgcaag gacgtgccta tcctgtatgt cctgctgtgg agcacagctc
     4921 tcgctgtcct cgttctggca gcgattttct tcggcttcca cgcgatggtg cattaaatcc
     4981 atctgcaata ttcagttctt ccagcacggc caaggcaaag tcttggccgt gtttcctttt
     5041 gcgccctatt ttagaagacg ttgaagggcg cggccggcgt ggctccgcag ttctctggcg
     5101 tcgagtgtgc cgtcattttc gagatcggcg gctttgaaga gcttttcggc catcgcgata
     5161 tattcgtcct tggtcagcga attgtccttg tcaggatctg ccgccacgaa cgcctttttg
     5221 ctcaggcggc ctttggcttc tttatattcc aaagtcgtgt cctgatcctt attgagccga
     5281 tcgaatgccg cgcccgcggc cgcttccatt tcggcgagat cgagcgtccc atccttgtcc
     5341 gtgtcgtatt tgagcgggtc agcgaatgat gcacccgcga ggctgacggc gaagattatc
     5401 gatagcaatc cggcttgcac gcgcataggc gtctcccacg tttgccgctt tggggcaatc
     5461 acgccggaat tctagctgca gcaggctgtg cgtttgtagc ggacatatt
//
LOCUS       DASWOG010000584         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_234925,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000584 DASWOG010000000
VERSION     DASWOG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234925"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..462)
                     /locus_tag="VGB93_11930"
     CDS             complement(7..462)
                     /locus_tag="VGB93_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY0146829.1"
                     /translation="MPSFRTSRIVPHSAKAMFDLVADVESYPKFLPLCRALKVQRRET
                     LPDGRSLLVAEMEVGYKAIRERFTSRVTCDPEKLEILVEYIDGPFRQLENLWHFVPQG
                     ERSSKVEFDISYEFKSRTLAMLVGGMFETAFRKFAEAFESRADLVYRRS"
     gene            complement(520..1470)
                     /gene="lipA"
                     /locus_tag="VGB93_11935"
     CDS             complement(520..1470)
                     /gene="lipA"
                     /locus_tag="VGB93_11935"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0146830.1"
                     /translation="MTLVDAPKVPLRHPEKAHRPNQPVAPKPDWIRVKAPGSPAWRDT
                     AAIVKAHRLVTVCEEAGCPNIGECWEKKHATFMILGDTCTRGCAFCNVKTGLPGAVNP
                     HEPAHIAEAVAKLRLAHVVITSVDRDDLADGGAAHFAAVIGAIRAASPTTTIEVLTPD
                     FLRKPGALEIVVAAKPDVFNHNLETVPSKYSSVRPGARYFHSVRLLQRAKELDPQLFT
                     KSGIMVGLGETRDEILQLMDDLRSADVDFLTVGQYLQPTRKHHPVISFMPPPEFKALE
                     DIAWAKGFLMVSASPLTRSSYHAAEDFAKLGAARTNRQQI"
BASE COUNT          322 a          463 c          464 g          308 t
ORIGIN      
        1 gtcgttctaa gaacgccgat agacaagatc ggcgcgactt tcgaacgcct cggcgaattt
       61 ccggaacgcg gtctcaaaca tgccgccgac gagcatcgcg agtgtccggc ttttgaattc
      121 ataagaaata tcgaattcga ctttggacga acgctcgcct tgcggcacga aatgccagag
      181 gttttcgagc tggcgaaatg gcccatcgat atattcgaca agaatttcga gcttttccgg
      241 atcgcaagtg acgcggctcg taaaacgttc gcgaatggcc ttgtagccaa cttccatttc
      301 agccacgagc aaactgcgcc catcgggcaa cgtctcgcgg cgctgaactt taagcgcgcg
      361 gcagagcggc agaaatttcg gataggattc gacatccgca acaaggtcga acatcgcctt
      421 ggcggaatga ggaacgatcc ggctggtccg aaaactgggc atctttatcg tgataatttt
      481 agttaatgaa cgcggtcgca atgcaacgcc gcacgcggat caaatttgct gccgattcgt
      541 tcgtgcggcg cccagcttag cgaaatcttc cgccgcatgg taggacgagc gcgtcagcgg
      601 tgacgccgag accatcagaa agcccttggc ccacgcgata tcttcgagcg ccttgaattc
      661 cggcggcggc atgaacgaaa tcacgggatg atgtttgcgc gtcggctgga gatattggcc
      721 gacggtcaga aaatccacat cggcgcttcg caaatcgtcc atgagctgca aaatctcatc
      781 gcgcgtttca ccaagcccga ccatgatgcc ggacttggtg aaaagctgcg gatcaagctc
      841 tttcgcccgc tgtagcagcc gcaccgaatg gaaatagcgc gcgcccggac gcacgctcga
      901 atatttcgac ggcaccgttt cgagattgtg gttgaagaca tccggcttgg cggcgacgac
      961 aatctccagc gcgccgggtt tgcgcagaaa atccggcgtc agaacttcga tcgtcgtcgt
     1021 gggactcgcc gcgcggatcg cgccaatcac cgccgcaaaa tgcgccgcgc cgccgtcggc
     1081 aaggtcgtcc cggtcgaccg atgtgatgac gacatgggcg agccgcagct tggcaacggc
     1141 ctccgcgata tgcgccggct catgcggatt gacggcgccg ggcaggcccg tcttgacgtt
     1201 gcagaaagcg cagccccgcg tgcaggtgtc gcccaggatc atgaaggtcg cgtgcttctt
     1261 ctcccagcac tcaccaatgt tcgggcagcc agcttcctcg cagaccgtga cgagccggtg
     1321 cgctttcacg atcgcggctg tgtcgcgcca agcgggcgaa cccggcgcct tgacgcggat
     1381 ccagtccggt ttcggcgcaa cgggctggtt cggccgatgc gctttttccg ggtgccgcaa
     1441 gggcaccttg ggcgcgtcca ctagggtcat ctcaatctcc aggccagccc tattatatat
     1501 ggatggcgcg gccgtaggcg tcgagcacgc tctcgtgcat cgtctcggac agggttg
//
LOCUS       DASWOG010000585         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_326511,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000585 DASWOG010000000
VERSION     DASWOG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326511"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..406)
                     /locus_tag="VGB93_11940"
     CDS             complement(50..406)
                     /locus_tag="VGB93_11940"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0146831.1"
                     /translation="MKIAHVLVITFIGLSIPLPGKAQVAGTTLLGVSVAELRGVALGW
                     SAKHQILGQSVYNDKNERVGSIDDIIIAGDKAVSYAIVSAGGFLAVTKHDVAIPVSQL
                     KLVNDKFILPGAPKRR"
     gene            complement(426..860)
                     /locus_tag="VGB93_11945"
     CDS             complement(426..860)
                     /locus_tag="VGB93_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146832.1"
                     /translation="MDERLSEQLSDLSARVRSAKDAIEIAKTEAGEKLAARKEEARAA
                     AAAAIEKISTEIKSMRGTVDKNWNVIRAKIDADIDALIARVTADEHDLEVTHLANRAD
                     ESEWDAGFAIDYAAAAIEQAKFAVLDAIEARLKAEDAQHTRA"
     gene            complement(1162..>1764)
                     /locus_tag="VGB93_11950"
     CDS             complement(1162..>1764)
                     /locus_tag="VGB93_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0146833.1"
                     /translation="YRFIGELQIGIYTSLHCDIMGQTYAPREITLAYPSGSDFHLTEE
                     LAGCPLRFGQSANRIIFDSKWLDEPPRFGNRITYATTLAACDDLLADLSFRSGAAGKV
                     RDILLRNLAGRKTLSDAAQLLGTTSRTLSRQLRDQGTSFRTLSDQLRSQAAMKFLRET
                     TMTNEHIASALGFVDEANFRHAFQRWTGKTPSEYRRARRP"
BASE COUNT          338 a          490 c          528 g          408 t
ORIGIN      
        1 ccattaactg ccgtctgcta gtacgtgtat tcaaacactg ggctcgcctt cagcgcctct
       61 ttggtgcccc cggcaggatg aatttgtcgt tgacgagctt gagctgcgag acgggaattg
      121 cgacgtcatg cttggtaacg gcgaggaatc cgcccgcact gacgatggca taagagaccg
      181 ccttatcgcc ggcgatgatg atgtcgtcga tcgagccgac gcgctcgttc ttgtcgttgt
      241 agaccgattg gccgagaatc tggtgcttgg cgctccagcc gagcgcgaca ccccgcaatt
      301 cggcgacgct gacgccgagg agggtggttc ccgcaacctg cgcctttccc ggtaaaggta
      361 tgctcagacc gataaaagtt attaccaata catgcgcaat tttcacagaa acctcccttg
      421 tcccatcaag cccgcgtgtg ttgggcgtct tcggctttta gccgtgcctc aatcgcatcc
      481 agaacggcga atttggcttg ctcgatcgcc gccgcggcat agtcgatggc gaagccggca
      541 tcccactcag attcgtcggc acggttcgcc agatgagtca cctcgaggtc atgctcatct
      601 gccgtcacgc gcgcgatcag agcgtcgata tcggcatcaa tcttggcgcg aatgacattc
      661 cagtttttgt ccaccgtgcc gcgcatcgat ttgatctcgg tgcttatttt ctcgatggcg
      721 gcggccgcgg ccgcccgcgc ttcttctttg cgtgcggcga gcttttcgcc tgcttctgtt
      781 ttcgcaattt cgatcgcgtc cttggcgctc ctgacgcggg ccgaaaggtc gctcaattgt
      841 tcggaaaggc gctcgtccat gagtccactc tcaaatcggg tgacgatatt ttcgatcgga
      901 caatgatgcg cggatgcgcg tgctcgccag atgcacaggc agcatcgcgt tcggacggaa
      961 atttgtgaag gttgcgggag gacgtttatc ggtgcgattg ggacaaaatg gatttttgtc
     1021 cggctgtcgc tgatgtcgag ccgcagcatt cggcgtcttg gatgagaggg cggcgaaagg
     1081 aacctgcgtg gcgctactta tatcgccaac ataggttgca ttttacagca tggtcggtgc
     1141 ggaaattata cagattgcgg gctacggccg ccgggcgcgc cgatattcgc ttggcgtctt
     1201 tccggtccag cgttggaacg catgccggaa attcgcctcg tcgacaaagc ccagtgctga
     1261 cgcgatgtgc tcatttgtca tcgttgtttc gcgaagaaac ttcatcgcgg cctggctgcg
     1321 cagctggtcc gaaagcgtgc gaaaggatgt gccctgatcg cgcaattgcc ggctcaaggt
     1381 gcgggacgtc gtcccaagaa gctgcgcggc atccgaaagt gtcttccggc ccgcaagatt
     1441 gcggagcaaa atatctctca ccttgcccgc ggcgccgctg cggaatgaca ggtcggcgag
     1501 gaggtcgtcg caggcggcga gggtggtcgc gtaagtgata cgatttccaa accgcggtgg
     1561 ttcgtcgagc catttcgaat cgaagatgat ccgatttgcc gattggccaa agcgcaaagg
     1621 acaaccggcc aattcttcgg tcaggtggaa gtcgctgccc gacggatagg cgagcgtgat
     1681 ttcgcgtggc gcgtatgtct gccccatgat gtcgcaatgc aatgacgtat aaattcctat
     1741 ctgcaactcg ccgatgaaac ggta
//
LOCUS       DASWOG010000586         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_198448,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000586 DASWOG010000000
VERSION     DASWOG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198448"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VGB93_11955"
     CDS             <1..479
                     /locus_tag="VGB93_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146834.1"
                     /translation="LRDDTDVIIRAACGFFFVAAWANGGVILTPELKTNVALIPWIQL
                     ALAAGLIWRETLIFTALGIVGLFAYAVYEYGIFHLLDYPIFLGIAAYLGLTGLKRDLF
                     GMRPIDVVRYSAAITLMWASIEKWAYPEWTFPLFISHPAMSMGFDREFYMQSAGMV"
     gene            516..824
                     /locus_tag="VGB93_11960"
     CDS             516..824
                     /locus_tag="VGB93_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146835.1"
                     /translation="MRRVAAIILSAMFIAAVPAFGKIDAIGHAPIIAVMFGIIADDSV
                     SRRPIHLWLGIKENLARFKLAMSGWDLVLIPAAYCAALATFLAFYYVLHSVLYGTHIA
                     "
     gene            complement(892..1194)
                     /locus_tag="VGB93_11965"
     CDS             complement(892..1194)
                     /locus_tag="VGB93_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY0146836.1"
                     /translation="MILAASGAARTQQGPTPHVISQRGRAFNPNFLSIKQGDTVRIVN
                     DDGDLLHHAYVESDTFNFDSGDQLPGSKTDVVFSVRGQFNVLCCIHPKMKLVVVVK"
     gene            1553..>1956
                     /locus_tag="VGB93_11970"
     CDS             1553..>1956
                     /locus_tag="VGB93_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HEY0146837.1"
                     /translation="MSIITAALGGYAALGIRRAGVLVTKTFDESLMSINYARAASADF
                     ADMQVAFARRWSATKPETRAKLDQTMSDLEENLTEDLQIAAERSQSLRAARAAAEVQN
                     AADTWSEASRRLPENISFNADWGALDPYAETVS"
BASE COUNT          392 a          586 c          520 g          458 t
ORIGIN      
        1 gcctgcgtga cgataccgac gtgatcatcc gtgccgcttg cggatttttc tttgtcgcag
       61 cctgggccaa tggcggcgtc atcctgacgc ccgaacttaa gaccaatgtg gcgttgatcc
      121 cctggattca gctcgccctt gcagcagggc tgatctggcg cgagacattg atctttacgg
      181 cgcttggcat cgtcggactt ttcgcctatg ccgtgtatga atatggcatc tttcatctgc
      241 tggattatcc gatcttcctc ggcatcgccg cttatcttgg gctgacgggc cttaagcgag
      301 atcttttcgg catgcgaccc atcgacgttg ttcgctatag cgcagccatc acgctgatgt
      361 gggcctcgat cgagaaatgg gcttacccag aatggacgtt cccgctgttc atcagccatc
      421 ccgccatgag catgggtttt gaccgcgaat tctatatgca atcggcgggc atggtctaat
      481 tcaccctgtc ctttgcactc atcctcacgc cgctcgtgcg ccgcgtcgcg gccatcatcc
      541 ttagcgcgat gttcattgcg gcggtgccgg ctttcggcaa gatcgatgcg atcgggcacg
      601 cgccaatcat cgcagtcatg ttcggcatca ttgccgatga ttcggtgagc cgccgcccga
      661 tccacctgtg gctcggcatc aaggaaaatc ttgccaggtt caaattggcg atgtcgggct
      721 gggatctcgt tctgatcccg gccgcctatt gtgccgccct cgcaacgttc ctggcgttct
      781 attatgtgct gcactccgtg ctctacggaa cgcacatcgc ctgaggcttg cgcgcataaa
      841 tcgttctgca agaagctccg agaaactcgg agcttctttt ttgcggcatc gtcacttgac
      901 gacgacgacg agcttcattt tcggatgaat gcagcagagg acgttgaatt ggccacgcac
      961 tgaaaagacg acgtccgtct tgctgcccgg caattgatcg cccgagtcga agttgaatgt
     1021 atccgactcg acataggcat gatgcagcag atcaccgtcg tcgttcacga ttcgcacggt
     1081 atcgccctgc tttatgctca gaaaatttgg gttgaaggcg cgtccacgct gcgaaatcac
     1141 gtgcggcgtc gggccctgct gcgtgcgcgc agcaccgcta gcagccaaga tcaaggctgc
     1201 tgccagtact gccatcggaa gccggacccg cttacctgac attttaagcc cagaggtcgg
     1261 atactcgctt ttcgtcgcct cgtcagcgac gcaactttcc cgagaaccga ttcggtcatt
     1321 agttaactaa aagcaaaatc accacttttg ggcgtggtta gacaagcgaa tttagcctcg
     1381 tcttaactcc caaggccgca aaagaaacta ttcaattcga gttattttga tgaatgtccg
     1441 tcttgcatcg ccgccagcat atggtacggc gacaccgctg cgctcgccgt taatgcgttt
     1501 tgctcaattg acgcaaacga tccgcggccg cattttaaca gcctttttgg ccatgagcat
     1561 catcaccgcc gcgctcggtg gttatgccgc gcttgggatc cgccgcgctg gcgtattagt
     1621 caccaaaacc ttcgacgaat ccttaatgtc gattaattat gcgcgtgcgg cgtctgctga
     1681 tttcgccgat atgcaggtcg ctttcgcgcg ccgctggagc gcgacgaagc ccgaaacccg
     1741 ggccaaactc gaccagacaa tgtcggacct tgaagaaaac ctgacagaag acctgcagat
     1801 cgcggcagaa cgctcacaat ccttacgcgc ggcacgcgcc gctgcggaag tccagaacgc
     1861 cgccgatacc tggagcgaag cgagccgccg cctgcccgaa aacatctcct tcaacgccga
     1921 ctggggtgca cttgaccctt acgccgagac ggtgtc
//
LOCUS       DASWOG010000587         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_69010,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000587 DASWOG010000000
VERSION     DASWOG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69010"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VGB93_11975"
     CDS             <1..1186
                     /locus_tag="VGB93_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CtpF protein"
                     /protein_id="HEY0146838.1"
                     /translation="QAFCETTEMAQVIQAAIDDRRMEKAHVKLHLGGPAAAIEAYRSA
                     PTPNVIFLEATAKREELVQYLTALAEFCDAGTKVVVTGRMNDIVLYRDLMARGVSEYL
                     VAPFTVLDFIRAISKLYTSHGADPVGKVVAFVGAKGGVGASTLAHNVAWSIPRDFEIQ
                     TALVDLDLGFGTAGLDFNLDPPQGIAEAVFAPDRVDSNLIDRLLSRCGDKLGLLAAPA
                     TLDRMYDFPETAFDSIVDILRASTPCVILDIPHGWTAWMRRMIVGADEVVLVAAPDLA
                     NLRNTKSLLDTIRAARPHDAKPKLVLNFVGVPKRPEIAAADFAKAVDLEPTGIINFEP
                     KLFGTGANNGQMIAEVEPTSKASETILDLARAVTGRPDMRKAKKNLFEPFKLKLGSKR
                     AS"
     gene            1333..>2627
                     /locus_tag="VGB93_11980"
     CDS             1333..>2627
                     /locus_tag="VGB93_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0146839.1"
                     /translation="MFGKRSFASENAPVRAATPAAAITNFRGVTDTVRKAAPVTIDPP
                     AAPPPAAQEAPRSDEYYVTKSMIFGALIEGIDLSQLARLDADSAREEIRDIVNEIIAI
                     KNIVMSISEQEDLLTDICNDVLGYGPLEPLLARDDIADIMVNGATRTFIEVGGKIQLT
                     SVRFRDNSQLMNICQRIVSQVGRRVDEASPICDARLADGSRVNVIAPPLAIDGPVLTI
                     RKFRKDKLTLDQLIRYGCISPEGGEILKIIGRVRCNVLISGGTGSGKTTLLNCLTNYI
                     DDGERVITCEDAAELQLQQAHVVRLETRPPSLEGTGAVTMRDLVRNCLRMRPERIIVG
                     EVRGPEAFDLLQAMNTGHDGSMGTVHANSPREALSRIESMITMGGFALPQRTIREMLV
                     SSIDVIIQTARLRDGSRRITHVTEIMGLEGDVVTMQDLIL"
BASE COUNT          532 a          820 c          768 g          507 t
ORIGIN      
        1 gcaggcgttt tgcgagacga ccgagatggc gcaagtcatc caagcggcga tcgacgaccg
       61 gcgcatggag aaagcgcacg tcaagctgca tctgggcggc cctgccgcgg cgatcgaggc
      121 ctatcgctcg gcccccacgc cgaacgtgat cttcctcgag gcgacggcca aacgcgaaga
      181 gctggtccaa tacctgacag cacttgccga attctgtgac gccggcacca aggtggtggt
      241 gacggggcgg atgaacgata tcgtgcttta tcgcgacctg atggcgcgcg gtgtcagtga
      301 atatctcgtc gcaccattca cggtgctcga tttcatccgc gcaatttcaa agctgtatac
      361 gagccacggc gccgatcctg tcggcaaagt ggtggccttt gtcggcgcca aaggcggcgt
      421 cggcgcctcg acactcgcgc acaacgttgc ctggtcgatt ccgcgcgatt ttgaaatcca
      481 gacagccctt gtcgatctcg atcttggctt cggcaccgcc gggcttgact tcaatttgga
      541 ccccccgcaa ggcatcgccg aggcggtctt tgcgccggac cgcgtcgatt ccaacctcat
      601 cgaccggcta ctgtcccgct gcggcgacaa gcttggcctc ctggctgcgc ctgccacact
      661 cgaccgcatg tacgattttc ccgaaaccgc tttcgattcg atcgttgaca tcctccgggc
      721 ttcgacgccc tgcgtcatcc tcgacattcc gcacggctgg acggcttgga tgcggcggat
      781 gatcgtgggt gccgacgaag ttgtgctcgt cgccgcgcca gacctcgcca atttgcgcaa
      841 caccaaaagt ctgctcgata cgatccgggc cgcccggccg cacgatgcca aaccaaaact
      901 cgtcctgaat tttgtcggcg tgccaaagcg tccggaaatc gcagcggcgg atttcgccaa
      961 ggcggtcgat cttgagccta ccggcatcat caatttcgaa ccaaagctct tcggtaccgg
     1021 ggccaacaac ggacagatga tcgcggaagt ggagcccacg agcaaagcca gcgagacaat
     1081 cctggatttg gcccgcgcgg tcaccggccg gccggatatg cgcaaggcca agaagaacct
     1141 atttgagcca ttcaagttga agttgggctc aaagcgggcc tcgtaatacc cggtccccgg
     1201 gcgggatttt gcgcatcgcg ctaaatccga agggtatttt gtaggaattt ttttagggct
     1261 taggcgatct tttaagccga cgctggcaga atgcgtgcgc gccgcgtggc gtccacgaaa
     1321 gaggagcaag gcatgtttgg caagcggtcg ttcgccagcg aaaacgcacc cgtgcgtgcc
     1381 gcaacgcccg cggccgcgat taccaacttt cgcggcgtca ccgacacggt gcgcaaggca
     1441 gcacccgtca ccatagaccc gccggctgcc ccgccgcccg cggcccagga agcgccgcgc
     1501 tccgacgaat attacgttac gaaaagtatg attttcggcg ccctgattga gggcatcgac
     1561 ctatcacaat tggcgcgtct cgatgccgac agcgcacgcg aagaaatccg cgacattgtc
     1621 aacgagatca tcgcgatcaa aaacatcgtg atgtcaattt ccgagcaaga agacttgctc
     1681 accgacatct gcaatgacgt tctgggctac ggcccgcttg agccgttact cgcccgcgac
     1741 gacatcgccg acatcatggt caacggcgcg acgcggactt ttatcgaagt cggcggcaag
     1801 atccagctga caagcgtgcg ttttcgcgac aattctcagc tcatgaacat ttgtcagcgg
     1861 attgtgagtc aggtcggccg ccgtgttgac gaagcctcac cgatctgcga cgcgcgcctc
     1921 gccgacggct cccgtgtcaa cgtcatcgcg ccaccgctcg ctatcgacgg tccggtcctg
     1981 acgatccgta aatttcgcaa agacaaactt acgctcgatc agttgatccg ctatggctgt
     2041 atatcgcccg agggcggtga gatcctgaag atcatcggcc gcgtccgctg caacgttctg
     2101 atctccggcg gcaccggctc tggcaaaaca acgctgctta attgcttgac caattatatc
     2161 gacgatggcg aacgcgttat tacctgcgaa gacgccgccg aattgcaatt gcagcaggca
     2221 cacgtcgtcc ggcttgagac gcggccgccg agtctcgaag gcacgggcgc ggtgacgatg
     2281 cgcgacctgg tcagaaactg cctgcgtatg cgtcccgagc gtattatcgt cggcgaggta
     2341 cgcggacccg aggctttcga cctgttgcag gcgatgaaca cgggccatga tggctcgatg
     2401 ggcaccgtcc acgcaaactc gccgcgggaa gccctgagcc gtatcgaatc catgattacg
     2461 atgggcggtt tcgccctgcc gcagcgcacg atccgggaga tgctggtctc ctcgatcgac
     2521 gtgatcattc agaccgcgcg cctgcgcgac ggttcgcgcc gcattacgca tgtgaccgag
     2581 atcatgggcc tcgaaggcga cgtcgtgacg atgcaggacc tgatcct
//
LOCUS       DASWOG010000588         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_32939,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000588 DASWOG010000000
VERSION     DASWOG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32939"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1824
                     /locus_tag="VGB93_11985"
     CDS             208..1824
                     /locus_tag="VGB93_11985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY0146840.1"
                     /translation="MTFHYAPIFPATKDETPYRKLTDKHVRIEKFGPRDMLVVEPEAL
                     RLLAEAAMIDINHLLRPGHLAQLAKILDDPEATPNDKFVAYDLLKNANIAAGGILPMC
                     QDTGTAIVMGKKGRLVFTEGGDEAALAEGIWNAYEKKNLRYSQLAPLSMFEETNTKTN
                     LPAQIEIYSDGEDAYKFLFMAKGGGSANKTFLYQGTPSLLTHDRMIAFLKEKILTLGT
                     SACPPYHLAIVIGGTSAEINLKTVKLASTRYYDTLPSVGSTDARAFRDRELEEEIFAL
                     TQNLGIGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQALGKITKDGVFLEE
                     LEHDPAKYLPPIAKEDLSGNVVHIDLARPMPEILATLSQYPIRTRLSLSGPMIVARDL
                     AHAKLREKLERGEPLPDYFKNHPVYYAGPAKTPTGYASGSFGPTTAGRMDSYVDQFQA
                     AGGSMVMLAKGNRSSQVREACAKHHGFYLGSIGGAAAQLAQDCITKVEVLAHPELGME
                     AIWRIEVKNFPAFIVIDDKGNDFFKELNLG"
BASE COUNT          408 a          551 c          520 g          391 t
ORIGIN      
        1 aaatcgttgc gcgcactgcg gcaggacggc ttcacgcccg ccgagatcaa ggcccacctg
       61 ggcttgaatg agcgcagctt tgctttcgtg ccttagaagg cttagtaaat tcggcagaat
      121 cgacgccacg tccgggggtt gctatagcaa ccccaaagcg agtgcgcccg ccgtctcccg
      181 gcgcggccag aggacgaaag cgccgcgatg acgtttcact atgccccgat ctttcctgct
      241 accaaagacg agacgcctta ccgcaaattg accgacaagc atgtgcgcat cgaaaaattc
      301 ggtccgcgcg atatgctcgt tgtcgagccg gaggcgctgc ggcttctcgc tgaggcggcg
      361 atgatcgaca tcaatcatct gctgcggccg ggccatctcg cacagctcgc aaaaattctt
      421 gacgatcccg aagcgacacc caacgataag ttcgttgcct atgatttgct caagaacgcc
      481 aatattgccg ccggcggcat tttgccgatg tgtcaggaca ctggcaccgc catcgtgatg
      541 ggcaagaagg ggcgtctcgt cttcaccgaa ggcggcgacg aggcggcgct cgccgaaggc
      601 atctggaacg cttacgaaaa aaagaacctg cgctattcgc aacttgcgcc gctctcgatg
      661 ttcgaagaaa cgaataccaa gaccaatcta ccggcgcaga tcgaaatcta tagcgacggc
      721 gaggacgcct ataagttctt gttcatggca aagggcggcg gctccgccaa caagaccttt
      781 ctatatcaag gcacgccatc gttgctgacg cacgaccgga tgatcgcctt tctgaaagag
      841 aaaatcctca cgctcggcac gtctgcctgc ccgccctatc atctcgccat cgtcatcggc
      901 ggcacgtcgg ccgagataaa cctgaagaca gtcaagctcg catcgacgcg ctattacgat
      961 acgctgccgt ctgttggcag cacggacgcg cgggcgtttc gcgaccgcga gctcgaggaa
     1021 gaaattttcg cgctgaccca aaatctcggc atcggggcgc aattcggcgg taaatatttc
     1081 tgtcacgatg tccgcgttat tcgtctgccg cggcatggag cttcgctgcc cattgggctt
     1141 ggtgtttcat gttccgccga tcgtcaggcg ctcggcaaga tcaccaagga cggcgtgttt
     1201 ctcgaagaac tcgaacatga tccggcgaaa tatctgccgc ccatcgcgaa ggaagatttg
     1261 agcggcaatg tcgtccatat cgacctcgcc cggccgatgc cggaaattct cgcgacgctg
     1321 tcgcaatatc caatccgcac ccgactttct ctaagcgggc cgatgatcgt cgcgcgcgat
     1381 ctcgcccacg cgaagctgcg cgagaagctc gagcgcggtg agccgctgcc ggattatttc
     1441 aaaaatcacc cggtctatta cgccggcccg gcgaagacgc cgacaggata tgcatccggt
     1501 tccttcggac ccacgaccgc gggccggatg gattcctatg tcgatcaatt tcaggccgcc
     1561 ggcggctcga tggtgatgct agcaaagggc aatcgctcgt cgcaagtacg agaggcgtgt
     1621 gcgaaacatc acggcttcta ccttggttcg atcggcggag cggctgcgca acttgcgcag
     1681 gattgcatca ccaaggtaga ggttcttgca catcccgaac ttgggatgga agcgatctgg
     1741 cgcattgagg tcaagaattt ccctgcgttc atcgtcatcg atgacaaggg aaatgatttc
     1801 ttcaaggaac taaacttggg ctaaagcgtt cgttcattgt cctattcttt tacgcgtctt
     1861 ttcgtttgtc
//
LOCUS       DASWOG010000589         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_87157,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000589 DASWOG010000000
VERSION     DASWOG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87157"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGB93_11990"
     CDS             <1..246
                     /locus_tag="VGB93_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0146841.1"
                     /translation="HYDGIVAFSQTDFTEDLKKINVPVLVMHSEDDQIVPYADAGPLS
                     AKLLKNGTLKTYKNYPHGMPTTEAPTINADLLAFLKG"
     gene            complement(283..2142)
                     /gene="phaC"
                     /locus_tag="VGB93_11995"
     CDS             complement(283..2142)
                     /gene="phaC"
                     /locus_tag="VGB93_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY0146842.1"
                     /translation="MNEAVLPPEATKPVAAPGPKILPQIEALAADAPKIDFARFSKNL
                     ATLIEQGGHALSVMLQPQADRSANGFAESVVDAVQAFGRIAEYWLSDVERTNAARAAL
                     AEHFLSLWAQTFRRMSGEIALPVVPVAPGDKRFAAPQWQESPLFDFLRQAHAIATQWV
                     EDLVARTETDPMTKLKAQFYLRQLASALSPSNFVATNPELLHETLASSGDNLVRGIAN
                     LAADIKAGNGSLRIRQSDSSKFMLGVNMAATPGKVIIRNDLMELIQYTPTTEIVFKRP
                     LLIVPPWINKYYILDLNPDKSFVRYAVEQGHTVFLISWVNPDARHRDKDFEAYIDESI
                     MTVLDVIAEVTGESEVNALGYCVGGTLLSIALAYMARKNDHRIASATFFTTQTDFADA
                     GDLRVFIDEAQISALEESMAEKGYLDGAKMANVFNMLRPNDLIWSFIVNNYIRGQSPM
                     AFDLLTWNSDATRMTPACHSFYLRNCYLENKLSRGRLNIRGTPVHLNDIKIPTYSLAT
                     REDHIAPASSVFTGMGMFGGDVRFVVAGSGHIAGVINPVTKPKYQYWTGGALRGTYED
                     WLKAAKEHPGSWWPDWAEWIAAQGPKKVPAREPGGDALMPLCDAPGTYVRAKS"
     gene            2405..2842
                     /locus_tag="VGB93_12000"
     CDS             2405..2842
                     /locus_tag="VGB93_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146843.1"
                     /translation="MTLASAQAQQADSSQPAPKKTPQPYGGGTPLDVILHNKLWVTPP
                     PMAPFVKASRPPEATLEYTPTSGKEVTRPALRNAAQLQQLQDELEDGGAHNETLTHSK
                     VRHFQALPKKVVLKKVATKKLKKKTLASSPMRITPTSVADPRS"
     gene            complement(2808..3587)
                     /locus_tag="VGB93_12005"
     CDS             complement(2808..3587)
                     /locus_tag="VGB93_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146844.1"
                     /translation="MAEQSPRAQPFSGRVALVTGASRGIGHALALELARAGAHVIALA
                     RTQGALEELDDAIRAAGSEATLVPCDLTDFDALDRLGAAIHERWKKLDIFVGNAGELG
                     AITPLSHIDPQDWDKVIAVNVTANWRLIRALDMPLRAAEAGRVLFITSGVAHRPDYRP
                     YWGGYAVSKAALDALARTYAAETETTSDVKVMLANPGPLRTRMRAKAMPGEDPLTLKT
                     PEDLAPKLLQFLLPSSSQTGQLYDFPSDKILSFAGPQPTSV"
BASE COUNT          703 a         1055 c         1125 g          717 t
ORIGIN      
        1 cattacgacg gcatcgtcgc cttctcgcag acggacttca ccgaggatct caagaagatc
       61 aatgtgcctg tgctcgtgat gcacagcgaa gacgatcaga tcgtgcccta cgccgacgcc
      121 gggccgctat ccgcgaagct gctgaagaac ggcactttga aaacctacaa gaattatccg
      181 cacggtatgc cgacgacgga agcgccgacg atcaacgccg atcttctggc tttcttgaag
      241 ggctagcgaa cagcgccgtg ccgggacatc cggcgcggcg tctcagctct tcgcgcgcac
      301 atacgtgcca ggtgcgtcgc agagcggcat aagcgcatcg ccgccgggtt cgcgcgcggg
      361 cacctttttc gggccttgcg cggcgatcca ttcggcccaa tccggccacc agcttccggg
      421 atgttccttg gccgctttca gccaatcctc gtaggtcccc ctcagcgcgc cacccgtcca
      481 atactgatat ttgggcttgg tgactgggtt gatgacgccg gcgatatggc cggagccggc
      541 aacgacgaag cgcacgtcgc caccgaacat gcccatgccg gtgaaaaccg agcttgctgg
      601 cgcgatatga tcttcgcgcg tcgcaagact gtacgtcgga attttgatgt cgttgagatg
      661 tacgggcgtg ccgcggatat tcagccgtcc gcggctcaat ttattttcga ggtagcaatt
      721 gcgcagatag aacgaatggc aggctggcgt catccgcgtg gcatcggaat tccacgtcag
      781 gagatcgaat gccattggcg attggccgcg gatgtaattg ttgacgatga aagaccagat
      841 cagatcgttc ggccgcagca tgttgaagac attcgccatc ttggcgccgt cgagataacc
      901 tttctcggcc atgctttcct caagcgcgct gatctgggct tcatcgatga agacgcggag
      961 atcgccggcg tcggcgaaat ccgtctgtgt cgtgaagaat gtcgcgctgg cgatgcggtg
     1021 gtcgttcttg cgcgccatat aggcgagtgc gatcgagagc agcgtgccgc cgacacaata
     1081 gccgagcgca ttgacttcgg attcaccagt tacttcggcg atgacgtcga gtaccgtcat
     1141 gatgctctcg tcgatatagg cctcgaaatc cttgtcgcga tggcgcgcgt cgggattgac
     1201 ccaggagatc agaaagaccg tgtggccttg ttcgacggca tagcgaacga agcttttgtc
     1261 cgggttgaga tcgaggatgt agtatttgtt gatccacggc gggacgatta gcagcggccg
     1321 tttgaacact atctcggtcg tcggcgtgta ctggatcaat tccatcagat cgttgcggat
     1381 gattaccttg cccggagtcg ccgccatatt gacgccgagc atgaatttcg aggaatcgct
     1441 ttgcctgatg cggagcgaac cattacccgc cttgatgtcg gcggcgagat tggcgatgcc
     1501 gcgcacgaga ttgtcgccgc tcgaagcgag cgtttcgtgc agcagctccg gattggtcgc
     1561 gacgaaattc gacggcgaca aagcgcttgc caattgccgc agatagaatt gcgccttcag
     1621 cttcgtcatc gggtcggtct cagtgcgcgc gacgagatcc tcgacccatt gcgttgcgat
     1681 ggcgtgtgcc tgacgcagga aatcgaacag cgggctttcc tgccattgcg gcgcggcaaa
     1741 gcgtttatcg cccggcgcga ccggaacgac aggcagcgca atttcgccgg acatgcggcg
     1801 gaacgtctgc gcccacagcg acagaaaatg ctcggcgagg gcggcgcgcg ctgcgttggt
     1861 gcgttccacg tccgaaagcc aatattcggc aatgcggccg aaggcttgaa ctgcatcgac
     1921 aacgctttcg gcgaaaccat tcgccgaccg atcggcctgc ggctgcaaca tgaccgagag
     1981 cgcgtggccg ccctgttcga taagcgtcgc taggtttttc gagaagcgcg cgaaatcgat
     2041 cttcggggca tccgctgcga gcgcttcgat ctgcggcaga attttcgggc cgggcgcggc
     2101 cacaggcttg gtcgcttccg gcggtaacac ggcctcgttc acggacgctt tccttccggc
     2161 agccgcccgg gcccgactcg tcgccggggc gcgcttttgg ctcgggctgc gcgcctttcg
     2221 ccgtttggcc gctgctgttt ccttaaccac tcacccaccg tgccatgata gggctttatt
     2281 cccttcttag ggaatagggg tcgcggggct gcgggacaac cgcgcattga gccggacatg
     2341 tagagagatg tggacttccc ggacggcatt tcaaataggt ttgggaatag ctctatcggg
     2401 gctgttgacc cttgcttcag cgcaagcgca gcaagccgat tcgtctcagc ctgcgccgaa
     2461 gaaaacgcca cagccttatg gcggcggcac gccgctcgac gtcattctgc ataacaaatt
     2521 atgggtgaca cccccgccga tggccccctt cgtcaaggcg tcgcggccgc cagaggcgac
     2581 gcttgaatat acgccgacct ccggaaaaga ggtgacgcgt ccagcattgc gcaacgcggc
     2641 acagcttcag cagttacaag acgaactaga agacggcggc gcgcataacg aaaccctcac
     2701 gcattcgaag gtccggcatt ttcaggctct gccgaaaaag gtcgtgttga agaaggtcgc
     2761 gacaaagaag ctgaagaaaa agacgctcgc atcgtcgcca atgcgtatta caccgacgtc
     2821 ggttgcggac ccgcgaagct gagaatttta tcgctcggga agtcgtaaag ctggccggtt
     2881 tgcgatgacg acggcagcag gaattgcaga agcttcggtg ccaaatcttc gggcgtcttg
     2941 agcgtcaatg ggtcttcgcc cggcatggcc ttggcgcgca tccgcgtgcg tagcggcccc
     3001 ggattggcca gcatgacttt tacgtcggag gtcgtctcgg tctctgcagc ataggtccgc
     3061 gccaaagcat cgagcgcagc tttggaaacg gcgtagccgc cccaataggg ccggtagtcg
     3121 gggcggtgcg cgacacccga cgtgatgaaa agaacccgtc ctgcctcggc cgcccgcagc
     3181 ggcatatcga gcgcgcggat cagccgccag ttcgcggtga cgttgacggc gatgactttg
     3241 tcccaatctt gcggatcgat atgcgaaagc ggcgtgatgg cgccgagttc gcctgcgttg
     3301 ccgacgaaga tgtcgagctt tttccatcgc tcgtggatag ccgcgccgag acggtcgagc
     3361 gcgtcgaaat ccgtcaggtc gcacgggacg agtgtcgcct cgctgcccgc ggcgcgaatc
     3421 gcatcgtcaa gctcttccag agcgccctgg gtgcgggcga gggcgatgac atgcgcaccg
     3481 gcgcgggcaa gttcgagtgc aagcgcatgg ccaatgccgc gcgaggcacc ggtgacgagt
     3541 gccacgcgac cggagaatgg ctgtgcccgg ggactctgct cagccatgtt agatctcgag
//
LOCUS       DASWOG010000590         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_252815,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000590 DASWOG010000000
VERSION     DASWOG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252815"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1206
                     /locus_tag="VGB93_12010"
     CDS             37..1206
                     /locus_tag="VGB93_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0146845.1"
                     /translation="MSNNKSVGGRRGPHNISGALSALVSFCSIVALGFAFTIARGAAD
                     PVKPPYIQFIGHTADLTDAEYSADGKRVLTASSDKTARIWNAANGVSLITLSGHTAAV
                     RTARFNADGSRVLTAAQDSTAKIWDAANGKLLFTLTGHTDPLEDAEFSPDGKFVVTAS
                     RDATAKIWDAGTGALVATLTGHSGPVMHAVFSPDGRRVATASEDHSARIWDAHTGALV
                     FTLTGHEGIVTGVSYSPDGRLIATSSSDKTARIWNAQTGVLVTILQGHKRAVEFAIFN
                     HDGTKILTGSTDKLGILWDAKSGAKLATLLGHRRAIMSASFSPDDKRIATASYDSTAR
                     IWDAASGTSQQVLAGHGGEVTRAKFSPDGQHVLTASFDTTARAWSVGGSSVGAPK"
     gene            1272..2102
                     /gene="folP"
                     /locus_tag="VGB93_12015"
     CDS             1272..2102
                     /gene="folP"
                     /locus_tag="VGB93_12015"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0146846.1"
                     /translation="MQQKRDQFLALIAARSAIMGILNVTPDSFSDGGKFQSHDAAIAH
                     ARKLTGEGADIIDVGAESTRPGHVPVPVEEELARLKPVLADIVNATPLPVSIDTSKAP
                     VARLAIEMGAAVVNDVWGLQRDPAMADVVAAGGAAAVVMHNRENIDPALDILSDMRRF
                     FDHSLELAEKAGIPRRYLILDPGIGFGKTKDQNLIALRRLDHFKDYGLPLLVGVSRKS
                     LFAPILGAAVDERMIGTVATNIIAAARGASIFRVHDVAEHVAAFKVFDAIERNPAAAL
                     "
     gene            2099..2593
                     /gene="folK"
                     /locus_tag="VGB93_12020"
     CDS             2099..2593
                     /gene="folK"
                     /locus_tag="VGB93_12020"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0146847.1"
                     /translation="MSETGLVEIGLGLGSNIGDAPGNVREALRLLRASDAIEITKVSS
                     IYRTAPWGYKDQAAFANACALGRSKLNPERLLASVKAIEAEMGRTKTVRWGPRLIDID
                     ILFYGTQSLVSDSLVLPHKELFNRAFVLVPLTEIAPDLVLGGRPVAEAAAAIRHDDIE
                     KWDP"
BASE COUNT          497 a          792 c          848 g          502 t
ORIGIN      
        1 ggtggcaaag aggtcccagc aaaaaggacc aagcgaatgt ccaataataa atctgtcggc
       61 ggccgccggg ggccgcacaa catctccggt gctttgagtg ctctggtgtc tttctgtagt
      121 attgttgcgt tagggtttgc gttcacgatc gcgcgcggcg cggccgatcc ggtcaagccg
      181 ccttatatcc agttcatcgg ccatacggcc gatctcaccg acgcggaata tagcgccgac
      241 ggcaagcgcg tgcttacggc ttcgagcgac aagacggcgc gaatctggaa tgccgccaac
      301 ggcgtctcgc tgattacgtt gagcggtcat acggcggccg tacgcacggc gcgtttcaac
      361 gctgatgggt cgcgcgtgtt gaccgcagca caagattcca ccgcgaaaat ctgggacgcc
      421 gcgaatggca agcttctgtt cacattgacg ggccacaccg acccgcttga ggatgcagaa
      481 ttcagccccg acggcaaatt cgtcgtcact gcgtcgcgcg acgccacggc gaagatttgg
      541 gacgccggaa caggcgcatt ggtcgccacg ctcacagggc atagcggccc ggtgatgcat
      601 gccgttttca gtccggatgg gcggcgggtc gcgacggcct cggaggatca tagcgcccgt
      661 atctgggatg cccacacggg cgcgctggtt ttcacgctga cgggccatga aggcatcgtc
      721 acgggggtga gctacagccc ggacggtcgg ctgatcgcca cctcatcgag cgataagacg
      781 gcacgcatct ggaatgcgca gaccggcgtt ctcgtgacga ttctgcaggg tcataaacgt
      841 gccgtcgaat tcgcgatctt caatcacgat gggacgaaga tattgaccgg ctcgactgac
      901 aagttgggca ttctttggga cgccaaatcg ggtgccaagc ttgccacctt gctcggccac
      961 cgccgcgcca tcatgtcggc gagcttcagc ccggatgaca agcgcatcgc aacggcatct
     1021 tacgattcga cggcgagaat ctgggacgcg gcaagcggca catcgcagca ggttctggcc
     1081 ggacacggtg gcgaggtcac acgcgcgaaa ttcagtcccg acggccagca cgtgctgacc
     1141 gcctcattcg acacgacggc gcgtgcctgg agtgtcggcg gctcctctgt gggggcgccg
     1201 aaatagggcg cggcgtatcg cgcagggccg cccgtctcga cgccggccct gcgctcgcct
     1261 attgaggggc gatgcagcag aagcgcgacc aattcctggc cctcatcgcc gcgcgatcgg
     1321 caattatggg cattctaaac gtcacgccgg attccttttc cgacggcggc aaatttcagt
     1381 cgcacgatgc cgctatcgcc cacgcgcgga agctgaccgg cgagggtgcc gacatcatcg
     1441 atgtcggagc ggaatcgacg cgccctggcc atgtgcccgt cccggttgag gaagagcttg
     1501 cgcggctcaa gccggtgctg gcggatatcg tgaacgcgac cccgctgcct gtctcgatcg
     1561 atacgtcgaa ggccccggtg gcgcgcctcg cgattgagat gggcgccgcc gtcgtcaacg
     1621 atgtgtgggg gttgcagcgc gatccggcga tggccgatgt cgtggccgct ggcggcgcgg
     1681 cggctgtcgt catgcacaat cgcgaaaaca tcgatccggc gctcgatata ttgagcgaca
     1741 tgcggcgctt tttcgatcac tcgctcgaac tggcggagaa agccgggatt ccccgccgct
     1801 atctgatcct tgatcccggc atcggcttcg gcaagaccaa ggatcagaat ttgatcgcct
     1861 tgcgccgcct cgatcatttc aaggattacg gcttgccgtt gctcgtcggc gtgtcgcgta
     1921 aaagcctgtt cgcgccgatc ctcggcgccg ccgtcgatga gcggatgatc ggcactgtcg
     1981 cgacgaatat cattgccgcg gcgcgtggcg cctcgatctt tcgcgtccac gatgtcgccg
     2041 agcatgtcgc tgccttcaaa gtgtttgatg cgatcgagcg caacccggcc gccgcactat
     2101 gagtgagacc ggcctcgtag aaatcggcct tgggcttggc agcaatatcg gcgatgcgcc
     2161 gggcaatgtc cgcgaggcat tgcggctcct gcgggcgagc gatgccatcg aaattacgaa
     2221 agtctcgtcg atctacagaa ccgcgccgtg gggctataaa gatcaggcgg cgttcgcgaa
     2281 cgcctgcgcc ctggggcgca gtaagctcaa tccggaaagg cttctcgcgt cggtcaaagc
     2341 gatcgaggca gagatgggcc gcaccaagac ggtacgctgg gggccgcggc tgatcgatat
     2401 cgatattttg ttctacggca cgcaatcgct tgtgtcggat agtctggttc tgccgcataa
     2461 ggaactgttc aaccgcgcct tcgttctcgt tcccttgacc gagattgcgc ctgatctggt
     2521 gctgggcggg cgtccggttg ccgaggccgc cgcggcgatc aggcatgacg acatcgaaaa
     2581 atgggatccg tgataatggg caagcagatt aaactgacgg ccaaggacgg cgtgacgat
//
LOCUS       DASWOG010000591         6208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_14736,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000591 DASWOG010000000
VERSION     DASWOG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14736"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..812)
                     /locus_tag="VGB93_12025"
     CDS             complement(9..812)
                     /locus_tag="VGB93_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0146848.1"
                     /translation="MTRKILITGAGSGFGEGAAIGLARLGHEVIAAAHLWPQVTALRL
                     KAKELGLPSLRIEKLDLLDAYDVKQAAGWDFDVLVNNAGIGEGGPIAEIPLDIVRKNF
                     EINVFAPLVLTQQVVKKWVAAGTHGKIVFVSSMGGLFSPPGFSAYAATKHALEAIAEA
                     MYGELQPFGIQVQTINPGAFLTGFNEAMAENAFRWLDDDINFTKRDAMRKLVADLIGG
                     PGGRLDPEDMINKMIEVIPAEQGHFRNVFPTVIEDALKKHQAEMFARKI"
     gene            complement(829..1233)
                     /locus_tag="VGB93_12030"
     CDS             complement(829..1233)
                     /locus_tag="VGB93_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0146849.1"
                     /translation="MSVSLDQAEALVKTARAKAAEIGVAASIVVLDEAGHLKAFARME
                     HAWLGSIDVAMKKARTSVLFQMETQQVLEVCKPGAQAQGLELTNDGLVTFAGGIPLKT
                     SGGQLIGALGVSGGQVAQDFEIARAGLSALSA"
     gene            complement(1270..1692)
                     /locus_tag="VGB93_12035"
     CDS             complement(1270..1692)
                     /locus_tag="VGB93_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0146850.1"
                     /translation="MKTAKELMLAYLAGNAEQSGALFAEQGTLELPYLASINIPPVNK
                     GPEETTKFLTFLHNTLYPDFKFENIKVHLETPDQVFAEYFINHASGINGKQVHQQFFG
                     HLEAENGKIKRLREAIDVVVAAEAIYPNGLADVVAKES"
     gene            complement(1878..3941)
                     /locus_tag="VGB93_12040"
     CDS             complement(1878..3941)
                     /locus_tag="VGB93_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0146851.1"
                     /translation="MGEPNVDIFDLVEESVAVFGLDLRVNDWNREAQRLYGWTRGDVV
                     GGVIQSFVNCSPSEPLTQILACLQKTGTWRGEISRRTKSGGSVIVQVKWTLRRDASGA
                     DLDIVETSRDITEIKQKEEALDRVQYQYQNLFQASVAAFWEIEFSDARAMVHELTASG
                     VKNLREYFSAHPDFVRQMIRSTRIVDANEQCIATFGGDDREPLLRSLDPFWPDESLGI
                     FAESVVAFLEGRSHYSSEAVLYTIDGHRLDTIFTVSYPARLSPSARVLVGILDLTESK
                     LSKAAQEFTERRHQNFFHFLPVPLLRLDGSKAVEIVSRFKKQGISDFRQYLRDHPDVL
                     IDILEGQQIVEVNQRAVEVLRGRSVEEFKGSIKRYWMESLDAYREVVVARYEGKRGYE
                     ALLKLVAHDGTLLDVLFFAAFAPVTGAESVSLVGLIDVTDRVKAQEMLAHVQAEMAHA
                     ARVSVLGELTASIAHEVNQPLTAIATNTEASLLWLAHSPPNLDEIRDLSIRTAAEVQR
                     AADIIHRIRSMALRASPQYKSVDVNDIVEEAMLFLRHELQRNEVVSSLKLGDDLPSLR
                     GDPVQLQQVIVNLAVNAMQAMVQSERRPREIQVTTSIAEDDYVRLEVADSGGGIPETV
                     FARLFESFFTTKANGMGIGLPICRSIIEAHGGEIRAANKAANAGACFTVLLPSRESGA
                     ELRPV"
     gene            complement(4010..4492)
                     /locus_tag="VGB93_12045"
     CDS             complement(4010..4492)
                     /locus_tag="VGB93_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0146852.1"
                     /translation="MTGKRIERHLRSTNVWLVANDGGAMVRPGLAESSAWAIIVSGAI
                     ISIIDDDLATRSALATLMRAKGFDARAYESAEDFLDAGAQDNSRCIITDIHMTGMSGI
                     DLKLRLNEGHCMVPVIMITARVEDRLHERARDVGAFCLLRKPFKAEALIACIERALQP
                     "
     gene            complement(4492..5127)
                     /locus_tag="VGB93_12050"
     CDS             complement(4492..5127)
                     /locus_tag="VGB93_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146853.1"
                     /translation="MAQPGLVHIIDDDDGVRAALASLIRSVGNDARLYSSAAEFLASA
                     LPSVPSCLLLDVRLPEINGLDFQDSLRRRGIFLPVILMTGYGDIQMSVRGMKAGAVDF
                     LTKPVRHQDLLDAISTALARDREWRQDHAEVSVIRDRYSALTRRELEVFTLVTAGKMN
                     KQIAGDLNLSEITVKIHRGSVMRKMGARTLADLVKMAELLRMAGSGSNDHR"
     gene            5472..6161
                     /locus_tag="VGB93_12055"
     CDS             5472..6161
                     /locus_tag="VGB93_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0146854.1"
                     /translation="MKVLFIISSSDTAFWLSEVTHPYWHLTERGVEVDFASPAGGKVV
                     YDPYSNPYFEQSMESEDLVSKGFLSDKELVAKLDETLKLRGVDLDAYDAVHVAGGRGA
                     TFDLYPNEDVAAVLEHFWSKDKVVGAICHGAIALGNIPARIRGRRTTGYTLEGDLDLQ
                     RIFGSGFLIPHYPQTVLEDVGSIYSRVGANDPYVVKDGKLVTGQNQQSASEYALTLLH
                     TMTGVSPVAVK"
BASE COUNT         1339 a         1850 c         1681 g         1338 t
ORIGIN      
        1 ctaagagttc aaatcttgcg ggcaaacatt tcggcctgat gcttttttag cgcgtcttcg
       61 atgacggtcg gaaacacatt gcggaaatga ccttgctccg cagggatcac ctcgatcatt
      121 ttgttgatca tgtcctcggg atcgagacgc ccgccagggc cgccaatcag atctgcgaca
      181 agcttccgca tcgcgtcacg cttcgtgaaa ttgatatcat cgtcgagcca acgaaatgcg
      241 ttctccgcca tcgcttcgtt gaagcccgtc agaaacgcgc cagggttgat ggtctgtact
      301 tgaatgccga aaggctgcaa ttccccatac atcgcctcgg ctatagcttc gagcgcatgt
      361 tttgtcgcgg cataggccga aaaccccggc gggctgaaca gaccgcccat cgatgaaaca
      421 aaaacgatct tcccgtgcgt gcctgccgcg acccatttct tcacgacctg ctgcgtcaat
      481 accaatggcg caaagacatt gatctcgaaa ttcttgcgga caatatccag cggaatttcc
      541 gcgatcgggc cgccctcgcc gatgcccgca ttattgacga gaacatcgaa gtcccatccc
      601 gccgcttgct tcacgtcgta ggcgtcgagc aaatccagct tttcgatgcg caacgacggc
      661 aatcccaact ctttggcctt gaggcgcaag gcggtgacct gcggccacaa atgcgccgcc
      721 gcgataacct cgtgtcctaa acgcgccaag ccgatcgcgg cgccctcgcc gaaaccagag
      781 ccggcgccgg taatcaagat ttttctcgtc attttccgct ccaaaaaatt acgcgctgag
      841 tgccgacagt ccagcgcgcg ctatctcaaa gtcctgcgcc acctgcccgc ccgaaacccc
      901 gagggcccca atcaattggc cgccgcttgt cttcagcgga attccgccag caaaagtcac
      961 gaggccatca ttcgtcagct caagaccctg cgcctgcgcg ccgggcttgc acacctccaa
     1021 gacctgctgc gtttccatct gaaaaaggac gcttgtccgc gccttcttca tcgcgacgtc
     1081 aatcgagccg agccacgcgt gctccatgcg cgcaaaagct ttaagatggc ccgcttcatc
     1141 cagtacaacg attgacgccg cgacgccgat ctcggccgct ttcgctcttg ccgtctttac
     1201 gagtgcctct gcctgatcca aggaaaccga cataccatct ctccccgcgt ttccgataag
     1261 agatgggtgt caggattcct tggcgacgac gtcggcaagc ccattcggat agatcgcctc
     1321 ggctgcaacg acgacgtcga tcgcctcgcg caagcgcttg atcttgccgt tctcggcctc
     1381 gagatgacca aaaaactgtt gatgcacctg tttgccgttg atgccagacg cgtgattgat
     1441 gaaatattct gcgaagacct gatcgggcgt ttcgagatgg accttgatgt tctcgaactt
     1501 gaagtccgga taaagcgtat tgtgcaggaa ggtcaggaac ttcgtggttt cctcaggccc
     1561 tttgttgacg ggcggtatgt ttatcgaagc gagatagggc agttctaacg ttccctgctc
     1621 tgcgaaaagc gcgccggact gttcggcgtt tccggccaga taagccagca tgagctcttt
     1681 tgctgttttc atgactctgt ctccgtccaa tgctgccgtt tggcattggt gagagcgtgt
     1741 catgacaggc gcagcgcgac tatcagatgg ctgtctaagc gtactcatac gtcaggatga
     1801 tcattttcgt gcagcgatgt gcttgcgaag ctgtctcccc gcaatcggct gaaagccctg
     1861 atacctaagt atgactttta tactggccgc agttcggcgc cggactcacg tgagggcagc
     1921 agcacggtga agcacgcgcc agcattagcg gctttgtttg ccgcacggat ctcaccaccg
     1981 tgcgcttcaa taatcgagcg gcagatcggc agaccgatgc ccattccatt tgctttcgtt
     2041 gtgaagaaac tttcgaaaag ccgcgcaaaa acagtctcgg gaatgccccc accgctgtcg
     2101 gcgacttcga gtcgtacata atcatcttcc gcgatgctcg tcgtaacctg aatctcgcgc
     2161 ggacgacgtt cgctttgaac catcgcttgc atggcgttga cggcaagatt gacgatcacc
     2221 tgctgaagct gcacgggatc accgcggagg ctgggcagat catcgccgag cttgagcgag
     2281 ctcacaactt cattgcgttg taactcgtga cgtaaaaaca gcatcgcctc ctcgacgatg
     2341 tcattgacat ctacagactt gtattgaggg ctcgctcgca acgccattga gcggatgcga
     2401 tgaatgatgt cggcagcgcg ttgcacttcc gcggcggtcc ggattgaaag atcgcggatt
     2461 tcatcgagat tggggggcga atgagcaagc caaagcaggc tcgcttccgt attcgtcgca
     2521 atggccgtga gcggctgatt gacctcgtgt gcgatcgatg ccgttagctc tccgagcacg
     2581 gaaacgcgcg cggcatgcgc catctcggcc tgaacatgcg caagcatttc ttgcgctttg
     2641 acacggtcgg taacatcgat caacccgaca aggctcacgc tctccgcgcc cgtgacaggg
     2701 gcgaacgcag cgaagaacaa tacgtccaga agagtgccgt cgtgtgcgac gagtttgagt
     2761 aacgcctcgt agcccctctt gccttcgtag cgcgcaacga caacttcgcg gtacgcatca
     2821 agtgactcca tccaatagcg cttgatcgac cccttgaatt cctcgaccga gcgtccacgc
     2881 aagacttcga cggcgcgctg attgacctcc acgatctgtt gaccttccag gatatcgatc
     2941 aggacgtccg gatggtccct cagatattga cgaaagtcgc tgatcccctg ctttttgaag
     3001 cggctgacga tttcaacggc tttactgcca tcaagcctca acaacggcac gggcaggaaa
     3061 tggaagaaat tttgatgacg gcgttcggta aattcctgcg cggctttgga gagcttcgat
     3121 tcagtgagat caagtatgcc gaccagcacc cgtgccgacg gcgataagcg cgctggatag
     3181 gagactgtga aaatcgtgtc gagccggtgc ccatcgatag tgtagagcac cgcctccgag
     3241 gaataatggg accgtccctc caagaacgct acgacgctct cggcgaagat gccaagactt
     3301 tcgtctggcc agaacggatc gagactgcgc aacaaaggct ctcgatcgtc gccgccaaac
     3361 gtcgcgatgc actgctcatt cgcatctact atccgcgtcg accggatcat ttgcctgaca
     3421 aaatctggat gagccgaaaa atattcacgg agatttttaa cgccggacgc cgtcagttcg
     3481 tggaccatgg ctctggcgtc cgagaactcg atctcccaga aggcagcgac cgaagcctgg
     3541 aacaggtttt gatattggta ttggacccta tctagcgcct cctccttttg tttgatctcg
     3601 gtgatatcgc ggctcgtttc cacgatgtcg agatcagcgc cgctggcgtc ccggcgcagc
     3661 gtccatttca cctggacgat gacactaccg ccgctcttag tccggcgcga aatctcaccg
     3721 cgccaggtac ccgttttttg caggcaagcc agaatctggg tcagcggctc ggagggcgag
     3781 caatttacga agctctggat gacgccgccg acgacatctc cgcgggtcca gccatagagc
     3841 cgttgcgcct ccctattcca atcgttgaca cggagatcga gcccaaaaac ggccacgctt
     3901 tcctcgacca gatcgaaaat atcaacattc ggttcgccca cggggccgct ccctgtgggc
     3961 ggttcagagg aacgcgcggg cactcagtca aactccatgc gccgacccct cagggctgaa
     4021 gcgcacgctc gatgcatgcg atcagggctt ctgccttaaa cggtttgcgc agaaggcaaa
     4081 acgccccgac atcgcgcgcc cgctcatgta ggcgatcttc tacccgagca gtaatcatga
     4141 ttaccgggac catgcaatga ccttcgttca atcgcaactt gagatcgatc ccgctcattc
     4201 cagtcatatg gatgtcggtg atgatgcagc gcgaattgtc ctgcgcgccg gcgtccagga
     4261 aatcctcagc cgattcgtac gcgcgcgcgt caaagccctt ggcgcgcata agcgtcgcca
     4321 gcgctgagcg ggtcgccaga tcgtcatcaa tgatggagat aattgcgccg ctcacgatga
     4381 tcgcccatgc cgaggactct gcaagtcccg ggcgtaccat cgcacctccg tcattcgcga
     4441 caagccatac attggtgcta cggaggtgac gttctatcct cttgccggtc atcaacggtg
     4501 gtcgtttgat cccgagccgg ccattctcag caattccgcc atcttcacaa gatcggcgag
     4561 ggttcgggcg cccatctttc gcatgacgga gccacggtga atcttcacgg tgatctcgct
     4621 gagattgaga tcgccagcaa tctgtttgtt cattttcccc gcagttacga gcgtgaacac
     4681 ctcgagttcg cgtcgtgtca atgcggaata gcggtcgcgt attaccgaga cctccgcgtg
     4741 atcctgccgc cattcgcggt cgcgcgcgag tgcggtggaa atggcatcga gaagatcctg
     4801 gtggcggacg ggcttggtca ggaaatcgac agcgcctgcc ttcatccccc gtaccgacat
     4861 ctggatgtcg ccatagcccg tcatcagaat aacgggcagg aagataccgc gtctgcggag
     4921 cgagtcctgg aaatcgagac cattgatttc cggtaaacgc acatcgagca ataagcacga
     4981 aggcaccgaa ggaagcgcgc tggcgaggaa ttccgccgcg gaactataaa gcctcgcatc
     5041 attgccgacg gaacggataa ggctcgctag agcggcccgt accccgtcat catcgtcgat
     5101 gatatgaacc aatcccggct gtgccatacc tggaacatat cgaccgcgat cttcggcggc
     5161 aacaaggtca gaccactgca gcatcatcgt ttccccgttc aggcgtgcat atggcggcgg
     5221 cgcgatgcat ctctcggccg ggctgcacca tgataaggag aattggcccg gcaaaagagc
     5281 aatagaacat cagttttgcc ggcccatact ttcgtatagt gccgccgctc ccgcttgctc
     5341 acctgaaggt atagtgtttt gcagcctctg atctgatggt gggcgctctc tccgctggct
     5401 agcttgtcac aagttcaggc gccaagcatg gcatccaagc atggcgccga acggcaaaac
     5461 gggagcccgc catgaaagtt ctgtttatca tttccagctc tgacaccgct ttctggctgt
     5521 cggaggtcac acatccttat tggcatctga cggagcgcgg cgtcgaagtt gactttgcca
     5581 gcccggccgg cggcaaggtt gtttatgatc cctacagcaa cccctatttc gagcagtcaa
     5641 tggagtcgga agacctggtc agcaaaggtt tcctgtcgga caaggaactc gtcgccaagc
     5701 tcgacgaaac cttgaaacta cgaggcgtcg atctcgacgc ctatgatgcc gtccatgtgg
     5761 ctggcggccg cggcgcaacg ttcgatcttt acccgaacga agatgtggcg gccgttcttg
     5821 agcatttctg gagcaaggac aaggttgtcg gcgccatttg ccacggcgcg attgcgctcg
     5881 gcaatattcc agcgcgcatc cgcggccggc ggacgaccgg atacacgctg gaaggcgacc
     5941 ttgatttgca gcgtattttc ggcagcggct ttcttatccc gcattatccg cagaccgtcc
     6001 tcgaagacgt cggatcgata tattcgcgcg tcggcgccaa cgatccctat gtcgtaaagg
     6061 atggcaagct ggtcaccggg cagaatcagc aatcagcatc cgaatacgcc ctcacgctgc
     6121 ttcatacgat gacaggggtg agccccgtcg cagtgaaata aacgcgcttc cgatcgaaaa
     6181 cgctcaggac gatggagata tcgatgct
//
LOCUS       DASWOG010000592         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_87263,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000592 DASWOG010000000
VERSION     DASWOG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87263"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VGB93_12060"
     CDS             complement(<1..1775)
                     /locus_tag="VGB93_12060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0146855.1"
                     /translation="MTHDTQNPLLEDWTGPFGLAPFAAIKPDDFPPAFAQAFAAHKAE
                     IAAIADDPANPTFENTIVALERSGQFLSRIASVFFNLTGADTSEAIEAIERDVAPLFA
                     AHESEIHLNDVLFARIDTLWRQRETLGLDPEQQRALDRHHIMFRRAGAGQSRDAKARL
                     AEIGERLATLGTQFSQNVLGDENAFALILDEGDLDGLPDWQRAAAAEMATQRGHEGKY
                     AVTLARSSIEPFLQMSSRRDLREQAFRAWVARGENEGPSDNRAIAAEMVKLRAEKARL
                     LGYASYADYRLADMMAKSPAAAQGLLDEVWTRGRARALREQAALQELVTAEGNFTLQP
                     WDWRYYAEKRRKAAFDLDEAAFKPYLPLDQMIAAVFFTAQKLFGPSFEERTDIALYHP
                     DARVWTVRDATGRDIGLFIGDYFARASKHGGAWMGAYRDQQKLNGEIQPIIVNVLNFV
                     KPPPGEACLLSFDDARTLFHEFGHALHGLLSDVTFPSLAGTNVSSDFVEFPSQLYEHW
                     FEQSEILRRFARHHQTGEPMPEALIQKVIAARQFNQGFLTVEYTASALVDLALHSASD
                     FVEFPSQLYEHWFEQSEILRHFARH"
     gene            complement(1829..2757)
                     /gene="mepA"
                     /locus_tag="VGB93_12065"
                     /pseudo
     CDS             complement(1829..2757)
                     /gene="mepA"
                     /locus_tag="VGB93_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
     gene            2976..3566
                     /locus_tag="VGB93_12070"
     CDS             2976..3566
                     /locus_tag="VGB93_12070"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0146856.1"
                     /translation="MRKSAGVFLALVGVFLLSPAQARVTIDIDLSAQSMHVEGSGGTY
                     DWPISSARDGYATPDGTFSVQSLQTIHYSRKYYHSPMPHSIFFDGGYAIHGTYETAWL
                     GRPASHGCVRISPQNAATLFEMVKEEGATIHITGQPPETHYASVRHRHRIYAAAPRTW
                     GAQAPSYSGGWGYAPQRPQWGENPYGQEQNYYGEDQ"
     gene            complement(3574..>3687)
                     /locus_tag="VGB93_12075"
     CDS             complement(3574..>3687)
                     /locus_tag="VGB93_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY0146857.1"
                     /translation="ISREAQALKWVRPQNLRDYPMPPADAPLIPALLDLLT"
BASE COUNT          744 a         1135 c         1131 g          677 t
ORIGIN      
        1 taatggcgcg cgaagtggcg caggatttcg ctttgctcga accagtgctc gtaaagctgc
       61 gaggggaatt cgacgaaatc cgacgcgctg tgcagcgcca gatcgacgag cgccgaggcg
      121 gtatattcga cggtgagaaa accctgattg aactgccgcg ccgcaatgac cttctggatc
      181 aaagcttccg gcatcggttc gcccgtctga tgatggcgcg cgaagcggcg caggatttcg
      241 ctttgctcga accagtgctc gtaaagttgc gagggaaatt caacgaaatc agacgacaca
      301 ttggttccgg ccagcgacgg gaatgtcacg tccgacaaca gcccatgcaa cgcatgtccg
      361 aattcgtgga acagcgtacg tgcgtcgtcg aaactcagaa ggcaggcttc gcccggcggc
      421 ggcttcacga aattcaatac attgacgatg attggttgga tctcgccgtt gagcttctgc
      481 tgatcgcgat aagcgcccat ccaggcgccg ccatgtttcg aggcccgcgc gaaataatcg
      541 ccgatgaaga ggccgatgtc gcgcccggtt gcatcgcgca cggtccaaac gcgggcgtcg
      601 ggatgataga gcgcgatatc cgtgcgttcc tcgaaactcg ggccgaagag cttttgtgcg
      661 gtgaagaaaa ccgccgcgat catctgatcg agcggcaggt aaggtttgaa cgccgcctcg
      721 tcgagatcga acgccgcctt ccggcgcttc tcggcgtaat agcgccagtc ccagggctgc
      781 agagtgaagt tgccttcggc cgtaacaagt tcttgcagcg cggcctgttc gcgcagggcg
      841 cgcgcccggc cgcgcgtcca gacttcatcg agcagacctt gcgccgcggc cgggcttttc
      901 gccatcatgt cggcaaggcg atagtcggca tagctcgcat aaccgagcag ccgcgccttc
      961 tcggcacgca atttcaccat ctcggccgca atggcgcgat tgtcgctcgg cccttcgttt
     1021 tcaccccgtg cgacccaggc ccgaaacgcc tgttcgcgca gatcgcggcg cgatgacatc
     1081 tgtaaaaacg gctcgatcga cgaacgcgcc aaagtgacgg catatttgcc ttcatgtccg
     1141 cgttgcgtcg ccatctcggc ggcggcggcg cgttgccaat ccggcaggcc atcgagatcg
     1201 ccttcgtcga gaatcaacgc gaaagcattc tcgtcgccca gcacattctg cgagaattgc
     1261 gtgccgagcg tcgcaagcct ttcgccaatc tcggcgagcc gcgccttcgc gtcccgcgat
     1321 tgccccgccc cggcacggcg gaacatgatg tgatggcgat caagcgcgcg ctgctgctct
     1381 gggtcgaggc caagcgtttc tcgttgccgc cagagcgtat cgatccgcgc gaacagcaca
     1441 tcgttgaggt ggatttcaga ctcatgcgcg gcaaaaagcg gtgcgacatc gcgctcgatc
     1501 gcctcgatcg cttcactggt gtccgccccg gtgagattga agaagacact cgcgatccgg
     1561 ctgagaaact gacccgagcg ctccagcgcc acgatcgtgt tctcgaatgt cgggttcgcc
     1621 ggatcatcgg cgatcgcggc aatctcggct ttatgcgccg cgaacgcctg cgcgaaggcc
     1681 gggggaaaat catccggctt gatcgccgcg aaaggcgcca gaccaaacgg gccagtccag
     1741 tcttcaagca gaggattttg cgtgtcgtgc gtcatcggcc atccaaattg attgatccga
     1801 ctaatatggg cgtaccgccg ccatcgcgct acctctgtat cagaaccttg cggtaggtgg
     1861 cgggcagatc ggcaagtgtc agcgcgggcg cggccgcacc gggccaagct gcggacgcgg
     1921 atgcaatatg ccctccgaga accaataggc gagcgatgcg tgatcgcacc cgtcgcacgg
     1981 cggcacagga tcctgcccct cgcaagacgc ttcgccagcc ggacagacga gccggacatg
     2041 gaaataatcg tgaccgaaga acgggcgcac cttatgcagc caggcacgat ccgggccggc
     2101 ctcgcggcac aacgcctttt tgatcgccgc attgacgaag atgcgctgca cctcggaatc
     2161 ttccgccgcc gctcggatga cggcaagatg gctcggcgtc gaatagcgcg gatcgacatc
     2221 gagccggtcg gcccgcacaa cattggtgga catcatctcc tcgcgctgct cgcgcgagag
     2281 tgtgtaaggc ggcatcggtt tcagccagat atcgacatca agcccgatct gatgcgaggc
     2341 atggcccgtt atcattgggc cgccgcgggg ctgcgacatg tcgccgacga gaatgcccgg
     2401 ccagccgctg acgcgcggca cctcgcgcga aaagcgttca aggaatgaga caagctgcgg
     2461 cgtgccccaa ttgcgattgc gtgacagccg catgacctgc cagtccggcc cgttgacagg
     2521 cagcgcaata ccgccggcaa ggcagccgcg cgaataaaag ccgatgctgc gcgtcgctgc
     2581 ctcgctcggc gacaggactc ggccaaagag ctgtttggcg ggcgtcgagg ggccggcatg
     2641 agcaagcggc ggcagctttt tggcgtgcag cgtccccaga tcctgtgcgc tgccatcgac
     2701 agcggcgaag ccagcgacac tcacaattcc gagagcgaga accagaaacc tgcgcatatg
     2761 cgtacccctg caccggcatg tgccggcgac atatgtgcgc gactctgctt aacgctaact
     2821 atttacgacg agagaattcg tcgcgtcctc cgccgcgtta atgcggcgcc ccatcaacct
     2881 tacttgcaca caggagttta cgcagcgaag acactgtgac acgaatacag cattcctgtg
     2941 ataacgcggt gccatgtacc gcttggggat gcgtaatgcg taagtctgcc ggggttttcc
     3001 ttgcgctcgt cggcgtgttt ctgctgtcgc ccgcgcaggc cagagtcacg atcgacatcg
     3061 atctgagcgc ccagtcgatg cacgtcgaag gctcgggcgg tacgtatgat tggccgatct
     3121 catcggcacg tgacggttat gcgacgccgg atggcacgtt ctcggttcag agccttcaga
     3181 cgatccatta ttcgagaaaa tattatcact cccccatgcc gcattcgatc ttcttcgatg
     3241 ggggctatgc aatccacggc acttacgaga cggcgtggct cgggcggccg gcatcgcatg
     3301 gctgcgtacg gatttcgccg cagaacgccg caacgctttt cgaaatggtg aaggaagaag
     3361 gcgcgacgat ccacatcacc ggccagccgc cggaaacgca ttatgcctcg gtccggcacc
     3421 gccatcgtat ttatgccgct gcgccgcgca cttggggcgc gcaggcgccg agctatagcg
     3481 gcggctgggg ttacgcgccg caacggccgc aatggggcga gaacccatat ggccaagagc
     3541 aaaattatta tggcgaagac cagtagccgc gcgtcaggtt aatagatcga gcagcgcagg
     3601 tataagcggc gcatcggccg gcggcatcgg ataatcacgc agattttgcg gcctgaccca
     3661 tttgagggcc tgagcctcgc gcgagat
//
LOCUS       DASWOG010000593         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_124240,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000593 DASWOG010000000
VERSION     DASWOG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124240"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGB93_12080"
     CDS             <1..633
                     /locus_tag="VGB93_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0146858.1"
                     /translation="NGAAVSEMPFDWEARGRDFPRRNRIISGLANGVVIVEAAQRSGS
                     LITARFAAEQGREIFAVPGSPLDPRAEGANDLIRNGATLCTCADDVVEGLARQIADPV
                     RPHFLSENPGRPLEDHPLWDELDLPDVPRPPNSFIDEADADHRIRQTHQDDSAQARLI
                     ALLGPAPVSIDELARVANLSMRETRAILMEPELSGRLTRSGGDLISLTAN"
     gene            complement(1014..1295)
                     /locus_tag="VGB93_12085"
     CDS             complement(1014..1295)
                     /locus_tag="VGB93_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146859.1"
                     /translation="MRYLVIAAALSLVSPGVGFAQTSAPSTNPSAHSQPATSPNQPAG
                     SGMMEQGQGKPCSTQASGTSDRNGGNEKAATASGGSESNPNGKVSSGAC"
     gene            complement(1461..2825)
                     /locus_tag="VGB93_12090"
     CDS             complement(1461..2825)
                     /locus_tag="VGB93_12090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0146860.1"
                     /translation="MHPRHRQIGHKPHGNRFILTFAYHAHARTLAIPRFAAFLVFVLV
                     PIAAVIYLGATCYFIFHDDVLARLMQRQADMQYAYEDRIAALRRDVGNAQRRADAAAA
                     DFASRLVALSQRQDQVETRTALIAVFADHEKLMQLPAGTGDTAPDVAPASTSALPSPS
                     AEEAPAVAPEPDDGKPHPVDFDLRLRDHPHPVAMPFSTGDASPGAGGTPLSPDSQAHA
                     LAARYTHSDAIDLALLDDLAARPAQLNHDVRATLATLGLSASRFHIRTAKLSTDAGIG
                     GPFVPLPVMTDGSRFANEAAQLQNALATAEDLKSVLPRIPLNAPLPGDLEVTSPFGPR
                     IDPFLGRPALHTGVDLRGAYGDAVRATAPGRVTFAGAMGGYGNLVEVDNGNGLATRYA
                     HLSSIDVTQGEGVSTGSVVGHIGDTGRATGPHLHYEVRIDGQPVDPERFLRAGATLPT
                     ALKL"
     gene            complement(2897..3374)
                     /locus_tag="VGB93_12095"
                     /pseudo
     CDS             complement(2897..3374)
                     /locus_tag="VGB93_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            3411..>4049
                     /locus_tag="VGB93_12100"
     CDS             3411..>4049
                     /locus_tag="VGB93_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146861.1"
                     /translation="MSEPGPVATVSVFDKPCIFEYESAFQFSLLPKGHRGLVDHSDDI
                     GGGVRRAGEFPPAERDMRPPALLRSPLRPQFTLLVWLTQRLVGSITGLVLIVVLAVGS
                     FVIYEAKGPIAIGGLGTRIANALNARIGPGYAVKLGTVSVTTHGLGPTLSIENLTLIS
                     RGGATIITAPRAEVSVDLISLLYGKVVPKRLEVFGIEVRLILLPDGSLAVSAA"
BASE COUNT          768 a         1165 c         1264 g          852 t
ORIGIN      
        1 aacggcgcgg cggtcagcga gatgcccttt gattgggagg cgcgcgggcg cgattttccg
       61 cgccggaacc gaatcatctc cgggctcgca aacggtgtcg tcattgtcga ggcggcgcaa
      121 cgctcgggat ccctcattac cgcgcgtttc gcagcggagc agggacgcga aattttcgcg
      181 gtgcctggct caccgctcga tccgcgtgcc gagggcgcga acgacctaat tcgcaacggc
      241 gcaacgctct gcacctgcgc ggacgacgtg gttgaaggct tggcgcgaca gattgccgat
      301 cccgtgcgtc cgcattttct ttcggagaat ccgggccgtc ctttggagga ccacccttta
      361 tgggacgaac tcgatctgcc cgatgtgccg cgaccgccga atagttttat tgacgaggcc
      421 gatgcagatc atcgcatacg tcaaacgcat caggacgaca gcgcgcaggc gcgcttgatc
      481 gctctgctcg gtcccgcgcc cgtgtccatc gacgaattgg cgcgcgtcgc gaatttatca
      541 atgcgggaaa cgcgcgcaat cctgatggag cctgaactat cggggcgtct gacacgcagc
      601 ggcggagacc tcatcagttt gacggcgaat taaatttata aatttgcgac tccgcctcgg
      661 cgcgcgccat atccagaaaa tagttgagtg tacacaactg cgcgtcacca gccattttcg
      721 caagttcagc cgacatgttg gcgagacatg cgcggcatgc tgttgcgaga catcttggag
      781 agtcggcacg ggcgtttttt tggcgtccgt cggattcagc cgattttagc cgtcgcgtac
      841 gcgaaggttt ttggctgacc atagggactc cggaagcgta agcagaagcg atgcactcat
      901 aagttgtata agaatcgcct tctgtcagcg cagatttgca aaaatatctg cgcttacctc
      961 cgagtaatgc ccgcgccgtc ctctaacata atcgtgatca attttcagct ttatcagcaa
     1021 gccccggacg agaccttacc gttcggattg ctctcgctgc cgcccgacgc cgtcgcagct
     1081 ttttcatttc cgccgttccg gtcgctggtg ccgctggctt gggtcgaaca tggcttgcct
     1141 tggccctgtt ccatcatgcc ggaaccggcc ggttgattgg gcgacgttgc cggctgtgag
     1201 tgtgcgcttg ggttcgtgct cggcgcgctg gtctgcgcga agccgacacc cggcgaaaca
     1261 agcgacagag ccgctgcaat aacgagatat ctcatgttgt tcctcctgat tttatttcca
     1321 cctatccaat cgctgatagg ttcaggagtt cccgaacgga aaaggaggag ttcccgaggg
     1381 agttgattgg ttgaagttcg cgattgctaa gcggggctta ttcgcggaag cagcgccgcg
     1441 atcggcggct ttatgtggcg ctaaagtttt agcgcggtcg gaagtgtggc gccggcacgg
     1501 aggaaacgct cgggatcgac gggttggccg tcgatgcgaa cctcataatg gaggtgcggg
     1561 cctgtggcgc ggccggtatc gccaatatga ccgacgaccg aaccggtcga gacgccctca
     1621 ccttgcgtca cgtcgatact cgacagatgc gcataacggg tcgcaaggcc gttgccattg
     1681 tcaacttcga cgagattgcc ataaccgccc atagcgcctg caaacgtcac gcgtcccggc
     1741 gccgtggcgc ggacggcgtc gccataagcg ccgcgcagat caacgcccgt gtgcagggca
     1801 ggacggccaa ggaaggggtc gatgcgtggg ccgaagggcg atgttacttc aagatcccca
     1861 ggcagcggcg cgttgagcgg aatacgcggc agaacggatt tgagatcttc cgccgtggcg
     1921 agagcatttt gtaattgcgc cgcttcattg gcgaagcgcg agccgtcggt catcacgggc
     1981 agtggcacga aaggtccgcc gataccggcg tccgtcgaga gtttcgcggt cctgatatga
     2041 aagcggcttg ctgacaagcc gagtgtcgca agcgttgcgc gcacgtcatg attcaattgt
     2101 gctggccgcg ccgccaaatc atctagcaag gcgagatcaa ttgcatcgga atgggtataa
     2161 cgcgcggcaa gcgcatgcgc ctggctatcg ggagagagcg gcgtgccgcc agcacccggc
     2221 gacgcatcgc ctgtgctgaa gggcatggct accggatggg gatgatcgcg cagcctcaga
     2281 tcgaaatcaa ccgggtgcgg tttgccatcg tccggttccg gcgcgacggc gggcgcttcc
     2341 tccgcggaag gtgagggtag cgcggacgtc gacgcagggg cgacgtcggg tgccgtatcg
     2401 ccggttccgg cgggcaattg catcagcttc tcgtgatcgg cgaacacggc gatcagcgcc
     2461 gtgcgagtct cgacctgatc ctgccgttgc gaaagtgcga caagccgcga agcaaagtcg
     2521 gcggccgcgg catcggcacg gcgttgggca ttgcctacgt cgcgccgcag cgccgcgatg
     2581 cgatcttcat aagcatattg catgtcggct tggcgctgca tcagccgcgc cagcacgtca
     2641 tcgtggaaaa tgaaatagca ggttgcgccg agatagatca ccgctgcgat cggtaccagg
     2701 acaaagacca gaaacgcggc aaagcgcggg attgccagcg tgcgcgcgtg cgcatgatag
     2761 gcgaaagtca aaataaatcg gttgccgtgc ggcttgtggc cgatctgacg gtgacgggga
     2821 tgcatcaacg gacccaaccc agagcgagaa gaggccttga gcacacatcg ttaaggttaa
     2881 tattccttga aaccgctcag gttttcgtga gcgcttgcgc cgcagccagc acggcggcgg
     2941 cgtggccggg gactttgacc ttgtgccaga tattggcgat tttgccgtct ggcgattaaa
     3001 aaggtcgtcc gttcgacgcc catatatttg cgcccataaa ggctcttttc cgtccagacg
     3061 ccgtaggctt caagcatggt tttggattca tcggagatca gttcgatatc aagcttgtgc
     3121 ttggtcttga acttggcgtg actcgtggtc gaatcgggcg aaacgccgac aatggccgcg
     3181 cctgcggtcg cgaaggcgtc tttcagccca ttgaaatcaa tggcttcttt ggtgcagccc
     3241 gtcgtgtcgt ccttggggta gaaataaagc accaggctct ggccagcgaa ggatttcagg
     3301 ctttttatcc cacctgaatc atccggaagt ttgaaatccg gggctttgtc gccaggggca
     3361 agtttcttgc ccatctccgc cttcctgtcg tctcattaaa cggcaaacgc ttgagcgagc
     3421 cgggccccgt cgcgaccgtg tcggtcttcg acaagccatg tatattcgag tacgaatcgg
     3481 ctttccagtt cagcctgttg ccaaagggcc accgcggttt ggtcgatcac agcgacgata
     3541 ttggcggcgg cgtgcgccgg gcgggggaat ttcccccggc cgaacgcgat atgcgcccgc
     3601 cggccttgct gcgcagtccg ctccgtccgc aattcacgct tctggtctgg ctcacgcaac
     3661 gtctcgtcgg ctcgatcacg ggcctggtgc tgatcgtcgt tctcgcggtc ggttctttcg
     3721 tcatttacga ggcgaagggg ccgattgcga tcggcggcct cggcacccgt atcgccaatg
     3781 ctctgaatgc caggatcggc ccgggctacg ccgtcaaact tggtactgtg tccgtcacca
     3841 cgcatggtct cgggccgacg ctttcgatcg aaaacctgac cctgataagc cgtggcggcg
     3901 ccacgataat cacggcgcct cgcgccgaag tttcagtcga tctcatctcg ctgctgtatg
     3961 gcaaggtggt gccgaagcgt ctcgaagtct tcggtatcga agtgcggctt atccttttgc
     4021 cggatgggtc gctggcggtg tcggccgcc
//
LOCUS       DASWOG010000594         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_69153,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000594 DASWOG010000000
VERSION     DASWOG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69153"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VGB93_12105"
     CDS             <1..916
                     /locus_tag="VGB93_12105"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY0146862.1"
                     /translation="GPDHDRPELVTPDLLAALDRLTPLAPLHVPHNLAPIRAIAIVRP
                     KLAQVACFDTAFHHTMPIVATRFALPREYEAAGVRRYGFHGLSYEYIARQLRGVAPKI
                     AQGRVIAAHLGNGASVCAMQDGRSIDTSMGFTALDGLVMGTRCGTIDPGVILYLEEER
                     GLSPKEVEDILYRRSGLLGVSGGISCDMRTLLASEDLHAKEAIDLFVYHIAREIGALT
                     SSLGGLDGLVFTAGIGEHAPEIRARACALLKWLGAEIDPEANNRNAALISSPGSRVSI
                     HVIPTDEEAMIAQHAIETISASAPVARS"
     gene            947..1747
                     /gene="fabI"
                     /locus_tag="VGB93_12110"
     CDS             947..1747
                     /gene="fabI"
                     /locus_tag="VGB93_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0146863.1"
                     /translation="MANVTEAPATTPLVDLRGKRGLVIGIANEHSIATGCAEAFSKAG
                     ARLAATYLNARAEPFIRPVTNELGCELVLPCDVREPGQLEDVFAQVKAKWGGLDFLLH
                     SIAFAPKDDLHGRVTDCSLEGFDIAMNISCYSFIRMAHLAESLMQDGGCLLAVTFYGS
                     ERVVENYNLMGPVKAALESSMRYVAAELGPKGIRAHAISPGPIRTRAASGIDRFDDLL
                     ASAAARAPEHHLVDIADVGNLAAFLVSDAARRITGTIIPVDGGEHLAA"
     gene            complement(1770..>2390)
                     /locus_tag="VGB93_12115"
     CDS             complement(1770..>2390)
                     /locus_tag="VGB93_12115"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY0146864.1"
                     /translation="KETIDNLKSCSGALARNSNRVDGIMAGLERMAGGGPAEKPKPVY
                     DLTVSLPASSKLHGQLAIVEPTTVVALDTQRMLSLSSDGQISQIGDAQWSDSLPKLVQ
                     AKIVQSLENAGLNAILAEAADGPATGNQLQVDLQNFSMTGGASPIADVTFAAKIVAAD
                     GHVVGMRLFHATAPANGSDTPALVAAFSRAFSEAVGSLITWIAATI"
BASE COUNT          433 a          702 c          738 g          517 t
ORIGIN      
        1 cggcccggac catgaccggc ccgaattggt gacgcctgat ctccttgccg ctcttgatcg
       61 tctgacgccg ctcgcgcccc tgcatgtccc gcacaatctg gcgccgatcc gggccattgc
      121 cattgtgagg ccgaaactcg cgcaagtcgc gtgttttgat actgcctttc atcatacgat
      181 gccgattgtc gcgacccgct ttgcgctgcc gcgcgaatac gaggcggcgg gcgtgcggcg
      241 ctacggcttt cacggtctgt cgtacgaata tatcgcgcga cagttacgtg gagtcgcgcc
      301 caagattgcg cagggccgcg tcatcgccgc gcatctcggc aatggcgcga gcgtctgtgc
      361 catgcaggat gggcgcagca tcgacacaag catggggttc actgccctgg acgggctggt
      421 gatggggacg cgttgtggca ctatcgaccc cggcgtcatc ctctatctcg aagaagagcg
      481 cggattaagc cccaaagagg tcgaggacat tctctatcgc cggtccggcc ttctcggcgt
      541 ttccggcggg atttcctgcg acatgcggac tcttcttgcc agcgaagatt tgcatgcaaa
      601 ggaagcgatc gatctttttg tgtatcacat cgcgcgggaa atcggcgcgt tgacaagctc
      661 gctcggcggt ctcgacgggc tcgtgtttac ggccgggatc ggtgaacatg cacccgagat
      721 tcgcgcccgg gcctgcgcat tgctgaaatg gctcggggcg gagatcgatc cggaagcgaa
      781 caatcgcaat gccgcgttga tcagcagtcc gggcagccgc gtctccatcc atgtgatccc
      841 tacagacgaa gaggcgatga tcgcgcagca cgcgatcgag accatcagcg cgtccgcgcc
      901 ggtcgcgcga tcttgaatca gacatcaagc ccaaaggaat atccaaatgg caaatgtaac
      961 cgaagcgccg gcgacgacgc cgcttgttga tctgcgtgga aagcgcggcc tcgttatcgg
     1021 catcgccaat gagcatagta tcgcgacggg gtgtgccgag gcgttttcga aggccggcgc
     1081 tcggttggcc gcgacatatt tgaacgccag ggcggagccc tttattcgtc ccgtgacgaa
     1141 tgagctcggc tgcgaactcg tcctgccctg tgatgtgcgg gagcctggcc agctcgagga
     1201 tgtgtttgcg caagtcaaag ccaaatgggg tggtctcgat tttcttttgc attccattgc
     1261 tttcgcgccg aaggacgatt tgcatgggcg cgtcacggat tgctcgcttg agggtttcga
     1321 catcgcgatg aacatctcat gctattcatt cattcgcatg gcgcatctgg ccgaatcttt
     1381 gatgcaggac ggcggttgcc tccttgccgt taccttctac ggctcggagc gcgtcgtcga
     1441 gaattacaat ttgatggggc cggtgaaggc ggcgcttgaa agcagcatgc gctatgtcgc
     1501 ggccgaactc gggccgaagg gaattcgcgc gcatgccatt tcgccggggc ctatccgcac
     1561 acgcgcggcg agcggaatcg atcggttcga cgatttgctg gcctccgcgg ccgcccgcgc
     1621 gccggagcat catctggttg acatcgcaga tgtcggcaat ctcgctgcct ttctcgtcag
     1681 cgatgctgcg cgtcggatca ccggcacgat tattccggtg gatggcggag agcatctcgc
     1741 cgcttaagtg cgagtggccg cgcggagctt tagatcgttg ccgcaatcca ggttatgaga
     1801 cttccaacgg cctcgctaaa ggctctgctg aaggctgcca cgagggctgg cgtatcgctt
     1861 ccatttgccg gcgctgtcgc atggaacagc cgcatgccga cgacatggcc gtcagccgcc
     1921 acaattttgg ctgcgaatgt aacgtccgcg attggcgaag cgccgcctgt catcgagaaa
     1981 ttctgaagat cgacctgaag ttgattgccg gtcgcggggc cgtccgctgc ttccgccaat
     2041 attgcgttca gcccagcgtt ttcgaggctc tgcacaatct tcgcttgaac aagtttcggc
     2101 aatgaatcgc tccattgcgc atcgccgatt tgagatattt ggccgtcgct cgaaagcgaa
     2161 agcatccgct gcgtatcgag agcgacgacg gtcgtcggct cgacaattgc gagttggccg
     2221 tggagcttgc tggaagcggg aaggctcacc gtcagatcgt aaaccggctt tggtttttcg
     2281 gcggggccgc cgcctgccat gcgctcgagc ccggccatga tcccgtcgac gcgattggaa
     2341 ttgcgcgcca acgcgcccga acatgatttc aggttatcga tcgtttcctt
//
LOCUS       DASWOG010000595         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_179502,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000595 DASWOG010000000
VERSION     DASWOG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179502"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..621)
                     /locus_tag="VGB93_12120"
     CDS             complement(22..621)
                     /locus_tag="VGB93_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEY0146865.1"
                     /translation="MTKTDPFEREPGARVETVSILNEASEDKTLLVVDDDRAFLTRLA
                     RAMEGRGFIVTIAQSVAEGLAVIAHQPPAFAVIDMRLADGNGLDVISELKAKRPEARA
                     IILTGYGNIVTAVTAVKLGAFDYLAKPADADDIYAALMATHHDKAEVPENPMSADRVR
                     WEHIQRIYELCGRNVSETARRLNMHRRTLQRILAKRAPR"
     gene            complement(727..>1944)
                     /locus_tag="VGB93_12125"
     CDS             complement(727..>1944)
                     /locus_tag="VGB93_12125"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEY0146866.1"
                     /translation="FIAGSAWLKVVRCFRYNQNDLLEDKQAAAILGDDIIQLGALLYL
                     TGGIENPFAVLLLAPVMIAAVSFSPRTTGGLFVLMVVVATAISFFHYPLPWMQNSPFQ
                     IPALYSVGIWAAVTVSAAFTVVYASRIAEETRKLADAFAATELVIAREQLLSQLDGLA
                     AAAAHELGTPLATISIAAKELRKQLPSGDHDDDVGLIIQEVGRCRSILGKLASLGEDE
                     PGNILQEMNLDLLIEEAAGPYRGFDIAINVVKNGSAPEPVCRRNPGLLYGLGNLIENA
                     TDFAKSEVRVIARWNKNILEITIEDDGPGFSPDVIGRIGEPYVTTRADRHIKHEADSG
                     LGLGLFIAKTLLERSGATVVTSNRLPPQSGARITMRWDRARFESNALSAAALAARLAP
                     RPETGQISQRPAH"
BASE COUNT          365 a          587 c          616 g          376 t
ORIGIN      
        1 ccgatgcgtt aaaaaggcaa gtcagcgcgg cgcgcgtttg gcgagaatgc gctgcaacgt
       61 ccgccggtgc atgttgagcc ggcgtgctgt ctccgaaaca ttgcggccgc aaagctcgta
      121 gatgcgctgg atgtgctccc agcgcacgcg atcggcggac atcggatttt ccggcacttc
      181 ggccttatcg tgatgtgtcg ccatcagcgc ggcgtaaatg tcgtcggcat ccgcgggctt
      241 tgcgagataa tcgaaagcgc ccagctttac cgctgtcacg gcggtgacga tgttgccata
      301 gcccgtgagg atgatcgcgc gcgcctcggg ccgtttggcc ttcagttctg agatcacatc
      361 aaggccgttg ccgtcagcga gccgcatatc gatcacggcg aaggccggcg gctgatgcgc
      421 aatgacggcg agaccttcgg cgacagattg cgcgatcgtc acaatgaagc cgcgcccctc
      481 catcgctcgc gcgagtcgtg tcagaaacgc ccgatcgtca tcgacgacca gcagcgtctt
      541 gtcttcggac gcctcgttta aaatcgatac ggtttccacc ctggcccccg gttcccgttc
      601 gaaggggtct gttttcgtca taagtcagag cctcttggcc atgttaagcc ggtttggcgc
      661 gcgctagaat ggctaattcg ctttcttggc ctttttgccg atgccagagc gaaatggcaa
      721 tcaccttcaa tgggccgggc gctgcgagat ctgccccgtt tcaggacgcg gcgccagccg
      781 cgcggccaac gcggctgccg acaatgcgtt cgattcgaag cgcgcgcggt cccagcgcat
      841 agtaatgcgc gcgcccgatt gcggcggcaa cctgttgctg gtgacgaccg tcgcgccgga
      901 acgttcgagc agtgttttgg cgataaagag gccgagaccg aggcctgaat ctgcctcgtg
      961 cttgatatgc cggtcggccc gtgtcgtgac atagggttcg ccgatgcggc cgatgacgtc
     1021 tggcgaaaac cccggcccgt cgtcctcgat cgtgatctcg aggatgttct tgttccaccg
     1081 ggcgatgacg cgcacttccg acttggcgaa gtcggtggca ttctcgatga ggttgccaag
     1141 cccgtagagc aggccggggt tgcgccggca cacgggctcg ggcgccgagc cgttcttcac
     1201 aacgttgatc gcgatgtcga agccgcgata ggggcctgcc gcttcctcga tcagcagatc
     1261 gagattcatc tcctgcagga tattgccggg ttcgtcctcg ccgagcgaag caagcttgcc
     1321 gaggatgctg cggcagcggc ccacttcctg gataatgagg ccgacgtcgt catcgtgatc
     1381 gccggacgga agctgtttgc gcagctcttt cgccgcgatg ctgatcgttg cgagcggcgt
     1441 gccgagttcg tgcgccgcgg ccgcagctag tccatcgagc tgactcaaaa gctgttcgcg
     1501 ggcgatgacg agttcggtcg ccgcgaaggc atcggcgagc ttgcgcgtct cttcggcgat
     1561 tcgcgacgca tagaccactg tgaaggcggc gctcacggtg accgccgccc agatgccaac
     1621 actgtaaagc gccggaattt gaaacggcga attctgcatc cacggcagcg gataatgaaa
     1681 gaagctgatc gcggtcgcca cgaccaccat caggacaaag agaccgccgg tcgtgcgcgg
     1741 cgaaaacgag acggcggcga tcatcaccgg cgcgaggagc agaaccgcga agggattctc
     1801 gatccccccg gtgagataga gcaacgcccc gagctggatg atgtcatcgc cgagaatcgc
     1861 ggcggcctgc ttgtcctcaa gcagatcgtt ctgattatag cggaagcaac gcacgacctt
     1921 aagccacgcg gagccagcaa tgaa
//
LOCUS       DASWOG010000596        12613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_235295,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000596 DASWOG010000000
VERSION     DASWOG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235295"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..144
                     /locus_tag="VGB93_12130"
     tRNA            70..144
                     /locus_tag="VGB93_12130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Thr,seq:cgt)
     gene            397..828
                     /locus_tag="VGB93_12135"
     CDS             397..828
                     /locus_tag="VGB93_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146867.1"
                     /translation="MKKSYYLAAFALAATLGSVSTTGFAAGLDTASFQVDETSPADRA
                     PALGSRPFLVEHGATDPRVVSAVRVTAENPLADRAAALGGEAFTVKSAESRRYHTAPQ
                     TSAETDEATGNPLAERAATLGGKVYTVEHSVPSQSFLARFN"
     gene            complement(920..1390)
                     /locus_tag="VGB93_12140"
     CDS             complement(920..1390)
                     /locus_tag="VGB93_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0146868.1"
                     /translation="MGETATSLMPCNNGAIRQTARMLGQLYDDVLAPSGLRATQHTLL
                     YTIQVMNEPTLGEMAQAMVMDLSALGRSLKPLEREGLVAIVPDPSDGRAKRATLTKAG
                     LKTLKYTAELWRNAQDRFEKAFGAKRAEELRSVLSGLSSPEFREAFEQFSALKK"
     gene            1614..2465
                     /locus_tag="VGB93_12145"
     CDS             1614..2465
                     /locus_tag="VGB93_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0146869.1"
                     /translation="MATDNISAVNAPNLFIEAANGIRYAYRRFGKSGGVPLILMQHFR
                     GNLDFWDNALLDALAAEREIVAFDNTGVAATSGRTPDNVRQMARDAISFIEALKFETV
                     DILGFSLGGFVAQDVTLLRPYLVRKLILAGTGPQGGPGMHGWVKDIADAARKPDSGGA
                     ELAYIMFKGTPTSQAKGQEYLGRFMARQADRDAGGTLQTRDAQYDAVVDWGIPDHSKL
                     QRLAAIEQPTFVASGDDDRMLPPRLAYIMAGLIPNAQIKIYPDAAHGFLWQHHREFAA
                     DVNSFLG"
     gene            complement(2569..2781)
                     /locus_tag="VGB93_12150"
     CDS             complement(2569..2781)
                     /locus_tag="VGB93_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146870.1"
                     /translation="MIIVHAFKRWNHTKAEYDFPKFKRTAEAIKARQCVIIPDTEEKV
                     SAHMLDFQGRYDPALWLHLKSGARAQ"
     gene            complement(2968..3180)
                     /locus_tag="VGB93_12155"
     CDS             complement(2968..3180)
                     /locus_tag="VGB93_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0146871.1"
                     /translation="MTTGTVKWFNSDKGYGFIAPDDGSRDAFVHISALERAGINNLQE
                     GQKVDFDLAADQRSGKMSASNLKVLG"
     gene            complement(3451..4017)
                     /locus_tag="VGB93_12160"
     CDS             complement(3451..4017)
                     /locus_tag="VGB93_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0146872.1"
                     /translation="MNASKITLSAIALLSFSAENSFAHSRRHFSLTGGSLIYASYYSS
                     GYRTASGERFNPNGYTAAHRSLPFGTRLQVINPRTGRSVVVRINDRGPFVRGRSLDLA
                     RGAAFAIGMRGTAPVRIAVLGHGAVAVAARTTPIQTTSWYADQARVPRTHAANSWSNA
                     PAAHWPKVGGNLGRVLTASTPIQSTWRD"
     gene            4566..6296
                     /gene="poxB"
                     /locus_tag="VGB93_12165"
     CDS             4566..6296
                     /gene="poxB"
                     /locus_tag="VGB93_12165"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEY0146873.1"
                     /translation="MATYRVADLVIESLHATGVRHIYGVVGDSLNAITDALRKREDME
                     WIHTRHEETAAFAAGADAHLTGQLAVCAGSCGPGNLHLINGLYDCHRSRVPVLAIAAH
                     IPSAEIGRSYFQETHPESLFRECSHYCELVSDPSQLPGVLETAIRAAIGRRGVAVVII
                     PGDVALREAVGTASTSRALVANPPVVVPSEHDLDELAALLNASEKTTLFCGRGCAGAH
                     QALLQLADALKSPIVHAFGGKEYVEYDNPFDVGMTGLIGFSSGYHAMLDCDTLLMLGT
                     DFPYRQFYPTDAKIAQIDIRPENLGRRTRIDLGLIGDVNATIAALLPKLKVKEGRFHL
                     DESLAHYRKAREGLDDLAVAGPGDKQIHPQRLAKLVSEYASDDAVFTFDVGTPVVWAA
                     RYLKMNGRRRLIGSLNHGSMANAMLQAIGAQVAFPSRQIISLSGDGGFSMMMGDFLTL
                     VQHKLPVKIIVFQNQTLGFVALEMKAAGFLETGVSLAGVDFAAMARAIGVHGIRVDDP
                     ADLEDGVKNVLAHEGPALLDVRTATQELAMPPAIKLDQVKGFGLWALRAVMNGRGDEL
                     IEVARTHLRR"
     gene            complement(6366..7790)
                     /gene="fumC"
                     /locus_tag="VGB93_12170"
     CDS             complement(6366..7790)
                     /gene="fumC"
                     /locus_tag="VGB93_12170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0146874.1"
                     /translation="MVDVRRETDSLGVVEVPADKLWGAQTQRSLEHFSIGKDLIPREM
                     ITAYAILKKAAAGANHAGDRLDDRRHKLIVQTCDEILAGQHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCSQLAGTALGSHAPVHPNDHVNMAQSSNDAFPSAMYIAAAVGVKQRL
                     IPAVAALRDAIEVKSEAWSDIVKIGRTHMQDATPLTLGQEWSGYAGMLSEDLERIEAA
                     VQGVYRLALGGTAVGTGINSAPGFAEAAAAEIARLTGLPFITAPNKFTVQGAHDALVH
                     LSGTLRTLAVSLYKIANDIRLMSCGPRAGFAELEIPENEPGSSIMPGKVNPTQCEALT
                     MIAVQVMAADVAVGFGGAGGYLEMNVYKPLMIFNVAHSITILTDGCVNFRKFLIEGTK
                     PNLKKINEYVERSLMLVTALSPVIGYDKASKIAHYAMDNDLTLKDGALKLGFVTEAEF
                     DRLVDPKKMVKPYVASDASATSHG"
     gene            complement(7808..9304)
                     /gene="mqo"
                     /locus_tag="VGB93_12175"
     CDS             complement(7808..9304)
                     /gene="mqo"
                     /locus_tag="VGB93_12175"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEY0146875.1"
                     /translation="MSIVNEDNPDAVFIGAGIMSATLATVLKELEPSLTIMMFERLDD
                     CGQESSQAWNNAGTGHAANCELNYTPQRSDGSVDISKALEVNTEFDISRQLWTHLVRK
                     GAIPDPHAFIHACPHMSFVWGRENVAFLRARYKEMSAHHCYEGMEYSEDRTTIAGWAP
                     LIIDGRPEDQQIAATRMVTGADVDYGALTHLLVAQLSAEPGFAVRYNRQVVDLKRGGD
                     GRWRVTVEDTYDGTRQTASAKFVFIGAGGAALELLQKSEIPEGRGYGGFPVSGIWLRC
                     DVDAVSERHHAKVYGKAATGSPPMSVPHLDTRVIEGRKHLLFGPYAGFSSKFLKYGSL
                     TDLFESIRPDNIRPLLDVARDNFALSEYLIGQVLQSSSHQFALLQQFFPKAARRDWYK
                     AVADQRVQIIKPDKERGGVLEFGTELVAASDKSLVALLGASPGASTAAFIAQEVLEKC
                     FADKLTDDGWLPALKRMIPTYGIDLKIDAEACRLTRADTAPVLGLETV"
     gene            complement(9322..9714)
                     /locus_tag="VGB93_12180"
     CDS             complement(9322..9714)
                     /locus_tag="VGB93_12180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0146876.1"
                     /translation="MIRCVRMWTGDDGDSHFEEGLIALSSGERNDSLTEAVDAVNVSF
                     QETKSGGSYAWHKDPVPRFVITLSGVLEFETRGGETFVIRPGDILLAQDNTGSGHRWK
                     LIGEEPWRRVYVVYRPGVPLPFQALPKP"
     gene            complement(9901..9990)
                     /locus_tag="VGB93_12185"
     tRNA            complement(9901..9990)
                     /locus_tag="VGB93_12185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9954..9956),aa:Ser,seq:gga)
     gene            10165..10521
                     /locus_tag="VGB93_12190"
     CDS             10165..10521
                     /locus_tag="VGB93_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146877.1"
                     /translation="MANEATASDSPMFRFFLRLIGLLLLAGAFAALIVDGTRSIAAGA
                     LALTPLAQTLAWLSPQKFEALKPQLQHHVPHFVWDPLIVHLLTAPTWIVAGALGALVL
                     LITQKRRPKIGYSNRE"
     gene            complement(10558..11010)
                     /locus_tag="VGB93_12195"
     CDS             complement(10558..11010)
                     /locus_tag="VGB93_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0146878.1"
                     /translation="MARDDDLHDLTRHPIFAVLEPEALRFIALSAGQRILRSGEVLFS
                     RDEPSDGGYFLRSGCVVLEVGDRSREEKVIQPPALIGEMALIAPTRRPATAIAREPSG
                     VLQISRTLFQRALSESPRSARRLQQFLEKRLQGFAQELNALRENAFED"
     gene            complement(11023..11709)
                     /locus_tag="VGB93_12200"
                     /pseudo
     CDS             complement(11023..11709)
                     /locus_tag="VGB93_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            11762..12373
                     /locus_tag="VGB93_12205"
     CDS             11762..12373
                     /locus_tag="VGB93_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0146879.1"
                     /translation="MTNPRKQRGPGLSKARKGCGKPKVETRLRRIVATALPRFTPDAP
                     ARGRLHAGQTVFTCALGATAISHRKREGDRTSPGGHFRLIEGFFKPLIGRRPRTSLPI
                     CAISRELGWCDDPDSGNYNRLIRLPAQARHETLWRDDGLYDLVIVLDYNLSPRRKGAG
                     SAIFLHCARPGFTPTEGCIALRQEDLRKLLPRLARHAILIVQR"
     gene            12394..>12613
                     /locus_tag="VGB93_12210"
     CDS             12394..>12613
                     /locus_tag="VGB93_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146880.1"
                     /translation="MNILLCGRLAMLTLSGLTLSAAGVSAAPAPQTYCAKVVNDDELR
                     GAPHSLSPAIKQIFHIGGAYAAQTTRYRC"
BASE COUNT         2585 a         3797 c         3614 g         2617 t
ORIGIN      
        1 gagccctggc ggcggttgcc aaatcggccg caaaaggcat aagagaggcg cgcggtcatc
       61 tccgccgagg ccgcaatagc tcagccggta gagcccgtca ttcgtaatga cggggtcggg
      121 ggttcgaatc cctcttgcgc accatctgtt tctaatccat tcgccagaac aggttgctgg
      181 tttttgcgtt ccaatgatca ggcacgccaa agtccctatt cataaattgg gcggtaaatg
      241 ctcgcgctca gtcctcgtcg gaggtgacga gcggccaatc ttttttaatt ccgcttattg
      301 acaccgccct gtcagagaac catttccaag ccacgatgct ggtatataca agtaaatgac
      361 ttgcatgccg catcgatctg ctcaggagtc aatgcaatga agaagtccta ttatctcgcg
      421 gcgttcgctt tggccgctac gctcggcagc gtgtcgacta ccggatttgc agccggcctc
      481 gataccgcca gcttccaggt cgatgaaaca tcgcccgccg accgtgcgcc ggcgctcggc
      541 agcaggccat tcctcgtcga gcacggagcg accgacccgc gtgtggtgtc agcggtgcgt
      601 gtcacggctg aaaatcctct cgccgatcgt gcggcggccc ttggcggcga ggcttttacg
      661 gtgaagagcg ctgaaagccg ccggtatcat actgcgccac agacgagtgc cgagaccgac
      721 gaggctaccg gtaatccgct cgctgagcgt gcggcgacgc tcgggggcaa ggtctacaca
      781 gtcgagcaca gcgtgccgtc ccaatcgttc ttggcgcgct tcaactgaat gccggatatt
      841 ctgtgcgcgg tcaatttggc cgcgcatttt cgtcgcgggc taggcgctgc cagcgccggc
      901 gcgcgagccc gatgcagccc tatttcttca acgcagaaaa ctgctcgaac gcctcgcgga
      961 attccgggct cgatagaccg ctcaacaccg aacgaagctc ttccgcgcgt ttagcgccga
     1021 aagctttctc gaaacgatcc tgggcgttgc gccagagctc ggccgtatat ttcaatgttt
     1081 tcaagcccgc cttggtcagc gtggcgcgct tcgcccgccc gtccgaggga tcgggcacga
     1141 tggcaactag accctcgcgt tcaagcggct tcaatgagcg cccgagcgcc gataagtcca
     1201 tcaccatcgc ctgcgccatc tcgccaagcg tcggctcgtt catcacctgg attgtgtaga
     1261 gcagcgtgtg ctgcgtcgcg cgcagtccgc tcggcgcgag cacatcatca taaagctggc
     1321 ccagcatgcg ggccgtctgc cggatcgcgc cattgttgca tggcatgaga ctcgtcgctg
     1381 tttcgcccat cctaaaaatc cgctctcgat tgtctgcatg taatttatac gacaaaaaaa
     1441 aatattcaag gcatatgcaa ctgatttttc acgcggaagt cttcggctgc gcacgccaaa
     1501 cgtagaaact tcgcacagac cgacttgcca acacccgcgc ttcgtgtatt tacttgcata
     1561 tgctagttgt tagctgacag catgtttcaa tcgtttgcaa ataggaggaa ccaatggcca
     1621 ccgacaacat cagtgcagtg aacgcgccca atcttttcat cgaagccgcc aacggaattc
     1681 gctacgccta tcgccgtttc ggcaagagcg gcggcgtacc gcttatcctg atgcagcatt
     1741 ttcgcggcaa tctcgacttc tgggataatg ccctcctgga cgcgctcgcg gctgaacgcg
     1801 agatcgtcgc gttcgataat accggcgttg cggcaacgag cggcaggacg cccgacaatg
     1861 tgcggcagat ggcgcgcgac gccatttcat tcattgaggc actgaagttc gaaacggtcg
     1921 acatcctcgg attctcgctc ggcggattcg tcgcgcagga cgtcaccttg ctgcgcccct
     1981 acctcgtccg gaaactgatt cttgcgggga ctgggccaca gggtggcccc ggtatgcatg
     2041 gctgggtcaa ggacatcgcc gacgcggcgc gcaagcccga tagtggcggc gccgaactcg
     2101 cctacatcat gttcaaaggg acgccgacca gccaggcgaa aggtcaagag tatctcggcc
     2161 ggttcatggc gcgccaggca gatcgcgatg caggtggcac gctgcaaacg cgcgacgcgc
     2221 aatacgacgc cgtcgtggat tggggtatcc cggatcacag caagctgcag cgtcttgccg
     2281 ccatcgagca gccgacgttc gtcgcaagcg gcgatgacga ccgtatgctg ccgccacgtt
     2341 tggcctacat catggccggg ctgatcccca atgcgcagat caagatctat cccgatgccg
     2401 cgcacggatt tctctggcag catcatcgcg aatttgccgc ggacgtgaac agcttccttg
     2461 ggtgaaacaa acggcgcttc acgcgcgaat gcgcagcgac tcaatgcagc cgctgcgaag
     2521 tcaccttaaa tcagcgatgc cggagtaggg cggttcgcgc gcgggaattt attgcgcgcg
     2581 ggcgccgctc ttcagatgca gccaaagcgc tggatcgtag cgaccttgaa aatccagcat
     2641 atgtgccgag accttttctt cggtgtcggg aatgatgacg cattggcgcg cttttatagc
     2701 ttcggccgtg cgcttgaatt tcgggaaatc atattccgcc tttgtgtggt tccaacgctt
     2761 aaaggcgtga acaataatca tgaagtttcc cgtgcgctga agcggcttat cgaccaatct
     2821 gaaggaaagt ggcatgcggc gtgcccgaaa aattcaaggc cgccgccgcc gttcgacgca
     2881 gaccaaaccg ccagtacatc cgtggtcggt tcgatcttgc gaagagatat ttagccgccg
     2941 cgaattgcgc gaagcggcag ggcaaaatca gcccaaaacc ttcagattgc tggccgacat
     3001 cttgccgctt ctctggtcgg cggcgagatc gaaatccacc ttctgacctt cttgcagatt
     3061 gttgatgcca gcgcgctcga gtgcactgat gtgcacgaac gcgtccctgc tgccgtcgtc
     3121 cggcgcaata aaaccatagc ccttatcgga attgaaccac ttcacggttc ccgtcgtcat
     3181 gggaaaccct ttcgtaaaca tagataatgc ttgtccgcgg gaagccgcgg atcgtcgaaa
     3241 tcgcatattt tcaggagaga gaattcagct cggcgcggat attttctcgc gcgaggccaa
     3301 cttgtccggc cgtctatcga cgtgttcaac atataccgtt gccaaaagat atacaaggca
     3361 gcgtacggat gtttcacgct gaggtcgtaa agcacactcg tagcggccca tcgggccgct
     3421 acgagtaatc atgtcttgct cgcgccggcc ttaatcgcgc caggtcgact ggattggcgt
     3481 gcttgccgta aggacgcggc caagattgcc accaactttc ggccaatgcg ccgcgggcgc
     3541 attcgaccag ctattcgccg catgcgtccg cggcacgcgg gcctgatcgg cataccagct
     3601 cgttgtctga atgggcgtcg tccgagccgc gacagcgact gcgccatggc cgagcacggc
     3661 aatccgcacc ggcgccgtgc cgcgcatgcc gattgcgaaa gctgcgccgc gcgcaagatc
     3721 cagactacgg ccgcggacaa acgggccacg atcgttaatg cggaccacga cgctgcggcc
     3781 agtccgcggg ttgatcacct gcaagcgcgt gccgaacggc agcgagcgat gcgcggcggt
     3841 gtagccattg ggattgaagc gttcccccga agccgtccgg tatccggacg aatagtagga
     3901 tgcgtaaatc aacgacccgc cagtgagact aaaatgccgg cgagaatggg cgaaagaatt
     3961 ttcagcggag aatgaaagaa gagcgatagc agatagcgta atttttgatg cgttcaacgc
     4021 gtcacccttt cagaagttgc agaggggtgc gtattgattg tcataatgtg ttcataaatg
     4081 gcgattcatg cggcgatagt gagataaagc ttcggctgat tggccaaaac gtcgcagcaa
     4141 atatgtcaga actggcatcg ttccatcata tttcatggat gtgctggact tagactcatg
     4201 atcatctgaa aatttactat ttatttacca tgttattttc taattaaagc caaggctaag
     4261 attgctagtc agacgcccgc ctgttcagct cctgtttccg aagatcggcg catcagctcc
     4321 gagcagaaca agtttaggct cgcgcaattc ttcgccgatc ttgctccgtg caaccccccg
     4381 actctgaatt ttgttcgcat cctgacggct tcgcgtcaca ggaagccctc gaagccgcgc
     4441 gctcacgatc cgtttcgcga tcccgtttca tgggaataac aaatagacgc gtttcaatcg
     4501 gcagctaaac taccgccgct gcttcgggcg caaagccgaa cggcgctcgt tccggaggcg
     4561 tacccatggc aacgtataga gtcgctgatc tcgttatcga gagtctccac gccaccggcg
     4621 tgcgccatat ctacggtgtg gttggcgaca gtctgaacgc catcaccgac gcgttgcgca
     4681 agcgcgagga catggaatgg atccacacgc gccatgaaga aaccgcggct ttcgccgctg
     4741 gtgctgacgc ccatctcacc ggccaactcg cggtctgtgc cggcagctgc ggccccggca
     4801 atctgcatct gatcaacggt ctttacgatt gccatcgctc acgcgtccct gtgctggcca
     4861 tcgcagcgca tattccgagc gccgagatcg gccgcagcta ttttcaggaa acccatccgg
     4921 aatctctgtt tcgcgaatgc agccattatt gcgagcttgt cagtgacccc tcgcagctcc
     4981 ccggcgtgct tgagaccgcc attcgcgcgg cgatcgggcg gcgtggcgtt gccgttgtta
     5041 tcatcccggg cgatgtcgcg ctgagggagg ccgtcggcac ggcgtcgacc agccgagcgc
     5101 tcgtcgcgaa cccaccggtt gtcgtgccaa gcgagcatga tctcgacgag ctggcggcgt
     5161 tgctcaacgc gagcgagaag acgacgctct tctgcggccg cggctgcgcc ggggcgcatc
     5221 aggcgctgct gcagctcgcc gatgcgctga aatcgccgat cgtccatgcc tttggcggca
     5281 aggaatatgt tgaatacgac aacccgttcg acgtcggcat gaccgggctc atcggcttca
     5341 gttccggcta tcatgcgatg ctggattgcg acacgcttct catgctcggc acggactttc
     5401 cctatcgcca attctacccg accgatgcca aaatcgcgca gatcgacata cggccggaaa
     5461 atctcggacg gcgaacccgg atcgatctcg ggctcatcgg cgatgtcaac gcaacgatcg
     5521 cggcactctt gcccaagctt aaggtcaaag aaggccgctt tcatctcgat gaaagccttg
     5581 cccactaccg caaggcacgc gagggcctcg atgacctcgc cgtcgcgggg ccaggcgaca
     5641 aacaaatcca tccgcaacgg ctcgccaagc ttgtcagtga atatgccagc gatgacgcgg
     5701 tcttcacctt cgatgtcggc acaccggtcg tctgggcggc gcgctatttg aaaatgaacg
     5761 ggcggcgccg gctgatcggc tcgctaaacc acggctcgat ggccaacgcc atgctgcagg
     5821 cgatcggcgc gcaagtggcc ttccctagcc ggcagatcat cagcctttcg ggcgacggcg
     5881 gtttctcgat gatgatgggc gactttctga cgctcgtgca gcataagctg ccggtcaaga
     5941 tcatcgtctt ccagaatcag acgctgggtt tcgtcgcgct ggagatgaag gcggcgggat
     6001 tcctcgaaac cggtgtctca ctcgcaggcg tcgatttcgc cgcgatggcc cgcgccattg
     6061 gcgttcatgg tatccgggtc gatgacccgg cagacctcga agacggcgtg aaaaacgttc
     6121 tggcgcatga gggtccggcc ctgctcgatg tcaggaccgc gacgcaagaa ttggccatgc
     6181 cgccggcgat caagctagac caggtgaagg gcttcggact ttgggctctg agggcggtga
     6241 tgaacgggcg tggcgacgag ttgattgaag tcgcgaggac ccatctgcgg cgttgaatca
     6301 cgcatggcga ccgccatgcc gttggcaacc gtcgccttat tagctctaaa ttcatcggcg
     6361 agcagtcagc catggctcgt ggcggatgcg tcactcgcaa catagggctt caccatcttc
     6421 ttcggatcga cgaggcgatc gaattcggcc tcggtgacaa agcccaactt caaagccccg
     6481 tccttcaaag tgagatcatt gtccatggcg taatgggcga tctttgacgc tttgtcgtag
     6541 ccaataaccg gcgacagcgc ggtcacgagc atgagggaac gttcgacgta ttcattgatt
     6601 ttcttgagat tgggcttcgt gccttcgatc agaaactttc taaaattcac gcagccatcc
     6661 gtcaggatcg taatagagtg cgcgacgtta aagatcatca gcggcttgta gacgttcatt
     6721 tcgaggtaac cgcctgcgcc gccaaagccc accgcgacgt cagcagccat cacctgaaca
     6781 gcgatcatcg tcagggcttc gcattgggtc ggatttacct tgcccggcat gatcgaggag
     6841 ccgggttcgt tctcgggaat ctccaattca gcaaagccag cgcgtgggcc gcatgacatc
     6901 agacgaatat cgttggcaat cttgtagagc gacacagcga gcgtgcgcaa agttcccgac
     6961 agatgaacca gcgcatcatg tgcgccctgc actgtaaatt tgttcggcgc ggtgatgaaa
     7021 ggtaggccgg tgagccgagc aatttcggca gccgccgctt cggcgaaccc cggcgccgaa
     7081 ttaatgccgg ttcccactgc tgtgccgccg agcgccaagc ggtacacgcc ttgcacagct
     7141 gcctcgatgc gctccagatc ctccgacaac atccctgcgt aaccggacca ttcctggccc
     7201 aaagtcagcg gcgtggcatc ctgcatatgc gtgcgcccaa tcttgacgat gtcgctccac
     7261 gcttcggatt tcacctcgat cgcgtcgcgc aacgccgcga ccgcgggaat aaggcgctgc
     7321 ttaacgccga cggccgccgc gatatacata gcggatggaa aggcgtcatt cgatgactgg
     7381 gccatgttga cgtgatcgtt ggggtgcacc ggcgcatggc taccgagcgc ggtcccggca
     7441 agctgactgc agcggttaga gatcacttca ttgacattca tgttgaactg ggtgccgctg
     7501 cccgtcatcc agacatgcaa cggaaacata tcgtggtgtt ggcccgccag aatttcgtcg
     7561 caggtctgaa caattaattt gtgccgccga tcgtcaaggc ggtcgccagc atggtttgcg
     7621 ccggcggcgg ctttcttcag aatcgcatag gcagtgatca tttcgcgcgg aattaaatcc
     7681 tttccgatac tgaaatgctc aagcgagcgc tgagtttgcg caccccacaa tttgtctgca
     7741 ggcacttcga caacgccgag gctgtcggtc tctcttctca cgtcgaccat gggacgctcc
     7801 taggttctta aacggtttca agccctagaa ctggtgcggt gtccgcgcgc gtaagacggc
     7861 acgcctcggc gtcgatcttg agatcgatac cataggttgg gatcatccgt tttagtgctg
     7921 gcaaccagcc atcatctgtc agtttatcgg caaaacactt ctcaagtacc tcctgagcga
     7981 tgaaggccgc ggtcgaagcg ccaggcgagg ccccgagcag ggctacgagc gatttgtcgc
     8041 tggctgctac caactcggtt ccgaattcga gcacgccgcc tctttcttta tctggcttga
     8101 taatctgcac gcgctgatcg gcgaccgctt tgtaccaatc gcgccgagca gctttaggga
     8161 aaaactgctg aaggagcgca aattgatgac tggaactttg gaggacctgt ccaatgagat
     8221 attcgctcag agcaaagtta tcgcgcgcaa catcgagaag cgggcgaatg ttgtccggcc
     8281 taatcgactc gaataaatct gtcagtgatc cgtacttgag gaacttgctg gaaaagccag
     8341 cataagggcc gaaaagcaaa tgcttccggc cttcaatgac acgtgtgtcg aggtgaggga
     8401 ccgacatggg cggcgaaccg gtcgccgcct tgccgtagac tttggcatga tggcgttcgc
     8461 tcacggcgtc gacgtcgcag cggagccaga tgccactcac cgggaagccg ccataacccc
     8521 ttccttcggg aatttccgat ttctgcagaa gctccagtgc ggctccgcct gcgccgatga
     8581 agacgaactt cgccgacgcg gtctggcggg tgccgtcata ggtgtcctcg accgttactc
     8641 gccaacggcc atcgcctccg cgcttgagat cgacaacttg gcgattatag cggaccgcaa
     8701 accccggctc tgcagaaagt tgagctacga ggagatgggt caaagcacca taatcgacgt
     8761 ccgcgccagt caccatccgc gtggctgcaa tttgctgatc ttccgggcgc ccgtcgataa
     8821 tgagaggtgc ccaacccgcg atcgtggtcc ggtcctcact atattccatg ccctcatagc
     8881 aatgatgggc agacatttcc ttgtagcggg cgcgcaggaa ggcgacattc tcccgccccc
     8941 aaacaaaact catatgtggg catgcatgga tgaaggcgtg cggatctgga atggcgccct
     9001 ttctgacgag atgagtccag agttggcgcg aaatgtcgaa ttccgtgttg acttctaacg
     9061 ccttggaaat atcgacgctg ccgtcagaac gctgcggcgt gtaattcaac tcgcaatttg
     9121 cggcatgccc ggtgcctgcg ttgttccaag cttgcgaact ctcttgccca caatcgtcga
     9181 gcctctcgaa catcattatc gtcagcgaag gctcgagctc tttgagcacg gtggcaagcg
     9241 ttgcgctcat gataccggct ccgatgaaca cggcatctgg attgtcttcg ttcacaatcg
     9301 acatagctca cctcggtttg attacggttt cggcagagcc tggaacggca acgggacgcc
     9361 ggggcggtaa acgacgtaaa cgcgccgcca aggctcttca ccgatcaact tccaccgatg
     9421 gccggatcct gtattgtcct gtgcgagcag aatatcgcca ggtcgaatga caaaggtctc
     9481 gccgccgcgc gtctcgaact cgaggacgcc gctcaatgta atgacaaacc gtggcaccgg
     9541 gtctttgtgc caagcgtagg aaccaccgga tttggtctct tgaaaagata cgttaacggc
     9601 gtctaccgct tcggtaagac tatcattccg ctcccctgat gaaagggcga tgaggccttc
     9661 ctcgaagtgg gaatctccgt catctcccgt ccacatgcgg acacatctga tcatgggcct
     9721 tcctccttca gatccatgta cgaatatcag cgttgttgtt ggccgcccga tataccttcc
     9781 ccgagccagc cgctaaattg cagcccagct gggcgcgggc atgaaggtcg tagtcactgc
     9841 catccgacag tgccgttccg caggctgcat ttcaaatcga gttaggacaa gagatgtaag
     9901 tggcggaggg agcgggattc gaacccgcga taggctttca cctatacaca ctttccaggc
     9961 gtgcgccttc aaccactcgg ccacccctcc tctcgtctcg cccgcggcgg gtggcttacc
    10021 cccaatcgat cacgattggc gcgcactata atcagcgaag acgccagcgc aagcggcctc
    10081 ctaaaccttc ttgggcaagc gatccttgga ctgcataatt ccgacgaatt atacaattac
    10141 gcatgtggac gaggtgcggt gggcgtggca aatgaagcca ccgcttcgga ttcgccgatg
    10201 tttcgcttct tccttcgtct gatcggcctt ttgctgctcg ccggcgcctt tgcggcgctt
    10261 atcgtcgacg gcacccgcag catcgccgcg ggcgcgctcg ctctgacccc tctggcgcag
    10321 acgctcgcct ggctctcgcc gcagaaattc gaagcgctga aaccccaatt gcagcatcat
    10381 gtgccgcatt tcgtctggga cccgctgatc gtccatcttt tgaccgcgcc gacctggatt
    10441 gtcgccgggg cgcttggcgc gctggtcctg cttattacgc aaaagcggcg gccgaaaatc
    10501 ggctattcga accgcgaata ggcgcgcaac acgcccgatc ggcactagcg tctcggccta
    10561 atcctcgaaa gcgttttcgc gcagggcatt caactcctgc gcgaagcctt gcagccgttt
    10621 ttccaggaac tgctgtaaac gccgggcgct gcgcggcgat tcggaaagcg cccgctgaaa
    10681 cagcgtccgt gaaatctgca ggacgccgga cggttcccgg gcgatggcgg tcgccgggcg
    10741 gcgtgtgggg gcgatcaatg ccatctcgcc gatcagggcc ggtggctgga tgactttctc
    10801 ctcgcgcgac cgatcgccca cctcgaggac gacgcaaccc gagcgtagga aatagccgcc
    10861 gtccgagggc tcatcgcggg aaaacagaac ctcaccgctg cgcagaatgc gttgccccgc
    10921 ggaaagcgca atgaagcgca aggcttcggg ctcgagcacc gcaaagatcg ggtggcgcgt
    10981 gaggtcgtgg agatcgtcgt cgcgagccat gctctactcc cgtcagggca taagcttgta
    11041 accgccggcc tcggtaatca aaagctgcga tttcgccgga tcctgctcga tcttctgccg
    11101 cagccggtag atatgcgtct caagcgtatg ggtcgtgacg tttgcattat agccccagac
    11161 ctctttcagc agcacgtcgc gggtgacgac agattggcgc gcccgataga gaaagcgcag
    11221 gatcgacgtc tctttctcgg taaggcgcaa tttactgccc ttctcgctga tgagatgttt
    11281 ggaacccggc tgaaaggtaa acggtccaat gcgaaacagg gcgtcgtcgc tcgcctcatg
    11341 ctagcgcaga tgaacgcgca tacgcgccag cagaaccgaa aaccggaacg gattggtcac
    11401 ataatcgttg gcgcccgcgt cgagcccgtc gatcgtgtcg gcatccgaat catgaccggt
    11461 gagcataatg atcgggtttt tatagccctc accgcgcaat tgccgcacgg cctcgcgtcc
    11521 atccatgtcc ggcaggccga catccatgat aatgagatcc ggcgccgcct cgcgcgctgc
    11581 caaaatcgcc gtctgtgcgg tggccgcatg cagcggctcg aactcggcgt gcaaagcaag
    11641 ttgttcgacg agttcgtcac gaagatcgtc gtcgtcatcc gcaatcagaa tcttgcgaac
    11701 ctgcgtcatg gctggtccta tgtctcaggg ccgcgtcggc ggcgtttaaa agtcaatctc
    11761 agtgacaaac ccacggaaac agcgcggccc cggcctgtcg aaagcgcgca aaggatgcgg
    11821 caagccgaag gttgagacgc gtttgcgccg gattgttgcg acggcgttac cgcgctttac
    11881 tcccgacgcg ccggcgcggg gacgcctgca cgcgggccag accgtgttca cctgcgcgct
    11941 cggagcgact gcaatcagcc accgtaagcg cgaaggcgac cgtacatcgc ctgggggtca
    12001 ttttcggctg attgaaggct ttttcaagcc gttaatcggc cgccgcccgc gcacctcgct
    12061 gccaatttgt gccattagcc gcgagcttgg ctggtgcgac gacccggact ccggcaatta
    12121 taaccggctt attcgcctgc cagcccaggc gcgccacgaa acgctttggc gcgacgacgg
    12181 tctctacgat ctcgtcatcg ttctcgacta taatctgtca ccccggcgca agggcgcggg
    12241 cagcgcgatt tttcttcatt gcgcgcgccc ggggtttaca ccgaccgaag gctgcatcgc
    12301 gctacggcaa gaggatctgc gcaagctttt gccacgcctc gcacgtcacg caatcttgat
    12361 cgttcaacgc taagttttcg ggagcgaaac acagtgaata tcctcctctg cggccgtctt
    12421 gcgatgctca ccctgtccgg cctgacgctc tcggctgccg gggtgtcagc ggcccccgca
    12481 ccgcaaacct attgcgccaa ggtcgttaac gacgacgagt tgcggggggc gccacattcg
    12541 ctctcgccgg cgatcaaaca gattttccat atcggcggcg cttatgcggc gcagacgacg
    12601 cgctaccgct gcg
//
LOCUS       DASWOG010000597        10944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_289164,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000597 DASWOG010000000
VERSION     DASWOG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289164"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1432
                     /locus_tag="VGB93_12215"
     CDS             290..1432
                     /locus_tag="VGB93_12215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0146881.1"
                     /translation="MAMITNIQDFRHAARRRVPRAIFDYADRGSYDEITIQANRADLN
                     AIRLRQRVMIDVSRRSLATTLVGEPTALPLAIAPTGLTGLFHRDGEIVGARAANAAGI
                     PFCLSTMSICSIEDVREAVDNPFWFQLYVMKDRGFAASLIERAKIASCSALVLTLDLQ
                     IQGQRNRDIKNGLSVPPRLTLANAVDIALRPEWLFGVLMGKRRSFGNLEGRIEGADSF
                     TTLSQWIAGQFDPSLNWTDLQWIRSLWPGKLILKGILDVEDARIAARCGADAIVVSNH
                     GGRQLDGAASSISMLSRIVDAVEGRVEVLFDGGIQSGQDVLKALALGARGCLIGKAFL
                     YALAAEGEAGVARLVKIVAHELDVSMALCGLADVTKADRRILANDV"
     gene            complement(2340..3662)
                     /locus_tag="VGB93_12220"
     CDS             complement(2340..3662)
                     /locus_tag="VGB93_12220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146882.1"
                     /translation="MSVSADQVFSKVLKRLVPFIMLLFFISLLDRANISYAALQMNKD
                     LGLGPSAYGFAAGIFFVGYFLFEIPSNIALAKIGARKWLGRIMITWGLVAIAMAWVSG
                     AASLYSLRFLLGVAEAGLLPGVMFYLARWIPGRARGATLSVLMATTAIAYVIGAPLST
                     WLMSFNGVLGLKGWQLMFVVEGIPAVIFGLVTLATLPETPSEAKWLTGPERDTLNNAI
                     ARESDAKQAHGPSSMWQGLTDRRILLASVFTFFCICVNFGTVFWLPQIIKSFGHISNM
                     EVGLLSAIPYALGGIGMIISGRHSDQTGERRWHLFSGAALAAVGYTAAAFAPEPVYAF
                     IAICIAAIGIWSTFGLIWAYAGDLVAGPAAATGFAVINSIGSAGGFVGPFLIGWVRAH
                     THGFSGGLLTLAAFGAFTALFALMLKGRPIPTSTETLVASADPMVTVE"
     gene            complement(3940..4692)
                     /locus_tag="VGB93_12225"
     CDS             complement(3940..4692)
                     /locus_tag="VGB93_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0146883.1"
                     /translation="MIKMLNGKTVVVTGAARGLGRDIAIACGRHGAALILGDILEEQG
                     LETVACLKADGVTARFVPVDLGDPESVASFGHNIAANERTVHGLVNNAAIATNVGGAK
                     FEDIDIELWDRVMRVNVRGTWLMTRALSPLLPEGARIVNMASDTALWGPPRLLAYVTS
                     KGAVISMTRSMAREFGPRGIGVVALAPGIMKNEATDYVPAERHREYETRRAVPGPQFP
                     EDIVDIVAFMLTPHALPLTGQVIATDAGFTFN"
     gene            complement(4698..5741)
                     /locus_tag="VGB93_12230"
     CDS             complement(4698..5741)
                     /locus_tag="VGB93_12230"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0146884.1"
                     /translation="MAEPDFKQRVEERLNQGLLGQWYVVAKSADVPFGKTLPIKVLGR
                     RLVLWRGSDGVHCLEDYCPHRGARLSRGEVLGTQIACRYHGVSLDGDGRIIQVPAMPE
                     CSLEGRKAAASFVVREACDAIFAYFPSLEKPEPDELELPYELESDDYERFLVTAPWAC
                     NYRYALDNIADPMHGCYLHADTFTLAYGAKQDLMQIEETEDGFIIGRVGQRGANFDWT
                     EVVTDKTAFYCRLDIPYPPAGGPGGPMRILTFLTPVDENNCRIFFWRTRKVSGVAREA
                     WRFLYRAKFEPRHWYVLEQDREMLSAMPADARKREMLYQHDIGVSRLRKILLSRAKAQ
                     IEHEDRAALQAAG"
     gene            complement(5797..6090)
                     /locus_tag="VGB93_12235"
     CDS             complement(5797..6090)
                     /locus_tag="VGB93_12235"
                     /inference="COORDINATES: protein motif:HMM:NF042599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEY0146885.1"
                     /translation="MTSSAPQDVHARLAKQALARPLTPLEEALADTLEATFKTTGHDM
                     DEAVRVLSAKGVARPSGGAGPWSLDVLQTELAAINASLDAAYLARGPVELDQS"
     gene            6185..6805
                     /locus_tag="VGB93_12240"
     CDS             6185..6805
                     /locus_tag="VGB93_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0146886.1"
                     /translation="MMEDLIAALLTPTARGEVRDLAGAVFKEPVDLSGRQLGNVDLSR
                     AIFDAPVLARGAVFTGLAWLRGTTFKSSVDFSSAIFCSDARFDSAVFAGEANLSCVEY
                     RGIAAFDYTVFADAAFLDRMVCLANLSFDGATFDSALTLQDTECLGGFWANGTAFGRR
                     TDVTGLEIYGRVWMRGAKLSSGEAIPQSLMAAMRRSGAAYFVDNAA"
     gene            6907..8217
                     /locus_tag="VGB93_12245"
     CDS             6907..8217
                     /locus_tag="VGB93_12245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0146887.1"
                     /translation="MGPESRPRVPHIAIVGAGPSGLFAAQNLRRLVADAAIDVFDRLP
                     VPFGLIRYGVAADHQGVKAVARQFERLFEREGVRFFGNVHIGRDISVEELTEFYDVAL
                     LAAGLAGDRRLGIEGESLPGVLRAGPLTRWLNSHPDEAAFRPKLGARVVIIGNGNVAL
                     DIARLLLKTPQELSGSDLSSDRIAYLQNEGICDVEIVGRGGPGSARFDPAMLKQLASL
                     GAIQISVDWPPQGQAAFAQEDSRSAACIAALQAIDGHGCGTAPRRLRFRFNLVPTAIE
                     GTDHVTGVRFNAGGDPQRIRADSVVGAIGFAPDENVPVDLPRGAGDEVESCGWPRVFT
                     AGWFGRGPRGAIAENRLDARAVAESIAAQIVRAGILPGGRNGGVGALALLAERRVRHV
                     DYAGWLRIRDAEESARTADLVRLKINDWTSMMALADAPHLLDAE"
     gene            8214..8663
                     /locus_tag="VGB93_12250"
     CDS             8214..8663
                     /locus_tag="VGB93_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY0146888.1"
                     /translation="MMKISILFATETGNAETLADDLRDELASRHDVSTASLTNFSTAV
                     GNADLSLLLISTYGDGEVPAMAKCFVAALDAGDVPVAGRRFAIFGLGDKNYGPTFGRA
                     SRLVEERLIAKGALITGEREVHDASGPEFMDDQAKRWCARVLQALAL"
     gene            complement(8620..9480)
                     /locus_tag="VGB93_12255"
     CDS             complement(8620..9480)
                     /locus_tag="VGB93_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0146889.1"
                     /translation="MPHKDANVDGYIDGSAFWGGSATPSVAEQLVRKLRIAVLRGDLR
                     PGQKLKEIELCEQYDVSRATLREGFRILESERLFELIPNRGAFVARLGIKEIEEIHDI
                     WAMLTGEAVYRFAERCNAQDLKELETCVGKLRRGIEAGQPLAQMEATNRFFNFIHSRS
                     QNRMRHEMIIGLVSRLMFLRAQSLLNQGWSLLYAEEIEAIVAAARNKSPDDARFAVKR
                     HIASVCAAAKQIILVAERKKPEAEDATEVATKPGKTVQMAPRDKAPARKPQTQRAKGR
                     APAARARTSA"
     gene            9683..10696
                     /locus_tag="VGB93_12260"
     CDS             9683..10696
                     /locus_tag="VGB93_12260"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY0146890.1"
                     /translation="MSYSRSEAKAWAKKNWRGACNVIMPSFTSDLKTLNEAGIRHDVR
                     RNIELGFWGALLVSECGTTFEEYRRFMEIAIDEAKGRHHLLVHGTFDTREEIIEAAKA
                     GEELGASGLLLGHPNSFYPETEDELYAHIAKVAAHTDLAVSLFVTSQMNLGRLHASGY
                     PPNVLVRAADIENVVAVKYEVGRPGIAGDLEVWKLLRDKNILFSDPLEAHSPLTVEMF
                     GMQWMGTSNYEYWGSAVPQYFDLLLKGEYEKAMEIYWRINPARQARVAVQATVVGGNF
                     IHRYLWKYQGWLQGYNGGRIRQPAMKLTDAQMRQVRDGLVKSGFDIADESPADFYRSR
                     NPD"
     gene            10703..>10944
                     /locus_tag="VGB93_12265"
     CDS             10703..>10944
                     /locus_tag="VGB93_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146891.1"
                     /translation="MTLLLGHEDVIASVTMTEAISAMEAAFREEADGTMILPPRTNMS
                     VGKGWLRVGPVAMPTSGWMGFKAMNLVPGIGLRYQV"
BASE COUNT         2205 a         3104 c         3328 g         2307 t
ORIGIN      
        1 cctcctccca catctgcggc gtggcgatta tctcagacaa cgatggtccg ggcgccagtt
       61 tttagccata cagaatagtg tcgacattaa tgcttttcat actcgtgtca acggcttaat
      121 aaaccgggaa cggccctaaa tatgtcggca ctttggcgcg agccgtttgg gtcgatcgct
      181 tcagcccaaa cgagcgtcac atgtgtccga taccattaca gtagttctgt cggcagcgtg
      241 tttaggcgat cgagcaggtc gcgcacaagc aacaagcatg taatcgcgga tagccatgat
      301 aacgaacata caagatttta gacatgcagc ccggcggcgg gtgccccggg caattttcga
      361 ctacgccgac cgcggctcct atgatgagat cacgattcaa gcgaaccgcg cagacttgaa
      421 tgcgatacgg cttcgtcaac gcgtgatgat tgatgtatca cgacgttcct tggcgacaac
      481 cttggttgga gagccgacag cgctaccact ggcgatcgcg ccaaccggat tgaccggatt
      541 atttcatcgg gacggcgaaa ttgtcggcgc gcgcgccgcc aacgcggcgg gaattccgtt
      601 ttgtctgagc accatgtcga tctgttcgat cgaggacgtc cgcgaggctg tcgacaatcc
      661 gttttggttt cagctttatg ttatgaaaga cagaggcttt gcggcatcgt tgattgaacg
      721 cgctaagatc gccagttgtt cagcgctggt attgactctc gacctgcaaa ttcaaggaca
      781 gcggaatcgc gacatcaaga atggattgag cgtgccgccc cgtctgacgc tggcaaatgc
      841 agtggatatc gcgctgcggc cggaatggct tttcggcgtc ttaatgggaa agcgccgatc
      901 ttttggtaat ctggagggcc ggatcgaagg cgcggacagc tttacaacgc tgtcgcaatg
      961 gatcgctggt cagtttgacc catcgctaaa ttggacggat cttcaatgga ttcggagtct
     1021 ttggccggga aaactgattt taaagggcat tcttgatgtc gaggatgcgc gcatcgcagc
     1081 gcgctgtgga gctgatgcga tcgtcgtctc aaatcacggt gggcggcagc tcgatggcgc
     1141 cgcttcgtca atctcgatgc tctcgcggat tgtcgatgcc gtcgaaggcc gcgtcgaagt
     1201 cctattcgac ggcggaatcc aatccgggca agatgttttg aaagcgctcg cgcttggtgc
     1261 cagaggctgt ctgataggaa aagccttcct ttatgccttg gccgctgagg gagaggccgg
     1321 cgtcgcgcgg cttgtcaaaa tcgtcgcgca tgagctcgat gtcagcatgg cgctttgcgg
     1381 ccttgcggat gtcacgaagg cggaccgccg cattttggct aacgatgtat agctggcgca
     1441 aactaaacga tcctcttttc cgacattcgg ggcggggaaa tccccgcccc gcgtcaattg
     1501 ttcgaacagc cctcggccat ggtatgtggg agaacccgtt gctgggacga ggaagagcag
     1561 ggcaccctcg tggaagcaag cggcgattac ccggaaccac taggtgttta aaattagata
     1621 gcaatttcaa ttatataggt gttcgctgtt ttgttctgag tggcgcgcca atcctttcaa
     1681 tgacttagtt tattttgcac tgcggccggt ccaatatctg gatatgcgca tcgcacatag
     1741 ccatttttgg agtttccgaa gtgcctttag cgatcctggt tcctccgcct aatacagcgt
     1801 gctttcgctt cgtgacgacc caatgaagtt tgatgtccgg ctttatctgg tttcgagaaa
     1861 ccgagggaga tgtgagccag ccgaggccgt gacggccgac ggcacgatca ttggcgcata
     1921 ttcccctccg tcagccgaat gacttcgaca aaacatcgac attgcagata ttcgtcgatt
     1981 tgccgatgac accggaaatg tacaggcatc ggcgggaccg tttgcaatac cgcgtcgatc
     2041 aacggtgaca tacgatagga aggcgatcct gagctttatg cttttcgacg gcaaattaca
     2101 tgacgcacat aaagttatct gctctgcgtc gcagatcgat atgctaggct gggggcacag
     2161 cgaatggcgg aagcgaccgt tcgagctgaa ataccattca atcgatccgg cgcgtgatac
     2221 cacccggccg ggcgaaaggt tgcatgccga gatggcacgt ctgaaatttg atgctgacgt
     2281 cgccaggcgt cacgggctcg cacttgaaag cgctggcgtc gaggacatca cggacttcat
     2341 tactctacgg tcaccattgg atcagcgctc gcaaccagcg tttctgtcga tgtcggaatc
     2401 ggacgtccct taagcatcaa agcgaaaagg gccgtgaagg cgccgaaggc cgcgagcgtc
     2461 agcagacctc ccgaaaaccc atgcgtatgc gcgcgcaccc agccgatcaa gaagggcccg
     2521 acgaagccgc ctgcgctacc aatgctattg atgacggcaa atccggttgc cgcggcagga
     2581 ccggcgacga gatctcctgc ataggcccag attaggccga acgtgctcca gatgccgatc
     2641 gctgcgatac agatcgcaat aaaggcatag accggttcgg gcgcaaacgc tgctgccgta
     2701 tagcccacgg cggccaacgc tgcgccggaa aataggtgcc agcgacgctc acccgtttgg
     2761 tcggaatgcc gccctgagat gatcattccg atgccgccaa gcgcgtaagg aattgcggag
     2821 agaagcccga cctccatatt cgaaatatgg ccaaagctct tgatgatttg cggtagccag
     2881 aagaccgttc cgaagttgac gcaaatgcag aagaaagtga aaaccgacgc aagtagaatg
     2941 cggcgatcgg ttaaaccttg ccacatggag gagggaccgt gcgcctgttt cgcgtcgctt
     3001 tcccgcgcga tagcgttgtt caaagtgtct cgctcagggc cggtcagcca tttcgcttcg
     3061 gagggtgtct ctggcagcgt cgcaagcgtc acgagaccga agatgacggc agggatacct
     3121 tcgacgacga acatcaattg ccatcccttc agccccagca cgccgttgaa cgacatcagc
     3181 caggtcgaga gcggcgcgcc gatgacatag gcgattgctg tcgtcgccat gagcaccgag
     3241 agcgtggcgc cccgcgcgcg gccgggaatc cagcgggcaa gatagaacat cacgcccggc
     3301 aacaagccgg cttcagcgac gccgagcagg aagcgcagcg aataaagcga agccgcgcct
     3361 gaaacccagg ccatggcgat ggcgacgagg ccccaggtga tcatgatgcg gccaagccat
     3421 ttccgagctc cgattttggc gagcgcgata ttgctcggga tctcgaagag gaaatagccg
     3481 acgaagaaaa tacccgcggc aaagccgtag gcgctgggcc cgagcccgag atccttgttc
     3541 atctgcaaag ccgcgtagct gatattcgcg cggtcgagca aactgatgaa gaaaagcagc
     3601 atgatgaagg ggacgagccg cttcagcact ttcgaaaaca cttgatcagc cgaaaccgac
     3661 atttgatatc ccccttcgtc accttggcat tctcattttg tcagacaatg caatcgcagt
     3721 gccgttctgg ccaagtatct gggcgaagag tgcaatcgga tgagcggcgc gtatctgctt
     3781 cgtataactg gaaaagtcgc gcccaaagcc tgtgcttttc gcctaattgt cagacaatat
     3841 gactttggtt ttttatgcca gcaacaggaa ttatttgcgg ggcaccggaa atcggcgagg
     3901 tcccgctgac gttagggtat gagtccgaag ctacgcgggt cagttgaagg tgaagccggc
     3961 gtccgtagcg atgacttgcc ccgtcagcgg cagcgcatgt ggggtcagca tgaacgcaac
     4021 gatatcgacg atatcttcgg ggaactgcgg gccgggaacg gcgcgccgcg tttcatattc
     4081 ccggtgccgc tcggccggca catagtcggt tgcctcattt ttcatgatgc ccggcgcgag
     4141 cgccaccacg ccgatgccgc gcggcccgaa ttcacgcgcc atcgaccgcg tcatggaaat
     4201 caccgcgccc ttgctcgtaa cataagcaag cagccgtggc ggtccccaca aggccgtatc
     4261 gctcgccatg ttgacgatcc gcgcaccttc cggcagcagg ggcgacagcg cgcgcgtcat
     4321 cagccatgtg ccgcgcacgt tgacgcgcat gacgcgatcc caaagttcga tatcgatatc
     4381 ttcgaatttc gcgcccccga cattggtggc gatcgcggca ttgttcacga gtccatgcac
     4441 tgtgcgttca ttcgcagcga tgttgtggcc gaatgaagca acggactctg gatcgccgag
     4501 atcgacgggg acaaaccgtg ccgttacgcc gtctgccttt aggcacgcga cggtctccag
     4561 cccctgttct tcgagaatgt cgccgaggat cagcgccgcg ccgtggcggc cgcaagcaat
     4621 ggcgatgtcg cgcccgaggc cgcgcgcggc cccggtgacg acgacggtct ttccgttcag
     4681 catcttaatc attccaatca gccggcggct tgcagggcag cgcgatcctc gtgttcgatc
     4741 tgcgctttgg cgcgactgag gagaatcttg cgtaatcgcg agacgccgat atcgtgctga
     4801 tagagcattt cacgcttgcg cgcgtctgcg ggcatagcgg aaagcatctc gcggtcctgc
     4861 tcgagcacat accagtgccg cggttcgaat ttcgcccggt agaggaagcg ccatgcctca
     4921 cgcgcgacgc cagaaacctt ccgcgtgcgc cagaagaaga tacggcaatt gttttcgtcg
     4981 accggcgtca ggaacgtgag aatccgcatg gggccgccgg gaccgccagc cggcggatag
     5041 ggaatgtcga gccggcaata gaacgccgtc ttgtcagtca cgacttcggt ccaatcaaaa
     5101 ttggcgccac gctgaccgac gcggccaata atgaatccgt cttccgtctc ttcgatctgc
     5161 atcaaatcct gcttggcgcc gtaggccaaa gtgaacgtgt cggcatgcag gtagcagccg
     5221 tgcatcgggt cggcgatatt gtcgagcgcg tagcgataat tgcaggccca gggcgcagtc
     5281 acgaggaagc gttcgtagtc gtcgctttcc aattcatagg gcagttcgag ttcatccggt
     5341 tcgggcttct caaggctcgg gaaatacgcg aagatcgcgt cgcaagcttc gcgcacgaca
     5401 aagctcgcgg ccgccttgcg cccttcgagc gaacattccg gcatggccgg cacctgaatg
     5461 atgcggccgt cgccgtcgag cgagacgccg tggtagcggc aggcgatttg cgtgccgagg
     5521 acttcgccgc gcgagaggcg cgcgccacgg tgtgggcagt aatcttccag gcagtgaacg
     5581 ccatccgagc cgcgccacag aaccagacgc cggcctaata cctttatcgg cagcgtcttg
     5641 ccgaatggga catcggcact tttggcgacg acataccatt gtccgagcaa gccttgattg
     5701 aggcgctcct caacgcgttg tttgaaatcg ggctcagcca tgtccgtccc tttgcgtctt
     5761 caacttccaa atgcggcaag ccgaccctta tgttcatcag ctttggtcga gctcgactgg
     5821 gccgcgcgcg agataagccg cgtcaagcga tgcgttgatt gcggcgagtt cggtttgcag
     5881 tacatcgagc gaccaggggc cggcgccgcc ggagggccgc gccacgccct ttgcgctgag
     5941 cacgcgcacg gcctcgtcca tatcgtggcc agtggttttg aatgtcgcct caagcgtgtc
     6001 cgcgagcgcc tcttcgagag gcgtcagcgg ccgggcgaga gcttgtttcg ccagacgagc
     6061 atgcacgtcc tgcggcgcag aggatgtcat tttgaacgcc ccatattgtc agacaataat
     6121 gagcggcctt atacaaacct gtcaagcggg taaacgctgg cgtcaagctt caagggcgag
     6181 cgggatgatg gaagatttga tagcggcgtt gcttaccccg accgcgcggg gcgaggtgcg
     6241 agacctcgcg ggggcggtgt ttaaagaacc tgtcgatctg tccggccgcc aattgggaaa
     6301 cgtcgattta tcgcgcgcga tcttcgatgc gccggtcctc gcgcggggcg ccgtctttac
     6361 cggtcttgcg tggctgcgcg ggacgacatt caaaagttca gtggatttct cgtccgcaat
     6421 tttttgcagc gacgcgcgtt tcgactccgc ggtttttgcg ggcgaggcca atctttcatg
     6481 cgtggaatac aggggaattg cggctttcga ttacactgtt tttgccgacg ctgcctttct
     6541 cgacagaatg gtctgtctcg ccaatctctc ctttgacggc gccacattcg actctgcgtt
     6601 gacgttgcag gacactgaat gcctcggcgg attttgggcg aacggcacgg cgttcggccg
     6661 gcgcacagat gttacaggcc tcgaaattta cgggcgcgtg tggatgcgcg gcgcaaaatt
     6721 atcgtccggc gaggcgatac cccaaagcct catggcagcg atgcggcgaa gcggggccgc
     6781 atatttcgtc gacaatgcag cttgacgggc gtttgacatt gtctgacaaa atggcaactt
     6841 attgggtgaa tgccttagcc tttcgtttgg ggcaggctcg aagttctgac tgtaggtatc
     6901 gagataatgg ggcctgaaag ccgtcctcgc gtgccgcata tcgccattgt gggtgccggg
     6961 ccgtccggtc tgtttgccgc gcagaatttg cgccggcttg tggccgacgc ggctatcgat
     7021 gttttcgacc gcctgccggt tcctttcggg cttatcagat atggcgtggc ggcggatcat
     7081 cagggagtca aagctgttgc gcgtcaattc gagcggctat tcgagcgcga aggcgtccgc
     7141 ttctttggca atgttcatat cggacgcgac atctcagtcg aggaactgac ggagttctac
     7201 gatgttgcgt tgctggcagc aggtctcgcg ggcgaccgcc ggcttggcat cgaaggggaa
     7261 tcacttcccg gcgtgttgcg cgcggggccg ctcacccgct ggctcaacag ccatccggac
     7321 gaggcagctt tccgcccaaa gctcggcgcg cgcgttgtca tcattggcaa cggcaacgtc
     7381 gcactcgaca tcgcacggct attgctgaag acaccgcagg aattgtccgg ctcagatctg
     7441 agttccgacc gcatcgcata tctgcagaat gaaggtattt gcgatgtcga gatcgtgggc
     7501 cgaggagggc cggggagcgc gcggttcgat ccggcaatgc tcaagcaatt ggcgtcgctt
     7561 ggagcgatac aaatttccgt tgattggccg ccgcaagggc aggcggcatt tgcgcaggag
     7621 gattcccgtt ccgcagcatg tatcgctgct ctccaggcca ttgacggcca tgggtgcggg
     7681 actgcgccgc gcagactgcg tttccgcttc aatctcgtgc ccacggccat cgaaggcacc
     7741 gaccatgtaa cgggggttcg cttcaacgcc ggcggcgatc cgcagcgcat ccgcgccgat
     7801 tcagtcgtcg gcgccatcgg cttcgcaccg gatgaaaatg ttcctgtgga cttaccgcgc
     7861 ggggcggggg acgaagtcga gtcgtgcggc tggccacgcg ttttcacagc cggctggttt
     7921 ggccgcggtc cgcgcggcgc cattgcagaa aatcgcctcg atgctcgcgc cgtcgctgag
     7981 tcgatcgcag cccagatcgt gcgggcggga atcctgcctg gcggccgcaa cggcggggtc
     8041 ggcgcattgg ctttgctggc cgaaaggcgg gtgcggcatg tcgattacgc cgggtggttg
     8101 cggatccgcg atgcggagga aagcgcgcga accgccgatc ttgtccgcct gaagattaat
     8161 gactggacct ccatgatggc gttagccgac gcgccgcatc tgctcgacgc cgaatgatga
     8221 aaatctcgat tttattcgcc acagaaacag gaaacgccga gacgcttgcc gacgatctac
     8281 gcgatgaact cgcaagtcgc catgatgtct cgaccgcaag tcttaccaat tttagcaccg
     8341 cggtcggaaa cgccgatctt tcgctgcttt tgatttcgac ctatggggac ggcgaagtcc
     8401 ctgcgatggc aaagtgtttt gtcgcggcgc tcgacgctgg cgacgtcccg gttgcagggc
     8461 gacggttcgc tatattcggc ctcggcgata agaattacgg cccgaccttc ggtcgagcga
     8521 gccgtcttgt tgaagagcgg ttgattgcga aaggggcgct gatcactggc gaacgggagg
     8581 tgcatgatgc ctccggcccg gagttcatgg acgatcaggc taagcgctgg tgcgcgcgcg
     8641 tgctgcaggc gctcgccctt tagcgcgctg cgtttgcggc ttgcgcgctg gcgctttatc
     8701 gcgcggcgcc atttggacgg tttttcccgg cttcgtcgcg acctcggtcg cgtcttcggc
     8761 ctccggcttc ttgcgctccg cgacgaggat gatctgtttt gctgcggcgc agacggaggc
     8821 gatgtgccgc ttgaccgcga accgcgcgtc gtcggggctt ttgttgcgcg ccgccgcgac
     8881 gattgcctcg atctcctcgg cgtagaggag actccagccc tgattgagca gcgattgcgc
     8941 gcgcaggaac atcaggcgcg agaccagacc gatgatcatt tcatgccgca tccggttttg
     9001 acttcgtgag tgaatgaaat tgaaaaaccg attcgtcgct tccatctgcg ccagtggctg
     9061 tccggcttcg atgccgcgcc gtagcttgcc gacgcatgtc tcgagctctt tcaagtcctg
     9121 ggcgttacag cgttcggcga aacggtaaac ggcttcgccc gttagcatcg cccagatatc
     9181 gtgaatctcc tcgatttcct tgatgccaag ccgggcgaca aatgcgccgc ggtttggaat
     9241 cagttcgaaa agacgttcag attcgaggat gcggaagccc tcgcggagcg tcgcgcggct
     9301 tacatcatat tgttcgcaga gttcgatttc cttgagcttt tgaccgggac gcaagtcgcc
     9361 acgaagcacc gcaatgcgta gcttgcggac gagctgctcc gcgaccgaag gcgtcgccga
     9421 gccaccccaa aacgcagagc cgtcgatata cccgtccacg ttggcgtcct tatggggcat
     9481 gcgtctgcct ccattgtctc aaacgcagcg atggccgccg cgcgactcgt ttgagccttc
     9541 atcgcgatag cattccgtgt ccgccatgca agattttttg tcggacgata aacagccaaa
     9601 gcaggtcgac tcagggatcg ctttggttga cagacaaaaa ttgtcagaca ataatcgtct
     9661 gacaatgcgg cggagatttt ttatgagcta ttcgcgttcc gaggccaaag cgtgggcgaa
     9721 gaagaactgg cgtggcgcgt gtaatgtcat catgccgagc ttcacgtccg atcttaagac
     9781 gctgaacgag gccggcatcc gtcacgacgt gcggcgcaat attgagcttg gcttttgggg
     9841 cgcgctgctt gtctccgaat gcggtacgac cttcgaagaa tatcgccgct tcatggagat
     9901 cgcgatcgac gaggccaagg gccgccatca tcttctggtg catggcacgt tcgacacgcg
     9961 cgaggaaatc attgaggcag ccaaggctgg tgaggaactg ggcgcttcgg ggcttttgct
    10021 cggccatccg aattcatttt atcccgagac ggaagacgag ctttacgcgc atatcgccaa
    10081 agtcgctgca cacaccgatc tcgccgtctc gctgttcgtc acatcgcaga tgaacctcgg
    10141 ccggctgcac gcgagcggct atccaccgaa cgtcctcgtg cgcgctgctg atatcgagaa
    10201 cgtggttgcc gtgaaatacg aggtcggtcg gcccggcatc gcgggcgacc ttgaggtctg
    10261 gaagctactc cgcgacaaga acattctctt ctccgatccg ctcgaggcgc attcaccgct
    10321 aactgtcgaa atgtttggca tgcagtggat ggggaccagc aattatgaat attggggcag
    10381 cgcggtgccg caatatttcg accttctgtt gaagggcgaa tatgaaaagg cgatggaaat
    10441 ttattggcgg atcaacccgg cgcgccaggc gcgtgtcgcg gtgcaggcca cggtggttgg
    10501 cggcaatttc atccaccgct acctttggaa atatcagggc tggctgcaag gttataacgg
    10561 cggccgtatc cgccagccgg cgatgaagct gaccgacgca cagatgcgcc aggtacgcga
    10621 cggacttgtt aagtccggct tcgacatcgc cgatgaaagc cccgccgatt tttaccgcag
    10681 ccgcaacccg gattgacgtc ccgtgacttt gctccttggt catgaagacg ttattgcctc
    10741 cgtcacgatg acggaggcga tttccgcgat ggaagcggcc ttccgcgaag aagcggatgg
    10801 cacaatgatc ttgccgccgc ggacaaatat gagcgtcggt aagggctggc tgcgcgtcgg
    10861 tccggtcgcc atgccgacgt cgggctggat ggggttcaag gcaatgaacc tcgtgcctgg
    10921 catcgggctc cgctatcagg tcca
//
LOCUS       DASWOG010000598         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_179556,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000598 DASWOG010000000
VERSION     DASWOG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179556"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGB93_12270"
     CDS             <1..389
                     /locus_tag="VGB93_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0146892.1"
                     /translation="GLGDQLAHPNAHWRGFQGDDDLDGTLARAADGAPVLLLAHEPFI
                     FPRVPERVSLTLCGHTHGGQVNLPIITERFTEAEFGMKKVYGHIVEGGRHMIISAGLG
                     TSIAPIRLFRPPEIVLVNIGSESALA"
     gene            complement(458..1069)
                     /locus_tag="VGB93_12275"
     CDS             complement(458..1069)
                     /locus_tag="VGB93_12275"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0146893.1"
                     /translation="MKFRKIIWALPLFALLAGCEADGLATPDPSLSGRDQEFLALAPK
                     ADIPSQFHRYEVEDPTGQTPGTVVVDTKHMYLYYVMPHGKAMRYGIAAGSEAAGWTGT
                     AKIGAKQEWPHWMPPSDMLKRWPHLQPTADAGGLPGGPENPLGARAMYLYQGNKDTMY
                     RIHGTNEPEQIGQAVSSGCIRMSNIDAIDLYNRVKVGTTVVVE"
     gene            1290..2246
                     /locus_tag="VGB93_12280"
     CDS             1290..2246
                     /locus_tag="VGB93_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0146894.1"
                     /translation="MTTLLFSHPASLGHDTGPVHPESQARLGAISDALAAEPFRDLKR
                     EASPRVRLEPILRVHPPAYAAAIEAAIPEKGIAYLDADTPVSHGSWEAILHSVGGGIA
                     AVDAVMRGDADNAFVATRPPGHHASQTNAMGFCLFNNAAIAARHAQAVHGAERIAIVD
                     FDVHHGNGTQAIFWADPSVLYCSTHEMPLFPGTGAASETGAHGTIVNAPLAPGADGFV
                     FREAIESVVLSRLIAFTPDLVIISAGFDAHWRDPLASLNLTEADFAWVTTQLMEVAEH
                     CCDGRIVSLLEGGYDLVGLGRSTAAHVAALMGSDYASPSTGS"
     gene            complement(2219..3301)
                     /gene="ribB"
                     /locus_tag="VGB93_12285"
     CDS             complement(2219..3301)
                     /gene="ribB"
                     /locus_tag="VGB93_12285"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0146895.1"
                     /translation="MRHTVTEAIEAIARGEIVIVTDDDDRENEGDLICAASLCTPEKM
                     GFIIRHTSGIVCAPLPPEEARRLNLQPMVATNDAPLGTAFTVSVDVRHGLTTGISAEQ
                     RANTVRALANGNMGAADFVRPGHVFPLIAREGGVLMRSGHTEAAVDLCRLAGLPPVGV
                     ICELANDDGSVMAGAQIQAFADTHKLKRISVAQLIAYRQEREKLIERVKMFPVDGPSG
                     PLTGYAYATPYEPVHHFAFVQGEIGDGRNIPARLHRVDLIADVITGSAQIPKTLACFR
                     KEGRGVLVLLRDGTAGVPVLSDVKESASEAMRTKEWREIGVGAQILRDLGISSIRLRT
                     SSPLNYVGLSGFGIEIVAYEPVEGEA"
     gene            3362..4153
                     /locus_tag="VGB93_12290"
     CDS             3362..4153
                     /locus_tag="VGB93_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY0146896.1"
                     /translation="MLRKTGLDALLLRIALGAILLSSALTSARAQESGPCNAISEAGQ
                     RYTICTFDVRRARLRLFLAGTDGKPYSNFAAIAEALKSRGDSLTFAMNAGMFGTDYRP
                     VGLYVEGGRQLMAANTRAGSGNFHLKPNGIFYFDARHAGIMETSRYLQAHLHPEYATQ
                     SGPMLVIDGRIHPKIRPDGASAKIRNGVGVDDRGAVVFAISDAPVTFNTFARLFRDGL
                     GSGDALFLDGSISSLYAPELNRDDELMPIGPIVGVVKPEPLGRNP"
BASE COUNT          768 a         1350 c         1242 g          798 t
ORIGIN      
        1 ccggcctcgg cgatcaattg gcccacccga acgcgcactg gcgcggcttt cagggcgacg
       61 acgatctcga tggaactctg gcgcgagctg ctgacggtgc gcctgttctc ttgctggcgc
      121 atgagccctt cattttcccc cgcgtcccgg agcgcgtgtc actgactctt tgcggccata
      181 cgcatggcgg ccaggtcaat ctgccgatca tcacagagcg attcaccgag gccgaattcg
      241 gtatgaagaa ggtctatggc catatcgttg aaggcggccg ccatatgatc atctcagccg
      301 gactcggcac gtcgattgca ccgatccgcc tgttccggcc gccggaaatc gtgctagtca
      361 acatcggcag cgagagcgct ctcgcctaac gaaaacgcct gcatcaaaga tgcaggcgcc
      421 gaatcttctt gatgatgagc cgtgtattct gtccggttta ttcgacgacg accgtggtgc
      481 cgaccttgac ccgattgtac aggtcgatgg cgtcgatatt cgacatgcgg atgcaacccg
      541 aagacaccgc ttgaccgatc tgctccggct cgttggtacc gtggatacgg tacatcgtgt
      601 ccttgttgcc ctggtagaga tacatggcgc gcgcgccgag cggattttcc ggaccgcctg
      661 gaagaccacc ggcgtccgcc gtcggctgca gatgcggcca acgcttcaac atgtcggacg
      721 gcggcatcca atgcggccat tcttgcttgg cgccgatctt ggccgtgccg gtccagccgg
      781 cagcctcgga acccgccgca atgccgtagc gcatggcttt gccgtgcggc atcacgtagt
      841 agagatacat atgcttcgta tcgacgacga ccgtgcctgg agtctggccg gtcggatctt
      901 caacttcata gcgatggaat tgcgaaggaa tgtcggcctt cggtgcgagg gcgagaaatt
      961 cctgatcgcg ccccgaaagg ctcggatcgg gggtcgccaa cccgtcggct tcacagccgg
     1021 ccaacaaagc gaaaagtggc agtgcccaaa tgattttgcg aaatttcatt tcaacgatcc
     1081 ccgcctttat gaaggaaata acgcatcggc ggctgtctcg ccactgctac aagcgttcgt
     1141 agcctctggc gcagcaagaa gaggaaatgc tgtggcgcaa atgaaacatt ttaagggcca
     1201 gaatccgagc cgacggacgg cgcgttgacc ggcatgtcgc gcgccaatgc tacgctccag
     1261 gcgaatcact cccattgcat cggctgcccg tgacgacttt gctcttcagc catcccgcga
     1321 gtcttggcca cgacaccggc ccagtccacc ccgaatccca ggcgcgactt ggcgccatca
     1381 gcgacgcgct cgcagccgag ccatttcgcg atctcaaacg cgaagcgtcg ccacgggtca
     1441 ggctagaacc gatcctgcgt gtgcatccac cggcctatgc cgcggcgatc gaagccgcca
     1501 ttcccgaaaa aggcatcgcc tatctcgatg cagatacgcc agtcagccac ggctcctggg
     1561 aggcgatcct acattcagtc ggcggcggca tcgcagccgt cgatgccgtc atgcgtgggg
     1621 acgccgataa cgctttcgtg gcgacaaggc cgcccggtca tcatgccagc cagaccaatg
     1681 cgatgggctt ttgccttttc aacaatgccg cgatcgccgc acggcatgcc caagctgtgc
     1741 atggcgccga gcgtattgcc atcgtcgatt tcgacgtcca tcacggcaat ggcacgcagg
     1801 cgatcttctg ggccgaccca agcgtgctct attgttcaac gcatgaaatg ccgttgtttc
     1861 ccggcactgg cgccgcctcg gaaaccggcg cgcacggaac gatcgtcaat gcgccgcttg
     1921 cacccggcgc cgacggcttc gtctttcgcg aggcgatcga atccgtcgtt ctgtcgcggc
     1981 tcatcgcctt cacgccggat ctggtgatca tttcggcggg cttcgacgcg cattggcgcg
     2041 accctctggc cagcctcaat ctcacggagg ctgatttcgc ctgggttacg acgcagctca
     2101 tggaagtcgc agaacattgc tgcgacggcc gtatcgtgtc gctgctcgaa ggcggctacg
     2161 atcttgtcgg tctcggccgg tccaccgccg cgcatgtcgc ggcgctaatg ggctctgact
     2221 atgcttcgcc ttccaccggc tcataagcca cgatctcgat cccgaagccc gacaggccaa
     2281 cgtagttcag cggcgacgag gtgcgtagcc ggatcgatga gatgccgagg tcgcgcagga
     2341 tttgcgcgcc gacgccgatt tcccgccatt ccttggtccg catcgcctcc gaggcgcttt
     2401 ccttcacatc ggacaagacc gggacacccg ccgtgccatc gcgtagcaag acgagcacgc
     2461 cacgtccctc tttgcggaag cacgccaacg tcttcggaat ctgcgcgctg ccggtgatga
     2521 catcggcgat gaggtcaacc cggtgcaggc gcgccgggat attgcgaccg tcgccgattt
     2581 cgccctggac gaacgcaaag tggtgcacgg gctcgtaagg cgtcgcatag gcatacccgg
     2641 tcaacgggcc gctcggccca tcgaccggaa acatcttgac gcgctcgatg agcttttcgc
     2701 gctcctgccg ataggcgatg agctgggcga ccgaaatgcg cttgagcttg tgcgtgtcgg
     2761 caaacgcctg gatttgcgcg cccgccatca cgctgccgtc atcgttggca agttcgcaga
     2821 tgacaccgac cggcggcagg cccgcaaggc ggcagagatc gacagccgcc tcggtatgcc
     2881 ccgagcgcat caggacaccg ccctcgcgcg cgatgagcgg aaacacgtgg ccggggcgga
     2941 caaaatccgc agcgcccata ttgccgttgg cgagcgcccg cactgtattc gcgcgctgct
     3001 ccgcggaaat gcccgtcgtc agcccatgcc ggacgtcgac ggagactgtg aaggccgtgc
     3061 ccaatggggc atcgttcgtg gcgaccatcg gctgcagatt gagacggcgc gcctcctcgg
     3121 ggggcagcgg cgcgcagacg atgccgctcg tatggcggat gatgaagccc attttttccg
     3181 gcgtgcagag cgacgcggcg cagatgagat cgccctcatt ctcgcgatcg tcgtcatcgg
     3241 tgacgatcac gatctcgccc cgagcgatgg cctcgatcgc ctctgtcacg gtatgacgca
     3301 ttctgaaaac tccacgaatc ctgttatcta agctaaataa ggaacatcgg ccgcttggac
     3361 aatgctgcgc aaaactggtc ttgacgcgct gttgctgcgt atcgcgctcg gcgccattct
     3421 cctatcctct gcgctgacat cggcgcgcgc gcaggaaagc gggccttgca atgccatcag
     3481 cgaggccggc cagcgctata cgatctgcac cttcgatgtg cgcagagcgc ggctgcggct
     3541 cttcctggca ggtacggacg gcaagcctta tagcaatttt gcagcgatcg ccgaggcttt
     3601 gaaatcgcgt ggcgattcgc tgaccttcgc gatgaatgcc ggcatgttcg ggacggacta
     3661 tcgccccgtc gggctctatg tcgagggcgg ccgtcagctt atggcggcca acacgcgcgc
     3721 tggttcgggc aattttcacc tgaagcccaa cggcatcttt tacttcgacg caaggcacgc
     3781 cggcatcatg gagacgagcc gctaccttca agctcatctg catcctgagt acgcaacaca
     3841 gtccggcccg atgctggtga tcgacgggcg catccatccg aagatccgcc cggacggcgc
     3901 ctcggccaag attcgcaacg gcgtcggcgt cgacgaccgc ggcgcggtcg tgttcgccat
     3961 ttcggacgcg ccggtcacgt tcaacacttt tgcccggctc ttccgcgacg ggctcggcag
     4021 cggcgacgcc ctcttcctcg acggctcgat ctcgtcgctc tacgcccccg aactcaaccg
     4081 cgacgacgaa ctcatgccga tcggcccgat cgtcggcgtc gtgaaaccag agccgctggg
     4141 gcggaaccct taaggcgt
//
LOCUS       DASWOG010000599         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_235364,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000599 DASWOG010000000
VERSION     DASWOG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235364"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VGB93_12295"
     CDS             <1..645
                     /locus_tag="VGB93_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEY0146897.1"
                     /translation="MLPFAFEPGMGFERYVDYALDVPMYFVKRGDHYHDVAGASFRDL
                     LAGRLAALPGERAILSDWANHLSTIFPEVRLKRYLEIRGADAGPMPFLCALPALCFGL
                     FYDTSALDGAWDIVKNWDGEARETLRAEAPRLGLGAKIGNRLLRDIARDILALAKQGL
                     MHRAQRDAMQNDETFFLEPLEAVVAGKSHAENLIHLFKTRWNGSVDPAFSECVF"
     gene            complement(642..965)
                     /locus_tag="VGB93_12300"
     CDS             complement(642..965)
                     /locus_tag="VGB93_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0146898.1"
                     /translation="MSPGSQFRIGPVCCVSSLLFLAFTPSSFALGICAAYGPNFALVE
                     GTHTCVRIGGHMRVQFSPSAEVDPFETGGLHANATSATLRSEGFDNDASLRHMRVDTN
                     DESYR"
     gene            complement(1128..>1578)
                     /locus_tag="VGB93_12305"
     CDS             complement(1128..>1578)
                     /locus_tag="VGB93_12305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY0146899.1"
                     /translation="KAVEMGAGVLQPVLTQRTQGERLNIERMRANVVEAAEQCGILAV
                     PDVVVPVKLAALLAAWPAERTLVFCDEGADVQDPVAALSARPRPTAVAVLIGPEGGFD
                     DTERAALQSLPQVVRISLGPRILRADTAAVAALALVQAVWGDWRDGA"
BASE COUNT          317 a          475 c          472 g          314 t
ORIGIN      
        1 atgttgccat tcgcgtttga gccgggaatg ggtttcgagc gctacgttga ttacgcgctc
       61 gatgtgccga tgtatttcgt caagcgcggc gatcattatc atgatgtcgc cggtgcgagt
      121 ttccgcgatc tcctcgccgg ccggctcgcg gcgcttcccg gcgaacgggc aatcttgtcc
      181 gattgggcca accacctgtc gacgattttc ccggaggtgc ggctgaagcg ctatctcgag
      241 atccgcggtg ccgatgccgg ccctatgccg tttctctgcg cgctgcccgc gctttgtttt
      301 ggattgttct acgacacgtc ggcgctggat ggcgcctggg acatcgtcaa gaactgggat
      361 ggcgaagcgc gcgagacctt gcgggccgag gcgccgcgtc tgggtctcgg cgccaagatc
      421 ggcaatcgtc tattgcggga catcgcgcgc gacatcttgg ctctcgccaa gcagggtctc
      481 atgcaccgcg cccaacgcga tgcgatgcaa aacgacgaaa cgttttttct tgaaccgctt
      541 gaagctgtcg tcgcggggaa aagccatgct gaaaatctga tccacctttt caagacacgg
      601 tggaacggca gcgtcgatcc cgctttcagc gaatgtgtat tttagcgata cgattcgtca
      661 ttcgtatcga cacgcatgtg gcgtaggctg gcatcattat cgaagccttc actgcgcaat
      721 gttgcagatg tcgcgtttgc gtgcaggccg ccggtctcaa aaggatcaac ttcggccgaa
      781 ggcgaaaatt gcacccgcat atggccgcca attctgacgc aggtatgcgt gccttccacc
      841 agagcaaaat tgggcccgta ggcggcgcaa atgccgagcg cgaagctcga cggggtgaag
      901 gcaagaaaca gcagactcga aacgcaacag acaggaccga tacggaattg cgatccagga
      961 gacatgaaca gtcctatctt tgaattgccg agggagccgc cgcggcaatt ttccggccct
     1021 ttgtgatcgc gcgcaatcac cggacgctct gtttcaaatt cgacgacgca atagttaaga
     1081 atggtttgag gcaatatcgt ggacgccgcg ccgcaaatgt ttggctttca agcaccatcg
     1141 cgccaatcgc cccaaaccgc ctggaccaag gcaagcgccg cgacggccgc cgtatcggcc
     1201 ctcaggatac gcgggccgag cgaaattcga accacctgcg gcagcgactg aagcgcggcg
     1261 cgctctgtgt catcgaatcc gccttcggga ccgatcaaca cggccacagc cgtcggccga
     1321 gggcgtgccg acaaggctgc aacggggtct tgaacgtctg ccccctcgtc gcaaaagacg
     1381 agcgttcgct ccgccggcca tgcggcgagg agcgcagcaa gtttcaccgg cacgacgacg
     1441 tcagggacag ccagaatgcc gcattgctcg gccgcctcca cgacattggc gcgcattcgc
     1501 tcgatgttaa gccgctcgcc ctgggtacgt tgggtcagga ccggttgaag aactccggcg
     1561 cccatctcga ctgccttt
//
LOCUS       DASWOG010000600         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_235435,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000600 DASWOG010000000
VERSION     DASWOG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235435"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VGB93_12310"
     CDS             <1..1173
                     /locus_tag="VGB93_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY0146900.1"
                     /translation="DEITRLILDTHDREAFAPISHLTVGEFRNFLLSDETTPATLTAL
                     ATGITPEMAAAVSKLMRNQDLILAAKKCRVVTKFRDTIGLPGTMAVRLQPNHPTDSRA
                     GVAASIIDGLMYGSGDAVIGLNPASDSLPVLGDLLQLIDSVIARFEIPTQACILTHVT
                     SSIELINRGLPVDLVFQSVAGTEAANRSFGVDLAVLKEGRDAALSLKRGTIGDNVMYF
                     ETGQGSALSANAHHGVDQQTLEARAYATCRPFKPLLVNTVVGFIGPEYLYDGKQIIRA
                     GLEDHFCGKLMGLPLGCDVCYTNHAEADQDDMDNLMTLLAAAGITYIMGIPGADDVML
                     NYQSTSFHDALYLREVFGLKRAPEFEAWLQRMQIADGAGRLMRQEVAHPLLAWHEG"
     gene            1175..>1642
                     /locus_tag="VGB93_12315"
     CDS             1175..>1642
                     /locus_tag="VGB93_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEY0146901.1"
                     /translation="MRPPVVEDFWKRLASTTQARIALGRAGVSLPTQEVLNFSLAHAR
                     ARDAVHTPFDHDKIAAEITDLGFATICVESEAPDRAAYLRRPDWGRTLSEKSRALLAD
                     RQNEPVDISIVVADGLSATAVHSQAVPLLAAFKPYLIRAGWRIAPVVIASQARV"
BASE COUNT          308 a          507 c          502 g          325 t
ORIGIN      
        1 gatgagatca cgcggctcat tctcgatacg cacgacagag aggccttcgc gccgatctcg
       61 catctcaccg ttggcgaatt ccgcaatttc ctgctctcgg acgaaacaac gcccgccacg
      121 ctcaccgcgc tggcgacggg catcaccccg gaaatggccg cggccgtcag caagctcatg
      181 cgcaatcagg atctgatcct ggcggcaaag aaatgccgcg ttgtgacgaa attccgcgac
      241 acgatcgggc tgccgggcac gatggcggtg cggttgcagc ctaatcatcc gaccgacagc
      301 cgcgccggcg tggcggcctc gatcatcgac gggctgatgt atggcagcgg cgatgccgtg
      361 atcggcctca atcccgcctc cgacagtctg cctgttcttg gcgatctgct gcaattgatc
      421 gacagcgtta tcgcgcggtt tgaaatcccg acgcaggcct gcatattgac gcatgtgacg
      481 agctcgatcg aactcatcaa tcgcggcctg ccggtcgatc tcgttttcca gtcggtcgcg
      541 ggcacggaag ctgcaaaccg ttcgttcggc gtcgatctag ccgtactgaa ggaaggccgc
      601 gacgccgcgc tgtcgctcaa acgcggcacc atcggcgaca atgtgatgta tttcgaaacc
      661 gggcagggca gcgctttatc ggccaacgcc catcacggcg tcgatcagca gacgctggag
      721 gcgcgcgcct atgcgacatg ccggccgttc aagccgctgc tcgtcaacac ggtcgtcggc
      781 ttcatcgggc cggaatatct ttatgatggc aagcagatca tccgtgccgg gctcgaagat
      841 catttctgcg gcaagctgat gggcctgccg cttggctgcg acgtctgcta cacgaaccac
      901 gccgaggcgg accaggacga catggataat ctgatgacac tgctcgcagc tgcgggaatc
      961 acttacatca tgggcattcc cggcgccgac gacgtcatgc tcaattatca atcgacctca
     1021 tttcacgatg cgctttattt gcgtgaggtc ttcgggctca agcgagcgcc ggagtttgag
     1081 gcgtggctgc aacgcatgca gatcgccgat ggcgcggggc gccttatgcg gcaggaagtg
     1141 gcgcatcctt tgctggcctg gcatgaaggc taacatgcgt ccgccagtcg ttgaggattt
     1201 ttggaaaagg ctcgcttcga cgacgcaggc acggattgcg ctggggcggg ctggcgtctc
     1261 gttaccgaca caagaggtcc tgaatttttc gctcgcgcat gcccgggcgc gcgatgctgt
     1321 gcacacgccc ttcgaccacg ataagatagc cgccgaaatc accgacctcg gatttgcgac
     1381 gatctgcgtc gagagcgaag caccggatcg agccgcctat ctgcgccggc cggattgggg
     1441 acgtacgctc agtgagaaaa gccgggcttt gctcgcggat cgtcagaacg agcctgtcga
     1501 catctcgatc gttgtcgcag atggcctgtc cgccacggcg gttcatagtc aggctgtgcc
     1561 gttgcttgcc gcgttcaagc cttatctcat ccgcgccggc tggcgcatcg cgccggtcgt
     1621 tatcgcaagc caggcgcgcg tc
//
LOCUS       DASWOG010000601         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_15102,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000601 DASWOG010000000
VERSION     DASWOG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15102"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..991
                     /locus_tag="VGB93_12320"
     CDS             20..991
                     /locus_tag="VGB93_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY0146902.1"
                     /translation="MKQFSQLSEREILALAVDAEDDDHRVYSSFAEDLHERYPGTAKI
                     FADMADVEASHRDALLKTYREKFGPNLLPIRRADVRGFMKRRPIWLVRNLPLSQIRTE
                     VDFRETEAYNFYVKAAKQAQDPAIKTLLADLAEVEKGHEAIAADIQDEYLNPAEREKE
                     DARQKRNFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHNNWSTFLVGLAASIGAGIS
                     MAFAEALSDDGAISGRGSPWLRGPICGAMTALGGLGHTLPFLVPDSLPHAFTTATAIS
                     GVVVFFELWGIAWVRAHYMDTPLLKAIFQIVLGGVIVLVAGILIGNA"
     gene            1011..1892
                     /locus_tag="VGB93_12325"
     CDS             1011..1892
                     /locus_tag="VGB93_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0146903.1"
                     /translation="MTLRLAHLSDAHIGPLPRPRKHELFGKRLTGYLNWTRGRHLAHD
                     MDALGVLVADIKAQRPGHIAMTGDILNIGLKAEFPLARAWLETLGGPKDVSFVPGNHD
                     AYVRAALPYLASTFAPWSRGDAGETFPYLRVREGVALIGLSSAVPTLPLIASGRLGRP
                     QLARAAELLRQTAQDGLIRVVMIHHPPHQGGAKFARGLSDAAQFEAMIRRVGAELVLH
                     GHNHKLSIAHLPGPSRQVPVIGVASASAIHDSGAYRASYNFFQIEGTQKSPYIRARMR
                     GLLPGGREVGDLRRIVI"
     gene            complement(1901..2350)
                     /locus_tag="VGB93_12330"
     CDS             complement(1901..2350)
                     /locus_tag="VGB93_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0146904.1"
                     /translation="MREELTSELKTAMKAGVKRRVDTIRLINAALKDRDIEARGQGKT
                     VPDDDILGLLQKLIKSRQESVEIYDKAGRIDLATQEREEIAVIQTFLPQPLSAEEADA
                     AISAAIAETGASSIKDIGRVIGVLRANYAGRMDIAQASAAVKAKLGG"
BASE COUNT          432 a          793 c          744 g          516 t
ORIGIN      
        1 tctagaaaat aggccgtcca tgaaacagtt ctcgcaactc agcgagcgcg aaatccttgc
       61 tttggctgtc gatgccgagg acgacgacca ccgcgtctat tcgtcgttcg ctgaggattt
      121 gcacgaacgc tatccgggca cggcgaaaat attcgctgac atggccgatg tcgaggcgtc
      181 gcatcgcgac gcgctgctca agacctatcg cgagaaattc ggccctaatc tcctgccgat
      241 ccggcgtgcg gacgtccgtg gcttcatgaa gcggcgccca atctggctag tccgcaatct
      301 gccgctctcg caaatccgca ccgaggtcga tttccgcgaa accgaagcct acaattttta
      361 cgtcaaggcg gcgaagcagg cgcaagatcc agccatcaaa acattgctcg ccgatctcgc
      421 ggaagtcgaa aaaggccacg aagccatcgc ggccgacatt caggacgaat atctgaatcc
      481 cgctgaacgc gagaaggagg atgcgcggca aaagcgcaat ttcgttctgc aatatgttca
      541 gccgggtctc gccgggctga tggacgggtc ggtctcgacg ctggcgccgc tcttcgccgc
      601 ggctttcgcc acgcataaca attggtcaac gttcctcgtc ggccttgccg cctcaatcgg
      661 cgccgggatc agcatggcat tcgccgaggc gctttcggac gatggcgcca tcagcgggcg
      721 tggctccccc tggctgcgcg gcccgatctg cggcgcgatg acggcgctgg gcggcctcgg
      781 ccatacgctg ccctttctcg tgcccgattc actgccgcac gctttcacca ccgcaacggc
      841 gatttcaggc gtggtcgttt tcttcgaact ctgggggatt gcttgggtgc gtgcccatta
      901 catggacacc cccctcctaa aagcgatatt ccagatcgtg ctcggcggtg tcatcgtgct
      961 cgtggccggc attcttatcg gcaacgccta aagttcgtcc ggagatttgt ttgacgttac
     1021 ggcttgccca tctgtccgat gcgcatatcg gacctttgcc gcgcccgcgg aaacatgaac
     1081 ttttcggcaa gcggctgacc ggctatctca attggacgcg gggccgccac ctcgcccacg
     1141 acatggatgc gctcggtgtt ctcgtcgctg acatcaaggc gcagcgcccc ggccatatcg
     1201 ccatgaccgg cgacattctc aacatcggcc tcaaggccga atttccgctg gcgcgcgcct
     1261 ggctcgaaac gctgggcggt cccaaggatg tcagcttcgt gcccggcaat cacgatgcct
     1321 atgtgcgcgc ggcgctgccg tatcttgcgt cgacctttgc accatggagc cggggcgatg
     1381 ccggcgaaac ctttccctat ttgcgggtgc gcgagggggt ggcgttgatt ggcctttcct
     1441 cggcggtgcc gaccttaccg ctgatcgctt cgggacggtt gggccgtcct caattggcga
     1501 gggcggccga gctcttaagg caaaccgcgc aagacgggct gatccgcgtg gtgatgatcc
     1561 atcatccgcc gcatcaaggc ggcgcgaaat tcgcccgtgg tctatccgac gcggcccaat
     1621 tcgaggcgat gatccgccgg gtcggcgccg agcttgttct ccacggccac aaccacaagc
     1681 tttcgattgc gcatctgccg ggaccgtcac ggcaagtccc ggtcattggg gtcgcctccg
     1741 cctcggccat tcacgattcc ggcgcctaca gggccagtta caatttcttc cagatcgagg
     1801 gcacgcagaa gagcccttac atcagggcgc gaatgcgcgg cctgctgcca ggcggccgcg
     1861 aggtcggtga tctgcggcgg atcgtgatct gaggcaggct ttagccgccc agtttcgctt
     1921 tcacggcggc gctggcctgg gcgatgtcca tccggccagc atagttggcc cgcagcacgc
     1981 cgatgactct gccaatatcc ttgatcgaac tcgcgcctgt ctcggcgatc gccgcactga
     2041 tcgcggcatc tgcctcttct gcgctcaagg gttgcggcag gaacgtctgg atgacggcga
     2101 tttcttcccg ctcctgcgtc gcgagatcga tgcggccggc cttgtcgtag atttcgaccg
     2161 attcctggcg gcttttaatg agtttctgca gcagccccag aatgtcgtcg tccgggacgg
     2221 tcttgccttg gccgcgcgcc tcgatatcgc gatccttcag cgcggcattg atgagccgga
     2281 tggtgtcgac gcggcgcttc acgccggcct tcatcgccgt cttcaattcg ctcgtcagtt
     2341 cctcgcgtat cgccacgttc gatcctatct agggaatatc tggattgacc agcgccgctc
     2401 agcgggccac ttaggccgcg aggcgcgatg gagggtgtaa agcaggcatg accgggcagc
     2461 aagacaaaca aggctggacc attcc
//
LOCUS       DASWOG010000602         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_50798,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000602 DASWOG010000000
VERSION     DASWOG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50798"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VGB93_12335"
     CDS             complement(<1..886)
                     /locus_tag="VGB93_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEY0146905.1"
                     /translation="MQRLSLRWRLNLLLGAMLFLSLLVNFALIGWNAWPRIAAEGESD
                     EVLARELVETALASLQEAPDPEPPLRRLLAQLQDLRHVRILVAADDAQAQTLPLTLYT
                     SPSGGAPAWFLNLFQQRPSVTIVPATIHGRNYGDIVIAANPTNEVTEIWGEVCSVAAT
                     TLAFGVCLFGLILLLVGRAIAPIADVGNAITRLARGDTEIKLVPRGPPEFVDIGNKIN
                     GLAVNLAKVSAENHRLIHQMMRVQEEERGQIARDLHDEMGPPLFCIRANVSALSGSLP
                     AAPVIRQNVTSIGEQAEAI"
     gene            1120..1737
                     /locus_tag="VGB93_12340"
     CDS             1120..1737
                     /locus_tag="VGB93_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0146906.1"
                     /translation="MKILIVDDHPVILSGCRAMLSPYSDFEAFDALDATQAYDEYVEK
                     RPDVVVIDINLPGMSGFALTRRILDFDPQAKIIIFTMNDDPIFAARAIENGAKGYIGK
                     CEDPARFVAAIRAVAEGQTFLLPEMAQKLAFLDPRRRDFLTGLNPRELEILRLLRSGK
                     SMAEIAGIINVSYKTVANSCAVLKRKLGARAPLDLVRIAVENKLA"
     gene            complement(1776..2228)
                     /locus_tag="VGB93_12345"
     CDS             complement(1776..2228)
                     /locus_tag="VGB93_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146907.1"
                     /translation="MLPENKTKLASLALAGALAVGFTGAASAQDWSQTTYRETHHAMH
                     YRYSTPQRYDYGYHREGYYRDGYDDGYRDGPVGAAGAIVGSALGAADTAAAVATGFPN
                     GYGYYGPGPYYAPAAYYNGYSPDYGNDWWGPYHNQYQNGYPQYQNGNW"
     gene            complement(2407..3360)
                     /locus_tag="VGB93_12350"
     CDS             complement(2407..3360)
                     /locus_tag="VGB93_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0146908.1"
                     /translation="MADTKHYNIVFLDRATIGVPVRQPNFPHSYKEYEETVADQVVER
                     LADADIAIINKVQVRAASLEKLPKLKLIAVAATGTDCVDKAYCKAHNIIVSNIRNYAD
                     NTVPEHTLALIFALRRSLVPYVQDVRRGKWQTINQFCYFDHPIRDIAGSTLGLIGYGA
                     LGKSVGARAEALGMKVIATDLYDFPGKVDLDTVLRESDVISLHCPLTEQTRNVIGAAE
                     LKKMKNDAILINTARGGLVDEKALAEALKSGEIGGAGFDVLTVEPPKNGNVLLDADLP
                     NLLITPHVAWASVEAMTGLSNQLVDNIEAWAAGKPRNLVLE"
     gene            complement(3386..4090)
                     /locus_tag="VGB93_12355"
     CDS             complement(3386..4090)
                     /locus_tag="VGB93_12355"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY0146909.1"
                     /translation="MPPIPSAALAPVVAAPAPFVPAPDPTCPAVLQSKEIVVAAAPAV
                     SGPDGCGIAAPVTLSAVILKDGTKVPFEPPSLIRCDLAEMLGTWFREDVAPALETAGG
                     LAKILGSNGYECRNRDHLVRAKLSEHAKGNALDLRGFVLRNGQTILIEKNAPQPAFLT
                     QIRATACTRFRTVLGPGGDVFHETHLHVDLEQRHHDFRICEWVVGPPAFDPKMPVQGP
                     KMQKAGSGIDVQGPHT"
     gene            complement(4325..4813)
                     /locus_tag="VGB93_12360"
     CDS             complement(4325..4813)
                     /locus_tag="VGB93_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0146910.1"
                     /translation="MTINVGDALPQVTFTVMTADGPQPRTTNEIFAGRRVALVGVPGA
                     FTPTCSLNHVPGFIEKESDLHDKGVALIAVTSVNDVFVMSAWAKSLGADPEGFVFLAD
                     GSAVFAKAIGLTLDLADRGLGLRSQRYAMLVDDGVVTQLNIEQASGKADVSSAEALLK
                     QL"
     gene            5070..5222
                     /locus_tag="VGB93_12365"
                     /pseudo
     CDS             5070..5222
                     /locus_tag="VGB93_12365"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
     gene            5229..5393
                     /locus_tag="VGB93_12370"
     CDS             5229..5393
                     /locus_tag="VGB93_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEY0146911.1"
                     /translation="MPDEKRSFSQNHELASEAGRKGGQMSGGNFANDRARAAEAGRKG
                     GEASHGNRHQ"
     gene            complement(5447..6061)
                     /locus_tag="VGB93_12375"
     CDS             complement(5447..6061)
                     /locus_tag="VGB93_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0146912.1"
                     /translation="MIRADPAHRLHDVAEEAARCTRCPLYRDATQVVLGEGPANAAIM
                     LVGEQPGDKEDIAGKPFVGPAGHVLDQALAQAGLDRATCYVTNAVKHFKHEQRGKFRL
                     HKQPTRGEIQACHWWLDRELEIVQPKLIVALGVTAASSLKGKPVVLSRLRRQILDVEP
                     WRALATTHPSAILRLRDADEKEHAFRELVDDLRFALAFLKQPEI"
     gene            complement(6058..6957)
                     /locus_tag="VGB93_12380"
     CDS             complement(6058..6957)
                     /locus_tag="VGB93_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0146913.1"
                     /translation="MGQDAGIWIGISGWTYAPWRGQFFPKSLPQKQELAYASSFFRAI
                     EVNGTFYGLQRPDMYRVWASQVPDNFLFAVKAPRFITHVKRGRDALVPIANFFASGVL
                     ALGPKLGPILWQFPPSFQFNRDQFTEFLGHLPHDTKAASALAGRHDDHLKGRAWLKTD
                     TTRPVRHAVEIRHKSFIDGAFIDLLRAHDVALVCADTVEWPRLMDMTADFVYCRLHGS
                     TELYRSGYDDTALDEWAARIRKWTSGKAMDDGDFAAPAHGGAKRRDVFVFFDNTDKLH
                     APTDALTLMKKLGIATGHEDRAA"
BASE COUNT         1504 a         2106 c         2117 g         1403 t
ORIGIN      
        1 gaattgcttc cgcctgctcg ccgatggagg tgacattctg cctgatgaca ggtgccgctg
       61 gcagactgcc gctgagcgca ctgacattcg cgcggatgca aaacaggggc ggtcccatct
      121 cgtcgtggag atcgcgggcg atctggccgc gctcctcctc ctgcacgcgc atcatctgat
      181 gaatcaaacg atggttctcg gcactgacct tggccagatt gacggcgagc ccgttgatct
      241 tgttcccgat gtcaacgaac tccggcggcc cgcgcggtac gagcttgatt tcggtgtcgc
      301 cgcgcgcaag ccgtgtgatc gcattgccga catcggcgat cggcgcaatt gcgcggccga
      361 cgagaagcag gatcagcccg aaaagacaaa ccccgaaagc gagcgtcgtc gcggcgaccg
      421 aacagacctc accccagatt tcagtgacct cgttcgtggg gttggcggcg atgacgatgt
      481 cgccgtaatt gcggccgtgg atcgtcgccg gaacaatggt gacactcggg cgctgctgaa
      541 acaggttcag gaaccacgcc ggcgcaccgc ccgacgggct tgtatataaa gtgagcggca
      601 gggtctgggc ctgggcatca tctgccgcga ccaggatgcg cacgtgccgc aagtcctgca
      661 attgcgcgag caggcgccgc aacggcggct cggggtcggg agcttcctgc aaactcgcaa
      721 gcgccgtctc gaccaattcg cgcgccaata cctcgtcgct ctcaccttct gctgcaatgc
      781 gcggccacgc attccagcct atcaaggcaa aattgaccag aagcgagaga aaaagcatgg
      841 cgccgagaag aaggttcaag cgccagcgca aggaaagtct ctgcattttc acgccccatt
      901 cccgccgcag gccgaccctg cactcatgtt cggcactgga aagcccaaac attcctcctg
      961 aattcgggag tatttgctat tgcaagcgtg atattttcta tgcggaaaag catagacgtc
     1021 cgaattgtcc tgctaaccgt ctaagatatc agcgaaaatc gttgacgtat attgcccagg
     1081 atcgcaagat cgccgccgtt ccaagccgga tcggagaaaa tgaagattct tatcgtcgat
     1141 gatcaccccg tcatcctctc aggatgtcgc gcgatgctct cgccatactc ggattttgaa
     1201 gcattcgatg cgctcgatgc aacgcaagcc tacgatgaat atgtcgagaa acggccagat
     1261 gttgtcgtga tcgacatcaa cctgccaggc atgtctggct ttgcgctcac gcgccggatc
     1321 ctcgatttcg acccgcaggc gaagatcatc attttcacga tgaacgacga tccgatcttt
     1381 gccgcacgtg caatcgaaaa cggcgccaaa ggctatatcg gcaagtgtga agatccggca
     1441 cgtttcgtcg ccgcgatccg cgccgtcgca gaaggccaga ccttcctatt gcctgaaatg
     1501 gcgcaaaagc tggcgttcct tgatccgcgg cggcgggatt ttttgaccgg cctcaatccg
     1561 cgcgaattgg aaattttgcg gctgctgcgc agcggcaaaa gcatggccga gatcgcgggc
     1621 atcatcaacg tgtcttacaa aacggtcgca aattcctgcg ctgtcttgaa gcgcaaactc
     1681 ggcgcccgcg cgccgctcga tcttgtccgc atcgcggtcg agaataaatt ggcgtgaccc
     1741 tccccggtct tgccggagag agatcatcat caaacctacc aatttccgtt ttgatattgg
     1801 gggtagccgt tctgatactg gttgtggtac ggcccccacc aatcgttgcc gtagtctggg
     1861 ctatagccat tgtaataggc ggcgggggcg taatacgggc cggggccata atagccgtag
     1921 ccattaggaa aaccggtcgc aaccgcggcg gccgtgtccg ccgcgcctag cgcagaacca
     1981 acaatcgcac cagccgcgcc gaccgggcca tcgcgatagc catcatcgta gccgtcgcgg
     2041 taataccctt cgcggtgata gccatagtca tagcgctgcg gcgttgaata gcgataatgc
     2101 atcgcgtgat gcgtttcgcg ataggtcgtc tgtgaccagt cctgcgctga tgccgcgcca
     2161 gtgaacccga ccgcaagcgc gccggccaat gccaagcttg cgagcttcgt tttgttttct
     2221 ggtagcattc aagcctcctg ttctattcag gcgatacgca gccgttaccg gcagcgctcg
     2281 attgcccgac cgacttacgc cgaacgacaa gaatcgttcc ggcagcggcg gaggcgtagc
     2341 gcgaaaaaaa ggcgccgcac agggagcgcg gtgccaaata attgagagat aaagaaaagc
     2401 cgacgcttat tcgagcacga gattgcgcgg cttgcccgcg gcccaagcct caatgttgtc
     2461 gacgagctgg ttggaaagcc ccgtcatcgc ttcgacgctg gcccacgcca catgcggtgt
     2521 gatcagcagg ttcggcagat cggcatcgag caggacattg ccgttctttg gcggctcgac
     2581 tgtcaacacg tcgaaacccg cgccgccaat ctcacccgat ttcaatgcct cggctaaagc
     2641 cttttcgtcg acaaggccgc cacgcgcggt gttgatgaga atggcatcgt tcttcatttt
     2701 cttgagttcg gcggcgccga tcacattgcg cgtctgctcg gtgagcgggc aatgcaacga
     2761 aatgacatcg ctctcgcgca acaccgtatc gagatcgacc ttgccgggga aatcatagag
     2821 atcggtagcg ataaccttca tgcccaaagc ctcggcgcgc gcgccgaccg atttgccgag
     2881 cgcgccatag ccgatgaggc ccagcgtcga accggcaatg tcgcggatcg ggtgatcgaa
     2941 atagcagaat tggtttatcg tctgccactt gccacggcgc acgtcctgca cataagggac
     3001 gaggctgcgg cgcagtgcga agatcaacgc gagcgtatgt tccggcaccg tattgtcggc
     3061 ataattgcgg atattcgaaa caatgatgtt gtgcgccttg caataggcct tgtccacgca
     3121 atcggtgccg gtcgcggcga cagcaatcaa tttcagcttc ggcagctttt cgaggctcgc
     3181 ggcgcgtacc tgcaccttgt tgatgatcgc gatgtcagcg tcggccagac gctcaaccac
     3241 ctggtcggcg accgtctcct cgtattcctt gtagctatgc ggaaaattcg gctgtcgcac
     3301 gggaacgccg atcgtcgcac ggtcgaggaa aacgatattg taatgctttg tgtcggccac
     3361 gggcgcctct ccttttcgcc cttccttatg tgtgcgggcc ttgaacgtca attccggacc
     3421 ccgccttttg cattttaggg ccttgcacag gcattttagg gtcgaacgca ggaggcccga
     3481 cgacccattc gcagatgcga aaatcatgat ggcgctgttc gagatcgacg tggagatgcg
     3541 tctcgtggaa cacgtcgccg ccggggccga gaacggtcct gaagcgggtg caagccgtcg
     3601 ccctaatttg cgttagaaat gccggttgcg gggcgttttt ttcgatcaga attgtttgcc
     3661 cgttgcgcag gacaaagccg cgcagatcga gggcattgcc cttggcgtgt tcactgagtt
     3721 tcgcgcggac caaatgatcg cggttgcggc attcgtaacc gttcgatcca aggattttgg
     3781 cgaggccgcc cgcggtttct agcgctggcg cgacatcttc gcgaaaccat gtccccagca
     3841 tctccgccag gtcacagcgg atcagcgacg gcggctcgaa cggcactttc gtcccatcct
     3901 tgaggatgac ggccgacaag gtaacgggcg ccgcgatgcc gcagccgtcg ggaccactga
     3961 cggccggcgc cgcggcaaca acaatctcct tgctttgcag gacggcggga cacgtgggat
     4021 cgggcgccgg gacaaatggc gcgggcgctg ccacgaccgg cgcgagcgct gccgaaggta
     4081 tcgggggcat gggttgcgcc ggtgctgggg tgggcgatgt gatatctgcc gggcggctcg
     4141 gtggcagcgg cgggccatcc tgttcgccaa aatcatcggg tcgcggcagc ggaaatggtg
     4201 caaccgcgaa atggcgcgcc gatgcaccat cggtaagcag tccggaaaag agaaacgcca
     4261 aaagaaaagc cggacacgac cgcatccgac ttcaacgcgg caaatcgccg aaagctgctt
     4321 caaattagag ttgcttcagc agagcctcgg cgctcgacac atcggctttg ccggatgcct
     4381 gttcgatgtt gagctgcgtc accacgccat catcgaccag catggcgtag cgttgcgagc
     4441 gcagaccaag gccgcgatca gcgagatcaa gcgtgagacc gatggctttg gcaaaaaccg
     4501 cgctgccgtc ggcgaggaaa acaaagcctt cgggatcggc gccgagcgac tttgcccatg
     4561 cgctcatcac gaaaacatcg ttgaccgacg tgaccgcgat cagagcgacg cctttatcat
     4621 gcaggtcgct ttccttttca atgaagcccg gcacgtgatt cagcgaacac gtcggcgtaa
     4681 aagcgcccgg cacgccgaca agcgcaacgc ggcgcccggc gaaaatctcg tttgtcgtgc
     4741 gcggctgcgg accatcggcc gtcatcacgg tgaaagtcac ttgcggtaat gcgtcaccta
     4801 cgttgattgt catattggcc cctttgtttc gtcgcggtcg aaattagccg ctcttgcgac
     4861 aatctcaagt cagcgtgagc ggaagtcgtc tcaagttgcc gtgtgaccct cgtttgcagc
     4921 gtcatgacga cgattcatgt gatgattcaa gcgaagcggc ggcaacgcct tcaatattaa
     4981 gcatcgttaa tttcggaaca agccttggcg acacacgttc gctgggcagc cggtggttcc
     5041 ggcaatcgtc catttctagg gagaacatta tggcgaatac gacctcgaac cggggcttcg
     5101 cttcgatgga tggagagaag caacgtgaga tcgcgcgcaa aggcggcgcc agcgttccag
     5161 ctgaagagcg cagcttctcg cgtgaccacg agcttgccgc ggaggctggc cggaagggcg
     5221 gcgccagcgt gcccgatgaa aagcgcagct tctctcaaaa ccatgagctt gcatccgagg
     5281 ctggccgcaa gggtggccag atgtccggcg gcaatttcgc gaatgatcgt gcgcgtgcag
     5341 ccgaggctgg acgtaagggc ggcgaggcct cgcacggcaa ccgtcaccaa taggtagcga
     5401 atcgatcttt gatggcagcc gggatcacca atcccggctg ccaaattcat atttcaggct
     5461 gctttaggaa tgctaacgcg aagcgaagat catcgacgag ctcgcgaaac gcgtgctctt
     5521 tttcgtcagc atcacgaagg cgcaaaatcg ccgacggatg agtggtcgca agcgctcgcc
     5581 atggctcgac atccaggatt tgccgccgca accgcgaaag cacgaccggc ttacccttca
     5641 gcgagctcgc ggccgtgacg ccgagggcga caataagttt cggctggaca atctctagtt
     5701 cacgatcaag ccaccaatga catgcctgga tttcgccgcg cgtcggctgt ttgtgcagcc
     5761 ggaacttgcc acgctgttca tgtttgaaat gcttgaccgc atttgtgaca taacaggtgg
     5821 cgcggtcgag tccggcctga gcaagcgctt gatcgagcac atgccctgct ggcccgacga
     5881 agggtttgcc ggcgatatct tctttgtcgc ccggctgttc gccgacgagc atgatcgcgg
     5941 catttgccgg gccttcgccc aagacaacct gcgtcgcatc gcgataaagc ggacagcgcg
     6001 tgcaacgcgc ggcctcttcg gcaacatcgt gcaagcggtg cgcgggatcc gcgcgtatca
     6061 tgcggcgcga tcctcatgcc ccgtagcgat tccgagtttt ttcatcaacg tcagggcgtc
     6121 ggtcggcgcg tgcaatttgt ccgtattgtc gaagaagaca aaaacatcgc ggcgctttgc
     6181 acccccatgc gcgggcgcgg caaaatcacc atcatccatg gctttgccgg aagtccattt
     6241 gcgaatgcgc gcggcccatt cgtcgagcgc cgtatcgtca tagccgctgc gataaagctc
     6301 agtcgatccg tgcaggcggc aatagacaaa atcggcggtc atatccatga gacgcggcca
     6361 ttcgaccgtg tcggcacaga ccaaggcgac atcgtgtgcg cgcaagaggt caataaaagc
     6421 cccgtcgata aagctcttat ggcgaatctc gacggcatgc cggactggcc gcgtcgtatc
     6481 cgtctttagc caggcgcgac ctttgagatg atcgtcatgt cggcctgcca acgccgacgc
     6541 cgccttggtg tcatgcggca gatgcccaag gaattcggtg aactggtctc gattgaactg
     6601 gaagctcggg ggaaactgcc aaaggatcgg gccgagcttc ggcccaagcg cgagcacgcc
     6661 ggacgcgaag aaattagcaa ttgggacgag cgcatcgcgc ccgcgtttga catgcgtgat
     6721 gaagcgcggc gccttgaccg caaatagaaa gttatccggt acctgcgacg cccagacgcg
     6781 atacatatcc ggcctttgca gaccatagaa ggtgccgttg acctcgatgg cgcgaaaaaa
     6841 cgaactcgca taggcaagtt cctgcttctg cggcagactt ttcggaaaga actggccgcg
     6901 ccacggcgca taggtccagc ccgagattcc gatccagata cccgcgtcct ggcccatctc
     6961 caccgcaccg tcgcgtagcc gcacagctgg agaacgtgcg acgggctatt ttgctcccga
     7021 cgtcgaagct tgagccggta aatcgagata cacgatgaag acgtaagacc ggcccgtagt
     7081 cctcagggtc agatgcgctg ggatgcgttg cgacagccct gggaacgacc
//
LOCUS       DASWOG010000603         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_198909,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000603 DASWOG010000000
VERSION     DASWOG010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198909"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VGB93_12385"
     CDS             complement(<1..174)
                     /locus_tag="VGB93_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY0146914.1"
                     /translation="MRIPCPYCGFRDLSEFVTFGEATGPRPDPAAPDAAEKFFDYAYL
                     RTNPAGPSEEYWYH"
     gene            complement(247..1497)
                     /locus_tag="VGB93_12390"
     CDS             complement(247..1497)
                     /locus_tag="VGB93_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY0146915.1"
                     /translation="MHYSFLSLLKNAFTDHQGWTPTWRDAQPKPEYDAVIVGGGGHGL
                     STAYYLAKEHGLTNVAVLEKGWIGGGNIGRNTTIIRSNYLLPGNIVFYEWSMKLWEKL
                     EQDFNYNAMVSQRGVLNLYHSDGQRDAYARRGNAMRLHGVDADLLDREQVRAMLPFLN
                     FDDARFPIQGGLLQKRGGTARHDAVAWGYARGASDRGVDIVQNCEVTGLTIENGRVTG
                     VETTRGPIRAKKVGLSVAGNSSRVAAFAGLRLPIESHVIQAFVSEGLKPLIPNVITFG
                     AGHFYISQSDKGGLVFGGDLDGYKSYAQRGNLPTIKDVCEGGMALMPMIGRLRVLRNW
                     GGIMDMSADGSPIIDKTPVEGLYLNTGWNYGGFKAVPAAGWCFAHLIARDETHSAAAA
                     FRLDRFARGAVLDEKGQGPQPNLH"
     gene            1569..>2113
                     /locus_tag="VGB93_12395"
     CDS             1569..>2113
                     /locus_tag="VGB93_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0146916.1"
                     /translation="MTKSAISMTKGDISVGQRDEVRKLGFLLIDDFALMSFASAIEPF
                     RAANVLAGRSLYEWVAIGVHRNGAAASAELLVQTQHLVGADLDLDTLFVCAGGNPAEF
                     ADEATLQWLRRLARRGVRIGGVSGGPFILARAGLLDGYRCTIHWEHVPAFRESFPQLA
                     LRRSLYEIDRDRLTCAGGIAAL"
BASE COUNT          406 a          626 c          658 g          423 t
ORIGIN      
        1 atgataccaa tattcctcgc tcgggccggc ggggttggtg cgcagataag catagtcgaa
       61 gaatttctcc gccgcgtctg gcgctgcggg atcggggcgc gggcctgtcg cttcgccgaa
      121 ggtgacgaat tcggaaaggt cgcggaaacc gcaatagggg caggggatac gcatggcgtc
      181 tacatcacga tggtcttaga gcgggattga aacaaaaaac cggtctccac ttttttgcat
      241 cccgctctaa tgcagattgg gttgcgggcc ttggcccttt tcgtcgagga ccgcgccgcg
      301 ggcgaaacga tcgagtcgga aggctgcggc ggcggaatgc gtttcgtctc gggcaatcag
      361 atgagcgaag caccagcccg ccgccggcac ggctttgaag ccgccgtagt tccagccagt
      421 gttgagatag aggccttcga ccggcgtctt gtcgatgatc ggcgaaccat ccgccgacat
      481 gtccatgatg ccaccccagt tgcgcagaac gcgcaggcgg ccaatcatcg gcatcaatgc
      541 catgccgcct tcgcagacat ccttgatggt cggcagattg ccgcgctggg cataggactt
      601 gtagccgtcg agatcgccgc cgaagacgag gccgcccttg tcggattggc tgatgtagaa
      661 atgaccagca ccgaacgtga tgacgttcgg aatgagaggc tttaaacctt ccgaaacgaa
      721 agcctgaatg acatggcttt cgatcggcag gcgcaggccg gcgaaggccg caacgcgcga
      781 gctgttgccg gcaacggaaa gaccgacctt tttggcgcgg atggggccgc gcgtcgtctc
      841 aacgccggtg acgcggccgt tctcgatggt caggcccgtc acttcgcaat tctgcacgat
      901 atcgacgccg cggtcgctgg caccgcgcgc ataaccccag gcaacggcat cgtgccgtgc
      961 cgtgccgcca cgcttctgca aaaggccacc ttgaatcggg aagcgcgcat catcgaaatt
     1021 cagaaatggc agcatggcgc gcacctgctc gcggtcgagc aagtccgcat cgacgccgtg
     1081 gagccgcatc gcattgccgc gccgcgcata ggcgtcgcgc tggccatcgg aatgatagag
     1141 attcaacacg ccgcgttggc tgaccatcgc attgtagttg aaatcctgct ccagtttttc
     1201 ccagagcttc atcgaccatt cgtagaagac gatattgccg ggcaacagat agtttgaacg
     1261 gataatcgtt gtgttgcggc cgatatttcc accgccgatc cagccttttt ccaacaccgc
     1321 gacattggtg aggccgtgct ctttggcgag ataataagca gtcgaaagac cgtggccgcc
     1381 gccgccgacg atgacggcgt catattccgg cttgggttgg gcgtcgcgcc acgtcggcgt
     1441 ccagccttga tggtcggtga aggcgttttt cagcagggac aagaaagaat aatgcatgtc
     1501 gatcgcaaag gggcggcggc gctgcggcct tgacttcggc cagcatggac gcggataaat
     1561 ggaaaatctt gaccaagagc gccatttcca tgaccaaagg cgacatatcc gtggggcagc
     1621 gcgatgaggt gcgaaagctg ggctttctgc tgatcgacga tttcgcgctg atgtcatttg
     1681 cgtccgcgat tgagccgttc cgcgctgcca atgtgctcgc gggccgcagc ctctacgagt
     1741 gggtcgcgat cggtgtccat cgaaacggcg cagccgcgtc cgccgaactc ttggtgcaaa
     1801 cgcagcatct tgtgggtgcc gatctcgatc tcgatacgct ttttgtttgc gccggcggca
     1861 atccggcgga atttgccgac gaggcgacat tgcaatggct tcggcgtctg gcgcgccgcg
     1921 gcgtgcggat cggcggcgtc tccggcggcc ctttcattct ggcccgcgcc ggccttctcg
     1981 acggctaccg ctgtaccatt cactgggaac acgttccggc tttccgcgaa agctttccgc
     2041 agctcgcatt acgccgctct ctctatgaga tcgaccgcga caggctcacg tgcgccggcg
     2101 gcattgccgc gct
//
LOCUS       DASWOG010000604         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_344197,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000604 DASWOG010000000
VERSION     DASWOG010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344197"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1357
                     /locus_tag="VGB93_12400"
     CDS             161..1357
                     /locus_tag="VGB93_12400"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEY0146917.1"
                     /translation="MSKTTDLVILVVAAGRGTRAGRGLPKQYRPLAGKPLLGHTLFTL
                     LQAAPEARVLAAIHPDDVEFYKQTAADLPRDLSKRLLPPTFGGETRQISVLKGLEALA
                     QQAAPQIVLIHDGARPFPSRDLIDRARDAAIARGAAVPGLGPTDTVKQIDADGRIVAT
                     PPRDGLRTVQTPQAFRFDLILSAHRKAAAAGKEGLTDDAAIAEWAGHPVYVFEGEQDN
                     MKITTADDIALAEAKLARTLDDVRMGQGYDVHAFGPGDHVWLGGLKIPHDHGLVGHSD
                     ADVLSHAITDAIFGAIGDGDIGSHFPPSDPQWRGAASSIFLAAAVEHLRKRGGMLAHV
                     DATLVCERPKIGPHRDAIRKSLAEIMGVSLDRVAVKATTSERLGFTGREEGMAALAIA
                     TVRLPL"
     gene            complement(1412..1651)
                     /locus_tag="VGB93_12405"
     CDS             complement(1412..1651)
                     /locus_tag="VGB93_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146918.1"
                     /translation="MMPDATNPGKAFDNAVRVTDENGKILATFTGDDLKAWRQFQLDA
                     VKNFNVVVTDSSMPVGISKTIRGPDKRNDKLIASG"
     gene            complement(1831..2151)
                     /locus_tag="VGB93_12410"
     CDS             complement(1831..2151)
                     /locus_tag="VGB93_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146919.1"
                     /translation="MVYYPVGIAATPRVTLSGLFGAVERQASRPDPKLNDVYADFTLT
                     DAQLNVLSPGAQLELTVNADDGTLLESGTFDVADVATLKTLAKAAAADLKLFCTPEED
                     ALSF"
     gene            complement(2161..2466)
                     /locus_tag="VGB93_12415"
     CDS             complement(2161..2466)
                     /locus_tag="VGB93_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146920.1"
                     /translation="MNVKLRTLIAAAMIVAAPSAGAAEGCAGSAFSPEQNPIYELTLL
                     AQKSSTPRGTTADLSISARAPGINYPVRGTALALPSKIGESDIDVYLKALSAGLIPT"
     gene            complement(2648..3685)
                     /gene="cobT"
                     /locus_tag="VGB93_12420"
     CDS             complement(2648..3685)
                     /gene="cobT"
                     /locus_tag="VGB93_12420"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY0146921.1"
                     /translation="MTVVSSKSPKSLADLRSLLKNLPPPAEDSGAAARARQAELTKPP
                     GSLGRLEEIAEFFARWQGREIPILDRPLVLVFAGNHGIVAQGVSAYPPSVTQAMVDNF
                     GAGGAAINQICKMFALDLKVVPLDLDSTTKDFTRAPAMSEADTAAYFARGMASVPADA
                     DLVIIGEMGIGNTTSAAAIYTALFGGAAAHWAGRGTGVDDAGLTRKIAAIEAACAFHS
                     VSLGDPFEVLRRLGGREISAMAGAITAARRASIPVLLDGYVACAAAAMLHAENPDAVA
                     HCLAGHQSVEGAHADVLARLGKKPLLDLGMRLGEASGAAVAAGLVRAALACHSGMATF
                     AEAQVAGKTES"
     gene            3868..>4209
                     /locus_tag="VGB93_12425"
     CDS             3868..>4209
                     /locus_tag="VGB93_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0146922.1"
                     /translation="MDATDRKILQIIQTDSSLSIAAIAAQVGLSQTPCWKRIQKLEAE
                     GIIRRRIALLDPAKLGLGMTVFVTIEVGEHAHDKLDHFATTISAMGEVLDIYRMAGDV
                     DYLLKVVVADTK"
BASE COUNT          863 a         1254 c         1255 g          837 t
ORIGIN      
        1 gtgcatgctt atactttagt caaagttcac gttatagaca tttgtgcaac gcggcaacag
       61 tgacttgggc gccaagccta agttttagac ctgattgact gcggcggcga aacgcgacag
      121 gaaaacggcc cgttctgccg gtttggaagc cgcccgtagc atgagtaaaa cgaccgatct
      181 tgtgattttg gtggtggcgg ccgggcgcgg gacgagggcg ggacgaggct tacccaaaca
      241 atatcgcccc ctcgctggca agcccttatt ggggcatacg ctttttacgc ttttgcaagc
      301 ggcgccggaa gctcgcgtac tcgcggccat tcatcccgac gatgttgagt tttacaagca
      361 gacggccgcg gatttgccgc gggatctttc caaacgcctg cttccaccga cgtttggtgg
      421 cgagacgcgg cagatcagcg ttctcaaggg tctcgaggcc ctggcgcaac aggccgcgcc
      481 gcagatcgtc ttgatccatg atggtgcccg gccctttcca agccgcgacc tgattgatcg
      541 ggcccgcgac gccgcgatcg cgcgcggcgc tgccgtgccc gggctcgggc ctaccgatac
      601 ggtcaaacaa atcgatgctg acggccgcat cgtcgcgacg cctccgcggg acggattgcg
      661 cacggtgcag acgccgcagg cgttccggtt cgatctcatt ctgtcggcgc accgcaaagc
      721 ggccgccgcc ggcaaagagg gcctgaccga cgacgcggcg atcgccgaat gggcgggtca
      781 tccggtttat gtatttgaag gtgagcagga caatatgaag attacgaccg cagacgatat
      841 cgctttggcc gaggccaaac tcgcgcgcac gcttgatgat gtgcgtatgg gccaaggtta
      901 tgacgtgcat gcgttcggtc ccggcgatca cgtctggctg ggcggtctca agatcccgca
      961 cgaccacggg ctcgtcggcc attcggacgc cgatgtgctc agccatgcga tcaccgatgc
     1021 gatcttcggc gcgatcggcg atggcgacat cgggagccac tttccgccgt ccgatccgca
     1081 atggcgtggc gcggcgtcat cgatctttct cgccgctgcc gtcgaacatc tacgcaagcg
     1141 cggcgggatg ctggcgcatg tggacgcgac ccttgtctgc gaacggccga agatcggtcc
     1201 gcatcgcgat gcgatccgca aaagcctcgc cgaaatcatg ggcgtttcgc tggaccgcgt
     1261 cgcggtgaag gcgacgacat ccgagcggct cggctttacc ggccgcgaag aaggaatggc
     1321 ggcgctcgcc atcgcgacgg tgcggttgcc gctttagagt gtcgacgaca gcgcgtgacg
     1381 agcgagacgt ataaggcggc agtcgaagca ttcaacccga tgctatgagc ttatcgttgc
     1441 gtttatctgg ccccctgatc gtcttggata tcccgacggg catggagctg tcggtcacaa
     1501 cgacattgaa gttcttcact gcatcgagtt gaaactgccg ccaagccttt aaatcgtcgc
     1561 ccgtaaatgt ggcgagtatc ttgccgttct cgtcggtgac gcgaaccgcg ttgtcgaagg
     1621 ctttgccggg gttcgttgca tcgggcatca tgcgatactc cggaataacc gaaaccattc
     1681 caaacttaca tcgatgatga cctgtccgca atcttgatgg cgaaaagtgc tatgcggccc
     1741 atgctatatc tagaagtata cagatgagcg aatgtttcac gctacatcgc acttgcagca
     1801 attgctgatg agtcgttcga ctcagcaaag ctaaaacgaa agcgcatctt cttcgggcgt
     1861 gcaaaacaat tttagatctg cggcagccgc tttcgccaac gtcttcagcg tcgccacatc
     1921 ggcgacgtcg aaggtgccac tttccaacaa ggttccgtcg tccgcattga ccgtcagctc
     1981 aagctgcgca cccggtgaaa gcacgttgag ttgtgcgtcg gtcagcgtga aatcggcata
     2041 gacgtcattg agctttgggt cggggcgcga tgcctggcgt tcgaccgcgc cgaataggcc
     2101 gctcagcgta acccgcggcg tggcagcgat cccgaccgga taatagacca tgacttttat
     2161 ctaggtcggt atcaggcctg cgctaagcgc tttgaggtac acgtcgatat cgctttcgcc
     2221 gatcttgctt gggagcgcca gcgctgtacc gcgtaccgga tagtttatgc ctggcgcacg
     2281 cgccgaaatc gacaaatccg cggtggtgcc acgtggggta ctcgacttct gagccaacag
     2341 agtcagctca tagatcgggt tttgctcagg ggagaaagct gagcctgcac agccctcggc
     2401 ggcgcctgcc gagggcgccg cgacaatcat cgccgccgct atgagggtcc tgagcttgac
     2461 gttcatacgt cccccggaag cgctgacatc ggctgagcgc aaccttaggc agccgcggcc
     2521 cagcgtcaag gcactgccct cttagcccaa tcgcatgacg gacttgtagc cgagcccaat
     2581 cgagctaaaa cactccctta ccaagggagt gttttatcca gccccgaagt atcgtcgtta
     2641 cggctactta gctttcagtt ttgcccgcaa cctgcgcttc ggcaaaggtg gccatgccgg
     2701 aatggcaggc gagtgcggcc cggacgaggc ccgccgcgac ggccgcgccg ctcgcttccc
     2761 caagccgcat gccgagatcg agcaaaggct ttttgcccag ccgcgctagg acgtctgcgt
     2821 gtgcgccttc gacggattga tgccccgcaa ggcagtgtgc gacggcatcc ggattctccg
     2881 cgtgcaacat ggcggctgcg gcacacgcga catagccatc gagcaaaacc ggaatagacg
     2941 cgcgccgggc cgctgtaatt gcgccggcca tcgccgaaat ctcgcggccg ccgagacggc
     3001 gcagcacttc gaacgggtcg ccaagagaga cggaatggaa agcacatgca gcctcaatgg
     3061 cagcgatctt gcgcgtcagg cccgcatcat ctacccccgt ccctcgccct gcccaatgcg
     3121 cggcagcccc gccaaagagc gcggtgtaga tcgccgcagc gctcgtggta ttgccgatgc
     3181 ccatttcgcc gataatgacg agatcggcgt cagctggcac gcttgccatg ccgcgtgcga
     3241 aataggccgc tgtgtccgcc tcgctcatcg ccggcgctcg cgtaaagtcc tttgtcgtcg
     3301 aatcaagatc gagtggcacg actttcaaat ccagcgcgaa catcttgcag atctgattga
     3361 tcgcggcgcc gcccgcgcca aaattgtcga ccatcgcttg cgttacggag ggcggatagg
     3421 ctgagacccc ctgcgccacg attccatgat tgccggcgaa gaccagcacc aaaggccggt
     3481 cgaggatggg gatctcgcgg ccctgccagc gcgcgaaaaa ctcggcgatc tcctcgagac
     3541 gaccgagcga acccggcggt ttggtcaatt ccgcctggcg agcccgcgcc gccgccccgc
     3601 tatcttcggc tgggggcgga agatttttca ataggctgcg gagatcggcg agtgatttag
     3661 gcgatttgga agaaactacg gtcataaaag cactcaaaca gggttgcgcc gccccgcgca
     3721 accccgagcg ttacgcacag gtccaacatg ccgcttccgt aaattcatta aaaacagtga
     3781 attaggtctt ggacataatc tattattttt atgtagtaag tggacttaag aagaattatt
     3841 ctgacagcat ctattggggg aatatttatg gacgcgaccg accgcaaaat tcttcaaatc
     3901 attcaaacgg actcaagctt atcaattgcc gccatcgcgg cgcaggtcgg attgtcgcaa
     3961 accccgtgct ggaagcggat tcagaagctg gaggcggaag gcatcatccg ccgacgaatt
     4021 gccctgctgg acccagcgaa acttggtctg gggatgacgg tctttgtgac aatcgaggtc
     4081 ggcgaacacg cgcacgacaa gctcgatcat tttgccacga ccatctcggc gatgggcgaa
     4141 gtgctcgaca tctatcgcat ggcaggagac gtcgactatc tgctgaaggt cgtcgtggcc
     4201 gacactaag
//
LOCUS       DASWOG010000605         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_362462,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000605 DASWOG010000000
VERSION     DASWOG010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362462"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..789
                     /locus_tag="VGB93_12430"
     CDS             322..789
                     /locus_tag="VGB93_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0146923.1"
                     /translation="MKKAPNPIDRHVGSRVRMRRVLLGMSQEKLGEALGLTFQQVQKY
                     EKGTNRIGASRLQQISRTLNVPPSFFFDGAPLPDEQTGENGATQFAVAEPSNSTFVID
                     FIASAEGLHLNKAFARIQDPKVRKRIIDLVTSLAGDDPPAPLAGKKVEEETKP"
     gene            956..2152
                     /gene="metK"
                     /locus_tag="VGB93_12435"
     CDS             956..2152
                     /gene="metK"
                     /locus_tag="VGB93_12435"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0146924.1"
                     /translation="MARQNYLFTSESVSEGHPDKVCDRISDEIVDLYFRESAKAGIDP
                     YEVRVACETLATTNRVVIAGETRGPKLSSDVIEHAARKAIKAIGYEQDGFHWEHCKFE
                     VLLHAQSVDIAQGVDAAGNKDEGAGDQGIMFGFACRETPELMPAPLYYSHKILEVLAK
                     ARHAGEGDAAKLGPDAKSQVTIQYADGKPVAATQIVLSTQHIDPHLSSEDVRAIVEPY
                     IRRTLPAQWISDQTIWHVNPTGKFVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSG
                     KDPTKVDRSAAYAARYLAKNIVAADLADRVTIQLAYAIGYSEPLSIYVDTHGTGRVAE
                     DKLEAVLPQVMRLSPRGIRDHLQLNKPIYARTSAYGHFGRAPEADGGFSWEKTDLADA
                     LRASFS"
     gene            2186..2875
                     /locus_tag="VGB93_12440"
     CDS             2186..2875
                     /locus_tag="VGB93_12440"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY0146925.1"
                     /translation="MTDEHPLATRLYGRRKGKKLSALQVNLRETLLPRLALDPAQPIM
                     SAPCLFPNAPRDIWLEIGFGGGEHLAAEAAAHPDEGFIGCEFFENGVVKVLTLIAHKD
                     LDNVRLYQGDAGAIVDALPQQSLAGAYLLYPDPWPKRRQRKRRFLSDTMLKRLARIMR
                     PGAEIRFATDIDDNSGWTLARVSESPDFEWRAQSARDWREPWPGWASTRYEAKALAAG
                     RAPVYLSFVRT"
     gene            complement(2931..3704)
                     /locus_tag="VGB93_12445"
     CDS             complement(2931..3704)
                     /locus_tag="VGB93_12445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0146926.1"
                     /translation="MPEARKNTPRTSDLAFIEVSHAGETFRIALKRISTARRFTLRVR
                     AATRDVVLTMPPRGSIIGARNFVERHAAWIGVRLQRLPAPMPFGAGEIVPVRGVNHRI
                     VERPHQRGTAWVEAASDPQSDKSLPMLCVAGERAHVARRVQDFLMREAKRDIETAVAR
                     HAGKLGVSPRRITLRDTTSRWGSCSSTGALNFSWRLIMAPSYVLDYLAAHEVAHLVHM
                     NHSPAFWKILGKLSAHVERAEAWLKINGAGLLRFGPFKA"
     gene            complement(3862..>4166)
                     /locus_tag="VGB93_12450"
     CDS             complement(3862..>4166)
                     /locus_tag="VGB93_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY0146927.1"
                     /translation="IETVFVRHLLPEGKMTHDNQFIDLSAINRTALMMVEGEKDDISG
                     LGQTKAAQDLCTNIPSKLKLHYEQDNVGHYGVFNGSRYRQFIAPKIREFHAAVAPV"
BASE COUNT          856 a         1270 c         1228 g          812 t
ORIGIN      
        1 ggccgccgag agcaccgcgc agatgcgcaa ggtcatcgtg agctttaagt ttgtttgcgt
       61 cgcttctcgg acgatcgatt gtttgacaac cgtggataac gcggtattat atgagaaaat
      121 ctgccgtggg ggcgagaacg aaacgataca ataagctcgc gacaaatcgc gacactgaca
      181 tcgcgtttct gtcgcgtctc caccatcacg agaaacccgc ccgcatatac cggcttcgtc
      241 atggccattt cggcaatatc aacattattt acgatacgcg ccgaacccgt accccgcagc
      301 agtaaaggga aaaggtgact tgtgaagaag gctccgaatc cgattgatcg tcatgtcggc
      361 agccgggttc gtatgcgccg tgtccttctc ggaatgagcc aggaaaaact tggcgaagcg
      421 ttgggattaa cgtttcagca agtccagaaa tacgaaaaag gcacgaaccg tattggtgcc
      481 agccgtctgc aacagatttc gcgtacgctg aatgtcccgc cgtcgttctt ttttgacggc
      541 gcgcctctgc cagacgaaca gacaggggaa aacggcgcca cgcaattcgc tgtcgccgag
      601 ccttcaaact cgactttcgt gatcgacttc atcgcctcgg cagagggcct gcatctcaac
      661 aaggctttcg cgcgcattca ggacccgaaa gtacgcaaac ggatcatcga tctcgttacg
      721 agcctcgcgg gtgatgatcc cccggcaccg cttgcgggta aaaaagtcga agaagagacg
      781 aagccttagg ccagatggcc agggccacca ttcgttataa cgaacgcttc ctgccgaaaa
      841 gcttgaccta acgaacggat tggctgaaag ttggcccaat cgcttggcct gtgtctccca
      901 ctgtggccga gaggatttca cccttttgag gcactcggcg actctggagt atttcgtggc
      961 tcggcaaaat tatctgttca ctagcgaatc cgtctctgaa ggccatcccg ataaagtctg
     1021 cgaccggatc tccgacgaaa tcgtcgatct ttacttccgt gaatccgcca aagccggcat
     1081 cgatccttac gaagtccgcg tcgcctgcga gaccttggcg acgaccaatc gtgtcgttat
     1141 tgccggcgag acgcgcgggc caaaactctc ctccgatgtc atcgaacatg cggcacgcaa
     1201 ggcgattaaa gcgatcggct atgagcagga cggcttccat tgggagcatt gcaaattcga
     1261 ggtcctgctg cacgcccaat ccgtcgatat cgcgcaaggt gtcgatgccg caggcaacaa
     1321 ggatgaaggc gccggcgacc agggcattat gttcggcttt gcctgccgcg aaacgccgga
     1381 gctgatgcct gcgccgcttt attattcgca caaaattctt gaggtcctcg ccaaggcccg
     1441 tcacgcgggc gagggcgacg ccgccaagct tggccccgac gccaagagcc aggtgacgat
     1501 ccaatatgcc gacggcaagc ctgtcgcggc gacgcagatc gtgctctcga cgcagcatat
     1561 tgatccgcat ctgtcgtcag aagacgtgcg cgcgattgtc gagccttaca tccgcaggac
     1621 gctaccggcg cagtggatca gcgaccagac gatctggcac gtcaacccga ccggcaagtt
     1681 cgtcatcggc ggccctgacg gcgataccgg cctcaccggc cgcaagatca tcgtcgatac
     1741 gtatggcggc gcggccccgc acggcggcgg cgccttctcc ggcaaggatc cgaccaaggt
     1801 cgatcgctcg gcggcctatg cggcgcgcta cctcgccaag aacatcgttg ctgccgatct
     1861 cgccgaccgg gtcacgattc agcttgccta tgcgatcggc tattccgagc cgctgtcgat
     1921 ttacgtcgat acgcatggca ccggccgagt cgcggaagat aagcttgaag ccgtgctgcc
     1981 gcaggtgatg cgtctctcgc cccgcggtat ccgcgaccat ctgcaattga acaagccgat
     2041 ctacgcgcgc acctcggcct atggccattt cggccgggca cccgaggccg acggcggttt
     2101 ctcgtgggag aagacggacc tcgccgacgc gctccgcgcg agcttcagct aaacgacatt
     2161 ccgacaaaca cacgcgatct cgcgcatgac cgacgaacac ccccttgcga cgcgccttta
     2221 tgggcgccgc aagggcaaaa agctgagcgc cctgcaggtc aatctgcgcg agactttgtt
     2281 gccgcggctg gcgcttgatc cggcgcagcc gatcatgtcc gcaccttgtt tgtttccaaa
     2341 cgcgccgcgc gacatctggc tcgaaattgg ttttggcggc ggcgaacatc tggccgctga
     2401 agccgcggcg catcccgacg agggtttcat cggctgcgaa tttttcgaaa atggcgtcgt
     2461 caaggttttg actctgatcg ctcacaaaga ccttgataac gtccgcctct atcaaggcga
     2521 cgctggcgcc atcgtcgatg cgctgccgca gcagagcctc gccggcgcat atttgcttta
     2581 tcccgaccct tggccgaagc ggcgtcagcg caaacgccgt tttctttccg acacgatgct
     2641 gaaacgtctc gcgcgcatca tgcgccccgg cgcggaaatc cgcttcgcca ccgacatcga
     2701 cgacaattcc ggctggacgc tggcgcgcgt gtcggaatcg cctgatttcg agtggcgcgc
     2761 gcaatcggcg cgcgattggc gtgaaccgtg gccgggttgg gcttcgactc gctacgaggc
     2821 aaaggcgctc gcagccgggc gcgcaccggt ctatctcagc ttcgtgcgga catagagcgt
     2881 tttcgagtga agtagtgacc ggttcgcgcg aagaaaacgc gttaaaaaaa tcaggccttg
     2941 aaaggcccga agcgcagcaa tcccgcacca ttgatcttga gccaggcctc cgcgcgctcg
     3001 acatgcgcgg aaagcttgcc taggattttc cagaacgccg gcgaatgatt catgtgaacg
     3061 aggtgcgcga cttcatgggc cgcgaggtag tcgagcacat agctcggcgc cataatcagt
     3121 cgccaggaga aattgagcgc accggtcgag gagcatgagc cccagcggct ggttgtgtcg
     3181 cgcagagtaa tcctgcgcgg gctgacgccg agcttgcctg cgtggcgcgc gacggcagtc
     3241 tcgatgtcgc gtttggcctc gcgcatcaga aaatcctgca cacgccgcgc tacatgcgcg
     3301 cgctcgcccg ccacgcacag catcgggaga ctcttgtcgc tttgcggatc tgacgccgcc
     3361 tcgacccagg cggtgcctcg ctgatgcgga cgttcgacaa tgcggtgatt gacgccacgg
     3421 accggcacga tctcaccggc gccgaatggc ataggcgcag gcaatcgttg caggcgcacg
     3481 ccgatccacg cagcatgcct ctcaacgaaa ttgcgcgcgc cgatgatcga gccgcgcggc
     3541 ggcatggtaa gcaccacatc acgcgtcgcg gcgcgcacgc gcagcgtgaa acgtcgagcg
     3601 gtgctgatgc gcttcaacgc aatccgaaac gtttcgccgg cgtgcgaaac ctcgataaag
     3661 gcgaggtcag atgttctcgg tgtgttctta cgagcttccg gcatgggccg aatttagccg
     3721 cagcccgcct gagtcgctac tgaatcaggg gctcacattg gcgacatttt accttggccg
     3781 cctcgggtcg cagtcttttt cgcggcgcag catagagggc cgtcacgccg ccacaggcca
     3841 atttaccgtg attgcaagag cttagaccgg cgcgacggcg gcgtggaact cgcggatttt
     3901 gggcgcgatg aactgccggt agcgcgaccc gttaaagaca ccatagtgcc cgacattgtc
     3961 ctgttcatag tgaagcttaa gcttggacgg aatattggtg cagaggtcct gtgcggcctt
     4021 ggtttggcca aggccggaaa tatcgtcctt ctcgccctcg accatcatca gggccgtccg
     4081 gttgatggcg gagagatcga tgaactgatt gtcgtgggtc atcttgccct ccggcaggag
     4141 gtggcgcacg aaaaccgtct cgatcg
//
LOCUS       DASWOG010000606         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_143606,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000606 DASWOG010000000
VERSION     DASWOG010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143606"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..795
                     /locus_tag="VGB93_12455"
     CDS             10..795
                     /locus_tag="VGB93_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0146928.1"
                     /translation="MHARAPEDSAGFAVLTLLALFADIHGNREALDACLADAQRRGAE
                     RLIFLGDMIGYGADPAYVVDVVAGRLRDGAMAVLGNHDAALIAGCDSMNSVAAASIEW
                     TRRRLDKNQQDFLAGLPLTAERGDLLFVHAEAAQPRFWTYVTTSAEARRSLRATSKRV
                     TFCGHVHRPQLYAAATFGLPNARAPKASVPISVAGSGKWLAVLGSVGQPRDENPAAAY
                     AIYDDTDGRLTFLRVAYDISTAAKKIRAAGLPDILAARLFVGR"
     gene            884..1105
                     /locus_tag="VGB93_12460"
     CDS             884..1105
                     /locus_tag="VGB93_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEY0146929.1"
                     /translation="MNRYERPPLSAGEAEIEYLDGDIRVLRPGTFVRCAATGVPIPLE
                     ELRYWNVDLQEPYASYHAKLQRMGMKLAK"
     gene            complement(1130..2446)
                     /locus_tag="VGB93_12465"
     CDS             complement(1130..2446)
                     /locus_tag="VGB93_12465"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY0146930.1"
                     /translation="MIKTLSILPIYQAASAAFAPLSPLVLYCRRKLRHDDYDRDGERL
                     GRPSEPRPPGPLAWLHGASVGESLALLPLVERLAARGLTILVSTGTSAAAAVIVPRLP
                     AGALHQFLPLDVPQFLARFLDYWRPDLALIADSEIWPNLFLATRRRKIPLLLINARIS
                     QSSFQRWRWFPRFIATVLANVDLCLAQSEADAERFALLGVRHVKVAGNLKYDVNAPPA
                     DRQSLAALAVRIGSRPVWVAASTHRGEEEIVLAAHQAMEQTIPGLLTIIIPRHARRGA
                     EIAGLAAARGFAVAQRSSEFEVDENALPDIYVADTNGELGLFYRLTGIALLGKSLLPS
                     LSGGQNPIEAAKLGCAILHGPNVANFEPIYRALDDARGAVPVADADTIARVVAILLAD
                     AGRLRLMGRAASETVKSLGGACDRIMSAIEPHIAQLSIESRQQPMN"
BASE COUNT          531 a          821 c          796 g          462 t
ORIGIN      
        1 gcccgacaaa tccacgcgcg ggcgcccgaa gacagcgcag ggttcgctgt tttgaccttg
       61 ctcgccctct ttgccgacat tcacggcaat cgcgaagcgc ttgacgcctg cctagccgac
      121 gcacagcgcc ggggcgcaga acggctgatt tttctggggg atatgatcgg ctatggcgcc
      181 gatccagcct atgtcgtcga tgtcgtcgcc ggacggctac gcgatggcgc catggctgtc
      241 ctcggcaatc acgacgccgc gctgatcgcc ggctgcgaca gtatgaacag cgtcgcggcc
      301 gcatcgatcg aatggacgcg ccggcggctc gacaaaaacc agcaggattt cctcgccggg
      361 ctgcccctca ccgccgaacg cggcgacctc ctctttgtcc atgccgaagc cgcccaaccg
      421 cggttctgga cgtatgtgac aacttccgcg gaggcgcgtc gctcattgcg cgccacttcg
      481 aagcgcgtga ccttctgcgg ccacgttcac cgcccgcagc tctatgctgc cgccactttc
      541 ggcctcccga acgctagggc gccaaaggcc agtgtcccga tttcggttgc gggatcaggc
      601 aaatggctgg ccgtgctcgg ctccgtcggc cagccgcgcg atgaaaaccc tgccgcggcc
      661 tatgccattt acgacgacac ggacggacgg ctgacctttc tgcgggtcgc ctacgacatc
      721 tcgactgccg ctaagaaaat ccgtgccgcc ggcctgcccg acattctggc ggcccgcctt
      781 ttcgtcgggc ggtagacaaa atccgtcctg cggtggcgcc tgtggaaatt cccccatagc
      841 tgcgctatct tcaataaaac cctcttatca gccacgtccg agaatgaacc gctacgaacg
      901 tccaccacta tccgccggcg aagccgagat tgaatatctc gacggcgata ttcgtgtttt
      961 gcggcctggc actttcgtcc gctgcgcggc caccggcgtt ccgattccgc tcgaagaatt
     1021 gcgctattgg aatgtcgatc tgcaggagcc ttacgcgagc tatcacgcca aattgcagcg
     1081 catggggatg aaactcgcga agtgatttcg cgcgataatt aaaccgcgat cagttcattg
     1141 gctgttgccg gctttcaatc gagagttggg cgatgtgcgg ctcgatcgca ctcataatgc
     1201 ggtcgcaggc accgcccagg ctcttcaccg tctcgctcgc agcgcgcccc atcagtctca
     1261 atcggccggc gtcggcaaga aggatcgcga cgacgcgcgc gatcgtgtcg gcatcggcaa
     1321 caggcacggc gccgcgcgca tcgtcgagcg cacgataaat cggctcgaaa ttggcgacgt
     1381 ttggtccatg caagatggcg cagccgagct tggcggcttc gatgggattt tggccgccgc
     1441 tcagggatgg caggagcgac ttgcccaaaa gcgcgatgcc ggtcaggcga tagaacaatc
     1501 caagctcgcc attcgtgtcg gcgacgtaga tatccggcaa cgcgttttca tcgacttcga
     1561 attcggacga tcgctgcgcg accgcgaagc cgcgcgcggc agcgaggccg gcgatctccg
     1621 caccgcgccg cgcatgacgc ggaatgatga tggtcaataa accggggatc gtctgctcca
     1681 tcgcctgatg cgcagcgagc acgatctcct cctcaccgcg atgggtggac gctgcgaccc
     1741 aaaccggccg cgaaccaatg cgcacggcga gcgccgcaag gctctgccgg tcggctggcg
     1801 gcgcattgac atcgtatttg agatttccgg cgactttgac atgccgcaca ccaaggaggg
     1861 caaaacgctc cgcgtcggct tcgctttgcg ccaggcataa atcgacattt gcgagcacgg
     1921 tcgcaatgaa gcgcggaaac catcgccagc gctgaaacga gctttgcgaa attcgcgcat
     1981 tgataagcag cagcgggatt ttacggcgcc gggttgccag aaagagattg ggccagatct
     2041 ccgagtcggc gatgagcgcg agatcgggac gccaataatc tagaaatcgc gcgagaaatt
     2101 ggggtacgtc gagcggcaga aattgatgta acgcaccggc cggcaggcgc ggcacgatga
     2161 ccgccgcggc agcactcgtg ccggtcgaaa ccagaatcgt aagcccgcgt gcggcaagcc
     2221 gttcgaccag aggcagcaag gcaaggcttt cgccgacgct tgcgccatgc agccacgcca
     2281 aagggcctgg tggccgcggc tcgctcgggc gtcccagccg ttcgccatcg cgatcgtaat
     2341 cgtcgtgccg caattttcgc cggcaataaa gcaccaacgg gctcagcggc gcaaaggcag
     2401 cgctggcggc ctgataaatg ggcaaaatgg aaagggtttt gatcaagacg caaacgactg
     2461 ccaagatgaa ccaagcggcg acgccggcgg cccgtaatga acaggcagag cgcaccgcaa
     2521 tcaagtgccc ggaggcaaca acgcatgttt gacagacgtt tgcggccaga gcttgcggag
     2581 gtgcgaaatt gatcgcgaaa aagcgatttc
//
LOCUS       DASWOG010000607         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_107224,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000607 DASWOG010000000
VERSION     DASWOG010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107224"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..898)
                     /gene="murI"
                     /locus_tag="VGB93_12470"
     CDS             complement(62..898)
                     /gene="murI"
                     /locus_tag="VGB93_12470"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0146931.1"
                     /translation="MSKPPHVLIFDSGLGGLTVLAEVMRLRPDVSCLFAADDAGFPYG
                     RLAEPALIARVGTVVAHLISDYAPDAVIIAWNTATTTVLPHLRASYPQIPFIGTVPAV
                     KPAAAQSRSKLISVLATPGTVARDYTHDLVRTYAAHCDVTLVGSTQLASLAEACMKGE
                     DVADAAMVAEIAPSCVEKGGQRTDCIVLACTHYPLLLKQFERLASWPVAWIDPAEAIA
                     RRLDHVLQEKLGVAAPDTENWPQNAAMFTSGAVPSPALAKALHERGIGGILENPIPMA
                     IA"
     gene            1070..>1504
                     /locus_tag="VGB93_12475"
     CDS             1070..>1504
                     /locus_tag="VGB93_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0146932.1"
                     /translation="MATEKASNDESIIETRVPARLDRLPFGAFHLRVIVALGITWILD
                     GLEVTLAGSLAGALVASPTLHFNDWDIGLISGFYLAGAVVGALIFGWLTDRYGRKKLF
                     FITLGLYALATAATAFSFNLASFCLFRFLTGAGIGGEYAAINS"
BASE COUNT          278 a          450 c          473 g          303 t
ORIGIN      
        1 ctctcgttgc gcagatgcgt cgcgacgagg ccgaggcgcg aaaaattctc gcggctgatg
       61 cctaagctat cgccatcggg atcgggtttt cgagtatccc gccgattccc cgctcatgaa
      121 gcgctttggc aagcgccgga cttggcacgg caccgctggt gaacattgct gcgttttgcg
      181 gccaattctc tgtgtcgggc gcggcgacgc cgagcttctc ctgcagcaca tggtcgagcc
      241 ggcgtgcgat ggcctcagcc gggtcgatcc aggcgacggg ccaggacgca agccgctcga
      301 attgcttgag cagcagcgga taatgggtgc aggcgagcac gatacaatcg gtgcgctggc
      361 cgcctttttc gacacagctc ggcgcgattt ctgcaaccat cgcagcgtcg gcgacgtctt
      421 cacccttcat gcaagcctcg gcgaggcttg caagttgcgt cgagccgacg agcgtcacgt
      481 cgcaatgggc ggcataggtg cgcaccaggt cgtgagtata gtcccgtgcg actgtgccgg
      541 gtgtcgcaag aacggaaata agtttcgagc gcgattgtgc cgccgccggc tttactgccg
      601 ggaccgtgcc gatgaaggga atctgcggat aagaggcgcg tagatgcggc aacaccgtag
      661 tcgtcgcagt attccaggcg atgatgacgg catcgggcgc ataatccgaa atgaggtgag
      721 ccaccaccgt gccgacgcgc gcgattaacg cgggttcggc cagccggccg tagggaaaac
      781 ccgcatcgtc cgccgcgaaa aggcagctca cgtccggccg caggcgcatc acttccgcca
      841 gaacggtcaa gccgcccaag ccagaatcaa agatgagaac gtgcggcggt ttcgacaatg
      901 agcctccggg caagcgatgc gtaattataa cccggtcctt agctctttcg cgcgccaaga
      961 tcgacccatt tcgagggacg agaggcgcgg atgcaacagt tcgcgccgac tgcgtttcca
     1021 tttgagcctt tttgacgcgc cgcgcgttgt ggcccgagca gaggggttca tggcgaccga
     1081 aaaagcatcc aatgacgaat caatcattga gacgcgcgtc cccgcccgtc tcgaccggct
     1141 gccctttggc gccttccacc tgcgggtgat tgtcgcgcta ggcatcacat ggattctcga
     1201 tgggcttgag gtgacgctgg ccggctcgct cgccggagcg ctggtggcaa gcccgacgtt
     1261 gcatttcaac gattgggaca tcggcttaat ttccggcttc taccttgctg gtgcggttgt
     1321 cggggcgctc attttcggct ggctcaccga ccgctacggg cgcaagaagc tcttcttcat
     1381 cacgctcggc ctgtatgcat tggcgacagc ggcgacagcc tttagcttca atctcgcgag
     1441 cttctgcctg tttcgttttt tgacaggcgc tgggattggc ggcgagtacg ccgcgatcaa
     1501 ttca
//
LOCUS       DASWOG010000608         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_33502,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000608 DASWOG010000000
VERSION     DASWOG010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33502"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGB93_12480"
     CDS             complement(<1..269)
                     /locus_tag="VGB93_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY0146933.1"
                     /translation="MRRLLLASAFLVAGFPALAADLLPYSPAPYYEPAPIFTWTGAYV
                     GASAAFGFGRFTHDGGPYFGDASGGLFGIQAGYNYQSGPLVAGIEG"
     gene            507..1265
                     /locus_tag="VGB93_12485"
     CDS             507..1265
                     /locus_tag="VGB93_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0146934.1"
                     /translation="MARKAALVTGAARRIGLAIAERLAGAGYDLAIHASDATRPEADE
                     AAARLQAQGHKAVVIAAELSDPAQPVRIVEAAKAALGPLSVLVNNASIFEHDSADAID
                     IVLFDKLMAVNLRAPLLLAQHFANQVPDGASAVIVNLVDQRVWRPTPQFFSYSVSKAG
                     LWWATQTMAQAFAPRRIRVNAIGPGPVLPNTPQGDAGFEKEVAGVLLKRAVAPAEIAD
                     AVLYLVEAQSVTGQMIAVDSGQHLAWQTPDIPEV"
     gene            complement(1278..1553)
                     /locus_tag="VGB93_12490"
     CDS             complement(1278..1553)
                     /locus_tag="VGB93_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146935.1"
                     /translation="MSAGRKTVLGKSPLGKTPSEIARRLGKRPKRESPGAWRRETFTL
                     PRVEARDKAREWFSAFPKAAYMTEIEFWRELDDGKIEFTIRRLPSAD"
     gene            complement(1601..2131)
                     /gene="thpR"
                     /locus_tag="VGB93_12495"
     CDS             complement(1601..2131)
                     /gene="thpR"
                     /locus_tag="VGB93_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY0146936.1"
                     /translation="MPRLFTGLEIPSDLARDLGMLRGGISGARWIDTEFYHLTLRFIG
                     DIDYATARDVCGTLEQIRRPSFTVTLESLDSFGGGKPRAIVAKAKPAAPLVELQAEQE
                     RLMRRLGIPPEPRKFTPHVTLARLRDASSTAVAAYLATRGFFSRRFEATRFVLFSARA
                     STGGGPYLVEAEYPLG"
     gene            complement(2292..2933)
                     /locus_tag="VGB93_12500"
     CDS             complement(2292..2933)
                     /locus_tag="VGB93_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0146937.1"
                     /translation="MRVPFLMGLALSIPAVFGLGLAPAAAKTIKLVAFGDSLTAGYLL
                     PADAAFPVVLEKALRDAGYDVRIANAGVSGDTAEDGLARLDWALQDGADGVILELGAN
                     DMLRGLDPARTRGTLDTIIAKLAAQHIKVLMAGMLASPSLGVEYGRTFDAIYPSLAAK
                     YKVPLYPFFLQGIAGNKSLQLNDGLHPNRDGVRKIVAQMLPLTENFVRAIAVQ"
     gene            2944..3657
                     /locus_tag="VGB93_12505"
     CDS             2944..3657
                     /locus_tag="VGB93_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0146938.1"
                     /translation="MMKDNAAIIFDNVDLSLGRGAARVHILKSISLTIARGEAVGLTG
                     PSGSGKSTLLMAMAGLERPDSGSVIVDGVTLDGLSEDALARFRGARIGIVFQSFHLIP
                     TMTALENVAVPLELAGEARAQARARDELAAVGLAARFDHYPAELSGGEQQRVALARAL
                     APKPSILIADEPTGNLDEENGTLVVDLLFALQRARGATLVFVTHDQSRAKRCDRVVRL
                     RSGRVMAAGPELEKATSQT"
BASE COUNT          782 a         1087 c         1161 g          706 t
ORIGIN      
        1 ccctcaatac cggcgacgag cgggccggat tggtaattgt aacccgcctg aatgccgaag
       61 agaccgccgg atgcatcgcc gaaatagggg ccgccatcat gcgtgaatcg gccgaagccg
      121 aaggctgcac tggcgccgac ataggcgccc gtccaggtga agatcggtgc cggctcataa
      181 taaggcgccg gcgaataggg gagcagatcg gcggccagcg ccggaaagcc agccaccaaa
      241 aatgcgctcg cgagaagcag ccgtctcatc tctcacctct tcacacgaaa actcttggcg
      301 aagaggatta ggatgggctg gttacctaaa acttaaataa agacttcgta aaaaacaaaa
      361 atatttgaat aataagtcat taaccatgcc tataaacgtc tatgaatact tatgattaac
      421 agcgattaag atgtttgcgc cggaacctct cgtcccgcgc gccgaaattg cctaaacagg
      481 cgcgggcatc aaatcgggga ggtatcatgg cgcgcaaggc tgctctcgta acgggcgctg
      541 cccgcagaat tggacttgcg attgccgaac gtcttgcagg cgcgggctat gatctcgcca
      601 tccatgccag cgacgcgacg cggccagagg ccgacgaagc ggctgcgcgt ttgcaggcgc
      661 aaggacataa ggcggtcgtc atcgctgctg agctgagcga tccggcacag ccggtgcgca
      721 tcgtcgaagc ggccaaagcc gcgctcggtc cgctcagcgt tctcgtcaac aatgcttcga
      781 tcttcgagca tgattccgct gacgcgatcg acatagtcct gttcgacaag ctcatggcgg
      841 tgaatctgcg tgcgcccttg ctgctggcgc agcatttcgc caatcaggtg ccggatgggg
      901 cgtccgcggt gatcgtcaat ctcgttgatc agcgggtttg gcggccgacg ccgcaatttt
      961 tctcctacag tgtcagcaag gccgggcttt ggtgggcaac gcagacgatg gcgcaggcct
     1021 tcgcgccacg ccgcatccgc gtcaacgcga tcggaccggg accggtgttg ccgaatacgc
     1081 cacagggcga tgccggtttc gagaaagagg tcgccggcgt gctgctcaaa cgcgccgtgg
     1141 cgccggcgga aatcgccgat gccgtgctct accttgtcga agcgcaaagc gtgaccggcc
     1201 agatgatcgc cgttgactcg ggccagcatc tcgcctggca gacgccggat attccggaag
     1261 tatagggacg ttgaaaccta atctgcgctg ggcaggcggc ggatggtgaa ctcgatcttt
     1321 ccgtcgtcaa gttcgcgcca gaactcgatt tcggtcatgt aggcggcttt cggaaaggcg
     1381 ctgaaccatt cgcgggcttt gtcgcgcgct tcgacacgcg gcagcgtgaa ggtctcgcgc
     1441 cgccaggcgc cgggtgactc gcgcttaggc cgcttgccga gccggcgggc gatttcggaa
     1501 ggtgtcttgc ctaaaggtga cttgcccaaa actgtcttgc gtcccgcact catgtcgcgg
     1561 ctcccgcaga ttagataaga cgctttctgg cgttcgccag ttacccgaga ggatactcag
     1621 cctcgacaag ataagggccg ccgccggtcg acgcccgtgc ggagaatagg acaaaccgcg
     1681 tcgcctcgaa gcggcgggaa aagaacccgc gcgtcgcaag atatgcggcg accgcggtcg
     1741 aggaggcatc gcgcagccgc gcgagtgtca catgcggcgt gaacttgcgt ggctcgggcg
     1801 gaatgccgag acgtcgcatc aggcgttcct gctcggcttg aagttcgaca agcggcgcgg
     1861 ctggctttgc cttggcaaca atggcgcgtg gtttgccgcc gccaaaggaa tcgaggcttt
     1921 cgagagtcac cgtgaaagac ggacggcgga tttgctcaag cgtgccgcag acgtcccgcg
     1981 cggtcgcata atcgatgtcg ccgatgaagc gtaaggtcag atgatagaat tcggtgtcga
     2041 tccagcgagc gccggatata cctccgcgca gcatcccgag atctctggca aggtcagaag
     2101 gaatctcgag gcccgtgaac aaacggggca tgggatcctc ctctcgtgac gacccaagcg
     2161 cgcagggcgc gagacggtct gatcatcttg cgagagggcc cgcgcaacgt cttgaggacg
     2221 aagcccgaat cgatctcacc tgtttattgt ggaccggaat cctcttttga gaaagatcgg
     2281 gtcagcgaaa tttattgcac cgcaatcgcg cgcacaaaat tctcggtaag tggcagcatt
     2341 tgcgcaacaa tcttccggac gccgtcgcgg ttcggatgaa gcccgtcatt gagttgcagg
     2401 cttttatttc cggcgatgcc ctgcaagaag aacggataga gcggcacttt gtattttgcc
     2461 gcgagcgacg gatagatggc atcgaacgtg cgcccatatt caacgccaag gctgggcgag
     2521 gccaacatac cggccatcag cacttttatg tgctgggcgg cgagcttggc gatgatcgta
     2581 tcgagtgttc cgcgtgtgcg ggcagggtca agaccgcgca gcatatcgtt ggcgccgagt
     2641 tcgaggatga cgccatcggc gccgtcctgt agcgcccaat caagccgcgc gaggccgtct
     2701 tcggcggtgt cgccggaaac gccggcattc gcgatgcgga catcatagcc cgcgtcgcgc
     2761 agcgcttttt cgagcaccac cgggaaagcc gcgtcggctg gcaacaaata cccggctgtg
     2821 agactgtcgc cgaacgcaac gagcttgatc gtcttggcgg ccgccggcgc gaggccaagc
     2881 ccgaaaaccg ccggaatcga aagtgcaagt cccatcagga acggcacacg caatctggat
     2941 gcaatgatga aagataatgc ggcgatcata ttcgataatg tcgatttgag cctcggccgc
     3001 ggcgcggcgc gtgtccatat cctgaaaagc atctccttga ccatcgcgcg cggcgaagcc
     3061 gtgggtctca cggggccatc cggttcaggc aaatccacgc tgctgatggc gatggccggg
     3121 cttgagcgcc cggattccgg cagcgtgatc gtcgatggcg tcactctcga tggactcagt
     3181 gaggatgcgc tggcgcgatt tcgtggcgcg cggatcggca ttgttttcca gtcttttcat
     3241 ctgatcccga caatgactgc gctggagaac gtcgccgtgc cgctcgaact cgccggagag
     3301 gcacgagccc aagcgcgcgc ccgcgatgaa ctcgcggcgg tgggcctcgc cgcacgcttt
     3361 gaccattatc cggccgaact ctccggcggc gaacaacaac gcgtcgcact ggcgcgtgcc
     3421 ttggcgccca aaccgtcgat cctcattgcc gatgagccga ctggcaatct tgacgaagaa
     3481 aacgggacgc tcgtcgtaga ccttttgttc gccttgcagc gtgcgcgtgg cgcgacgctc
     3541 gtctttgtca cgcatgatca aagtcgggcc aagcgctgtg atcgcgtcgt gcgtttgcgg
     3601 tcgggccgcg taatggcggc cgggccggaa ttggaaaagg ccacatctca aacgtagtaa
     3661 acgatcggta aatgcacctt tacctaaaaa ctaaccgaaa aattacacat tggggcgcac
     3721 aagtacacat tgttac
//
LOCUS       DASWOG010000609         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_216086,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000609 DASWOG010000000
VERSION     DASWOG010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216086"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VGB93_12510"
     CDS             <1..356
                     /locus_tag="VGB93_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY0146939.1"
                     /translation="TPKIEGGSDGTFVADARASLDDVSQALDRDLSDLVDAEDVDTLG
                     GLVSSLAGHVPLRGETLTADGFQFEVLDADPRRVKRIKIRRIMPEQPAAPESRSPANE
                     AEAPQGGLSEAAKSR"
     gene            410..>1518
                     /gene="lnt"
                     /locus_tag="VGB93_12515"
     CDS             410..>1518
                     /gene="lnt"
                     /locus_tag="VGB93_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0146940.1"
                     /translation="MFSAAEFVILAGGWRRRLIGFCGGACGALALAPVDFLVAMIVPM
                     TIAVWLIDGAAEAKQAPGAKLRSAAVRNAFTAGWWWGFGYFVAGFWWLGAAFLVEPQD
                     FVWAMPLGIFGLPAFLALFPALGFALARLGWSAGPSRILMLAAALGFSEWLREHVLTG
                     FPWNDYGMALGDHLLLAQFAAIGGLPALNVLTVAIFAAPALLADAPRKGGSRLPAGLV
                     VAIGAFLGLCVFGALRLAAAPPRELPGVKVRLIQPNIAEDENFTAAHKDEIVAHYLAL
                     SDRATSPEHTGLADVSLLIWPESAFPFILTEDPQALATIGNALPPNATLVTGAARIGI
                     VSRADKEYPAYFNSMLAIAHGGIILDTYDKVHLVP"
BASE COUNT          245 a          462 c          475 g          336 t
ORIGIN      
        1 aaacgcccaa gatcgaaggc gggtccgacg gcacgttcgt tgccgacgcg cgcgcaagcc
       61 tcgacgatgt ctcgcaggcg ctcgaccgcg atctctccga cctcgtcgac gccgaagacg
      121 tcgatacttt aggcgggctg gtctcgagcc tcgcggggca tgtgccactg cgcggcgaga
      181 ctctcaccgc cgatggtttt cagttcgaag ttctcgatgc tgatccgcgc cgtgtgaagc
      241 gcatcaagat tcgacggatc atgcccgagc aaccggccgc gcccgaatct cgcagccctg
      301 cgaacgaggc ggaggcaccg caaggcggcc ttagcgaggc ggcaaaaagc cgatagattg
      361 catcgattcg ccaatacgcc ttccgcagat tttcagctcc gcgagacaca tgttttcagc
      421 cgccgaattt gtcatccttg ccggcggctg gcgacgccgc ttgattggct tctgtggcgg
      481 ggcgtgcggc gcgttggcgc tcgcacctgt cgattttctg gtggcgatga ttgtgccgat
      541 gacgatcgcc gtatggctca tcgatggcgc ggcagaagcg aagcaagcgc ccggcgcaaa
      601 gttgcgttcg gccgccgtgc gcaatgcgtt cactgcgggc tggtggtggg gctttggcta
      661 tttcgtcgcc ggcttctggt ggctcggcgc ggcttttctg gtcgagccgc aagatttcgt
      721 ctgggcgatg ccgctcggta ttttcgggct gcccgcgttt ctcgcacttt ttccggcatt
      781 gggatttgca cttgcgcggc ttggctggtc ggcgggaccg agccgcattc tcatgcttgc
      841 agccgcgctt ggcttcagcg agtggctgcg cgagcatgtt ctcacaggct ttccgtggaa
      901 cgattatggg atggcgcttg gcgaccatct cctgctcgcg caatttgcag ccatcggcgg
      961 attgccggcg ctgaacgtcc ttacggtggc gatctttgcg gcgccggcgc tcttggcgga
     1021 tgcaccgcgg aaaggagggt cgcgccttcc ggctggcctt gttgtcgcca tcggcgcatt
     1081 tctcggcctc tgcgttttcg gcgctttgcg tctcgcggct gcaccgccgc gcgaactgcc
     1141 gggcgtgaaa gttcgcctga tacagcctaa tatcgcggag gacgaaaact tcaccgctgc
     1201 acataaggat gagatcgttg cgcattatct cgcactatcc gatcgcgcga cgagcccgga
     1261 acatacaggt cttgctgatg tcagcctgtt gatctggccg gagtccgcct ttccctttat
     1321 cctgacggaa gacccgcagg cacttgccac gataggcaat gcgttgccac caaatgcaac
     1381 gctcgtcaca ggggcggcgc gtatcggcat cgtcagccgc gccgacaagg aatatccggc
     1441 ctatttcaat tccatgctgg cgattgcaca cggcggcatc attctcgaca cttatgataa
     1501 agtgcatctg gtgccgtt
//
LOCUS       DASWOG010000610         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_327185,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000610 DASWOG010000000
VERSION     DASWOG010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327185"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VGB93_12520"
     CDS             <1..701
                     /locus_tag="VGB93_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0146941.1"
                     /translation="DTLRRIEVYDNSHIMGTNAVGAMIVAGADGFMKTHYRTFNIKGE
                     DLTPGDDFGMMREVLRRRFTRLAKEDEGANQDPEAFPQRPDLILIDGGKGQFEATRTV
                     LADLGVNDVAIVAIAKGLDREAGRETFFVEGKEPFRLPPRDPALYFVERLRDEAHRFA
                     IGTHRARRKKDFTRSPLDEIAGVGPARKRALLQAFGTAKAVAHAGLADLEKVDGINSA
                     TAKLVYDFFHGERA"
     gene            765..1364
                     /gene="pgsA"
                     /locus_tag="VGB93_12525"
     CDS             765..1364
                     /gene="pgsA"
                     /locus_tag="VGB93_12525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0146942.1"
                     /translation="MAVSSLTRGSSRRFSLPNLLTYGRVAAVPVVVGCLFWPEVAAAR
                     WAALAIFILAGISDFLDGYLARALAQQSSLGRMLDPIADKLLVSAVLLMLAADHTIAG
                     ASLWAAIVILCREILVSGLREFLAELKVSVPVSRVAKWKTFLQLVALGFLIAGPAVAP
                     VVTMIGLGLLWVSAILTFYTGGDYFIAGVKHLLDEDEKI"
     gene            1361..1612
                     /gene="moaD"
                     /locus_tag="VGB93_12530"
     CDS             1361..1612
                     /gene="moaD"
                     /locus_tag="VGB93_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY0146943.1"
                     /translation="MKARYFAWVRERIGLAEEEITLPSSVETVADLIAWQQQRGENYA
                     AAFANAATIRAALDRQHVKPGTKIADAAEVAFFPPMTGG"
     gene            1614..2063
                     /locus_tag="VGB93_12535"
     CDS             1614..2063
                     /locus_tag="VGB93_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY0146944.1"
                     /translation="MARRIIRVADKAFDIAVETEALELGSGTGALVTFTGLCRDEGGT
                     LTALEIEAYPEMAEAEIARVVDEAERRWPFDAVAIIHRYGRIRPGEAIVFVATASAHR
                     TEAFHAAEFLMDYLKTRAPFWKKAHRSSGSGDWVAAKDDDEAAAARW"
     gene            complement(2118..2702)
                     /locus_tag="VGB93_12540"
     CDS             complement(2118..2702)
                     /locus_tag="VGB93_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146945.1"
                     /translation="MCSKVALWASPALICGFMGISAFGLGAARADDAACKGIADAMLT
                     NSKTPYHSVGTIAFDPKDPPIPGAEAMPAIKTETIFTGTQVFVKLPTGQWKDIHANIA
                     DLRTKVKDSASNFNDCTRLPDEKADGKTLAVYTGGDKTDTMSVSTKVWVAGGAIQRSE
                     TDVGGPTAPDGKVRHQHLSLHYDYADIKAPTDSN"
     gene            2901..3170
                     /locus_tag="VGB93_12545"
     CDS             2901..3170
                     /locus_tag="VGB93_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146946.1"
                     /translation="MSETISRRQLALMSALAAAGTLGAARPAAAYQGNMENALGLLHE
                     ALDFLRQSTPNKGGHREHAMRLIRGAIDEVRAGIDFAAAHGGGGQ"
     gene            3285..3512
                     /locus_tag="VGB93_12550"
     CDS             3285..3512
                     /locus_tag="VGB93_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146947.1"
                     /translation="MKKLILASVLVVVACAPALADDTYSLVKDTVGNCSAAVTSRHYP
                     GMTIVGDKTYSSIEDANKALDDVKDCAGLVR"
     gene            complement(3596..3752)
                     /locus_tag="VGB93_12555"
                     /pseudo
     CDS             complement(3596..3752)
                     /locus_tag="VGB93_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          696 a         1151 c         1146 g          759 t
ORIGIN      
        1 aagatacgct gcggcggatc gaggtctacg acaattccca catcatgggc acgaatgccg
       61 tcggcgcgat gatcgtcgcc ggggcggacg ggttcatgaa gacgcattat cgcaccttca
      121 acatcaaggg cgaggattta acgcccggcg acgatttcgg catgatgcgc gaagtgctgc
      181 gccgccggtt cacgcgtctc gccaaagagg acgagggcgc caaccaggac cccgaggcgt
      241 ttccgcagcg acccgatctc atcctgatcg acggcggcaa gggccagttc gaggcgacgc
      301 ggaccgtgct cgccgatctc ggcgtcaatg acgttgcgat cgtcgcgatt gccaagggac
      361 tggatcgcga agccgggcgc gaaacatttt tcgtcgaggg caaagagccg ttccggctgc
      421 cgccgcgcga tccggcgctt tatttcgtcg agcggttgcg cgacgaggcg catcgcttcg
      481 ccatcggcac gcatcgcgcg cgccgcaaga aagacttcac ccgcagcccc ttggatgaaa
      541 tcgccggcgt cggcccggcg cgcaagcgcg ccctgttgca ggctttcggc acggccaagg
      601 ctgttgctca cgccggcttg gccgatctcg aaaaggtgga cggcatcaat tccgccacag
      661 cgaagcttgt gtacgatttc ttccacggcg agcgggcgta gcggcgccac accatcacgc
      721 atccttctat tgacctttcg cgccacggca tgttccttcg ccctatggcc gtgtccagtc
      781 tcacgcgcgg gtcaagccgc cgcttcagtc tgcccaatct cctgacctat ggccgggtcg
      841 ctgccgtccc ggtcgtcgtc ggttgcctgt tctggccgga ggtcgcggcg gcgcgctggg
      901 cggcgctggc gatcttcata ctggcaggca tcagcgattt tctcgacggc tatctggcgc
      961 gcgccctggc gcagcaatcg tccctcggac ggatgctcga cccgatcgcc gacaaattac
     1021 tcgtctcggc ggtgctgctg atgctcgccg ccgatcatac gatcgcaggc gcctcgctct
     1081 gggcggcgat cgtcatcctc tgccgcgaaa ttctcgtctc gggcctgcgg gaatttctcg
     1141 ccgaactcaa agtctcggtg ccggtgagcc gggtggccaa gtggaagacg tttctgcaac
     1201 tcgtcgcgct cggctttctc atcgccggcc cggcagtggc accagtcgtc acgatgatcg
     1261 gcctcggtct gctctgggtc tcggcgatcc tgacgtttta caccgggggc gactatttca
     1321 tcgccggggt caaacatctt ctcgacgagg atgagaaaat atgaaggccc gttatttcgc
     1381 ctgggtgcgc gagcgcatcg gcctggcgga ggaagagatc acgctgccca gctcggtcga
     1441 gaccgtcgcc gacctgattg cctggcaaca acagcgcggc gagaattatg ccgccgcttt
     1501 cgccaatgcc gcgacgatcc gcgcggcgct cgaccgccag cacgtcaagc ctggaacaaa
     1561 gatcgccgac gcggcggaag tcgccttctt cccgccgatg acgggcggct gacatggcac
     1621 ggcgcattat ccgcgtcgcg gacaaggcat tcgacatcgc ggtcgagacc gaggcgctag
     1681 aactcggctc cggcactggc gcgctcgtca ccttcaccgg gctttgccgc gacgaaggtg
     1741 gcacactcac ggcgctcgaa atcgaagcct atccggaaat ggctgaagcc gagatcgcgc
     1801 gtgtcgtcga cgaagccgag cgacgctggc cgttcgatgc cgtggccatc atccatcgct
     1861 atgggcgtat tcgccccggc gaagccatcg tcttcgtcgc gaccgccagt gcgcaccgca
     1921 ctgaggcgtt ccacgctgcg gaattcctga tggattacct caagacccgc gcgccgttct
     1981 ggaagaaagc gcatcgcagc agcggcagcg gcgactgggt cgccgcgaag gatgacgacg
     2041 aagctgccgc cgcgcgctgg tagactttgt tcaacgcata atctgacccg aaaagtctgc
     2101 aacttttcgg gattatgcta attcgaatcc gtcggcgctt tgatgtcggc gtaatcgtag
     2161 tgcagcgaca gatgctgatg ccgtactttg ccatcaggcg cggtcgggcc gccgacatcg
     2221 gtttcggagc gttggatagc accgccggcc acccacactt tggttgaaac actcatcgtg
     2281 tcggtcttgt cgccgccggt atagaccgcg agcgtctttc cgtcggcctt ttcatcgggc
     2341 aggcgcgtgc aatcgttgaa gttgctggcg ctgtctttca ccttggttcg caggtcggcg
     2401 atattggcgt ggatgtcctt ccactgtccc gtcggcaatt tcacgaagac ctgcgtaccg
     2461 gtgaagatcg tctcggtctt gatagccggc atagcctcgg cgcccggaat aggcgggtct
     2521 ttcggatcga acgcgattgt gccgacgctg tgatacggcg ttttggaatt cgtcagcatc
     2581 gcatccgcaa tgcctttgca ggcagcatcg tcggcccgcg cggcaccaag cccaaacgcc
     2641 gaaattccca taaatccgca gatcaaggcg gggcttgccc agagggctac cttcgaacac
     2701 attccaacac tccctattct tatcattccc ggtcgggctg gcttatatcc cggccatccg
     2761 ggatcgtcac ccctggcgcc agtcgcttta ttcttgggca atatcggggc gtggcagcga
     2821 tctcccgggg cagacccggc gctgccatgc tattttccag ttgcagctta caatactgcg
     2881 aggatgaggg gagattggga atgtcggaga caatatcgcg tcgccaactt gcgctgatgt
     2941 cggcgctcgc cgctgccggg acgttagggg ccgcgcgacc agcggccgcc taccaaggga
     3001 acatggagaa tgcgctcggt ttgctgcacg aggcgcttga ttttcttcgc cagtccacgc
     3061 cgaacaaggg cggccatcgc gagcatgcga tgcgattgat ccgcggtgca atcgatgagg
     3121 ttcgggcggg aatcgacttt gctgcggcgc acggcggcgg cggacagtga cgcgaagcga
     3181 tcgcgcgact tccgcttttt cccggatttt ttgcaatatt tctaattatc agagcagaaa
     3241 cttcatggaa ttctgcaccc gtttataaaa aatgggagga agctatgaag aagttgatac
     3301 tcgcgagcgt attggttgtc gttgcctgcg cgccagcact cgctgacgac acatattcgc
     3361 tcgttaaaga tactgtcgga aattgttctg ccgccgtaac atcgaggcat tatcctggca
     3421 tgacgatcgt cggcgacaaa acctattcgt cgattgagga cgccaacaag gcgctcgacg
     3481 acgtgaagga ttgcgccggt ctcgttcgat aacaagccag cgcaaagagc gtcagcgtct
     3541 ttatcgacgt tgacgcggcg ccgaagttag acggcgacct agtcgtccgt gaagaaggcg
     3601 tctcaagcgg tcgatcacac aaaacctcgt cggaaagatc gtgcatgtgc ttcaggatgg
     3661 cgagccccgc catcagcctg gtcgcaagcg gcggatgtcc cggcttgtcg ctgtaagcgg
     3721 taccgaagct tgtttcgaga aagccccaat cg
//
LOCUS       DASWOG010000611         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_362554,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000611 DASWOG010000000
VERSION     DASWOG010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362554"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..917)
                     /locus_tag="VGB93_12560"
     CDS             complement(87..917)
                     /locus_tag="VGB93_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0146948.1"
                     /translation="MRTVSACSSLVVLLLLVTTLAIVPAAAEVIAARYAVTLVGFRIG
                     EASARGAVSAAAYRVALDARLTGLAEWLAHMRIALASSGEIRRGAVLPNAYATTAASE
                     RDTRTVRMALTAGNVRAVDISPPLEDEAIRIPVSSAEKRNVLDPTSALIMAVPAGEAL
                     VGPAACNRTLQIYDGVVRFDLTMTYTGTRNVTAIGYDGPVSVCAVRYKPIAGHRYDSR
                     STRFMAQNHDIEVWLAPIERAHVVIPFHIGLRTLAGMATVDAVELHLEPSGKTLKADR
                     "
     gene            1287..1712
                     /locus_tag="VGB93_12565"
     CDS             1287..1712
                     /locus_tag="VGB93_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY0146949.1"
                     /translation="MSVNVLYRTKATAHGGRDGHSRTEDGTLDVSLTIPKELGGPGTP
                     GNNPEKLFAAGYSACFLGAMKFVASQGGPKVPENTVVSAIVGIGPRSEGGFGLEVELD
                     VSLPGLPHDEAEQLIEKADKVCPYSNALRGNVQVTLKLV"
     gene            1779..2753
                     /locus_tag="VGB93_12570"
     CDS             1779..2753
                     /locus_tag="VGB93_12570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0146950.1"
                     /translation="MDYTKLGRTGLEVSRICLGCMTFGEPGRGPHTWTLDEEKSRPII
                     KKAIELGINFFDTANVYSDGSSEEIVGRTLKDFASRQDVVIATKVHGRMHPGPNGSGL
                     SRRAILAEIDNSLRRLGVDYVDLYQIHRWDHSTPIEETLEALHDVVKTGKARYIGASS
                     MFAWQFAKALYISRLNGWTEFVSMQDHINLLNREEEREMLPLCRDQKIAVLPWSPLAR
                     GRLTRDWDETSERQNTDVFGKTLYGLESDKKTAGEVADVAKARGVPRAQVALAWLLQK
                     DGITAPIIGASKLNHLDDAVAALSLKLTAEEIAKLEAPYVPHPIVGFQ"
     gene            2764..>3180
                     /locus_tag="VGB93_12575"
     CDS             2764..>3180
                     /locus_tag="VGB93_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0146951.1"
                     /translation="MNWLITGVSSGFGRALSELILEKGGKVAGTVRKDTDKAEFEKLA
                     PGRAFGILLDVTDETAVHRGVKEAEEKLGGIEVLVNNAGYGFEGALEEATLADFRQQF
                     EVNVFGAIAMMQATLPYLRSRHAGHIINISSMGGLMT"
BASE COUNT          673 a          976 c          950 g          581 t
ORIGIN      
        1 acggactccg gcgctctgca ttgtcaaggc tacgatgaaa aatgatcgat cacttcaaag
       61 cgccgtgaat tggcgcgtac cgatcttcag cggtcggcct tcaaagtctt gccacttggc
      121 tccaaatgaa gctcaacggc atcgacagtg gccatgccgg ccaaggtccg cagcccgata
      181 tggaacggga tgaccacatg cgcacgctcg atcggcgcga gccaaacttc aatatcgtga
      241 ttttgcgcca tgaatctcgt cgagcgcgaa tcgtatcgat ggccggcgat aggtttgtag
      301 cgaacagcgc agacggacac cgggccatcg tagccgatgg ccgtgacgtt acgcgtcccc
      361 gtataggtca tcgtcagatc gaaacgcacc acgccgtcat agatctgcag cgtacgatta
      421 caggccgcgg gcccgaccag cgcctccccg gcgggcacgg ccatgatcag cgcgcttgtc
      481 ggatcgagaa cgttgcgttt ttccgccgac gagacgggaa tgcgaatggc ttcgtcttcg
      541 agcggcggcg aaatatcgac ggcgcgcaca ttgcctgcgg tgagcgccat ccgaacggta
      601 cgcgtatcgc gctccgacgc ggccgttgtc gcataagcgt tcggcagtac cgcgccgcgg
      661 cgaatctcgc ccgatgacgc aagcgcaatc cgcatatgtg cgagccactc ggccaaacct
      721 gtcaggcgcg cgtcgagcgc gacgcgatag gccgcggcgc tgacagcgcc ccgcgcgctg
      781 gcttcgccga tccggaaccc aactaacgtc acggcatagc gcgcggcgat gacttccgcc
      841 gcagcgggga cgatcgccag cgtcgtgaca agaagaagca ggacgacgag agacgagcaa
      901 gcggaaacgg tgcgcatggg agaccgtgta taaatccgag aggatgaaga caaaagcccg
      961 ctggtccctt gttcgtataa ttgctgagga cccaggacgt ttggcgggaa attcggcctc
     1021 tttcaaggcg ataacgatga gaatcgccgt agttcgccgg taattatcgc cgcttcgctg
     1081 cgttgatgca agaagtcctg cgactcagcc ggccagcgat ttcggcaata tccgacaagc
     1141 ggagcgccgc cggttcgttt atacacgcgt gaccttccgc ccgccgcaag gctacgttga
     1201 aaaccgctcc aatacgtttc atgtgaagag cgccgcttga aaacgcccgg cgccagcccg
     1261 cgtgaattca ccccaaggag taagccatgt cggtcaatgt tctttatcgc accaaagcga
     1321 ctgcccacgg tggccgcgac ggccattcgc gcacggaaga cggcacgctc gatgtctcgt
     1381 tgacaattcc gaaggaactc ggcggccccg gcacgcccgg caacaatccc gagaaacttt
     1441 tcgctgccgg ctattcggcc tgcttccttg gcgccatgaa atttgtcgcg tcgcaaggcg
     1501 gaccgaaagt tcccgaaaac accgtggtct cggcaattgt cggcatcggt ccgcgctcgg
     1561 aaggtggctt cggcctcgaa gtcgaactcg atgtttcgct gccgggcctg ccgcacgacg
     1621 aggccgaaca attgatcgag aaggcggaca aggtctgccc ctattccaac gctttgcgcg
     1681 gcaacgtcca ggtcaccctc aagctcgtct gagccgcgcg catttcacct tgacggccgc
     1741 ggttttcgcg gccgttcgtt tttccggaga ttgaagatat ggactacaca aagctcggcc
     1801 gcaccggact tgaggtctcg cgcatctgtc tcggctgcat gacctttggc gagcccggac
     1861 gcggcccgca tacctggact ctcgacgagg aaaagagccg gccgatcatc aaaaaggcga
     1921 tcgaactcgg catcaatttc ttcgacacag cgaatgtcta ttcggatggc tcgtcggaag
     1981 aaatcgtcgg acgcacactg aaggattttg cttctcgcca ggatgtcgtg atcgcgacca
     2041 aggttcatgg ccgcatgcat ccgggaccaa acggctccgg cctgtcgcgc cgcgcaattc
     2101 ttgccgagat cgataacagt ttgcgccgcc tcggcgtcga ttatgtcgat ctctatcaga
     2161 tccatcgctg ggatcacagc acgccgatcg aagagacgct tgaggcgctg catgatgtcg
     2221 tgaagaccgg caaggcgcgc tacatcggtg cgtcgtccat gttcgcatgg caattcgcca
     2281 aggcgctcta catctcgcgc ctcaacggct ggacggaatt cgtgtctatg caagaccaca
     2341 tcaatctctt gaaccgcgag gaagagcgcg aaatgctgcc gctctgccgc gaccaaaaga
     2401 ttgctgtgct tccctggagc ccgctcgcgc gcgggcgact gacgcgcgat tgggacgaaa
     2461 cgagcgaacg ccagaacacc gacgtcttcg gcaagacgct ttacggcctc gagtccgaca
     2521 agaagaccgc aggagaagtc gcggacgttg cgaaagcccg cggcgtgccg cgcgcgcagg
     2581 tcgcgctcgc gtggcttttg cagaaggatg gcatcactgc gccgatcatc ggcgcgtcca
     2641 agctcaacca cctcgacgat gcggtggcgg cgctgtcgct gaagctcacg gcggaagaaa
     2701 ttgccaagct cgaagcgcct tacgttccgc atccgatcgt cggctttcaa taaggagttc
     2761 gccatgaact ggctgatcac aggtgttagc agcggcttcg gccgcgcact gtcggaactg
     2821 atcctcgaaa aaggcggcaa ggtcgctggc acagtccgta aggatacaga caaagccgag
     2881 ttcgagaagc tcgcaccggg ccgcgcgttc ggcatcctgc tcgatgttac cgacgaaacc
     2941 gcggtgcacc gcggcgtcaa ggaagccgaa gaaaagctcg gcggcatcga ggtgctcgtc
     3001 aacaatgccg gttacggttt tgagggcgcg ctggaggaag cgacccttgc cgacttccgt
     3061 cagcagttcg aggtgaatgt cttcggcgcc atcgcgatga tgcaggcgac cctgccctat
     3121 ctgcgtagcc gccacgccgg ccacatcatc aacatcagct caatgggcgg gctcatgacc
//
LOCUS       DASWOG010000612         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_235734,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000612 DASWOG010000000
VERSION     DASWOG010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235734"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGB93_12580"
     CDS             <1..370
                     /locus_tag="VGB93_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0146952.1"
                     /translation="VGTAKNANIDGYFAGGKTGTADKIFHGHYSHDKVLTTFMAIVPA
                     DKPKYLFFVMLDEPQAIPGTYGFHTAAWNSGAVAGKVITRVGPLLGLEPRFTPPTQPF
                     PLLAQLGYTAANSAVVLGSH"
     gene            370..1827
                     /locus_tag="VGB93_12585"
     CDS             370..1827
                     /locus_tag="VGB93_12585"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY0146953.1"
                     /translation="MRLGDLLPEVALAAGSAAREIAGLSADSRAVGENFVFFALSGSK
                     ADGAVFAAQAVARGAVAVVGQHKVDVAPAIFIEVSDARRSLALAAACFYQQQPETIVA
                     ITGTSGKSSVAAFTRQIWLALGFRAASLGTIGLVAPSGEVYGSLTTPDPVSLHKTLDE
                     LARDGVSHLAFEASSHGLDQRRLDGVRLAAGAFTNLSRDHLDYHPDMDSYLAAKVRLF
                     DTLLQPGQAAVINADSDVADKVVAACEARGLRLLTVGRTGEAIKLLEMQPEDFATALT
                     LEHNGKTFSVRLPLAGDFQVQNALVAAGLVLATGGLADQVFAALEKLEGASGRLELVG
                     RRNDAPIFVDYAHKPDALDKVLRTLRPLARNKLIVVFGCGGDRDKGKRPIMGELAARL
                     ADEVIVTDDNPRSENPNHIRAEILAGARGVSGAKIEEIGDRARAIAAGVAALREGDVL
                     LVAGKGHETGQIVGDRILPFSDQESVRTALGVSAA"
     gene            1866..>2320
                     /locus_tag="VGB93_12590"
     CDS             1866..>2320
                     /locus_tag="VGB93_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY0146954.1"
                     /translation="MAVAPLWTPLALFAPMHARLSGTMPQPIFGVSIDTRTLMPGDLF
                     FAIKGDRSDGHDHVRDAFDKGAAAAVVDEDHSDTLKGAGPLYVVHDVLRAMEGLAVAA
                     RARTSARIVAVTGSVGKTTTKEALRHVLSQAGATHASAASYNNHWGVPLT"
BASE COUNT          400 a          695 c          745 g          480 t
ORIGIN      
        1 ggtcggcacg gccaagaatg ccaatattga cggctatttc gccggcggca aaactggcac
       61 cgcggacaag attttccacg gccattattc gcatgacaaa gtcctgacga ccttcatggc
      121 gattgttccg gccgacaagc caaagtatct tttcttcgtg atgctggatg aaccgcaagc
      181 aatccccgga acttatggat tccacacggc ggcgtggaac tctggcgccg ttgcgggcaa
      241 ggtcatcacc cgcgtcgggc cgctgctcgg gcttgagccg cgtttcacgc cgccgaccca
      301 gccatttccc ctacttgccc agctcggcta taccgctgcg aattccgcgg tcgtgcttgg
      361 gagccattga tgcgccttgg cgatctcctg cctgaggtgg cgctcgccgc cggatcggct
      421 gcgcgcgaga tcgcagggct cagcgccgac agccgcgctg tcggcgagaa tttcgtcttt
      481 ttcgccttgt cgggcagcaa ggcggacggt gcggttttcg ccgcgcaggc cgtcgcgcgc
      541 ggtgcggtcg cggtcgtcgg ccagcataag gttgatgtcg cacccgccat tttcatcgaa
      601 gtgtccgatg cacggcggtc cttggcgctt gccgccgctt gcttttatca gcagcagccc
      661 gaaacgattg tcgctatcac tggcacaagc ggcaaaagct cggttgcggc cttcacgcgg
      721 caaatctggc tcgcgctcgg ctttcgtgcc gcttcgctcg gcacgatcgg cctcgtggcg
      781 ccatccggcg aagtctatgg ctcattgacg acgcccgatc cggtctcgct gcataaaacg
      841 cttgacgagc ttgcgcgcga tggcgtcagc caccttgctt tcgaggcttc atcgcacggg
      901 ctcgatcagc gccggcttga tggcgttcgc ttggccgcag gcgctttcac caatctgtca
      961 cgcgaccatc tcgattatca tccggatatg gactcgtacc ttgccgcgaa agtgcggctg
     1021 ttcgatacat tgttgcagcc ggggcaagcg gccgtcatca atgccgacag cgatgtcgcg
     1081 gataaagtgg ttgcagcttg cgaggcgcgc ggtctgcgcc tgctcacagt cggtcggacg
     1141 ggcgaggcaa tcaagttgtt ggagatgcag ccggaagatt tcgccaccgc gctgacgctt
     1201 gagcacaatg gcaaaacctt ttccgtccgt cttccgcttg cgggcgattt tcaggttcag
     1261 aacgcactgg tcgcggcggg tctcgttttg gcgaccggcg gtctggcaga tcaggttttc
     1321 gccgcgttgg agaagctcga aggcgcttcg ggccgtcttg aactcgtcgg ccgccgtaat
     1381 gacgcgccga tcttcgtcga ttacgcgcat aagccggatg cactcgataa agtactccgc
     1441 accttgcgac cactggcaag aaacaagctc attgtcgtct tcggctgcgg tggtgatcgc
     1501 gacaagggca agcggccgat catgggcgaa ttggcggcgc ggcttgccga cgaggtgatc
     1561 gtcaccgacg acaatccgcg ctcggaaaat ccgaaccaca tccgtgccga aattctggcc
     1621 ggtgcacgcg gcgtttcggg tgccaaaatc gaggagattg gcgaccgggc gcgggcgatt
     1681 gctgcgggcg tcgcggcgct gcgagagggc gacgtgctgc tcgtcgccgg aaaaggccat
     1741 gaaacgggcc agatcgtcgg tgacaggata ttaccgtttt ccgatcagga gagcgtgcgc
     1801 accgccttgg gagtttccgc ggcatgagtt tcgacgtgtc gaaggaaagt gcctttgcgt
     1861 ccgagctggc cgttgcgccg ctgtggacgc cgctggcgct gttcgcgccg atgcatgcgc
     1921 ggttgagcgg aacgatgccg caaccgatct ttggcgtttc aatcgataca cgtacgctga
     1981 tgcccggcga tctcttcttc gcgatcaagg gcgaccgcag cgatggacac gatcatgtcc
     2041 gtgacgcgtt cgacaagggc gctgccgcgg cggtggtcga tgaggaccat tccgacacgt
     2101 tgaaaggcgc ggggccgtta tatgtggtcc atgatgtctt gcgggcgatg gaagggcttg
     2161 ccgtcgcggc gcgggcgcgc acatcggcgc gtattgtcgc cgtcacgggc tcagtcggta
     2221 agacgacgac aaaagaggct ttgcggcacg ttctttcgca agccggcgcg acacatgcgt
     2281 cagccgcgtc ctacaacaat cattggggtg tgccgctgac
//
LOCUS       DASWOG010000613         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_362605,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000613 DASWOG010000000
VERSION     DASWOG010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362605"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGB93_12595"
     CDS             <1..274
                     /locus_tag="VGB93_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146955.1"
                     /translation="AKHGLRRIRSIGEAAIAEGKASKIFTRKLRRRFLSSRNRNFSSY
                     ARNSAKAFRSKGTLLQIRRFERMVEIELAQLNNVVDETSQPPKPQT"
     gene            complement(357..1100)
                     /locus_tag="VGB93_12600"
     CDS             complement(357..1100)
                     /locus_tag="VGB93_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0146956.1"
                     /translation="MVRAAEAAQCGTGPGGFEAWKSAFADEAKAKGIGNAGVSALMQT
                     HYNYPTIAADRGLKSFRLSLPAFMAKRGASIIASRGRRMKEAHADLFASIEEQYGVPA
                     GPLIAIWGMESGFGTQHGNQQSLSAITTLAYDCRRPDFFTDQFDAALKLVDDGRLTAE
                     TRGSMHGEIGQTQFLPKTVLQYGVGDLDTAEGALASTANYLKAHGWKPGAGYQPGEPN
                     FAAIQAWNAATVYEQTIAIVGKEIDGGDD"
     gene            complement(1338..1889)
                     /locus_tag="VGB93_12605"
     CDS             complement(1338..1889)
                     /locus_tag="VGB93_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0146957.1"
                     /translation="MGPPLLIDKKADFAIAQMPTIVLYLGETLHLLPATAALRALTMK
                     IVNDANDVIDEITLDGGREMWTDKRWRDFTPRLEKWMSFWEETGRRHGLKAKSGFLLG
                     GDKPGIADVVTATLWSTMTDRFHEIETMLKETAPQTAALTRRIANLPPLAELAAKAQI
                     DYGDSYCGGQIEASLRKVLGAER"
     gene            2220..3611
                     /locus_tag="VGB93_12610"
     CDS             2220..3611
                     /locus_tag="VGB93_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0146958.1"
                     /translation="MAYTSTNPYTGEVTKTFHDATDTEVVQAIEKAHEAFLSWKKTSF
                     PARAKIMQRAADILRRDSEAYAKLLTLEMGKLISEAEAEVELSAKIFEYYAINARTLL
                     APEKLPVADLAEGEAVLVHEPLGVLLAVEPWNFPYYQVARIISPQLSAGNTILLKHAG
                     IVPQSAAACEKLMLEAGLPEGAFKNLYAAHSQIDLILQHPLVRGVALTGSEGAGAAVA
                     VAAGQALKKSTLELGGADAFVVLADADLEKTVKWAVFGRHWNGGQVCVSSKRMIIVKD
                     LYDRFLENYTAGVAGLRVGDPFDPATTLAPLSSQSAADELKDRISQAVAHGATATEVG
                     PKVPNRGAFVQPTILTNITDDNPARNWEFFGPVSMLFRAEDEDDAVRIANDSPFGLGG
                     SVFTSNPKHGAKVAKRISTGMVFVNHPTMVKADLPFGGIRNSGYGRELLGLGIKEFVN
                     HKLIDIVDIDAPF"
     gene            3824..3985
                     /locus_tag="VGB93_12615"
     CDS             3824..3985
                     /locus_tag="VGB93_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146959.1"
                     /translation="MRDDPENIVAFRHSCGAKLHLEMRCAACDEAIIPGDVTGDRDVR
                     RSDIVCTGA"
     gene            complement(3995..>4768)
                     /locus_tag="VGB93_12620"
     CDS             complement(3995..>4768)
                     /locus_tag="VGB93_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0146960.1"
                     /translation="RIHSYAVWESAVFLLNVFAFLLLGCQARTIVGGMAPAHLRDAAW
                     FALAVILCLIVVRMALVLGYNRLALRYRIFRADAKAASLRQGILVGWSGMRGLVTLAT
                     AFALPASFPQRDLIVLTAFSVVLATLVIQGLTLGPLVRLLKLDGDDGLGAELDSARVD
                     LASVALASLDGKDGPAADYWRYEFETLRDGAILEGELAPLEMKRQYGFAAVRKQRKRL
                     DKLRIEGRVGPDAFVVLQEELDFAEVALNLETDQHIEES"
BASE COUNT          960 a         1401 c         1450 g          957 t
ORIGIN      
        1 agctaaacat ggcttgcgtc ggattagaag cattggcgag gcggctatcg cggaaggaaa
       61 agcttcaaaa atttttacaa gaaaacttcg acgtcggttt ttatcttcgc gaaatagaaa
      121 cttctcaagt tatgcgcgca attccgcgaa agcctttaga tcgaaaggaa ctctccttca
      181 gattcgtcga ttcgagcgaa tggtagaaat cgagctagct caattaaata atgtcgtgga
      241 tgagacttct caaccaccaa agcctcagac gtgattggct aatctacgcg atgatataac
      301 cgatcattcc tttttgagat tagcaacttc gctttggccg gagttctaga agcccctcaa
      361 tcgtccccgc catcgatctc tttgccgaca atcgcgatcg tctgttcata gacggtcgct
      421 gcgttccatg cttggatcgc cgcgaaattg ggctcgcccg gctgatagcc ggcgcccggc
      481 ttccagccat gcgccttgag atagttcgcc gtcgaggcca gtgcgccttc ggcggtgtcg
      541 agatcgccga cgccgtattg cagcaccgtc ttcggcagga attgcgtctg gccgatttcg
      601 ccgtgcatgg agccgcgcgt ctccgctgtg aggcgcccgt catcgaccag tttcagtgcc
      661 gcatcaaact gatcggtgaa aaagtccggc cggcggcagt cgtaggcgag ggtcgtgatg
      721 gccgagagcg attgctggtt gccgtgctgc gtgccgaagc ctgattccat gccccagatg
      781 gcgatcagcg gcccggccgg gacgccatac tgctcttcga tcgaggcgaa gaggtcggca
      841 tgcgcttcct tcatcctgcg cccgcgcgat gcgatgatag aagcgccgcg cttggccatg
      901 aaggccggaa gcgacagacg gaagcttttc aggccacggt cggctgcgat ggtcggataa
      961 ttgtagtggg tctgcatcag ggcgctaacc cccgcgttgc caatgccctt ggctttcgcc
     1021 tcgtcggcga aggcgctctt ccaggcctcg aacccgcctg ggccggtgcc gcactgggcg
     1081 gcctccgcgg cgcgaaccat caagggaagg gcggcaaagg ccgcgattaa gcttgtggtg
     1141 agccggccgc agttaaacat tcaacctccc atttagagcg ttttcgagcg aaggggatac
     1201 cggttcgcgt gaagaaaacg cgttgaaaca aaaaactaga gcagaggacg ttcatatgga
     1261 atcgcccggc gttcttgtgt ctttttaacc gcatcggatt tgtccgaaaa ccgcttcgca
     1321 cttttcggtc cgatgctcta gcgctctgca ccgaggactt tgcgcaatga agcctcgatc
     1381 tgtccgccgc aatacgaatc tccataatcg atttgcgcct tggcggccag ttcagccagc
     1441 ggcggcagat tggcaattcg ccgtgtcagg gcggccgtct ggggcgcggt ttccttaagc
     1501 attgtctcaa tctcgtggaa gcggtcggtc atggtcgacc agagcgtcgc ggtcacgaca
     1561 tcggcgatgc ccggcttgtc gccgccgagc aggaagccgg acttggcctt caggccatgt
     1621 cgacgaccgg tctcttccca gaacgacatc cacttttcca ggcgcggggt gaagtcgcgc
     1681 cagcgcttgt ccgtccacat ctcgcggccg ccatcgaggg tgatctcgtc gatcacgtcg
     1741 ttggcgtcgt tgacgatctt catggtcagc gcgcgcaagg cagcggtggc gggcaggagg
     1801 tgcagcgtct cacccagata aaggacaatc gtcggcatct gggcgatggc gaaatccgcc
     1861 tttttatcga tgagcagcgg cggccccatg aaaggcaccg gcattttgct gatgggcccc
     1921 tccatcagct tggtgatcgc ggcgtcgccg gcttccgtcc atgtcttccc cgcataggcc
     1981 aggacggcgc gcacgaattg gccgcggaac ggcacggacc agtagtagag ttagtagtcg
     2041 gtcatagcgg agccctatat gtttgatgag cttcaatctt cactaactcg cgcaaactac
     2101 gcgcgcgcgt tgatccagat taaggcggcg cggaaatctt cgtgccttcg taaagcgaat
     2161 tggcgggccc gcgcacggcg cggcgccgat cttatgctgt ttcgcgacgg agatgagcga
     2221 tggcctatac gagcacaaat ccctacaccg gcgaagtgac gaagacgttt cacgacgcca
     2281 cggacacgga agtcgtccag gccatcgaaa aagcgcacga agcatttctg tcgtggaaaa
     2341 agacatcatt tccggcgcgg gcgaagatca tgcagcgggc tgcggacatt ctgcgcagag
     2401 acagcgaagc ctatgcaaag cttctcacac ttgaaatggg gaagctgatc tcggaggcgg
     2461 aggcggaggt cgaactctcg gccaaaatct ttgaatatta tgccattaac gcaaggaccc
     2521 tgctcgcgcc ggaaaagctg ccggttgccg atcttgccga aggcgaggct gtgctggttc
     2581 atgaaccgct cggcgtgttg ctagcggtcg aaccgtggaa ttttccgtat taccaggtcg
     2641 cgcgtatcat ctcgccgcaa ttgtcggccg ggaatacgat cttgctaaag cacgccggaa
     2701 tcgtgccgca gtccgctgcc gcttgcgaaa agctcatgct ggaagcggga ctgccggagg
     2761 gcgcattcaa aaatctttat gccgcacatt cgcagatcga tcttatcctg cagcatccgc
     2821 tcgtgcgcgg cgtcgcgctc accgggtctg aaggcgccgg cgctgcggtt gctgtggcgg
     2881 ccgggcaggc gctgaagaaa tcgactttgg agcttggcgg cgcggatgcc tttgtcgtgc
     2941 tggcagatgc cgatctggag aagacggtca aatgggcggt cttcggtcgt cactggaacg
     3001 gcggacaggt gtgcgtctca tccaagcgga tgattatcgt caaggatctc tacgaccgct
     3061 tcctcgagaa ctataccgcc ggcgtcgcgg gcttgcgggt aggcgatccg ttcgatcccg
     3121 cgacaacctt ggcgccgctg tcctcgcaaa gcgccgctga tgagctgaaa gaccggataa
     3181 gccaggcggt ggcacatggc gcgacggcga cggaagtggg gcctaaagtc cccaatcgcg
     3241 gcgccttcgt acagccgacg attttgacca atatcaccga cgacaatcca gcgcggaatt
     3301 gggagttttt cggtccggtg tcgatgcttt tccgcgccga ggacgaggac gacgcggtgc
     3361 ggatcgccaa cgattccccg ttcgggttgg gcggatcggt gttcacctcc aatccgaaac
     3421 acggcgcgaa ggtcgccaaa cgaatctcga ccggcatggt tttcgtcaat catccgacga
     3481 tggtgaaagc cgacttaccc tttggcggca tccgcaattc aggctacggc cgcgaattgc
     3541 ttggtcttgg catcaaggag ttcgtcaatc acaagctcat cgatatcgtc gatatcgacg
     3601 cgccgttctg agacgtcgat cccagggcgt cagctctcct tggcctcata ctccgccttt
     3661 cgaaatgagg cgatgctctc aggcttccct gcctcctatt tcggaatcgc cgcgttcgcc
     3721 gagaccgacg ttaccgagga tctcaacacg ctgccacctg gccgaggcgg gtgaacagct
     3781 cttcgctgtc ctgcacgcaa tccgcgattg gggcgaccgt tttgtgcgtg acgatcccga
     3841 gaacatcgtg gcctttcgcc attcctgcgg agccaagctt catctcgaaa tgcgctgcgc
     3901 cgcctgtgac gaagcgatca ttccgggaga cgtaacgggc gaccgggatg tgcgccggtc
     3961 ggatattgtc tgcaccgggg cgtgatcgag cccttcagct ctcttcgatg tgctggtccg
     4021 tctccaggtt gagtgcgacc tcggcaaaat cgagttcctc ttgcaggacg acgaaagcgt
     4081 ccgggccgac gcggccttcg atacggagct tatccagccg tttgcgctgc ttgcgcaccg
     4141 cggcgaagcc atattggcgc ttcatctcaa gcggtgccag ctcgccttcg aggattgcgc
     4201 catcgcgcaa cgtctcgaac tcgtagcgcc agtaatcggc ggcgggtcca tccttaccgt
     4261 caagagacgc gagcgccacg gaggcaagat cgacgcgcgc ggaatcaagt tccgcgccga
     4321 ggccatcgtc gccatcgagt ttgagcagtc ggacgagcgg cccgagcgtc agcccctgaa
     4381 tcacaagcgt cgccagcacg acggagaagg cggtgagcac gatgaggtcg cgctgcggga
     4441 acgaggccgg gagcgcgaag gccgtcgcca gcgttacaag cccgcgcatg ccggaccatc
     4501 cgaccaaaat cccttgacgc aagctcgccg ccttggcatc ggcgcgaaat attcggtagc
     4561 gaagggcgag gcgattgtac ccgagcacga gtgccatccg cacgacgatc aggcacaaga
     4621 tgacggcgag cgcgaaccag gcggcgtcgc gcaagtgggc aggggccatg ccgccgacga
     4681 tcgtgcgcgc ctgacagccc aacagcagga aggcgaacac gttgagcaga aacaccgcgc
     4741 tttcccagac cgcataacta tggatacg
//
LOCUS       DASWOG010000614         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_69684,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000614 DASWOG010000000
VERSION     DASWOG010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69684"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VGB93_12625"
     CDS             complement(<1..1021)
                     /locus_tag="VGB93_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0146961.1"
                     /translation="MTDLTELSLAAARDALVKKEVSAVELTKAQIAAMEQGRALNCFI
                     VETPEKALAMAAQSDARLAAGAARPLEGIPLGIKDLYCTEGVQTTASSHILEGFVPTY
                     ESTVSANLWRDGALMIGKLNLDEFAMGSSNETSYFGPVVSPWRRPGSNVKIVPGGSSG
                     GSAAAVAARLCYGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAG
                     PIARNVRDAAILLRSMAGYDPKDSTSVNRPVPNYEAAIGTSIAGKIIGIPKEYRLDGM
                     PAEIAKLWDDGVAWLKDAGAKVVEISLPHTRYALPAYYIVAPAEASSNLARYDGVRYG
                     LRVPGK"
     gene            complement(1070..1357)
                     /gene="gatC"
                     /locus_tag="VGB93_12630"
     CDS             complement(1070..1357)
                     /gene="gatC"
                     /locus_tag="VGB93_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0146962.1"
                     /translation="MAVDQATVRRIAHLARIKLADAEVPHLADELNSILAFVEELSSV
                     DVSGVEPMTSVMPMHLHERVDVVSDGEIADQIVANASVTEDHFFVVPKVVE"
     gene            complement(1422..>1737)
                     /locus_tag="VGB93_12635"
     CDS             complement(1422..>1737)
                     /locus_tag="VGB93_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0146963.1"
                     /translation="MGIVITPDTGPVIFHMGDTAIFSDMALIRELYRPKIGIVPVGDR
                     FTMGGRIAAEAVHRYFDFETAIPCHYGTFDMLAQDPGEFVAALNGKPKTWVPAIGETL
                     SV"
BASE COUNT          358 a          566 c          508 g          305 t
ORIGIN      
        1 ccttgcccgg cacgcgcagc ccatagcgca cgccgtcata gcgggcgaga ttggacgagg
       61 cctcggccgg cgccacaata taataggcgg gcagcgcata gcgcgtgtgc ggcagcgaaa
      121 tctcgacgac cttggcgccg gcatccttca gccacgcgac gccatcgtcc cacagcttgg
      181 cgatctccgc cggcatcccg tcgagccgat attctttcgg aatgccgatg atcttgcccg
      241 cgatcgacgt gccaatcgcg gcttcgtaat tcggcaccgg ccgattgacg gacgtcgaat
      301 ccttcgggtc atagcctgcc atcgaccgca acaggatcgc cgcatcgcgg acattgcgcg
      361 caatcggacc ggcttgatcg agtgacgatg cgaatgccac gatgccccag cgcgaacagc
      421 ggccatacgt cggcttaatg ccgacagtgc cggtgaacgc agcaggctgg cggatcgagc
      481 cgccggtgtc ggttgctgtc gcgccataac aaagacgcgc agcgaccgcc gccgccgaac
      541 cgccggacga accgcccggc acgatcttga cgttcgagcc ggggcgccgc cacggcgaaa
      601 cgaccggacc gaaataagac gtctcattcg atgagcccat cgcgaactca tcgagattga
      661 gcttgccgat catcaaagcg ccatcacgcc acagattggc cgagacggtc gattcatagg
      721 tcggcacgaa gccttcgagg atgtggctcg atgcggtcgt ctgcacgccc tcggtgcagt
      781 aaaggtcctt gatgccgagc gggatgcctt cgaggggccg cgccgcgccc gccgcaagcc
      841 gcgcatcgga ttgggccgcc atcgcaagcg ccttctccgg cgtctcaacg atgaaacaat
      901 tgagcgcccg gccctgctcc atcgccgcga tctgcgcttt ggtcagttcg acggcggaca
      961 cctctttctt caccagcgca tcgcgcgccg cagcgaggct taattcggtc aaatcggtca
     1021 aaagttgctc tctatatatg tagatatatg ccgatctctg gaaacggctc tattcgacga
     1081 ccttcggcac gacgaagaaa tgatcttcgg tcacggaggc gttggcgaca atctggtcgg
     1141 caatctcgcc atcgctgacg acatccacac gctcatggag atgcatcggc atcaccgagg
     1201 tcatcggctc cacgccgctg acatcgacgc tgctcaattc ttcgacgaag gcgagaatcg
     1261 aattcaattc gtcggccaga tgcggcacct ccgcatcggc gagcttgatc cgggccagat
     1321 gggcaatgcg gcggacggtc gcctgatcaa cagccataat gtctcctcag aagagccgag
     1381 cctatagcat ccagccgccg gtgcgggcaa cgaaagacgc ctcagacgct cagcgtctcg
     1441 ccgatggcag gcacccaggt cttcggcttg ccattgagcg cggcgacgaa ctcgcccgga
     1501 tcctgggcca acatatcgaa ggtcccgtaa tggcagggaa tcgccgtctc gaaatcgaaa
     1561 tagcgatgca cggcctccgc cgcgatcctg ccgcccatgg tgaagcggtc gccgaccggc
     1621 acgatgccga tcttcggtcg gtaaagctct ctgatcaaag ccatgtccga gaaaatcgct
     1681 gtatcgccca tatgaaagat caccggcccc gtgtcggggg taatgacgat ccccatc
//
LOCUS       DASWOG010000615         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_87846,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000615 DASWOG010000000
VERSION     DASWOG010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87846"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..351)
                     /locus_tag="VGB93_12640"
     CDS             complement(100..351)
                     /locus_tag="VGB93_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146964.1"
                     /translation="MDELNGRMIACQILLTGLIAKVANNSPEPLRFLTEFRDEVRAVI
                     NQIRISGSHDTTRVRQSALATADELLGLMKRPSTVEAED"
     gene            complement(611..1621)
                     /locus_tag="VGB93_12645"
     CDS             complement(611..1621)
                     /locus_tag="VGB93_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY0146965.1"
                     /translation="MGAFILLLHVAGWGTLLGIVARQHLSIGNSVFGFGIGLAAYALG
                     MRHAFDADHIAAIDNTTRKIMQTGKRPLSVGFWFSFGHSTVVFVLALLLAFGVKSIAG
                     PVLDDKSQLHQVTGLIGTIVSSGFLCVIAIFNLMVLFDIIRVFRQMRRGQWSESVSDT
                     HHVRGIMSTLLRRVMNFVNEPWQMYFIGLLFGLGFDTATEIALLVLTGSGAASGLPWY
                     AILCLPVLFAAGMSLFDTIDGSFMTVVYAWALTKPVRRVYYNFVITGLSIAVALVIGT
                     IEIMGLLGDTLDLHGAIWAWVRTADLGNIGFVIVGLFVVTWIVAVATWKWGRIEDRLT
                     TP"
     gene            complement(1797..>2264)
                     /locus_tag="VGB93_12650"
     CDS             complement(1797..>2264)
                     /locus_tag="VGB93_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146966.1"
                     /translation="GGWRSINRIVLPMIHPLFTQYNEDLGNRLNAGRPSEDFATYGAL
                     VAKSIAGAVLATGTSVDPQAYGERVARRFFPNLLPYKIGTSATFGFAEWNGRSLTDNA
                     PDVMFSIAANAAVRLEIGKESVSPKPSATFPYVPDVGREHTSTSIPPGTMGPA"
BASE COUNT          523 a          626 c          647 g          468 t
ORIGIN      
        1 tcagccccca ttcccgcgct tgacgcgcgc cccagataaa tttcaaactt cggttgttgt
       61 cgttttctta ttcgtcgctt cgattgatgc ttatcgaaat cagtcttccg cttcgacggt
      121 gcttggccgt ttcatcaatc ctaacaattc gtcggctgtc gccaaagctg attgccggac
      181 ccgcgttgtg tcgtgggagc ccgaaatccg aatctggttg atgaccgcac gaacctcatc
      241 gcggaattcc gtcagaaacc gcaaaggctc gggcgaatta ttcgcgactt tagcgatgag
      301 ccccgtcaga agaatctgac atgcgatcat ccgcccattg agttcgtcca aagccccacg
      361 tccaatactt agaagaattc tagaaatgac ttttttgtca ggagcgtttt gactcggctg
      421 gtgaggttta acaacacgcg gccgctaaat agtaattaga ataactctag ttttggggtg
      481 ctggcctgct gatatagagg ctgtttcggt ggtagattag aatagattgt cgaggctcat
      541 ccatcctttg atggggcagt gtggctgatt gcccacatgc tcgtggctat ctcacgcgag
      601 tcgtttcgta tcagggcgtc gtaagccgat cctcgatccg gccccatttc caggtcgcaa
      661 ccgcgacaat ccatgtgacg acgaataatc cgacaatgac gaagccgata ttgccgagat
      721 cggctgtcct gacccaagcc cagatggcgc cgtgaagatc cagcgtatcg ccgagtagac
      781 ccatgatctc gatcgtgccg atgacaagcg caacggcgat cgaaagaccg gtaatgacaa
      841 aattataata gacgcgccgc acgggcttgg tgagcgccca tgcgtaaacg acggtcatga
      901 acgatccatc gatcgtgtcg aagagcgaca tgcccgcagc gaaaaggacc ggaaggcaaa
      961 ggatcgcgta ccagggcagt ccggacgcgg cgcccgatcc cgttaatacg aggagtgcga
     1021 tctcagtcgc cgtgtcaaac ccgaggccga agagaaggcc gatgaaatac atctgccatg
     1081 gctcgttcac gaagttcatc acgcgccgta gcagcgtgct catgatgccg cgcacatgat
     1141 gtgtgtcaga aacgctttcc gaccattggc cgcgccgcat ctggcgaaat actcttatga
     1201 tgtcgaacag aaccataaga ttgaaaatgg cgattacgca aagaaagccg ctcgagacaa
     1261 tcgtaccgat cagccccgtg acctgatgaa gctgagattt gtcgtcgaga accggccctg
     1321 cgatgctttt tacgccgaac gcgagcagca acgctaacac gaagacgaca gtcgaatgac
     1381 cgaacgaaaa ccagaagccg accgaaagag gacgtttgcc ggtctgcatg atcttgcggg
     1441 tggtattatc aatggccgcg atgtgatcgg catcgaaggc gtggcgcatt cccagcgcgt
     1501 aggcggcaag accgatgccg aaaccgaaga cgctattgcc gatgctcaag tgctggcgcg
     1561 cgacgatccc tagcaacgtc ccccagccgg cgacatgcag aagcagaata aaggcgccca
     1621 tgctgaccag gttccaccgc gcgcgattgc tttgacttga atccgcacgc ggttgggatg
     1681 acgaggactc gttaatcccg gcgatcggca tagccgctcc ttcggtttat gtcactgcga
     1741 caaaaatcga caatttgaac ggcgtgcggg gcctgcctgc acaccggaat taatgcctag
     1801 gccggaccca tcgtaccggg cgggatggac gtcgatgtgt gttctctgcc gacatcgggg
     1861 acatagggga aggtcgccga cggctttggc gaaaccgact ccttgccgat ctcaagccgg
     1921 acggccgcgt ttgccgcaat cgagaacatc acgtcgggcg cattgtccgt gagtgagcgg
     1981 ccgttccatt cggcgaagcc gaatgtcgcg gatgtcccga ttttgtaagg cagcaggtta
     2041 gggaagaagc gccgcgcaac tcgttcgcca taggcctgcg ggtccactga tgttccggtc
     2101 gccaacacgg cgcccgcgat ggacttcgcg acgagcgcgc cgtaggtcgc aaaatcttcg
     2161 gatgggcgtc cggcgttgag ccgattgccg aggtcctcat tgtattgcgt aaagagcgga
     2221 tggatcattg gcaggacaat gcggttgatc gagcgccagc cgcc
//
LOCUS       DASWOG010000616         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_309422,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000616 DASWOG010000000
VERSION     DASWOG010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309422"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /locus_tag="VGB93_12655"
     CDS             complement(<1..2526)
                     /locus_tag="VGB93_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY0146967.1"
                     /translation="MRAVAGFLCLLLVLAVSGRAETLQSDYRNADLASDAVRLAAQVA
                     QDGDDLTDQTPDALRQAAAAALAKRDSKGMLHALAGLIAINPNDATSWLAYSRAEVAS
                     GNSDDILQAATTAAYLAYLKAADKRQAALALAQLGAIFAQRESWRPSLDAYHASLGLV
                     DDLAVRTTYQKERDTYGFRILDYKVDKDSAAPRACFQLSESLAGGHVYFASYVSVAGI
                     DRPAVSAEDQQICVDGLQHGAHYTIVLRQGIPSAVNEQLLRTATYNIYVRDRSQQVHF
                     TGKNYVLPRVGQQGIPVVSVNVKKIAVQILRVGDRGLLPIITSSDFLSQLSSYRLDQL
                     KNSDARKIWKGTLDVASLLNKDVTTAFPVLEAAGALQAGVYIMTAKPGDGLQSSDSDD
                     DSNVATQWFVVSDLGLTALLSRDGLHVFVRSLTSAKPLAGISLRLVARDNEVLATKQT
                     DANGYVRFEPGLSRGSNGLAPGLIVGEDGKGDYGFLNMQQGAFDLTDRGVSGRDVTQP
                     LDAEVFTERGVYRSSETVYVTALLRDAKGSAKTGVPLTLVAKRPDGVEYKRVLIADQG
                     AGGRSYGLTLLPSSASGTWRVEAYVDPKGAPVGEATFLVEDYVPERLDFKLKPQETQA
                     RAGDSSTVDANARYLYGAPGSGLDISGDVTVEAAGNHGLPILQGYEAGLQDQTFTAVS
                     KDLPTSVTTDAKGDAKIDVPIPDVAATQPLEAKVTLRVGEPGGRAVERSVTLPILPKG
                     GLIGVKQDFVTLGDGDIASFDVIAVNSSAARVARKGVTWSLYRLLTDYQWYNQDGHWD
                     FETVKSSRRVASGTLDLAADALGKISAPVELGDYRLDVTSADPN"
     gene            2876..>3347
                     /locus_tag="VGB93_12660"
     CDS             2876..>3347
                     /locus_tag="VGB93_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146968.1"
                     /translation="MAINEEVRPERMKGRRGLGGSLPQKPNLHSWLASFSAEGGGEDH
                     KRRRQIATYIGACFSLGIAALAIFVLIHTLAHINPGQLRAAFDATENNQILAALGLTA
                     LSFLALTGYDGVALLQLRQKVPYTTTALASFTSYAICFTLGFPLITAGTVRFWIY"
BASE COUNT          661 a         1063 c         1012 g          611 t
ORIGIN      
        1 gttcggatcg gcgcttgtga catcgagacg ataatcgccg agttcgacgg gcgccgagat
       61 tttacccaaa gcatcagcgg caagatcgag cgtcccggaa gccacgcggc gcgaggattt
      121 caccgtctcg aaatcccaat gcccatcctg attgtaccat tggtaatctg tcagaagccg
      181 atagagcgac caggtcacgc ccttgcgtgc gacgcgcgcc gcgctggaat tgacggcgat
      241 cacatcgaac gaggcgatgt cgccatcgcc aagcgtcacg aaatcctgct tcacgccgat
      301 caggccgccc ttcggcaaga tcggcagcgt gacactgcgc tcgacggcgc ggccgcccgg
      361 ctcaccgacg cgcaaggtaa ccttcgcttc caagggttgc gtggcggcga cgtccgggat
      421 cggcacgtcg attttcgcgt cgcccttggc gtcggtcgtc acgctcgtcg gcagatcctt
      481 actgaccgcg gtgaacgtct gatcctgaag acccgcctca tagccttgca gaatcggcag
      541 gccgtgattc cccgccgcct cgacagtcac atcgccgctg atatcgaggc cgctgcccgg
      601 cgcgccgtaa agatagcgtg cattcgcatc gaccgtactt gagtcaccgg cccgcgcctg
      661 cgtctcttgt ggcttgagtt tgaagtcgag ccgttcgggc acgtaatcct cgacgaggaa
      721 tgtcgcctcg ccgaccggcg cgcctttcgg atcgacgtag gcctcaacac gccatgtgcc
      781 gctggcgctg gatggcaaaa gtgtcagccc gtagctgcgc ccgccagcac cctgatcggc
      841 gatcagaacg cgcttgtatt cgacgccgtc cggccgcttg gcgacaagcg tcagcggcac
      901 gccggtcttg gcactgccct tggcgtcgcg cagcaatgcg gtgacataga cggtctcgct
      961 ggagcgataa acgccgcgct cggtaaaaac ctcagcgtcg agcggctgcg tcacgtcgcg
     1021 gccggagacg ccgcgatcgg tcagatcgaa cgcgccctgc tgcatattga ggaagccgta
     1081 atcgcctttg ccgtcctcac cgacgatgag acccggcgcg aggccattgc tgccgcgcga
     1141 gaggcccggc tcaaagcgaa catagccgtt ggcatcggtc tgcttggtcg cgagcacttc
     1201 gttgtcgcgc gcgacaagac gtagcgaaat gcccgcaagc ggcttcgcgc tcgtcagcga
     1261 gcgcacgaag acatgcagac cgtcgcggct caaaagcgca gtgaggccca gatcggacac
     1321 gacgaaccat tgcgtcgcga cattcgaatc gtcgtcgcta tccgaagact ggagcccgtc
     1381 gccgggcttc gccgtcatga tgtaaacgcc cgcctgcaag gcgccggccg cttcaagcac
     1441 aggaaaggcg gtcgtcacat ccttgttcag caggctcgcg acatcgagcg tacccttcca
     1501 gattttgcgc gcatcgctgt tcttaagctg atcgagccga tagctcgaaa gctgggagag
     1561 aaaatcgctg gacgtgatga tcggcagcag gccgcggtcg ccgacgcgca gaatttgcac
     1621 cgcaatcttt ttgacgttga cggagacaac cggaatgccc tgctgaccga cgcgcggcag
     1681 cacataattc ttgccggtaa aatgtacttg ttgcgaccgg tcgcgcacat agatgttgta
     1741 ggtcgccgtg cgcaaaagct gctcattgac cgccgaagga atcccctgcc gcagaacgat
     1801 cgtgtaatgc gccccgtgct gcaggccatc gacgcaaatc tgctgatctt ccgccgaaac
     1861 cgccggtcga tcgataccgg cgacgctcac ataggacgcg aaatagacgt ggccgccggc
     1921 cagcgactcg gagagctgga agcacgcccg cggcgcggcg gaatccttat cgaccttgta
     1981 gtcgaggatg cggaagccat aggtgtcgcg ctctttttga taggtcgtcc gcaccgccag
     2041 atcgtccaca agcccaaggc ttgcgtgata ggcgtcgagc gaagggcgcc acgattcacg
     2101 ctgcgcaaag atggcgccga gctgcgcgag cgcgagggcg gcttgccgtt tgtcggcggc
     2161 cttgaggtaa gccagatagg cggccgtcgt tgccgcttgc agaatgtcgt cggaattgcc
     2221 ggacgcaact tccgcgcgcg aataggcaag ccagcttgtg gcatcgttcg gattaatggc
     2281 gatcagcccg gccagagcgt gaagcatacc cttcgaatcg cgcttggcga gcgccgcagc
     2341 cgcggcctgc cgaagtgcgt ccggcgtctg atccgtcaga tcatcgccgt cctgcgccac
     2401 ttgcgccgcg agccgaacgg catcgctggc gagatcggcg tttcgataat cgctttgcag
     2461 agtctcggcg cggccggaga cggcgagaac cagcagcaga cacagaaagc cagcgaccgc
     2521 gcgcatattc gctccgtccg ttaaaaaggc gccggttttc cgccgcaggc gcgcccaagc
     2581 tgccgcatcg ccaagggatg gcaaaataat gtggtgattt cgcaacggcc ggcccgaatt
     2641 ggcaaggact ggcttggcct ctgacacaaa ataattgaac ccgtctgcta tgagctatgc
     2701 taaaggctcg ctttatcaaa tagttaacat tccagctcga gggcacaagt ggatgcgacg
     2761 caacgcttga cctgcccgcg gcgcgactca tctacagaaa gggcaacggg acttgcccct
     2821 ggcgcgttca gcccacataa cccgcctgcc agagcgcttg cacgacggaa aatgaatggc
     2881 gatcaatgag gaagtacgcc ccgaacgcat gaagggccgg cgcggcctgg ggggcagtct
     2941 gccgcagaag cctaatctac actcgtggct ggcgagcttt tccgcggagg gcggcggcga
     3001 agaccacaag cgccgacgac agatcgcgac ctacattggc gcgtgtttca gtctgggcat
     3061 cgcggccctt gccattttcg tcctcatcca caccctggcg catatcaacc ccggccaatt
     3121 gcgggccgcg ttcgatgcca ccgaaaacaa ccagatcctc gccgctctcg gcctgacggc
     3181 tttatctttc ctcgcattga ccggctacga cggcgtcgca ctgctgcaat tgcgccagaa
     3241 agtgccgtat acgacgacgg cgctcgcctc gttcacgagc tatgcgattt gcttcacgct
     3301 cggctttccg ctgattaccg ccggcacggt gcgcttctgg atttatt
//
LOCUS       DASWOG010000617         5670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_180042,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000617 DASWOG010000000
VERSION     DASWOG010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180042"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..687
                     /locus_tag="VGB93_12665"
     CDS             121..687
                     /locus_tag="VGB93_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0146969.1"
                     /translation="MPRLTPIPPADLSAEQRPLYDDMKEGIEKNFKGFTAIREDGALI
                     GPWTAWLAEPGFGKASWDLVRALADKPVLPRNVREVAILVTGAHFKSAYELYAHVLVG
                     EMRGLSDEKISTIVAGQRPVDLTREEGVAYDFASALVNGGTLPELTYHTAQKIFTPHG
                     AAELSYLVGLYALVSITLNTFDIPVPEV"
     gene            complement(695..1354)
                     /locus_tag="VGB93_12670"
     CDS             complement(695..1354)
                     /locus_tag="VGB93_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0146970.1"
                     /translation="MSVVQTFKIGELTVAPGASILLEQARASHLFTVLSGWAFRYKTL
                     EDGRRQIINYALPGDFLGLQAAMSDMMGHGIEALTDTVLCVFPRDKLWEFFAADTQLA
                     YNMTWLAASQERSLDEQILSLGRRSAIERVAYLLWYLFDKARELGMTKRNRLVMPMRQ
                     SHLADTLGLSVVHTNKTLQKLRKAGSIELDDRTLRIVDEQALKTLARVDEVQNPPRPL
                     I"
     gene            1628..2635
                     /locus_tag="VGB93_12675"
     CDS             1628..2635
                     /locus_tag="VGB93_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEY0146971.1"
                     /translation="MRVLDLIVGTDQKGFWAVRTYLVALGAVVVALCLQYISWTVLHP
                     FPLLFYFPAVCLAALYGGAGPGALAAILSGLCVFFFAWPGVDGVRHWAISGPFAAYAI
                     VATICIFMFDAARNAIVDLRQQNDEAATQLREADGRIKALRHQMTNNMQAVASLLTLQ
                     KMKLKTDPWAAASLLDDARQRVVDMSRISRRLSERAAAAMGFRQYLQLLCADLQYEAV
                     VSSHEIVGTVSDDVDITDAEKLMAVSVLIGEAVSNALKHAFRENQGGTISVEMKRIAA
                     DQCQLIVQDNGRGLPEGLDPAAAKTGGFLVMQAMAVQLGGKLEHLPSPQGTTLIVIFE
                     I"
     gene            complement(2694..3386)
                     /locus_tag="VGB93_12680"
     CDS             complement(2694..3386)
                     /locus_tag="VGB93_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0146972.1"
                     /translation="MPEVIFNGPAGRLEGRFHPSTQRGAPIAIILHPHPQFGGTMNNQ
                     IVYNLYYAFAERGFSVLRFNFRGVGRSQGAFDHGQGELSDAAAALDWAQTINPDARAC
                     WIAGVSFGAWIGMQLLMRRPEVEGFISVAPPANRFDFSFLAPCPSSGLFVHGDQDRVA
                     PLKEVTGLIEKLKTQRGITIEHAVIPGANHFFENCVEPLIEEVSIYLDRRLGNPPKLP
                     TPTRAEPGDRPS"
     gene            3660..4838
                     /locus_tag="VGB93_12685"
     CDS             3660..4838
                     /locus_tag="VGB93_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY0146973.1"
                     /translation="MPLRRAYLDHNATTVLRPAAREAMLAALTQTGNASSVHREGRAA
                     RARLEAARETVAHFVKTQPKNIYFTSGATEAANLVLTPELELDGNKTPFDVLLISAGE
                     HACVLQGHRFPAAKVEKLPLTPQGVLDLGALEAALARHAAGTRVLLALQAANNETGVL
                     QPVAEAASRVHAVGGALVCDAVQAAGRSDCDFATLGADVLVLSAHKIGGPQGVGALCF
                     ARDRHHIVPTIVRGGGQERGLRAGTENVAAIVGFAASVAGVTEAGPREMEHLAALRDR
                     LERDVLASAPDAVFFGAAAQRLPNTTNFAVPGLSAEVLLMALDLEGVAISSGSACSSG
                     KVKRSHVLEAMSVPSALADGAIRVSSGWSSEEDDVDMFRTAFSKAVETIRAKRVKPAA
                     "
     gene            4891..>5670
                     /locus_tag="VGB93_12690"
     CDS             4891..>5670
                     /locus_tag="VGB93_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0146974.1"
                     /translation="MAAVQETIDRVKSIDVSEYKYGFFSDIESDKAPKGLSEDIVRFI
                     SAKKNEPEWLTEWRLEAYRRWLTMQEPRWARVSYEPIHYQDLYYYAAPKTASGPKSLD
                     EVDPELLKIYEKLGIPLNERAILAGVEGAGEASSRVAVDAVFDSVSVVTTFKEELGKA
                     GVVFCSISEAVQTHPKLVRKYLGSVVPPTDNFFATLNSAVYSDGSFVYIPPGVRCPME
                     LSTYFRINEKNTGQFERTLIIADKGSYVSYLEGCTAPKRDEN"
BASE COUNT         1080 a         1633 c         1779 g         1178 t
ORIGIN      
        1 ggttcacgcc gaattagcac gttccggctg ggggccagac ccgccgccac ataaataata
       61 cgagccgcgc ctacattggc ttcggccata tccggccacg atctgatgcg agggactgca
      121 atgccacgcc tgacgcccat cccgcctgcc gacctgagcg ccgagcaacg cccgctctac
      181 gacgacatga aggaaggtat cgagaagaat tttaagggct tcaccgccat tcgcgaagat
      241 ggcgcgctca tcgggccgtg gacggcgtgg cttgccgagc cgggattcgg caaggcgagc
      301 tgggaccttg tccgtgctct ggccgacaag ccggtgctgc cgcgcaatgt ccgtgaggtc
      361 gccattcttg ttaccggcgc gcatttcaaa tcggcctacg aactctatgc gcatgtgctc
      421 gttggcgaga tgcgcgggct ttcagacgag aaaatctcga cgatcgtcgc cggccaacgc
      481 ccggtcgatc tgactcgcga ggaaggggtc gcctatgatt tcgccagcgc cctcgtcaat
      541 ggcggcacat tgccggagct gacctaccac acggcgcaga agatctttac gccgcatggc
      601 gcggcagaac tctcgtatct tgtcggcctc tatgcactcg tctcgatcac gctcaacacg
      661 ttcgatattc cggtgccgga agtctgatct gcgcttagat cagcgggcgc gggggattct
      721 ggacttcgtc cacgcgggcc aatgtcttca acgcctgctc atcgacaatc cggagggttc
      781 ggtcgtcaag ttcgatcgaa cccgccttgc gcagcttttg cagcgtcttg ttggtgtgga
      841 cgaccgaaag accaagcgta tcggcaagat ggctctgccg catgggcatg acgagccggt
      901 tgcgtttcgt catgcccagc tcacgcgcct tatcgaaaag ataccacaag agataggcga
      961 cgcgttcgat cgcgcttcga cggccgagcg atagaatctg ttcgtcgagc gagcgctcct
     1021 ggctcgctgc aagccatgtc atgttgtagg cgagttgcgt atcggcggcg aagaattccc
     1081 aaagtttgtc gcgcgggaaa acgcagagga ccgtatccgt cagggcctcg attccatggc
     1141 ccatcatgtc cgacatcgcg gcctggaggc caagaaaatc tcccggcagc gcgtaattga
     1201 tgatttgccg gcgcccatct tccagcgtct tgtagcgaaa ggcccagccc gagagcacgg
     1261 tgaaaagatg gctcgcgcgg gcttgttcaa gcagtataga agcgccgggc gcgacggtga
     1321 gttcgccgat cttgaatgtt tgtaccacac tcacgtcgat gagctctccg gcgaaaacgg
     1381 gcaacggccg caaagggcaa ttcgcgcacg ccgttgtcat cgcagtttgg ctcaggccgc
     1441 cgcttgtcat tagtcactcg ttatcaaaga taatgtcgcc cgtcggcccg gtgtatttgg
     1501 gccgtttata caaggcgacg aaattgcgcg aggcgcaagc ccttctcgtt cagggcatta
     1561 tgtcaaagtt aaatgaccgt tcgccacgtt gaggtctatg cgccagccag gcggggcgcg
     1621 actcaagatg cgagttttgg atcttattgt tggtacggac cagaaaggtt tctgggccgt
     1681 tcgtacttat cttgtcgccc tcggggccgt cgttgttgcc ttgtgcctcc agtatatatc
     1741 ctggacggtg ctgcaccctt tcccgttgct gttttatttc ccggcggttt gccttgcggc
     1801 gctctatggt ggcgcggggc ctggggccct tgcggcgatt ctttcaggcc tgtgcgtctt
     1861 ctttttcgcg tggccgggcg ttgatggtgt ccgccattgg gcgatctccg ggccgtttgc
     1921 ggcttatgcg atcgtcgcga cgatctgcat cttcatgttc gatgctgccc ggaatgcgat
     1981 tgttgatctg cggcagcaaa atgatgaggc ggctacgcaa ttgcgtgaag cggacggtcg
     2041 gataaaagcg ttacgccacc agatgaccaa taacatgcag gccgtggcga gccttctgac
     2101 cttgcagaaa atgaagctga agacggatcc ctgggcggcg gcgagtttgc ttgacgacgc
     2161 gcgtcaaagg gtcgtcgaca tgagccgtat cagccgccgc ctcagtgagc gcgcggctgc
     2221 ggccatgggc ttccgccaat atctgcaatt actatgcgcc gatctgcaat acgaagccgt
     2281 tgtgtcgtcg catgaaatcg tcggtacggt ttctgatgat gtcgatatca ccgacgcgga
     2341 aaaattgatg gccgtatctg tcttgatcgg cgaggccgtc agcaatgcgc tcaaacacgc
     2401 atttcgcgaa aatcaaggcg gcaccatttc cgtcgagatg aaacggatcg ctgccgatca
     2461 atgtcagttg atcgttcaag acaatggccg cggcttgcca gagggcctcg accccgcggc
     2521 ggcaaagacg ggcgggttcc tcgtcatgca ggccatggcc gtacagctcg gcggcaagct
     2581 cgaacacttg ccgtcgccgc agggcacgac cttgatcgtg attttcgaaa tctaagcttt
     2641 tgatcgcatc ggatttgccc gaaaaaccgc tacgcacttt tcggtccgat gctttagctc
     2701 ggccggtcgc cgggctcggc ccgggtcggc gtcggcaatt tcggcggatt gccgaggcgg
     2761 cggtcgaggt agatcgaaac ttcctcgatc aatggctcga cgcaattttc gaagaaatga
     2821 ttggcaccgg ggatgaccgc gtgctcgatc gtgatgccgc gctgcgtttt cagcttctcg
     2881 atgaggccgg tcacttcctt cagcggcgcg acgcgatcct gatcgccgtg gacgaacagg
     2941 cccgaggacg ggcagggcgc gaggaaggaa aaatcgaacc ggttggccgg cggggcgacg
     3001 gagatgaagc cttcaacctc cggccggcgc atcagaagct gcatgccgat ccaggcgccg
     3061 aaggagacgc cggcaatcca gcaggcgcgc gcatcgggat tgatcgtctg cgcccaatcg
     3121 agcgccgcag ccgcgtccga aagctcccct tggccgtgat cgaaagcgcc ctggcttcgg
     3181 ccgacgccgc ggaagttgaa tcgcagcacc gagaagccgc gttcggcaaa ggcgtaatag
     3241 aggttataga cgatctgatt gttcatcgtc ccgccgaatt gcgggtgggg atgcagaatg
     3301 atcgcgatgg gcgcgccgcg ctgcgtcgag ggatgaaaac gtccctcaag acgaccggcg
     3361 gggccattga aaataacctc gggcattccg tctcctaaag cttctggtgg actaggccca
     3421 agtgcggcaa ctcgcctgca tatctgagct tttccgtggg tttgcttgac gaacgagcca
     3481 ccgaccacat aatctggagt tagaactgtt ctaaccgttt cggcaccttg cagcgccagc
     3541 acgtcggctt gtcggcaaat caggggcttc atctacacgg ccagcagtgc gaattgcaat
     3601 tattttggct tggcggtgtg cttttttgat tcgccccccg gcctgagaag gaatgaacgg
     3661 tgccgctgcg acgcgcctat ctcgaccaca acgctacgac cgtgctgcgt cccgccgcgc
     3721 gcgaggctat gctggccgcg ttgacgcaga ccggcaatgc ctcgtccgtc caccgcgagg
     3781 gccgtgcggc gcgggcaagg ctggaggcgg cgcgcgagac cgtcgctcat ttcgtcaaga
     3841 cgcagccaaa gaacatctat ttcacctctg gcgcgaccga ggcggcaaac ctggtcctga
     3901 cgccagagct tgagcttgac ggcaacaaaa cgccgttcga cgtgcttttg atctcagccg
     3961 gcgagcacgc ctgtgtcctt cagggccacc gttttccggc ggcgaaagtc gagaagctgc
     4021 cgctgacacc ccagggcgtg cttgatcttg gcgcgctcga agctgccttg gcacgccatg
     4081 ccgccggcac ccgtgtgctg ctggcattac aggctgccaa caacgagaca ggcgtgctac
     4141 agccggtcgc tgaggccgcc agtcgcgtcc atgccgttgg cggggcgctt gtctgcgacg
     4201 cggttcaggc agcgggcagg tctgactgtg actttgccac tctgggcgct gacgttctgg
     4261 tgctttcagc gcataaaatc ggtggcccgc aaggggtggg cgccctttgt tttgcaaggg
     4321 atcgtcatca tattgtgcct acgatcgttc gcggcggtgg ccaggagcgc gggttgcgtg
     4381 ccggtacgga gaacgtcgca gcgattgttg gttttgccgc atctgttgcg ggcgtgacgg
     4441 aagccggacc gcgtgagatg gaacatttgg cggccttgcg tgaccggctg gaacgtgacg
     4501 ttctggcgag cgcgccggac gcggtgtttt tcggggcggc cgctcaaagg ctgccgaaca
     4561 cgacgaattt tgcggtgccg ggtctgagcg ccgaagtttt gctgatggcg ctcgatctgg
     4621 aaggcgtggc gatctcgtcc gggtccgcct gctcttcggg caaggtcaag cgctcgcatg
     4681 tgcttgaggc gatgagcgtg ccatccgcgc tggcggacgg ggcgatccgg gtgagctccg
     4741 gctggagttc cgaggaggat gatgtcgata tgtttcgtac cgctttcagc aaggcggtcg
     4801 agacgatccg ggcaaagcgg gtgaaacccg ccgcatagag attgaagtct gaaccagcgg
     4861 tccttgaaac cgccgggtga ggagagaaga atggctgcgg tgcaggaaac gatcgatcgt
     4921 gtcaaatcga tcgatgtgag cgaatataag tatggcttct tctctgatat tgaatcggac
     4981 aaggcgccga agggattaag cgaagatatc gtccgcttca tttcggcgaa gaagaacgag
     5041 ccggaatggt tgaccgaatg gcggctcgaa gcctatcggc gttggctgac gatgcaggag
     5101 ccgcgctggg cgcgtgtatc ctatgagccg atccattatc aggatctcta ttattacgcc
     5161 gcgccgaaga cagcgtcggg gccgaagtcg cttgacgagg tcgatccgga actcctgaag
     5221 atttatgaga agctcggcat tccgctgaac gagcgggcga tccttgccgg cgtcgagggt
     5281 gccggcgaag catccagccg tgtcgctgtc gatgcggtgt tcgattccgt ctcggtcgtc
     5341 acgaccttca aggaagaact cggcaaggcg ggcgtcgtct tctgctcgat ctcggaagcg
     5401 gtgcagacgc atcccaaact cgtgcgcaaa tacctcggct ccgtcgtgcc gccgaccgat
     5461 aatttcttcg caacgctgaa ctccgcggtc tattcggacg gctcgttcgt ctacatcccg
     5521 ccgggtgtcc gctgcccgat ggaactctcg acctatttcc gcatcaacga gaagaacacc
     5581 ggccagttcg agcgcacgct gatcatcgcc gacaagggct cttacgtctc ctatctcgaa
     5641 ggctgcacgg cgccgaagcg cgacgagaac
//
LOCUS       DASWOG010000618         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_272342,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000618 DASWOG010000000
VERSION     DASWOG010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272342"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1173)
                     /locus_tag="VGB93_12695"
     CDS             complement(190..1173)
                     /locus_tag="VGB93_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0146975.1"
                     /translation="MTLPTVLMISLGGTITMTRNASGGIVPTLDANDLVRTVPGLDNV
                     AQIETLSPFRKPGASLTFDDLMAVAMLINAQLASGIAGAVVIQGTDTIEETAFVLDRL
                     VRGDAPVVVTGAMRGAEAAGADGPANLLASVITAASPGARGRGVLVVLNDEIHAARFV
                     QKSHTALPSAFRSPLCGSVGLVIEGRALFQLDPRRTPSLDGEWSNIDRPVALLKMGLG
                     DDGRLITALPSLGFAGAVIEGMGAGHVPADIAPLLGTIAEQMPVILASRVETGPSFTQ
                     TYAFPGSEVDLLGRSLISGGSLSGLKARLLLSLLLRTGHDRLQIRAAFEMN"
     gene            complement(1183..2073)
                     /locus_tag="VGB93_12700"
     CDS             complement(1183..2073)
                     /locus_tag="VGB93_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0146976.1"
                     /translation="MTDMIGSSVRGRLPYTPIHRRPKLKLPGDARVAVWTIVNVENWS
                     PSGAMPRAVLPPPMGQPLLPDVPNWAWHEYGMRVGFWRFVETLDARNLKATFAINGSV
                     CDLYPDACEAARDAGWDFMGHGFVQKPMHKVEDQKSAIAETIEAIQKFTGKPPRGWES
                     PGLTETDQTLDLLAEAGIEYVADWVLDDQPVMLKTTAGNIVSVPYTVEINDVVISAVH
                     QQPSDEIYRRGKDQFDRLYLDGAKAPRVMAISIHPYLTGVPHRIKYLEMLYDYILGHD
                     GVVMWTGAEILDWYRAQIIE"
     gene            complement(2090..3079)
                     /locus_tag="VGB93_12705"
     CDS             complement(2090..3079)
                     /locus_tag="VGB93_12705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0146977.1"
                     /translation="MHIDIHSHVIPQRVVTAIEADPKQFQARIEGDGAARKMVHAQGY
                     VYPLSEEFYHAEAKLEAMDRKGIDISMISPAPPMFCYWADADAALELAALVNDGVADM
                     VATKPSRLRGMATVPMQHPDAAVAELERVVNAYNFKAVEIGTSIEGAQLAEERFRPLL
                     RRASELGVFVFAHPYYVSAKAGLEAYYLTNLIGNPLDTTVMLTNLMFSGRLDELPDLK
                     LVLAHGGGFSPYQIGRLVHGHKVRAEAKKITKSSPKDLLRQVYFDSLVFEPQALRYLI
                     DLVGADHVCIGSDAPFDMADDNPMATIECVPRINEQECACVRCGTALKLLRET"
     gene            complement(3079..4581)
                     /locus_tag="VGB93_12710"
     CDS             complement(3079..4581)
                     /locus_tag="VGB93_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY0146978.1"
                     /translation="MLNQDAPFGLYINGQFRPSSTGGTIPVENPKDGRIIAHVAEGEA
                     ADVDAAIDAAEEVGKIWAATPGAVRGDIMHRAAELLAGRLNDLVTIEVDQIGRPRREM
                     SAQLGRLPEWFRYFGAIARTHEDTVPPFGGNFLNYTRRVPLGVVGHVTPWNHPLLILT
                     KKVAPAMAAGNTMVVKPSELAPITPLLLGDIFKEAGVPDGVYNVVPGYGATAGLALSS
                     SSRIAKIDLTGGTETGRAVAAVAGHNLTRVAAELGGKASVVVFPDTPIDRSVAATLFA
                     SFIAAGQTCVQGARLVVHRSIHDEVVEKLVARANAIKIGDPQDLATQMGPLVSAKQRD
                     LTERYVKIGLEEGATLATGGRRPEGSAFRNGYYYTPTIFTGVTNDMRIAQEEIFGPVV
                     CVIPFETEEEAIALANGTEFGLATSIWTRDLARAHRVAHRLQSGITWINDHHRIDPCS
                     PWGGFKMSGLGRENGLVAYEEYTQIQNIIVNLSDETFDWYAQDGVEKRYS"
     gene            complement(4605..5309)
                     /locus_tag="VGB93_12715"
     CDS             complement(4605..5309)
                     /locus_tag="VGB93_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0146979.1"
                     /translation="MLEIRDLQSFYGDAHVVQGASLTVRNHEVVALLGRNGMGKTSLI
                     RSVMGLSAPRVRGGNVTWKGEDLTGLKPHDIARRKIALVPQGRRLFPSLTVIEHLTMI
                     KPIRIKEGWTAERIFELFPRLAERRNNRGNQLSGGERQMLAVGRALMIDPELILMDEP
                     SEGLAPVMVQHLEDIIVQLKHDGIAILLVEQNLYSALAVADRAYVLETGKVVYETQAS
                     ELVDDPRSLVRFLGVH"
     gene            complement(5302..>5553)
                     /locus_tag="VGB93_12720"
     CDS             complement(5302..>5553)
                     /locus_tag="VGB93_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0146980.1"
                     /translation="LLLDEPAAGLSPAERVIVTDVIRALPADITIVLIEHDMDLVLSL
                     VDWITCLNNGQFLADGAPADIRENADIQNVYLGRPRHHA"
BASE COUNT         1140 a         1691 c         1604 g         1118 t
ORIGIN      
        1 catcgttcaa agccgggtga gctattgaaa tatttggcgc tccagatccg acatggaagc
       61 aagccttccg gacgaccgga cttggctgat gattcagccg atatgcgggc tactaaacct
      121 ccggcgcatc gacctctgga ggctgtttaa ggcttggcgc aatccatatc ttgggcgacg
      181 gattggcctt cagttcatct cgaacgccgc acgaatttgc aggcgatcat ggcccgtacg
      241 aagaagcagg ctgagcaaca gtctcgcttt tagcccactt aaagacccgc ccgatatcaa
      301 gctacgccca aggagatcga cctccgagcc gggaaaagca taggtttggg tgaacgaagg
      361 cccggtctcc acacgcgagg caaggatcac gggcatttgc tcggcaattg ttcccagcag
      421 cggtgcaata tcagcgggca catggcctgc gcccattcct tcgatcacgg cgcccgcgaa
      481 acccagcgat ggcaaggctg tgataagtcg cccatcatca ccgagtccca ttttgaggag
      541 cgcaaccggg cgatcgatgt tcgaccattc cccatcaagc gacggggtgc gccgcgggtc
      601 gagttgaaat agtgcgcgcc cttctattac cagacccacc gaaccgcaga ggggcgaacg
      661 aaatgccgaa gggagcgccg tatgactctt ctgtacgaac cgcgccgcgt gaatttcatc
      721 gttcaggaca accagaacgc cgcggccgcg cgcacccgga ctggccgccg tgatcaccga
      781 tgcaagcaaa ttcgccggac cgtcagcacc agcagcctcg gcgccacgca tagcgcccgt
      841 gacgacgaca ggcgcatccc ctctgacgag gcgatcgagc acgaaggcgg tctcctcgat
      901 cgtgtcggtt ccctgaatga cgaccgcgcc agcgatgccg ctcgcaagct gcgcgttgat
      961 cagcatagcg acagccataa gatcgtcgaa cgtcagcgat gcaccgggct tgcgaaacgg
     1021 cgacagcgtc tcaatttgcg ccacattatc gaggcccggc accgtgcgta cgagatcatt
     1081 cgcatcgagc gtcggcacga tcccgccgct cgcgtttcgc gtcatggtga tggtcccgcc
     1141 gagcgagatc atcaaaacgg ttggtaatgt catagccgcc ttttactcaa ttatctgcgc
     1201 gcgataccag tcaaggattt ctgcgccggt ccacatcacc accccgtcgt gaccgagaat
     1261 atagtcatac agcatttcga gatatttgat ccgatgcggc acaccggtga ggtaaggatg
     1321 gatcgagatc gccatgactc gcggcgcttt ggctccatcc agatacagcc gatcgaattg
     1381 gtctttgcca cggcgataga tttcgtcgga cggctgctgg tgaacggcgc tgatgacaac
     1441 atcattgatt tcgacagtat acggtacaga cacgatattt ccggcggtcg ttttcagcat
     1501 aaccggctga tcatcaagca cccagtcggc cacatattca atgccggctt ctgcgaggag
     1561 atcgagcgtc tggtctgtct cagtcaggcc agggctttcc cagccacgcg gcggcttacc
     1621 agtaaatttt tggatggctt cgatcgtctc ggcaatcgcc gatttctgat cctctacctt
     1681 gtgcatcggc ttttgcacga agccgtgacc catgaaatcc cagcccgcgt cgcgtgccgc
     1741 ctcgcacgcg tcaggataaa gatcgcagac cgacccgttg atcgcgaagg tcgccttcag
     1801 gttacgcgcg tcaagcgttt ccacgaaccg ccagaatccg acgcgcatac catattcgtg
     1861 ccatgcccaa ttcggaacgt cgggcaacag cggctgcccc atcggcggcg gcaagaccgc
     1921 gcgcggcatc gcgccgctcg ggctccagtt ctcgacattg acgatcgtcc agaccgccac
     1981 gcgggcgtcc cccggcagtt taagcttggg acggcggtga attggtgtgt aaggcagccg
     2041 gccgcgaacg cttgagccga tcatatcggt catttcttta ctcctgcact caggtttcac
     2101 gcagcagctt cagcgccgtg ccgcagcgta cgcacgcgca ttcctgttca tttatgcgcg
     2161 gcacgcattc aatcgtcgcc attgggttgt cgtcggccat atcgaatggg gcatcactcc
     2221 cgatgcagac atgatccgcg ccgaccagat cgatgaggta gcgcaacgct tgcggctcga
     2281 acacgaggct gtcgaagtaa acctgcctca acagatcctt cggtgacgac tttgttatct
     2341 tttttgcttc ggcacgaact ttatgaccgt ggaccagacg gccgatctga tagggcgaga
     2401 aacctccgcc atgcgcaagc acgagcttca ggtcgggcaa ttcgtcgagc cgcccggaaa
     2461 acatcagatt ggtgagcatg actgttgtgt cgagcggatt gccgatcagg ttggtgagat
     2521 aataggcttc gagcccggct ttggcgctga cgtaataagg atgcgcaaag acgaacacgc
     2581 caagctccga cgcacgccgc aagagcggtc ggaaccgctc ttctgccagt tgagcgcctt
     2641 cgatcgatgt gccgatctcg acagccttga agttataggc gttgaccacg cgctccagtt
     2701 cggcgaccgc agcgtccgga tgctgcatcg gaacggtcgc catgccgcgg agccggctcg
     2761 gctttgtggc aaccatgtca gcgacgccat cgttgacaag agccgcaagt tccagcgcgg
     2821 cgtctgcgtc cgcccagtag cagaacatgg gcggtgctgg agagatcatg gaaatatcga
     2881 tgcccttgcg atccatcgct tcgagcttcg cctcggcgtg ataaaactcc tcagagagcg
     2941 gatagacata gccctgcgcg tgaaccatct tgcgcgcggc cccatcaccc tcgatccgag
     3001 cctgaaactg cttcggatcg gcctcaattg ccgtgacgac acgctgggga atgacgtggc
     3061 tgtggatatc gatatgcatt agctgtagcg cttttccacg ccgtcctggg cgtaccagtc
     3121 gaacgtttca tcggacagat tcacaatgat gttttggatc tgcgtgtatt cctcgtaggc
     3181 gaccagaccg ttctcgcgtc cgagtccgct catcttgaat ccgccccatg gcgagcacgg
     3241 atcgatccgg tgatgatcgt tgatccaggt aatgccgctc tgcaggcggt gggcgacgcg
     3301 gtgcgcgcgg gccagatcgc gtgtccagat cgaagtcgcg agaccgaatt ctgtgccatt
     3361 tgcgagcgcg atcgcctctt cctccgtttc aaacggaatg acgcagacca ccggcccgaa
     3421 gatctcttcc tgcgcgatgc gcatgtcatt cgtcacgccg gtaaaaatcg tgggcgtgta
     3481 gtaataaccg ttgcggaagg cgctgccctc cgggcgcctg ccgccggtgg ccaaggtcgc
     3541 accctcctcc aggccaatct tcacatagcg ttcggtgaga tcgcgctgtt tggcagagac
     3601 gagcggcccc atctgagtgg ccaagtcttg cggatcaccg attttgatgg cgttggcgcg
     3661 cgccacaagc ttttcgacca cctcgtcgtg aatcgaacga tgcacgacga gccgcgcgcc
     3721 ctgcacacag gtttggccag ctgcgatgaa cgatgcaaac aatgttgccg cgacactgcg
     3781 gtcgatcggc gtatccggaa aaaccaccac ggacgctttg ccgccgagct ccgccgccac
     3841 ccgcgtcaga ttatggcccg cgaccgcggc caccgctctg ccggtctccg tgccgcccgt
     3901 gagatcgatc ttcgcgatac gtgaactcga cgacaatgca agaccggccg ttgcgccata
     3961 accgggcacg acattgtaga ccccgtcggg aactcctgct tccttgaaga tgtcaccgag
     4021 aagcaacggt gtgatcggtg caagttcgct cggcttcacg accatggtgt tgccagccgc
     4081 catggctggc gcgactttct ttgtcaggat cagtagcgga tgattccacg gcgtgacatg
     4141 gccgacgacg ccgagcggca cgcgtcgcgt ataattcagg aagttcccac cgaacggcgg
     4201 aacagtatcc tcgtgcgtgc gcgcaatcgc gccaaaatag cgaaaccact cgggcagacg
     4261 gcccaattgg gcgctcatct cccggcgcgg tcgcccgatt tggtcaacct cgatcgtcac
     4321 cagatcattg aggcggccgg ccagaagctc ggcggcgcga tgcatgatat cgccgcgcac
     4381 ggcaccgggg gtcgcggccc agatcttccc cacctcttca gcggcgtcga tggccgcatc
     4441 gacatccgcc gcttcacctt ctgcgacatg cgcaatgatg cggccgtcct tcggattctc
     4501 gaccgggatt gtaccgccag tcgaacttgg tctgaattgg ccattgatat agagcccgaa
     4561 cggagcgtcc tgattgagca tgtttcgtcc tgacattgat ggaattaatg cacgccgaga
     4621 aagcggacga gtgaccgcgg atcatcgacg agttcggagg cctgggtttc gtagaccacc
     4681 ttgccggtct caagcacata cgcgcgatcg gcgaccgcaa gggcgctgta aagattttgt
     4741 tcgaccagaa ggatcgctat gccgtcatgt ttgagttgca cgatgatgtc ctcaagatgt
     4801 tgcaccatga ccggcgcgag cccttccgat ggctcatcca tcaagatcag ttccggatcg
     4861 atcatcagcg cgcgcccgac ggcgagcatt tgacgctctc cgccagacaa ttgattgccc
     4921 cgattgttgc ggcgttctgc gaggcgtgga aacaattcga agatgcgttc ggccgtccac
     4981 ccttctttga tgcggatcgg cttgatcatt gtcaggtgtt caatgacggt gagcgaagga
     5041 aatagccgcc gtccctgagg cacaagcgcg atctttcgcc gggcgatatc gtgcggcttg
     5101 agcccggtca gatcttcacc tttccaggtg acattgccac cgcgcacgcg tggcgcggaa
     5161 aggcccatta ccgagcggat gaggctggtc ttgcccatcc cgttgcggcc gagcagcgcc
     5221 acgacttcgt gattgcggac ggtgagcgag gctccctgaa cgacatgtgc gtcaccataa
     5281 aaactttgaa gatcgcgaat ctcaagcatg atgacgaggc ctgccgaggt agacgttctg
     5341 gatatccgca ttctcccgga tatcggcggg cgcgccgtcc gcaaggaact gtccattgtt
     5401 caggcatgtg atccaatcga ccaatgacaa caccagatcc atgtcatgct caatcagcac
     5461 gattgtgatg tccgcgggca aggcgcggat gacatcggtg acgatcacgc gctcggcggg
     5521 cgacaggcca gcggcgggct cgtccagcag cag
//
LOCUS       DASWOG010000619         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_272349,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000619 DASWOG010000000
VERSION     DASWOG010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272349"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGB93_12725"
     CDS             <1..567
                     /locus_tag="VGB93_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HEY0146981.1"
                     /translation="PAMAGMIYAMPGMETQLHAVMTQTGTYAGLSANYSGDGFAGMHF
                     PYTSLSVADFDKWVAKVKSDGGALDRNTYLDLERPSQNLSPKTWGTVDAALFKLALNQ
                     CVEPGKMCLDEMMAIDAKGGLGLAGVNNIMPLVYDKYARRGSGPMRAYVAALCQRNGA
                     PETGASVADLARRISEPAHLASPQASAE"
     gene            592..>1713
                     /locus_tag="VGB93_12730"
     CDS             592..>1713
                     /locus_tag="VGB93_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0146982.1"
                     /translation="MLDPTLQKEIFGRLTLDSIPYHEPILIMTFAGVAVAGVVVLGTL
                     TYLRAWGYLWKEWFTSVDHKKIGVMYLVLAIIMLLRGFADALMMRAQQLLASSGGQGY
                     LPAHHYDQIFTAHGVIMIFFVAMPFITGLINLVMPLQIGARDVSFPFLNSFSFWMTVA
                     GAVLTMISLFIGEYARTGWLAYPPLSEAAYSMDVGVDYYIWGLQIAGIGTLLSGINMI
                     CTIVKMRAPGMTLMRMPIFTWTAFCANILIVAAFPVLTAVLALLTLDRYLGFNFFSND
                     FGGNPMMYVNLIWIWGHPEVYILILPAFGVFSEVTATFCGKSLFGYTSMVYATLVITL
                     LSYLVWLHHFFTMGSGASVNSFFGITTMIISIPTGAKMFN"
BASE COUNT          315 a          541 c          465 g          392 t
ORIGIN      
        1 ccggcgatgg cgggcatgat ctatgccatg ccggggatgg aaacgcagct tcacgccgtg
       61 atgacccaga ccggcaccta tgcgggcctt tcggcgaatt acagcggtga cggcttcgcc
      121 ggaatgcatt tcccctacac cagcctcagc gtggccgatt tcgataaatg ggtcgcgaag
      181 gtcaaatctg acggtggcgc actcgatcgc aacacctatc tcgaccttga acggccaagc
      241 cagaatctgt ctcccaagac gtggggcaca gtcgatgcag cgctcttcaa gctcgcgctc
      301 aatcaatgcg tcgaacccgg caagatgtgc ctcgacgaaa tgatggcgat cgatgccaag
      361 ggcggtctcg gcctcgcggg cgtcaacaac atcatgccac tcgtctacga caaatacgcc
      421 cggcgtggtt cggggccgat gcgcgcctat gtggccgcgc tctgccagcg caacggcgcg
      481 cccgaaaccg gcgccagtgt ggccgatctt gcacgccgga tttccgaacc cgcgcatctt
      541 gcgtccccgc aagcctccgc cgaatgacgc tctcaagcta agacacaggc tatgctcgac
      601 ccgacccttc aaaaagaaat cttcggtcgt ctgaccctcg attcaatccc ctatcatgag
      661 ccgatcctga tcatgacgtt cgccggcgtc gcggtggccg gggtcgtcgt tctcggcacc
      721 ctcacatatc tgcgcgcctg gggctatctt tggaaggaat ggttcaccag cgtcgatcac
      781 aaaaagatcg gcgtcatgta cctcgtgctc gccatcatca tgttgctgcg cggctttgcc
      841 gatgcgctga tgatgcgcgc gcaacaatta ttggcctcca gcggaggcca gggctaccta
      901 ccggcacacc attacgacca gattttcacg gcgcacggcg tcatcatgat cttcttcgtc
      961 gccatgccgt tcatcaccgg gctgatcaat ctggtcatgc cgctgcagat cggcgcgcgc
     1021 gacgtgtcct ttccgttctt gaacagtttc agcttttgga tgacggtcgc cggcgccgtg
     1081 ctgacgatga tctcgctttt catcggtgaa tatgcgcgca ccggctggct cgcctatccg
     1141 ccgctgtcgg aggcggctta cagtatggac gtcggcgttg attattatat ctggggtctg
     1201 cagatcgcag gtatcggaac gctgctctcc ggcatcaata tgatctgtac gatcgtgaaa
     1261 atgcgcgcgc cgggcatgac cctgatgcgc atgccgattt tcacctggac cgcgttttgc
     1321 gccaacatcc tgatcgttgc cgccttcccc gtgctcaccg ccgtgctcgc ccttctcacg
     1381 ctcgaccgtt atctcgggtt caatttcttc tcgaatgatt tcggcggcaa tccgatgatg
     1441 tatgtgaacc tcatctggat ctggggccat cccgaggttt acatcctcat cctgccggct
     1501 ttcggcgtct tctcggaagt aactgcgacg ttctgcggca aaagcctctt cggctataca
     1561 tcgatggtct atgcgacgct cgtgatcacg ctcctttcgt atcttgtctg gctgcatcac
     1621 ttcttcacga tgggctccgg cgcgagcgtg aactcgttct tcggcatcac gacgatgatc
     1681 atctcgatcc ccacgggcgc gaagatgttc aac
//
LOCUS       DASWOG010000620         8005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_161337,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000620 DASWOG010000000
VERSION     DASWOG010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161337"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB93_12735"
     CDS             <1..463
                     /locus_tag="VGB93_12735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0146983.1"
                     /translation="ALAPRMGRAEAACTSPALRAVQTAEALGLAATIDPLLADIDLGD
                     WAGKSLVELEAADAAGLSQWIEDPSAAPHGGESVTHLLTRIASWLANIGAGDDRIIAV
                     THAAVIRAAAIIALDANPKSFWRIDIAPLGFSRFHARGGRWTVHSLNAEAD"
     gene            603..899
                     /locus_tag="VGB93_12740"
     CDS             603..899
                     /locus_tag="VGB93_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146984.1"
                     /translation="MSALGLVVELVAGAIGGNGIASVAKNLNLGTLGNSLVGAIGGAA
                     GTSFLGIIIPALESVGHADIGTLAGQAVGGGVLGAVLTAIVAAIRNASSGAPAA"
     gene            1139..1603
                     /locus_tag="VGB93_12745"
     CDS             1139..1603
                     /locus_tag="VGB93_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0146985.1"
                     /translation="MAQHHPTLGFWLFAGIAATAGVACLLFAIDSSRGDKETVAQSNP
                     ETRCTENWKMCRDHADLVNYSSKADDARHACIAAVNEAGKYGHPKWCSGWLCEDFANY
                     ETGTNAPDDGLITLIDDHVQMQNGSGARIHSTTYCTYNIRTDKVVTLNIGAN"
     gene            1754..3082
                     /locus_tag="VGB93_12750"
                     /pseudo
     CDS             1754..3082
                     /locus_tag="VGB93_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650856.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
     gene            complement(3392..3476)
                     /locus_tag="VGB93_12755"
     tRNA            complement(3392..3476)
                     /locus_tag="VGB93_12755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3440..3442),aa:Leu,seq:gag)
     gene            3639..4358
                     /gene="lipB"
                     /locus_tag="VGB93_12760"
     CDS             3639..4358
                     /gene="lipB"
                     /locus_tag="VGB93_12760"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0146986.1"
                     /translation="MSESLQRQDIVRDLRNPASAPVEWRVLDGLTGYDDALATMEARV
                     AAIAAGKAAEQVLLLEHPPLYTAGTSAAASTDDLLRFPLHKAGRGGQITYHGPGQRVA
                     YVMLDLNRRGPDVRAFVAALEDWIIATLAHFDVHGERREDRVGVWVARPCKPRGPNNE
                     AAEDKIAAIGIRIRRWVTFHGISLNVAPDLSHYSGITPCGIAAPHLDLTSLRDLGVTA
                     TMAEVDEVLRQTFESTFGAGR"
     gene            complement(4361..4981)
                     /locus_tag="VGB93_12765"
     CDS             complement(4361..4981)
                     /locus_tag="VGB93_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0146987.1"
                     /translation="MNLLQIDSSITGPNSVSRVITAAVTERLRQTHPGLHIIERDLIG
                     AAPDHVTPANFPSSHPISAMAGAAPEFAAGRAASDAILDEFLAADIVVIGAPMYNFGV
                     PSQLKAWIDRVIVPGKTFSYGPEGVKGLAAGKRVIIAISRGNLYGSGQPAAAFEHVET
                     YLRSVFSFVGITNLQFIVAEGVQLGPDARQAALDKALQETSVLKAA"
     gene            5071..5454
                     /locus_tag="VGB93_12770"
     CDS             5071..5454
                     /locus_tag="VGB93_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0146988.1"
                     /translation="MDVTIEVPAVEHSDCRRVSQLLARVGDKWSVLIIMTLAAGPKRF
                     SEIKRSVDAISQRMLTLTLRGLERDGLVTRKVFPTIPPKVEYRLSELGCSLCEPVMAL
                     GQWALAHVVEIDTAREKFDRESGAA"
     gene            complement(6179..7030)
                     /locus_tag="VGB93_12775"
     CDS             complement(6179..7030)
                     /locus_tag="VGB93_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0146989.1"
                     /translation="MQIARPSLIALSAVAFFTTLVAAPAVTVAFDGTPQSSTSVDKMP
                     LQIFKDPEAALRAGLEDFKTGDSRSGLEALKYAAAGGESLARWKLAKMYANGDGVPQD
                     DEKAYDYFEQIVENYDEDDSSLRERAVVSSAFVAVGAYSLNGIPNTHVMSDPMRALEM
                     FQYAATNFGNADAQYNLARMYLDGVGVTRDAGQAARWLHLAAEKNHIEAQALLGQLLF
                     TGREGVARQRGLGLMWLTLAQQAAATDKKDQWIFDLYQKANAAADDTDKQIAALYIAD
                     YKKRQRD"
     gene            complement(7017..>8005)
                     /locus_tag="VGB93_12780"
     CDS             complement(7017..>8005)
                     /locus_tag="VGB93_12780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0146990.1"
                     /translation="VARNIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHECG
                     IDFDLFAVAEVFQRTPYIADLKPGGKYVAKDMFEVGGIPLLMKTLLDHGYMHGDCLTV
                     TGRTIAENLASVKWNPDQVVVYPADKPLSPTGGVVGLKGNLAPEGAIVKVAGMEKDSL
                     VFEGPALCFDNEEECFDAVENRRYEPGCVLVIRYEGPRGGPGMREMLSTTAALYGQGM
                     GNKVALITDGRFSGATRGFCIGHVGPEAAVGGPIALLRDGDIIRIDAIDHTIEVKLSD
                     EELAERKKSWQPRSTNYGSGAIWKYAQQVGSARYGAVTQPGGGGEVECYADS"
BASE COUNT         1697 a         2370 c         2336 g         1602 t
ORIGIN      
        1 agcgcttgcc ccgcgcatgg gacgcgcgga ggctgcatgt acaagcccgg cgctgcgcgc
       61 cgtccagaca gccgaggctt taggcctcgc cgccacaatc gatccgttgc tcgcggatat
      121 cgatcttgga gattgggccg gcaaatctct cgtcgaactg gaggccgccg atgcggctgg
      181 tctctcgcaa tggatagaag acccgtccgc cgcgccgcat ggcggcgaat ccgtcacaca
      241 tttgctgaca aggatcgcga gctggctggc caacatcggt gctggcgatg atcgaatcat
      301 cgccgtgacc cacgcggctg tgattcgcgc tgccgcgatc atcgcgctcg acgcaaatcc
      361 gaaatcgttt tggcggatcg acatcgcgcc gctgggtttc tcacgatttc atgcgcgtgg
      421 aggccgctgg acggtgcaca gcctgaacgc tgaggctgat tgatctgctc agcctggcgc
      481 gtcgcagcat cgagcatcgc ttgtgcggcg ggacacgcgc tgtcgggcct tcgccttgac
      541 cgccacgacg attgcttctc aaatccggcc gccggcgaga accggaaaaa ggggagaacg
      601 aaatgtctgc tttagggctc gtggttgaac tcgtcgccgg cgcgatcggc ggaaacggta
      661 ttgcctcagt cgccaagaac ctcaacttgg gaacgctcgg caattcgctt gtcggcgcca
      721 tcggcggcgc ggccggcacg tcattcctcg ggatcatcat cccggcgctg gagagcgtcg
      781 gtcatgcgga tatcgggaca ttggctggcc aagcggtcgg cggcggcgtg ctcggcgcgg
      841 tgctaacggc gatcgtcgca gccatccgca atgccagttc cggcgcgccc gcggcctgac
      901 cgggcatcaa ctgatttttg tttaaagaat caggccgaag gcaagtcgcc catacggcca
      961 agaggacgac gcaaagtccc cgtgaaaggt cccgtggcgt tctcgggatg tgccaacagc
     1021 ctgttgcaca gcccgagcga agccttatcc tgctgctgag cgacccggct tccccagcaa
     1081 ttgcggtcga gagtctctga tcgacgactc ttcgagccgt gataattttg cggcacctat
     1141 ggcccagcat caccccactc tgggtttttg gttgttcgcg ggtatcgccg cgacagcggg
     1201 cgtcgcctgc ctcttgttcg cgatagactc ttcaagaggc gacaaagaga cggtcgcaca
     1261 gagcaatcca gagacacgtt gcactgaaaa ttggaaaatg tgccgagatc acgccgatct
     1321 cgtcaactac agttcaaagg ccgacgatgc gaggcatgcc tgcattgctg ccgtgaatga
     1381 agccggaaaa tatggccacc cgaaatggtg cagtggctgg ttgtgcgagg atttcgccaa
     1441 ctatgaaaca ggtacgaatg cgccagatga cggcttgatc actctgatag acgatcatgt
     1501 acaaatgcaa aatggctccg gcgcacggat ccactctacc acatattgca catacaacat
     1561 tagaacagac aaagtcgtca ctctgaacat cggcgcaaat tagtcggttc gcaaactggt
     1621 atgcgaacga ggcttgagtg agcaacgaaa tcggccctgc agtgcagtat cggtctcggc
     1681 cgacgcgaaa ggcgacatag ccttcgatct atgccacttt aatctaaggg gggctgacgt
     1741 aagggagagt gacatgagta gatgcacgat tgccctgata ttaggtctcc tatgttgccc
     1801 cttgattttg cttcccacgc aggcacaaag cgccatctct gagcaggacg cgcaatcgat
     1861 tgctgtagat gcttacatct acctttaccc gctcgtaacg atggacataa cacgcaggca
     1921 gcttacgaat gtcgaactcg gtaaggggtt cggcgcgcct atgaacgtgt tcgccaacat
     1981 tccaacattt ccaacggcag atatgaaagc ggtcgtgtga cccaatttcg acacgctgtg
     2041 ctcgtccgtt ttcctcgatc ttacgaagga gccagttgtg gtctccgtcc ccgacacagg
     2101 cggacgctat taccttttac ctatgctcga catgtggtcg gacgtttttg catctctggg
     2161 ttggcgtacc acgggaacgc aggcgggcaa tttcctcgtc acgccacgcg gttggagcgg
     2221 aaccgttcct gcaggatttt cgcaaatcga agcgcctact cgctatgtct ggattattgg
     2281 ccgcacaaag actgacggtc cgcaggacta cgatgcggtt cacaaaattc aggccgggta
     2341 caagatcacg ccgctctctg catggggcaa gccgccgcag ccggtcgaag ttaagttcga
     2401 tccgagcgta gatatgaaga cgcctccaaa attgcaggtc gatagaatgc agggcgacga
     2461 atatttcgct tatgcagccg gagttcttaa agtgcagccg ccgcatctca cggatggacc
     2521 gattattgcg cgaatgaaac ggataggagt cgagccacgc aaaagcttcg acatcagcaa
     2581 agtcgatccg gtagtgagaa aggcgttgga agacgccccg gtgacggcga agaaactcat
     2641 ggaatggaaa cttgcgacgc ttgctcgtgt cgcgaacaat tggtccatga acacagacac
     2701 catgggcgtg tacggcaact actacctcaa acgcgccatc gtcgctaagc ttggtcttgg
     2761 tgcaaacctg ccggaagacg cgatatactc gcttaacatg ggcgatgaaa gcggcgctcc
     2821 gctcaacggc acgaacaatt acgcgatcca tttcgacaaa ggtgctacgc cgccgatcaa
     2881 tgccttttgg tcgatcaccc tctacgacag cgatggtttc caggtcgcca acagcctcaa
     2941 tcgtcttgcc gtgagcagtt ggatgccgtt caaatataac ccagatggct cgctcgacct
     3001 ctacttccaa aatgaaagtc ccggagcgga caaagaagat aattggcttc ccacgccgaa
     3061 gggcccctac aacttgacga tgggcgcgaa agccgatgct cattccagta ccccgagcat
     3121 cggctgatcc gatcagtgcg aaggcgacaa cagcgcgttt ggccgaactc gggtgcagtg
     3181 atgaagagat cgccgcgatt accggccatt tggacccgaa gcagatcaaa acctatacgc
     3241 gaggcgcgag acgcaaaatc accgcgggca gcgcgaatgc gcaagctcac tgggcaaagg
     3301 tcagtgaagg aagcgagcac caaaatgtct caccttgctt ttaggtgaga aaaatagaaa
     3361 aatatcaaca aaatcaatga gaaaaaagga atggtgcggc caagaggact cgaacctcca
     3421 cgggtctccc cactagcacc tcaagctagc gcgtctacca attccgccat ggccgcggaa
     3481 ggccggtgat ctaacaaacc ccccctgctc tgacaaggct ggtgcggggt tttcctgccc
     3541 ttcggcgctt gattttgtgc ccgcacggct caaattgcgc ggaatatgta ttttacgtct
     3601 gcgccgacgc gaattccggc gcgtttgagc caaaaagcat gagtgagtcg ttgcaacgtc
     3661 aggatatcgt ccgcgatcta cggaatcctg cgagcgcgcc ggtcgaatgg cgcgtgcttg
     3721 acggcctcac cggctatgac gatgcgctgg cgacgatgga ggcgcgtgtc gcggcgattg
     3781 cggcgggaaa agccgcggag caagtgctgc tgctcgaaca tccgccgctt tacaccgccg
     3841 gaacgtcggc cgcagcatcg acagacgatc tactccgttt cccgctgcat aaagccggcc
     3901 gtggcggtca gatcacctat catgggcctg gtcagcgcgt cgcctatgtg atgctcgatc
     3961 taaaccgccg ggggccggat gtccgtgcct tcgtcgctgc actcgaagac tggatcatcg
     4021 cgacgctggc gcatttcgac gtgcatggcg agcggcgcga ggatcgcgtc ggcgtctggg
     4081 ttgcgcgacc gtgcaagccg cgcggcccaa acaacgaagc cgccgaggac aagatcgccg
     4141 cgatcggcat ccgaattcgc cgctgggtta catttcacgg catctcgctg aatgtcgcgc
     4201 ccgacctctc gcattattcc ggcatcacgc cctgcggcat cgccgccccg catctcgacc
     4261 tcaccagcct gcgcgacctt ggcgtgacag cgacgatggc ggaggttgat gaggttctcc
     4321 gccagacctt tgagtcgact ttcggcgcgg gccgctgaat tcaggccgct ttcagcactg
     4381 aggtttcctg caacgccttg tcgagcgccg cctgccgcgc gtctggaccg agctgaacgc
     4441 cttcggcgac gatgaattgc agattggtga tgccaacgaa agagaagacc gagcgcagat
     4501 aggtctcgac atgttcgaac gcggctgccg gctggcccga gccatatagg ttgccgcgcg
     4561 aaatggcgat gatcactcgc ttccccgcgg cgaggccctt cacgccctcg ggaccatagc
     4621 tgaaggtctt gcccggtacg atgacgcggt cgatccaggc tttgagctgg ctcgggacgc
     4681 cgaaattata catcggcgcg ccgatcacaa cgatatcggc ggcgaggaat tcatcgagaa
     4741 tggcgtcgct tgccgcgcgg cctgcagcga actccggtgc ggcaccggcc atggccgaaa
     4801 tcgggtgcga cgagggaaaa ttggccggcg tgacgtgatc cggcgcagcg ccgatcagat
     4861 cgcgctcgat gatatgcaag cccggatgcg tctggcgcag gcgctccgtg acagcggcgg
     4921 tgatcacgcg gctcacggaa ttcggaccgg taatgcttga atcgatttgc agcaggttca
     4981 taatccacct cggtatatgt ttgtaacctg gactaattac ggtactgctg ctaaaccccg
     5041 caaggaggca catttttggg tgttgcagac atggacgtga ctatcgaggt ccccgcggtc
     5101 gagcattcgg actgccgcag ggtgagtcag cttcttgccc gcgtcggcga caagtggagc
     5161 gtgctcatca tcatgaccct tgccgccggg ccgaagcgct tcagcgagat caagcgcagc
     5221 gtcgacgcga tttctcagcg catgctgacg ctgaccttgc gcggcctcga acgcgatggc
     5281 ctggtgacgc gcaaggtctt cccaacaatc cctccgaaag tggaatacag gctgagcgaa
     5341 cttggctgct cgttgtgcga accggtgatg gcgctcggcc aatgggcgtt ggcgcatgtc
     5401 gtggagatcg ataccgcgcg cgaaaaattc gaccgcgaga gcggcgcggc ttaagagcta
     5461 tatcgtccgc agcagacgga agccgcttgg cggctggctc gtaagctcat ccggctttgg
     5521 ctcatacaca ctgagatcgg tcacgcgggc atatccggct cgcgccgaca tgcggcgacg
     5581 attttttcga tgccggtcat ctttcgcgac tcacgagtca aaatgagaca gaacacttaa
     5641 cgcgccacgc gcccgatcgc atcctttgtg caattttacg ggcgtgcttg tggctgccac
     5701 ctcgcgtcaa cacacaatct agcccggaac aaaccgccaa ttcttgacga tcagtgattc
     5761 gtcgctggta tgttcgcgct ttcaccgaga cgttaatgga aagaggtgcg agcgacctgc
     5821 ggcccgtgtc gtcagtgtcg aacgcaaggc ggatttttta gatgcgcgga taaaatctgc
     5881 tgcgctcttc atgattcaaa acgacacaaa acacttaacg cacctaatgt gcgattgcat
     5941 ccacagcgcg attttacgag cgagcttgta ggcatcgccc cgcgtcaaca cacaatctag
     6001 ccccgaacaa accgccaatt cttgacggtc agtgattcgt cgctggtatg ttcgcgcttt
     6061 cgccaagaca ttaacgcgat ggccatcggc cgaagtccga cacggacttt gaaacgcaaa
     6121 acgccatcca gcggtaactg gatggcgaac tcgcaattga agaattgttg tcgcgcgtct
     6181 aatcgcgctg gcgtttctta taatcggcga tgtaaagcgc ggcgatctgt ttatccgtgt
     6241 cgtccgccgc ggcattggcc ttttgataaa ggtcgaatat ccattgatct ttcttatcag
     6301 tcgctgccgc ctgctgcgcc aatgtcagcc acatcaggcc gaggccgcgc tggcgggcga
     6361 cgccctcgcg tcccgtgaac agcaattgcc cgagaagtgc ctgcgcttcg atatggtttt
     6421 tctcggccgc aagatgcagc cagcgcgccg cctgacctgc atcgcgcgtg acaccgacgc
     6481 catcgagata catgcgggca agattatatt gcgcgtcggc attgccgaaa ttggtcgccg
     6541 catattggaa catttcgagc gcgcgcatcg gatcggacat cacatgcgta ttcggaattc
     6601 cgttcaggct ataggcgcca acggcgacaa acgcgctcga caccaccgcg cgctcgcgca
     6661 gcgaggaatc gtcttcgtcg taattttcga cgatctgctc gaaataatca taagccttct
     6721 cgtcgtcctg tgggacgccg tcgccattgg cgtacatttt cgcgagcttc cagcgcgcga
     6781 gtgactcgcc cccggccgcc gcatatttca acgcctcgag tcccgagcga gaatcgccgg
     6841 ttttgaaatc ttcgagtcca gcgcgcaacg cagcctcagg atctttgaag atctgcagcg
     6901 gcatcttgtc gacgctcgtc gatgattgcg gcgtgccgtc gaatgcaacg gtgacggccg
     6961 gcgcggcgac aagcgtcgtg aagaacgcga cagcagagag ggctatgagg cttggcctag
     7021 ctatctgcat agcattcgac ctccccgccg ccgcccggct gagtcacggc gccataacgc
     7081 gccgaaccca cctgctgggc atatttccaa atcgcgcccg aaccgtaatt ggttgaccgc
     7141 ggctgccagg atttcttccg ctccgcgagt tcctcgtcgg acagtttgac ctcgatcgtg
     7201 tgatcgattg catcgatgcg gatgatgtcg ccgtcgcgca ggagcgcgat cggaccgccg
     7261 acggcggctt ccggcccaac atgaccgatg caaaatccgc gcgtcgcgcc ggagaaacgt
     7321 ccgtcggtga tcagcgcgac tttgtttccc atcccctgcc cgtagagcgc ggcggtcgtc
     7381 gacagcatct cacgcatgcc ggggccgccg cgtggacctt cgtagcggat cacgagcacg
     7441 cagcccggtt cgtagcggcg attctcgacc gcgtcgaaac attcctcttc gttgtcgaag
     7501 cagagagctg gaccttcgaa aacgaggcta tccttctcca tgcccgcgac cttcacgatc
     7561 gcgccttccg gggccagatt acccttgagg ccaacgacgc caccggtcgg gctcaatggc
     7621 ttatcggccg gatagacgac gacctgatcc ggattccatt tcacgctggc gaggttttcc
     7681 gcgatcgtgc gcccggtgac cgtcaggcag tcaccgtgca tgtagccgtg atcgagcaag
     7741 gtcttcatca aaagcggaat gccgccgacc tcgaacatgt ccttggcgac atatttaccg
     7801 cccggcttca gatcagcgat gtagggcgtg cgctggaaaa cttcggcgac ggcgaaaaga
     7861 tcgaaatcga tgccgcactc atgcgcgatc gccggcaagt gaagcgccgc attggtcgaa
     7921 ccgcctgagg cggcgacgac ggcagcggca ttttccaacg ccttgcgggt gacgatgtcg
     7981 cgcggacgga tattgcgggc gacca
//
LOCUS       DASWOG010000621         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_107509,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000621 DASWOG010000000
VERSION     DASWOG010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107509"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGB93_12785"
     CDS             <1..580
                     /locus_tag="VGB93_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0146991.1"
                     /translation="PDSKSAPDTSHPAAIVASATAAPAKTDLKKQPALSATQALAMQL
                     QQEIAKLIGSTGKDAAKPATEVKAVDEGILISLTDQANFAMFAIGSAEPQPKTVHIMD
                     KVARALKSLSGDLIVGGFTDGRPYRTGVYDNWRLSTARAQMAYYMLVRGGVDQKRIVR
                     IEGYADRRLKVPSKPLADENRRIEIFLKEAKS"
     gene            577..>1665
                     /locus_tag="VGB93_12790"
     CDS             577..>1665
                     /locus_tag="VGB93_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotC"
                     /protein_id="HEY0146992.1"
                     /translation="MSRRALTAIYLAFAFAAGPLCAAPSKPPYELVRSLHALQDQIVL
                     GNRTARIAVSSLATELATRLLSYNPSVWREPRNARAVVTYVLSGGSPSVAEKILASGD
                     CAPQEKTLVEAAVAYSEGHITRARSLLANVDARTLEPIVGGHIALAQAALVASKDPYK
                     AIELLDVARVLAPGTLIEEAALRREIFIINATGDFDRFISLSGDYIRRFRNSTYADNF
                     REHFSDAIARLNIVGDSDQLAQIDDALSSLPPDDQLKLYLMIARASILMGKISAAEFA
                     AAKAESLASPDSTESGRAQLYDGAAQIFTPRYARGDDMLDTLNAAHLSREEVGLKDAA
                     QSVSQQIHEWPQVDARQPLDSAGVQPLPR"
BASE COUNT          345 a          489 c          502 g          329 t
ORIGIN      
        1 gcccgacagc aaatcggcgc cggatacgtc ccatcccgcg gcgattgtcg cgtctgcgac
       61 ggcggcaccg gcgaaaacag accttaagaa gcagccggcg ctcagcgcca cacaggctct
      121 cgccatgcag ctgcaacagg aaatcgccaa gctcatcggt tctaccggga aagatgctgc
      181 aaaaccggcg accgaagtaa aggccgtgga tgaaggcatc ctgataagcc tgaccgatca
      241 agcaaatttc gcgatgtttg ccatcggctc ggccgagccg cagccgaaga cggtgcatat
      301 catggataag gtcgcgcggg cgctcaagag cctctcaggc gacctcattg tcggcggatt
      361 tacggatggg cggccatatc gaaccggagt ctatgacaac tggcggcttt cgacggcgcg
      421 cgcgcagatg gcgtattaca tgctcgtccg cggcggtgtt gaccaaaagc gcatcgtgcg
      481 aatcgaaggt tacgcggatc gtcggctgaa agtgccgagc aaaccgctgg cagacgaaaa
      541 ccgtcgcatc gagatattcc tgaaagaggc taagtcatga gccggcgcgc cttgaccgcg
      601 atatatctgg cttttgcctt cgccgccggc cccctttgcg cggcgccgtc gaagccgcct
      661 tacgaactcg tgcggtcttt gcatgcgttg caagatcaga tcgtattggg caatcgcacg
      721 gcgcgcatcg ccgtttcgag tcttgcgacc gaacttgcga ctcggctgct ttcctacaat
      781 ccgtcggtgt ggcgtgagcc gcgtaacgcg cgcgcggttg tgacttatgt cctcagcggt
      841 ggctctccaa gcgtcgccga gaagatactc gcgagtggag attgcgcgcc gcaggaaaag
      901 acgcttgtcg aggcggcggt ggcttattcc gaaggccata tcacacgcgc gagatcgcta
      961 ttggcaaatg tcgatgcgcg cacgctcgag ccgattgtgg gcggtcatat cgccctggcg
     1021 caggcagcgc ttgtcgccag caaggatcca tacaaggcga tcgaacttct cgatgtcgcg
     1081 cgggtcttgg cgccgggtac tcttatagaa gaagccgcct tgcggcgtga aatcttcatt
     1141 atcaatgcga ccggagattt cgatcggttc atcagcctgt ccggcgacta tatccggcgc
     1201 tttcgcaact cgacatatgc tgacaatttc cgcgagcatt tttccgatgc aattgctcgg
     1261 ctcaacatcg tgggcgattc ggaccagctt gcacagatcg atgatgcttt gagcagtttg
     1321 ccgcccgatg accagttgaa gctttatctc atgatcgccc gtgcctcgat tctgatggga
     1381 aaaatctcgg ccgcggagtt tgccgcggcg aaagcagaga gcctggcctc gcccgattca
     1441 accgagagcg gccgcgcaca actttatgat ggcgcagcgc aaatatttac accgcgctac
     1501 gcgcgcggcg acgacatgct cgatacgctg aatgccgcgc acctttcgcg tgaagaggtc
     1561 ggccttaagg acgccgcgca gtcggtctcg cagcaaattc atgaatggcc gcaagtcgac
     1621 gcaaggcagc ctttggacag tgcaggggtg cagcccctgc cgcgg
//
LOCUS       DASWOG010000622         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_15623,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000622 DASWOG010000000
VERSION     DASWOG010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15623"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGB93_12795"
     CDS             complement(<1..736)
                     /locus_tag="VGB93_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY0146993.1"
                     /translation="MNGVNEIRTAFLDYFGRHGHEIVASSPLVPRNDPTLIFTNAGMV
                     QFKNVFTGIEKRPYTRAATAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KAEAIELAYKLMTQVFGLPKDKLLVTVYHTDDEAFDLWKKIAGFPDSKIIRIPTSDNF
                     WSMGDTGPCGPCSEIFYDQGEKLAGGPPGSADEDGDRFLEFWNLVFMQYEQLAPGERV
                     GLPRPSIDTGMGLERIAALLQGVQSNY"
     gene            complement(873..>1904)
                     /gene="recA"
                     /locus_tag="VGB93_12800"
     CDS             complement(873..>1904)
                     /gene="recA"
                     /locus_tag="VGB93_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0146994.1"
                     /translation="DKTKALDAAVSQIERAFGKGSIMRLGKNPVVEIETISTGSLGLD
                     IALGVGGLPRGRVVEIYGPESSGKTTLTLHVIAEAQKAGGVCAFIDAEHALDPIYARK
                     LGVNLDDLLISQPDTGEQALEITDTLVRSGSVDVLVIDSVAALTPRAEIDGEMGDSQP
                     GLQARLMSQALRKLTASISKSKTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVR
                     LDIRRIGSIKERDEVVGNQTRVKVVKNKVAPPFKQVEFDIMYGAGISKVGELIDLGVK
                     AGVVEKSGAWFSYDSQRLGQGRENGKTFLKNNPEIAAEIELAIRENSGLIAERILDPG
                     ETEETELEG"
BASE COUNT          383 a          562 c          567 g          392 t
ORIGIN      
        1 cgtagttcga ttggacaccc tgcaggagcg cagcgatacg ctcgagcccc atgccggtgt
       61 cgatcgacgg acgcggcaag cccacgcgct cgccgggggc gagctgttcg tattgcatga
      121 agaccagatt ccagaattcc agaaaacggt cgccatcctc gtcagcactg ccaggcggac
      181 caccggcgag cttctcgccc tgatcgtaga aaatctccga acaggggccg caggggccgg
      241 tgtcgcccat cgaccagaaa ttatcggagg tcgggatgcg gatgatcttc gaatcgggaa
      301 aaccggcgat ctttttccag agatcgaagg cttcgtcgtc ggtgtgataa acggtgacga
      361 gcagcttgtc cttgggcaga ccgaagacct gcgtcatcag cttgtaggca agctcgattg
      421 cttcggcctt gaaataatcg ccgaaggaaa aattaccgag catctcaaag aacgtgtgat
      481 ggcgtgcggt atagccgaca ttgtcgagat cgttgtgctt accgccggcg cggacgcact
      541 tctgcgccgt cgcggcgcgt gtgtaggggc gcttctcgat gccggtaaag acattcttga
      601 attgcaccat gcccgcattg gtgaatatca acgtagggtc gttgcgtggg acgagcggcg
      661 aggacgcgac gatctcatgc ccgtggcggc cgaaatagtc gagaaacgca gtcctgatct
      721 cgttgacgcc gttcataatc tgcatccatg cacgcggcga aggatgaaat tcgccgcggc
      781 catccaggcg gaaaggggag cacgtcttct agctttccgc ttccgccctg tcgagcgccg
      841 gcgcggcgtc gatgcaggag gctggaggca gatcagcctt cgagttcggt ctcttcggtt
      901 tcgccgggat cgagaatgcg ctcggcgatc aggccggaat tttcgcgaat cgccaattcg
      961 atttcagcgg cgatctcggg attatttttc aaaaatgtct tgccgttttc acggccctgg
     1021 ccaagtctct ggctgtcata agagaaccag gcgccggatt tctcgacaac gccggccttg
     1081 acgccgaggt cgatcaattc gccgaccttg gagatgcctg ccccatacat gatgtcgaat
     1141 tcgacctgtt tgaagggcgg cgcgaccttg ttcttcacca ctttcacgcg ggtctgattg
     1201 ccgacaacct catcgcgttc cttgatcgaa ccgatgcggc gaatatcgag ccggaccgag
     1261 gcatagaatt tcaacgcatt gccgcccgtc gtcgtctccg ggctgccgta catgacgccg
     1321 atcttcatgc gaatctgatt gatgaaaatc accatcgttt ttgacttcga gatcgacgca
     1381 gtgagcttgc gtagcgcctg gctcatcagc cgcgcctgca ggcccggctg gctgtcgccc
     1441 atctcgccgt cgatttcggc gcgcggcgtg agtgcggcaa ccgaatcgat caccagcacg
     1501 tcaaccgagc cggagcggac caaagtatcg gtaatctcaa gcgcctgctc gcccgtatcg
     1561 ggctgcgaga tcaacagatc atcgagattg acgccgagtt tgcgggcata gatcggatcg
     1621 agcgcatgtt cagcatcgat gaaggcgcag acgccgccag ccttctgggc ctcggcgata
     1681 acatgcagtg tcagcgtcgt cttgcccgag gattccggcc cgtaaatctc gacgacccga
     1741 ccgcgcggca gaccgccgac gccaagcgcg atatcgagcc cgagcgaccc cgtcgaaatc
     1801 gtctcgatct cgaccaccgg gttcttgccg aggcgcataa tcgagccttt gccgaacgcg
     1861 cgctcaattt gagagacggc ggcgtcgagc gccttggtct tgtc
//
LOCUS       DASWOG010000623         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_33849,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000623 DASWOG010000000
VERSION     DASWOG010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33849"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..789
                     /locus_tag="VGB93_12805"
     CDS             436..789
                     /locus_tag="VGB93_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0146995.1"
                     /translation="MHKSAQILDLSHLAAQTGGDHALQREILTLFVRQSQEILTLLQD
                     DDSLPQVRADLAHKLKGSARTVGAFEFAEAAEAVETGLRAGHAMPVALRDLSADADAA
                     DAEISRYLTSLSHNG"
     gene            940..1260
                     /locus_tag="VGB93_12810"
     CDS             940..1260
                     /locus_tag="VGB93_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0146996.1"
                     /translation="MVHITFIDSSGEKRPVEAEEGSTVMEAAIRNSIPELTAECGGAC
                     ACATCHVYVDEAFVSLTGEPSPQEEDMLDFAYDVQPNSRLSCQIKITPEIDGLVVRTP
                     ARQG"
     gene            1385..>1828
                     /locus_tag="VGB93_12815"
     CDS             1385..>1828
                     /locus_tag="VGB93_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0146997.1"
                     /translation="MVDNIDTDVVFVGAGPSALFTVFELGLLDIRCHLIDILPRAGGQ
                     CSELYPEKPIYDIPAHPMVTGTGLIDNLMAQIEPFRPTFHFDQLVTELESLGTPEAPL
                     FRLKTDAGTTFTAKAVIVAAGGGSFQPKKPPIEGIEAFEDKSVFYA"
BASE COUNT          398 a          563 c          500 g          367 t
ORIGIN      
        1 cagccggcgc gctgtcggag aggtttaggg cctcttcaat tgccgacagc gctgcagccg
       61 ccggatcttg cgccttattg gacgtcgcca ttttgacctc ttacgcaaac gaaatacgca
      121 ccgaacaaga acgctacggc agggattccc cgtccgccaa cggcgaaaac acaaatattc
      181 cattaaccaa gggcagttta catccggacc tgggcgatgg aaaccgccac tgccaaaggg
      241 tttggaaacg tcgttaacgc gttattcacc ataggcggaa agatttaccg ccaagtcagc
      301 gttctagtca tctttgcgtg cgctatccga aatttgtcga gcaaaacccg tgcgttggca
      361 agccacactt ggggtcggcg ggataattaa ctggggatgg atgcgagagc gcccaaagct
      421 gcacgaggga ctatcttgca taaatcggcg caaatactcg atctgagcca cctcgcggcg
      481 caaacgggcg gtgatcacgc ccttcaacgc gagattttga cgcttttcgt ccgccaatcg
      541 caggaaattc tgaccctgct tcaggacgac gactccttgc cgcaagtccg tgccgacctc
      601 gcccataaac tgaaaggttc agcccggact gtcggcgcct tcgaattcgc cgaggcggca
      661 gaagccgtag aaaccggctt gcgggctggc cacgcgatgc ccgtcgcact tagagaccta
      721 tccgccgatg cggacgccgc cgacgccgag atcagccgct atttaacgag cctttcccac
      781 aacggctgag cgcgctctgc tgcgacgcaa cataaaagct aactcaaaat cgcgattttg
      841 ctggcaaatc gccgccccgg cgaagacaag gctgttcatc gcgcaagctc ttcattaaga
      901 aagcgcgcaa tcctgtccga accccctgag aaagcgtcaa tggtccatat taccttcatc
      961 gattcatctg gcgaaaaaag accggttgag gccgaagaag gctcgacggt gatggaagcg
     1021 gcgatccgca attcgatccc ggagctgacc gcagaatgcg gcggagcctg tgcctgcgcc
     1081 acctgccatg tctatgtcga tgaagctttt gtttccttga cgggcgagcc cagcccgcag
     1141 gaagaggata tgcttgattt tgcctatgac gtgcagccca attcacggct ctcgtgtcag
     1201 atcaaaatca cgcccgagat tgacggcctg gtcgtcagga cgccggcgcg ccagggctaa
     1261 ttccccgagg ggatttcccg gcgcttcttc cgcggatcgc cagggacgcg cctgcgagcc
     1321 ggcaaaatct gccaagccgc cgggattgca gcctaggcgc atatgaattt tcaaggagcc
     1381 cttgatggtt gataatattg ataccgacgt cgtcttcgtg ggcgccggtc cctctgctct
     1441 cttcacggtg ttcgaactcg gcctgctcga tatccgctgc catttgatcg atatcctgcc
     1501 ccgcgccggc ggccagtgct ccgagcttta tcccgagaag ccgatctacg acattcccgc
     1561 ccatccgatg gtgaccggca ctggtctcat cgacaacttg atggcgcaga tcgaaccttt
     1621 ccggccgacc ttccatttcg atcaattggt caccgaactc gaaagcctcg gcacgccgga
     1681 ggcgccgctg ttccgcctca agaccgacgc cggcacgact ttcacggcaa aggcggtgat
     1741 cgtcgccgcg ggcggtggtt cattccagcc gaagaagccg ccgatcgaag gcatcgaggc
     1801 ctttgaagac aaatccgtct tctacgcc
//
LOCUS       DASWOG010000624         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_327586,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000624 DASWOG010000000
VERSION     DASWOG010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327586"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VGB93_12820"
     CDS             <1..1248
                     /locus_tag="VGB93_12820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0146998.1"
                     /translation="FADFGHKVCCIDNDPAKIEALKSGKMPIYEPGLAELVMTNVRQN
                     RLTFADDLSAVREAQAVFIAVGTPSRRGDGHADLTYVYQAARDIAAAIDGFTVIVTKS
                     TVPVGTGDEVERIIREVRPDAEFAVVSNPEFLREGAAIADFKRPDRIVIGVEDPRAEA
                     VMREVYRPLYLNQAPILVTSRRTSELTKYAANAFLATKITFINEIASLCEEVGANVQE
                     VARGIGLDNRIGSKFLHAGPGYGGSCFPKDTQALIKTAQDYGAPIRIVETVAAVNDQR
                     KRAMARKVLAACGGNVRNKTIAVLGLTFKPNTDDMRDSPAIAIITALQDAGAKVNAFD
                     PEGMDQARHYLDKLNYCSDPYSCCNDASALVIVTEWDAFRALDLDRVKSLLIEPILVD
                     LRNIYSPDELRRKGFKYLSIGRP"
     gene            complement(1256..>2363)
                     /locus_tag="VGB93_12825"
     CDS             complement(1256..>2363)
                     /locus_tag="VGB93_12825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0146999.1"
                     /translation="KFMLKRRQKITAEQQTSISTQDYLRTHFCASPFEQLETTPTGLA
                     YVCCPVWLPTPIGALDTDPQKLWNGDIAKKLRKSIIDGSYRYCNHVHCAAITSRTLMP
                     RDSARAQTIIARHESGTTSLPEHVKLSHDKSCNLSCPSCRSSTYLANSAKQAKLDDLT
                     EKTLVPLLREAKSVLVTGSGDPFGSKHFRNLLKRLNGGEFPDLKIDLISNGQLLDPRA
                     WTELGLKNRVRNVEISIDAAQAETYAIVRRGGTFARLLKNLAFLRDMRRSGEIAQLTY
                     SMVVQALNYKEMPAFVRMSEDYAADIISFNMIRQRDVFAKDEYVEAFIGSPDHPEYED
                     FLKVLNAPELVQHNVLIGNVREYARPSVLAEAAE"
BASE COUNT          464 a          687 c          716 g          496 t
ORIGIN      
        1 ttcgcggatt tcggtcacaa ggtctgctgc atcgacaatg atcccgcgaa gatcgaggcg
       61 ctgaagtccg gcaagatgcc gatctatgag cccggcctgg ccgaactggt gatgaccaac
      121 gtccgtcaga acaggctgac attcgccgat gatctctcgg ctgtgcgtga ggcgcaggcg
      181 gtgtttatcg ccgtcggcac gccctcgcga cgcggcgacg gccatgccga tctcacctac
      241 gtctatcagg cggcgcgcga cattgccgcg gcgatcgatg gcttcacggt catcgtgacg
      301 aaatccaccg tccccgtggg caccggcgac gaggtcgagc gcatcattcg cgaagtgcgg
      361 ccggacgccg aatttgccgt tgtctccaat cccgagttcc tgcgcgaagg cgctgcgatc
      421 gccgatttca agcggccgga tcgcatcgtc atcggcgtcg aggacccgcg cgccgaagcg
      481 gtgatgcgcg aagtctatcg gccgctctac ctcaaccagg ctccgatcct cgtcaccagc
      541 cgccgcacgt cggaactcac caaatatgcg gcgaatgcct tcctggcgac caaaatcact
      601 ttcatcaacg agattgcgag cctctgcgag gaagtggggg cgaatgtgca ggaggtcgcg
      661 cgcggcatcg gcctcgacaa tcgcatcggc tcaaaatttc tgcacgcagg acccggctat
      721 ggtggctcct gctttcctaa ggatacccag gcactgatca agacggcgca ggactatggc
      781 gcgccgatcc gcattgtcga gacggtcgcg gcggtcaatg accagcgcaa gcgggcgatg
      841 gcccgcaaag tgctcgcagc gtgcggtggc aatgtgcgca ataagacgat tgcggtgctc
      901 ggccttacct tcaagcccaa caccgacgac atgcgcgatt cgccggcgat cgcgatcatc
      961 acggcgcttc aggatgctgg cgccaaggtc aatgctttcg atcccgaggg tatggatcaa
     1021 gcgcgtcact atctcgacaa gctgaattat tgcagcgacc cttatagctg ctgcaatgat
     1081 gcctcggcgc tcgtcatcgt cacggaatgg gatgccttcc gggcgctcga tctcgaccgc
     1141 gtcaagtcgc tgttgatcga gccaattctc gtcgatctac gcaatatcta ttcgccggat
     1201 gagttgcgcc gcaagggctt caaatatctc tcgatcgggc ggccctgatg gccgcttatt
     1261 cggctgcttc cgcaaggacg ctcgggcgcg catattcgcg tacattgccg atcagcacgt
     1321 tgtgctggac gagttccggc gcattgagca ctttcaggaa atcctcatat tccgggtgat
     1381 ccgggctccc gatgaaagct tcgacatatt cgtccttggc gaagacgtcg cgctgacgga
     1441 tcatattgaa cgagatgatg tcggctgcgt aatcctccga catgcgcacg aaggctggca
     1501 tttccttgta gttcagcgcc tgcacgacca ttgaatatgt caattgcgca atctcgcctg
     1561 aacggcgcat gtcgcgcagg aaggcgagat ttttgaggag acgcgcgaac gtgccgccgc
     1621 gccgcacgat cgcgtaagtc tcggcctgcg ccgcatcgat cgaaatctcg acattgcgca
     1681 cgcggttctt gagtccgagt tctgtccagg cacgcggatc aagaagctgg ccgttgctga
     1741 tgagatcgat tttcaggtcg ggaaattcac cgccgttgag acgcttgagc agattgcgga
     1801 aatgcttgct gccgaatgga tcgccggacc cagtgacgag cacggacttc gcttcgcgca
     1861 atagcggtac aagagtcttt tcggtcagat cgtcaagctt tgcctgtttg gcggaattgg
     1921 cgaggtaagt gcttgagcgg cacgacggac aggagaggtt gcaggatttg tcgtgcgaga
     1981 gtttcacatg ctccggcagg cttgtcgtgc cggattcatg ccgcgcaatg attgtctgcg
     2041 cccgggcgga gtcgcgcggc atcagcgtgc ggctggtgat tgcggcacag tgcacatgat
     2101 tgcaataacg ataagagcca tcgatgattg acttgcggag cttcttcgca atgtcgccat
     2161 tccagagttt ctgtggatcg gtatcaagcg cgccgatcgg cgtcggcagc cagaccggac
     2221 agcagacata ggcgagcccc gtcggcgtcg tttcgagctg ctcaaacggg ctggcacaaa
     2281 aatgcgtgcg taaatagtcc tgcgtgctga tgctcgtctg ctgctcggcg gtgatcttct
     2341 gccgccgctt cagcatgaat tta
//
LOCUS       DASWOG010000625         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_309693,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000625 DASWOG010000000
VERSION     DASWOG010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309693"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="nuoK"
                     /locus_tag="VGB93_12830"
     CDS             <1..229
                     /gene="nuoK"
                     /locus_tag="VGB93_12830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0147000.1"
                     /translation="KNIIVILMSVELILLAVNINLVAFSAFLGDLTGQVFALFVLTVA
                     AAEAAIGLAILVTYYRIRGTIAVEDINLMKG"
     gene            235..2262
                     /gene="nuoL"
                     /locus_tag="VGB93_12835"
     CDS             235..2262
                     /gene="nuoL"
                     /locus_tag="VGB93_12835"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0147001.1"
                     /translation="MYQAMVFLPLAGFLIAGIFGRLIGPRSSELITTSLLFVAAALAW
                     VTFVGFLTNQNPSSIELLTSWFDSGALHVDWALRVDTLTAVMLVVVTTVSALVHLYSI
                     GYMHDDPSRPRFFAYLSLFTFAMLMLVTSDNLLQMFFGWEGVGLASYLLIGFWYQKPS
                     ANAAAIKAFVVNRVGDFGFTLGIFLVFILTNSVTFDPIFAAAPGLAHKTIHVFGADLD
                     AMTITCLLLFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSP
                     LFEQSPAALSFVTFIGGTTAFFAATVGLVQNDIKRVIAYSTCSQLGYMFVGLGVGGYS
                     LGIFHLFTHAFFKALLFLGAGSVIMAMHHEQDMRKMGGLASKIPVTFIMMTIGTLALT
                     GFPLTAGYFSKDAIIEASYVGHGWTATYAFILVTVAAGLTSFYSWRLVFMTFFGHSHW
                     QDAHEDHAHDHADIADAHGDHVAAAAHADHAHDDHAHDPHESPPVVLVPLFVLAFGAL
                     VAGFVFKHFFIGEGGPEFWKSALYYGPANHILEAMEHVPALVSLGPTLMMVGGFFVAL
                     YVYILAPGTAQKWADAMPGLYRFLLNKWYFDELYDRIFVRPAFWLGNLLWKGGDENII
                     DRFGPNGVAARVVDVTNRVVRLQTGYLYHYAFAMLIGVAALITYYLVGSLR"
     gene            2262..>3062
                     /locus_tag="VGB93_12840"
     CDS             2262..>3062
                     /locus_tag="VGB93_12840"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0147002.1"
                     /translation="MFGFGILSGLVVLPLVGAAFILTLRGDDKATQNNARWAALTTAI
                     ITFLFSLVVCSKIDFSSSAFQLVEEHRWFGDGLVYKLGLDGLSAPFILLTTFLKPFCI
                     LASWRSVQFRVKECMIAFLILETLLLGAFSALDLVLFYLFFEGGLIPMFLIIGIWGGK
                     RRVYASFKFFLYTLLGSVLMLIAIMAMYNFAGTTDITILLKTHFPARMQTWLWLAFFA
                     SFAVKLPMWPVHTWLPDAHVEAPTGGSVDLAAILLKLGGYGFMRFSLPM"
BASE COUNT          465 a          915 c          863 g          819 t
ORIGIN      
        1 caagaacatc atcgtcatcc tgatgtcggt cgagttgatc ctgctggcgg tcaatatcaa
       61 cctcgtcgcc ttttccgctt tcctcggtga cctcacgggc caggtcttcg cactgttcgt
      121 gctgacggtc gcggcggcgg aggccgcgat cggccttgcc attcttgtca cctattatcg
      181 tattcgcggt acgatcgcgg tcgaagacat caacctgatg aagggctgag ccggatgtat
      241 caggcgatgg tcttccttcc gcttgccggc tttctcatcg ccgggatttt cggccggctg
      301 atcgggccgc ggtcctcgga gcttatcacg acaagcttgc tcttcgtcgc ggcggcgctc
      361 gcctgggtga ctttcgtcgg ctttctgacc aaccaaaacc catcttcgat cgagcttctc
      421 acgagttggt tcgactcggg cgcgttgcac gtcgattggg ctttacgcgt cgatacgctg
      481 acggcggtca tgctcgtcgt cgtcacgaca gtgtcggcgc tcgtgcatct ttattcgatc
      541 ggctatatgc acgatgaccc gtcgcggccg cgtttcttcg cctatctgtc gctgttcacc
      601 tttgcgatgc tcatgctggt gacatcggac aatctgctgc agatgttttt cggctgggag
      661 ggcgtcggcc tcgcctccta tctgctcatc ggtttttggt atcagaagcc gtccgccaat
      721 gcggcggcga tcaaggcttt cgttgtcaac cgcgtcggtg acttcggctt cacgctcggc
      781 attttccttg tcttcattct gacgaattcg gtgacgttcg atccgatctt cgccgccgcg
      841 ccaggccttg cgcataagac cattcatgtc ttcggcgccg atctcgacgc tatgacgatc
      901 acctgtctgc tgctcttcat gggcgcgatg ggcaaatcgg cgcaattcct gctccatacc
      961 tggttgcctg acgccatgga gggcccgaca cccgtttcgg cgttgatcca cgcggcgacc
     1021 atggttacgg ccggcgtctt catggtcgcc cggctgtcgc ctttgttcga gcaatcgcca
     1081 gcggcgctga gcttcgtcac ctttatcggc ggcacgacgg cctttttcgc cgcgacggtc
     1141 ggcctggtgc agaacgacat caaacgcgtc atcgcgtatt cgacgtgttc gcagctcggc
     1201 tacatgtttg tcggcctcgg cgttggcggc tattcgctcg gcatttttca tttgtttacg
     1261 cacgccttct tcaaggcgct gctcttcctc ggcgcgggct ccgttatcat ggcgatgcac
     1321 cacgagcagg acatgcggaa aatgggcggc ctcgcgagca agatcccggt gaccttcatc
     1381 atgatgacga tcggcacctt ggcgctgacc ggttttccgc tgacggcagg ctatttctcc
     1441 aaagatgcga tcatcgaggc ttcttatgtg ggtcacggat ggacggcgac ttatgccttt
     1501 atcctcgtga cggttgcggc gggtctgacc tcattttatt catggcggct ggtgttcatg
     1561 acctttttcg gccattcgca ctggcaggac gcgcatgaag atcatgcgca cgaccatgcg
     1621 gacatcgctg acgcgcacgg tgatcatgtg gcggctgcgg ctcatgccga tcatgctcac
     1681 gatgatcacg cgcacgatcc gcatgaatcg ccgccagtcg tgctggtccc gcttttcgtc
     1741 ctcgccttcg gcgcgctggt ggcgggtttc gtgttcaagc atttcttcat cggcgagggc
     1801 ggccccgaat tctggaagtc tgcgctctat tatggtccgg caaaccatat ccttgaggcg
     1861 atggaacatg tgcccgcact cgtaagtctt ggcccgaccc tcatgatggt cggcggcttt
     1921 ttcgttgcgc tctatgttta tattctcgcg cccggtacgg cccagaaatg ggccgacgcc
     1981 atgcccgggc tttatcgttt tctgctcaac aaatggtact tcgacgagct ttatgaccgc
     2041 atcttcgtgc ggccggcttt ctggctcggc aatctgttgt ggaagggcgg cgacgagaat
     2101 atcatcgatc gtttcggtcc caatggcgtt gcggcacgcg tcgtggacgt caccaatcgc
     2161 gtggtgcggt tgcagaccgg ctatctctat cattacgcct tcgccatgct gatcggcgtc
     2221 gcggcgctca tcacctatta tctcgtgggg agcctgcgct gatgttcggt tttggcatcc
     2281 tctcggggct cgtagttctg ccgctcgtcg gcgctgcctt catcctgacc ctgcgcggcg
     2341 atgacaaggc gacgcaaaac aacgcgcgct gggcggcgct gacgacggca atcatcacct
     2401 tcctgttctc gctcgttgtt tgttcgaaga ttgatttcag ctcctcggcc tttcaactcg
     2461 tcgaggaaca tcggtggttc ggcgacggcc ttgtctataa gctcggcctc gacggtttgt
     2521 cggcaccttt catactgctg acgaccttcc tcaaaccctt ctgcatcctc gcatcctggc
     2581 ggtcggtcca gttccgcgtg aaggaatgta tgatcgcgtt tctgatcctt gagacactgc
     2641 ttctcggtgc gttctccgcg ctcgatctcg tgctcttcta tcttttcttc gagggcggtt
     2701 tgattccgat gtttctcatc atcggtatct ggggcggcaa acggcgcgtt tatgcgagct
     2761 tcaaattctt cctttacaca ctgcttggct cggttttgat gctcatcgcc atcatggcca
     2821 tgtataattt tgccggcacg acagacatca ccatcctgtt gaagactcat ttcccggccc
     2881 ggatgcagac ttggctgtgg ctcgccttct ttgcctcctt cgcggtgaaa ctgccgatgt
     2941 ggccggtcca cacttggctg ccagatgcgc atgtcgaggc gccgaccggt gggtcggtgg
     3001 atcttgcggc catattgctg aaattgggcg gatacggctt tatgcgtttt tcgctaccga
     3061 tg
//
LOCUS       DASWOG010000626         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_180339,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000626 DASWOG010000000
VERSION     DASWOG010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180339"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..469
                     /gene="ptsN"
                     /locus_tag="VGB93_12845"
     CDS             5..469
                     /gene="ptsN"
                     /locus_tag="VGB93_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY0147003.1"
                     /translation="MRLTDLIAHDAIIPSLRAGTKKLVLQELSEKAARISGLPPREIF
                     NALLQRERLGSTGTGNGVALPHAKLKKCQRIFGIFARLEKPIDFEAIDGEPVDLVLML
                     VAPEFAGADHLKALARVARVLRDVRIAAKLRAARDPAALYAVLTQEPTSNAA"
     gene            538..2187
                     /locus_tag="VGB93_12850"
     CDS             538..2187
                     /locus_tag="VGB93_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0147004.1"
                     /translation="MLSDAGKPIAVSSVPALLEQAASAFPDHNALDFFGRKLTYTDLA
                     HQVDRAARGFQRLGVVHGTRVGLCLPNTPYFVIAYFAVLKAGGIVVNFDPLQVAREIA
                     RQIEDSGTTIMVTLDVTKIYPKIAEALETTSLEKVVICSLCAALPTGKGLLFRLFKAR
                     ELARIPRNAKHVRFEKLFADRTPMTPVTINPVIDLAVLQYTGGTTGVPKGAMLSHANL
                     TANVAQIIDRVPVLRPGQESAIVLLPLFHVFGMTTSMNLCVALGFEMILLPSLDLPQL
                     LKLIVSKKPTLFPGVPSLFATLSNMAEHGTFDLSSIRFCISGGAPLPLETRQRFEALT
                     GCELIEGYGLTEASPVVAANPLDAPYKNESVGPPLKETIVEIRSLDDPMKILRAGERG
                     EICLRGPQIMAGYWRNLQETKAAFVEGALRTGDVGYLDQDGDLFLVDRIKDIIICNGF
                     KIYPRAIEEALVQHPAVDEAVAIGIADAVRGHVPKAFVTLLPGAATTPDMLLEFLKTH
                     LSRLEMPRVIEIRAALPKTNIGKISRKDLVLEEERLHAVSE"
     gene            2187..>3063
                     /locus_tag="VGB93_12855"
     CDS             2187..>3063
                     /locus_tag="VGB93_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0147005.1"
                     /translation="MATRRLKSQTKTSSEESLLPPRFAEFFTRRGWTPRQHQLALLKH
                     AEAGEDELLIAPTGAGKTLAGFLPTLIDLDRPRNRRGLHTLYLSPLKALATDVARNLE
                     IPIAEMGLNIRAETRTGDTAMSKRQRKRRDPPDILLTTPEQLALLLASADARFLFADL
                     KRVIIDELHSLTASKRGDLLSLDLARLRDIAPQMRATGLSATVRDPDELRRWLVSQSH
                     DALDHDGFGSDRSKTMNVIDSKNLEPDSREKPVPTFSHPALAHLVTTPPGARPDIPIL
                     DTKENRPWSGHTPRHA"
BASE COUNT          617 a          979 c          851 g          616 t
ORIGIN      
        1 tcccatgcgg ctcactgatc tgattgcgca cgacgccatc attccttcgt tacgcgccgg
       61 gacaaaaaaa ctcgttctgc aggagttaag cgaaaaggcg gcccgcatct ctggtctgcc
      121 accgcgcgaa atcttcaatg cgctcctgca acgtgaacgc ctcggctcga ctggcaccgg
      181 caacggcgtc gccctgcccc atgcgaaact caagaaatgc cagcgcatct tcgggatttt
      241 cgcgcggctc gaaaagccga tcgactttga ggcgattgat ggcgaacctg tcgatctcgt
      301 gttgatgctt gtggcgccgg aatttgccgg cgcagatcat ctgaaggcgc tcgcgcgcgt
      361 cgcccgcgtc ctgcgcgacg tcaggatcgc agcgaagctg cgcgccgcgc gcgatcccgc
      421 tgcgctctat gccgtgctga ctcaggaacc cacgtccaac gccgcctgaa tccggatttt
      481 ccgggactgg catcacagcc ggatggacgc gacctcaaca tgcgttagat tgccgccatg
      541 ttgagcgatg ccggaaagcc cattgctgtt tcatcggtcc cggccctgct ggagcaggcc
      601 gctagcgcgt ttccagatca taatgcgttg gattttttcg gtcgtaagct gacctacacc
      661 gacctggcgc atcaagtcga ccgcgccgcg cgcggtttcc agcgtctcgg cgtcgtgcac
      721 ggcacgcgag tcggcctctg tctgcccaat acgccctatt tcgtcatcgc gtatttcgcg
      781 gtgctgaaag ctggcggaat cgtcgtcaat ttcgatccgc tccaggtcgc gcgcgagatt
      841 gcgcgccaga tcgaggactc cggcacgacc atcatggtca cgctcgatgt gacgaaaatc
      901 tatcccaaga tcgctgaggc tttggagacg acctcgctgg aaaaagtcgt catctgctcg
      961 ctgtgcgcgg cgctgcccac aggcaagggt ctgctctttc gcctcttcaa ggccagggag
     1021 ctcgccagga ttccgcgcaa cgcgaaacac gttcgtttcg agaaattgtt cgctgaccgc
     1081 acgccgatga cgccagtgac catcaatccg gtgattgatc tcgcagttct gcaatacacc
     1141 ggcggcacca ccggcgtccc gaagggcgcg atgctcagcc atgcgaacct caccgcgaat
     1201 gtcgcgcaga ttatcgatcg cgtgccggtc ctccggccgg gtcaggagag cgcgatcgtc
     1261 ttgctgccgc tcttccacgt cttcgggatg acgacgagca tgaacctctg cgtcgcgctc
     1321 ggtttcgaga tgatattgct acccagtctg gatctgccgc aattgttgaa actgattgtc
     1381 agcaagaagc cgacgctctt ccccggcgtg cccagtcttt ttgcgacatt gagcaacatg
     1441 gcggagcacg gcacgttcga tctgtcgtca atccgcttct gcatatccgg cggcgcgcca
     1501 ttgccgcttg aaacgcggca acgtttcgag gccctgaccg gctgcgaact catcgagggc
     1561 tatggcttga cggaagcttc gcctgtcgtc gccgccaatc cgctcgatgc accgtataag
     1621 aatgaatcgg tcggaccacc gctgaaagaa acgatcgtcg aaattcgctc actcgacgat
     1681 cctatgaaaa tcctccgcgc cggcgaacgt ggcgaaatct gcctgcgcgg gccgcagatc
     1741 atggccggct attggcgcaa cctccaagag acgaaggccg cctttgtcga aggcgccttg
     1801 cgaaccggcg atgtcggcta tctcgatcaa gacggcgacc ttttcctcgt cgaccggatc
     1861 aaggacatca tcatttgcaa cggattcaaa atttacccac gcgcgatcga ggaagcgctt
     1921 gtgcaacatc ccgcggtcga tgaggcggtg gcgataggaa ttgccgacgc ggtgcgtgga
     1981 cacgtgccaa aagctttcgt cacgctcctg cctggcgctg ccacaacgcc agatatgctt
     2041 ctggaatttc tcaaaaccca tttatcccgg ctcgaaatgc cgcgcgtgat tgaaattcgc
     2101 gcggcactcc ccaaaaccaa tatcggcaag atcagccgca aggatctggt tttggaggaa
     2161 gaacgtttgc acgcggtttc ggagtaatgg cgactcggcg cctcaaatcg cagaccaaga
     2221 cctcctcaga agaaagcttg ttgccaccgc gcttcgcgga atttttcacg cgccgcggct
     2281 ggacgccgcg ccaacatcag ctcgcgcttt tgaaacatgc cgaagccggc gaggacgagc
     2341 tgttgatcgc gccgactggc gccggcaaaa cgctcgcggg ttttctgccg acgctcatcg
     2401 atctcgatag gccgcgaaac cgccggggcc tgcacacgct ctacctgtcg ccgctgaaag
     2461 ctctcgcgac tgacgttgcg cgcaatctcg aaattcccat cgccgagatg ggcctcaaca
     2521 tccgggcgga gacgcgtacc ggcgatacag cgatgtcgaa gcgccagcgt aaacgccgcg
     2581 atccgcccga catcctcctg acgacgccgg agcagctcgc acttctgctg gccagcgccg
     2641 acgcacgctt tcttttcgct gaccttaagc gcgtcatcat tgatgaattg cattcgctga
     2701 cagcgtcgaa acgcggcgat cttctgagcc ttgatctcgc ccgcctgcgc gacatcgcgc
     2761 cgcaaatgcg cgccaccgga ttgtccgcga ccgtgcgcga tcccgacgaa ttgcgccgct
     2821 ggctggtgtc gcaatcgcac gatgcattag atcacgatgg ttttggatcg gatcgatcta
     2881 aaaccatgaa cgtgatcgat tctaaaaatt tagagccgga ttcacgcgaa aaaccggtac
     2941 ccactttttc gcatccggct ctagcccacc tcgtcacaac gccgcccggc gccagaccgg
     3001 atattccgat cctcgatacc aaagaaaacc ggccctggtc cggccatacg ccacgacatg
     3061 cga
//
LOCUS       DASWOG010000627         8892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_309723,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000627 DASWOG010000000
VERSION     DASWOG010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309723"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGB93_12860"
     CDS             complement(<1..633)
                     /locus_tag="VGB93_12860"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HEY0147006.1"
                     /translation="MAEMDLLTRSDELYAKVSHRIPAIEWPFFSGDIEAIQRLKRERN
                     AVILAHNYQTPEIYHCVADIVGDSLALAREAVDVDADVIVLAGVHFMAETAKLLNPKK
                     KVLIPDMAAGCSLADSITPENVRELRRRHPGVPVVTYVNTSAAVKAESDICCTSGNAR
                     KIIESLGVPRVIMIPDEYLAKNIAAETGVEIITWKGHCEVHELFGTEEVAQ"
     gene            complement(693..1682)
                     /locus_tag="VGB93_12865"
     CDS             complement(693..1682)
                     /locus_tag="VGB93_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147007.1"
                     /translation="MNTAESATTVPRAASESLQLSAASTSGIAADLVAVLVAVTGGEP
                     RVLTLQDARLLPSGAFELTHRSLQSGLRAWVERQTHHPVGYVEQLYTFADRDRGLGPE
                     QRVISVSYLGLTRERPPGPGDQTSWQDWYLYLPWEDRRVGVPSMIDAVILPALRAFAK
                     AAPSAALRHDRANRIAATFGTDGRPWNEEFVLQRYELLYEAGLIPEARRGEGDAGAAP
                     VHGRSMSLDHRRILATGIARLRAKIKYHPVVFELMPDTFTLLQLQRCVEALAGRLVHK
                     QNFRRLVEQQELVEETGQSSQETGGRPAKLFRFRREVLFERALVGTKLPISRS"
     gene            complement(1755..2153)
                     /locus_tag="VGB93_12870"
     CDS             complement(1755..2153)
                     /locus_tag="VGB93_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0147008.1"
                     /translation="MAAKLGGPITTEARRQRARIAGRRAENWAALYLRLKGYRILARG
                     YCVRGGEIDLIARRGSNIVFVEVKYRPTLIAAMTAIDTWKRQRVSRTARVWLSATPWA
                     AALTLRGDAVYLAPRQWPRHAIVAFALDFD"
     gene            complement(2205..3119)
                     /gene="rsmI"
                     /locus_tag="VGB93_12875"
     CDS             complement(2205..3119)
                     /gene="rsmI"
                     /locus_tag="VGB93_12875"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0147009.1"
                     /translation="MSENHNALEGAPMFTAFGLRAEAVKLSPGLHVVATPIGNLGDIS
                     LRALSTLAAADAVIAEDTRVTKILLAHYGISTPLVAYHEHNAAVMRPHLVARLTNGAA
                     LALVSDAGTPLISDPGYKLVASALAEGLPVTSVPGASAVLTALVVAGLPTDRFFFEGF
                     LPPKSAARRQRIAVLAPIPGTLVFFESQHRVAETLNDLAEVLGPRDAALARELTKVFE
                     TVRRGTLPELAETFAAEPTPKGEIVLLVGPAQDGALEIDAEELDRRLAKALETLSVKE
                     AATVISVETGQPRRKVYARAVELSARNR"
     gene            3348..4607
                     /locus_tag="VGB93_12880"
     CDS             3348..4607
                     /locus_tag="VGB93_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY0147010.1"
                     /translation="MFQRFGPARHFGRRLGAGGGLAFALLGTLALGACEEVNPSASTN
                     NNSDSFSASAPVTPVQTQPLPGAPPGPAGSGSVRVAMILPLTQASGTPSVVGVSLKNA
                     ADLAVSESGGSAVTFSYMDDHSTPDGAKTAAQQAITENDDIILGPLFSPSVRAVGEMA
                     QAAHKPVIAFSTDTSTAAPGVYLLSFLIENYVDRIVDYAASQGKKSFAALIPNNDYGR
                     VASAEFQQIAAQRGLRVVEIENYTPQTLASAVGKVAAFGNQIDALFIPEQADAMPAMA
                     HALRSAGLDSHKVQILGTGLWNDARVLGLAPLQGAWFAAPDNGGFQAFAQRYRAKYGS
                     DPTRIATLGYDAASLVIALAKRGGGDKFSDNVLTNSGGFNGADGVFRFKPNGQNERAL
                     AIFEIENGSAVALSQAPRSFAGRGSAT"
     gene            complement(4609..5772)
                     /gene="hemW"
                     /locus_tag="VGB93_12885"
     CDS             complement(4609..5772)
                     /gene="hemW"
                     /locus_tag="VGB93_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0147011.1"
                     /translation="MARQTASDPGFGVYVHWPFCLSKCPYCDFNSHVRSQAVDERRFV
                     AAIAAELAHRASLTKGRTVRSVFFGGGTPSLMQAKSVAGVLEAIAKHWPMAPDVEVTL
                     EANPSSVEAARFREYRAAGVNRVSIGVQSFNDADLKALGRLHNAAEASNAIDVAAQVF
                     DRYSFDLIYARPNQTVKAWEAELKTALVRVRDHISLYQLTIEPDTMFERLHNAGKLSV
                     PDSDLGRAFWDATQELTAMASLPAYEVSNHARRGAESRHNLIYWRYGEYAGVGPGAHG
                     RIITPKGRRAHATERHPEMWLTVVEADGHALVEDELLSTEEQGDEFLLMGLRLSEGIE
                     PARFEAVAGRRLDPGRIASLLADGMVEMTAAGRLRVSPEGFPVLDAVVADLAA"
     gene            6073..7410
                     /locus_tag="VGB93_12890"
     CDS             6073..7410
                     /locus_tag="VGB93_12890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0147012.1"
                     /translation="MAGTRQSHIKAAARGDILLVNAFQKQGGAARAAYRIFEGIKRFY
                     PNAHYLTLFRDDARSDISGVRRRSLLGWLADRLSAIESRPVRKYRSQREPRFTPAFWA
                     NPLRIPLARLKPRLVHLHWLGAGVASLDELARIDVPVIWTLHDAWPFTGGCHYTGTCD
                     GYQRHCGTCPQLDSDEIGDLSHIVWQRKQRIYKKIAPVIVAPSHWLAEIAGQSSLLAG
                     RRIEVIANGLDLTLFKPADRDRARAHFGLPAEETILLFGAHQLTDPRKGGDLLRAALD
                     RIDFPCHLVTFGSGALSLASTKYVTVQAVAPVDDDAELALLYSAADVFVCPSREDNLP
                     NMVAEALACATPCVAFEINGIPEMICHERNGFLAQPFAVDELVAGIRWVAQHPAPQAL
                     RDAARRKAVESFDRDKMAERYAALYSELLPVPAAPRAAMDCVYSVAIDAAYPR"
     gene            complement(7361..8335)
                     /locus_tag="VGB93_12895"
     CDS             complement(7361..8335)
                     /locus_tag="VGB93_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0147013.1"
                     /translation="MSRSKTKANSVPDSRVSVGSLQRTTLILPETQWRKKPTLIEPAD
                     KRPPRMSLTHWSTPATAFPYEVSTDAPGDSHLLTFSFTTSRGEFSFEGVNPNQGHIEA
                     SSILITGPNRGVFRGIFFRPAQFFRIYLPHGLIEECYAELTTHRPPSDIILFEPQILS
                     DRIVRQLTMSLVGIDEDGSPFGPTFLDSIGLALTSRLVALHLNRRVSLLAAVRTPLAP
                     WRLQRVVDYVESHLSDRISLSDLSEAAGLSRMHFAAQFRLATGVSPHAYIIKRKVERA
                     EQLLLDRAFSIVDIALLLGFSSQAHFTQVFKKIIGDTPHRWRQNIRNP"
     gene            complement(8602..>8892)
                     /locus_tag="VGB93_12900"
     CDS             complement(8602..>8892)
                     /locus_tag="VGB93_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0147014.1"
                     /translation="YIEANIETDISVEALADVAALSVFHFTRLFTAAMGQPPRKYVSR
                     RRLETAMAMLARDKFSLNEIAHKSRFSSQAAFSRAFRRATGMTPGEYRRLVR"
BASE COUNT         1665 a         2671 c         2803 g         1753 t
ORIGIN      
        1 ttgcgcgact tcctcggtgc cgaaaagctc gtgcacttcg caatggccct tccaggtgat
       61 gatctcgacg cccgtctcgg cggcgatgtt cttcgccaga tattcgtccg ggatcatgat
      121 cacgcgcggc acgccgagcg actcgatgat cttgcgcgcg ttgccggagg tacaacagat
      181 gtcggattca gccttcacgg cggccgatgt gttgacataa gtgacgacgg gcacgccggg
      241 atggcgacgc cgcagttcgc gcacattctc cggcgtgatc gaatccgcca gcgaacagcc
      301 ggcggccatg tccgggatca gcactttctt cttgggattg aggagttttg cggtctcggc
      361 catgaaatgg acaccggcga gcacgatcac gtcggcatcg acatccaccg cctcgcgggc
      421 aagcgccagg ctgtcgccga cgatatcggc gacgcaatga tagatttccg gcgtctggta
      481 attatgcgcc aggatcacgg cattgcgctc gcgtttcagc cgctggatcg cctcgatgtc
      541 gccggagaag aacggccatt cgattgccgg aatccgatgg ctcaccttgg cgtagagctc
      601 atcgctcctt gtcagcaaat ccatctcggc catcaaatta tactccctct gagcattact
      661 gagtaatatg ctggggttga gcataacgtt tgtcaagacc gagaaatggg cagtttcgtt
      721 ccgacgaggg cgcgctcaaa caaaacttcc cggcggaagc ggaagagttt tgctggccgc
      781 ccgcccgtct cctggctgga ttgtcctgtt tcttcaacga gttcctgctg ctcaaccaag
      841 cggcggaaat tctgtttatg gacgagccgg ccagcaaggg cctcgacgca gcgttgcagc
      901 tgcagcaacg taaaagtgtc gggcatcagc tcgaacacga ccggatgata tttgatcttg
      961 gcccgcagcc gggcaattcc cgttgccaga atgcggcggt gatcgaggct catgctgcgg
     1021 ccatggaccg gcgcggcgcc cgcatccccc tcgccgcgcc gcgcttcagg gatcaggccg
     1081 gcttcgtaga gcaattcgta gcgctggagc acgaattcct cgttccacgg gcggccgtcg
     1141 gtgccgaagg tcgcggcgat ccgattggcg cgatcgtgcc gcaaggcggc gctgggcgcg
     1201 gctttggcaa aagcgcgcag ggcgggcagg atcaccgcgt cgatcataga gggaacgccg
     1261 acacggcggt cttcccacgg cagatagaga taccaatcct gccagctcgt ctggtcgcca
     1321 ggaccgggcg ggcgctcgcg ggtcaggccg agatagctga cagagatgac gcgctgttcg
     1381 ggcccaaggc cgcggtcgcg atccgcgaag gtataaagct gttcgacata gccgacggga
     1441 tgatgcgtct gccgttcgac ccaggcgcgc agacccgatt gcagggaacg gtgcgtcagt
     1501 tcgaacgcgc cggagggcag caggcgggcg tcctgcaagg tcagcacacg cggctcgccg
     1561 cccgtcacgg cgacgaggac ggcgaccaga tcagccgcga taccggacgt cgaagcggca
     1621 ctcagctgga gtgactcgct ggccgcgcgc ggaaccgttg tagcggattc ggccgtgttc
     1681 attcccatct tcctcgccgc cgaaatctgt ggcggctttt cgccgaatgt ctaaggcgcg
     1741 gatgcaacaa ttattcaatc gaaatcgagc gcgaaggcga cgatagcatg gcgtggccat
     1801 tggcgcgggg cgagatagac agcgtcgccg cgcagcgtca aggccgccgc ccagggggtg
     1861 gctgagagcc agacgcgggc ggtgcgcgag acgcgctggc gtttccaggt atcgatggcc
     1921 gtcatcgccg cgatgagtgt tgggcgatat ttcacttcga cgaagacgat gttcgatccg
     1981 cgccgcgcga tgagatcgat ttcgccgccg cgcacgcaat agccccgcgc gaggatccgg
     2041 taacctttga gccgcagata gagtgcggcc cagttctcag cgcggcggcc ggcaatacgg
     2101 gcgcgctggc gacgggcttc ggttgttatc ggtccgccga gtttggcagc catgatcgct
     2161 ctcagttcaa ttcaccccga atttgcgcgc gtttacgctt tgcttcaacg gttgcgggcg
     2221 gagagttcca cagcccgcgc gtaaactttg cgtcgtggtt gaccggtttc gacggaaatg
     2281 acggtcgccg cttccttgac cgacagggtt tcgagcgcct tggcgagcct gcggtcgagt
     2341 tcttccgcat cgatctcaag cgcgccgtcc tgcgctggcc cgacaagaag gacgatttcg
     2401 ccctttggcg ttggttcggc agcaaaagtt tccgcaagct ccggcagcgt gccgcgccgg
     2461 accgtttcga aaaccttggt cagctcgcgc gccaacgccg cgtcgcgcgg tcccagaacc
     2521 tccgccagat cgttgagtgt ttcagcaacg cggtgctgcg attcgaaaaa aacgagcgtg
     2581 ccggggatcg gggcaagcac tgcgatccgc tgccgccggg ccgcagattt tggcggtagg
     2641 aaaccctcga agaaaaaccg gtcggtcggc agaccggcaa cgacgagggc tgtgagtacg
     2701 gctgaagcgc ccggaacgga ggtcacgggc aggccttcgg ccaaagccga ggcgacgagc
     2761 ttgtagccag gatcggagat gagtggcgtg ccggcatccg acacaagcgc cagcgccgcg
     2821 ccattggtaa gccgggcaac gagatgcggc cgcatcaccg cggcgttatg ctcgtgatag
     2881 gcgacgagcg gcgtcgagat gccgtaatgg gcgaggagaa tcttcgtgac ccgcgtatct
     2941 tccgcaatca ctgcgtcggc cgccgcaagc gttgagagcg cccgcaacga aatatcgcca
     3001 aggttgccga tcggggtcgc gacgacatgg agtccgggcg acaatttcac cgcttccgcc
     3061 cgtaagccga aggcggtgaa catgggggcg ccctctaggg cgttatgatt ttcgctcatc
     3121 ttcccgccaa tggttgggaa cgggtgttct atatatcgtc ccaatgttcc gtatatagaa
     3181 cgcgccgaaa gctcgcaagc caccgaggct cggcgtccaa tttccgagcc gcctcaaatc
     3241 agatataatg tgtttctagt taaactgtct tggcttatgc ggccccgccg cgagccgacg
     3301 aattcctgca acgccgcaat agatattgcg cctggagggg gtctttcttg tttcagaggt
     3361 tcgggcctgc caggcatttc ggccggcgcc ttggcgccgg gggtggtctt gcgttcgcgc
     3421 tgctcgggac gctcgctttg ggcgcctgcg aggaggtcaa tcccagcgcc tcgaccaata
     3481 acaattcgga ctcgttttcg gcttcggcgc cggtcactcc ggtccagacc cagccgcttc
     3541 ccggtgcacc gccggggcca gcgggcagcg gcagcgtgcg cgtcgcaatg atcctgccgc
     3601 tgacgcaggc gtcgggtacc ccgagcgttg tcggcgtatc gctcaaaaat gccgccgatc
     3661 ttgcggtctc ggaaagcggc ggcagtgcgg tgacattttc ctatatggac gatcattcga
     3721 cgcccgacgg cgccaagacg gcggcgcaac aggcaatcac cgaaaacgac gacattattc
     3781 tcgggccgct tttttcgccg agcgtccgcg cggtcggcga gatggcgcag gcggcgcaca
     3841 agccggtcat cgccttctcg accgacacgt cgaccgctgc gcccggcgtc taccttttgt
     3901 cgttcctgat cgagaattac gtcgatcgca tcgtcgatta tgcggcgagc cagggcaaga
     3961 aatccttcgc cgcattgatc ccgaataacg attacggccg cgtcgcctcg gccgagttcc
     4021 agcagattgc cgcgcagcgc gggctacgtg tcgtcgagat cgagaattat acgccgcaga
     4081 cgcttgcgag cgcggtcggt aaggtcgcgg ccttcggcaa tcagatcgac gcgctgttca
     4141 tccctgaaca ggccgatgcg atgccagcca tggcccatgc gctgcgttcc gccgggctcg
     4201 attcgcacaa ggtgcagatc cttggcacgg gcttgtggaa cgatgcgcgc gtgctcggcc
     4261 tcgcgccttt gcaaggcgct tggttcgcgg cgccggacaa tggtggcttc caggctttcg
     4321 cgcagcgcta tcgcgccaaa tacggcagcg accctacgcg gatcgcgacg ctcggttatg
     4381 acgccgcatc tctcgttatt gctttggcga agcgcggtgg cggcgacaag ttttccgaca
     4441 acgtgctgac gaattcaggc ggcttcaacg gcgccgacgg tgtcttccgc ttcaagccga
     4501 acgggcagaa cgaacgcgcg ctggcgatct tcgagatcga gaatggcagc gccgtcgcat
     4561 tgagccaggc gccgcgtagt tttgcggggc gcggctcggc gacctgattt aagccgccaa
     4621 atccgccacg acggcgtcga gcaccggaaa accttcgggg ctgacgcgta gccgtccggc
     4681 ggcggtcatc tccaccatcc cgtcggcgag gagcgaggcg atacggccgg gatcgagtcg
     4741 gcgtcctgcg accgcctcga aacgagccgg ctcgataccc tccgaaagcc gcagccccat
     4801 cagcaggaat tcgtcgccct gttcctcggt cgacagcaat tcgtcctcga cgagagcatg
     4861 gccgtccgcc tcgacgacgg tgagccacat ttcgggatgg cgctcagttg cgtgcgcccg
     4921 gcgcccctta ggcgtgatga tccggccatg tgcgccggga ccgacgccgg catattcgcc
     4981 atagcgccaa tagatcagat tatgacggct ctcggcgccg cggcgcgcgt ggttcgagac
     5041 ctcataggcc gggaggctcg ccatcgcggt cagttcctgc gtcgcatccc agaaggcgcg
     5101 accaagatcg gaatccggga cgctgagttt tccggcgttg tgaagccgct cgaacatcgt
     5161 gtcgggttcg atggtgagct gatacagcga aatatggtcg cgcacacgga cgagcgccgt
     5221 cttcagttcc gcttcccagg ctttcacggt ctggttgggc cgggcataga tcagatcgaa
     5281 tgaatagcgg tcaaaaactt gcgctgcgac atcgatagca ttgctcgcct cggcggcatt
     5341 atgcagccgg ccgagcgcct tgaggtcggc atcgttgaaa gattgcacgc cgatcgagac
     5401 gcgattgacg cccgcggcgc ggtactcgcg aaagcgcgct gcctcgacgc tcgacggatt
     5461 ggcttcgagc gtgacttcga cgtctggcgc catcggccaa tgtttggcga tggcttccag
     5521 cacccctgcg acgcttttcg cctgcatcag cgaaggcgtc ccgccgccga agaagaccga
     5581 gcgcacggtg cggcccttgg tgaggctggc gcgatgggca agttcagccg cgatggcggc
     5641 aacaaaccgc cgctcatcca ccgcctgcga gcggacgtgg ctgttgaaat cgcaatacgg
     5701 gcatttcgaa aggcagaacg gccagtggac gtaaaccccg aagcccggat cggaagcggt
     5761 ttggcgtgcc atgccgcctt atgtcacgag ctagtgattc ggtcatcagg gcaacggcgg
     5821 ctacgcacat tttgttcggc gcgatggtga aacggcgttt ttcgggcaga atttgcgttt
     5881 tttccagaaa cggcgtccta aaactgctcg ccacagagcg tatatggcgc ccgtagctca
     5941 tccataggca ggtttgaagt tcgggaggcc ggtcgcggat gctaacagct gctgccgcgc
     6001 acctttcggc ctacatagtt ctggcgtaat ggcgcgcaga atgggcgcgt ctttgagata
     6061 gatctcctcg atatggccgg cacccgacaa tcgcacatca aagcggcagc acgcggcgat
     6121 atcctcctcg tcaacgcgtt ccagaagcag ggcggtgccg cccgagctgc ttatcgcatc
     6181 ttcgaaggca tcaagcgttt ttatccgaac gcgcattatc tcacgctttt cagggacgat
     6241 gcccgtagcg atatttccgg ggttcgccgc cgctcgctgc ttggctggct tgccgatcgt
     6301 ttgtcggcga tcgagtcgcg cccggtgcgg aaataccgca gccaaagaga gccgcgcttc
     6361 acgccggcgt tctgggccaa tccgctgcgc attccgctcg cacgtttgaa gccacgtctg
     6421 gtacatttgc attggcttgg cgcgggcgtc gcgtcactcg acgagcttgc gcgtatcgac
     6481 gtaccagtga tctggacttt gcacgacgcg tggcctttca cgggcggctg tcattatacg
     6541 ggcacctgtg acggctacca gcggcattgc ggcacgtgtc cgcaacttga tagcgacgaa
     6601 atcggtgatc tctcccacat tgtctggcag cgcaaacagc ggatctataa aaagatcgct
     6661 cctgtgattg ttgcgccgag ccattggctg gccgagattg ccggacaaag cagcctgctt
     6721 gccgggcgcc gcatcgaggt gatcgcgaac gggctcgatc tcacgctttt caaacctgcg
     6781 gatcgcgatc gtgcgcgcgc gcattttggc ctgccggcgg aggaaacaat tctgctcttc
     6841 ggcgcgcatc agctaaccga tccgcgcaaa ggcggcgacc tcttgcgggc cgcgctggac
     6901 cgcatcgact ttccctgcca cctggtgacg ttcgggtcgg gggcgctttc gctcgcatcg
     6961 acgaaatatg tgacggtcca agcggtagcg cctgtggacg acgatgccga attggctttg
     7021 ctctattccg cagcggacgt tttcgtctgc ccatcgcgag aagacaatct gccaaacatg
     7081 gtggcggaag cactcgcctg cgcgacgccg tgtgttgcct tcgaaatcaa cgggattcca
     7141 gaaatgatct gtcacgagag gaacggcttt ttggcgcaac ctttcgctgt cgatgaactc
     7201 gtcgccggga tcagatgggt tgcccagcac ccggcgccgc aggccttgcg cgatgcggca
     7261 cggcgcaagg ccgtcgaaag cttcgatcgg gacaaaatgg ctgaacgcta tgccgcgctc
     7321 tacagcgaat tgctacccgt gcccgccgcg ccacgtgcgg ctatggattg cgtatattct
     7381 gtcgccatcg atgcggcgta tccccgataa ttttcttaaa gacctgcgtg aaatgcgcct
     7441 ggctggaaaa gcccagcagc agcgcaatgt cgacgattga aaatgcccga tcgagaagca
     7501 attgttcggc gcgttcgacc ttgcgtttga tgatataggc gtgtggtgaa acacctgtcg
     7561 cgagccggaa ttgtgccgcg aaatgcatac ggctcagccc cgcggcctcg ctcagatccg
     7621 acagcgatat cctgtctgaa aggtgcgact cgacataatc caccacccgc tgcagccgcc
     7681 acggcgccaa aggcgtcctc acggcggcca acaggctcac gcggcgattc agatgcagcg
     7741 cgacgaggcg cgacgtcagg gcgaggccga ttgaatcaag aaacgtgggt ccgaaggggc
     7801 tgccgtcttc atcaattccc acgaggctca tggtgagctg acggacgatc ctgtcgctca
     7861 atatctgtgg ttcaaacagg atgatatctg aggggggcct gtgcgttgtg agctcagcgt
     7921 agcattcttc gatcaggccg tgcggcaggt aaatcctgaa aaactgcgcc ggcctgaaaa
     7981 atatcccgcg aaagacgcct ctgttcggtc cggtgatcag gatgctcgag gcttcgatat
     8041 ggccctggtt cggattgacg ccctcgaagc tgaattcgcc gcgtgaagtt gtgaaagaga
     8101 aggtcaggag atgcgagtcg ccgggcgcgt cagtgctgac ttcgtaaggg aatgccgtcg
     8161 ccggtgtcga ccaatgagtc aggctcatgc gcggcggcct tttatccgca ggctcgatga
     8221 gcgtcggctt ctttcgccat tgcgtctctg gcagtatgag ggttgtccgc tgcagacttc
     8281 ccacgctgac gcgactatcg ggaacggagt tggccttggt cttagaccgt gacacttgaa
     8341 taccggtcgg agtcgaggat cattcgagac gattgtttcg aatcgccgtc cagacgcgcc
     8401 gacgttgtca acgacgtccc atgtctgcca tgtcggaagg gcttggatat ttgacgttct
     8461 tgcgagagcc tcccaattgg tttgaaaggc gacgcaagcc ggcgcgatct tatcggagca
     8521 ccacgcgctt cgtttgaccg gcattgccgt tcttttgatc gcacttgcta tttccctcat
     8581 gaagacgcgg gcggatgtcg gctatcggac gagccgtcgg tattcgccag gcgtcattcc
     8641 agttgcgcgg cggaaggcgc gcgagaaggc cgcttgcgat gaaaaacgcg atttgtgggc
     8701 gatctcgttc aaggagaatt tgtcgcgcgc cagcatcgcc attgccgtct caagccggcg
     8761 gcggctgaca tatttgcgcg gcggctgacc catggctgcg gtgaacagcc gtgtaaaatg
     8821 gaagacactc aacgccgcga catcggcaag cgcctcgaca cttatgtccg tttcaatatt
     8881 cgcttcgata ta
//
LOCUS       DASWOG010000628         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_236132,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000628 DASWOG010000000
VERSION     DASWOG010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236132"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..328)
                     /locus_tag="VGB93_12905"
                     /pseudo
     CDS             complement(1..328)
                     /locus_tag="VGB93_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495716.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
     gene            571..1395
                     /locus_tag="VGB93_12910"
     CDS             571..1395
                     /locus_tag="VGB93_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0147015.1"
                     /translation="MLIRYGFNIEIELTQPAAVVTAMDVHSSARGGILRERDFRVSQS
                     VPVGTFTDAQGNVLRRFNAQPGRVNLDLDGVYECDSRPDIIDVSANITPVNDVPLDVL
                     PFLQGSRYCETDLLSDLAWRQFGGIQGGWAKVQAVCDFVHNALYFDYQTARPTRTAAQ
                     ALEEGTGVCRDFTHLAVTFCRCLNIPARYCNGYLGDIGVPPDPAPMDFNAWFEAYLGG
                     RWYTFDPRHNIPRIGRILIARGRDAMDIPMLTSYGPHWLNRFEVTTEEIIRVPVAA"
     gene            complement(1392..2204)
                     /locus_tag="VGB93_12915"
     CDS             complement(1392..2204)
                     /locus_tag="VGB93_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147016.1"
                     /translation="MKLKYRVLCAALALAITSPLAAQQTTANEFVIDLNLPDGTRERV
                     LYAAPAHPQATLVMLPGGAGELGLRRDGDIRHDDNFVVRTRSLWVAQNYAVLIPDTID
                     RENLRGLRSSADYAAVVAALVQLARTKATVPVFLLGTSQGSIAAMNGAAHAPPDTIAG
                     VILTESVSRLGHSGETIFDADPQDVGVPALVVANDDDQCDVAPPQDAPRIAAAMSHSP
                     DVRVIHVSGGIEKSARACGSLSPHGYYGIEAKVVDVISAWIKDHMPTKSRPR"
BASE COUNT          467 a          706 c          720 g          531 t
ORIGIN      
        1 acggcttgcg cggcggcgag aaggcccggg tcgagtgcgt cagtatcggc gtactcctcc
       61 atgatctcct tgccttcatc gaggatgccg ttgatcgcgt cgcaggtctt gccacgcgcc
      121 ggcttgtcga tgatctcgaa atctgttcga ggcgttcaac atggccttcc gtctgctcgc
      181 gatgggtctc gaaagcttgg cgcagctcgc ccgactgggt gctcttcgcc attttcggaa
      241 gcgcccgtag aatctgcttt tccgcaaaat agatgtcgcg cagggtgtca taaaaaagga
      301 cgtttaaatc ttttgcttgt ttgaccatct ggctctccaa aatctcgtga agtcgcggca
      361 tttcgcgcgg tagagcccct gcaattcgga ccgattgggt tcgttccgct cgagttcgtc
      421 aaaggagcga aaaagctcaa agcgatgtcg agataagccc gaaggcgctg gacttgagcg
      481 tgacctttgt tagccggccg cgaccatcac gccgggaacg atttagaaag caaattgttg
      541 aggtgccaac aaattttgtg agaccttgtc atgcttattc gctacggctt caatattgaa
      601 attgaactga cgcaaccggc cgcggtggtc accgcaatgg atgtccattc cagcgcgcgt
      661 ggtggcattt tgcgcgaacg cgattttcgc gtgtcgcagt cggttcccgt ggggacgttc
      721 accgacgcac agggcaatgt gctacgccgt ttcaacgcgc aaccgggccg cgtaaatctc
      781 gacctcgatg gcgtctatga gtgcgatagc cgcccggaca ttatcgacgt gagtgctaac
      841 atcacgccgg tcaacgatgt gccactcgac gttctgccat tcttgcaagg cagccgctat
      901 tgcgagacgg accttctatc cgacctcgcg tggcgccaat tcggcggcat tcagggcggg
      961 tgggcgaagg tgcaggcggt ctgcgatttc gttcacaacg ctctctattt cgattaccag
     1021 actgcgcggc cgacccgcac cgccgctcag gccctggaag agggaacggg cgtatgccgc
     1081 gactttaccc atcttgcggt gacgttctgc cgctgcctga atatcccggc gcgatattgc
     1141 aacggctacc ttggcgatat cggcgttccg cccgatccgg cgccgatgga tttcaatgcc
     1201 tggtttgagg cctatctcgg cggacgctgg tacactttcg atccgcgcca taacatcccg
     1261 cgcatcggcc ggattcttat cgcacggggc cgcgacgcta tggatattcc gatgctgacc
     1321 agctacgggc cgcattggtt gaacaggttc gaggtcacga ccgaagagat catccgtgtc
     1381 ccggtcgcgg cttagcgcgg ccgggatttt gtcggcatgt gatccttgat ccaggcgctg
     1441 ataacatcga cgactttggc ctcgatgcca taatagccgt gtgggctcag agacccgcag
     1501 gcgcgtgcag atttttcaat gccgccgctg acatggatga cacgcacgtc gggcgaatgc
     1561 gacatggccg cggcaatgcg tggcgcatcc tgcggcggcg cgacgtcaca ctgatcgtcg
     1621 tcgtttgcga ccacgagcgc cggcacgcca acatcttgcg ggtcggcatc gaagattgtt
     1681 tcgccgctat gaccgaggcg tgaaacggac tcggtgagga ttacgcccgc aatcgtatcg
     1741 ggcggcgcat gggcggcccc gttcatcgct gcgatcgaac cctggctggt tccgagcaga
     1801 aagaccggaa cggtagcttt cgtccgcgcc aattgcacca aagccgcgac gaccgcagca
     1861 taatccgccg aagagcgcag cccgcgtaaa ttctcgcggt cgatcgtatc gggaatcagc
     1921 acagcataat tttgcgcgac ccacaggctt cgggttcgca ccacgaaatt atcgtcgtgg
     1981 cggatatctc cgtcgcggcg aagtccgagt tcgcccgcgc caccgggcaa catgacgagt
     2041 gtcgcctgcg gatgcgcagg cgccgcgtag aggacacgct cacgtgtgcc gtcgggcaga
     2101 ttcagatcaa tgacgaattc gttcgctgtc gtctgctgcg cggcaagtgg tgatgtgatt
     2161 gcaagagcca atgccgcaca aagaacgcgg tattttagct tcatgggaat tcgtctcttg
     2221 gaggcttgga tattttcggc tggcgtccgc gctcgcatta tccttagatc agggcattcc
     2281 gattcacgga agacggctca tgacacggcg tctcataccg ctcggtgcct tcctgctcat
     2341 gacgagcgct gctgcggcgt caggtcaacg cgaactcgat ctgcatcagc gcgacatcat
     2401 taaacattat gtgctgcgcg aggc
//
LOCUS       DASWOG010000629         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_199577,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000629 DASWOG010000000
VERSION     DASWOG010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199577"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VGB93_12920"
     CDS             complement(<1..922)
                     /locus_tag="VGB93_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0147017.1"
                     /translation="MTDLKRATAELAKKGSLVLASLPDGFDAFCIADLSRALAPTAEE
                     RAVVLVHIARDEQRARAFNETLAFVAPEIEILDFPGWDCQPYDRVSPNAAISARRMTV
                     LSRLARSRTAAGRPRILSTTVNAVLQRVPPREKVAAESFSAAPGNAVNMTSLTEWLQN
                     NGFARASAVRDTGEYAVRGGILDLFAPSMLEPIRLDFFGDTLESIRTFDPETQRTVAP
                     LRAVDLVPVSEAQLTTDSMRRFRQGYVGAFGGGLRGDTLYEAVSEGRRHPGLEHWLPL
                     FYDGLDTLFDYIGGAPLVLDPLTDEAATERL"
     gene            complement(950..1228)
                     /locus_tag="VGB93_12925"
     CDS             complement(950..1228)
                     /locus_tag="VGB93_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY0147018.1"
                     /translation="MTQTDDPRRRRALFRSWHRGMRETDLLMGRFADAEIARLSETEL
                     TDYEALLEAQDRDILGWLTGELEIPSSFDTPVLGRLRQFHTHAGPTHI"
     gene            1338..1850
                     /locus_tag="VGB93_12930"
     CDS             1338..1850
                     /locus_tag="VGB93_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY0147019.1"
                     /translation="MSKRPSHSPWSRPLADLVGAVIDPVLARQGFGESQIVLYWEDIV
                     GERLAAMSEPLALKWPPRGPARTEHAPATLVIRVESGFALELQHLAAIVIERVNGYLG
                     FACIDRIALRQGPVTRRAAVARRRPPSPQAVAAAEAMVDDVSEGPLREALARLGAQII
                     EHSLDPKPAS"
     gene            1951..2577
                     /locus_tag="VGB93_12935"
     CDS             1951..2577
                     /locus_tag="VGB93_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0147020.1"
                     /translation="MIVAVAIGGVSAMQLWPAQAQTVTIPPEKLNARQALPDVVQGKA
                     DAPVTIIEYASMTCPHCARFHAETYPTLLSKYIETGKVRFTLREFPLDPRAAAAFMLA
                     RCSGDGDKRTAVIDLLFNAQQSWAYSDTPIDALAGLMKQTGMGDKDFQACLNDQKLYD
                     KVIKEREDAGKDFAIDATPTFFINGTKYSGEMPVAALTKILDPLVAKK"
     gene            2616..3026
                     /locus_tag="VGB93_12940"
     CDS             2616..3026
                     /locus_tag="VGB93_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEY0147021.1"
                     /translation="MNLLQFLLNVLWIAFGGIWMSVAWVIAGIITAITIIGLPWAPAA
                     FRIAAYTLLPFGSRAVPRAQITGRDDFGTGPLGLLGNILWLVLAGWWLALGHIITAAM
                     WAVTIVGLPFAWAHLKLAGIALWPIGKIIVPANA"
     gene            3161..>4518
                     /locus_tag="VGB93_12945"
     CDS             3161..>4518
                     /locus_tag="VGB93_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0147022.1"
                     /translation="MKFKKLHIAGFKTFAESTDFLIEPGLTGIVGPNGCGKSNLVDAL
                     RWVMGESSSKNMRGAEMDDVIFSGSGNRTGRNMAEVRLTLDNSARTAPAVFNDDEVIE
                     VTRRIERDAGSSFRDNGREVRARDVQLLFADASTGSRSPAMVRQGQISELISVKPQQR
                     RRILEEAAGIAGLYSRRHEAELRLKGAEDNLVRLEDVVKQIEAQIDGLKRQARQATRY
                     RVLATDIKRNEALLALVNFGEANAQVTAAGQKVEDDLKLVADATLHQAETARLQAVAA
                     HELPPLREAEANAGSELQKLVLARETLDGEERRAKARLSELERRIAQMNADLGREQAL
                     IEDAASVLGRLDGEVEELAAFGAGDTDVESAARAKLAEAETALAASEAALGTAQSAFS
                     NVEAQRNALNKNLRDETQRYSQFEAELAKVSTELAALRTEIENASADDLFSAAFKAAS
                     AAA"
BASE COUNT          876 a         1400 c         1438 g          804 t
ORIGIN      
        1 acagacgctc ggtcgcggcc tcgtcggtca gcggatcgag caccagcggc gcgccgccga
       61 tataatcgaa caacgtatcg agcccgtcgt agaacagcgg cagccaatgt tcgaggccgg
      121 gatggcgccg cccctcactc accgcctcgt aaagcgtgtc gccgcgcagg ccgccgccga
      181 aggcgccgac atagccctga cggaagcgtc gcatggagtc ggtcgtcaac tgcgcttcgc
      241 tgaccggcac aagatcgaca gcacgaagcg gagcgacggt gcgttgcgtc tccgggtcga
      301 aggtgcggat cgattccagc gtatcgccga agaaatcgag ccggatcggc tccagcatag
      361 acggcgcgaa aagatcgaga atgccgccgc gcacggcata ttcaccggta tcgcgcacgg
      421 cggacgcgcg cgcaaagcca ttgttctgca gccattccgt caggctggtc atattgacgg
      481 cattgccggg tgccgcagaa aaggattcgg ccgccacttt ttcgcggggc ggcacgcgct
      541 gcaggactgc gttgaccgtc gtcgagagaa tgcgtggccg gccggcagcg gtgcgcgaac
      601 gcgcaagccg cgacagcacg gtcatccgcc gcgccgagat cgctgcattg ggcgacacgc
      661 ggtcgtaggg ctggcaatcc cagcctggaa aatcgagaat ttcgatctcg ggtgctacga
      721 aagccagcgt ttcattgaag gcgcgggcac gctgctcgtc gcgtgcgata tggacaagca
      781 cgacggcgcg ctcttccgcc gtcggcgcga gagcccggct gagatcggca atgcaaaagg
      841 catcgaaccc gtccggcaat gaggccagga cgagcgagcc ttttttggca agttcggcgg
      901 tcgcgcgctt cagatcagtc acgggtcaat aattccagat gccagagcct cagatatggg
      961 tcggcccggc atgggtgtga aactgccgca gccgtccgag caccggcgtg tcaaaggacg
     1021 acgggatttc gagttcccct gtcagccagc cgagaatatc gcggtcctgc gcttcaagaa
     1081 gcgcttcata atcggtcaat tccgtctcgc tgagacgggc gatctcggca tcggcgaaac
     1141 ggcccatcag taagtccgtc tcgcgcatcc cgcgatgcca ggagcggaat aaagcccgcc
     1201 tgcgccgcgg atcgtcagtc tgcgtcatta tcccaaccaa aagtcaccaa gtcgggcttg
     1261 gtagcgcctt tggcggcgta tctcaatctt tgatcccgcc tctttgatcc cgcctctgcc
     1321 gcgggctata aaggcgcatg tcgaaacgtc cgtctcattc gccctggtcg cggccgctgg
     1381 ccgatctcgt cggcgcggtg atcgatccgg tccttgcccg gcagggcttc ggcgagtcgc
     1441 agatcgtcct ttattgggag gatatcgtcg gcgaaaggct cgcggccatg tccgagcctt
     1501 tggcgctgaa atggccgccg cgcggacccg cgcggaccga gcacgcgccg gcaacgctgg
     1561 tgatccgggt cgaaagtggg ttcgcgcttg aattgcagca tcttgccgcg attgtgattg
     1621 aacgcgtcaa tggctacttg ggctttgcct gcatcgaccg gattgccctg cggcaagggc
     1681 ctgtgacgcg ccgggccgcg gttgcgcgaa gacggccgcc atccccccaa gcagtcgccg
     1741 cggccgaagc gatggtcgac gacgtcagcg aaggtccgtt gcgcgaggcg ttggcgcggc
     1801 ttggcgccca gattatcgaa cattcacttg accccaagcc cgcctcgtga tcggaaaccg
     1861 tccgaaagag ctgcccggct tcaatcggaa ttttcgacgg gggagtttat gtctcgatat
     1921 cagcggtctt ccgccttgcg cgcgcttgga atgattgtgg cggtcgccat cggcggcgtt
     1981 tcggccatgc agctttggcc cgcacaagcg caaaccgtga cgatcccgcc cgagaagttg
     2041 aatgcgcggc aggcgctgcc cgatgtcgtc cagggtaaag ctgacgcgcc ggtcacgatc
     2101 atcgaatatg cctcgatgac ctgtccgcat tgcgctcgtt tccatgccga gacctatccg
     2161 acgctgctga gcaaatatat cgagaccggc aaggtgcgtt tcacgctacg cgaattcccg
     2221 ctcgacccgc gcgcggccgc ggccttcatg ctggcacgct gctccggcga tggcgacaag
     2281 cgcaccgccg tgatcgatct cctcttcaat gcgcagcagt cctgggccta ttccgacaca
     2341 ccgatcgacg cgcttgccgg cctgatgaag cagaccggca tgggcgacaa ggatttccag
     2401 gcctgcctca acgatcagaa gctctacgat aaggtcatca aggagcgcga ggacgctggc
     2461 aaggacttcg ccatcgacgc aaccccgacc tttttcatca atggcacgaa atatagcggt
     2521 gaaatgccgg tcgctgcact gaccaaaatc ctcgatccgc tggtcgcaaa gaagtaagat
     2581 agaatgcggg tggcgcccaa gcacaggagg tcgctttgaa cctgctacag tttctcttga
     2641 acgtgctgtg gatcgcgttc ggtggcatct ggatgtcggt cgcctgggtg atcgccggga
     2701 tcatcacggc gatcacgatt atcggcctgc catgggcccc ggccgcattc cgcatcgccg
     2761 cctatacatt attgcccttc ggcagccgag ccgttccgcg cgcccagatt accggccgcg
     2821 acgacttcgg cacgggtccg cttggcctcc tgggcaatat tctgtggctc gtgcttgccg
     2881 gctggtggct cgcgcttggc cacattatca ctgcggcgat gtgggcggtc acaatcgtcg
     2941 gcctgccctt cgcctgggca cacctgaagc ttgccggtat cgcgctctgg ccgatcggca
     3001 agatcattgt cccggcgaac gcctgaccaa tcgggacggc gttgcgcaca gaaaaagcgc
     3061 caccttgcct tgcgttggcg ggcgcccttg cccgtaatcg agagcatttt cgcacaggcg
     3121 gccatgagag attagatgaa agtgaaagtc gcatacggcg atgaaattta agaaacttca
     3181 tatcgccggc ttcaaaacgt ttgccgaatc gacggatttc ctgatcgaac cgggactgac
     3241 gggcatcgtc ggaccgaacg gctgcggcaa gtccaatctc gtcgacgcct tgcgctgggt
     3301 gatgggtgaa agctcgtcga agaatatgcg cggcgccgag atggacgacg tgatcttctc
     3361 cggcagcggc aaccggaccg gccgcaacat ggccgaagtg cgcctgacgc tcgacaacag
     3421 cgcgcgcact gccccggcgg ttttcaacga cgacgaagtg atcgaagtca cccgccgcat
     3481 cgagcgcgat gccggatcga gctttcgcga caacggccgc gaggtccgtg cccgcgacgt
     3541 gcaattactg tttgccgatg cgtccacggg ctcgcgctcg ccggccatgg tgcggcaggg
     3601 acagatcagc gaactcatat ccgtcaagcc gcaacagcgc cgccgcattc tcgaagaggc
     3661 cgccggcatc gccgggctgt actcgcgccg gcatgaggcg gaacttcggc tgaagggggc
     3721 cgaagacaat cttgtgcgtc tcgaagacgt ggtgaagcag atcgaggcgc agatcgatgg
     3781 gctgaagcgt caggcgcggc aggcgacgcg ctatcgtgtc ctcgccaccg acatcaagcg
     3841 taacgaagcg ttgcttgcgc tcgtcaattt cggcgaagcc aatgcgcagg tcactgccgc
     3901 cggacaaaaa gtcgaagacg atctgaagct cgtcgccgac gcgacgttgc atcaggccga
     3961 gacggcgcgt ttgcaggctg tcgcggcgca tgaactgccg cccctgcgcg aagccgaggc
     4021 taatgccggc agcgaattgc agaaactggt cctggcgcgc gaaacgcttg atggcgagga
     4081 gcggcgcgcc aaggcgcggt tgtccgagct tgaacggcgc atcgcacaga tgaatgccga
     4141 ccttggccgc gagcaggcgc ttatcgaaga tgccgcctcg gtgctggggc ggctcgacgg
     4201 cgaagtcgaa gaactggcgg cgttcggcgc cggtgatacg gatgtcgaat ccgccgcgcg
     4261 ggccaaactt gccgaggcgg aaaccgcgct agcggcatcg gaggccgcgc tcggcaccgc
     4321 gcaaagcgcc ttctcgaatg tcgaagcgca gcgcaatgcg ttgaataaaa atctccgcga
     4381 cgagacgcag cgatattcgc aattcgaggc cgagcttgcg aaggtgagca ctgaactcgc
     4441 agccctgcgt accgagatcg aaaatgcgag cgcggatgat ctgttcagcg ccgcgttcaa
     4501 ggcggcaagt gccgccgc
//
LOCUS       DASWOG010000630         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_272660,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000630 DASWOG010000000
VERSION     DASWOG010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272660"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGB93_12950"
     CDS             <1..444
                     /locus_tag="VGB93_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0147023.1"
                     /translation="GDVVATRGDTILKTPRLTVFLAPKAGGTNAPKTSSSDRVRRMEA
                     VGPVTLISKDQVATGDSGIYQKGENKVYLDGNVRLTQGPNVTLGDHIVYDLKTTQAVV
                     TGHVRSLFIPDSDNSTSSTDTIDSAKPKPKRLHKASVRRPESAEQ"
     gene            521..1537
                     /gene="lptB"
                     /locus_tag="VGB93_12955"
     CDS             521..1537
                     /gene="lptB"
                     /locus_tag="VGB93_12955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0147024.1"
                     /translation="MTTWLRGIFGRRNGASALPPVQSAEELLFNSPEGAEAQEALSPD
                     WLPQDLENWGTTWTEPPAALDEVPAPRRESSNAAPPRTAARAAPAHKEAHPPRPDGSL
                     IVQGLSKAYRKRQIIKDVSLMVQRGEAVGLLGPNGAGKTTLFYMITGLIRPDSGLIML
                     DDYDVTALPMYRRARLGIGYLPQEPSIFRGLTVEENIRAVLEITQPDKTKRADELEAL
                     LAEFDIARLRKVPAIALSGGERRRCEIARSLAGHPSFMLLDEPFAGIDPIAIGDIRQL
                     VQHLKRRGIGVLITDHNVRETLDLIDRAYIVHSGGILTEGSPEEIVANADVRRFYLGE
                     SFRM"
     gene            complement(1592..2752)
                     /gene="dapE"
                     /locus_tag="VGB93_12960"
     CDS             complement(1592..2752)
                     /gene="dapE"
                     /locus_tag="VGB93_12960"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0147025.1"
                     /translation="MSPRSETARQLARDLIRCPSVTPQDGGALGLLEERLKPAGFTTH
                     RLRFTETGTPDIDNLYARIGAGAPYLVFAGHTDVVPPGDTAAWRFDPFSGEIADGQVW
                     GRGAADMKGAVAAFAAAALDYAAANPAFKGSIGLLITGDEEGPAVNGTVKLLQWADGQ
                     GERFDHCIVGEPTNTEALGDTIKIGRRGSLTGELIVHGTQGHVAYPQRADNPIPHMLR
                     LLAALTEPSLDEGSQRFDPSHLEVLTVDVGNLATNVIPAKAHARFNIRFNDLWTPETL
                     QAEIARRCAAVTDGGHYELSFHPCNALAFVTEADHFTDLAVEAIAAHTGRIAHFSTSG
                     GTSDARFVRTYCPVIEFGLVGETMHKIDEHVPLADIDTLTAIYREILDRYFA"
     gene            complement(2785..3105)
                     /locus_tag="VGB93_12965"
     CDS             complement(2785..3105)
                     /locus_tag="VGB93_12965"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY0147026.1"
                     /translation="MSSAGRLDRQPFWTGLIVLFIISLVLRLFFLLVFGHHSSVGHVF
                     FLAVELPLLYAFVNISIKRFHDRDKSGWWVLIVLVPLIGELWYLIDAGFLPGTDGPNR
                     YDPT"
     gene            complement(3188..>3686)
                     /locus_tag="VGB93_12970"
     CDS             complement(3188..>3686)
                     /locus_tag="VGB93_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147027.1"
                     /translation="RAFERLMPQPGLSFLYRSDEGAIDAATWWRGALMLTGIFAVLTL
                     GWILIEPFADKSLATTIIGTIVVLTGNLYRLAYGFICVLLLISLYNLSAKRWRNLGRP
                     SSFAGILPLAAAVTAALYWLEPRVDGDLPHYWIVAADIVLAGIFLWSVIELGDIRACI
                     TALRR"
BASE COUNT          796 a         1089 c         1148 g          653 t
ORIGIN      
        1 ggcgatgtcg ttgcgacgcg cggcgatacg attttgaaga cgccgaggct cacggtcttt
       61 ctcgcgccca aagcgggggg cacgaatgcg ccgaagacgt cctcgtcgga tcgcgtccgc
      121 cgcatggagg cggtcggccc ggtgaccttg atctccaagg atcaggtcgc aaccggcgat
      181 tccggcattt atcagaaggg cgagaacaag gtttatcttg acggcaacgt ccggctcacc
      241 cagggaccga atgtgacgct gggtgaccat atcgtctatg atctcaagac gacacaggct
      301 gtggtgacag gccatgtgcg tagcctgttt attcctgaca gcgacaacag cacgagcagc
      361 accgatacaa ttgattccgc gaagccaaag ccgaaaagat tgcataaggc cagcgttcgc
      421 cgcccggagt ctgcggaaca atgacgacag gttcgggggg cgactttcgt gtaatcctaa
      481 caccatggct acaacgggct cagccgcgca tgaggtgggc atgacgacgt ggctgcgggg
      541 cattttcggc cgccgcaatg gcgcgtccgc gctgccgccg gtgcagagcg cggaggaatt
      601 gcttttcaac agtccggaag gcgctgaagc gcaagaggca ctttcgcccg actggctgcc
      661 gcaagacctc gaaaactggg gcacgacatg gaccgagccg cctgcggcgc ttgatgaagt
      721 gcctgcgccg cgccgcgagt cgtcgaatgc agcgccgccg cggactgcgg cgcgtgccgc
      781 accggcgcac aaggaggctc accccccgcg gccggacggc tcactcatcg ttcagggatt
      841 gagcaaggct tatcgcaaac gccagatcat caaagatgtc agcctgatgg tccagcgcgg
      901 cgaagcggtt ggcctgctgg ggccgaacgg tgccggcaaa acgacgctgt tttatatgat
      961 caccggtctc atccggcccg actccggact gatcatgctc gacgattacg acgtgaccgc
     1021 gctgcccatg tatcgccgcg cccggctcgg tatcggctat cttccccagg agccgtcgat
     1081 ctttcgcggc ctgacggtcg aagaaaatat tcgtgcggtg cttgaaatca cgcagccgga
     1141 caagacgaag cgcgctgacg aactcgaggc gcttctcgcg gaattcgaca ttgcgcggtt
     1201 gcgtaaagtg cctgcgattg cgctctctgg tggtgagcgc cgccgctgcg aaatcgcccg
     1261 ctcgctcgcc ggccatccct ccttcatgct gctcgatgaa ccgttcgctg gcatcgatcc
     1321 gattgccatc ggcgacatcc gccagcttgt ccagcatttg aaacggcgtg gcatcggcgt
     1381 gctcatcacc gaccataatg tccgcgagac tctcgatctg attgatcgtg cgtatatcgt
     1441 ccacagcggc ggcattctga ccgaaggctc gcccgaggag atcgtcgcca atgccgacgt
     1501 gcggcgcttc tatctcggcg agagcttcag gatgtagcat caaaccggaa agcaggagcc
     1561 gctttcggtt tcattccatg caccagaata atcacgcgaa atagcgatcc agtatttcgc
     1621 ggtagatcgc ggtcagcgta tcgatatcgg cgagcggcac atgctcgtcg atcttgtgca
     1681 tcgtctcacc gacgagaccg aattcgatga ccgggcaata ggtgcgaacg aagcgcgcgt
     1741 cggaggtacc gcccgaggtc gagaaatgcg cgatgcgccc cgtgtgtgca gcaatagcct
     1801 cgacggcaag atcggtgaaa tgatcggctt cggtgacgaa ggcgagcgca ttacagggat
     1861 ggaagctcag ctcgtagtgg ccgccatccg tcacggccgc gcagcgccgg gcaatctcgg
     1921 cttgcaaagt ctcgggcgtc caaagatcgt tgaagcggat attgaagcgc gcgtgcgcct
     1981 ttgccggaat aacattcgtt gccagattgc cgacatcgac ggtgagaact tcgagatggc
     2041 tcggatcgaa acgctgtgag ccctcgtcga gcgacggttc ggtcaaagcc gcgagcaggc
     2101 gcagcatatg cgggatggga ttgtcggcgc gctgaggata agcaacgtgg ccttgcgtgc
     2161 catggacgat caattcgcct gtgagcgatc cgcgccgacc aatcttgatc gtatcgccga
     2221 gcgcttcagt attggtcggc tcaccgacaa tgcaatgatc gaaacgctcg ccttgtccgt
     2281 ctgcccattg cagcaatttc accgtgccgt tgacggcggg tccttcttca tcgccggtga
     2341 tgagaaggcc gatcgagcct ttgaaggccg gatttgcggc ggcataatcg agcgcggcgg
     2401 cggcgaaggc cgcgacagcg cccttcatgt cggcggcgcc acggccccat acttgaccgt
     2461 cggctatctc gcccgagaag gggtcgaaac gccaggctgc cgtgtcaccc ggcggcacca
     2521 catcggtatg cccggcgaac acgaggtaag gcgcgcccgc cccgatccgc gcgtaaagat
     2581 tgtcgatgtc cggcgtgcct gtctctgtga accgcagccg gtgcgtcgtg aagcctgccg
     2641 gcttgaggcg ttcttcaagc aggccaagcg cgccgccgtc ctgcggcgtc acggaagggc
     2701 agcggatcaa atcgcgcgca agctggcgcg cggtttcgga gcgtggcgac atgcgaaact
     2761 tagtggaagc gacggcaacc gccgtcaagt cggatcgtag cgattgggtc catcggtgcc
     2821 gggcaggaaa cccgcgtcga tcagatacca gagttcgccg atgagcggga ccagcacgat
     2881 cagcacccac cagccggact tgtcgcggtc gtggaagcgc ttgatgctga tgtttacaaa
     2941 agcgtagagc agcggcagtt cgacggcgag aaagaagaca tgaccgacgg agctatggtg
     3001 cccgaagacc agcagaaaga acagccgcaa gacgagagag ataatgaaaa ggacaatcag
     3061 cccggtccag aacggctggc ggtcgagccg gcccgcactg ctcaagaaaa gatctcccca
     3121 atccatcgtt ccctccaaat tttctcagaa gttcaatgcg tcacgttcgc aattcaccgc
     3181 aaaagggtca acgccgcagt gccgtaatgc acgcacgaat atcgccgagt tctatgacac
     3241 tccaaaggaa aataccggcc agaacaatat cggcggcgac gatccaataa tgcggcaggt
     3301 ccccgtcgac gcgtggctcg agccaataga gcgccgcggt gacggcggca gccaaaggca
     3361 ggatgccagc gaaactcgac gggcggccga gattacgcca gcgcttggcg ctaagattat
     3421 agaggctaat cagcagcagc acgcagatga agccataggc aagacgatag agattgccgg
     3481 tcaatacgac gatcgtaccg atgatcgtgg tcgccaggct tttgtcggca aatggttcga
     3541 tcaggatcca gccgagcgtg agcacggcga aaatgccggt gagcatcaag gcgccgcgcc
     3601 accaggtcgc cgcatcgatc gcgccttcgt cggagcgata gagaaaagag agacccggct
     3661 gtggcatcaa tctctcaaaa gctcgt
//
LOCUS       DASWOG010000631        10045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_289950,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000631 DASWOG010000000
VERSION     DASWOG010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289950"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGB93_12975"
     CDS             complement(<1..544)
                     /locus_tag="VGB93_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0147028.1"
                     /translation="MVFSACHSPARSNLLKKLDALIIGAGPAGLTTAYSLAKAGKSVL
                     VIEQDPDYVGGISRTAHYKNYLFDIGGHRFFSKSKEVVDLWNEILPDDFIERPRLSRI
                     YYRDKFYSYPLKAFEALSNLGVFTSALCVGSYAYAKLNPVEDPKTFHEWVRNQFGERL
                     FSIFFKTYTEKVWGMSCDEIS"
     gene            complement(781..1263)
                     /locus_tag="VGB93_12980"
     CDS             complement(781..1263)
                     /locus_tag="VGB93_12980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0147029.1"
                     /translation="MVDLGCSDFTIGARLRPYCGNYIACDIVEPLIEWNKQRYKGLNV
                     DFRVLDLSDGEIPSGDVLFVRQVLQHLSNSHIERALAQLSVKFKYLIITEHLPTSGRF
                     THNLDKPAGASVRTGLNSGVVLTSPPFNLKVGEDIVLCETPEGSGIIRTNVYVIPAPR
                     "
     gene            complement(1412..3460)
                     /gene="rpoD"
                     /locus_tag="VGB93_12985"
     CDS             complement(1412..3460)
                     /gene="rpoD"
                     /locus_tag="VGB93_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY0147030.1"
                     /translation="MAKKDNEKTEAEGTAAVETSDSPLLDMSDAAVKRMIKLAKKRGF
                     VTYRELNAVLPSEEVNSEQIEDILAMLNEMGINVIEAEEADEAEAEEEEEEAEGGELV
                     EMTQSKAVATKTSEPADRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREAMIAG
                     LCESPLTFQAIIIWRDELNEGKVLLRDIIDLEATYAGPDGKNTPKIDMTAPGAQEALA
                     AQRAPATAPINPPAGLRPAGDGDDEAEPPISENFDEEDDMENSVSLSAMESELKPKVL
                     ETFDRIADAYKKLNKLQVQNVESKLKNETLTPAQERRYKTLKKEIVVDVKSLSLNQNR
                     IDALVEQLYDINRKLIGLETKLLRRAEESGVSREDFLKNYHNSELDPKWLLRVSKLGG
                     RGWKDFTARGKDHIREIRTDIHDLSKETGVETQEFRKIVQVVQKGEREARQAKKEMVE
                     ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
                     TRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKV
                     LKIAKEPISLETPIGDEEDSHLGDFIEDKNAILPIDAAIQSNLRETTTRVLASLTPRE
                     ERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDN
                     "
     gene            complement(3798..5738)
                     /gene="dnaG"
                     /locus_tag="VGB93_12990"
     CDS             complement(3798..5738)
                     /gene="dnaG"
                     /locus_tag="VGB93_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0147031.1"
                     /translation="MRFPASFLDDLKARLPVSEVVRRRVKLTKAGREWRGLSPFNAER
                     TPSFYVNDQKMAWFDHSANKNGNIFDFLMETEGLSFPEAVERLAQDAGLPLPRATPEA
                     EAQEKQRASLHEVLEAAAAFFVATLASGAGVKARDYLAGRSLTPALQSEFRIGYAPPD
                     KFALRDHLASKGIQAEAMIEAGLLVHGDEIAAPYDRFRDRVMFPIADRNGRVIAFGGR
                     ALSKDVQAKYLNSPETVLFRKGAVLYNHHRARKAAHDKGSVIAVEGYVDVIAMSAAGF
                     PQAVAACGTALTLDQCDLLWKMAEEPILCFDGDRAGRKAAFRAIDLALPLIGVGRSLR
                     FALLPEGQDPDDLARSGGSAAIGAVIEAALPLVDLIWQRETEADVLATPERRAALEHR
                     LLALCGEIRDETLRRHYQAEFRERLGGLFQRRRPDNAAGFSARNRQPQTERREFRRGR
                     FAPAEPQAYASLPLSVGADLARSPFLRSRVADLSARESLIFLILLNHPGLLDPYGDAF
                     AELDLGANEALAVRKRVLDLWAHGPLPADELRAGLATAGFDAFLRRLDASAHAILWIV
                     SPEAADSDAEEALKQALALHHKMRALNSELHSAQDALAQDASEVNLARLLDIREQLSA
                     LTGTEAMIEGFGMASGRQSGMA"
     gene            5839..6651
                     /gene="panB"
                     /locus_tag="VGB93_12995"
     CDS             5839..6651
                     /gene="panB"
                     /locus_tag="VGB93_12995"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0147032.1"
                     /translation="MDIKARFFEMKRQGRQIAVLTGYDFPTAKAEAEAGVDLILVGDS
                     VGTNLLGYASEQEVQLADIAHHVRAVRRGAPATPILADLPYRTYEMPEMAAASARALR
                     DAGADMVKFEGAHPEIAAAIVSAGIPCCGHLGLEPQTHLDKKLRGRSAAEATQLVRDA
                     IALDKAGIAMLVLELIPEVVAARVTQAINAPTIGIGAGRYTNGQVLVITDLLGLTGAS
                     FRHNRRYAELGPLIATAMTSYVRDVRDHAFPTEANTFAMPEEALLAFENGGG"
     gene            6711..7055
                     /locus_tag="VGB93_13000"
     CDS             6711..7055
                     /locus_tag="VGB93_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEY0147033.1"
                     /translation="MELIYKIAPAELWHQAELRGVFSGSPADEADGFIHLSTSAQVRG
                     TAARHFDGQYNLLLVGLDPKLLGPKLRYEAASGGALFPHLYAPLKLDAVVSVAALPLG
                     PDGSHQLDEKFP"
     gene            7052..8164
                     /locus_tag="VGB93_13005"
     CDS             7052..8164
                     /locus_tag="VGB93_13005"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0147034.1"
                     /translation="MSRPHLLTAAAHAIALRLPPETAHRLAVCGLGLLPMPAAAPDDP
                     RLAVKAFGLDFPNPLGLAAGFDKNGEAVDAILRLGFGFVEAGTVTPRPQPGNSRPRIF
                     RLPRDSAIINRLGFNSLGHAFTHEQLAARRDRGGLVAVNLGANKDSGDRVMAYVEGVK
                     AFADVADFFVIHISSPNTPGLRYLQNQSALNVLLANVFAAREMASRRVPIVVKIAPDL
                     TLDELDGIVEVCKSCRIDGLAISNTTLSRPENLADYQVATQQGGLSGRPLFHLSTWLL
                     AHAFLRVEGAFPLIGIGGVENAATALAKIEAGADLVELYSAFIYKGIGIAADIKRGLL
                     QRLTNAGIDNLAALRGRHAQEWAAGRMSNAQLHAEV"
     gene            complement(8177..8521)
                     /locus_tag="VGB93_13010"
     CDS             complement(8177..8521)
                     /locus_tag="VGB93_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEY0147035.1"
                     /translation="MVEDIKTAPPPSGAWAPADVSDWRPQPKTPLARFLGGSPVHVFL
                     RLLVLSFLVGALLMWLDIRPYDIIDGVVRFAHRIWALGFGAVRQLGDYILAGAIIVVP
                     VWFISRLLSSRP"
     gene            8665..>10045
                     /locus_tag="VGB93_13015"
     CDS             8665..>10045
                     /locus_tag="VGB93_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147036.1"
                     /translation="MMQKHNSNAHLGRASIDAGFSFALAAFVVGLGLLAVLARIGLPD
                     IALRFCVWALIFAGFVVIATRLRTMRPAEFYAGGRNLPPAYTALAYAGLAAGLFLPFL
                     PPLLPDVSFSSLAMGFALGLAAGLCITGPYLRRTAAFSIADLVGMRFPHPLVRCVVAG
                     IAAGCAVFIALAGYDIALRAFIATTGASRSLGIFVVGFLLILLIVPGGLSGVIWLAAG
                     GTIVTLAALGLPAVLALLHQSPLGFGMTRFVQLAAMEPAPAFDPAIAVALGLGLAALT
                     PLFGPSVASDGRITALRAGPLAVFFIGVLALLSITSLSRASHALDDVLIGQDPAKVSA
                     RVPTGGTSGLTLCSTNTSDPIAIGVACTAQPGFTAGLRPRDIGASAAYLLENLPDLAH
                     LGAVLGGLASVFAMTLGIAVAASGIQSFTTSLGHDIYHPNRRRFGPVSRRLAIARALT
                     ILLVVLAGYF"
BASE COUNT         1850 a         3154 c         2980 g         2061 t
ORIGIN      
        1 cggaaatctc gtcgcagctc atgccccaga ccttttccgt ataggtcttg aagaagatcg
       61 agaagagccg ctcgccaaat tggttgcgca cccattcgtg gaaggtcttg ggatcttcca
      121 ccgggttcag cttggcatag gcataggagc cgacgcagag ggcgctggtg aaaactccga
      181 ggttgctcaa cgcctcgaaa gccttcagtg ggtaggaata gaatttatcg cgataataga
      241 tgcgcgacag gcgcggccgc tcgatgaaat cgtcgggcag gatctcattc cagagatcga
      301 ccacctcttt cgatttcgag aagaagcgat gaccgccgat atcgaagaga taattcttgt
      361 aatgcgccgt gcggctgatg ccgccgacat agtccgggtc ctgctcgatg acgaggacgg
      421 acttccccgc cttcgcgagg ctataggcgg tggtgagacc ggcgggtccg gcaccgatga
      481 tcagggcatc gagctttttc aaaaggttgc tccgcgcggg cgaatggcac gcagagaaga
      541 ccatgaagag gttaagggcc gattagagtg accacacaaa ccggaggctt gtcactcttg
      601 aatgggtgaa gcggtcgacc gccatccaaa aggaaacaaa ttcttaatgc ggcgcgacaa
      661 agcctagttt ttcggggtcg ggcgctcgcc acaggtggtc ggaagcacac cagacggcta
      721 caagaccttg gactctgcgt agctcgtagc ggtgcttgtt gcagcagtca aagaactaaa
      781 tcatctcggc gcagggatca cgtatacgtt cgttcgaata ataccggatc cctcgggcgt
      841 ttcgcacaat acgatgtcct caccaacttt gaggttgaac ggcggactgg tcaggaccac
      901 cccgctattc agcccggtcc tcacgctcgc gcccgccggt ttatcgaggt tatgggtaaa
      961 ccggccagaa gttggcaaat gttctgtaat aatcaaatat ttgaacttga cggagagttg
     1021 cgccaaagcg cgctcgatat gactatttga taaatgctgc aaaacctggc ggacaaatag
     1081 aacgtctccg cttggtattt caccgtccga taagtctagc acgcgaaaat cgacgttcag
     1141 ccctttgtac cgctgcttgt tccattcaat cagcggctcg acaatatcgc aggcgatata
     1201 attaccgcag tacggtctga gccgcgcgcc aatcgtgaag tcgctgcagc ctaaatcgac
     1261 cacactcggc ttctcggcaa atgatgacag aaattgcgac cgtgtcgata taccgtgtcg
     1321 gggtaaagcc ccctttatgc ttgtgcggcg gagaacgaaa aaaacggcct caaggcccgc
     1381 tttttattcc cgtcattcgt ctagctcggt cttaattatc caagaagctc cgcaacttcc
     1441 gtgaccggct cggatgcttg agcttgcgta gcgccttcgc ctcgatctgg cggatacgtt
     1501 cgcgcgtcac cgagaactgc tggccgactt cttcgagcgt gtggtcggtg ttcatgccga
     1561 tgccgaagcg catacgcaac acgcgttcct cgcgcggcgt cagtgacgca agaacacgcg
     1621 ttgtcgtctc gcgcagattc gactggatcg ccgcgtcgat cggcaggatg gcgttcttgt
     1681 cctcgatgaa atcgccgaga tgcgaatcct cctcgtcgcc gatcggcgtc tcaagcgaga
     1741 tcggctcctt ggcgatcttc agcaccttgc gcactttttc gagcggcatg gcgagctttt
     1801 cagcgagttc ttccggcgtc ggttcgcggc cgatctcatg cagcatctgg cgcgaagtgc
     1861 ggacgatctt gttgatcgtc tcgatcatat gcaccgggat gcggatggtg cgtgcctgat
     1921 cggcgatcga gcgtgtgatc gcctggcgaa tccaccaggt cgcataagtc gagaacttgt
     1981 agccgcggcg gtattcgaac ttatcgaccg ccttcatcag gccaatgttg ccttcctgga
     2041 tgaggtcgag gaattgcagg ccgcggttgg tgtatttctt ggcgatggag atgacgagcc
     2101 gcagattggc ctcgaccatt tcctttttgg cctgccgcgc ctcgcgctcg cccttctgca
     2161 cgacctgaac gatcttgcgg aattcctgcg tctcgacgcc ggtctccttg gagagatcgt
     2221 ggatgtccgt gcgaatttcg cggatatgat ccttgccgcg tgcggtaaaa tccttccagc
     2281 cacggccgcc gagcttcgag acccgcaaca gccatttcgg atcgagctcg gaattatggt
     2341 aattcttgag aaagtcttcg cgcgatacgc cggattcttc tgcccgacgc aacagcttcg
     2401 tctcgagccc gatgagcttg cggttgatgt cgtaaagctg ctcgaccagc gcatcgatgc
     2461 ggttctgatt aagcgacagc gatttgacgt cgacgacgat ctctttcttc agtgtcttgt
     2521 agcggcgctc ctgcgccggc gtcagcgtct cgttcttgag cttgctctcg acgttctgca
     2581 cctgaagctt gttgagcttc ttgtaggcat cggcgatgcg gtcgaaggtt tcgagcacct
     2641 tcggcttcaa ttcggattcc atcgcggaga gcgatacgga attttccata tcgtcctctt
     2701 cgtcgaagtt ttcagagatc ggcggctcgg cctcgtcgtc gccgtccccc gcgggccgca
     2761 gaccggcggg cggattgatc ggcgccgtcg ccggggcgcg ctgcgccgca agcgcctctt
     2821 gcgcaccggg cgccgtcatg tcgatcttcg gcgtgttctt gccgtccggc cctgcatagg
     2881 tcgcttcgag gtcgatgatg tcgcgcaaga gaaccttgcc ctcgttcaac tcatcgcgcc
     2941 aaataatgat ggcctggaag gtcagcggac tttcgcagag gcccgcgatc atcgcctcgc
     3001 ggccggcctc gatgcgcttg gcaatggcga tttcgccttc gcgcgacaac agctcgaccg
     3061 agcccatctc gcgcagatac atgcggaccg gatcatcagt gcggtcggcc ggctcggacg
     3121 tcttggtggc aacagctttc gactgcgtca tctcgacgag ttcgccgccc tcggcttctt
     3181 cctcttcttc ctcggcctcg gcttcgtcgg cctcttcagc ctcgatgacg ttgatgccca
     3241 tttcgttgag catcgcgaga atgtcttcaa tctgttcaga attaacttct tcggacggca
     3301 gcaccgcgtt caattcgcga taagtgacaa agccgcgctt cttggcgagc ttgatcatcc
     3361 gcttgacggc ggcatccgac atatccagca gcgggctatc ggaggtctcg acggcggcag
     3421 tgccttcggc ttcggttttc tcgttgtctt tcttcgccat cgaatcctct ttcatcgcgg
     3481 cagccaagct gccgtgccga actgcgccac gtccctggcg ccgggcggcg tcccgcccat
     3541 ttcattcctg cgggagtgca ctcccgatcg aactcaattc cctattcgcg ccgatgggcc
     3601 cgtgtctgga cacgcgaagc ccaatgacgc caattctgtc gccgtcgcat tgttgcgatg
     3661 gccgacagag gcttaaaatc tggttgcgcg ccaatcgaat tcgcttacga atttcatgcc
     3721 gaatggctgg accggacctt gtagcgtcag gcgaaaatcc cttcaagtga agaccttagg
     3781 cgcccacgct taatccatta tgccattccg ctttgccggc cggaagccat gccaaagcct
     3841 tcgatcatcg cctcggttcc cgtaagagcc gataattgct cgcgaatatc cagcaatcgg
     3901 gccaaattca cctcgctggc gtcctgcgcc aaggcgtctt gcgccgaatg caattcgcta
     3961 tttagcgccc gcatcttgtg atgcaaggcc aacgcctgtt tcaaagcctc ctcggcgtcc
     4021 gaatcagcgg cttcgggcga aacaatccag agaatcgcat gcgcgctggc atcaagacgg
     4081 cgcaaaaagg catcaaaacc ggcagtggcc agccctgccc gcaattcgtc cgcgggcagc
     4141 ggcccatgcg cccaaagatc aagcaccctt ttgcgcacgg cgagggcttc gttagcgcca
     4201 agatcaagct cagcgaaagc gtcgccataa ggatcgagaa gccccggatg attgagcaga
     4261 atcaaaaaaa tcaaagattc ccgggcggat aaatcagcca ctctcgaccg gaggaacggc
     4321 gagcgcgcga gatcggcgcc gacggagagc ggcagcgagg cataggcctg cggctcagcc
     4381 ggcgcgaacc ggccgcggcg aaattcgcgg cgctccgtct gcggctggcg attgcgggcg
     4441 gaaaaccccg ctgcattgtc aggacgccga cgctgaaaga ggccaccgag ccgctcgcgg
     4501 aattcagcct gataatgccg ccgcaacgtt tcgtcgcgga tttccccgca tagcgccaac
     4561 aggcgatgct ccagcgcagc gcggcgctcg ggcgtcgcga gcacgtcggc ttccgtctcc
     4621 cgctgccaga tcagatcgac gagcggcaac gcggcctcga tcacggcacc gattgcggcc
     4681 gatccgccgc tgcgcgccag atcgtccggg tcctggcctt cgggcagcag cgcaaagcga
     4741 agcgaccgcc ccacgccgat cagcggcagc gccagatcga tggcgcggaa ggcggctttg
     4801 cgcccggcgc gatcgccgtc aaagcagagg atcggctcct ccgccatttt ccagagcaag
     4861 tcgcattgat ccagggtgag cgcggtgccg caggcggcga ccgcctgcgg aaagcccgcc
     4921 gccgacatgg cgatgacatc gacataacct tcgacggcga tcacgctgcc tttgtcatgc
     4981 gcggctttgc gagcacgatg atgattgtag agcaccgcgc ctttgcggaa gaggaccgtc
     5041 tcaggcgaat tgagatattt tgcctgaacg tccttgctca gcgcgcggcc gccgaatgcg
     5101 ataacgcggc cgttgcggtc ggcgatcgga aacatcaccc ggtcgcggaa acggtcgtaa
     5161 ggcgcggcga tctcgtcgcc atgaacaagg aggccggcct cgatcatcgc ctcggcctga
     5221 atgcccttac tcgcgagatg atcgcgcaac gcgaatttat ccggcggcgc ataaccaatc
     5281 cggaattcgc tttgcagcgc cggggtgaga ctacgcccgg cgagatagtc gcgcgccttg
     5341 acgcccgcgc cactggccag cgtcgcaacg aaaaacgccg cagcggcctc cagaacctca
     5401 tgcaggctgg cgcgctgctt ttcctgcgcc tccgcctccg gcgtcgcgcg cggcaagggc
     5461 aacccggcgt cctgcgccag ccgctcgacc gcttcgggaa acgagagccc ttccgtctcc
     5521 atcaggaaat cgaaaatgtt gccgttctta ttcgccgaat gatcaaacca cgccatcttc
     5581 tgatcgttga cgtagaagga cggtgtgcgc tcggcgttaa aaggcgatag gccgcgccat
     5641 tcacgccccg ccttcgtcag cttgacgcga cggcgcacca cctccgacac cggaaggcgg
     5701 gctttgagat cgtccaggaa tgaggcgggg aaacgcatcg gcgccttctg atttggcctt
     5761 tcgcactata gataaggccc gcggcaggga ccaattttat tcccgctata cacagcggct
     5821 gtcgaggctt gggcatttat ggacatcaaa gcgcgttttt tcgagatgaa gcggcaaggc
     5881 cggcagatcg ccgtgctcac gggctacgat ttcccaacag ccaaggccga ggccgaggct
     5941 ggcgttgatc tcatcctcgt tggcgatagc gtcggcacca atttgctcgg ctatgccagc
     6001 gagcaggagg tgcagctggc agatatcgcg catcacgtcc gggccgtgcg ccgcggagca
     6061 cctgcgacac cgattctcgc cgacctgccc tatcgcactt acgagatgcc ggagatggcg
     6121 gcggccagcg cacgggcgct acgcgacgcc ggagcggata tggtgaaatt cgaaggcgcg
     6181 catcccgaga tcgccgcggc tattgtgagc gcaggcattc cctgctgcgg gcatctcggc
     6241 ctcgaaccgc aaacgcatct cgataagaag ctacgcgggc gctcggcggc cgaggcgacg
     6301 caattggtcc gtgacgcgat cgctctcgac aaggccggca tcgccatgct ggtgctggaa
     6361 ttgatccccg aagtcgtcgc cgcgcgtgtg acgcaggcga tcaacgcgcc gacgatcggt
     6421 atcggcgccg ggcgctacac caatggccag gttcttgtga tcaccgatct gctcggcctc
     6481 actggcgcaa gcttccgcca caaccgacgc tatgccgaac tcggcccgct gatcgcaacg
     6541 gcaatgacct cttatgtccg cgacgtccgc gaccatgcct ttccgaccga agccaacacc
     6601 ttcgccatgc cggaagaagc gctgctcgcc ttcgaaaacg gcggcggata ggctcttgcg
     6661 tcagaccaga gaacgcgcga agccttgcgc tttgggagag cgcgagcggg atggaactca
     6721 tctacaaaat tgcaccggcg gagctttggc atcaggccga gctaaggggc gttttcagcg
     6781 gctcaccggc cgatgaggcg gatggtttta ttcatctctc gacctcggcg caggtgcgcg
     6841 gtacggcggc gcggcatttc gacggccagt acaacctgct gctcgtcggc ctcgatccca
     6901 agcttctcgg ccccaaattg cgttacgagg cagcaagcgg cggggcgttg tttccccatc
     6961 tctacgcgcc cctgaagctg gatgccgtcg tctcggttgc ggctctgccg ctcggacccg
     7021 acggctcgca tcaattggac gaaaagtttc catgagccgc ccgcatctgc tgacggcagc
     7081 ggcacatgcg attgctttgc gtttgccgcc ggaaacggcg catcgcctgg cggtttgcgg
     7141 gctggggctt ctgccgatgc cggccgccgc gccggatgat ccgcgcctcg cggtgaaagc
     7201 gttcgggctt gatttcccca atcccctcgg gctcgcggca gggttcgaca aaaacggcga
     7261 ggcggtcgat gcgatcctgc ggctggggtt cggcttcgtc gaagccggca cagtcacgcc
     7321 gcggccgcag cccggcaatt cgcggcctcg gatttttcgc ctgccgcgcg acagcgccat
     7381 catcaatcgg ctcggcttca acagtctcgg acacgccttt acccatgagc agcttgcagc
     7441 ccggcgcgac cgcggcggtc tcgtcgccgt gaacctcggg gccaacaaag attccgggga
     7501 tcgcgttatg gcttatgtcg aaggcgtcaa agccttcgcc gacgtggcgg attttttcgt
     7561 catccacatt tcctcgccga acacgccggg cttgcgctat cttcaaaatc agagcgcgtt
     7621 aaatgtcctg ctggcgaatg tgttcgccgc acgcgagatg gcgtcgcggc gcgtgccgat
     7681 tgtcgtcaag atcgcgccgg acctgacgct cgacgaactc gacggaatcg tcgaggtttg
     7741 caaaagctgc cgcatcgatg gtctggcgat ctcgaacacg actttgtcgc ggccggaaaa
     7801 tctcgccgat tatcaggttg ccactcagca aggcggcctt tccggccggc cgctgtttca
     7861 cctttcgacc tggctgctcg cgcacgcatt tttgcgtgtc gagggtgcat tcccactgat
     7921 cggaatcggc ggcgtcgaga atgccgcaac cgcgctcgcc aagatcgaag cgggcgccga
     7981 tctcgtggag ctctattcgg ctttcatcta caaaggcatc ggcatcgccg cggacatcaa
     8041 gcgcggattg ctgcagcggc ttacaaacgc gggcatcgac aatctcgccg cgcttcgcgg
     8101 ccggcatgca caggaatggg cagcaggccg aatgtccaat gcccaattgc acgccgaagt
     8161 ctgatcagga gtccggttag ggccgcgacg acaaaagccg cgagataaac cagaccggca
     8221 ccacgatgat tgcgcctgcc agaatataat cgccaagctg gcggaccgcg ccgaagccga
     8281 gcgcccagat tcgatgcgca aagcgcacga cgccatcgat gatgtcgtaa ggccggatgt
     8341 cgagccacat cagcagcgcg ccaacgagga acgacagcac aagcagccgc agaaacacat
     8401 gcaccggcga gccaccgaga aagcgagcca gcggcgtctt gggctgcggt ctccagtccg
     8461 aaacatcagc cggcgcccaa gcgccggaag ggggcggtgc tgtcttgatg tcctcgacca
     8521 tgatggcctc tctggcaaaa tgcgaggcat atgtaggcgc gccccgacca agaccaagtg
     8581 cgccgcctca cagtgccggt ggctatgtcc caacgattac gatggcttta tgatgggctt
     8641 tgcccgcttg gtcctaggtt cccaatgatg caaaagcata attccaacgc ccatctcggg
     8701 cgcgcttcga ttgatgctgg cttcagcttc gctctcgcgg ccttcgtcgt tgggctcggt
     8761 ttgttggcag tgctggcgcg catcggctta cccgatattg ccctgcgctt ttgtgtttgg
     8821 gccctgattt tcgccggctt cgtcgtcatc gcgacacggt tgcgcacgat gcgacctgcg
     8881 gaattttacg ctggcgggcg caatctgccg cccgcctaca cagcgcttgc ctatgccggc
     8941 ctcgcggccg gattgtttct gccgtttttg ccgcccctcc tgccggatgt cagcttttct
     9001 tcgctcgcca tgggcttcgc gctggggctg gctgccggac tctgcatcac cggaccttat
     9061 ctgcgccgca ccgccgcttt ctcgatcgcc gatctcgtcg ggatgcgttt cccgcatccg
     9121 ctggtgcgtt gcgtcgtcgc cggcattgcc gccggctgcg ccgttttcat tgcgctggcg
     9181 ggttatgaca tcgcgctgcg cgcctttatc gcaacgacag gggcgagccg cagcctcggc
     9241 attttcgttg tcggtttcct gctcattctt ttgatcgtgc ccgggggcct ttccggcgtc
     9301 atctggcttg cggccggcgg cacgatcgtg actctggccg ccctcggctt gcccgcggta
     9361 ctcgcactgc tgcatcaatc gccgcttggg ttcggcatga cgcgcttcgt gcaactcgcg
     9421 gcgatggagc ctgcgcccgc gttcgatccc gcgattgccg tcgcgttggg gctcgggctt
     9481 gcagccctga cgccgctgtt tgggccctcg gtcgccagcg acggacgcat tacagcacta
     9541 cgggccgggc cgctggcagt gtttttcatc ggcgtgctgg cgcttctttc aatcaccagt
     9601 ctatcccgcg catcccatgc gctcgatgac gtcctcatcg ggcaagaccc ggctaaggtc
     9661 tccgcccggg tgccgacagg cggtacaagc ggactgacgc tctgcagcac aaataccagc
     9721 gatccgatcg ccatcggtgt cgcctgcacc gcgcagcccg gcttcaccgc aggtttacgg
     9781 ccgcgagaca tcggcgcgag cgccgcctat cttctcgaaa acctgcctga tctggcgcac
     9841 ctcggcgcag ttctcggcgg cctggcgtcc gtctttgcga tgacgctcgg catcgccgtt
     9901 gcagcatccg gcatccagtc cttcacgacg agtctcggcc acgacatcta tcacccaaat
     9961 cgccgccggt ttggccccgt gagccgccgt ctggcgatcg cgcgggctct gacaatcctg
    10021 cttgtcgttc ttgcgggata tttct
//
LOCUS       DASWOG010000632         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_107761,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000632 DASWOG010000000
VERSION     DASWOG010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107761"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VGB93_13020"
     CDS             <1..1243
                     /locus_tag="VGB93_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0147037.1"
                     /translation="DLRVAAASIARAEAGADIAKAAQFPTTTLEANPTFTRLSPQEQL
                     LPNVDINPQGLYSLGGGLSYQVDLFGQIRRAIESAKADVVAAHEAYDVTRITIVAETA
                     KAYADVCSAGRQIAIAERSVALQTRSTQLTRELFQTGRGISLDVTRAKALEDRVRASI
                     PPLDANQKVALYRLTVLTGRPPEEFPVSVVHCADEPRLGQPVPIGDGAALVARRPDIR
                     RAEAIFHATTAQVGVAIADLYPKVTFGLSAASVGPNVAFLQYSTLKYSAGPLITWDFP
                     NRLVAEAHIRGAKADVEVAFAQFDGAVLAALREVESALVVYARDLDQRKLLAASRDQA
                     AIAAGDAETLFKAGSTNYLTVLDANLALITADQSLAMLDGKIVDDQITLFLALGGGWE
                     SPRLLPHKPIVTPAPQTANRL"
     gene            1360..1599
                     /locus_tag="VGB93_13025"
     CDS             1360..1599
                     /locus_tag="VGB93_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147038.1"
                     /translation="MRVRATIVAIAAGILVATGANAVNIQETVEMITHSSVTMKDGTV
                     MPMEIVRMHGKLYVMMSVDDLPDYLHQQVLKVMPQ"
     gene            1854..2342
                     /locus_tag="VGB93_13030"
     CDS             1854..2342
                     /locus_tag="VGB93_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147039.1"
                     /translation="MRLNPLRPKFFAALFAAALMTSTGDSSFAGPFSSLAGNWAGNGT
                     LTMSGTRERIRCRAIYFVSSGGESLQQILRCASDNFIIDVHSNVIEIDGRLSGTWQET
                     SSGVSGSLSGIARGGTIRGLISGLGVTASLSLITRSRSQYASIGLSGATTATVVVNFH
                     RI"
     gene            2428..2640
                     /locus_tag="VGB93_13035"
     CDS             2428..2640
                     /locus_tag="VGB93_13035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0147040.1"
                     /translation="MAFCVPAKADPDALHAFLESLWPRASAQGISRATFVRALDDLSE
                     DPSVPIAVAKQPEFERLLSAYFKEAV"
BASE COUNT          546 a          856 c          839 g          610 t
ORIGIN      
        1 cgacctgcgt gtcgcggccg cgtcaattgc acgagcagag gcgggcgccg acatcgccaa
       61 ggccgcgcag tttccgacga cgacgctcga agccaacccg accttcacgc gcctctcgcc
      121 ccaggaacaa ttgcttccga acgtcgatat caacccgcag ggcctctaca gccttggcgg
      181 cggcctttcc tatcaagtcg atctcttcgg ccagatccgc cgtgcgatcg aatcggccaa
      241 agctgacgtc gtcgccgcgc atgaggccta tgacgtgacc cggatcacta tcgtcgctga
      301 gacggccaag gcctatgccg atgtctgctc cgccggcagg cagatcgcga tcgccgagcg
      361 gtcggtcgct ttacagacgc gaagcacgca actcacgcgt gaactttttc aaaccgggcg
      421 cggcatttcc cttgacgtga cgcgggcgaa ggctctagaa gatcgcgttc gcgcaagcat
      481 tccgccactc gatgccaatc aaaaagtcgc gctctatcgt ttgacggtcc tgaccggccg
      541 gccgccggag gagtttccgg tctcagtcgt gcattgtgcc gacgaaccac ggctcggcca
      601 accagttccg atcggtgacg gtgcggcgct cgtcgcgcgg cgtcctgata tccgccgcgc
      661 ggaagcgatc tttcatgcga cgacggcaca ggtcggtgtt gcgatcgcgg atctctatcc
      721 gaaggttacg ttcgggcttt ccgccgcgtc ggtcggcccg aacgtggcgt tccttcaata
      781 tagcacgcta aaatattccg ccggtccatt gatcacctgg gactttccca atcggcttgt
      841 ggctgaagcg cacatccgcg gcgcaaaagc cgatgtcgaa gtcgctttcg cgcaattcga
      901 tggcgcggtg cttgcggctc tgcgcgaagt tgaaagcgcg cttgtcgtct atgcgcgcga
      961 tctcgaccag cgcaaacttc tcgccgcatc gcgcgatcag gcggcgatcg ccgccggcga
     1021 tgctgagacg ctcttcaagg ccggaagtac aaattacctg accgtgttgg atgccaacct
     1081 cgcgttaatt accgcggatc agtcgcttgc catgctggac ggtaaaattg tcgatgatca
     1141 gatcacgctt tttctagcgc tgggtggcgg ctgggaatcg ccgcgcttgt tgcctcataa
     1201 gccgatcgtg acgccggcac cgcaaactgc caaccgttta taatagtcgc cagcttgcgc
     1261 cgcgccacgg cgaacgtttc acgccagcgg gattgcgcgc cctatcgaag aaagatagtt
     1321 tcccatcgtc ccaatttggg gaacaaaagg agtgcgaata tgcgagttag agcaactatc
     1381 gtcgccatcg ccgccggaat tctggtcgcc acaggcgcca acgccgtcaa cattcaagaa
     1441 actgtcgaaa tgatcacgca ctcgtccgtc acgatgaagg acggcacggt gatgccgatg
     1501 gagatcgtta ggatgcacgg gaaactgtat gtcatgatgt ccgtggacga tcttcctgac
     1561 tatctgcacc agcaggtcct gaaggtgatg ccgcaataaa ttttcccttg attttgcggc
     1621 cgcgccgagc cgcgatcgga atatatcttt aaagggccgg cctcgcggcg catacgctgc
     1681 gaggcttttt gctgggctga aaacaaaaaa gcgcaccgac gatttcggta gattttcgat
     1741 gcctctggat agcatgaatg gcatgaatgc aaaccgctcg cgcctccggt ggccgggaca
     1801 tacgccggcg cagcggcttt gatgactcca caccgttatg agacggaggc cgaatgcgtt
     1861 tgaacccgct gagaccgaaa tttttcgcgg ccttgttcgc tgctgcgttg atgacttcga
     1921 caggcgattc ttcttttgcc ggtccgttct cgtcgttggc tggaaattgg gcaggcaatg
     1981 gcacgctcac catgagcggc actcgcgaaa gaattcgctg ccgcgcgatc tatttcgttt
     2041 cgtctggcgg cgagagcctg caacaaatcc tgcgctgcgc gagcgacaat tttatcatcg
     2101 atgtgcatag caatgtcatt gaaatcgatg gaaggctctc gggcacgtgg caggagacgt
     2161 cgagcggcgt gagcggctct ttgagcggga tcgcgcgcgg gggcaccatc cgaggcttga
     2221 tctcggggct cggcgttacg gcgtcgcttt cattgatcac gcgcagccgt tcgcaatatg
     2281 cttcaatcgg ccttagcggt gccacgacag ccaccgtcgt cgttaatttc catcggatat
     2341 aggaacggct tcgcccggat cgacggcaac gtcatgcaaa gacttagctt gaccgcacgc
     2401 tgctattttt ttgcgatttg cttatcgttg gctttctgcg tcccggcgaa ggccgatcca
     2461 gacgccttgc atgcttttct cgaaagcctt tggccgcgtg cctcagcgca gggcatcagc
     2521 cgtgcgacgt ttgtccgggc gttggacgat ttgagcgaag acccgtcggt gccgattgct
     2581 gttgccaagc agccggagtt cgagcggctg ctgtctgcct atttcaagga ggccgtatag
     2641 ccgacgcgca ttgctcgcgg ccgggcgctg gcagagacct atcatcgcca gcttgacacc
     2701 gtcgtgcgcc gctacggcgt gccgcgcggc attctgctcg ctgtctgggg catggaaagc
     2761 gattttgggc gcgaccgcgg cgacaaggac gtcatccgct cgctcgcaac gctcgccttt
     2821 caccgcagag ataacgatct cttcgccaac g
//
LOCUS       DASWOG010000633         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_309882,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000633 DASWOG010000000
VERSION     DASWOG010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309882"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1011)
                     /locus_tag="VGB93_13040"
     CDS             complement(112..1011)
                     /locus_tag="VGB93_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY0147041.1"
                     /translation="MKGATGIFVTLLAIAAIIVIVASIFTVGQTEQALVLRFGDPVAG
                     RGLVTEPGLHFKIPFIENVVLLDKRILDVETPTQEVLASDNTRVEVDSFLKYRIVDPL
                     KFYQTVGDIDRANDQLGYILNSAVRRVLGDADLRQIVSADREALMANIRQQVNQQADK
                     LGVSAVDVRIRRTDLPQQISEKVFSRMQSEWAREAALFRAQGAEESQEIRAKADRDAV
                     VLRADAQRDADQTRGAGDAQRARIFATAFGKDPDFFAFYRSMQAYTTGLKASNTRLVL
                     SPKSAFFQYFLPPAGANTVPPAH"
     gene            complement(1008..>1680)
                     /gene="hflK"
                     /locus_tag="VGB93_13045"
     CDS             complement(1008..>1680)
                     /gene="hflK"
                     /locus_tag="VGB93_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0147042.1"
                     /translation="TDDQNIADVKFVVIWQIDPSHPEDYAFNIDDPDDTVKAVAESAM
                     RAVIGRSLIQNILTADRKVIEPAVQDLAQKIFDKYKAGVLILQVQLQSVDPPQEVITA
                     FRDVTAAQQDRDRMRNEAQTYANNVVPAAQGQAAAIRQQAEGYRLQTIDEATGQASRF
                     SQVEAQYKIAPALTRERIYLETMEQVLGGSDKVIVDSGNGQSVLPYLPLPLLTPNASA
                     DGAPK"
BASE COUNT          332 a          496 c          499 g          353 t
ORIGIN      
        1 cccaataaag cagacgcgaa ttgccggcat gaagccattc atctctcctc aagcgcgcaa
       61 aattagcgga tcagctaaac gaattcacac cgatttttgc gcacgatcgc gttaatgcgc
      121 gggcggcacc gtattggctc cggctggcgg caaaaaatat tggaagaagg ccgatttcgg
      181 gctgagcacc agacgggtgt tcgacgcttt caagcccgtc gtataggcct gcatcgaacg
      241 gtagaaggcg aaaaagtctg ggtccttgcc gaaagccgtt gcaaaaatgc gcgcgcgttg
      301 ggcatcgccg gcgccgcgcg tctggtcagc atcgcgttgc gcgtcggcac gcaaaacaac
      361 ggcatcgcgg tccgccttgg cgcggatctc ctgcgattcc tccgcccctt gggcgcggaa
      421 gagcgctgcc tcgcgcgccc attcgctctg catccggctg aagacctttt ccgatatctg
      481 ctgcggcaga tcggtgcgcc ggatacggac atcgacagcg ctgacgccga gtttatctgc
      541 ctgctgattc acttgttggc ggatattggc catcaaggct tcgcgatcgg cgctgacgat
      601 ctgtctaagg tcggcgtcgc caagaacacg gcgcacggcg gaattgagaa tatagccaag
      661 ctggtcgttg gcgcggtcga tgtcgcccac cgtttgatag aatttgagcg gatcgacgat
      721 cctgtatttc aggaaggaat cgacctcgac ccgcgtattg tcggaggcga gcacttcctg
      781 cgtcggcgtc tcgacatcga ggatgcgctt gtcgagcaga acgacattct cgatgaacgg
      841 aattttgaag tgcaggcccg gttcggtgac gaggccgcgc cccgcgaccg gatcgccgaa
      901 acgcagcaca agcgcctgtt cggtctgacc gacggtgaag atcgacgcca caatgacgat
      961 gatggcggca atcgccagaa gcgtcacgaa aataccggtc gcgcccttca tttcggcgct
     1021 ccatcggcgg atgcgttcgg cgtcaaaagt ggcaatggca aatagggcag tacgctctgt
     1081 ccattccccg aatcaacaat gaccttgtcg ctaccgccga gaacctgttc catcgtctcg
     1141 agatagatgc gctcgcgcgt caacgccggc gcgattttgt attgtgcctc aacttgcgag
     1201 aagcgcgacg cctggccggt cgcctcgtcg atcgtctgca agcgatagcc ctcggcctgc
     1261 tggcgaatgg ccgctgcctg accctgtgcc gctggcacca cattattggc gtaggtctgg
     1321 gcctcgttgc gcatccggtc gcgatcctgc tgagcggcag tcacatcgcg gaaggcggtg
     1381 atcacttcct gcggcggatc gaccgactgc aattgcacct gaaggatgag gacgccggct
     1441 ttgtatttgt cgaaaatctt ctgcgccaaa tcctgaaccg ccggttcgat cactttgcga
     1501 tcggcggtca ggatattctg gatcaggctg cggccgatca ccgcgcgcat cgcgctctct
     1561 gccaccgcct tcaccgtgtc gtcgggatcg tcgatgttga aggcgtaatc ttccggatgc
     1621 gatggatcga tctgccagat cacgacaaat ttcacatccg cgatgttttg atcgtcggtg
//
LOCUS       DASWOG010000634         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_216707,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000634 DASWOG010000000
VERSION     DASWOG010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216707"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VGB93_13050"
     CDS             complement(<1..721)
                     /locus_tag="VGB93_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY0147043.1"
                     /translation="MPGSARKGLVVPLLFAFAATALLVGLGVWQLHRLTWKEGLIARI
                     AARGAAAPQKLPNPASWPRLKPEDYEYQHVALRGRFDNAKEVLVFDTSGPGDVGPGYL
                     VLTPLILASGDQVIVNRGFVPAALAAKSARPKTEIEGDVTVTGLMRPPQSRNFFTPAD
                     DPAKGLYFTRDPLAIAAHSGLAHAAPFVVDQDKTAIAGGWPAGSLNGVNIPNNHFEYA
                     LTWFGIAGGLWIVVGGYLLRTW"
     gene            complement(729..1121)
                     /locus_tag="VGB93_13055"
     CDS             complement(729..1121)
                     /locus_tag="VGB93_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY0147044.1"
                     /translation="MSHLENSGPESRSVASTVWAGVAGHCPRCGRGKMFSKLLTVAPG
                     CDVCGLSYGFADAGDGPAVFVMLFAGFLIVGLALLIEVAYEPPYWVYFVIFLPLTIVV
                     CLGLLRPLKGIMLALQYRNKAELGRLES"
     gene            complement(1170..2033)
                     /locus_tag="VGB93_13060"
     CDS             complement(1170..2033)
                     /locus_tag="VGB93_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0147045.1"
                     /translation="MSDAHAKPAHPYHLVDPSPWPIIGAFGAFLMAVGAVLWMKSIPI
                     GAVKPGGYVFFAGVICVLFVMANWWLDVIKEAQTPGVHTPVVSLGLRYGMILFIASEV
                     MFFVAWFWAFFDAALFPNEAIEYARQTFTGGHWPPKGIESIDPWHLPLLNTLILLTSG
                     TTVTWAHHALLNNDRKGLVNGLILTVLLGISFTCVQAYEYAHAPFAFKGSIYGSTFFM
                     ATGFHGFHVIIGTIFLIICLLRALAGQFTPQRHLGFEFAAWYWHFVDVVWLFLFSCIY
                     VWGSWGGPFEH"
     gene            complement(2115..>2363)
                     /locus_tag="VGB93_13065"
     CDS             complement(2115..>2363)
                     /locus_tag="VGB93_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0147046.1"
                     /translation="VYNVSPGQTGAYFDKLACFCFTEQHFGPHQTAEMPVVFFLDPAL
                     EKDDTMSGINEVTLSYTFYPAHDGQPLAQATGTVPPRL"
BASE COUNT          542 a          704 c          733 g          384 t
ORIGIN      
        1 gccatgtccg caaaagataa ccgcccacga caatccacag gccacccgcg atgccgaacc
       61 aggtcaaggc gtattcgaag tggttgtttg gaatattgac gccgttcagg ctcccggctg
      121 gccagccgcc ggcgatcgcg gtcttgtctt gatcaacaac gaacggcgcc gcatgagcga
      181 ggccggaatg cgccgcgatc gccaaaggat cgcgcgtaaa ataaagaccc ttcgcaggat
      241 cgtcggcggg cgtgaaaaaa ttgcgcgact gcggcggccg catgagcccg gtgacggtca
      301 cgtcgccttc aatctcggtt ttggggcgcg cacttttcgc cgccagcgcc gccggtacaa
      361 aaccgcggtt gacgatcact tgatcgccag aggcgaggat caacggtgtt aggacgaggt
      421 agccgggccc cacgtcaccc gggccactgg tatcgaacac cagcacttcc ttggcattgt
      481 cgaaacggcc gcgaagcgcg acgtgctgat attcgtaatc ttcgggcttg agacgcggcc
      541 agctcgcggg attaggcagc ttttgtggcg ctgccgcgcc gcgcgccgcg atccgcgcaa
      601 tcagcccttc cttccaggtg agacgatgca attgccagac gccgaggccg acgagaagcg
      661 ccgtcgcggc gaaagcaaag agcaaaggga cgacgagccc cttgcgtgct gacccgggca
      721 tcgcgcgtct agctttcgag ccggccgagt tcggccttat tgcgatattg cagcgccagc
      781 ataatgcctt tcagcggccg caaaagcccg aggcaaacga caatcgtcaa cggcaggaag
      841 atgacgaaat agacccaata cggcggctcg taggcgacct cgatcaacag agcgaggccg
      901 acaatcagaa agccggcgaa gagcatgacg aacaccgctg ggccgtcgcc cgcatcggca
      961 aagccataac tcaggccgca gacatcgcag cccggcgcaa ccgtcagcaa tttggaaaac
     1021 atcttgccgc gcccgcagcg gggacaatgg ccggcaacac cggcccaaac cgtcgaggcg
     1081 acagatcgcg attcggggcc gctattttcg agatgactca tggcggcctt gtgcgcccgg
     1141 cagacgccgg gcgcaagctt ttcgcaaact taatgttcga acggaccgcc ccaggatccc
     1201 cacacataga tgcaggagaa gaggaacagc cacacgacgt cgacgaaatg ccagtaccag
     1261 gcggcaaatt cgaagccgag atgacgctgc ggcgtgaact ggccggcaag cgcacgcagg
     1321 aggcagatga tcaggaagat cgtgccgatg atgacgtgga agccgtggaa gcccgtcgcc
     1381 ataaagaagg tcgagccgta gatcgaaccc ttaaaagcaa acggcgcgtg ggcatattcg
     1441 taggcctgca cgcaggtgaa ggaaatgccg agcagaaccg tcaggatgag gccgttgacg
     1501 aggcccttgc gatcgttgtt gagcaacgcg tgatgcgccc acgtcactgt cgtgccggag
     1561 gtcagcagga tcagcgtatt gagcagcggc agatgccagg ggtcgatcga ttcgatgccc
     1621 ttcggtggcc aatgaccgcc agtgaaagtc tggcgcgcat attcgatcgc ctcgttcggg
     1681 aacagggccg catcgaagaa cgcccagaac cacgcgacaa agaacatgac ctcggaggcg
     1741 atgaaaagta tcatgccgta gcgcaggcca agcgagacga caggcgtatg cacgccgggc
     1801 gtttgcgctt ccttgatgac gtcgagccac cagttcgcca tgacgaacag cacgcagatc
     1861 acgccggcga agaaaacata gccgccgggc ttcacggcgc cgatcgggat cgacttcatc
     1921 cataagacgg cgccgaccgc catgagaaag gcgccgaatg cgccaatgat tggccatggg
     1981 ctgggatcga ctaagtgata tggatgggcc ggtttggcat gcgcgtcaga catttcgttc
     2041 cctctcgtct ctccggggtt tgacacccct gcggccccct tgtcccgtgc cgcgtttcct
     2101 cgacctatgc gtcatcagag ccgcggcggc acggtgccag tggcctgggc caaaggctgt
     2161 ccgtcatgcg cggggtagaa cgtgtaagac agcgtcacct cattaatgcc gctcatcgtg
     2221 tcatcctttt ccaacgcggg gtcgagaaag aagacgacag gcatctcagc cgtttgatgc
     2281 ggaccgaaat gctgctcggt gaaacagaag caggcgagct tgtcgaaata ggcgccggtc
     2341 tggccgggcg aaacattata gac
//
LOCUS       DASWOG010000635        10645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_236337,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000635 DASWOG010000000
VERSION     DASWOG010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236337"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..357
                     /locus_tag="VGB93_13070"
     CDS             52..357
                     /locus_tag="VGB93_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY0147047.1"
                     /translation="MSKLIVLYKKPADPAHFDKHFREVHVPLVKKMPGLRATSFGPAS
                     TLDGGEGAFFWIFIGTFDSLKAIHEALASPEGQKVVADIPNYSADAPTILYLESTEG"
     gene            complement(377..1663)
                     /locus_tag="VGB93_13075"
     CDS             complement(377..1663)
                     /locus_tag="VGB93_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0147048.1"
                     /translation="MKVRPKFRQIVTDPVLALMLALGFSSGLPFLLILGTQAARLAEA
                     KVPIEEIGLISWVGLCYSLKFFWAPIIDEVDVPYLARLLGRRRAWLLVAQVGIACGLV
                     ALAFADPARSFKGLIVCAVFTAFAAATQDIVIDGWRIDSAPPERQGVMAATYNFGYRL
                     GLLAAGAGALYIADYSGWRAAYLIMATLMLVGVIAGILSPRLPERARDKTQSFAMNFI
                     APIVDLARRFGPLLAVILLLVALYRLPDFLSGVMANPLYIGLGFSKSQIATMSKVYGI
                     WIGIAGAFAGGFTITRIGLMPALLVGGIAASASHLCLALLAATGKSLPLLALSVSVEN
                     FAGNFAGAALIAYMSSLTSPAYAASQYALLSSLYALLGKFFGGLSGFAVHQIGFAKFF
                     ASTSLIGVPVAALCLFLWARQGRTPQPVPQSEEPAA"
     gene            complement(1709..3838)
                     /locus_tag="VGB93_13080"
     CDS             complement(1709..3838)
                     /locus_tag="VGB93_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HEY0147049.1"
                     /translation="MKDAYEPDVMVDDASHDMSLGVLGAALYARKYWVLIPTLLAFLL
                     ALAYVTIAKPRYTADAQILLENQESYLTRAQRTEGMETAPAIDETWIGSQVSLITSRD
                     VAQEVIEKLHLVGNPELDPVSKGLGVIQRTLVLFGLAHDPTRVSAEDRTIATFSERLT
                     AYSPIKTQVVMVDFWAHDPVFAAKVANEVAAVYLERQTDAKRDLAKDVSDALGGQINT
                     LNEKLARASDEVERYRAASGLLAGANNMTITAQQLADLNTDLTRARTDEANSQAKAAL
                     IRQLLRKGRVSEIPDVANNDLIRRISEQRVTANAQLALEAGTLGPRHPRILELKAEVE
                     DLNAQLRLAGDNIAISLENDSKIAASRVANIQTALDQQKQAVETANTNSVQLRALEQV
                     EAGYREQLQSATTKYQEAVSRQSGATPADARIIAAATAPDQPSFPKKPQVLGLATLAA
                     FVLSLGIVVGRELLADRPLSDATGDDVLVMPVRLAEEKPRPAVSTIERLKRFASARVP
                     ESDDEELVAAPAPDESDEKAAAKSTTPQGVNIVATRLNKSKEATDALISFARTLARDG
                     RPIIVDLDSRDGQLAKLIGPEAQDGKMMGLTDLLDGDASFADVIHRDAASRLHFVPFG
                     SKDTFNPDDLDIVLDALARTYDFVILAAPPFPSNPMTKALAPYADFVVLATAGERDDA
                     ATQTARAELGAAGAPEILLVGGAKQAA"
     gene            4089..4604
                     /locus_tag="VGB93_13085"
     CDS             4089..4604
                     /locus_tag="VGB93_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0147050.1"
                     /translation="MLGACAATTYSAALDTDPNAPYTLGSGDRVRVIVFGQDSLSNSY
                     AIDGAGHISMPLIGSVGATGETTTSLARKIEARLSDGFIRDPHVSVEVEAFRPFFVLG
                     EVTTPGQYPYVNGMTAETAVAIAGGFTPRAYKDTVDLTRTIDGHPLTASVPILHMIKP
                     GDTIVVRERYF"
     gene            4620..5819
                     /locus_tag="VGB93_13090"
     CDS             4620..5819
                     /locus_tag="VGB93_13090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0147051.1"
                     /translation="MPMQQIVPERKLRILHVFRAPLGGLFRHVIDLAREQVARGHAVG
                     VIADSLTGGSRANEVLGDLAPSLELGLCRIPMHRPAHILDAVAAARVARHADRLDVDV
                     VHGHGSKGGAYTRLPALLPTKRKAIRAYTPHGGSFNYRGGPLVERGYMMAERLLTAGT
                     DVFLFESAFVAQQFRRRVGPTTKLTRIVYNGLSPTEFLPTEPRADAAEFVYIGELRAA
                     KGIDTLIDALALLSKTGIQPRLVLVGTGPDERELITRAETRGVSTQVTFAGLVPARLA
                     FPLGRILVVPSRAESLPYIVLEATAAQIPIVATDVGGINEIFGPFRDRLIAPDDPQIL
                     ADTLQKMLAHSPAESRADSQALASFVKTKFKISDMADSVLAGYAEAIANRDHRGATAA
                     SFALPSS"
     gene            5832..7367
                     /locus_tag="VGB93_13095"
     CDS             5832..7367
                     /locus_tag="VGB93_13095"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY0147052.1"
                     /translation="MVKFSLSDLQKTEPVDKAAPAADMSSEALAAAARAMQSSSSMPG
                     LSRVVLEGLVRCVEFLLIAAAGFAIHFFYIARTQGYAQPYLIGVPCVAAAAVLALQSA
                     ALYKISALRRFPADSFRLAGALTFVFAATLVLVFFLKLDEVFSRVFMLAWYGGSLVAL
                     CGERAALALLVDRLIQTGRLARRTVLVGGGTAGEALLNQLAAQPQPDLQILGIFDDRT
                     DERSPAVVAGFPKLGTVDDLLTFSRRTRIDLIIFTLPITAEQRILQMLRKLWVLPIDI
                     RLAAHMNKLHFRPHSYSYIGSVPLIDIFDKPIAGWDVIVKYAFDKIVGALCLILLAPV
                     MAIVALAVKLDSKGPALFRQKRYGFNNEIVEVYKFRSMRTDMLDFAATKLVTKDDPRV
                     TRVGRFIRKTSLDELPQLFNVVFKGNLSLVGPRPHALHAKAAERQYDEVVDGYFARHR
                     VSPGITGWAQIHGWRGETDTPEKIQGRVECDLYYIENWSILLDIYILLATPFALLRTK
                     NAY"
     gene            complement(7383..8687)
                     /locus_tag="VGB93_13100"
     CDS             complement(7383..8687)
                     /locus_tag="VGB93_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147053.1"
                     /translation="MVRGPSQNEPDAPISIIQLPNGTFRGFAANAETIAIDGKTPLDL
                     GGTSKVVLKPGPKNSSSECGQWLTTLMPVRDLLYGMIHDEQHCNYNAGETQKSMSIAI
                     SHDEGLTWNVLGQIITGDESDVENGPSGVGDCTAVDGHDGYWYAYCLRLRDWKNIAAR
                     APHDAPTPGKWLEWDGVGWHEPGLGGMAAPLIPEAGMSAAYWTSIGAVMLLNAADTSL
                     QLSVSKNKMDFNTLPEPLIPYDAGTWKRPAPTELYAYPAMIADKGLNNISHKFLLAYM
                     YIPPGQDFTQRYLVIQEGRIHLSPVPRSPQVRTALARWITPQHMTWTTRGPAISAGRS
                     YTYDRGLGYLMTAAPNDADSVELDECFSPQTCVGFLAEAGDCPAEGSARRRTAGYVFR
                     HHRPGTMALIGCVKEDGARFVSNRPDCENVGTRERFLGFALQ"
     gene            complement(8827..10113)
                     /locus_tag="VGB93_13105"
     CDS             complement(8827..10113)
                     /locus_tag="VGB93_13105"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0147054.1"
                     /translation="MAISQSGRALIVEGDPPTALSRRRAAWTRWRMNIFLLAVIAINY
                     TWARPSPVDVLFFGALLMTVFCNQLINTRTFFFICLILVWLSSVYISSMSLTADPRVV
                     FEMFALTSVAFIGITSCLVATAWTERELRIFVDVYILSNVIAAVIGILGFVAHNPDLT
                     WADRPRAFLDDPDMFGVFLVPGILGSLFMIAEKRRRILFSAALLLLSVPVFLSFSRAA
                     IVSAALWGGIYLGILNRHQLLRATLSSLAFLVPLVVVVLLFAVLNDTVSHMLSGRFTV
                     AEEYDLGHFGRYNRYLLAIPMILDHPFGLGLLQIENYFPEPIHNVWIACFLYFGWIGG
                     LAWTLLLILSGMQAWNTWKQSRNGLCLMIFFGWLSIISCSMLHEGERWRFMWLLTGVL
                     WGLNPRNFAAIPGPAHESRRVVATTKRSGDARPVVS"
     gene            10295..>10645
                     /locus_tag="VGB93_13110"
     CDS             10295..>10645
                     /locus_tag="VGB93_13110"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY0147055.1"
                     /translation="MMYKWANIVIGLAALSGAAGIMESAASAHTIADPLLKTSANMLI
                     VNAASVIAISGYASSRLQHWALFGAATLLAGGLLFCGELSTHVFLGQRSFALAAPIGG
                     TLMIAGWVIAAASAF"
BASE COUNT         2258 a         3203 c         3066 g         2118 t
ORIGIN      
        1 gtagaaggct ccagtaatgg cgcgccagcg ccggcttcga ggagtatcca cgtgagcaag
       61 ctcatcgtcc tttataagaa acccgccgat cctgctcatt tcgataagca ttttcgcgag
      121 gtccatgtgc cgctcgtgaa aaaaatgccc gggctgcgcg cgacgtcctt cggtccagcg
      181 agtacgctcg acggtggcga aggcgctttt ttctggatct tcatcggcac gttcgacagc
      241 ctgaaggcca tccatgaggc tctggcttcg ccggaggggc aaaaggtcgt cgccgacatt
      301 ccaaattatt cagccgacgc accgacgatt ctttacctgg agtccaccga gggttgaggc
      361 cggcctctcc gtctcgttac gcagccggtt cctctgattg aggaaccggc tgcggagtgc
      421 gcccttgtct cgcccataaa aacaaacata gcgccgcgac gggcacgcca atgagcgacg
      481 tgctggcaaa gaatttcgcg aagccgatct ggtgaaccgc gaaacccgag agtccgccga
      541 aaaatttgcc aagcagcgca tagagcgaac tcagcagcgc gtattggctc gcggcatagg
      601 ctggcgatgt cagcgacgac atataggcga tcagcgccgc gcccgcgaaa tttccggcaa
      661 aattctcgac gctgacggat agcgcgagca gtgggaggct tttgcccgtc gccgcgagca
      721 gcgcgaggca gagatgcgag gcagaagctg caatcccacc gacgagcagc gccggcatca
      781 gcccgatgcg cgtgatcgtg aacccgccgg caaaggcccc ggcaataccg atccaaatgc
      841 cgtagacctt cgacatggtc gcgatctggc tcttggaaaa gccaaggccg atataaagcg
      901 gattggccat cacgccgctc aggaaatccg gcagccggta gagcgcgacc agcagcagga
      961 tcaccgcgag tagcgggccg aaacggcgcg ccagatcgac gatcggcgcg atgaaattca
     1021 tcgcgaacga ttgcgtctta tcgcgcgcac gctccggcag gcgtggcgac agaatacctg
     1081 cgatgactcc gacaagcatg agtgtcgcca tgatgagata agccgcgcgc catcccgaat
     1141 aatccgcgat ataaagcgcc cccgccccag ccgcgagaag gccgagccgg tagccaaaat
     1201 tataggtcgc agccatcacg ccctggcgct cgggtggcgc agaatcgatg cgccagccgt
     1261 cgatgacgat atcctgcgtc gcggcggcaa aggcggtgaa gacggcacaa acgataagac
     1321 ccttgaacga acgggccgga tcggcaaagg cgagcgcgac gaggccgcag gcaatgccga
     1381 cctgcgcgac cagcagccag gcccgccgcc gcccgaggag cctggcgaga tatggcacat
     1441 cgacttcatc gatgatcggc gcccagaaaa attttagcga atagcaaagg ccgacccagc
     1501 tgatcagtcc gatctcttcg atcggcacct tggcttcagc aagccgcgcc gcctgcgttc
     1561 caagaataag caggaacggc aatcccgacg aaaagccgag cgccagcatc aacgccagaa
     1621 cggggtctgt gacgatctgt cggaatttgg ggcggacttt catcggcgct cagtttaggt
     1681 tcgcgaagcg ccgaagcaag cgcggcgcct atgcggcttg cttcgcgccg ccgacgagaa
     1741 gaatttccgg cgcgccggcg gcgccgagtt ctgcacgcgc cgtctgcgtt gccgcatcat
     1801 cgcgctcgcc ggcggtcgcg aggacgacga agtcggcata aggcgccagt gccttcgtca
     1861 tcggattgct gggaaacggc ggcgcagcaa ggatgacgaa atcataggtc cgcgccaaag
     1921 cgtcgagcac aatatcgaga tcgtcggggt tgaaagtatc tttcgagccg aatggcacga
     1981 aatgcagccg cgacgcggcg tcgcgatgga ttacatcggc aaagctggcg tcgccgtcaa
     2041 gaagatcggt aaggcccatc atcttcccgt cctgcgcttc cggcccgatg agcttggcaa
     2101 gctggccgtc gcgggaatcg agatcgacga tgatggggcg cccgtcgcgc gccagcgtgc
     2161 gggcaaaact gatcagtgcg tccgttgcct ctttactttt attgaggcgg gtcgcgacaa
     2221 tgttgacgcc ctgcggcgtc gtggatttcg cggcagcctt ctcatccgat tcgtctggcg
     2281 cgggtgctgc gacgagttcc tcatcatccg actcggggac gcgcgcgctg gcaaagcgct
     2341 tcaaccgttc gattgtcgag accgccggac gcggcttctc ttctgccaag cgcaccggca
     2401 tcaccagcac gtcgtcgccc gtcgcatccg ataaggggcg atccgccaaa agctcgcggc
     2461 caacgacgat accaagagac agaacaaacg ctgcaagggt ggcgagaccg aggacttgcg
     2521 gcttcttggg gaacgaaggc tgatccggcg ccgtcgccgc agcgatgatg cgtgcatcgg
     2581 ccggcgtcgc accggactga cgggagacgg cctcctggta tttcgttgtc gcgctttgga
     2641 gctgctcgcg atagcccgct tcgacctgct ctagtgcgcg caactggacg ctattcgtat
     2701 tcgcggtctc cacagcctgt ttctgctgat cgagcgcggt ctggatattt gcgacgcgcg
     2761 aagcggcgat cttggaatcg ttttcaagcg agatcgcaat attgtcgccg gcaagccgca
     2821 attgcgcatt gagatcttca acctcggctt tcaattcgag aatgcgggga tgccgaggtc
     2881 cgagcgtacc cgcttcgagc gccaactggg cattcgccgt aacgcgctgc tcggagatgc
     2941 gacgaatcaa atcgttattg gcgacatctg gaatctcgga cacgcggccc ttgcgaagca
     3001 gttgccggat cagcgcggcc ttagcctggc tgttcgcctc gtccgtgcgg gcgcgcgtaa
     3061 gatctgtatt gaggtcggcg agttgctgcg cagtgatcgt catattgttc gcgccggcaa
     3121 gcaagcccga agccgcacga taacgttcga cctcatcgga agcgcgcgct aatttctcgt
     3181 tgagcgtatt gatctgacca ccgagtgcgt cggaaacgtc tttggccaga tcacgtttgg
     3241 catcagtttg ccgctccagg tagaccgcgg cgacctcgtt ggcgactttc gcagcaaata
     3301 ccggatcgtg cgcccaaaag tcgaccatca cgacctgggt cttgatgggt gagtaagccg
     3361 tcagcctttc cgaaaatgtc gcgatggtcc gatcttccgc ggagacccgc gtgggatcat
     3421 gcgccaatcc gaaaagcacc agcgtgcgct ggatgacccc aagccccttc gacaccggat
     3481 cgagctctgg attgccgacc agatgaagct tctcgatgac ctcctgcgcg acatcgcgcg
     3541 atgtgatcag cgaaacctga ctgccaatcc atgtctcatc gatcgctggc gcggtttcca
     3601 tcccctccgt gcgctgcgcg cgtgtgagat aactttcctg attctcgaga aggatttgcg
     3661 catctgcagt gtatcgcggt ttcgcgattg tgacgtaggc aagcgcaaga aggaacgcca
     3721 gaagcgtggg gatcaaaacc caatatttcc gggcgtaaag cgcggcgccg agcacgccga
     3781 gcgacatatc gtgcgacgcg tcgtccacca tcacgtcagg ctcgtatgcg tctttcatcc
     3841 gctgctccga agaccacaac cgaggcgcca agaccacacg cacgggcgtt ccgcgttcag
     3901 tatgaagctc ggttatggtt gaaacaatgt taatgtcacg aagaaaacac cgcccgagag
     3961 ttaagctttg ttaaccataa taaagtacga aagcttctac gaatctgcgg cttttaggga
     4021 atctccaagc ggtggcctcc gagatgcggc gcttctttgt ttcgttatgt ctcctcgtcc
     4081 tatgtcttgt gcttggggcc tgcgcggcaa caacttattc ggctgccctg gatactgatc
     4141 cgaatgcccc ctatacgctc gggagcggtg atcgtgtgcg cgtcatcgtg ttcggccagg
     4201 acagtttgag caactcctat gccattgacg gcgctggcca tatttcaatg ccattgattg
     4261 gctcggtcgg ggcgacagga gagaccacca cgtcgctcgc gcgtaagatc gaagcgcggc
     4321 tcagcgacgg ctttattcgc gatccgcacg tttcggtgga ggtcgaagcc ttcaggccgt
     4381 tcttcgttct gggcgaggtc acgacgcccg gccaatatcc ttatgttaac ggtatgactg
     4441 cagaaaccgc tgttgcgatc gctggcggct tcaccccgcg ggcctataag gatacagtcg
     4501 accttacgcg caccatcgat ggacatccgc tcaccgcgtc ggtcccgatt cttcacatga
     4561 tcaaacccgg cgatacgatc gtcgtgcgcg agcgatattt ttaaagaggc ctcaagccta
     4621 tgccgatgca gcaaatcgtg ccggagcgaa agctgcgcat cctgcatgtt tttcgcgcgc
     4681 ctttgggcgg cctctttcgg catgttatcg accttgcccg cgagcaagtt gcgcgcggac
     4741 acgcggttgg tgtgatcgcg gattcgctca ccggcggatc acgcgcgaat gaagtgctcg
     4801 gtgacctcgc gccatcctta gagcttggcc tttgccgtat cccgatgcac cggccggcgc
     4861 atatcctcga cgccgtcgcc gcagcgcgcg tcgcacgcca cgccgaccgg ctggatgtcg
     4921 atgtcgttca cggccatggt tcgaagggcg gcgcctatac gcgactgccc gcccttttgc
     4981 cgaccaaaag gaaagcgatc cgcgcctata cgcctcacgg cggcagtttc aattaccggg
     5041 gaggcccgct ggtcgagcgc ggctacatga tggcagagcg gctgcttact gccgggactg
     5101 acgtgttcct ttttgaaagc gcctttgtcg cccagcaatt tcgacgccgt gtcgggccga
     5161 cgaccaaact tacacggatc gtctacaatg ggctcagtcc gacggaattt ctgcccaccg
     5221 aaccacgcgc tgatgccgcc gaattcgtgt atattggcga attgcgcgcg gcgaagggaa
     5281 tcgacacact tatcgatgct ctcgcactgc tctccaaaac cggaatccaa ccgcgcctcg
     5341 tgctcgtcgg caccggtccc gacgaacgcg aactcatcac acgagccgag acgcgcggtg
     5401 tctccacaca ggtcactttc gcaggtctcg taccggcccg cctggcattt ccacttggcc
     5461 ggatactcgt cgtgccgagc cgcgcggaat ctctgcccta tattgtgctc gaggcaaccg
     5521 ccgcgcaaat tcctatcgtg gcaacagatg tcggcggcat caacgagatc ttcggtccgt
     5581 tccgcgatcg tctaatcgca cctgacgatc ctcaaatact cgccgacacg ttgcagaaaa
     5641 tgcttgctca ttccccggct gagtcaaggg cagacagcca ggctctcgcg agcttcgtga
     5701 agaccaaatt caaaatcagc gacatggcag attccgttct cgcgggctac gccgaggcga
     5761 tcgcaaaccg cgatcatcgc ggcgccacgg cggcgtcctt cgcccttccc tcttcatgag
     5821 accgcggata catggtgaaa ttctcgctgt ctgatctgca aaagactgag ccagtggaca
     5881 aggctgcgcc agccgccgac atgagcagcg aagcgctcgc cgctgcggca cgcgcgatgc
     5941 agagcagcag ctctatgccg ggcctttcgc gcgtcgttct cgaaggtctt gttcgctgtg
     6001 tcgaatttct gctgatcgcc gcagccggct ttgccataca tttcttttat atcgcgcgga
     6061 cgcagggcta tgcccagcct tatctgatcg gcgtgccgtg cgtcgcggcg gccgctgtgc
     6121 tcgctttgca aagtgccgcg ctttataaga tcagcgcact ccggcgcttt cccgctgaca
     6181 gtttccggct agccggtgcc ctcacgttcg tctttgccgc cacgctggtg ttggtcttct
     6241 tcttgaagct cgacgaggtt ttctcccgcg tctttatgct cgcctggtat ggcggcagcc
     6301 ttgttgcgct ttgcggcgaa cgcgccgcgc tggccttgct tgtcgatcgc ttgatccaaa
     6361 ccgggcgcct cgcccgccgt accgttctcg tcggcggcgg cacggcgggc gaggcgctgc
     6421 tcaatcaatt ggcggcacag ccacaacccg atctccagat tctcggcata ttcgacgacc
     6481 ggaccgacga gcgttcaccc gcggtcgtcg caggctttcc aaaattgggg acggttgacg
     6541 atctccttac cttcagccgc cgcacgcgca tcgatctaat catcttcaca ttgccgatca
     6601 ccgccgagca gcgcattctc cagatgttac gcaagctctg ggtgctgccg atcgatatcc
     6661 ggctcgcggc gcatatgaac aaactgcact tccgaccgca ttcctattcc tacatcggca
     6721 gcgttccgct gatcgatata ttcgacaagc cgatcgccgg ttgggacgtc atcgtcaagt
     6781 acgcattcga caaaatcgtc ggtgctctat gcctcatcct gcttgcgccg gtgatggcca
     6841 tcgtcgcgct ggcggtgaaa ctcgactcca aaggaccggc cctgtttcgt cagaagcgct
     6901 acggcttcaa caatgaaatc gtcgaagtct acaagttccg atctatgcgc acggacatgc
     6961 tcgatttcgc cgccacaaag ctcgtgacaa aggacgatcc gcgggtcacg cgcgtcggtc
     7021 gcttcatccg caagacctcc ctcgacgaat tgccgcaact ctttaacgtc gtcttcaagg
     7081 gcaatctttc cctcgtcggg ccgcgaccgc acgcgcttca cgccaaggcg gccgaacggc
     7141 aatatgacga agtcgtcgac ggctacttcg ctcgccatcg cgtgagcccc ggaattaccg
     7201 gctgggcaca gattcacggc tggcggggcg agacggatac gcccgaaaaa attcagggcc
     7261 gcgtcgagtg cgatctttat tacatcgaga actggtcgat cctgctcgac atctacattt
     7321 tgctcgcgac gcccttcgcg cttctgcgca cgaagaatgc atattgatct gacggatttg
     7381 gatcactgaa gcgcaaatcc taaaaatcgc tcgcgagtcc cgacattttc acaatcgggc
     7441 cgattcgaaa caaaacgcgc gccgtcttct ttcacgcagc cgatcagcgc catcgtacct
     7501 ggccgatgat gacgaaagac atatcccgcc gtccgacggc gtgcgctgcc ttccgccggg
     7561 caatcgccgg cctcggcgag aaaaccaacg catgtttgcg gcgagaaaca ttcatccaat
     7621 tccacgctgt cggcgtcatt gggagctgcc gtcatcaaat atcccaggcc gcgatcatag
     7681 gtgtaggaac gtccggccga aattgccggt cccctcgtcg tccaggtcat gtgctgcggc
     7741 gtgatccacc ttgccaaggc cgtccgcact tgcggagaac gaggcacagg agacagatga
     7801 atccggccct cctgaatgac cagatatctt tgcgtgaaat cctgtcctgg tggaatgtac
     7861 atataagcca ggaggaattt gtgcgagatg ttattgaggc ctttgtcggc gatcatcgcc
     7921 ggataagcat ataactcggt gggtgccggg cgtttccatg tgccggcgtc gtatggaatg
     7981 agcggctccg gaagcgtgtt gaagtccatt ttgtttttcg agaccgataa ttggagcgaa
     8041 gtgtcggcgg cgttcagcaa catgacagcg ccgatgctgg tccaataggc cgcgctcatg
     8101 ccagcctccg gaatcaacgg cgctgccatc cctcccagac ccggctcgtg ccagccgaca
     8161 ccgtcccatt ccagccattt tccgggggtc ggggcatcgt gcggcgcccg agcggcgatg
     8221 ttcttccagt cccgcaggcg cagacaatag gcgtaccaat aaccatcgtg gccatcgacc
     8281 gctgtgcagt ccccgacacc gctcggtccg ttctctacgt cgctttcatc gccggtgatg
     8341 atctgaccaa gtacattcca ggtcagcccc tcgtcgtgcg atattgcgat cgacattgat
     8401 ttttgcgtct cgcctgcgtt gtaattgcaa tgctgctcgt cgtgtatcat tccgtaaagg
     8461 aggtcgcgaa cgggcataag agtggtgagc cattgcccgc attcagacga cgagttctta
     8521 ggtccgggtt tgagaaccac tttcgaggtg ccgccgagat caagcggcgt cttaccgtcg
     8581 attgcgatgg tctccgcatt ggccgcaaaa ccacgaaatg tgccgttcgg tagctgaatg
     8641 atgctgatgg gtgcgtcagg ctcgttttgc gaaggtccgc gcaccacaat cggatgttcg
     8701 agctttagtt caatgcaagc agagcacaga tccgcgccgc atacggcgcc cgtgccacca
     8761 ataatcaaaa gagatgcaaa gaagcatttc caaccgaggc ccagccggcg gagatgtata
     8821 ggcgcgctat gatacaactg gcctagcatc gcccgaacgc tttgtggtcg cgacgacgcg
     8881 ccgtgattca tgagcgggac ctggaattgc agcgaagttt cgggggttga gcccccacaa
     8941 tacgccggtc aggagccaca taaacctcca cctctcccct tcgtgcagca tcgaacacga
     9001 aataatcgag agccaaccga agaatatcat caggcacagg ccattacgcg actgtttcca
     9061 ggtgttccag gcctgcatac ccgagagaat taagagaagc gtccaggcga gccccccgat
     9121 ccagccgaaa tagaggaaac aggctatcca cacattgtgg atgggctccg ggaaatagtt
     9181 ttcgatctga agcagcccaa gcccgaaagg gtgatcgagg atcatcggta tcgctagaag
     9241 atacctgttg tatcgcccga aatggccgag atcgtactcc tcagcgaccg taaagcgacc
     9301 cgataacatg tgcgatactg tgtcgttgag aacggcgaag agcagaacga cgacgaccag
     9361 cggcacgagg aatgcgagcg aagacagagt tgcccgcaat aattggtgcc tgttcaaaat
     9421 gcccagatat atcccgcccc agagcgccgc cgagacaatt gccgcacggg aaaaagatag
     9481 aaacacggga acagacaaaa gcaggagggc cgcggaaaac aaaatccgtc tgcgtttctc
     9541 agcaatcata aaaagcgagc ctaatatgcc cggaaccaaa aatacgccga acatatccgg
     9601 gtcatcgaga aatgcgcgcg gccggtcggc ccaggtgaga tcgggattgt gagccacaaa
     9661 tcccaaaatg ccgattaccg cggcgatgac gttggacagg atataaacat cgacgaatat
     9721 ccgaagctct cgctccgtcc acgcggttgc cacaaggcag cttgtaatgc cgataaacgc
     9781 aaccgacgtt agcgcaaaca tctcaaagac gacacgcgga tcggcggtca acgacatgga
     9841 cgatatataa accgatgaaa gccagaccag aatgaggcaa ataaagaaga acgttcttgt
     9901 atttatcaac tggttgcaga aaacggtcat cagcaatgcg ccgaagaaaa ggacatcgac
     9961 aggcgacggc cttgcccacg tgtagttgat tgcgataacg gccagaagga agatattcat
    10021 gcgccaccgc gtccaggccg cacgccggcg acttaaggcg gtaggcgggt cgccctcgac
    10081 tatcagtgcc ctgccgctct gcgagatggc caacgggaac tccaaaggtc caaggcgcgt
    10141 cgagcggccc atcaacgatc aaaagaggat ggcaggcgga ccgtagccct atttgatggc
    10201 agctactatc gaccgattcc ctcgcctcat ggcaagtttt tcagaggccg attaaaagac
    10261 ctttaaccat atacccgcgc tgcgggggag atgcatgatg tataagtggg cgaatatagt
    10321 gatcggactc gccgcgctga gcggcgcggc aggaatcatg gaatcggcgg cgagtgcgca
    10381 tacgatcgcc gacccgctgc tgaaaacatc cgcgaacatg ctcatcgtca acgccgcatc
    10441 ggtgattgcc atcagtggct atgcatccag ccgattgcag cattgggctc tgtttggcgc
    10501 cgccacgctc ctcgccggag gccttctgtt ttgcggcgaa ctcagcacgc acgtttttct
    10561 ggggcaaagg tcttttgctt tggcggcgcc tatcggcggc acgttgatga ttgccggctg
    10621 ggtgatcgct gcggcaagtg ccttc
//
LOCUS       DASWOG010000636         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_236348,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000636 DASWOG010000000
VERSION     DASWOG010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236348"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1128
                     /locus_tag="VGB93_13115"
     CDS             67..1128
                     /locus_tag="VGB93_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY0147056.1"
                     /translation="MPRRGRGAQSNASGRFEPLAYAPVDDGWAAEDELPPLKTSVTWE
                     RAKTIITRNESPDISFDRSINPYRGCEHGCVYCFARPTHAYMGLSPGLDFETKLFAKA
                     DAAALLERELAAKNYQPRTIAIGTNTDPYQPIERQHRVMRQVLEVLARTNHPVGIVTK
                     SALVLRDLDLLAPMAEKGLVKVALSVTSLDPLLARRLEPRAAAPERRLATIKALSEAG
                     VPTCVMVAPVIPALNDAEIETILERARAAGAREAGYVMLRLPLELSDLFNEWLVAHYP
                     GKARHVLSLIRSTREGKIYDSQFGKRMTGSGPYAWMVGRRFEAAAAKLGFAKSKVKLR
                     NDLFIPPKRVGDQLQLELL"
     gene            1214..1843
                     /locus_tag="VGB93_13120"
     CDS             1214..1843
                     /locus_tag="VGB93_13120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0147057.1"
                     /translation="MAQKSSLPHFRYERQLTKKSLWPVAGIDEVGRGPLAGPVAAAAV
                     ILDPARLPKGVNDSKALCAEEREAAYEEIMRHAVAVSVAFVSAAEIDTINIRQASLAA
                     MRRTLAALAVAPLYALVDGNDLPKALRCDGETIVGGDARSLSIAAASIVAKVTRDRLM
                     VRLCKLYPVYGFSRHVGYATPEHLAAIARHGPCPYHRLSFSPFRVVSAA"
     gene            1978..>2897
                     /locus_tag="VGB93_13125"
     CDS             1978..>2897
                     /locus_tag="VGB93_13125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0147058.1"
                     /translation="MRIARAGTAATKSQLKVLRTEHSRTLVPAVASPVVLKNRLPANE
                     ILVGDCLERLASLPAASVDLIFADPPYNLQLEHALSRPDQSAVDAVDDDWDKFASFAD
                     YDQFTRDWLAAARRVMKDNATIFVIGSYHNIFRVGTILQDLGFWILNDIVWRKANPMP
                     NFRGRRFTNAHETMIWAAKSASTKSYTFNYEALKAGNEDCQVRSDWFLPICTGGERLK
                     DETGSKTHPTQKPEALLARVLQAASLPGDLVLDPFFGTGTTGAVAKKLGRNFIGIERD
                     ETYAKAARARIAAVEPYPDETISAAPSKRT"
BASE COUNT          540 a          893 c          873 g          591 t
ORIGIN      
        1 ccgccctcgc ttcggccgcc gcgcgtcttc gattcacccc ccggaatcga ggacgcgcgg
       61 cctcttatgc cgcgacgggg ccgtggcgcg caatccaatg cgagcggccg ctttgagccc
      121 ttggcctatg cgcccgtcga cgacggctgg gcggctgagg acgaactacc gccgctcaag
      181 acctcggtga cgtgggagcg ggcgaaaacc atcatcaccc gcaatgaatc gcccgatatt
      241 tcctttgatc gctcgatcaa tccctatcgc ggttgcgagc acggctgcgt ctattgcttc
      301 gcgcggccga cgcacgccta tatgggcctt tcgccggggc tcgatttcga gacgaaatta
      361 ttcgctaagg ccgatgcggc ggctttgctc gagcgcgaac tggcggcgaa aaattatcag
      421 ccgcggacca tcgccattgg taccaatacg gacccctatc agccgatcga gcgccagcat
      481 cgcgtgatgc ggcaggtgct ggaagttctt gcccgcacca atcatcccgt cgggatcgtc
      541 accaaatcgg cgctcgtgct gcgcgatctc gatctcctcg cgccgatggc ggaaaaaggt
      601 ctcgtcaagg tcgctttgtc ggtaacgagt ctcgatccgc tgcttgcaag acgcctggag
      661 ccgcgcgcgg cggcgccgga aaggcggctt gcgacgatca aggcgctctc agaggcgggc
      721 gtgccgacat gcgttatggt cgcgccggtt attccggcgc tgaacgatgc cgaaatcgaa
      781 accattctgg agcgcgcccg ggctgccggg gcgcgcgaag ccggttatgt catgctgcgc
      841 ctgccactcg aattgagcga cctcttcaac gaatggctgg tggcgcatta tccgggcaag
      901 gcgcgccatg tcctttcgct catccgctcg acccgcgaag gcaagatcta cgattcgcaa
      961 ttcggcaagc gaatgacggg atcgggccct tatgcctgga tggtcggccg gcgtttcgag
     1021 gctgctgcgg caaagcttgg ttttgccaag tcgaaggtca agctgcgcaa cgacctgttc
     1081 atcccgccga aacgggtcgg cgatcaattg cagctcgaat tgttgtgagc atcgcatagg
     1141 gcggattgcg cgggtcctcg tctggagtct aagagcttgg ctcgggcggc attgccgctc
     1201 gcgaggctct cgaatggcgc agaaatcgtc tttgccgcat ttccgttacg agcggcaatt
     1261 gaccaagaaa tctctctggc cggttgccgg cattgatgag gtcgggcgtg gcccgctcgc
     1321 gggtccggtc gcggcggctg ccgtcattct cgatccggcg cggctgccca agggcgttaa
     1381 tgattcgaaa gcgctctgtg ccgaagaacg tgaggccgct tacgaagaaa tcatgcggca
     1441 cgcggttgcg gtgtccgtcg cctttgtcag cgcggccgaa atcgatacga tcaatattcg
     1501 gcaagccagc ctcgcagcca tgcggcgcac gctcgcggcg ctcgccgtcg cacctcttta
     1561 tgcacttgtc gatggcaacg atctgcccaa ggcattgcgc tgtgacggcg agaccattgt
     1621 tggcggcgac gcgcggtcgc tttcgatcgc agccgcctcg attgtcgcca aggttacgcg
     1681 cgataggttg atggtgcggc tctgcaagct ttatccggtt tacggattca gccgtcatgt
     1741 cggctatgcg acgccagaac atctggcagc catcgcgcgc catggcccgt gcccatatca
     1801 ccggctaagc ttctcgccct ttagagttgt gtcggccgct tagcgcttgg gcgtagctgc
     1861 aagcttcttt taatcaaatc gtacagaaaa gggacacgtt cggtttaccg acctaaacgt
     1921 ggattttacc ggcttcgcgc aaattgtcgt cgttcgtcgc gtatccggtg agtgtcaatg
     1981 cgtatcgcac gtgccgggac tgccgccacg aagtctcagc taaaggtctt gcgtactgag
     2041 cattcgcgaa cgctggtccc ggccgttgct tctccggtag tcctaaagaa tcgtcttccc
     2101 gccaacgaaa ttcttgtcgg cgattgcctt gagcggctcg cctcgctgcc cgcggcgagc
     2161 gtcgatctca tcttcgccga tccgccttat aatctgcagc ttgaacacgc attatcacgg
     2221 cccgatcaga gcgctgtcga tgcggtcgat gacgattggg acaagttcgc gagcttcgcc
     2281 gattacgatc aattcacccg cgattggctc gccgccgcgc ggcgcgtgat gaaggacaac
     2341 gcgacgattt tcgtcatcgg gtcctatcac aacattttcc gcgtcggcac gatcctgcag
     2401 gatctcggct tctggatttt gaacgacatc gtttggcgca aggccaatcc aatgccgaac
     2461 ttccgcggcc gtcggttcac caatgcccat gaaacgatga tctgggcggc gaagagcgcc
     2521 agcacaaaaa gctacacgtt caattatgaa gcgctcaagg ccggcaatga agattgccag
     2581 gtgcgttcgg attggtttct gccgatctgc acgggcggcg aacgcctcaa ggatgaaacc
     2641 ggcagcaaga cgcatccgac gcaaaagccg gaagccttgc tcgcgcgcgt gctgcaagcc
     2701 gcgtcacttc ccggcgatct cgtcctcgat ccgttcttcg gcaccggcac gacgggcgcc
     2761 gtcgccaaga agctcggccg caatttcatc ggcatcgaac gcgacgagac gtatgcgaaa
     2821 gccgcgcggg cgcgcatcgc cgcggtcgag ccctatccgg atgagacaat cagcgccgcg
     2881 ccaagcaaaa gaacgca
//
LOCUS       DASWOG010000637         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_125428,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000637 DASWOG010000000
VERSION     DASWOG010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125428"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..438
                     /locus_tag="VGB93_13130"
     CDS             37..438
                     /locus_tag="VGB93_13130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY0147059.1"
                     /translation="MPAFPFELVSPDRIVFSGEVEAVVVPGVEGEFTVLKDHAPTLAL
                     MKPGILEYDLSTTQKTKLFVRGGFAQIAPEGLTILAEQTIALEELDAAKLDADLKDAE
                     EDVADAKTDETRRRATERRDQISELKAALKL"
     gene            complement(448..1149)
                     /gene="msrA"
                     /locus_tag="VGB93_13135"
     CDS             complement(448..1149)
                     /gene="msrA"
                     /locus_tag="VGB93_13135"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0147060.1"
                     /translation="MSRYLMFGVVAATLGASAIWASSLSFAEPAAPVPAPLMDEAPRP
                     HLETAVVAGGCFWGVQGVFQHVKGVSEAISGYSGGSKANAHYEIVGTGQTGHAESVRI
                     TFDPRVVSYGKILQIYFSVATDPTELNHQGPDTGTQYRSTIFAANPEQGKVAAAYIAQ
                     LDKAHAFDAPVVTTIEPFRAFYPAEGYHQDFATLHPDDGYIAANDLPKIAALRQFFPA
                     LYRPHAKLVGATASE"
     gene            complement(1159..2940)
                     /locus_tag="VGB93_13140"
     CDS             complement(1159..2940)
                     /locus_tag="VGB93_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEY0147061.1"
                     /translation="MLLVIIAYLGGVLTILSPCILPVLPFVFARADEPFIKTGLPLLA
                     GMALTFAAVATLAALGGGWAVRANEIGRDAALVLLAIFGVTLLVPSIADRLTRPIVSL
                     GNRLSQSSTKEASSGQKIFSSFLLGIATGLLWAPCAGPILGLVLTGAALQGANAKTSL
                     LLLAYAAGAATSLAAALLIGGRVFAAMKRSLGAGEWIRRALGVAVLAGVAAIALGFDT
                     GFLTRVSAAPTNALEQVLIDKTRAQKPTTSVVMNDGAGAMMLSAHHHSSAATALPVEG
                     DMPSLAGATQWLNSPPLTREGLRGKVVLVDFWTYSCINCLRALPYVRAWADKYKDKGL
                     VVIGVHAPEFAFEKNIDNVKRAVRELGVTYPIAIDNDYAIWQGFNNEFWPAHYFIDAE
                     GRIRHHHFGEGEYAESEKVIQSLLAEANHRAIATDIVKVSARGAEAASDSADVQSPET
                     YIGYDRAEHFASTEDAVHDAPHVYALAPTLHLNQWGLTGNWTVGGERASLDTVGGKIT
                     FHFHARDLHLVLGPGKDGKPVHFRVLVNGQAPGADHGVDIDAAGNGTVTEQRLYQLVR
                     QSGPVQGRTFEIQFLDPGVEAFSFTFG"
     gene            complement(2950..3447)
                     /gene="msrB"
                     /locus_tag="VGB93_13145"
     CDS             complement(2950..3447)
                     /gene="msrB"
                     /locus_tag="VGB93_13145"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0147062.1"
                     /translation="MLTRRGFSMAGAAGLAAFGFSRLARGDAARAKRTYEVTHTDAEW
                     RQLLTPDQYDVLRQQGTERPFTSQLLDEHRAGIFACAGCDNHLFSSTTKFDSGTGWPS
                     FWAPLHNGVNEADDHTLGMVRTEISCHRCGGHLGHVFDDGPKPTGLRYCMDGVAMKFI
                     PGATA"
     gene            3580..>3728
                     /locus_tag="VGB93_13150"
     CDS             3580..>3728
                     /locus_tag="VGB93_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0147063.1"
                     /translation="MASPLLTLQSIRLSFGNMPLLSEAELSVTAGDRLCLVGRNGSGK
                     STLLK"
BASE COUNT          749 a         1132 c         1169 g          678 t
ORIGIN      
        1 ctggccgcac ggcgcgccta aaggggtaag aaaactatgc cggcttttcc ctttgaactg
       61 gtttcgcccg ataggatcgt gttttcgggc gaagtcgaag ccgttgtcgt gccgggagtc
      121 gagggcgaat tcaccgtgct caaggatcac gcgccgacgc tggcgctgat gaagccgggc
      181 atcctcgaat acgatctctc gacgacccag aagacgaagc tgtttgtgcg cggcggcttt
      241 gcgcagatcg cgccggaagg tctgaccatt ctcgccgagc agacaatcgc cctcgaagaa
      301 ctcgatgctg ccaagctcga cgccgatctc aaggacgcgg aagaagatgt cgccgacgca
      361 aagacggacg agacgcgccg tcgcgccact gagcgccgcg atcagatcag tgagctgaag
      421 gcggcgctga agctttaggc tcccgcctta ttccgaagcg gtcgcaccca ccagtttcgc
      481 atgcggccgg taaagtgctg ggaaaaattg gcgcagcgcg gcgatcttcg gcagatcgtt
      541 cgcggcgata tatccatcgt caggatgcag cgtcgcgaaa tcctgatgat agccttccgc
      601 cggatagaac gccctgaacg gttcgattgt cgtgacaacc ggtgcgtcga aggcatgcgc
      661 cttgtcgagc tgcgctatat aggccgcggc aactttcccc tgctcgggat tggcggcgaa
      721 aatcgtcgag cgatattgcg tgccggtatc cggcccctgg tgattcaatt cggtcgggtc
      781 ggtcgcgacc gaaaagtaga tctgcaagat tttgccatag ctgacgacgc gcggatcgaa
      841 cgtgatgcgc accgattccg catgacccgt ctggccggtg ccgacaatct cgtaatgcgc
      901 gttcgctttg ctgccgccgg aataaccgga gatcgcttcg ctgacgcctt ttacatgctg
      961 gaagacgcct tgcacgcccc agaagcagcc gccggcgacg acggccgttt caagatgcgg
     1021 gcgcggcgcc tcatccatca gcggcgcagg caccggggcc gcgggttcgg cgaagctcag
     1081 tgaacttgcc caaatcgccg acgctccaag cgtcgccgcg acgacaccaa acatcagata
     1141 tcgcgacata ttcgcctcct atccaaaggt gaacgagaaa gcctcgacgc caggatcgag
     1201 gaattggatt tcaaacgtcc ggccctgcac cggtccgctt tggcgcacga gctgatagag
     1261 ccgctgttcg gtcactgtac cgttgccggc agcatcgata tcaacaccgt ggtcggcgcc
     1321 cggcgcctgg ccgttgacca ggacacggaa atgaactggc ttgccgtcct tgcctggccc
     1381 aagcacgaga tgcagatcgc gggcgtgaaa atggaaggtg atcttgccgc ccacggtgtc
     1441 gagcgaggcg cgttcgcccc ccaccgtcca attgcccgtc agtccccatt gattgaggtg
     1501 caacgtcggc gccagcgcat agacgtgcgg ggcatcatgc accgcatctt cggttgacgc
     1561 gaaatgttcg gcacggtcat agccgatata ggtttcgggc gattgtacgt cggcggaatc
     1621 ggacgccgct tctgcgcccc gggcggagac tttcacgatg tctgtcgcaa tcgcccgatg
     1681 attggcttcg gcaagcaacg actggatgac tttttcggat tcagcatatt cgccttcgcc
     1741 gaagtggtga tggcgaatgc ggccttcggc atcgatgaaa taatgcgccg gccagaactc
     1801 gttgttgaag ccctgccaga tcgcataatc gttgtcgatg gcgatgggat aagtgacgcc
     1861 gagttcgcgc acggcacgtt tcacgttgtc gatgttcttc tcgaaggcga attccggcgc
     1921 atgcacgccg atgacgacga gacccttatc cttgtacttg tcagcccagg cgcgcacata
     1981 gggcagcgcg cgcaggcagt tgatgcagga ataggtccag aaatcgacga gtacgacttt
     2041 gccgcgcagg ccctcgcggg tgagcggtgg cgaattcagc cattgcgtcg caccggcaag
     2101 cgacggcatg tcgccttcga cgggcagggc cgtcgcggca ctcgaatgat gatgtgcgga
     2161 cagcatcatg gcgccggcgc cgtcattcat cacgacggag gtcgtgggtt tctgcgcgcg
     2221 ggtcttgtcg atcaaaacct gctcaagcgc gttggtcggc gccgccgaga cgcgggtgag
     2281 aaagcccgta tcgaagccga gagcaatggc agcgacgccg gccaggacgg cgacacccaa
     2341 tgcgcgccga atccactcgc ccgcaccaag cgagcgcttc atcgccgcaa agacgcggcc
     2401 gccgatgaga agagcggcag cgagcgaggt tgccgcgccg gcggcatagg ccagcagcaa
     2461 aagtgaggtt ttggcattgg cgccctgcaa tgccgcgccg gtgaggacga ggccaagaat
     2521 cggcccagcg caaggcgccc agagcagacc ggtggcgatg ccgagcaaga acgacgagaa
     2581 gattttctgc ccgctgctcg cttcttttgt cgaggattgc gagagtcgat tcccgagact
     2641 cacgatcgga cgtgtcagcc ggtctgcgat cgacggaacg agcaatgtca cgccaaaaat
     2701 ggcaagaagc acgagtgcgg cgtcgcggcc aatctcgttg gcgcgcaccg cccagccgcc
     2761 gccgagcgcc gccagcgtcg cgaccgcagc gaaggtaagc gccattccgg caagcagcgg
     2821 caggcccgtc ttaatgaacg gctcatcggc gcgcgcaaag acgaagggca acaccggcag
     2881 aatgcacggg ctcagaatcg tcaggacgcc gccgagatag gcgataatta cgagaagcat
     2941 gatgagacct caggccgtgg cgccgggaat aaatttcatg gcgacgccat ccatgcagta
     3001 gcgcaggccc gtcggcttcg ggccgtcgtc gaagacatga ccgagatggc cgccacagcg
     3061 gtggcaggaa atctccgtcc tcaccatgcc cagagtatgg tcgtccgcct cgttgacgcc
     3121 attgtgtagc ggcgcccaga agctcggcca gccagtgccg ctgtcgaact tggtcgtgga
     3181 cgagaagagg tgattgtcgc agccggcgca ggcgaagatt ccggcgcgat gttcatccag
     3241 cagctggctg gtgaacggcc gctccgtgcc ctgctggcgc aggacgtcat attgatccgg
     3301 cgtcagaagc tggcgccatt cggcatcggt atgcgtgact tcgtaggtcc gtttggccct
     3361 ggcggcatcg ccccgggcta accggctgaa gccaaaggcg gcaagtcccg ccgcgccggc
     3421 catggaaaat cctctgcggg tgagcatttt ggtttcctca gctccgaaag tggcggctcg
     3481 cgatttcggc cgtcattcgc taggagatac ggtcggaacc ggcgcgacgt tacgcccaat
     3541 gccggctctc acagacagtt gaaagattaa atctcgttca tggcctcccc ccttctcacc
     3601 ctccaatcca tccgcttgag cttcggcaat atgcccctgc tgagcgaggc cgagctctcg
     3661 gttaccgccg gcgatcggct ttgccttgtc ggccgcaatg gctcaggcaa atcgaccctt
     3721 ctcaagat
//
LOCUS       DASWOG010000638         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_236499,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000638 DASWOG010000000
VERSION     DASWOG010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236499"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGB93_13155"
     CDS             complement(<1..247)
                     /locus_tag="VGB93_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0147064.1"
                     /translation="MSEPKEKSKGLFGRLFKGGAASPDAAVPQKADVDVAASEVAQTP
                     FAEPVKQEPVRQGWFQRLRSGLGKTSSKLASGITDLFS"
     gene            complement(244..1506)
                     /gene="mtaB"
                     /locus_tag="VGB93_13160"
     CDS             complement(244..1506)
                     /gene="mtaB"
                     /locus_tag="VGB93_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY0147065.1"
                     /translation="MPEPQVITLGCRLNIYESEVMRRHARAAGLDDAIIVNTCAVTGE
                     AVRQAAQTVRRLRREKPDARIIVTGCAAQIEPERFAEMPEVDHVIGNAEKMQAETFSG
                     LSIADGARVQVNDIMSVRESASHMIDGFASQTRAYVQIQNGCDHRCTFCIIPYGRGPS
                     RSVGAGEVVTQVRALVEKDYAEVVLTGVDITSYGRDLPGPATLGKLVRQVLRHVPELK
                     RLRLSSIDQVEADEDLMIALRDEERLMPHLHLSLQAGDDLTLKRMKRRHLRSDAIAFC
                     EKARALRPDIVFGADMIAGFPTETDEMFENSYRIVDECGLTYLHVFPFSARRGTPAAR
                     MPQVVGTIVKERAARLREKGTTVLSSYLQSQRGRVVDVLVENGGAGRTPQFAEVMLQD
                     DGWRRAGAVVRTRITRVESQRLAGEACA"
     gene            complement(1563..>1773)
                     /locus_tag="VGB93_13165"
     CDS             complement(1563..>1773)
                     /locus_tag="VGB93_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0147066.1"
                     /translation="LTMACGSEACAASVCAERQRLTGRKVEVTLPGGILGIEWAANNH
                     ILMTGPVEFEYEGAIDFSKLAKVSV"
BASE COUNT          347 a          599 c          493 g          334 t
ORIGIN      
        1 tggagaaaag atccgtgatc ccggacgcca gcttcgacga cgtcttgccg agccccgatc
       61 tgagccgctg aaaccagccc tgcctgacag gctcttgttt gacgggctcc gcgaacggtg
      121 tctgcgctac ctcggacgcg gcaacgtcga catccgcctt ttgcggaacc gctgcatcgg
      181 gcgacgcagc accgcccttg aacaaacggc cgaaaagccc tttgcttttt tccttgggtt
      241 cgctcacgcg caagcctcgc cggcgagacg ctgcgactcg acgcgcgtga tgcgtgtccg
      301 aacgaccgcc ccagcgcgac gccaaccgtc gtcctgcagc atgacttccg cgaattgcgg
      361 cgtgcggccc gcgcccccgt tctcgacgag cacatcgacg acacggccac gctgggactg
      421 aagatacgaa ctgagcacag tggttccttt ttcgcgcaat cttgcagcgc gctccttgac
      481 gatcgtgccg acgacttgcg gcatccgcgc ggccggcgtg ccccgccgcg ccgagaatgg
      541 aaagacgtga agatacgtga ggccgcattc atccacgatg cgataggaat tctcgaacat
      601 ctcatcggtt tccgtcggaa atcccgcgat catgtccgcg ccgaatacga tgtcgggccg
      661 aagcgcccgc gccttctcgc aaaacgcgat ggcatcggaa cgcaagtgcc ggcgcttcat
      721 ccgcttcaac gtcagatcgt cgcctgcctg taatgacaaa tgcagatgcg gcatcaaacg
      781 ttcctcatcg cgaagcgcga tcatcaaatc ctcgtccgcc tcaacctgat cgatcgacga
      841 cagccgcaaa cgcttaagtt ccggcacatg ccttaatact tgccgcacca gctttccgag
      901 cgtcgcaggg ccgggaagat cgcgcccgta tgacgtgata tcgacgcccg tgagcacgac
      961 ttcggcataa tctttttcga ccagcgcgcg aacctgcgtg accacctcgc cggcgccgac
     1021 cgagcgcgag gggccgcgcc cataaggtat gatgcagaac gtgcagcgat gatcgcagcc
     1081 attttgaatt tgcacataag cgcgcgtctg cgaggcgaaa ccgtcgatca tatgcgacgc
     1141 ggattcgcga acgctcatga tatcgttgac ctgaacgcgc gcgccgtccg cgatcgacaa
     1201 tcccgaaaac gtctccgcct gcatcttctc ggcgttgccg atgacatggt cgacttccgg
     1261 catctcggcg aaccgttcgg gctcgatctg cgccgcgcat cccgtcacga taatgcgggc
     1321 gtctggcttc tcgcgccgca gccgccgcac cgtctgcgcc gcctgacgaa cggcttcccc
     1381 cgtcaccgcg caggtattga cgatgatcgc atcgtcgaga cccgccgcgc gcgcatgacg
     1441 gcgcataact tcggactcat atatattgag cctgcagcca agcgttatca cctgaggttc
     1501 gggcatcgac gtgatttttt ctctctgaaa tgcgctcgct tagccgtctc aggcaggtca
     1561 cactagacgg acactttcgc cagcttcgaa aaatcgatgg cgccctcgta ttcgaactcg
     1621 acgggaccgg tcatgagaat gtggttgttg gcggcccact cgatcccaag aatgccgccc
     1681 ggcaacgtca cctcgacctt gcgtccggtg agccgctgac gctccgcaca gacagacgcc
     1741 gcgcaggcct ccgaaccgca cgccatcgtc agt
//
LOCUS       DASWOG010000639         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_310122,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000639 DASWOG010000000
VERSION     DASWOG010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310122"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGB93_13170"
     CDS             <1..717
                     /locus_tag="VGB93_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147067.1"
                     /translation="ASNNLLDYEAARESATASRDSLANLLKDLSEIPDIGRIHILAHS
                     LGTWLTMEALRQRAIAGSPSLNGKLGLVMLAAPDIDLSVFRSQLSELDASHFSVLVSA
                     DDRALSLSRTLAGDRPRVGALDPRKPSDRAALARLGVKIYDLSGKSDGLIDHDAYADL
                     PDVVRSIGAQIGRQRIQDQDVTAVLGERPVDTQVSTAPLQSPSQPVAAAPAGSSTGSH
                     ALPQTPPTITPPAAASATAH"
     gene            complement(730..1134)
                     /locus_tag="VGB93_13175"
     CDS             complement(730..1134)
                     /locus_tag="VGB93_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147068.1"
                     /translation="MPRFWLRFCVFAALCAALASPAAARHHHKIATATGQQGFDGTYN
                     VEITTTDGHGSCPASYQGTLTVHDFRIVALSDPAATASGGIESDGTISLAFRKADQIA
                     NVGGQMKGSSGNGFWSSPTAFCGGLWRAARQN"
     gene            complement(1283..>1793)
                     /locus_tag="VGB93_13180"
     CDS             complement(1283..>1793)
                     /locus_tag="VGB93_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEY0147069.1"
                     /translation="IADSKGSEFLGKHMIPVILGPKERHYVIDHHHLGLALIEEKVKD
                     ILVNVVADLRTLTQEAFWVFLDNRAWMHPFDEDGQRKTYADIPKALTDLVDDPFRSLA
                     GELRRAGGYAKDTTPFAEFLWADFLRRRIKRKLVESDFTAAIEKALGFAKSKETDFLP
                     GWCGPNDGD"
BASE COUNT          349 a          552 c          540 g          352 t
ORIGIN      
        1 gcaagcaaca atttgttgga ctacgaggcg gcgcgagaaa gcgcgacggc ttcacgcgac
       61 tcacttgcca atctgctgaa ggatctgagc gagatcccgg acattggccg catccatatt
      121 ctggcccatt ctctgggcac ctggctcacc atggaagcgc tgcgccagcg cgcgatcgcc
      181 ggcagcccca gccttaatgg caagctcggc ctcgtgatgc tggcggcgcc ggacatcgat
      241 ctttccgtgt tccgctcgca gctttcagaa ctcgatgcat cgcatttctc cgttctggtt
      301 tcggccgacg acagggcgtt gtcgctatcg cggacgctgg ccggggatcg gccgcgcgtc
      361 ggtgcgctcg atccgcgcaa gccatccgat cgtgcggccc tggcccgtct cggggtgaaa
      421 atttacgatc tttcgggcaa atcggacggt ctgatcgacc atgatgccta tgccgatcta
      481 cctgacgtcg tgcgttcgat cggcgcgcag atcggccgcc aacgcattca ggatcaagac
      541 gtcaccgcgg tgctcgggga gcgcccggtc gatacgcagg tttcgacagc acccttgcag
      601 tcgccatcgc aacctgttgc cgcggcaccc gccggttcca gcacggggag ccacgcgttg
      661 ccacaaacgc cgccgacaat cacgccacca gcggctgctt cagcaaccgc gcattaaacg
      721 ggctcgcgtt taattttggc gcgcggcgcg ccaaaggccg ccgcaaaatg ccgttggcga
      781 agaccagaaa ccattgccgc tggagccttt catctggccg ccgacattgg caatctgatc
      841 agctttgcgg aacgccagag aaatcgtgcc gtcgctctca atcccgccgc ttgctgtcgc
      901 cgcaggatcg ctcagcgcga cgatgcgaaa atcgtggacc gttagcgttc cctggtagga
      961 cgccgggcag cttccatggc catccgtggt cgtgatctcg acgttgtacg taccgtcgaa
     1021 accttgctgg cctgtggccg tggctatctt atgatggtgg cgggccgctg ccgggctggc
     1081 aagcgcggcg caaagcgcgg caaaaacgca gaagcgaagc caaaagcgtg gcatgggaaa
     1141 ttccgatggg agcggcccac caagccagtt cggtggcgtt gccgcgaacc gcccgcgtga
     1201 gtcttgatgg cagaacccca gcttttgccg cagcgcgctc agaaatcaag agctttgctg
     1261 tggttgcaaa gctcctggac gcttaatcac catcattcgg gccgcaccag cccggcagga
     1321 aatccgtctc tttgcttttg gcgaaaccaa gagccttctc gatcgcggcc gtgaaatcgg
     1381 attcgaccag cttgcgttta atgcgccggc gcaggaaatc cgcccacagg aactcggcaa
     1441 agggcgtcgt gtctttggca tagccacccg cgcggcgcag ttcgccggca agcgagcgaa
     1501 acggatcatc gacaagatcg gtcagcgcct tcggaatatc cgcgtaggtc ttgcgttgtc
     1561 cgtcttcatc gaaaggatgc atccaggcac ggttatcgag gaagacccag aaagcttcct
     1621 gcgtgagggt gcgcagatcg gcgacaacat tcacgagaat gtctttgacc ttctcctcga
     1681 tcagggcgag gcccagatgg tgatggtcga tgacgtaatg gcgttccttc ggcccgagaa
     1741 tgaccgggat catatgtttg ccgaggaatt ccgaaccttt cgagtcggcg att
//
LOCUS       DASWOG010000640         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_345179,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000640 DASWOG010000000
VERSION     DASWOG010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345179"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1682
                     /locus_tag="VGB93_13185"
     CDS             <1..>1682
                     /locus_tag="VGB93_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEY0147070.1"
                     /translation="NIDATDKTLQQVETALQQPGLSKDVLQKLQEQIGPLGPTLQGVL
                     DHLTPHLAAQKAQLDQLGAAPAAKALPESKEVAAERVKRQKAFDDVDALVKRTNLLIV
                     QVDQAQTTIASRLRARLTEALLQQEPSIASPSLWADVIREVPRNLHALRHRIGGWLSD
                     SATKLTGWRAPVFCGLLILVAVLYWPLTQFAQRTFFRKHVADNPTPLQKILAAWRVLF
                     VIAAIPIATALFIGFLARNFGLIDDQNPLLRPFFFAVVRISIGAGLAQALLAPKREQW
                     RLIAVDSDAAAHVLRTVLIVTVLVSCGRIFNAIGSTIEASWSSDMFARGFFSLLAAIA
                     IAASLWIGQSGDEEESEQVFGPRVDQTANWFSVLRTILWAAILVVFAAVLAGYMALGR
                     FAIDQIIWVGGLGGIAVMLVMLIDQLVGQGITGTTPFGRRMMLSTGLSREAFAQFAIL
                     ASGVARVVIFLIAVLAALVPWGVQSADVTGYLYAAFFGFRIGDVTISLSRIVFTLVIF
                     ATGYAATSDVERWLEVSFLPPTRLDLGLGNAIKTIIGYIGRIFALGFALAYL"
BASE COUNT          278 a          524 c          510 g          370 t
ORIGIN      
        1 cgaatatcga tgcgacggac aagactctgc agcaagtcga aacggccttg cagcagccgg
       61 gactttcaaa agacgttttg cagaaattgc aggaacagat cgggccgctc ggcccgacat
      121 tgcagggcgt gctcgatcac ctgaccccgc atctcgctgc acaaaaagcg cagctcgatc
      181 aactcggcgc ggcgcccgct gccaaggcgc ttccggaaag caaggaggtc gcggccgagc
      241 gggtgaaacg gcaaaaggcc ttcgatgacg tcgatgcgct ggtcaaacgc accaatctgc
      301 tcattgtcca ggtcgatcag gcgcagacga ccattgcgtc ccggctgcgc gcccggctga
      361 ctgaagcact gctgcaacag gagccgagca tcgcgagccc gtcgctgtgg gccgacgtga
      421 tccgggaagt tccgagaaac ctgcatgcgc tgcgtcaccg gatcggcggc tggctcagcg
      481 acagcgccac caaactcacc ggatggcgcg ctccggtatt ttgcggattg cttattctcg
      541 ttgcggtttt gtattggccg ctgacgcaat ttgcgcagcg gacttttttt cgtaagcacg
      601 tggcagacaa tccgacgccg ttgcagaaaa tcctggcggc ctggcgcgtt ttgtttgtca
      661 tcgcagcgat cccgatcgcc actgcgcttt ttatcggctt ccttgcgcgc aacttcggac
      721 tgatcgacga tcagaatccg ttgctgcgac cgttcttctt cgctgtcgtg cgcatttcca
      781 tcggcgccgg gctcgcacag gcgctgctcg cgcccaaacg cgagcaatgg cggcttatcg
      841 ccgtcgattc cgacgccgcg gcacacgtgc tgcgcaccgt tctgattgtt acggtgctcg
      901 tttcctgcgg ccgcatcttc aatgcgatcg gcagcaccat cgaggcgtcc tggtcgagcg
      961 acatgtttgc gcgcgggttc tttagcctat tggccgcgat cgcgattgcc gcaagcctct
     1021 ggatcggcca aagcggcgac gaagaagaaa gcgagcaggt tttcggtccg cgcgtcgacc
     1081 agacggcgaa ctggttttcc gttctgcgca ccatcctctg ggcggcgatt ctcgtcgtct
     1141 tcgccgcggt cctcgccggc tatatggcgc tcgggcgttt cgcgatcgac cagatcatct
     1201 gggtcggcgg tctcggcgga atcgcagtca tgctcgtgat gctcatcgat caacttgtcg
     1261 gccagggcat caccggcacg acgccattcg gccgccggat gatgctttcg accggattga
     1321 gccgcgaggc gttcgcgcaa ttcgcgatcc ttgcttccgg cgtcgcccgt gtggtgattt
     1381 tcctcatcgc cgtgctcgcg gcgctggtgc cgtggggcgt ccaatcggcg gatgtgacgg
     1441 gctatctcta cgccgcattc ttcggtttcc ggatcggcga tgtcacgatt tcgctgtcgc
     1501 gcatcgtctt cacgctggtc attttcgcca ccggttatgc cgcgacgagc gacgtcgagc
     1561 gctggctcga agtgagcttt ctgccgccta cgcggctcga tctcgggctg ggcaacgcca
     1621 tcaagacgat tatcggctat attgggcgca tttttgcgct tggctttgcc ctggcctatc
     1681 tc
//
LOCUS       DASWOG010000641         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_216921,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000641 DASWOG010000000
VERSION     DASWOG010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216921"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /gene="acs"
                     /locus_tag="VGB93_13190"
     CDS             <1..1798
                     /gene="acs"
                     /locus_tag="VGB93_13190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0147071.1"
                     /translation="FTKVKNTSFGPGEVSIKWFEDGETNVAINCIDRHLKTRGDQVAI
                     IWEGDDPAEAEHITYKQLHERVCRFANALKAQGIESGDTVTIYLPMIPQAAYAMLACA
                     RIGAIHSVVFGGFSPDSLAGRIRDCSSKLLITADEGLRGGKKVPLKSNVDAALEKAPD
                     VVKTVIVVKLTGGDVKWRDGRDLWYHDLVEEASADCPATPVDAEHPLFILYTSGSTGT
                     PKGVVHTTAGYLIYASMTHQYVFDYHDGDIYWCTADVGWVTGHSYILYGPLANGATTL
                     MFEGVPTYPSISRFWEIVDKHQVNIFYTAPTAIRSLMGAGEAPVKKTSRASLRLLGTV
                     GEPINPEAWEWYYRVVGDSRCPIMDTWWQTETGGHLITPLPGVTPLKPGSATKPFFGI
                     RPEIVDGSGQVQEGAAEGILVIADSWPGQMRTIYRDHERFVQTYFASFPGKYFTGDGA
                     RRDADGDYWITGRVDDVINVSGHRMGTAEVESALVAHDHVAEAAVVGFPHDIKGQGIF
                     AYVTLVNGREPSEKLRKDLVAWVRQEIGPIASPDIVQFAPGLPKTRSGKIMRRILRKI
                     AENEFHALGDTSTLADPAVVDDLISERKKHSL"
     gene            1944..2477
                     /locus_tag="VGB93_13195"
     CDS             1944..2477
                     /locus_tag="VGB93_13195"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0147072.1"
                     /translation="MSTLSLRIGVAPALFACVAAVLSVAGPALAARRDVVFSSGYPAG
                     EIIISQRQRALYFTEGDGRAIRYPVAIGKRGMAWLGSAEVEGKYMAPDWSPPAIVAHD
                     HPELPNFIPGGSPHNPMGAAAITLSRYQVAIHGTTQHMRRSIGSAASYGCIRMYNEDV
                     VDLYQRVNVGTPVLAVP"
BASE COUNT          478 a          800 c          767 g          486 t
ORIGIN      
        1 tttcacgaag gtcaagaaca cctcgttcgg tcctggcgaa gtctcgatca aatggttcga
       61 ggacggcgag accaatgtcg ccatcaattg catcgaccgg catctcaaga cgcgcggcga
      121 tcaggtcgcc atcatctggg agggcgatga cccggcagag gccgagcaca tcacctacaa
      181 gcaattgcat gagcgggtct gccgcttcgc caatgcgctc aaggcgcaag gcatcgaaag
      241 cggcgacacg gtcacgatct atctgccgat gatcccccag gccgcttatg cgatgctcgc
      301 ctgcgcgcgg atcggcgcca tccattccgt cgtcttcggc ggcttttcgc ccgattcgct
      361 ggcgggccgg attcgcgatt gcagctccaa actgctgatc accgccgacg aaggcctgcg
      421 cggcggcaag aaagtgccgc tcaaaagcaa tgtcgacgct gcgttggaaa aggcgcccga
      481 tgtcgtcaaa accgtcatcg tcgtcaaact caccggcggc gacgtcaaat ggcgcgacgg
      541 gcgcgacctc tggtatcacg accttgtcga ggaagcctca gccgattgcc cggcaacgcc
      601 ggtcgatgcc gagcacccac ttttcattct ctacacgtca ggttcaaccg gcacgccgaa
      661 aggcgtcgtt cacacgaccg ccggctacct catctacgcg tcgatgacgc accaatatgt
      721 cttcgattat catgacggcg acatctactg gtgcacggcc gatgtcggct gggttaccgg
      781 gcacagctat attctttatg gcccgctcgc caatggcgcg acgacactga tgttcgaagg
      841 cgtcccgacc tatccgtcga tctcgcggtt ctgggagatc gtcgataagc atcaggtgaa
      901 tattttctac accgccccga cggcgatccg ctcgctgatg ggcgcgggcg aggcgccggt
      961 gaagaagacg agccgcgcat cactgcggct tctcggcacg gtcggcgagc cgatcaatcc
     1021 ggaagcctgg gaatggtatt acagggtcgt cggcgacagc cgctgcccga tcatggatac
     1081 atggtggcag acggagactg gcggccattt gatcacgcca ttgcctggcg tgacgccact
     1141 caagcccggc tcggcaacca agcccttctt cggcatcagg ccggagatcg tcgatggcag
     1201 cggccaggtg caggagggcg cggccgaagg cattctcgtc atcgccgatt cctggcccgg
     1261 ccagatgcgc accatctacc gcgaccacga gcggttcgtg cagacctatt tcgcgtcctt
     1321 ccccggcaaa tatttcaccg gcgacggtgc gcggcgtgac gccgatggcg attattggat
     1381 caccggccgc gttgacgatg tgatcaacgt ctccgggcat cgcatgggca ccgccgaagt
     1441 cgaaagcgcg ctcgttgccc acgatcatgt tgccgaggcc gcggtcgtcg gcttcccgca
     1501 cgatatcaag ggtcagggta ttttcgccta tgtcactttg gtgaatggcc gcgagccgag
     1561 cgaaaaactg cgcaaggatc tggtcgcctg ggttcggcag gaaatcggcc ctatcgcctc
     1621 gcccgacatc gtacaattcg cgcccggcct gccgaagacg cgctccggca agatcatgcg
     1681 gcggatttta cgcaagatcg ccgagaacga attccatgcc ttgggcgata catcgacttt
     1741 ggccgacccg gccgtcgtgg acgatctcat cagcgagcga aagaaacact cgctttaggt
     1801 taagaacaaa ctcttgaggt gtgctgcggc ccgcgggcca cacctcaaga ggattttaag
     1861 acgcgcctca caatgtcagc tccagcgccg cagcccttgc tagggtcgcg gcaggcggag
     1921 tttgttgcgt aagggggcgc gtcatgagca ccttgagttt gcggatcggc gtcgcaccgg
     1981 ccttgtttgc ctgtgttgcg gccgttttga gcgtcgccgg acctgccctt gcggcacgcc
     2041 gggacgtcgt cttctcgtcg ggatatccag ccggcgaaat catcatcagc cagcgccagc
     2101 gcgcacttta cttcactgag ggcgacggcc gcgctatccg ctacccggtc gcgattggca
     2161 agcgcggtat ggcgtggctc gggtcggccg aggtcgaagg caaatatatg gcgccggatt
     2221 ggtcgccacc ggcgatcgtc gcgcacgacc acccggaact gccaaacttt atccctggcg
     2281 gctcgccgca taacccgatg ggcgccgccg cgatcacgct gtcgcgctat caggtcgcga
     2341 tccatggtac gacgcagcac atgcgccgct cgatcggcag cgccgcctcc tatggctgca
     2401 tccgtatgta taacgaagac gtggtcgatc tctaccagcg cgtcaatgtg ggcacgccgg
     2461 ttctcgccgt tccttaatcc tgtcggcttg tgccgtcctg ccctatgatg cgcgcgggag
     2521 gctgacatgg a
//
LOCUS       DASWOG010000642         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_363328,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000642 DASWOG010000000
VERSION     DASWOG010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363328"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1072)
                     /locus_tag="VGB93_13200"
     CDS             complement(167..1072)
                     /locus_tag="VGB93_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0147073.1"
                     /translation="MPVHEPELDPPFEIIDPSRLTSPLVLSSPHSGSVYPAEFLASSR
                     LDAATLRRSEDAYVDELFMGGVRSAAPLIRARFPRAYLDLNREPYELDPRMFETRLPS
                     FANTRSVRVAGGLGTVARVVGDSQEIYAHKLRLDEVLARIETLYKPYHRALETLLSRA
                     WQAFGMAVLIDCHSMPSVSTCPPGRESERRVKADFVLGDRYGTSCAGDLIDLVDHELR
                     RMGYTVFRNKPYAGGFITEHYGDPVNHIHTMQIEINRGLYMNEANLAKGPRFESIARD
                     LSNVVAALAAHVASGHSGTVRAAAE"
     gene            1320..1706
                     /locus_tag="VGB93_13205"
     CDS             1320..1706
                     /locus_tag="VGB93_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0147074.1"
                     /translation="MNDNSFQPNQKILLAEDDNDMRRFLAKALQNAGYAVVSFDNGLS
                     AYNRLREEPFELLLTDIVMPEMDGIELARRATELDPEIKVMFITGFAAVALNPDNHAP
                     RQAKILSKPFHLKDLVNEVNRLLAAA"
BASE COUNT          425 a          578 c          578 g          369 t
ORIGIN      
        1 gccgcacaac ccacaaatag gtcaataaga cttgcgcttt gtgcgacgca cgtccatatt
       61 attgcggtgc gacaacccgg ttgtcgccga tgccctcctt gggcgtttcc tccctagact
      121 tgggccgctt gcttcattgc aagcggcctc tttcttttca ggtgttctat tcggccgcag
      181 cgcggaccgt gccagaatgg ccggatgcga catgcgcggc aagcgccgcg acaacgttcg
      241 acaagtcacg ggcgatcgac tcgaaacgtg ggcctttagc gaggttcgcc tcattcatgt
      301 aaagcccgcg attgatctca atctgcatcg tatggatatg attcaccggg tcgccgtaat
      361 gctcggtgat gaaaccgccg gcatagggct tgttgcggaa gaccgtatag cccatgcggc
      421 gcaattcgtg gtccacgaga tcgatgaggt cgccggcgca actcgtgccg tagcggtcgc
      481 ccaagacgaa atccgctttg accctgcgct cgctttcacg ccccggcggg catgtcgaga
      541 ctgacggcat cgaatgacaa tcgatcagga ccgccatccc gaatgcctgc caggcgcgcg
      601 acagcagagt ctcgagcgcg cgatgatacg gcttataaag cgtctcgatc cgggcgagca
      661 cttcgtcgag gcgcagctta tgagcgtaga tttcctgcga atcgccgacg acgcgggcca
      721 cagtgccaag accgccggcc acccgcacgg agcgggtgtt ggcaaagctc ggcaggcgcg
      781 tctcgaacat gcgcggatcg agttcataag gctcgcgatt gagatcgaga tacgcgcgcg
      841 ggaagcgcgc gcgtatcaac ggcgcggcag accggacgcc gcccatgaaa agctcgtcga
      901 cataggcatc ttccgaacgc cgcagggtcg cggcatcgag ccgcgaactc gccagaaatt
      961 ccgcgggata gacggagccc gaatgcggcg acgagagaac cagcggcgac gtcaggcgcg
     1021 aggggtcaat gatttcgaac ggcggatcga gttccggctc atgcacggga atgtcccacg
     1081 cgagcggcaa agacgaaagg ttgccgggaa taaatgaggc cattaatgct ccagtaaggg
     1141 ccgccaacgc cggccaaagc atccgcgtcc ggggagtttg acaccagacg atcaatctgt
     1201 cgatgcactg ggtgccggtc aaatctccgc gaaaacgacc gctacccact cattatacga
     1261 tcattttcac tcgattttta ccggatcggg ctttgataga cggtaaatca ggggcaccga
     1321 tgaacgacaa ttctttccag ccaaatcaga agatcctgct cgccgaggac gacaacgaca
     1381 tgcgtcgctt cttggccaag gcgctgcaaa atgccggcta tgccgtcgtt tccttcgaca
     1441 acggcctctc ggcctataac cggctgcgcg aggaaccgtt cgagcttctc ctgaccgata
     1501 tcgtgatgcc cgagatggac ggtatcgagc tcgcccgccg cgcgaccgaa ctcgacccag
     1561 aaatcaaagt gatgttcatc accggcttcg ccgctgtggc gctcaacccg gacaaccatg
     1621 cgccgcgaca ggcgaaaatc ctgtcgaagc ccttccatct gaaagacctc gtgaacgagg
     1681 tgaatcggct gctcgcggca gcgtgaaacg ccgcacatcg acagcttgcg cggcgtgcgc
     1741 gaagccgata taggcttcgc ttcccgcggc cgagaggccg tatcttaagg ggcgcgtagc
     1801 tcagcgggag agaactacgt tgacataata gggttggctt tagcaattac ttgcggttag
     1861 ccaatccttt ctggttcaag tgaaccaacg ggcggttagc tcagtggtag agcatcgtgt
     1921 tgacatcgcg agggtcactg gttcgatacc
//
LOCUS       DASWOG010000643         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_328143,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000643 DASWOG010000000
VERSION     DASWOG010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328143"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGB93_13210"
     CDS             complement(<1..348)
                     /locus_tag="VGB93_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147075.1"
                     /translation="MSSLNWPVLQRIGVLLPLLGLGSCIEEVRPLYGSGSFFANGAQA
                     EKMQSVAVDEIGGRLGHYLGDDLQLDLNGTGAPTDPQYHLVVTLTEGTETPLIDTVEG
                     LATSATIVTTANFR"
     gene            complement(335..>2140)
                     /gene="leuS"
                     /locus_tag="VGB93_13215"
     CDS             complement(335..>2140)
                     /gene="leuS"
                     /locus_tag="VGB93_13215"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0147076.1"
                     /translation="AAAHDPALRDFIAECQRKGTSEAAIETAEKRGYDTGLKVRHPFD
                     ADWLLPVYVANFVLMDYGTGAIFGCPAHDQRDLDFANAYGLGNTPVVCPLGTDPAVFV
                     ITDKAYDGDGTLINSRFLDGLSIDAAKEDVSRRLEAATLGKEPVAKRQVNYRLRDWGI
                     SRQRYWGCPIPIIHCKKCGVVPVTAADLPVVLPSDVSFDQPGNPLERHPTWKHVACPQ
                     CGGDARRETDTMDTFVDSSWYFLRFTDPWDKAEPTDAAAVKKWLPVDQYIGGIEHAIL
                     HLLYARFFTRAMQETGHADALKEPFAGLFTQGMVVHETYRDANGGWVSPSEIRVDLTP
                     EGRRAARLDNGASVEIGPIEKMSKSKRNTIDPDEIIETYGADTARWVVLSDSPPEGDV
                     IWTDAGVQGAAKFVQRAWRLVDELAGIAARLGTPAPTEFSAAALDVRKLTHAALVKVE
                     EDIQRLRFNRAVAQIYDLANKLSTAVGAIESPEIGDDLRFAFREAADIFVQLFAPMMP
                     HLAEESWVRLGHATLVAEAPWPIADRSLIVENMMSLPVQVNGKKRADLVITRDADAAT
                     IEKAAVALEGVQKALEGKAVKKVIIVPQRIVNVVA"
BASE COUNT          397 a          668 c          669 g          406 t
ORIGIN      
        1 gcggaaattt gccgtcgtca caattgtcgc tgacgtcgcc aggccttcca ccgtgtcgat
       61 cagcggcgtt tctgtccctt ccgtgagcgt cacgacgagg tggtactgtg gatcggtcgg
      121 tgcaccggtg ccgttgagat cgagctgcag atcgtcgccg agataatgac caagacggcc
      181 gccaatttcg tcgaccgcga ccgattgcat tttctcggcc tgcgcgccgt tggcgaaaaa
      241 gctccctgag ccatagagcg gccgcacttc ttcgatgcag ctgccgagtc cgagcaaggg
      301 caggagcacg ccgatgcgct gcaagacggg ccagttaagc gacgacattg acaatccttt
      361 gcgggacgat gatgaccttc ttcaccgcct tgccttccag cgccttttgc acaccctcga
      421 gcgccacggc cgccttttcg atcgttgcgg catcggcgtc gcgggtgatg acgagatccg
      481 cgcgcttctt gccattcacc tgcaccggca aactcatcat attttcgacg atcaggctgc
      541 gatccgcaat gggccatggc gcctcggcaa ccaaagtcgc gtgaccgagg cggacccagc
      601 tttcctccgc cagatgcggc atcatcggcg caaatagttg cacaaaaatg tctgccgcct
      661 cgcggaaggc gaaacgcaga tcgtcaccga tctccgggct ttcaatcgcg ccgacggcgg
      721 tcgagagttt atttgcgaga tcgtagatct gggcgacggc gcgattgaag cgcaggcgct
      781 ggatatcttc ctcaaccttg accagagccg catgcgtcag cttgcgcaca tcgagggccg
      841 cggcggaaaa ttccgtcggt gcgggcgtgc caaggcgggc ggcgatgccg gcaagctcgt
      901 cgacgaggcg ccatgcgcgc tggacgaatt tcgccgcgcc ctgcacgccg gcatcggtcc
      961 agatcacgtc gccttccggc ggcgaatcgg agagcacgac ccagcgggcc gtatcggcgc
     1021 cgtaggtttc gatgatctcg tcggggtcga tcgtgttgcg tttcgacttc gacatcttct
     1081 caatcgggcc gatctcgacc gacgcgccat tgtcgagccg cgccgcgcgg cggccctccg
     1141 gcgtcaaatc gacccgaatt tccgaagggg agacccagcc gccgttcgcg tcgcgatagg
     1201 tctcatgcac caccatgccc tgcgtgaaga gccccgcgaa cggctccttc agcgcatcgg
     1261 cgtggccggt ctcctgcatg gcgcgggtga agaagcgtgc gtaaagcagg tgcaggatcg
     1321 cgtgttcgat accgccgata tattgatcga ccggcagcca tttcttcacc gccgcagcgt
     1381 cggtcggctc ggccttgtcc cacgggtcgg tgaaacgtaa gaaataccag gacgaatcga
     1441 cgaacgtgtc catcgtatcc gtctcgcgcc gtgcgtcgcc gccgcattgt gggcaagcga
     1501 cgtgtttcca ggtcgggtgg cgttcgagcg ggttacccgg ctggtcgaag ctgacgtccg
     1561 agggcaatac gaccggcaga tcggcggcgg tcacgggcac gacgccacat tttttgcaat
     1621 ggatgatggg gatcggacag ccccaatagc gctggcgcga aatgccccag tcccgcaggc
     1681 gataattgac ctggcgcttg gcaaccggct ccttgccaag ggtcgcagct tcgaggcgcc
     1741 tcgacacgtc ctctttcgcg gcgtcgatgc tcaacccatc gaggaagcgc gaattaatca
     1801 gcgtgccgtc gccgtcatag gccttgtcgg taatgacgaa caccgcggga tcggtgccaa
     1861 gcggacagac cacgggagta ttgccgaggc cataggcatt ggcaaaatcg agatcgcgct
     1921 ggtcgtgagc cgggcagcca aagatggcgc cggtgccgta atccatcagc acgaaattgg
     1981 cgacatagac cggcagaagc caatcggcat cgaagggatg gcgcaccttg aggccggtgt
     2041 cgtagcccct tttttcggcg gtctcgatgg cggcttccga cgtgcctttg cgctgacact
     2101 cggcgatgaa atctcgcaac gctggatcat gcgccgccgc
//
LOCUS       DASWOG010000644         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_180959,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000644 DASWOG010000000
VERSION     DASWOG010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180959"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VGB93_13220"
     CDS             <1..731
                     /locus_tag="VGB93_13220"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0147077.1"
                     /translation="DDFAAGTRMNEVAEELLCLVAYPEQTKSANASKCWNWFNPGDQQ
                     RDRGEPSLIADVTRQIMRDYAIDSARVFVAGLSAGGAAAAIMGHEYPDLYKGMCVHSG
                     LACGSAKDMASAFAVMQSGGSKVSAQSPSKLAIPTIVFHGSADKTVSPSNADQVVQQS
                     KSGATTSAVTSHGRSMGGIEFTCTVETDAAGDVILEQWTLHGAGHAWSGGSKKGSFTD
                     PRGPDASREMMRFFAEATKTHPLA"
     gene            complement(733..1335)
                     /locus_tag="VGB93_13225"
     CDS             complement(733..1335)
                     /locus_tag="VGB93_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0147078.1"
                     /translation="MRVSKEKAAQNRNALLEAASRLFRRGGIDGVGVADIAREAGLTH
                     GALYAHFPSKDALAAEAFCHGFNVNMAGFLASTGGRRLSFEEHLDGLFSPDRRDSVET
                     GCPLGASVSEIGRQSCAISGSFTRAFEEMIAMIERSLDDRLPASERRRLAVATVTAQI
                     GAIAVSRGVAKTDMALSDEALQAVRETIDAALRAPTASIA"
     gene            1450..2496
                     /locus_tag="VGB93_13230"
     CDS             1450..2496
                     /locus_tag="VGB93_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147079.1"
                     /translation="MRYKLFGEHTGLRVSELVLGAGNFGTLWGYGAEPDEARRIFDAY
                     AVAGGNFIDTANGYQFGQSEEIVGDLLSGRRDDFVLATKFSVRTDMNSGILVTGNSRR
                     AMVASVEASLKRLKTDRIDLYWVHMSDGVTPVEEIVRGFDDLVRAGKIVYAGLSDFPA
                     WRVARAVTIAELRGAAPIAGLQVEHSLVERSTEQELLPAGHALGLGIVAWSPLGGGVL
                     TGKYRQGEKGRAEGFGGKVFQAENTPQRTAILDTLIAVAKDAGVTPSEIAIAWVAAKG
                     ALPIIGPRTLAQLENNLGATKVVLSTDQIARLDKVSAIPAGFPYTVLNDPWTRELFTG
                     GKLKQFDAPTATVA"
BASE COUNT          508 a          752 c          748 g          488 t
ORIGIN      
        1 cggatgactt tgccgccggc acgcgaatga acgaagtcgc ggaagagctt ctctgtcttg
       61 ttgcctatcc ggaacaaaca aagtctgcca atgcctccaa atgctggaat tggtttaacc
      121 caggtgacca gcagcgcgat cgcggcgaac catcgctcat tgctgacgtc acgcgtcaga
      181 taatgcgcga ctacgccatc gattccgcac gcgtgtttgt tgcgggtctc tcagccggcg
      241 gcgctgccgc ggcaatcatg ggtcacgagt acccagatct ttacaagggc atgtgcgtcc
      301 attccgggct cgcctgcggg agcgcgaaag acatggcctc ggcgttcgcg gtcatgcaat
      361 ccggcggctc aaaggtaagc gcacaatctc catcgaaact ggccattccg acaatcgtat
      421 tccacggcag cgccgacaag actgtgagcc cgtctaatgc tgatcaggtc gtccagcaaa
      481 gcaaatcggg cgcgaccact tcggcggtta caagtcacgg tcgatcaatg ggaggtatag
      541 agtttacctg caccgttgaa actgatgcag ccggcgatgt catcctggag cagtggaccc
      601 tgcatggcgc tggtcatgct tggtcaggtg gaagcaagaa ggggtcgttt acagatccac
      661 gcgggccgga cgccagccga gaaatgatgc gtttctttgc agaagcgacg aagacacacc
      721 ccttggcctg agttatgcta tgctggctgt tggggccctg agagcggcat cgatcgtctc
      781 gcggacggcc tgcaatgcct catctgaaag cgccatatcc gttttggcca ctcctcgcga
      841 gactgcgatc gcaccgatct gtgcggtaac ggttgcgaca gccagccgcc gcctttcaga
      901 cgcgggaagc cgatcatcga gcgatctctc aatcatcgca atcatctctt cgaaagcacg
      961 ggtaaagctg ccgctgatag cgcagctctg ccgtccgatc tcgctgacgg acgcccccag
     1021 cggacatcct gtttcgacgc tgtcgcggcg atccggtgag aagagaccgt caaggtgttc
     1081 ctcgaatgat aggcgccggc ctcccgttga cgccaggaac ccagccatat tgacgttgaa
     1141 tccgtggcag aaggcttcag ccgccagcgc gtccttcgac ggaaaatgcg cgtagagggc
     1201 gccatgggtc agcccggctt cccgcgcaat gtccgcaacg ccgactccgt cgataccgcc
     1261 ccgacggaag agacggctcg cggcctccaa caaggcgttc cggttctgcg ccgccttctc
     1321 cttggaaaca cgcatctcct cagctccaaa cttcttccga gatttttagt tgcgagcata
     1381 atcaaagtca aatagacgct tcgttggcgc tttgattgtg atctctatca aaatgtggag
     1441 aaactgccca tgcgttacaa gcttttcggc gaacatacgg gactgcgggt ttccgaactg
     1501 gttcttgggg ccggaaattt cggcacgctg tgggggtatg gcgcagagcc cgacgaagcc
     1561 cgccgaatct tcgatgcgta tgcggtcgcc ggtggcaatt tcatcgatac cgcgaatggt
     1621 tatcagttcg gccagtccga ggagatcgtg ggcgatctgc tcagcgggag acgcgacgac
     1681 ttcgtactcg cgacgaagtt cagcgtcaga accgatatga actcaggtat cctcgttacc
     1741 ggcaacagcc gcagggcgat ggtcgcgtcg gtcgaggcga gcctgaaacg gctcaagacg
     1801 gatcggatcg acctctactg ggtccatatg tcggatggcg tcacgccggt cgaggagatc
     1861 gtgcgcggct tcgatgatct cgtccgcgcc ggcaagattg tctatgcagg tctttccgac
     1921 tttcccgcat ggcgcgtggc gagggccgtg accatcgccg aactgcgcgg cgctgcgccg
     1981 atagccggtc ttcaggtcga acatagcctc gttgaacgca gcaccgagca ggagcttctc
     2041 cccgccggtc acgcgcttgg cctcggaatc gtcgcctggt cgccgctcgg cggcggcgtg
     2101 ctgaccggca aataccgcca gggtgagaaa gggcgcgccg agggttttgg tggcaaggtg
     2161 tttcaagccg aaaatacgcc gcaacgcacc gccattctcg atacgttgat cgcggtcgcc
     2221 aaggatgccg gcgtgacgcc gagcgaaatc gccatcgcct gggttgcggc caaaggcgcg
     2281 ctgccgatca tcgggccgcg cacattggcg cagctcgaaa acaacctcgg cgcgacaaag
     2341 gtggttctgt caacggacca gatcgcgcgc ctcgataagg tcagcgcgat cccggcaggt
     2401 ttcccctaca cggtactcaa cgatccgtgg acccgcgagc tatttaccgg cggcaagctc
     2461 aagcaattcg acgctccgac ggcaaccgtc gcgtaa
//
LOCUS       DASWOG010000645         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_328336,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000645 DASWOG010000000
VERSION     DASWOG010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328336"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VGB93_13235"
     CDS             <1..1008
                     /locus_tag="VGB93_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0147080.1"
                     /translation="FDPISGHWSHTGAIFNFPAAFVIVLMSALMVVGIRDSARANNVI
                     VAIKLTIIILFIVIGAFYFNKANWITSTNPHGAFIPPALGPGHYGWGGVLRGAGVVFF
                     SYIGFDAISTAAQEAVDPKRDMPRSILGSLFFCMVLYALVGLVITGIIPYDRLNVPDP
                     IAVGVDHIGLGFFAPIVKLGAILGLSSVILVLLLGQSRIFYSIARDGLLPPLAARVHP
                     KFRTPYITTITTGIIVAVLAGLAPIGLVGELVSIGTLFAFVVVCIGVMILRYKHPNIE
                     RPFRTPAVNIVGPLGAAVALLLMIGLPLDTWIRLAVWLVIGLAIYFLYGRKHSHLNKG
                     V"
     gene            complement(1012..>1732)
                     /locus_tag="VGB93_13240"
     CDS             complement(1012..>1732)
                     /locus_tag="VGB93_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0147081.1"
                     /translation="LAGGAEKPRDLPIPGRDLAGIHFAMDFLPQQNRRVSREPFHTNQ
                     PILAAGKRVIVIGGGDTGSDCIGTSVRQGAVSVTQLEIMTMPPEKENKLLTWPDWPLK
                     LRTSSSHEEGAKRDFAVMTKEFRGENGIVKQLVCVHLDDKMKEIPDSTFTIEADLVLL
                     AMGFVAPIYEGLLEKLGVKLDGRGNVEANVKNYKSSLDKIFACGDMGRGQSLDVWAIR
                     EGRQAAQSVDEFLMGSSVLPR"
BASE COUNT          300 a          540 c          481 g          411 t
ORIGIN      
        1 ttcgatccga tcagcggaca ttggtcgcat accggcgcaa tcttcaattt tcccgccgct
       61 ttcgtcatcg ttctgatgag cgcgctgatg gtcgtcggca ttcgcgactc ggcgagagca
      121 aataatgtga tcgtggccat caagctcacg atcattattc tcttcattgt gatcggtgcg
      181 ttttacttca acaaggcgaa ttggatcacg tccaccaatc cccacggcgc attcataccg
      241 ccagcgctgg gtccgggaca ttatggctgg ggcggcgtgc tgcgcggcgc cggcgtcgtt
      301 ttcttttctt atattggctt cgatgcgatc tccacagcgg cgcaagaagc ggtcgatcca
      361 aagcgcgaca tgccgcgctc gatcctcggc tcgctcttct tctgtatggt cctgtatgcg
      421 ctcgtcggcc tcgtgatcac cggcatcatt ccctacgaca gactgaacgt gccggatccg
      481 atcgccgttg gcgtcgatca catcggcctc ggcttctttg cgccgatcgt aaaactcggc
      541 gctattctcg gcttaagctc cgttattctg gttctgttgc ttgggcagtc gcgcattttc
      601 tactcgatag cgcgcgatgg gcttctgcca ccgttggcgg cccgcgtgca tccgaaattt
      661 cgaacgccct atatcacgac gatcacgacc ggcatcatcg tcgccgttct cgctggcctc
      721 gcgccgattg gcctcgttgg cgaactcgtc tcgatcggca cactcttcgc cttcgtcgtt
      781 gtctgcatcg gcgtgatgat tttacgttac aaacacccga atatcgaacg gccgttcaga
      841 acgcctgccg taaacatcgt cgggccgctg ggtgccgccg tcgcgctgct gttgatgatc
      901 ggcctgccgc ttgatacttg gatccggctc gccgtctggc tagtgatcgg gctcgcgatc
      961 tattttctct acggacgcaa gcacagccac ttgaacaagg gcgtttagtc cctatcgcgg
     1021 caggacgctt gagcccatca ggaattcgtc gaccgattgt gcggcctgac ggccttcgcg
     1081 gatcgcccag acgtcgagcg actgcccgcg gcccatgtcg ccgcacgcga agattttatc
     1141 caacgaggac ttatagttct tcacattggc ttcgacattg ccgcgcccat cgagcttgac
     1201 cccgagcttt tccaacaggc cttcatagat cggcgcaacg aaccccatcg cgagcaatac
     1261 gagatcggct tcgatggtga atgtgctgtc cgggatttct ttcatcttgt cgtcgagatg
     1321 gacgcagaca agctgcttga cgattccgtt ctcgccgcga aattccttgg tcatcacggc
     1381 gaagtcgcgc ttggcgcctt cttcatgact cgacgaggtg cgcagcttca gcggccaatc
     1441 cggccacgtc agaagtttgt tttccttctc cggcggcatg gtcatgattt caagctgcgt
     1501 gacgctcacg gcgccctgac gcaccgacgt gccgatacag tcggaaccgg tgtcaccgcc
     1561 gccaatgacg atgacgcgct tgccggccgc aaggatcggc tgattggtat ggaacggctc
     1621 gcggctaacg cggcggttct gctgcggcag aaaatccatc gcgaagtgaa tgcccgcgag
     1681 atcgcggccg ggaatcggca gatcgcgcgg cttctccgcc ccgcccgcga gc
//
LOCUS       DASWOG010000646         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_88771,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000646 DASWOG010000000
VERSION     DASWOG010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88771"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VGB93_13245"
     CDS             complement(<1..168)
                     /locus_tag="VGB93_13245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY0147082.1"
                     /translation="MAWTRDQMAERAAKELRDSYYVNLGIAIPTLVANFIPAGMHVQL
                     QSENGMLGMGPF"
     gene            complement(179..886)
                     /locus_tag="VGB93_13250"
                     /pseudo
     CDS             complement(179..886)
                     /locus_tag="VGB93_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            complement(981..1754)
                     /gene="tam"
                     /locus_tag="VGB93_13255"
     CDS             complement(981..1754)
                     /gene="tam"
                     /locus_tag="VGB93_13255"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEY0147083.1"
                     /translation="MSAWDPLLYLRYGDERGRAAHDLLARVPIDQASLVYDLGCGPGN
                     STELLVQRFPDAEIVGVDSSEEMLGRARAVLPDIDFRTSDLRQWRPERPADLLFANAV
                     FQWIPNHLDIFEALIDAAPPGGVLAVQMPDNLAEPAKTLMRETAAEGRWSAKLAGVRE
                     TRKLLPAPEVYYDRLRPLCRSFELWHTVYHHVLENPAAIVEWMTGSGLRPYLALLDAA
                     EKRDFLDAYTAAIASIYPPLADDRVMLHFPRFFFVAVRT"
     gene            complement(1768..1971)
                     /locus_tag="VGB93_13260"
     CDS             complement(1768..1971)
                     /locus_tag="VGB93_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY0147084.1"
                     /translation="MQLYMVPKMSCDGCVRTIESAVKSLDPGAKVSCDLKTREVAVDT
                     RELPALVAEALAAVGYNSTLVSA"
     gene            2026..4077
                     /locus_tag="VGB93_13265"
     CDS             2026..4077
                     /locus_tag="VGB93_13265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0147085.1"
                     /translation="MTIAPSDTSPPPVDVNLKIEGMSCASCVGRVEKALARVPGVVSA
                     NVNLARESARVGINAGAVATEDLTKAVEKAGYSARLEEPLIPGQTHPHHGAHHDMAGM
                     SGMGDMRHGGVSRNQLLLAIVLTVPVLLMAMVPDMVPAVDNYIRTNLGIFPWRVTEAL
                     LTTVVLFGPGRMFFTTGIPALLRGGPEMNSLVALGTFAAWAYSMFATFLPQVFPAGTA
                     NVYFEAAAVISTLVLLGRYLEARARGEAGAAVEHLLRLAPDSATVLRDGAPVQVKLDT
                     IVPGDILLIRPGDRVPLDGEVIEGSSLVDESMLTGESRPVNKTVSAQVFGGTVNANGS
                     LTVRVTKVGADTVLARIVHMVGEAQGAKLPVQALADKVTAWFVPAVIVVALLTFVAWL
                     IFGPHPAIAYALVNMVSVLIVACPCAMGLATPVSVMVATGRSAQLGILFRDATALQTL
                     RDVKLVAFDKTGTLTEGHPALTDIDVALGFDANHVVAVAAAVESLSEHPVGKALVAAA
                     AARKLSLLKATDFQATPGFGVTAIVDGQKVSIGSGRFMAKLGFDISVFDTSATKLSET
                     GKSALFVAIDQSAAAILAVADPVKASAAAAIAALKKSGVGLAMVSGDRQATADAVART
                     LGIDKVEAEVLPDGKVAAVKTLQSQAGKIAFVGDGINDAPALAAADVGIAVGTGTDSA
                     I"
     gene            4093..4344
                     /locus_tag="VGB93_13270"
     CDS             4093..4344
                     /locus_tag="VGB93_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147086.1"
                     /translation="MMRDDLGLVASAIDLSRATLRNIEQNLFWALAYNVILIPVAAGV
                     LYGFGHILLSPMLAAGAMAFSSLFVVGNALRLRRFQPRV"
     gene            complement(4341..>5458)
                     /locus_tag="VGB93_13275"
     CDS             complement(4341..>5458)
                     /locus_tag="VGB93_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0147087.1"
                     /translation="RAEDGATRWISARGSFFRGDGQIQRILCLCSDITGRKRSELRNA
                     QLAAIVASSIDAIVSIDFDDVIRTWNYGAEQLFGYPAKDVIGKSGMFTVPPELKDERR
                     AMMDRLMNGEAVEYQTRRLDRAGHSMDVWIRGAPVRSVEGNLFGASLIIRDITAQKKR
                     EEHVRFLMRELTHRSKNLLAVIQAMARQSLSLQTDPKDFVGRFSERLSSLAGSHDLLS
                     NDDWAGASLTQLIRSQLQHFGDLLDSRILIEGADIILKPEAAQNIGIALHELSTNAAK
                     FGSLSGETGTVTVSWSIDPDEGEASRMHLFWRERGGPPVEAPDHRGFGRTVMERVAGQ
                     ALGGQSKATFAPSGVIWELDVPATSVVREKTPEPAAG"
BASE COUNT         1025 a         1637 c         1689 g         1107 t
ORIGIN      
        1 aaatggcccc atgccgagca tgccgttctc gctctgcaat tggacatgca tgcctgcggg
       61 aatgaaattg gcgacgagcg tcggaatggc gatgccgaga ttgacgtaat agctgtcgcg
      121 caattctttc gcggcgcgct cggccatctg atcgcgtgtc caagccatga ttatttcctc
      181 aggctgcggc gcgcggacgc gtcgtgagtt tctcgatggg cttcgccgga tttggcgcat
      241 agacgatacg ctggacgaag atacctggcg tatggatatc gtcgggatcg atttcgcccg
      301 gctcgaccag atgctcgact tccacgaccg taagtttgcc ggcggtcgcc atcatcggat
      361 tgaaattgcg cgccgtcttc cggaagacga ggttgccttc cttgtcggcg ttgcaggcgt
      421 gaacgagcga aatgtcggca acgagggcgc gttccatcac ctaggtctcg ccgtcgaagt
      481 ccttgtgctc cttgccttcg gcaatgatcg tgccgacgcc ggtcttggtg aaaaacgcag
      541 gaatgcccgc gccgccggcg cgaatacgct ccgccagcgt gccttgtgga ttgaattcaa
      601 gttcgagttg accggacaga aattgctcgg cgaaaagctt gttttcgccg acataggacg
      661 acaccatttt cttgatctgc ttcgtctcca gcaggatgcc gagaccgatg ccgtcgacac
      721 cggcgttgtt ggagatcacc gtcaaatctt tggcgccaag ttcgtggatc gcctggatga
      781 ggcttgtcgg gatgccgcag aggccgaagc cgccggccat cagcgtcatg ccgtctttga
      841 gaacatcggc gagcgcactg cgcgcatccg ggaaaagctt gtccatcctc cgcagagcct
      901 cttatgtcgt cggggcggag gcattcgtaa gccgccccat gccgcaatgc aagaaggccc
      961 gctcttagac gaaagtccaa tcaggtccgg acggcgacga aaaagaagcg ggggaagtgc
     1021 aacatgacgc ggtcgtcggc gagcggcgga taaatggacg cgatcgcagc cgtataggca
     1081 tcgagaaagt ctcttttttc ggctgcgtcg agcagtgcga ggtaaggccg cagcccggat
     1141 cccgtcatcc attcgacaat cgcggcggga ttttcgagga cgtgatgata gaccgtgtgc
     1201 caaagctcga aactgcggca gagcggccgc agacggtcat aataaacttc gggagcgggc
     1261 aggagcttgc gcgtctcgcg gacgccggca agcttcgccg accaacgacc ttccgcggca
     1321 gtttcgcgca tcaacgtctt ggccggctcg gccagattat cgggcatctg gacggcgagg
     1381 acgccacccg gaggggcggc atcgatcaac gcctcgaaaa tatcgaggtg gttaggaatc
     1441 cattggaaga cagcattggc aaatagcaaa tctgccggcc gttcgggccg ccattggcgc
     1501 aaatcggagg tacggaaatc gatgtccgga aggacagcgc gggcgcgccc gagcatctct
     1561 tccgaactat cgaccccgac gatctcggca tccggaaacc gttggaccag aagctccgtg
     1621 gaattgcctg gcccgcagcc gagatcatag acgaggctcg cctgatcaat cggcacgcgg
     1681 gcgaggaggt cgtgcgccgc acggcctcgc tcgtcaccat aacgcaaata gagcagggga
     1741 tcccaggccg acacaacagt ggcgtcctta cgcgctgacc aatgtgctgt tgtaaccgac
     1801 ggcggccaat gcctcggcga cgagcgcggg aagttcgcgc gtatcgacgg caacttcacg
     1861 cgtcttgaga tcgcaggaaa ccttggcacc cggatcgagc gatttcaccg cgctttcgat
     1921 cgttcgcaca cacccatcgc agctcatttt tggaaccata taaagttgca taactccctc
     1981 tcccaaagcc ttgtgcgtgg tcaccccgca atcatatacg taaacatgac cattgctccg
     2041 tcagatacaa gccccccacc ggttgacgta aatctgaaga tcgaggggat gtcgtgtgcg
     2101 tcctgcgtcg gccgcgtcga aaaggcgctg gcgcgcgtgc cgggcgtggt ctctgccaat
     2161 gtgaatctcg cccgcgagag cgctcgcgtc ggaattaacg caggcgcggt ggctacagag
     2221 gatctgacca aagccgtcga gaaagcgggc tattcggcgc ggctggaaga gcctctcatc
     2281 cccggacaaa cgcacccgca tcacggcgcc catcatgaca tggccggtat gtccggcatg
     2341 ggtgacatgc gtcacggtgg ggtgagccgc aatcaacttt tattagcgat cgtactcact
     2401 gtccctgtgc tgctgatggc gatggtgcct gatatggttc cggcggtgga taattacatc
     2461 cgcacgaatc tcggcatctt cccttggcgg gtaaccgagg ccctgctgac gactgtcgtg
     2521 ctttttggtc ccggccgcat gttttttacg acgggtattc cagcgctttt gcgcggcggt
     2581 ccggagatga actcgctcgt cgcgctcggg actttcgcgg catgggctta ttcgatgttc
     2641 gctactttcc tgccgcaggt gtttcctgcg ggcactgcga atgtctattt cgaggctgct
     2701 gctgttatct cgacgctcgt gcttctcggc cgctatctcg aagcgcgtgc acgcggcgag
     2761 gcaggagctg cggtcgaaca tctgcttcgg ctcgcgccgg acagtgcgac tgtgttgcgt
     2821 gacggtgccc cggtccaagt caaactcgat acgattgtcc cgggtgatat cttgttgatc
     2881 cgccccggcg atcgcgtgcc gctggacggc gaggtcattg aaggttcgtc tctcgtcgat
     2941 gaatccatgc tgaccggcga gtcgcgaccg gtgaacaaaa cggtcagtgc gcaagtcttc
     3001 ggcggcacgg tcaatgcgaa cggcagcctg acggtgcgcg tcaccaaggt cggcgccgat
     3061 acagttttgg cgcgcattgt ccacatggtc ggcgaggcgc agggcgcgaa attgccagtt
     3121 caggccctag ccgacaaagt cacggcctgg ttcgtgccgg cggttatcgt cgtggcgctg
     3181 ctgacgttcg tcgcatggct catcttcggt ccgcatccgg cgatcgctta tgcgctcgtc
     3241 aacatggtct ccgtgctcat cgtcgcgtgt ccttgcgcca tgggccttgc gacgcccgtg
     3301 tccgtcatgg tcgcgacggg acgcagcgcg cagctcggca ttctcttccg cgatgccacg
     3361 gccttgcaga cgttgcgcga cgtgaagctt gtcgccttcg acaagaccgg cacgctgaca
     3421 gaggggcatc cggcgttgac ggatatcgac gttgcgctgg gctttgacgc gaaccatgtc
     3481 gtcgccgtcg cggcggcggt cgaatctctc tccgaacatc cggttggcaa ggcgctggtc
     3541 gcagcagcag ccgcgcgcaa gctcagtctt ctcaaagcga cggattttca ggcgacgccc
     3601 gggtttggtg tcaccgccat tgtcgacggc cagaaggtca gcatcggctc cggccggttc
     3661 atggcgaagc tcggcttcga catttcggtt ttcgatactt cggcgaccaa attgagcgaa
     3721 acgggaaaat cagcgctttt tgtcgcgatc gatcaaagcg ccgccgcgat ccttgccgtt
     3781 gcggatcccg tcaaagcctc ggccgctgca gcgatcgccg ccctcaagaa gagtggcgtg
     3841 ggcctcgcca tggtcagcgg cgatcggcag gcgacggcgg atgctgttgc ccgcaccctc
     3901 ggcatcgata aggtcgaggc ggaagttctg ccggatggca aggtggctgc ggtcaagact
     3961 ctgcaatcgc aggccggcaa gatcgccttt gtcggtgacg gcatcaatga tgcgccggcg
     4021 ctggcggcgg ccgacgtcgg gatcgcggtc ggcacgggaa ccgacagtgc tatttagacc
     4081 gcggatgtcg ttatgatgcg cgatgatctg ggtctggtcg caagtgcgat cgatctcagc
     4141 cgcgcgacac tgcggaacat cgagcagaat ctattctggg ccttggccta caacgtcatt
     4201 ctcatcccgg ttgcagctgg cgttctttac ggcttcggcc atattctgct ctcgccgatg
     4261 ctcgctgccg gggcaatggc gttctcgtcg ctcttcgtcg tcggcaatgc actgcggctg
     4321 cgccgcttcc agccgcgggt ttagcctgcc gccggttcgg gcgttttctc gcgcaccacg
     4381 gacgtcgccg gaacgtcaag ctcccagatg acgccgcttg gcgcgaacgt cgccttggat
     4441 tgtccaccca aggcctgccc ggcgacacgt tccatcactg tgcggccaaa accccggtga
     4501 tcgggcgctt cgaccggcgg tccgccgcgc tcccgccaaa acaggtgcat tcgcgatgcc
     4561 tcaccctcgt ctggatcgat cgaccaggac acggtgacgg tcccggtctc gccggagagc
     4621 gagccgaact tcgccgcatt ggtcgaaagt tcgtgcagcg cgatgccaat gttctgtgcc
     4681 gcctccggct tcaggatgat gtcggcgccc tcgatcaaaa tgcgtgagtc caagagatcg
     4741 ccgaagtgct ggagctgcga gcgaatgagc tgcgtcagcg aggcgccggc ccaatcgtca
     4801 ttggatagaa gatcatgcga gcctgcgagg ctcgacaggc gttcgctgaa ccggccgaca
     4861 aagtccttcg gatcggtctg cagcgagagc gattggcgag ccatcgcctg aatgacggca
     4921 agcagatttt tcgagcgatg cgtcagctcg cgcatcagga agcgcacatg ctcctcgcgc
     4981 ttcttctgcg ctgtgatgtc gcgaatgatg agcgacgcac caaagagatt gccctcgaca
     5041 ctacgcaccg gcgcgccgcg aatccagacg tccatggagt gtcccgcgcg gtcgagacgc
     5101 cgcgtctgat attcgacggc ctcaccattc atgagccggt ccatcatggc gcgtcgctcg
     5161 tctttaagtt caggcggcac agtgaacatg ccactcttgc caataacgtc cttcgcggga
     5221 tagccgaata attgttcggc gccataattc caggtgcgaa taacgtcgtc gaaatcgata
     5281 ctgacgatgg cgtcgataga cgaggcgaca atcgctgcaa gctgcgcgtt gcgcaattcg
     5341 ctgcgcttac gcccagtgat gtcagagcac aggcagagaa tgcgctgaat ttggccgtcg
     5401 ccccggaaaa agctgccacg tgcactgatc cagcgtgtcg caccatcttc agcacggg
//
LOCUS       DASWOG010000647         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_125812,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000647 DASWOG010000000
VERSION     DASWOG010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125812"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..484)
                     /locus_tag="VGB93_13280"
     CDS             complement(83..484)
                     /locus_tag="VGB93_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0147088.1"
                     /translation="MIGMVLVTHGHLATEFRAALEHVVGPQKQIATVSIGPEDDMEER
                     RKDILAAVKQVDRGIGVVVLTDMFGGTPSNLAISVMNGGQVEVVAGINLPMLIKLASI
                     RDTTSLEQAVLQAQDAGRKYIYVASRVLGGK"
     gene            complement(701..1171)
                     /locus_tag="VGB93_13285"
     CDS             complement(701..1171)
                     /locus_tag="VGB93_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147089.1"
                     /translation="MSAEAIAPVSIHASGVVVGEAGIVIRGVSRIGKSSLALALLDAA
                     AQAALFGALIGDDRLLLSASYGRLIERGHPAIRGQIEQRGVGILEIGAEPAAVVRLVV
                     DIFPPEQMLRYPEAINPSVTLCDVDLPLLALAKIGSASDSARTVLARLRQFGAI"
     gene            complement(1168..>2561)
                     /locus_tag="VGB93_13290"
     CDS             complement(1168..>2561)
                     /locus_tag="VGB93_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEY0147090.1"
                     /translation="VLRRLVSPTRTRARIYDRDGYLLLDSRALSARGDILRLDLPTQS
                     DEAGNWLERVWAAMKRSFTYNELPLYEDIGIANGRTYPEVSRALDGQAQSIVRMNNKG
                     ETIVSVAVPIELLRTVRGALLLSTQGGDIDKIIASERWAIIRVFLVSAAIMLLLSLLL
                     AGTIAGPMHRLAEAAERVRRGIKSRQEIPDFTNRSDEIGHLSGALRDMTRALYHRMDA
                     IESFAADVAHELKNPLTSLRSAVETLPIARTDDSRARLLAIIQHDVRRLDRLISDISD
                     ASRLDAELARADMEPVDLSRIILTVVTMANHVEHESVQVTLDLANNEDAKKNGFIVLG
                     HDSRLGQVFNNLIDNAKSFSQPGGSVRVGLRPAKSALTNGLLTDGFEIVVDDDGPGIP
                     ADAFERIFERFYTDRPNHGFGQNSGLGLSISRQIIEAHGGGIRAMNRITTGRDGSPIV
                     LGARFIVWIRAAR"
BASE COUNT          513 a          786 c          796 g          466 t
ORIGIN      
        1 tgcaagccct tgcgattgat gatctcgatt tcgcgcacga gcgtgccgtc atcggcggcg
       61 gggacaggtt gcgatgtatc ggtcatttcc cgccgagcac ccgacttgcg acgtagatat
      121 atttgcggcc ggcgtcctgc gcctgcaaca cggcttgttc caggctcgtc gtatcgcgga
      181 tcgaagcgag cttgatcagc atcggcagat tgatcccggc gacaacttcg acctggccgc
      241 cattcatgac cgaaatggcg agattggatg gcgtgccgcc gaacatatcg gtgagaacga
      301 caacgccaat gccgcgatcc acctgcttca cagccgcgag gatatccttg cgtcgttctt
      361 ccatatcgtc ttcgggacca atggagacgg tggcgatctg cttctgcggg ccgaccacat
      421 gctcgagcgc cgcgcggaat tcggtggcca gatggccgtg ggtgacgagc accatgccga
      481 tcatcgacag caacgcctcc ggccgcgatg gcgcgataag acgcgcagga cggatggcga
      541 aacgccgaga ctgccgttca tttcttgctc tttgatctcg ggcgttgaag acggtcgcaa
      601 attcaagtcc aggcttcgag gcgctttttt ccggacgcaa ttggggacgc catcttgtgc
      661 agcgcggcga aattcgcaag aggaatgacg ccctcctgcg tcagattgcc ccaaattgtc
      721 gaagccgggc taaaacggta cgcgccgaat cggacgcgga accgatcttc gccaaagcca
      781 atagcggcaa gtctacgtcg caaagcgtaa cagaagggtt gatcgcctcg ggatagcgaa
      841 gcatctgttc cggtgggaaa atgtcgacga caagccggac gaccgcggcc ggctctgcgc
      901 cgatttccag aatcccgacg ccgcgctgct cgatctggcc gcgaatggcc ggatggccgc
      961 gctcaatcag ccggccatag ctcgcggaga gaagcagccg gtcgtcgccg atcaacgcgc
     1021 cgaaaagcgc tgcctgcgcc gctgcatcca ggagggccag cgcgagactg cttttgccga
     1081 tgcgagagac gccacggatt acgatccccg cctcgccgac cacgacgccg ctggcgtgga
     1141 tcgacacggg cgcgatcgct tctgcgctca ccgcgccgcc cggatccaga caatgaagcg
     1201 cgcgcccaag acgatgggac ttccgtccct gcccgtggtg atccggttca tcgcgcggat
     1261 accgccgcca tgcgcctcga tgatctggcg cgaaatcgaa agcccaagtc ccgaattctg
     1321 gccgaagccg tgattgggcc gatcggtata aaaacgctcg aagatgcgct cgaaggcgtc
     1381 ggcgggaatg cccgggccat cgtcatcaac aacgatctcg aaaccgtccg tcaacaaccc
     1441 attggtgagc gcgctctttg ccgggcgcag gccgacgcgc acgctcccgc cgggctgcga
     1501 aaatgacttc gcattgtcga taaggttgtt gaacacttgg cccagacgcg aatcatggcc
     1561 aaggacgatg aagccgttct ttttcgcgtc ttcgttgttg gcgaggtcga gcgtcacctg
     1621 cacactttca tgttccacat gattggccat cgtcaccaca gtgaggatga tacgcgagag
     1681 gtcgaccggc tccatgtcgg cgcgtgccag ttcggcatcg agtcgcgagg catcggaaat
     1741 atcgctgatc aatcggtcga gccggcgcac gtcatgctga atgatggcga gaagccgcgc
     1801 gcgtgaatca tccgtgcggg cgatcggcaa ggtctcgacg gcgctacgca aggaggtcag
     1861 ggggttctta agttcgtggg cgacatcggc ggcgaaactt tcgatcgcgt ccatgcgatg
     1921 atagagcgcc cgcgtcatgt cgcgcagcgc accggacaaa tggccgatct cgtcgctgcg
     1981 attggtgaaa tctggaattt cctggcgtga tttgatgccg cggcggaccc gctcggccgc
     2041 ttccgccagc cgatgcatcg gcccggcaat cgtgcccgcg agcaataggg acaagagcag
     2101 cataatcgcg gccgaaacca ggaagacccg gatgatggcc cagcgttccg aggcgatgat
     2161 cttatcgatg tcgccgcctt gcgtggacag cagcagcgcg ccgcggacgg tgcgcaaaag
     2221 ctcaatcggc accgcaacgg acacgatcgt ctcgccctta ttattcatcc gcacgatcga
     2281 ctgcgcctgg ccatcgagcg cgcgtgagac ttcgggataa gtccggccgt tggcgatgcc
     2341 gatgtcctcg tagagcggca attcgttata ggtgaacgat cgcttcatcg ccgcccagac
     2401 acgttcgagc cagttccccg cctcgtcgct ctgcgttggc aaatcgagcc gaaggatgtc
     2461 gccacgggcg ctcagcgcgc gggaatcgag cagcagataa ccatcgcggt cgtaaatgcg
     2521 cgcccgcgtg cgcgttggcg aaacaaggcg gcgcagcacg g
//
LOCUS       DASWOG010000648         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_254524,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000648 DASWOG010000000
VERSION     DASWOG010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254524"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGB93_13295"
     CDS             complement(<1..443)
                     /locus_tag="VGB93_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0147091.1"
                     /translation="MTKLENIRNFSIVAHIDHGKSTLADRLIQTTGTVAARDMVEQVL
                     DSMDIERERGITIKAQTVRLQYNAKDGETYILNLMDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYHALDAGHEIVPVLNKIDLPAAEPERIK"
     gene            666..1370
                     /locus_tag="VGB93_13300"
     CDS             666..1370
                     /locus_tag="VGB93_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0147092.1"
                     /translation="MQPTRLSRDDRRQQILECALPLFARHGFGGTTTRRIADAAGISE
                     ALLFKHFPTKSAIYAAILADICTADPGLARLRAEPPSTRTLALIVRGMTHYFISAADG
                     SDAEGNQKLRLTFSSYLEDGEFAKILFDKVEQVIAPIFINSLDHAIAVGDATRGGANS
                     RNLFWFAHHTLCTLAATQMPLLPSLDYPPPVELEREVCIYILRGIGLTDDAIEMLAAL
                     DIPFESFEASVLEFAR"
     gene            1367..>2061
                     /locus_tag="VGB93_13305"
     CDS             1367..>2061
                     /locus_tag="VGB93_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0147093.1"
                     /translation="MTLSAETEVSARTAYRKPKTDKPVRTKLWFSIMVVLMALLVGVF
                     WGFNQFRNHMIAQFFANMKMPPATVSVADVKSEVLPNLLTGIGDVVAVHQVNVTSEVS
                     GRVVKIMFEPGTHVKKGQPLVQLFDEPEQGDLASFQAQAVGAKLSLDRAKALALRQFG
                     PQATVDDQQATYDQAMAGISKTKAIISQKLILAPFDGELGLRHIEVGQFLNAGTQIVT
                     LTDLSVIWVNFTVT"
BASE COUNT          383 a          629 c          619 g          430 t
ORIGIN      
        1 tgcttgatgc gctcgggctc cgccgccggc agatcgatct tgttcagaac cgggacgatc
       61 tcgtgcccgg cgtcgagcgc gtgatagacg ttggcgagcg tctgtgcctc gacgccctgg
      121 ctcgcatcga cgacgagcag cgagccttcg caggccgcca gcgagcgcga gacttcgtag
      181 gcgaaatcga cgtggccggg cgtgtccatc agattgagaa tgtaggtctc gccatccttg
      241 gcattgtatt ggaggcgcac cgtctgtgcc ttgatggtga tgccgcgctc gcgctcgata
      301 tccatcgagt ccaggacttg ttccaccata tcccgtgcgg ccaccgtgcc ggtcgtctgg
      361 atcagccggt ccgccagggt cgatttgccg tgatcgatat gcgcgacgat ggagaaattg
      421 cggatatttt cgagcttggt catgcgtcgc gggatagcag gcgccccacg cgaaagaaag
      481 gcgtgcgttg cacgatcgcg gggattgtcc cggcaacatt ttggggccat cccgccgggc
      541 cgcccgcgct ttcattgccc ggagcgtcgg gcaccgggaa aattcggttc gcgccgcggc
      601 cgcggagggg agaaaaatct acggctcagc ttgacagatc ggtaagtgag tggtcactta
      661 cgaccatgca accgacccgg ctttcgcgcg acgatcgtcg ccagcagatc ctcgagtgcg
      721 cactgccgct cttcgcccgt cacggctttg gcgggacgac cacccggcgg attgccgatg
      781 ccgcggggat ttcggaagcg ctgcttttca agcacttccc gactaaatcg gcgatctatg
      841 cagctattct ggcggacatc tgtaccgccg atccaggtct tgcccggctg cgggccgagc
      901 cgccctcgac gcgcacgctg gcgctgatcg tgcgtggcat gacgcattat ttcatctccg
      961 ctgcggatgg ttcggatgcc gaaggcaatc agaagctgcg gctgaccttc tcgagctatc
     1021 tcgaggatgg tgaattcgcc aaaatattgt tcgacaaggt cgaacaggtc atcgcgccga
     1081 ttttcatcaa ttcgctcgat catgccatcg cagtcggcga cgccacgcgc ggcggcgcca
     1141 attcgcgcaa tctcttctgg tttgcgcatc acacgctctg cacgctggcg gcgacgcaaa
     1201 tgccgctgct gccctcgctc gactatccgc cgcctgtcga gctggaacgc gaagtctgca
     1261 tctacatcct gcgcgggatc ggcctcaccg atgacgcgat tgagatgctt gccgctctcg
     1321 atatcccctt tgaaagtttc gaagcctccg tattggaatt tgccagatga cgctctctgc
     1381 cgaaaccgaa gtctccgcca gaacggccta tcgtaagccc aagaccgaca agccggtgcg
     1441 caccaagctc tggttctcga tcatggtggt gctgatggcg ctgctcgtcg gggtgttctg
     1501 gggcttcaac cagttccgca atcatatgat tgcgcaattc ttcgcgaata tgaaaatgcc
     1561 accggcgacg gtcagtgtcg cggatgtcaa aagcgaggtt ctgcccaatc tcctgaccgg
     1621 catcggcgat gtcgtcgcgg tgcatcaggt caatgtgacg agcgaagttt ccggccgcgt
     1681 cgtgaaaatc atgttcgagc ccggcacgca tgtgaaaaaa ggccagccgc tcgtccaatt
     1741 gtttgacgag ccggaacagg gtgatctcgc cagcttccag gcgcaggcag tgggcgcaaa
     1801 actctcgctc gaccgcgcca aggcactcgc gttgcgccag ttcggcccgc aagcgacagt
     1861 tgacgatcag caggcgactt acgatcaggc catggcgggc atctccaaga ccaaggcgat
     1921 catttcgcag aagctgatcc ttgcgccgtt cgacggcgag ctcggcttgc gtcacatcga
     1981 agttggtcag ttcctcaatg ctggcacgca gatcgtcacc ctcaccgatc tgtcggtaat
     2041 ctgggtgaat ttcaccgtga c
//
LOCUS       DASWOG010000649         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_273453,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000649 DASWOG010000000
VERSION     DASWOG010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273453"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..914
                     /locus_tag="VGB93_13310"
     CDS             195..914
                     /locus_tag="VGB93_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0147094.1"
                     /translation="MPDADTEDFQEAGRKLFAQPCDFIWAATDAAQLPPPGTPEIAFA
                     GRSNVGKSSLLNALTRRNALARTSNTPGRTQALNFFALGELPKDSNNLKRDSREKPVS
                     TFSHPALLRLVDMPGYGYAAVGKAKVAAWTGMMHDYLRGRADLMRVFVLIDGRHGLKP
                     IDNEMFDQLDKAAVSYQIILTKADEVKISEQEPRLAATVAALAKRPAAFPEIILTSAK
                     TGAGIGDLRAAIAKLLAEHSS"
     gene            1038..>1711
                     /gene="argB"
                     /locus_tag="VGB93_13315"
     CDS             1038..>1711
                     /gene="argB"
                     /locus_tag="VGB93_13315"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0147095.1"
                     /translation="MSESAQESAQRQAEILMQALPHMLHYDDAIVVVKYGGHAMGDES
                     VARSFARDMVLLEQSGVNPVVVHGGGPQIGAMLKKLGIKSEFSAGLRITDRATVEIVE
                     MVLAGSINKQIVGFINAQGGRAIGLSGKDGNMVTARKFVHTVESPFTGEPDEVDLGFV
                     GEPSKVDTTVLDQVLGRELIPVLAPIAQGSEGETYNVNADTFAGAIAGALGAKRLLFL
                     TDVPGVL"
BASE COUNT          360 a          536 c          491 g          324 t
ORIGIN      
        1 gagttgtggg acaatctcgc caatatattc cgcaagaagg cgacgacctg atcgttcacg
       61 caacgtcatt gcgaggcgcc atcgctggcg atgtttcgca acagcgacat cgcaagtgcg
      121 ccgaagcaat ccagaaagta aaggctggat ggcttcgctt cactcgcaat gacatgcaac
      181 cgcaaagtgt agccatgccc gacgccgaca ccgaagattt tcaggaggcc gggcgcaagc
      241 ttttcgcgca accttgcgat tttatctggg ccgcaaccga cgccgcgcaa ttgccaccgc
      301 ccggcacgcc cgaaatcgca ttcgccggtc gctccaatgt cggcaaatcg tcgctgctca
      361 atgcgctgac ccgccgcaat gccctggctc gcacgtcgaa cacgccgggg cgcacgcaag
      421 ccctgaattt cttcgcgctc ggtgagctgc caaaagattc taataattta aagcgggatt
      481 cgcgcgagaa accggtctcc actttttcgc atcccgcttt gctgcgcctc gtcgatatgc
      541 cgggctatgg ctatgcggcg gtgggcaaag ccaaggtcgc cgcctggacc ggaatgatgc
      601 acgattatct gcgcggccgt gccgatctga tgcgcgtctt cgtcctgatt gacggacgtc
      661 acggtctaaa gccgatcgac aatgagatgt ttgaccaact cgacaaggcc gccgtctcct
      721 atcaaatcat cctgaccaag gcggacgagg ttaaaatctc cgagcaggag ccgcggcttg
      781 ccgccacagt tgcagccctt gccaagcggc cagccgcctt tcccgagatt atcctgacct
      841 cggccaagac cggcgccgga attggcgact tgcgcgcggc gatcgcgaaa ctgctggccg
      901 aacattcatc gtaaggtcac gtctcccggt ctaagcgacg gtttctgatg atttatcgga
      961 tgagcgcgga aaaaagcgtc gcactttccc tcgcttagct ctgacgttaa aaggccgcac
     1021 caacccggat gagcacgatg tcagaatcag cgcaggaaag cgcacagcgg caagccgaaa
     1081 tcctgatgca ggcgctgccg catatgctgc attacgacga cgcgatcgtc gtggtgaaat
     1141 acggcggcca tgcgatgggc gacgagagtg tggcccgcag cttcgcccgc gatatggttc
     1201 tgctcgaaca atccggtgtc aatccggtcg tcgtccacgg cggcggcccg cagatcggcg
     1261 caatgctgaa aaaactcggc atcaaatcgg aattttccgc cggcctgcgc atcaccgacc
     1321 gcgccaccgt cgagatcgtc gaaatggtgc tcgccggttc gatcaacaag cagatcgtcg
     1381 gtttcatcaa tgcccaaggc ggccgcgcca ttggcctctc cggcaaagat ggcaacatgg
     1441 tcacggcgcg gaaattcgtc cacacggttg aatcgccctt taccggcgag ccggatgaag
     1501 tcgatctcgg cttcgtcggc gagccgagca aggtcgacac caccgttctt gatcaggtgc
     1561 tcggtcgcga attgatcccg gtgctcgcgc cgatcgcaca aggcagcgag ggcgagactt
     1621 acaacgtcaa tgccgacacg ttcgccggcg ccatcgccgg cgcgctcggc gccaagcgct
     1681 tgctcttctt gacggacgtg cccggcgtgc t
//
LOCUS       DASWOG010000650         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_125906,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000650 DASWOG010000000
VERSION     DASWOG010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125906"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VGB93_13320"
     CDS             complement(<1..996)
                     /locus_tag="VGB93_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEY0147096.1"
                     /translation="MTAQPTARIQPAAKAGSTRTTMDGNTAVAHVAYRLNEICAIYPI
                     TPSSTMAELADQWSADGVCNIWGNIPMVQEMESEGGAAGAVHGALQTGALTTTFTASQ
                     GLMLMLPNMMKIAGELTSAVFHVAARSVATSALSIFGDHADVMTIRPTGFGIVSSAAV
                     QDAHDMALITQIATLEARVPFVHFFDGFRTSHELNTLDVLTDAQLRSLIDDDLVRAHR
                     ARALDPEHPFIRGTAHNPDTFFQTREAVNAYYDRVPGILQDAMNRFGAMTGRNYRLFE
                     YDGDPEAEHVVIVMGSGAETVRTTMQHLRAAGEKVGMVQVRLFHPFSPAHLLAALP"
     gene            complement(1067..2695)
                     /locus_tag="VGB93_13325"
     CDS             complement(1067..2695)
                     /locus_tag="VGB93_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0147097.1"
                     /translation="MSAIAHDLTPDVDLGLRRVMGPVRTAHPIYVRHLPPCNKACPAG
                     EDIQGWLDLAQAGRYQEAWAHILRDNPMPGVHGRVCYHPCETGCNRGAVDEPVGIHAV
                     ERFLGDRAMAEGWAPPQAAAASGKRVLIVGAGPAGLSAAYHLALAGHEVEIREAGAAP
                     GGMLRFGIPAYRLPRDVLEHEVARITALGVRLILDHKVSDVLAEKAAGGFDAVFVAIG
                     AEAPRRVEIPVRDAAHVVNAVDLLGAVGRGGMPKLGRRVFVYGAGDTAMDAARTALRL
                     GAAETAIVFFSDRAHMEAHPFEAREAAQEGITFNWLSSVRDVDGEHVRIELMELDDKG
                     VAHPTGRFKTLTADSVVLALGQTAESGFLAKLPGVTLNPDGTVQVGADLTTGAPGIFA
                     GGDLVPGPRTVTQAVGDGKKAARNIDGYLRGKPYVHPPEHPGVLYTDLNLSIYTDAPQ
                     VHQPEVSADQRRDFAEFTGGLTAAQAVREAKRCFSCGNCYECDNCYAACPEQAISRRG
                     VGKGYSVDLAKCTGCGACFEQCPCHAIEMEPEPA"
BASE COUNT          619 a          979 c          937 g          576 t
ORIGIN      
        1 cggcagcgcc gccaagagat gcgctgggga gaacggatgg aaaagccgca cctgcaccat
       61 gccgaccttc tcgccggcgg cgcgcaggtg ctgcatcgtc gtgcgcacgg tttcggcacc
      121 cgaacccatg acgatcacca catgctcggc ctcgggatcg ccatcatatt cgaacaatcg
      181 gtagttgcgg ccggtcatcg cgccgaatct gttcatggca tcctggagga ttcccggcac
      241 ccgatcataa taggcgttca ccgcctctcg cgtctgaaag aacgtgtcgg gattatgggc
      301 tgtgccgcgg atgaagggat gttccgggtc gagcgccctc gcccgatgcg cgcgcaccag
      361 atcgtcgtcg atcagcgaac gcaactgcgc gtcggtgagc acatcgagcg tgttcaactc
      421 gtgtgaggtg cggaaaccgt caaagaaatg gacgaacggt actcgcgctt cgagcgtcgc
      481 gatctgcgtg atcagcgcca tgtcatgcgc gtcttgcact gcagcggagc tgacgatccc
      541 gaagccggta gggcggatcg tcatgacgtc ggcgtgatcg ccgaagatcg acagcgccga
      601 agtggcgacc gagcgcgccg ccacatgaaa caccgccgaa gtaagttcgc cggcgatctt
      661 catcatgttg ggcagcatta acatcagtcc ctgcgaggcg gtaaaggtcg tggtcagcgc
      721 ccccgtctgc agtgcgccat gcaccgcgcc agcggcgccg ccctcgcttt ccatctcctg
      781 caccatcggg atattgcccc agatgttgca cacgccatcc gccgaccact ggtcggcgag
      841 ctcagccatc gtggaagacg gcgtgatcgg atagatcgcg cagatttcat tgagtcggta
      901 ggcgacatgc gcgacagcgg tgttgccgtc catcgtggtt cgtgtcgaac cggctttggc
      961 cgcgggctga attctggccg tgggttgggc ggtcatcctg ggatcatcct tatcggggcg
     1021 cacaagccca cgaaatcaaa aagactcgcg agcggaaatt ggcggatcag gcgggctccg
     1081 gctccatctc gatagcgtgg cagggacatt gctcgaaaca ggcgccgcag ccggtgcatt
     1141 tggcgagatc gacgctatag cctttcccga cgccgcgccg actgattgcc tgttctggac
     1201 aggcggcata gcaattgtcg cattcatagc aattgccaca ggagaaacag cgtttggctt
     1261 cgcgcaccgc ctgcgccgcg gtcaagccac cagtgaattc ggcgaagtcg cgccgttgat
     1321 cggcggagac ttccggctgg tgcacctgcg gcgcgtccgt gtagattgac aggttcaggt
     1381 cggtataaag cacaccggga tgttccggcg gatgcacata aggcttgccg cgcagatagc
     1441 catcaatgtt gcgcgccgcc tttttgccgt cgccaacggc ctgggtgacg gtgcgcggac
     1501 cgggcaccag atcgccgccc gcaaatattc ccggcgcgcc ggtcgtcaaa tccgcgccga
     1561 cttgcaccgt accgtccggg ttgagggtca ctcccggcag cttggcgaga aagccgcttt
     1621 cggccgtttg cccgagcgcg agcacaacgg aatcggcggt cagcgtcttg aagcggccgg
     1681 tcggatgcgc aacaccctta tcgtcgagtt ccatgagctc gatgcgcaca tgttcaccat
     1741 cgacgtcgcg cacgctgctc agccagttga atgtgatgcc ttcttgcgcg gcctcgcgcg
     1801 cctcgaacgg atgggcttcc atatgcgcgc ggtcggagaa gaagacgatg gccgtctccg
     1861 cggcacccaa ccgcagcgcg gtgcgggcgg cgtccatcgc ggtgtcgccc gcgccgtaga
     1921 caaatacgcg gcgtccaagc ttcggcatcc cgccgcgacc cactgcgccc aataaatcca
     1981 ccgcattgac gacatgggcc gcgtcgcgca ccggaatctc aacgcgccgc ggcgcttccg
     2041 ccccgatcgc gacaaagacc gcgtcgaaac cgccagcggc tttctcggcc agtacgtcgc
     2101 tcactttgtg atcgaggatg aggcgaacgc ccaaggccgt gatacgggca acttcgtgtt
     2161 cgagcacatc gcgcggcagg cgataggcgg gaatgccgaa gcgcagcatg ccgcccggcg
     2221 cggcacccgc ctcacggatt tcaacttcgt ggccggccag cgcgagatga taggccgccg
     2281 ataatccggc cggcccggcg ccgacgatga gcacccgctt gccgctcgcc gccgccgctt
     2341 gcggcggtgc ccaaccttcg gccatcgccc gatcgccgag gaaacgctcg acggcatgaa
     2401 tgccaaccgg ctcatctacc gcaccacggt tacagccggt ctcacaagga tgatagcaaa
     2461 cccggccatg cacgccgggc atcggattgt cgcgcaggat atgtgcccag gcctcctggt
     2521 agcgccctgc ctgcgccaga tcgagccagc cctggatatc ttcgccagcc ggacaagctt
     2581 tgttgcaagg cggcagatga cgcacataaa tcggatgtgc cgtccgtact ggccccatta
     2641 cgcgccgcaa gccaagatcg acatcgggcg tcaagtcatg cgcaatcgcg ctcattgcgc
     2701 catccttttc atctcttgtc gacgggttct gccgacgcgc cgattacccg tctatttggc
     2761 tccttatgaa aggggccgac tgcaaacatt gacgtaaatc aagatcatgg cgaacaagtt
     2821 agaatgaaaa taattctatc ggcgcatccc aacaattaat ttgatgatat catttgaaag
     2881 atgcgcttct ctcggcgaag aattatgagt gtgagcgcag aatcattttg gaatactttg
     2941 atcttaaagc acatcgtgca cgaacttgat ccatgcatca cacgcttgag atttgagaac
     3001 catggcctga gaaggtgtgc tcaatcgcta catgaaacga tccgcgccgc cggaagcctc
     3061 gatcgtgtcc ccggtgacga aaccgttacg ctccgaggct aggaaggcga c
//
LOCUS       DASWOG010000651         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_52167,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000651 DASWOG010000000
VERSION     DASWOG010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52167"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..554
                     /locus_tag="VGB93_13330"
     CDS             48..554
                     /locus_tag="VGB93_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147098.1"
                     /translation="MRKGWASYIFAGALAFAFGSTGAEAASKHHHHAQTHYSRYQNVG
                     GMSVGVMEPGKVYSKYGHYTELAGDPHSGLGFYPLPYQYRVGAWRYHMRHAPPIPPVI
                     QAARDQAVESYSWEMPTPDDAYAYGVYNPVDGVGSPFFAGYYGPAGDDEDDDSDSGFP
                     FGHSYTPR"
     gene            complement(551..1144)
                     /locus_tag="VGB93_13335"
     CDS             complement(551..1144)
                     /locus_tag="VGB93_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0147099.1"
                     /translation="MPSDLRHVTRVLIGWNAGMFAYFIAVGAMVWSSDNRSLARRAAA
                     QDDGRFAILFLTIFAAAAAFGAIFYQLLLVKDEHGLNRSFHLGLAALTVISSWAFIHV
                     MFALHYASECFFVRNTKVSPEAKVHGGLRFPGEDEPPDYLDFFYFSFVIGVACATADV
                     NITSRSIRRTALIHSVVAFFFNSAVLALTVNIAAGLV"
     gene            complement(1255..2181)
                     /locus_tag="VGB93_13340"
     CDS             complement(1255..2181)
                     /locus_tag="VGB93_13340"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0147100.1"
                     /translation="MTQSAEEEFHARLSSVADATEHMLDRLLSETPLPGENARPSRFL
                     GAVRYACLNGGKRLRPFLLVETARLFGIESEGVLRAGAAVEMIHCYSLVHDDLPALDN
                     DDLRRGRPTTHKAYDEATAILVGDALLTYAFDVTADPATDPDPAIRAALVLALARAAG
                     IGNMVGGQVMDLEAETSKEPHTAEHVIQVQVMKTGALLHYSVEAGGIFARADEKARTA
                     LSRYGKALGAAFQVADDILDVEADEAALGKRAGKDADRNKATLVAALGLDAARKRRDA
                     LAQEAISALDVFPAGRAAVLKEAARFAVARKN"
     gene            2313..>2867
                     /locus_tag="VGB93_13345"
     CDS             2313..>2867
                     /locus_tag="VGB93_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0147101.1"
                     /translation="MARNYRLSHVFSTAFRWGVRIALLLFLLLVGLIVAYRFVDPIST
                     IMLGRMIAQEKVTQKFVPLNRISENLRVAVVTSEDAHFCHHHGVDWGAMREVINESGI
                     DGPSRGASTITMQVAKNLFLWPGHSYIRKAFEIPLALVLDFVWPKRRILEIYLNIAEW
                     GDDGIFGAEAASNAYFHEPAADLTL"
BASE COUNT          582 a          911 c          844 g          530 t
ORIGIN      
        1 ttgtcgcgag atgcggcgag atttgaccgc tgcatggaga ttgaccgatg cgcaagggct
       61 gggcttctta tattttcgcg ggcgcgctcg cgttcgcctt cggctcgacc ggcgctgagg
      121 ctgcgagcaa acatcatcac catgcgcaga cgcattacag ccgttatcag aacgttggcg
      181 gcatgtcggt cggcgtgatg gagcccggca aggtctattc caaatacggc cactataccg
      241 aactcgcggg cgacccgcac agcggcctcg gcttctaccc gctgccttat caataccgcg
      301 tcggcgcctg gcgttatcat atgcgccacg ccccgccgat cccgccggtg atccaggcgg
      361 ctcgcgacca agcggtcgaa agctacagct gggaaatgcc gacgcccgac gacgcctatg
      421 cttatggcgt ctataatccg gtcgatggtg tcggttcgcc gttcttcgcg ggctattatg
      481 gcccggccgg cgacgatgag gacgacgaca gcgattcagg cttcccgttc ggtcattcct
      541 acacgccgcg ctagaccaag cccgcggcga tattgaccgt cagcgccagc acagcgctgt
      601 tgaagaaaaa ggccacgacg ctgtgaatga gcgccgtgcg ccggatcgag cgtgacgtaa
      661 tattgacgtc agccgtggcg caggcgacgc cgatgacgaa agagaaataa aagaaatcga
      721 gataatccgg cggctcgtcc tcgccgggga agcgtaggcc accatggact tttgcttcag
      781 gtgacacctt cgtattgcgc acaaaaaaac attcgctcgc gtaatgcagc gcgaacatca
      841 cgtggatgaa cgcccaggag ctgatgacgg tcagcgcggc aagcccgaga tggaacgaac
      901 ggttcagacc atgctcgtcc ttcaccagca ataattgata gaagatcgcg ccgaaggcag
      961 ccgccgcggc gaagatcgtc aggaaaagaa tagcgaaccg gccgtcgtcc tgcgcggccg
     1021 cgcggcgagc caaagaacga ttgtcgctcg accacaccat cgcgccaact gcgataaaat
     1081 aggcgaacat tccggcattc caaccgatca gcacgcgcgt gacatggcgc aaatcgcttg
     1141 gcattgccaa cccgacaagg gcgccaagcg ccaggcagac gaacaggcgc ggccgcgacc
     1201 ggataacccg aacgggccag ccgaaacgcc ggcgacgcga gaggcctgtc ccggtcaatt
     1261 tttacgcgcg acggcgaaac gcgccgcttc tttcaagacc gcggctcgtc ccgcagggaa
     1321 gacatcgagt gcgctgatgg cctcctgtgc cagagcatcg cggcgcttac gcgccgcatc
     1381 aaggccgagc gcagcaacaa gcgtcgcctt gttgcgatcg gcgtctttgc cggcgcgttt
     1441 gccgagggcc gcttcgtcgg cctcgacatc gagaatatcg tcggcgacct gaaaggccgc
     1501 tcccagcgcc ttgccataac gcgacagtgc cgtgcgcgcc ttttcgtcgg cgcgggcgaa
     1561 aattccgccg gcttcgaccg aataatgcaa cagtgcgccg gtcttcatca cctgaacctg
     1621 aatgacatgc tccgccgtgt gcggctcttt cgacgtctcg gcttcaaggt ccatcacctg
     1681 gccgccgacc atgttgccaa tgccggcggc gcgcgcgagc gccaggacca gcgcggcgcg
     1741 gatcgccggg tcgggatcgg tcgcggggtc cgccgtcacg tcgaaagcgt aagtcagaag
     1801 cgcatccccg acgagaatag cggtcgcctc atcataagct ttgtgtgtcg tcggacggcc
     1861 gcggcgcaga tcgtcattat cgagcgctgg cagatcatcg tgcaccagcg agtagcaatg
     1921 gatcatttcc accgccgcgc cagcgcgcag gacaccctcg ctctcgatgc cgaaaagtcg
     1981 cgcggtctcg acgagcagaa atgggcgcag ccgtttgccg ccgttgaggc atgcatagcg
     2041 cacggcgcca agaaaccgcg acggacgagc gttttcgccg gggagcggcg tctccgacaa
     2101 gagacggtca agcatgtgct ccgtggcatc cgccaccgag cttagccgcg cgtgaaattc
     2161 ttcttccgcc gattgcgtca taatatccct gccacgaacg ctttagaccg cgtgttctcg
     2221 tcaaaaaagt ctgcaacttt ttcggaacac gcttttaaac caaatccggc gttccgcttg
     2281 cccgatcgcg gaagatcggt caaggctcgg aaatggcccg caattatcgg ctttcccacg
     2341 tattcagcac ggcgttccgc tggggcgtcc ggatcgctct gctgcttttc ctgctgctcg
     2401 tcggcctcat cgtcgcttat cgcttcgtcg atccgatctc gaccatcatg ctcggtcgca
     2461 tgatcgcgca agaaaaagtc acgcagaaat tcgtgccgct caaccgcatc tccgagaatt
     2521 tacgcgtcgc ggttgtcact tcggaagacg cgcatttctg ccaccaccat ggcgtcgatt
     2581 ggggcgcgat gcgggaagtg atcaatgaaa gcggcatcga cggcccgtcg cgcggcgcct
     2641 cgacgatcac catgcaggtc gccaagaacc tctttctctg gccgggccat tcttatatcc
     2701 gcaaggcctt cgaaattccg ctcgcactcg tcctcgattt cgtctggccg aagcgccgca
     2761 tcctcgaaat ctacctgaac atcgccgaat ggggcgacga tggcattttc ggcgccgagg
     2821 cggcgtccaa cgcctatttc catgaaccgg ctgccgatct gacgttg
//
LOCUS       DASWOG010000652         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_254639,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000652 DASWOG010000000
VERSION     DASWOG010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254639"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1209
                     /gene="dinB"
                     /locus_tag="VGB93_13350"
     CDS             88..1209
                     /gene="dinB"
                     /locus_tag="VGB93_13350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0147102.1"
                     /translation="MTTGPFSEDDGRGRPRAEPRKVIHIDMDAFYASVEQRDNSDLRG
                     KPVAVGGANRRGVVAAASYEARKFGVHSAMASITAVRKCPELIFVKPRFDAYRAISQQ
                     IRAIFAEYTDLIEPLSLDEAYLDVTQNKLAAPLATQIAEDIRAKIRAETRLTASAGVS
                     YNKFLAKLASDYRKPDGLFVITPKMGPAFVESLAVGRFHGVGPATAAKMKGLGIETGA
                     DLKACALQFLEDHFGKAGAYYYWVARGIDERPVRPDRTRKSIGAENTFFDDLIEFEAM
                     RDALLPILNKLWRHCEGGGIRGRTVTLKMKFSDFRIITRSRSISGCVNSRDELEAISL
                     DLLRHLLPPVLGVRLLGVSVSNLVSSEGDGGAAGQMILL"
     gene            1364..1747
                     /locus_tag="VGB93_13355"
     CDS             1364..1747
                     /locus_tag="VGB93_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0147103.1"
                     /translation="MAVAKKKTKAAPAVAARATPSTKAVARGAAVTRTAVKTVTLKQI
                     GHELAEAYELPKRTVESLFNDMTDTIIKNIKKGSKVRISGIGILQVKKRAARMGRNPA
                     TGESIKIKASKKVALSVARDLKQSL"
     gene            complement(1936..>2384)
                     /locus_tag="VGB93_13360"
     CDS             complement(1936..>2384)
                     /locus_tag="VGB93_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147104.1"
                     /translation="SVNRAIAMLRLDNFFTTIKAMVELDDGSVAKKSTVTVEINPITF
                     GERESKMIRELQARHDACHRVAHEGELEIIVPIIGVKPLCLRREEMLGRCFATPPVIC
                     SDHFLDARSSGSLDMNKNAPIFMADHRSGVPPFKSQNATENRNHHN"
BASE COUNT          507 a          661 c          691 g          525 t
ORIGIN      
        1 gaagctcccg agattggact gtaccgccgc gaacgctatg atccgtaaga ttccccgagc
       61 acgcggcgcg cggaggttgc gtattcgttg acgaccggtc cattttctga agacgatggg
      121 cgaggccggc cacgcgccga acctcgcaag gtgattcaca tcgacatgga cgcgttttac
      181 gcgtcggtcg aacagcgcga taattcggac ttgcgcggta aaccggtcgc ggtcggcggg
      241 gcgaatcgcc gcggcgtcgt tgccgcggct agctacgagg cgcgaaaatt tggcgtccac
      301 tcggcgatgg cgtccatcac ggccgtgcgg aaatgccctg agctgatttt cgtcaagccc
      361 cggtttgacg cctatcgcgc gatctcgcag cagatcaggg cgatttttgc ggaatatacc
      421 gacctgatcg agccgctttc gctcgacgaa gcctatctcg atgtcacgca gaacaagctc
      481 gccgcgccac tggccacgca gattgccgag gacatccggg ctaaaattcg cgccgaaacg
      541 cgtctcacgg cgtccgccgg cgtctcctac aacaagtttt tggcgaagct cgcttcagat
      601 tatcgcaagc ccgacgggct ctttgtcatc acgccgaaaa tggggcctgc gtttgtcgag
      661 tcgctcgccg tcgggcgctt ccatggcgtt ggtcccgcta ccgcggccaa aatgaaaggc
      721 ctcggcatcg aaaccggcgc cgacctcaaa gcctgcgcgc tgcaattcct tgaggatcat
      781 ttcggcaagg cgggagcgta ttattattgg gtcgcccggg gcatcgatga gcggccggtg
      841 cggcccgacc gcacccgcaa gtcgattggg gccgagaata ctttcttcga tgatcttata
      901 gaattcgagg cgatgcgcga cgccttgctg ccgatcctca acaagttgtg gcggcactgc
      961 gaggggggag gcatccgcgg gcgcaccgtg acgttgaaga tgaaattttc ggattttcgc
     1021 atcatcaccc gcagccgttc gatcagcggc tgcgtaaact cccgcgacga acttgaggcg
     1081 atcagcctgg atctgcttcg acacttgttg ccgcctgtct tgggcgttcg gctgctcggc
     1141 gtgtccgtct cgaaccttgt ttcgagcgag ggtgacggcg gcgccgcggg tcagatgatt
     1201 ttgctctgaa cgccggtctc ggacttcagc aagccttgca gcgctgcaaa cttccgccac
     1261 aaatgatgag agtttgcagc ggcgcaaggt acggcgcgca ttcgcatctg gcatatgcta
     1321 aaagctgcgc gctatgattc gtttcgagca aagaggtagc tctatggcag ttgcaaaaaa
     1381 gaagactaaa gcggcgcctg cggttgcggc gcgcgccaca cccagcacta aagccgttgc
     1441 ccgtggtgca gcggttacca ggacggcggt caagacggtg acgttgaagc agatcggcca
     1501 tgaactcgcc gaagcctacg agcttccgaa gcggacggtc gaatcattgt tcaacgacat
     1561 gaccgatacc atcatcaaga atatcaaaaa gggctcgaag gttcgcatca gcggcatcgg
     1621 catcttgcag gtcaagaagc gtgccgcacg tatggggcgg aacccggcga caggcgagag
     1681 catcaagatc aaggcgagca agaaagtggc gctcagcgtc gcccgcgacc tgaaacaatc
     1741 gctttaagcc catctcttga tgacagattg cgcgatcata tcgcgcaatc tgttagatca
     1801 cggcagcgta aatcgtgacg cggacggcca attttcgcgc cgcaataatg atttgcaccc
     1861 cacgttgctt gaacttcaag ctttacaaat tttggttaat cacagaaatg tcaatggatt
     1921 gatttttcca cgcgtttaat tgtggtggtt gcggttttct gtcgcgtttt gcgatttgaa
     1981 ggggggcact ccgctgcgat gatcagccat aaatatcggt gcatttttat tcatatccag
     2041 cgaaccgctg gatcgagcat cgaggaaatg atcactacag atgactggtg gcgtcgcgaa
     2101 gcatcgacca agcatctcct cgcgtctcag gcaaagcggc tttacgccga ttattgggac
     2161 gattatttca agttctcctt cgtgcgccac ccggtgacac gcgtcatgtc ttgcttgaag
     2221 ttcacggatc attttggatt ctcgctctcc aaaggtaatc ggattaattt cgacggttac
     2281 cgtggacttt ttggcgacag aaccgtcgtc gagttcgacc atcgctttta tcgtcgtgaa
     2341 gaaattatcc agccgcagca ttgcgatggc tcggtttacg gaaa
//
LOCUS       DASWOG010000653         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_200502,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000653 DASWOG010000000
VERSION     DASWOG010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200502"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..632)
                     /locus_tag="VGB93_13365"
     CDS             complement(183..632)
                     /locus_tag="VGB93_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0147105.1"
                     /translation="MSALAFDAPRPHVGVSISVFRDGRVLLATRTKPPYAGFFTLPGG
                     HLEAGEKVADAALREIEEETGVTAELVGFNQYVEIIAPPDADGLTRHFVILSFVARWI
                     SGEGTPGPEAGEILWTYPDEIAQLATSPHLIEVVTNAHKILMQKRPG"
     gene            complement(629..1243)
                     /gene="pdxH"
                     /locus_tag="VGB93_13370"
     CDS             complement(629..1243)
                     /gene="pdxH"
                     /locus_tag="VGB93_13370"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0147106.1"
                     /translation="MDKLTVCDFTEADEPFALFADWMAEAEKAEPNDPNAMALATTGA
                     DGLPDVRMVLLKSFDLNGFVFYSNSESAKGLELAQNMKAAVLFHWKSLRRQVRIRGLV
                     EKASEAEADAYFASRALQSRIGAWASQQSRPLESRFALQKAAAQYAAKFALGTVPRPS
                     YWIGYRIVPVAIEFWSDGAFRLHDRIVFTRATPQGGWHKQRLYP"
     gene            1358..1756
                     /locus_tag="VGB93_13375"
     CDS             1358..1756
                     /locus_tag="VGB93_13375"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEY0147107.1"
                     /translation="MLAVLFTAATLSGCSVSMPMGSLLGDDDSTGSIQKSQFDGLVGE
                     DWRRAKAALASALGGDEAKNSVAWANPDSGAKGSFVPVGSAYPTASGTCRAFAAAVDR
                     HDADDSLQGTACAGKDGDWLVTDVHAVGKG"
BASE COUNT          414 a          636 c          642 g          406 t
ORIGIN      
        1 ggcgcggacg gcgcgggatc gtccgcgggg gctcgaattc ctgcggccgg tattgcggcg
       61 gcgctccaaa aagattgaaa ccgccgccgc caccgccaaa ccagccttgc gcctgcgcgg
      121 ccagcggcgc aaaggtggaa agcgcactga cgaggacgat cacgtatcgt ttgaccacga
      181 gttcagcccg gacgtttctg catcaaaatc ttatgcgcat tggtcacgac ttcgatcagg
      241 tgtggcgatg tcgcaagttg cgcaatttca tccggataag tccacagaat ttcgcccgct
      301 tcagggccgg gggtgccttc gccggaaatc catcgcgcca cgaaggagag aatgacaaag
      361 tgccgggtca ggccgtcggc atccggcggg gcgatgattt cgacatattg attgaagccg
      421 acaagctcgg ccgtgacgcc ggtttcctct tcgatttcgc gcaaagcggc atcggcgacc
      481 ttttcgccag cctccagatg accgccgggc agggtgaaaa atcccgcata gggcggcttg
      541 gtccgtgttg cgagcagaac gcggccgtcg cgaaagacgg aaatgctcac gccgacatgc
      601 ggacgcggcg cgtcgaacgc caatgcgctc acgggtacag ccgctgctta tgccagccgc
      661 cttggggcgt tgcgcgggtg aagacgatgc gatcgtgaag ccggaacgcg ccatccgacc
      721 agaattcgat cgcaactggc acaatccgat agccaatcca ataggaaggc cgcggcactg
      781 tgccgagcgc aaatttggcg gcatattgtg cggccgcttt ttgcagcgcg aaacggcttt
      841 cgagcgggcg cgattgctgg ctggcccagg cgccgatgcg gctttgcaat gcgcggctgg
      901 cgaaataagc gtcggcttcc gcctcgctcg ccttttcgac gaggccacgg atgcgcacct
      961 gccggcgcag cgatttccaa tgaaacagca cagcggcttt catattctgc gccagttcga
     1021 gccctttggc gctttccgaa ttgctataaa aaacaaagcc gttaaggtcg aacgacttca
     1081 acaagaccat gcggacatcg ggcaggccat cggcgcccgt cgttgccagc gccatcgcat
     1141 ttgggtcgtt gggctcggcc ttctccgcct ctgccatcca atctgcaaag agcgcgaatg
     1201 gctcgtcagc ctcggtaaag tcacacacag ttaatttatc catgcctagg ctttcagtgc
     1261 cattgaatga ggttaagctt tgtccaacag gcgggggtcg tgttgcatgc gttatacggg
     1321 aaatagagtc ggtctgacga gggcttcagc gtggcctttg cttgccgtcc tgtttaccgc
     1381 cgcgacgctc agcggctgtt ccgtatcgat gccgatgggc tcgctgctcg gggatgacga
     1441 ttcgaccggc tcgatccaga aatcgcaatt cgatggtctc gttggcgaag attggcgccg
     1501 cgccaaggcg gcgctcgcct cagcgcttgg cggtgacgaa gccaaaaata gcgtggcgtg
     1561 ggcaaatccc gactccggcg ccaagggctc gtttgtgcct gtcggctccg cctacccgac
     1621 ggcgtccggc acctgccgcg ccttcgctgc ggcggtcgat cgtcatgatg ccgacgattc
     1681 gttgcaaggc acggcctgcg ccggcaagga cggcgattgg ctggtcaccg atgtccacgc
     1741 ggtcggtaaa ggctgagcac ccgggatttt cgggagcgtg cgcgtgaggc ttgcgccaaa
     1801 cgcatgaaac ccccaagttg gcggtgagac atttgcgccc gtgccatagc gcttgttccg
     1861 aaaaagttgc atgacttttt cgatcagaac atgctccaac gttatgaatt ggggcgattt
     1921 cttatcgacc agaggagtcc atctggccga aaagcccgct agacgctccg ccgccgcaag
     1981 gctcaatgga gctccctcct tcctccccag gacctgctcg caggaatgaa aaccagccga
     2041 agccaccgca acgccgcatt gagcaaagcc ccgatcgccg cgcggctggc aattccgc
//
LOCUS       DASWOG010000654         9481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_70876,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000654 DASWOG010000000
VERSION     DASWOG010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70876"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..320)
                     /locus_tag="VGB93_13380"
     CDS             complement(180..320)
                     /locus_tag="VGB93_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147108.1"
                     /translation="MPIRVLALAAIVTLSLFVSGCTSCGQFEKFNAPTMPKLCHADNS
                     PN"
     gene            504..902
                     /locus_tag="VGB93_13385"
     CDS             504..902
                     /locus_tag="VGB93_13385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0147109.1"
                     /translation="MQEHRTTERVRSFLRAQIVFNNRMTTIECIIKNYSAAGAKIALN
                     DTLTVPTEFDIYIPAKQRNHHARLVWRDKDAIGVNFIEAGAAVATKTPAAAAADRLRE
                     LELQNAELKSRVRELSKRLEDLGQDPNIAA"
     gene            complement(913..1596)
                     /locus_tag="VGB93_13390"
     CDS             complement(913..1596)
                     /locus_tag="VGB93_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0147110.1"
                     /translation="MSEPVLQLKGITRVYKQGEEALSVLADADFALFPGQSVALVAPS
                     GTGKSTLLHIAGLLERPDSGEVFINGVATTRLSDDSRTALRRNAIGFVYQFHHLLPEF
                     SAIENIMLPQMIAGLSRRKAEVRALELLDYLGLVARAKHRPTELSGGEQQRVAIARAV
                     ANAPGLLLADEPTGNLDPHTADHVFASLETLVQATGLATLVATHSQTLAAQMTRRVTL
                     DDGRVREME"
     gene            complement(1598..2854)
                     /locus_tag="VGB93_13395"
     CDS             complement(1598..2854)
                     /locus_tag="VGB93_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY0147111.1"
                     /translation="MAMFAPFEWTIATRYLRARRSSGFVSVIAGFSFLGITLGVATLI
                     VVMSVMNGFHRDLLNKIVGINGHIFLQAADAPLTDYNQVAKQVEKVPGVDLAIPMVEG
                     AAFGASDFAQNGSGILVRGIEEADIKHLPGVSGTIRSGTLNGFDANDGVAIGQRLAES
                     LNVRIGDKIKIMTANGARTPFGVAPRVKAYKIVAIFEIGMAEFDGIFVYMPLPEAQAF
                     FNLDKQVSVVELFLNDPENIDAIRDKIETTVTRPIIMTDWRERNKTFFDALNVERNVM
                     FIILTLIVLVASLNIISGLIMLVKDKSHDVAILRTMGATRGAIMRIFLITGASIGIVG
                     TLAGFLLGLLVAENVEPIRQFLNHVLHANLFPAELYFLSRLPSVVDPRDVATVVCMTL
                     ALSILATLYPSWRAAKLDPVEALRYE"
     gene            complement(2878..4197)
                     /locus_tag="VGB93_13400"
     CDS             complement(2878..4197)
                     /locus_tag="VGB93_13400"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0147112.1"
                     /translation="MRLSRYFLPILRENPKEAEIVSHRLMLRAGMVRQEAAGIYAWLP
                     LGFRVLENVTQIVREEMDRAGAIELLMPTLQLADLWRETGRYDAYGPEMLRIKDRHER
                     ELLYGPTNEDMITEIFRASVRSYRDLPKNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
                     YSFDIDEAAARRSYQRMFVTYLRIFARMGLKAIPMRAETGPIGGDQSHEFIILADTGE
                     SGVFINSDVLDLPIPTADVDYDSDLTPIIDQWTSLYAATDDVHDAARFERETPADKRL
                     QKRGIEVGQVFYFGDKYSKPMKAVVTGPDGVERPVLGGSYGVGVSRLVGALIEAGHDE
                     AGIIWPESVAPFRIGIANLKVGDAATDAASLKIYDALQKAGIDVLYDDTDERPGGKFA
                     RLDLIGLPYQIIIGPKGLAEGQVEVKTRSTGIRENLSLEAVISRFAG"
     gene            4427..5827
                     /locus_tag="VGB93_13405"
     CDS             4427..5827
                     /locus_tag="VGB93_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEY0147113.1"
                     /translation="MSAAEHLDVLVLGSGQAGNLMAWHMARSGYRTALIERRWIGGSC
                     PNFACMPSKNEVWSARVAHLVRNAGHFGTITTPGKIDMGQVRQRKRDMVSGEVAGFLE
                     GFKSSGAELIRGSGRFTGPKTLEVQLNDGGTRLLTADKVFLNIGTHSAMPNIPGLEAS
                     KPLTHIDILEIDYVPSHLVVLGGGYVGLEFAQAYRRFGSAVTVIDAGPQIISREDTDS
                     AEAIARILSDEGIRILAGAKIIKVEGSSGKAVTLSLQTSGSEERIEASDILVATGRVP
                     NTAGIGLEGAGVALDDRGYIRVNERLETSAPDVWAMGECAGSPQFTHASVDDFRIVKD
                     NLAGGARSTKNRLVPYCLFTDPPLAHVGLSERDAQRQGISVRVAKLPTQAVLRAHTTD
                     EKLGFLKILIDDSDHIVGFTMIGAEAGEVMMAAQTAMLAGLPYPKLRDAVIAHLTMAE
                     GLGPLLGNVPEKASGA"
     gene            complement(5834..6757)
                     /locus_tag="VGB93_13410"
     CDS             complement(5834..6757)
                     /locus_tag="VGB93_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0147114.1"
                     /translation="MMERQPLMLYDTVLLRTFAAVCETGSFTKAARVVNLTQSAVSLH
                     VKRLEEQVGAQLLRREPQGVSATEQGEVLLSYARRILALHKEAERRLGGDTGGLIRIG
                     APEYFDLQTLSSLLREFSAQYPALRLQVELGIGPDIAAMLEAGELDLAILSREIGEGD
                     GIALSREKRIWAASHAMQLDPDEPAPLALYPANCRWRQVVLDRLDRAGRPWTVAVQSS
                     GIAGILAALNAGLAITIFPESSLPDALKPIGNAAALPPLPDFEFVLRRSAVRSGAVDH
                     LAQMIASFFQLSAALRPGVAGDNDAHAHLAI"
     gene            6881..7321
                     /locus_tag="VGB93_13415"
     CDS             6881..7321
                     /locus_tag="VGB93_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147115.1"
                     /translation="MKLRRKRPIALILFAVLTTGPGLAQSVKPEATPPDPEAESRAAM
                     KDQVHDPRVEKSQKGGNDWGMWDIGRPHPMQGKGEFGGHDVIGFAAGKLIYTDCSLYW
                     NDPDKHKILCFSSQASLVYFLDAPRTNEMRAEKRWREMSKKPAS"
     gene            complement(7346..9364)
                     /gene="shc"
                     /locus_tag="VGB93_13420"
     CDS             complement(7346..9364)
                     /gene="shc"
                     /locus_tag="VGB93_13420"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY0147116.1"
                     /translation="MRREDNGPALQAAVRAAANWLVEHQKPDGHWVGRAESNDCMEAQ
                     WCLALWFLGLENHPLRPRLAQAILETQRPDGAWEIYLGAPNGDINTTVEAYAALRSMG
                     HRDDEPALERARTWIASKGGLRNVRVFTRYWLALIGEWPWEKAPNIPPEVIWFPKWFT
                     FSIYNFAQWARATLMPLSILSARRPSRPFPPENRLDALFPEGRDKFDYELPAKAGAGA
                     WDRFFRGAEHALHVLQNFGHKRGWNLQRNGAVRHVLEWVIRHQDSDGAWGGIQPPWIY
                     SVMALYTEGYAVDHPVIARSLAALDEPAWRVDRGEATWIQATNSPVWDTILTLMAFDD
                     AGMLEEHPEPVEKAVQWVLDRQVRVPGDWSIKLPDVRPGGWAFEYANNFYPDVDDTAV
                     ALIALAPFRNEPKWRERGIEDAIQRGIDWLIGMQSEGGGWGAFDKDNNKEILTKIPFC
                     DFGEALDPPSVDVTAHVIEAFGKLGLPRSHPTIVTALEYLKREQEPDGAWFGRWGVNY
                     IYGTGAVLPGLAAMGEDMSQPYIGRACDWIVSRQQENGGWGESCASYMDREAAGRGNP
                     TASQTAWALMALLAVDRPQDCAAIENGCLFLMRHQADGTWIEPEYTGTGFPGYGVGQS
                     IKLDSPLLRERLMQGPELSRAFMLRYDLYRQCFPLMALGRALRQRPQS"
BASE COUNT         1963 a         2874 c         2870 g         1774 t
ORIGIN      
        1 ctccttcgat gcgcgaactg tcttttcaac catctcgaac cttcatcgaa accgcccgcg
       61 actctgcacc ttcccgctgc gcggttcaac gcgattgcga cttgaagctg gaggtgtctt
      121 ctgttgtcgg ggctatgcgg cagtgcggga agacggtcca cgccactccg ccggacggat
      181 cagttcggcg aattatccgc gtggcagagc ttcggcattg tcggcgcgtt gaacttttcg
      241 aactggccgc acgacgtgca gccgctgaca aagagcgaca aagtaacaat tgcggcgagc
      301 gcgagaacgc gaattggcac cgttaacccc ctaaatcaca cgctaaacta gcataagccc
      361 gatttttttg cacctgcgcg agcattgacc gattttgtag agttaaattg catttccgag
      421 actgaattga acaagtatta acccagctcc gatagattaa attcaaattg gtctagagtt
      481 tttgaaatgc accggagtct gcaatgcagg agcatagaac taccgaacgc gtcaggagct
      541 ttctgcgagc ccaaatcgtt ttcaataatc gcatgacgac gatcgagtgc atcatcaaga
      601 attattcggc ggcgggcgca aaaatcgcgc tcaacgacac gctgaccgtg ccgaccgagt
      661 tcgatattta tatacctgcc aagcaacgca atcatcacgc gcgactcgta tggcgcgata
      721 aggatgcaat tggcgtgaac ttcatcgaag ccggggccgc agtcgcgaca aagacgccag
      781 cggccgccgc cgcggaccgc ctgcgcgagc ttgaattgca aaacgccgaa cttaagagtc
      841 gcgtgcgtga actgtccaag aggctggaag accttggcca ggacccgaat atcgcggcct
      901 gagctccgta cgtcattcca tttcccgcac gcgcccatcg tccagcgtaa ctctgcgcgt
      961 catctgcgcc gccaacgtct ggctgtgcgt tgcgaccagc gtcgccaggc ctgtcgcctg
     1021 gaccagcgtc tcgagcgacg cgaagacatg atccgccgta tgcggatcga ggttgcccgt
     1081 cggctcatcc gcgagaagga ggcccggcgc gttagcgact gcacgcgcaa tcgcgacccg
     1141 ctgctgttca ccacctgata attccgtcgg ccgatgctta gcgcgcgcca cgagaccaag
     1201 ataatcgaga agttcgagcg cgcgcacctc cgccttacgt cgcgagagcc cggcgatcat
     1261 ttgcggcagc atgatattct cgatggccga gaattccggc aggagatgat gaaactggta
     1321 gacgaagccg atggcgttgc ggcgcagcgc cgtgcgcgaa tcgtcggaaa ggcgggtggt
     1381 cgcgacgccg ttgatgaaaa cttcgccgct atccgggcgt tcgagcagac cggcgatatg
     1441 cagaagcgtc gatttgccgg tgccagacgg tgcgaccagc gccacggact gtccgggaaa
     1501 gagcgcaaaa tccgcatcgg ccagcaccga aagcgcctct tcgccttgct tgtagacgcg
     1561 ggtgatgcct ttgagctgaa gaacaggttc gctcatgtca ctcgtaacgt agggcttcga
     1621 cggggtcgag cttggccgcg cgccaggacg gataaagtgt cgccaaaatc gacagagcca
     1681 gggtcatgca gacgaccgtc gcgacatcgc gcggatcgac caccgagggc agccgggaga
     1741 ggaagtaaag ttccgccggg aagagattgg cgtgcagcac gtggttgagg aattgccgga
     1801 tcggttcgac gttttcggcg acgaggaggc cgagcagaaa tccggcaagc gtcccgacaa
     1861 tgccgatcga tgcgccggtg atcaggaaga tgcgcatgat cgcgccgcgc gtcgcgccca
     1921 tcgtgcgcag gatggcgaca tcatggcttt tgtccttcac cagcatgatg aggccggaga
     1981 tgatgttgag cgacgcaaca agcacgatca gggtgaggat gatgaacatc acgttgcgct
     2041 cgacattgag cgcatcgaaa aaggtcttat tgcgctcacg ccaatcggtc atgatgattg
     2101 gccgcgtcac cgtcgtttca atcttatcgc ggatcgcgtc gatgttctcg ggatcattca
     2161 ggaagagttc gacaacactc acctgtttgt cgagattgaa aaaagcctgc gcttccggca
     2221 gcggcatata gacgaaaatg ccgtcgaatt cggccatgcc gatctcgaaa atcgcgacga
     2281 tcttataggc tttgacgcgc ggcgcgacac caaatggcgt tcgcgcgcca ttggccgtca
     2341 tgatcttgat cttgtcgcca atcctcacat tgaggctttc ggcgaggcgc tgcccgatgg
     2401 caacaccatc gttcgcgtca aatccgttaa gcgtaccgga tcggatagtc cccgagacgc
     2461 cgggcagatg tttgatgtcg gcttcctcaa tgccgcggac caaaatgccg ctgccgtttt
     2521 gcgcgaagtc cgacgcaccg aaggcggcgc cctctaccat cggaatcgcg agatcgacgc
     2581 cgggcacctt ctcgacctgc ttggcgacct gattgtaatc ggtaagcggc gcatcagccg
     2641 cctgcaggaa gatatggccg ttgatgccga cgatcttgtt cagcaaatcg cgatgaaagc
     2701 cgttcatcac cgacatcacg acgatcaagg tcgcaacacc aagcgtgatg ccgagaaagg
     2761 aaaatccggc gatgacggac acgaagccac tggagcgccg cgccctcaaa tagcgcgtcg
     2821 caatcgtcca ttcaaacggc gcaaacatgg ccatcaggcg cttatccctc gcgcggttca
     2881 gccggcaaac cgggaaatca cagcttcgag ggagaggttt tcgcggatgc cggtgctgcg
     2941 ggttttcact tcgacctgtc cctcggcgag gcctttaggc ccgataatga tctgatacgg
     3001 caagccgatg agatcgaggc gggcaaactt cccccccggc cgctcgtccg tatcgtcata
     3061 aagcacgtca atcccggctt tttggagggc gtcgtagatt tttagcgagg ccgcatccgt
     3121 cgctgcgtcg ccgaccttga gattggcgat gccgatacgg aacggagcca ccgattcggg
     3181 ccagatgatg cccgcttcgt cgtgccccgc ctcgatcagc gccccgacaa gacgcgagac
     3241 gccgacgcca taggagccgc caagaaccgg ccgctcgacc ccgtccggcc cggtgacgac
     3301 ggccttcatc ggcttcgaat atttatcgcc gaaatagaaa acctggccga cttcgatgcc
     3361 gcgtttttgc aagcgcttgt ccgcgggcgt ctcgcgctcg aaccgcgccg cgtcatgcac
     3421 gtcgtcggtc gccgcataaa gcgacgtcca ttggtcgatg atcggcgtga ggtccgaatc
     3481 ataatcgacg tcggccgtcg ggatgggcag gtcgagcaca tcggaattga taaagacgcc
     3541 cgactcgccg gtgtcggcga ggatgatgaa ttcgtggctc tgatcgccgc cgatggggcc
     3601 cgtctcggcc cgcatcggga tggctttgag ccccatgcgg gcgaagatgc gcagataggt
     3661 gacgaacatg cgctgatagg agcgccgtgc cgccgcctcg tcgatatcga aggaataggc
     3721 atccttcatc aggaattcgc gcccgcgcat gacgccgaaa cgcggccgct gctcatcgcg
     3781 aaacttccac tgtatgtgat agaggttttt cggcaagtcg cgataggagc ggacggaggc
     3841 gcggaagatt tccgtgatca tgtcctcgtt ggtcggcccg tagagcaatt cgcgctcgtg
     3901 ccggtctttt atgcgcaaca tctcggggcc gtaggcatcg taacggcccg tctcgcgcca
     3961 gaggtcggcg agttgcaacg tcggcatcag caattcgatg gccccggcgc gatccatctc
     4021 ttcgcggacg atctgcgtca cgttctcaag aacgcggaag ccgagcggca gccaggcgta
     4081 gattccggcg gcctcctgcc ggaccatgcc ggcgcgcagc atcagccggt gcgagacgat
     4141 ctcggcctct ttgggatttt cacggagaat tggcaggaaa tagcgggaaa gacgcattca
     4201 gattgcactg caaggctgga ggaccggatg ggccgcaata gcttgcggcg gcggccgaaa
     4261 caatccgaaa ggggcggaaa aggcaatata gccgcgatca agcccgcctc ggctgaagtc
     4321 gtgtaacttc atccaacatc ggcacgtatc aagacaatca gccacgatag tcggcgtcgg
     4381 caagcttcgc acaggagtgc acgtcgtcgc gcaaacggag ggtattatgt cggcagcgga
     4441 acatctcgac gtcctggtcc ttggcagcgg acaggcaggc aatttgatgg cctggcacat
     4501 ggcgcgatcg ggataccgaa ccgctttgat tgaacgccgg tggatcggcg gctcctgtcc
     4561 caacttcgcc tgcatgccga gcaagaatga ggtttggagc gctcgggtcg ctcatctcgt
     4621 ccgtaacgcc gggcatttcg gaacgatcac gacaccgggc aagatcgaca tgggccaagt
     4681 ccgccagcgg aaacgcgaca tggtgagcgg cgaggtcgcc ggattcctgg agggtttcaa
     4741 gtcgagcggc gccgaactca tccgcggaag cggccgtttt actggcccta aaacgctcga
     4801 agtgcaactc aacgatggcg gcacgcgcct tctgacggcc gacaaagtgt ttctcaacat
     4861 tggcacgcac agtgcaatgc cgaatattcc tggccttgag gcctcgaagc ccctcactca
     4921 catcgacatt ctcgaaatcg attatgtgcc ttcgcatttg gttgttctgg gcggcggcta
     4981 tgtcggcctt gaattcgcgc aggcttaccg gcgttttggc agcgcggtga cggtcatcga
     5041 cgccgggcct cagatcatca gccgcgagga taccgacagc gccgaagcca ttgctcgaat
     5101 tctcagcgac gaaggcatcc ggattttagc tggagcaaaa atcatcaagg tcgagggatc
     5161 gtccggcaaa gctgtcaccc tctcactaca aactagcggc agcgaagagc ggatcgaagc
     5221 gagcgatatc ctcgtcgcca ctggccgcgt ccccaacaca gccggcatcg gtcttgaggg
     5281 ggccggcgtt gcactggacg atcgcggcta tatccgcgtc aatgaacgat tggagacgag
     5341 cgcgcctgac gtgtgggcaa tgggcgaatg cgccggcagt ccgcaattta cgcatgcgtc
     5401 ggtcgatgat tttcgcatcg tcaaagacaa tctggccggg ggagcgcgca gcacaaaaaa
     5461 tcgcctcgtg ccttattgcc ttttcaccga tccgccattg gcgcatgtcg gcttgagcga
     5521 acgcgacgcg cagcgtcagg gtatttccgt ccgcgtcgcc aaactgccga cgcaggccgt
     5581 gctgcgcgcc catacgacgg acgagaagct tggatttctg aagatcctga tcgacgacag
     5641 cgatcacatc gtcggcttca caatgattgg cgcggaagct ggtgaggtca tgatggccgc
     5701 gcagacggcg atgttggccg gcctgcccta tcccaagctt cgcgacgccg tgatcgcgca
     5761 tctgacgatg gcggaaggac tcggcccgct gctcggcaac gtgccagaaa aagcgtcggg
     5821 cgcctgagtt cgcctaaatt gcgagatgcg cgtgcgcgtc gttgtcgccc gcaacaccag
     5881 gtcgcagcgc cgctgaaagc tggaaaaaac tggcgatcat ctgcgcgaga tgatcgaccg
     5941 ccccgctgcg caccgcgctt cgccgcagga cgaattcgaa atccggcagc ggcggcagcg
     6001 ccgcggcatt gccaatgggt ttgagcgcgt ccggcaggct gctttccggg aagatcgtga
     6061 tcgcgaggcc cgcgttgagc gccgcgagaa taccggcgat cccggagctt tgaaccgcga
     6121 ctgtccaggg gcgccctgcc cggtcgagtc ggtccaggac cacctgccgc caccggcaat
     6181 ttgccgggta aagcgcgagc ggtgccggct cgtccgggtc gagctgcatg gcgtgactgg
     6241 cggcccagat acgcttctcg cgcgacaacg cgataccgtc gccctcgcca atctcccggc
     6301 tcagaattgc gagatcgagt tcgcctgcct caagcatggc ggcgatatcg gggccgatac
     6361 ccagttcgac ttgcagtcgc agtgccggat attgcgcgga aaactcccgg agcaatgacg
     6421 acaacgtttg cagatcgaaa tattccggcg ccccgatccg gatgaggccg ccagtgtccc
     6481 cgcccaggcg acgctcggcc tctttgtgca aggcgagaat gcgccgggca taggagagca
     6541 gaacttcgcc ctgctcggtc gcactgacgc cctgcggctc acgtcggagc aactgcgcgc
     6601 cgacctgttc ttcgaggcgc ttaacgtgca ggctgaccgc agattgggtg aggttcacca
     6661 cgcgcgccgc cttggtgaag ctgcccgttt cgcaaacagc tgcgaacgtc cgtaagagca
     6721 ccgtgtcgta aagcatcagc ggttgtcgct ccatcatgcc cataagtcgg gctcatcgcg
     6781 cgcatgacgc atcttcgata tccgcgcgga gaagcaaatg tatagttccc cgccttgaga
     6841 cgccattgac attgccggcg ctgatcagga gacgacgagc atgaaattgc gccgcaaaag
     6901 gccgatagcg ctgattttat tcgcagtcct gaccacaggg cctgggctcg cgcagagcgt
     6961 gaagcccgaa gcgacaccgc cggatccgga agccgaatcc cgggccgcga tgaaagatca
     7021 agtgcacgac ccgcgcgttg aaaaatcgca aaaggggggc aacgattggg ggatgtggga
     7081 tattgggcgc ccgcacccca tgcaagggaa aggcgagttt ggcggccatg acgtgatcgg
     7141 cttcgccgcg ggcaagctta tctacaccga ctgctcgctc tattggaacg atcccgacaa
     7201 gcacaaaatc ctttgcttct cgtcgcaggc ctcgctcgtc tatttcctcg atgcaccgag
     7261 gaccaacgag atgcgcgccg aaaagcgctg gcgcgagatg agcaagaagc cggcgagttg
     7321 agtttcggac tgcttgcgcg agagcctagc tctgcggacg ttggcgcaaa gcgcgtccca
     7381 gcgccatgag cggaaaacac tgccggtata aatcgtagcg cagcatgaac gcgcgggaca
     7441 gttccggacc ctgcatcagc cgttcgcgca gcaaaggcga gtcgagtttg atcgactggc
     7501 caacgccata gccggggaat cctgtgccgg tatattccgg ctcgatccag gtcccatccg
     7561 cttgatggcg catgaggaag aggcagccgt tttcgatggc tgcgcaatct tgcgggcgat
     7621 ccaccgccag gagcgccatg agcgcccagg ccgtttgcga cgccgttgga ttgcctctgc
     7681 ccgccgcttc acgatccatg taggacgcgc agctttcgcc ccagccgccg ttttcctgct
     7741 gacggctgac gatccagtcg caggcgcgcc cgatataagg ctggctcata tcctcgccca
     7801 tggcggccaa gcccggaagg acagcgcccg tcccgtaaat ataattgacg ccccaacgcc
     7861 cgaaccacgc gccgtccggc tcctgctcgc gcttcagata ttcaagcgcg gtgacgatcg
     7921 tcgggtgaga gcgcgggagg ccgagtttgc cgaaggcttc gatgacatgc gccgtgacat
     7981 cgacagacgg cggatcgagc gcctcgccga aatcgcagaa cggaattttc gtcagaatct
     8041 ctttgttgtt gtccttgtcg aaggcccccc agccgccgcc ctcgctctgc atgccaatca
     8101 gccagtctat gccgcgctgg atcgcatcct caatgccgcg ttcgcgccat ttcggctcgt
     8161 tgcggaatgg cgccagcgcg atgagcgcca cggctgtatc atcgacgtcc ggataaaaat
     8221 tattggcgta ttcgaaggcc cagccgccgg ggcgcacatc tgggagctta atcgaccagt
     8281 cgccaggaac acgtacctgc cggtcgagca cccattgcac ggctttctcg accggttccg
     8341 gatgctcttc cagcatcccg gcatcgtcga aggccatgag ggtcaaaatc gtatcccaga
     8401 cggggctgtt ggtcgcttga atccaggtcg cctcgccgcg atccacgcgc cacgcgggtt
     8461 cgtcgagcgc cgcaaggctc cgggcaatca ccggatgatc gacggcatag ccttccgtat
     8521 agagcgccat aacgctgtag atccacggcg gctggatgcc gccccaggcc ccgtcggaat
     8581 cctgatgacg gatgacccat tctaggacat ggcggacggc gccgttacgc tgcagattcc
     8641 agccccgctt gtgtccgaaa ttctgcaaga catgcaacgc gtgctcggcg ccgcggaaga
     8701 atctgtccca cgctccagcg ccggccttgg cgggcaattc gtaatcgaac ttgtcgcggc
     8761 cttcggggaa cagcgcatca agccggtttt caggtgggaa cggacggctt ggccggcgcg
     8821 ccgacaggat cgagagcggc atcaaggtcg cccgcgccca ttgcgcgaaa ttatagatcg
     8881 agaacgtgaa ccacttagga aaccagatga cttcaggcgg aatgttcgga gccttttccc
     8941 acggccattc gccgatcagc gcgagccaat agcgggtaaa cacccgcacg ttgcgcaggc
     9001 cgcctttgct ggcaatccac gtccgcgctc gctcaagcgc cggttcgtcg tcacgatggc
     9061 ccatcgagcg caatgcggca taggcctcga cggtcgtatt gatgtcgccg ttcggggcgc
     9121 cgagatagat ctcccaggcg ccgtcggggc gctgcgtttc aagaattgcc tgcgccagac
     9181 gcggccgcaa cgggtgattt tcgagaccaa ggaaccacag cgccaggcac cattgcgcct
     9241 ccatacagtc gttcgattcg gcgcggccga cccaatgccc gtccggcttc tggtgctcga
     9301 cgagccaatt ggcggcggcc cggacagcgg cctgcaaggc cggcccgtta tcctcgcgcc
     9361 tcatatcgct gaggccgtcg accgcaaaac ttttggcttg ctgcacgttc atttctgaaa
     9421 aggatactcc cgcgaaggcg aagaccgact gcaatatgtc attttgctct gcggtgcaaa
     9481 a
//
LOCUS       DASWOG010000655         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_200563,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000655 DASWOG010000000
VERSION     DASWOG010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200563"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VGB93_13425"
     CDS             <1..1152
                     /locus_tag="VGB93_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0147117.1"
                     /translation="IGTWLTHLIGHPLVPLSFRRQRYEADFRFSLARLREYTEQIALL
                     SGEDAEKRSLKTRFAAIMSNYFEIIACRKRLTAFTASYSQISPFIPYIVSAPFYFAGK
                     ITLGIMTQTANAFSNVNSALTFVVSYYVNLADYKSVLDRLTSFDASIEKAANYQFRAQ
                     PTPGSATPGNVSLAEVTLRLPDGRTIVEDANLDLVSEIPALFMGPSGSGKSTLFRAIA
                     GIWPYGEGKIDLPPSAKVMLLPQRPYLPLGTLREALAYPATAERYDDATIRAAVEAAQ
                     LGHLMSKLDVEGMWQQQLSGGEQQRLSIVRALLEKPDWLFLDEATTAMDEPMEAAIYQ
                     TLREKLPKTTIVSIGHRATIEKYHTRRLEMQPAGAGMFTPKDGVKTAAE"
     gene            complement(1212..>1972)
                     /gene="hisG"
                     /locus_tag="VGB93_13430"
     CDS             complement(1212..>1972)
                     /gene="hisG"
                     /locus_tag="VGB93_13430"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0147118.1"
                     /translation="GVTGEDLVRESIPDADSKCELLAPLGFGHANVIVAVPQAWIDVR
                     TMADLEDVAVAFRARRGERMRVATKYVNLTRHFFAERHVTDYRIVESLGATEGAPAAG
                     TAELIVDITTTGGTLAANALKILDDGVILRSEANFVASLTAAWTDETREAARIILSRI
                     AAEEVARTTREVSARVAQADVELFAHAEVMFAARARRHLSDGWLTLTCPKDKVSALAD
                     WLLMQGAAEVTVAQLDYVFAEKNKLYEKLAARLP"
BASE COUNT          380 a          594 c          598 g          400 t
ORIGIN      
        1 attggcacgt ggctgacgca tttgatcggc catccgctcg tgcctttgtc gttccggcgc
       61 cagcgttacg aagccgattt ccgtttttca cttgcgcgtt tgcgtgaata tacggagcag
      121 atcgcgttgc tttcaggcga agacgcggag aagcgttctc ttaagacgcg ttttgcggcg
      181 atcatgtcga actatttcga gatcatcgcc tgccgcaagc ggcttaccgc gttcacggcg
      241 tcctattcgc agatcagtcc attcattcct tacatcgtct cggcgccgtt ctatttcgcc
      301 gggaaaatta cgctgggtat catgacccag accgcgaatg ccttcagcaa cgtcaatagc
      361 gcgctgacct tcgtcgttag ctattatgtg aatctcgcgg attacaaatc cgttctcgat
      421 cgtctgacgt cgttcgacgc ttcaattgaa aaggccgcga actatcaatt tcgcgcgcaa
      481 ccgacgccgg ggagcgctac gccgggcaat gtcagcctcg ctgaagtgac attgcgcttg
      541 cccgacggcc gcacgatcgt cgaagacgca aatctcgatc tcgtgtcgga aattcccgcc
      601 ctattcatgg ggccgtccgg atccggcaag tcgactttgt tccgcgccat cgccggcatc
      661 tggccttacg gcgaaggcaa gatcgatctg ccaccgtctg cgaaggtgat gctcttacct
      721 cagcggccat atctgccgct tggcacattg cgcgaggcgc tggcctatcc ggcaacggcc
      781 gagcgatatg acgatgcgac gatacgtgcg gccgtcgagg cggcgcagct cggccatctg
      841 atgagcaagc tcgacgtcga gggtatgtgg cagcagcagc tttcgggcgg cgagcagcag
      901 agattgtcga tcgtgcgcgc attgctcgaa aagcctgatt ggctcttcct tgacgaggcg
      961 acgacggcga tggacgagcc gatggaagcg gcgatctatc agacgctgcg cgaaaaattg
     1021 ccaaagacga cgatcgtctc cattggccat cgcgccacga tagagaaata ccatacgcgc
     1081 cggctcgaaa tgcaaccggc gggcgcgggc atgtttacgc cgaaggacgg tgtgaagacc
     1141 gcggcggaat aaaaggtatg cggccgcatc ggaccgtccg aaaaccgctt cgcacttttc
     1201 ggtccgatgc tctaaggcag ccgcgcggcg agcttttcgt agagcttgtt cttctcggcg
     1261 aagacataat cgagctgcgc gaccgtgact tcggctgcgc cctgcatcaa tagccagtcg
     1321 gcgagcgccg acactttgtc tttcgggcag gtgagcgtca gccagccatc ggagagatgc
     1381 cggcgcgccc gcgctgcgaa catcacctcg gcatgggcga agagttcgac atcggcctgc
     1441 gcaacccgcg ctgagacttc gcgtgtcgtg cgcgcgactt cttcggccgc gatgcgtgag
     1501 agaatgatcc gcgcagcctc gcgtgtctcg tctgtccacg cggccgtcag cgacgcgacg
     1561 aaattcgcct cggaccgcag gatcacgcca tcgtcgagga ttttgagcgc attcgctgcg
     1621 agcgtgccgc cggtcgtggt gatatcgacg atcagctccg ccgtgccggc cgcgggggcg
     1681 ccttcggtcg cgccgaggct ttcgacaata cgatagtcgg tgacgtggcg ctcggcgaaa
     1741 aaatgccgcg tgagattgac gtatttggtc gcgacacgca tccgctcgcc gcgtcgggcg
     1801 cgaaaagcca ccgcaacgtc ctcgagatcg gccatggttc gtacatcgat ccaagcctgc
     1861 ggcacggcaa cgatgacatt ggcatggccg aagccgagcg gcgcgagaag ttcgcatttc
     1921 gaatcggcgt cgggaatgct ttcacgcacc agatcctcgc cggtgacgcc ga
//
LOCUS       DASWOG010000656         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_363897,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000656 DASWOG010000000
VERSION     DASWOG010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363897"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGB93_13435"
     CDS             complement(<1..251)
                     /locus_tag="VGB93_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0147119.1"
                     /translation="MSQSEYEDLPFEPGTVWLVGAGPGDPGLLTLHALSGLRRADVIV
                     HDALVSDHVLTLASPNAKREFAGKRGGRPSHSQSDISLRL"
     gene            complement(280..1038)
                     /gene="cobM"
                     /locus_tag="VGB93_13440"
     CDS             complement(280..1038)
                     /gene="cobM"
                     /locus_tag="VGB93_13440"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEY0147120.1"
                     /translation="MTIHFIGAGPGAPDLITLRGRDLIARCNVCLYAGSLVPRELLAY
                     CPAGARIVDTAPLALEAIVAEFKRATDEGEDVARLHSGDLSIWSAMGEQLRELDALGI
                     PYSVTPGVPSFAAAAALLQRELTLPEVAQSLVLTRTGGRASSMPSKETLANFAATGTT
                     LAIHLSIHALDRVVEELSPYYGEACPVAIVYRASWPDERVIEAQLGTIGAAVIEDKPE
                     RTALILVGPALAARDFRKSALYDIDYQRRFRGAQ"
     gene            complement(1035..1415)
                     /locus_tag="VGB93_13445"
     CDS             complement(1035..1415)
                     /locus_tag="VGB93_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEY0147121.1"
                     /translation="MIAIGIGCRRGIAKEAFVEMIRQALSLIGDAAGVVELFTIEDKH
                     DEAGLLAAAADLRLPIRFLSRAALQSVEASVVTPSPQAETAFGVASVSEAAALAGAGC
                     GAVLIVPRIVGQGVTCAVARGGGR"
     gene            complement(1412..2626)
                     /gene="cbiE"
                     /locus_tag="VGB93_13450"
     CDS             complement(1412..2626)
                     /gene="cbiE"
                     /locus_tag="VGB93_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEY0147122.1"
                     /translation="MGSPSPTRWLTIIGIGEDGLDGLSPAARRLLSQADFIVGGARHL
                     AFLGETKAKTLPWPTPFERGIDEIKARRGTPVCVLASGEPFYYGVGATLATHVCADEM
                     ICLPAPSSLSLAAARLGWPLQDCDVVSLHGRAFERIIPHLRPNARLLILSWAASTPEK
                     LAYLLVARGLGGSRFIVLEALGGPNEHIQESTAGAFKPREIATLNLVAIEIVAAEGSA
                     FIPLTPGLPDAWFESDGQLTKREIRAVTLSALAPWPGALLWDIGAGSGSIGIEWMLAH
                     PRNRAVAIEVREDRAARITRNAAALGVPDLEILTGNALALMQNLPPPDAIFVGGGGGA
                     MIDAAWTYLVTRRRFVANAVTIETQSHLAACHAQYGGELVSLSVAHPQIVGGYRSWRP
                     TLPVVQWAVTKS"
     gene            2646..3401
                     /locus_tag="VGB93_13455"
     CDS             2646..3401
                     /locus_tag="VGB93_13455"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HEY0147123.1"
                     /translation="MLRVLILGGTTQASALAAKLAGGARYDAILSLAGRTKNPLLADI
                     RHRIGGFGGSAGLANYLLTEAIDVLIDATHPFAEQISTNAVIAARATNTPLAIFTRPA
                     WIPSAGDRWREFPDAGSAARALGEKPRRVFLTIGRQQLAAFEIAPQHDYLIRSIDAPK
                     SLPALPHHKLLLARGPFTFEDEQALMRDAGIEILVTKNSGGTATRAKLDAARALGIDV
                     HMIARPQSPDVPTFETLDAILQYLEAAIHRTTP"
     gene            complement(3383..4147)
                     /gene="cobJ"
                     /locus_tag="VGB93_13460"
     CDS             complement(3383..4147)
                     /gene="cobJ"
                     /locus_tag="VGB93_13460"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEY0147124.1"
                     /translation="MKAGHLAIVGLGPGPRGLMTFEAEAALRRATDLVGYGPYVDRLT
                     LSPGQTRHASDNRVEIDRARHALDLALAGARVAVVSGGDPGVFAMAAAVFEAIEQGDS
                     RWRNLDIEVHPGISAMLAVAARLGAPLGHDFAAISLSDNLKPWAAIAKRLTAAAEADF
                     VMAFYNPASKARPHLIHDVFKLLRGLKAPETIVVFARAVSRPDEKIELTTLAEADPSI
                     ADMSTLILVGSSETRRITRGDGGSWVYSPRSYGVVR"
     gene            complement(4144..4872)
                     /locus_tag="VGB93_13465"
     CDS             complement(4144..4872)
                     /locus_tag="VGB93_13465"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEY0147125.1"
                     /translation="MSAGKLIGVGLGPGDPELLTVKAVKAIKSALVVAYFAKAGRRGN
                     AREIADCWIAHEQTEVPLYYPMTTETRFDHPVYIETLRAFYEASCATLATHLEAGRDV
                     ALLAEGDPFFYGSFMHIYVRLRERFETVIVPGVPGMAGCWSVAETPMTWGDDVLIVLP
                     ATLSEDEIVERLSHCDAAVIMKLGANLPKARNAVLRAGRVASALYVERGTMPGEKVMK
                     LAEKTDESAPYFSLILIPGEGRRP"
     gene            complement(4869..5495)
                     /locus_tag="VGB93_13470"
     CDS             complement(4869..5495)
                     /locus_tag="VGB93_13470"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEY0147126.1"
                     /translation="MNYSYIRDGAEIYRRSFAIIRAESDFARFTAEQEPVAVRVIHAS
                     GQVEAARDLVFSPDFVGAARTALQNGAPILCDAKMVAGGITRARLPADNQIICTLADP
                     AVPTLAIEIGNTRSAAALHLWKKHIADAVVAIGNAPTALFHLLELFEAGWPRPAAIIG
                     MPVGFVGAAESKEALINFADVPFLVARGRRGGSAMTVAAINALASTKE"
     gene            complement(5492..5995)
                     /locus_tag="VGB93_13475"
     CDS             complement(5492..5995)
                     /locus_tag="VGB93_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147127.1"
                     /translation="MHYVIDLGVPFGRLDASTLKRLADVAEASCGELRLTPWRAVLVV
                     GAEQPDAARLADTGLIFDESDPLRSVAACPGAPGCTSGTTATHADARRLAPFARQLGA
                     RGISLHVSGCAKGCAHPRSAPVTLVGREGRYDLVCDGTAADKPIAIGLEAHQLDLALQ
                     QFTKIGA"
BASE COUNT         1212 a         1961 c         1836 g         1028 t
ORIGIN      
        1 agccgcagcg aaatatcgct ctgactgtgc gagggccggc cgccgcgctt gccggcgaat
       61 tcgcgctttg cgttcggcga cgcgagcgtc aatacatgat ctgacaccag cgcgtcatgc
      121 acaatcacat cggcccgccg caaaccgctc aacgcgtgta gcgtcaacag ccccggatca
      181 cccgggccag caccaacgag ccagaccgtc ccgggctcga acggcagatc ttcgtattcg
      241 ctctggctca ttttggctgc gtacccgcca tctgctttct cattgcgcgc cacggaatcg
      301 gcgctgatag tcgatgtcat acagcgcgct tttgcggaaa tcccgcgcgg cgagcgccgg
      361 gcccacaagg atcagcgcgg tccgttctgg cttatcttcg atgaccgcag cgccgatcgt
      421 gccgagctga gcttcgatca ctctctcgtc cggccagctc gcgcgataga cgatcgcgac
      481 ggggcaagcc tcgccataat agggcgagag ctcttcgaca acccggtcaa gcgcgtggat
      541 ggacagatga atggcgagtg tggtgccggt cgcggcgaaa ttggcgagcg tctccttgga
      601 cggcatcgac gaggcacgac cgcctgtccg ggtgagcacg agagattgcg cgacttccgg
      661 caacgtcaat tcgcgttgaa gaagcgcggc ggcggcggcg aagctcggca cgcccggcgt
      721 cacactatac gggatgccga gcgcatccaa ttcgcgcaat tgctcgccca tcgcgctcca
      781 gatgctgaga tcgccggaat gaagccgcgc gacatcctcg ccttcgtccg tcgcgcgttt
      841 gaattctgcg acaatcgctt cgagcgccag cggcgcggtg tcaactatgc gcgcacccgc
      901 cgggcaatag gcgagaagtt cgcgtggcac cagcgagccg gcataaaggc agacgttgca
      961 acgcgcgatg agatcgcgac cgcgcaatgt gatgagatcc ggtgcgcccg gccccgcgcc
     1021 gatgaaatga atcgtcatct acctcctcca cgcgcgaccg cgcatgtgac gccctgcccg
     1081 acgatgcgcg gcacgataag cacggcacca cacccggcgc cggccagcgc agccgcctcc
     1141 gaaacggagg cgaccccaaa cgcggtttcg gcttgtggcg acggggtgac aacgctcgcc
     1201 tcgacgcttt gcaaggccgc gcgagacaag aagcggatcg gcaggcgcaa atccgccgcc
     1261 gcggcgagca gccctgcctc gtcatgtttg tcttcgatag tgaacaattc taccaccccg
     1321 gcagcgtctc cgatcagcga tagagcctgt cgtatcatct caacgaatgc ctccttggcg
     1381 atcccacggc ggcagccgat gccgatcgca atcatgattt cgtcaccgcc cattgtacga
     1441 ccggcagcgt cgggcgccag cttcgatagc cgccgacgat ctgcggatgc gcgacgctga
     1501 gcgacacaag ttcgccgcca tattgcgcgt ggcacgcggc gaggtgcgat tgcgtttcga
     1561 tcgtcacggc gttggcaacg aaacggcgcc tagtcacgag atacgtccac gccgcgtcga
     1621 tcattgcgcc accgcctccg ccaacgaaga tcgcatcggg cggtggcaga ttttgcatca
     1681 aagcaagagc attaccggtc agaatttcca gatccggaac gccgagcgcc gcggcattgc
     1741 gggtgatacg cgcggcacga tcttcccgca cttcgatagc gacggcgcga ttgcgcggat
     1801 gcgccagcat ccactcgatc ccgatcgagc cagacccggc gccgatatcc caaagaagcg
     1861 cacccggcca cggcgccagt gctgaaagcg tgacagcgcg gatttcgcgc ttggtcaact
     1921 gcccgtctga ctcgaaccat gcgtccggca ggcctggcgt caaaggaatg aaggccgagc
     1981 cctccgcggc gacgatttca attgcaacga gattgagcgt cgcgatctcg cgcggcttga
     2041 aagctcctgc ggtgctttct tgaatgtgct cgtttgggcc acccaaagcc tccagcacga
     2101 taaaccgcga cccgcccagc ccgcgcgcga caaggagata cgcgagcttc tccggcgtgg
     2161 aggcggccca agaaagaatc aacagacgcg cgttcggacg cagatgcggg atgatccgct
     2221 caaacgcgcg cccgtgcaac gaaacgacat cgcaatcctg caacggccag cccaatcgcg
     2281 ccgccgccag actgagcgac gacggcgccg gcaagcagat catctcgtcc gcgcagacat
     2341 gcgtggccaa tgtcgcgccc acgccgtaat aaaacggctc gccggaggca agcacgcaga
     2401 ctggcgtgcc gcgccgcgcc ttgatttcgt caatgccgcg ctcgaatggc gtcggccacg
     2461 gcagcgtctt ggcctttgtt tcgccgagaa aggcaagatg gcgcgcgccg ccgacgatga
     2521 aatccgcttg cgacaaaagc cgtctcgcgg caggagaaag gccatcgagc ccatcttcac
     2581 cgatgccgat gatcgtgagc cagcgtgtcg gtgatgggct acccatggag ccaccccgca
     2641 aaatcatgct gcgtgttttg attcttggcg gcacgacgca ggcaagcgcc ttggcggcaa
     2701 agcttgccgg cggtgcgcgt tacgacgcga tcttgtcgct ggccggccgc accaaaaatc
     2761 cgttgctcgc ggatattcgc catcgcatcg gcggctttgg gggcagcgcg ggattggcaa
     2821 actatctgct tacggaagcg atcgacgttt tgatcgacgc cacgcatccg ttcgccgagc
     2881 agatttctac gaacgctgtc attgcggcgc gagcgaccaa caccccgctg gctatattca
     2941 cgcgcccggc ctggatcccc agcgcgggcg accgatggcg ggaatttccg gatgcgggga
     3001 gcgcagcacg cgccctcggt gaaaagccgc gtcgcgtctt tctcacgatc ggacgacaac
     3061 aactcgcggc gttcgaaatc gcgccacagc acgattatct cattcgctcc attgacgcgc
     3121 ccaagtcctt gcccgcattg ccgcaccaca aacttctgtt ggcgcgggga ccattcactt
     3181 ttgaagacga gcaggcgttg atgcgcgacg cgggcatcga aattcttgtc accaagaaca
     3241 gcggtggcac ggcgacgcgc gccaagctcg acgccgcccg cgcccttggt atcgatgtcc
     3301 acatgatcgc gcggccgcaa agcccggatg tcccgacatt cgagacgctc gacgccattc
     3361 tgcagtatct tgaagccgcg attcatcgca cgacgccgta gctgcgcggt gaatagaccc
     3421 aggatccgcc gtcgccgcgc gtgatgcgcc gcgtctcgct cgagccgacg aggatcaacg
     3481 tactcatatc cgcgatcgac gggtcggcct ccgcgagcgt agtcaattca atcttctcat
     3541 ccggccgact gacggcgcgc gcgaaaacga cgatggtttc gggagctttc agcccccgca
     3601 gcaatttgaa gacatcgtgg atcagatgcg gccgcgcctt cgaggcggga ttgtagaagg
     3661 ccatgacgaa atcggcttct gccgccgccg tcagccgttt cgcgatcgcc gcccaaggtt
     3721 tcagattgtc cgagagcgaa atcgccgcga aatcatgccc cagcggggcg ccgagacgtg
     3781 ccgcgacagc gagcatggcc gagatgccag gatggacttc gatgtcgaga ttacgccaac
     3841 gcgaatcgcc ctgctcaatc gcttcaaaga ccgctgcagc catggcaaag acgccgggat
     3901 caccgccaga cacaacggca acacgcgcgc cggcgagcgc cagatcgagc gcgtggcgtg
     3961 cccggtcaat ttcgacgcga ttgtcgctcg cgtggcgcgt ttggcccggc gaaagtgtca
     4021 gccggtcgac ataaggccca tagcccacaa gatcggtcgc acgacgcaaa gccgcctcgg
     4081 cttcgaacgt catcagcccg cgcggaccag ggccaagacc gacgatggcg agatggcctg
     4141 ccttcatggc cgcctgccct cgccgggaat gaggatcagc gaaaaatacg gcgcggactc
     4201 gtcggtcttc tcagcaagct tcatcacttt ctcgcccggc attgtgccgc gttcgacata
     4261 gagcgcactt gccacgcgcc cggcgcgaag aacggcgttg cgtgccttcg gcaggttagc
     4321 gccgagtttc atgatgacgg cggcatcgca atggctcaga cgttcgacga tctcgtcttc
     4381 cgaaagcgtc gccggcaaaa cgatgagaac gtcgtcgccc catgtcatcg gcgtttccgc
     4441 cacgctccag catcccgcca tgcctggcac gccgggcaca atcacggtct cgaaacgttc
     4501 gcgcagacgc acatagatgt gcatgaagga accgtagaaa aacggatcgc cttctgccag
     4561 caaggcgaca tcccggccag cttcgagatg cgtggcaagc gttgcgcaag atgcttcgta
     4621 gaaggcgcgc aatgtctcaa tgtaaacggg atgatcgaac cgcgtttccg tcgtcatcgg
     4681 ataatacagc ggcacttccg tctgctcgtg cgcaatccag caatcggcga tttcgcgcgc
     4741 attgccgcgg cgcccggcct tcgcgaaata ggcgacgacg agcgctgatt tgatcgcctt
     4801 caccgctttg acggttaaaa gctcgggatc acccggcccg agccctacgc cgatcaattt
     4861 tccggcgctc attctttggt gctcgcgagc gcgttgatcg cggccaccgt catggcgctc
     4921 ccgccgcgcc gtccgcgcgc gaccagaaat ggcacgtcag cgaaattgat cagcgcttct
     4981 ttcgattccg ctgcgccgac aaagccgacc ggcatgccga tgatggccgc gggccgtggc
     5041 caaccggctt caaaaagttc aagcagatga aataatgccg tcggcgcatt gccgatggcg
     5101 acgacagcat ccgcgatatg tttcttccaa agatgaagtg ccgcggcgga gcgcgtattg
     5161 ccaatctcga tcgcgagcgt aggaaccgcc gggtcagcca gtgtgcagat gatctgatta
     5221 tcggccggca accgcgcgcg cgtgataccg cccgcgacca ttttcgcgtc gcagagaatt
     5281 ggggcgccat tttgcagggc tgtgcgcgct gcaccgacaa agtcaggcga aaagacgaga
     5341 tcgcgcgcgg cctcgacctg cccgctcgcg tgaatgacgc ggaccgcgac cggctcctgc
     5401 tcggcggtaa agcgggcaaa atctgattcg gcgcggatga tggcaaagga gcggcggtaa
     5461 atctccgcgc cgtcgcggat ataggaatag ttcatgcgcc gattttcgtg aattgctgca
     5521 aagcaaggtc caattgatgg gcttcgaggc caatggcaat tggcttatcc gccgcggttc
     5581 catcacacac aagatcatag cggccctcac ggccgaccag cgtaaccggc gccgacctcg
     5641 gatgcgcgca gcccttggcg cagcccgaaa catgcaagga aatgccacgg gcacccaatt
     5701 gccgggcgaa gggcgcaagc cggcgcgcat cggcgtgcgt cgcggtcgtc ccgctggtgc
     5761 aaccgggtgc gcccggacac gcggcgacag agcgcagagg atcgctctcg tcgaagatca
     5821 atccagtgtc ggccagacgt gccgcatcgg gttgctcggc accgacaacg aggacggcgc
     5881 gccaaggtgt gagacgcaat tcgccacagc tcgcctcggc aacgtccgcc aatcgtttta
     5941 gcgtcgaggc atcgagccgt ccgaaaggga ctccgagatc aataacataa tgcacgccgt
     6001 taggctaagc gccgatcggg tgcggggcgt gcttttt
//
LOCUS       DASWOG010000657         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_363915,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000657 DASWOG010000000
VERSION     DASWOG010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363915"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1324)
                     /locus_tag="VGB93_13480"
     CDS             complement(347..1324)
                     /locus_tag="VGB93_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147128.1"
                     /translation="MNERKVAVYYAWSRPGEIEAPLAVIEDRFPSLFESRRLVYPRFH
                     ELSDPVRFDQSIAGFLDHIMKANFIDFAEQASTQTGHPVLEVERVADDGTQTPLNHVL
                     PGIDTLIIISFDSLRTGQAADAAEVTAARAFLDDPDHLVFVCPHHEIGAAEHLSDDER
                     LKLQEAEFFHHGDRTIPPRQRFGGLARSLLAGLGIPVENHFGLRPAAAQDGSPAPIEI
                     ESSLDRLKILDGVPTFNLHPHLPHLERLGEAQVKLDVLARQHIDLTAPPHAFVQSGHS
                     TFDAMLQSSPETFPGMLLVGDSTLFSSTAGGLESLRHLWTNIVARPRAI"
BASE COUNT          330 a          439 c          499 g          329 t
ORIGIN      
        1 caaatcaccg tcgtcttctg cgaaacgtcc aagccaacaa attgggtcat cgatcagcct
       61 ccggatgcgc gaatcaccgg gcgtcagcgc ccggatcgcg gcactatcgg tagatcatga
      121 gccgaccgcg attaccggat gtccacacac ggcccagaag ccgatattcg acctgagcca
      181 tgaggccggg tttgggctat ctcgaagaaa ctgatgagat tatatctgct tagaattttt
      241 gcttgaggtc caggccatgg ccagttggcc ggtgcaagat gccaagatgc gttttggtga
      301 actcgtcgag cgcgcgccga ggaaatgcgg agcccgtagc gtgatctcaa atcgcgcgcg
      361 gccgcgcgac gatattggtc cacaaatgcc gaaggctttc gagccctccc gccgttgagc
      421 tgaaaagggt tgaatctccg acgagaagca ttcccggaaa agtctcgggg ctggattgca
      481 gcatcgcgtc aaatgtcgaa tgcccacttt ggacaaaagc atgaggtggg gctgtcagat
      541 cgatatgttg gcgcgcgagc acgtcgagtt ttacctgggc ctcgccgagg cgttcgagat
      601 gcggcaaatg cgggtgaagg ttgaaagtcg ggacgccgtc caggatcttc aggcggtcaa
      661 gtgagctctc gatctctatc ggtgccggcg acccatcttg cgccgcggcg ggccgtagcc
      721 cgaaatgatt ttcgaccgga attcccaagc ctgcgagcag cgagcgcgct aatccgccaa
      781 accgttgccg cggcggaatg gtccgatctc cgtggtggaa aaattccgcc tcttgaagct
      841 tgagccgctc gtcgtcggaa agatgttcgg cggcgccgat ctcgtgatgc ggacagacga
      901 agacgagatg gtcaggatcg tcgaggaagg cgcgtgcggc agtcacttcg gcagcatcgg
      961 ccgcctgtcc cgttcgcaaa gagtcgaaac tgatgatgat gagcgtgtcg attccgggca
     1021 ggacgtgatt tagcggagtt tgcgtgccat cgtcggcaac acgttcgact tcgagaaccg
     1081 gatggccggt ctgggtgctc gcttgttcgg caaagtcgat gaagtttgcc ttcatgatgt
     1141 gatccagaaa gccggcgatg ctctggtcaa agcgaaccgg atccgacagc tcatgaaatc
     1201 gcgggtaaac gagccggcgg ctttcgaaaa gcgagggaaa acgatcttcg atgacggcaa
     1261 gaggtgcctc aatctctcct gggcggctcc aggcatagta gacagcgact ttccgctcat
     1321 tcatgtccga gccccttctt cgcgccgcga caggttagtg cgggccaccg tctgctgcat
     1381 ttagaatgta taacgcgtgc gatgtaagac taattttgca cctgctggag cctcatcgtg
     1441 ccgcggttat ccgaagacga gttgatcgcg acaatcttcg cccctctggc gggccccggc
     1501 ggcttgcggc ttgaggacga tgccgcgctg gtggcgtgcg cgccaggcga ggaactcgtc
     1561 ctgaccaagg ctatgctcgt cgcgggcgtg catttct
//
LOCUS       DASWOG010000658         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_273753,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000658 DASWOG010000000
VERSION     DASWOG010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273753"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGB93_13485"
     CDS             <1..293
                     /locus_tag="VGB93_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147129.1"
                     /translation="GAYAAQTTRFRCAVGKVMLCNEGANLPCGKANLSKHLPGATAWC
                     KDNPTTGFIPMAATGHDTIYDWRCVTGVATAGAPIRKIDARGFFADYWRPLP"
     gene            369..794
                     /locus_tag="VGB93_13490"
     CDS             369..794
                     /locus_tag="VGB93_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEY0147130.1"
                     /translation="MSILGSIVSAIFSHGKAPATPASTSTPAAPSASSAPAATPAPET
                     PAPAPTSAAPSAPVDLDAVLMQLAAAHHERLDWKHSIVDLLKVLNLDSSLAARKELAT
                     ELHYSGDEHDTAAMNIWLHKQVIAELVKNGGKMPSDLQH"
     gene            complement(1427..2113)
                     /locus_tag="VGB93_13495"
     CDS             complement(1427..2113)
                     /locus_tag="VGB93_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY0147131.1"
                     /translation="MTGHEIRPGDGAVERLAFIRERIARAEADAGRAPGSVTLVCVSK
                     TFEAAAIRPVIAAGQRVFGENRVQEAMGKWPALRNETLGLELHLLGPLQSNKAREAVA
                     FFDVIESVDREKIAVALANEIENAARAPKLYVQVNTGAEPQKAGILPEQADAFLAFCR
                     NDLKLEISGLMCIPPVDEQASPHFALLNKIAARNGSAILSMGMSADFEIGIELGATHV
                     RVGSAIFGAR"
BASE COUNT          485 a          733 c          665 g          452 t
ORIGIN      
        1 gcggcgctta tgcggcgcag acgacgcgct tccgctgcgc cgttggaaag gtgatgcttt
       61 gcaacgaagg cgcgaatctg ccgtgcggca aagccaatct gagcaagcac ctgccgggcg
      121 cgacggcctg gtgtaaggat aatccgacga cgggttttat tccgatggcc gcgactggcc
      181 atgacacgat ctacgattgg cgctgcgtaa ctggcgtggc gaccgctggc gcgccaatca
      241 gaaaaatcga tgcgcgcggt ttcttcgccg attattggcg accgctcccg tgaacctccg
      301 cacaggccgg gagcaattcg cctgtcacat tgggcgccca atatggatcg cacagcagga
      361 ggaatttgat gagtattctc ggcagcatcg tttcagcgat tttcagccac ggcaaagctc
      421 cggccacgcc agcctcgact tcgacgccgg ctgccccgtc agcgtcctcg gcgcccgcgg
      481 ctacccctgc gcccgaaacc ccggctcctg cgccgaccag cgccgcgcca agtgccccgg
      541 ttgatttgga tgcggtgttg atgcagcttg ccgcggctca tcatgagcgg ctcgactgga
      601 aacattcgat cgtcgatctt ctgaaagtgc tgaacctcga cagcagcctc gcggcccgca
      661 aggaactcgc gacggaattg cattattcgg gtgacgagca cgacacggcg gcgatgaaca
      721 tctggctgca caaacaggtc atcgctgaac tcgtcaagaa cggcggcaag atgccgtcgg
      781 atttgcagca ctgaactgct tctgtgctct cacgcccaag aatgatgcga tttctaacga
      841 aacaacgctc cgaccgccta cgcaaattcg ctaaacgctg ggaatgattc gactcccagt
      901 agcagcggag tcagagtcgc aacctgtgaa gtgattcggg cggggcggtt cagacgccgc
      961 ccgcgtcaat cccgtcgaac cggtgtcgct tacgggactg agtctgcgac tgggagtcat
     1021 catctcccaa tggctctggg tcaaggcggc ccttctctca cggggagcgg ctgtataccg
     1081 cgaaacgtat ggcttggtat aaatacccgg aacatctgaa gaaatccaac ggcgacgcct
     1141 acgataaaga atacaaaccc ggtgccagat cattctggaa tctattggtg caaaggttgt
     1201 ggtcgcgaag tcgttgcgga acaatctcgc aaattgcctc cacaagacca tcaccaacac
     1261 ccccctgcgc aaggcgatat tcgttggtat ctgatcgtct atgcggatca tgagaggcgg
     1321 aactagaagg cctcgccagt cgcaagcttt gcatcgattg atcgtcggag acaaggactg
     1381 tcccgtgtag gatcggcgcc acgctctttg atgcgcgcgt aaagttttat ctggccccga
     1441 aaatagcgct gccgacacgc acatgcgtcg cgccaagctc gatgccgatt tcgaaatcgg
     1501 cactcatgcc catcgataaa atggcgctcc cgttacgggc ggcgatcttg ttcaacagcg
     1561 caaaatgcgg cgatgcctgc tcatcgacag gcggtatgca catcaggccg gaaatttcga
     1621 gcttgagatc gttgcggcag aaggcaagga acgcatccgc ctgctcgggc aggatacccg
     1681 ccttttgcgg ctctgcaccc gtattcacct gcacatagag tttcggcgcg cgcgccgcgt
     1741 tttcaatttc gttcgccaac gcgaccgcga tcttttcccg atcgacgctt tcgatcacgt
     1801 cgaagaacgc gaccgcttcg cgcgccttgt tcgattgcaa aggtccgagg agatgcagct
     1861 cgaggccgag cgtctcgttc cgtagcgccg gccatttgcc catggcttcc tgcacgcggt
     1921 tttcgccgaa aacgcgctgg ccggccgcga tgaccggccg gatcgcggcg gcctcgaagg
     1981 tcttcgagac gcagacgagc gtcaccgaac ccggcgcacg gccggcatcg gcttcggccc
     2041 gggcgatgcg ctcgcgaata aacgcaagcc gctcgacggc gccgtccccc ggccggattt
     2101 catgtcccgt cacaaccagc ctccttcgcg ccccgcatca ttgacccgcc ttgccaataa
     2161 tgctgtgtat ccgccgccca atcttaagat tttcaatcgc atcctggccc gaaaacccgc
     2221 ttcgcacgtt ccggcccgat gccctagacc ctgatagcat ggacattgcg cgctacaacg
     2281 ctcgcgaagc cgagcaaaaa tggcaacaga cctgggacgc ggcggagacc ttccg
//
LOCUS       DASWOG010000659         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_310884,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000659 DASWOG010000000
VERSION     DASWOG010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310884"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VGB93_13500"
     CDS             complement(<1..730)
                     /locus_tag="VGB93_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0147132.1"
                     /translation="MAAQLSPGDTIGILGGGQLGRMLAMAAARLGLKTHIFCPETDSP
                     AFDVAAAKTIALYENESALQAFAEAVDVVTYEFENVPARTADLLAALRPVRPGPAALA
                     VSQDRLTEKDFLTDLGIATAPYRNVEDAGALARAVGELGRPSILKTRRFGYDGKGQVM
                     IREGSDLGAVFRGLGGAPSILEGFVPFIKEISVIAARGLDGAFAAYDVTENVHTNHVL
                     SVSRAPARVDPACATAAIAMAPKIA"
     gene            complement(735..1220)
                     /gene="purE"
                     /locus_tag="VGB93_13505"
     CDS             complement(735..1220)
                     /gene="purE"
                     /locus_tag="VGB93_13505"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY0147133.1"
                     /translation="MSTPKLVAIVMGSQSDWKTMRHAAETLDKLDVSYSAEIVSAHRT
                     PDRLVKFAKGAEALGFKVIIAGAGGAAHLPGMTAAMTPLPVLGVPIESKALSGQDSLY
                     SIVQMPAGIPVGCLAIGEAGAVNAALLAAAILALDDKALAGRLADWRAAQTAAVAKHP
                     E"
     gene            complement(1329..2111)
                     /locus_tag="VGB93_13510"
     CDS             complement(1329..2111)
                     /locus_tag="VGB93_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147134.1"
                     /translation="MAENASKTVVFIHGAWMIPACWDNFRRSFQAAGYTTHAPAWPYL
                     DSASSDELRRDPPPGLGALGITDIVDHYQTFIKAMPEKPLIIGYSFGGLFTQLLLDRG
                     LGWAGVAIDPAPIAGVVPGAVSLAAASPVLLRWQGWEQPFQLSREAFARSFANAAPPD
                     IQNEAYDRLVVPAPGRIFYEDAFWIGTHVHPKRRTQPLLITAAEKDRTVTPFTAMGAF
                     NLQKKSSAETDFKYFPGHSHFLIGEPGWEDVANYILRWVAHL"
     gene            2317..3189
                     /locus_tag="VGB93_13515"
     CDS             2317..3189
                     /locus_tag="VGB93_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147135.1"
                     /translation="MSAELSAASAGQFEIGGDFTINRFGFGAMRLTGKSIWGDPANPA
                     AARETLRLLPDLGINFIDTADAYGPFVSEDLIREVLHPYKNMMIATKGGLARPGPETW
                     RPLGRPEYLRQCVMMSLRRLGVARIDLWQLHRIDPSVPRDEQYSAIAAMRKEGLIRHV
                     GLCDVGIDDIEAAHKYFPLATVQNRFNLVNRSSEDVLIYCEQHNIGFIPWAPLAAGAL
                     AHPGSALMQTARTLKATPSQVALAWVLKRSKIMLPIPGTANPEHLVENVAAATLELSD
                     EAFAALDQQGRNAL"
     gene            3272..4954
                     /locus_tag="VGB93_13520"
     CDS             3272..4954
                     /locus_tag="VGB93_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEY0147136.1"
                     /translation="MDATLSLLDLAGAVALLLWAMHMIQSGIQRAFGADLRRVLAKAF
                     GNRAKAFAAGLGVTAVLQSSTATGLMVAGFAAAGLVDLVPALAVMLGANVGTTLIVQI
                     LSFDIALAAPIFILIGVMMFRRSGGQARTRDIGRAIIGLGLLLMSLSQLLQIATPYED
                     TPSLRMLLGAIATQPLIALLFGALATWAAHSSVAIVLLVMSFVTNGAISLDMGIALVL
                     GANIGSALNPVLEAPRGVDRTGAQVAVGNLLNRIIGAGIALPLIDWIGPNLVRLDPSL
                     ARDVANFHMFFNIAMALIFMPLLPLFAKALRRLMPQRIEAQDPSRPLYLDPSAVEAPA
                     VAIGNATREALRMVDVLDEMLQGLGRLLAEQTDKQRIVETRRIDDILDRLNTAIRSYL
                     VGLDPESLTEDDDKRVAAILTFTSHLEQAGDTIDRSALATLTRQLKRGLVLADQGRAD
                     ARLMLEHLTADLRLAATVFMTQDVRAARTLADEKGFFRDIESKATAAYFRRLRESRPD
                     TIEANALHLDLLRALKSVNDSLVAGAAYPVLEERGELHASRLRAQPDGDSEG"
BASE COUNT          908 a         1541 c         1589 g          938 t
ORIGIN      
        1 cggcgatctt tggcgccatt gcgatcgccg ccgtggcgca ggccggatcg acccgcgccg
       61 gggcgcggct gacgctcaga acatggttgg tatggacatt ctcggtcacg tcataggcgg
      121 caaaggcgcc gtcgaggccg cgtgccgcga tgaccgagat ttccttgatg aacggaacga
      181 agccttcgag gatggacggg gcaccgccaa ggccgcgaaa caccgcgccg aggtcggaac
      241 cctcgcgaat catcacctgg cccttgccgt cgtagccgaa tcggcgtgtc ttcaggatcg
      301 aggggcggcc gagctcgccg acggcgcggg ccaaggcgcc ggcatcctcg acattgcgat
      361 agggcgccgt ggcgatgccg agatcggtca gaaaatcctt ctcggtgagc cggtcctggc
      421 tcaccgcgag cgcggccggg ccgggccgca cgggccgcag cgcggcgagc aagtcggcgg
      481 tgcgcgcggg cacgttttcg aattcgtagg tgacgacatc gacggcttcg gcaaaggctt
      541 gcagcgccga ctcgttttcg taaagcgcga tggtcttggc cgccgcgaca tcgaaggccg
      601 ggctgtcggt ctcggggcag aaaatatggg ttttgaggcc gagccgcgcc gctgccatag
      661 ccagcatccg gcccaattgg ccgccgccta aaattccgat tgtgtcgccc ggtgagagct
      721 gcgcggccat caacctactc gggatgcttg gcaacagcgg cggtctgtgc cgcgcgccaa
      781 tctgcgagcc gcccggcgag cgccttgtcg tcgagcgcga ggatcgccgc ggcaagcagg
      841 gccgcattga cggcgccggc ctcgccgatc gccaggcagc cgaccggaat gcccgccggc
      901 atctggacga tggaataaag cgaatcttgg cccgaaagcg ccttgctctc gatcggcacg
      961 ccaagaacgg gtagaggggt catggctgca gtcatgcccg gcagatgcgc agcgccgccg
     1021 gccccggcga tgatcacctt gaaccccaac gcctccgcgc ctttggcgaa tttgaccagc
     1081 cggtccggcg tgcgatgcgc cgacacgatc tccgccgaat agctgacatc aagcttgtcc
     1141 agcgtttccg cggcgtggcg catggtcttc caatcggact ggctgcccat gacgatggcg
     1201 acaagcttgg gagtggacat gaagaccttg caaggcggct gaacagccgg ccgaaaagaa
     1261 aggcagcgtc ctagacgaag ggccgttgcg gcgcaagcgg cgcagtcgct taactgccaa
     1321 tagtttgctc agagatgggc gacccagcgc aggatataat tcgcgacgtc ttcccagccg
     1381 ggctcgccga tcagaaaatg cgaatgcccg gggaaatatt tgaagtccgt ctcggccgac
     1441 gactttttct gcaagttgaa ggcgcccatc gcggtgaacg gtgtcaccgt gcgatctttt
     1501 tcggcggccg taatgagcag cggctgcgtg cggcgcttgg gatggacatg cgtgccgatc
     1561 cagaacgcgt cttcgtaaaa aatgcgcccc ggcgcgggaa cgacgagccg gtcgtaagct
     1621 tcgttctgaa tatccggcgg cgccgcgttg gcgaacgacc gggcaaaagc ttcacgcgac
     1681 agttggaacg gctgctccca gccttgccag cgcaagagca caggcgacgc ggcggcgagc
     1741 gagacagcac ccggaaccac gccggcaatc ggtgccggat cgattgcaac accggcccag
     1801 cccaagccgc gatcaagcag aagttgggtg aaaagaccgc cgaaggaata accgatgatc
     1861 agcggcttct ccggcatggc tttgatgaac gtctgatagt gatcgacgat atcggtgatg
     1921 cccagcgcac cgaggccggg cgggggatcg cggcgtaatt cgtccgatga tgcactgtcg
     1981 agataaggcc atgccggcgc gtgggtcgtg taaccggcgg cctgaaagga tctgcggaaa
     2041 ttgtcccagc aggccgggat catccaggca ccatggatga agacaacagt tttgctggcg
     2101 ttctcggcca tgatcgaacg tcctgtcctg gcgctcctgc accgtcacat cgccgcaaaa
     2161 agaagctgcg cctatggcgt gacgaatgca agacgtcgcg aagacatcaa gcgagacatg
     2221 gcggaggttg ccgtgcatct ggttttcaag cccgaagccg ggctagaaat tgctgcggtc
     2281 acggcggcgc gtaccgcgca acacatggaa acagccatgt cagcagagct ttccgctgcc
     2341 agcgcaggcc aatttgaaat tggcggcgat ttcacaatca atcgcttcgg ctttggcgcc
     2401 atgcggctga ccggcaaaag catctggggc gatccggcaa accccgccgc cgcgcgcgag
     2461 actttgcgtc ttctgccgga tctcggcatc aatttcatcg atacggcgga tgcctatggc
     2521 cccttcgtca gcgaggatct catccgcgag gtcctgcatc cttacaaaaa catgatgatc
     2581 gcgacgaaag gcggcctcgc ccggccgggc ccggagacgt ggcggccgtt ggggcggccg
     2641 gaatatttgc gtcaatgcgt gatgatgagc ctgcgccggc ttggcgtcgc gcgtatcgat
     2701 ctctggcaat tgcaccggat cgatccatca gtgccgcgtg acgagcaata ttcggccatc
     2761 gccgcgatgc gtaaggaagg cctgatccgt catgtcgggc tttgcgatgt cggcatcgat
     2821 gacatcgagg cggcgcacaa atattttcct cttgcgacgg ttcagaaccg gttcaatctc
     2881 gtcaatcgct cgagcgagga tgtgctgatt tattgcgagc agcacaatat cggcttcatc
     2941 ccatgggcgc cactcgctgc gggtgcgctg gcgcatccgg gctcggcctt gatgcagacg
     3001 gcaagaacgc tcaaggcgac gccaagccag gtcgcgctgg cgtgggtgct gaagcgctcg
     3061 aagatcatgc tgccgattcc cggcacggcc aatcccgagc atcttgtcga gaatgtcgcc
     3121 gccgcgacgc tcgaactctc ggacgaggct ttcgccgcgc tcgatcagca ggggcgcaac
     3181 gcgctctgaa tcgaagcttg atattcgtgc gttttatacc accttgtgca aacgctccgt
     3241 cgcactcggc gcgcggctga aaggaacaaa gttggacgcc acgctttctc tgctcgatct
     3301 cgccggtgcc gtcgcgcttc tgctctgggc gatgcatatg attcagagcg gaattcagcg
     3361 tgccttcggt gccgatctgc gccgcgtact tgcaaaggcg ttcggcaata gggcgaaggc
     3421 cttcgcggct ggcctcgggg ttaccgcggt tctccaaagc agtacggcga cgggcctgat
     3481 ggtggcgggt tttgccgccg ccggcctcgt cgatctcgtg ccggcgctgg ccgtcatgct
     3541 cggcgccaat gtcggcacga cgctgatcgt ccaaattctg tcgttcgata tcgcgctcgc
     3601 cgcgccgatc ttcatcctga ttggcgtcat gatgtttcgc cgcagcggcg gtcaggcacg
     3661 cacgcgtgac attggccgcg ccatcatcgg tctcggcctg ttgctgatgt cgctgagtca
     3721 attgttgcag atcgcgacac cttacgagga tacgccaagc ctgcggatgt tgctcggcgc
     3781 gatcgcgaca cagcctctca ttgctttgct ctttggtgcg ctggcgacct gggcagcgca
     3841 ttcgagcgtc gccattgtcc tgctcgtcat gtccttcgtc accaatggcg ccatctcgct
     3901 tgacatgggc attgcgctcg tgcttggcgc caatatcggc tcggccctca atcccgtgct
     3961 ggaggcgccg cgcggcgtcg atcgcacggg tgcgcaggtt gctgtcggca atttgctcaa
     4021 tcggatcatc ggtgctggga tcgctctgcc gctgatcgac tggatcggcc ccaatctcgt
     4081 gcggctcgac ccgagcctcg cgcgtgatgt cgccaatttt catatgttct tcaatatcgc
     4141 gatggcgctc atcttcatgc cgttgttgcc gctcttcgcc aaagcgctgc gtcgcctcat
     4201 gccgcagcgg atcgaggcgc aggatccctc gcgcccgctc tatctcgatc cctcggcagt
     4261 ggaggcgccc gcggtcgcga tcggcaatgc gacgcgcgag gctctgcgca tggtcgatgt
     4321 gcttgacgaa atgctgcaag gccttggccg tctgctagca gagcagaccg acaagcagcg
     4381 aatcgtcgag acgcggcgga tcgatgacat tctcgaccgg ctcaacaccg cgatccgctc
     4441 ctatctcgtc ggcctcgatc cggaatcctt gacagaggat gacgataagc gggtcgcggc
     4501 catccttacc ttcacgagtc atttggagca ggccggcgac actatcgacc gcagcgcgct
     4561 cgcgacgttg acgcggcagt tgaagcgcgg gcttgttctg gctgatcaag gtcgtgccga
     4621 cgcacggctc atgcttgaac atctcaccgc cgatctgcgc ctcgccgcaa ccgtgttcat
     4681 gacacaagat gtgcgcgcgg cgcggaccct ggcggatgaa aaaggcttct tccgtgacat
     4741 cgagtcgaag gcgacagcgg cttacttccg ccgcctgcgt gaatcgcggc cagatacgat
     4801 cgaggccaac gccttgcatc tggatttgtt gcgcgcattg aagagcgtca acgactccct
     4861 cgtggccggc gccgcctatc cggtcctcga agagcgtggc gagttgcacg cctcgcgctt
     4921 gcgcgcgcag ccggatggcg actccgaagg ctaatcagcc gacgcggcgg gcgatc
//
LOCUS       DASWOG010000660         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_16872,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000660 DASWOG010000000
VERSION     DASWOG010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16872"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2528
                     /locus_tag="VGB93_13525"
     CDS             <1..>2528
                     /locus_tag="VGB93_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0147137.1"
                     /translation="YWVSTVEAMPRDLNLSFVAVDEIQIAANLDRGHVFTDRLLNRRG
                     LDETLLIGASTMQRPLEALLPGAPIISRPRFSKLSFAGERKIARLPPRSAIVAFSVEE
                     VYAIAEWIRRQRGGAAVVLGALSPRTRNAQVEIYQSGDVDYIVATDAIGMGLNLDVDH
                     VAFAADRKFDGWQFRKLNPAEFGQIAGRAGRHMRDGTFGTTGRCAPFDNELVEAIEEH
                     RFEPVTMLQWRNSDLDFASIAELTASLDQYPTDRGLARAPLAEDQMVLDIAARDENVL
                     RATKTAADVLRLWDACQIPDYRKLSPAAHAELVLSIYGFIVRARHIPEDWFSRHIQEL
                     DRVDGDLDTLSARLAQVRTWTFIANRPDWLREPEHWQEVARQVEDRLSDALHERLAQR
                     FIDRRTSVLMRRLRENAMLEAEITPGGDVLVEEQHVGHLSGFRFTADPLADGEAAKAL
                     NAAAQKALASEIEGRARRVHEAVDEAFVLANDGVIRWLGEAIGKIVPGEHILQPRVTV
                     LADEQLTGASLEIVQKRLDLWLAQHVKKLLGPLAELENGQGLEGIARGVAFRISESLG
                     VLERSQVAEDVKSLTQEARATLRKLGVRFGAYHLYLPALLKPAPRAFAAQLWALRNST
                     VESIQGQAEVPHLAASGRTSFVADKDIPKGFYRAAGFHVCGERAVRVDILERLADLIR
                     PAIAYRPGTTPGDPPAGAADGDGFIVTVSMTSLAGCSGDAFASILRSLGYQSQQRNGP
                     AITVPLVPKASIEPITPATVEAASQDATSTDPAASSVESGSNDGASTLDVVSDEPPAA
                     PDAVSDEATVAAEPATVEVIEVEVIDPVIAELAEAEAQVAVAVA"
BASE COUNT          444 a          730 c          829 g          525 t
ORIGIN      
        1 gctactgggt ttcgacggtc gaggcgatgc cgcgcgatct caatcttagc tttgtcgccg
       61 tcgatgagat ccagattgct gccaatctcg accgcggcca tgtcttcacc gaccggctgc
      121 tcaatcgtcg tggccttgac gagactttgc tgattggcgc ttcgacaatg cagcgcccgc
      181 ttgaggctct gttgcccggc gcgcctatta tctcgcggcc gcgattttcg aagctgagct
      241 tcgcagggga gcgaaaaatc gcgcgcctgc cgccgcgcag tgcgattgtc gctttctccg
      301 tcgaggaagt ctatgcgatc gcggaatgga tcaggcgtca gcgcggcggc gcggcggttg
      361 tcctcggtgc tttgtcgccg cgcacacgca atgcgcaagt cgagatttat caaagcggcg
      421 atgtggatta tatcgttgcg accgacgcta tcggcatggg tcttaatctt gatgtcgatc
      481 acgttgcgtt cgcggcggat cgcaaattcg atggctggca atttcggaag ctcaatccgg
      541 ccgaattcgg ccagattgcc ggtcgtgccg ggcgccacat gcgcgacggc acgtttggta
      601 cgaccggacg ttgcgcgccg ttcgacaacg aacttgtcga agcgatcgag gaacatcgtt
      661 tcgagccagt cacaatgttg caatggcgca atagcgacct cgactttgcg tcgatcgctg
      721 agctcacagc gtcgcttgat cagtatccga cggatcgggg tctggcgcgc gccccgctcg
      781 cggaagatca aatggtgctt gacattgcgg cgcgggacga gaatgtcctg cgggcgacca
      841 agaccgcggc tgacgtgctg cggctttggg acgcttgcca aattcccgac taccgcaagc
      901 tctcgccggc ggcgcatgcc gaactcgtac tttcgatcta tggctttatc gtccgggcgc
      961 gtcatattcc cgaggattgg ttttcacgcc atatacagga acttgatcgc gtcgatggcg
     1021 accttgacac cttgtcggcg cggctggcgc aggtcaggac atggacattc atcgccaacc
     1081 ggcctgattg gcttcgtgag ccagagcatt ggcaggaggt cgcgcgtcag gtagaggaca
     1141 ggctgtcgga cgcgttgcac gaacgtctcg cacaaagatt cattgaccgg cgcacaagcg
     1201 tgctgatgcg ccgtctgaga gagaacgcga tgttggaagc cgaaatcacc ccaggcggcg
     1261 atgtgctggt tgaagaacag catgtcgggc atttgtcggg atttcgtttt acggccgatc
     1321 cgctggccga tggcgaagct gccaaggcct tgaatgccgc ggcgcaaaaa gcgttggcga
     1381 gcgagatcga gggccgcgcg cgccgcgtgc atgaagcggt cgatgaggct ttcgtcctcg
     1441 ccaacgacgg cgtcatccgc tggctcggcg aagcgatcgg caagattgtg ccgggcgagc
     1501 atattttgca gccgcgcgtc actgtcctcg ccgacgaaca attgacgggc gcgtctttgg
     1561 aaattgtcca gaagcgtctc gatttgtggc tggcgcagca cgtcaaaaag ctgctgggtc
     1621 cgcttgccga attggaaaat gggcaggggc ttgagggcat cgcgcgcggc gtggccttcc
     1681 gtatttctga atcgctgggc gtgcttgaac gttcgcaagt tgcggaagat gtgaaaagtc
     1741 tcacgcagga agcgcgcgcg acattgcgca agctcggcgt gcggttcggg gcctatcatc
     1801 tttatctgcc ggcgctgttg aagccggcgc cgcgcgcctt cgccgcgcag ctttgggcct
     1861 tgcgcaattc tactgttgaa agcatccagg ggcaggccga agtgccgcat cttgccgcct
     1921 cgggccgtac gtccttcgtt gccgacaaag acattccgaa gggattttat cgcgcggccg
     1981 gtttccatgt ctgcggcgag cgggccgtgc gggtcgatat attggagcgg cttgccgatc
     2041 tcattcgccc ggcgattgcg tatcggccgg gcaccacgcc gggggatccg ccggcaggtg
     2101 ctgcggatgg cgacggcttc attgtgaccg tgtcgatgac gtcgctcgcc ggatgctctg
     2161 gcgatgcttt cgcctcgatc ctgcgttcgc tgggctatca aagccaacag cgcaacggcc
     2221 cggcgattac cgtgccgctg gttccgaaag cttcgatcga gcccatcacg cccgcgaccg
     2281 tggaggcggc gtcgcaagac gcgacttcga cggacccagc cgcgtcaagc gtcgaaagcg
     2341 gttcgaacga cggggcttcg acgctggacg tcgtttcgga tgagccgccg gcagcgcccg
     2401 acgccgtttc ggacgaggcg actgtcgcgg ctgaacccgc cacggtcgag gtgattgagg
     2461 ttgaggtcat tgaccccgtt attgccgaac tggctgaggc agaggcgcag gtcgcagtgg
     2521 ctgtagcg
//
LOCUS       DASWOG010000661         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_328818,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000661 DASWOG010000000
VERSION     DASWOG010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328818"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VGB93_13530"
     CDS             <1..900
                     /locus_tag="VGB93_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEY0147138.1"
                     /translation="LAREVSGKDTVLTKLNKQIAELTAQLALSQMNKSNADNQINVLS
                     ATLDSERKKSAALQGEIAAASAGNGSADARVTAAEQALKAEKDVSAQALAQVEILNQQ
                     IGALRRQLAAIEDALAASESRDKESQAKIADLGSRLNIALAQKVQELARYRSDFFGRL
                     RQILGDRPDIRTVGDRFVFQSEVLFDTGKAELNPEGKTDLDKLADAVVELNKEIPPEI
                     PWVLRVDGHTDKRPIQSTEFHSNWDLSASRAIAVVEYLISKGVPADRLVAAGFGEFQP
                     IDPGESDDAYAKNRRIELKLTEK"
     gene            1040..1369
                     /locus_tag="VGB93_13535"
     CDS             1040..1369
                     /locus_tag="VGB93_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147139.1"
                     /translation="MAGSALILLGSVLVLTSAALIPHQPIFVFGLETFLIALTMLLVP
                     LLIQVRSCKIEPMPRRRTIIRAIMNGAPGLAILIAGVLLLLGQASGLYWIAAGAILSL
                     ISGVMIA"
     gene            1457..1533
                     /locus_tag="VGB93_13540"
     tRNA            1457..1533
                     /locus_tag="VGB93_13540"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1491..1493,aa:Ile,seq:cat)
     gene            complement(1512..2654)
                     /locus_tag="VGB93_13545"
     CDS             complement(1512..2654)
                     /locus_tag="VGB93_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0147140.1"
                     /translation="MTELQLAMGQQFGWTARFLVRLREDRVYFSIIGVFFALTAGLDR
                     LTDHSYLLETTYAYLNFWMAVALTMFAAALSMQFVSRAVRDRHERPFELLYDVLKFIF
                     TPETVAGLILSAALTVFMGAFTAVKSELPILNDFWADPWLARADLIVNFGHDPWSLLQ
                     PVLGQHWITRAIEFVYAPVWTASVIFVPFLFCVTTRMPAHRNRFIMAYLLTWIVSGVI
                     LAGVFMSGGPAFYGELTHDQARYGELVRYLRFDMGTPFSAAWDQRLLWKLHSENSATV
                     GAGISAFPSLHVSMILLCCLGVWQVSRRFACVFLAIAAVIVLGSVHLGWHYSVDTYAA
                     ILITLLIWKAADHVTSGQGENPWLRHQFIENRTESDANWIGGPGRT"
BASE COUNT          712 a          876 c          838 g          575 t
ORIGIN      
        1 ctcgcgcgcg aagtctcggg caaagatacg gtgttgacca agctcaacaa gcagatcgcc
       61 gaactgacgg cgcagctcgc cttgtcgcag atgaataagt ccaacgccga caaccagatc
      121 aacgtgctga gcgcaaccct ggacagcgag cggaagaaaa gtgccgccct acaaggcgag
      181 atcgccgcgg ccagcgccgg aaacggcagc gccgatgcgc gtgtcaccgc cgccgaacaa
      241 gctttgaagg ctgaaaaaga cgtgtcggcg caggcactgg cgcaggtcga aattctcaat
      301 cagcaaattg gcgcgctgcg ccgccagctc gcggcgatcg aggacgcgct cgcggcctcc
      361 gagtctcgcg ataaggaatc gcaggccaag atcgctgacc ttggctcgcg gctcaacatt
      421 gcgctcgcgc agaaagtgca ggagcttgcg cgctatcggt ccgacttctt cgggcgctta
      481 cgccaaatcc ttggcgaccg gccggatatc cgcaccgtcg gcgatcgttt cgtcttccag
      541 tcggaagtcc tgttcgacac cggcaaggct gagctcaatc ccgaaggcaa gaccgatctc
      601 gacaagcttg ccgacgccgt cgttgaactc aacaaggaaa tcccgccgga aattccgtgg
      661 gttttgcgcg tcgatgggca caccgacaaa cgcccaattc aatcgaccga attccattcc
      721 aactgggatt tgtcggcgtc acgcgccatt gcagttgtgg aatatctcat cagcaagggt
      781 gtgccggccg accggcttgt cgccgcgggc ttcggcgaat tccagccaat cgatcccggc
      841 gaaagcgatg acgcctacgc caagaaccgc cgcatcgaat tgaagctgac ggaaaaataa
      901 agccggcgcg cgtgaacgaa attcagacgg ccgattggag caattttttc gtcgcggagg
      961 tcggcgcctc ggcggccctc accggccttg ttgccgttgc gatctcgagc gcatccttgc
     1021 cttcaaaact ctgccaggat tggctggctc cgctttgatt ttgctcggca gcgtcctcgt
     1081 ccttaccagc gcggcgttga tcccgcacca gccaatattt gtcttcggcc tcgaaacttt
     1141 cctgatcgct ctcaccatgc tgctcgtgcc gctgctcatc caggtccgct cgtgcaagat
     1201 cgagccaatg ccgcggcggc ggaccatcat tcgcgcgatc atgaacgggg cgccgggcct
     1261 tgcgatcttg attgccggcg tcctgctgct cctcggccag gcgagcgggc tttactggat
     1321 cgccgccggt gccattttga gtcttatttc gggcgtgatg atcgcctgag ttctgttgat
     1381 cgaaatcctc cgctaagcgc ggcttgcgcc tttgcccgag ttcagtgtaa tgcccgcagc
     1441 cttgccgcat gctgccgggc cggtagctca attggttaga gccggccgct cataacggtc
     1501 tggttgcagg ttcaagtcct gcccggccca ccaatccaat tggcgtcgct ttccgttcga
     1561 ttctcgatga attgatgccg gagccaagga ttctcaccct ggcccgacgt gacatggtca
     1621 gcggccttcc aaatcaacag cgtgatcaga attgcggcat aagtgtcgac ggaataatgc
     1681 catccgagat gcaccgaacc gagaacgatg acagcagcaa tcgcgaggaa aacacaggcg
     1741 aagcgacggc tgacctgcca tacgcccaga cagcaaagca agatcatact gacgtgcagg
     1801 ctcggaaagg cagaaattcc agcgccgacc gtggcggagt tttcgctatg caacttccac
     1861 aacagacgct gatcccatgc ggcagagaat ggcgtcccca tatcaaaacg gagatagcgc
     1921 acgagttcgc catagcgcgc ctgatcgtgc gtaagttcgc cataaaatgc cggaccgcca
     1981 gacatgaaca cccccgccaa aatcacgcca ctgacaatcc atgtcagcaa ataggccatg
     2041 atgaagcggt tgcgatgcgc cggcatacgc gtcgttacgc agaagagaaa tgggacgaaa
     2101 atcacggatg ccgtccagac cggcgcgtag acgaattcga tagcgcgcgt gatccaatgc
     2161 tggccgagaa caggttgaag aagagaccaa ggatcgtgcc cgaaattaac gataagatct
     2221 gcacgcgcta gccagggatc cgcccagaaa tcattcagga tcggcaactc cgactttacg
     2281 gcggtgaaag cgcccatgaa gacagtaagc gcggccgata gaatcaggcc ggcgactgtc
     2341 tcaggcgtga agatgaattt gaggacatcg taaagcagct cgaagggacg ctcatgacgg
     2401 tcgcgcacag cccttgacac gaattgcatc gagagggccg cggcaaacat agtaagtgcc
     2461 accgccatcc agaagttcag gtaggcatag gtggtttcga gaagatagga atgatctgtt
     2521 aaccgatcca atcctgcggt cagggcaaaa aaaacgccta taatagaaaa atagactctg
     2581 tcctcgcgaa gccggaccaa aaagcgagcc gtccagccga actgctggcc cattgcaagt
     2641 tgtaattcgg tcataagccc tcctgccgat caggatggct tctcgcaaaa ttactaaagc
     2701 cacaaccgcg acgcggatat aagtgtgtat tagcgtgaaa actgtaatct cgtgctgcgg
     2761 gtttccttgc agcggcgaag acaaagctag catgcatgtt gagcgttcgc taccaaaaca
     2821 ccgcgagcaa cggcgcatga ggataatcag agcttactaa aattattatc aattaaggaa
     2881 agcacacagc gctcataccg gtctggttgc cggttcaatt tctgcccgac ccaccagaat
     2941 ttacgcgcat gtgttccagt ggtccggata catgtttggt tcggcgcctt acgcgacggt
     3001 t
//
LOCUS       DASWOG010000662         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_181533,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000662 DASWOG010000000
VERSION     DASWOG010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181533"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1072
                     /locus_tag="VGB93_13550"
     CDS             77..1072
                     /locus_tag="VGB93_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0147141.1"
                     /translation="MRRFLAALCALLGFAAPALAVDKVTFGTNWRAEAEHGGFYQALA
                     DGTYRKYGLDVTILQGGPQSNNRLLLAAGRIDFDMGANMIQAFDSVAQDVPIVAVAAI
                     FQKDPFILMSHPNAGYDHFSDLPKATAIVGNDALVTVYQWLKLTYGFSEDRVKLYTFN
                     SAPFIADKASIQQGYVTSEPYAIARQGGFTPNVFLAADFGYQSYSTTIETRRDLIDKT
                     PGLVRRFVEASIIGWYHYLYGDNPKANVLIKQDNPDITDAQLAYSIAALRKYGIVDSG
                     DAQSLGIGAITDARMKAFFAQMVAAKLFKPDLDYKKAYTLAFVDRGVGLDLRTKR"
     gene            1069..1824
                     /locus_tag="VGB93_13555"
     CDS             1069..1824
                     /locus_tag="VGB93_13555"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0147142.1"
                     /translation="MRATLVALSGIAKTFPSGMQALDGLDLAVHEGELLTLLGPSGCG
                     KSTVLRLIAGLELPSSGEVQGAVWRPGDIGFVFQEATLMPWADVSANVELPLRLQRLP
                     KAEARQRCDEVLALVGLTQFAKAFPRELSGGMRMRVSIARALASRPKLLLMDEPFAAI
                     DEIGRFQLNDDLLRLKKELATTIVFVTHSIYEAVYLATRIVVMAPRPGRIVEEMSIEQ
                     GFHQSPEPRASAAYLEDCARASKALSRAAGAVQ"
     gene            1821..2597
                     /locus_tag="VGB93_13560"
     CDS             1821..2597
                     /locus_tag="VGB93_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0147143.1"
                     /translation="MTFLRRAMPPILAFLGFLAAWEAIVRLGQIPPYVLPAPSVIGAT
                     LVADRAILLPALWVTLSITLEGLVAATLGGVLIAILFAQWRWLEQAFLPFAVILQVTP
                     IIAIAPLLLIYCAPAVAVLLCAFLVAFFPILANTTLGLASAGRNLRDLFALYGASPWQ
                     TLWRLKIPAALPLFFAGLRIGGGLALIGAIAAELTAGTQGGQSGLAFRIVEAGYRLNI
                     PRMFAALALVAVTGIAIYVALAALSHWVLCRWDERASDAA"
     gene            2659..3273
                     /locus_tag="VGB93_13565"
     CDS             2659..3273
                     /locus_tag="VGB93_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY0147144.1"
                     /translation="MTINFHKGDLPNGLSFGDSVAIDTETLGLVPYRDRLCVVQLSAG
                     DGDAHVVQIAAGQTAAPNLARLLADPAVTKLFHFARFDIAVLYRAFGIMAAPLYCTKI
                     ASKLTRTYTDRHGLKDLVRELLGVDLSKQQQSSDWAAESLSEAQLAYAASDVLHLHAL
                     RGKLDTMLARENRTELAASCFEFLPTRAKLDLAGWPDSDIFSHE"
     gene            3488..>4023
                     /locus_tag="VGB93_13570"
     CDS             3488..>4023
                     /locus_tag="VGB93_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147145.1"
                     /translation="MTDLTRAPATGTSRLDLSAGASRHAVRHARWHSIFVRYLRHFII
                     AGAALVVLIVGFLILFNPFRQLQKNLTSGAVGINGTIVTLSTPKMQGVRQNGQPFELT
                     GASGTQDILNPSVIKLVGVDAKVGLDDRTTARITALSGVYDSSRDYVWLNQNVRIKNT
                     DAGYDMFTQKAEIDFVSG"
BASE COUNT          714 a         1251 c         1195 g          863 t
ORIGIN      
        1 gaggcgcggg gcaagccctt gcgccacaac gccgtccggg cgaaacatgc ggccagagcg
       61 gaggggccaa gcagacatga gacggttttt ggcggcgcta tgcgcgcttc tcggctttgc
      121 ggcgcccgct ctcgctgtcg acaaggtgac cttcggcacg aactggcgcg ccgaggccga
      181 gcacggcggc ttctatcagg cgttggccga cggcacctat cgcaaatacg gcctcgacgt
      241 gacgatcttg caaggcggcc cgcagagcaa caatcgtctg ctgctcgctg ccgggcggat
      301 cgacttcgac atgggcgcca atatgatcca ggcgttcgat tcggttgcgc aggacgtgcc
      361 gatcgttgcc gttgcggcca tttttcagaa agacccgttc attctgatgt cgcatccgaa
      421 cgcgggctac gatcatttca gcgatctgcc caaagccacg gccatcgtcg gcaatgacgc
      481 gctcgtcacc gtctatcaat ggctgaaact gacctatggc ttcagcgaag acagagtgaa
      541 gctctatacg ttcaattcgg cgcctttcat cgccgacaag gcttcgatcc agcagggcta
      601 tgtgacatcg gagccttatg ccatcgcgcg acagggcggc ttcacgccaa acgtcttcct
      661 cgctgctgat ttcggctatc aatcctattc gacgacgatc gagacgcggc gcgatctcat
      721 cgacaaaacc cccggtcttg tccgccgctt cgtcgaggcc tcgatcattg gctggtatca
      781 ttatctctac ggcgataatc caaaagccaa tgtgctgatc aagcaggaca atcccgacat
      841 caccgacgcc caacttgcct attccatcgc tgcgctgcgc aaatatggca tcgtcgattc
      901 cggcgatgcc cagagtctcg gcattggcgc gatcactgat gcgcggatga aggccttctt
      961 cgcgcagatg gtcgccgcga aattgttcaa gccggatctc gattacaaaa aggcttacac
     1021 gctggccttc gtcgatcggg gcgtcggatt ggacttgcgc acgaagcgat gagagcgaca
     1081 ctggtcgcgc tcagcggcat cgcaaagact tttcccagcg gtatgcaggc gctcgatggg
     1141 ctcgatcttg cggtccacga aggtgaattg ctgactctgc tcgggccgtc gggatgcgga
     1201 aaatccacag tgctgcggct gatcgcaggg ctggagctgc cctcgtccgg cgaagtgcaa
     1261 ggggccgtct ggcgcccggg cgatatcggc ttcgttttcc aggaagcaac cttgatgccc
     1321 tgggccgatg tcagcgccaa tgttgaattg ccgctgcgtc tgcaaagact accgaaggcc
     1381 gaggcccgtc aacgttgcga cgaggttcta gcgctcgtcg gcctcaccca atttgccaaa
     1441 gcttttccac gcgaattgtc cggcggcatg cggatgcgcg tttcgattgc ccgcgcactc
     1501 gcgtctcggc ctaaactgct gctgatggat gagccattcg cggcgatcga cgaaattggc
     1561 cggtttcagc tcaacgatga cctgctgcga ttaaagaaag aattggcgac gaccatcgtc
     1621 ttcgtgacgc attcgatcta cgaggcggtc tatctcgcca cccgcatcgt cgtgatggca
     1681 ccgcggccgg gccgcatcgt cgaagagatg tcgatcgaac agggatttca ccaatcgccg
     1741 gaaccgcggg cgagcgcggc ctatcttgaa gattgcgccc gtgcctctaa agccctgtcg
     1801 cgcgcggcgg gggccgtgca atgacgttct tgcgtcgcgc catgccgcca atcctcgcct
     1861 tcctcggctt tcttgccgcc tgggaagcga tcgtccgtct cgggcaaatt ccgccttatg
     1921 ttctgccggc gccaagcgtc atcggggcga cgctcgtcgc tgaccgcgcg atcctgttgc
     1981 cggcgctctg ggtcactctg tcgatcacgc tcgaaggtct cgtcgcggcg actttgggcg
     2041 gcgtgctcat cgcgattttg tttgcgcaat ggcgatggct cgaacaggcc tttctgccgt
     2101 tcgccgtgat tttgcaggtg acgccgatca tcgccatcgc gccgctgctg ctgatttatt
     2161 gcgcgccggc ggtggcagtt ctgctttgcg ccttcctcgt cgccttcttc ccaattctcg
     2221 ccaatacgac cctcgggctt gcctcggcgg ggcgaaatct gcgcgatctc tttgcgcttt
     2281 atggcgcctc gccctggcag accttgtggc gtctcaaaat accagccgcg ctgccgctct
     2341 tttttgccgg ccttcgtatc ggcggtggcc tcgctctgat cggcgccatc gcggcggagc
     2401 tgacggctgg tacgcagggg ggccaatccg gcctcgcttt tcgcatcgtc gaggcaggct
     2461 accggctgaa cattccgcgc atgtttgcgg cactcgcgct cgtcgcggtg actggcatcg
     2521 ccatctatgt cgcgctcgca gccttgtctc attgggtttt gtgccgttgg gatgagcgtg
     2581 cgtccgacgc ggcatgagct tgctaagcgc gacggcggat gctacccggc gctgacaatt
     2641 ccccggccgc tgaagtccat gacgatcaat ttccataaag gcgatctacc caacggcctg
     2701 agcttcggcg acagcgtcgc gatcgatacc gagacgctcg gccttgtgcc gtatcgcgac
     2761 cggctgtgcg tggtgcagct ttcggctggc gatggcgacg cccatgtcgt ccagatcgcg
     2821 gctggacaga cggcggcgcc gaacctggcg cggctcctcg ccgatccggc cgtgacgaag
     2881 ctatttcatt tcgcgcgttt cgatattgcg gtgctttatc gggccttcgg cataatggcg
     2941 gcgccgctct attgcaccaa gatcgcctcg aaactgaccc gcacctacac cgatcggcac
     3001 gggctgaaag atctcgtccg ggagcttttg ggcgtcgacc tctcgaaaca gcagcaatcc
     3061 tcggattggg cggcggaaag cttgagcgag gcccaactcg cctacgcggc ctccgacgtg
     3121 ctgcatctgc acgcgctccg cggcaagctc gacaccatgc tggcgcggga gaaccgtacc
     3181 gaactcgcgg cgtcctgttt cgagtttttg ccaacccgcg ccaagctcga tcttgccggt
     3241 tggccggatt cggacatttt ttcacacgaa taggcattct cgtggcagtc aagccgcgta
     3301 cggatagccc cgcgtaaacg ccgttcacgg gaattcccgt ggatttgaaa gggcatggcg
     3361 ccgcacccat gccataggat agaatagcct aagatttgtg aggaatactc cggccgccgc
     3421 ccgccttatc gggcggggtc gtgaattcgg caaaattgcc gcttagccgc tggacatgga
     3481 tttgcggatg acggatctca cgcgggcacc cgcgacgggt acaagccggc tcgatctttc
     3541 tgcgggcgcg tcacgccatg ccgtgcggca cgcccgttgg cactctattt tcgttcgcta
     3601 tctgcgccat ttcattatcg ccggtgccgc gctggttgtg ctgatcgtgg gtttcctgat
     3661 cctgttcaat ccattccggc agcttcagaa aaatctgacg agcggcgccg tggggatcaa
     3721 cgggacgatt gtcaccctgt ccacgccgaa aatgcaaggc gttcgccaaa atgggcagcc
     3781 cttcgaactg accggcgcct ccggcacgca ggacatcctg aacccgtccg tcatcaagct
     3841 tgtcggtgtt gatgccaagg ttgggctcga cgatcgcacc acggcacgaa tcaccgcgct
     3901 ctcgggtgtt tatgacagct cgcgtgacta tgtctggctc aaccagaatg tgcggatcaa
     3961 gaataccgac gcgggctacg acatgtttac tcaaaaggcc gaaatagatt tcgtctccgg
     4021 caa
//
LOCUS       DASWOG010000663         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_16951,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000663 DASWOG010000000
VERSION     DASWOG010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16951"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1536
                     /gene="hemA"
                     /locus_tag="VGB93_13575"
     CDS             232..1536
                     /gene="hemA"
                     /locus_tag="VGB93_13575"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY0147146.1"
                     /translation="MEYKAFFEDALGRLKAEKRYRVFVDLERDAAHFPYARWHRNADT
                     TEDVIMWCSNDYLGMGLDPQVIEAMCTAACRHGVGAGGTRNIAGTNHPLVALEAELAD
                     LHGKPGSLVFTSGWISNLAALSAIASLLPDCLILSDAYNHNSMIEGIKRSGAERQIFR
                     HNDLDHLEDLLKTAGRERPKLIAFESLYSMDGDIAQIGKIADLAERYNAMTYLDEVHA
                     VGLYGRRGGGIADRDNEMDRIDVIEGTLAKGFGTLGGYIAADLPIVDAVRSYAASFIF
                     TTALSPALAAAAQKAVQLLKSESGTELRARHQRQATLTKHALSAAGLPVMPNQSHIVP
                     VFVGDAVRCKAASDMLLERHSIYIQPINYPTVARGTERLRITPSPAHSDGHIAHLVEA
                     LVDVWQALKLPFTEMAANVVQFRRETDDAHCTFPELVKKAAE"
     gene            complement(1544..1789)
                     /locus_tag="VGB93_13580"
     CDS             complement(1544..1789)
                     /locus_tag="VGB93_13580"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0147147.1"
                     /translation="MRASSLLTLVQLVEAGMGITLLPKIAHEAVGGTRVQLIPYAAPK
                     AERTLVLVWRKGAVRADDYRLLAAHIRAHCLPVLTEA"
     gene            complement(1768..2454)
                     /locus_tag="VGB93_13585"
     CDS             complement(1768..2454)
                     /locus_tag="VGB93_13585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0147148.1"
                     /translation="MNVNVNLRHLRYLVTLSESSTFASAAKAAAITQSTLSAAIQGLE
                     AEFGHQLIDRSGRRMQLLPFGEAMVRRARHILAEIQELPEYASREILPLGTRLRLGVI
                     PSVAPFVLPRLLSSLSQSYPQLHLRVREGLTQSLLTELSSGRLDAAFIAHVPALDGIE
                     MIELAKDPFYLAVKAGHPLTRLKEVTMAQLAHEPLLLLDQGHCLREHAMEALGRDAIA
                     EETTCAPHRC"
     gene            2606..3160
                     /locus_tag="VGB93_13590"
     CDS             2606..3160
                     /locus_tag="VGB93_13590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0147149.1"
                     /translation="MAGIGDKLPSFSVTGVKPGFNNHEENGVSAFEPLTQESFPGKWK
                     IIFFYPKDFTFVCPTEIAEFAKLGKEFEDRDAVVLGGSTDNEFVKLAWRRSHKDLDRL
                     PIWSFADTNGSLTDGLGVRSPDGVAYRYTFIVDPDNVIQHVYATNLNVGRAPKDTLRV
                     LDALQTDELCPCNREVGGETLKAA"
     gene            3309..3827
                     /locus_tag="VGB93_13595"
     CDS             3309..3827
                     /locus_tag="VGB93_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004682000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0147150.1"
                     /translation="MSLDALKNQIADFAKDVRLNLSSMASDETLTPLQKYGLFAACGI
                     ASRNPQVRQALVAEAAQHLDETSLNAAKSAAAIMGMNNVYYRFLHLASNEEFRTMPAK
                     LRMNVIANPGVPKQDFELWSLGVSAINGCGMCIDAHEAELRKAGVSSQSIQAAVRFAA
                     IIQSAAIAVEAA"
     gene            complement(3895..5411)
                     /gene="pnp"
                     /locus_tag="VGB93_13600"
                     /pseudo
     CDS             complement(3895..5411)
                     /gene="pnp"
                     /locus_tag="VGB93_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614793.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
BASE COUNT         1019 a         1683 c         1642 g         1067 t
ORIGIN      
        1 ctagcaaggc ttcgccagcg catttcctta agatttgact gccttgatta ggatcaagaa
       61 tttcgcattt ttagctggga ctcttcgccc acttgcgccg tatttagaat taggcgaaaa
      121 ccaatctcgg ggaagcgacg agccagttca ggcagatccg cggtgcagac cgggagcgcc
      181 cagaaggcgc aaaagttggc tataggcgcc ttataaggac cgggttcgtc tatggaatat
      241 aaggcatttt tcgaagacgc tttgggccgt ctcaaagccg agaagcgtta ccgcgttttc
      301 gtcgatctcg aacgcgacgc cgcgcatttc ccttatgccc gctggcaccg caatgccgac
      361 accaccgaag acgttatcat gtggtgttcg aacgattacc ttggcatggg tctcgatccg
      421 caggtgatcg aggcgatgtg taccgccgcg tgccgccacg gcgtcggtgc gggcgggacg
      481 cgcaatattg ccggtaccaa tcatcctctg gtcgcgcttg aagccgaact ggcggatttg
      541 catggcaagc cgggcagcct cgtcttcacc tcgggctgga tttccaatct cgcggcgctt
      601 tccgcgatcg cttcgctgct gccggactgc ctgatcctgt ccgacgccta taatcacaat
      661 tccatgatcg agggcatcaa gcgctccggc gcggagcggc agattttccg ccacaacgac
      721 ctcgaccatc tcgaagatct gcttaagacg gccggccgcg aacggccgaa gctgatcgcc
      781 ttcgagagct tatattcgat ggatggcgat atcgcgcaga ttggcaagat cgccgatctc
      841 gctgagcgct acaatgcgat gacctatctc gacgaggttc acgccgtcgg tctctacggc
      901 cggcgcggcg gcggcattgc cgatcgtgac aacgagatgg accgcatcga cgtcatcgaa
      961 ggcacgctcg ccaaaggttt tggcactttg ggcggctata tcgcggcgga tctgccgatc
     1021 gtcgatgccg tgcgctccta cgcggcttca ttcatcttca cgaccgctct gtcgccggcg
     1081 ctggccgccg cggcgcaaaa ggcggtgcaa ttgttgaaaa gcgagtcggg gaccgaattg
     1141 cgtgcccgcc atcagcgtca ggcgacgctg acgaaacatg cgttgtcggc cgccggtctg
     1201 ccggtgatgc ccaatcaatc gcatatcgtg ccagtcttcg tcggcgatgc cgtgcgctgc
     1261 aaggccgcgt ccgacatgct gctcgaacgc cacagcatct atatccagcc gatcaattat
     1321 ccgaccgtcg cgcgcggcac ggagcgcctg cgcatcaccc cgtcgccggc gcattccgac
     1381 ggccatatcg cgcatctcgt cgaggctttg gtcgatgtct ggcaggcgct gaaactgccc
     1441 ttcaccgaga tggcggccaa tgtcgtgcaa ttccgccgcg agacggacga cgcacactgc
     1501 accttccccg agctcgtcaa gaaggcggcg gaatagccgc ctctcaggcc tccgtcaaaa
     1561 ccggcaggca atgcgcgcgg atatgcgccg cgagcagccg gtaatcgtcg gcgcgcaccg
     1621 cgcccttgcg ccagaccagc accagcgtcc gctctgcctt cggcgcggca taggggatga
     1681 gctggacccg cgtacccccg acggcttcgt gcgcgatctt cggcagcagc gtgatgccca
     1741 tgccggcctc gacgagttgc accagagtca gcagcgatga ggcgcgcacg tcgtttcttc
     1801 ggcgatggcg tcgcgcccca gcgcctccat cgcgtgctcg cgcaggcaat ggccttggtc
     1861 gagcaggagc aacggctcgt gcgcgagctg cgccatcgtc acctccttga gccgggtgag
     1921 cggatgaccg gctttgaccg cgagatagaa cgggtccttg gcaagttcga tcatctcgat
     1981 gccatcgagc gccgggacat gcgcaataaa ggcggcgtcg agccggccgc ttgagagttc
     2041 ggtaagcagg gactgcgtca gcccttcgcg gacgcgaaga tgaagctgcg gatagctctg
     2101 gctgaggctc gacagcagtc tcggcagcac gaacggcgcg accgagggga tgacacctag
     2161 ccgcagccgc gtaccaagtg gcaagatttc gcgcgaggca tattccggca attcctggat
     2221 ttcagcgagg atatgccggg cgcgacgcac catcgcctcg ccgaagggca ggagctgcat
     2281 ccgccgcccc gaccggtcga tgagctgatg cccgaactcg gcctcaagtc cttgaatcgc
     2341 ggcacttaac gtcgattggg tgatggccgc cgccttggct gccgaggcaa atgtgctgct
     2401 ctcggacagc gtcaccagat agcgcagatg gcgcaggttc acattgacgt tcatggcggg
     2461 cttatatcgg aaaaaacgaa cgcagataca aaaacattcg gcttgatcga aatttccaaa
     2521 cctcgcacag tgcgatgcag catcacccgt ttttgggtgc cggcggatgc ctcctcccac
     2581 tacgcgccgg caaaggaata atggaatggc tggaatcgga gataaactgc cctcgttctc
     2641 agtgactggc gtcaagccgg gcttcaacaa ccacgaggaa aacggcgtct cggcgttcga
     2701 gccgctgacg caagagagct tcccaggcaa gtggaagatc atcttcttct atccgaagga
     2761 tttcaccttc gtctgcccga ccgagatcgc ggaattcgcc aagctcggca aggaattcga
     2821 ggatcgcgac gccgtcgtcc ttggcggctc gactgacaat gaattcgtca agctcgcctg
     2881 gcgccgcagc cacaaggatc tcgacagact gccgatctgg tccttcgccg ataccaatgg
     2941 ttcgctgacc gatggccttg gcgttcgttc gcccgacggc gtcgcctatc gctacacgtt
     3001 cattgtcgat ccggacaatg tgatccagca cgtctatgcg acgaacctca atgtcggccg
     3061 tgcgccgaag gacacgctac gcgtgctcga cgcgttgcag acggacgagc tttgcccgtg
     3121 caatcgggaa gtcggcggcg agacgctgaa ggccgcgtaa gccttctgca aatttcgcga
     3181 cgatacggcg gggccaaaag ctccgccgtt tcgtttcgcg cctctctctt gtgttttgac
     3241 gcaattccgg acgcaaaacc gcaccgcatt tttgctggaa ttgctttgat gaaggatcag
     3301 aatccgaaat gtcgctcgac gcattgaaaa accaaatcgc cgatttcgcc aaggacgtgc
     3361 ggctcaatct ctcgtcgatg gcgagcgacg aaacgctgac gccgttgcag aaatacgggc
     3421 tcttcgccgc ctgcggcatc gcctcgcgca atccgcaggt gcgccaggcg ctcgtcgccg
     3481 aggctgcgca gcatctcgac gagacgtcgc tcaatgccgc caagtcggcc gcggcgatca
     3541 tgggcatgaa caacgtctat taccgtttcc tgcacctcgc ctcgaacgag gaattccgta
     3601 ctatgccggc gaagctgcgc atgaacgtca tcgccaatcc gggcgtgccg aagcaggatt
     3661 ttgaactctg gtcgttggga gtgtcagcca tcaacggctg cggcatgtgc atcgatgcgc
     3721 atgaggccga attgcgcaag gccggcgtca gttcgcagag catccaggcg gcagtgcgtt
     3781 ttgctgcgat catccaatcg gcggcgatcg cggtcgaagc ggcctgaaaa tgaatcatgc
     3841 gcggattgct ccgcgcatga tctttatctg taagaagcta gtcgcgcgga aagcttaatc
     3901 tgcggcgccg gcgtcttcag ccttttcctt ggcttcgaga tcttcgcccg tctgctgatc
     3961 gacagcacgc atcgagagcc gcaccttgcc gcggtcgtcg aagcccagca gctttacttt
     4021 taccttgtcg ccttccttga cgacatcggt cgtcttggcg acgcggccct tggcaagctg
     4081 cgagatgtgg acgaggccgt ccttcgagcc gaagaagttc acgaaggcgc cgaactcgac
     4141 cgccttcacg acggtgccgt cgtagatatg gccgatctcc gggtccatcg cgatcgactt
     4201 gatccaagcg accgcggcct tgatcgaatt ggcgtcgctc gaggcgacct tcaccgtgcc
     4261 gtcgtcctca atgttgatct tggcgccagt cttttcgacg atctcgcgga tcaccttgcc
     4321 gccggtgccg atgacttcgc ggatcttgtc ggttgcgatc ttgaagctct cgatacgcgg
     4381 tgcgaattcg ccgagttcgg cgcgggcgcc agtcagcgcc ttggccatct cgccgaggat
     4441 gtgcagccgg ccgtcctttg cctggccgag cgcaacacgc atgatctctt cggtaatacc
     4501 ggcgatcttg atgtccatct gcagcgaggt gataccggat tcggtccccg ccaccttgaa
     4561 gtccatgtcg ccgagatggt cttcatcgcc gagaatgtcg gagagcacgg caaagcgctc
     4621 gccttcgagg atgaggccca tggcgatgcc agccgtcggc gccttgagcg gtacaccggc
     4681 gtccattagc gcgagcgacg agccgcagac cgtcgccatc gacgacgagc cgttcgactc
     4741 ggtgatctcg gagacgacgc ggatcgtgta ggggaattcc gcggccgtcg gcagcatcgg
     4801 acggatcgca cgccaggcga gcttgccgtg gccgatttcg cgccgaccag gcgaacccat
     4861 gcggcccgtc tcgccaaccg aatagggcgg gaagttgtag tggagcagga agcgctcctt
     4921 gtaggtgcct tcgagcgagt cgatgtattg ctcatcctcg ccggtgccga gggtcgcgac
     4981 gacaagcgcc tgcgtctcgc cgcgggtgaa aagcgccgaa ccatgcgtgc gcggcagaat
     5041 gccgacctgc gccaggatcg gacgcacggt cttcacgtcg cgcccgtcga tacggatgcc
     5101 gttgtcgaga atgttccagc gcacgacctt cgcctggaga tcgtggaagg cttcgccgac
     5161 ctgctccttg gtgaacttgg cgccctcggg cggcaacagc gccgccgtca ccttcgcctt
     5221 caccgcgtcg acggcagcat agcgcacggc cttggcggtg atcttgtagg cttcgcgcag
     5281 ttccgcttag gcgacgccgc gaacggcgtt ctcgagctcg gttttgtcga cagtggtgag
     5341 atcgcgcggc tccttcgccg ccttttcggc gaggcggctg atcgcctcaa tcacgggttg
     5401 gaagccccaa t
//
LOCUS       DASWOG010000664         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_145336,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000664 DASWOG010000000
VERSION     DASWOG010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145336"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VGB93_13605"
     CDS             <1..1259
                     /locus_tag="VGB93_13605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY0147151.1"
                     /translation="SLRFKIGGEELALEPTLNFMQDTKEDVRKEAAAAIADTLSQNLR
                     TFAFITNVLAKDKEIADRWRGFKDVADSRHLANRVEREVVDALAAAVQSAYPRLSHRY
                     YKLKARWFGQDALDFWDRYAPLPNMPQRTYAWVDARNTVLGAYAAFSPRMAGIAEEFF
                     KRNWIDAPVRPGKAPGAFAHPTVPSAHPYVLLNYQGKPRDVMTLAHELGHGVHQVLAA
                     PNGALMANTPLTLAETASVFGEMLTFQALLAKAQSVAERRAMLAAKVEDMINTVVRQI
                     AFYAFERKVHVERRNGELTREKICELWMSVQSESLGPAIRLGPGYETYWAYIPHFIHS
                     PFYVYAYAFGDCLVNSLYGVYQRVSEGFAERYLAMLAAGGSKHYSELLAPFGLDAKDP
                     AFWQIGLGMIESLIDELETMDWQITI"
     gene            1408..>1974
                     /locus_tag="VGB93_13610"
     CDS             1408..>1974
                     /locus_tag="VGB93_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0147152.1"
                     /translation="MHFTSYLGIAWLAIAWFVFLATCAGALLQPLFQARRARNNAQPP
                     VTAVLPIKLEHSGFDKAQASVFGQDYPEYEVLFSAAEADSPALETAFALKAHYPQTPT
                     RILRSQSDFAVSPKLNTLDAPLATSAHDFVLVKDSTIVLEHDMLATFMRNFGAGVGMV
                     VGVPVAEQGQTVAGHIESAVINAHARLLL"
BASE COUNT          368 a          585 c          589 g          432 t
ORIGIN      
        1 ccagcctgcg cttcaagatc ggcggcgaag aactggcgct ggagccgacg ctcaatttca
       61 tgcaggatac gaaggaagac gtgcgcaagg aggctgccgc cgccattgcc gacacgctct
      121 cgcagaacct gcgcactttc gccttcatca ccaatgtcct cgccaaggat aaagagattg
      181 cggaccgctg gcgcggtttc aaggatgtgg ccgattcacg ccatctcgcc aatcgcgtcg
      241 agcgcgaggt cgtcgatgcg ctcgccgcgg cggtgcaatc ggcctatccg cgcctctcgc
      301 accgctatta caaattaaaa gcgcgctggt tcggccagga cgcgctcgat ttctgggatc
      361 gctatgcgcc gctgcctaat atgccgcagc ggacttatgc ctgggtcgat gcacgcaaca
      421 cggtgctcgg cgcctacgcc gccttctcgc cgcgcatggc gggcattgcg gaagaatttt
      481 tcaaacgcaa ttggatcgac gcgccggtgc ggccgggcaa ggcgccgggc gctttcgcgc
      541 atccgacagt gccgtccgcg catccctatg tcttgctcaa ttatcagggc aagccgcgcg
      601 acgtcatgac gctggcgcac gagcttggcc acggcgtgca tcaggtgctc gccgcgccaa
      661 acggcgcgct gatggcgaat acgccgctga ctctggctga gacggcttcc gtctttggtg
      721 aaatgttgac gttccaggcg cttctcgcga aggctcaatc cgtcgctgaa cgccgggcca
      781 tgctcgcggc caaggtcgag gacatgatca acacggtcgt gcggcagatc gcgttctacg
      841 cgttcgagcg caaggtgcat gtggagcggc gcaatggcga actgacgcgc gaaaaaatct
      901 gcgaactctg gatgagcgtg caaagcgaga gccttggccc ggcgattcgc cttggccctg
      961 gctatgaaac ctattgggcc tatatcccgc acttcattca ttcgccgttt tacgtctacg
     1021 cttacgcttt cggcgattgt ctggtgaatt cgctctacgg cgtttaccag cgagtaagcg
     1081 agggctttgc cgaacgatat ctcgccatgc tcgccgccgg tgggagcaag cattattctg
     1141 agcttcttgc gccgttcggg cttgatgcca aggatccggc cttctggcag atcgggctcg
     1201 gcatgatcga aagccttatc gacgaacttg agacgatgga ttggcagata actatctgat
     1261 tcgtaagcga tcttgagctt gacgtcgaaa tgtggtcgag tggcgccgca ttgacgtttc
     1321 agccccgccg tgggattgcg tatgtgctgg gggaatcagg ccggtacgat cgcggcgagc
     1381 tgagtgcggg gggcaagctc aataagcatg catttcacat cctaccttgg tattgcatgg
     1441 cttgcgattg cgtggttcgt cttcctcgca acatgcgctg gcgctttgct gcagccgttg
     1501 tttcaagcgc ggcgggcgcg caacaatgcg cagccgccag tcaccgcagt tctgccgata
     1561 aaattggaac attcgggctt cgacaaagct caagcctcgg tcttcggtca ggattatccc
     1621 gaatacgaag ttctgttcag cgccgccgag gcggattcgc cagctttaga gaccgctttc
     1681 gcgctgaagg ctcactatcc gcaaacgccg acgcgcatcc ttcgttcgca gtccgatttc
     1741 gcggtgagcc ccaagctcaa tactcttgat gctccgctcg ccacgtcagc gcacgatttt
     1801 gttctcgtca aggattccac catcgtattg gagcacgata tgctcgcgac tttcatgcga
     1861 aatttcggcg cgggcgtggg catggttgtt ggtgttccgg tcgccgaaca ggggcaaact
     1921 gttgccggcc acatcgaatc tgccgtcatc aatgcgcatg cgcgcttgct gctc
//
LOCUS       DASWOG010000665         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_291048,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000665 DASWOG010000000
VERSION     DASWOG010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291048"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VGB93_13615"
     CDS             complement(<1..167)
                     /locus_tag="VGB93_13615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEY0147153.1"
                     /translation="MQVAIGHYLVVAAILFTLGVAGIIVNRKNIIVILMSVELILLAV
                     NINLVAFSAFLG"
     gene            complement(170..778)
                     /locus_tag="VGB93_13620"
     CDS             complement(170..778)
                     /locus_tag="VGB93_13620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0147154.1"
                     /translation="MNTVTFFFYLFSAIMIASAFMVITARNPVHSVLFLILAFVNAAG
                     LFLLLGAEFLAMILIIVYVGAVAVLFLFVVMMLDVDFAEFKEGFQRNLPFGVTVGGVV
                     LVELAFCVAAWHIGSSTLGQTASPTPQGLTNTAALGRVLYTQYAYLFEASGLVLLTAM
                     IGAIVLTLHHKPGVKRQNIAEQNARTAATAVEIKKQPSRAGV"
     gene            complement(846..1334)
                     /gene="nuoI"
                     /locus_tag="VGB93_13625"
     CDS             complement(846..1334)
                     /gene="nuoI"
                     /locus_tag="VGB93_13625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY0147155.1"
                     /translation="MKIEAAAKSLLLKEFVGAFFLSMRYFFKPKATLNYPHEKNPQSP
                     RYRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVKC
                     IYCGFCQEACPVDAIVEGPNAEFSVETREELYFDKGRLLDNGDRWESEIARNIEIDAP
                     YR"
     gene            complement(1344..>2258)
                     /gene="nuoH"
                     /locus_tag="VGB93_13630"
     CDS             complement(1344..>2258)
                     /gene="nuoH"
                     /locus_tag="VGB93_13630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0147156.1"
                     /translation="WAAVQFRRGPNVVGPWGLWQSFADLIKFVLKEPIIPDSANKGLF
                     LLAPVLTGFLALAAWAVIPIGKGWSIANINVGILYIFAISSLSVYGIIMGGWASNSKY
                     PFLSALRSAAPMVSYEVSIGFVLVTVLLCVGSLNLEDIVKAQDSRFGLLGWYWLPLLP
                     MFVIFFVSALAETNRPPFDLVEAESELVAGFMVEYSSAPYLLFMLAEYVAIITMCVLG
                     TILFLGGWLSPIPFAPFTWIPGPIWFVLKVCLLFFLIAMVKAFVPRYRYDQLMRLGWK
                     VFLPISLVMVVLVAAVLEFTGWGGSMAQ"
BASE COUNT          562 a          673 c          643 g          380 t
ORIGIN      
        1 ccgaggaaag cggaaaaggc gacgaggttg atattgaccg ccagcaggat caactcgacc
       61 gacatcagga tgacgatgat gttcttgcga ttgacgatga tgccggccac gccgagcgta
      121 aacaggatcg cggcgacgac aagataatgc ccaatcgcaa cttgcatcgt cagacccccg
      181 cccgtgatgg ctgtttcttt atttccaccg ctgtcgctgc ggtgcgcgcg ttctgctcgg
      241 cgatattctg gcgcttcaca cccggcttat gatgcagcgt caatacgatt gcaccgatca
      301 tcgcggtcag gaggacgagc cccgaggcct cgaagagata ggcatattgc gtgtaaagca
      361 cgcggccaag cgccgccgta ttggtgaggc cttgcggcgt cggcgaggcc gtctgaccca
      421 gcgtgctgct accgatgtgc caggcggcga cgcaaaaggc gagctctacc agaaccacgc
      481 cgccgaccgt cacgccaaac ggcagattgc gctgaaagcc ttctttaaat tcggcgaaat
      541 cgacatcgag catcatgacg acgaagagaa acagaaccgc gaccgcaccg acatagacga
      601 tgatcaggat catcgccaaa aattccgcgc cgagcagcag gaaaagcccc gccgcattga
      661 cgaaagcgag aatgagaaac aataccgagt gaacgggatt gcgcgccgtg atcaccatga
      721 aagccgaggc aatcatgatc gcggaaaaaa gatagaagaa gaaggtgacg gtgttcatcg
      781 gtcgcgccct cccctcggct tatctgtcgc caccgcgttt cctcccgcgc acctttgcat
      841 cattatcacc gatagggcgc gtcgatctca atgttgcggg cgatttcact ctcccaacgg
      901 tcgccgttgt ccagcaggcg ccccttgtcg aaatagagtt cttcgcgagt ctcgaccgag
      961 aattcggcat tcggcccctc gacgatcgcg tcgaccgggc aggcctcctg gcagaagccg
     1021 caataaatgc atttcaccat gtcgatatcg tagcgcgtcg tgcggcgcgt gccgtcgtta
     1081 cggcgcggcc ccgcctcgat cgtaatggcc tgcgccgggc agatcgcctc gcaaagcttg
     1141 caggcgatgc agcgctcttc gccattcgga taacggcgca gcgcgtgttc gccgcgatag
     1201 cgtggcgatt gcggattctt ctcgtgcgga tagttcagcg tcgctttcgg cttgaagaaa
     1261 taacgcatcg acagaaagaa ggcgccgacg aattctttca gcaaaagcga tttggcagcc
     1321 gcttcgattt tcatcgcatc acctcattgc gccatcgagc cgccccagcc ggtgaactca
     1381 agcaccgcgg cgacaaggac gaccatgacc agagagatcg gcaaaaacac cttccagccc
     1441 agccgcatga gctgatcgta gcgataacgc ggcacaaagg ccttcaccat cgcgatcagg
     1501 aagaacagca aacagacttt caggacaaac cagatcggac ccggaatcca ggtgaaaggt
     1561 gcaaacggaa tcggcgacag ccagccgcca aggaacagga tcgtccccag cacgcacatg
     1621 gtgatgatcg ccacatattc ggcgagcata aacagcaaat agggcgccga ggaatattcg
     1681 accataaaac cggcgacgag ctcggattcc gcctcgacga gatcgaaagg cggccggttc
     1741 gtctccgcca aggccgaaac gaagaagatc acaaacatcg gcaggagcgg cagccaatac
     1801 cagccgagaa ggccaaagcg cgaatcctgc gccttgacga tatcttcgag gttcagcgag
     1861 ccaacgcaga gcagaacggt gacgagcacg aagccgattg agacttcgta agagaccatc
     1921 ggcgcggccg agcgcagtgc cgacaggaac ggatatttcg agttcgacgc ccagccgccc
     1981 atgatgatgc catagaccga gagcgatgag atcgcgaaaa tgtagagaat gccgacattg
     2041 atattggcga tgctccagcc ttttccgatc ggaataaccg cccaggcggc gagcgcaaga
     2101 aaacccgtga ggaccggcgc cagcaggaac agacctttgt tcgcgctgtc cgggatgatg
     2161 ggttccttca gcacgaattt gatcaaatcg gcgaagctct gccacaggcc ccagggaccg
     2221 acgacattcg gcccgcgccg aaattgcacc gccgccca
//
LOCUS       DASWOG010000666         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_273867,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000666 DASWOG010000000
VERSION     DASWOG010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273867"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGB93_13635"
     CDS             <1..243
                     /locus_tag="VGB93_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0147157.1"
                     /translation="VEAVQRKGVRVSVVSTNTTQPAMVADELRRQADEFIDLIHLSSK
                     IGRDPNERPERAQKFHPERRQGATVSAGYDEDMLED"
     gene            351..1046
                     /locus_tag="VGB93_13640"
     CDS             351..1046
                     /locus_tag="VGB93_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0147158.1"
                     /translation="MTVLEPDLQTTVGPDAPSHDCPLCPRLVAFRTDNQAEHPDWFNA
                     PVPTFGTRDASLLIVGLAPGLRGANRTGRPFTGVYAGDLLYATLLDYGFATGRYAQRP
                     DDGLTLIDCAITNAVRCVPPQNKPLPEEIANCRRFLTATIATMPNLRAIVALGRIAHD
                     SLVRAAGEKLSAWPFAHGRAHRLAKLGARNVELFDSYHCSRYNTNTGVLTVEMFQALF
                     ARVQEFLRTSHSS"
     gene            complement(1024..1410)
                     /locus_tag="VGB93_13645"
     CDS             complement(1024..1410)
                     /locus_tag="VGB93_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0147159.1"
                     /translation="MLNLKIDHCVIHVSDWERSNHFYAAVMGADVVPRQKGFAYKFGD
                     FLLNCHGPGVVAKAVARVPVAPGGSDLCFEWPGPLEEAIAHLEEQHIRIELGPVATRG
                     AKGEATSVYFRDPDGSLLEFMSYVKS"
     gene            complement(1423..1899)
                     /gene="smpB"
                     /locus_tag="VGB93_13650"
     CDS             complement(1423..1899)
                     /gene="smpB"
                     /locus_tag="VGB93_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0147160.1"
                     /translation="MAEKKETSFKVVADNRRARFDYEIGETFEAGLVLTGTEVKSLRT
                     GKASIGESYASLDKVGELYLLNANIPEYLQANRFNHQPKRARKLLLNTREISRLAKGL
                     EREGMTIVPLKIYFNDRGRAKLELALGRGKKLHDKRETEKKRDWNREKSRLLRDRG"
     gene            complement(1912..2805)
                     /gene="dapA"
                     /locus_tag="VGB93_13655"
     CDS             complement(1912..2805)
                     /gene="dapA"
                     /locus_tag="VGB93_13655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0147161.1"
                     /translation="MGKKASFKGSITALVTPFAAGQFDEGAYRALVDWQIVNGTHGLS
                     PVGTTGESPTLTHEEHKFVVEVCVAEARGRVPIIAGSGSNNTQEAIDLARHAEKVGAD
                     GVLVVAPYYNKPSQEGLYQHYKAINDAIGIPIIVYNIPPRSVVDISIDTMKRLFELKN
                     IAGVKDATANVARIALQRRALGADFIQLSGEDMTALAVLAHGGDGCISVTSNVAPRLC
                     ADFQDAGLKGDFKAALAIQDRLTPLHAALFVDPNPAGPKYALSLLGRIADELRLPMLS
                     ATPPAQVAVRVAMEHAGLLPK"
     gene            3110..5323
                     /locus_tag="VGB93_13660"
     CDS             3110..5323
                     /locus_tag="VGB93_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0147162.1"
                     /translation="MAVRRQTLRVPWLLCSLGLTFVGLITVISILPAPDFNGTAPART
                     QMLSWLGDLFNDVAGNLEHFLASGHVHLQLANDEHPAITPPIDPVVALGLNPTGLREA
                     LPYYKAGELDAGDAQAVRATDPLIRTALEWVALRDSRNAGRDRLGTFLDEHPNWPARE
                     YLMRRIEDGLYQSNADPALIANFFGAAAPQTALGKLAKARALRSEGKDTDAEALVRDV
                     WRHSTLPASLETHVRAEFGAYLSPADHKARADRLLYEGDNAGGLRNAALVNPDYVVLA
                     RLHAAINDEAANDKMFDAVPPDMRADPAFLFARIHKLRHEDKLRDAATLMLAAPRDPA
                     LISDGDAWWAERRLLGRKLLDAGDPATAYRIFAGHSAESPEANIDAEFHAGWIALRFL
                     NDPARAAQHFARAATFAQTPMSIARTAYWQGRTAEVSLTDDADLRARTYYEKAAERAA
                     TYYGQLARARLGLKTIALNAPVRAARGPARDEAVRIVELFFTIHEKDLAMALANSTAQ
                     HLTDPAQIAALADVMLAQRDAHDCLIIGKTLAQRGTLIDALAFPTYGIPAYTAAENSA
                     PPALVYAIARQASEFDMHAQSGAGARGLMQMIVATAKRTAEHLKMDFDAGRLLSDAAF
                     SAKLGAAHLGQLLAEERGSPILVFAAYNAGGGHVKDWIDAYGDPRTPGVDPIDWVERI
                     PFTETRNYVERVSENWAMYRASFAALDAAQASTGVRSAQVTPAKVGTTLEASAGL"
BASE COUNT          996 a         1689 c         1612 g         1059 t
ORIGIN      
        1 gtcgaggcgg tgcaacggaa gggcgtacgg gtttccgtcg tttcgaccaa tacgacccag
       61 ccggccatgg tggcggacga attgcgccgc caggccgacg agttcattga cctcatccat
      121 ctttcctcga aaatcggacg cgatccgaac gagcggccgg agcgcgcaca gaaatttcat
      181 ccggaacgtc ggcagggcgc taccgtctcc gccggctatg acgaagacat gctcgaggac
      241 tgagcacgac gcggcaacgc cggtgaaccc gcgctgccgc gtcccgattg cgcggaattt
      301 ttcgggggtc ctgagttccg cttttgaatc gaatgtttta ggacgggtga gtgaccgtcc
      361 tggaaccaga tttgcaaacg accgttgggc cagatgcgcc cagtcacgac tgtccgctct
      421 gtccccgtct ggttgccttc cgaacagaca atcaggcgga acatcccgac tggttcaatg
      481 ccccggttcc gacgttcggc acgcgcgacg cgagtctcct gattgtcggc ctcgcaccgg
      541 gcttgcgcgg cgccaaccgg accggtcggc ccttcaccgg agtttatgcc ggcgatttac
      601 tctacgcgac tctgctcgat tacgggttcg cgacggggcg ctatgcgcag cggccggacg
      661 acggcctcac cctcatcgac tgcgcgatta ccaatgcggt gcgctgcgtg ccgccgcaga
      721 ataagccgct gccggaagag atcgccaatt gccgccgctt tctgacggcg accatcgcca
      781 ccatgcctaa tcttcgcgcc attgtcgcgc tcgggcgcat cgcgcatgat tctttggtgc
      841 gggctgcggg cgaaaagctt tcggcctggc cgttcgcgca tggccgcgca catcggctgg
      901 ccaaactcgg cgcccgcaac gtcgagttgt tcgatagtta tcattgctcg cgctacaaca
      961 cgaatacggg cgtgctgacc gtcgaaatgt ttcaggctct attcgcgcgc gtgcaggaat
     1021 tcctcaggac ttcacatagc tcatgaattc gagcagcgag ccgtccgggt cgcggaaata
     1081 gacgcttgtc gcctcgccct tggctccgcg cgtcgcgacc gggccgagtt cgatccggat
     1141 gtgctgttct tcgagatgtg cgatggcttc ttcgagcggt cccggccact cgaagcaaag
     1201 atcgctgccg cccggcgcga cgggaacgcg cgcgaccgct ttggcaacga caccgggccc
     1261 atggcaattc agcagaaagt cgccgaattt gtaggcgaag cccttctgcc gcggcacgac
     1321 gtccgcgccc atgacggcgg cataaaaatg attggagcgt tcccagtcgg aaacgtggat
     1381 cacgcaatgg tcgattttga gatttagcat tccactctcg ccttagccgc ggtcgcgcag
     1441 aagccggctt ttctcgcggt tccaatcgcg ctttttctcc gtctcgcgct tatcgtgcag
     1501 cttcttgccg cgtccgagcg caagctcgag cttggcgcgg ccgcgatcgt tgaagtagat
     1561 tttcaacggc acgatcgtca tgccttcgcg ttcaagtcct ttggcaagac gcgaaatttc
     1621 gcgtgtattc agaaggagtt tgcgggcgcg cttcggctga tgattgaagc ggttcgcctg
     1681 cagatattcc gggatgtttg cattgaggag atagagttcg cccactttgt cgaggctcgc
     1741 gtagctttcg ccgatcgaag ccttgccggt acgcagggat ttgacctcgg tgccggtcaa
     1801 gaccaatccg gcctcgaacg tctcgccgat ttcatagtcg aagcgggcgc ggcgattgtc
     1861 cgcgacgacc ttgaagctcg tctctttctt ttcagccaac gcacttgcct gttatttcgg
     1921 taaaagcccg gcgtgctcca tcgcgacacg gacagcaacc tgagcgggcg gcgtggcgga
     1981 caacatcggc aggcgcaatt cgtcggcaat cctaccgagc agcgacagag cgtatttcgg
     2041 ccctgctggg ttcggatcga caaagagtgc cgcgtgcagc ggggtcagcc ggtcctgaat
     2101 cgccaaggcg gccttgaagt cacccttgag gccggcgtcc tgaaaatcgg cgcagagccg
     2161 gggcgcgaca ttcgaggtca cggaaatgca gccgtcaccg ccatgcgcca gaacggcaag
     2221 tgcggtcata tcctcacccg agagctggat gaaatcggca ccaagcgccc ggcgctgcaa
     2281 ggcgatgcgc gccacatttg cggtcgcatc cttgacgccg gcgatgttct tcagctcgaa
     2341 gagccgcttc atcgtgtcga tcgaaatatc gacaaccgag cgcggcggaa tattatagac
     2401 gatgatcgga atgccaatcg cgtcgttgat ggctttgtag tgctgataaa gcccctcttg
     2461 cgacggctta ttgtaataag gcgcgacgac aagcacacca tcggcgccca ctttctcagc
     2521 gtggcgcgcg agatcgattg cctcttgcgt attgtttgac cccgacccgg caatgatcgg
     2581 cacacggccg cgtgcctcgg cgacgcagac ctcgaccacg aatttatgtt cctcatgggt
     2641 gagcgtgggg ctttcgcccg tcgtaccgac tggcgacaga ccgtgcgtgc cattgacgat
     2701 ctgccagtcg acaagagcgc ggtaggcgcc ttcgtcgaat tgtcccgcgg cgaatggcgt
     2761 caccaaagcg gtgatcgagc ccttaaaaga ggctttcttg cccattttcg gccgttcctt
     2821 ctttcttctt tggaatgcct tagttttgca cctgtctctt ccgacaattg tgcggtcaag
     2881 tccatgtgcg tgttgccgct gctttatgca aggcctgcca tgccggcccg taaagtgcgg
     2941 caagtctttg atttacaatc atatcgagcg acccgtcgcg gcttgtgccg cgtcgccagg
     3001 gcggatggaa ccgatgttgt taacgaatcg gcaagcagaa tgacaagatt cttgcgatct
     3061 cgcatgtcag cgggcgcttc gcaccaatcc aaggcggcgg gagcctgata tggccgttcg
     3121 gcgacagaca cttcgcgttc cctggctgct ttgtagcctt ggactcacgt ttgtcggctt
     3181 gattacggtg atctcaattc tgccggcgcc cgatttcaat ggcaccgccc ccgcacgcac
     3241 gcaaatgctc tcctggctcg gcgacctctt caacgatgtg gcgggcaatc tggagcattt
     3301 cctcgcctct ggtcacgtac atctgcagct ggcgaatgat gaacatccag cgatcacgcc
     3361 gcctatcgat ccggtggtgg cgctcggcct caacccgacg gggctgcgcg aggcgcttcc
     3421 ttattataaa gccggcgaac tcgacgcagg cgatgctcag gccgtccgcg cgactgaccc
     3481 actcattcga accgcgctcg aatgggttgc cctgcgtgac tcgcgcaacg ccggccgcga
     3541 ccgtctcggc accttcctgg acgagcatcc gaattggcca gcgcgcgaat atctgatgcg
     3601 tcggatcgag gatgggctct accaaagcaa cgccgacccc gccttgatcg ctaatttctt
     3661 tggcgccgct gcgccgcaga ccgcacttgg caagctggcc aaggcccgcg cgctcagaag
     3721 cgagggtaag gatactgacg cggaagcact ggtgcgcgac gtctggcgcc attcgacgct
     3781 cccagcctcg ctcgaaaccc acgtgcgcgc tgaattcggc gcctatctct cgccggcaga
     3841 tcacaaagcc cgcgccgacc ggctgctcta tgaaggcgac aatgcaggcg ggctgcgcaa
     3901 tgcagcgctg gtaaacccgg actatgtcgt gctcgccaga ttgcatgccg caatcaacga
     3961 cgaagccgcc aacgacaaaa tgttcgatgc cgtgccgccg gatatgcgcg ccgatccggc
     4021 cttcctcttt gcccgcatcc ataagctgcg ccacgaggac aagcttcgcg acgccgccac
     4081 tttgatgctg gccgcgccac gagacccagc actcatctcc gacggcgacg cctggtgggc
     4141 tgagcgccgg ctcctgggac gcaaattgct cgatgccggc gatccggcga cggcctatcg
     4201 gattttcgcc ggacattccg ccgaatcgcc agaggcgaat atcgatgcgg aattccacgc
     4261 tgggtggatc gcgctgcgct tcctcaacga tccggcgcgc gcggcgcaac atttcgcgcg
     4321 cgccgcgacc ttcgcgcaaa cgccgatgtc gattgcccgc accgcctatt ggcagggccg
     4381 caccgcggaa gtctcactca ccgacgatgc cgacctgcgc gcccgtacct attacgaaaa
     4441 ggcggccgaa cgtgcggcga cctattatgg tcaattggcg cgcgcgcggc tgggcctcaa
     4501 gacgattgcg ctcaacgcgc cggtgcgagc agcgcgcgga cccgcgcgcg acgaggcggt
     4561 gcggatcgtt gaactcttct ttacaatcca cgaaaaagat cttgcgatgg cgctcgccaa
     4621 tagcaccgct cagcatttga ccgatccggc gcagatcgcc gcgctcgccg atgtcatgct
     4681 ggcgcagcgc gatgcgcatg attgcctcat catcggcaag acgcttgcac agcgcggtac
     4741 tctgatcgac gccctcgcct tcccgaccta tggcattccc gcctacacgg cggcggaaaa
     4801 ctctgcgccg ccggcgctag tgtatgcgat cgcccggcag gcaagcgaat tcgatatgca
     4861 cgcgcagtcc ggcgcgggcg cgcgcggatt gatgcagatg atcgtcgcga ccgcgaagcg
     4921 cacggcggaa catttaaaaa tggacttcga cgccggccgg ctgctcagcg atgccgcctt
     4981 cagtgcaaaa ctcggcgcgg cgcatctcgg gcaattgctg gctgaagagc gcggttcacc
     5041 gatcctggtc tttgcggcct ataatgctgg cggcggccac gtgaaggact ggattgatgc
     5101 ttacggcgat ccgcgcacgc ccggcgtgga tccgatcgat tgggtggaga gaatcccgtt
     5161 caccgagacg cgcaattacg tcgagcgcgt gagcgagaat tgggcgatgt atcgcgcgag
     5221 cttcgcagcg ctcgacgccg cccaagcctc aaccggcgtg cgaagtgccc aggttacgcc
     5281 ggccaaggtc ggcacgacgc ttgaggcgtc ggcaggtcta taaggcctag tgaactgaca
     5341 ccgaagcagc gagatc
//
LOCUS       DASWOG010000667         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_181587,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000667 DASWOG010000000
VERSION     DASWOG010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181587"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VGB93_13665"
     CDS             <1..1431
                     /locus_tag="VGB93_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0147163.1"
                     /translation="IVLRHGSAGIYKRLVLKDNRIIGTVLFGETGDGAWFNDLKKKSV
                     DISDMRETLIFGQSYQGGAPLDPMAAVAALPDDAEICGCNGVCKSAITSAIAAKGLTS
                     LDDVRAHTKASASCGSCTGLVEQLLKLSLGEAYNPAAVQPMCNCTDLGHDDVRRLIKA
                     KSLKTIPAVMQALEWKTSCGCAKCRPALNYYLVCEWPDGYADDYQSRFINERAHANIQ
                     KDGTYSVVPRMWGGVTSAAELRAIADVVEKFAIPTVKVTGGQRIDMLGVKKEDLPAVW
                     ADLGQAGFVSGHAYAKGVRTVKTCVGTDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVK
                     MAVSGCPRNCAESTCKDVGVICVDSGYEIHFAGAAGLDIKSTEVLGLVQTEDEALEVI
                     VALVQMYREQARYLERIYKWTKRVGVAEIRRQILDDADKRQAYFDRFVFSQKFAQVDP
                     WEERVSRKHKHEFKPMAVIDFAPAAE"
     gene            1431..1766
                     /gene="nirD"
                     /locus_tag="VGB93_13670"
     CDS             1431..1766
                     /gene="nirD"
                     /locus_tag="VGB93_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEY0147164.1"
                     /translation="MIMNWIDIGTVTDIPRRGARCVVTPQGRIAVFRTMDDQFFAMED
                     RCPHKGGPLSQGIVHGNAVTCPLHNWVISLETGMALGADKGSVKTIPLRRDGEKLYLA
                     LDAAMRLAG"
     gene            1766..>4418
                     /locus_tag="VGB93_13675"
     CDS             1766..>4418
                     /locus_tag="VGB93_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0147165.1"
                     /translation="MEAAATKTTCPYCGVGCGVITSVAGDGSVAIRGDTEHPANYGRL
                     CSKGSALGETVDLDDRLLYPSIHGHRASWDEALDLVAGAFSSTIAEHGPDSVAFYISG
                     QLLTEDYYVANKLMKGFIGAANIDTNSRLCMASSVAGHRRAFGADTVPGTYEDLELAD
                     LIVLVGSNLAWCHPVLYQRIAAAMQKRPQMKIVLVDPRRTMTADIAEMHLAVRPDGDV
                     ALFNGLLAWLADHDALDRAYIAGHTSGFDDAFAAASAMSFDEIAEQTGVAADELRAFY
                     ALFARTEKVVTVYSQGVNQSSAGTDKVNAIINCHFATGRIGRAGMGPFSVTGQPNAMG
                     GREVGGLANMLAAHMEIENAEHRDCLQRFWRAPTMAERPGLKAVEMFRAVADGRIKAI
                     WIMGTNPVVSMPDADFVEAALKACPLVVVSDVVAETDTLPCAHIKLPAAAWGEKDGTV
                     TNSERRISRQRRFLPLPGVARPDWDIICDVAQRMGFADAFSFKSAAEIFAEYAALSAF
                     ENDGARDFDIGAFADIDAVQFDNMVPFQWPRPTKSAPSETRFFADGNFYTADRKGRFI
                     AVTPCVETRTNSKFPFVLNTGRVRDHWHTMTRTAKSQRLSQHLAEPFAEIHPHDAARL
                     GIHDADVVRISSANHFVLVRALLSKRQVRGAVFVPMHWNDQFASRARVDSLVPSTTDP
                     QSGQPAFKHVAVDVARFEADTYGFAVLADRPRDLDADYWALAKCRGGWRLELALAGGA
                     RDWTDFANSLFGCGRDADLLFYQDVTGGQHRFACFEGERLVGALFLAAEPVSVSRDWA
                     VEQLSVSLADPRRRFSIIAARPGAGGFDRGATVCSCFGVGANQITAAIAAGRSSVEAV
                     GQSTQAGTNCGSCRAEIRKIIDANQQLN"
BASE COUNT          833 a         1364 c         1343 g          878 t
ORIGIN      
        1 atcgttctgc ggcatgggtc tgccggcatc tacaagcggc tcgtgctcaa ggacaatcgc
       61 attatcggca cggtgctctt cggcgaaacg ggcgacggcg cctggttcaa cgatctgaaa
      121 aaaaagagcg tcgacatttc cgacatgcgc gagacgctca tcttcggcca gtcttatcag
      181 ggaggtgccc cgctggaccc tatggcggcc gttgcagcct taccggatga cgcagagatc
      241 tgcggctgca acggcgtatg caagagcgcg atcaccagcg cgatcgcagc caaaggcctg
      301 acctcgctcg acgatgttcg cgcccacacc aaagcctccg cctcgtgcgg ctcctgcacc
      361 ggccttgtcg agcaattgct gaagctcagc ctgggcgagg cctataaccc ggctgcggtg
      421 cagccaatgt gcaattgcac cgatctcggc catgatgacg tgcgccgcct catcaaagcg
      481 aaaagtctca agactattcc cgccgtcatg caggcgcttg aatggaaaac ctcctgcggc
      541 tgcgcgaagt gccgcccggc cttgaattat tatctcgtct gcgaatggcc ggatggatat
      601 gccgacgatt atcagtcgcg tttcatcaac gagcgcgcgc acgccaatat ccagaaagat
      661 ggcacctatt ccgtcgtgcc gcgcatgtgg ggcggcgtga cgagcgcggc cgagctgcgc
      721 gccatcgccg atgtggtcga gaaatttgcc atcccaaccg tgaaggtcac cggtggccag
      781 cgcatcgata tgcttggcgt gaagaaagag gatttgcccg ccgtctgggc cgatctgggc
      841 caggccggct tcgtctccgg ccatgcttac gccaaaggcg tccgcacggt gaagacctgc
      901 gttggcacgg attggtgccg cttcggcacg caggattcga cgggtctcgg catccgcatc
      961 gagaaattca tgtgggggtc gtggacgcca gcaaaagtca aaatggcggt ttctggctgc
     1021 ccgcgcaatt gcgccgaatc gacctgtaag gacgtcggcg tgatctgcgt ggactcaggc
     1081 tacgaaatcc atttcgccgg tgcggcgggc ctcgacatca agagcaccga agtcctcggt
     1141 ctcgtgcaga cggaagacga ggctttggag gtgatcgtcg cgcttgtgca gatgtatcgc
     1201 gagcaggcgc gttatctcga acgtatatac aaatggacga aacgcgtcgg cgtcgccgaa
     1261 atccgccgtc agattctcga cgatgcggat aagcgccaag cctattttga ccgtttcgtc
     1321 ttctcgcaga aattcgcgca agtcgatccg tgggaggaac gcgtgtcgcg caagcacaag
     1381 cacgagttca agcccatggc ggttatcgat tttgctccgg cggcggagtg atgatcatga
     1441 actggatcga catcggcacc gtcaccgata ttccccgccg cggcgcgcgt tgcgtggtca
     1501 cgccgcaagg caggatcgca gtctttcgca cgatggacga tcagttcttc gccatggaag
     1561 accgctgccc acataaaggc ggcccactca gccagggcat cgtgcacggc aatgccgtga
     1621 cctgcccgct gcataattgg gtcatctcgc tcgaaactgg catggcgctc ggcgcggata
     1681 agggaagcgt caaaaccatt ccactgcggc gggatggcga aaagctttat ctcgctttgg
     1741 acgcagccat gcggctggcc ggataatgga agccgcggcg accaaaacca cctgccccta
     1801 ttgcggcgtt ggttgcggcg tcatcacgag cgtcgcgggc gacggcagcg tcgcgatccg
     1861 cggcgatacg gagcaccccg cgaattacgg acggctttgc tcgaaggggt cggccctcgg
     1921 cgagacggtc gatctcgacg accggctgct gtatccttcg atccacggcc atcgtgcaag
     1981 ctgggacgaa gcgctcgatc tcgtcgccgg ggcattttcg agcacgattg ccgaacacgg
     2041 cccggactcg gtcgcgttct atatctccgg tcaattgctg acggaggatt attacgtcgc
     2101 caacaagctg atgaaaggct tcatcggcgc ggcgaatatc gacaccaatt cgcggctctg
     2161 catggcttct tccgtcgccg gacatcggcg cgccttcggt gcagacacgg tgccgggaac
     2221 ctacgaagat ctcgagcttg cggacctgat cgtcctcgtc ggctccaatc tcgcctggtg
     2281 ccatcccgtt ctttatcagc gtatcgccgc ggcaatgcag aagcgcccgc agatgaagat
     2341 cgtgctcgtc gatccacgcc ggacgatgac agccgacatt gcagagatgc atctggctgt
     2401 gcgtcctgac ggcgatgtcg cgctcttcaa cgggctgctt gcatggcttg cggaccacga
     2461 cgcgctcgat cgcgcctata tcgccgggca taccagcggc ttcgatgacg cgttcgccgc
     2521 ggcaagcgcc atgtcatttg atgaaatcgc agaacagacc ggcgtcgcgg cggatgaact
     2581 tcgtgccttc tacgcgctct tcgcgcggac cgaaaaggtc gtcaccgtct acagccaggg
     2641 cgtcaaccaa tcctctgccg gcaccgacaa ggtcaatgcg atcatcaatt gccatttcgc
     2701 aacggggcgc attggccggg cgggcatggg gccattttcc gtcacaggcc agcccaatgc
     2761 gatgggcggg cgcgaggtcg gcggccttgc caacatgctt gccgcgcata tggagatcga
     2821 aaacgccgaa catcgcgatt gcttgcaacg cttctggcgc gcaccgacga tggcagagcg
     2881 gccgggactg aaagccgtcg agatgtttcg cgccgtcgcc gacgggcgca tcaaggcgat
     2941 ctggatcatg ggcacgaatc cggtcgtctc catgcccgac gcggatttcg tcgaagccgc
     3001 gctgaaagcc tgccccttgg ttgtcgtgtc ggacgtcgtc gccgagaccg acacgctgcc
     3061 ttgcgcgcat atcaaactgc ctgcggcagc ctggggcgag aaagacggga ccgttaccaa
     3121 ttccgagcgg cggatttcgc gacagcggcg ttttctgccg ctgcccggcg ttgcgcggcc
     3181 ggactgggac atcatctgcg acgtcgccca acgtatgggc tttgccgatg ccttttcttt
     3241 caagtccgca gcagagattt tcgccgaata tgccgcgctc tcggcctttg aaaatgatgg
     3301 cgcgcgggat ttcgatatcg gcgcctttgc cgatatcgac gcggttcaat tcgacaacat
     3361 ggtgcccttc caatggccgc gtccgacaaa gagcgcgcca agcgagacgc gtttttttgc
     3421 cgacggaaat ttctatacgg cggatcgcaa gggccgcttc atcgctgtga cgccctgcgt
     3481 cgagacgcgc acaaattcaa agttcccgtt cgtcttgaac accggacgtg tgcgtgacca
     3541 ttggcatacg atgacgcgca cggcgaagag ccagcgcctg tcgcagcacc tcgccgaacc
     3601 tttcgccgaa atccatccgc acgatgcggc gagactcggc atccacgatg cggatgtcgt
     3661 gcgcatttcc tccgcgaatc attttgtgct ggtgcgcgcg cttctctcga aacggcaggt
     3721 acgcggcgcc gtctttgtcc caatgcattg gaacgaccaa ttcgcatccc gagcgcgggt
     3781 cgatagcctt gtgccctcga ccaccgatcc ccaatccggc cagccggcgt tcaaacatgt
     3841 ggctgtcgat gtcgcacgct ttgaagccga tacctatggc tttgctgtct tggccgatcg
     3901 tcctcgcgat ctcgatgcgg attattgggc actggcgaaa tgtcgtggcg gctggcgcct
     3961 cgaacttgcg ctggcaggtg gcgcgcgcga ctggacggat tttgctaatt cgcttttcgg
     4021 ctgcgggcgc gatgccgatc ttctattcta tcaggacgtg acaggcggtc agcatcgctt
     4081 cgcctgcttt gaaggcgaac gcctcgtcgg cgccttgttt ctcgccgccg agccggtgtc
     4141 cgtctcgcgc gattgggccg tcgaacaatt atcggtgagc cttgccgatc cgcgccgccg
     4201 tttctcgatc atcgcagcgc gacccggcgc cggcggcttt gatcgtggcg ccactgtctg
     4261 ctcgtgtttc ggcgtcggcg caaaccagat caccgccgcc atcgctgctg gccgcagcag
     4321 cgtcgaggcg gttggacaat caacgcaggc cggcaccaat tgcggctcat gccgcgccga
     4381 gatcaggaag atcatcgacg cgaaccagca attgaacc
//
LOCUS       DASWOG010000668         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_364130,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000668 DASWOG010000000
VERSION     DASWOG010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364130"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGB93_13680"
     CDS             complement(<1..480)
                     /locus_tag="VGB93_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0147166.1"
                     /translation="MDFFKRFTFLLCAPGFDADDLEGMRLQAIVAAVEDDGFEVVKAR
                     RVEDAELAVQTDAAIGCMVVDWGKRGLEGKTAALINLMRRRGLEMPIVILVRRKRLED
                     IPVEVLDFIDGYIFLSEETPEFIARNLISRLKQYAGTLKTPFFGALVDYAEEGNQLWT
                     "
     gene            complement(592..2070)
                     /locus_tag="VGB93_13685"
     CDS             complement(592..2070)
                     /locus_tag="VGB93_13685"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY0147167.1"
                     /translation="MAKGLATLNDKSSPVIVWFRQDLRLADNPALHAARQTGQPLICI
                     YIFDDVSHGLRPPGGATKYWLHSALKALDESLRKHGGALHVLRGPAKTLIEEFAVTSR
                     ASTVFWNRRYDEAGRNVDTAIKIAFKERGIAAESFNANLLHEPWTIASKTNTPFKVFT
                     PFWRAARVKGEPDAPLGAPRTLTFHDLPKSIEPVALADLRLEPDKPDWAVGLREAWTP
                     GEAGAHAALKHFLKSGLPVYAAQRDRPDKPATSRLSPFLHFGHISPRQVWHAAQAAMM
                     SGTSKASSTDVEKLFSELGWREFSYHLLFHHPDLATVNFQSRFDALTWRHDAKALRAW
                     QRGLTGYPIVDAGMRELWATGWMHNRVRMVTGSFLIKHLLIDWREGERWFWDTLVDAD
                     AANNAASWQWVAGSGADAAPYFRIFNPILQGEKFDPDGAYVRRWVPELAKLPTTLVHR
                     PWKATQDQLAAAGIRLGETYPRPIVEHDAARKRALTALEAVG"
     gene            2612..2842
                     /locus_tag="VGB93_13690"
     CDS             2612..2842
                     /locus_tag="VGB93_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147168.1"
                     /translation="MRRLQLDHVDCYLIHTPFAFRPGDEQDPRDAQGKVIYDAGVTLA
                     ETWRALERLVDDGRCKADARQMQGNQDIRYRP"
     gene            2796..3272
                     /locus_tag="VGB93_13695"
     CDS             2796..3272
                     /locus_tag="VGB93_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147169.1"
                     /translation="MQGRCKAIRISDIGLERLRETVAIARIKPAVVQVESHPYLPEWD
                     LLDFCRQQGIVVLAFAALGHAMAPRVTEDPVIVAIAQRVRKSPAQVALAWAVQRGTAL
                     LTTSTSREHLQENFDISTLPEDAMQEIRDAITTDVRFNAVVGTGVPGFIPPTNRSK"
     gene            complement(3316..5061)
                     /locus_tag="VGB93_13700"
     CDS             complement(3316..5061)
                     /locus_tag="VGB93_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0147170.1"
                     /translation="MLKRALAATALMLGAGLSFAGTPAAAANACPGGNGGLKLSPGWC
                     ATIFADNVGHARQMVVAPNGVLYINTWSGVYYDNDKPPDGGFLLALKDSKGTGHADIV
                     KRFGDGIAQGSAGGTGIYYYNNALYAEENDKIVRFAFPKDTIAPTDTYKIVLSGMPLG
                     GDHPMHPFIIDKSGNMFIDMGTATNSCQGQNRMQGSPGIKPCTELETRGGTWRYDANK
                     TEQKFSPAQRYATGIRNGEGFSFDAAGRLYVTQHGRDQLWQNWPSLYNPKQSAELPAE
                     LLLQVNKGDDFGWPECYYDGLQKKLVLAPEYGGDGGKTQSVCATKKLPVAFFPAHWAP
                     NDLLIDNDARVPAAYRGGAFLAFHGSWNRAPLPQGGYNVVFQPLKDGKAAGDFVIFAD
                     GFAGQYIEPGRAAFRPSGLAAGPDGAIYISDDIHGRIWRLTYHAAPDAPVAAAPGTEN
                     VIAEANNPLPPEGIHPNAGRADTAGLPLPPGVTRDQLALGERIFHGEVDYGTCAGCHG
                     SDAKGTSQGPNLTTPKKIWSDGSLAGLEKTIADGVPNPMNYPGVMPAKGGAPLTQADV
                     VAVAGYVWALSHQKD"
     gene            complement(5107..6057)
                     /locus_tag="VGB93_13705"
     CDS             complement(5107..6057)
                     /locus_tag="VGB93_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147171.1"
                     /translation="MGRASVFGALTALSIATAKAEPAQIVLPGNNIFPESVTSTADGT
                     LYVGSFAAGGIFRVPPGASQAAVWIKPGTFGTRSLLGVYADEKTQTLWVCSNDLSAIG
                     LPGPSKVPGAWLKGFDLKTGVGKISVRFPGKKNFCNDIVVGNDGSVYVTNSWRPQILK
                     LNSATQKLEVWDSDPLFNPPTKGVGLDGIAFSADGDLYVDTFNEAKLFHIEVKNGKPG
                     IVSRVNPSQSLGLADALRPLGGDAFLMIEDVGKLDRVTFDGNEATIETLKDGLNGPTS
                     VTLRGRTAWVSEGQLQHLSDKPGPKPILPFRLVPVTIPKP"
     gene            6468..7064
                     /locus_tag="VGB93_13710"
     CDS             6468..7064
                     /locus_tag="VGB93_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0147172.1"
                     /translation="MSAISRLRAFFFIILLALPISACGYNQIPTLQENAKAKWAEVQN
                     NYQRRSDLIPNLVATVQGYAKQEKDVLVQVTEARAKATQVHIDSSDLTDPAKLKAFQD
                     AQAQLGGALGHLLAISENYPDLKSNQNFLALQSQLEGTENRIAVSRRDYIEAVRQYNT
                     ALVTFPGVLWASTLFKNSKPMAEFTANDNAQTAPQVKF"
     gene            7087..>7690
                     /locus_tag="VGB93_13715"
     CDS             7087..>7690
                     /locus_tag="VGB93_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0147173.1"
                     /translation="MVSQSAKVSFVWPSARAIFAIAALACVLAFVSVAAFAYNFPPLT
                     GRVVDQANIIPDATRQEITQKLADLEQKSGIQLVVATVNSLEDGDIETYANELFRAWK
                     LGEKDKNNGVLLLVAPKEHKVRIEVSYGLEGTLTDALSKIIIVNAITPSFKKGDFGGG
                     ISRGVDDIITTLTTDSSDWQPKPQLRADQDDNSSAIGWVLI"
BASE COUNT         1514 a         2320 c         2323 g         1533 t
ORIGIN      
        1 cgtccagagc tggttgcctt cctcagcgta atcgacgagg gcaccgaaga acggcgtctt
       61 caacgttccg gcatattgct tgaggcggct gatcagattg cgcgcaatga actccggcgt
      121 ctcttccgag agaaagatat agccgtcgat aaaatcgagc acctcgaccg ggatatcctc
      181 gagacgcttg cggcggacga ggatcacgat cggcatttcg aggccgcggc gacgcatcag
      241 attgatcagc gccgccgtct tgccctcaag tccgcgcttg ccccaatcga ccaccatgca
      301 gccgatcgcg gcatcggtct ggacggccag ttcggcgtcc tcgactcgcc gcgccttgac
      361 gacctcgaac ccgtcatcct ctacggctgc gacgatcgcc tgaaggcgca tgccttcgag
      421 gtcgtcggcg tcgaagccgg gcgcgcagag tagaaacgtg aaacgcttga aaaaatccat
      481 cgccatgccg ccccttgtcc agacgcttgg tcggatcgtt ccatgacaaa cgcatgacag
      541 acttccatga cgcgtcttca cagatcgaag gattgcccga ggccgcgcca atcagcccac
      601 ggcttccaac gccgtcaacg cgcgcttgcg tgctgcatca tgctcgacaa tgggacgcgg
      661 ataagtctcc ccaagccgga taccggcggc ggcgagttga tcttgcgtcg ccttccacgg
      721 tctatggacc agtgtcgttg gcaattttgc gagctccgga acccagcgcc gcacataggc
      781 gccatcgggg tcgaatttct cgccctgcag aatcggattg aaaatgcgga aatagggcgc
      841 agcgtcggca cccgaccccg cgacccattg ccagctcgcg gcattgttgg cggcatcggc
      901 atcgacgagc gtgtcccaaa accagcgttc gccctcgcgc caatcgatga gcagatgttt
      961 gatgagaaac gagcccgtga ccatgcgcac gcgattgtgc atccaacccg tcgcccaaag
     1021 ctcgcgcatg ccggcatcga cgatcggata gccggtcagg ccgcgctgcc acgcacgaag
     1081 tgccttcgcg tcgtgacgcc atgtgagcgc gtcaaagcgg ctctggaaat tcaccgtcgc
     1141 aagatccggg tgatgaaaaa gaaggtgata ggagaattcg cgccagccca gttcggagaa
     1201 gagtttttcg acgtcggtcg aactcgcctt cgacgtcccg ctcatcattg cggcctgcgc
     1261 ggcgtgccag acttgccgcg gcgagatatg gccaaagtgc agaaacggcg acaggcgtga
     1321 cgttgccggt ttatcggggc ggtcgcgctg cgccgcgtag acaggcaggc cgcttttcaa
     1381 aaaatgtttc agcgcggcgt gcgcgccggc ttcgccgggc gtccaggcct cgcgcagacc
     1441 aacagcccaa tcaggcttgt ccggttcgag acgcagatcg gcaagcgcga cgggctcgat
     1501 gcttttcggc aaatcatgga atgtgagcgt tcgtggggcg ccaagcggcg cgtcaggttc
     1561 gcccttcacc cgcgcggcac gccagaacgg tgtgaagacc ttgaaaggcg tgttggtctt
     1621 gctggcaatc gtccagggct cgtggagcag attggcgttg aagctttccg ccgcaatgcc
     1681 gcgttccttg aatgcgatct tgatcgccgt atcgacatta cgtccggctt catcgtagcg
     1741 ccgattccag aagacagtgc ttgcacggct cgtcacagcg aactcttcaa tcagcgtttt
     1801 tgctggaccg cgcagaacgt gcaatgcgcc gccgtgtttt ctcaggctct cgtcgagcgc
     1861 cttgagcgcg ctatgcagcc aatatttcgt cgcgccgccg ggcggccgca ggccgtggct
     1921 tacgtcatcg aaaatgtaga tgcagatgag cggctgcccc gtctgccgcg cggcatgaag
     1981 cgcgggattg tccgcgaggc gcaagtcctg gcggaaccag acgatgacgg gactgctttt
     2041 gtcattcaaa gttgccaatc ctttcgccat ggtctttctt ctgttggcac agcgcccaaa
     2101 tatagccccc ttcggcagca ttacgcttgg gtaaatcgtc ggttttcggg cgcgtttgtc
     2161 atcaaggtga atgcgttccg gggtgtggta gcgggtgaac gaacagactc cgatctggag
     2221 ttgacatcct ctcaatagaa gcgccttccc ggatttccga aagcctccgg cgtcttatcg
     2281 ggccagacaa ggagtggacg catggcgaat ccagacgcac ttcgcgacac gaagattcct
     2341 ctcgccggtg ggtccggtgc gatgccggct ctcgggttcg gcacgctgat ccccgatccc
     2401 gtcgtgaccg ggcaagcgac caaggccgcg ttggaagtcg ggtttcggca tctcgactgt
     2461 tcggagcgct atcgcaacga agccgcagtc ggcgaagcga tgcaggaggt gttcaaggca
     2521 ggggggattc ggcgggagac gtcttcgtca cgacgaacta tggaacaaca accatcgtcc
     2581 cgagcgggtt ctgccggcct acgaggcaag tctgcgacga ttgcaactcg accacgtcga
     2641 ttgctatctc atccacaccc cgtttgcgtt tcggcctggc gatgagcagg acccgagaga
     2701 cgcgcagggc aaggtcatct acgatgccgg cgtgacgctg gcagagacat ggcgggcgct
     2761 cgagcgcctg gtggatgacg gcagatgcaa ggcagatgca aggcagatgc aaggcaatca
     2821 ggatatcaga tatcggcctt gagcggttgc gagaaaccgt tgcaattgcg cgcatcaaac
     2881 ccgcggtggt gcaggttgaa tcgcatccgt atctgcccga gtgggacctg ctcgattttt
     2941 gccggcagca ggggatcgtc gtactcgcat tcgccgcgct cggacatgcg atggcgccaa
     3001 gagtcacgga ggatcctgtc atcgtcgcca tcgcgcaacg cgttcgcaag tccccggcgc
     3061 aagtcgcgct ggcctgggcc gtgcagcggg gcaccgctct cctgactact tcgacctcgc
     3121 gtgagcatct tcaggagaac tttgatatct cgactttgcc ggaagacgcg atgcaagaga
     3181 ttcgggacgc cattacgacc gacgtccggt tcaacgctgt ggtggggacc ggcgtgccgg
     3241 ggttcattcc tccgaccaac cgatctaagt gatggctgtg cctcgtcgcg agccaacgag
     3301 gcacagtgtc agctctcaat ccttttggtg gctcagcgcc cacacatagc ccgcaacggc
     3361 aaccacgtcc gcctgtgtca gcggtgcacc gcccttcgca ggcatgacgc ccggatagtt
     3421 catcggattc ggcacgccgt cggcgattgt cttctcaagc ccggcgaggc tgccgtcgct
     3481 ccagattttc ttcggcgttg tcagattggg gccttgcgac gtgcctttgg cgtcggagcc
     3541 atgacagccg gcacaggtgc cgtaatccac ttcgccgtgg aagatacgtt cgccgagcgc
     3601 aagctgatcg cgcgtcacgc ccggcggcag aggcaagccg gccgtatccg cacgcccggc
     3661 attgggatga atgccttccg gaggcaacgg attattcgcc tcggcaatga cattctcagt
     3721 gccaggcgcc gcggcgactg gtgcgtccgg cgcggcgtga taggtcaggc gccagatgcg
     3781 gccgtggatg tcgtcggaaa tatagatcgc gccgtccggc cctgcagcga ggcccgaagg
     3841 ccggaaggcg gcccggccgg gctcgatgta ttggccggcg aagccatcgg cgaaaatgac
     3901 gaagtcaccc gccgccttgc cgtccttcag cggctggaag acgacgttgt agccgccctg
     3961 cggcagcggc gcgcgattcc acgagccatg gaaggcgagg aaagcaccgc cgcgataggc
     4021 ggccggaacc ctcgcatcat tgtcgatcaa cagatcgttt ggcgcccaat gcgcgggaaa
     4081 gaaagcaacc ggcaatttct ttgtggcgca gacgctctgc gtcttgccgc catcgccgcc
     4141 atattccggc gccagcacga gcttcttctg caggccgtcg taataacatt ccggccaacc
     4201 gaaatcgtcg cccttgttca cctggaggag caattcggcc ggcagttcgg cgctttgctt
     4261 cggattatag agcgacggcc agttttgcca aagctgatcg cggccatgct gcgtcacata
     4321 gaggcgccca gcggcatcga agctgaaacc ttcgccattg cgtatgccag tcgcatagcg
     4381 ctgcgcaggc gagaatttct gctctgtttt attggcgtcg tagcgccagg tgccgccgcg
     4441 cgtttcgagt tccgtgcagg gtttgatgcc gggcgatccc tgcatccggt tctggccctg
     4501 gcagctattc gtcgccgtgc ccatgtcgat gaacatgttg ccggatttgt cgatgatgaa
     4561 cgggtgcatc ggatggtcgc cgccgagcgg catgccggag aggacgatct tgtatgtgtc
     4621 cgtcggcgcg atcgtgtcct tcgggaaggc gaagcgcacg atcttgtcgt tctcttcggc
     4681 gtaaagcgcg ttgttgtaat aatagatgcc agtgccaccg gccgagcctt gcgcaatgcc
     4741 gtcgccgaat cgcttgacga tgtccgcgtg gcccgtcccc ttgctgtcct tcagcgcgag
     4801 gaggaagccg ccgtccggcg gcttgtcatt atcgtaatac acgccactcc aggtgttgat
     4861 gtagagcaca ccattcggtg cgacgaccat ctggcgcgcg tgcccgacat tgtcggcgaa
     4921 gatcgttgcg caccagccag gcgagagttt caggccgcca ttgccgccgg ggcaggcatt
     4981 cgccgctgcc gctggcgtgc cggcaaacga aagtccggcg ccaagcatca gagccgtggc
     5041 cgccagggca cgtttcaaca tcgcaaactc cttatcgcgt cggtcaaaag gaagcgggct
     5101 ttcgcctcaa ggcttcggga tcgtcaccgg cacaagccgg aaggggagaa tgggtttggg
     5161 gccgggttta tcggacagat gctggagctg gccctcagaa acccaggctg ttctgccgcg
     5221 caaggtgacg ctcgtcgggc cattcaaccc atccttcaac gtctcaattg tcgcctcgtt
     5281 accgtcgaag gtaacgcgat caagcttgcc gacgtcttcg atcatcagaa acgcatcgcc
     5341 gccgagcggc cgcaaggcgt ccgccaggcc gagcgattgc gatgggttga cccggctgac
     5401 gatgcccggt ttcccgtttt tcacctcgat atggaagagc ttggcctcgt tgaacgtatc
     5461 gacgtaaagg tcaccgtccg cactgaaggc gatcccgtcg agaccgacac ctttcgtggg
     5521 tggattgaag agcgggtcgc tatcccagac ttcgagtttt tgcgtcgcgg aattgagctt
     5581 gagaatctgt ggtcgccagg agtttgtcac atagaccgag ccgtcgttgc caacgacgat
     5641 atcgttgcag aaattcttct tgccggggaa acggacgctt atttttccga cgccggtctt
     5701 gagatcgaag cccttgagcc aagcgccggg taccttgctc ggcccgggaa ggccaatcgc
     5761 ggaaagatca ttggagcaga cccagagcgt ctgcgtcttt tcgtcggcgt agacaccaag
     5821 caacgagcgc gtgccgaacg ttccgggctt gatccacaca gccgcttggc tcgcgcccgg
     5881 tggaacacgg aagatgccgc ccgccgcaaa gctgccgacg taaagggtgc catccgccgt
     5941 cgacgtcacg ctctcgggga aaatattgtt gccgggcagg acgatctggg caggctcggc
     6001 ctttgcagtg gcaatgctca gcgccgtgag cgcgccgaaa acactggccc gccccaaatt
     6061 cgcgcgctgg tgtgtagcga ctatgtacat tagcttcccg cgtccaatgc agggagcatg
     6121 taggcacaag gcgggcgcac cgccagcctt gtctggtaag tttctaatca gcggccggca
     6181 tgaagaaaac atgacgacag atgcgggtta tctcatatcc gatctgcgcg agtgatcgcc
     6241 cgcatctttg gcaagtcgca tcgaccgcaa tgtcaaacgg cggcgatcat ccggaaaatg
     6301 ccaagtttac ttttcgacgt cttttgtcgc accgcgtgtg agggcgcacc atggtcacat
     6361 gctatagccg gtgtcgcggc cgtgacccaa ttgacacggc gatgcgaacc ttggcttaat
     6421 ccgcgccgca aagttagccg ttcaatcttg atcacgaggc ctttatgatg tctgcgattt
     6481 cgcgtctgcg cgcttttttc ttcatcattt tgctcgcgct tccaatttcg gcctgcggct
     6541 acaatcaaat tccgaccttg caggaaaatg ccaaggccaa atgggcggag gtgcaaaaca
     6601 attatcagcg ccgctccgac ctcattccaa accttgtcgc aaccgttcag ggttacgcca
     6661 agcaggagaa ggatgtgctg gttcaggtga ccgaggcacg cgccaaggcg acgcaagtgc
     6721 atattgattc ctcggatctt accgacccgg caaaactcaa ggccttccag gatgcgcagg
     6781 cgcagcttgg cggcgcgctc ggccatcttc tggcgatcag cgagaattat ccggacttga
     6841 agtcgaacca gaattttctt gcgttgcaat cgcagcttga gggcacggaa aaccgcatcg
     6901 ccgtctcgcg tcgcgattat atcgaggcag tgcggcaata taatacggcg cttgtgacgt
     6961 tcccgggcgt tctctgggcg tcgaccctgt tcaaaaacag caagccgatg gcagaattca
     7021 cggcgaacga caacgcccag accgcgccgc aagtgaaatt ctaactcctg cgccggaggc
     7081 gctggaatgg tttcacaaag cgcaaaagtc tcgtttgtct ggcccagtgc gcgcgcgatt
     7141 ttcgcaatcg ccgcgctcgc ctgcgtcttg gctttcgtca gcgttgcggc cttcgcctat
     7201 aattttccgc cgctcaccgg ccgcgtcgtc gatcaggcca atattattcc cgacgcgacg
     7261 cgtcaggaga tcacgcagaa gctcgccgat ctcgaacaga aatccggcat tcaacttgtc
     7321 gtcgcgacgg tcaattcgct ggaagatggc gacatcgaga cctacgccaa cgaactcttc
     7381 cgcgcctgga aacttggcga gaaagacaag aacaacggcg tgctactgct cgtcgcgccg
     7441 aaggagcaca aagtccggat cgaggtcagc tatggcctcg aaggcacgct caccgatgcg
     7501 ctgtcgaaga tcatcatcgt caatgccatc accccatctt tcaagaaggg cgacttcggc
     7561 ggcggcattt cccgtggcgt cgacgacatc atcacaactc tcacgaccga ctcgtcggac
     7621 tggcagccga agccgcaact gcgggccgat caggatgaca attcaagtgc gatcggttgg
     7681 gtgctgatcg
//
LOCUS       DASWOG010000669         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_71230,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000669 DASWOG010000000
VERSION     DASWOG010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71230"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VGB93_13720"
     CDS             complement(<1..1573)
                     /locus_tag="VGB93_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0147174.1"
                     /translation="MAAGSVSAAEAQQATGAAPALAPVTVTAPVAHHRHVAAKPTKAH
                     LRARTALRHKLRETTTAQSPANPGASAAGQTTGHVPGSDPYANPNAPYQAERFSSSKF
                     SEPIFNTPRSVTVLTQQVLQDENATSLRDIGRTTAGVTLGSGEGGNAFGDRFFIRGFD
                     ARNDIFVDGIRDPGVSIRENFFTEEVEILRGPASSYAGRGTSGGAINIVTKQAEDDDF
                     YSAQSQLGSDETRRFTFDVNKAISPILDVRLDGLIQGAGIAGRDFTTDDRNGLGGALT
                     FRPLSNLTIKADYSHTYLSGLPDFGVPYNLPAETPVTNGTVPRNTYYGIVNRDFTKTT
                     QNIGTIDLQYHVNDVVTLENKMRQEYSILNYIGTIPENPAATVLLPPGAPGGCGGTAS
                     STPTCLSGYTQLNAQSRFETVAVLADDPQVNFDFYTGPVKNAVVFGGDFDHERISINS
                     YTGLTSEGLGSEGLVSSTGAPIVSIYDPTNYLNGLGTPTLGGNPQIYNVDTKAVYLLE
                     TANYNDWLIANAGVRF"
     gene            complement(1782..2033)
                     /locus_tag="VGB93_13725"
     CDS             complement(1782..2033)
                     /locus_tag="VGB93_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147175.1"
                     /translation="MDELNGRMIACQILLTGLIAKVANNSPEPLRFLTEFRDEVRAVI
                     NQIRISGSHDTTRVRQSALATADELFGLMKPPSTVEAED"
BASE COUNT          402 a          593 c          661 g          467 t
ORIGIN      
        1 cgaaacgcac gccggcgttg gcgatcagcc aatcgttgta attggcggtt tcgagcagat
       61 agaccgcctt ggtatcgaca ttgtagattt gcgggttgcc gccgagcgtg ggcgtaccga
      121 ggccgttaag gtaatttgtc gggtcataga tgctgacgat cggggcgccg gtcgatgaga
      181 cgagaccttc cgaaccgaga ccctcggagg tcaggcccgt atagctgttg atgctgatac
      241 gttcgtggtc gaaatcaccg ccgaagacga cagcgttttt gaccgggccg gtgtagaagt
      301 cgaagttcac ctgcgggtca tccgcaagca cggcgaccgt ttcaaaacgg ctttgcgcat
      361 tgagctgtgt gtaaccggag aggcaagtcg gagtcgaact cgccgttcct ccgcaaccgc
      421 ccggcgcgcc cggcggcaag agcacggtgg cggcggggtt ctccggaatc gtcccgatat
      481 aattcaggat cgagtattcc tgacgcatct tgttttcaag cgtgaccacg tcgttgacat
      541 gatattgcag atcgatcgtg ccgatgttct gcgtcgtctt ggtgaaatcg cgattgacga
      601 tgccgtaata ggtattacgg ggcacggtgc cgttggtgac cggcgtttca gcgggcagat
      661 tgtagggtac gccgaagtcg ggcagacccg agagatacgt atgcgaataa tctgccttga
      721 tggtcaggtt cgacagcggc cggaaagtca gcgcgccgcc gagaccgttg cgatcgtctg
      781 tggtgaaatc gcgtccggcg atgcccgcgc cctggatcag gccgtcgagg cgcacgtcaa
      841 ggatcgggct gatcgccttg ttgacgtcga aggtgaaacg gcgcgtttcg tcgctgccaa
      901 gttgcgactg tgccgagtaa aagtcatcgt cctcagcctg tttggtgacg atgttgatag
      961 cgccgccgga agtcccgcgg ccagcatagc tcgaagccgg tccgcgcaga atctcgactt
     1021 cttccgtaaa gaaattctcg cgaatgctga caccagggtc gcggatgccg tcaacgaaaa
     1081 tgtcgttgcg ggcgtcaaac ccgcggatga agaatcgatc gccgaaggcg ttgccgcctt
     1141 cgccggagcc gagggtcaca cccgccgttg tgcggccaat atcgcggagc gacgtagcgt
     1201 tttcgtcctg cagcacctgt tgcgtaagaa cggtcaccga gcgcggtgta ttgaagatcg
     1261 gctcgctgaa cttgctcgaa gagaagcgct cggcctgata gggcgcgttc ggattggcgt
     1321 agggatcgct gccagggaca tggcccgtcg tctggcccgc cgcgctggcg ccgggattgg
     1381 cgggtgattg cgccgtcgtc gtttcgcgca gcttgtggcg cagcgctgtg cgcgcccgca
     1441 gatgcgcttt tgtcggcttg gccgcgacgt gacggtgatg tgccacaggc gcggtgaccg
     1501 tgacgggcgc gagcgcgggt gcggcgccag tcgcctgttg ggcttccgcc gcgctcaccg
     1561 atcctgccgc catcagaaag gccgttgtaa gtgtcgcgcc ctctttgaca tcagaccgct
     1621 ttcttgtccg ggccccagaa tcgcgcatgc gtaaagaacc cggaactttc actccgctca
     1681 tttcagcccc cattcccgcg cttgacgcgc gccccagata aatttcaaac ttcggttgtt
     1741 gtcgttttct tattcgtcgc ttcgattgat gcttatcgaa atcagtcttc cgcttcgacg
     1801 gtgcttggcg gtttcatcaa tccgaacaac tcgtcggctg tcgccaaagc tgattgccgg
     1861 acccgcgttg tgtcgtggga gcccgaaatc cgaatctggt tgatgaccgc gcgaacctca
     1921 tcgcggaatt ccgtcagaaa ccgcaaaggc tcgggcgaat tattcgcgac tttagcgatg
     1981 agccctgtca gaagaatctg acatgcgatc atccgcccat tgagttcgtc caaagcccca
     2041 cgtccaatac ttagaagaat tctagaaatg acttttttgt caggagcgtt ttgactcggc
     2101 tggtgaggtt taacaacacg cgg
//
LOCUS       DASWOG010000670         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_237587,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000670 DASWOG010000000
VERSION     DASWOG010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237587"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..817)
                     /locus_tag="VGB93_13730"
     CDS             complement(143..817)
                     /locus_tag="VGB93_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147176.1"
                     /translation="MMPILLTLVALVFLPWFYAKGARRARERRGEFFANSTRLFETYR
                     VTQQGMNYPVLEARYQGLPVRLEPVLDNMGWRKLPSLWLKASLLVPNATRGTLDFLVR
                     PQGVEFYSPSYDFHEHLRVPASWPQNALLATLPGAAVPSLDKLAPHIEVFDDLKTKEL
                     VVTPHGIRLVYQAAQAERADYLVLRQATFTEPRVDPALVRALLDRLIAIAATLDAVPT
                     SEAEAA"
     gene            complement(821..>1897)
                     /locus_tag="VGB93_13735"
     CDS             complement(821..>1897)
                     /locus_tag="VGB93_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY0147177.1"
                     /translation="YTMLNASDIQVAGQLVQIGAQQLGHDIPWQPFTVLCLAVLTWLN
                     YRGVFATLTVNFIITAAAFSTIIILFIGVQPWNPTAMLLHKDLLTNLPYGWLGVIAAL
                     HFGIWYYLGIEGTCQAAEEVRSPGRALPLGTMSGMITLLIAAAITWYVTCGLLPWQYI
                     GWDNELAPLYDTARVTGSSFLTVALFIGTLLSAIASANGCINDSARAWFSMGRDRYLP
                     TWFGAVHPKYRTPFRSILFLTPIAVIFALYIPLAEIITFSILSGLLNYTFMPLNMWAF
                     RRKWPLGTIRRGFEHPFHPVPAFTLMFLCITTFFAVYLGYGRQLLAMMAFYIIASVWF
                     AFYRYRYVRRGAQFTMSWPKPIGY"
BASE COUNT          443 a          532 c          606 g          316 t
ORIGIN      
        1 gccatcagga tcatgaacat ctgcatggtg aagccacgtt tcggccggcc gacgagcacg
       61 taccccatac cgggcaggat gaccgcaaca gccagaacga aatagggatt gggatcggcc
      121 aaaagcgcgc gcaatttatt catcacgctg cctcggcttc gctcgttggc acggcgtcga
      181 gggtcgcggc gatggcgatc aaccggtcga gtagcgcccg caccaaagcg gggtcgacac
      241 gcggctccgt gaaagttgcc tggcgcagca ccagatagtc ggcgcgttcg gcctgcgctg
      301 cctgatagac aagccgaata ccgtgcggcg tcacgacgag ctctttagtc ttcaaatcgt
      361 cgaagacttc aatgtgtggt gcgagtttgt cgagcgatgg aaccgccgcg ccgggcaaag
      421 tcgcgagcag ggcgttctgt ggccagcttg cgggcacccg caggtgttca tgaaaatcgt
      481 aagacggcga atagaattcg acgccttgcg gccgcacgag aaaatcgagt gtgccacgcg
      541 tcgcgttcgg caccagcagc gacgctttga gccaaagcga cggcaacttg cgccagccca
      601 tattgtcgag cacgggctcg aggcgcacgg gcaaaccttg atagcgcgcc tcgaggaccg
      661 gatagttcat gccctgctgc gtcacccggt atgtttcaaa aagccgcgtg ctgttggcga
      721 aaaattcgcc gcgacgttcg cgcgcgcgcc ttgcgccctt ggcataaaac cagggaagaa
      781 agacgagcgc caccaaagtg agaaggatgg gcatcatact tcaatagcca atcggcttgg
      841 gccaggacat cgtgaactgt gcgccgcggc gcacgtaacg atagcggtag aacgcgaacc
      901 agactgaggc gatgatgtag aaggccatca tcgcgagcag ctggcgcccg tagccgaggt
      961 agacggcgaa gaacgtcgtg atgcaaagga acatcaaggt aaacgccggc acgggatgga
     1021 aggggtgctc aaagccgcgg cgaatggtgc caagcggcca cttgcgccgg aacgcccaca
     1081 tgttgagcgg catgaacgtg tagttcaaca ggccggacaa gatcgaaaag gtgatgatct
     1141 ccgcgagggg aatatagagg gcgaaaatca ccgcgatcgg cgtcaggaac aggatcgatc
     1201 ggaacggcgt ccgatatttc ggatgcacgg cgccaaacca tgtcggtaga tatcggtcgc
     1261 ggcccatcga gaaccaggcg cgggccgagt cgttgatgca gccgttggcc gacgcgatcg
     1321 ctgatagcaa agtcccgatg aagagcgcca cggtgaggaa gctgctgccg gtcacgcggg
     1381 ccgtgtcgta aagcggcgcg agctcgttat cccagccgat atattgccaa ggcaggaggc
     1441 cgcatgtcac ataccaggtg atcgcggctg cgatcagcaa cgtgatcata ccactcatcg
     1501 tgccgagtgg cagagcgcgc cccggcgaac ggacttcttc ggcggcctgg cacgtgcctt
     1561 cgatgccgag atagtaccag atcccgaagt gcagcgcagc gatgacgccc agccagccgt
     1621 aaggcaggtt ggtaagcaga tccttatgca gcaacatcgc cgtggggttc cacggttgca
     1681 cgccgatgaa aaggatgatg atcgttgaaa aggccgcggc ggtaatgatg aagttcactg
     1741 tcagcgtcgc gaagacaccg cgataattga gccatgtcaa caccgcgaga cagagcactg
     1801 tgaagggctg ccaggggatg tcgtgtccca attgctgcgc accgatctgc accaactggc
     1861 ccgcgacctg aatatcgctg gcgttcaaca tcgtgta
//
LOCUS       DASWOG010000671        11758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_329045,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000671 DASWOG010000000
VERSION     DASWOG010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329045"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1139)
                     /locus_tag="VGB93_13740"
     CDS             complement(213..1139)
                     /locus_tag="VGB93_13740"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0147178.1"
                     /translation="MSRMKIATKVFPGPQNIGLFTAEALGLFARRGIDANIQITIGSD
                     EQRDSLRNGATQVIHSAVDNAVYMKAIDGIDVVIVAGGGTGMNDLIARPEIHSYEDIR
                     GKTVVVDAAMTAYAFLLYSILAKHGLERGSYNVLPLGGAPQRLRAMQENPDYVAAMLN
                     PPCSLQAQAEGYRSFGSAVSILGPYQGDGVFALRDWAESHSSTLVQYLAAFIDGTRWA
                     LAPENHNAATEILQGRLNLTPGIAALSLKTALGQNGGLAPGARLDLDGFMNTLAIRES
                     VVGTWQGAVPEATSFLDMHYYDRAILELGARP"
     gene            complement(1139..2146)
                     /locus_tag="VGB93_13745"
     CDS             complement(1139..2146)
                     /locus_tag="VGB93_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0147179.1"
                     /translation="MTQLTLSLACAPYDRVQPLFDGRVKIAGCVVNGHPMPADEAFSR
                     AYTTQDFDITELSASSHLLTSARGDSPYVGIPAFVSRSFRHSSFYIRTDRGVAGPADL
                     KEKRIGVPQYQQTAALWARGLLADEYGVQASEINWRNGGLDAPGGGERVPLALHGIDL
                     QPIPAGATLSAMLATGDLDAVIAARPPTCFLNGVPNVARLFPNFREAEENYFRKTGLF
                     PVMHLIGIRRTLVETHPWLAAAVFDAFAAAREIAMSQLRYQGAFYAMLPWLPDDLARA
                     QAVMGSDFWPYGVAANRRELDAMQRWAVEQGLAKSEPRLDQIFASEFLEGDAAHEGSA
                     T"
     gene            complement(2146..3093)
                     /locus_tag="VGB93_13750"
     CDS             complement(2146..3093)
                     /locus_tag="VGB93_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0147180.1"
                     /translation="MNNIPSKAQTGPSLVPVAENVYAWIGVNGDSNAGVVTTPQGLIV
                     IDAQQTEAQGRNFRKTIETALGKPVIRLVNSHFHLDHVAGNIAFADVPILAHERTGQI
                     LREMLGKEPPGGWQISDTFTKLKLFFGSNIEDLLAADKAGVEWFMRRVSGPGYDTMTV
                     RAPTESFADKFVFDVPNDELRCEYWGPAHCDGDLIIHLRNSGVVFLGDLLFVGRFPWF
                     GDCDLGGWVKRLDQVLQMDVKTVIPGHGQPSTLQEVAAFHALLAATYDAVRETVEAGL
                     SEDAAVAKVFLPQYASMPRYEAWMPFNVRATYRALRGGG"
     gene            complement(3090..4502)
                     /locus_tag="VGB93_13755"
     CDS             complement(3090..4502)
                     /locus_tag="VGB93_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0147181.1"
                     /translation="MAESKKFDLLVRGGTLVVPGVGTFPADVGISKGQIVAIGDDLRP
                     AEDILDARGKLVLPGIFDPHTHIGNERSFEEEAETETRAAILGGVTTIGIFLRSLKDS
                     YFDHLPAFRAAMDTRSYVDSVFHPQIFTPEQIKEIPGYAEEYGIRSFKFYMSGIPGIV
                     NSITDDLLLEGFRTVAGLGPDTVACVHCETGALVDQARKSLKERKPEGTLADWAEAHP
                     PEAEALAIQTALYLAKIANAHLYVVHVSSRDGLNMVRRARREGARVTAETMTPYLSLD
                     SSDENGFLVKMVPPIRAKEHGDALWGGLTEGTINTLGTDNTSRTRGTKQPGAGLHGAR
                     PGYPVLGTHLPSMLHYGRLRGVPLEMLIDATTRAPAKVYGIYPRKGTISIGSDADLVV
                     VDPDLERVVRAEDLNGMSDFSPFEGKTLRGWPIATIKAGEIIARDGRIVATANGRYLP
                     RKPAGHAPVVPWLSGAQYAS"
     gene            complement(4516..5544)
                     /locus_tag="VGB93_13760"
     CDS             complement(4516..5544)
                     /locus_tag="VGB93_13760"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0147182.1"
                     /translation="MTHEKNFRATPLSRRGLLQTALGAGAALAAGASPLRAAPLKKIT
                     FQLDWIAYGRHVPYYVALEKGYFAQAGLDVNIEQGTGAMPGFRYLAADRAQFVFQDVG
                     SMIAARVRANLKMKAVSCVYQKAPHTAFFIKGKGIAAPKDLEGKKLGFSAGNSPKIMF
                     PAFAEANHIDQSKLNWLAADPNSLNSLLLNHQVDVILTYLFTLPVLQKSAQNGEQIGT
                     FVYSDFGANFYANGIVAMEDYIAANPDIVRGFVKAVKQGFEYTLAHPQDSVALMKKHQ
                     PQLNADTALKELPILARLSTSDDTKKHGFGVMTESKMQETASLIAKYMDLKSEVPIRD
                     LYTNDFLS"
     gene            complement(5579..6394)
                     /locus_tag="VGB93_13765"
     CDS             complement(5579..6394)
                     /locus_tag="VGB93_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0147183.1"
                     /translation="MAIDAAPEPAKFMRKFEIPGFVYSVGAIAALLLIWEALVATGII
                     SSFLLPAPSQIFRSLADNFSLIMSMSVATTAEFLLGFAMAVLVGLPLGALIVYSRPIR
                     LTIYPLLVAFQSIPKPAIAPIMIVWLGTGLTSKAVIAFAIAFFPIVVDTAIGLRSAQE
                     ETVHLVRSMGGNAFDIFRYVRFPNAVPAIFGGLKIASTLAVVGAIVGEFVSADKGLGY
                     LVLVANGNLDTRLVFACVFMLTLLGLAFYFAIEGLERAILRGRVRTQAFESDH"
     gene            complement(6398..7213)
                     /locus_tag="VGB93_13770"
     CDS             complement(6398..7213)
                     /locus_tag="VGB93_13770"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0147184.1"
                     /translation="MSFVAEHTTSLAAADPLIRVQDVVKAYPDQSRSTLAVDRVSLDV
                     MPGEFVSIVGPSGCGKSTLMMMVSGLVPYTGGEIRIGGTRVATPYTDVGIVFQRDALL
                     EWRTILDNVLLQIDVRRLKRRDYLDKARALLARAGLGSFENAYPRELSGGMRQRASMC
                     RALVHDPSLILMDEPFGALDALTREQMNVDLQKIWLEQKKTVLFITHSIWEAVFLADR
                     VIVMSPRPGRIAKEIKIDIPRPRRLAIRETAQFGEYSAQIREQFQSYGMLVED"
     gene            7401..8912
                     /locus_tag="VGB93_13775"
     CDS             7401..8912
                     /locus_tag="VGB93_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0147185.1"
                     /translation="MANMRRNPRAQAPTEINVEVTRHVGGPLFVQLREQIQVLVARGA
                     LTPGDRLPPVRALAAQLNVNQITVARAYSELSDLGVVEGRRGGGTFVCERAVVVAKKP
                     PDRNLEGAFRPLLADRLFELSRAPGVIAFTSNYPFVGTDMIEEFRACVRLAAGRDFEN
                     CFHYEPPLGRLGTRQEISRYLDRRGMDVDPEKIVVTSGAQQAIDLAVRSVVEPGTPVI
                     VERPAYYGALNALRGAGAQILEAPLDGDGIDLQSVETHLVEHQVRLIYVNPTFQNPTG
                     QTMSETKRRDLLALARRFDAVILEDDHSSDWRFAGRPVPAIAALAGPEHRVFYAYSFG
                     KTLLPGQRFGFLVVPPGLRRVVLNRKAALDLHGPSFLQEALAHFLLRGNHEVFFEGAR
                     TSYGERQKSLRQRLLARMPPGTKISAPDGGLSFWITLPEGAEVSELYFRAVRRGVAFV
                     PGEAFFATKPDIEALRVSFGLVPETEISEGVDRLCAVVGDLLTPRPERSVILT"
     gene            8942..9346
                     /locus_tag="VGB93_13780"
     CDS             8942..9346
                     /locus_tag="VGB93_13780"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0147186.1"
                     /translation="MTTKTIIQPRGMAEAVGPFSRAILIGDQLHIAGTTALSHVSGDY
                     YDRKVPETIEEQTRLTLDNIRLCVEAAGGTMADIFKVVVMLKNPSEYKRMNKVRAEYF
                     PKDPPISTCFRAEVMRDDILVEIEAVALINKK"
     gene            complement(9383..11026)
                     /locus_tag="VGB93_13785"
     CDS             complement(9383..11026)
                     /locus_tag="VGB93_13785"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEY0147187.1"
                     /translation="MTKVRIAHIAGPTATIQNTPPLVTSNKAREKQGLPPRLNPDSSQ
                     PAFDPLRPQRLAAPARIYVEQFSAHPLESDAANLYAAPDGYVGEDGVFHRERQNASDK
                     PVYEIELRPEDGLYPLPYMAFQADGSPWEQECAAPDVPKEQARQTFFPDGSRLFEEID
                     RLSVGGDGMANPLSSVATVSFYRAAPSGGAAPERRGRDFFAYQPRHLGAQTPRPALAT
                     ITNTVQRAANSGMHDGLIWTQGSPQVEETSYWFNLLIDTTLPICCNAAQRTHGQMSND
                     GPKNIADSTRFIASRAWADAEGRNKCGTVVIQEQQIFAARDVAKGDARPGGYIVTGGS
                     GGILGHVGHFGVVSLTYLPAYKHTYLSEVNINRLPAVTRAVRKADNGSIESFEIAVKD
                     AQNGLLADAIPAVSIVKDGAYSTLDYGDDPALETDLHASIAHKLSLNRLTGFVIEGLT
                     PYGHSASTAREKVMLQAVYSGLPVCRVGRGGQGFADPHPYQIAASNLTATKARLLLMA
                     CLMKFGSLPPAKDPKNPTGDELAKIRDAIATYQAVFLSH"
     gene            complement(11053..>11758)
                     /locus_tag="VGB93_13790"
     CDS             complement(11053..>11758)
                     /locus_tag="VGB93_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147188.1"
                     /translation="GVPPRQGDIMQGASVLFSEMTPPPGLEQRFHNWYDEEHIPLRMD
                     VPGFVSAQRYRDLSENAKGYLAVYEMTHPAVMKTPAYQEVKTKPSETTREMLGTVSGF
                     TRYIAAETSVKRQPNDSGVSALDAPLLFAVWFEVPESALADFDAWYELDHVPLLMGCK
                     DWSMVRRFRVVDGEPTKANLLALHYLADKAALESPERAAARKTPWRDRLAALPWFSGS
                     YKLFEKHGPRQIGRLR"
BASE COUNT         2342 a         3401 c         3713 g         2302 t
ORIGIN      
        1 gcggcggccg tgtgcgtacg tgccagccaa tcggtaccgg aagacatcag cagatcgaga
       61 ccggtcggaa ggtcgggcgc ggcgatgcgc aggtcgtaaa tgccatcatc tttacgcacc
      121 gcaatcgtgg cgcggccatc gcggcttaga gagagcaggc gcattttgta ccttccgttg
      181 aaacgatgtt gttggctatg cgtgcgtcgg agctatggtc tcgcgccaag ctcaaggatc
      241 gcacggtcgt aataatgcat gtcgagaaaa gacgtggcct cgggcacagc cccttgccac
      301 gtcccgacga ccgactcgcg aatggcgagc gtattcataa agccgtcgag atcgagccgt
      361 gcgcccggcg caagaccgcc attttgaccg agcgcggttt tcaatgagag cgccgcgatt
      421 ccgggcgtga ggttcagccg accctgaaga atttccgtcg ctgcattatg attttcaggc
      481 gccagcgccc agcgcgttcc gtcgatgaac gcagcgagat attggacaag cgtggagctg
      541 tggctttcgg cccaatcgcg tagggcgaag acaccatcgc cttgataggg tccaagaatg
      601 ctgaccgcag agccgaaact gcgatagcct tcggcctgtg cctgcaagct gcacggcggg
      661 ttgagcatcg ccgcaacgta atccggattt tcctgcatcg ctcgcaagcg ttgcggagcg
      721 cccccgaggg gcaacacgtt ataagagccg cgctccaacc cgtgctttgc caggatcgaa
      781 tagagcagga aggcataggc tgtcatggcg gcatcgacga caacggtctt gccgcgaata
      841 tcctcataag aatggatttc aggccgcgcg atcaggtcgt tcatcccggt cccaccgccg
      901 gcgacgatta cgacatcgat cccgtcgatc gccttcatgt aaacggcgtt gtcgacggcg
      961 gagtggatca cctgcgtcgc gccgttgcgc aggctgtcac gctgctcgtc ggagccgatt
     1021 gtgatctgaa tattggcgtc gatccctcgc cgcgcaaaaa ggcccagcgc ctcggcggtg
     1081 aagaggccga tattttgcgg gcccggaaag accttggttg cgattttcat tcgactcatt
     1141 acgtggcgct cccttcgtgc gcggcatcac cttcgagaaa ctcggacgcg aaaatctggt
     1201 cgagccttgg ttcgctcttg gccagccctt gttcgacggc ccagcgctgc atcgcgtcga
     1261 gctcgcgccg gttcgccgcg acgccatacg gccagaaatc ggatcccatc acggcctgcg
     1321 cgcgcgcgag gtcgtcgggc agccacggca gcatcgcgta gaacgcgccc tgatagcgca
     1381 attgactcat tgcgatttcg cgcgcggccg caaaagcgtc aaaaacggcg gcggcgagcc
     1441 aagggtgtgt ttcgaccaac gtgcgccgga tgccgatgag gtgcatcacc ggaaacagtc
     1501 cggtcttgcg gaaataattt tcttcagcct cgcgaaagtt aggaaatagg cgggcaacat
     1561 tcggtacgcc attgaggaag caggtaggcg gacgcgcggc gatcacggcg tccagatctc
     1621 ctgtggcaag cattgccgac aaagtggcgc ccgctgggat cggttgcaga tcaattccgt
     1681 gaagcgcgag cggcacacgc tcgccaccgc ctggcgcgtc gaggccacca ttgcgccagt
     1741 ttatctcgct tgcctgcacg ccatattcgt cggcgaggag tccacgcgcc cataaagccg
     1801 ccgtctgctg atattgcgga acgccgatgc gcttctcttt aaggtctgcc ggcccggcga
     1861 cgccgcgatc ggtgcggatg tagaatgacg aatggcgaaa gctgcgcgag acaaaagccg
     1921 ggatgccgac atagggcgag tcgccacgcg cgctcgtcag gagatgcgaa ctggccgaga
     1981 gttcggtgat gtcgaaatcc tgcgtcgtgt aggcacgcga aaatgcctcg tccgccggca
     2041 ttggatgccc attgacgacg cagcctgcaa ttttgacgcg gccgtcgaag agcggctgca
     2101 cgcggtcata aggcgcacag gcgagtgata acgtgagttg ggtcatcagc cgccaccacg
     2161 tagcgcgcga taggtcgcac gcacgttgaa cggcatccac gcttcatagc ggggcatgga
     2221 tgcatattgc ggaaggaaga ctttcgccac tgcggcgtct tccgaaaggc ccgcctcgac
     2281 tgtctcgcgg acagcgtcat acgtcgcagc gaggagcgca tggaacgcgg cgacctcttg
     2341 caacgtcgag ggctggccgt gaccgggaat cacggtcttc acgtccattt ggagaacctg
     2401 atcgagccgc ttcacccaac cgccgaggtc gcaatcgccg aaccacggaa agcggccgac
     2461 gaacaggagg tccccgagaa agacgacgcc agagttgcgc aaatggatga tgaggtcgcc
     2521 gtcgcaatgc gcgggacccc aatattcgca gcgcaattca tcgttcggaa cgtcgaacac
     2581 gaatttgtcg gcgaagcttt cggtcggcgc gcggaccgtc atggtgtcat agccggggcc
     2641 gctgacacgc cgcatgaacc attcgacgcc ggccttatca gcagcgagca ggtcttcgat
     2701 attcgaacca aaaaacaatt tcagcttggt gaaggtgtcg ctgatctgcc agcctccggg
     2761 cggctccttg cctaacatct cgcgcagaat ctggccggtg cgttcgtgtg ccagaatggg
     2821 cacatcggcg aaagcgatgt tgcccgcgac gtggtcgaga tgaaaatgcg aattgaccag
     2881 tcggataact ggtttgccca acgccgtctc gatggtcttg cggaaattgc gtccctgcgc
     2941 ttcggtttgc tgggcgtcga tgacgatcag gccctgcggg gtcgttacga cgcctgcgtt
     3001 ggaatcgccg ttcacgccaa tccaggcgta aacgttctct gcgaccggca cgagagacgg
     3061 gccggtttgc gccttggaag gaatgttgtt catgatgcgt attgagcgcc gctgagccag
     3121 ggaacgacag gcgcatggcc tgcgggtttg cgcggtaggt aacggccgtt ggccgtcgcg
     3181 acgatgcggc cgtcgcgggc gatgatttcg ccggccttga tcgtggcgat cggccagccg
     3241 cgcaatgtct tgccttcgaa cggcgagaag tcggacatgc cgttcaggtc ttcggcacgc
     3301 accacgcgtt cgaggtccgg atcgacgacg acaagatcag cgtcggagcc gatcgagatc
     3361 gtgcccttgc gcggatagat gccatagacc ttggccggcg cgcgcgtcgt ggcgtcgatc
     3421 agcatttcga gcgggacgcc ccgcagccgg ccatagtgca gcatcgacgg caaatgcgtg
     3481 ccgaggacgg gatagccggg ccgcgcgcca tgcaatccgg cgcccggctg cttcgtgccg
     3541 cgtgtgcgtg atgtgttgtc ggtgcctagc gtattgatcg tcccctcggt aagaccgccc
     3601 catagcgcgt cgccgtgctc tttcgcccgg atgggcggga ccattttgac gagaaagcca
     3661 ttctcatcgc tgctatcgag gctgagatag ggcgtcatcg tctcggccgt gacgcgcgct
     3721 ccttcgcggc gtgcccggcg caccatattg agtccatccc ggctcgaaac atggactacg
     3781 tagagatgcg cgttcgcgat cttggcgagg tagagcgcgg tctggatcgc taaagcttct
     3841 gcctcgggcg gatgcgcctc ggcccaatcc gccagtgtcc cttccggctt gcgttctttg
     3901 agggacttac gcgcctgatc gacgagcgca cccgtctcgc aatgaacgca ggcgactgta
     3961 tccggcccaa gcccggcaac cgtgcggaag ccttcgagaa gtaggtcgtc ggtgatcgaa
     4021 ttcacgatgc ccggtatgcc ggacatataa aacttgaagg agcgtatgcc gtattcttcg
     4081 gcgtagccag gaatttcctt gatctgctcg ggcgtgaaga tctgcggatg gaagacagaa
     4141 tcgacatagc tgcgcgtatc catcgccgcc ctaaaggccg gcagatgatc gaaataggaa
     4201 tctttcagac tgcggaggaa gatgccgatg gtcgtgacgc cgccgagaat tgcagcgcgc
     4261 gtttctgtct ccgcctcttc ttcgaaggaa cgctcgttgc cgatgtgcgt atgcggatcg
     4321 aagatgccgg gcagaacaag cttgccgcgc gcgtcgagaa tgtcctccgc gggtctgaga
     4381 tcatcgccaa tggcgacaat ctgacccttg gatatgccga catcggcggg gaatgtcccg
     4441 acgcccggca cgaccagcgt gccgccgcgg acaagcaaat cgaacttttt actctcggcc
     4501 acccgtccgc tccgttcagc tcaagaaatc gttggtgtag agatcgcgga tcggaacttc
     4561 ggatttcaaa tccatgtatt tcgcgatgag gctggcggtc tcctgcatct tgctctcggt
     4621 catgacgccg aagccatgct ttttggtgtc gtcgctcgtg ctgagccgcg caagaattgg
     4681 cagttccttg agcgccgtat ccgcgttgag ttgcggctga tgttttttca tcagggcgac
     4741 cgagtcctgt ggatgggcga gggtgtattc gaatccctgt ttcacggctt tgacgaatcc
     4801 gcgaacgata tccggattgg cggcgatgta atcttccatc gcgacgatgc cgttggcata
     4861 gaaattcgca ccaaagtccg aatagacgaa ggtgccaatc tgctcgccat tctgcgccga
     4921 tttttgaagc accggcaggg tgaaaagata ggtcaggatg acgtcaacct gatggttgag
     4981 cagaagcgag ttcaacgagt tcggatcggc ggcaagccaa ttgagcttcg actggtcgat
     5041 gtgattggcc tcggcgaacg ccgggaacat gattttgggt gaattgccgg ccgaaaagcc
     5101 gagctttttg ccttcaaggt ctttcggggc tgcgatgccc tttcccttga tgaagaaggc
     5161 tgtgtggggc gccttctggt aaacgcacga gacggctttc attttgaggt tggcgcgaac
     5221 gcgggcggcg atcattgaac caacgtcttg aaagacgaat tgcgcgcggt cggcggcgag
     5281 ataacgaaag cccggcatgg cgccggtgcc ttgttcgata ttcacgtcga ggcctgcttg
     5341 ggcgaagtag cccttttcca acgcgacgta atacggaacg tgacgtccgt aggcgatcca
     5401 atcaagctgg aaggtaattt tcttcaacgg cgccgcgcgc agcggacttg cgccagctgc
     5461 gagcgccgct ccggcgccca gcgccgtctg caagaggccg cgccgggaga gcggcgttgc
     5521 ccggaaattc ttttcgtggg tcacggtcag gtccttctgg aaatggggca gcgatggatc
     5581 aatggtcgct ctcgaacgcc tgagttcgga cgcggccacg caggatcgcg cgctcgaggc
     5641 cttcgatggc gaaataaaag gcgagcccga gaagcgtcag catgaagacg caggcgaata
     5701 caagccgcgt atcgaggttg ccgttggcga ccaggacgag atagccgagg cctttatcgg
     5761 cgctgacaaa ttcgccgacg atggcaccga cgacagccag cgtgctggcg attttcaggc
     5821 cgccaaaaat cgcgggcacg gcattcggaa agcgcacata gcggaaaata tcgaacgcgt
     5881 tgccgcccat ggaccggacg agatgtacgg tctcttcttg tgcggaacgt agcccaatcg
     5941 ccgtatcgac cacgatgggg aagaaggcga tggcaaaggc aatgaccgct ttggacgtga
     6001 gacccgtgcc gagccagacg atcataatag gtgcgatcgc gggcttcgga atgctttgga
     6061 aagcgacgag cagcggatag atcgtcagcc gtatcggccg cgaatagacg atgagcgcgc
     6121 caagcggcag cccgacgagg acagccatgg caaagccaag cagaaattca gcggtcgtcg
     6181 ccacgctcat cgacatgatg agcgagaaat tgtcggcaag agaccggaag atttgcgagg
     6241 gcgcgggcaa gagaaatgag gagatgattc cggtggcgac aagcgcctcc catatgagca
     6301 gcaaggcggc gatggcaccg actgaataga caaatccggg aatttcaaac ttgcgcatga
     6361 actttgcggg ttccggcgct gcgtcgatgg ccaccactta atcctccacc agcatgccgt
     6421 atgattgaaa ctgttcgcgg atctgcgccg aatattcacc gaattgggcg gtctcgcgta
     6481 tggcgaggcg gcgcgggcga ggaatatcga tcttgatttc cttggcgatg cggccaggac
     6541 ggggactcat gacgatcaca cggtcggcga ggaagacggc ctcccagatg ctgtgcgtga
     6601 tgaagagcac cgtcttcttt tgttcgagcc agattttctg cagatcgaca ttcatctgct
     6661 cacgcgtcag cgcgtcgagc gcaccgaacg gctcatccat caaaatcagt gacggatcat
     6721 gaacgagcgc gcggcacatg gaggcgcgct gtcgcatccc gcccgagagc tcgcgcggat
     6781 aggcgttctc gaaactgccg aggcccgcgc gggcgaggag tgcgcgcgcc ttgtcgagat
     6841 agtcgcgccg cttcaggcgg cgcacgtcga tttgcaaaag cacgttgtcg agaatcgtgc
     6901 gccattccag aagcgcatcg cgctgaaaga cgatgccgac atccgtgtaa ggcgtcgcga
     6961 cgcgtgtgcc gccgatcctg atctcgccgc cggtgtaagg gacgaggccc gacaccatca
     7021 tcatcaatgt gctcttgccg cagccggagg ggccgacgat ggagacgaat tcgcccggca
     7081 tgacgtcgag cgacacgcga tcaaccgcga gtgtcgagcg gctttggtcg ggataggctt
     7141 tcaccacatc ctgcacgcgg atgagcgggt cggccgcggc aagggaagtg gtgtgttccg
     7201 ctacaaaaga cactttgtac gccttatgaa ctagcacaaa cgtcagacat taatacaaac
     7261 gctactctcc ctttcttgcc tgttcaatgt gaaaaaatag acgcatttgc actaatttag
     7321 gggcaaaacg cctaatcgat gagctttcct aaaacgcttt gtaatagtac aaatagagag
     7381 acaattttgc gaaagggacc atggcaaaca tgcgccgaaa tccacgtgcg caagcgccga
     7441 ccgagataaa tgtcgaagtg acgcgacatg tcggtggccc gttgttcgtg caattgcgtg
     7501 agcagattca ggtgctggtt gcgcgcggcg cgctgacgcc gggcgatcgg ctgccgcccg
     7561 tgcgggcgct agcggcgcag ctcaatgtca atcagatcac cgtcgcacgc gcctatagcg
     7621 aactgtccga ccttggggtg gtcgaagggc gacgcggcgg cggcacattc gtttgcgagc
     7681 gcgccgtcgt tgtggcgaaa aagcccccgg atcggaatct cgaaggggct ttccgcccgc
     7741 tgcttgccga tcgcttgttc gaattgagcc gcgcgccggg cgtcatcgct ttcacctcga
     7801 actacccttt cgtcggcacc gatatgatcg aagaatttcg cgcctgcgtg cggcttgccg
     7861 cggggcgtga tttcgaaaat tgctttcatt acgagccgcc gctcggccgc cttggcaccc
     7921 ggcaagaaat atcccgctat ctggaccgcc ggggcatgga cgtcgatccc gaaaagatcg
     7981 tcgtgacgtc gggtgcccag caggccatcg atctcgccgt gcggagcgtg gtcgaaccgg
     8041 gaacgcctgt catcgtcgag cggccagcct attacggtgc gttgaatgcg ttacgcggcg
     8101 ccggtgcaca gattctggag gcgccgctcg atggcgacgg gatcgatctg caaagcgtcg
     8161 aaacgcatct tgtcgagcat caagtccgtt tgatttacgt caatccgacg tttcagaatc
     8221 cgaccggtca gacgatgagc gaaacgaagc gccgcgatct gctggcgctc gcccgtcgtt
     8281 tcgacgcggt cattcttgaa gacgatcatt cgtccgattg gcggttcgct ggccggcctg
     8341 ttccggcgat cgccgcgctg gcagggccag aacatcgggt tttctacgct tacagcttcg
     8401 gcaagacgtt gctccccggg cagcgattcg gcttcctcgt cgtcccgccc ggcttgcgcc
     8461 gcgtcgtgct caatcgaaaa gcggcgctcg atctgcacgg cccctcgttt ttacaggaag
     8521 cgcttgcgca ctttctcctg cgcggcaatc acgaagtttt cttcgaggga gcgcggacca
     8581 gttacggcga gcgtcagaaa agcttgcgcc agcggctgtt ggcccgaatg ccgccgggaa
     8641 caaaaatcag cgcgccggac ggcggcttga gcttctggat caccttgccc gaaggggcgg
     8701 aggtgtcgga actctatttc cgcgccgtcc ggcggggcgt cgccttcgtc ccgggcgaag
     8761 cctttttcgc gacgaagccg gacatcgaag ctttgcgcgt gagtttcggg ctcgtgccgg
     8821 aaaccgagat ttccgaaggc gtcgacaggc tgtgtgctgt cgtcggcgat cttctgacgc
     8881 cgcgccccga gcgcagcgtc atcctgactt gagttcgtgc catgagggga acagacagac
     8941 tatgacgacc aagacaatta tccagccgcg cggaatggcc gaagcggttg ggcctttctc
     9001 gcgcgcgatc ctgatcggcg atcagctcca tatcgccggc acgaccgcgc tcagccatgt
     9061 cagtggcgat tattatgatc gcaaggttcc cgagacgatc gaggagcaga cgcggctgac
     9121 gctcgacaac attcgccttt gcgtcgaggc ggccggcggt acgatggccg acattttcaa
     9181 ggtcgtagtg atgttgaaga acccgtccga atataagcgc atgaacaagg tgcgcgccga
     9241 atatttcccg aaggatccgc cgatcagcac gtgctttcgc gccgaagtca tgcgtgacga
     9301 tattctggtc gagatcgaag cggtcgcgct gatcaacaag aaataatcgc aacgccgagc
     9361 aagccggcgt cgccggcttg cttcaatgcg acaggaaaac cgcctgatag gtggcaatgg
     9421 cgtcgcggat tttcgccagt tcgtcgcctg tcggattttt cgggtccttc gccggcggca
     9481 ggctcccaaa cttcatcagg caggccatga gcagcagccg ggcttttgtc gccgtcaggt
     9541 tcgaggccgc gatttgatag ggatgcgggt cggcgaagcc ttgccccccg cgcccgacgc
     9601 ggcagacggg caggccactg tataccgcct gaagcatgac tttctcgcgc gccgtcgaag
     9661 cgctgtggcc atagggcgtg agcccttcga tgacgaagcc tgtgaggcga tttaagctaa
     9721 gtttatgcgc gatcgaggcg tgcaggtccg tctccagcgc cggatcgtcg ccatagtcga
     9781 gcgtcgaata ggcgccgtcc ttgacgatcg aaacggcggg gatagcgtcg gccagcaaac
     9841 cgttctgagc atccttgacg gcgatttcga agctttcgat tgagccgtta tcggctttgc
     9901 gaacggcgcg cgtgaccgcc ggcaatcggt tgatgttcac ctcggaaaga taggtgtgct
     9961 tataggccgg cagataggtc agtgagacca cgccgaaatg gccgacgtgg ccgagaatgc
    10021 cgcctgagcc gcccgtcacg atatagccgc cgggacgcgc gtcgcccttg gcgacatcgc
    10081 gcgcggcaaa aatctgttgt tcctgaatga cgacagtgcc gcatttattg cggccttcag
    10141 cgtcagccca ggcgcgcgat gcaatgaagc gcgtcgaatc ggcgatgttc ttcggcccat
    10201 cgttgctcat ctgcccatgc gtgcgctgcg cagcgttgca gcagatcggc aaagtcgtgt
    10261 cgatgagcag attgaaccaa taggatgttt cttcgacctg cgggctgccc tgcgtccaga
    10321 tgaggccatc gtgcataccg ctattggcgg cgcgttgcac agtattggtg atcgtcgcaa
    10381 gcgccgggcg cggcgtttgt gccccaagat gccttggctg gtaggcaaag aaatccctgc
    10441 cgcggcgttc cggcgcggcg ccgcctgacg gcgcggcgcg atagaacgag acagtcgcca
    10501 ccgacgagag cggattggcc atgccgtcgc caccgaccga gaggcgatcg atctcttcga
    10561 aaagacgcga cccatcgggg aaaaatgtct ggcgggcctg ttcttttggc acatcagggg
    10621 cagcgcattc ctgctcccag ggggagccgt ccgcttggaa cgccatgtaa ggcaaggggt
    10681 aaaggccgtc ttcggggcga agttcaatct cgtagaccgg tttgtcgctc gcgttctgcc
    10741 gctcgcgatg gaagacgcca tcttcgccaa catagccgtc cggcgccgcg taaagattgg
    10801 ctgcgtcgct ttcgagcgga tgcgcggaaa attgctcgac atagatgcgc gccggcgcgg
    10861 cgaggcgctg tggacgcagc ggatcaaacg cgggctgcga actgtcggga ttgaggcgcg
    10921 ggggcagacc ctgcttttcc cgcgccttat tcgacgtgac gagcggcggc gtattctgaa
    10981 tcgtggccgt cgggccggcg atatgcgcga tgcgcacttt ggtcatgatc tgcctccgct
    11041 cggcttaagg gttcagcgca ggcggccgat ctgccgtggg ccgtgtttct cgaacaactt
    11101 gtagctgccc gagaaccagg gcagggcagc aagccgatcg cgccagggag tcttgcgtgc
    11161 ggcggcgcgc tcaggcgatt caagtgccgc tttgtcggcg agataatgca gggcgaggag
    11221 gttggctttg gtcggctcgc catcgacgac acggaagcgg cggaccatcg accagtcctt
    11281 gcagcccatg agcaacggca catggtcgag ttcgtaccag gcatcgaaat cggcgagcgc
    11341 gctttccggc acttcgaacc acacggcgaa gagcagcggc gcatcgagcg cggagacacc
    11401 actgtcgttt ggctgccgtt ttacgctggt ttcggcggca atgtagcggg tgaaaccaga
    11461 gaccgtgccg agcatttcgc gcgtcgtctc gctcggcttc gtcttcacct cttgataggc
    11521 gggggtcttc atcacggcgg gatgtgtcat ctcataaacc gcgaggtagc ccttcgcatt
    11581 ttcactaaga tcgcgataac gctgcgccga gacgaagccc ggcacatcca tgcgcagcgg
    11641 aatatgctct tcgtcatacc aattatgaaa gcgctgctcc aggccaggcg gcggcgtcat
    11701 ttcagaaaac agaaccgatg caccctgcat aatatctcct tgtcgcggcg gaacgccg
//
LOCUS       DASWOG010000672         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_329083,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000672 DASWOG010000000
VERSION     DASWOG010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329083"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGB93_13795"
     CDS             <1..738
                     /locus_tag="VGB93_13795"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0147189.1"
                     /translation="DFGFSDVVVKLSTRPDKRVGSDEAWDKAEAALQQVLDVLASRGL
                     KTEISPGEGAFYGPKLEYTLRDAIGREWQCGTTQVDFNLPGRFGAFYIGADSEKKTPV
                     MIHRASFGSLERFTGILIEHYAGHLPLWLSPVQIVVATITQDADDYAYDVVTSARKLG
                     LRAEFDLRNEKINYKVREHSLAKIPVLLVVGKREAEERTVSIRRLGTQDQVSLGLDAA
                     LAALAGEATPPDLRRLEAAKKPILVEA"
     gene            986..1729
                     /locus_tag="VGB93_13800"
     CDS             986..1729
                     /locus_tag="VGB93_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY0147190.1"
                     /translation="MPTPSAFLCFFGALLLAGCSGSVPDSEVYPTAADFEIHGIDVSR
                     YQGDIDWNSVRESGVRFAWIKATEGGDRVDEKFGVNWMAAKAAGVPRGAYHFAYWCRP
                     AEEQAAWFLQNVPNDPQALPPVLDVEWNPTSLTCPRHVPPEAARAMMKTILDAMQHAY
                     GKQPIIYTSVDFYRDVLADGAFMEYPMWVRSVKCYPDVKYAGRHWNFWQHTAQGRVPG
                     IRGYVDRNAFYGSSHDWHQWLVAEAVEGT"
     gene            1776..2579
                     /locus_tag="VGB93_13805"
     CDS             1776..2579
                     /locus_tag="VGB93_13805"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY0147191.1"
                     /translation="MTISSTPWPTLFIPHGGGPCFFMDDPQQLWTRMGAYLRGIPDAL
                     GRRPKAVLVVSGHWLESVPTVQASANPRLLFDYYNFPEHTYQLTWPAPGAPELAARVD
                     ELLRIGGFEPAAETARGWDHGVFIPFKVMWPEADVPVMQLSLQRDLDPATHLAMGRAL
                     APLRHEDVLIVGSGLSYHNLKNFFSGRGNAEAEAFDTWLDETLGAPAPARERRLVEWA
                     SAPGAVSSHPEAEHLLPLHVAVGAAADAKGQRTYHDHIVGKPISGCQFG"
     gene            complement(2662..3009)
                     /locus_tag="VGB93_13810"
     CDS             complement(2662..3009)
                     /locus_tag="VGB93_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147192.1"
                     /translation="MIVPLSYFLQTPFRNWTRMSSELIGSVMFWVDYTTPVEAVRQKL
                     SEIVENSKLWDRRVVNLQVSAVSENTMQIRALASARNSQDVWDLCCEVREKLIAFLQE
                     SYPASLPKQRAEL"
     gene            complement(3040..3132)
                     /locus_tag="VGB93_13815"
     CDS             complement(3040..3132)
                     /locus_tag="VGB93_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147193.1"
                     /translation="MQIAITQPIRIGDAVVVENEWGWIEEITGT"
BASE COUNT          643 a          993 c         1062 g          727 t
ORIGIN      
        1 gatttcgggt tcagcgatgt cgtcgtgaaa ctctcgacgc ggccggacaa gcgtgtcgga
       61 tcggatgagg cgtgggacaa ggctgaggcc gcgttgcagc aggtgctcga cgtgctggca
      121 tcgcgtggcc tgaagactga gatcagcccc ggcgagggcg cgttctacgg gccgaagctc
      181 gaatatacgt tgcgcgatgc gatcggccgc gaatggcaat gcggtacgac gcaggtcgat
      241 ttcaatctgc ccggtcgttt cggcgctttc tacatcggcg ccgatagcga gaagaaaacg
      301 ccggtgatga tccatcgcgc cagtttcggc tcgttggagc ggtttaccgg cattttgatc
      361 gagcattacg ccggccatct gccgctctgg ctctcgccgg tgcagatcgt ggtcgcgacc
      421 atcacgcagg acgcagatga ttacgcctat gatgtcgtga cgtcagcgcg caaattgggc
      481 ctgcgcgctg aattcgacct gcgcaacgag aagatcaact acaaggtgcg ggagcattcg
      541 ctggccaaaa tcccggtcct gcttgtcgtt ggcaagcgcg aggcggagga gcgcacggtc
      601 tccatccgcc gtctcggcac gcaggatcag gtgagcctcg ggctcgacgc ggcgctggct
      661 gcgctcgccg gcgaggcgac gccgccggat ttgcgccgac ttgaggcggc gaagaagccg
      721 atacttgtcg aggcttaagc tacgcttgag cctcgatggt gaattatctg atttttaacc
      781 gtggctttgc cttttcattg ccgtaagctc aaggcataaa agtaatgcgg tagaggggtt
      841 tgcgcgttgc cacgcgtgcg agaggccatc gcgcattaac gggtcgttac ccggcatcgg
      901 ataaatcaat cgaatgatcc gacgtcttgc tgtgccgaaa ggtggaattc cggcgtccag
      961 ccggtttggg gtgggcagtt ttggcttgcc gaccccgagc gcctttcttt gcttttttgg
     1021 tgcgcttctg ctcgccggtt gttcgggctc agtcccggac agcgaagtct atccgaccgc
     1081 cgccgatttc gagatccatg gcatcgacgt ctcccgctat cagggcgata tcgactggaa
     1141 ttccgtgcgc gaatccggcg tgcgcttcgc ctggatcaag gcgacggaag gcggcgaccg
     1201 cgtcgatgaa aaattcggtg tgaattggat ggcagcgaaa gcggccggtg tgccgcgggg
     1261 ggcctatcat ttcgcctatt ggtgccggcc ggctgaggaa caggccgcct ggttcctgca
     1321 aaatgtgccg aacgatccgc aggcgcttcc gccggtgctc gatgtcgaat ggaacccgac
     1381 gtctctgacg tgtccgcgcc acgtcccgcc cgaagccgcg cgggccatga tgaagaccat
     1441 tctcgacgca atgcagcacg cctatggcaa gcagccgatc atctacacgt cggtcgattt
     1501 ttatcgcgat gttctcgccg atggcgcgtt catggaatat ccgatgtggg tgcgcagcgt
     1561 caaatgctat ccagacgtca agtatgccgg acggcattgg aatttctggc agcacacggc
     1621 gcaggggcgt gtgccgggca ttcgcggtta cgtcgaccgc aatgcctttt acggctcatc
     1681 gcatgactgg catcaatggc ttgtcgccga ggcagtcgaa ggcacttaat cgctgcaagc
     1741 cttgcgaagc cgccgctcgc gcccatatcg cgtcaatgac aatctcatca acgccctggc
     1801 cgacactatt cattccccac ggtggcggtc cgtgtttctt catggatgat ccgcagcaac
     1861 tctggacgag aatgggcgct tatttgcgcg gtatccctga cgcccttggg cgccgcccga
     1921 aagccgttct tgtcgtctcc ggccattggc tcgaatctgt gcccacagta caggccagtg
     1981 ccaatccgag gcttctcttt gattattata attttcccga acacacctat cagttgacgt
     2041 ggcccgcgcc gggtgcgcca gagcttgcag cacgtgtcga cgagcttttg cgcatcggcg
     2101 gttttgaacc cgctgcggaa accgcacgtg gttgggacca tggcgtcttc ataccgttca
     2161 aggtgatgtg gccggaggct gatgtgccgg tcatgcagct ttccttacaa agggatctcg
     2221 atccggcgac acatcttgcg atgggccgtg cccttgcccc gctgcggcat gaggatgtgc
     2281 tcatcgttgg cagcggctta agctatcaca atctgaagaa tttcttttcg ggccgcggta
     2341 atgccgaggc cgaagcattt gatacctggc tcgacgaaac attgggcgcg ccggcccctg
     2401 cacgggagcg tcggctggtc gaatgggcaa gcgcgccagg cgccgtctca agccatccgg
     2461 aggcagagca tcttttgccg ctgcatgtcg ccgtcggcgc tgctgccgat gcgaaggggc
     2521 aacgcactta tcatgaccat attgttggca agccgatttc aggctgtcaa ttcggctgat
     2581 tgggcgatgg cttcttggcc gttcgcagcc gcgcgcggac caggcggcgg atttgcgggg
     2641 ccgtgggcgg ccatcgagcc cttacaactc ggcacgctgt ttgggcaggg aggcaggata
     2701 gctttcttgc aaaaatgcga tgagcttttc gcgcacttcg cagcacaggt cccagacgtc
     2761 ctgcgaattc cgggcgctcg ccaaggcgcg aatctgcatg gtgttttcgg aaacagccga
     2821 gacttgcaga ttgacgacgc gccgatccca taattttgaa ttctcgacga tctcgctgag
     2881 cttctggcgc accgcttcga cgggtgtggt gtagtccacc cagaacatga cgctgccgat
     2941 gagttcggac gacatgcgcg tccagttccg gaagggcgtt tgcagaaaat acgaaagcgg
     3001 cacgatcatt cgccgccaat cccagatctg gatgacgact taagtgccgg taatttcctc
     3061 gatccatccc cattcgttct cgacaacgac ggcatcgccg atgcggatgg gctgcgtgat
     3121 cgcaatctgc acaccggcaa tgagatggcg agaacggggc gcgcggcgag gccgacgatg
     3181 atacctgccg cgccggccga cgcgatcagg ctgacgccga attgacgcac ttgcgggatg
     3241 ctcattaggg cgaccgcaat gatgatcatc ataaccaccg tttccgccgc gcggcgcagc
     3301 acgcggacct gcgtcacatg tttgcgcgcc agcagattgt cggcgaggtc gatcttgaac
     3361 cggccgatat agaaatcccc ggcaagctcg atcgccatca tggatgccca gccgattagc
     3421 aatgt
//
LOCUS       DASWOG010000673         4859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_162998,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000673 DASWOG010000000
VERSION     DASWOG010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162998"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VGB93_13820"
     CDS             <1..922
                     /locus_tag="VGB93_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147194.1"
                     /translation="WAYGAPGDHAEMVALIRHAVEIGVTFFDTAEVYGPRTNEELVGE
                     ALAPLRDRVKIATKFGFMLDPQSGRPVGLNSKPAHIKEVADASLKRLRTDHIDLFYQH
                     RVDPNVPIEDVAGAVADLVKAGKVRHFGLSEAGAATIRRAHAVHPVAAVQSEYSLWTR
                     GPEKDVLPTLEELGIGFVPYSPLGRGFLAGTFSADTQFADDDWRKTNPRFQADALRKN
                     APLVDSLKAIADEKGATPAQIALAWLLAQKPWIVPIPGTTKRTRLEENIKAADITLTP
                     NDLKAIEEAVASHAVTGERYDERGMAMVNL"
     gene            946..1371
                     /locus_tag="VGB93_13825"
     CDS             946..1371
                     /locus_tag="VGB93_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY0147195.1"
                     /translation="MMALYSTKVVTSGGRHGMIRSEDGLLDLRLALPKSLGGRGDATN
                     PEQLFGGGYGACFENALFHVARDAGHKFADDDIEVVAEISLDKNAEGSFVLSAALAVT
                     IAGVDLETAQKLVDGAHHICPYSNAIRGNVDVKTSVSIR"
     gene            complement(1403..2737)
                     /locus_tag="VGB93_13830"
     CDS             complement(1403..2737)
                     /locus_tag="VGB93_13830"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY0147196.1"
                     /translation="MNEQVKAPRTTPRFSSTFDAYTLSEIRRAAATGIYDIRGAGAKR
                     HLPHFDDLLFLGASMSRYPLEGYREKCGTAVTLGTRFAKKPIELKIPITIAGMSFGSL
                     SAQAKEALGRGASTVGTSTTTGDGGMTQEERGHSKILVYQYLPSRYGMNPDDLRKADA
                     IEVVVGQGAKPGGGGMLLGQKISPRVAQMRTLPEGIDQRSACRHPDWTGPDDLEIKIE
                     ELREITDWEKPIYVKVGASRPYYDIALAVKAGADVIVLDGMQGGTAATQEVFIEHVGI
                     PILAAIRPAVKALQDLGMHRKVQLIVSGGIRNGADVAKALALGADAVSIGTAALVALG
                     DNDPHWQADYEALGSAAGAYDDWHEGRDPAGITTQDPNLAKRLDPTAAGRRLANYLSV
                     LTLEAQTIARAAGKSHVHNLEPEDLVALTVEAAAMAGVPLAGTDWIPGTGGR"
     gene            complement(2737..3483)
                     /locus_tag="VGB93_13835"
     CDS             complement(2737..3483)
                     /locus_tag="VGB93_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HEY0147197.1"
                     /translation="MNIQSQTVKKAAGKTDLGHARTFDLATNSVRELNRALHAIHPGS
                     NANLFRVLNPRGVHAIAAGVDADVTIEIDGDVGYYCGGMNQLATILIEGSAGIGLGEN
                     MMSGLIHVRGNASDSVGATAHGGSIIIDGDASGRCGISMKGVDIIVKGSIGPMSAFMA
                     QAGTLAVFGDVGEAFGDSLYEAKLYARGNVASLGADCIEKEMDDQHRAELYEKFKELG
                     LAGEVDVGEFKRYGSGRTLYHFHVDNAGAY"
     gene            complement(3480..4370)
                     /locus_tag="VGB93_13840"
     CDS             complement(3480..4370)
                     /locus_tag="VGB93_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HEY0147198.1"
                     /translation="MCGIVGLFFKDPALEPDLGKHLSVMLETMTARGPDSAGFAVYGD
                     GEKSRTKITLRAPADFEGYAALEKALESGASFTRRGTHIIISVPTVDVEAVLQKIKHA
                     VPTLTVSSIGTHMELYKEVGLPADVAKKFHLAEMSGTHGIGHTRMATESAVTTAGAHP
                     FSTGKDQCLVHNGSLSNHNQMRRKLIREGIPFATENDTEVAAGYVSWRLAQGDSLGEA
                     LESSLKDLDGFYTFVVGTDTGFGVLRDPIGCKHAVMAETDQYVAFGTEFRALSSLPGI
                     ENAKIFEPNPATVYFWNRAA"
     gene            complement(4574..>4859)
                     /locus_tag="VGB93_13845"
     CDS             complement(4574..>4859)
                     /locus_tag="VGB93_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0147199.1"
                     /translation="MLSAGLRLVHTGEIALPRLLAAMLSRPAEILGLPQGRLEIGAPA
                     DLIRFDPDEPYVLDPATLHSRCKNTPFDEARMQGRVKLTIVAGELVWREG"
BASE COUNT          945 a         1491 c         1485 g          938 t
ORIGIN      
        1 ctgggcctat ggcgcgcccg gcgaccatgc cgagatggtc gccctcattc gtcatgccgt
       61 cgaaatcggc gtcaccttct tcgatacggc ggaggtttat ggcccgcgta ccaacgagga
      121 attggtcggc gaggctttgg cgccgctgcg cgatagagtg aagatcgcga cgaaattcgg
      181 tttcatgctc gatccgcaaa gcggtcgccc cgttgggctt aacagcaagc cggcgcatat
      241 caaggaagtt gcggatgcgt cgctgaagcg gctacgcacc gaccacatcg atctgttcta
      301 ccagcatcgc gtcgatccca acgtgccgat cgaggatgtc gcgggtgcgg ttgccgatct
      361 cgtcaaggct ggcaaggtga ggcacttcgg cctttcggaa gctggcgcgg cgacgatccg
      421 gcgggcgcat gcggtgcatc ccgtcgcggc ggtgcagagc gaatattcgc tgtggacgcg
      481 ggggccggaa aaagatgtgt tgccgacgct cgaagagctc ggcatcggct ttgtgcctta
      541 cagtcccctc ggccgtggct tcctcgccgg tacattttcc gcagacacgc agtttgccga
      601 tgacgattgg cgcaagacca atccgcgctt ccaggccgac gcgctgcgca agaatgcgcc
      661 gcttgtcgat agtctcaaag ccattgccga cgagaaaggc gcaacgccgg cgcagatcgc
      721 gctggcctgg ttgcttgcgc aaaagccctg gatcgtgccg atccccggca cgaccaagcg
      781 cacgcggctc gaagaaaaca tcaaggccgc cgacatcacg ctgacgccga atgatctcaa
      841 ggcgatcgaa gaggctgtcg catcacatgc cgtcacgggc gagcgctacg acgagcgcgg
      901 tatggcgatg gtcaatctct aacgctataa atttcaagga gacgagtgat ggctctatat
      961 tcaacgaaag tggtcacgag cggtggccgc cacggtatga tccgcagcga agacggcctg
     1021 ctcgacctga ggctcgcgtt gcccaaatcg cttggcggcc gcggcgacgc gaccaacccg
     1081 gagcagctct ttggcggcgg ctacggcgcg tgttttgaaa atgcactgtt tcatgtcgcg
     1141 cgcgatgcgg gccataaatt tgcagacgac gacatcgagg tcgtcgctga gatcagcctc
     1201 gacaaaaacg cggaaggcag cttcgtgcta tcggccgccc tcgcggtgac gatcgccggt
     1261 gtcgatctcg aaaccgcgca gaagcttgtc gatggcgcac accacatctg cccctattcc
     1321 aatgcgataa ggggcaatgt tgacgtgaag acgagcgtgt ccattcgctg aatcctcccc
     1381 gctccaaggc gagggaaaat cctcagcgcc cgccagttcc cggaatccaa tcggtgccag
     1441 ccagcggcac gccggccatg gcggctgcct cgaccgtcag cgccacgagg tcttccggtt
     1501 caagattgtg gacgtggctc ttgccggcgg cgcgggcaat cgtctgcgct tccagcgtca
     1561 gcactgaaag gtaattggcg agcctacgcc cggccgcggt cggatcgagc cgtttcgcca
     1621 gattcggatc ctgcgtggtg atgcccgccg ggtcgcggcc ctcgtgccaa tcgtcataag
     1681 cgccggcggc gcttcccagc gcctcataat cggcctgcca atgcggatcg ttatcgccaa
     1741 gcgcgacaag agccgcggtg ccgattgaga ccgcatcggc gcccagcgcc agggccttgg
     1801 cgacatcggc accgttgcgg atgccgccgg agacgatgag ctgcactttg cggtgcatcc
     1861 ccaggtcctg cagcgccttc actgccggac ggatcgccgc gaggatcgga atgccgacat
     1921 gttcaatgaa gacttcctgc gtcgctgccg tgccgccctg catgccatcg agcacgatca
     1981 catcggcgcc cgctttcacc gccagcgcga tgtcgtaata aggtcgcgag gcgccgactt
     2041 tcacgtagat cggcttttcc caatcggtga tctcgcgcaa ttcctcgatc ttgatttcga
     2101 gatcgtcggg tccggtccaa tctgggtggc ggcaagccga gcgctggtcg ataccttcgg
     2161 gcagcgtgcg catctgcgcg acgcgcggcg agattttctg gccgagcagc atgccgccgc
     2221 caccgggctt agcgccctgc ccgacgacga cttcaatcgc gtcggccttg cggagatcgt
     2281 ccggattcat gccgtagcgc gacggcagat attggtagac gagaatcttg gaatggccgc
     2341 gctcttcctg cgtcatgccg ccgtcgccgg tcgtcgtcga tgtgccgacc gtcgaggcac
     2401 cgcgacccag cgcttctttc gcctgcgccg acagcgagcc gaagctcatg ccggcaatgg
     2461 tgatcgggat tttgagttcg atcggctttt tggcgaaccg cgtgccgagc gtgaccgccg
     2521 tgccgcattt ctcgcgatag ccttcgagcg gatagcgcga catcgaggcg ccgagaaaca
     2581 gcaaatcgtc gaaatgcggc agatgccgct tcgcgcccgc gccacgaata tcatagatgc
     2641 ctgtcgcggc ggcgcgacga atctcggaaa gtgtgtaagc gtcaaatgtc gatgaaaaac
     2701 gcggcgtcgt acgtggagct ttaacctgct cgttcatcaa taggcccctg cattgtcgac
     2761 gtggaaatga taaagcgtgc gccccgagcc atagcgtttg aattcgccga catcgacctc
     2821 gcccgcgagc ccaagctctt tgaacttttc gtagagttcg gcgcgatgct gatcgtccat
     2881 ctccttttca atgcaatcgg cgccaaggct cgcgacgttc ccgcgcgcat aaagcttggc
     2941 ctcatagagc gagtcgccga aggcttcgcc gacatcgccg aagacggcga gcgtgcccgc
     3001 ttgcgccatg aaggctgaca tggggccgat ggagcccttc acgataatat cgacgccttt
     3061 catcgaaatg ccgcagcggc ccgaagcatc gccatcgata atgatcgaac cgccatgcgc
     3121 cgtcgcgccg acggaatcgc tggcattgcc gcgaacgtgg atcaggcccg acatcatatt
     3181 ttcgccaagg ccgatgccgg cgctgccttc gatgaggatc gtcgccagct ggttcatgcc
     3241 gccgcaataa tagccgacgt caccatcgat ctcgatcgtt acgtccgcat cgacacccgc
     3301 tgcgatggca tgaacaccac gcggattgag cacgcgaaat aaattcgcat tgctacccgg
     3361 atggatcgcg tgcagcgcac ggttcaactc gcgcacagaa ttcgtcgcga gatcaaacgt
     3421 gcgggcatgg cccagatcgg tcttgcccgc agctttcttc acggtttgcg attggatgtt
     3481 catgccgcgc ggttccaaaa atagacggtg gcgggattcg gctcgaaaat ctttgcgttc
     3541 tcaatgccag gcagtgaact cagagcgcga aactcggtgc cgaaggcaac atattgatcg
     3601 gtctcagcca taacggcgtg tttgcagccg atcggatcgc gcagaacgcc gaagccagtg
     3661 tcagtgccga cgacgaaagt gtaaaagccg tcgagatcct tcagcgagga ttcgagcgct
     3721 tcgccgagac tgtcaccctg cgccaagcgc caggaaacat agccggcggc cacttccgtg
     3781 tcgttttcgg tcgcgaaggg aatgccctcg cggatcagct tgcggcgcat ctgattatgg
     3841 ttcgacagcg agccattgtg gacgaggcat tgatccttgc cggtggagaa cggatgcgcg
     3901 cccgccgtcg tcaccgcaga ttccgtcgcc atgcgggtat ggccgatgcc atgcgtgccg
     3961 ctcatttcgg cgaggtggaa tttcttggcg acatcggccg gcaggccgac ctctttataa
     4021 agctccatat gcgtgccgat ggagctgacg gtcagcgtcg gcacggcgtg cttgatcttc
     4081 tgcaggacgg cttcgacatc aaccgtcggt accgagatga tgatgtgggt gccgcggcgc
     4141 gtgaagctcg cgccagactc cagcgctttt tccaaggctg cgtagccttc aaaatccgcc
     4201 ggcgcgcgca gagtgatctt ggtgcggctt ttctcgccat cgccatagac ggcgaaaccg
     4261 gcgctatcgg ggccgcgtgc cgtcattgtc tccagcatga ccgagagatg cttcccgaga
     4321 tccggttcaa gagctggatc tttgaagaag agtccaacga tgccgcacat cccactgcct
     4381 cgcgtgtttc ttaaacctaa agcaattttc ctataaagaa aatagcagtc gcgtgaagaa
     4441 actgaaaggg ggattttgcg cttttcgtca aaaaaattac gtttgcgcta cggcaaagcc
     4501 ggtcttgcat aaatccggcc aagcgtgggc ctaggggcgc gaggctctac ggagggcaat
     4561 gcctgaggcc gtctcagcct tcgcgccaga cgagttcgcc cgcgacgatg gtcagcttca
     4621 cccgcccctg catccgggct tcgtcgaagg gcgtgttctt gcagcgcgaa tgcagtgtcg
     4681 ccggatcgag cacatagggc tcatcgggat cgaaacggat gaggtcggcc ggcgcgccga
     4741 tctcgagccg cccctgcggc aggccgagaa tttccgccgg ccgcgacaac attgccgcga
     4801 gcagtcgtgg cagcgcgatt tcgcccgtgt gcacgagccg caacccggcc gacagcatt
//
LOCUS       DASWOG010000674         5892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_35484,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000674 DASWOG010000000
VERSION     DASWOG010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35484"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VGB93_13850"
     CDS             <1..288
                     /locus_tag="VGB93_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEY0147200.1"
                     /translation="TEWGGIARDSLIARSGKTPYGPFAQRMAKIFDSDAAARRASPPE
                     AVANVIGDAVAAKRPHIRYAAAGGAPMFLFLARVLPDRAKDAIISRMTQGN"
     gene            726..1064
                     /locus_tag="VGB93_13855"
     CDS             726..1064
                     /locus_tag="VGB93_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147201.1"
                     /translation="MNPALAYLVLAIAAALEAGGDAIVRKGLHASGTSTRIGIFALGA
                     AILFAYGVTVNSPPWDFGKLLGIYVTLFFVVAQIVNLIFFDARPDLPIFVGGALIVAG
                     GLVITFWRAS"
     gene            1136..2002
                     /locus_tag="VGB93_13860"
     CDS             1136..2002
                     /locus_tag="VGB93_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147202.1"
                     /translation="MTTYSVHYRTIEVDGLSIFYRETGPQDAPTLLLLHGLPSSSRMF
                     EPLLQRLADRYHLVAPDYPGFGHSDWPTPEHFAYTFDHIAEVIDGFTQKLSLSRYSLY
                     MQDYGGPVGFRLAQAHPERIETLIIQNAVAHDTGLGPNWITRRAFWVDRATHEAALKT
                     NLLSLETTRARHVGSDPNVDRYDPDLWTDEFAFLSAPAQAEIQTDLFYDYRTTVASYP
                     TWQAWMRERQPKMLVLWGKYDSSFQISEPDAYKHDVPTAEIHILDGGHFALDTAAEKA
                     AELIDGFLTKTR"
     gene            2140..2397
                     /locus_tag="VGB93_13865"
     CDS             2140..2397
                     /locus_tag="VGB93_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147203.1"
                     /translation="MRKNFQKFVGVTFLAAAVLAPIAAYASHHHHFVSVEMMRHTMAT
                     MKDGTKMHVQVVKMNGHIMVMIPMADLPDYLRQQIFVPGDQ"
     gene            2503..3555
                     /locus_tag="VGB93_13870"
     CDS             2503..3555
                     /locus_tag="VGB93_13870"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0147204.1"
                     /translation="MPSSSQADLSQKLGLKYPLIVAPMAGGPSSVDLVAASSTAGALG
                     SMGAAYSNPAAIGQFADAVRQRTDRPFAINLFIPAPVPQINRDQVDRAIEATSQYRLE
                     LGLSTPDLAPPFEEDFDAQFEAVLKAKPAVLSFVFGVLRAERSRAARDAGILLIGAAT
                     TFVEAQTLDDSSVDAITLQGFEAGGHRGIFDASARDEEIPLHDLLTQCVGCIRSPLIA
                     AGGIMAAPGVDAALRAGAQGVQLGTAFLACAETGTSKPYRGRLLDPAPRVTRTTRAFS
                     GRLARGIENRFMKEMAAKADVILPFQAQNKFTRDIRNASAARGSADFLSLWAGTGEGE
                     LWQGPVAELIERLFQR"
     gene            3685..3960
                     /locus_tag="VGB93_13875"
     CDS             3685..3960
                     /locus_tag="VGB93_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147205.1"
                     /translation="MSIARDDLESTVHFFENALHALENAQTRGRVIQIKFKADAMAML
                     AKRLSDSALHAEANMVSRITMERLLDFPEDETRPVANGSGNRVSVFC"
     gene            complement(4320..4409)
                     /locus_tag="VGB93_13880"
     tRNA            complement(4320..4409)
                     /locus_tag="VGB93_13880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4373..4375),aa:Ser,seq:tga)
     gene            4881..5606
                     /locus_tag="VGB93_13885"
     CDS             4881..5606
                     /locus_tag="VGB93_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY0147206.1"
                     /translation="MLRKLLTTTALICLAGGTALAADLPSEKGPPVYAPPPPPAFSWS
                     GVYLGGQVGYGWGTTHFTDPAGPFPGLSQSGVIGGVHIGYNYQVSQFVFGLEGDVNGS
                     SERDTGVTADGILNYSLRENIDASIRGRVGYAFDRVLIYATGGGAYGSFHTGYNDGVT
                     FDGFNHGRIGWTVGGGIEYAIDNNWSVRAEYRYTDYGHFTDFPASDAGGPVNFHIRDN
                     RAQVGFSYKFDMFSPLAPIVSKY"
     gene            5736..>5892
                     /locus_tag="VGB93_13890"
     CDS             5736..>5892
                     /locus_tag="VGB93_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0147207.1"
                     /translation="MREPSTATVAASPPKISFVSLGCPKALVDSERIITRLRAEGYEL
                     TRSHAGAD"
BASE COUNT         1167 a         1744 c         1646 g         1335 t
ORIGIN      
        1 accgaatggg gcggcattgc ccgcgacagt ctcatcgctc gttccggcaa aacaccctac
       61 ggtcccttcg cgcagcgcat ggccaaaatt ttcgatagcg atgcggcggc gcgccgggcc
      121 tcgccgccag aggccgtggc aaatgtcatc ggagacgccg tcgcggcaaa acggccgcat
      181 atccggtacg cggcggcggg cggcgcgcca atgtttttat tcttggcacg cgtcctaccc
      241 gatcgagcga aggacgcaat catcagtcgc atgacacaag gaaattagtt atcaggctct
      301 ctcgctattg cgacccgccc gcgccgtagc ctccaccgct gccgaaaccg ctattgctac
      361 cgcgatgatg gcggctggtg ctggagcttg aatcgcttgc gggtgtatcg gacggtgtct
      421 ggtttgagca tgtccaccca gttgcgctgc gcgacgagcg tactgcccag gttcccgccc
      481 cgcagagcgg cggcgccgac tgcgcatagg cgcgttcatg ggcgttttgg aataagccgc
      541 ccaaagccag accgaagctc acaacaacga gcaatttacg catttgatcc ctcccggatt
      601 tacgtgcatt gatttgctgc accagagaag ctttggctga ttgtgcggct attcagggcc
      661 acgattcagg caggatttcg ctcagcgcat ccctaactga aatttgaata agccgacggt
      721 gtccgatgaa tccagccctt gcctaccttg tcctggcaat tgccgcagct cttgaagcag
      781 gcggtgacgc tatcgtgcgc aagggcctcc acgcttcggg tacatcgacg cggatcggaa
      841 tcttcgcatt gggcgccgca attctcttcg cttatggcgt gacggtgaat tcaccgccgt
      901 gggatttcgg caagctgctt ggtatctatg tcacgctgtt ctttgtcgtc gcacagatcg
      961 tcaatctcat ctttttcgac gcacgccccg acctgccgat ctttgtcggc ggagcgctga
     1021 ttgttgccgg cggccttgtc atcacctttt ggcgtgcgag ttaggccgcg ctttcgacga
     1081 tctctgggat gttgtaggtc tctgcccacg cgatccgcaa aaatgggctg tggctatgac
     1141 gacatactca gttcactacc gcacaattga ggtcgatggg ctttccatct tctatcgcga
     1201 aaccggcccg caggatgcac cgacgctttt gctcctgcac ggattgccgt cttcgtcgcg
     1261 gatgttcgag cctcttttgc aacgtctggc agatcgctac cacctcgtgg cgccggacta
     1321 tccgggcttc ggccatagcg actggcccac gcccgagcat tttgcctaca cgttcgatca
     1381 tattgcggaa gtcatcgatg gctttacgca gaagctcagt ctgtcgcgct acagcctcta
     1441 catgcaggat tacggaggcc ccgtcggctt tcggcttgcg caagcccacc ccgaacgaat
     1501 cgaaacgctc atcattcaga acgctgtcgc gcatgacacg gggctcggcc ctaattggat
     1561 cacgcggcgc gccttctggg tggatcgcgc gacgcacgaa gcggcgctga aaacaaatct
     1621 tctgtcgctt gagacaacgc gggcgcgtca tgtcggcagc gatccgaacg tcgatcgcta
     1681 cgatccggat ttgtggacag acgagttcgc ttttttgagc gcgcccgccc aagccgaaat
     1741 tcagacggac ctgttctacg attatcgcac cactgtcgca tcctatccaa cttggcaggc
     1801 ctggatgcgc gagcgtcagc cgaagatgct ggtgctgtgg ggaaaatacg attcgtcttt
     1861 ccagatatcc gagccggatg cttataaaca cgacgttcct acggccgaga ttcacattct
     1921 cgatggaggt cattttgcgc tcgacacggc ggccgaaaaa gctgctgaat tgatcgacgg
     1981 ctttctgacg aaaactcgct gataaagcta tagagcgtct aaatacccgc taaatttata
     2041 gactttgaag aatatccaaa atatttagat cgaatacttt gttcatgcta agtaaaccgc
     2101 gcgaagaggc gccgtgcccc gcgttatagg agtctgctta tgcgcaagaa tttccagaag
     2161 ttcgttggcg ttacatttct cgccgccgcc gtgctcgccc ccatcgccgc atacgcaagc
     2221 caccatcatc actttgtttc ggtcgagatg atgcgccaca cgatggccac gatgaaagac
     2281 ggcacaaaga tgcatgttca agtcgttaaa atgaacggcc acataatggt tatgattccg
     2341 atggccgatc ttcccgatta tctgcgccag cagattttcg ttcctggcga tcaataagca
     2401 attcctttga cgccaatctg ataaagtcag atcgatacgc acggatgtat cgcgtatcga
     2461 tcctccggcc gttaaccaat gtgatcggac ggacattcgg acatgccatc aagctcgcaa
     2521 gccgatcttt cgcaaaagct cggtttaaaa tatccgctga tcgttgcgcc tatggcgggt
     2581 ggcccgtcgt cggttgacct ggtggcggca agttccaccg cgggcgcatt gggctccatg
     2641 ggtgccgctt actcgaatcc ggcggcgatc gggcaatttg ccgacgcggt acggcagcgg
     2701 actgaccgac cctttgcgat caatctcttc attccagcgc ccgttccaca gatcaaccgc
     2761 gaccaagtcg atcgcgctat cgaagctacg tcgcaatacc ggctcgaact cggtctttcg
     2821 actcccgatc tggccccgcc ctttgaagag gatttcgacg cccaattcga agctgtccta
     2881 aaagccaaac ccgccgtttt gagctttgtc ttcggcgtac tgcgtgccga gcgtagcaga
     2941 gccgcgcggg atgctggcat tcttctcatc ggcgccgcga caacgttcgt ggaagcgcaa
     3001 acgctcgacg acagcagtgt cgatgccatc accttgcaag gattcgaagc cggcggtcat
     3061 cgtggaatct ttgacgctag cgcgcgggat gaggaaatcc cccttcacga cctgctgacg
     3121 caatgcgtcg gttgcatccg gtccccactc atcgccgctg gcggcatcat ggccgcgcct
     3181 ggtgtcgatg cggcgcttcg cgccggtgcg caaggggtac agttgggcac cgcgtttttg
     3241 gcctgcgccg agaccgggac gtccaagccc tacaggggga gacttctcga ccctgcaccg
     3301 cgagtgacca gaacaacccg tgccttctcc ggccgcctgg cgcgcggtat cgagaatcgc
     3361 ttcatgaagg agatggcagc gaaggccgac gtaattcttc ccttccaggc gcaaaacaaa
     3421 ttcacccgcg atatccggaa cgcctcggcg gcacgcggct ccgcggactt cctgtcgcta
     3481 tgggccggca cgggcgaagg cgaactctgg caaggccccg tggcggaact catcgagcgg
     3541 ctgttccaaa ggtgatcggc atctggcagc ggccgccgct cggcgcgtgt gctcggtgag
     3601 ggctcccaaa cggcaagttg ttaaaatatt aattcaaatg ccgcataacg gcgacgttat
     3661 ccgagctacg ccccagaggc tgtcatgagt attgcgcgcg acgatctaga gtcgaccgtc
     3721 cactttttcg agaatgctct ccacgcgctc gagaatgctc agacccgcgg gcgtgtgatt
     3781 cagatcaagt ttaaagcgga cgcgatggcc atgttggcga agcggctttc cgactccgcg
     3841 cttcacgcag aagcgaacat ggtctcgcgc atcaccatgg aacgattatt ggattttcca
     3901 gaggatgaaa cgcgacccgt tgcaaacggc tcggggaacc gcgtttccgt tttctgctaa
     3961 cggaccggcg aagacgctag cggaaatccg cgatccatcg catagctgga tttgcacttc
     4021 gccgtttttc gtagcgaagc cgtcgagcgt cacgacgtga ccggggtttt ccgcagccct
     4081 gctctgcgtt ttatgaagct ctcgttccgc caatccgggc gagtccgccc cgatcacgaa
     4141 tcgttcgacg tgattagcaa aacgtgttgc aaaaataccg gaacgaaaac gcggccaaaa
     4201 agcttctccg agcggtccgg caatcgtttc gcttaagcgc gattcgatcc gggtttaaga
     4261 gccgagaagc tttttcgctc gtcctaaaga cgttccattt cagcgacttg aagagatgct
     4321 ggcggaaggg gtgggattcg aacccacggt gggctttcac ccacggcggt tttcaagacc
     4381 gctgccttaa accgctcggc cacccttcca tgttgtgcgt tatggcacgg cgacggccga
     4441 acgctaaagc gagattttag ccgtggttgg tttcaaaatt tgggggctta aggaaacttt
     4501 gaccagcgcc atgccaattg ggggcgtcag ggcaaattgg cggcatcgat cgcggtaaat
     4561 gttcttccgc aataaatgct tcagctttgc agctttgagt tcgattaacg gaaggtttag
     4621 accgcgcgca ttgcgcgtgt tttcaagttc cggtgattaa actttgcttc ttcggcttgc
     4681 cacatttgcg ccctcaattt tgcttgttgg acgctctagc aagccagcgc tcgaagtttg
     4741 tctttttgca gcgttgcata agtactacat cgcggcgcta aacaaggttt ggacttggtt
     4801 ttcggtctca cgtctattga tccagacaac ggtaggattt agctggctgc atgatgccaa
     4861 ctcgaagagg ggatttccta atgttgcgta agcttctaac aacgacggcc ctgatctgcc
     4921 ttgccggcgg cacggcgctc gcggccgatc ttccgagcga aaagggtccg cccgtttatg
     4981 ctcccccgcc gccgccggct ttcagctggt cgggcgtcta tctcggtggc caggtcggct
     5041 acggctgggg caccactcac ttcactgatc ccgctggccc atttcctggc ttgtcacaga
     5101 gcggcgtcat tggcggtgtt catatcggct ataattacca ggtttcgcag tttgtcttcg
     5161 gcctcgaggg cgatgtgaac ggctcgtccg agcgggatac cggtgtcacc gccgacggca
     5221 tacttaatta ttcactccgc gagaacattg atgcttcgat ccgtggccgc gttggctacg
     5281 cgttcgaccg tgtcttgatc tacgctacag gcggtggcgc ctatggtagc ttccataccg
     5341 gctataacga cggcgtgact ttcgacggct tcaatcatgg ccgtatcggc tggaccgtcg
     5401 gcggcggcat cgaatatgcc atcgacaaca attggtcggt ccgtgccgaa tatcgttaca
     5461 cggactatgg tcatttcacc gacttccccg cttcggatgc cggtggcccc gtcaatttcc
     5521 acatccgcga caaccgcgct caagtgggct tctcgtacaa gttcgacatg ttctcgccgc
     5581 tggcgccgat cgtttcgaag tactgaggac ggctttagga aatccaagaa cccggcccct
     5641 gtggccgggt tttttcattg tggcgcctcc ccggccctac agttccgatt cgcgcccttg
     5701 cggcggcggc cgagacatgg cactgcacgg ccatgatgcg cgagccatcg acggcgacag
     5761 tggccgccag ccctcccaaa atctccttcg tttctctcgg ctgtcccaaa gctttggtcg
     5821 acagcgagcg aatcatcacg cgactgcgcg ccgagggtta tgagctgacc cgcagccatg
     5881 ccggcgccga tg
//
LOCUS       DASWOG010000675         8681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_52846,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000675 DASWOG010000000
VERSION     DASWOG010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52846"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2858)
                     /locus_tag="VGB93_13895"
     CDS             complement(<1..2858)
                     /locus_tag="VGB93_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEY0147208.1"
                     /translation="MSGPLRLPNGGLVDRRREIAFKFDGRHYLGHPGDTLASALMANG
                     VKLVGRSFKYHRPRGIFTAGPEEPNALVTLRDGARREPNTKATTIELYDRLVATSQNC
                     WPTLSLDILSINQLFAPLFVGGFYYKTFMWPASFWEKVYEPAIRRAAGLGAASGLPDP
                     DYYEHATAYCDVLVIGSGPAGLAAALAAGRAGARVIICEEDFRLGGRLLSEAREIDSM
                     PGADWVARAEAELAALPDVRIFRRTSVFGAYDGGAYGALEHVADHLPQPSTHQPRQRL
                     WQIVARRTINAMGAIERPIVFNGNDRPGVILASALRTYVNRFATTPGRKIAFFVSSDG
                     GWRAAQDILNAGLNIEIMIDSRDSVADSLTRPIARAGVRILLDAFVIGTHGHLGLKRI
                     DVRKNDKILQFDVDVLAVSNGYNPQVGLTTHLGGKPVWSDAHSAFLPGDLPPGMQVAG
                     ATAGDFGLGACLRAGTQIGNALATDLGFTGAPVDVPRADDEPSATSPIWYIEGGKGKA
                     FVDLQNDVTAKDVSIAKTEGFRAVEHLKRYTTLGMATDQGKTSSVNGQALMAHLTGRS
                     MQDAGTTRYRPPHVPVAIGALAGAHVGKHFKATRYCAGHDWALENGATMVEAGQWLRP
                     RWFTQPGETDWLESVTREVKTVRAAVGICDVSSLGKIDIQGRDAGIFLDRVYVNMFST
                     LPVGKARYGLMLREDGFVLDDGTTARLGPEEYVMSTTTGNAGKVMQHLEFCHQVLWPE
                     LDVAMVSVTEQWTQYAVAGPKSRELLKNLFGDAFDMSNEAFPFLACAEFVWGGVTARL
                     CRISFSGELAYEVAVPANYGDSLMRALMRAGAPFGVVPYGSEALGVLRIEKGHVSVPE
                     LNGQTTAADLGLGKMLSKKKNFIGRVLADRSALTDPERPGLVGLKPVDPGHRLRSGAH
                     FLPLDAQESAENDVGYMTSVAYSTLAGCWVGIGFLKRGA"
     gene            complement(2855..3121)
                     /locus_tag="VGB93_13900"
     CDS             complement(2855..3121)
                     /locus_tag="VGB93_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY0147209.1"
                     /translation="MRIPCPYCGFRDLSEFVTIGESTGPRPDPAAPNAAEKFFDYAYL
                     RTNPAGPSEEYWYHAFGCRSWLKVVRDTRTHAIESAVLAREGAR"
     gene            complement(3128..4378)
                     /locus_tag="VGB93_13905"
     CDS             complement(3128..4378)
                     /locus_tag="VGB93_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY0147210.1"
                     /translation="MHYSFLSLLKNAFTGHQGWTPTWRNAEPKPEYHAIIVGGGGHGL
                     STAYYLAKEHGLTNVAVLEKGWIGGGNIGRNTTIIRSNYLLPGNIVFYEWSMKLWEKL
                     EQDFNYNAMVSQRGVLNLYHSDGQRDAYARRGNAMRLHGVDADLLDREQVRAMLPFLN
                     FDDARFPIQGGLLQKRGGTARHDAVAWGYARGASDRGVDIVQNCEVTGLIIENGRATG
                     VETTRGPIRAKKVGLSVAGNSSRVAAFAGLRLPIESHVIQAFVSEGLKPLIPNVITFG
                     AGHFYISQSDKGGLVFGGDLDGYKSYAQRGNLPTVKDVCEGGMALMPMIGRLRVLRNW
                     GGIMDMSADGSPIIDKTPVEGLYLNTGWNYGGFKAVPAAGWCFAHLIARDETNSAAAA
                     FRLDRFARGAVLDEKGQGPQPNLH"
     gene            4450..5484
                     /locus_tag="VGB93_13910"
     CDS             4450..5484
                     /locus_tag="VGB93_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY0147211.1"
                     /translation="MSTSAISMTKGDISAGQRDEVRKLGFLLIDDFALMSFASAIEPF
                     RAANVLAGRSLYEWVAIGVHRNGAAASAGLLVQTQHPVGADLDLDTLFVCAGGNPAEF
                     ADETTLQWLRRLARRGVRIGGVSGGPFILARAGLLGGYRCTMHWEHVPAFSESFPQLA
                     LRRSLYEIDRDRLTCAGGIAALDMMHALIAAEQGRVLADKVSDWFLQTDIRLGTGAQR
                     RSLQERYDTANAHLLTALELIEGHVERPMKRRALAQAAGISVRQLERLFAHHLDTTLD
                     GHYREVQLERARTLLRQTAMPVAEVGLASGFQSASHFSRQFRQRFGHAPLQERRTKAK
                     PDVRPDRADR"
     gene            complement(5459..6130)
                     /locus_tag="VGB93_13915"
     CDS             complement(5459..6130)
                     /locus_tag="VGB93_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0147212.1"
                     /translation="MDRKTKNAIAVADDSTAAAQNLLTVSNAPPGDARSLERAIGSQI
                     RQLRRRLNLSVADLASAAQISTGMMSKIETGQISASLASLQSIAAALSAPMTSLFAAF
                     EEQRDCSFVKADQGVVIERRGSKVGHVYQLLGHALGGDVAVEPYLITLEKEAAPYTAF
                     QHAGREFIYMLSGLVDYRHGDKIYRLAPGDSLLFDSSAMHGPEALLELPMTYLSIIIY
                     QRDRA"
     gene            complement(6302..7603)
                     /gene="glnT"
                     /locus_tag="VGB93_13920"
     CDS             complement(6302..7603)
                     /gene="glnT"
                     /locus_tag="VGB93_13920"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEY0147213.1"
                     /translation="MSKDLEKIAKDRGIKYFLISFTDLFGTQRSKLVPTEAITAMAKN
                     GAGFAGFATWLDMTPADPDTFVIPDPDSLIQLPWKKEVGWLAGNPWMDGKEVEQAPRN
                     VLRRQIAAAAEQGLQMKSGVECEFFLISPAGDCISDPSDFQSKPCYDQTALMRRFDVI
                     AEICDSMLELGWKPYQNDHEDANGQFEMNWNYDDALVTADRHSFFKYMVKSIAEKHGL
                     RATFMPKPFSNLTGNGCHAHVSLWKKDENVFEDPKGELGVSKLGYHFLAGILANAEAM
                     TALTNPTANSYKRINGTVTLSGATWSPNTITYTGNNRTHMVRIPEEGRFEFRLADGAA
                     NPYLLQAALLAAGLDGLAHSRDPGKRLDINMYTHGHLVEGAKKLPLNLLDALRALEAS
                     PLFEKAFGTEFVTAYVKLKTDNWNEYMRHLSKWEVETTLDC"
     gene            complement(7945..8511)
                     /locus_tag="VGB93_13925"
     CDS             complement(7945..8511)
                     /locus_tag="VGB93_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147214.1"
                     /translation="MALKFARRWSWGPSPEPPDPQDAAPASILLASEGAPILPEVLDF
                     TAHLSRKAHAPVYVLMIARVWGSSFGLPHPGLRPTKREWQAQRDRAAEILHQLEQRGV
                     EATAAVVSTRNASGRILAEAKRRRPGAIVMAAPPPRHWLIAHFFWEQEPYRVRRLAGP
                     PVYLIVEGEAAAGAPSAFARNGANGEAM"
BASE COUNT         1660 a         2599 c         2707 g         1715 t
ORIGIN      
        1 gcgccgcgtt tgaggaatcc gatcccgacc cagcagccgg caagcgtcga ataggcgacc
       61 gaggtcatgt agcctacgtc gttctccgcg ctttcctgcg catcgagcgg caggaaatgc
      121 gcgccggagc gtaaccggtg gcccggatcg acgggcttga ggccgacgag accaggtcgt
      181 tccggatcgg tcagcgccga gcgatctgcc agcacgcgac cgatgaaatt ttttttcttg
      241 gagagcatct tgcccaggcc gagatccgcc gccgtcgtct ggccgttgag ctcgggcacg
      301 gagacgtgcc ccttctcgat gcgcaatacg cccagcgcct cgctgccgta gggcacgacg
      361 ccgaatggcg cgccggcacg catcaacgcc cgcatgaggc tgtcgccata attggctggc
      421 acggcgacct cgtaggcaag ctcgcccgaa aaagaaatgc gacagagccg tgccgtcacg
      481 ccgccccaaa cgaactcggc gcaggcgagg aatggaaagg cctcattcga catgtcgaac
      541 gcgtcgccaa acaggttctt caacagctcc cgcgacttcg gccccgcgac ggcatattgc
      601 gtccattgtt cggtgaccga caccatcgcg acatcgagct ccggccagag aacctgatgg
      661 cagaattcga gatgctgcat caccttgccg gcattgccgg tggtggtgga catcacatat
      721 tcctcgggac caagccgcgc ggtcgtacca tcgtcgagca cgaaaccatc ttcccgcagc
      781 atgagtccgt aacgcgcttt gccgacgggc agcgtcgaga acatgttgac atagacgcga
      841 tcgagaaaaa taccggcgtc gcggccctga atgtcaatct tgccgaggct cgatacatcg
      901 cagatgccga cggccgcacg caccgtcttc acttcacggg tgacgctttc gagccaatcg
      961 gtttcgccgg gctgcgtaaa ccagcgcggc cgcagccatt gtccggcttc aaccatggtc
     1021 gcgccgtttt ccagcgccca gtcatggcct gcgcaatagc gcgtcgcctt gaagtgcttg
     1081 ccaacatgcg ccccggccaa agctccgatc gcaaccggga catgcggcgg acgataccgt
     1141 gtcgtgcccg cgtcctgcat tgaacgcccg gtcaaatgcg ccatcagcgc ctgaccgttg
     1201 acgctcgacg ttttgccttg gtcagtcgcc atgccgagcg tcgtatagcg cttcaaatgc
     1261 tcaacggcgc ggaagccttc ggtcttggcg atggaaacat ctttggctgt cacgtcgttc
     1321 tgcagatcga caaaggcttt ccctttgccg ccctcgatat accagatcgg cgacgtggcg
     1381 cttggttcgt catccgcgcg cggaacgtcc acgggcgcgc cggtgaagcc aaggtcggtg
     1441 gcgagcgcgt tgccgatttg cgttcccgcc ctcaggcagg caccgaggcc aaaatcaccc
     1501 gctgtggcgc cggcgacttg catgccaggc ggcagatcgc ccggcagaaa ggcgctgtgc
     1561 gcatccgacc agacaggctt gccaccaaga tgtgtcgtga gaccgacctg cggattgtag
     1621 ccgttggaga ccgccagcac gtccacgtca aattgaagta ttttgtcgtt cttgcgcacg
     1681 tcgatgcgct tcaatcccaa atgcccgtgg gtgccgatga cgaatgcatc gagcagaata
     1741 cggacgcccg cgcgggcgat tggccgggtc agggagtccg cgacgctgtc tcgggagtcg
     1801 atcatgatct cgatgttgag gccggcgttc aggatatctt gcgccgcccg ccagcctccg
     1861 tctgacgaca cgaagaatgc gatcttgcgg ccgggcgtcg tggcaaagcg attgacatag
     1921 gtacgcaatg ccgaagcgag aatcacgccc ggtcgatcgt tgccgttgaa tacgatcggc
     1981 cgctcgatcg cgcccatcgc attgatcgtg cgccgcgcca cgatctgcca gagacgctgg
     2041 cgcggctggt gcgttgacgg ttgcggcaga tggtcggcga cgtgttcgag cgcgccatag
     2101 gcgccgccat cataggcacc gaagacggag gtgcgccgga agatgcgcac gtccggcagc
     2161 gcggccagtt cggcctcggc ccgcgcgacc caatcggcgc cgggcatgga atcgatttct
     2221 cgcgcctccg acaacagccg gccgcccagg cgaaaatctt cctcgcagat gatgacgcgt
     2281 gcgcctgcgc gcccggcggc caaagccgcg gcaagacccg ccggccctga gccgatgacc
     2341 agcacatcgc aataggccgt cgcgtgctcg taatagtcgg ggtcagggag gccgctcgcc
     2401 gcgccaagcc cggcggcgcg gcggattgca ggctcataga ccttctccca gaaggaggct
     2461 ggccacatga acgtcttgta gtagaagccg ccgacgaaga gcggcgcgaa aagctgattg
     2521 atcgagagaa tgtcgaggct gagcgttggc cagcaattct ggcttgtcgc aacgagacga
     2581 tcataaagct cgatcgtcgt cgccttggtg ttcggctcgc gccgcgcgcc atcgcgcaaa
     2641 gtcacaagcg cgttcggctc ttccggtccg gcagtgaaga tgccgcgtgg tcgatggtat
     2701 ttgaacgaac gcccgacgag cttaaccccg ttcgccatca aggcggaggc cagcgtgtcg
     2761 ccgggatggc cgaggtaatg cctgccgtcg aacttaaaag cgatctcgcg gcgtcgatcg
     2821 acgagaccgc catttggcag acggagggga ccactcatcg cgcgccctcc ctggcgagaa
     2881 cggcgctttc gatcgcatgc gtgcgggtat cacgaacgac tttcagccat gagcggcagc
     2941 caaacgcatg ataccaatat tcctcgctag ggccggccgg gttggtccgc aggtaggcgt
     3001 agtcgaagaa tttttccgcc gcgttgggcg ctgcggggtc gggacgcggg cccgtcgatt
     3061 cgccaatggt gacgaattcg gaaaggtcgc ggaaaccgca ataggggcag gggatgcgca
     3121 tggcgcctca atggagattg ggttgcgggc cttggccttt ttcgtcgagg accgcgccgc
     3181 gggcgaaacg gtcaagccgg aaggctgcgg cagcggaatt cgtttcatcg cgggcaatca
     3241 aatgggcgaa acaccagccg gctgccggca cggctttgaa gccgccgtag ttccagccgg
     3301 tgttgagata gaggccttcg accggcgtct tatcgatgat cggcgaacca tccgccgaca
     3361 tgtccatgat gccaccccag ttgcgcagga cgcggagacg cccaatcatc ggcatcagcg
     3421 ccatgccgcc ttcgcagaca tcttttacag tcggcagatt gccgcgctgg gcgtaggatt
     3481 tgtagccgtc gagatcgccg ccgaagacga gtcccccctt gtccgactgg ctgatgtaga
     3541 aatggcccgc gccgaacgtg atgacgttcg ggatcagcgg cttcagacct tccgagacga
     3601 aggcttgaat gacatggctt tcgatcggca ggcgcaggcc agcgaaggcc gcaacgcgcg
     3661 aactgttgcc ggcaacggag agaccgacct ttttcgcccg gatgggaccg cgcgtcgtct
     3721 cgacgccggt ggcgcggccg ttctcgatga tcaagcccgt cacttcgcaa ttctgcacga
     3781 tgtcgacgcc gcggtcgctg gcaccgcgcg cgtagcccca ggcgacggcg tcgtgccgcg
     3841 ccgtgccgcc gcgcttttgc aagaggccgc cctgaatggg gaagcgcgca tcatcgaaat
     3901 tcagaaatgg cagcatggcg cgtacctgct cacggtcgag caaatccgca tcgacgccat
     3961 gtaatcgcat cgcattgccg cgccgtgcat aggcgtcgcg ctggccgtcg gaatgataga
     4021 ggttcaacac gccgcgctgg ctgaccatcg cattgtaatt gaaatcctgc tccagctttt
     4081 cccaaagctt catcgaccat tcgtagaaga cgatattgcc cggcagcaga tagttcgagc
     4141 ggatgatcgt cgtgttgcgg ccgatattgc cgccgccgat ccagcctttt tccagcaccg
     4201 cgacattggt gaggccgtgc tctttggcga gataataggc ggtcgaaaga ccgtggccgc
     4261 cgccgccgac gatgatggcg tggtattccg gcttgggttc ggcattgcgc cacgtcggcg
     4321 tccagccttg atggccggtg aacgcgtttt tcagcaggga caagaaagaa taatgcatgg
     4381 cgatcgtaaa ggggcggcgg cgctgcggcc ttgactttgg ccagcatgga tgcggataaa
     4441 agtaaaatct tgtccacgag cgccatttcc atgaccaagg gcgacatatc cgccgggcag
     4501 cgcgatgagg tacgaaagct gggctttctg ctgatcgacg atttcgcgct gatgtccttt
     4561 gcgtccgcca ttgagccgtt ccgcgcggcc aatgtgctcg cgggacgcag cctctacgag
     4621 tgggtcgcaa tcggtgtcca tcgaaacggc gcggccgcgt ctgccggact cttggtgcaa
     4681 acgcagcatc ctgtgggtgc cgatctcgat cttgatacgc tcttcgtttg tgcaggcggc
     4741 aatccggctg aatttgctga cgagacgacg ttgcaatggc ttcggcgcct ggcgcggcgc
     4801 ggcgtgcgga tcggcggcgt ctccggcggc ccgttcattc tggcccgtgc cggccttctc
     4861 ggcggctacc gctgtacgat gcactgggaa catgttccgg cgttcagcga gagctttccg
     4921 cagcttgcgc tgcgccgctc gctctatgaa atcgaccgcg acaggctcac gtgcgctggc
     4981 ggcatcgccg cgctcgacat gatgcacgcg ctgatcgccg cggagcaggg gcgcgtcctg
     5041 gccgacaagg tcagcgactg gtttctgcaa actgacattc ggcttggaac gggcgcgcag
     5101 cgccgctcgt tgcaggaacg ctatgatacg gccaacgcgc atcttctgac ggcgctggaa
     5161 ctgatcgagg gccatgttga acgcccgatg aaaaggcgcg ccctagcgca ggctgccggc
     5221 atctccgtgc gccagctcga acggctcttc gcgcaccatc tggatacgac gctcgacgga
     5281 cattatcgtg aagttcagct tgaacgggcg cgaacgctct tacggcagac ggcgatgccg
     5341 gtggcggaag tgggcttggc gagcggcttt caaagcgcta gccatttctc gcggcagttc
     5401 aggcagcgtt tcggtcacgc gccgctgcag gagcgtcgga cgaaggcaaa gccggacgtc
     5461 aggcccgatc gcgctgatag atgatgatcg acagataggt catcggcagt tcaagcaaag
     5521 cctccggccc atgcatggcg ctagaatcga acagaagtga atcgcctggc gccagccgat
     5581 agatcttgtc accatgccgg taatcgacga gcccgctcag catatagatg aactcgcgtc
     5641 cggcatgctg gaaggccgtg taaggcgccg cctctttttc aagcgtgatg agataaggtt
     5701 cgacagcgac gtcgccgccc aacgcatgac cgaggagttg gtagacatgg ccgaccttcg
     5761 atccgcgccg ctcgataacg acgccttgat ccgctttgac gaaagagcaa tcgcgctgct
     5821 cttcgaaggc ggcgaagagg gaggtcattg gcgcgctcag cgccgcggcg attgattgca
     5881 gcgaggcgag ggacgccgaa atctgccccg tctcgatctt cgacatcatc ccggtcgaga
     5941 tctgcgcggc gctagcgagg tcggcgacgg aaagattgag acgccgccgc aattgccgga
     6001 tctggctgcc gatcgcccgc tcaaggctcc gcgcgtctcc cggtggagcg ttggagaccg
     6061 tcagcaggtt ttgtgcggcg gcagtggaat cgtctgccac cgcgattgcg tttttcgtct
     6121 tgcgatccat gctttcgacc ggcgaacgtg aacgtcgata agcgttaagg cttaacttgc
     6181 gcccgatatc aaccgcaagg tgcgagctac cttggtgccg atatttttac atccatccac
     6241 cgagataatg cggcaattgc gaaatttttc cagtcagatg cgctttgctg tgcccttggg
     6301 cttagcaatc aagcgtcgtc tcgacctccc atttgctgag atggcgcata tattcattcc
     6361 aattgtcggt tttgagcttg acgtaggctg tgacaaattc ggtgccgaac gccttttcga
     6421 agagcggcga agcttcgagg gcccgcaaag catcgagcag attgagcggc agcttcttgg
     6481 cgccttcgac gagatggccg tgcgtataca tgttgatgtc gagccgtttg cccgggtcgc
     6541 gcgaatgcgc gagaccgtca agacccgctg ccagaagtgc cgcctggagc aggtaggggt
     6601 tggccgcgcc gtcggcgaga cggaattcga accgcccttc ctcgggaatc cgcaccatgt
     6661 gggtgcggtt gttgccggta taggtgatcg tattcggcga ccaggtcgcg cccgatagcg
     6721 tgacggtccc gttgatgcgc ttgtagctgt tagctgtcgg attggtcagc gcggtcatcg
     6781 cttcggcgtt ggcgagaatg ccggcgagga aatggtagcc aagcttcgag acgccaagtt
     6841 cgcctttcgg atcctcgaaa acattctcgt ccttcttcca aagcgaaacg tgcgcgtgac
     6901 agccgttgcc ggtgaggttc gagaagggct ttggcataaa ggtcgcgcgc agcccatgct
     6961 tttcggcgat cgacttcacc atgtatttga agaacgaatg tcggtcggcc gtcacaagcg
     7021 cgtcatcgta attccagttc atttcgaact ggccgttagc gtcctcgtga tcgttttgat
     7081 agggtttcca gcccagttcg agcatgctgt cgcagatttc cgcgatcaca tcaaaacggc
     7141 gcatcaaagc cgtctgatcg tagcagggct ttgactggaa atcgctgggg tccgaaatgc
     7201 aatcaccggc tggcgagatg aggaagaatt cgcattcgac gccggatttc atttgcaggc
     7261 cctgttcggc cgcggcggcg atctgacggc gcagaacgtt gcgcggcgcc tgttcgactt
     7321 ccttgccgtc catccaggga ttgccggcga gccagccgac ttctttcttc catggcaatt
     7381 ggatgaggct gtcgggatcc gggatcacga acgtatcggg gtcggctggc gtcatatcaa
     7441 gccaggtggc gaagcccgca aaccccgcgc cgttcttggc catggcggtg atggcttccg
     7501 tcggcacaag cttggagcgt tgcgtgccga acagatcggt aaacgagatc aaaaagtatt
     7561 tgatgccgcg atctttcgcg atcttctcga gatcttttga catatccttt tcctatccca
     7621 agcttcggtt attagcgttc agctcgcccg ctttgtcgct ccgggcaatg cgttaatgtc
     7681 cgttgccgcg gcgcacgctg cagccatgat ttcgcagaga cgggatatca aggccggcat
     7741 taactgccac ttccacgggg ttgatttcgt cgaaccagtg aaaggcgctt cctggatgtc
     7801 aggaacccag cggtgcagcg catacttttg atctcctcca tcatggcacg gtattggcaa
     7861 aaattattcc cgtcaactaa agggaactaa tttttcgtca taggaaatta ttggcgtttc
     7921 ccgtcagtga gagctgggaa ggattcacat ggcctcgcca tttgctccat tccgcgcgaa
     7981 agcagatggc gcgccagccg cagcttcacc ttcgacaatg aggtagacgg gtggcccggc
     8041 caagcgacgc acccggtaag gctcctgttc ccagaagaaa tgggcgatca gccaatgacg
     8101 cggtggcggc gctgccatga cgatagcgcc gggtctgcgc cgtttggcct ctgctaaaat
     8161 ccgccctgat gcgttgcgcg tgctgacgac agcggcggtg gcctcgacac cgcgctgctc
     8221 caattggtgc aatatttcag cggctctgtc gcgctgcgcc tgccattcgc gtttggtcgg
     8281 cctcaaacct ggatggggta ggccgaaact gctgccccaa acgcgcgcga tcatgagcac
     8341 atacaccggc gcatgtgcct tccgcgatag atgcgcagtg aagtccaaaa cctctggcaa
     8401 tatcggtgcg ccctccgagg cgaggaggat ggaagccggc gctgcgtctt ggggatcggg
     8461 cggttccggc gacgggcccc aggaccagcg ccgtgcgaat ttcaatgcca tggcgcaaat
     8521 caaaacgagg ttgatgaaag attcgatcag cagcctgtag gctgctgatc gtcttgatct
     8581 acgtctcgcc ctgcttagga cgtcgcactg gcttccatcg ccggctttcg cttgtcttcg
     8641 tacttccggt accaatagag cgggaggtaa gcgaggagcg t
//
LOCUS       DASWOG010000676         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_346244,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000676 DASWOG010000000
VERSION     DASWOG010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346244"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGB93_13930"
     CDS             complement(<1..941)
                     /locus_tag="VGB93_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0147215.1"
                     /translation="MLEKTENLNHTTPLQAAPEVDTAQKRGSGRRPFVLLAVLLLLLG
                     GIYYGIHSRTSAESALAQETITSSVPNVHVVPPQENAPSQGLVLPGQTTAFTDTPIYA
                     RSSGYLKKWYADIGAQVKKGELLAEVETPELDAQLRQARADLVTAQANAKLADITANR
                     TQDLLKSQSVSTQERDNAAGALAADQAIVSSRQAEVARLEELQGYEKIYAPFDGVITA
                     RNTDIGDLITPGPGTLNSELFREQATKVLRIYVAVPEVYTPQIHVGEKPKITLDEYPN
                     QAFEGTLVRTDRAINQQSLTLLVEVDVDNSEGKRLPGA"
     gene            complement(941..>3582)
                     /locus_tag="VGB93_13935"
     CDS             complement(941..>3582)
                     /locus_tag="VGB93_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0147216.1"
                     /translation="INFDPRELQAKGLSPANVLAVVQQQYTVLPSGTAKISEFEYDVH
                     INGTPMLADALNNIPIKTTGNATIYLRDVATVSMGFAPQTNVVRQNGQRGVLVSIVKA
                     GNASTISVVNGIKDLLPRVAQTLPPQLKIQPLGDQSVFVKASVFGVIREAVIAAGLTA
                     MMILLFLGSWRSTLIIAVSIPLSILTSVMVLSFIGQTINIMTLGGLALAVGILVDDAT
                     VTIENIERFMEEGHPQRDAILYGAAQIAVPALVSTLSICIVFVPMFLLHGVARYLFIP
                     LAEAVVFAMIASYILSRTLVPTMAMYLLTTEKHANAGRRTRNPLKMFQQGFEARFERL
                     RLGYRDLLTSFVHNRKTFIVVFLGCVFAVFLLAPWLGQDFFPNTDSGQFTLHMRAKTG
                     TRIEDTARIADLVENRIRDIIPSHELVSILDNIGLPYSGINLTHTSTGVIGAADADIM
                     VQLSEDHHPTDDYVQKIRLAVAREFPSVIFYTLPADMITQILNFGLPAPIDVQITGSD
                     IQKNRIVADRMLERIRAVGGIVDAHIQQEFDYPMFQISVDRTKAQQNGLTESDVASSV
                     LETLSGSFQTAPLFFLNFKTGVNYNLAAMAPQYDIQSLQDLQNLPITGPGQRAPAILA
                     DVATITRSQEMAAVDHYNIRRVVDIYANVRGRDLGAVGTDLEKIVDQDRKLLPRGSFV
                     ELRGQLTTMRSSYMDLFEGLVFSILFVYLLIVVNFQSWSDAFIIITALPAALAGIVLF
                     LFFTGTTLSVPALMGAIMCMGVATANSILVIAFAREKLAEHGDAIQAAIDAGFTRFRP
                     VLMTALAMIIGMVPMALGLGEGGEQNAPLGRAVIGGLMLATVATLIFVPTVFALLHNR
                     RGGSGPLRPEPPGEHAGHGSLVA"
BASE COUNT          791 a         1019 c         1057 g          715 t
ORIGIN      
        1 gctccgggta aacgcttgcc ttcagaattg tcgacatcga cctcaacgag caacgtgagg
       61 gactgctgat tgatggccct gtcggtacgc acgagcgtac cttcaaaagc ttggttcggg
      121 tattcgtcga gcgtgatctt cggcttttcc cccacatgaa tctggggagt ataaacctcg
      181 gggaccgcca catagattcg cagcactttg gtcgcctgct cgcggaacaa ttcgctgttg
      241 agcgttccag gccccggggt gatgagatcg ccgatatcag tgttgcgggc agtgatcacg
      301 ccgtcaaagg gcgcgtagat cttttcgtag ccctgcaact cttcgagacg cgcgacctca
      361 gcctgtcgcg aagaaacaat cgcttggtcg gcagccaagg cgccagcggc attgtcacgc
      421 tcctgcgtcg aaaccgactg cgacttcagc aaatcctggg tacgattcgc cgtaatgtcc
      481 gccagctttg cattggcttg cgcggtgaca agatcggcgc gcgcctgtcg caattgcgcg
      541 tcgagctctg gcgtctcgac ttcggcgagc aattcgccct ttttaacttg cgcgccgatg
      601 tcggcgtacc atttttttag atagccgctg gagcgcgcgt agatcggcgt atccgtgaag
      661 gccgtggttt ggccgggcag aacaaggccc tgactcggcg cgttctcctg cggcggaacg
      721 acatggacat tcggcaccga agacgtgatc gtttcctgtg ccagcgcgga ctcggccgaa
      781 gtccgcgaat ggatgccgta ataaatgccg ccgagcagta agagcagcac cgcgagaagc
      841 acgaacggcc gccttcccga tccgcgcttc tgcgcggtat ctacctccgg cgccgcctga
      901 agaggggtgg tgtgattcaa attttcggtt ttctcgagca ttaggccact aagctcccgt
      961 gccctgcgtg ttcacccggc ggttcgggcc tcaacggtcc cgagcccccc ctgcgattgt
     1021 gcagcaatgc aaatacagtc ggtacgaata tgagcgttgc gacggtcgcg agcatcagcc
     1081 cgccgatcac cgcgcggccg aggggtgcat tttgttcgcc accttcgccc agccccaaag
     1141 ccattggcac catgccgatg atcatggcga gagcggtcat aaggactggc cgaaaccggg
     1201 tgaagcctgc atcgatcgcc gcctggatcg catcaccatg ctccgccagc ttctcgcgcg
     1261 caaacgcgat gacgagaatg gagttcgccg tggcgacgcc catgcacatg atagcgccca
     1321 tcagagccgg gaccgacagc gtcgttccgg taaagaacaa gaacagaacg ataccggcta
     1381 gtgccgccgg caacgccgtt atgatgataa aggcatccga ccaagactgg aagttgacga
     1441 cgatcagcag gtaaacgaac aaaatggaga atacgaggcc ttcgaaaagg tccatgtaag
     1501 aggagcgcat tgtcgtcaat tgtccgcgaa gctccacgaa gctgccgcgc ggcagaagct
     1561 tcctgtcctg atcgacgatt ttctctaggt cagttccgac agcgcccaaa tcgcggccgc
     1621 ggacattggc gtaaatatcg acaacacggc gaatattata atgatcgacc gccgccattt
     1681 cctgtgagcg cgtaatggtt gcgacatccg cgaggatcgc cggcgcgcgc tgccccggcc
     1741 ccgtgatcgg cagattttgc agatcctgca gggactgaat gtcatattgg ggcgccatcg
     1801 ccgcaagatt gtagttcaca ccggtcttga aattgagaaa gaacagcggc gcggtctgaa
     1861 agctcccgct caatgtttcg aggacactgc tcgcgacgtc gctttcggtg agaccgtttt
     1921 gctgagcctt tgtgcgatca accgaaatct ggaacatcgg ataatcgaat tcttgctgga
     1981 tatgcgcatc gacgataccg cccacggcac ggatacgctc aagcatacgg tcggcgacga
     2041 tgcgattttt ctggatatca gacccggtga tctgaacatc gatcggcgcc ggcaggccga
     2101 aattgaggat ttgcgtgatc atatcggccg gcaacgtata gaagatgacc gagggaaatt
     2161 ctctcgccac ggccagcctg atcttctgaa cataatcatc cgtcggatga tggtcctcgc
     2221 tgagctggac cataatgtcc gcatccgccg ccccgatcac acctgtggag gtgtgggtga
     2281 ggttgatgcc gctatagggc agcccgatat tgtcaagaat cgagacgagt tcgtgtgacg
     2341 ggatgatgtc acgaatccgg ttctcgacga gatcggcgat ccgcgcggta tcctcaatac
     2401 gcgtgcctgt cttcgcccgc atatgcaagg tgaactggcc gctatcggtg ttggggaaga
     2461 aatcctggcc gagccaaggc gccagcaaga aaaccgcaaa cacgcagccg agaaaaacga
     2521 cgatgaaggt tttgcggtta tgcacgaacg aagtcagaag atcgcgatag ccgagccgaa
     2581 gccgctcgaa ccgcgcctcg aacccttgct ggaacatttt caggggattg cgtgtccggc
     2641 gccccgcatt agcgtgcttc tccgtcgtca ggagatacat ggccatcgtc gggacgagcg
     2701 tacgcgacaa tatataagat gcgatcatgg cgaagacgac cgcttccgcc aatggaatga
     2761 acagatagcg cgcaacgccg tgcaacagga acatcggcac gaagacgata cagatcgata
     2821 gggtcgagac gagggccggc acggcgatct gagcagcgcc ataaagaatg gcatcacgtt
     2881 gcggatgccc ctcctccatg aagcgctcga tgttctcgat cgtcacggtc gcgtcgtcga
     2941 cgagaatacc gaccgcaagg gccaaaccgc cgagcgtcat aatattgatg gtctggccga
     3001 tgaagctcag caccatgacc gaggtcagaa tcgaaagcgg gattgaaacc gcgatgatga
     3061 gagtcgagcg ccagctgccc aggaacagca gaatcatcat cgctgtgaga cccgccgcga
     3121 tcaccgcttc gcggatgacg ccgaagacgg acgccttcac aaaaaccgat tgatcgccca
     3181 gaggctggat tttcaattgc ggcggcagag tttgggcgac gcgcggcagc agatctttga
     3241 tgccgttgac gacgctaatg gtcgaggcgt tgccggcctt gacaatgctc acgagcacgc
     3301 cgcgctggcc attttggcgc acgacgttgg tttgcggcgc gaagcccatg ctcacggtcg
     3361 cgacatcccg cagatagatt gtcgcattcc cggtcgtctt gattgggatg ttgttaaggg
     3421 catccgccag cattggtgtg ccgttgatat gcacgtcgta ttcgaattcg gagatcttcg
     3481 ctgtgcctga cggcaacacg gtatattgct gctggacgac agccagcaca ttggccggcg
     3541 aaagcccctt ggcctgaagc tcccggggat cgaagttgat ga
//
LOCUS       DASWOG010000677         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_255303,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000677 DASWOG010000000
VERSION     DASWOG010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255303"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1205)
                     /locus_tag="VGB93_13940"
     CDS             complement(327..1205)
                     /locus_tag="VGB93_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0147217.1"
                     /translation="MTELDAGKSGTFSIDGKVEVHRLGFGAMRVTGPGIWGPPQDRAE
                     ALRTLRRLPELGINFIDTANSYGPDVSEELIREALYPYKGLFIATKAGLERPGPDNWK
                     PNGKPDYLRAEAHKSLKKLGVERIDLWQLHRIDPSVPREDQFALIKSLLDEGVIAHAG
                     LSEVSVEEVKAAQKVFKVATVQNRYNLVDRGSEEVLDYCEAEGIGFIPWFPLAAGKLT
                     QDGSVLDKIAKKHGHAPGQVALAWLLKRSPVILPIPGTSKVKHLEENVSAVEISLSDE
                     EFATLDRAGKAESGKG"
     gene            1431..1673
                     /locus_tag="VGB93_13945"
     CDS             1431..1673
                     /locus_tag="VGB93_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147218.1"
                     /translation="MPIQLPLKIIAAAVPLLFAAAAHAQTQAATTNENAPADANSGVQ
                     AQQDPVGPTHADGKAGKSSRPWFSQESEPDLHAVPR"
     gene            complement(1683..>3295)
                     /locus_tag="VGB93_13950"
     CDS             complement(1683..>3295)
                     /locus_tag="VGB93_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0147219.1"
                     /translation="GNIGDETRPIVPSETNFRAAADRLAWLGRAAIDLDIMIAVEIDG
                     VAAEPRQAIGSGTKIRLGRHNGPSFIADITHAASDDLGMTGAQQKQVSVRRVIRRDVR
                     VAIGLVALAVFAAVGTLIYNARLSQQIRQRLDRAAFLVEIRHQAGGCPDWDATAWPVS
                     RDLLVTNAHVVDEAGQGNGQGTMLVRAPGADGKEYEVIGHILHPGYKAFADFFRRQDL
                     VKWDDGQLETPPTGLAYDVALLRVKGPLPEDAILPLASRREAQSLWKGMQIESEGYPS
                     QNVSGSSVLGCGATPKWRAGAIESLTDYFSSAQQNPLETLLVTHNIPITCGASGSPII
                     GEDGRVVAVISAFSSTGGECTPSAAQINYAQRADLVADLLDGRADDALARDRQYWTSQ
                     LALLREAGYREGDAIYISLILQADKTNAGIDATTKPALDVRAPMTLAADAKSGGATGS
                     TPLKLVAGANYVIIAYAQDETPIALTLRDASGAAVAFTKPLSSDSVWYPSLMYTSKSG
                     GEYQAIVSGAPAGRQYMLRLYRWAPPQP"
BASE COUNT          652 a         1040 c          953 g          650 t
ORIGIN      
        1 agtaggaaat ggcaaggctg cgggaaagcc acgaggtgtt cgcggggctg gcaatgcgca
       61 caatctgatt catgaacgct cacaagacgc agagaacaga gaatgaaatt gcagggatca
      121 aggttccctt ttgtaggaag aggtctaatc cttttcgcat gaacggcgcc tgtacggcgc
      181 cgcacctaaa taatttgtca tctctcaagc aacgattggg ccatttccgc gtagaaatca
      241 tggcaagaaa acggtgagcg aaccggccgg ttcgctcacc gtcatcaaac catgcggaaa
      301 attttacgaa gcgccgccct gaacaatcag cccttgccgg attcagcctt gccagcgcgg
      361 tcgagtgtgg cgaattcctc atccgacaga ctgatctcga cagccgagac attctcctcg
      421 aggtgcttga ccttcgacgt gcccggaatg ggcagtatca ccgggctccg cttcagcagc
      481 caggcgagcg ctacctggcc cggtgcgtgg ccgtgcttct tggcgatctt gtcgagcacc
      541 gagccgtcct gggtcagctt acctgccgcg agcggaaacc aggggatgaa accgatccct
      601 tccgcctcgc aataatccag aacctcctcg ctgccgcggt cgacgagatt gtagcggttt
      661 tgaacagtgg cgactttgaa caccttctgc gccgccttga cctcctcgac cgagacctcg
      721 ctgaggccgg cgtgcgcgat cacgccttca tcgaggagcg acttgatcaa cgcaaactga
      781 tcttcacgcg gcacgctcgg atcaatccgg tgtaattgcc aaaggtcgat acgttcgacg
      841 cccagcttct tcaggctctt atgcgcctcg gcgcgcaaat aatcgggctt gccgttcggc
      901 ttccaattgt ccgggcctgg ccgttcgagg ccggccttcg tcgcgatgaa aagcccttta
      961 taggggtaca gcgcctcgcg gatcagctct tccgagacgt ccgggccgta ggaattggcc
     1021 gtatcgataa aattgatgcc gagttccggc aggcgccgca aagtccgcaa cgcctccgcg
     1081 cggtcttgcg gcgggcccca aatgcccggc ccggtcacgc gcatagcgcc aaacccaagc
     1141 cgatggactt caactttgcc atcgatgctg aatgtgccgg atttcccggc gtcgagttct
     1201 gtcatgcgtc gctccatggt gtttcgccca ccatacctag agccgcggcg ccgatttgcc
     1261 agcccggggc gcaaactcca ctgcggatca acaggctttt gcgtctcggc tgaccgtatt
     1321 ccgggttcgc cttgcggcgt tgcactttct tgcaagccct aaccttgacg ctcccaaggg
     1381 tgcatgccac ctaaatagac acgtcatcgt acagactccg gatggatttc atgcccattc
     1441 aattgcctct caaaatcatt gcggccgccg tgccgttgct tttcgcggcc gcggctcacg
     1501 cccaaacgca ggccgccacg acaaacgaaa atgccccggc cgatgcaaat tctggcgtgc
     1561 aggcgcagca agatccggtt ggtccaacac atgcggatgg aaaagccggc aaaagctcgc
     1621 gtccctggtt tagccaggaa agcgagcctg atctccacgc cgtaccgcgc tgatctttcg
     1681 gctcacggct gaggcggtgc ccagcgataa aggcgcagca tgtattgcct ccccgcaggt
     1741 gcgccgctga caatcgcctg atattcgccg ccgctcttcg acgtgtacat cagcgatggg
     1801 taccagacac tgtctgacga gagcggcttg gtgaaggcga cggctgcgcc gctcgcatcg
     1861 cgcagtgtta acgcaatcgg cgtttcatct tgcgcataag cgatgatcac gtaattcgcc
     1921 ccggcgacga gcttcaatgg cgtcgagccc gtcgcgccgc ccgacttcgc gtcagcagcc
     1981 aatgtcatcg gggcgcgaac atcgagcgcc ggtttcgtgg tggcgtcgat gccggcattt
     2041 gtcttatctg cctgcaggat gagcgagatg taaatcgcat caccctcccg atagccagcc
     2101 tcccgcagca gtgccaattg cgacgtccaa tattgccggt cgcgcgccag cgcatcgtcg
     2161 gcgcggccat cgagaagatc ggcgacgaga tcggcacgct gcgcgtaatt gatctgcgcg
     2221 gcgctcggcg tgcattcgcc acccgtcgaa ctgaaggcac tgatcactgc gacgaccctg
     2281 ccatcctcgc cgatgatcgg actgccgctg gcaccgcaag tgatcgggat gttgtgagtg
     2341 acgagcaggg tttcgagcgg gttttgctgc gccgacgaga aataatcggt caggctttcg
     2401 atcgcgccgg cacgccattt cggcgtcgcg ccgcagccga gaacggagga gcccgaaaca
     2461 ttctggctcg gatagccctc gctttcgatc tgcatgccct tccacaacga ttgcgcttca
     2521 cgacgcgagg cgagcggcag gatcgcatcc tctggcaacg ggcctttcac gcgtaacagc
     2581 gcgacatcgt aggcaagtcc ggtcggcggc gtttcaagct gcccgtcatc ccatttcacc
     2641 agatcctggc gccggaaaaa atcggcgaag gctttatagc cgggatgcag gatgtggccg
     2701 atgacttcat attccttgcc gtcggcgccg ggcgcacgca cgagcatcgt cccttgcccg
     2761 ttcccctgcc cggcttcgtc cacgacatgc gcattggtta cgagcagatc gcggctgacc
     2821 ggccacgcag tcgcatccca atccgggcat cccccggcct gatggcggat ttcgacgagg
     2881 aacgcggcgc gatcgagccg ctggcgaatt tgttggctca gccgtgcgtt atagatcagc
     2941 gtcccgacag cagcgaaaac ggccagcgca acgaggccga tcgcaacgcg cacatcacgg
     3001 cggatgacac ggcgtacgga aacctgcttt tgctgcgcgc cggtcatacc gagatcatcg
     3061 cttgccgcat gggtaatatc ggcgatgaaa ctcggcccat tgtgccgtcc aagacggatt
     3121 ttcgtaccgc taccgatcgc ttggcgcggt tccgctgcga caccgtcgat ctctacggcg
     3181 atcattatgt cgagatcgat ggcggcgcgg ccgagccacg ccagacgatc cgcagcggcg
     3241 cgaaaattcg tctcggacgg cacaatgggc cgagtttcat cgccaatatt accca
//
LOCUS       DASWOG010000678         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_163072,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000678 DASWOG010000000
VERSION     DASWOG010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163072"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="ppx"
                     /locus_tag="VGB93_13955"
     CDS             complement(<1..1330)
                     /gene="ppx"
                     /locus_tag="VGB93_13955"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY0147220.1"
                     /translation="MNLQMPKLFATMQLRPEVKGPVAVVDIGSNSVRLVVYDGLTRSP
                     TPIFNEKALCALGHGVAKTGELAASSVAKALAALKRYRVLIKIMNVQQVYAFATAAAR
                     DAANGPAFVADASNAIGAPVSLLSGRREAELSALGVISGFHEPDGIVADLGGGSLELI
                     DIKGAKVGKGITLPLGGLSLMDASNHSLRNAARIAQEALEDAKLLARLRGRTLYAVGG
                     TWRALAKLHMRQRNYLLSIMHGYNIPAREAADFAGLVERVNAEALIAVEAVTPARRPL
                     LAYGAIVLDEIIRKAPPKQVTISASGVREGLLYEQLSSAEQRLDPLIFAAQTFNDAYA
                     RSPRHAEELRQWTDRFMDSLRFDETLEDKRLRHAACLLADVNWRAHPDYRSEQSLNIV
                     VNGAFTGIDHPGRCFLALVMSYRYLGLDADVNPQVRAHVSPRLLDQARLLA"
     gene            complement(1327..>1728)
                     /locus_tag="VGB93_13960"
     CDS             complement(1327..>1728)
                     /locus_tag="VGB93_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY0147221.1"
                     /translation="NIRVKSIIGRFLEHARVYAFGAGHGLPHSKAAVYISSADLMPRN
                     LDRRVESLVPITNPTCHSQVLDQIMIANLIDNQQSYRVLPDGSSTRINAGKEEPFNAH
                     SYFMTNPSLSGRGKSLQESAPKTLLKRLEGR"
BASE COUNT          348 a          514 c          542 g          324 t
ORIGIN      
        1 ccgcgagcag tcgcgcctga tcgagaagcc gcggcgaaac atgcgcgcgc acttgcgggt
       61 tcacgtcggc gtcgaggcca agataacgat aagacatgac gagcgcgagg aagcagcgtc
      121 ccggatgatc gatgccggtg aaagcaccgt tcacaacgat attcagactt tgctcgctgc
      181 gataatcggg atgcgcacgc caattgacat cggcgagcag gcaggccgca tgccgaagcc
      241 gtttgtcctc aagcgtctcg tcgaaacgca acgagtccat gaagcggtca gtccattgcc
      301 gcagttcctc ggcgtgacga ggcgagcgcg cataggcatc gttgaacgtc tgcgcggcga
      361 aaatcagcgg atcgagacgc tgctcggcgc tcgacaattg ttcgtacagc aagccctcgc
      421 gcacgccgga ggcggagata gtcacttgtt tcggcggcgc cttgcggatg atttcatcga
      481 ggacaatcgc gccataggca agcaagggac gccgcgccgg cgtcaccgcc tcgacggcaa
      541 tcaacgcttc ggcattcacg cgctcgacaa ggccggcgaa atcggcagct tcccgcgccg
      601 gaatattata gccatgcatg atgctcagca gataattgcg ctggcgcatg tgcaatttgg
      661 cgagcgcgcg ccaggttccg ccgacggcat agagcgtacg gccgcgcagt ctggcgagca
      721 gcttggcatc ttccagagct tcctgagcga tgcgcgcggc gttgcgcagc gagtggttgg
      781 acgcatccat cagcgatagg ccgccgagcg gcagcgtgat gcctttgccg accttcgcgc
      841 ctttgatgtc gatgagttcg agcgaaccgc cgccgagatc ggccacgatg ccgtccggtt
      901 catgaaagcc tgaaatgacg ccgagcgcgg aaagctccgc ctcgcggcgc ccggaaagca
      961 agctgacagg cgcaccgatc gcgttgctag cgtcggcgac aaaggctggc ccatttgccg
     1021 catcgcgcgc cgcagcggtg gcgaaagcat aaacctgttg gacgttcatg atcttgatca
     1081 gaacgcggta gcgcttgagc gcggcgagtg ccttcgccac actcgacgct gccagttcgc
     1141 ctgtcttggc cacgccgtga cccaaagcgc agagcgcctt ttcattgaag atcggcgtcg
     1201 gcgatcgcgt cagcccgtca taaacgacga gccgtaccga gtttgaaccg atgtcaacga
     1261 cggcgaccgg tcccttgacc tccgggcgca attgcatcgt tgcaaagagt ttcggcattt
     1321 gcaggttcac cggccctcca gacgtttgag cagggttttg ggcgctgatt cctgcaacga
     1381 cttgccgcga cccgagaggc ttggattggt catgaaatag gaatgcgcgt tgaacggttc
     1441 ttccttgccc gcgttgatcc gcgtgctcga cccatctggc agcactctgt agctctgctg
     1501 attgtcgatg aggttcgcga tcatgatctg atcgagcacc tggctgtgac aggtcggatt
     1561 ggtgatcggc accaaagact cgacgcggcg gtcgagattg cgtggcatca gatccgcgga
     1621 tgaaatatag accgccgcct tcgagtgtgg cagcccgtgg ccggcgccaa aagcgtagac
     1681 gcgcgcatgt tcgaggaagc gcccgatgat cgatttcacc cggatatt
//
LOCUS       DASWOG010000679         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_201218,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000679 DASWOG010000000
VERSION     DASWOG010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201218"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1136)
                     /locus_tag="VGB93_13965"
     CDS             complement(162..1136)
                     /locus_tag="VGB93_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0147222.1"
                     /translation="MTDPTDNSGTDPLLNKALDWIALLKGGEPARTDIEALQRWRAES
                     PAHEEAFRRAVEIWRNLSAASEELAAQERVVPARPFRRVSTRRAVIGGALAASAAGYM
                     AVRPPLDLWPSLKELSADYRTGKGEQRVVALAENVSLKLNTLTSIVVRSTPKEPRIEL
                     ISGEVAVDARLAGKAPLVVFAATGRATAENAAFNVRCIDGNVAVSCLRGIADVEAGGR
                     SVEVAPDRQVSYASGLGPGIAVAIDPTLATAWQTGQLIFRDRSLSEVVGEVNRYRPGM
                     IIITNSDLARRLVNGTFQISELGNFVAQVEQLFGAKATSLPGGIVLLG"
     gene            1499..>1699
                     /locus_tag="VGB93_13970"
     CDS             1499..>1699
                     /locus_tag="VGB93_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147223.1"
                     /translation="MESAKCPAEWRLKKDFAASSGPGAWPAEALDQVTPDAVGAMLQG
                     ATEIGLDSAQADLQAVRNLLRRR"
BASE COUNT          393 a          504 c          455 g          347 t
ORIGIN      
        1 agcatatgtc atcgttcgct gataataaaa attgtaatcg atcgacaaca tcgatatatt
       61 attcattaaa atcaatatgt tgctttagcg agatcatgga gcttcaacgc aatctcgatc
      121 tatgcttccg cccctttgga cattgctgat tgcaaatgct tttagccgag caacacaatt
      181 ccgcccggca aggacgtcgc tttcgccccg aataattgtt cgacctgagc cacgaagttt
      241 ccgagttcgg agatctgaaa tgtgccgttt accagccgtc gcgccaaatc gctattggtg
      301 atgataatca tccctggccg gtagcgattg acttccccga ccacttcgct gagcgatcga
      361 tcccgaaaaa tcaactggcc ggtctgccag gccgttgcca gtgttggatc gatcgcaacg
      421 gcaatgccgg gcccaagacc ggatgcataa gaaacctgcc tgtccggggc aacttcaacg
      481 ctgcgaccgc cggcctcgac atctgcgatg ccgcgcaggc aggatacagc gacattgccg
      541 tcgatgcagc gaacattaaa cgccgcgttc tccgccgttg cgcggcctgt cgccgcaaac
      601 acaacgaggg gcgcttttcc ggcaagacgt gcgtcgaccg ccacttcacc cgaaatcaat
      661 tcgatccgtg gctccttcgg ggtcgagcgt accactatgc tcgtcagcgt attcaatttg
      721 agagagacat tttccgcgag cgcgaccacg cgttgctcgc ctttgccggt gcgataatcg
      781 gccgacaatt ctttgagtga tggccataaa tccagaggag gccggacggc catataacct
      841 gccgcggaag cggccagggc tccgccaatg accgcacgcc gcgtcgaaac gcgccggaaa
      901 ggtcgtgccg ggaccacgcg ctcttgcgcc gcaagctctt ccgatgccgc gctgagattg
      961 cgccagatct cgacagctct tcgaaacgcc tcctcatgcg cgggactttc ggcacgccag
     1021 cgctgcagag cctcaatgtc ggttcgtgca ggctctccgc ccttcaaaag ggcgatccaa
     1081 tcgagcgcct tgttcagaag cgggtcggtt ccggaattat ccgtgggatc agtcacggcg
     1141 caaatttacc gttgcacgag ccaaaccatg cccctcctcc acggtcaaag gcgcaggcgt
     1201 aagagatacc tgtaacaaac gcgcttcagt ccgaagtgtc ctttgcggac tctcagaaaa
     1261 tccccgcgct agattgcgaa aacaagatct tctgaatttg gacaaagccg aatcattgcc
     1321 cgtacaatag cagagcagcc gcaatccaag actgaatctt tttttactcc gaattggatc
     1381 cgcagaacga tctgccttct catagtagac gtttgacaac gagtgatccc gcaacaaaaa
     1441 aatagatagc cgcgcagcag aaggatcgat gcgacagcgg atgagttcgg agggtgaaat
     1501 ggaatctgcc aagtgtccgg cggagtggag gctaaaaaaa gacttcgccg cgtcctcagg
     1561 acctggggcg tggcccgccg aggcgctgga ccaagtcacg cccgatgcgg tcggcgcaat
     1621 gcttcaaggc gcgactgaga tcggcctcga tagtgcgcag gctgacctgc aggcggtccg
     1681 caacctcttg cgccgccgc
//
LOCUS       DASWOG010000680         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_126657,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000680 DASWOG010000000
VERSION     DASWOG010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126657"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VGB93_13975"
     CDS             <1..513
                     /locus_tag="VGB93_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEY0147224.1"
                     /translation="GLPRASSLVFKFPERGENKTGPMPIYGLRGDLPVRFAPNGGAVP
                     GDRIVAILTPGEHITIYPIQSPDLTAFDDQPERWLDVRWDLDAGKRNLFPARILVTAI
                     NEPGTLGLVAMTIGEMGANIDTISIHAISPDFREITVDLEIPDLKHLNGLITELRTKP
                     VVSKVERVNG"
     gene            510..1271
                     /locus_tag="VGB93_13980"
     CDS             510..1271
                     /locus_tag="VGB93_13980"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0147225.1"
                     /translation="MKASILRLGVNIDHVATLRNARGGSVPDPLRVALLAIEAGADGI
                     TAHLREDRRHIRDDDIARLKAHISKPLNFEMAATDEMVKIALATRPHAACLVPERREE
                     RTTEGGLDVVGGHDHLKRISAELGRAGIVVSLFIEPEPKAIEAALSIGAPVVELHTGA
                     WCNAAAEGNTIRAATEFARLKRATETAAAFGIECHAGHGLDYDTVRQISALPRVVELN
                     IGHFLIADAIFIGLGPAIHKMHAAMTAGRAGAIVL"
     gene            1268..1690
                     /gene="acpS"
                     /locus_tag="VGB93_13985"
     CDS             1268..1690
                     /gene="acpS"
                     /locus_tag="VGB93_13985"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0147226.1"
                     /translation="MIVGFGIDLCDIRRVEEILEKHGKRFVERCFTEVERGKSDRRAA
                     RAASYAKRFAAKEACAKALGTGLTHGVFWRDMGVVNLPSGKPTLHLTGGAAARLAILL
                     PAGHMAVIHLTLTDDYPLAQAQVIIEAVKSSGPNTEPP"
BASE COUNT          374 a          581 c          618 g          380 t
ORIGIN      
        1 ggattgccgc gcgcctcaag tcttgttttt aaattcccgg agcgtggcga aaacaaaacc
       61 ggaccgatgc ccatttacgg cttgcgtggc gatctaccgg tgcgttttgc gcccaatggg
      121 ggcgccgtgc cgggcgaccg catcgtcgcg atcctgactc ccggcgagca catcacgatc
      181 tacccgattc agtcgccgga tctgacggcg ttcgacgatc agccggaacg ctggctcgac
      241 gtgcgctggg acctggacgc cggcaagcgc aacctgtttc cggcgcgcat tctcgtgacg
      301 gcgatcaacg agccaggaac cctgggcctc gtcgcaatga caatcggcga aatgggggcg
      361 aatatcgaca cgatctcgat ccacgcaatc tcgccggact ttcgcgagat caccgtcgat
      421 ctcgaaattc ctgatttgaa acatctcaac gggcttatta ccgagttgcg caccaagccg
      481 gtcgtcagca aggtcgagcg ggtgaatgga tgaaagcttc gattctacgg ctcggcgtca
      541 acatcgatca tgtcgcgaca ttgcggaatg cccgcggcgg cagcgtgccc gatccgctgc
      601 gcgtcgcgct attggcgatc gaagcgggcg ccgatggcat caccgcgcat ctgcgcgagg
      661 atcgtcggca tatccgcgac gacgatattg cgcggttgaa ggcgcacatt tccaagccgc
      721 tcaattttga aatggcggcg acggatgaaa tggtcaaaat cgcactggcg acgcgcccgc
      781 acgccgcctg ccttgtgccc gaacggcgcg aagaacgcac gacggaaggc ggccttgatg
      841 tcgtcggtgg ccacgaccat ctgaagcgca tcagtgccga attgggccgt gccggtatcg
      901 tcgtttcgct tttcatcgag ccggaaccaa aggcgatcga agccgcgctt tcgatcggcg
      961 cgccggttgt cgagctgcac accggcgcct ggtgcaatgc cgcggctgag ggcaacacaa
     1021 ttcgcgccgc gactgaattc gcgcgcttga agcgcgcgac cgagacagcc gcggcgttcg
     1081 gcatcgaatg tcatgcgggg catggtcttg attacgacac cgtgcggcaa atctcggcac
     1141 tcccgcgggt cgtcgagctc aacatcggcc atttcctcat cgccgatgcg atcttcatcg
     1201 gcttgggacc cgcgatccat aagatgcacg ccgcgatgac ggcggggcgg gccggggcga
     1261 tcgttttatg attgtgggtt tcgggatcga tctttgcgat atccgccgcg ttgaagagat
     1321 tttggagaag cacggcaagc gcttcgtcga gcgctgtttc accgaggtcg agcgcggcaa
     1381 atcggatcgg cgtgcggcgc gggccgcatc ttatgcgaaa agatttgccg ccaaggaggc
     1441 gtgcgccaag gccctgggga cggggctgac ccacggcgtc ttttggcgcg atatgggcgt
     1501 ggtcaatctg ccatcaggca agccgaccct gcatttgacc ggcggtgccg cggcgcgcct
     1561 cgcaattctt ctcccggccg gtcacatggc tgttatccat ttgactttga ctgatgatta
     1621 tccgctggct caggctcagg tcatcatcga ggcggtgaaa agttcggggc cgaacacgga
     1681 accgccgtga ttgcccgtca agcgatggcg aaggccggtg ctatcgcccg gcgtttttca
     1741 tgacgaaacc gacacttccc gctgtcacgg gcgcatgcta aggaccccgg cttccgagag
     1801 catgcgcttt cgagtggaaa gaaacggccg atgacgacga cggatcaaaa aggctacggt
     1861 gggatggagc agggcaagaa agccggggct ttcggcgagt ttttcaaggt catcattcag
     1921 gcgggcctta tcgcgcttgt cgtccgcacc ctc
//
LOCUS       DASWOG010000681         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_181922,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000681 DASWOG010000000
VERSION     DASWOG010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181922"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="queE"
                     /locus_tag="VGB93_13990"
     CDS             complement(<1..456)
                     /gene="queE"
                     /locus_tag="VGB93_13990"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY0147227.1"
                     /translation="MTYAVKEIYYTLQGEGAQAGRAAVFCRFAGCNLWSGREADRAEA
                     QCRFCDTDFVGTDGPGGGKFADATALATAIKAKWPGETSNALVIFTGGEPFLQLDEAL
                     ITAVHARGFTCAVETNGTLAPPPGLDWICVSPKADAPIALKSGDELKLVF"
     gene            complement(593..913)
                     /gene="trxA"
                     /locus_tag="VGB93_13995"
     CDS             complement(593..913)
                     /gene="trxA"
                     /locus_tag="VGB93_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0147228.1"
                     /translation="MATVKVTDATFKTEVEGASEPVVVDFWAEWCGPCKMIGPSLEEI
                     AEEMKGQVKIVKLNVDENPGVAGAFGIRSIPTLLLFKDGKLAQQKVGAAPKGELKKWI
                     AAAI"
     gene            complement(1017..4451)
                     /gene="addA"
                     /locus_tag="VGB93_14000"
     CDS             complement(1017..4451)
                     /gene="addA"
                     /locus_tag="VGB93_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEY0147229.1"
                     /translation="MRPIPPHVTALQRKASDPAASVWVSANAGSGKTHVLAQRVLRLL
                     LDGVPPAKILCLTFTKAAAANMAERVFKALSTWTAFDDVGLAKALAEIGAPKIDAATL
                     IFARRLFARTVETPGGLKIHTIHGFCERLLHLFPFEANVPGCFEVLDDLGEAELLVLA
                     RREALMQAERDRGALGAALKTLATETSEDDFNGLLKEAMKYRELLRHHDTNRARRHLA
                     SALGVEDGTSVATIETEMIEGGIGPARWREFAEFLRTGLPTDQEKADLLLAAMAAHQH
                     SQLSDCLEAYLGIFFTQKGEPRKTLVTKTPAGMRPDLADALLQERERLDRLRALRKAA
                     ACLEKSVALTTLAAAIFARYEAAKAQRGKLDFDDLIDRTLALFERSDARWVLYKLDSG
                     IDHILVDEAQDTSPAQWRILEHLASEFFAGAGSHSQTRTFFAVGDEKQSIFSFQGAAP
                     DMFHAMRANLAKKFTDAAQTFEHVSLKTSFRSVPAVLDAIDRVFTAEAHQRGLVSADV
                     WMGHESLKQSLPGLIEIWQPVTAPERPDSEDWRLPLDLLDESDPANVLAGRVAKKIAA
                     LIAPGSSERIYDTKLEHFRPITAGDVLILVRTRNALFDGMIRALKQAHVPVAGADRLD
                     LLAHIAVMDLIAAGHAALLPQDDLTLAAVLKSPLIGFDDDDLLRLAPNRRGSLFAALG
                     HSSEAKDKAVYGTIARWRARTAGTAFDFYARLLSEDGGRRAMEARLGPEACDAMDEFL
                     RLALRAEKDGTRSLLAFLAEIESLELSIKRDMESAADAVRVMTVHAAKGLEAKIVFLP
                     DTCSAPSGRHDAKIHLLPVVESGGKILAWSPRKDDDPDAVVQARQAARDEAENEHRRL
                     LYVAMTRAEERLYVSGFYRKNEPSALCWGAMIEATRGDAFVEMPAFWGEDETIWRYQA
                     PGEDGPFADAATASPRTEAPLPAWLLTCAPVEASPAPPLRPSSALAAGEPEMPPPSAE
                     RHAALARGRLVHTLLQYLPDVEAARRRSAAEVFLRARAPDFDDEARSALIGQVLGVLD
                     MPALAALFGSSVRAEVGLAGTAPLPNGKTREILGQIDRVAESEAEVIVADYKTGTPVA
                     ADATPENYLTQMALYRAVLAPLWPHKNLRMLLIWTEGPTIVELSALQLDEALAGIA"
     gene            complement(4448..>4750)
                     /locus_tag="VGB93_14005"
     CDS             complement(4448..>4750)
                     /locus_tag="VGB93_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147230.1"
                     /translation="MARRGAFGLAEGTDVAEAIYVKLYARDGGDERALKFKTPDAQLI
                     EISEKHFAELVALLNQFRDPATGYPARPYPKFAARYNAYDHLARVKEWAAGREDGA"
BASE COUNT          888 a         1498 c         1471 g          893 t
ORIGIN      
        1 gaaaaccagc ttcaattcgt cgccggattt gagtgcgatt ggcgcgtctg ccttgggact
       61 cacgcaaatc cagtcgaggc ccggcggtgg cgcgagcgtg ccattggtct cgaccgcgca
      121 ggtgaaaccc cttgcatgca cggccgtgat taaagcttcg tcgagctgca gaaacggctc
      181 gccgccggtg aatatgacaa gcgcatttga cgtctcgccc ggccatttgg ccttgatggc
      241 tgtggccagc gctgttgcgt ccgcaaactt gccaccgccg gggccatcgg tgccgacaaa
      301 atccgtatcg cagaagcggc attgcgcttc ggcgcggtcg gcctcgcggc cggaccagag
      361 attgcagccg gcaaagcggc agaagacggc ggcacggccc gcctgcgcgc cttcgccttg
      421 cagcgtgtaa tagatttcct tgaccgcata ggtcatcggc aacaccgggc tcgttttggc
      481 gcgttttctt tacgcgaacc ggcatccact tcgctcgaaa acactatgag tccgcaagca
      541 aaaaggacct cttgcgaggt cctttggcta ggccggaaaa caatcagcgg gatcagatgg
      601 ccgcggcaat ccatttcttc aactcgccct tcggcgcggc gccaaccttc tgctgcgcaa
      661 gcttgccgtc cttgaagaga agcagggtcg gaatgctacg gatgccgaag gcaccggcga
      721 cgcccgggtt ctcgtcgaca ttgagcttca cgatcttgac ctggcccttc atttcctcgg
      781 cgatttcctc aagcgaggga ccaatcatct tacaagggcc gcaccattcc gcccagaaat
      841 cgaccacgac gggctcggat gcgccctcga cctcggtttt gaacgtggca tcggtaactt
      901 taacggtggc cataggggcg cctcttgaac gctggctgcg agaccaaatt cgcgcagttt
      961 tcgttggtta aacgtaggaa gcagggcccg agccgtcaag ccgagacgcc ctttcgtcag
     1021 gcaatgcctg ccaatgcctc atccagttgc agtgccgaga gttcgacgat ggttggccct
     1081 tccgtccaaa tcagcagcat tcgcaagttc ttgtgcggcc agaggggggc gagcacggcg
     1141 cgatagagcg ccatctgcgt gagatagttt tccggcgtcg catcggccgc gacgggcgtg
     1201 ccggtcttgt agtccgcgac gatcacctcc gcctccgatt cggcaacccg gtcgatctga
     1261 cccaaaattt cccgggtctt tccgttgggc agtggcgccg tcccggcaag cccgacctcg
     1321 gcgcgcacgg aggacccgaa cagcgcggca agcgccggca tgtcgagcac gcccaaaacc
     1381 tggccgatca gggcgctgcg cgcctcgtcg tcgaagtccg gcgcccgcgc ccgcaagaaa
     1441 acctccgctg ccgaccgccg ccgcgccgct tcgacatccg gcagatattg cagcagcgta
     1501 tgcacgaggc ggccgcgcgc cagcgccgcg tgacgttcgg cgctcggcgg cggcatctcg
     1561 ggttcgcccg ccgcgagagc gctcgacgga cgtaacggtg gcgcgggcga ggcctcaacc
     1621 ggtgcgcacg tcagtagcca cgcgggcagc ggcgcttctg ttcgcgggga tgcggttgca
     1681 gcgtcggcaa aaggcccgtc ttcgcccggt gcctgatagc gccagatggt ttcgtcctcg
     1741 ccccagaagg ccggcatttc gacaaaggcg tcaccgcgcg tcgcttcgat catcgctccc
     1801 caacagagcg cgctcggctc gttcttgcga tagaagccgc tgacatagag ccgctcctcg
     1861 gctcgcgtca tcgccacata aagcaggcgg cgatgttcgt tctcggcttc gtcgcgcgcc
     1921 gcctgccgcg cctgaacaac ggcgtccgga tcgtcatcct tgcgcggcga ccaggcgaga
     1981 attttgccgc cggattcgac aacgggaagc agatggattt tcgcgtcatg ccggccgctc
     2041 ggcgcgctgc aggtgtccgg caaaaagacg atcttggcct caagcccctt agcggcatga
     2101 acagtcatca cccgcaccgc gtcagcggcg gattccatgt cgcgcttgat cgaaagttcg
     2161 aggctttcga tttcggcgag gaaggccagc agcgaccgcg tgccatcctt ttcggcgcgc
     2221 agagcgagac gcagaaattc atccatggca tcgcaggctt ccgggccaag ccgagcttcc
     2281 atcgcgcggc gcccgccatc ttcggagagc agacgcgcgt agaagtcgaa agcggtcccc
     2341 gctgtccgcg cgcgccaacg cgcaatcgtt ccatagacgg ctttatcctt ggcctcgctg
     2401 gaatgaccaa gcgccgcgaa gagcgagcct cttcggttcg gcgcgaggcg caacaggtcg
     2461 tcatcgtcga agccgatgag cggcgacttc aggacggcag cgagcgtaag gtcgtcctgt
     2521 gggagcaagg ctgcgtgtcc ggcagcgatc aggtccatca ccgcaatatg ggcgaggaga
     2581 tcgaggcggt cggcaccggc caccggcacg tgcgcctgtt tcaaagcgcg aatcatgccg
     2641 tcgaagagcg cattgcgcgt gcgcacaaga atcagcacgt caccggcggt aatgggacgg
     2701 aagtgctcca gcttcgtatc atagatacgc tcgctggagc ccggcgcgat cagagcggcg
     2761 atctttttcg caacgcggcc ggcgaggacg ttcgccggat cactctcgtc aagaagatcg
     2821 agcggcaggc gccaatcttc ggagtcgggc cgctctggcg ccgtcaccgg ctgccagatt
     2881 tcgatcagac cgggcaatga ttgtttcagc gattcatgcc ccatccacac gtcggccgaa
     2941 acgagaccgc gctgatgcgc ctcggccgta aacacgcgat cgatggcatc gagtaccgcc
     3001 ggcacggagc ggaacgaggt tttcagcgac acatgctcga atgtctgcgc cgcgtcggtg
     3061 aatttcttcg ccaggtttgc gcgcatggcg tggaacatgt ctggcgcggc gccttgaaac
     3121 gaaaaaatcg actgcttctc gtcgccgacc gcgaagaaag tgcgtgtctg gctgtggctg
     3181 ccggcaccgg cgaagaattc gctcgccaga tgttcgagaa tccgccattg cgccgggctc
     3241 gtatcctgcg cttcgtcaac gagaatatga tcgatgccgg aatcgagctt gtagagcacc
     3301 cagcgcgcat cggagcgctc gaagagcgcg agcgtgcgat cgatgagatc gtcgaaatcc
     3361 aattttccgc gctgcgcttt cgcagcttcg taacgggcga agatcgctgc ggcgagtgtc
     3421 gtcagcgcga ccgacttttc gagacatgcg gcggctttgc gcagcgcgcg caggcggtcc
     3481 agtctttccc gctcttgtaa aagtgcatcg gcgagatccg ggcgcatgcc ggcgggcgtc
     3541 ttcgtcacca gtgtttttcg cggctctcct ttttgcgtga agaaaattcc caaataagct
     3601 tcaaggcaat ccgagagttg gctgtgctga tgcgcggcca tcgccgcaag caaaaggtca
     3661 gctttctctt ggtcggtcgg aagacctgta cgaagaaatt ccgcgaattc gcgccaacgc
     3721 gctgggccga ttccgccctc gatcatttcg gtttcgatcg tcgcgacact cgtgccgtcc
     3781 tcgacgccga gtgcactggc aagatggcgc cttgcgcgat tggtatcgtg atgccgcaat
     3841 agttcgcgat atttcatcgc ttccttgagc aggccattga aatcgtcttc cgaagtttcc
     3901 gtggcgagcg tcttcaaggc cgcgccaagc gcgcctctat ctcgttcggc ctgcatcaag
     3961 gcctcgcgcc gcgccaatac gagcaattcg gcttcgccaa gatcatcaag cacctcaaaa
     4021 caccccggca cattggcctc gaaggggaag aggtgcagca gccgttcgca aaagccgtgg
     4081 atcgtatgaa tcttcaggcc gcccggcgtc tcgacggtgc gcgcgaaaag acgacgcgcg
     4141 aaaatcaatg tggcggcatc gatcttcggc gcgccgatct cggcgagcgc cttggcgagc
     4201 cctacatcgt cgaaagccgt ccaggtggag agcgccttga agacgcgctc ggccatattg
     4261 gcggcggccg ccttggtaaa ggtgagacac aaaattttcg ccggcggcac gccgtcgagc
     4321 aaaagccgca gcacgcgttg cgccagcaca tgcgtcttgc ctgatccggc attggccgag
     4381 acccagacgg aggcggccgg atcggacgct ttccgctgca aagcggtgac gtgcggcggg
     4441 atcggtctca cgcgccgtcc tcgcggcccg cggcccattc ttttacgcgc gccagatggt
     4501 cgtaggcgtt gtagcgtgcg gcgaatttcg gatagggccg cgccggatag cctgtcgccg
     4561 gatcgcgaaa ctgattgagc agtgcaacga gttcggcgaa atgcttttcc gaaatttcaa
     4621 tgagctgcgc gtccggggtt ttgaatttta gcgcccgctc gtcgccaccg tcacgcgcgt
     4681 agagcttcac atagatcgcc tcggcgacat ccgtcccttc cgcgagtccg aaagcgccgc
     4741 ggcgagccat
//
LOCUS       DASWOG010000682         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_329255,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000682 DASWOG010000000
VERSION     DASWOG010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329255"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGB93_14010"
     CDS             <1..192
                     /locus_tag="VGB93_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147231.1"
                     /translation="PPAIIVWGKNDFIFPEPGAHPYKRDLRDLEFHLLDTGHFVLEGK
                     ADEAFPLIRDFLDRKVASA"
     gene            225..503
                     /locus_tag="VGB93_14015"
     CDS             225..503
                     /locus_tag="VGB93_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006699205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HEY0147232.1"
                     /translation="MKDYIAALTAGALVGIIYGLINVRSPAPPVIGLVGLLGILLGEQ
                     AVPLAKRLISAQPVTISWLRAECEPHVLGKLPVGRTSTLESGLKEAER"
     gene            640..1746
                     /locus_tag="VGB93_14020"
     CDS             640..1746
                     /locus_tag="VGB93_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0147233.1"
                     /translation="MRRMRNWLASVAFVAPVLSLTAAYAQDVSNPAAPYVPKACSTHV
                     TVAPADVADDPNLDPAVRKFLIPLDKDPSPFWEQPQPKPQQVVTDLQNQTPVDMSGVT
                     ISEQSLTVDGRTIKLFIVKPDHLEGHPGVILFIHGAVWLAGNFENHKRLVRDLVVESG
                     QPAVFPEITNLPVGKYPVPLNQAYATLEWIAAHASEFGADGSRIAVAGNSVGGNMTAA
                     LNLMVKDRKGPKISYQVLLWPATNAGVDTCSYEEYANGRFLSRAFMKYGWDRYAPTEK
                     ERENPYVSPLRASTTELQGLPPTLVITEENDVLRDEGQAYAHRLQDAGVPTVSVRYNG
                     TIHDFGLLNALVDLPTTQAMIHQVAGSIRKHIGE"
     gene            complement(2323..2628)
                     /locus_tag="VGB93_14025"
     CDS             complement(2323..2628)
                     /locus_tag="VGB93_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147234.1"
                     /translation="MHDGPVKILLQTTIHPTEDDWSIARFSRLGALLSAAKTPSRDAM
                     YEVTMRDRDPLGIPGSVLSKLDQSDYDELWLFAVDVGDGLTESDCEAILRFRHRGRG"
     gene            2820..3788
                     /locus_tag="VGB93_14030"
     CDS             2820..3788
                     /locus_tag="VGB93_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY0147235.1"
                     /translation="MRGMTAKVFGGYADLKLTDLPKPSAGEDRVLVRITAAGVTPLDH
                     TILSGQFPLANPPLVLGNEGAGVVEAGDDEIPIGTRVMFTGPYGVFENGTYSDYVAVK
                     KEHLCVIPAGISDTTAAGLPIAYLTAYLALSDAGFEAGKSVLAPAIGGSVGNAATQLA
                     RAMGAEHAISTTTNPAKAKQAQELGFSEIVDLSKEGLADGIRRLTDGYGVDIVIDGIG
                     GEVLSEALGALAQGGSLTTLGYSAGRKSTIDVTNLIWKGASIRSFLLFSKSPADWAKA
                     WTTIIPLLASGQVVPVIAKTFSLEEAPEALRYLVEERPFGRVILTL"
     gene            complement(3848..4288)
                     /locus_tag="VGB93_14035"
     CDS             complement(3848..4288)
                     /locus_tag="VGB93_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0147236.1"
                     /translation="MARKAPVPDDFICFAVYSTSHAFNQVYKPLLKILGVTYPQYLVL
                     VSLWAKDDQTVGSLGNLLFLESNTLTPLLKRLEGLDLVMRNRDPDDERQVRVQLTKKG
                     DTLRDKARDFPSCVDGATGLAPDALRQLREAIILVRATLLKEAG"
BASE COUNT          961 a         1319 c         1319 g          930 t
ORIGIN      
        1 cctccagcga ttatcgtttg gggcaaaaac gatttcattt tccccgagcc tggcgcgcat
       61 ccctacaagc gtgatctgcg ggatctcgag tttcacctgc tcgatacggg ccactttgtg
      121 cttgaaggca aagccgacga ggcctttccg cttattcgtg actttctcga tcgcaaggtg
      181 gccagcgctt agcgtcaacc gtagcaaact gtaaagagat atccttgaaa gactacatcg
      241 ctgccctcac cgcgggggcg ttggtcggaa ttatctatgg tctcatcaac gtccgttcgc
      301 cagcgcctcc ggtcatcggt ctcgtgggct tgctagggat tctacttggt gagcaagctg
      361 tccctctggc gaaaagactg ataagcgcgc agcccgttac aatctcgtgg ttaagggcag
      421 aatgcgagcc gcacgtactt gggaaactgc cggtcgggcg cacttcgacg cttgaatcag
      481 ggctcaagga agcggagcgc tgacgtgcga ttatgtaaac aggtatggtg atttggaaga
      541 gcgcgcgacg aatatgcgct aaccgggtgc atcgctagat cgaagcttcg tgcggtctga
      601 tcaggcccgg attcgatcgc gatcacttgg agatcgatta tgcgtagaat gagaaattgg
      661 ttggcgagcg tcgccttcgt ggcgcctgtc ctaagtctca cggcagctta tgcccaggat
      721 gtaagtaatc ccgctgcgcc ttatgtgccg aaagcttgct cgacgcatgt caccgtcgcc
      781 cccgccgatg tcgccgatga tcccaacctt gatcctgcag tccgcaaatt tctgatcccg
      841 ctcgacaagg accccagccc gttttgggaa cagcctcagc ccaaaccaca gcaggttgta
      901 accgatctgc agaaccagac tccggtcgac atgtccggcg taacgatctc ggagcaatcg
      961 ctcaccgtcg atggccgcac gataaagctg tttatcgtga agcccgatca cctcgaaggg
     1021 catccgggcg tcattctctt tatccatggc gccgtttggc ttgcgggtaa cttcgaaaac
     1081 cataagcggc ttgtgcgcga tctcgtcgtc gaatccgggc agccggccgt gtttcccgaa
     1141 attactaatc tcccagtcgg gaagtatccg gtaccgttga accaagctta cgcgacactt
     1201 gagtggatag ccgcgcatgc atcggaattc ggcgcagatg gaagccggat cgcggtggcc
     1261 ggaaactcag tcggcggcaa catgaccgcg gccctgaacc tgatggtcaa ggatcggaag
     1321 gggccgaaga tcagctatca ggtcctgctc tggccggcga ccaatgccgg cgtcgatacg
     1381 tgctcctatg aagaatatgc caatggccgc ttcctgagcc gcgcgtttat gaagtatggc
     1441 tgggatcgtt atgcgccgac cgagaaagaa cgggaaaacc cgtacgtttc gcctctgcgc
     1501 gccagcacga ctgaattgca aggcctcccg ccgaccttgg tcatcaccga agaaaacgat
     1561 gtgctacggg atgagggcca agcctatgcg catcggctgc aagatgccgg cgtgccgacg
     1621 gtctccgttc gctacaacgg aacaatccat gactttggct tgctgaatgc gctcgtcgat
     1681 ctgccgacga cccaagcgat gatccatcag gtcgcgggaa gcattcgcaa gcatatcgga
     1741 gagtaagacg gtctctgatc agccgcggcg ggcgagccaa gccgcggcgt tcagcatata
     1801 gcgacgtaga agcgacggcc gcccgcgtcc gggcaaggga atcgcccggc ggttcgtcga
     1861 caaagcttgg gcatccggaa gaggtgtccc agttgtagtc ggcgaaatga tgaaacgtgc
     1921 tttgagcgat cgcgcgaccc gatccgttcg acgcctcgaa ggcgaccgcc agattgaaca
     1981 cgcggccgga tatttggctt ttgcctctgg cgatcacgcg cgcgtcttca ccgtctggtg
     2041 ccgcgatcgc cccttcgtgc ggatgggtgg gcaaaaaccg cacatagccg tcaggcgcct
     2101 ctgaatcggt caatatggga tgcagcggca acaccggctc tacgatttga aaatcgccgt
     2161 tcgcaccgga atgataattc ggccagctga tattggtcga atagggatcg tcaatccgac
     2221 agttttccgc gatcggatcg acattgcgcg aatggaaatg atgcgccttt ccaacgcccg
     2281 ccaacccaca gatcgaagaa cccaagtcca tatgatcgcg cgtcagccgc ggccccggtg
     2341 ccggaacctt aagatcgctt cgcaatccga ctccgtaagc ccatcgccga cgtcgacggc
     2401 aaagagccat agttcgtcat agtcgctttg atcgagcttg gagagaacgc tgccggggat
     2461 gccgagcgga tcgcggtcac gcatcgtcac ctcgtacatc gcatcgcgcg atggcgtctt
     2521 cgcggcgctc agcaacgcgc caagacggct gaagcgtgcg atcgaccaat cgtcctccgt
     2581 tggatgaatc gttgtctgca gaagaatttt gactggaccg tcatgcatga caagctttct
     2641 gcctcgggct tcggtgacgc cgtcgtgact ttcgcacaga tgctaaatcg actccatcag
     2701 cctgcggcat gatcgcgtct ataccaatgg ataggatcac gcggcagcga tgttgcgttc
     2761 tggttggggc gaatggtgcg gtcgcgtccg catctccgaa gaatgccagg agggccaaga
     2821 tgcgcgggat gacggcgaaa gtctttggcg gctatgccga cttgaaattg accgaccttc
     2881 ctaagccaag cgccggcgaa gaccgcgttc tggtgcgcat caccgcggcg ggcgtaacgc
     2941 cgctcgacca cacaatcctg tcgggtcaat ttccacttgc caatccgccg ctcgtgctcg
     3001 gcaacgaggg tgccggcgtg gtcgaggcgg gcgacgatga gatccccatt ggcacccgcg
     3061 tcatgttcac cgggccgtat ggcgtcttcg agaatggcac ctacagcgac tatgtcgccg
     3121 tcaagaagga gcatctctgc gtgatacccg caggaatatc agacacaacg gcggcgggcc
     3181 ttcccattgc ctatctgacc gcctatctcg cgctcagcga tgccggtttt gaagcgggta
     3241 aatccgtatt agctcccgcg attggcggct ccgtcggcaa tgcggcgacg caattggcgc
     3301 gggccatggg cgccgagcat gccatctcca cgacgaccaa tccggcgaag gcgaagcagg
     3361 cacaggaatt gggctttagc gaaatcgtcg atctcagcaa ggagggcctg gctgatggta
     3421 ttcgccggct gaccgacggc tatggcgtcg acattgtcat cgacggcata ggcggcgagg
     3481 ttctgagcga ggcgctcggt gcgttggccc aaggcggcag cctgacgacg ctcggctatt
     3541 cggccggccg caaatcgacc atcgacgtca ccaatctcat ctggaaaggc gcgagcatca
     3601 gaagctttct gctcttttcc aaatcgccgg cggattgggc gaaagcctgg acgactatca
     3661 ttccgctttt ggcgagcggc caagtcgtgc ccgtcatcgc caagaccttt tcgctcgaag
     3721 aggcgccgga ggccctccgt tatctcgtcg aagagcgccc ctttggccgc gtgatcctga
     3781 cgctctaaag tagtccctgc ggtcgctcgc gacttgagcg tatcgccagc tcttcctctg
     3841 cgcgaattca gccggcctct ttgagcaatg tcgcgcgcac caagatgatc gcctcgcgca
     3901 attgccgaag cgcgtccggc gcgaggcccg tcgcaccatc gacgcaggac gggaaatccc
     3961 gcgccttgtc gcgcaatgta tcgcccttct tcgtcaactg gacacgcacc tggcgctcgt
     4021 cgtcgggatc gcggttacgc atcaccaaat cgagaccttc gagccgtttg agcaatggcg
     4081 tcagcgtatt ggattcaagg aaaagcagat taccaaggct gccgacggtc tgatcgtcct
     4141 tggcccagag cgacacgaga acgagatatt gcggataggt gacgcccaat attttgagca
     4201 gcggcttata gacctgattg aacgcgtggc tggtcgaata gaccgcaaaa cagatgaaat
     4261 cgtcaggcac tggggctttg cgggccataa atcatccttc tgcgcatgaa tgcggccagt
     4321 tggcgcatcc ctagataaat cgcacgcgat tagatcgcaa gatattgacc gaggcggaag
     4381 cagcacctaa tttgatcgta tacgatctaa tcgtttacga taataatcgt aaactgtcgg
     4441 cctgaggtaa gcccgatgaa tctgcgtgcc gacggaaccc gtaaatatcc aaattcgaaa
     4501 ttgatcgctt catccgccga ttgcggctg
//
LOCUS       DASWOG010000683         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_291458,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000683 DASWOG010000000
VERSION     DASWOG010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291458"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1453)
                     /gene="hpnE"
                     /locus_tag="VGB93_14040"
     CDS             complement(191..1453)
                     /gene="hpnE"
                     /locus_tag="VGB93_14040"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY0147237.1"
                     /translation="MSRIHIVGAGLAGLAAALQLADGKRSITIHESATQAGGRCRSYF
                     DATLGMEIDNGNHLLLSGNYAAQQFLRTVGSEDQLVGPEHAEFAFVDLKTRERWQLQP
                     SDGAIPWWIFQKDRRVPGTGPLDYLSVAPLLTAAKGATLNQAMKCEGVLWERLWQPFF
                     LAALNTQPEEGAASLAARLMRETFAKGGRACRPLVAAQGLSTAFIAPALRHLETKGVG
                     IRFERRLSAIAADGGNVKALSFGDETIALEHGDALVLAVPAPVAASLLPALPTPQSFR
                     AIVNAHYKIASPPGQPPILGIINGTMEWLFAFPGRLSVTISGADRLIATPREELAAKI
                     WSEIQLVTKIDSPLPAWQIIKEKRATFAATPAEEVRRPKTRGAWPNLLLAGDWTDTGL
                     PATIEGAIRSGFAAADAALADEWRRCPI"
BASE COUNT          318 a          486 c          493 g          287 t
ORIGIN      
        1 gcgcacacgg cggcgttccg gaaggaatgc gtgtcatccg tatggaacgc gccgaaaccg
       61 ccgttcttgc tttgcctacc cgcgatccaa tcgaggctaa acaattttag cttccgataa
      121 tagtcacaga cgtctcgaaa agcgctgccg cccgatcgcg tactgggcag ggtggccccc
      181 tcatccccag ctaaatcggg caacgcctcc attcgtcagc caaggcggca tcggccgcag
      241 cgaaacctga gcgaatggcg ccttcgattg tcgccggtag ccccgtatcc gtccaatctc
      301 cagccaggag gagattgggc cacgcaccgc gggtcttagg ccgccgcacc tcttctgccg
      361 gtgtcgcggc aaaggtggcg cgtttctctt ttatgatctg ccatgcgggg agaggcgagt
      421 cgatctttgt aaccaattga atttccgacc atattttggc ggcgagttct tcccgtggtg
      481 tcgcgatcaa acggtccgcg ccgctgatcg tgaccgacag ccggccgggg aaggcgaaga
      541 gccattccat cgtcccgttg atgatgccga ggatcggcgg ctggcccggc ggggatgcga
      601 ttttgtaatg tgcgttcacg atggcgcgaa aagactgcgg cgtaggcaaa gctggcaaaa
      661 gcgaggcagc aaccggtgcc gggactgcca gaacgagagc atcgccatgc tcaagggcaa
      721 tcgtttcgtc gccaaaggag agggctttga cgttaccccc gtccgcggcg atggccgaca
      781 gccgccgctc gaagcgaatg ccgacgccct ttgtttcaag atgccgcagc gcgggagcga
      841 tgaaggctgt cgaaagcccc tgcgccgcca ccagcgggcg acaggcgcgg ccgcccttgg
      901 cgaaggtttc ccgcatgagc cgggcggcga gcgaggctgc gccttcctca ggctgggtgt
      961 taagtgccgc gaggaaaaac ggctgccaaa gccgctccca aagcacaccc tcgcatttca
     1021 tcgcctgatt cagcgtcgcc cctttcgccg cggttagcag cggcgcgacg ctgagatagt
     1081 cgagcggccc agtgccgggc acacggcgat ctttctgaaa aatccaccac ggaatggcgc
     1141 cgtcgcttgg ctgcaactgc cagcgctcgc gggtcttcag atcgacgaaa gcgaattccg
     1201 catgttcggg gccgacaagc tgatcctcgc tgccgactgt gcgcaaaaac tgctgcgccg
     1261 cgtaattgcc ggagagcaac agatggttgc cattgtcgat ctccatcccg agcgtcgcat
     1321 cgaaatagga gcggcagcgg ccgccagcct gcgtcgcgga ctcatgaatg gtaatagatc
     1381 gctttccatc cgccagttgc aacgcagcgg caaggccggc cagtccggcg ccgacgatat
     1441 gaatgcgcga catcagaaca gaccgtggcg cagcacgatg agcactagtt ggtgcttggg
     1501 cagcttgatc ttctggcgcg gcggagcgaa gccgcgtgcc aataaagtgc cgagaatgat
     1561 ctgataggct tcatacatca gccg
//
LOCUS       DASWOG010000684         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_181944,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000684 DASWOG010000000
VERSION     DASWOG010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181944"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1654
                     /locus_tag="VGB93_14045"
     CDS             167..1654
                     /locus_tag="VGB93_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0147238.1"
                     /translation="MLHRLPRIGSRLSFLACLSASLAGCMMGPDYVRPAAPIAPHFKE
                     AKKGWKLADPADALDRGEWWAVYHDPKLASLMSQVEISNQTVKQALEAYHNSVALIRE
                     AQANYFPTVTNSYSATYQHQGSGTFTSGALAARSSTFTTFNPVANASWDLDVWGRIGR
                     QVESQAAAAQVSAADLQNAKLSEQAALATAYFDMREEDSLKALLDRTIVAFKKTLTIT
                     QNQFKAGTVSQGDVITAQTQLLQTEAQAIDTGVGRAQFEHSIAILIGRPPSDLALSPA
                     ELAPYPPHIPATVPSLLLERRPDIAAAERQMQEENALIGVAVANFYPDINLSGAFGFA
                     GATPLPISAAAEAWSIAGTATQTIFDGGLLAADLAAARATYAQNIASYRQTVLTAFDQ
                     VEDELAALRILAKEAAKQVETVKAARQAVQIDLNQYQAGTVPFTTVVTGEATQLQDEE
                     NLLTTRQNLFIASVALIEALGGGWDASQLPSLARLSKVPTFTPPL"
BASE COUNT          386 a          586 c          552 g          376 t
ORIGIN      
        1 tgtctgctag cttgatcgtc tcggtttaag cggcggcgct gatccaggac tcatcttgca
       61 agcctgttat attgggtgcg aaggaggaac gctgccgcag gctctggccg aacagcattt
      121 ttctcgccgg cacggcatca atgagtttcg acctttggat tggcttttgc tccatcgcct
      181 gcctcggatc ggttcgagac tgtcgtttct cgcgtgccta agtgcgagtc tcgccggctg
      241 catgatgggg cccgattacg tgcggcccgc ggcgcccatc gcgccgcatt tcaaggaggc
      301 caagaaaggc tggaaacttg ctgaccccgc cgatgcgctg gatcgcggcg aatggtgggc
      361 ggtctatcac gatcccaaac ttgcatcgct gatgagccag gttgaaatct ccaaccagac
      421 ggtcaagcaa gcgcttgagg cctatcataa ttctgttgcc ctgatccgcg aggcgcaggc
      481 caattatttc ccgacggtca ccaatagcta cagcgctaca taccagcatc agggcagcgg
      541 aacatttaca agtggggctc ttgctgcgcg cagttccaca ttcacgacct tcaatcccgt
      601 cgccaacgcg tcatgggatt tggatgtgtg gggccgcatt ggccgccaag tcgagagcca
      661 ggcggccgcg gcccaggtca gcgccgccga tctgcaaaac gccaagctct ctgaacaggc
      721 cgcccttgcc acagcctatt tcgacatgcg cgaggaggat tcgctcaaag cgctgctcga
      781 tcgcacaata gtcgccttca agaaaacgct gacgattacc caaaatcagt tcaaagccgg
      841 cactgtctcg cagggcgacg tcatcaccgc gcaaacccag cttttgcaga ctgaggcgca
      901 ggccatcgat accggcgtag gacgcgcaca attcgagcat tcgatcgcga ttctgatcgg
      961 ccggccgccg tccgatcttg cgctcagccc ggctgaactg gcgccctatc cgccgcatat
     1021 tcccgcgacc gtaccctcgc tgctcctgga acgtcgcccg gacatcgccg cggcggaacg
     1081 acaaatgcag gaagaaaatg ctctgatcgg cgtcgcggtg gcgaatttct atcccgacat
     1141 caacctgtcc ggcgcctttg gcttcgccgg cgcgacgccg ctgccgatct ccgcggctgc
     1201 agaagcttgg tcgattgcgg gaaccgctac acaaacgatc ttcgacggtg ggctgctcgc
     1261 ggccgatctc gccgctgcgc gggcgactta cgcacaaaat atcgcgagct atcggcagac
     1321 ggttctgacc gccttcgacc aggtcgagga cgaattggcg gcgctgcgga tcttggcgaa
     1381 agaagcggcc aagcaggtag aaactgtgaa agcggcgcgc caggcggtgc agatcgacct
     1441 taatcaatac caggccggca ccgtcccctt caccactgtg gtcacagggg aagcgacgca
     1501 gctccaggac gaggaaaacc tgctgaccac ccggcaaaac ctgttcatcg ccagcgttgc
     1561 cctgatcgag gccttgggcg gcggctggga tgcgtctcaa ttgccaagcc tcgcgcgcct
     1621 ttccaaagtg ccgaccttca cgccgccgct ttaaaaatcc cgccagaagc ctgacttggg
     1681 cgtcaaaaat ctcaccaaga agtaatttgc ctttatcggc cctgatgagg aagggatgct
     1741 gacacgtcgc ggattattgc ccttatggga aaagaggcgc gaaccgggta ggttggtgat
     1801 gctttgccgg aactagtccc cctaggtaag gcttcgcaag gtggccgttc tgatctcggc
     1861 ggcgcctggt ttgcaagcca tagattaggg tccgcgcctc
//
LOCUS       DASWOG010000685         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_53203,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000685 DASWOG010000000
VERSION     DASWOG010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53203"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGB93_14050"
     CDS             complement(<1..481)
                     /locus_tag="VGB93_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0147239.1"
                     /translation="MISSEAGTTAEALRASPRLFDNPLLDKLSRVHWSIPLFIYTPVV
                     ALLAWLSFRNFSPFAVIFATAAGYAIWTLIEYFGHRYLFHAEFPGTWGARVHFLIHGV
                     HHDHPNDPLRLVMPPLLSGPIMLIALLVALVIFGRPLGYPILMGFIIGYVAYDMVHYY
                     "
     gene            481..624
                     /locus_tag="VGB93_14055"
     CDS             481..624
                     /locus_tag="VGB93_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147240.1"
                     /translation="MSNLLHRFDVIVHCTATTSWGHALVISLHDMDIWVFVKITAQSC
                     RKE"
     gene            682..>1777
                     /locus_tag="VGB93_14060"
     CDS             682..>1777
                     /locus_tag="VGB93_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147241.1"
                     /translation="MEVIVLDNGLQSQGEHSYRLLLEVRDALSRRKITHRIFGMRDAD
                     AAAIADIGAIPWFTKSLYWSGRQRLPSLQNFVSDSLSLMRSLGTRGYHYSEEATARVL
                     NKTYRRDLERLPNDVWTRDNLIVVPAVSQNQILGLVQHLLALPASALPTVVCQLMFAP
                     AWTPWNRNGCHGEDYYRQAFELAAPLIGKSLFFTTENDALAQLYLRVFKLKTGLLPIV
                     TRIPHAASQPEGTIKLGFFGYSKSEKGFHLLPEAIGLCRARNLPAQFVVQIQHSNWER
                     ETVETEKALRRIPDLEIIDGALDADEYTRQINEVDVTLLPYDPVRFGLRGSGIYTESV
                     AAGRPVVAAKGIYAALGIERGEAVGEIFAPY"
BASE COUNT          409 a          479 c          510 g          379 t
ORIGIN      
        1 cgtaataatg caccatatcg taggcgacat atccgatgat aaaacccatg agtatcgggt
       61 agccgagcgg acggccgaaa atcacgagcg ccacaaggag cgcgatcaac atgatcgggc
      121 cgctcagcaa tggtggcatc acaagtcgta acggatcatt gggatgatcg tgatgaacgc
      181 cgtggatcaa gaaatgcacc cgggcgcccc atgtgccggg aaactcggca tggaacagat
      241 agcgatggcc gaaatattcg atcaaagtcc aaattgcgta gccggccgcg gtggcgaaaa
      301 tgacggcgaa aggcgaaaag ttacgaaaac tcagccatgc caacaaagct acgacggggg
      361 tgtagatgaa cagcggtatt gaccaatgca cgcgcgaaag cttgtcgagc aaggggttgt
      421 cgaataggcg cggggaagcg cgcagcgctt cggcggttgt cccggcttca gaggatatca
      481 ttgagcaacc ttcttcatcg tttcgacgtc atcgtgcatt gtaccgcgac gacctcttgg
      541 gggcacgccc ttgtgattag cctccatgac atggatattt gggtgttcgt caagatcacg
      601 gcgcaatcgt gccgcaaaga gtagtttcaa cggcaaaatt atatgaatcc gccgaattcc
      661 gctacggggc aaaatactcg gatggaggtg atcgtcctcg ataacggtct tcaaagccag
      721 ggcgagcaca gctaccgcct gcttcttgag gtgcgggacg ctttgtcgcg ccggaaaata
      781 acccatcgca ttttcggtat gcgtgacgcg gacgcagccg ccattgcgga tatcggcgca
      841 attccctggt tcaccaagag cctttactgg agcggccgcc aaagattgcc gtcgctgcaa
      901 aatttcgtct ctgacagtct atcactgatg cggagtctgg gtacccgcgg ctaccattac
      961 agcgaggaag cgacggcacg cgttctcaac aaaacctatc gtcgcgatct cgagcgcttg
     1021 ccgaacgatg tctggacgcg ggataatctc atcgtcgtgc cggcggtctc gcaaaatcag
     1081 attcttggtc ttgtgcaaca tctgctggcg cttcctgcct cggcattgcc gacggtcgtt
     1141 tgccagttga tgtttgcacc ggcctggacg ccatggaatc gcaatggctg ccatggagaa
     1201 gactattata gacaggcctt cgaactcgcc gcgcccttga tcggcaagtc gttgttcttt
     1261 acgactgaaa acgacgcgct cgcgcagctt tatctgcggg tcttcaagct caagacaggg
     1321 cttctgccga tcgtcacccg gataccccat gcggcgtctc agccggaagg tacgataaag
     1381 ctagggtttt tcggttattc caaatccgag aagggatttc atctgcttcc ggaagctatc
     1441 gggctttgcc gcgccaggaa tttgccggcc caattcgtcg tccagatcca acattccaat
     1501 tgggagcgcg aaacggtcga gacagaaaaa gccctgcgcc ggattcccga tctcgaaatc
     1561 atcgatgggg cgctcgatgc tgatgaatat actcgacaga tcaacgaggt cgacgtgacg
     1621 cttttgcctt acgatccggt acgctttggt ctgcgcggct ccggcattta taccgaatct
     1681 gtcgccgcgg gccggccggt cgttgccgca aaaggaattt atgccgcgct cgggatcgaa
     1741 cggggcgagg cggtcgggga aattttcgcg ccctata
//
LOCUS       DASWOG010000686         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_329504,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000686 DASWOG010000000
VERSION     DASWOG010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329504"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VGB93_14065"
     CDS             <1..464
                     /locus_tag="VGB93_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0147242.1"
                     /translation="DITASRQAQDTLERAEAELAHVARIATLGELTTTIAHEVNQPLT
                     GIVTNGEACLRWLDRDEIDIDEVTDTLKRIVRDGRRAGDVIQRLRTLSQKAEPQRMPL
                     EINDIVTDAVSLLQREIDARHVSLKLDLLPHLPIVNAGSGHRNPRNGAHHR"
     gene            445..1074
                     /locus_tag="VGB93_14070"
     CDS             445..1074
                     /locus_tag="VGB93_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0147243.1"
                     /translation="MVLIIDDDISVREALDSLFRSVGLKTRAFVSAHEFLQSELPAMP
                     CCLVLDVRLPGISGLDFQTYLGETNVQLPIVFMTGHGDIPMSVRAIKAGARDFLTKPF
                     RDQDMLDAVTDAIALDRRRRQSEKDIAAPREVFETLTTRERDVMILLAKGLLNKQVAA
                     QLGLSEITVKIHRGRLMKKIGAYTLADIIKMADLLQLRDAPLSGPETGS"
     gene            1135..>1792
                     /locus_tag="VGB93_14075"
     CDS             1135..>1792
                     /locus_tag="VGB93_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0147244.1"
                     /translation="MSTLKLFVLPGDGIGPEVMTEVEKIANFLTKQGVAHFEIERGLV
                     GGAAYDAHQVAISEDDMARAGVADAILLAAVGGPKWDKVPYDVRPEAGLLRLRKDFNL
                     YANLRPAICYPALADASSLKRELIDGLDLLIVRELTGGVYFGEPKQVIDLGNGQKRAI
                     DTQVYDTYDIERTGRIAFELARNRRNKVTSSDTHNVMKSGVLWPEIIIDLHKREFPDV
                     E"
BASE COUNT          364 a          556 c          523 g          349 t
ORIGIN      
        1 ccgatataac ggcctcgcgg caggcacagg atactttgga acgcgctgag gccgagctgg
       61 ctcatgtcgc gcggatcgcg acgcttggcg aattgacgac gacaatcgcg catgaggtca
      121 atcagcctct gacgggaatc gtcaccaatg gcgaagcctg tctgcggtgg ctcgaccgcg
      181 acgaaatcga tatcgatgaa gttaccgata ctctgaagcg gatcgtgcgc gatggccggc
      241 gcgcggggga cgtgatccaa cgtttgcgga cgctttccca gaaggctgag ccgcagcgca
      301 tgccgctcga gatcaacgat attgtcaccg acgccgtctc gcttctgcaa cgcgagattg
      361 acgcgcggca tgtttcgctt aagctcgacc tgttgccgca tctgcccatc gtcaacgccg
      421 gaagcggaca ccgcaatcca cggaatggtg ctcatcatcg atgatgacat ttccgtgcgc
      481 gaagcactcg acagcctgtt tcgctccgtt gggctcaaga cgcgcgcctt cgtctcggcg
      541 catgaattct tgcaaagcga gctaccggcg atgccgtgct gtctcgtgct cgacgtccgg
      601 ttgccgggga tcagcgggct cgattttcag acttatcttg gcgagaccaa tgtccagctt
      661 ccgattgtct tcatgaccgg ccacggcgat attccgatgt cggtcagggc tatcaaggct
      721 ggcgcgcgcg attttctgac gaagccattt cgcgatcagg acatgcttga cgctgtcacg
      781 gacgcaattg cgctcgaccg ccgccgccgc cagagtgaaa aagacatcgc ggccccccgc
      841 gaagttttcg agacgctgac gacgcgcgag cgcgacgtca tgatccttct cgccaagggt
      901 ttgctcaaca agcaagtcgc ggcacaattg ggtttgagcg aaatcaccgt caaaatccac
      961 cgtggtcggc tgatgaagaa gatcggcgct tacacgctgg ccgatatcat caaaatggcc
     1021 gatctgctgc agcttcgcga tgctcccctt tcggggccgg aaaccggcag ttaagcgcct
     1081 atcttgcccc gccccgctca acctgcttaa aacccgccga aagcaggagc aaaaatgtcg
     1141 actttgaagc tattcgtttt gccgggcgac ggcatcggcc ccgaggtgat gaccgaggtc
     1201 gagaagatcg ccaatttcct caccaaacag ggcgtcgccc atttcgaaat cgagcgcggc
     1261 ctcgtcggcg gcgcggccta tgacgcgcat caagtcgcga tcagcgagga cgatatggcc
     1321 cgcgccggcg tagccgacgc catcctgctt gccgccgtcg gcggtcccaa atgggacaag
     1381 gtgccctatg acgtgcgccc cgaagccggc ctgctacggc tgcgcaagga tttcaatctc
     1441 tacgccaatc tgcgcccggc gatctgctat ccggcgcttg ccgacgcgtc ttcgctgaag
     1501 cgcgaactga tcgacgggct cgatctgctg atcgtgcgcg aattgactgg cggtgtctat
     1561 ttcggcgagc cgaaacaggt catcgatctt ggcaatggcc agaagcgcgc catcgatacg
     1621 caggtctacg acacctacga catcgagcgt accggccgca tcgccttcga gcttgcgcgc
     1681 aatcgccgca acaaggtgac ctcgtccgac acgcacaatg tgatgaaatc gggcgtgctc
     1741 tggcccgaga tcatcatcga tctgcacaag cgcgaattcc ccgatgtcga gc
//
LOCUS       DASWOG010000687         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_35849,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000687 DASWOG010000000
VERSION     DASWOG010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35849"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..570
                     /locus_tag="VGB93_14080"
     CDS             94..570
                     /locus_tag="VGB93_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0147245.1"
                     /translation="MSQTDQLRTESQPIFHSLALPRQALTGVRTRRVLAVTVDFVVVS
                     VLAFILWLGLLVGTLGLSLVVLPPLFPFVAFFYNGLTVSGRRMGTPGMQLMDLEIRRT
                     DGTRVPFLYAAVHAVLFYVSWMFPPILLVSLLAEDKRCLHDMLAGVVVTRRVRSLP"
     gene            675..1403
                     /locus_tag="VGB93_14085"
     CDS             675..1403
                     /locus_tag="VGB93_14085"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY0147246.1"
                     /translation="MTRELRDAPQFYLTSPSPCPYLPNREERKVFTHLVGRRATALND
                     TLTQSGFRRSQTIAYRPACENCRACVSVRVKVDEFQPSQNLRRVLKANQDVIAAVVRA
                     EPTSEQYSLFRGYLDARHSDGGMADMTVLDYSMMIEDSHVESRLIEYRLMKPAENNPL
                     LGVCLTDFLSDGLSMVYSFYEPDEEKRSLGTLIVLDHIERARQLGLPNVYLGYWVEGS
                     KKMAYKARFLPQERLGMNGWQRFE"
     gene            complement(1465..2952)
                     /locus_tag="VGB93_14090"
     CDS             complement(1465..2952)
                     /locus_tag="VGB93_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0147247.1"
                     /translation="MQIQLPHATEIGAALLGWRATNPAVDRMCIRYPEPHDALRHLAL
                     SLLIQEKIDPAIGFLKAALALAPQETVLWNDLAGAFYRASRQEEARAAQRTSLDLNVA
                     QPHAWLLLASVESNLGEDAAAESDYLTALRLDPHLAEAAFGLGLIYFQRRRFTESVKW
                     LRQSIADGGHNMGLYVCLGQALFLLGDFAEAVAALETAVRFPACDSTVVEKLAQLRLI
                     ETCVRDSAEAAVAAYAEIAGAHARAIDAVTLTAFQFLSGFGYRAAAIKLGEWRLARDP
                     GNTMQTYLLAALRSESLARAPDNYLVAYFNEFAETFESKLVNALDYHVPEKLYAMLAK
                     GGRRFANILDLGCGTGLCAPFLQQLGDRLTGVDLSRRMLEKASARALYSDLVEAEAGD
                     FLDRATDRFDLVVAADFVPYFGDLVTLFEKTARVLKPGGLFAFNAEKAERHFCILPSG
                     RFAHATSYIDAAAAANFKVADLQHTMIRLEAAKPVDGVLVVLQRL"
     gene            complement(3021..5264)
                     /gene="parC"
                     /locus_tag="VGB93_14095"
     CDS             complement(3021..5264)
                     /gene="parC"
                     /locus_tag="VGB93_14095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY0147248.1"
                     /translation="MGKNVVPPPAREPEPVNLRAALEERYLAYALSTIMGRALPDARD
                     GLKPVHRRILYGMRILRLDPGTAFKKCAKIVGDVMGSFHPHGDQAIYEALVRLAQDFA
                     SRYPLVDGQGNFGNIDGDGAAAYRYTEARMTEVARLLLEGINDDAVDFRDNYSGDQTE
                     PVVLPAAFPNLLANGSQGIAVGMATSIPPHNVAELCDACVHLIAHKSATDEDLLKFVP
                     GPDFPTGGIIVDSKEQIAETYRTGRGSFRVRAKWEREEGTRGTYNVVVTEIPYMVQKS
                     RLIEKIAELLGDKKLPLVADIRDESAEDVRIVIEPRVRTVDPALMMEQLFKTTELESR
                     FPMNMNVLVDGVIPRVVSLKEALRQWLDHRRTVLQRRSRFRLTEIAHRLEVLAGMRVV
                     FLNLDEVIRIIREEEEPKDFLKAAFDLTDVQANYILDTRLRSLRRLEEMELRREFDEL
                     TKEKEDIDKLLADDAKQWKVIGVELRDLKKKFGQDTSLGKRRTQFADAPEVADFDLAA
                     AMIEREPITVVVSEKGWIRALKGHVEDLSSLQFKGDDRLKQSFFTETTEKILVFATDG
                     RIFTLEASKLPGGRGQGEPLRLMVDIGEGEDIVSVFPYVAGEKMLVASNDGRGFIAPC
                     DEMVGGTRKGKMLLNRDPPAEASIVTPAAGDYVAVIGQNRKLLVFPIDQMPEMARGKG
                     VRLQRYREGGIADAKVFGKAEGLTWKDSAGRTFTVAMRDLTEWIGSRAEAGRLPPKGF
                     PKNNKFG"
     gene            5517..5735
                     /locus_tag="VGB93_14100"
     CDS             5517..5735
                     /locus_tag="VGB93_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147249.1"
                     /translation="MGKIVLFLGAVISAAIFSIALVGAPANGSAASEVQKNVQTGAAG
                     GCHVEEFALDEGYGVTRKMTHTVCKGME"
     gene            complement(5741..6460)
                     /gene="recO"
                     /locus_tag="VGB93_14105"
     CDS             complement(5741..6460)
                     /gene="recO"
                     /locus_tag="VGB93_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY0147250.1"
                     /translation="MEWHDDGLIIGLKKHGETSLILEAMTRAHGRHLGLVRGGRSKRW
                     QPLLQPGNSVALVWRARLDEHLGLYAAEVVKPRAANLMASALALHGLNHLATLLRLLA
                     ERDPHPALYETASTIADHLSETRTAAALVRFELAILAELGFGLDLATCAATGVREDLI
                     YVSPKSGRAVSRGSGAPYHERLLPLPTFLGDESVEGGSAAVAEAFRLTGYFLERDVFQ
                     ARELQMPHARQAYLAELAKLK"
BASE COUNT         1248 a         2009 c         1980 g         1281 t
ORIGIN      
        1 aattctgaat tcgcggcaat gtttccgttt gctcttgtct tgccgcaaga cgagggccac
       61 attcgggcgg cttacgccga ttaggaacaa gctatgagcc agaccgatca attgcggacc
      121 gagtcgcagc cgattttcca cagcctcgcc ctgccgcggc aggcgctgac aggcgtgcgg
      181 acacgccggg ttttggccgt gaccgtcgat ttcgtcgtgg tttccgtcct cgcgtttatc
      241 ctttggctcg gcctgctggt cggaacgctc ggcctgtcgc tggtcgttct gccgccactt
      301 tttccgttcg tcgcgttctt ttacaacggc ctgacggtct ccggccggcg catgggaaca
      361 cccggcatgc agctcatgga tctcgaaatc cgccgcaccg acggcacgcg ggtgcctttc
      421 ctctatgcgg cggtccatgc ggtcctgttc tacgtgagct ggatgttccc gccgatcctg
      481 cttgtctcct tgctggcaga ggataaacgc tgcctgcatg acatgctggc gggcgtggtg
      541 gtcacgcgcc gggtgcgtag tcttccctga gaatttttct aggcgcagag cgcgggacgc
      601 gttagccttc gcatgtgggc cgcttcgctg gcccggacgg cctgaagccg tgacagaagt
      661 ttgaagaggc aggcttgacg cgagaactgc gcgacgcgcc gcaattttat ctcacttcgc
      721 cttcgccgtg cccttacctg ccgaatcgcg aagagcgcaa ggtctttacc cacctcgtcg
      781 gccggcgggc gacggcgctg aatgacacat tgacgcaatc cggcttccgc cgctcgcaaa
      841 cgatcgccta tcgcccggcc tgcgaaaatt gccgtgcctg cgtttctgtg cgcgtgaagg
      901 tggatgaatt ccagccgtcg cagaatttgc gacgcgtgct caaggccaat caggacgtca
      961 tcgccgccgt agtgcgcgcc gagccgacga gcgagcaata ttcgctgttt cgcggatatc
     1021 tcgacgcccg ccacagcgat ggcggcatgg cggatatgac tgtgctcgat tattcgatga
     1081 tgatcgaaga cagccatgtc gaaagccggc tgatcgaata tcgcctcatg aaaccggccg
     1141 agaacaatcc tttgcttggc gtctgcctga cagacttcct ctcggacggt ctctcgatgg
     1201 tctattcctt ttatgagcca gatgaagaaa aacgttcact cggcacgttg atcgtcctcg
     1261 atcacatcga acgcgcaagg cagctcggcc tgcccaatgt ctatctcggt tattgggtcg
     1321 aaggctctaa gaagatggcc tataaggccc gcttcctgcc gcaagagcgg cttggcatga
     1381 acggctggca gcgcttcgag taaagtatcg gatcgaaaag cgcgaagtag ttttcggaca
     1441 aatccgatgc gcttaaaaag ccgcttagag ccgctgcagc acaaccagga cgccgtccac
     1501 cggctttgcc gcttcaagcc gaatcatcgt gtgctgcaaa tccgctacct tgaaattcgc
     1561 cgctgctgcc gcgtcgatat aactcgtcgc gtgggcgaag cggcctgacg gcaagatgca
     1621 gaagtggcgc tctgcctttt cggcattgaa ggcaaagagc ccgcccggct tgagtacgcg
     1681 cgcagttttt tcaaaaagcg tgacgagatc gccaaagtaa ggcacgaagt cggctgcaac
     1741 gaccaagtcg aatcgatccg tcgcacggtc gagaaaatca cccgcttcgg cctcaacgag
     1801 atcgctgtaa agtgcgcgtg cactggcttt ttccagcatc cggcgcgaaa ggtcgacacc
     1861 cgtgagacga tcgccaagct gctgtaaaaa cggcgcgcaa aggccggtgc cgcagccgag
     1921 atcgaggatg ttggcgaagc ggcgaccgcc cttggccaac atggcgtaga gtttctcagg
     1981 cacgtgataa tcaagcgcat tgacgagctt ggattcaaac gtctcggcaa attcgttgaa
     2041 atacgcgacg agataattat ccggcgcgcg ggcaagcgat tcgctgcgca aagcggcgag
     2101 aagataagtc tgcattgtgt tgccgggatc gcgcgcgaga cgccattcgc cgagtttaat
     2161 cgccgccgcg cgatagccga agccgctgag aaattggaaa gccgtgaggg tcacggcgtc
     2221 gatagcgcgc gcatgcgccc cggcaatctc ggcataggcc gccacggcag cttcggcgct
     2281 gtcgcgcacg caggtttcga tcagccgcaa ttgcgccagc ttctcgacga cggtgctgtc
     2341 acaggccgga aagcgcacgg cggtctcgag cgccgcgacg gcctcggcaa aatcgccgag
     2401 caggaacagc gcctgcccga ggcagacgta aagccccatg ttgtgtccgc cgtcggcgat
     2461 cgactggcgc agccatttga cgctttccgt gaagcggcgg cgctggaaat agataagccc
     2521 gagaccgaag gcagcttccg cgagatgcgg atcgagcctg agcgccgtca ggtaatccga
     2581 ttccgccgcg gcgtcttcgc cgaggttgct ttcgaccgag gcaagtagca gccaggcatg
     2641 cggctgcgcg acgttgaggt cgagcgacgt ccgctgcgcg gcacgcgctt cctcctgccg
     2701 gcttgcgcgg tagaaggcgc ccgcgagatc attccacaaa accgtctctt gcggcgccag
     2761 cgccagcgcc gctttgagaa acccgatggc cggatcgatc ttctcctgaa tcagaagcga
     2821 aagggcgaga tgccgcaaag cgtcatgcgg ctcggggtag cggatgcaca tgcggtcaac
     2881 ggctgggttc gtggcgcgcc agcccaaaag cgcggcgccg atttccgtcg cgtgcggcag
     2941 ttggatctgc atgatcaatg tcctcgcgcc cgtctgtttg cgaggattag gaccgcgagc
     3001 ttgcctctgc ctgtcgcaga tcagccaaac ttattattct tagggaaacc cttcggtggc
     3061 aggcggccgg cctcggctcg gctgccgatc cactccgtca aatcgcgcat tgccacggtg
     3121 aatgtgcggc cggccgaatc tttccaggtc aggccctccg ctttgccgaa gaccttggcg
     3181 tcggcaatgc cgccctcacg atatcgctgc agacgaacgc ctttgccgcg cgccatctcc
     3241 ggcatctgat cgatcgggaa gacaagcagc ttgcgattct ggccgatgac ggcaacgtag
     3301 tcaccggcag caggcgtgac gatcgaggct tcggccggcg ggtcgcgatt gagcagcatt
     3361 ttgcctttgc gggtgccgcc aaccatttca tcgcacggcg cgatgaaccc gcgcccatcg
     3421 tttgaggcaa caagcatctt ctcgccggcg acatagggaa agacggagac gatatcctcg
     3481 ccctcgccaa tatccaccat tagccgcagg ggctcgccct gcccgcgccc gcccggcagt
     3541 ttcgaggctt ccagcgtgaa gatacgaccg tccgtcgcga agacgagaat cttctcggtc
     3601 gtctcggtga agaagctttg cttcaatcgg tcgtcgccct tgaattgcag gctcgacaag
     3661 tcctcgacgt ggcccttgag ggcgcgaatc cagcccttct cggagacgac gaccgtaatc
     3721 ggctcgcgct cgatcatcgc cgccgcgaga tcgaaatctg cgacttccgg cgcgtcagcg
     3781 aattgcgtgc ggcgtttgcc tagcgacgta tcctggccga actttttctt cagatcgcgc
     3841 agctcgacgc cgatgacctt ccattgcttc gcgtcatcgg cgagcaattt gtcgatgtcc
     3901 tctttttctt tggtcagctc gtcgaactcg cggcgcaatt ccatctcttc aagtcggcgc
     3961 aggctgcgca gccgcgtatc gagaatataa ttggcttgaa cgtcggtgag atcgaacgcc
     4021 gctttcagga aatccttcgg ctcttcctct tcgcggatga tgcggatcac ttcatccaga
     4081 ttgaggaaga cgacgcgcat gccggcaaga acttcgagcc gatgcgcgat ttcggtcagc
     4141 cggaagcgcg aacgccgctg cagcacagtg cggcgatgat cgagccattg ccgcaatgcc
     4201 tccttgagcg ataccacacg ggggatcacg ccatcgacga gcacgttcat gttcatcggg
     4261 aagcggcttt caagctcggt cgtcttgaaa agctgttcca tcatcagcgc cggatcgacc
     4321 gtgcgcacgc gcggctcgat gacgatgcgc acatcctcag ccgattcgtc acggatgtcg
     4381 gcgacaagcg gcagtttctt gtcgcccaga agttcggcga tcttctcgat cagccgcgac
     4441 ttctgcacca tgtaggggat ttcggtaacg acgacattgt acgtgccgcg cgtcccctcc
     4501 tcgcgctccc atttcgcccg cacgcggaag gagccacggc cggtgcgata cgtctcggcg
     4561 atctgctctt tcgaatcgac gatgatgccg ccggtcggaa agtccggccc cggcacgaat
     4621 ttcagcagat cctcgtcggt cgcactttta tgcgcgatga gatgcacgca ggcgtcgcaa
     4681 agctccgcca cattatgcgg cgggatggaa gtcgccatgc caacggcaat gccctgcgac
     4741 ccgttggcga gaagattggg gaaagccgcg ggaaggacga ccggctcagt ctggtcgccg
     4801 gaataattgt cgcggaaatc gaccgcgtcg tcgttgatgc cttcgagcaa cagccgggcg
     4861 acttccgtca tccgggcttc ggtgtagcga taggccgcgg cgccgtcgcc atcgatattg
     4921 ccgaaattgc cctgcccgtc cacgagcgga tagcgcgagg cgaaatcctg cgcgagacgc
     4981 accaacgcct catagatcgc ctgatcgcca tgcggatgga acgaacccat cacgtcgccg
     5041 acgatttttg cacatttctt gaaagcggtg ccggggtcga gacgcaagat gcgcatcccg
     5101 taaagaatgc gccggtgcac cggctttaga ccgtcgcggg catccggcaa cgcccggccc
     5161 atgatcgtcg agagcgcata ggcaaggtaa cgctcttcaa gcgccgcgcg cagattgacg
     5221 ggttccggct cgcgcgccgg gggcggaaca acatttttgc ccatgagatt gccttttcgg
     5281 gatcacccga ccgccccccg gcgggaacga atgtctagag gatgaattga gtcgccgcaa
     5341 tctgctctgc cgcagtgcgt gaagggtctg ctctgttgcg tggctgcact agccgcccgg
     5401 gtgctgacgc ccttaaaagg atgccgcgac tcggaggcgt gaagtgtgcc gccgcaccgt
     5461 ttcagccgat atttatggca ggttgcgcag gattttccgc gggggtccag gttgtgatgg
     5521 gcaaaatcgt tttgttcctg ggtgccgtga tcagcgcggc gattttttca atcgcgctgg
     5581 ttggggcgcc ggccaatggc agcgccgcca gcgaagtcca aaagaacgtt caaaccggcg
     5641 ccgccggtgg ctgccatgtc gaggaatttg cgctcgacga aggctatggc gtcacccgca
     5701 aaatgaccca caccgtttgc aagggcatgg aataagcccc tcattttaat ttcgctagct
     5761 cggccagata ggcctgccgc gcgtgcggca tttgaagctc gcgcgcctgg aagacatcgc
     5821 gctccagaaa atagccggtg agccggaacg cttccgccac cgcggctgag ccgccctcga
     5881 cgctttcatc gcccaaaaag gtcggcagcg gcagcaaccg ttcgtgatag ggcgcgccgg
     5941 aaccgcgcga taccgcgcgg ccgctcttcg gcgacacata gatcaagtct tcgcgcacgc
     6001 cagtcgcggc gcaagttgcg aggtcaaggc cgaagccgag ttccgcgagg atcgccagtt
     6061 cgaagcgcac caacgcggcg gcagttcgcg tttcgctgag atgatcggca atcgtcgatg
     6121 cagtctcgta aagtgccgga tgcggatcgc gctcggccag aagtcgcagc aaagtggcaa
     6181 gatgattgag gccgtgcaac gccagcgcgc tcgccatcag gttcgcggcg cgcggcttca
     6241 ccacctccgc cgcataaagc ccgagatgtt catcgagccg cgcccgccag acgagcgcca
     6301 cgctattgcc gggctgcagc aggggctgcc agcgcttcga ccgcccgccg cgcacaaggc
     6361 cgagatggcg accatgcgcc cgtgtcatcg cttcgaggat caggcttgtc tcgccgtgct
     6421 tcttgaggcc gatgatcagt ccgtcgtcat gccattccat gcgggcctag gcctcgtcca
     6481 cgtcgtagcg atagccgacg ccgtgaaccg tgcggatg
//
LOCUS       DASWOG010000688         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_17631,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000688 DASWOG010000000
VERSION     DASWOG010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17631"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGB93_14110"
     CDS             complement(<1..232)
                     /locus_tag="VGB93_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0147251.1"
                     /translation="MKAVKPRRKPLLGTLVSLLIIAGAIAFGVHTIRQVWRYPSTDDA
                     RIDADVVHIAALVGGRIVTIAVTENAHVAKGDL"
     gene            complement(234..437)
                     /locus_tag="VGB93_14115"
     CDS             complement(234..437)
                     /locus_tag="VGB93_14115"
                     /inference="COORDINATES: protein motif:HMM:NF028400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HEY0147252.1"
                     /translation="MAPDFYLFGTFFPAWMLCAIIAIFSGILIRVLFVASGIGRVLPF
                     QLFVCTAVGAIAGTLTWSIWFGP"
     gene            844..1296
                     /locus_tag="VGB93_14120"
     CDS             844..1296
                     /locus_tag="VGB93_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147253.1"
                     /translation="MATDVAYISALSALAGSVIGGLASGLTTWLNRHAEAKANWLARD
                     ISRRQDLYRDFIVSASKAYGEAIVTDEPQIQELVELYGLVSRMRVLSRPKTIECADKV
                     LHTTIDTYFLPNKTFSELNDTLKNGAGIDPLKEFAVAAREELQGLTPV"
     gene            2001..>3166
                     /locus_tag="VGB93_14125"
     CDS             join(2001..2343,2343..>3166)
                     /locus_tag="VGB93_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611724.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0147254.1"
                     /translation="MLKHGLMSDIEIITDGGRRRHWSAADKLRIVEETLDDRASISVV
                     ARRHGVAPNLLYRWRRLMLEGGSVAVADDGEVTGNKTVRQMEERIRELERQLGKKTLE
                     VEILKEALDKSRFKKTDLAVGLVAEGRFAMKAVADTLGVARSNLYDRLKGETRPRRRY
                     HKPGDVELLPRIERLVTARPTYGYRRVTAVLNRELRAEGLSPANHKRVYRIMQMHQML
                     LARRYTERPDLVHDGKVIVMRSNLRWCSDGFEFTCWDGAIVRGAFIIDAHDREIIAWR
                     AVVNAGISGSDVRDMMLEAVERRFSSYRASAAVEILSDNGSPYIARETRIFAAQLGLK
                     PCFTPVKSPQSNGISEAFVNTLKRDYVRVTPVPNAETALKAMSAWFEDYNDHHPH"
BASE COUNT          704 a          864 c          933 g          665 t
ORIGIN      
        1 gcagatcgcc tttggcaaca tgggcgttct cggtgaccgc gatcgtgacg atccggccgc
       61 cgaccagcgc ggcgatatgc acgacgtcgg catcaatccg cgcatcgtcg gtcgaaggat
      121 agcgccagac ttgccggatc gtgtgaacgc cgaaggcgat cgctccggca atgatcagaa
      181 gcgaaacgag cgttccgagc agcggcttgc ggcggggttt gacggccttc atctcaaggc
      241 ccgaaccaga ttgaccaggt cagtgtgccc gcgatggcgc cgaccgctgt gcagacgaaa
      301 agctgaaatg gcaagacgcg cccaatgcct gacgcgacaa aaagaacgcg gatgagaatg
      361 cccgaaaaaa ttgcgatgat ggcgcacaac atccaggctg ggaaaaacgt cccaaataaa
      421 taaaaatcgg gcgccatgac actcaaacgg ctgggaaatt tcgagtagat tttgtcttca
      481 gaaccaacac agttcaatcg cggcgaccag cagaattatg ggatgtgaca atatttctcc
      541 gccttgtgtc gtggaggcca ttgcctatta cgatcgatag atcaaatcaa aaataatgta
      601 tagctagtta tatgtcgact atgggaagac tagttgaaat ataataatgt attatttacg
      661 taaaacgaaa tcacgccgct acatattttg gcattttctg ataagttttc gcgcccgaat
      721 cggccgatgg tttatatttt tgaggggctg gcctgccttg cagcgaacgg tcggttgcat
      781 agaaaacagc ttcgacgtca gtcgataagg agtcggcggc gtccattttg ggtggagagc
      841 agggtggcca cagacgtcgc atatatttcg gcgctatccg cgctcgccgg gtccgtcatc
      901 ggcggtctcg cctccgggct gacgacgtgg ctcaatcgac atgcggaagc gaaagcgaac
      961 tggctcgcca gggacatttc ccggcgccag gatctctaca gggattttat agtttccgca
     1021 tccaaagctt atggcgaggc gatcgtcact gacgagcccc aaatccagga gcttgtggag
     1081 ctttacggtt tggtcagcag aatgcgcgtt ctatctaggc ctaagacaat tgaatgcgct
     1141 gacaaagttc tccatacgac cattgataca tatttcctgc cgaataagac cttttccgaa
     1201 ttgaacgata ctttgaaaaa cggtgccggc atcgacccgc tgaaggaatt cgccgtagcc
     1261 gcgcgcgagg aattgcaggg cctcacgcca gtctgaacat gccgcggccg gcgtggcata
     1321 aggtgggtag cgaagcgcgc tatttcgcgc cgtcgagggt tttgttgcag ctaccagctt
     1381 taggatgagg gccgctttgc ggcagtgctc tatctattgg attttttatc gcttgtggcg
     1441 ggcgcacaaa agctcaaact ttggactcgc gaattaagag tcttaagcca tagctaagaa
     1501 aacaagatgt tcccgtcatc ggatgcgtcc aacgcggcat gatcgaataa agggttagcg
     1561 gcgacttgtc caacgttgct ttggccactt tttgggtggg cccagccaat ttgattgctg
     1621 atgtcggatt cattcgatgg atacttgctt agttccgatg acggcactcg tttaggaaga
     1681 ggccgccaac tgagggggcg gccttcctcc gctatgggca gcacttctga tcgagccttc
     1741 tttcctggct ccatctgtgg actggaccga tcctcgtcgt cgtttctttc gctagcgtgg
     1801 cgcggcctta agtgatcttg aggcgctgga gtattggcac cttcaccaca tcgaggatga
     1861 tcgcaaagat aacggccgca gccagcgtgc cgaccaccgt ccaggccggc aaaggcgcca
     1921 ttgcgattcc gccgatggcg agggtctgat ctggcccccg tttgaaccgg acactcaagc
     1981 ctaactggga acttggggat atcctcaagc atgggcttat gtctgatatt gaaatcatca
     2041 ccgacggtgg gcgtcgccgg cattggtcag cggcggataa actccggatt gtcgaggaga
     2101 cgctcgacga ccgcgccagc atctcggttg tcgctcggcg gcatggtgtg gcgccgaacc
     2161 tgttgtatcg ttggcgccgg ctgatgcttg agggcgggag cgtggcggtg gctgatgacg
     2221 gcgaagtgac cggcaacaag acagtccggc agatggaaga gcggatccgc gaactggaac
     2281 gccagctcgg caagaagacg cttgaagtcg aaatcctgaa ggaagcgctg gataaatcgc
     2341 gctcaaaaaa acagatcttg ctgtcgggct cgtcgcagaa ggacggtttg cgatgaaggc
     2401 ggtcgctgac acgctcggtg ttgctcgctc gaacctctac gaccggctga agggcgagac
     2461 ccgtccgcgc cgtcgctatc ataagcccgg tgacgtcgag ctgctgccgc ggatcgagcg
     2521 actggtcacc gcccgcccga cttatggcta caggcgggtc actgcggtcc tgaaccgcga
     2581 attgcgcgcc gaaggcctat cgcccgcgaa ccataaacgg gtctaccgca tcatgcagat
     2641 gcatcagatg ctgctagcgc gccgttacac agagcgcccc gacctcgtcc atgacggcaa
     2701 ggtcatcgtg atgcgatcga acctgcgttg gtgctctgac ggcttcgagt tcacctgttg
     2761 ggatggcgct atcgtccgcg gcgccttcat catcgacgcg cacgaccgcg agatcatcgc
     2821 ctggcgggcg gtggtgaacg ccggaatcag cgggtccgac gtgcgcgaca tgatgctgga
     2881 ggcggtcgaa cgacgcttca gcagctatcg ggcttccgcg gcggtcgaaa tcctgagcga
     2941 caacggctcg ccctatattg ctcgggaaac caggatcttc gccgctcagc tcggcctaaa
     3001 accctgcttc acgccggtga agagccctca gagcaacggc atctcggagg ccttcgtcaa
     3061 cacgctgaaa cgcgactatg ttcgcgtcac accagtcccg aatgcggaga ccgccctaaa
     3121 ggcgatgtcc gcctggttcg aggattacaa cgaccatcat ccacat
//
LOCUS       DASWOG010000689         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_182195,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000689 DASWOG010000000
VERSION     DASWOG010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182195"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VGB93_14130"
     CDS             complement(<1..671)
                     /locus_tag="VGB93_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY0147255.1"
                     /translation="MPDTPIKALFFDVFGTLVDFRSGVAREAERLIAPKGKILDWGAF
                     ADAWRGEYQAGMEEIRSGREGYVRLDVIHRRNLNRILPRFGLEYLNEDTRADLNLAWH
                     RLDAWPEVAAALAQLRAQFRIAPLSNGNIALQVALARHNDFLWDTILGGDFSRDYKPK
                     PQLYLDAVDAFGFRPAECLMVACHSTDLAAAASHGLRTAHIARPDEHGVGRGETAPSV
                     TVDFTA"
     gene            complement(790..954)
                     /locus_tag="VGB93_14135"
     CDS             complement(790..954)
                     /locus_tag="VGB93_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147256.1"
                     /translation="MREMFGFSALGALGGAVLFRRPVSPTSLCIWDHAAVMLLITLLL
                     SVALRLTQIA"
     gene            complement(1465..1620)
                     /locus_tag="VGB93_14140"
     CDS             complement(1465..1620)
                     /locus_tag="VGB93_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147257.1"
                     /translation="MPNVVRPISVYTGEMDLFGGGPEKVTRENMAKLIDAEVENAARS
                     PSAKSAG"
BASE COUNT          426 a          531 c          565 g          380 t
ORIGIN      
        1 gctgtaaaat cgaccgttac acttggggcg gtttcaccgc gacccactcc atgttcgtca
       61 gggcgcgcga tatgcgcggt gcgcagcccg tggctcgcgg ctgccgcgag atcggtgctg
      121 tgacaggcga ccatcaaaca ttccgccggt ctgaaaccga aagcatcgac agcatcgaga
      181 taaagctgcg gtttgggttt gtaatcgcgc gaaaaatcac cgccgagaat tgtatcccaa
      241 agaaaatcat tgtggcgggc gagagcgacc tgcagggcga tgtttccgtt ggacagcggc
      301 gcaatacgaa actgcgctct tagctgcgcc agcgcggccg ccacctctgg ccacgcgtca
      361 agtctatgcc aggcgaggtt cagatcggcg cgcgtgtctt cattgagata ttcgaggcca
      421 aagcgcggga gaatacgatt gaggttgcgg cgatggataa catcgagtcg gacgtagcct
      481 tcgcgcccgg agcggatttc ttccatgcct gcctgatatt cgccgcgcca ggcatcggcg
      541 aacgcgcccc aatcgagaat cttgcctttg ggcgcgatga gccgctctgc ctcgcgcgcg
      601 acgccgctac ggaagtcgac gagcgtgccg aagacatcga aaaagagcgc ttttatcggc
      661 gtgtcgggca aggtccacct ctcggagttt gaggaccttc attcgcgcgg ttttgattat
      721 tcgtccagtg ggcggggcga gttagctgaa cgacgacgag ggaatgaaag gtattagcct
      781 accagaccac taggcaattt gcgtcaatcg gagggccacc gaaagcaaga gcgtgatgag
      841 cagcatgacc gctgcatgat cccagatgca aaggctcgtc ggggaaaccg ggcgacgaaa
      901 taaaacggca ccgccgagcg cgccgagcgc cgagaagcca aacatttcac gcatgactaa
      961 aaagaaaagc gccgtttgtg ctgatctaag aacaaggcag gtacgcaaaa tagcaccacg
     1021 aaggcagttt gacgcgcgaa taacctcaga tgaactctgg aaagctcatt gggctcgcta
     1081 agcatcctcg cccaccgaag tcgcgcaata tccacaagca tttaggtttg ccgccgattg
     1141 ctaaactatg tgagcgagtt tgatggcgac ctgtatggcg cccgacagca gcacgaaagc
     1201 gctaacggct agaataatac gacccaccac tgcgccgggc cggttattcg ccgcagcgtc
     1261 gcgggcatat tcgttccagt cctcagattg tgaatttgct gcaccgcgcg cgtaaaacgc
     1321 gtcccgttct gcaagtgcgc gttcaatagt tgcgctacgc attgtctagc tgccgtcagt
     1381 ctaaatccca tggtcagcct agacgcgtcg cgcatataaa ttcgatgtgc gtctcatcgc
     1441 tggaaaatat atgccatcat tcgcttatcc ggcagactta gcggacgggc tgcgtgccgc
     1501 gttttcgact tccgcgtcaa tcaacttggc catgttttcg cgcgtgactt tctcagggcc
     1561 gccgccgaaa aggtccattt cgccggtata gacggagatc ggcctgacga cgtttggcac
     1621 gcccttgcct cgcgtctgca ttgtatcggc aaaccgcgcc accacgatcg ggccttgttt
     1681 agcttcgatc gtaacaaggg atagtccgca taatgccgga gtgcgtgcgc cactagcgac
     1741 gctgttcgcg cggtcatctc catcgggcgg ccagaggccc gctcgaatct ttgcgcgagg
     1801 atgagaattt cagacggttg agggcgggcg cgcataagaa cggatagaga acaggccggg
     1861 cctgagtcaa tcgcgattcg ggcgggaaat ggtgggcggt ac
//
LOCUS       DASWOG010000690         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_364653,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000690 DASWOG010000000
VERSION     DASWOG010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364653"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..>1684)
                     /locus_tag="VGB93_14145"
     CDS             complement(280..>1684)
                     /locus_tag="VGB93_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0147258.1"
                     /translation="YAARDYAPLWIENNKPVAAVAPIKARIALASDDALNLAATPQAD
                     FSGDADHLAAAEIAFSDEIVAYGRQASGVRLDPQEINPLIGAKPDVAAPGLILGAVAA
                     ADTDGSAVLQGFNPQQKPYAALREKLMALRHQNDAVSDRTIPPGPVLKLGMSDPRVPL
                     IRARFGLDVTQSDDDLIYDTQVAAAVADFQRANGLRPSGILTPRTQASLSGEEPKPDV
                     GNDIIANMEIWRWMPHDLGENRIDVNIPDYEVRVFHDGKVVEENKVVVGKSTTPTPVF
                     SNTMKFAIINPYWNVPPSILRKEMLPRLAHDPGYLQRMGFETYYFHGQLMVRQPPGEK
                     NALGRIKFMFPNQYSVYLHDTPEKRLFASSKRAFSHGCVRVDQPLDFAQTVLGPKWPQ
                     ERIESLIGGKERYVFLPTPLPIHIEYFTAYVSDDDRLVLRNDVYGYEHKIEEALGLIT
                     PKPTNRNVAAQFVPSRY"
BASE COUNT          338 a          484 c          514 g          348 t
ORIGIN      
        1 agccgatctg cttattcggg cgtctccgcc gatacctgca tttaccgaga accgtcagcg
       61 cttctggaac gaaacgctac ccccgttcaa cggtcgcctc acatccctga cactattgat
      121 gttgccgaat gacgaagacc agttcaggta agccagcaaa tgtggcgaac ctgtgtcggc
      181 gagcgagctt tctcttcacc ctgaatcgcg gacgggagcc gaaacgaacg ccgcttttcg
      241 tttcaattcc aaaagaatcg ctgattcgac gcctcgccgt caatatcggg acggaacgaa
      301 ttgcgcggcg acgttccgat tcgtcggttt cggcgtgata agaccgagcg cttcctcgat
      361 cttgtgctca tagccataca cgtcgttgcg caggacaagt cggtcgtcat cgctcacata
      421 ggcggtgaaa tattcgatat ggatcggaag cggtgtcggc aggaacacgt aacgctcctt
      481 gccgccaatc aagctttcga tgcgctcttg cggccatttc ggtccgagca ccgtttgcgc
      541 aaaatcgagc ggctgatcga cgcggacgca accatgactg aaagcgcgtt tcgacgaggc
      601 gaacaggcgc ttctccggcg tatcgtggag atagaccgaa tattgattgg ggaacatgaa
      661 cttgatacgg cccagcgcat tcttttcgcc cggcggctgg cgcaccatca attgaccatg
      721 gaaataatag gtctcgaagc ccatgcgctg gagatagccg ggatcgtgcg cgagacgcgg
      781 cagcatttcc ttgcgcagga tcgacggcgg cacgttccaa tacggattga tgatcgcgaa
      841 tttcattgtg ttggaaaaga ccggcgtcgg cgtcgtggat ttgccgacga caaccttgtt
      901 ctcctcgacg accttgccgt cgtgaaaaac ccgaacttca taatcgggga tattcacatc
      961 gatgcggttc tcgccgagat cgtgcggcat ccagcgccag atttccatat tggcgatgat
     1021 gtcgtttccg acgtcgggct tcggctcctc gcctgaaagg ctggcctgcg tgcgtggggt
     1081 aaggatgcca gacgggcgaa gcccgttggc gcgctgaaaa tcggcgaccg ctgcggcaac
     1141 ctgcgtgtcg tagatcaggt cgtcatcgct ttgcgtgaca tcaaggccga aacgggcacg
     1201 gatcagcggc acgcgcggat cgctcatgcc gagcttgaga acggggccgg gtgggatcgt
     1261 gcgatctgaa accgcatcgt tttgatggcg cagcgccatc agcttctcgc gcagggcggc
     1321 gtagggcttc tgctggggat tgaagccctg caaaactgca ctcccatccg tatctgccgc
     1381 ggcgaccgca ccgagaatga ggccgggcgc cgcgacatct ggcttggccc cgatgagtgg
     1441 attgatttcc tgcggatcga gccgcacgcc cgaagcctgg cggccgtagg cgacgatctc
     1501 gtccgagaag gcgatttcgg ccgcggcgag atgatccgca tcaccggaga aatcggcctg
     1561 cggggtcgcc gcgagattaa gcgcgtcgtc gctcgccagc gcgattcgtg ccttgatcgg
     1621 ggcgacagcc gcgaccggct tgttattttc gatccagagc ggcgcatagt ctcgggcggc
     1681 gtaa
//
LOCUS       DASWOG010000691         4490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_71752,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000691 DASWOG010000000
VERSION     DASWOG010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71752"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2942)
                     /locus_tag="VGB93_14150"
     CDS             complement(<1..2942)
                     /locus_tag="VGB93_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0147259.1"
                     /translation="MTISAAIHHVTHYRYDRPIEVGPQTIRLRPAPHCRTKIASYSLK
                     ITPPDHFINWQQDPHGNWLARVVFPEKTSELKIEVDLTAVLAVINPFDFFIEPSAEQF
                     PFAYAPELKKELAPYLEAEPLGPRVDEYIAALLKGPIHTITFLVDLNANLQNAVRYVI
                     RMEPGVQTPEETLALASGSCRDSAWLLVQILRRLGLAARFVSGYLIQLRADVDPIEGP
                     KGTQHDFTDLHAWAEVYIPGAGWIGMDATSGLFCGEGHIPLCATPHYRSAAPISGAVE
                     PAQVEFGFEMNVTRVAEAPRITLPFSEEAWDELNVLGKKIDADLVAQDVKLTMGGEPT
                     FVSIDDFEAPEWNTAAVGPTKRSRADELIRRLRTRFAPGGMLHYGQGKWYPGETLPRW
                     AFALYWRKDGKPIWKDPALIAPVPVTRTAEEIKTMPRDAAAEEALRVQAEKLTAGIAA
                     RLGIEGDCIIAAYEDPTTWLVKEGNLPENTDPLDPKIEDAEERNRMVRTFARGLTTAS
                     GFVLPVQRWNAQAETQSRWTSERWKLRRGRLFLVPGDSPVGYRLPLSSLPWVPPASYP
                     FIVSQDPLEDRGSLPDPMDIGQQFERVASDGAAHQDRVEQQIVEGAVRTALSVEPRDG
                     ILCIFMPPVEKLADYLELLAAAEATAASLKTPVHIEGYPPPFDPRLDVIKVTPDPGVI
                     EVNVHPAKSWDEAVATTKGLYEDARQSRLGADKFMTDGRHTGTGGGNHIVFGGPTPQD
                     SPFLRRPDMLKSLVLYWQRHPALSYLFSGLFIGPTSQAPRFDEARLDQLYELEIALAS
                     VPSPQQKAPLWVVDRLFRNLLVDSTGNTHRTEICIDKLYSPDGPTGRLGLVEFRSFEM
                     PPDARMSLAQQLLLRGLLAWFWREPQNGEFVRWGTALHDRFMLPHFVWENFLEILDDL
                     KRAGYDFDPLWFEAQREFRFPVYGVVERGGVTLELRQALEPWHVLGEEGTIGGTVRYV
                     DSSVERLQV"
     gene            3145..4380
                     /locus_tag="VGB93_14155"
     CDS             3145..4380
                     /locus_tag="VGB93_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY0147260.1"
                     /translation="MNFRRILKASPIFVALASFYGPALSQAPASPRPEDALVAAYNAT
                     GERLFQDLAQEPGNIVLSPYSIGSALSMVLAGARGQTAAATSDTRGPHLSATDLAAAN
                     ASLIANLKAAGGKDSVTLNSANALIEVRPGIVSADYRALLQKSYGAEAFSGDLDAINR
                     WVAQKTSGKIPHILDSLPDGTGFVLANAAYFKAFWQHPFDKHATEDGTFHLTALKPVQ
                     VPMMHNEDHFALVKGNGYKAIRLPYRSDGERLSMIVVIPDAINGLGALIAGLGPDQLA
                     RLLADLNTADFVDVRLQVPRFHADFQASLAAPLQETGMAAAFDLQKADFRGITDGSLA
                     AQQVAMGDVIHSAVVDVNEESSEAAAATVVPMIATAMAPQYEPVPEPFIVDRPFLFFI
                     VDQQTGAVLFQGRIADPRK"
BASE COUNT          892 a         1397 c         1368 g          833 t
ORIGIN      
        1 acttgcaggc gctcgacgga agaatcgaca tagcggaccg tgccgccaat cgtgccctcc
       61 tcgccaagca catgccaggg ctcgagcgcc tgacgtaatt caagcgttac gccgccccgc
      121 tcgaccacgc catagaccgg gaaacggaat tcccgctgcg cctcgaacca tagcggatcg
      181 aagtcgtaac cggcgcgctt caggtcgtcg aggatttcaa gaaaattctc ccagacgaaa
      241 tgcggcagca taaaccggtc gtgcaatgcc gtcccccagc gaacgaactc gccgttctgc
      301 ggctcgcgcc agaaccaggc cagcagcccg cgcagcagca attgctgcgc gagactcatc
      361 cgcgcgtccg gcggcatttc gaacgagcga aactcgacaa ggccgagacg tccggtcgga
      421 ccgtccgggg aatagagctt gtcgatacag atttcggtgc gatgcgtgtt gccggtcgaa
      481 tcgaccagca gattgcggaa cagccgatcg acgacccaaa gcggcgcctt ctgctgcgga
      541 ctgggaacgc tggcgagggc gatctccaat tcgtagagct gatcgagccg cgcctcgtcg
      601 aagcggggcg cctggctggt cgggccaatg aacaggccgg agaagagata cgacagtgct
      661 ggatgacgct gccaataaag caccaggctc ttcagcatgt cgggtcggcg caggaagggc
      721 gaatcttgcg gcgtcggacc gccgaagacg atgtgattgc caccgcccgt gccggtatgg
      781 cggccgtcgg tcatgaattt gtccgcgccg aggcgcgact gacgggcatc ctcgtaaagc
      841 cccttggtcg ttgcgacggc ctcatcccag cttttggccg gatgcacatt gacttcgatg
      901 acgccgggat cgggcgtgac cttgatgaca tcgaggcgcg gatcgaacgg cggcggatag
      961 ccctcgatat gaaccggcgt tttcaacgat gcggcggtcg cctccgcggc tgcaagcagc
     1021 tcaagataat cggcaagctt ttcgacgggc ggcatgaaga tacagaggat gccatcgcgc
     1081 ggctcgacgg agagtgcggt gcgcaccgca ccttcgacga tctgctgttc gacccgatcc
     1141 tgatgcgcag caccatcgga ggcaacgcgt tcgaactgct ggccgatgtc catcgggtcg
     1201 ggaagactgc cacggtcttc cagcggatcc tgcgagacga tgaacggata ggatgcgggc
     1261 ggcacccagg gcagcgacga caggggcaag cgatagccca cgggggaatc gcccggcaca
     1321 agaaacagcc gcccgcgccg cagcttccac cgctcgctcg tccagcgcga ctgcgtctcc
     1381 gcttgcgcgt tccagcgctg cacaggcagt acgaagccgg aagccgtcgt gaggccgcgg
     1441 gcaaaggtgc gcaccatacg attgcgctct tccgcgtcct cgatttttgg atcgagcgga
     1501 tcggtgttct ccggcagatt gccttcctta accagccacg tcgtcgggtc ttcatacgcg
     1561 gcgatgatgc aatcaccttc gataccgagc cgcgcggcga ttccagcggt gagcttttcc
     1621 gcctgcacgc gcaaggcttc ctcagccgcc gcatcgcgcg gcatcgtctt gatttcttcc
     1681 gccgtgcgcg tcaccggcac cggcgcaatc agcgccggat ccttccagat cggcttgccg
     1741 tccttgcgcc aatagagcgc gaaggcccag cgcggcagcg tctcacccgg ataccatttg
     1801 ccctgcccgt aatggagcat accgcccggc gcgaaacggg tccgcagccg ccggatcaat
     1861 tcatcggcgc ggctgcgctt cgtcggaccg acagccgcgg tgttccactc gggcgcctcg
     1921 aaatcgtcga tcgatacgaa ggtcggctcg ccacccatgg taagcttcac gtcctgcgca
     1981 acgagatcgg catcgatctt cttgccgagc acattgagct cgtcccaggc ttcctcggag
     2041 aatggcagcg taatgcgcgg cgcctccgcg acgcgggtga cgttcatctc gaagccgaac
     2101 tcgacctgtg ccggctcgac agcgccggag atcggcgcgg cggaacggta atgcggcgtc
     2161 gcgcaaagtg ggatatgccc ttcgccgcaa aagaggcccg atgttgcgtc catgccgatc
     2221 cagccggcgc cgggaatata gacttcggcc caggcgtgga gatcggtaaa atcgtgttgg
     2281 gtgcctttgg gcccctcgat tggatcgaca tcggcgcgca actggatgag atagccggag
     2341 acaaagcggg cggcgaggcc gagacgccgc aaaatctgca cgagcagcca tgccgagtcg
     2401 cgacaggacc ccgaggcgag cgccaacgtc tcctccgggg tctggacgcc cggttccatg
     2461 cggatcacat agcgcaccgc gttctgcaga ttggcgttga gatcgacgag aaacgtgatc
     2521 gtgtggatcg gccctttcag cagagccgcg atatattcat caacccgcgg acccagcggt
     2581 tcggcttcga gataaggcgc cagctccttt ttgagctctg gcgcgtaagc gaaaggaaat
     2641 tgctcggcag acggctcgat gaaaaagtcg aacggattga tgacggcaag taccgcggtc
     2701 agatcgacct cgatcttcaa ttcactcgtc ttttccggaa agacgacccg ggccagccaa
     2761 ttgccgtgcg gatcctgctg ccaattgatg aaatggtccg gcggcgtgat cttgagcgaa
     2821 tagctcgcga tcttcgtgcg gcaatgcggc gccgggcgca ggcggattgt ctgcggccca
     2881 acttcgatcg ggcggtcata acgataatgc gtgacgtgat ggatagcggc ggaaatggtc
     2941 aaaggcgtat ccggtaaggt cgcgatgctc tagcctaaga acgtggcagg caccaagcaa
     3001 ctgctagagc gtcacacggc cgccgtatct cttcgcattc tgctcttccg ctaggcgtag
     3061 cggcgcgatg ctgagcattg accgatgtta ccggctgccg ccacaatcga cgaccataat
     3121 ttctcattgc caaggcgaag gcccatgaac tttcggcgca tactcaaggc gtctccgata
     3181 tttgtcgcgc tggccagttt ttacggcccc gcgctctccc aagcgccagc gtctcccaga
     3241 cccgaggacg cgctcgtcgc cgcctataat gcaaccggcg agcgcctgtt tcaggatctg
     3301 gcgcaggagc ccggcaatat cgtgctctcg ccctattcga tcggcagcgc cctctcgatg
     3361 gtgcttgccg gggcgcgtgg acagaccgcc gcggccacca gcgacacgcg tggcccgcac
     3421 ctttcagcta ccgatcttgc ggcggccaat gcaagcctga tcgccaatct caaagctgct
     3481 ggcggcaaag attctgtcac cctgaattcc gccaatgcac tgatcgaggt gcgacccggt
     3541 atcgtcagtg ctgattatcg cgccttgctg caaaaatcct acggcgccga agcgttctca
     3601 ggcgatctcg acgcgatcaa ccgatgggtt gcacagaaaa cctccggcaa gattccgcac
     3661 atcttggaca gcttgccgga tggaacgggg ttcgtcctcg cgaatgctgc ctacttcaaa
     3721 gcattctggc aacatccgtt cgataagcac gcgactgaag acggcacgtt tcacctgacc
     3781 gctttgaaac ccgtgcaagt gccgatgatg cacaacgaag accatttcgc gctcgtcaaa
     3841 ggcaacggct ataaagcgat ccgcctgccc tatcgatctg atggcgaacg tctcagcatg
     3901 attgttgtca tccctgacgc aatcaacggg cttggggcgc ttatcgccgg gcttggcccc
     3961 gatcagcttg ccaggctgct tgccgatttg aatactgcag actttgtgga cgtgcgattg
     4021 caagtgccgc gctttcacgc tgattttcaa gcgagcctcg ccgcgccgct gcaagagact
     4081 ggcatggcgg cggcgttcga tcttcagaaa gccgactttc gcggcataac tgacgggtcg
     4141 ctggctgcac agcaagttgc gatgggcgac gtcattcata gcgcggtggt cgatgtcaac
     4201 gaggagagtt cggaggccgc cgccgcgacc gtcgtgccga tgatcgcgac cgcgatggcg
     4261 cctcaatatg agccggtgcc agagcccttt atcgtggacc ggcctttcct gttcttcatc
     4321 gtggatcagc aaaccggcgc ggtcctgttc cagggaagaa ttgccgatcc gagaaaatag
     4381 cgtgcgctat ctcgcgctgg ctttggcgcg caacaaggca agcaagttcg cgccgccgat
     4441 gccgtccgcc ggcgtatcat gcgcctcaat ctgaaaagca tggatcgcat
//
LOCUS       DASWOG010000692         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_238173,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000692 DASWOG010000000
VERSION     DASWOG010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238173"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGB93_14160"
     CDS             <1..401
                     /locus_tag="VGB93_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0147261.1"
                     /translation="LVANCLAQSEALFRGRTQSEVESKLKAQGLGAAAIAALAPHKVF
                     SGNRPSSTFLYKSLTPRTLGRLVALYEHKVVVQAVVWNIDPFDQWGVELGKELALRLA
                     PLVADPQAATQDLDASTAGLLAHLRSLGRT"
     gene            459..1088
                     /locus_tag="VGB93_14165"
     CDS             459..1088
                     /locus_tag="VGB93_14165"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY0147262.1"
                     /translation="MHNRYNFIAGQSVERLAAISDGVFAVAMTLLVLDLHMPAAETIH
                     SEAGLWQAVVALAPRIFVYLMSFLTLGIFWVGQQTQLNQLERSDRDFTWVHLAFLFAV
                     SLTPFSTSLMAEFISYRIALIIYWANILLLGVILYAAWAFARRARLLKPDVTEEVDEA
                     VKRRIVGAQMLYAFGAALCLISTYWALGFILLVQLNYALAPRLRFPRRP"
     gene            complement(1170..1478)
                     /locus_tag="VGB93_14170"
     CDS             complement(1170..1478)
                     /locus_tag="VGB93_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY0147263.1"
                     /translation="MPSAPKKTATKAKAPAAKKPASAGSAFNKPLTPSKELAAVVGAA
                     PLPRTEVVSKVWEYIKKHKLQNPTNKREILADDKLEAVFGKKSVTMFEMNKYLAKHLK
                     "
     gene            complement(1710..2945)
                     /locus_tag="VGB93_14175"
     CDS             complement(1710..2945)
                     /locus_tag="VGB93_14175"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0147264.1"
                     /translation="MSDPKKIKRVVLAYSGGLDTSIILKWLQTTYQCEVVTFTADLGQ
                     GEELEPARQKALLLGIKPEHIFIEDLREEFVRDYVFPMFRANAQYEGLYLLGTSIARP
                     LIAKKQIEIARKVGADAVAHGATGKGNDQVRFELAYYALEPDIKVIAPWREWDLTSRT
                     ALLNFAEQNQIPIAKDKRGEAPFSVDANLLHVSSEGKVLEDPAQEVPEYVHSRTVNPE
                     DAPDTPTFIEIDFKSGDAIAVDGKDMSPATLLTHLNALGRVNGIGRLDLVENRFVGMK
                     SRGMYETPGGTILLAAHRGIEQITLDRGAAHLKDELMPKYAELIYNGFWFSPEREMLQ
                     ALIDKSQEFVTGKVRLKLYKGSVGIVGRSSPYSLYDQDLVTFEDGAVAYDHKDAAGFI
                     KLNALRLRTLARRKAKTTK"
     gene            3229..3918
                     /gene="rimP"
                     /locus_tag="VGB93_14180"
     CDS             3229..3918
                     /gene="rimP"
                     /locus_tag="VGB93_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0147265.1"
                     /translation="MDLSLQSTVDEKRFTSETGLAARIASVSQPVLADFGFRLVRVKL
                     SAQAGATIQIMAERPDGSMSVDDCETLSQALSPVLDVEDPIPEGYRLEISSPGLDRPL
                     VRASDFVRALGHEAKIEMAVPVGSRKRFRGVIESLEGDATEVKIRRTDAKPDEETEIV
                     LKLADIAEAKLMLTEDLIRASLRAAKQPEAESDATEEAPRRGPGRFAKGKEKPAPAPA
                     EKTPKAKKTKG"
     gene            3964..>4431
                     /locus_tag="VGB93_14185"
     CDS             3964..>4431
                     /locus_tag="VGB93_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY0147266.1"
                     /translation="MAVSANRLELLQIADAVAREKSIDRQIVLASMEDAMQKAARSRY
                     GQETEVRADINPKTGEVRFSRLLQVVEQIENDAKEITLTEAHKKNPAAQVGDWIAETL
                     PPFDFGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGEIVNGVVKRVEYGNVI"
BASE COUNT          856 a         1317 c         1342 g          916 t
ORIGIN      
        1 tgctcgtcgc taattgcctc gcccagagtg aggccttgtt tcgcggccgc acgcagtcgg
       61 aggtcgaatc aaaactgaaa gcgcaaggcc tcggcgcggc ggcgatcgcc gcgctcgccc
      121 cgcataaggt cttcagcggc aataggccat cgagcacgtt cctttataag agcctgacgc
      181 cacgcacgct cggccgcctc gttgcactat atgagcacaa ggtcgttgtg caggccgtgg
      241 tctggaacat cgatccgttt gaccaatggg gcgtcgaact cggcaaggaa ctcgcgctgc
      301 ggctcgcgcc gctcgtcgcc gatccgcaag ctgcaacgca agacctcgac gcctcgaccg
      361 ccggattgct ggcacatctg cgatccctcg gcaggacttg atggctgtag cggaaaagac
      421 caaaaccttc cgggcaacca aacctcggaa gatcagcctt gcataatcgc tacaatttca
      481 ttgccgggca aagcgtcgaa cggctcgcgg cgatcagcga tggtgtcttc gccgtcgcga
      541 tgacactgct tgtcctcgat ctccatatgc cggccgccga gacgatccac agcgaagctg
      601 ggctctggca ggctgtcgtc gcgcttgcac cgcgcatatt cgtctatctt atgagctttc
      661 tgacgctcgg cattttctgg gtcggtcagc agacccagct caatcagctc gaacgcagcg
      721 atcgcgattt cacctgggtg catctcgcgt ttttgttcgc cgtgtcgctc acaccgtttt
      781 cgacgtcgct gatggcggag ttcatctcgt accgcatcgc gctcatcatc tattgggcga
      841 atatcttgtt gctcggcgtg atcctctacg ctgcgtgggc gtttgcccgt cgcgcgcggc
      901 tgttgaagcc cgatgtcacc gaagaggtag atgaggccgt caaacgccgg atcgtcggcg
      961 cgcagatgct ctacgccttc ggcgcggcac tctgcctgat cagcacttat tgggcgctgg
     1021 gctttattct gcttgtgcag ttgaactacg cgctggcccc gcgcctgcga tttccgcggc
     1081 gtccgtaatc ggccttgcgc cgaatataag cgcgagcagc cctgctgtcg cagggttgct
     1141 tcgcttgccc gcaatgtttc gcggcgttct tatttcagat gtttggcgag atacttgttc
     1201 atctcgaaca tggtgacgga cttcttgccg aagacggctt cgagtttatc gtccgccagg
     1261 atctcgcgct tgttggtcgg gttctgcagc ttgtgcttct tgatgtattc ccagaccttg
     1321 ctgacgactt ccgtccgcgg cagcggcgct gcgccgacaa cggccgcgag ttccttggag
     1381 ggcgtcaacg gcttgttgaa ggcgctgccg gcagacgctg gtttcttcgc agccggggct
     1441 ttggctttgg tggcagtctt tttgggtgcg ctcggcatcg ctctctctcc aatcgaatcg
     1501 tacgcgggct catagcccta gtacctaaaa tcgctgccct cgcaaacgga ccgcaagggg
     1561 aattggcgat ttcactgcat ttattcaggg caattgtggt tcttcgcact gcgggagctg
     1621 gcaaaaggcc atgagggtgc cgcttgacgc tcctcccggc cgcgagccat gctaaaaaca
     1681 catggctccg ggccgcgaag gcggcgcggc tatttcgtgg ttttggcctt gcggcgcgcc
     1741 aaagtccgca gccgtagtgc attgagcttg atgaaacccg ctgcatcctt gtgatcatag
     1801 gcgaccgcgc cgtcctcgaa tgtcacgagg tcctgatcgt aaagcgaata gggactcgaa
     1861 cggccgacga tgccaaccga gcccttgtag agtttcagac gcactttgcc cgtgacgaat
     1921 tcctgactct tgtcgatcag cgcctgcagc atctcccgct cgggcgagaa ccagaagccg
     1981 ttgtagatga gctcggcata tttcggcatc agctcgtctt tcaaatgcgc ggcaccgcga
     2041 tcgagcgtga tctgttcaat gccgcgatgc gcggcgagca gaatggtgcc acccggcgtt
     2101 tcatacatgc cacgcgattt catgccgacg aaacggttct cgacaagatc gagccgaccg
     2161 atgccattga cgcggccgag cgcgttcaga tgcgtgagca gcgttgccgg cgacatgtcc
     2221 ttgccgtcga cggcgattgc gtcgccggat ttgaaatcga tctcgatgaa agtcggggtg
     2281 tccggcgcat cctcaggatt gacggtgcgc gaatggacat attccggcac ttcctgcgcc
     2341 gggtcttcga gcactttgcc ttccgacgaa acgtgcaaca ggtttgcgtc cacggagaac
     2401 ggcgcttcgc cgcgtttgtc cttggcgatc ggtatctgat tctgctcggc gaaattaagc
     2461 agtgccgtgc gcgatgtcag atcccattcg cgccacggcg cgatgacctt gatgtccggc
     2521 tcaagggcgt aataagcaag ctcgaagcgc acctgatcgt tgcccttgcc ggttgcgcca
     2581 tgcgccaccg catcggcgcc gaccttacgg gcaatctcga tctgtttctt ggcgatcagc
     2641 ggccgcgcga tcgacgtgcc gagcagataa agtccctcgt attgcgcatt ggcgcggaac
     2701 atcgggaaga cgtaatcgcg gacgaattct tcgcgcagat cttcgatgaa aatatgctcg
     2761 ggcttaatgc ccagcagaag cgccttctgc cgcgcgggct caagttcctc gccctggccg
     2821 aggtcggcgg tgaaggtgac gacttcgcat tgataagtcg tctgcagcca tttcaggatg
     2881 atcgacgtat cgaggccgcc ggaataagca aggacgacgc gtttgatctt tttgggatcg
     2941 gacattttct tctcgaaaaa gggatttgaa gcggcgcgac tatagagagc acgcctttgg
     3001 gcgcaagcga tcgttgcctg cgccagcgtt tgcgaccttg caaaccggtc cgcgaaaatg
     3061 tatatctgcg gcatcgtaat ttctcatagc ggtcatcgtc cggtgatcga gttttgagag
     3121 tgggccgccc gccggacccg ctctttttta ttgccctctg ccggacgggc catggcccat
     3181 gaaaagttac taaaaatcaa tatgttaact ccatcggccg cgccgaaaat ggacttgagc
     3241 ttgcagagca ccgtggacga aaagcgtttc acctcggaga cgggcctcgc cgcgcggatt
     3301 gcctcggtga gccagccggt gctggccgat ttcggcttcc gcctcgtgcg ggttaaactt
     3361 tcggcccagg ccggtgcgac catccagatc atggcggagc ggccggacgg cagcatgtcg
     3421 gtcgacgatt gcgagacgct gagccaggct ttgtcgccgg tcctcgatgt cgaggatccg
     3481 attcccgaag gctataggct ggagatttct tcacccggcc tcgaccgtcc gctggtgcgc
     3541 gcctcggatt tcgtccgtgc tctcgggcat gaggccaaga tcgaaatggc ggtgccggtc
     3601 ggcagccgca agcgcttccg tggtgtcatc gaaagcctcg aaggcgacgc cacagaggtc
     3661 aaaatccggc gcaccgatgc gaaaccggac gaagagaccg aaatcgtgct gaagctcgcc
     3721 gacatcgcgg aggccaagct gatgctgacc gaggatttga tccgcgcgtc gctgcgcgcg
     3781 gcaaaacagc ccgaagcgga aagcgatgcg accgaggaag cgccccggcg cggtcccggc
     3841 cgttttgcca agggaaaaga aaagcccgcg ccagcgccag cggaaaagac accgaaagcg
     3901 aagaagacca aaggctaacc cgcgagggcg ccaaggcgcc gtcggatttc aaggagactt
     3961 ttcatggccg tcagtgccaa caggctcgaa cttctgcaga tcgccgacgc ggtcgcgcgc
     4021 gaaaaatcga tcgaccggca gattgtgctg gcctcgatgg aggacgcgat gcagaaggcc
     4081 gcccgctccc gctacgggca ggagacggaa gtgcgcgccg acatcaaccc gaagaccggt
     4141 gaggtgcgct tctcgcgcct gttgcaggtg gtcgagcaga tcgagaacga cgccaaggaa
     4201 attacgctta ccgaggcgca caagaaaaat cctgccgcgc aggtcggtga ctggatcgcc
     4261 gagaccttgc cgccgttcga cttcggccgc atcgccgcgc aatcagcgaa acaggtgatc
     4321 gtgcagaagg tgcgcgaggc cgagcgcgac cggcaatatc aggaattcaa ggatcgcatc
     4381 ggcgagatcg tcaacggcgt cgtcaagcga gtcgaatacg gcaacgtcat c
//
LOCUS       DASWOG010000693         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_71876,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000693 DASWOG010000000
VERSION     DASWOG010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71876"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGB93_14190"
     CDS             <1..492
                     /locus_tag="VGB93_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0147267.1"
                     /translation="PRVLAARLAAEKAEGAAKIAAQRPELDGAFLVAADTVVAVGRRI
                     LPKCEVVDEATQCLRLLSGRVHRVYTGVTLITPKGGRRRRLVESRVRFKRFSTDEIEA
                     YLASGEWRGKAGGYAIQGLAGAFVVKLVGSYTGVVGLPLYETLSLLAGEGFPAHFFWL
                     NRV"
     gene            530..754
                     /gene="yacG"
                     /locus_tag="VGB93_14195"
     CDS             530..754
                     /gene="yacG"
                     /locus_tag="VGB93_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY0147268.1"
                     /translation="MQSKAHEKHDGAPQGRPCPICGKPASAADRPFCSARCADVDLLH
                     WLKGAYALPALPDDDDTDDSALKEASPEQD"
     gene            788..1576
                     /locus_tag="VGB93_14200"
     CDS             788..1576
                     /locus_tag="VGB93_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0147269.1"
                     /translation="MFFVVAKLFWALAEPFNFLLILGLIGVVLSFSPWRCWGHRLCVI
                     SLTLFAILTFSPLGAFLLRPIEDRFPLPPSNIAAPTGIIVLGGGLDEDLTATRHEPTE
                     VGERVTMGAVLARRFPKARLIFTGGSSSLRPSGADEATGVRELWIELGVDPARMTFET
                     KSRNTWENAIFARPLARPQPGDTFLLVTAAWHVPRAMGVFRRLGYHVIAYPTDYETRG
                     DAGDYGWFHLGTGQLGMFQAGLHEWIGLTVYHFTGKTSAWFPVP"
     gene            1695..2531
                     /locus_tag="VGB93_14205"
     CDS             1695..2531
                     /locus_tag="VGB93_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0147270.1"
                     /translation="MRLSWLAIAVVPFALSTAPAFAVDPLDIQHCQQQGDADLAISGC
                     GKIAADKSIDVHDRAVAAFNLGIGYYAKNDLDNAIKAWTDAVSFEPAYAHAYNNLAKA
                     YLAKTDFGSAIKNYDQVIKLDPKHGLAYKGRGIAEFFNGEKEKAKVDFEQAVALDSSD
                     AYSLLWLELTKRRLGAKPSPDLAKATTQVNMTSWPAPLLLLYAGQMTPHDVVLAAQNV
                     DLKVSKARMCDVGFYAGEFSLAEGEKSDAIELFNHSLDVCFNSVDEKTAATAELKALG
                     QN"
     gene            2545..3198
                     /locus_tag="VGB93_14210"
     CDS             2545..3198
                     /locus_tag="VGB93_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0147271.1"
                     /translation="MSLTLFIANKAYSSWSLRPFILMRHFGVAFDEIAIALGQETTRA
                     DILKYSPSARCPALIDGDLVIWDSLAIIEYVAEKFPHLPVWPRERAARAVARSLAAEM
                     HSGFQPLRAHLPMNMRRAVKRRDLTPEVLADVARLEIAWSDARERFGAGGDFLFGDFC
                     AADAMFAPVVSRLYIYDAPVSAASRAYMEAVMALPAYQDWRKGAAAETWRIAKYEEV"
     gene            3256..3951
                     /locus_tag="VGB93_14215"
     CDS             3256..3951
                     /locus_tag="VGB93_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HEY0147272.1"
                     /translation="MHYLPLSLPFFFMLGGILVLLVIWVEIRALTFAYQRAGISSPAA
                     LFMLFASWVGGYVNIPLFWLHGRPVMSDQQVDFFGMQYVVPTADWQGTVLAVNVGGCV
                     IPVLLSLYILAKYDLWVLGAVGTAIVAVITHMMATPVHGVGIALPIFVPPIITAIVAA
                     ILSRTYAGPLAYVSGSLGVLIGADLSNLNKVADLGAPVASIGGAGTFDGIFLTGVLAV
                     LLASLTAPRYQPA"
     gene            complement(3957..>4616)
                     /locus_tag="VGB93_14220"
     CDS             complement(3957..>4616)
                     /locus_tag="VGB93_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY0147273.1"
                     /translation="MDAGGKDGTISHVMRCMNPQGVSVARFETPTPDEKAHDFLWRVH
                     QKAPRYGQVGVFNRSHYEEVLITRVHKLIDKDTYKSRYRQICDFEAGLTENGTHIIKF
                     FLHISKEEQLARFAQRLDDPQRNWKISESDYTERDYWDDYREAYEDALAATSTEDAPW
                     FVIPSNHKWFRNLAISQIIAQTLTDLGMKYPEPSVDRAKIRRDYHTAVAEADGKKKRK
                     N"
BASE COUNT          780 a         1354 c         1440 g         1042 t
ORIGIN      
        1 ccgcgtgtcc tcgccgcgcg acttgcggcc gaaaaggcgg agggtgcagc gaagatcgca
       61 gcccagcgcc ccgaactcga tggcgccttt ctggttgcgg ccgatacggt cgtcgcggtc
      121 gggcgccgta ttctgccgaa atgcgaggtc gtcgatgagg caacgcaatg cctccgactg
      181 ctctcgggcc gcgttcaccg tgtctatact ggcgtgacgc tgatcacgcc gaagggcggc
      241 cggcgacgcc ggctggtcga gtcgcgcgtc cgcttcaaac gcttctccac cgacgagata
      301 gaggcctatc tcgcctccgg ggaatggcgc ggcaaagccg ggggctatgc gatccaaggt
      361 ctcgctggcg cctttgtggt aaagctcgtt gggtcctata ccggcgtggt cggcctgccg
      421 ctttatgaga cgctgtcgtt acttgccggg gaagggttcc cggcgcattt tttctggttg
      481 aatcgtgtct agtcgcgaag tcttcctcgg agggcatcct aggagcatca tgcagtccaa
      541 ggctcacgaa aagcacgacg gcgcacctca gggccgcccg tgcccgattt gcggcaagcc
      601 ggcgagcgct gcggatcggc cgttctgctc ggcccgttgc gccgacgttg acctgcttca
      661 ttggctcaaa ggcgcgtacg cgctgccagc gctgccggat gatgacgaca cggacgattc
      721 cgcgctaaag gaagcctcgc cggagcagga ttgatcccgg tgccgaggtt cagtccgcgg
      781 gagcgccatg tttttcgttg tcgccaagtt gttttgggcg ctcgctgagc cgttcaattt
      841 cctgctgatc ctgggcttga tcggggtggt gctgagtttc agcccgtggc gctgttgggg
      901 tcatcggctc tgcgtgatct cgctcactct tttcgcgatc ctgacgtttt cgccgctcgg
      961 cgcgtttctg ctgcggccga tcgaggatcg ttttccactg ccgccttcga atatcgccgc
     1021 gccgaccggc atcatcgttc tcggcggcgg cctcgacgag gatctgacgg cgacccggca
     1081 cgagccgacg gaggtgggcg agcgcgtgac gatgggcgcg gtcctcgcgc gccggtttcc
     1141 caaggcgcgc ctgattttta ccggcggctc ctcaagcctg cgcccgagtg gcgcggatga
     1201 agcgacgggc gtgcgtgaac tctggataga attgggcgtc gacccggcgc gcatgacctt
     1261 cgagacgaaa tcgcgcaata cctgggagaa cgcgattttc gcgcgcccgc tggcgcggcc
     1321 gcagccgggc gacacgttcc ttctggtcac tgcggcgtgg cacgtgccgc gcgcgatggg
     1381 cgtgtttcgc aggctcggct atcacgtgat cgcttacccg accgattacg agacccgtgg
     1441 tgatgctggc gattatggct ggttccatct cggcaccgga cagctcggga tgttccaggc
     1501 cggcctgcac gaatggatcg gcctgaccgt ctatcatttc acaggtaaga cgagtgcctg
     1561 gttcccggtg ccgtgacgca gggtgaaatc agttgcgcaa aatgccgtaa cccaataaat
     1621 ccgcaatgat tttcgacatc aaacacaata agcgcgcggt gcagccggcg caacagagca
     1681 gggaaggaag ttttatgcgt ctttcgtggt tggcgatcgc cgtcgttccg ttcgcgcttt
     1741 cgacagcgcc cgcttttgcc gtcgatccgc tcgacattca gcattgccaa cagcagggcg
     1801 atgcggatct ggcgatcagt ggttgcggca agatcgctgc ggataagagc atcgatgttc
     1861 acgaccgcgc cgtcgccgcc ttcaacctcg gcatcggcta ttatgcgaaa aatgatttgg
     1921 acaatgcgat caaggcctgg accgacgccg tttcgttcga gccagcttac gctcatgcct
     1981 acaacaatct ggccaaagcc tatttggcaa agaccgattt tggttcggcg atcaagaatt
     2041 acgatcaggt gatcaagctc gatccgaaac atggactggc ttacaagggc cgtggtattg
     2101 ccgaattttt caacggcgaa aaggaaaagg ccaaagtcga ttttgagcag gccgtggcgc
     2161 tcgattcgtc cgatgcttat tcgctactgt ggctcgaact gaccaagcgc cggctcggtg
     2221 ccaagccctc gccggatctc gccaaagcca cgacgcaagt gaacatgacg tcatggccgg
     2281 cgccgctgct tctgctctat gccggccaga tgacgccgca cgatgttgtt ctcgcagcgc
     2341 agaacgtcga tctcaaggtt tccaaagcgc gcatgtgcga tgtcggcttc tacgccggcg
     2401 agttttcgct cgccgaaggt gaaaagagcg acgcgatcga gttgttcaat cattcgctcg
     2461 atgtttgctt caattcggtc gatgagaaga cggctgcaac ggcagagctc aaagccctcg
     2521 ggcagaacta ggaacgaggc gcgcgtgtcg ctcacgcttt tcatcgccaa caaggcttat
     2581 tcgtcctggt cgctgcggcc gttcatcctc atgcgacatt tcggggtcgc attcgacgaa
     2641 atcgcgattg ccctggggca ggagacgacg cgggcggata ttctcaaata ttcgccgtcg
     2701 gcgcggtgtc cggccttgat cgacggcgat ctcgtgatct gggattccct cgccatcatc
     2761 gaatatgtgg ccgagaaatt tccccatctg ccggtctggc cgcgtgaacg ggcggcacgc
     2821 gctgtcgccc gctcactggc ggctgaaatg cattcgggtt tccagccgct gcgtgcccat
     2881 ttgccgatga acatgcgccg cgcggtgaaa cgtcgcgatc tcacgccgga ggtcctggcg
     2941 gatgttgcgc gtcttgaaat tgcatggtcc gatgcgcgtg aaaggttcgg cgcgggcggg
     3001 gactttttgt tcggcgattt ctgtgcggca gacgcgatgt tcgcgccggt ggtgagccgg
     3061 ctctatattt acgatgcgcc cgtcagcgcc gcgagccggg catatatgga ggcggtcatg
     3121 gcgttgcctg cctatcagga ttggcgcaaa ggcgctgcgg cggagacttg gcgcatcgcc
     3181 aaatacgagg aagtctaagc gtcgggcggt ctaatccggc gcaattgaaa atgcgacgca
     3241 cggagaccgg acgaaatgca ttatttgccg ctgtcgctgc cgttcttttt catgctcggc
     3301 ggcattcttg tcttgctcgt catctgggtc gaaattcggg cgctgacctt tgcctatcag
     3361 cgtgctggga tcagttcgcc cgcggccttg ttcatgctgt tcgcctcctg ggttggcggc
     3421 tacgtgaata ttcccttgtt ttggctgcat ggccgcccgg tcatgtcgga tcagcaggtt
     3481 gatttctttg gcatgcagta tgttgtgccg accgccgact ggcagggaac ggtgctcgcc
     3541 gtcaatgtcg gcggttgcgt gattcccgtt ctgctctcgc tctatatcct ggcgaaatac
     3601 gatctctggg tgctcggcgc ggtcgggacc gcaattgtcg cggtcatcac gcatatgatg
     3661 gcaacgccgg tgcatggggt cggtattgcg ttgccgatct tcgtgccgcc gatcatcacg
     3721 gcgatcgtcg ccgccattct ctcgcgcact tatgccggcc cgcttgccta tgtcagcggc
     3781 agcctcggcg tgctgattgg cgcggatctc tcgaatctga acaaagttgc ggaccttggc
     3841 gcgccggtcg cgtcgatcgg cggggccggg acgttcgacg gcattttcct gaccggtgtg
     3901 ctggccgtgc tgttggcgag cctcaccgcg ccacgctatc agcccgcttg aggccgttag
     3961 ttcttgcgct tcttcttccc gtccgcctcc gcgaccgccg tgtgatagtc gcggcggatt
     4021 ttggcacgat cgacgctggg ctctggatat ttcatgccca aatccgtcag cgtctgcgcg
     4081 atgatctgag agattgccaa attgcggaac catttgtggt tggagggaat gacgaaccaa
     4141 ggcgcatctt cggtactcgt cgccgcgagc gcgtcctcgt aggcctccct ataatcgtcc
     4201 caatagtctc gttctgtata atctgactcg ctgattttcc agttacgctg cggatcgtcg
     4261 agccgttgcg cgaaccgcgc gagctgttcc tccttgctga tatgcaggaa gaatttgatg
     4321 atatgcgtgc cgttctcggt gagcccggcc tcgaaatcgc aaatctggcg atagcgcgat
     4381 ttgtaagtat ccttgtcgat cagcttgtgg acgcgggtga tcagcacctc ttcgtaatgc
     4441 gaccggttga agacgccgac ctgcccatat cgcggcgctt tctgatggac gcgccagaga
     4501 aagtcatggg ctttttcgtc cggcgtcggg gtctcgaacc gggcgacgct gacgccttgt
     4561 gggttcatgc atcgcatgac atgcgaaatg gtcccatcct tgccgccggc atccat
//
LOCUS       DASWOG010000694         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_218464,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000694 DASWOG010000000
VERSION     DASWOG010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218464"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..416
                     /locus_tag="VGB93_14225"
     CDS             213..416
                     /locus_tag="VGB93_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147274.1"
                     /translation="MTKSNPSLAAEAAKRDAEDKAKAATKSKPEDSELDEALEESFPA
                     SDPPSQTQPETGVGTPAEPRKKR"
     gene            complement(548..907)
                     /locus_tag="VGB93_14230"
     CDS             complement(548..907)
                     /locus_tag="VGB93_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147275.1"
                     /translation="MKKLRIRIVEDDVVARIALESVLTDITSVAVIAKRSVAGASEVL
                     NERFDIAFIDVSVADGDTFGLASALIAHAVPVVFMGDKSLTLPTAWRSFAFISKPPRA
                     AAVRQALAQAEEVQLVA"
     gene            complement(1489..2184)
                     /locus_tag="VGB93_14235"
     CDS             complement(1489..2184)
                     /locus_tag="VGB93_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0147276.1"
                     /translation="MLERFLIVDDHPLFREALQNAIHLAFPVAETLEASSIDEARKIL
                     EAEENIDLLLLDLSMPGTQGFEGLLALRRQRPRLPVVIVSGLEDSRIVAEALACGVAG
                     FIPKSVRKPELASAIRNVMNGMVYVPEWYAPDQTPPVNDSVGVAARLKTLTPQQLRVL
                     EMLRQGKLNKQIAHELSVGETTVKAHITEIFRKLNVHSRTMAAMQLAKVDFAAVLADA
                     GVSEKRPRRTTDA"
     gene            complement(2366..3730)
                     /locus_tag="VGB93_14240"
     CDS             complement(2366..3730)
                     /locus_tag="VGB93_14240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0147277.1"
                     /translation="MTLANLNQIDENFPQAESAERRIEKLTKINRALMRRVERAMEQP
                     ENAASLFQAVIALGSEARSRTEELKSTLVELERSNLELKEARDGAERSNRIKTGFFTA
                     VGHDVLQPLQAARLTLSALAEIEEGQPGGPLVGQVDQALSIVEDLLRTMLDLSRLETG
                     ALRPKIQPVDLNEIFNSIVSGLLPLAVDKGITLRFRRTRFWVMSDPLMLRRIVQNLAA
                     NAVRYTEIGHVLICARPRGREVCVAVYDTGPGIADAEREVIFQEFERGEASERGKSLG
                     FGLGLSIVRRMSTALKHDIFLRSYEGRGSCFGLRVPRTLALARQPGDPIQSPIRQMNL
                     SARAVIIENDKAIAAGMITLLRQWGCETKAVRNFSEASDLAGGPAPDIVLADYHLDNG
                     EVGLKSVRLLRKVFGEGLPAVVLTADRTPDVAEAVKEARCELLLKPVKPAEMRSLITH
                     LLRR"
     gene            complement(3851..>4942)
                     /locus_tag="VGB93_14245"
     CDS             complement(3851..>4942)
                     /locus_tag="VGB93_14245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0147278.1"
                     /translation="LALVCDSTNILRDGISPSEAEVALALTEVIGGATARVVVTTFAS
                     NVARIRAVALAAKAAGRRVVLLGRAMERVVNVAREYGYLEGVDPFLSGEAFATLRREE
                     IVVLATGSQGEPRAAMARIAVDDHPIAKLSPGDMVIFSSRTIPGNEREVGRIVNNLSG
                     QGVEVVTDRNALIHASGHPRRGEVAQFYEWVRPRIIVPAHGEELHLSEHAVFAAERGI
                     ETVVKARNGDLVVLAPGAPAIIDEVPHGQLARDGSILVPLEGESIKARQRLAFAGIVT
                     IALAIDRKGEMAGVPDVVLTGLPEKTASGVALDEIVDAALFQTFESLPRPKRRDANVV
                     SVAIEKAVRAAIGVVWNKRPAVHVLVIEV"
BASE COUNT         1068 a         1528 c         1386 g          960 t
ORIGIN      
        1 cttgcgggtc ctcatggact ttagcggggc gaggtaccgg cttatgttgg aagtttgggc
       61 gagtcattcc gcaatgagac ttaggatgtg ccttccgatc agagatgcga ttacggagtt
      121 agcactggga aatcacgagc gcacggctag atgcacgcgc atcgacgcaa ccttaagttt
      181 agcccgacgt tatcaaagcg aggaggacga ttatgaccaa atccaaccca tctctcgccg
      241 cggaagccgc taaacgcgac gctgaggaca aggcgaaagc tgctaccaag tcaaagccgg
      301 aggacagcga actcgacgag gcgttggaag aatccttccc cgcaagcgac ccgccatcgc
      361 agacacagcc agagaccggt gttggcacgc cggctgagcc gcgaaaaaag cgctagcggc
      421 ggccggcggg gctataaggc gattgtcgaa gaaccgccaa ccccgcggat cggcggcttt
      481 acagcgttga attccgagaa agacctctgc gcaaaattct tcttcgctct ggattgggga
      541 gacccggcta cgccacaagt tgaacttctt cagcctgagc caaggcttgg cgaaccgccg
      601 cggcgcgagg cggcttggag atgaaggcaa aactacgcca tgcagtcggc aaagttaatg
      661 atttgtcgcc catgaaaacg accggcacag cgtgcgcgat caaagcagac gccaacccaa
      721 acgtgtcacc atccgcgacg ctaacatcaa taaaagcgat atcgaatcgt tcgttcagga
      781 cctcgctcgc gccagccaca gagcgtttgg caatcaccgc aacggaggtg atgtccgtca
      841 gcacgctttc cagcgctatc ctcgcgacga catcgtcttc cacaatgcga atgcgaagct
      901 ttttcatgcc cataacctcc gcgttgatag cggttaaacc aagcggagcc taaaaagttt
      961 cgcggcgtga aaacgccggc ttgtgcgcaa tggcacaaat caagtggatc cgcttcgccc
     1021 atcgctcagt ttggaacaat aggcgttcca tcctgaacac aagctgaggg gctgatgagt
     1081 atgtcgttca tgtctcaaac ctcagtctgc cgacacaaat gcgccgtctt tgcccttcaa
     1141 gtgccagtct tcatcaattt gacacgaaac ttgcaggcct gcgctcgcaa tggccaaagg
     1201 ataaatgatg tcgttgaaga ccgatgcaat gattaaaacc aatattcaga atttcgctgg
     1261 cgctgcgctt ttgcttattt ctgcctgcgc tgtttcatcg tcggcccaag ctttcacttg
     1321 cgatgatgtg cgccatctga catttgccga acagaattat tatgcaaaac tgctccacat
     1381 cagcaccgtc gagcggcgcc agatttgggc ggcatgctat cgcaactatc gtccgggtct
     1441 acaggcgcaa ttgattcggc gctaagcttc ccgaatgtag ccgacacctc aagcgtccgt
     1501 cgtgcgccgc ggtcgttttt ctgacacccc ggcgtcggcc agcacagccg caaaatccac
     1561 tttcgctaat tgcatcgccg ccatcgtgcg gctgtgcacg ttgagtttgc ggaagatttc
     1621 ggtgatatgc gccttcaccg tcgtctcacc gacgctgagc tcgtgcgcga tctgcttgtt
     1681 gagcttgcct tgtcggagca tttcaagcac gcgcaactgc tgcggtgtga gagtcttcag
     1741 acgcgcggca accccgacgc tatcattgac gggcggcgtc tgatcggggg cataccattc
     1801 cggtacgtaa accataccat tcatgacatt acggatggcg gaggccagct cgggcttgcg
     1861 gaccgatttc ggaataaagc ccgcgacgcc gcaggcgagc gcctcggcaa cgatacggct
     1921 atcttcaaga cccgagacga tcacgaccgg cagccgcgga cgctgacgcc gcaaggccaa
     1981 aagcccctca aagccctgcg tgcctggcat cgacagatcg agcagtagaa ggtcgatatt
     2041 ctcttccgct tcgagaattt tccgcgcttc gtcgattgag ctggcttcga gggtttccgc
     2101 caccggaaat gcaagatgaa tcgcgttctg gagcgcctcg cggaacagcg ggtgatcgtc
     2161 gacgattaaa aaccgctcca gcatcgtgcg tcccgaccct ttagcctccg aaaatgtaac
     2221 atgttgcgca ggcattgaaa tcggctttct tcgagagcac ggcacagagg cgaatcctag
     2281 ccgccgccgc ggacgtcgcc atcatagaaa gcacggccaa ccgcgccatt ggactttcgg
     2341 ccattcgcac gagcgcgacg aggctttacc tgcgcaaaag atgggtgatg agtgaccgca
     2401 tctccgccgg cttcaccggt ttcaggagca actcgcagcg ggcctcctta acagcctcgg
     2461 cgacgtccgg tgtccggtct gccgtcagga cgacagcagg caagccttca ccgaagactt
     2521 tgcgcagcag ccgcacggac ttcagcccga cctcgccatt gtcgagatga tagtcggcga
     2581 gaactatatc gggggccggc ccaccggcga gatcgctggc ctcgctaaag ttacgaaccg
     2641 ccttcgtttc acatccccat tgccgcagca aggtgatcat cccggccgcg atggctttat
     2701 cgttttcgat aatgaccgcg cgggctgaca aattcatttg ccggataggc gattggatgg
     2761 ggtccccagg ctggcgcgcc agcgccaagg tcctcggaac ccgcaggcca aagcacgagc
     2821 cgcgtccctc ataagagcgc aggaaaatgt cgtgcttaag tgccgtcgac attcggcgca
     2881 cgatcgaaag gccaagcccg aagccaaggc ttttgccccg ctcggaagct tcgccgcgct
     2941 cgaactcctg gaaaatcact tcgcgctcgg catcggcgat gcctgggccc gtgtcataca
     3001 ccgcaacgca gacctcacgt ccgcgcggcc gcgcacaaat cagcacatgg ccgatttccg
     3061 tatagcgaac cgcgttggcg gcaaggttct ggacaatacg ccgcaacatc agtggatctg
     3121 acatgaccca gaaacgtgtc cgccggaaac gcagcgtgat gcctttatca accgccaaag
     3181 gcaacaagcc gctgacgatc gaattgaaaa tctcattgag gtcgacgggt tggattttcg
     3241 gccgtagcgc gccggtttca agccgcgaca gatcaagcat cgtcctcagc aaatcttcga
     3301 cgatcgacaa ggcttgatcg acctgcccga cgagcggacc gccgggctgg ccttcttcta
     3361 tttcagcaag cgccgacaat gtgagccgcg ccgcctgcag cggctgcagc acgtcatggc
     3421 caactgcagt gaaaaaaccc gtcttgatcc gattcgagcg ttcggcgccg tcgcgcgcct
     3481 ccttcaactc caggttggat cgctccaatt ctacgagcgt tgacttcaac tcttctgtgc
     3541 gggatcttgc ttcgctgcca agggcgatca ccgcttgaaa aagcgaggcc gcattctccg
     3601 gctgttccat cgcgcgttcg acgcgtcgca tgagcgcgcg attgattttc gtaagctttt
     3661 caatacgccg ctcggcgctc tcggcttgcg ggaagttttc gtcaatctgg ttgagattgg
     3721 ccagcgtcat cgtctctcat tccgggccgg acgcggcgcg gggcgcgtcc agcttggccg
     3781 gattggcggg gcgccagcgt caaagctcta aaactttaca aactgacgca ggggttgtcc
     3841 atgcgtaaga ttacacctcg ataaccaaga cgtggaccgc cggcctcttg ttccagacga
     3901 cgccgatagc cgcacgcacc gccttttcga tcgcgacaga gacgacattg gcgtcgcggc
     3961 gcttcggccg gggcaaagac tcgaaggtct ggaacagggc tgcatcgaca atttcgtcga
     4021 gagcgactcc cgacgcggtc ttctcgggca ggccggtcaa aaccacatcc ggaacgccag
     4081 ccatttcacc ttttcgatcg atcgccaggg caatcgtcac aatgcccgcg aatgcgagcc
     4141 gctggcgcgc cttgatcgac tctccctcca acggcacgag gatgctgccg tcgcgggcaa
     4201 gctggccgtg cgggacctca tcgatgatgg caggcgcgcc gggtgcgagc acgaccagat
     4261 cgccgttgcg ggccttcaca acagtttcga tgccgcgctc tgccgcgaac acagcatgtt
     4321 cgctaagatg cagctcctcg ccgtgtgccg gcacgatgat cctcggcctg acccattcgt
     4381 aaaattgcgc gacttcaccg cggcgcggat gacccgaagc atgaatcaac gcgttgcggt
     4441 cagtgaccac ttcgacgccc tgcccgctca gattgttcac gatgcggccg acctcgcgct
     4501 cattgcccgg aatggtccgc gatgagaaaa tcaccatgtc gcccggtgac agtttggcga
     4561 tcggatgatc atcgacagcg atccgcgcca tcgcagcgcg tggctcgccc tggcttcctg
     4621 tcgcgaggac gacgatttcc tcccgccgca gcgtcgcaaa agcctcgccc gatagaaaag
     4681 ggtcgacgcc ttcaagatag ccgtactcac gcgcaacatt aacaacgcgc tccatggcgc
     4741 gcccgaggag gacgacccga cgtcccgccg cctttgcggc caacgccact gcgcggatac
     4801 gcgcgacatt cgaggcaaag gtcgtgacga cgacacgcgc cgtcgcgcca ccgatcacct
     4861 ctgtcagtgc cagtgccact tcagcctctg aaggactgat accgtcgcgc agaatattgg
     4921 tggaatcgca gacgagcgcg ag
//
LOCUS       DASWOG010000695         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_71890,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000695 DASWOG010000000
VERSION     DASWOG010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71890"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1086
                     /locus_tag="VGB93_14250"
     CDS             55..1086
                     /locus_tag="VGB93_14250"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0147279.1"
                     /translation="MLLRTNIIEAIGHTPLIKLQHASKATGCEIYGKAEFMNPGGSIK
                     DRAALAIVRDAEARGALKQGGMIVEGTAGNTGIGLALVANALGYRTVIVIPDTQSQEK
                     KDMLRLQGAELIEVPAVAYSDPNNYVKLSGRLAEKLAHDHIQGAIWANQFDNVANRQG
                     HIETTGPEIWADTDGKIDGFVCSVGTGGTLAGVAIALKAKNPKIKIALADPLGVALYE
                     YYTNGVLKSEGSSITEGIGQGRITANLEDAPVDIAYQITDTEALPVLFDLAAHEGLLL
                     GGSSAISVVGALRLAKELGPGHTIVTILADSGARYLSKLYNPEFLREKHLPVPPWLER
                     HVEIKPPYL"
     gene            1097..1747
                     /locus_tag="VGB93_14255"
     CDS             1097..1747
                     /locus_tag="VGB93_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0147280.1"
                     /translation="MMSDNLFVSTEWLAAHLGEPDLVIIDGTFLLPSEGRNAREEYRA
                     GHIPGTVFFDIDEIADHSIDLPHMLPTPEAFAKAAGALGINDMSRIVVYDTSNFQGGA
                     RVWWTLRLYGAKDVKLLAGGLARWKAEGRPLEQGEARPSPQTFNVTFDAAGVAGFEDV
                     RKIMQDHSREIVDARPAARFRGQTPEPRPGLSSGHIPGSFSVPSRDLVEDGEMKSP"
     gene            1793..1939
                     /locus_tag="VGB93_14260"
                     /pseudo
     CDS             1793..1939
                     /locus_tag="VGB93_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
BASE COUNT          474 a          696 c          674 g          456 t
ORIGIN      
        1 cagcccgcgt ggcgagcgtt tatatagccc acgaatttac tcaggaatgg aaatatgctg
       61 ctccggacaa acattatcga ggctatcggc cacacgccgc tgatcaaatt gcagcacgcc
      121 tcgaaggcca ccggctgcga gatttacggc aaggccgagt ttatgaatcc gggcggctcc
      181 atcaaggatc gcgccgcgct ggcgatcgtt cgtgacgccg aggcgcgcgg cgcattgaaa
      241 cagggtggca tgattgtcga gggaacagcc ggcaacaccg gcattggcct cgcccttgtc
      301 gccaacgcgc tcggctatcg caccgtgatt gtgattccgg acacgcagag ccaggaaaag
      361 aaggacatgc tgcgtttgca gggcgcggaa cttattgaag tcccggccgt cgcttattct
      421 gatcccaaca attacgtgaa actttccggc cgcctcgctg aaaagcttgc gcacgatcat
      481 atccagggcg cgatttgggc caatcaattc gataatgtgg caaaccggca aggccatatc
      541 gaaacaaccg gcccggaaat ctgggcagat acagatggca aaatcgacgg ctttgtctgc
      601 tccgtcggaa cgggcggcac gcttgccggc gtcgcaatcg cactgaaggc taaaaatccg
      661 aagatcaaga ttgcactggc agatccgctc ggcgtcgcgc tttacgaata ttacaccaac
      721 ggcgttctga aatcagaagg ctcgtcgatc accgaaggca tcggccaggg acgcatcacc
      781 gccaatctcg aagacgcgcc ggtcgacatc gcgtatcaga tcactgacac ggaggccctg
      841 cccgttctgt tcgaccttgc tgcgcatgaa ggtctgctgc tcggcggttc gtctgcgatc
      901 agcgtcgtcg gtgcattgcg actcgcgaaa gaactcggac cgggccatac catcgtcacg
      961 atcctcgccg attccggtgc gcgctatctc tcgaagctct acaatccaga gtttctgcgc
     1021 gagaagcatc ttcccgtgcc gccatggctc gaacgccacg tcgagatcaa accgccgtac
     1081 ctctaaagaa agtccgatga tgagcgataa tctgtttgtc tcgacggaat ggcttgccgc
     1141 gcatcttggc gaaccagatc tcgtgatcat cgacggtacg ttcttgctgc cgagcgaagg
     1201 ccgcaatgcg cgcgaagaat atcgcgccgg gcatattcct ggcactgtgt tcttcgatat
     1261 cgacgagatt gccgaccaca gcatagatct gccgcatatg ctgccgactc ccgaagcctt
     1321 cgcaaaggcg gccggcgcgc tgggcatcaa cgacatgtcg cggatcgttg tctatgacac
     1381 aagcaatttt cagggcggcg cgcgcgtctg gtggaccttg cgcctttatg gcgcgaagga
     1441 cgtgaagctc cttgcgggcg gtctggcgcg atggaaggcc gaaggccgac cgctcgaaca
     1501 gggcgaggct aggccctcgc cgcaaacttt caacgtcacc ttcgacgcgg cgggcgttgc
     1561 gggattcgaa gacgtacgca agatcatgca ggatcattcg cgcgagatcg tcgatgcccg
     1621 cccggccgcg cgttttcgcg gccaaacgcc ggagccgcgt ccgggcctga gctcgggcca
     1681 tatccccggc agcttcagcg tgccttcgcg cgacctcgtc gaagacggcg agatgaaatc
     1741 gccgtaagag gtcgcagccg cattcaccaa ggccggcgtc gatctctcgc ggccggtgct
     1801 cacgacatgc ggttcgggcg tgacggcggc gatcctgttg cttggtcttg cgaccctcgg
     1861 caagcacgac gtgcgcctct acgacggctc atggagcgaa tggggcgccc ggcccgatgc
     1921 acccgtcgaa aagggctagg tcagccccgc cgcgagcttt gcggttcacc gatttattat
     1981 ggtcggcctg tcgaatttca cggccaggga acgctttgta acgctcccgg ttatcgacag
     2041 catcatagcg caatctcgtg aagtcattcg caaagaactt gacgtacttc gcagtcgcgc
     2101 taccatgcac cagctacggc gagtcatttg aaaccattga tggtttttga tgcttgccca
     2161 tgggatcgcc cgatggtcgt cgggcgttgt ttggttcctt cacgcggcgc aaggccgcaa
     2221 ctctcctgag gaatcgaacc gggttttagt ccgtccccga tctcccccgc atggggagtc
     2281 ctgatcgcac aatccaacga
//
LOCUS       DASWOG010000696         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_255873,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000696 DASWOG010000000
VERSION     DASWOG010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255873"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VGB93_14265"
     CDS             complement(<1..115)
                     /locus_tag="VGB93_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0147281.1"
                     /translation="MQEEIQRMILEGEFEPGEKLGEVELAMRLGVSRGPVRE"
     gene            387..1358
                     /locus_tag="VGB93_14270"
     CDS             387..1358
                     /locus_tag="VGB93_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY0147282.1"
                     /translation="MRKLAILDDYQGVALQFADWSAVGAAADITVFREAFKSEQQIAQ
                     ALSGFEMVCLMRERTPFPASLIEKLPALNFIGVTGPHNRVLDLAAARKHHIVVSCTRG
                     GASEFPTTELTWALILAAARHLPQEDRALRAGAWQTTIGVTLHGRTLGLLGFGRIGQR
                     VASIGNAFGMKVIAWSPNLTPERAAGGGATYVSKDELLSQSDVLSLHMVLSDKSRGIV
                     GADEFKKMRPDAILVNSSRGPLVEEQALLAALKSQRIRAAALDVFDQEPPPLDHPLRI
                     LDNVVLTPHLGYVTEEVYSTFFNDTVKNALAYLAGRPIRLLQPEDQA"
     gene            1393..>2511
                     /locus_tag="VGB93_14275"
     CDS             1393..>2511
                     /locus_tag="VGB93_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0147283.1"
                     /translation="MAAPWINSLQPFSKLAEKFKAGQDTPRHFLERCIAEIEQKDRAL
                     RAFVHLDIDRARRAADDSSVRYRMQRTLSCLDGLPVGIKDIIETRDQPTQMNSPIFAG
                     FQPRRDAACVLALKKAGAIILGKTATAEFACGRSPETRNPSDYTRTPGGSSSGSAAAI
                     GAGLIPAALGTQTQGSTIRPASYCGVYGLKPSHSHLSLDGVAPLSPTLDHLGLLGSSL
                     TDIWNMVTVIAKVAPGPHNSRTSDFLPPQPAAPRRLIRLDTKGWAETDEASKHAFESA
                     VVALKRAGVEVIDRKTETRVDALERDLLAADEAATKIYSYEAQWPLRAYAACGEAAVG
                     TRILQLIEIADQMPRADYERAIAAREKLRATLQTFAGKI"
BASE COUNT          525 a          767 c          695 g          524 t
ORIGIN      
        1 cctcccgtac cggcccgcgg ctcactccta gccgcatcgc caattccact tcgcccaatt
       61 tttcgccggg ctcaaattct ccttcaagaa tcatgcgctg aatttcttcc tgcacgactt
      121 ggggcagcga actgccgcgc agaatttgca gagccgaacc gcctttttcc aaagaattcc
      181 tcccagtgat tcaccgcggt ccggcaattt tcagtcgaac ttccgatctc aatcgagtcc
      241 taaacgtacg cgtaacacta gcatagccat aaatatgttg acaatcttca tgtcaccgct
      301 gagagtgaag gcgggatggt aggactgtca acaatcctta ttgtcgtttc tggccttatg
      361 cgcgaattct cctagggatc tgtggcgtgc gcaaattggc gatcctggat gattatcagg
      421 gcgtagcact gcagttcgcc gactggtcgg cagttggcgc cgccgctgat atcacggtgt
      481 ttcgagaagc gttcaaaagt gagcagcaaa tagcgcaggc gctttcggga ttcgagatgg
      541 tgtgcctgat gcgtgaacgc accccatttc ccgcgtccct gatcgaaaaa ctccctgccc
      601 taaattttat cggcgtcact ggaccgcata atcgcgtctt ggacctcgct gccgctcgga
      661 aacaccacat cgttgtctcc tgcacgcgtg gtggcgccag cgaattcccc acaacggaac
      721 tgacctgggc gctcattctc gccgcagccc ggcatcttcc gcaggaagat cgtgcgctac
      781 gcgccggcgc ctggcaaacg acgatcggcg tcactcttca cggcaggacg ctggggcttc
      841 tcgggtttgg gcgcataggg caacgcgtcg cctctatcgg caacgccttc ggcatgaaag
      901 tcattgcgtg gagcccgaat ctcactcccg aacgcgctgc tggcggcggc gcgacctatg
      961 tcagcaagga cgagctgtta agccaaagcg atgtcttaag cctgcatatg gtgctgagcg
     1021 acaagagcag aggaatcgtc ggcgcggatg agttcaaaaa aatgcgaccg gacgccattc
     1081 tcgtgaattc gtcgcgcggt ccccttgtcg aggaacaggc cttgctcgcg gcgctcaaga
     1141 gccagcggat tcgtgccgcg gcgcttgatg tttttgatca agagcccccc cccttggatc
     1201 atcctctgcg aatcctcgac aacgtcgtgc tcacgcctca cctcggctac gtgactgagg
     1261 aggtctattc gacgttcttt aacgacaccg tgaaaaacgc cttggcctat cttgcgggcc
     1321 ggcccatccg ccttttgcag cctgaagatc aagcatgacc cttccgaagc gcctacgagg
     1381 ttttgtttag ggatggccgc cccctggata aattccctac agcccttttc aaagctggcc
     1441 gagaagttca aagcaggaca ggatacgccg cggcatttcc tagaacggtg cattgcggaa
     1501 atagaacaga aggacagagc tcttcgcgca ttcgttcatc tcgatatcga tcgggcacgc
     1561 cgtgcggcgg acgattcaag tgtccgctac agaatgcagc gcacgctctc ctgcctggat
     1621 gggctgccgg ttgggatcaa ggatatcatc gagacgcgcg atcagccgac gcagatgaac
     1681 agtcccatct ttgccggatt ccagccgcgg cgggatgcgg catgcgttct cgccttgaag
     1741 aaagccggcg ccattatcct cgggaaaact gcgacggcag aatttgcctg cggacgttcg
     1801 ccggaaacac gtaatccttc cgattacacc cgtacgccgg gtggctcctc cagcggttcg
     1861 gcggccgcaa ttggcgccgg cttgataccc gcggcgctgg gaacacaaac ccaaggctcg
     1921 acaatccgac cagcctccta ttgcggcgtt tatggattga agcctagcca cagccatctt
     1981 tcgctcgatg gcgttgcgcc cttgtcaccg accctcgatc atctcggact tttgggatcg
     2041 agcttaactg atatctggaa tatggtgacc gtcatcgcca aagtcgcgcc gggcccacac
     2101 aattctcgaa cgagcgattt tctgcctccg caacctgcgg cgccgcgacg cctgatacgc
     2161 cttgacacca aaggctgggc ggagaccgac gaggcctcga aacacgcatt cgaaagtgcc
     2221 gtcgttgcct tgaagcgcgc cggcgtggaa gtcattgacc gcaaaaccga gacgcgcgtt
     2281 gatgcgctag aacgcgatct cctcgccgcc gacgaagccg cgacaaaaat atattcctac
     2341 gaggcgcaat ggccgcttcg agcctatgcg gcttgcggcg aggcggccgt cgggacgcgt
     2401 attctgcaac tgatcgagat cgccgatcag atgccacggg cggattacga gcgcgcgatt
     2461 gccgcgcgcg aaaaattgcg cgccacgctc caaacgtttg cgggaaagat c
//
LOCUS       DASWOG010000697         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_17913,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000697 DASWOG010000000
VERSION     DASWOG010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17913"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGB93_14280"
     CDS             <1..180
                     /locus_tag="VGB93_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HEY0147284.1"
                     /translation="GVEGRTAHEIFGSPDDLKFRSSMTLFAVVAIGQTIFAAALRKYF
                     VVGDDRTLEILACHF"
     gene            347..517
                     /locus_tag="VGB93_14285"
     CDS             347..517
                     /locus_tag="VGB93_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147285.1"
                     /translation="MGVIAMLVRRGRVFFRFGVPAVFVIVRGFAVMMRGTFVMRRRRV
                     MIFTGGIALSWP"
     gene            complement(630..706)
                     /locus_tag="VGB93_14290"
     tRNA            complement(630..706)
                     /locus_tag="VGB93_14290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Arg,seq:cct)
     gene            882..1502
                     /locus_tag="VGB93_14295"
     CDS             882..1502
                     /locus_tag="VGB93_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147286.1"
                     /translation="MPRRRERAGGRGFGGRASRTRFDRWTGFTRAGVEPVRPTPDNPD
                     FDMAAREALRSAVKDGLDIVTLARPDRWGLIPVFAFLPPMSAAIPEQSLAVWLLARGD
                     GRFMPEREANICRGVFLDAENAARVARHGLWNDPYYAVIAATDQSVFAEEAATNVIAK
                     AGSLISTCGQSASIFSSALAGLVDSQSRSCNATSGYLTGQVWAFML"
BASE COUNT          280 a          478 c          507 g          382 t
ORIGIN      
        1 ggcgtcgaag gccgcacagc tcatgagatt ttcggcagcc ccgatgatct caagttccgc
       61 tcgtcgatga cgttgttcgc ggtcgtcgcc ataggtcaga cgattttcgc cgctgcatta
      121 cggaaatatt tcgtcgtcgg cgatgaccgc acgctcgaaa tacttgcgtg tcacttctga
      181 tcttcacgct gtgcgcattg ccccgcacca ggctccgtga tggggccacg catgacagcg
      241 aaaggtaaac ggggcgagag attggcctct cgccccgcat caattgtctg aatcgttgca
      301 aattggctta cttgtgtgaa tgctcatgcg ccgtggcggt atgcttgtgg gcgtgatcgc
      361 tatgctcgtg cgcagaggtc gcgtgttctt tcgcttcggc gtgccggccg tgttcgtgat
      421 cgtgcgcggc ttcgcggtga tgatgcgcgg cactttcgtg atgcgccgcc gccgcgtgat
      481 gatattcact ggagggattg ctttgtcttg gccataacgt ctcctcgctg tgcttatctg
      541 ccctctcgag ggggcatcaa gcacttggtg aggcgccgca tcggattcaa cgtgcatcta
      601 cgaacgcgtt cgccagcggc ggcgaacgat ggcgtcccgg aagggagtcg aacccctgac
      661 ctacggttta ggaaaccgtt gctctatcct gctgagctac cgggacttgc cgtgagactt
      721 gccaacgaca cctgccatga gctgcgtcac gccttacctt accatgacaa aatgacgtct
      781 tccagccgcg gggcacagta cacggtgaag cgtccgccgt ttcttcgcgc aattgcgctt
      841 gtcgcttcag tagcgatgtc gcccgttaag gcgcagcaat tgtgccccgt cgccgggaac
      901 gagcgggtgg ccgtggcttc ggtggacgag cgtctcgaac tcgctttgac cgatggacgg
      961 gttttacgcg tgccggcgtc gagccggtac gcccgacacc cgacaatccg gatttcgata
     1021 tggccgcgcg ggaagcttta cgctccgcag tcaaagatgg tctcgatatt gttacactcg
     1081 cgcggccgga tcgctggggg ctcatcccgg tctttgcctt cctgccgccg atgtcggccg
     1141 ccattcccga acaatcgctt gcagtctggc tgttggcgcg gggtgacggg cgtttcatgc
     1201 cggagcggga agcaaatatt tgccgcgggg tctttcttga cgcggaaaat gcggcgcgtg
     1261 tggcccggca cggcctctgg aacgatcctt attatgctgt catcgctgcg accgaccaga
     1321 gcgtctttgc cgaagaggcc gcgaccaatg tgattgcgaa ggcagggtca ctgatatcga
     1381 cgtgcggcca aagcgcctct atcttctctt cggccctcgc gggattggtg gattcgcagt
     1441 cacgatcctg caacgcgacg tcaggatatt tgacggggca ggtttgggct ttcatgctct
     1501 gatcggccag aggattcgtg tccgcggtct gctcgatctg cgttttggac cgcagatcga
     1561 agtatccgaa cctgacgcca ttgaaatcgt tccccagctc caggttgcgc ctggcgcggg
     1621 ccaacatgct gaggcaaaag ctgctga
//
LOCUS       DASWOG010000698         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_163728,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000698 DASWOG010000000
VERSION     DASWOG010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163728"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGB93_14300"
     CDS             complement(<1..983)
                     /locus_tag="VGB93_14300"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0147287.1"
                     /translation="MSREVVIVGAARTAVGAFNGAFAAIPAHQLGAAAISAALQRAKL
                     DPSEVDEVILGQVLGAGEGQNPARQAAMKAGVPKEKTAFGLNQVCGSGLRAVALGMQQ
                     IANDDAKIIVAGGQESMSLSPHVAHMRAGTKMGDVKFIDLMIKDGLTDVFNNYHMGTT
                     AENVAAKWQISRDDQDKFALGSQNKAEAAQKAGKFADEIASFTVSTRKGDIVVDSDEY
                     IRAGTTLEALTKLKPAFSKDGTVTAGNASGLNDGAAALVLMSADEARSRGLTPLARIA
                     AWATAGVDPSVMGSGPIPATRKALEKAGWKVKDLDLVEANEAFAAQALAVN"
     gene            1205..1810
                     /gene="phaR"
                     /locus_tag="VGB93_14305"
     CDS             1205..1810
                     /gene="phaR"
                     /locus_tag="VGB93_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY0147288.1"
                     /translation="MANDKQPTTIKKYANRRLYNTGTSAYVTLEDLAEMLKKGEDFVV
                     YDAKTGEEITRSVLTQIIFEQEGKNGQSLLPIAFLRQLIRFYGDSMQMLVPSYLEFTI
                     DKLTTDHHKFREQFETALGVSPLTVPTRQMFGSLEEQTRKNMALFNQALTMFTPFGAA
                     KRTSEAEASEATEGSDKADLDNMKRQLDELQKRLEQLVPKE"
     gene            complement(1842..2612)
                     /gene="gloB"
                     /locus_tag="VGB93_14310"
     CDS             complement(1842..2612)
                     /gene="gloB"
                     /locus_tag="VGB93_14310"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY0147289.1"
                     /translation="MSLEIHQFLCLSDNLCVLLHDPATGATAAIDAPDAPPILAALAT
                     KGWVLSDILLTHHHADHIQGVPGLKAAFPDARISGPAREADRIGHLDQPLNDGDTVMI
                     GMHAAKVLAVPGHTRGHIAYWFADDRLLFAGDTLFAMGCGRPFEEPPSILYRSLMKLA
                     ALPRETQIYCGHEYTLANARFALSVDPGNAALRTRAEDVGGLRAEGRLTLPTRMDIEL
                     ATNPFLRAGNTDIQQSLGLEDVPAEEAFAVLREAKNRA"
     gene            2739..3479
                     /locus_tag="VGB93_14315"
     CDS             2739..3479
                     /locus_tag="VGB93_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0147290.1"
                     /translation="MSLDIVDLRSFYDSPLGKATERFVTQAIRTRFDTCVGQTVLGVG
                     YAAPYLEMFRGDAVRVLAFMPAEQGVVNWPAPGPSASALIETSMMPLPDSCIDRVILI
                     HALEIAEHPRELLGEIWRILTPGGRLLAIAPRRAGLWAQLDTTPFGNGQPFSRSQLRD
                     LMRETLFSPTHWGEALYFAPFEGKIVLRSAPLFEKIGAKLSLPGAGIIFVDAMKQLYR
                     PIGVRRTSHALQPLQPVLGTRPAGIAPG"
     gene            complement(3484..>3640)
                     /locus_tag="VGB93_14320"
     CDS             complement(3484..>3640)
                     /locus_tag="VGB93_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEY0147291.1"
                     /translation="EQLAEADIPVQWHLAPGLGHGIDGSGLLHGGLFLAKSFGLKLEL
                     PPGPIPR"
BASE COUNT          708 a         1145 c         1064 g          723 t
ORIGIN      
        1 ttgttgacgg caagcgcttg cgccgcgaag gcttcattgg cctcgacaag atcaagatcc
       61 ttgaccttcc agccggcttt ctcgagcgcc ttgcgggtcg ccgggatcgg gcccgaaccc
      121 atgacagacg gatcgacgcc cgccgtcgcc caggcggcga tgcgcgccag cggcgtgaga
      181 ccgcgcgagc gcgcctcgtc ggcactcatc agaaccagcg cggcagcacc gtcgttgagg
      241 cccgaggcat tgccggcggt aacggtgccg tccttggaaa aggccggctt cagtttcgta
      301 agtgcctcaa gcgtcgtacc ggcacggata tattcgtcgc tgtcgacgac gatatcgccc
      361 ttgcgcgtcg agacggtgaa cgacgcgatt tcgtcggcga acttgcctgc cttctgcgca
      421 gcctcggcct tgttttgcga gccgagtgcg aatttgtcct gatcgtcgcg gctgatctgc
      481 cacttggcgg cgacattctc ggcggtggta cccatgtgat aattattgaa gacgtcggta
      541 aggccgtcct tgatcatcaa atcgatgaac ttcacatcgc ccatcttggt gccggcccgc
      601 atatgcgcga catgcggcga cagcgacatg ctttcctggc cgccggccac gataatcttg
      661 gcatcgtcat tggcgatctg ctgcatgccg agcgcgactg cgcgtaaacc cgaaccgcaa
      721 acctgattaa gcccgaacgc cgtcttctct ttcggcacgc cggccttcat cgccgcctgg
      781 cgcgccgggt tctgtccttc gcccgcgccg agaacttgac cgaggatgac ttcatcgact
      841 tcggacgggt cgagtttggc gcgctgcagt gcagcactga tggcggcggc gccaagctga
      901 tgcgccggaa tcgcggcaaa agcaccattg aaagcgccga cagcggtccg cgccgctcct
      961 acgatgacga cctcgcggga cattactcac tcctcttggc caaagcttcg ccccgccgat
     1021 gccgggccta tcgtaaacca ttctctgcat ttccggcttg gcggcgtcaa ggaaatccgg
     1081 gatcgtcctt ttggacgata agaatttcag ttttcgcaaa gccgcaaagg gcttatggtt
     1141 tgctgcgcgg cggtttgcgg gatctttgtt tgatactaat ctctctgacg ggtggttatc
     1201 caacatggct aatgacaagc agcccaccac aattaagaaa tacgccaatc ggcgcctcta
     1261 caataccggc accagcgcct atgtgacgct cgaagacctt gctgagatgt tgaagaaggg
     1321 cgaggacttc gtcgtctatg acgcgaagac tggcgaagaa attacccgtt cggttttgac
     1381 gcagatcatt ttcgagcagg aaggcaagaa cggccagagc ctgctgccga tcgccttttt
     1441 gcgccagctc atccgcttct atggcgacag tatgcagatg ctggtgccga gctatctcga
     1501 attcacaatc gacaagctca cgacggatca ccacaagttc cgcgaacaat tcgagaccgc
     1561 gcttggcgtt tcgccgctca ccgtgcccac ccgtcaaatg ttcggttcgc tcgaagagca
     1621 gacgcgcaag aacatggcgc tgttcaatca ggcgctgacc atgttcacgc ctttcggcgc
     1681 cgcaaagcgc acctccgaag cggaagcttc tgaagcaacc gaaggcagcg acaaggctga
     1741 tctcgacaat atgaagcgtc agctcgacga gttgcagaag cggctcgagc agctcgtccc
     1801 gaaagaataa acgaccgagc tacggaaccg tttgtgcggt ttcaggctcg gttcttggct
     1861 tcgcggagaa ccgcgaaagc ctcttccgca ggaacgtctt caagtcccag gctttgctga
     1921 atgtctgtat taccggcgcg caagaacgga tttgtcgcga gttcgatatc catccgcgtc
     1981 ggcaatgtga gccgcccttc ggcgcgcagg ccgccgacat cttctgcacg cgtgcgcaac
     2041 gctgcgttcc ccggatcgac cgaaagcgca aaacgggcat tggccagcgt atattcgtgc
     2101 ccgcaataga tctgcgtctc gcgcggcaag gccgctagct tcatcagcga tcgatagaga
     2161 attgagggcg gttcctcgaa cggccggccg cagcccatcg cgaataaagt gtcgcccgca
     2221 aagagcaaac gatcgtcggc aaaccaatag gcgatatggc cgcgcgtatg acctggcacg
     2281 gccagaacct tggcggcgtg catgccgatc atcactgtgt cgccgtcgtt caaaggctgg
     2341 tcgagatggc cgatcctgtc ggcctcgcgc gccggaccgc tgatccgtgc atctggaaaa
     2401 gccgccttta agccgggcac accctgaatg tgatcggcgt gatgatgcgt caacaaaatg
     2461 tcggacagaa cccagccttt tgtcgcgagc gccgcgagta tgggtggcgc gtccggcgca
     2521 tcgatggctg cggtcgcccc cgtcgccgga tcatgcagaa gcacgcagag attgtcgctg
     2581 aggcagagga actgatggat ttcgagactc atttgcgccg cctgcaaaca ccgacacgga
     2641 ttaaccaggt ccgggccaat cagcggcgag caacctctgg taacttatag cgcgaaaaag
     2701 gctttccgcg agccggcccg gcatgcaagg aagacagcat gtcgctcgat atcgtcgatc
     2761 ttcgcagttt ttacgattcg cctctgggca aggcgaccga gcgttttgtg acccaggcga
     2821 tccggactcg cttcgacact tgcgtcggcc agacggtcct cggcgtcggc tatgccgcac
     2881 cctatctgga aatgtttcgc ggcgacgctg tgcgggtgct ggccttcatg cccgcggagc
     2941 aaggcgtggt gaactggcct gcgccagggc cctccgcctc ggccctgatc gagaccagca
     3001 tgatgccgct gcccgattcc tgcatcgatc gggtaatcct catccacgcc ctcgaaatcg
     3061 ccgaacatcc gcgcgaacta ctgggcgaaa tctggcggat tttgacgccg ggcggtcggc
     3121 tgctcgccat cgcgccgcgc cgcgccggac tttgggcgca gctcgacaca acccctttcg
     3181 gcaacggcca gcctttttcg cgcagccagc tccgcgacct gatgcgtgag acgctgtttt
     3241 cgccgaccca ttggggcgag gcgctctatt tcgccccgtt cgagggcaag atcgtcctgc
     3301 gctcggcgcc cctcttcgag aagattggcg ccaagctctc actgcccggc gctggcatca
     3361 ttttcgtcga tgcgatgaaa cagctttacc gtccgatcgg cgtccggcgc acgtctcacg
     3421 ctctacagcc tttgcagccg gtgcttggca caagacccgc cggcattgcg ccggggtgag
     3481 cgcctagcgc ggtattgggc ccggcggaag ctccagttta agcccgaacg atttcgccag
     3541 aaacaatccg ccatgcagca ggccgctgcc atcaatgccg tggcccagac ccggcgccag
     3601 atgccattgc accgggatat ccgcctcagc cagttgctcc
//
LOCUS       DASWOG010000699         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_365000,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000699 DASWOG010000000
VERSION     DASWOG010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365000"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VGB93_14325"
     CDS             complement(<1..1631)
                     /locus_tag="VGB93_14325"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0147292.1"
                     /translation="MDHSNPATPQTREFSESALLQSAIEFQSGKQYGIARQLYLQILE
                     QNPDHELAWHNLGLVEHMTGRHAQAAEYIGKAVALKPDYARAYANLAAVLRETRQLEA
                     ARETAERAIRLEPGFAPAYNNLGGILEDLGEMEAALAAYLEACQLDPFFIEAHTSAAE
                     ILRKLGRREEGLRLCAAVSAKRPDAAGPHFAAGNILRDLLRLDEACAEFDQAISLDPD
                     FVGAYCNLGNLLQHRNDFVGAVANYEKALAIKPDFAEIHCNLGMAYESLRRIDEALNC
                     YDRAVALDPSFVGVRLQRLHMRRAICDWDGDDAEEPAALAAAAASDGLVPPFGTLAMN
                     SGHALQLDLARRWARAFRAKPSFDHRRRAPDRNRKLRIGYLSGDFFRHATAILMAGLF
                     EHHDRERFEIIAYSHGGDDGSELRHRLLKAFDRFVDITALDDRAAAARIYADDVDILV
                     DLKGYTQFSRSEIVAHRPAPIQVNFIGYPGTMGADFIDYVIADPIVLPMDQQSYYDEK
                     IVHLPDTYQPNDRQRANDARKPTRAEAGLPVEGFV"
BASE COUNT          345 a          494 c          547 g          337 t
ORIGIN      
        1 aaaacgaaac cttcaacggg caggccagct tcggcgcggg tgggtttacg cgcatcgttc
       61 gcacgttgtc tatcgttcgg ctggtaggtg tccggcaggt ggacgatttt ctcgtcgtaa
      121 taagactgct gatccatcgg caaaacgatt ggatcggcaa tcacgtagtc gatgaagtcg
      181 gcacccatcg tgcctggata accgatgaaa ttcacctgga tcggcgccgg ccgatgcgcg
      241 acgatctcgc tgcgcgaaaa ttgcgtatag cctttaagat caacgagaat atcgacgtcg
      301 tcagcataga tgcgtgccgc cgccgcgcgg tcgtcgagtg cggtaatatc gacgaaacgg
      361 tcgaaagcct tgagcagccg atggcgcagc tcgctgccgt catcgccgcc gtgcgaatag
      421 gcaatgatct cgaagcgctc acggtcatga tgctcgaaca gtccggccat cagaatcgcc
      481 gtcgcgtggc ggaagaagtc gccggagaga tagccaatcc gcaattttcg attgcgatcg
      541 ggcgcccggc ggcggtgatc gaaacttggc ttggcgcgaa aagcgcgcgc ccagcggcgc
      601 gccaggtcca attgcagcgc atgaccggag ttcatggcga gcgtgccgaa gggcggaacg
      661 aggccgtcac ttgctgccgc cgccgccagt gccgcgggtt cttcggcgtc gtcgccgtcc
      721 cagtcacaaa tggcacgacg catgtgcaga cgctgcaagc gaaccccgac aaaagacggg
      781 tcaagcgcga cggcgcggtc ataacaattc agcgcctcat cgatgcgtcg cagcgattca
      841 taagccatcc ccagattgca atgaatctct gcaaaatccg gcttgatcgc gagcgccttt
      901 tcataattgg ccacagcgcc gacaaaatca ttgcgatgct gcaacagatt gccgaggttg
      961 caataggctc cgacgaaatc gggatcgagc gaaatggcct gatcgaactc cgcacacgct
     1021 tcatccaggc gaagcaggtc gcgcaggatg ttgccggccg cgaaatgcgg acctgccgcg
     1081 tccggccgtt tcgccgatac cgcagcgcag agtctcagcc cctcttcgcg acggccgagc
     1141 ttacggagga tttcggcggc gctggtatgc gcttcgatga aaaacggatc gagctggcaa
     1201 gcttccaaat aggcggcgag cgccgcctcc atctcaccca ggtcttccag aatgccgccg
     1261 agattgttat aggccggggc gaagccgggc tccagccgta tggcgcgctc cgccgtttcg
     1321 cgcgccgctt cgagttgccg cgtttcgcgt aaaaccgcgg cgaggttggc gtaggcgcgg
     1381 gcataatccg gtttgagggc aacggctttg ccgatgtatt ccgccgcttg cgcgtgccgt
     1441 ccggtcatgt gctcgacgag gccgagattg tgccaggcaa gttcgtgatc gggattctgc
     1501 tctaggattt gcagataaag ctgacgcgca atgccatatt gcttgccgct ctggaattcg
     1561 atcgcgcttt ggaggagcgc ggattcggag aattcccggg tttgcggcgt ggcgggattg
     1621 gaatggtcca tcggcggcct tggtgcgtcc ggccgccgcc gggcgctgtt cttgagcgta
     1681 gcagaggggg atgcgaaaaa gtggatatcg gtttttcgcg taa
//
LOCUS       DASWOG010000700         8187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_53669,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000700 DASWOG010000000
VERSION     DASWOG010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53669"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1797)
                     /locus_tag="VGB93_14330"
     CDS             complement(82..1797)
                     /locus_tag="VGB93_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0147293.1"
                     /translation="MSLPKGPAHLATDVLVIGSGAAGMAAALAAVREGARVLLADRSL
                     IGRSGATIMAQMTVAVALGEETPDDWEAHFADTMKAGRGLCDANLARLVCERGPAAIR
                     QLDAWNVGWARGPDGHLRQTQAPGHDRPRCVYVDFLSTGPAVAKTLRAQVLKNPQIVR
                     VGDLCITDLVQTDGAVGGAVGFSMADGRAVSIAAAAIVLATGGVTRIYRRNSASANMG
                     GDGHALALRAGAELMDMEFVQFFPIGHLAPRLVGMDPIMWDPFRYKLGGRLLNGEMKE
                     FMSHYDNVQENDRYVVTRDKATYGILKEVEAGRGTPAGGAYLSFQHIPEEKLREAFGP
                     VIDRLAENGIDLTKMPVEVAPIAHYHMGGVRVDEALETRVRNLFAAGELVAGANGANR
                     LSGNAITEALVFGEIAGQGAAQRALSEARTVWHPAAASAALELVEAVSGETDFNATHA
                     IADLQDVMADLVGPFRTRQGLEVALEKVAKLKDASGDQPPGRSEPYNSARLDWFDLRS
                     MLSVAEAIIRAALSREESRGGHQREDFPGMDERWAFHQTVALGHDDLILGRAPVHAEA
                     LAETV"
     gene            complement(1851..2390)
                     /locus_tag="VGB93_14335"
     CDS             complement(1851..2390)
                     /locus_tag="VGB93_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY0147294.1"
                     /translation="MTDDRNEMLRKKLNQPIVSSAHLAAGASPALSEFEFGLILAVHA
                     FERWIVGCMAASGVPNLSKIEVLILHIVRHRDRPKRFSDIARILDIEETHVVAYAVRK
                     LEAAKLVATKRDGKEKMVSVTRQGIEVCTRYGAVREQLLVKPLRSSGPSEDVLSDLGE
                     KLRALSGYYDQAARSAATV"
     gene            2517..6143
                     /locus_tag="VGB93_14340"
     CDS             2517..6143
                     /locus_tag="VGB93_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0147295.1"
                     /translation="MRAGWQLWVERGGTVTDIVARGPDGTLVTHKLLSEDPARYSDAA
                     VAGIKQLLGIALGESIPDGLIETVKIGTTVAPNALLERKGEPTLLLVNRGFKDTLAIG
                     DQTRPRLFDLNVVRPSVLYKRVIEIAGRVDRDGVEIEPLDEATARDALKAARAEGFSA
                     CAIVFLHAWKYPDHERRIAALAHAAGFTQVSASHEVSPLLRLIPRGDTTVVDAYLSPV
                     LRRYVDGVAAELGKVKLYFIQSNGGLIDARRFQGKDAVLSGPAGGIVGAARTAATAGL
                     RNIIGFDMGGTSTDVALYAGAFQRTFDTQVAGVRIRAPMMAIHTVAAGGGSILHFDGA
                     RFRVGPDSAGANPGPASYRRGGPLTITDANICLGKIQPNYFPAIFGPDGDEPLDANIV
                     RIDFDELAERVSASTGIRRDPRDVAEGFVRIAVANMANAIKHISVEKGHDVSRFALHC
                     FGGAGGQHACLVADELGIDKVFIHPFAGVLSAYGIGLADQTVMREQSVELALTPASLA
                     TLADHADALEQAAIRELAEQDVDVSKVLVARKLHVKYQGTDAALLVPLTDYADVIASF
                     SDAHLRQFGFTTLDRPLIAETILVEATYPGETAIAVHPDGRADGAPLEPIGYVEIWSG
                     GRLHDAPVYERADLHAGDELKGPVLIRESAATTVVEPDWALLITEHNNIELRRYKTRA
                     KAADTDAKAAQADPVLLELFNNIFMNIAEHAGSVLQNTSRSVNIKERLDFSCAIFDAE
                     GNLVANAPHVPVHLGAMGESVRTVLRRRGATLRPGDVIALNNPFNGGTHLPDITVITP
                     VFDSEGKSILFFVGSRGHHADIGGLTPGSTPPESHTLEEEGVIIDDFLLLDRGEFRET
                     EFRALLTGARYPARNPDMNVADIKAQVAANETSVQELNRIVFEWGWDVVAAYMRHVMD
                     NAEEQVRKALARLRDCEFSDRLDNGARLRVKISIDRHERSAVIDFTGTGPQQGDNFNA
                     PPAVTRAVVLYVFRCLVGEDIPLNEGCLKPLHIVIPPDTFLSPARGSAVVAGNTEVSQ
                     AVCNVLFGALGVLASSQGTMNNFLFGNETYQYYETICGGGGAGMTPGGEGFDGASAVQ
                     VHMTNTRMTDPEILELRYPVRLIDFSIRRGSGGKGRWHGGDGAVRKIAFLEPMIASLV
                     TSRRKVAPFGLSGGADGLTGRQWVERADGSRTELASTARVDVDAGDVIAIETPGGGGF
                     GADE"
     gene            6243..7505
                     /locus_tag="VGB93_14345"
     CDS             6243..7505
                     /locus_tag="VGB93_14345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0147296.1"
                     /translation="MQKTISATALNASSPDSLTRQHWFVLAITMVLWMFDGYETYALL
                     LTIGPSLHELLPRDELHNLSHFAAYLISLTLFGWAVGGVLGGLIGDRIGRRATMIGAV
                     VLYSIFTGTSALAHSWQILALTRFLTGIGLGAEWGVGASLLQEVWPEKLRTRAAGVLQ
                     AAFSCGFVIASVLWLIIGGAFGGSWRWLYIVGIAPAIFVVITGRAIPESEPWTRATSN
                     ASSAVTLRAYLHTLIIAVIISSSITVAFWAISSWVPTYVAVLVHNPREAVSYAGWAGL
                     LFVIGEIFGCVSFGWLAESWGRRWTLVFFLAGSILITPVVFLLIKGAPLFVLLQIPNG
                     FFTGGIYGWYAVHPPELFPTRIRSSAISIIFNSARFLAMLGPILSSSLIAFFGGYGIA
                     ATIASGIFLIGIVAIFFLPETKGRPLPA"
     gene            7554..>8187
                     /locus_tag="VGB93_14350"
     CDS             7554..>8187
                     /locus_tag="VGB93_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0147297.1"
                     /translation="MKTIRIAVPDLISNSYFPALAAIELGCFRDGGLDGRLDHIFPSP
                     KAFAALRDGTVDLVAAPAHALLSAFPEWHGVKLLAALAQGTYWLLVLRADLKATPGDV
                     NAIKGRRIGAAPMVDLALRQLCIDSGLDLVRDKVDIVPVPRANDPGVSFGIAAAQALD
                     EGSIDGFWANGLGAEYAVRSGKGDIILDVRRGLGPSVAFHYTFPAVAASDR"
BASE COUNT         1545 a         2560 c         2395 g         1687 t
ORIGIN      
        1 gcgtataggg gacctcgtag gtgctgactg acacctctgt cccggctccg ccacgctgga
       61 caataagttt cgcagtttcc atcatacggt ctccgcaagg gcttcggcat ggaccggtgc
      121 ccggccgaga ataagatcgt cgtgccccag cgcgaccgtc tgatgaaaag cccagcgttc
      181 atccatgcct ggaaaatctt cacgctggtg accgccacga ctttcttcgc gcgacagtgc
      241 cgcacggatg atcgcctcag cgacactcag catcgagcgc aggtcgaacc aatcgagccg
      301 tgccgaattg tagggctccg aacgaccggg cggctgatca cctgatgcgt ctttcagctt
      361 cgcgaccttt tcgagcgcga cctcgagacc ctgtcgcgtg cggaacggcc cgacaagatc
      421 ggccatgacg tcctgcaaat cggcaatggc gtgcgttgcg ttgaaatctg tttcgccgga
      481 tacggcttct acaagttcaa gcgctgcgct ggccgccgcg ggatgccaga cggttctggc
      541 ttccgacaat gcccgctgcg cggcgccctg cccggcgatt tcgccaaaca ccagcgcttc
      601 ggtgatcgca ttgcccgaca gacgattggc gccgttcgcg ccggcgacca gttcgcctgc
      661 agcaaacaag ttgcggacgc gtgtctccaa cgcttcgtcc acgcgcacac cgcccatgtg
      721 ataatgcgcg atcggcgcga cctcgaccgg cattttggtg agatcgatac cgttctcagc
      781 caaccgatcg atgaccgggc caaaggcctc gcgcaatttc tcttccggga tatgctgaaa
      841 gctgagatag gcgcccccgg ccggcgtgcc ccgtcctgcc tccacttcct tgagaatacc
      901 gtaggtcgcc ttatcgcgcg tcacgacgta gcggtcgttt tcctgcacat tgtcgtaatg
      961 gctcatgaat tccttcatct cgccattcag caaccggccg ccgagtttgt aacgaaacgg
     1021 atcccacatg atcgggtcca tcccgacgag ccgcggcgcg agatggccga tcggaaaaaa
     1081 ctgcacgaac tccatatcca tcagctctgc tcctgcgcgc agcgcgagag catggccgtc
     1141 gccacccata ttggcggagg cgctgttgcg gcggtaaatc cgcgtcacgc cgccggtcgc
     1201 cagaacgatg gccgcggcgg caatcgaaac agcgcggcca tcggccatcg aaaagccaac
     1261 cgcgccgcca acagcgccat cggtctgaac aagatccgta atgcagagat cgcccacgcg
     1321 gacgatctgc ggatttttga gaacctgcgc gcgcaacgtc ttcgcgaccg cgggccccgt
     1381 cgaaaggaaa tccacataca cacagcgcgg ccgatcatga ccgggcgcct gcgtctgacg
     1441 cagatgcccg tccggcccgc gtgcccagcc gacattccac gcatcgagct gacgaatggc
     1501 cgccggcccg cgctcgcaaa ccagccgcgc caaattcgca tcgcacagac cgcggccggc
     1561 cttcatcgta tccgcgaaat gcgcttccca atcatcaggt gtttcttcgc caagcgccac
     1621 ggcgacagtc atctgcgcca tgattgtcgc accgctgcgg ccgatgaggc tgcggtcagc
     1681 cagcaacacg cgtgcacctt cgcgcaccgc ggcaagcgcc gccgccatgc cggccgcgcc
     1741 cgagccgatc acaagaacat cggtggcgag gtgggcgggc cccttcggca aactcatctc
     1801 caaaactcct tgaacttcgg cggtcgcacc cgcgacacgc cgcgagacga tcagaccgtc
     1861 gccgccgatc gcgccgcctg atcgtagtag ccggagagcg cgcgaagctt ctcgcccaaa
     1921 tccgagagca cgtcttccga cggaccggac gaacgcagcg gcttcaccag caattgctcg
     1981 cgaaccgccc cgtaacgcgt acaaacctcg atgccctgac gcgtcaccga gaccatcttt
     2041 tccttgccgt cgcgcttcgt cgcgacaagc ttcgcggcct caagcttgcg caccgcatag
     2101 gcgacgacat gcgtttcttc gatatccaga atgcgggcaa tatccgagaa acgcttgggc
     2161 cggtcgcgat gccgaacgat atgtaagatc aaaacttcga tcttcgagag atttggaacg
     2221 ccggatgctg ccatgcagcc cacgatccaa cgctcgaagg cgtggacggc cagaatcaag
     2281 ccgaattcga actccgacag cgcgggcgat gcacccgcgg ccagatgggc ggaggatacg
     2341 atcggctgat tgagcttttt gcgcagcatt tcattgcggt catcggtcat gcaacggttc
     2401 cgtttccaac aaattcatat tttgtcgata atttttcaag aagaaatata ttctatgttg
     2461 acaaaacatc tgtaactcat tcttgatgca agatcgtttt gaggaataag gtggcgatgc
     2521 gggccggctg gcaattatgg gtcgagcgcg gcggcacagt tacggacatc gttgcccgcg
     2581 ggcctgacgg aacgctcgtc acccataagc ttctcagcga agatcctgcg cgctattccg
     2641 acgcggccgt cgccggcatc aaacaattgc tcggcattgc tctcggcgag agcattcccg
     2701 acggactcat cgagaccgtc aagatcggca cgacggtcgc gcccaacgcg ctgctggagc
     2761 gcaagggcga gccgacgctg ctcctcgtta atcgtggttt caaggatacc ctggcgatcg
     2821 gcgatcagac gcggccgcgt ttgttcgacc tcaatgttgt tcggccgtcg gttctttata
     2881 agagggtgat cgagatcgcc ggccgcgtgg atcgcgatgg cgtcgaaatc gaaccgctcg
     2941 acgaggccac cgcgcgagac gctttgaaag cggcacgcgc agaaggcttt tcagcctgcg
     3001 cgattgtttt cctgcatgcg tggaaatatc ccgatcacga acgccgcatc gcagcgctgg
     3061 cgcatgctgc tggatttacc caagtctcgg cgagccacga agtcagcccg ttgcttcgac
     3121 tcatcccgcg tggcgatacg accgtcgtcg atgcttatct ctcgcctgtc cttcgccgct
     3181 atgtcgatgg tgtggccgcc gaacttggca aggtaaagct ctacttcatt caatcgaatg
     3241 gcggcctgat agacgcccga cgttttcagg gtaaggatgc cgtgctgtcc gggccggccg
     3301 gtggcattgt cggcgctgcg cgcacggcgg cgacagccgg cctcagaaat atcattggct
     3361 tcgatatggg aggcacatcg accgacgtgg ccctctatgc cggcgcgttt caacgcacct
     3421 tcgatacgca agtcgctggc gtacgcatcc gcgcgccgat gatggcgatc catacggtcg
     3481 cagcgggcgg cggctccatc ctccatttcg atggcgcgcg tttccgtgtc ggaccggatt
     3541 cggccggcgc caatccgggg ccggcgagct accggcgcgg cggtcctttg accatcacgg
     3601 acgcgaatat ctgcctgggc aagatccagc caaactactt ccccgcgatc ttcggcccag
     3661 atggagacga gccgctcgat gcgaacatcg tgcgcatcga cttcgacgaa cttgccgaac
     3721 gcgtcagcgc ctcaacgggt atccggcgcg atccgcgcga tgtggctgaa ggcttcgtcc
     3781 gcattgccgt cgccaatatg gccaacgcca tcaaacatat ttctgttgaa aaaggccacg
     3841 acgtcagccg ctttgcgttg cattgcttcg gcggtgcggg cggccagcac gcttgcctcg
     3901 ttgcggacga gcttggcatc gataaagtct tcatccaccc cttcgccggc gttctgtcgg
     3961 cctacggtat cggcctcgct gatcagaccg tgatgcggga gcaatcggtc gaactggcct
     4021 tgacgccagc cagccttgcg acgctcgcgg accatgccga cgcgttggaa caggccgcga
     4081 tccgggaact cgcagagcag gacgtcgatg tcagcaaggt ccttgtagcc cgcaagctgc
     4141 acgtgaagta tcagggcacg gacgctgcgt tgcttgtgcc gctcaccgac tatgccgatg
     4201 tgattgcatc tttcagtgat gcgcatcttc ggcagttcgg tttcacgaca ctggatcgcc
     4261 cgctgattgc ggaaacaatt ctggtcgagg ccacctaccc tggcgagacc gcaatcgccg
     4321 ttcatcctga cggccgcgct gacggcgcgc cgcttgaacc catcggctat gtggaaatct
     4381 ggagcggcgg ccgcctacat gatgcgcctg tgtacgaacg ggcggacctg cacgccggcg
     4441 acgaactcaa aggcccagtg ctgattcgcg aatccgcggc gacaaccgtc gtcgaaccgg
     4501 attgggcttt gctgatcacg gaacacaaca atatcgagtt gcggcgctac aagacgcgcg
     4561 cgaaagccgc cgatacggat gcaaaggccg cccaggccga tcccgtgctg ctcgaattgt
     4621 tcaacaacat cttcatgaat atcgcggagc acgccgggag cgttctccag aacacgtcgc
     4681 gaagcgtcaa catcaaagag cgactagatt tctcctgcgc gatcttcgat gcagagggaa
     4741 atctcgtcgc caacgcgccg catgttcctg tccaccttgg agcgatgggc gaaagcgtgc
     4801 gcacggtgct gcggcggcgc ggggcgacat tacgccccgg cgatgtgatt gcgctcaaca
     4861 atcccttcaa cggcgggacg catctgcccg acatcaccgt gatcacgccc gtcttcgaca
     4921 gcgagggaaa atcaattctc ttttttgttg gcagccgcgg acatcacgcc gatatcggcg
     4981 gtctcacgcc gggctcgaca ccgcccgagt cgcatacgct tgaagaagaa ggcgtcatca
     5041 tcgacgactt cctcctgctc gatcgcggtg aatttcgcga aaccgaattt cgcgcgttgc
     5101 tgacgggcgc gcgatatccg gcacgcaatc ccgacatgaa tgttgcggat atcaaggcgc
     5161 aggtcgcggc gaatgaaaca agcgtgcagg agctgaaccg gatcgtcttc gaatggggat
     5221 gggacgtcgt cgcggcctat atgcgtcacg tcatggacaa cgcggaggag caggttcgca
     5281 aggcgctcgc gcggctgcgc gattgcgaat tcagcgacag actggataac ggcgcacgct
     5341 tgcgcgtgaa aatctcgatt gaccgacacg agcgatcggc ggtgattgat tttaccggaa
     5401 cggggccgca gcaaggcgat aatttcaacg cgccgcctgc cgtgacgcgc gccgtcgtgc
     5461 tctacgtatt ccgctgtctc gtgggtgagg acattccgct caacgaaggt tgtctgaagc
     5521 ctctgcacat cgtcattccg ccggacacat tcctatcccc ggcgcgtggc tccgcggtgg
     5581 tcgccggcaa cacggaggtg agccaggcag tttgcaacgt gttgtttggt gctctcggcg
     5641 ttctcgcgtc gagccaaggc acgatgaaca actttctctt cggcaacgaa acataccaat
     5701 attacgagac gatctgcggc ggtggtggcg ccggaatgac gccgggcggc gaaggctttg
     5761 acggcgcctc agccgttcag gtccatatga ccaacacgcg gatgaccgat cccgaaattc
     5821 tcgaattgcg ctatcccgtg cgcctcattg atttttcgat ccggcgcggt tccggcggca
     5881 aaggcagatg gcatggcggc gacggcgccg tgcgaaaaat cgctttcctc gaaccgatga
     5941 ttgcttcgct cgtcacctca cgccgcaagg tcgcgccgtt tggcctcagc ggcggtgccg
     6001 acggtctcac cgggcgccaa tgggtggaac gcgccgacgg cagccgcacg gagcttgcat
     6061 cgacggcgcg tgtggacgtt gacgcgggcg acgtcatcgc aatcgaaaca cccggcggtg
     6121 gcggtttcgg cgccgacgaa taattatgtc tgacttttga aggcaccttg tgaatattcc
     6181 gcacacatat tatagactaa atcagatgat cggacattaa gagcgtaaga cacgctgaca
     6241 acatgcagaa gacaataagc gccacggcct tgaacgcatc cagtcccgac agcttgacgc
     6301 gccagcattg gtttgttctt gcgatcacga tggtgctctg gatgttcgat ggctacgaga
     6361 cctatgcgct gcttttgacg ataggaccgt cgctgcacga attgcttccg cgtgacgaac
     6421 tccacaatct ttcgcatttt gccgcttatc tgatttcgct cacgctcttc ggatgggcgg
     6481 tcggcggcgt tctcggtgga ctgattggcg atcgcattgg ccgccgcgca acgatgatcg
     6541 gcgccgtcgt tctctacagc attttcaccg gaacaagcgc tctcgcgcat tcctggcaaa
     6601 tccttgcgct gacccggttt ctcactggca tcggccttgg tgcggaatgg ggcgtcggcg
     6661 catcattgct gcaggaagtc tggccagaaa aactgcggac acgcgccgcg ggcgttttgc
     6721 aggccgcgtt ttcgtgcggc ttcgttatcg cgtcggttct ctggctgatc atcggcggcg
     6781 cttttggcgg ttcgtggcgc tggctctata tcgtcggcat tgccccggcg attttcgtcg
     6841 tcatcaccgg acgcgcgatt cccgaatccg agccctggac gcgtgcaacg agcaacgcta
     6901 gttccgcagt tacgcttcgc gcctatctcc ataccttgat tatcgccgtg atcatctcaa
     6961 gttcgatcac tgtcgccttc tgggcgatct cttcctgggt gccgacttat gtcgcggtgc
     7021 tcgtgcataa tccgcgtgag gccgtgagct acgcgggctg ggccggactg ctctttgtga
     7081 tcggcgagat ttttggctgt gtcagttttg gatggttggc tgaatcttgg ggccggcgct
     7141 ggactctggt tttcttcctt gccgggtcaa tcctcatcac gcccgtcgtg tttctgctca
     7201 taaagggcgc accgcttttt gtcctgctgc agatcccgaa cggttttttc accggcggca
     7261 tttacggatg gtacgctgtt catccgcccg agcttttccc gacccgcatt cgctccagcg
     7321 cgatcagcat tatcttcaat tcagcgcgct ttctcgcgat gctggggccg attctgtcgt
     7381 cgagcctgat cgcttttttc ggcggctatg gcattgccgc gaccatcgcc tcaggtatct
     7441 tcctcatcgg gatcgtcgcc atattcttcc tgccggaaac aaaggggcgt cccctccccg
     7501 cgtaaacggt tgccgacaat tttgctctcc tcctcaattc gcggcgcact ttgatgaaga
     7561 cgatacgtat cgctgtgccg gacctgatct cgaactccta ctttccggcc cttgccgcga
     7621 tcgagctcgg ttgctttcgc gatggaggtc tcgacggccg gctcgaccat atatttccga
     7681 gcccgaaagc tttcgctgcg cttcgagatg gaacggtcga tctcgtcgcc gcgcctgcgc
     7741 atgccctgct ttcggcgttt ccggaatggc atggcgtcaa gttgctcgcc gcgctcgcgc
     7801 aaggaacata ttggctcctc gtgctacgcg ccgatctcaa ggcgacgccc ggagacgtga
     7861 atgcgatcaa gggtcgccgg atcggggccg cgccgatggt cgatctcgcg cttcgtcagc
     7921 tttgcatcga ttcaggcctc gatctcgtgc gcgataaagt ggatatcgtg ccggtgccac
     7981 gcgcgaacga tccaggggtc tcatttggca tcgcggcggc gcaagcgctg gatgaaggat
     8041 caatcgacgg attttgggct aatggccttg gcgccgaata tgcggtgcga agcggcaagg
     8101 gcgatatcat tctcgatgtc cggcgcggcc tcggtccatc agtcgctttt cactatacct
     8161 tccccgcagt cgcggcgagc gaccgcc
//
LOCUS       DASWOG010000701         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_53681,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000701 DASWOG010000000
VERSION     DASWOG010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53681"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..717)
                     /locus_tag="VGB93_14355"
     CDS             complement(136..717)
                     /locus_tag="VGB93_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0147298.1"
                     /translation="MNDAVKILRREVVSRGFGLLERVTFWRRKYDGKPQEIEREFYDT
                     GPGATILLYDPERRCILLVRQFRPAVYLVEGRDSLLETCAGKLEGAEAPERIVMEVLE
                     ETGVKIGAPTFLFSAYMSPGVYAEKISFFTARYSARDRIAAGGGLANEGEDIDVAEPS
                     LEEALEMIARGEIEDAKTILLIHYAVLHKLMES"
     gene            complement(701..1510)
                     /locus_tag="VGB93_14360"
     CDS             complement(701..1510)
                     /locus_tag="VGB93_14360"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY0147299.1"
                     /translation="MMTDTNANAPGRRIAFLSANTPEAEAARLRLIELYGDSDPETAD
                     VVVALGGDGLMLQTLHRFMHTGKPIYGMNRGSVGFLMNDYREDDLIQRIDDAESSVIH
                     PLAMIATDIHGKIATAMAINEVSVLRQSYQASKIRISVDGKERLLELIADGVLLATPA
                     GSSAYNLSVDGPILPLNSPLMALTPISAFRPRRWRGALLPDSAHVTLDVLEAVKRPVA
                     AVADHFEFKEVIKVEIAMDHATNLVILHDPGHSLDERILREQFGFDDERRG"
     gene            complement(1571..1957)
                     /locus_tag="VGB93_14365"
     CDS             complement(1571..1957)
                     /locus_tag="VGB93_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HEY0147300.1"
                     /translation="MPIFVEIAAGELIDKITILEIKLDQINETAKRVNIRREYEILSG
                     VLQEQITPGEQLSELTAALKAANQELWHIEDDIRDHERAKDFGPEFIALARSVYQTND
                     RRAVLKREINALLQSAIIEEKSYAAY"
     gene            2083..>3717
                     /gene="glyS"
                     /locus_tag="VGB93_14370"
     CDS             2083..>3717
                     /gene="glyS"
                     /locus_tag="VGB93_14370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY0147301.1"
                     /translation="MPDFLLELFSEEIPARMQVQAAEDLKKLVTEVLAAQNIFGESAG
                     SFVTPRRLALHIEGLPPRQRDTKEVRKGPRTDAPAAAINGFLKSAGLTNIAEAQIESD
                     PKKGDFYVATIERPGRATGEVLADILPPILSNFPWPKSMRWGAQSAQPEAFRWVRPLH
                     SIVATFGPETEEPEIVAFSIAGITAGNVTHGHRFMAPGAIRVRRFDDYVASLGKAKVV
                     LDAARRRDIILNDARNLAHAQGLELVEDAGLLDEVAGLVEWPVVLIGAFDAEFLALPP
                     EVIRATIRANQKCFVLRDAATGALANKFVLVANIEAPDGGTAIAAGNARVVRARLSDA
                     KFFYDTDLQVKLEDRLPKLENIVFHAKLGSQAERVKRLAELARWLAPHVGADAEKAAH
                     AARLAKADLVTEMVGEFPELQGLMGQYYARAHGEAADVAAAIGEHYRPQGPSDSIPTA
                     PVSIAVALADKFDTLVGFFAIDEKPTGSKDPYALRRAALGIIRIILENNLRLHITAAI
                     EPAREGYSNLKSVPNYFASGVLGFFADRLKVYLRDKG"
BASE COUNT          691 a         1130 c         1137 g          759 t
ORIGIN      
        1 cgagcgtatt gcgctgtccg acttgtctag tgcggacgaa ccgccgctac tgctcgttga
       61 tcaggacgcg gccgaaacga tgccagtcgg catcaccgtc gatctcgctg ccatcgaggc
      121 tgaactcgcg gcggcttagc tctccatcaa tttatgcagc acggcgtaat ggatgagcag
      181 gattgtcttg gcatcctcga tctcgccacg cgcgatcatc tccagcgctt cctcgaggct
      241 cggttcggcg acatcgatgt cttcgccttc gttcgccaat ccgccgccgg ccgcgatcct
      301 gtcccgcgcg gaatagcgtg cggtgaagaa gctgattttc tcggcataga cgccggggct
      361 catataggca gagaagagga atgtcggcgc gccgatcttg acgccggttt cctccagaac
      421 ttccatcacg atgcgctccg gcgcctccgc gccctcgagc ttgccggcgc aggtttcgag
      481 caggctgtcg cggccttcga cgagatagac cgccggacgg aattgccgca cgagcaggat
      541 gcagcgccgc tccgggtcgt aaagcaggat cgttgcgccg ggcccggtat cgtagaattc
      601 gcgctcaatt tcttgcggct tgccatcata cttgcgccgc cagaaggtga cgcgttcgag
      661 cagcccaaag ccgcgcgaaa ccacttcgcg gcgaagaatt ttaaccgcgt cgttcatcgt
      721 caaagccgaa ttgttcgcgc aggatccgct cgtcgagcga atggcccgga tcgtgcagaa
      781 tgacgaggtt cgtcgcatgg tccattgcga tttcgacctt gatgacttcc ttgaactcga
      841 aatggtcggc gacggccgcg acgggccgct tgacggcctc aagcacgtcg agtgttacat
      901 gcgccgaatc gggcaggagt gcgccgcgcc agcgtcgcgg ccggaaggct gagatcggcg
      961 tcagcgccat gagcggcgaa ttcagcggca ggattggccc gtcgaccgaa agattatagg
     1021 ccgacgagcc agccggggtc gcaagcagca ccccatcggc gatgagctcc agaaggcgct
     1081 ccttgccgtc gaccgaaatg cggattttcg acgcctgata ggattgccgc agaaccgaga
     1141 cctcgttgat ggccatcgcg gtcgcgatct tgccgtgaat gtcggtggcg atcatcgcca
     1201 gcggatggat cacggaagat tccgcatcgt cgatccgctg gatcaagtcg tcttcgcggt
     1261 aatcgttcat cagaaagcca accgaaccgc ggttcatgcc gtagatcggc ttgccggtat
     1321 gcatgaagcg atgcagcgtc tgtagcatca gcccatcgcc gcccaacgcg acgacgacat
     1381 ccgccgtctc agggtcgctg tcgccataaa gttcgatcag acgcaagcgc gccgcctcgg
     1441 cctcgggcgt gttcgcgctg aggaaggcga tgcgcctccc gggtgcgttg gcgttcgtgt
     1501 cggtcatcat cgggctttcg tccaccgcat gttgaacacc gcagccggct gccgccggta
     1561 ttcgcctggc tcaataggcg gcgtagcttt tttcctcgat aatcgcgctt tgcaacaagg
     1621 cgttgatttc gcgctttaat accgcacggc ggtcgttggt ctgatagacc gaacgggcga
     1681 gggcgatgaa ttccgggccg aagtctttgg cgcgctcgtg gtcgcgaatg tcatcttcga
     1741 tgtgccataa ttcctgattg gcagctttta aggcggcggt cagctccgag agctgttcgc
     1801 ccggcgtgat ttgttcctgg agaacgccgc tcagaatctc atattcgcgg cgaatattga
     1861 cgcgtttggc cgtttcgttg atctgatcaa gcttgatctc aagaatcgtg atcttatcga
     1921 tcaactcgcc ggcggcgatt tcgacaaaaa tgggcatggg cagcccccct gcggatgcgt
     1981 ttcgctctta aacgctccca cgctcacccg caatacgttg aaatgcaacc cgcccgccta
     2041 tagaagcgcc tcgcgccctg agactggctt tgtgtggatc ttatgcccga ttttttgctt
     2101 gagctttttt ccgaggagat tcccgcccgc atgcaggtgc aggcggcgga agacctgaaa
     2161 aagctcgtta ccgaggtttt ggccgcgcag aatattttcg gcgaatcggc cggcagcttt
     2221 gttacgccgc gccgtctcgc gctgcacatc gagggtctgc cgccgcgcca gcgtgacacg
     2281 aaggaagttc gcaagggccc gcgcaccgat gcgccggctg cggcgatcaa cggttttctg
     2341 aaaagcgccg gcctcactaa tattgccgag gcgcagatcg agagcgatcc gaaaaagggc
     2401 gatttctatg tcgcgacgat cgaacggccg ggccgggcga ccggagaggt tctggcggac
     2461 atcctgccgc cgatcctgag caattttccc tggccgaagt cgatgcgctg gggcgcgcaa
     2521 tcggcgcagc cggaggcatt ccgctgggtc cggccgctcc attcaatcgt cgctaccttc
     2581 gggccggaga ctgaggagcc ggagatcgtc gcattctcca tcgctggtat tacggccggc
     2641 aatgttacgc acggccatcg ctttatggcg cccggcgcga ttcgcgtgcg ccgcttcgac
     2701 gactatgtgg cgtcgctcgg aaaggccaaa gtcgtcctcg atgcggcgcg acggcgcgac
     2761 attattctca atgatgcgcg taatctcgcc cacgcgcagg ggctggaact ggtcgaagac
     2821 gccggactgc tggacgaggt tgcggggctc gtcgaatggc cggtcgtcct catcggcgcc
     2881 ttcgacgcgg aatttctcgc gctgccgccg gaggtcatcc gcgccacgat ccgcgccaat
     2941 cagaaatgtt tcgtcctgcg cgatgcagcg accggcgcac tcgccaataa attcgtgctc
     3001 gtcgccaata tcgaagcgcc ggatggcgga acggcgattg cggccggcaa cgcccgtgtc
     3061 gtgcgggcgc ggctctcgga cgcgaagttt ttctacgaca ccgatcttca agtaaaactt
     3121 gaggatcgtc tgccaaagct ggaaaatatc gtcttccatg ccaagcttgg ctcgcaggcg
     3181 gagcgcgtca aaaggttggc cgaacttgcg cgttggctgg cgccgcacgt cggtgccgat
     3241 gccgagaagg ccgcccacgc cgcacgtctc gccaaagccg atctcgttac cgaaatggtc
     3301 ggcgaatttc ccgagctgca gggcctcatg ggccaatatt atgcccgcgc gcatggcgag
     3361 gcggcggatg tcgcggcggc gatcggcgaa cattatcgcc cgcaaggccc atcggattcg
     3421 atcccgaccg cgccggtttc gatcgcggtg gcgctggctg acaagttcga tacgctcgtt
     3481 ggattctttg ccatcgacga aaagccgacc ggcagcaagg acccttacgc cctgcgccgc
     3541 gcggcgctgg gcatcatccg catcatcctc gaaaataacc tgcggctgca tattacggca
     3601 gcgatagagc cggcacgcga aggctattca aatcttaaaa gcgttccgaa ttattttgca
     3661 tcaggcgtgc tcggattttt cgccgatcgc ctgaaagtct atctgcgcga caagggt
//
LOCUS       DASWOG010000702         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_218735,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000702 DASWOG010000000
VERSION     DASWOG010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218735"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VGB93_14375"
     CDS             complement(<1..1226)
                     /locus_tag="VGB93_14375"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0147302.1"
                     /translation="MSAATREPASDRSFGSASLRDENWLELPTFYPDALGFAARTSIA
                     ILLAYFVSFWAQIETPASAGICAALIAQPTQGMALSKAFYRVVGTIIGGVVALFLASQ
                     FPQSGLPLLIAFCLWLGLCSVVASLLRDFGAYGAVLCGYTVGIILLTDIDTPQNVFDT
                     AINRVAANQIGIAAVALVNLVFTRNVTYKRLASSLEARLVEVRLLARRILDRSDAPEP
                     LTYTKLEYSIVSLHSEVIYAAAELADGKTRSAGARNAIAGLVRMLVASRAIDRGLKAI
                     AIDADVDTILGQARRALDSTTGNLASYQAATPLEAFILERARGLICAHGLAADGLDVL
                     VNGANQKITAPQFTFYRDYWAAALSAVRTMIAAGLCATFCVISGWSGSSFMLIQLSAF
                     AALLGMQVNPTRSAIYA"
     gene            1586..>2542
                     /locus_tag="VGB93_14380"
     CDS             1586..>2542
                     /locus_tag="VGB93_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0147303.1"
                     /translation="MTVRKSISAYHQPAGGWGALKAVAEALDEQGIAVKGTTSLLRMN
                     QPEGFDCPGCAWPDPKHTSSFEFCENGAKAIAWEATAKRCAPEFFAAHTVTELDTWSD
                     YDLEMVGRVTHPMVYDAASDHYKPISWDDAFAVIGRHLNGLSNPNAADFYTSGRASNE
                     AAFLFQLFAREYGTNNFPDCSNMCHEATSVGLPESLGVGKGTVLLEDFDSTDAIFIFG
                     QNPGTNSPRMMTTLRDASRRGVPIISFNPFRERSLERFQAPQSPIVIVTLTSTPISES
                     LYQVRVGGDVAVLKGLMKAMIEADDAAIAVDAPRIVDADFING"
BASE COUNT          578 a          695 c          722 g          547 t
ORIGIN      
        1 gcgtatatgg ccgatctcgt gggattgact tgcatcccaa gtaacgctgc aaatgccgaa
       61 agctgaatga gcataaatga tgagccgctc cagcctgaga taacgcagaa cgtcgcgcaa
      121 agacctgccg ctatcattgt acggacggcg ctgagggcgg cagcccaata atcgcgataa
      181 aaggtaaatt gtggggctgt gattttttga ttcgcgccgt ttacaagaac atccagacca
      241 tccgcggcta gcccgtgcgc gcatatcaga ccgcgcgcgc gttccaatat gaaagcttcc
      301 agcggcgtcg ccgcttgata gcttgcgaga tttccagtgg tgctgtcgag agcgcgcctt
      361 gcctgcccta aaatcgtatc gacgtcggca tctattgcga tcgcctttag accgcggtcg
      421 attgcacggc tggccaccaa catgcggacc agtcctgcga tggcgtttcg ggcgccggcg
      481 cttcgcgtct tgccgtctgc aagctccgcc gctgcgtaaa taacttcgct gtgaagcgaa
      541 acgatcgaat attcgagctt tgtataagta aggggctcgg gtgcgtcgga tctatcgaga
      601 atccgccggg ccaataagcg gacttccacg agacgcgcct ccaatgacga agcaagccgt
      661 ttgtaggtta cgtttcgagt gaataccaga ttgacgagcg caacagcggc gattccaatt
      721 tgattggcgg cgacgcggtt tattgccgtg tcaaatacat tttgcggcgt atcgatgtcc
      781 gtcaagagaa tgattccgac ggtgtaaccg caaaggacgg caccataagc gccgaagtct
      841 cgcaacaaag atgcgacgac tgaacagaga ccgagccata agcagaatgc tatcagcagc
      901 gggagtccac tttgcggaaa ctggctcgcg aggaaaagag cgacaacacc accgataatc
      961 gtgccgacca cgcggtagaa ggctttggaa agcgccatgc cctgtgtcgg ctgagcgatg
     1021 agcgccgcac agattccggc tgacgcgggt gtttcaatct gagcccaaaa cgaaacaaaa
     1081 taggcaagca gaatggcgat tgatgtccga gccgcaaagc caagcgcatc cggataaaac
     1141 gttggcaatt caagccagtt ttcgtcgcgg agactggcag atccgaaact tctatcgctg
     1201 gcgggttcgc gggttgcggc tgacaaagtg tttcccccga tgtgagccga gctgagatta
     1261 aggcttttga aatgagtttc cactccgcca ggcgtcagcc aacaagaatc ctgcatgcgg
     1321 gcgcagcgcg tatgctgagt agttttttat atagcacgaa ctctcggctg agcgcgtcgg
     1381 acgaaatcga acccagattc tccgaacgcg catatgtgcg atggacaata ttcccgcacg
     1441 tgaattactt tatcgcatcg tgcaaaatcg gaatttttcg ggcgttttcg atcgtcatca
     1501 aactcaagag gtatgatagc ttcggggttg tctgaaatcc aagcttaccg tttggctcca
     1561 gccaagctca tgcagagggt tgcgcatgac cgtccgtaaa tcgatctccg cttatcacca
     1621 gccggctggc ggatggggcg cactcaaggc agttgccgag gcgctcgatg agcaaggcat
     1681 cgccgtcaag ggcaccacat cgcttttgcg gatgaatcag cccgagggct ttgattgccc
     1741 cggatgcgcg tggccagacc caaaacacac gtcatccttc gagttttgcg aaaacggcgc
     1801 gaaggcgatc gcgtgggagg ccacagcaaa acggtgtgcg ccggaattct ttgctgctca
     1861 cacggttacg gagctcgaca cgtggagcga ttatgatctc gaaatggtgg gacgtgtcac
     1921 tcacccgatg gtctacgatg ccgcttccga tcattataaa ccgatctcct gggacgatgc
     1981 ctttgccgtc attggccgtc atctgaacgg cctcagcaat ccaaacgccg cggactttta
     2041 tacatccggg cgcgcgtcca atgaggcggc atttctcttc cagctttttg cccgcgaata
     2101 cggcacgaat aatttccccg attgctcgaa catgtgccat gaggccacga gcgtgggcct
     2161 gcccgagtcg ctcggcgtcg gcaaaggcac ggtccttctc gaagacttcg attctaccga
     2221 cgctatcttt atcttcggtc agaatcccgg cacgaatagt ccgcgtatga tgacgacgct
     2281 gcgcgatgca tcgcggcggg gtgtacctat catctctttc aatccgtttc gggagcgctc
     2341 acttgaacgc ttccaggcgc cgcagagccc catcgtgatt gtgacactga cgtccacgcc
     2401 gatcagcgag agtctttatc aggtgcgtgt cggcggcgat gtcgcggtcc tcaaagggct
     2461 gatgaaggcg atgattgaag ccgatgatgc cgcgatcgcg gtcgatgcgc cgcgtatcgt
     2521 cgatgcggat ttcatcaacg ga
//
LOCUS       DASWOG010000703         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_36352,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000703 DASWOG010000000
VERSION     DASWOG010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36352"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1131
                     /locus_tag="VGB93_14385"
     CDS             241..1131
                     /locus_tag="VGB93_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEY0147304.1"
                     /translation="MRHLIRLLQRAAILALGAFAVWLIVFVIFRFAHQKLPLILAFSA
                     TYLAAAYVVLPYGIRMGLKILHRKHVPRYTITGDGLPGDPINLALVGDLAQLRAAFAA
                     AGWAGANRLDLKSSWGMVRAFVFNRPYPTAPFSPLYLFGRSHDIGFQQAIGDSPRKRH
                     HVRFWAKSLAQTQAEVTSARFWLNKDRPDENERVIWLGAATRDTGFSLTQMTFQITHA
                     TDSDTNAERAYIIGALTARGAIGDAILYKAGDHLPTKVNRYITDGDVAMAELAGERAG
                     FGAPRIRVTKPPMSGTDNKS"
     gene            1180..>1754
                     /locus_tag="VGB93_14390"
     CDS             1180..>1754
                     /locus_tag="VGB93_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0147305.1"
                     /translation="MMNRRDFTSGMAGVTAAAMMPKLALAANLPRAKNIVLVHGLFAD
                     GSCWSEVIAQLQPKGLNVTSVQNPLTTLDAAVASAQRVLDRQDGPTVLVGHSFSGMIV
                     TEAGVHPKVTALVYVAARAPDAGEDYPALAKKFPTPPASAGIVFDGDEGRLTEAAFLR
                     DFAGDLPEAQAKVLYAVQEPFHKALLLGKTK"
BASE COUNT          329 a          552 c          529 g          344 t
ORIGIN      
        1 gctttaccaa cgaagctaaa tacgaggccg agagatcgct cgcgcaccta actttgattg
       61 atagcgatcg attagtaaat ttgctgattg agtactacga aaagacagac gcggagactc
      121 gcaccctcgt tccgctccgc aaaacatatt ggcctgcttg atccgacact gaattctacg
      181 ttgactacgt aaatttgcag cagcgtggct ggcttgccgc cgcgaaccac gggaaagcgc
      241 atgcgacacc tgatccgcct gttgcagcgc gcggcgattt tggctctcgg cgccttcgcg
      301 gtctggctga tcgttttcgt catcttccgc ttcgcgcatc agaagctgcc gctgatcctc
      361 gcattcagcg cgacctatct cgccgcggct tatgtcgtcc tgccctatgg cattcgcatg
      421 ggcctcaaaa tcctgcatcg caaacatgtg ccgcgctaca cgatcaccgg cgacggcttg
      481 ccgggcgatc cgatcaatct ggcgctcgtc ggcgatctgg cgcagcttcg cgccgcgttt
      541 gccgccgccg gatgggcggg cgcgaatcgc ctcgatctga agtcttcgtg gggcatggtc
      601 cgggccttcg tcttcaacag accctatccg accgcgccgt tcagcccgct ctatctgttc
      661 ggccgcagcc atgacatcgg ttttcaacag gcgatcggcg acagtccgcg caagcgccat
      721 cacgtgcgct tctgggccaa gagcctcgcg cagacgcagg cggaggtcac gtcggcgcgt
      781 ttctggctca acaaggatcg gccggatgaa aacgagcgcg tgatctggct cggcgccgcc
      841 acgcgcgata ccggcttttc gctgacgcag atgaccttcc agatcaccca tgcgaccgat
      901 tccgatacca acgcggaacg ggcctatatc atcggcgcct tgaccgcgcg cggcgcgatc
      961 ggcgacgcca ttctctacaa ggccggcgat catctgccga cgaaggtgaa ccgatacatc
     1021 accgacggcg atgttgcgat ggcggaactg gcgggtgagc gggcgggatt tggcgcgcct
     1081 cgcatacgag tgacaaagcc acccatgtcc gggactgaca acaaatcgta gatttgccgc
     1141 tctatagcca agacggcact tcgatccgga gatattctta tgatgaatcg tcgcgatttc
     1201 acgagcggga tggcgggcgt gaccgcagcc gcaatgatgc ccaagctggc acttgcggcg
     1261 aaccttcccc gggcgaaaaa tatcgttctg gtgcatggcc tcttcgccga cggctcgtgc
     1321 tggtcggagg tgatcgcaca gcttcagccg aagggcctga acgtgacttc ggtccagaat
     1381 ccgctgacga cgctcgacgc cgccgtcgcg tcggcccaga gggtcctcga ccgccaggat
     1441 ggcccgacgg tgctcgttgg ccattccttc tccggcatga tcgtcaccga ggccggcgtc
     1501 catccgaaag tcacggcatt ggtctatgtc gcggcgcgcg cgcccgacgc gggcgaggat
     1561 tatccggcac tggcgaagaa atttccgacg ccgccggcct cggccggcat cgtcttcgac
     1621 ggcgatgagg gccggctgac ggaagcggcg ttcctgcggg atttcgccgg cgatttgccg
     1681 gaggcacaag ccaaggtgct ttatgccgtg caggagccgt tccataaggc gttgctactg
     1741 ggaaagacca agca
//
LOCUS       DASWOG010000704         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_53731,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000704 DASWOG010000000
VERSION     DASWOG010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53731"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="pal"
                     /locus_tag="VGB93_14395"
     CDS             <1..470
                     /gene="pal"
                     /locus_tag="VGB93_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0147306.1"
                     /translation="FKLAVALGAALAMAACAKNPGADSLAGGYGTATPGSPQDFSQNV
                     GDLVFFETDSTELTSTAQATLDKQATWLQQYGRYNFVVEGHADERGTREYNFALGARR
                     AERVKEYLTARGISGERIRTISYGKERPVAVCNDISCWSQNRRVVTDLNPGQS"
     gene            complement(655..2589)
                     /gene="ftsH"
                     /locus_tag="VGB93_14400"
     CDS             complement(655..2589)
                     /gene="ftsH"
                     /locus_tag="VGB93_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0147307.1"
                     /translation="MHPNFRNFALWVIIFLLVVALVMLFYNPSQRTAAQDSISFSEFL
                     SDVQGGQVREVTIVGNEVSGHLKNDKAFETYTPNDPSLVQSLVKNGVTINAKPPSDGN
                     SWLLTLLINGLPILAFVGMWIFVTRQMQGAGGKALGFGKSKAKLLTEAHGRITFEDVA
                     GVDEAKEDLQEIVEFLRDPQKFQRLGGRIPRGVLLVGPPGTGKTLLARAIAGEANVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDER
                     EQTLNQLLVEMDGFDANEGIILIAATNRPDVLDPALLRPGRFDRQIVVPNPDVIGRER
                     ILKVHVRKVPLSPDVDLKTVARGTPGFSGADLMNLVNEAALLAARRGKRIVMMSEFED
                     AKDKIMMGAERRTLVMTEQEKILTAYHEGGHAIVALNVPATDPVHKATIIPRGRALGM
                     VMQLPERDKLSMSYEQMTSRLAVLMGGRVSEEIIFGKDKVTSGAQSDIEQATKLARAM
                     VTRWGFSDQLGTVMYGENQEEVFLGYSMGKQNNISESTSQKIDAEVRRLVESGLADAT
                     RIITEKRQDLETLAKGLLEYETLTGEEIYGLLRGEKPIRDAGDEPPPAPAPRPSPVPA
                     SGRQPKAGPETGGLEPQPQT"
     gene            complement(2702..3793)
                     /gene="tilS"
                     /locus_tag="VGB93_14405"
     CDS             complement(2702..3793)
                     /gene="tilS"
                     /locus_tag="VGB93_14405"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0147308.1"
                     /translation="MRRLSRELSPTEPLSDASLASFFAPLAGRRALLAVSGGPDSVAL
                     MRLAAQWQGQGAPAGTSFFVATVDHGLRCESRAEAETVGRWAQSLGLPHQILAWQGAK
                     PRTRIQERARAARYTLLRAHARLIGADYLLTGHHADDQAETILFRLMRGSGIGGLAGM
                     QSMTEQDGVVLYRPLLGVPKKLLIDYCDACGRSYFRDPSNENSAFIRTRLRAFLPILD
                     EAGLDAAGLARLGHRAARAERALAAQADLARATLERTSTPDIVHISAASLRDLPEEIL
                     LRVVGAEIAALGSKPPRLERLESLAQDLYAALQLRKVWHRTLGGVKLSLDGEELTLRR
                     EALRRRGQVSASAHPASYDPAFWSGNAVS"
     gene            complement(3781..4890)
                     /gene="ybgF"
                     /locus_tag="VGB93_14410"
     CDS             complement(3781..4890)
                     /gene="ybgF"
                     /locus_tag="VGB93_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY0147309.1"
                     /translation="MTRLNTYVTALALAGAMILSVTSLSFAQNYGAPPDNVGGGGDSQ
                     GNGGSYAPDAAGGGSDASLIVRVEHLEDQIRQMTGQIQQMQFENRQLQDQLKKFEQDV
                     DFRLQDSRGGHLQRHSEMTPASPPPATDADSGSPPSDATTNPTDSADASAPPDTSPRH
                     GHSGDVFDPTADPNAPGAPKPLGAGESAPPPVADNGGGGAANSDPNAPLNLQSAYTPP
                     APSVPAGGVSTPAGTEFASTQPASPRAEFDAALGYFKQKDYDNAEHGFQSFLDKNPKS
                     RLTVEATYLLGESYFARGHAREAAEQFLKISTTYSASSRAPEAMLRLGESLRTLGAKE
                     QACATFSEVPRKYPNASAAVKAGAEREAKRSQCGA"
     gene            5130..>5619
                     /gene="glmM"
                     /locus_tag="VGB93_14415"
     CDS             5130..>5619
                     /gene="glmM"
                     /locus_tag="VGB93_14415"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601519.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0147310.1"
                     /translation="MAKHFGTDGIRGLANKVITPELAMKVAQATGILFQRGDHRHRAV
                     IGKDTRLSGYMIETAMVAGFTSVGVDVLLLGPMPTPAVAMLTHSMRADVGVMISASHN
                     PFEDNGIKLFGPDGYKLSDEVEARIEAILDGEQPVKLSKPGDLGRAMRVEGDRARYIE
                     FAK"
BASE COUNT         1071 a         1767 c         1712 g         1069 t
ORIGIN      
        1 ctttcaagct tgccgtagct ttaggcgctg ccctcgccat ggcggcctgc gccaaaaatc
       61 ccggggctga cagtctcgct ggcggctatg gcactgcgac gcctggttcg ccgcaggatt
      121 tcagccagaa tgtcggcgac ctcgttttct ttgaaacaga ttcgaccgag ctgacatcga
      181 ccgcgcaagc gacgctcgac aagcaggcga catggttgca gcaatacggc cgctataatt
      241 tcgttgtcga aggtcacgcc gacgagcgcg gcacacgcga atataacttc gcgctgggtg
      301 cgcggcgtgc cgaacgcgtc aaggaatacc tgaccgcgcg cggcatttcg ggcgaacgca
      361 tccgcacgat cagctacggc aaggagcgtc ccgttgcagt ctgcaacgat atttcgtgct
      421 ggtcgcaaaa ccgccgcgtt gtgacggacc tgaacccagg ccagtcgtaa gtctcgcgcg
      481 tattctttat tcgaaagcaa aaggccgatc cgtgaggacc ggccttttta tttgcgttca
      541 atcgcccgga taactttcag cggcggtgat cggagttcgg aaccgttcaa gcctgaacgt
      601 aaaaaagggc ggccaaagcc gccccttcaa tttcttatct gcgccgccga acgctcaggt
      661 ctgcggctgc ggctccagac cgccggtctc gggcccagcc ttcggctgcc ggccgctcgc
      721 cgggacaggc gatggacgcg gcgctggcgc cgggggcggc tcgtcaccgg catcgcggat
      781 gggcttttcg ccacgcaaga gaccgtaaat ctcttcgccg gtcagcgttt catattccag
      841 aagacccttg gccaacgtct cgaggtcctg acgcttctcg gtgataatgc gggtcgcgtc
      901 ggcgagaccg ctttcgacga gacggcgcac ttccgcatcg atcttctgcg aggtagattc
      961 ggaaatgttg ttctgcttgc ccatcgaata gccgaggaag acttcttcct ggttctcgcc
     1021 gtacatcact gtgccgagct ggtcggagaa gccccagcgc gtgaccatcg ctcgggcgag
     1081 tttcgtcgcc tgctcgatgt ccgattgcgc gccggacgtg accttgtcct tgccgaagat
     1141 gatctcctcc gagacacgtc cgcccatgag cactgcaaga cgcgaggtca tctgctcata
     1201 gctcatcgac agcttgtcgc gctcgggaag ctgcatgacc atgccgagcg cacgtccgcg
     1261 tggaatgatc gtcgctttat ggaccgggtc ggtcgccggc acattgagcg caacgatcgc
     1321 gtggccacct tcgtgatagg cggtcaggat tttctcctgt tccgtcatca ccaaagtacg
     1381 gcgctccgcg cccatcatga tcttgtcctt ggcgtcctcg aactcgctca tcatcacgat
     1441 gcgtttgccg cgccgcgcgg caagcagcgc cgcctcgttg acgagattca tgagatccgc
     1501 gccggagaag cccggcgtgc cgcgcgccac ggtcttcaga tcgacatcag gcgacagtgg
     1561 cactttgcgg acatgcacct tcaggatgcg ctcgcggccg atgacgtccg ggttcggcac
     1621 gacgatctgg cggtcgaaac ggcccggacg caacagcgcc gggtcgagca cgtccggccg
     1681 gttcgtcgcg gcaatgagga taatgccctc attggcgtca aagccgtcca tttcgacgag
     1741 caactggttc agtgtctgct cgcgctcgtc attgccgccg ccgaggccag cgccgcggtg
     1801 acggccgacg gcgtcgattt catcgatgaa gatgatgcag ggcgcgttct tcttcgcctg
     1861 ttcgaacatg tcgcgcaccc ggctcgcgcc gacgccgacg aacatttcga cgaagtcaga
     1921 gcccgagatc gtgaagaacg gcacgttcgc ttcgccagcg atggcgcggg cgagcaacgt
     1981 cttacccgtg ccgggggggc cgacgagcaa tacaccacgc ggaatccggc cgccgagccg
     2041 ctggaatttt tgcggatcgc gcagaaattc gacgatctcc tgcagatctt ccttggcctc
     2101 atcgacgcca gcaacatcct caaaggtgat gcgcccgtgg gcttcggtca gcagcttcgc
     2161 ctttgattta ccgaagccga gcgctttacc gcccgcgccc tgcatctgcc gggtgacgaa
     2221 aatccacatg ccgacaaagg caaggatcgg caggccgttg atcagcaggg tcaaaagcca
     2281 gctattgccg tcggacggcg gcttggcgtt gatcgtcacg ccattcttaa cgaggctctg
     2341 caccagcgac gggtcgttcg gcgtataggt ctcgaacgcc ttgtcattct tgagatggcc
     2401 cgacacttcg ttgccgacga tcgtcacttc gcggacttga ccgccctgga cgtcgctcaa
     2461 gaactcgctg aagctgatac tgtcctgcgc ggccgttcgc tggctcgggt tgtagaacaa
     2521 catcacgagc gcgactacga gcagaaagat gatgacccag agtgcaaaat tcctgaaatt
     2581 cggatgcata ttctactcgc gacaggctgg ctccgaccct ctgagacagc ccgttaaagg
     2641 aggcgctccc tggagcgcgt tggcctaatg taggcgtcgc cgtaaggctt gccaagggaa
     2701 cttaagatac ggcattgcca ctccaaaaag ctgggtcata tgacgccgga tgagcggagg
     2761 cgctgacctg gccgcgccgc cgaagcgcct cgcgccgaag ggtcaactcc tcgccgtcga
     2821 gcgataactt cacaccgccg agcgtacgat gccagacctt acgtaattgc aatgctgcat
     2881 acaggtcttg cgccaaactt tcgagccgtt cgagacgcgg cggcttgctg ccgagagccg
     2941 cgatttcggc gccgaccaca cgcagcaaaa tctcttccgg cagatcgcgc aagctcgcgg
     3001 cggaaatatg gacaatatcc ggagttgagg tccgctcgag cgttgcccgc gcgagatcgg
     3061 cctgggctgc taaagcgcgt tctgctcgcg ccgcacggtg gccgagacgg gccaaaccag
     3121 cggcatccag gcctgcctca tcaagaatag gaagaaaagc acgcaacctc gtgcgtataa
     3181 aagcagaatt ttcgtttgaa ggatcacgaa aataggatcg accgcacgcg tcacaataat
     3241 caatcagcag ctttttcggc acgccaagca acggccggta gaggacgact ccatcctgtt
     3301 ccgtcatgct ttgcatgccg gcgaggccgc caataccgct gccccgcatc agccgaaata
     3361 gaatcgtttc cgcctgatca tcagcgtgat gtcccgtcag caaataatcc gcgccaatca
     3421 accgggcgtg cgcccgcagc agcgtatagc gcgccgcgcg agcccgctct tggatgcgtg
     3481 tgcgaggctt cgcaccctgc caagcgagga tttgatgcgg caggccgagc gactgcgccc
     3541 aacgaccgac cgtctcggcc tccgcccgcg attcgcagcg cagaccatga tcgacggtgg
     3601 cgacgaaaaa gcttgttccg gcgggtgcgc cctgcccctg ccattgcgcc gcgagccgca
     3661 tcagcgcgac ggaatccgga ccgcctgaca ccgcgagcaa ggcgcgtcga ccggcaagcg
     3721 gcgcgaaaaa cgacgcgagg ctcgcgtcgc tcaggggttc cgtcggcgac agttccctgc
     3781 tcaagcgccg cactggctgc gcttggcctc ccgttcagcc cccgctttga cggcggctga
     3841 agcgtttgga tatttgcgcg gcacttcgct gaaggtggcg caggcttgtt ccttggcgcc
     3901 gagggtgcgc agggattcgc cgagccgcaa catcgcctcc ggcgcccgcg acgaggccga
     3961 ataagtcgtc gaaatcttca agaattgctc ggcagcctca cgcgcgtgcc cccgggcgaa
     4021 ataggattcg cccaagagat acgtcgcctc gacggtcagg cgcgacttcg ggtttttgtc
     4081 gagaaacgac tgaaagccat gctcggcgtt gtcgtaatcc ttctgcttga aatagccgag
     4141 agcggcgtcg aactccgcac gtggcgaagc cggctgcgtg ctggcgaact ccgtgccggc
     4201 gggcgtcgag actccgccgg ccggcacgct gggcgccggt ggcgtatagg cgctctgcag
     4261 attgagcggg gcattcggat cgctgttggc cgctccgcca ccgccgttgt cggcaacggg
     4321 cggcggcgcg ctctcgccag cgccgagggg cttcggcgcg ccgggagcgt tgggatcagc
     4381 cgtcggatcg aagacatcgc cgctatgccc atgccgcggc gacgtatcag gtggcgcgct
     4441 ggcgtcggcc gaatcggtgg ggttcgtggt ggcgtcggaa gggggagatc cgctgtctgc
     4501 gtctgttgcc ggcggcggcg aggccggcgt catctcactg tgacgctgga ggtggccgcc
     4561 acggctatcc tggagccgga aatcaacgtc ttgctcgaat ttcttaagct gatcttgaag
     4621 ctgccggttc tcgaactgca tttgctggat ctgtccggtc atctgccgga tttgatcctc
     4681 aaggtgctcg acgcggacga tcaagctggc gtccgaaccg ccgccggcag catccggcgc
     4741 ataagagccg ccgttgccct gcgaatcacc cccgccgccg acattgtccg gcggcgcgcc
     4801 atagttttgc gcgaagctca gtgaggttac gctcaggatc atggcgccag ccagcgccag
     4861 cgccgtcaca taggtgttca atcgagtcat gctaaaaaaa tccagattac ccaaacacat
     4921 cataaggcac acttcgacca aagttaggtt ttcgagaaga aaatcatcag gagcggccgg
     4981 cggcttcccc atcgtgagtt tgtaacgccc aaccgatttt gcgtaatatc gggcttccgg
     5041 ctacaatcgg gacaatgtct cgcgagcctt ttaaggccgt tttcgccgcg accggctcgt
     5101 caattcagcg aatgcaggag caccagggct tggcaaagca tttcgggacc gatggaattc
     5161 gcggcctcgc caataaggtt atcacgcccg aactggcgat gaaggtggcg caggccacgg
     5221 gcatcctgtt tcagcgcggc gaccatcgcc atcgcgccgt gatcggcaag gacacaaggc
     5281 tttccggcta tatgatcgag accgccatgg tcgctggctt tacctccgtc ggcgtcgatg
     5341 tgctgctgct cggtccgatg ccgacaccgg ccgtcgccat gctgacccat tcgatgcgcg
     5401 ccgatgtagg ggtgatgatt tccgcctccc acaacccgtt cgaagacaac ggcatcaaat
     5461 tgttcggccc ggacggctac aagctctcgg acgaggtcga ggcgaggatc gaagccatcc
     5521 tcgatggcga gcagcccgtg aaactgtcaa agcccggcga tctgggccgc gccatgcgcg
     5581 tcgagggcga ccgcgcccgc tatatcgaat tcgccaagc
//
LOCUS       DASWOG010000705         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_72193,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000705 DASWOG010000000
VERSION     DASWOG010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72193"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGB93_14420"
     CDS             <1..587
                     /locus_tag="VGB93_14420"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEY0147311.1"
                     /translation="PLLARFFGRAAGHSAPLAAGTLATPGLLAVLMALAALLSWILMR
                     RRIPHAWLLGAMAIGAGAALTTATRFSVPTWSLDLAQILIGTSLGARLRRKALRNHRR
                     LLPATIASTALLLAGNLVLGLILLALTGTGLTTAILSTSPGGLAEMSLTAIALKADVS
                     LVVAFHLARTLIVALSSLPLYAATRRLSGHKASP"
     gene            complement(561..1562)
                     /locus_tag="VGB93_14425"
     CDS             complement(561..1562)
                     /locus_tag="VGB93_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0147312.1"
                     /translation="MIDVQQLECFCAVVRSGSFSKAAAAMSIGQPGLSRHVRRLEEQL
                     GSKLLYRNGRGVALTPAGSQFHATVSVLIAQLKEAYDSAAQDSDAPAGHVTLGLPTSM
                     AGLVGAPVLRALKVRYPHIELSLIDGFSGHIHGWLVSGRIDMAILHDARHAPAIAVEP
                     LSSEDLFLIGPKTPPAALVRGNETIIALRDIATLPLAIPGPDHGLRRQIDRVSAEAGV
                     KLSVESNVDSLTVIKELINDGGIYTILPIGCVYQEALAGTLRVCRIVDPPIVNTFVLA
                     AAQNRPFTAAMRTVRNALRDQIALAFTPEGGRKPADKPQRPANVVQIAASRRRLVSA"
     gene            complement(1702..2148)
                     /locus_tag="VGB93_14430"
     CDS             complement(1702..2148)
                     /locus_tag="VGB93_14430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0147313.1"
                     /translation="MTSQGVFTAREFRDALGLFPTGVAVVTALSGAGEKLGVTVSSFS
                     SISLDPPLVSFGLAKSAHSFDDWVAAPHFAINVLTEEQEHLSTKFARALSDKWSGLSP
                     RQGAAVDVPLLDEALAWFECAKFAQHDAGDHLIVLGRIMSFARTPH"
     gene            complement(2153..3646)
                     /locus_tag="VGB93_14435"
     CDS             complement(2153..3646)
                     /locus_tag="VGB93_14435"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0147314.1"
                     /translation="MTVAEFSKAVPASTTRDLRTGAEFLQGLDDGRAVYFDGERVKNV
                     ATHPAFSGAAKSSARLFDIAADPANRERMTTTSPSGKPMWRAYQIPRTHADLRAKRLA
                     SEMWSEASFGLIGRSPDHVSGFFTGFAAVPEVFAEGGKQFADNVVSFYEHLRDNHLWA
                     TYAIVPPQIDRSKPAHQQKDPTLYAGVVKETDGGIVISGAQQLASAGVYSDYIYLSCI
                     HPLKAGDENYAIGVAIPLNAEGLRLYPRRPFAAQASNTFDHPLSSRFDESDCLVVLDN
                     VVVPWERVFVYRNLQVCWNQWWKTPSHLYGNLQAQARYATKLRFMIGLAKRINEMTGN
                     DGVPPVMVEMGELAAFVSIVETMLEAQEVKAHIDEKGIVWPSRQALYAVMALQSEINP
                     RMLEIIRELSGAAMITLPSSVKDLESPETANDMERFMQSATVGARERVQLLRMAWDFI
                     GSEFGNRSAQYEKFYGGASFLVKQNMFRAYDFSRATELVDTALALPD"
     gene            3897..4439
                     /locus_tag="VGB93_14440"
     CDS             3897..4439
                     /locus_tag="VGB93_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0147315.1"
                     /translation="MPPVTISNETFWVDGRDDWLRETLYLMVLCLNQLHTFREAFGRA
                     MDLTSPQFAVLMGTAYTQGAEGVSIGTLATHVHLAPTHVTTEVGRLIRLGLLQKRPNN
                     EDRRSVLVRLSPQGEKAIRAVVPLVRSVNDQLFDSVTRDELQSILQFFRRFATNSEIA
                     LTRINSTKRPAKRRSAKTPD"
     gene            complement(4466..>4774)
                     /locus_tag="VGB93_14445"
     CDS             complement(4466..>4774)
                     /locus_tag="VGB93_14445"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEY0147316.1"
                     /translation="ANALLTTVFANPIRDAIGGGDAWISSVTKVAAPGTMIDVPMNSI
                     DDVYIRSHYGTMSLVLPDAPLPDEVALIFCFATGGRLNARVGGITVEEVRKQRATAKV
                     "
BASE COUNT          993 a         1496 c         1393 g          892 t
ORIGIN      
        1 tgccgctttt ggcaagattc ttcggccgcg ccgcgggcca cagcgcgcct ctcgcagccg
       61 gaacgctggc aacgcccggc ctcctcgccg tccttatggc ccttgccgcg ctcctgtcct
      121 ggattctcat gcggcggcgc attccacatg cctggcttct cggtgcgatg gccatcggcg
      181 ccggcgccgc gttgacaaca gcaacgcgat ttagcgtccc gacctggagc ctcgatctcg
      241 cgcagatctt gatcggcacc tcgctggggg cgcgcttgcg gcgcaaggcg ctacgcaacc
      301 atcgccgcct gctcccagcc accattgcct ctacagcgct tctcctggcc ggcaatctcg
      361 tgctcggtct gattctcctg gcgctgacag gcaccgggct gacgacggcc attctgagta
      421 cgtcgcccgg cggccttgcg gaaatgagcc tgacggcaat cgcgctgaag gccgacgtca
      481 gcctcgtcgt ggccttccat ctggcgcgca cgttgattgt ggcgctcagc agtttgccgc
      541 tttacgcagc gacgcgcagg ctaagcggac acaaggcgtc gccttgaagc cgcgatctgc
      601 accacattcg cgggccgctg cggtttatcg gcgggcttcc ggccgccctc gggcgtaaaa
      661 gcgagtgcaa tctgatcgcg taacgcattt cttacggtgc gcatcgccgc cgtaaaaggc
      721 cggttttgcg ccgccgccag aacaaacgta ttaacgatcg gcggatcgac aatacggcag
      781 actcgcaacg tgccggcaag cgcttcctga tacacgcagc cgatgggcag aatcgtgtaa
      841 atgcccccgt cgttgatcag ctctttaatg accgtcagcg aatcgacgtt gctttcaacg
      901 ctcaatttca cgcctgcttc cgcactgacg cgatcgattt gccggcgcag gccgtgatcc
      961 gggccgggga tcgctagcgg cagagtcgcg atatcgcgca gcgcaattat agtttcgttg
     1021 ccgcgcacga gtgcggccgg cggagtcttc ggcccgatga ggaacaggtc ctccgacgaa
     1081 agcggttcga ctgctatggc tggcgcgtga cgcgcatcgt gtaaaatcgc catatcgatc
     1141 cggccggaca caagccaccc gtggatatgc ccggagaagc catcgatcag gctgagctcg
     1201 atatgcggat agcgcacttt taacgcgcgc aagaccggag cgccgaccag tccggccatc
     1261 gaggtcggca ggccaagcgt cacatggccc gcaggcgcat cgctatcttg tgccgccgag
     1321 tcataggcct ctttgagctg agcgatgaga accgaaacgg tcgcgtgaaa ttggctgccg
     1381 gccggggtca atgcaacgcc acgtccgttg cggtaaagta atttcgagcc aagctgttct
     1441 tcgaggcggc gcacatgccg gctcagaccc ggctgaccga tcgacatcgc cgcggccgcc
     1501 ttggaaaagc tgccgctgcg gacaacggcg caaaagcatt caagttgctg gacgtctatc
     1561 attttcggca ctcgcacata ttcgcaaccg acgcgaaata cgtgccaagt tccggaatga
     1621 cgcctattcc gccggcttca ccgattcggg cccatgcagc cgatgatagg cgctcttgaa
     1681 aaagacgagt ggccggcgtc ctcaatgcgg cgtgcgcgcg aaagacatta tgcgcccgag
     1741 aacaatcaga tgatcgccgg catcgtgttg cgcgaacttg gcgcattcga accacgccag
     1801 tgcttcgtcc agtagcggca cgtcaaccgc ggccccctgg cgcggcgaca ggccgctcca
     1861 tttgtcgctc agagcacgtg cgaatttcgt cgaaagatgt tcctgctctt cggtcagcac
     1921 attgatggcg aaatgcggcg ctgccaccca atcgtcaaat gaatgcgccg actttgccag
     1981 cccgaaagag acaagcggcg gatcgagcga gatggacgag aacgaactca ccgtcacgcc
     2041 aagcttttcc cccgcgccgc tgagcgccgt gacgaccgcg acgcccgtcg ggaaaaggcc
     2101 aagcgcatca cgaaattcgc gtgcggtaaa gacgccctgc gaggtcatat gcttagtccg
     2161 gcaacgcgag cgcagtatcg acaagctccg tcgcacgaga aaaatcatag gcgcggaaca
     2221 tgttctgctt cacgaggaac gacgcaccgc catagaactt ctcatattgt gccgagcggt
     2281 tgccaaattc cgaaccgatg aaatcccaag ccatgcgcag gagctggacc cgctcgcgcg
     2341 cgccgacagt agcgctttgc atgaagcgct ccatgtcgtt cgcggtctcg gggctttcga
     2401 gatccttcac cgaggacggc aacgtgatca tcgccgcgcc gctcaattcg cgaatgatct
     2461 cgagcatgcg cggattgatc tccgattgca gcgccatgac ggcgtagagc gcctgccgcg
     2521 atggccagac gatgcccttt tcgtcgatgt gcgccttcac ctcctgcgct tcgagcatcg
     2581 tctcgacgat cgaaacaaac gccgcaagct cacccatttc caccatcacc ggcggcacgc
     2641 catcgtttcc ggtcatctcg ttgatccgct tggcgagccc gatcatgaag cgcagcttgg
     2701 tcgcgtaccg cgcttgcgcc tgaaggttgc catagagatg cgacggcgtc ttccaccatt
     2761 gattccagca gacctgaagg ttgcggtaaa cgaacacgcg ctcccaggga acgacgacgt
     2821 tatccagcac gacaagacag tcggactcgt cgaaacggct cgacaaagga tgatcaaacg
     2881 tattcgacgc ttgcgccgcg aaggggcgcc gcgggtaaag ccgtaggccc tccgcgttca
     2941 acggaatcgc gacgccgata gcataatttt catcgcctgc cttcagcggg tggatgcaac
     3001 tcagatagat ataatcggaa tagactccag cggacgcgag ttgctgcgcg ccggaaatga
     3061 cgatgccgcc gtcggtttcc ttcaccacac cggcataaag cgtcggatct ttttgctgat
     3121 gtgcgggctt cgagcggtcg atctgcggcg gcacgatcgc ataagtcgcc caaagatgat
     3181 tgtcgcgcag atgctcataa aacgagacga cattgtcggc gaattgcttg ccgccttcag
     3241 caaaaacctc aggaacggcg gcgaaaccgg taaaaaatcc agagacgtga tcgggcgagc
     3301 gcccgataag cccgaacgac gcttccgacc acatttctga ggccagacgc ttggcacgca
     3361 ggtccgcatg cgtccggggg atctgatagg cgcgccacat cggcttcccc gacggcgacg
     3421 tcgtggtcat gcgttcgcga ttggcgggat cggcagcgat gtcgaagaga cgcgcggagg
     3481 attttgccgc accggaaaac gctgggtgcg tcgcaacgtt tttcacgcgc tccccgtcga
     3541 aatagacggc gcgcccgtca tcgagcccct gcaaaaattc cgcgcccgta cgcagatcac
     3601 gcgttgtgga ggccggaacg gctttcgaaa actcagcgac ggtcatcaag ctctcctgag
     3661 ggcgccgcgc acgagcggca cgtccacgcg ttaacaatat atacatattg tacatattta
     3721 ggcccgtcaa tgcaaatcgc actgttgaat tacgccgacg ctcaacgtta aacagtcgca
     3781 gtacgcaccg ctgaaggtcg attcagcgac ctgacgaggg ccacttgacc agaaataagc
     3841 aagaagcggt ccgttgcgac gccgcaccac gacaaaggcc aaagcagcct cttttcgtgc
     3901 cgcctgtcac catctcgaac gaaacgttct gggtcgatgg ccgcgacgat tggctgcgcg
     3961 agacgcttta tctgatggtt ttatgcctga accagcttca cacattccgt gaagccttcg
     4021 gccgggcaat ggatctgacg agcccgcaat tcgccgtcct gatgggcacc gcctatacgc
     4081 aaggcgcgga gggcgtgagc atcgggacgc tcgcaacgca tgtgcatctt gcgccgaccc
     4141 atgtgacgac cgaagtcggc aggttgataa ggctgggtct gcttcaaaag cgcccgaaca
     4201 acgaagaccg tcgcagcgtc ctggtccggt tgtcaccgca aggcgaaaaa gccattcgcg
     4261 ccgtcgtgcc tctcgtgcgt tcggtcaacg atcagctttt cgacagtgtg acacgcgacg
     4321 aattgcagtc gatcctgcaa ttcttccggc gcttcgcgac caatagtgaa attgccttga
     4381 ccaggatcaa ttcgacaaag agaccagcaa aacggcgaag cgccaaaacg ccggattgat
     4441 ctcagcccgg cgacagatgt cggcgttaga ccttggccgt cgcgcgttgc ttccgcactt
     4501 cttccaccgt gatgccgccg acgcgcgcat tcagccggcc gcctgtcgcg aaacagaaga
     4561 tcaaggcgac ctcgtcgggt aacggcgcgt cgggcaggac caggctcatc gtcccgtaat
     4621 gcgagcggat atagacgtcg tcgatggagt tcatcggcac atcgatcatc gtaccgggtg
     4681 cggcaacctt ggtcaccgac gaaatccagg cgtcgccgcc gccgatcgca tcgcgaatgg
     4741 ggttggcgaa aaccgtggtc agcagcgcat tcgc
//
LOCUS       DASWOG010000706         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_72197,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000706 DASWOG010000000
VERSION     DASWOG010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72197"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /gene="lpxD"
                     /locus_tag="VGB93_14450"
     CDS             <1..574
                     /gene="lpxD"
                     /locus_tag="VGB93_14450"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0147317.1"
                     /translation="GPNVRIGRGCAIGPGAVIANALIGNRVIIHAGVKIGQDGFGFIA
                     GPNSHLKVPQIGRVIVQDDVEIGANSAIDRGATRDTVVGEGTKIGNLVQIGHNVRIGR
                     HCLIVAQAGISGSTELGDFVGVGGQAGFAEHLEIGNRAQIAAQSGVNDDLPAKARVGG
                     TPARPLRTWLRGETWLAREAAERHRHGSTE"
     gene            584..1066
                     /gene="fabZ"
                     /locus_tag="VGB93_14455"
     CDS             584..1066
                     /gene="fabZ"
                     /locus_tag="VGB93_14455"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0147318.1"
                     /translation="MDEKVANVLEAYDIERLLKSLPHRYPFLLVDRIVEANGDESCIG
                     IKNVSVNEPQFQGHFPERPVMPGVLMIEGMAQTAGALCINAIAGGVKPPLVYFMTIEK
                     AKFRKPVLPGDRIEYHMQKLNRRRTIWWYSGRALVDGVLVCEAEISAMLVLDEPAKAK
                     "
     gene            complement(1082..1705)
                     /locus_tag="VGB93_14460"
     CDS             complement(1082..1705)
                     /locus_tag="VGB93_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEY0147319.1"
                     /translation="MGINKIIRTFRVAMALAGLTLANSQSQALDLRASTATLTGPTSV
                     PYGWVDLCGREPQECRQPVLAAADLDLTPAMWRLLNSVNRHVNSTIVPISNLDHWGTL
                     LDHWDYPKDGKGDCKIYALWKRKLLMDAGLPRQALLMTIVRDLDGEGHTILTVKTNRG
                     EFILDNLRDDIRPWDATGYHFLKRQSQEDPNIWVGIATANSRVSSLN"
     gene            2038..>2320
                     /locus_tag="VGB93_14465"
     CDS             2038..>2320
                     /locus_tag="VGB93_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0147320.1"
                     /translation="MIKAVVVHEVSGPNVMHLEEIEMPKPRPGEALVRNHAVGLNYID
                     TYFRSGAYAAQVPFVPGNEAAGEVIELGKGVKDVKIGDRVAYLTHLGGYA"
BASE COUNT          452 a          632 c          765 g          471 t
ORIGIN      
        1 cggcccgaat gtgcggatcg ggcgcggctg cgccatcggg cccggcgcgg tgatcgccaa
       61 cgcgctgatc ggcaacaggg tcatcatcca cgccggggtc aagatcggtc aggacgggtt
      121 cggctttatc gccgggccga acagccatct gaaagtgccg cagatcggcc gggtcatcgt
      181 gcaagacgat gtcgagattg gtgccaattc cgcgattgat cggggcgcga cgcgcgatac
      241 ggttgtcggt gagggcacga agatcggtaa tctcgtccag atcggccata acgtgcggat
      301 cggtcggcat tgtttgattg tcgcgcaggc gggcatttcc ggctcaacgg aattgggcga
      361 tttcgttggt gtcgggggcc aggcgggctt tgcggaacat ctcgaaattg gcaatcgcgc
      421 gcagatcgcg gcgcaatcgg gcgtcaacga tgaccttccg gccaaggcgc gggtgggagg
      481 aacgccggcg cgcccgcttc gcacatggtt gcggggcgag acttggcttg cgcgggaagc
      541 tgcggagcgg cataggcatg gttcaacgga gtgaaaccag ccgatggatg agaaggtggc
      601 aaacgttctc gaagcctatg atatcgagcg gctcctgaaa tcgctgccgc atcgttatcc
      661 gtttttgctg gtcgatcgga tcgtcgaagc gaatggtgat gagtcctgca tcgggatcaa
      721 gaacgtcagt gtgaacgaac cgcagttcca gggccatttt cccgagcgac ccgtcatgcc
      781 tggcgtgctg atgatcgagg gcatggcgca gaccgcgggc gcgctttgca tcaacgccat
      841 tgctggcggg gtgaagccgc cgctggttta tttcatgacg atcgaaaagg cgaagttccg
      901 caagccggtc ctgccgggcg atcgaatcga atatcacatg cagaagctca atcggcgtcg
      961 taccatctgg tggtacagcg gccgcgctct ggtcgatggc gtgcttgtct gcgaggcaga
     1021 gatttcggcc atgctcgtgc tcgacgagcc ggcgaaggcg aagtagctcg cgttcccgcg
     1081 attaattgag gctggaaacc cgcgagttcg ctgtggcgat tccgacccag atgttggggt
     1141 cttcctgcga ctggcgcttc aggaaatgat agccggtcgc atcccagggg cggatgtcgt
     1201 cgcgaagatt atcgagaatg aactcgccgc ggttggtctt gacggtcagg attgtgtgac
     1261 cttcgccatc gaggtcgcga acgatggtca tcagcagcgc ctggcgcggc agtcccgcat
     1321 ccatgaggag cttgcgcttc cacagcgcat aaatcttgca atcgcctttg ccgtctttcg
     1381 gatagtccca atgatcgagc agcgtgcccc aatgatcgag attggagatc ggcacgatcg
     1441 tgctgttgac gtgacgattg acggaattca acaagcgcca catggcgggc gtgagatcga
     1501 gatcggcggc ggccaggact ggctggcggc attcctgggg ttcacggccg cacaaatcga
     1561 cccagccata gggcacagac gtgggcccgg tcagcgtcgc agtgctcgcc cgaaggtcca
     1621 gagcctggct ctgggaattg gcaagcgtta gccctgcgag agccatagct acccggaacg
     1681 tccggatgat cttgttaatg cccattatca gccccaccga aggcggggcg atccccgcgc
     1741 tttcatgggg cgaatttgac gggccgccct ttgccgggct cagaaacaaa aagctagaat
     1801 tttatcgatc ggccggaatt ttcggcggct ctcggcggtt ggtgaatcaa ccgcgaacgc
     1861 atgtggtttc tgatttgttt gcagcccacg cgccgaagcg agcgtgagtg tggccgtgcg
     1921 cccatacaaa ttatcgtggc tggatatccg gcgtcgcggt cattccgcgt gctcaatcgt
     1981 cttgccgaat cttgttttga gcaagaatgt cgggccggaa attcgaggaa gagtgcaatg
     2041 atcaaagcag ttgtcgtgca tgaggtcagc ggacccaacg tcatgcatct tgaagaaatt
     2101 gagatgccga agccccggcc gggtgaggct ctggtgcgca atcatgccgt cggcctgaac
     2161 tatatcgaca cctattttcg ctcgggcgct tatgcggcgc aggtgccgtt cgttcccggc
     2221 aacgaggcgg ccggcgaagt gatcgagctc ggcaagggcg tgaaagacgt caagatcggc
     2281 gaccgcgtcg cctatctcac acatcttggc ggctatgccg
//
LOCUS       DASWOG010000707         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_146618,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000707 DASWOG010000000
VERSION     DASWOG010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146618"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VGB93_14470"
     CDS             <1..683
                     /locus_tag="VGB93_14470"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0147321.1"
                     /translation="LAVPHGLAIKFHLKDGSETDIVINSLKFFPVATGEDFRDLLLAI
                     AASPPNAPQPTKLALFVKTHPRVALASATVHQPDSLADEQYNGVDAFVFVDKSGKKQA
                     FRYIAAPEKLVHISDEEAAKKPPNYLFDEIATRVAKAPVTFHLQAQLAAPGDVTKDPT
                     IPWAATNKVVDLGVLTINKIDANSLEEQKKLLFLPGALIPGIEESDDTLVDIRDGAYA
                     VSFGRRSQ"
     gene            865..1362
                     /locus_tag="VGB93_14475"
     CDS             865..1362
                     /locus_tag="VGB93_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0147322.1"
                     /translation="MAPDPKIKLRPATPADAGLIFAFVGELAAFEHLSHEVLTDEAGL
                     RNALFGDNPRVFAEIVEYDGAAAGFILWFYTFSSFAGRHGIWIEDLYVRETFRGKGLG
                     AAMLHAIARRCVEEKLARLEWSVLDWNENAIRFYRGAGAQLKSEWTICRMEGEALADF
                     ADGHT"
     gene            1359..1901
                     /locus_tag="VGB93_14480"
     CDS             1359..1901
                     /locus_tag="VGB93_14480"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0147323.1"
                     /translation="MSLTLVGVVAVADNGVIGQGNALPWHISADLKRFRALTLGKPLI
                     MGRKTFAAIGRALPGRETMVVTRDAKFAPPAGVFTATTIDAALQLATTRADVLGASEI
                     IVAGGGEIYAALLDRIDLLHMTYVHCTPEGDAFFPPIEWSHWREVFREDHLSETGGDA
                     GYTWADYIRTGSLSLAPKAL"
     gene            1928..>2505
                     /locus_tag="VGB93_14485"
     CDS             1928..>2505
                     /locus_tag="VGB93_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0147324.1"
                     /translation="MPWSNDGKDGGSWKPENPGPWGQPQAPNPVNFEGWVRLAQARLQ
                     GWLPGGGLGGRALAVLGLLGVLLWLLTGCYTIGPNEVGLNMIFGRYTGKTTSGLNYNL
                     PYPIGSVQKLAVTDRNTTDIGFISRMDDRTGEQATFDLPEESLMLTDDQNIADVKFVV
                     IWQIDPSHPEDYAFNVADPDDTVKAVAESAMRA"
BASE COUNT          494 a          739 c          761 g          511 t
ORIGIN      
        1 aattggctgt accgcacggc cttgcgatca aattccatct gaaggacggc agcgagacgg
       61 atattgtcat caattcgctg aaatttttcc cggtcgcgac gggcgaggat ttccgtgatc
      121 ttctgctcgc cattgcggcc agcccgccga acgcgccgca gccgacgaag cttgcgctct
      181 tcgtcaagac gcatccgcgt gtggcgttag catcggcgac ggtacaccag ccggacagcc
      241 tcgccgacga acaatataac ggcgtcgatg cgttcgtttt tgtcgacaag agcggcaaga
      301 aacaggcgtt ccgctatatc gccgcgccgg agaaactcgt tcacatcagc gatgaagaag
      361 ctgccaaaaa gccgccgaat tatctcttcg acgagatcgc cacgcgcgtt gccaaggcgc
      421 ctgtcacttt ccatctgcaa gcgcagctcg cggcgccggg cgatgttacc aaggacccga
      481 cgatcccctg ggcggcgacc aacaaagtgg ttgatctggg cgtcctgacg atcaacaaga
      541 tcgacgccaa cagtctcgaa gagcagaaga agctgctgtt cctgcccggg gcgttgatcc
      601 cggggatcga ggaatcggac gatacattgg tcgacatccg cgacggcgcc tatgccgtct
      661 cgttcgggcg gcgcagccaa tagatcacga tgattttgga ttaacccaac tcgggcttgc
      721 ccgagttggg cctcttgaag ttcaactcgg ctttgccgta cttgaacgat ctaaaatcat
      781 gaacgtgatc gattccaaaa acttagagcg ggatttgcgc tctaggttgc gcggaacgcg
      841 cttctggcgc aaaacctcgc cgccatggcg ccggacccga aaatcaagct acgccccgcg
      901 acgcccgccg acgcggggct gatctttgcc tttgtcggcg agctcgccgc cttcgaacat
      961 ttgtcgcatg aagtcctgac cgacgaagcg ggtctcagga acgctttgtt cggcgacaat
     1021 ccgcgcgtct tcgccgaaat tgtcgaatac gacggcgccg cggccgggtt cattctctgg
     1081 ttttatacgt tttcgagttt tgccgggcgt cacggcatct ggatcgaaga tctgtacgtg
     1141 cgcgagacct ttcgcggcaa aggtctgggt gcggcgatgc ttcacgccat cgcgcggcgc
     1201 tgcgtcgaag agaaacttgc ccggcttgaa tggtcggtcc tggattggaa cgagaatgcg
     1261 atccgcttct atcgcggcgc cggcgcgcaa ttgaagtccg aatggacgat ctgccgcatg
     1321 gaaggtgagg cgctcgcaga tttcgcggac gggcacacat gagcctgacg ctcgttggcg
     1381 tcgtcgccgt cgctgataat ggcgtcattg gtcagggcaa tgcgctgcct tggcacattt
     1441 cagccgacct caagcgcttt cgcgcgctga cgcttggcaa acctttgatc atgggccgca
     1501 agacgtttgc ggcgatcggc cgtgcgctgc cgggccgcga aacgatggtc gtgacgcgcg
     1561 acgcgaaatt tgcgccgcca gccggcgtgt tcacggcgac cacaatcgat gccgccttgc
     1621 agctggcgac gacacgtgcc gatgttttgg gtgcgtcgga gattattgtt gctggcggcg
     1681 gcgaaatcta cgcggcgttg ctcgaccgga tagacctttt gcacatgact tacgttcatt
     1741 gcacgcccga gggggacgcc ttctttccgc ccattgagtg gtcacactgg cgtgaagtct
     1801 ttcgcgaaga ccacttatcc gaaactggtg gtgatgcagg atatacgtgg gccgactata
     1861 tccgtaccgg ctctctttcg ctggcgccga aagccctcta agacaagcgc caccgaggaa
     1921 ggatttcatg ccctggagta atgacggtaa ggacggcggc tcttggaagc cggaaaatcc
     1981 cggcccgtgg ggccaaccgc aggcgccgaa tcccgtcaat ttcgagggat gggtgcgcct
     2041 ggcacaggcg cggctgcagg gctggctgcc cggcggcggc ctcggcggcc gcgcgcttgc
     2101 ggtgcttggc cttctcggcg tcctcctgtg gctgctgaca gggtgctata caatcggccc
     2161 gaatgaggtc ggcctcaaca tgatcttcgg ccgctatacc ggcaagacga cctcgggtct
     2221 aaattacaac ctgccgtatc cgattggctc cgtccaaaag ctcgcggtga cggatcgcaa
     2281 cacaaccgat atcggcttca tttcgcggat ggacgatcgc accggcgagc aggcgacctt
     2341 cgatttgcct gaagagagcc tgatgctgac cgacgatcag aacatcgcgg acgtgaaatt
     2401 tgtcgtgatc tggcagatcg atccgtcaca tcctgaagat tacgccttca acgtcgccga
     2461 tcccgacgac acggtgaaag ccgtggcgga gagtgcgatg cgcgc
//
LOCUS       DASWOG010000708         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_256213,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000708 DASWOG010000000
VERSION     DASWOG010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256213"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGB93_14490"
     CDS             complement(<1..732)
                     /locus_tag="VGB93_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0147325.1"
                     /translation="MPFTVATWNINSVRLRIDLVGKFLSDHAPDVLCLQETKCRDAEF
                     PAAAFRKFGYEHIALNGQKGYHGVAIVSKTPFGIVDRRPFCDEGDARHISVTVAPTNL
                     RAFTLHNFYVPAGGDEPDADINPKFAHKLAFLDEMASWIRADRVAGGDAIMVGDLNVA
                     PLETDVWNHKALLKVVSHTPVEVEKLGAVFAAGPWVDALRRYVPAEEKLYTWWSYRSP
                     DWAKANKGRRLDHIWTSAALAPNLCG"
     gene            complement(1025..>1886)
                     /locus_tag="VGB93_14495"
     CDS             complement(1025..>1886)
                     /locus_tag="VGB93_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0147326.1"
                     /translation="GPNQPARKAKLMSMTNASGLKVPCDLAVAIDRQRCSFRIGPMRL
                     NFVRAGVSGLFLGSAMAFATFATMTFAEAATAAATPPPVSQNSAPPPAATPPFKPMDP
                     HVAIQKADDYFNSTRSMIGDFVQISGDRRSEGKVYIQKPGKMRFEYAEPATLDIVADG
                     MTVAVIDRKLATQDFYFVWQTPLKFLLKDRIDLLRDLAVTDVISDPDNVTITVVDQET
                     FGGTSRIKLMFDPATFQLKQWEVTDPQGNETLVSLFNVDRSTPPDPSVFQIKSSRLDS
                     SNSRLLNRSN"
BASE COUNT          387 a          523 c          596 g          380 t
ORIGIN      
        1 gccgcatagg ttcggggcca aagccgcgct tgtccagatg tggtcgagtc ggcggccttt
       61 attcgccttg gcccaatcgg gcgagcgata gctccaccac gtataaagtt tttcctcggc
      121 ggggacgtag cggcgcagcg cgtcgaccca gggtcctgcg gcgaaaaccg cgcccagctt
      181 ttcaacctcg accggcgtat ggctcacgac tttgagcaac gccttatgat tccagacatc
      241 ggtttcgagc ggcgcgacat tgaggtcgcc gaccatgatg gcatcaccgc cggcgacgcg
      301 gtccgcgcgg atccagctcg ccatctcgtc gaggaaggcg agcttgtggg cgaatttcgg
      361 attgatgtcg gcgtcgggct cgtcgccgcc ggccggcacg tagaaattat gcagcgtaaa
      421 ggcccggagg tttgtcggcg caacggtcac gctgatatgg cgcgcatcgc cctcatcgca
      481 aaacggccgc cgatcgacga tgccgaacgg tgttttcgag acgatggcga cgccgtggta
      541 gcccttttgg ccgtttagcg cgatgtgctc gtagccgaac ttgcggaacg cggcggcggg
      601 aaattcggca tcccggcact tcgtttcctg caggcaaaga acgtccggtg catggtccga
      661 caggaatttg ccgacgagat cgatacgcaa acggacggaa ttgatattcc aggtggcgac
      721 ggtaaacggc aattttgatc cgggaaatct gaagctatcg ttgttgagca cgcggtacgc
      781 tgggcggcaa ggaaagcctc gccgcaatca caggattttg ccgcgggtgc gtgcggcgca
      841 taaaaatagg gcgcccgatc gagatcggac gccccgtgga gcgacactat accgggaggg
      901 ggtccggcgt agccggcgag acagaacggg gggaattcgt caagccaacc gtatcactct
      961 ccgaaaagtg agataggtct gcgcaaatcg gactgcaagt ccggccgcaa attattgcga
     1021 aacgttaatt gctccgattc aggagccgac tattcgagct gtcgagccgg ctggacttga
     1081 tctggaaaac ggaagggtcc ggcggcgttg aacgatcgac gttgaagagc gaaaccagcg
     1141 tttcgttacc ttgcggatcg gtgacttccc attgttttag ctggaaagtc gctgggtcga
     1201 acatcagctt gatgcgcgat gtgccaccaa aggtttcctg atcgacgacc gtgatcgtca
     1261 cgttgtccgg atcactgatg acatcggtca cggcgaggtc gcgcaaaaga tcgatccgat
     1321 ccttgagcag gaatttcaac ggtgtctgcc agacgaaata gaaatcctgc gtggcgagct
     1381 tgcgatcgat caccgcgacc gtcatgccgt cggcgacgat gtcgagcgtt gcgggctcgg
     1441 catattcaaa gcgcattttg ccgggcttct ggatgtagac tttgccttcc gagcgccggt
     1501 ctccgctgat ctgcacgaaa tcgccgatca tcgaccgcgt cgaattgaaa taatcgtcgg
     1561 ccttctgaat cgcgacatgc ggatccatgg gcttaaaggg cggggtcgcg gccggcggcg
     1621 gcgcgctgtt ttgcgagact ggtggcggcg tcgccgctgc tgtggcggct tcggcgaagg
     1681 tcatcgtggc gaaggttgca aaggccattg cgctgccaag aaagagacct gaaacgcccg
     1741 cgcgaacgaa attcagtcgc atcgggccga tccgaaaact gcatcgctgg cgatctatcg
     1801 caacagcgag atcgcaaggc acttttaggc ccgatgcatt ggtcatcgac atcaatttcg
     1861 cctttcgcgc cggctgattc ggtccc
//
LOCUS       DASWOG010000709         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_218830,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000709 DASWOG010000000
VERSION     DASWOG010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218830"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VGB93_14500"
     CDS             <1..900
                     /locus_tag="VGB93_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0147327.1"
                     /translation="SYISIPNNSWFPYNDCQNPIPGITPSPSSVIGFPTASSTFNCLT
                     NGEASLALKQAQGTTLTSMPGFTLSYNSLDNNRSPTDGIHAEWREDFAGAGGSERYVR
                     STADLRYYHDIYDEIVGVLHFQGGILNAVGSNGTKIVDNFNMGPELVRGFQPDGLGPR
                     DVSSGVDPEGNPLGGTKLWGASAEADFPIPYLPREVGLRGAVFTDFGALWGYNGPTVF
                     PQFASSPIVSKGAPAGCGAGCSFQGDAITVGGDSTDIRASVGVSLIWSSPMGPIRFDF
                     AKAVLKNQFDQTQIFNFTGGTTF"
     gene            985..>1610
                     /locus_tag="VGB93_14505"
     CDS             985..>1610
                     /locus_tag="VGB93_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HEY0147328.1"
                     /translation="MNDPVFFRPAIRPSLAEVVAWTGAVPAAAVDLAALFVENVAPLD
                     EASPGSLTFCDNSKYLDDLALTHALACFVPPRFAARVPPVTLALLTPVPARDLTRVLA
                     QLYTDALTPGTMFDSKGESPGADIHPEARLEPDVVVDPGVVIGPHAEIGSGTMIGANA
                     VIGPNVRIGRGCAIGPGAVIANALIGNRVIIHAGVKIGQDGFGFIAGP"
BASE COUNT          270 a          511 c          475 g          354 t
ORIGIN      
        1 tcgtatatct ccatcccgaa caattcctgg ttcccgtaca acgactgtca aaacccgata
       61 cccggcatta cgccgagccc gtcgtcggtc attggctttc cgacagcctc ttcgactttc
      121 aactgcttga cgaacggcga agcctcgctg gctttgaaac aggcccaggg cacgacgctc
      181 acttcgatgc ccggcttcac gctgtcgtac aattcgctcg ataacaatcg cagtccgacg
      241 gatggcattc atgccgaatg gcgtgaagac tttgccggcg ccggcggcag tgagcgctac
      301 gtccgttcga ccgcggatct gcgttattat cacgacatct atgacgagat cgtcggcgtg
      361 ttgcacttcc agggcggtat tcttaacgcc gtcggttcga acggcaccaa gatcgtcgac
      421 aacttcaaca tgggccccga actggtccgc ggcttccaac cggatggtct cggtccgcgc
      481 gacgtgtcgt cgggcgtaga tccggaaggc aaccccttgg gcggcaccaa gttatggggc
      541 gcttcggctg aagctgactt cccgatcccg tatctgccgc gagaggttgg cctgcgtggc
      601 gccgtattca ccgatttcgg tgcgctctgg ggttataatg ggccgaccgt cttcccgcag
      661 tttgcttcca gcccgatcgt cagcaagggg gcgccggccg gttgcggagc tggctgttcg
      721 ttccagggtg acgcgatcac agtcggcggc gattcgactg atatccgtgc ctcagtgggt
      781 gtctcgctca tctggtcttc gccgatggga ccgattcgct tcgatttcgc caaggctgtg
      841 ctgaagaatc aattcgatca gacgcagatc ttcaacttca ccggcggcac cacgttctga
      901 tcgccgccgg gacgtcggcg tcgaaatcgt ctaaggtgtc acgtctggcc ccgcctttgg
      961 tggggccttc ccggctttcg ccttatgaac gatccagttt tctttcgccc tgcgattcgc
     1021 ccaagccttg ccgaggtcgt cgcctggact ggcgccgtgc cggcggctgc agtcgatctc
     1081 gcggcgcttt tcgtcgaaaa tgttgcgccg ctcgacgagg ccagccctgg gtcgctcacc
     1141 ttttgcgaca attccaaata tctcgatgat ctggccctta cacatgcttt ggcgtgtttt
     1201 gtaccaccgc gtttcgccgc gcgcgtacca cctgtgactc tggcattgtt gacgccggtg
     1261 ccggcgcgcg acctgacccg cgtactcgca caactttaca ccgacgcgct cacacccggc
     1321 actatgttcg attcgaaagg tgaaagcccc ggcgcggata tccatcccga ggcgcggctt
     1381 gaaccggacg tcgttgtcga tccgggcgtc gtcatcggac cgcatgccga gattggctcg
     1441 ggcacaatga tcggcgccaa tgcagtcatc ggcccgaatg tgcggatcgg gcgcggctgc
     1501 gccatcgggc ccggcgcggt gatcgccaac gcgctgatcg gcaacagggt catcatccac
     1561 gccggggtca agatcggtca ggacgggttc ggctttatcg ccgggccgaa
//
LOCUS       DASWOG010000710         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_365254,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000710 DASWOG010000000
VERSION     DASWOG010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365254"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="ntrC"
                     /locus_tag="VGB93_14510"
     CDS             complement(<1..1092)
                     /gene="ntrC"
                     /locus_tag="VGB93_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY0147329.1"
                     /translation="MGGNILVADDDAAIRTVLSQALTRVGYEVRTTGNVATLWRWVQA
                     GEGDLIITDVVMPDDNAFEILPRIKKLRPDLPIIVMSAQNTFMTAIKASERGAYDYLP
                     KPFDLRELVNIVGRAMSEPKTRTRPEPQVDLEGMPLVGRSPAMQEIYRVLARLMQTDL
                     TVMITGESGTGKELVARALHDYGKRKNGPFVAINMAAIPRDLIESELFGHEKGAFTGA
                     NQRSAGRFEQSEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTNVRIVA
                     ATNKDLRNLIHQGLFREDLFFRLNVVPLRLPPLRERAEDVPDLVYHFFALAASEGLPL
                     KHVEQPALERLKRHRWPGNIRELENLVRRL"
     gene            complement(1134..>2249)
                     /locus_tag="VGB93_14515"
     CDS             complement(1134..>2249)
                     /locus_tag="VGB93_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0147330.1"
                     /translation="QKASEAFRDAVKLLNALPHPVLAVAPDGFVVEANVATESFFGIS
                     RSILIQQTLANLLPYDSVLLALVGQVTATHAAITEYRVDLGIPRLGTEKSVDLFVMPV
                     SDFDEGVVVMIQERSMAEKMDRQLTHRGAARSVSGMAAMLGHEIKNPLSGIRGAAQLL
                     EIGANDSDRALTRLICTETDRIVKLVDRMEAFSDSRPLERESVNIHTVFDHVKRIAKA
                     GFARHIRFTESYDPSLPPVLGNRDQLIQIFLNLVKNAAEAIGESSEDGEIELSTAFRP
                     GVRLKLPNSDRSVNLPLQFCVRDNGPGIPQEIRQHLFEPFITTKSSGSGLGLALVAKL
                     IGDHGGTIECESQPRRTIFKVLMPMYAPTDGRSAKKT"
BASE COUNT          466 a          674 c          648 g          461 t
ORIGIN      
        1 gagacggcgc acgagatttt cgagttcgcg gatattgccc ggccaacggt gccgcttaag
       61 ccgctcaagc gccggttgct cgacatgttt cagcggcagc ccctcggaag ccgccagcgc
      121 aaagaaatga taaaccagat ctggaacgtc ttcggcgcgc tcacgcagcg gcggcaagcg
      181 aagcggcacg acattgagcc ggaagaacag gtcctcacgg aacagtccct gatggatcag
      241 attacgcagg tctttgttcg tcgcggcaac gatccgcaca ttggtcttga tcggcgtacg
      301 gccgccgacg gtcgtatatt cgccctgctg caggacgcgc agaagccgtg tttgggcctc
      361 catcggcata tcgccaattt cgtcgagaaa gagcgtgccg ccctcggact gctcgaaccg
      421 tccggccgag cgctgattgg cgcccgtaaa ggcacccttt tcgtggccga aaagttcgct
      481 ctcaatcagg tcgcggggga tcgccgccat gttgatggcg acgaaggggc cgttcttgcg
      541 cttgccgtaa tcgtgcaatg cgcgcgcgac gagctctttg ccggtgccgg actcgccggt
      601 gatcatcacc gtcagatcgg tctgcatcag gcgggcgaga acgcggtaaa tctcctgcat
      661 cgccggtgaa cgcccgacaa gcggcatgcc ttcgagatcg acctgcggct ccggccgagt
      721 tcgtgtcttc ggctcgctca tcgcgcgccc gacgatattt acaagttcgc gcagatcgaa
      781 cggcttcggc aaataatcat aggcgccgcg ttcggacgcc ttgatcgccg tcataaaagt
      841 gttttgcgcg ctcatcacaa tgatcggcag atcggggcgc agctttttga tgcgcggcaa
      901 aatctcgaac gcattgtcgt caggcatgac gacgtccgtg atgatgagat cgccctcacc
      961 ggcttgcacc caacgccata gagtcgcgac attgcccgtc gtgcggacct catagccgac
     1021 acgagtcaac gcctggctca acaccgtccg gatcgcggca tcgtcatcgg cgacgagaat
     1081 attcccgccc attgcagttt tgctcctgag ggctcccgag agccaatcct tttctaagtc
     1141 tttttggcgc tgcggccgtc cgtcggtgcg tacatcggca tgagcacttt gaatatcgtc
     1201 cgacgcggct gcgactcgca ttcgatggtt ccaccgtgat caccaatgag tttggcaacc
     1261 agtgcaagtc cgaggccact ccccgagctc ttcgtcgtga taaaaggttc aaacagatgc
     1321 tggcgaattt cctgtggaat gcccggcccg ttgtcgcgaa cgcaaaattg gagaggcaaa
     1381 ttcaccgatc gatccgagtt tggcaacttt agccgaacgc ccggacgaaa ggccgtcgat
     1441 agctcgatct cgccgtcctc gcttgattca ccgattgcct cagcagcgtt cttaacaaga
     1501 ttgaggaaaa tctgaatcag ttgatcgcga ttgccgagca ccggcggcag cgatggatcg
     1561 tagctttcgg tgaagcggat atgccgcgcg aaaccggcct tggcgatccg ctttacatga
     1621 tcgaaaacgg tgtgaatgtt gacgctttcg cgctccagcg gccggctgtc tgaaaacgct
     1681 tccattcggt cgacgagctt cacgatccga tcggtttctg tgcagatcag ccgcgtcaac
     1741 gcgcggtcgg aatcattggc gccgatctcg agcaattgtg cggcgccgcg aatgcccgac
     1801 aacgggttct tgatctcatg cccgagcatc gccgccatac cggagaccga ccgcgccgca
     1861 ccacgatgcg taagctgacg atccatcttt tcggccatcg accgttcctg gatcatcacc
     1921 acgacaccct cgtcgaagtc cgacacgggc atgacgaaga gatcaaccga tttctccgtt
     1981 ccaagccgcg ggatacccag atcgacgcga tattctgtaa tcgcggcatg tgtcgcggtg
     2041 acctggccga caagcgcgag caggacggaa tcgtaaggca gcagattggc cagtgtttgc
     2101 tgaatgagaa tcgatcggct gatgccgaag aaagattccg tcgcaacatt ggcttcgacg
     2161 acgaagccgt caggcgcgac cgcaagcacc ggatgcggca gcgcattcaa aagttttacg
     2221 gcatcgcgaa aagcttcgct ggccttttg
//
LOCUS       DASWOG010000711         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_347300,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000711 DASWOG010000000
VERSION     DASWOG010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347300"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VGB93_14520"
     CDS             complement(<1..1285)
                     /locus_tag="VGB93_14520"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEY0147331.1"
                     /translation="MDYDVIIVGYGPTGKILARRLSDAGHSVAIVERWPETYPLPRAL
                     VYDHEVKRIFHALGLAAQVDAISRPMSHYVWYNADWKILADIDESRESISGGRHAYLA
                     NQPDLERVLARDLLGRRGVALFLGKEAQSVEQTADIARVIIAPFDAQTQEADLSKTER
                     LSAKYVVGCDGANSLVRETLGSTFLDHGFDAQFLVVDVRPHDPSALQFPDAIQYCNPE
                     RPMTIVWGGVVNRRWEFMIKPGEDATEFAKSPKTWELLLRWMKPGEGELVRHAVYRFR
                     SMLARGWRNGRLLIAGDAAHLMPPFLGQGMCSGIRDSWNLAWKLNRVLKGSSRDGLLD
                     AYEAERGPNVDAYVRLSMEVGKIVCAPDAEAANARDAAFASGALPPPLDFPGLSGGII
                     AEGDGAGLLTPHDEFEMPLGFRRLDDVIGRNFALIG"
     gene            complement(1524..1598)
                     /locus_tag="VGB93_14525"
     tRNA            complement(1524..1598)
                     /locus_tag="VGB93_14525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1564..1566),aa:Gly,seq:gcc)
     gene            1779..2675
                     /gene="trxA"
                     /locus_tag="VGB93_14530"
     CDS             1779..2675
                     /gene="trxA"
                     /locus_tag="VGB93_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0147332.1"
                     /translation="MAELLGQTEPPPGATIATTTAAFATDVLAESGRRPVLVDFWAPW
                     CEPCKQLTPILEKAVQAAQGKVKLVTMNIDEHPQIAGQLGIRSIPAVIAFQRGQPVDG
                     FMGALPESQVRGFIERLAGPLPEEEDLHAMADAALAAGEPLAALEIFRELYEKDSGDL
                     AALAGLIKTNVATGELEDAKKLLAQVPETAAHDPAVAAARAAVENATQAAGLGEIGEL
                     ERRVAAEPNDPQARFDLAIALNAQSRRGEAADALLAIIKRDRDWNDAAARKQLLQFFE
                     AWGNMDAATVAARRKLSTLLFS"
BASE COUNT          521 a          848 c          848 g          462 t
ORIGIN      
        1 cgccgatcaa cgcgaaattt cggccgatga catcgtcaag ccggcgaaaa cccagaggca
       61 tttcaaattc atcgtgtggg gtgagcaagc ccgcgccgtc accctcggcg atgatgccgc
      121 cggagagtcc cggaaagtcc agcggcggcg gcagtgcgcc gcttgcgaag gccgcgtcgc
      181 gcgcgttcgc ggcttcagcg tccggtgcgc agacgatctt ccccacttcc atcgacagcc
      241 gaacgtacgc atccacgttg ggcccgcgct cggcctcgta ggcgtcgagc agaccgtcgc
      301 gcgaagagcc tttcaatacg cgattgagct tccaggcgag gttccaggag tcgcgaatgc
      361 ccgaacacat tccctgcccc agaaagggcg gcatcagatg cgctgcgtcg ccggcgatga
      421 gaagccgccc gttgcgccag cctcgggcga gcattgagcg gaatcgataa acggcatgac
      481 gcacgagttc gccctcgccc ggtttcatcc agcgcaagag cagttcccat gtcttgggag
      541 acttggcaaa ttcagtcgcg tcttcgcccg gcttgatcat aaactcccag cggcgattga
      601 cgacgccgcc ccaaacgatc gtcatcggcc gctcgggatt gcaatattga atggcgtcgg
      661 ggaattgcag ggcggacgga tcgtgcgggc gaacgtcgac gaccagaaat tgcgcgtcga
      721 agccatggtc gagaaacgtc gatccgagag tctcgcgcac gaggctgttg gcgccatcgc
      781 agccgacgac atatttcgcg gacagccgct cggtcttcga gagatcggcc tcctgcgtct
      841 gagcgtcgaa cggcgcgatg atgacgcgcg cgatgtcagc cgtctgctcg accgattgag
      901 cctctttgcc caggaacaac gcgaccccgc gacggcctag gaggtcgcgc gcgagcactc
      961 gttcgagatc tggctgattg gcgagatagg cgtggcgtcc gccggagatg gattcgcggg
     1021 attcgtcgat gtcagccaga atcttccagt cggcgttgta ccagacgtaa tggctcatcg
     1081 gccgcgaaat cgcatcgacc tgcgcggcca atccgagcgc atggaatatg cgcttgacct
     1141 cgtgatcata gacgagcgcg cgaggcagcg gataagtctc gggccatcgc tccacgattg
     1201 cgacgctgtg ccccgcatcg ctgagccgcc gggcgagaat cttacctgtc ggaccgtagc
     1261 ccacgatgat gacatcgtaa tccatcggcg ccctcccgtt tgactatgca acgggcttcc
     1321 gtgcccgtga agccctagcc aaaaaagcgc ggcgcaatcg cgcgagcccg ctgtccggac
     1381 gagatctaaa gcgacgtgat cctgcggctg tcaaagggag ataagggcgc tcgggaacat
     1441 tgtccgggcg cctattgcct accttgaagc ggcgtttgga ccccgaaggg ccgtcttatt
     1501 gctctgattt tacgaagtat gtttggagcg ggcgaaggga ttcgaaccct cgaccccaac
     1561 cttggcaagg ttgtgctcta cccctgagct acacccgcat ccatgccggg caagcccagc
     1621 gtcccggcta tatgccgcaa accttcgacc attgcaatca agcccgcgtc ataaaatttt
     1681 cgccgccgcg cgagacgcct taaccgcccg gttgcgaaag gcttgaccgc agctcatcta
     1741 tgcgagaagc gggaccggcg aacacaggag agcggcagat ggcggagttg ttgggacaga
     1801 cggagccgcc gccgggcgcg actatcgcga ccacgacggc tgccttcgcg accgacgttc
     1861 tggccgaatc cggccgccgc ccggtcctcg tcgatttctg ggcgccctgg tgcgaaccct
     1921 gcaaacaact cacccccatt ctcgaaaagg cggtgcaggc ggcgcagggc aaagtgaagc
     1981 tcgtaacgat gaatatcgac gagcatccgc agatcgccgg ccagcttggc atccgctcga
     2041 tcccggccgt cattgcgttt cagcgcggcc agccggtcga cggtttcatg ggcgcattgc
     2101 cggagagcca ggttcgcggc ttcatcgagc gccttgccgg gccattgccc gaggaagaag
     2161 accttcatgc gatggcggac gcggctctcg cagccggcga accgctggcc gcgctggaaa
     2221 ttttccgtga gctttatgag aaggattctg gcgatctcgc cgcgctggcc ggcctgatca
     2281 agaccaatgt cgcgaccggc gaactcgaag atgccaaaaa gctcctggcg caagttcccg
     2341 aaaccgcggc gcacgatccc gcggtcgcgg cggcgcgggc cgcggtcgag aacgccaccc
     2401 aagccgcggg gctgggtgaa atcggcgaac ttgagcggcg cgtggcggct gaacccaacg
     2461 acccgcaggc gcgtttcgac cttgcgattg cactcaacgc gcaaagccgg cgcggcgagg
     2521 cggcggacgc gctgctcgcg atcatcaaac gcgaccgcga ttggaatgac gcggccgcgc
     2581 ggaagcaatt gctgcaattc ttcgaggctt ggggcaacat ggatgcggcg accgtcgccg
     2641 cgcggcggaa gttgtcgacg ctcttatttt cctgagccg
//
LOCUS       DASWOG010000712         8465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_347306,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000712 DASWOG010000000
VERSION     DASWOG010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347306"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VGB93_14535"
     CDS             <1..810
                     /locus_tag="VGB93_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY0147333.1"
                     /translation="LFGFALMALGQRGKLLTDVLDQATLAVFGIINIVMRAAPVGAFG
                     AMAYTVGKYGTQVLGALAGEIATFYLTSALFVIVVLGTIARLAGFNIFKFLVYLKDEL
                     LIVLGTSSSESGLPGLIEKLEALGCSRSVVGLVVPTGYSFNLDGTNIYMTLATLFIAH
                     ALGVDLSFGQQMTILLVAMLTSKGATGVTGAGFITLAATLSSVSPALVPGMAILLGID
                     KFMSECRALTNFIGNGVATVVISASEGELDRGKLRAALDAGPMMEEMQGIG"
     gene            1036..2133
                     /locus_tag="VGB93_14540"
     CDS             1036..2133
                     /locus_tag="VGB93_14540"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HEY0147334.1"
                     /translation="MQMRTALALTFLLALAPAAEACTTLLLKAKDGAVVSGRTMEFAI
                     DVKSKVVVIPAGTQMKGSLPDGAIGISYTTKYGMMGANALGFPVIVDGINDQGLFVSD
                     LYFPGYAGYTKATPQNASRAMASYEFGNWLLGNFATVDEVRAHVQNIVLVSTPVDVLG
                     GPPPLHFIIRDRSGKSLVIEPINGKLMVYDDPLGVMTNSPGFDWHMTNLRNYATLTDQ
                     NVAPLHLSNNVTLRPFGQGSGMFGLPGDSTPPARFVRAVAFSQTALQPATAAEAVLQV
                     FHIMNNFDIPLGLVRDKSGNALHVDYTVWTSVADLQNDRWYFRTYNDQAIRMIDLHAA
                     LALAGKKVGFIPMDSQQTVIDASTALRAAND"
     gene            complement(2229..2600)
                     /locus_tag="VGB93_14545"
     CDS             complement(2229..2600)
                     /locus_tag="VGB93_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0147335.1"
                     /translation="MSQKPLDFSLLLKSKLPRRSFVADDVIFREGDKAEALFVIEKGQ
                     VEIQGGRRVVAILGPGEFFGEMSLLDPAPRSGTAVAVTDVTLVPVSEKEFLRIVGETP
                     SFALDLMRLFVRRIRARDGYY"
     gene            2834..3334
                     /gene="ssb"
                     /locus_tag="VGB93_14550"
     CDS             2834..3334
                     /gene="ssb"
                     /locus_tag="VGB93_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0147336.1"
                     /translation="MAGSVNKVILIGNLGRDPEVRRLNSGDSVVNFSVATTESWRDKA
                     TGERKDRTEWHNVVIFNENLGKIAEQYCKKGSKIYLEGQLQTREYTDKDGNQRRTTEV
                     VLQRFRGELTLLDSRGGARGESFDDRGGAGEASSSGGSFGRSSPMDSDKRPSRAAEMI
                     DDDIPF"
     gene            3371..4477
                     /locus_tag="VGB93_14555"
     CDS             3371..4477
                     /locus_tag="VGB93_14555"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0147337.1"
                     /translation="MIVEKKVFELPEFRTSGGQLIKNIRIGWESYGTLNEARSNAILI
                     AHYFSGTSHAAGRYAKEDVFPGYWDALIGPGKAIDTDRYFVFSSDTLVNLNARDPHVV
                     TTGPASLNPDTGASYGLDFPLVTIRDFAAVQKALVDHLGICKLHAVMGPSMGGLQTFA
                     WAATYPDMMDRIVPVICAGEVDAWLIAWLGLWGAPIMADPAWKNGAYPLDEQPLAGLK
                     LALELVTLQANHWIWADTQFGRAFAEPGADPGLALGNRFKVEAAVSGIAAERAKISDA
                     NHLLYLVKANQTFAAGPTGTLADLEKIKAKTLLLYSPEDQVFRPDKSRATAAAIAKGG
                     ATVETGEIHGPFGHANGLFAMAPLGDRIKEFLTV"
     gene            4711..7452
                     /gene="gyrA"
                     /locus_tag="VGB93_14560"
     CDS             4711..7452
                     /gene="gyrA"
                     /locus_tag="VGB93_14560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0147338.1"
                     /translation="MADNNENGAGPSDSDVRPVSISDEMRRSYLDYAMSVIVSRALPD
                     VRDGLKPVHRRILFSMYENNYLPDRAYNKSARVTGDTMGKYHPHGNLAIYDALVRMAQ
                     PFSMQLPLIDGQGNFGSVDGDPPAAERYTEVRLATSALPLLDDLDNETVDFQANYDGR
                     EREPVVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVTDAVFALIDRPEITVEELIG
                     IIPGPDFPTGGSIIGRGGIRAAYLNGRGSILVRAKVEVEEIRKEREALIVTEIPYQVN
                     KATLVEKIAELVREKKVEGISDLRDESDRQGMRIVIELKRDAVSDVVLNQLWRFTSLQ
                     SSFPANMIALNGGRPETLNLKDFLRAFVDFREEVVTRRTKFLLGKARDAAHLQVGLAI
                     AVANIDEVIRVIRTSPDATTAREALMSREWPARDMAPLIALIADPRHVLTADGMCRLS
                     EAQARGILELRLQRLTALGRDEIGQALEKHAAEIADYLDILRSRARLFGIIKDELQAV
                     KLAHATPRRSIILDSDADFEDEDFIQREDVVVVVSHAGYIKRVPLSTYRAQRRGGKGR
                     TGMQTRDEDFVAKLFVASTHTPVLFFSSLGQAYKEKVWRLPASAPQARGKALVNILPL
                     EQGERITTIMPLPEDEEQWEKLDVIFATTRGTVRRNKLSDFAQVNRAGKIAMKLDPGE
                     EIVNVQICNESDDVLLTSADGQCIRFPVTDVRVFKGRDSMGVRGIALGEGDRVISLAI
                     LHHIEADSGERTAYLKMRRAIAGEAEATEPANDDANGEEAAAAALSSERYAQMSAAEE
                     IILTISANGYGKRTSSYEYRITGRGGKGIVAMAVNQRNGQLVASMPVEETDGIMLVTN
                     GGQMIRCPVEGIRVAGRSTQGVIVFNTAEDERVVSVECISEDEAAENGEASDEAASE"
     gene            7514..8017
                     /gene="coaD"
                     /locus_tag="VGB93_14565"
     CDS             7514..8017
                     /gene="coaD"
                     /locus_tag="VGB93_14565"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0147339.1"
                     /translation="MTIKALYTGTFDPLTNGHVDIIARAARFCDELVVAIGVHPGKTP
                     MFAAAERTDFIRAACGGLTANHSCKLSVLTFSGLAVTAARETGATLLVRGLRDGTDLD
                     YEAQMTGMNAIMAPEIETIFLSASPQVRHIAATLVRQIALLGGDIAPFVPDVVAKALA
                     VKLNNAP"
     gene            8078..>8465
                     /locus_tag="VGB93_14570"
     CDS             8078..>8465
                     /locus_tag="VGB93_14570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0147340.1"
                     /translation="MFLTAPLAAADLKNEIYLYTKDGRVTLLLRPDLAPKTVEQIKIL
                     TKRGFFNGLKFHRVIEGFMAQTGDPTGTGTGGSDLPNVPAEFNKAHFKRGSLGMARAD
                     DPNSGNSQFFICFGDAGFLDGQYTNYG"
BASE COUNT         1671 a         2639 c         2476 g         1679 t
ORIGIN      
        1 ctgtttggct ttgccctgat ggcgcttggc cagcgcggca agcttctgac cgacgtgctc
       61 gaccaggcaa cgcttgcggt tttcggaatc atcaacatcg tgatgcgggc cgcgcccgtc
      121 ggtgccttcg gcgcgatggc ctatacggtc ggcaaatacg ggacgcaggt gctcggcgcg
      181 ctcgccggcg agatcgcgac gttttacctg acgtcggcgc tttttgtcat cgtcgtgctc
      241 ggcacgatcg cccgtctcgc gggcttcaat attttcaagt ttctcgtcta tctgaaggac
      301 gaattgctga tcgtgctcgg cacaagttca tcggaaagcg gccttccggg tctcattgaa
      361 aaactcgagg cgctgggctg ttcgcgctcg gtcgtcggcc ttgtcgtccc gaccggctat
      421 tcgttcaatc tcgacggcac gaacatctat atgactctgg cgacgctctt catcgcccat
      481 gcgctcggcg tcgatctttc gttcggccag caaatgacga tcctgcttgt cgccatgctg
      541 acctcgaaag gcgcgacggg tgtcaccggc gccggcttca tcacgctcgc ggcgacgctc
      601 tcctccgtct cgcctgccct ggtccccggc atggccattt tgctcggcat cgacaaattc
      661 atgagcgaat gccgggcact gaccaatttc atcggcaatg gcgtcgcgac ggtggtgatc
      721 tccgcgtccg aaggagagtt ggatcgcggg aaactccgtg cagcgctcga tgcgggaccg
      781 atgatggaag agatgcaagg aatcggctga ttacaccggt ctttgcgctg atttgaatga
      841 cttgcttcga taatcggaaa gcatatcggc gaataagccc gccgccgagc ggatcttaag
      901 atgaatcatt acggcaaaca gccgcctacc gcctaagcct gtccccgaag tcctacgcgc
      961 cgacaggtga ccgcatattc ccgcgatttc cgctcacgcc tgggaatctg accagcctca
     1021 ttgaggagaa cagccatgca aatgcgcacc gctctcgctc tcacgtttct tcttgccctc
     1081 gccccggcgg ctgaggcctg cacaacgctc ttgctcaagg ccaaggatgg agccgttgtg
     1141 agtggccgga caatggaatt cgcaatcgat gtaaagtcga aagtcgtcgt cattcccgct
     1201 gggacgcaaa tgaagggctc gttgccagac ggcgctatcg gtatcagcta cacgacgaaa
     1261 tacggcatga tgggtgccaa tgccctcggc tttccggtga tcgtcgatgg catcaacgat
     1321 caggggctct ttgtcagcga tctctatttc ccgggatatg ccggttacac caaggcgacg
     1381 ccgcaaaatg catcgcgcgc aatggcctcc tacgaattcg gcaattggct gcttggcaat
     1441 ttcgcgacgg tcgacgaggt acgcgcgcac gttcaaaata tcgtgcttgt ctcgactccc
     1501 gtcgatgtgc tgggcggacc gcccccgctt cacttcatca tccgtgatcg ctccggcaaa
     1561 tcgctggtaa tcgagccgat caacggcaag ttgatggtct atgatgaccc gctgggtgtg
     1621 atgaccaatt cgcccggctt tgactggcac atgaccaatc tgcgcaacta cgccacgctg
     1681 acggaccaaa acgtcgcgcc gctgcatctc tccaacaacg tcacgctgcg accgttcggc
     1741 cagggaagcg gcatgtttgg cctgccgggc gacagcacgc cgccggccag gtttgtccgc
     1801 gcggtcgcct tttcgcagac tgctttgcag ccagccaccg ccgccgaagc cgtactgcag
     1861 gtcttccaca tcatgaacaa tttcgacatt ccgctggggc tggtccgcga caagagcggc
     1921 aacgcgctcc atgtggatta tacggtctgg acgagcgttg ccgatctgca gaatgatcga
     1981 tggtacttcc gtacatacaa cgaccaggcg atccgcatga tcgacctcca cgccgccttg
     2041 gcgcttgcgg gcaagaaggt cgggttcatc ccgatggatt cgcagcagac tgtcatcgat
     2101 gcgtcgacgg cgctccgcgc cgccaacgat taacggaacc ggacgctgag gcggcgcagg
     2161 ccacagcccc gcgccggtcg ttctgcccca taagcccgcg gcgatccctg cgcccggggc
     2221 ctgccctgtc aataataccc gtcccgcgcc cggatgcgtc gcacgaagag gcgcataaga
     2281 tcgagcgcga agcttggcgt ctcgccgacg atccgcaaaa actccttctc ggagacgggc
     2341 acgagcgtaa catccgtcac ggccacagcc gtgccgctgc gcggcgccgg atcgaggagc
     2401 gacatttcgc cgaaaaattc gcccggcccc aggatggcga cgacgcgccg gccaccctgg
     2461 atttccacct gccctttttc gatgacgaag agcgcttcag ccttgtcgcc ctcgcggaag
     2521 atcacgtcgt ccgcgacgaa cgagcgtcgg ggcaacttgg atttcaacaa gagactgaag
     2581 tcgagcggct tttgcgacat gattgcttcc tggccttgct gtggcctcgt ccggcgctga
     2641 gcctaagccg cgcagcgcgc gtttgccaag atttgcgaca ccacgggcgt caactttcta
     2701 gcttgccatg ttgctgaaga acccttaaga acaaataaag aacaatgctt tccgtggaaa
     2761 accttaattc tgagtccgcg gcgggtgtca cccgctttaa ggtccacggc taaagcgcgt
     2821 aacggagaga gtcatggcgg gcagcgtcaa caaggtcatt ttgattggca atttgggccg
     2881 cgatccggaa gtgcggcggc tgaactccgg cgattccgtc gtgaacttca gcgtcgcgac
     2941 gaccgaatcc tggcgcgata aagcgactgg tgagcgcaag gaccgcaccg aatggcataa
     3001 tgtcgtgatc ttcaatgaga atttggggaa gatcgcggag caatattgca agaagggctc
     3061 caagatctat ctcgaaggcc aattgcagac gcgcgaatat accgacaagg acggcaacca
     3121 gcgccgtacg acggaagtcg tcttgcagcg tttccgtggc gagctgacgt tgctcgacag
     3181 ccgcggtggc gcgcgtggcg aatccttcga cgatcgcggc ggcgcgggcg aagcctcaag
     3241 cagcggcggc agtttcggcc gttcctcgcc gatggactcc gacaagcggc caagccgcgc
     3301 tgcggaaatg atcgacgacg acattccgtt ttaatacgta tcagacgatc atgccgcggg
     3361 ggatcgctgc atgatcgtcg agaaaaaagt tttcgagctg cctgagtttc gcacgtccgg
     3421 cggccagctc atcaaaaata tccgcatcgg ctgggagagc tatggcacgc tcaatgaggc
     3481 gcgctccaat gcgatcctca tcgcgcatta tttttccggc accagtcacg ccgccggccg
     3541 ctacgccaaa gaagatgtct ttcccggcta ttgggacgct ttgatcgggc cgggcaaggc
     3601 gatcgatacc gatcgctatt tcgtcttctc atccgacacg ctggtcaatc tcaatgcgcg
     3661 cgatccgcat gtcgtcacga ctggcccggc gagcctcaat cccgacaccg gagcgtctta
     3721 cggcctcgat tttccgctgg taacgatccg cgatttcgcc gccgtgcaga aagcgcttgt
     3781 cgatcatctg ggcatctgca agcttcatgc ggtcatgggg ccgtcgatgg gcggcctgca
     3841 gacgttcgcc tgggccgcca cctatcccga catgatggat cgtatcgtcc ccgtcatttg
     3901 cgcgggcgaa gtggatgcgt ggctcattgc gtggcttggc ctttgggggg cgccgatcat
     3961 ggccgatccg gcctggaaaa acggcgccta cccgctcgat gaacagccgc tcgccggcct
     4021 gaaactggcg ttggaactcg tcacattgca ggccaatcat tggatctggg ccgatacgca
     4081 atttggccgc gccttcgccg aaccaggcgc tgatcccgga cttgcgctcg gcaatcgttt
     4141 caaggtcgag gcagcggtgt caggcatcgc tgccgaacgc gccaaaatat ccgacgccaa
     4201 tcatcttctt tatctcgtga aagccaacca gactttcgcc gccgggccaa ccggcacgct
     4261 tgccgatctc gaaaagataa aggccaaaac tctactgctc tattcccctg aagatcaggt
     4321 ctttcgcccc gacaaatccc gcgcgacggc ggccgcaatt gcgaagggcg gtgcgacggt
     4381 cgaaaccggc gaaatccacg gcccgttcgg acacgccaac ggacttttcg ccatggcgcc
     4441 gttgggtgat aggattaaag aattcctgac ggtgtagccg cctccctgcc cgcatccgtt
     4501 acgcgaatcg agcgccgatt tcggcgcgct aagcacccat tcgcaagcca tgcgcggcct
     4561 cacgccgcat ccgcctgtga gcattccacg gcactataat aagttgctga aatttatggg
     4621 tatttttgag ccgcttaaag ccgccacggc tggccttttt gggcgccgtt gattatatac
     4681 agatctcctc cgaagcttgg atgaattgcc ttggccgata ataacgaaaa cggcgccgga
     4741 ccctccgatt ccgacgtccg cccggtttct atttctgacg aaatgcgccg ctcctatctc
     4801 gattacgcga tgagcgtgat cgtcagccgc gctctgcccg atgtgcgcga cggcctgaag
     4861 ccggtgcacc ggcgcattct cttttcgatg tatgaaaaca attatctgcc agaccgcgcc
     4921 tataacaaat cggcgcgcgt caccggcgat acgatgggaa agtaccatcc gcacggcaac
     4981 ctcgcgattt atgatgcgct cgtgcgcatg gcgcagccgt tttcgatgca gctgccgctc
     5041 atcgacggcc agggcaattt cggctcggtc gatggagatc caccggcggc tgaacgctac
     5101 accgaggtgc gccttgcgac ctccgcgctg ccactgctcg acgatctcga taacgagacg
     5161 gtcgattttc aggcgaatta tgacggccgc gagcgcgagc ccgtcgttct tcccgcgaaa
     5221 tttcccaatc tgctcgtcaa tggcgcgggc ggcatcgccg tcggcatggc gacgaacatt
     5281 ccgccgcaca atcttggcga agtgaccgat gcggtattcg cactcatcga ccgtcccgag
     5341 atcaccgtcg aagagctgat cggtattatc cccgggccgg acttcccgac cggcggcagt
     5401 atcatcggcc gcggcggcat ccgcgccgct tatctcaacg gccgcggctc gatccttgtg
     5461 cgcgccaagg tcgaggtcga ggagatccgc aaggagcgcg aggcactcat cgtcaccgag
     5521 attccgtatc aggtgaacaa agccacgctg gtcgagaaaa tcgccgaact cgtgcgcgaa
     5581 aagaaggtcg aaggcatttc cgatctgcgc gacgagtccg atcgccaggg catgcgcatc
     5641 gtcatcgaac tgaagcgcga tgctgtatca gatgtcgtgc tgaaccagct ctggcgcttc
     5701 acgtcgctgc aatcgagctt cccggccaat atgatcgcgc tcaacggcgg acggcccgag
     5761 acgctgaacc tcaaggactt cctgcgcgcc ttcgtcgatt tccgcgaaga ggtcgtcacc
     5821 cgccgcacga aattcctgct cggcaaggcg cgcgatgcag cgcatctgca agtcggtctc
     5881 gccatcgccg tcgccaatat cgacgaggtg atccgcgtca tccgcacgtc gcccgacgcg
     5941 acgacggcgc gcgaagcctt gatgagccgc gagtggccag cgcgcgatat ggcgccgctg
     6001 atcgcgctga tcgccgatcc acgccatgtg ctgacggcag acggcatgtg ccgcctctcc
     6061 gaggcgcaag cgcgcggtat tctcgaattg cgcttgcagc gtttgacagc actcggccgt
     6121 gatgaaatcg gccaggcgct cgaaaagcat gccgccgaaa tcgccgatta tctcgatatt
     6181 ctgcgctccc gcgcgcggct ctttggaatc atcaaggacg agttgcaggc cgttaaactc
     6241 gcgcatgcaa caccgcgccg ctcgatcatc ctcgacagcg acgccgattt tgaggacgag
     6301 gacttcatcc agcgtgagga tgtcgtcgtc gtcgtttccc atgcgggcta catcaaacgc
     6361 gtgccgctct cgacctatcg cgcacagcgc cgtggcggca aaggccgcac tggcatgcag
     6421 acgcgcgacg aggatttcgt cgcaaagctg ttcgtcgctt cgacgcatac gccggtgctg
     6481 ttcttctcgt cgctcgggca ggcctataag gagaaagtct ggcgtctgcc cgcgtccgcc
     6541 ccgcaggcgc gcggcaaagc actagtcaat atcctaccgc tcgaacaggg cgaacgcatc
     6601 acaacgatca tgccgctacc ggaagacgaa gagcaatggg aaaagctcga cgtgattttc
     6661 gcgacgacac gcggcaccgt gcggcgcaac aaactctccg acttcgccca agtcaatcgt
     6721 gccggcaaga ttgccatgaa actcgatccc ggcgaagaga tcgtcaacgt gcagatctgc
     6781 aacgagagtg acgacgtgct gttgacgagc gcggacggcc aatgcattcg cttcccggtg
     6841 actgatgttc gcgtcttcaa ggggcgtgat tcgatgggcg tgcgcggcat tgcgcttggc
     6901 gagggcgatc gcgttatttc gcttgcgatc cttcaccaca tcgaagccga cagcggcgag
     6961 cgcacggcct atctcaagat gcgccgtgcc attgccggtg aggctgaggc cacagagccc
     7021 gcgaacgacg acgcgaatgg cgaagaggcg gccgccgccg cgctctcctc cgagcgttac
     7081 gcacaaatgt cggccgcgga ggaaatcatt ctgacaattt ccgcgaacgg ttacggcaag
     7141 cgcacgtcgt cctacgagta tcggatcacc gggcgcggcg gcaaaggcat cgtcgcgatg
     7201 gccgtgaacc agcgcaacgg gcaacttgtg gcctcaatgc cggtcgagga gaccgacggg
     7261 atcatgctgg tcactaacgg cggccagatg atccgctgcc cggtcgaggg catccgcgtc
     7321 gcgggccgca gcacccaggg cgtcatcgtc tttaatactg ccgaggacga gcgcgtcgtg
     7381 agcgtcgagt gcatcagcga ggatgaagcg gcggaaaatg gcgaggcttc ggacgaagcc
     7441 gcaagcgagt aatccacggc gcgggagagg gagcatattt gccccttctc gcgccctcgg
     7501 ctagaagtcc gcgatgacca tcaaggccct ctacaccggc acgttcgatc cgctgaccaa
     7561 cggtcacgtc gatattatcg cccgcgcagc gcgtttctgc gacgagcttg tggtcgcgat
     7621 cggtgtgcat ccgggcaaaa cgccgatgtt tgccgcggcg gagcgcactg atttcatccg
     7681 cgccgcttgc ggcggtctca cggcaaacca ttcctgcaaa ttatcggtgc tgacgttttc
     7741 cgggcttgcc gtgacggcgg cgcgcgagac tggcgcgaca cttctggtcc gcggcctgcg
     7801 cgacggcacc gatctggatt atgaagcgca gatgaccggc atgaatgcga tcatggcgcc
     7861 ggagatcgaa acgatttttc tgtccgcctc tccgcaagtt cgtcacattg ccgcgacatt
     7921 ggtgcgccaa atcgcactgc tcggtggcga tatcgcgcct ttcgtgcctg atgtcgtcgc
     7981 caaagccctt gccgtaaaac tcaacaacgc gccttaagct ttccaaggag gaaatatgcg
     8041 cctcgtccgc cgcagtcttc tggctgtcct cgccttcatg tttctgaccg cgccgctcgc
     8101 cgccgccgat ctcaaaaacg agatttatct ttatacgaag gacgggcggg tgacgctcct
     8161 gctgcggccg gaccttgcac cgaagaccgt tgagcagatc aagatcttga ccaagcgcgg
     8221 tttcttcaat ggcctcaagt ttcatcgcgt gatcgaaggc ttcatggcgc agacgggtga
     8281 tccgacgggc accggcaccg gcggctccga tctgccgaac gtgcccgccg aattcaacaa
     8341 agcgcatttc aagcgcggtt cgctcggcat ggcccgcgcc gatgatccga attccggcaa
     8401 ttcgcaattc ttcatctgct ttggcgatgc cggatttctt gacggccagt acaccaacta
     8461 cggcg
//
LOCUS       DASWOG010000713         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_90754,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000713 DASWOG010000000
VERSION     DASWOG010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90754"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGB93_14575"
     CDS             <1..803
                     /locus_tag="VGB93_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0147341.1"
                     /translation="RLDLPIKTDLSPQASEWRERILDLISAVIGGAAGIYALWLVIHF
                     VTNVLTPADIVHAFVLTCVTLIRVLALILLASLVWVPLGILIGLNPRLAERVQPLTQF
                     LAAFPANVLFPIAVVLIVRWALNPDIWLSFLIVFGTQWYILFNVVGGASAFPNDLREA
                     AATFHVRGWDWWTKVMLPAVFPYYVTGALTASGGSWNAAIVAEYVKWGKETVTAHGIG
                     AYIAKATEAGDYPRIVLGVAMMSIFVICLNRFFWRPLSRFAERRLQLS"
     gene            812..2140
                     /locus_tag="VGB93_14580"
     CDS             812..2140
                     /locus_tag="VGB93_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0147342.1"
                     /translation="MLDARSKPILEVKNVSQSYRTGAGEAGAQVLDNVSMNLTEGEIV
                     GLLGRSGSGKSSLLRIISGLARPTDGEVFFREKPVTGPYDSVSMVFQSAALFPWLSIL
                     ANVELGLRAKKVPEAEARPRALKAIDVIGLDGFESAFPKELSGGMRQRVGFARALVVN
                     PTILLMDEPFSALDVLTAETLRTDLLDLWVEGRIPIKSILMVTHNIEEAVLMCNRILV
                     LSSNPGRIAEEIHVHLPYPRNRLAPEFRQLVDRIYALMTRRQTAEPTTLGRDGTFPGL
                     GLGMALPEVSTNTMAGLMETLAAAPYSGHADLPPLADSLQMAIDDLFPVGETLQLLRF
                     AEFAEGDIKLTPAGMRFAEMDVDARKKLFGDHLLAYVPLATRIRNVLDERPTHWARAA
                     RFLEELEDYMSEEYAERTLKSIITWGRYGEIFAYDETSETFSLENPGETT"
BASE COUNT          379 a          675 c          621 g          470 t
ORIGIN      
        1 ggcggctcga tctgccgatc aaaacggatc tcagcccgca agccagcgaa tggcgcgaac
       61 gcatcctcga tcttatctcg gccgtcatcg gcggcgcagc cggcatttac gcgctgtggc
      121 tggtcatcca cttcgtcacc aacgtcctca cgcctgcgga catcgtgcac gcatttgtgc
      181 tgacgtgtgt cactctgatc cgggtgctgg cgctgatcct gctcgcatcg ctggtctggg
      241 ttcctttggg cattttgatc gggcttaatc cgcggctcgc ggaacgcgtt cagcctttga
      301 cgcagttcct cgccgccttc ccggccaacg tacttttccc gattgccgtc gtcctcatcg
      361 tccgctgggc gctcaatcca gacatttggt tgagcttcct catcgtcttc ggcacgcaat
      421 ggtacattct gttcaacgtc gtcggtggcg cgagcgcctt cccgaatgat ctgcgcgagg
      481 cggcagcaac ctttcacgtc cgcggctggg actggtggac gaaggtgatg ctgcccgcgg
      541 tgttccccta ttatgtcacc ggcgcgctga cggcctcggg cggctcttgg aatgcggcca
      601 tcgtcgccga atatgtcaaa tggggcaaag aaaccgtgac ggcgcacggc atcggcgctt
      661 atatcgccaa ggcaacggaa gccggcgatt acccacgtat cgtgctcggc gtcgccatga
      721 tgtcgatttt cgtaatttgt ctgaatcgct ttttctggcg tccgctctcg cgctttgccg
      781 agcggcgcct gcagctttcg tgaggcctct tatgcttgac gcgcgaagca agcccatcct
      841 tgaagtcaag aacgtcagcc aaagctatcg caccggcgcg ggcgaggccg gtgcgcaggt
      901 tctcgataac gtctcgatga atctgacgga gggcgagatt gtcggcctgc tgggacgttc
      961 gggttctgga aagtcgtcgc ttttgcgtat catctccgga cttgcgcgcc cgacagatgg
     1021 tgaagtcttt ttccgcgaga aacccgtaac ggggccttac gattccgttt cgatggtgtt
     1081 ccagagcgcg gcgttgtttc cttggctttc aatcctcgcc aatgtggaac tcggtctgcg
     1141 cgccaagaaa gtgccggagg ccgaggcacg gccgcgcgca ctcaaggcga ttgacgtcat
     1201 cggcctcgac ggctttgaat ccgcctttcc gaaagaactg tccggcggca tgcgccagcg
     1261 cgtcggcttt gcccgcgcgc tcgtcgtcaa cccgacaatc ctcttgatgg acgagccgtt
     1321 ctcggcgctc gatgtcttga ccgccgagac cttgcgcacc gacttgctcg atctttgggt
     1381 cgaaggtcgc atccccatca aatcgatttt gatggtgacg cataacatcg aggaggcggt
     1441 gctgatgtgc aatcgcatcc tcgtgttatc gtccaatccc ggtcgcatcg ccgaagagat
     1501 tcacgtccat ctgccctacc cccgcaatcg ccttgcgccg gaattccgcc aattggtcga
     1561 tcgaatttac gcgctcatga cgcggcgcca gaccgcggag ccgacaacgc tcggccgcga
     1621 cggcacgttc cccggccttg gtctcggcat ggcgctgccg gaagtctcga ccaacacgat
     1681 ggccggtctg atggagacgc tcgcggcagc gccctatagc ggccacgccg acctgccgcc
     1741 gctcgccgac tctttgcaaa tggcaatcga cgatctcttt cctgtcggcg aaacattgca
     1801 gcttttgcgc ttcgccgaat ttgccgaagg cgacatcaag ctgacgccgg cgggcatgcg
     1861 cttcgcggaa atggatgtcg atgcgcgcaa gaaactcttc ggcgatcatc tgctcgctta
     1921 cgttccgctg gcgacgcgca tccgcaacgt gctcgacgag cgcccgacac attgggcgcg
     1981 ggcggcgcgc tttctcgaag aactcgaaga ttatatgtcc gaagaatacg ccgagcggac
     2041 gttgaagagc atcatcacct ggggccgata cggcgaaatt ttcgcctacg acgagacctc
     2101 agaaaccttc tcgctcgaaa accctggcga aactacttaa gcccg
//
LOCUS       DASWOG010000714         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_219142,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000714 DASWOG010000000
VERSION     DASWOG010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219142"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VGB93_14585"
     CDS             complement(<1..1099)
                     /locus_tag="VGB93_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147343.1"
                     /translation="MEVIVLDNGLQSQGEHSYRLLLEVRDALSRRKITHRIFGMRDAD
                     VAAVADIGAIPWFTKSLYWSGRQRLPSLQNFVSDSLSLMRSLGTRGYHYSEEATARVL
                     NKTYRRDLERLPNDVWTRDNLIVVPAVSQNQILGLVQHLLALPASALPTVVCQLMFAP
                     AWTPWNRNGCHGEDYYRQAFELAAPLIGKSLFFTTENDALAQLYLRVFKLKTGLLPIV
                     TRIPHVASRPEGTIKLGFFGYSKSEKGFHLLPEAIGLCRARKLPAQFVVQIQHSNWER
                     ETVETEKALRRIPDIEIIDGALDADEYTRQINEVDATLLPYDPVRFGLRGSGIYTESV
                     AAGRPVVAAKGIYAALGIERGEAVGEIFAPYT"
     gene            1371..1952
                     /locus_tag="VGB93_14590"
     CDS             1371..1952
                     /locus_tag="VGB93_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0147344.1"
                     /translation="MLDKLSRVHWSIPLFIYTPVVALLAWLSFRNFSPFAVILATAAG
                     YAIWTLIEYFGHRYLFHAEFPGTWGTRVHFLIHGVHHDHPNDPLRLVMPPLLSGPIML
                     IALLVALVIFGRPLGYPMLMGFIIGYVAYDMVHYYVHHATPTTRLGRYLRQVHMLHHF
                     REPEGYFGVSAPYWDVVFKTRSASPARSARLRD"
     gene            complement(1994..2455)
                     /locus_tag="VGB93_14595"
     CDS             complement(1994..2455)
                     /locus_tag="VGB93_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147345.1"
                     /translation="MFAHRTLRQLTGWRTLREVYDRQVRWSVIRHTHEPLTFPLEPLV
                     SPLPAAMAAALAAPLLGLPTIAAFGISLTGWFLGELGLAWLKGWELSPYAPVAFLGRE
                     VLALAAWLRAWTTNDVVWQNGRFDVFVGARAAPRSSTLKLQPDAPVAREQI"
     gene            complement(2425..>2650)
                     /locus_tag="VGB93_14600"
     CDS             complement(2425..>2650)
                     /locus_tag="VGB93_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0147346.1"
                     /translation="SLLTLTVCLVAVTASAEEKFVPLFNGTNLDGWEGDPELWRVDGG
                     AIVGSTENKESKAWTRSGFARCSRIARCAS"
BASE COUNT          555 a          774 c          757 g          564 t
ORIGIN      
        1 cggtataggg cgcgaaaatt tccccgaccg cctcgccccg ttcgatcccg agcgcggcat
       61 aaattccttt tgcggcaacg acaggccggc ccgcggcgac agattcggta taaatgccgg
      121 agccccgcag accgaagcgt accggatcgt aaggcaaaag cgttgcgtcg acttcgttga
      181 tctgtcgagt atattcatcg gcatcgagcg ccccgtcgat gatttcgata tcggggatcc
      241 ggcgtagggc tttttccgtt tcgaccgttt cgcgctccca attggaatgt tgaatctgga
      301 cgacgaattg ggccggcagt ttccttgcgc ggcaaagccc gatggcttcg ggaagcagat
      361 gaaacccctt ctcggatttg gaataaccga aaaacccgag ctttatcgta ccttctggtc
      421 gagacgccac atggggtatc cgggtgacga tcggcagaag ccccgtcttg agtttgaaga
      481 cccgcagata aagttgcgcg agcgcgtcgt tttcagtcgt aaagaacaac gatttgccaa
      541 tcaagggcgc ggcgagctcg aatgcctgtc tataatagtc ttctccatgg cagccattgc
      601 gattccatgg cgtccaggcc ggtgcaaaca tcaactggca aacgactgtc ggcaatgccg
      661 aggcaggaag cgcgagcaga tgctgcacaa gaccaagaat ctgattttgc gagaccgccg
      721 gcacgacgat gagattatcc cgcgtccaga catcgttcgg caggcgctcg agatcgcgac
      781 gataggtttt gttgagaacg cgcgctgtcg cttcctcgct gtaatggtag ccgcgggtac
      841 ccagactccg catcagtgat agactgtcgg agacgaaatt ttgcagcgac ggcaatctct
      901 ggcggccgct ccagtaaagg ctcttggtga accagggaat tgcgccaata tcggcaacag
      961 cggctacgtc cgcgtcgcgc ataccgaaaa tacgatgggt tattttccgg cgcgacaaag
     1021 cgtcccgtac ctcaagaagc aggcggtagc tgtgctcgcc ctggctttga agaccgttgt
     1081 cgaggacgat cacctccatc cgagtatttt gccccgtagc ggaattcggc ggattcatat
     1141 aatttcgccg ttgaaactgt tttctgcggt acgattgcgc cgtgatcttg acggaaactc
     1201 aaatattcgc gccattgacg ctaatcacaa gggcgtgccc ctagtaaggg tgcgccgcag
     1261 tgcaatgcag gacgacgtcg aaacgatgag aaggttgctc aatgatatcc tctgaagccg
     1321 ggacaactgc cgaagcgctg cgcgcttccc cgcgcctatt cgacaaccca ttgcttgaca
     1381 agctttcgcg cgtgcattgg tcaataccgc tgttcatcta cacacccgtc gtggctttgt
     1441 tggcatggct gagttttcgt aacttttcgc ctttcgccgt cattctcgcc acggcggccg
     1501 gctacgcaat ttggactttg atcgaatatt tcgggcatcg ctatctgttc cacgccgaat
     1561 ttcctggcac atggggcacc cgtgtgcatt tcttgatcca cggcgttcat cacgatcatc
     1621 ccaacgatcc gttacgactt gtgatgccgc ccttgctgag cggtcccatt atgttgatcg
     1681 cgctcttggt ggcgctcgtg atttttgggc gcccgctcgg ttacccgatg ctcatgggtt
     1741 tcatcatagg gtatgtcgcc tacgacatgg tgcattatta tgtgcatcac gcgacgccga
     1801 cgacgcggct tgggcgttac ctgcgccagg tgcatatgct ccatcacttc cgcgaaccgg
     1861 aaggttattt cggtgtcagc gcaccttatt gggatgtggt ctttaaaacg cgctccgcat
     1921 cgccggcgcg gtcggcacgg ctacgcgatt gaggaagcag cgcagcccta tccaccccgt
     1981 gcggaacgag tcttcagatt tgttcccgag ccacaggggc gtcgggttgc agcttgagtg
     2041 ttgacgaacg cggggcggca cgggcaccga caaaaacatc gaagcgcccg ttttgccaga
     2101 cgacatcgtt cgtcgtccat gcgcgcaacc atgccgcgag tgccagcact tcgcgcccga
     2161 gaaacgcgac gggtgcataa ggcgacaatt cccagccttt aagccacgcg agaccgagtt
     2221 caccgagaaa ccagccagtc aacgaaatac cgaaggccgc aattgtggga aggcccagaa
     2281 gcggcgcggc cagcgccgcc gccattgctg ccggcaaagg gctgaccagc ggctcaagcg
     2341 ggaacgtcag tggttcgtgc gtgtggcgga tcacgctcca gcgaacctga cgatcataga
     2401 cttcgcgcaa agtccgccag ccggtcagct ggcgcagcgt gcgatgcgcg aacaccgtgc
     2461 gaagcccgat cgcgtccagg cctttgactc cttgttctcg gtcgagccga caatcgcgcc
     2521 gccgtcgacg cgccacaact cggggtcccc ctcccagcca tcgagattgg tgccattgaa
     2581 caggggtacg aatttctcct ctgccgaggc ggtgacagca acgaggcaaa ccgtcaaagt
     2641 taacagcgaa
//
LOCUS       DASWOG010000715         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_164330,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000715 DASWOG010000000
VERSION     DASWOG010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164330"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..687)
                     /gene="metW"
                     /locus_tag="VGB93_14605"
     CDS             complement(43..687)
                     /gene="metW"
                     /locus_tag="VGB93_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY0147347.1"
                     /translation="MANGTRTAVMLSQTARIDLLVVADMVVPKSRVLDVGCGDGTLLR
                     LLAEERGVDARGIELSQSGVNDCVAKGLSVIQGDADTDLADYPDDAFDYVVLSQTLQA
                     TRHPRRVLENMLRIGRHAIVSFPNFGHWRIRTQIALKGRMPVTKSLSAAWYETPNIHF
                     CTIRDFIGLVDEIGAKSERSIALDRQARPLRVGAPWWVLNLIGDQAVFELSRKK"
     gene            complement(690..1850)
                     /locus_tag="VGB93_14610"
     CDS             complement(690..1850)
                     /locus_tag="VGB93_14610"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0147348.1"
                     /translation="MSQLEADAPHSLVARFGADQPLKMDAGVDLGPLQIAYQTYGTLN
                     AAKSNAVLICHALTGDQHVASIHPITGKPGWWEVMVGPGKPIDTDRYFVIASNVVGGC
                     MGTTGPASINTATGRVWGLDLPLVTIRDMVRAQAMLIDYLGIETLFAVIGGSMGGMQV
                     LQWAASYPSRVFAAMPIATAAKHSSQNIAFHEVGRQAVMADPEWKQGRYLDAGASPTK
                     GLAVARMAAHITYLSEEALQRKFGRKYQDRAAPTFSFDADFQIENYLRYQGTSFVDRF
                     DANSYLYVTRACDYFDLAEDYGGTLAAAFKGSKTRFCVVSFDSDWLYPTSASRAIVHA
                     LNAAAASVSFVEIESDRGHDAFLLNVPDFIATTRGFLEAAARARGIAPASGA"
     gene            2112..2951
                     /locus_tag="VGB93_14615"
     CDS             2112..2951
                     /locus_tag="VGB93_14615"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY0147349.1"
                     /translation="MLERRPAPETLADLRDEIDRIDACLHEMLMQRGEIIDRLIAVKA
                     RSGPGSAFRPGREAEMMRRLVERHKGILPLDTVESIWRIIISTFTYVQADYSVHADIS
                     GGDAQMRDGCRFHFGFTVPYIPHPSSAEVICAVAAAPGDLGLVPVETVPAAGAWWQQL
                     ASPGAPKVIARLPFVERRDHPAGTPLFVLAKPVEDGAARDVVLYAAMVERWREIDESA
                     LRAHGVDVIAKAPQDRALLLAVTGALSPAALRETLASSGAEIAHLAEIGSHAARFDFV
                     RKT"
     gene            3026..>3275
                     /locus_tag="VGB93_14620"
     CDS             3026..>3275
                     /locus_tag="VGB93_14620"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187168.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0147350.1"
                     /translation="MTSSDIAATPQPRASVLAIEPYVPGKASAATKGKIYKLSSNESA
                     LGPSPRAIEAFQSAAGSLSIYPDGSGHTLRAAIAKRHGI"
BASE COUNT          623 a         1057 c         1016 g          579 t
ORIGIN      
        1 tggattgctt cgtcgcctac gtctcctcgc aatgacgagg tgctacttct tccgcgacag
       61 ttcgaagacc gcctgatcgc caatcaggtt caagacccac cacggcgcgc cgacgcgcag
      121 tggacgggcc tgccgatcga gcgcgattga gcgctcgctc ttagcgccga tctcgtcgac
      181 gaggccgatg aagtcgcgga tcgtacaaaa gtgaatgttc ggcgtctcat accacgcggc
      241 tgaaagactt ttcgttaccg gcatccggcc cttgagcgcg atctgggtgc ggatgcgcca
      301 atggccgaaa ttcggaaacg acacgatggc gtggcgaccg attcgcagca tgttttcgag
      361 cacccggcgc ggatggcgcg tcgcctgcaa agtttgcgac agcaccacgt aatcgaatgc
      421 atcgtccgga taatcggcaa ggtcggtgtc ggcgtcgccc tggatcaccg aaagaccctt
      481 ggcgacgcaa tcgttcacgc cgctttgcga aagctcgatg ccgcgcgcat caacgccgcg
      541 ctcctcggca agaaggcgca acaacgtgcc gtcgccgcag ccgacatcga gcacgcggct
      601 cttcggcacg accatgtcgg cgacgacgag aagatcgata cgcgccgtct gcgacagcat
      661 gacggcggtg cgcgtgccat tcgccatcat taggccccgg acgcgggcgc gataccacgc
      721 gcgcgagcgg ccgcttcgag aaagccgcgt gtcgtggcga tgaagtccgg cacgttcaag
      781 agaaacgcgt cgtggccgcg atcagactca atctcgacga aggagacgga ggccgccgcg
      841 gcgttcagcg catggacgat cgcgcgcgac gccgacgtcg ggtacagcca atcggaatcg
      901 aacgagacga cgcagaagcg cgtcttcgat cccttgaacg ccgcggcgag cgtgccgccg
      961 taatcctccg cgagatcgaa ataatcgcag gcgcgcgtca catagagata tgaatttgca
     1021 tcgaaccgat caacgaaact cgtgccctga tagcgcagat aattctcgat ctggaaatcg
     1081 gcgtcgaacg aaaaggtcgg cgcagcacga tcctgatact tgcggccgaa cttgcgttgc
     1141 aacgcttcct cggaaagata ggtgatatgc gcagccatgc gtgcgacggc gaggcccttc
     1201 gttgggctcg cgccggcgtc gagatagcgc ccctgcttcc attcgggatc ggccatcacg
     1261 gcctggcgcc cgacttcgtg gaaggcgatg ttctgcgagg aatgtttcgc ggcggtcgcg
     1321 atcggcatgg cggcgaaaac ccgtgacgga tagctcgctg cccattgcag cacctgcatg
     1381 ccacccatcg agccgccgat gaccgcgaac aatgtttcga tcccgaggta atcgatcagc
     1441 atcgcctgcg cgcgcaccat atcgcggatc gtgacgagcg gcagatcgag gccccagacg
     1501 cggccggttg ccgtattgat cgacgccggc cccgtcgtgc ccatacagcc tccgacgaca
     1561 ttcgaggcga tgacgaaata gcggtcggtg tcgatcggct tgcccggacc gaccatgacc
     1621 tcccaccagc cgggcttgcc ggtgatcgga tggatgctcg cgacatgctg gtcgccagtc
     1681 agcgcatggc agatcaggac agcgttggat ttcgccgcgt tcagcgtccc ataggtctga
     1741 taggcgattt gcagcggccc gagatcgacg ccagcgtcca ttttcagcgg ctggtcggcg
     1801 ccgaaacgcg ccacgaggct atgcggggca tccgcctcga gctggctcag agctttctgg
     1861 ttgggaacaa ttgtcacgat caaccttcgt tcgctttgac gaacgccacg tccggtttat
     1921 agaagcgcgc tgcggcgcct gtcaaggcgg ccagacgcgc ggcgctgcca tccgacgagc
     1981 agatttaagt acgccccgac cggagcgcca gcgtgcgccc cgcaagccag agctgctccc
     2041 cgaaaaggga aaagcgtttc gccgggggca aattctcttt aagagaggtc cgcaaagaag
     2101 ccatgtccat gatgcttgaa cgacgccctg ctccagaaac tttggccgat ctgcgtgatg
     2161 agatcgaccg tatcgacgcg tgcctgcacg aaatgctgat gcagcgcggc gagatcatcg
     2221 accggctgat cgccgtcaag gcccgctccg gccccggcag cgccttccgg cccggtcggg
     2281 aagccgaaat gatgcgtcgg ctggtggagc gccataaagg catcctgccg ctcgataccg
     2341 tcgagagcat ctggcggatc atcatttcga ccttcaccta tgtgcaggcg gactacagcg
     2401 tgcatgccga catttctggc ggcgatgcgc agatgcgcga cggctgccgt ttccatttcg
     2461 gctttaccgt cccctatatt ccgcatccga gcagcgctga ggtgatctgc gccgtcgccg
     2521 ccgcgcccgg cgatctcggc ctcgtgcccg tcgaaacggt tcccgccgcc ggggcttggt
     2581 ggcagcaatt ggcgtcgccc ggcgcaccaa aggtcattgc gcgcctgccc ttcgtcgagc
     2641 gtcgcgacca tccggccggc acgccgctct tcgtgcttgc caagccggtt gaggacggcg
     2701 cagcgcgtga tgtcgtgctt tacgccgcga tggtcgagcg atggcgcgag atcgacgagt
     2761 cagctttgcg cgcacatggc gtggacgtga tcgccaaagc accgcaggac cgcgccctgc
     2821 ttctcgctgt gacgggcgcg ctttcacctg ccgcactgcg cgagaccttg gccagcagcg
     2881 gcgcggagat cgcgcatctg gccgaaatcg gcagccacgc cgcgcgtttc gatttcgtca
     2941 gaaagacctg agacgcggat tcgacctgtc gtccgcacgc ttattgttcg cacgcttgga
     3001 gcgcgattat ttggagcccg caaaaatgac gtcttccgac atcgccgcca cgccgcagcc
     3061 ccgcgcgagc gtattggcga tcgaacccta cgtccccggc aaggccagcg ccgcgacgaa
     3121 aggcaaaatc tacaagctct cgtcgaatga atcggcgttg gggccgagcc cgcgcgcgat
     3181 cgaagccttt caatcggccg ccggctctct gtcgatctat cccgatggct ccggccacac
     3241 attgcgcgcg gcgatcgcca agcgccacgg catcg
//
LOCUS       DASWOG010000716         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_292887,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000716 DASWOG010000000
VERSION     DASWOG010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292887"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGB93_14625"
     CDS             <1..242
                     /locus_tag="VGB93_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0147351.1"
                     /translation="GAFDYLAKPADADDIYAALMATHHDKAEVPENPMSADRVRWEHI
                     QRIYELCGRNVSETARRLNMHRRTLQRILAKRAPR"
     gene            complement(243..725)
                     /locus_tag="VGB93_14630"
     CDS             complement(243..725)
                     /locus_tag="VGB93_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEY0147352.1"
                     /translation="MSRVGSLAVVTDGRQSEMALAIARGTRRLLRNLRFSTVTELPLA
                     SGRRADVVALAADGTIVIVEIKSSLADFRADQKWPDYRAHCDRFYFAISDNLAPEIMP
                     AEAGLIVADAFGAAVLREAPEHRLPASTRRAMLMRFAHAAAHRLHGLTDPEILSFGPL
                     "
     gene            complement(733..996)
                     /locus_tag="VGB93_14635"
     CDS             complement(733..996)
                     /locus_tag="VGB93_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY0147353.1"
                     /translation="MPDTRSSVFNDFSKLVSDATEMAQGVRREAESVVKAQAERLLSA
                     MDIVSREEFEAVRDMAIAARDENDRLRKRIETLEAKLGATAGA"
     gene            1247..>1598
                     /locus_tag="VGB93_14640"
     CDS             1247..>1598
                     /locus_tag="VGB93_14640"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0147354.1"
                     /translation="MRHLVGKDSLWRRVPHPSQASIHDLVVYAAFGIILGGRLGYVLF
                     YNLPVYLAHPLEIFAVWKGGMSFHGGLIGAMLGIILFARRVGVPILAVMDATAASVPI
                     GLSLGRLANFIKPEL"
BASE COUNT          277 a          542 c          455 g          324 t
ORIGIN      
        1 tgggcgcttt cgattatctc gcaaagcccg cggatgccga cgacatttac gctgcgctga
       61 tggccacgca tcacgacaag gccgaagtgc ccgaaaatcc gatgtcggcc gatcgcgtgc
      121 gctgggagca catccagcgc atctacgagc tttgcggccg caacgtttcg gagacagcac
      181 gtcggctcaa catgcaccgg cggacattgc agcgcattct cgccaaacgc gccccgcgct
      241 aactaaagcg gcccgaagct caaaatctcc gggtcggtca gcccgtgcag ccggtgtgcg
      301 gcggcatgcg cgaagcgcat cagcatggct cggcgcgtcg aggccggaag acggtgctcg
      361 ggcgcctcac gcaaaaccgc cgcaccaaat gcgtcggcga caatcaaccc ggcttccgcg
      421 ggcatgatct ccggcgcgag attgtctgaa atcgcgaaat aaaatcggtc gcaatgcgcg
      481 cgataatccg gccatttctg atcggcacga aaatccgcaa gcgacgattt gatctcgacg
      541 atgacgatcg tgccgtccgc ggccaaggcg accacatcgg cgcgccggcc actcgccagc
      601 ggcagttctg tcacggtcga gaaacgcaaa ttgcgcagca gccgccgcgt gccgcgggca
      661 atcgccaagg ccatttccga ttggcggcca tcggtgacga ccgccagaga cccgacgcgc
      721 gacatcgacg tctcaggcgc ccgcggtcgc gccaagtttg gcttcaagcg tttcgattcg
      781 cttcctcagc cggtcgttct catcgcgcgc ggcaatcgcc atgtcgcgca cggcttcgaa
      841 ttcctcgcgg ctgacgatat ccatcgcgga cagaagccgc tccgcctgtg ctttgacaac
      901 gctttccgcc tcgcggcgca cgccctgcgc catctcggtc gcgtcgctca ccagcttgct
      961 gaaatcattg aatacgctgc tgcgggtatc gggcatcgcg cgctccgtgg gtcatccgtt
     1021 ctctttatgt tgcgcgtttt cacgtttgcc gcaagccttg acctcaagcc ttgaccttcc
     1081 cgctcgcgcg tcgcatcacc gccgcagaga tcacactggc tcggggtgga tgctgcttct
     1141 tattccctat ccagtcatca atccgatcct ggtgaatatt cgcccgctgc cgatcctctg
     1201 gtatgcgctg ccctagatcg tcgggatcat ctcgggctgg gcctatgtgc gccatctcgt
     1261 cggcaaggat tcgctttggc ggcgcgtgcc gcatccaagc caggccagca ttcacgatct
     1321 cgtcgtctat gccgccttcg gcatcattct cggcggccgc ctcggctacg tgctcttcta
     1381 taatctgccg gtctatctcg cccatccgct cgaaatcttc gccgtctgga agggcggcat
     1441 gtcgtttcac ggcggcctga tcggcgccat gctgggcatc atcctctttg cacgccgcgt
     1501 cggcgtgcca attctggcag tcatggatgc aacggcggca tcggtgccga tcggcctctc
     1561 gctcggcagg ctcgccaatt tcatcaagcc tgaactct
//
LOCUS       DASWOG010000717         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_365846,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000717 DASWOG010000000
VERSION     DASWOG010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365846"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VGB93_14645"
     CDS             complement(<1..757)
                     /locus_tag="VGB93_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0147355.1"
                     /translation="MIQFSDLGLSEVLLRALQQEGYVHPTPIQAQAIPSLLQGRDLLG
                     IAQTGTGKTAAFALPILNRLLHDKQRPAPRSVRALVLAPTRELAVQITESFRTYGKFG
                     HLSVATVVGGVSSVGQIKALARGVDILVATPGRLLDHLSTGNVRLDMTQVVVLDEADH
                     MLDLGFIIPIRQIISRMPKERQSLFFSATMPKEIAGIAADFLRDPVEVKVAAVAATAE
                     LVAQEVYLVDAAAQRGLLVELLAQPAFQRTIVFA"
     gene            complement(982..>1859)
                     /gene="cobS"
                     /locus_tag="VGB93_14650"
     CDS             complement(982..>1859)
                     /gene="cobS"
                     /locus_tag="VGB93_14650"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEY0147356.1"
                     /translation="ELLETRWIRRKRTLDKANAPSETAEMNFPNVREVLTELAADLVA
                     CLRFYSRLPIPPLGFESNPHGAEISARVKMLPLAGALIGSLAALVLLLVTELGLTPQI
                     AAIFAIFTLIIVTGAFHEDGLADFADSTGGATPEQRLIIMKDSRIGTFGMLALLASVL
                     LRIFTVSILARHNIALAGLILIATGAVSRTLGLLPLALLPPARAEGAGFSARSDQPPL
                     RTAALLAFLVSLLPLLAGASFWRVLGGLLISAAAAYGITALARRQLGGQTGDVAGAAQ
                     QAAEIAAYLVFAARI"
BASE COUNT          383 a          550 c          619 g          307 t
ORIGIN      
        1 gggcgaagac gatggtacgc tgaaaggcag gctgcgccag caattcgaca agcaggccgc
       61 gctgtgcagc cgcgtcgacg agatagacct cctgcgcgac gagctcggcg gtcgcagcca
      121 ccgccgcgac ttttacctcg actggatcgc gcaaaaagtc ggcggcaatg ccagcgattt
      181 ccttcggcat cgtggccgag aagaacaggc tctgacgctc cttcggcatc cgcgagataa
      241 tctggcggat cggaatgatg aagccgagat cgagcatgtg gtcggcctca tcgagcacaa
      301 ccacctgggt catatcgagc cggacgttgc cggtggagag atgatcgagg agccgtccgg
      361 gggtcgccac gaggatgtcg acgccacggg cgagcgcctt aatctggccg acgctgctca
      421 cgccgccgac gactgtcgcg acgctcaaat ggccaaactt gccataggtg cggaaactct
      481 cggtgatctg gactgccagc tcgcgagtcg gtgccagcac gagcgcgcgg acgctgcgcg
      541 gcgcgggccg ctgtttgtcg tggaggagac gattgaggat cggcaaggca aacgcggcgg
      601 ttttgccagt gcccgtctgg gcaataccaa ggagatcgcg gccctggagt agggacggaa
      661 tcgcctgcgc ctggatcggc gtcggatgta catagccttc ctgttggagg gcgcgaagca
      721 gcacctcgga gaggccgagg tcggaaaatt gtatcactga atgtctttct tcaaggcaga
      781 ccccgcgccg cgaccacgaa ggacgcgcgc gaactcgtgc gaggggttga gaccgccccg
      841 cgtatcgggc tgccggctgg atgtggggag gcgctcaaca gatcgccgat cttttgatca
      901 gcctacgcga ctggagcaca tcatgctgcg atgcagcgat tcaggcttta caggttttgc
      961 tccgccgcgt caacgaaggg ctcatatccg cgcggcaaag acgagatagg ccgcgatctc
     1021 ggctgcctgc tgtgcggcgc ctgcgacatc accggtctgg ccgccgagct ggcgccgcgc
     1081 cagcgccgtg atgccatagg ccgcggcggc actgatcaag agccccccga gaacgcgcca
     1141 aaaactggcg cccgccagca gcggcaaaag gctgacgagg aaagcgagga gggccgctgt
     1201 ccgcaacggt ggctgatcgc ttcgggcgga aaatcctgcg ccttccgcgc gcgcaggcgg
     1261 caacaaagcg agcggcagaa gccccagcgt gcgagaaaca gcgcctgtcg caatcaggat
     1321 gagaccggcc aaagcgatgt tgtgccgcgc cagaatcgaa acggtgaaaa tcctcagcag
     1381 gacgctggcg agcaatgcga gcatcccaaa agtgccgatc cggctgtctt tcataatgat
     1441 cagtcgttgt tccggcgtag cgccacccgt cgaatcggcg aaatcggcga ggccgtcttc
     1501 atgaaaggcg ccggtgacga tgatgagcgt gaagatcgcg aagatcgcgg cgatctgcgg
     1561 cgtcagcccg agttccgtga cgagcagcaa gacaagcgct gctaggctgc cgatcagcgc
     1621 acctgcaagc ggcagcattt tcacccgcgc ggatatttct gcgccatggg gatttgattc
     1681 gaagccgagc ggggggatcg gcagccggga gtaaaagcgc aggcacgcca cgagatcggc
     1741 ggcgagctca gtcaggactt cgcggacgtt gggaaaattc atctccgcag tttcagacgg
     1801 tgcgttcgct ttgtcgagcg tccgttttcg tcgaatccac cgcgtttcca gcaattcta
//
LOCUS       DASWOG010000718         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_73076,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000718 DASWOG010000000
VERSION     DASWOG010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73076"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGB93_14655"
     CDS             complement(<1..1034)
                     /locus_tag="VGB93_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0147357.1"
                     /translation="MVSKATAAARAESNGQKDPAPASPSFEEHISGADSAKLLSAMLA
                     MRAGDFSVRLPGNLTGLTGKVADIFNEIIAANQQMATQLASVGEAVGRDGRIRNRVRF
                     GISTGAWADMETSINTLIDDLLWPTTSVTRTISAVAKGDLLQPVPLDVDGRPLKGDFL
                     RSATIANAMIEQLSVFTSEVTRVAREVGTDGKLGGQAKVKNATGVWKDLTESVNSMAS
                     NLTGQVRNIAEVTIAVASGDLSKKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVA
                     REVGTEGRLGGQAQVPGVAGTWKDLTDNVNLMASNLTAQVRNIAEVSTAIAKGDLSKK
                     ITVNVRGAIL"
     gene            complement(1185..1739)
                     /locus_tag="VGB93_14660"
     CDS             complement(1185..1739)
                     /locus_tag="VGB93_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY0147358.1"
                     /translation="MQPQSLDDIVHVIQVALTPVFLLSGVASLLAVFATRLGRVADKV
                     DALSDALDSASQAEREQILVRLGYLRRRSHALDAAVVLGALAGVSTSGAALMLFVGTL
                     RNGTGSTLMFLAFGAALVLTIGALTAFLIEMLMASRGIRAIVAAEKAQQEGPEPDPEP
                     EVAEHGASAAPNLPDSAGSGAPVD"
BASE COUNT          335 a          556 c          536 g          324 t
ORIGIN      
        1 tcaaggattg cgccgcggac attgacggta attttcttgg agaggtcgcc cttcgcgata
       61 gccgttgaca cttcggcaat gttgcggacc tgagctgtca gattcgaggc catcaggttc
      121 acgttgtcgg tcaggtcctt ccacgtgccc gcgacgcccg gcacttgcgc ctgtccgccg
      181 agccggccct cggtaccgac ttcgcgcgca acgcgcgtca cttccgaggc gaaggagcgc
      241 aactgatcga ccatcgtgtt gatggcttct ttgagctgca gaatctcgcc gcgcacgtcg
      301 acggtaattt ttttcgacag atcgcccgag gcaacggcga tcgtgacttc cgcgatattt
      361 cgtacctggc cggtcaaatt cgaggccatg gaattcacgg actcggtcaa gtccttccag
      421 acgccggtcg cgtttttaac tttcgcttgc ccgccaagct tgccgtccgt tccgacttcg
      481 cgcgcgacgc gcgtcacctc ggaggtgaag accgacaatt gctcgatcat tgcattggca
      541 atcgtagcgg aacgcagaaa atcgcccttc agcggccgcc cgtcgacgtc gagcggcacc
      601 ggctggagca gatcgccctt cgcgacggcg gagatcgtgc gcgtcaccga cgtcgtgggc
      661 caaaggaggt cgtcgatcag cgtgttgatc gaggtttcca tatccgccca ggcgccggtc
      721 gaaatgccaa accgcacccg gttgcgaata cgtccgtcac ggccgaccgc ctcgccgacg
      781 ctcgccaatt gcgtggccat ctgctggttc gccgcgatga tttcgttgaa aatatctgcg
      841 actttgcccg tgaggccggt gagattgccg ggcagccgca cgctgaaatc gcctgcgcgc
      901 attgccagca tggcgcttag aagcttggcg gaatccgctc ccgaaatatg ctcttcaaac
      961 gagggacttg cgggcgccgg gtctttttgg ccgttggact cagcccgcgc cgccgccgtt
     1021 gctttcgaca ccatcaatgt aaaccccgcc ctctaacgcg cgaggaatcg cagccctgcg
     1081 cggcagatac gcccccgtag cgaaacgcgc agcgaaccat aaagttccgg cgatccggac
     1141 cgagggagtc accggatgat gcccgcaggc aggcaggtgc cggtttaatc gaccggcgcg
     1201 cccgaacccg cgctgtccgg aaggtttggc gcggcgctcg cgccgtgctc agctacctcc
     1261 ggctccgggt ccggttcagg tccttcctgc tgggcttttt cggcggcaac gatcgcgcgg
     1321 atgccgcggc tcgccatcag catttcgatc aggaaagccg tcaaagcgcc tatcgtgaga
     1381 acaagcgcgg caccgaacgc cagaaacatc agcgtcgaac cggttccgtt tcgcaaagtc
     1441 cccacaaaga gcatgagagc ggcgccgctc gtcgaaacgc cggcgagggc accgaggacg
     1501 acagcggcat cgagggcgtg ggatctacgg cggagatagc caagccgtac aagaatttgc
     1561 tcgcgttcgg cctgcgaagc ggagtccaaa gcgtccgaca aggcatcgac cttgtccgcc
     1621 acgcggccaa gcctcgtcgc gaagactgcg agaagcgacg cgacgccgga caaaagaaag
     1681 accggcgtaa gtgcgacttg gatgacgtga acaatgtcgt cgagcgattg gggctgcatg
     1741 atcgcgcctg c
//
LOCUS       DASWOG010000719         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_219668,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000719 DASWOG010000000
VERSION     DASWOG010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219668"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VGB93_14665"
     CDS             <1..540
                     /locus_tag="VGB93_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0147359.1"
                     /translation="RVSVMALLLREQKPVVDFLDDLKRDPPVRVSGTAIFLDAKASGI
                     PRALLNNIKFNRVIHERVVLLTVVTREQPRIAEAKRLTVTPVCEGIVRIVAQVGFMEK
                     PNIQEILRDAEAYGVPYEPERTTFFLSREELLPGQRSDLPPLRRKAFMQMAKNAQVIA
                     DYYGLPPSRVVEIGTRLEI"
     gene            complement(529..1191)
                     /gene="rpiA"
                     /locus_tag="VGB93_14670"
     CDS             complement(529..1191)
                     /gene="rpiA"
                     /locus_tag="VGB93_14670"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY0147360.1"
                     /translation="MSSSTEKKRRAAEAAVSYIEEGMVLGIGTGSTVNQLIEVLKDRK
                     IQLKGAVSSSNQTADALRKARIPELDLNSEGDLSLYIDGADQATANGTLIKGGGAALT
                     REKVVAAAAAKFVCIIDDSKLVDVLGSFPLPVEVVPMARSYVARQLVKLGGQPVWRTG
                     VVTDNGNVILDVHNLRLTDPLGFETAVNQIAGVVTVGLFAHRRADVLLVAGDDGVRTL
                     DL"
     gene            complement(1432..>1784)
                     /locus_tag="VGB93_14675"
     CDS             complement(1432..>1784)
                     /locus_tag="VGB93_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY0147361.1"
                     /translation="MLRDSMKKGDSAFFYHSSCDVPGIAGIVEVVKGGYPDVTAFDAK
                     DHHYDPDSDPSQPRWYVVDVKLIRKFSRVITLDELRKHASSSLEDLLVLRRGNRLSVT
                     PVTKKDWDFILELE"
BASE COUNT          354 a          526 c          526 g          378 t
ORIGIN      
        1 cgtgtatcag tgatggcgct attgctgcgc gagcagaagc cggtggtcga tttcctcgac
       61 gacctgaagc gcgatccgcc ggtacgcgtc tcgggtaccg cgatttttct cgacgccaag
      121 gcgtcgggca ttccgcgggc cctgctcaac aacatcaagt tcaaccgcgt gattcatgag
      181 cgggtggtct tgctgaccgt cgtgacacgc gaacagccgc gcatcgcgga ggcgaaacgg
      241 ctgaccgtga cgccggtatg cgaagggatc gtgcggatcg tggcgcaggt ggggttcatg
      301 gagaaaccca acatccagga gattctgcgc gacgcggaag cctatggcgt cccctacgaa
      361 ccggagcgga ccacgttctt cctgagccga gaagaactct tgccgggaca gcgctcggat
      421 cttccgccgc tgcgccgcaa ggccttcatg cagatggcga agaatgcgca ggtcatcgcg
      481 gactactacg gtttgccgcc gagccgcgtg gtcgagatcg ggactcgctt agagatctag
      541 cgtccgtacg ccgtcgtcgc ccgcgacgag gagcacatca gcgcgccgat gtgcgaaaag
      601 accgacggtg actacccccg cgatctgatt gacggcggtc tcaaacccca aaggatcggt
      661 cagccgcagg ttatggacat cgaggatgac atttccgtta tccgtcacca cgccggtacg
      721 ccacaccggc tgacccccaa gcttgaccag ttggcgcgcg acgtaggagc gcgccatcgg
      781 caccacttcg acgggcaagg ggaacgaccc caagacatcg accagcttgc tgtcgtcgat
      841 gatgcaaacg aattttgcgg cggcggcggc gaccaccttc tcgcgcgtca gggctgctcc
      901 gccgcccttg atgagcgtgc cgttcgccgt cgcctggtcg gcgccgtcga tgtaaagact
      961 gagatcccct tccgagttca gatcgagttc cggaatgcgg gctttgcgca atgcgtcggc
     1021 agtttgattg gagctcgaga ccgcgccttt gagctggatt ttgcggtctt tcagcacttc
     1081 gatgagctga ttgacggtgg agccggtacc gatgccgagc accatgccct cttcgatgta
     1141 ggacaccgcg gcttcggccg ctcgacgctt cttttcagtg gatgatgaaa ttttccaatc
     1201 ctcaaaaaca actgtgcccg cttacgcggg cacagttgat tactttcgaa ggcttacttt
     1261 ttcttagcgg cctttttctt agctaccttc ttcttcgcct tctttgcttt cgctgccatg
     1321 atagaaactc cgttgtcggg ttagtccggg gaccgagtat atacacgaat aattaattta
     1381 caagcaatac gtcgcgagac ggtgcgacat gtggtagcga tgtgagcgat ttcactctag
     1441 ctcgagaatg aaatcccaat ccttcttggt caccggcgtc accgacaagc gattccctcg
     1501 gcgcaaaacg agcaggtcct cgagcgatga ggaggcatgc ttgcgcagct catcgagcgt
     1561 gatgacgcgc gagaatttcc ggatcaactt cacgtcgacc acataccagc gcggctgact
     1621 cgggtcgctg tcgggatcgt agtgatgatc cttggcgtcg aaggcggtga catccgggta
     1681 gccgcccttc accacctcga cgatgcccgc gatgcccggc acgtcgcagc tcgaatggta
     1741 gaaaaatgcg gaatcgcctt ttttcataga gtctcgcagc atgt
//
LOCUS       DASWOG010000720         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylovirgula sp. isolate SMAG_U3434 k141_73233,
            whole genome shotgun sequence.
ACCESSION   DASWOG010000720 DASWOG010000000
VERSION     DASWOG010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959253
            Sequence Read Archive: SRR10968229
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylovirgula sp. (soil metagenome)
  ORGANISM  Methylovirgula sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylovirgula.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:26:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 21 of 82
            Pseudo Genes (incomplete)         :: 28 of 82
            Pseudo Genes (internal stop)      :: 41 of 82
            Pseudo Genes (multiple problems)  :: 7 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Methylovirgula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73233"
                     /isolate="SMAG_U3434"
                     /isolation_source="soil"
                     /db_xref="taxon:1978224"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66637735 N 14.70987936 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1673
                     /gene="tsaE"
                     /locus_tag="VGB93_14680"
     CDS             129..1673
                     /gene="tsaE"
                     /locus_tag="VGB93_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0147362.1"
                     /translation="MAPQRTQTAVWQIVLPDEAATATLAIELTHWLKPGDLLTLSGDL
                     GAGKTAFARALVRSLTGDAELDVPSPTFTLMQIYDGLDFPIVHADLYRIRAPEELEEL
                     GWDEAGEGALVLVEWPDRAGGFLRADRLDLAFSLEPEQGPNFRNAILTGYGSFASRLG
                     ISRAIHQVLRASGFEDAERQFMLGDASTRAYERLTKADGTTAVLMIAPPRPDGPPIRF
                     GKSYGTLARLAENIRPYVAIDTGLRAEGFSAPEIYAADLDVGVAVIEDFGADGVTDGG
                     AIITERYAESVALLARLHQLTLPDALPIDGVNTYRVPSYDLDALSIETELLIDWYAPH
                     VAKASLTSGAKASFVNLWRHVLRDIVSGPQTWTLRDFHSPNIIWLGERHGSARVGLID
                     FQDCVLGHPAYDVVSLLQDARVNVPADVELKLLGHYMQLRRIADANFDMAGFARAYAV
                     LGAQRATKILGIFARLDKRDHKPQYLAHLPRIEENLAKDLAHPLLSGLKGWYEAHLPQ
                     IFQRGA"
     gene            1751..>2024
                     /locus_tag="VGB93_14685"
     CDS             1751..>2024
                     /locus_tag="VGB93_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0147363.1"
                     /translation="MPDKAMVFAAGLGTRMRPLTETVPKPLIKVGGRPMIDHLLDRFA
                     QAGVSTAIVNVHHLADQIETHLDGRSAPHVVISDERDLLIDQGGAIK"
BASE COUNT          360 a          643 c          610 g          411 t
ORIGIN      
        1 ctatagacgt ggccatatag acaaagcggt gggtatcgca ctatccacgg cctgccgagg
       61 ttgatcttgg gaattcgctt cgcgttgact gcaggatcgg gccgttttcg atgaggggta
      121 tctaggcgat ggcgccacag cggacgcaga ccgcagtctg gcagatcgtc cttcccgatg
      181 aagcggcgac ggcgacgctt gccatcgaac tcacgcattg gctgaagccg ggcgaccttt
      241 tgaccctcag cggcgatctc ggcgccggca agacggcctt tgcgcgggca ttggtccgca
      301 gcctcaccgg cgacgccgaa ctcgacgtgc ccagcccgac attcacgctg atgcaaatct
      361 atgacggctt ggactttccg atcgtccatg ccgatctcta ccgcatccgc gcgccggagg
      421 aattggaaga gctcggctgg gacgaagccg gcgaaggtgc acttgttctc gtcgaatggc
      481 cggatcgcgc gggtggattt ctgcgggctg accggctcga cctcgccttt tcgctggagc
      541 ccgaacaagg ccctaatttt cgtaacgcga ttctcacggg ctacggcagt ttcgcctcgc
      601 gcctcggcat ctcgcgtgcc attcatcaag tgcttcgcgc ctcgggcttc gaggatgccg
      661 agcggcaatt catgctgggc gatgcctcga cgcgcgccta cgagcgtctg accaaagccg
      721 acggtacgac agcggtgctg atgatcgcgc cgccgcgccc ggacgggccg ccaatccgtt
      781 tcggcaaatc ctatggcact ttggcgcggc tcgccgaaaa catccgcccc tatgtcgcca
      841 tcgatacggg cctgcgcgcc gaagggtttt cagcgcccga aatctatgcg gcagatctcg
      901 atgtcggcgt cgcggtgatc gaagatttcg gcgccgatgg cgtgaccgat ggcggcgcca
      961 tcatcacgga gcgttatgcc gaatccgtcg cgctgctcgc gcggctgcat caattgacgc
     1021 tgccggatgc gctgccgatc gacggcgtaa acacctatcg cgtcccgtct tacgacctag
     1081 acgcactctc gatcgagacc gagcttctca tcgactggta cgcgccgcat gtcgccaagg
     1141 cctcgttgac ctccggcgcg aaggcgagtt ttgtcaatct ctggcggcac gtgctgcgcg
     1201 atatcgtctc cgggccgcaa acctggacgc tacgcgattt ccactcgccg aatatcatct
     1261 ggctcggtga gcggcatggg agcgcgcgcg tcggcctgat cgattttcag gactgcgtgc
     1321 ttggccatcc ggcctacgac gtcgtgtctc ttctgcaaga cgcgcgggtg aacgtgcccg
     1381 cggacgtcga actcaagctc ctcggccatt atatgcaatt gcgccgcatc gccgacgcga
     1441 atttcgatat ggccggcttt gcccgcgcct atgcggttct cggcgcgcag cgcgcgacga
     1501 aaattctcgg aatttttgcg cgtctcgata agcgcgacca caaacctcaa tatctggcac
     1561 atctgccacg cattgaggaa aatttagcca aggatctggc acatcccttg ttgtccggtc
     1621 tcaagggctg gtatgaagcc cacctgcccc agatcttcca acggggcgcg tgaccgggtg
     1681 gatcgcgctc catcctcgga cgcgaactgg gtctgctgcg ggttcgcttt tcgtgtccaa
     1741 gaaatctttc atgccggata aagccatggt ctttgccgcg ggcctcggca cgcgcatgcg
     1801 gccgctgacc gagacagtgc caaaaccgct catcaaggtc ggcgggcggc ccatgatcga
     1861 tcatctgctc gaccggttcg cgcaggctgg cgtcagcacc gccatcgtca acgtgcatca
     1921 tctcgctgat cagatcgaga cgcatctcga cggtcgcagc gcgccgcatg tggtgatctc
     1981 cgacgagcgc gatcttctga tcgatcaggg tggcgcgatc aaaa
//
